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Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
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1

New method studies living bacteria cells  

NLE Websites -- All DOE Office Websites (Extended Search)

New method studies living bacteria cells New method studies living bacteria cells Researchers at the U.S. Department of Energy's Argonne National Laboratory have found a new way to study individual living bacteria cells and analyze their chemistry. In research published today in Science, the scientists used high-energy X-ray fluorescence measurements for mapping and chemical analyses of single free-floating, or planktonic, and surface-adhered, or biofilm, cells of Pseudomonas fluorescens. The results showed differences between the planktonic and adhered cells in morphology, elemental composition and sensitivity to hexavalent chromium, a heavy-metal contaminant and a known carcinogen. The biofilm cells were more tolerant of the contaminant, while it damaged or killed the planktonic cells. Experimental data from sector 1

2

What is the upper size limit for cosmopolitan distribution in free-living microorganisms?  

E-Print Network (OSTI)

What is the upper size limit for cosmopolitan distribution in free-living microorganisms? ABSTRACT distance apart) is used to try and answer the question `What is the upper size limit for cosmopolitan to 230 lm while the largest cosmopolitan species was 135 lm in size. Comparison of the testate

Brown, Richard

3

Method of separating bacteria from free living amoebae  

DOE Patents (OSTI)

New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

Tyndall, Richard L. (Clinton, TN)

1994-01-01T23:59:59.000Z

4

Unexpected photoreactivation of Vibrio harveyi bacteria living in ionization environment  

SciTech Connect

Bacteria undergoing environmental effects is extremely interesting for structural, mechanistic, and evolutionary implications. Luminescent bacteria that have evolved in a specific ambient have developed particular responses and their behavior can give us new suggestions on the task and production of luciferina proteins. To analyze the UV interaction under controlled laboratory conditions, we used photoluminescent bacterial strains belonging to a new species evolutionarily close to Vibrio harveyi sampled from a coastal cave with a high radon content that generates ionizing radiation. The survival of the bacterial strains was analyzed, in the light and in the dark, following a variety of genotoxic treatments including UV radiation exposure. The strains were irradiated by a germicide lamp. The results demonstrated that most of the strains exhibited a low rate of survival after the UV exposure. After irradiation by visible light following the UV exposure, all strains showed a high capability of photoreactivation when grown. This capability was quite unexpected because these bacteria were sampled from a dark ambient without UV radiation. This leads us to hypothesize that the photoreactivation in these bacteria might have been evolved to repair DNA lesions also induced by different radiation sources other than UV (e.g., x-ray) and that the luminescent bacteria might use their own light emission to carry out the photoreactivation. The high capability of photoreactivation of these bacteria was also justified by the results of deconvolution. The deconvolution was applied to the emission spectra and it was able to show evidence of different light peaks. The presence of the visible peak could control the photolysis enzyme.

Alifano, P.; Tala, A.; Tredici, S. M. [Dipartimento Microbiologia, Di.S.Te.B.A., Universita del Salento, via Provinciale Lecce-Monteroni, C.P. 193, 73100 Lecce (Italy); Nassisi, V. [Laboratorio di Elettronica Applicata e Strumentazione, LEAS, Dipartimento di Fisica, Universita del Salento and INFN-Lecce, Via Provinciale Lecce-Monteroni, 73100 Lecce (Italy); Siciliano, M. V. [Laboratorio di Elettronica Applicata e Strumentazione, LEAS, Dipartimento di Fisica, Universita del Salento and INFN-Lecce, Via Provinciale Lecce-Monteroni, 73100 Lecce (Italy); Dipartimento di Scienza dei Materiali, University of Salento, via Provinciale Lecce- Monteroni, C.P. 193, 73100 Lecce (Italy)

2011-05-15T23:59:59.000Z

5

Bacteria eats radioactive waste  

NLE Websites -- All DOE Office Websites (Extended Search)

Bacteria eats radioactive waste Bacteria eats radioactive waste Name: deenaharper Status: N/A Age: N/A Location: N/A Country: N/A Date: Around 1993 Question: In my studies, I have found that everything in this world is balanced. When something dies it is converted into life. Is there anything out there that could convert radioactive material into a harmless substance? Some sort of bacteria that consumes radiation? Replies: The reason why radiation is so harmful is that is produces free radicals in living tissue, that is, it de-stabilizes molecules by tearing off electrons due to intense energies. These free radicals start a chain reaction of destruction, de-stabilizing neighboring molecules. If this continues unchecked, cells die, genetic material are mutated, and tissue aging accelerates. It is somewhat like being burned. Fire oxidizes by a similar free radical reaction. (Hence the term "sun burn.") The natural defenses against free radical reactions in biological systems are antioxidants, which are enzymes, nutrients, and other chemicals, which quench free radical reactions. Without them, life would very quickly cease. To my knowledge, no microorganism has an antioxidant capacity great enough to withstand even minimal exposure to any type of radiation. Microorganisms are actually very susceptible to radiation, which is why heat and gamma irradiation are used to sterilize food, instruments, etc. However, you raise an interesting possibility in that perhaps one can be genetically engineered to have super- antioxidant capacity, but that may be beyond current technology. Plus, if any got loose, given the exponential rate of reproduction, they may become an uncontrollable health hazard, as it would be very difficult to destroy them!

6

Martian bacteria?  

NLE Websites -- All DOE Office Websites (Extended Search)

Martian bacteria? Martian bacteria? Name: clement Status: N/A Age: N/A Location: N/A Country: N/A Date: Around 1993 Question: Is it possible for there to be life of anaerobic bacteria in the ice caps of the planet Mars? Replies: As far as I know, there is no evidence against such life on Mars, so the short answer is: yes. Jade Sure -- except that it would be pretty limited in its lifestyle - - no cable TV for this bug. Because the temperatures on Mars can reach to below -100 C at the poles, life would be extremely difficult, and the lack of nutrients anywhere except from inorganic chemical constituents in the soil or in the ice around the bacterial colonies would keep the menu fairly short. Oh, and do not rule out aerobes -- Mars has an atmosphere, though admittedly not much of one, and there are such organisms as microaerophiles and also microorganisms known as facultative anaerobes, which can grow in the presence of oxygen but which do not need it to survive

7

Cellulolytic Microorganisms from Thermal Environments  

Science Conference Proceedings (OSTI)

Thermal, anaerobic environments rich in decaying plant material are a potential source of novel cellulolytic bacteria. Samples collected from geothermal aquifers in the Yellowstone National Park (YNP) were used to select for cellulolytic thermophiles. Laboratory enrichments on dilute-acid pretreated plant biomass (switchgrass, Populus), and crystalline cellulose (Avicel) resulted in the isolation of 247 environmental clones. The majority of individual clones were affiliated with the cellulolytic bacteria of phylum Firmicutes, followed by xylanolytic and saccharolytic members of the phylum Dictyoglomi. Among the Firmicutes, the clones were affiliated with the genera Caldicellulosiruptor (54.4%), Caloramator (11.5%), Thermoanaerobacter (8.8%), Thermovenabulum (4.1%), and Clostridium (2.0%). From established anaerobic thermophilic enrichments a total of 81 single strains of the genera Caldicellulosiruptor (57%) and Thermoanaerobacter (43%) were isolated. With continuous flow enrichment on Avicel, increases in the relative abundance of Caloramator sp. was observed over clones detected from the Caldicellulosiruptor. Complex communities of interacting microorganisms bring about cellulose decomposition in nature, therefore using up-to-date approaches may yield novel cellulolytic microorganisms with high activity and a rapid rate of biomass conversion to biofuels.

Vishnivetskaya, Tatiana A [ORNL; Raman, Babu [ORNL; Phelps, Tommy Joe [ORNL; Podar, Mircea [ORNL; Elkins, James G [ORNL

2012-01-01T23:59:59.000Z

8

Bacteria: Good or Bad?  

NLE Websites -- All DOE Office Websites (Extended Search)

Bacteria: Good or Bad? Bacteria: Good or Bad? Name: Talei Location: N/A Country: N/A Date: N/A Question: Is bacteria good for you? Replies: both good and bad. Without bacteria we would die, but some bacteria could easily kill us: it all depends which ones they are. The good ones, those we need to survive, are there to digest our food (in our intestines) and to live on our skin and in our mouth. By doing so they provide a protection against bad bacteria, that make you sick, and these are called 'pathogenic bacteria'. Those bugs take their chance if your immune system is weakened, or if they manage to get into your body in large amounts, by contaminated food for instance. Anyway, the simple question has a complicated answer. If you're interested, read more about bacteria, both good and bad, at www.bacteriamuseum.org

9

Genetically Engineered Ethanol Producing Microorganisms ...  

Search PNNL. PNNL Home; About; Research; Publications; Jobs; News; Contacts; Genetically Engineered Ethanol Producing Microorganisms. Battelle ...

10

Response of Prochlorococcus ecotypes to co-culture with diverse marine bacteria  

E-Print Network (OSTI)

Interactions between microorganisms shape microbial ecosystems. Systematic studies of mixed microbes in co-culture have revealed widespread potential for growth inhibition among marine heterotrophic bacteria, but similar ...

Sher, Daniel

11

Genetically Engineered Ethanol Producing Microorganisms ...  

Researchers at PNNL have developed a process concept for the use of microorganisms in the production of fuels, chemicals and other products.

12

Basic Bacteria  

NLE Websites -- All DOE Office Websites (Extended Search)

Basic Bacteria Basic Bacteria Name: Valerie Location: N/A Country: N/A Date: N/A Question: I'm doing a science project on bacteria. WHat I'm doing is washing forks with different dishwashing liquids, then wiping any remaining bacteria on to Agar petri dishes. Then incubating it and seeing which soap removed the most. My question is what kind of bacteria would be growing? and also do I just count the colonies to compare? and how long and at what temperature should I incubate this bacteria? Thank you very much for your time. I'll be looking forward to your response. Replies: The temperature is easy: 37 degrees C is optimal for many bacteria. The medium will determine which bacteria grow best. So if you don't see growth on one medium, but you see growth on another, it tells you that there is a difference in nutrients present in those media that is required for that bacteria. Look at your plates after 24 hr, then put them back in the incubator (keep them sterile) and look at them after 48 hrs--do you see the difference? any slow-growing bacteria visible or did the fast-growing take over the complete plate?

13

Killing Bacteria  

NLE Websites -- All DOE Office Websites (Extended Search)

Killing Bacteria Killing Bacteria Name: alli Location: N/A Country: N/A Date: N/A Question: What kills more bacteria for the recommended cooking time in a microwave and a conventional oven? Replies: I hope I understand your question. The time it takes to get your food done is sufficient to kill all bacteria--but not the spores of certain bacteria--both by microwave cooking and in a conventional oven. The spores are not a problem when the food is consumed directly but can be a health hazard when food is bottled and stored. See recommendations at http://www.cfia-acia.agr.ca/english/corpaffr/foodfacts/perfrine.shtml on Clostridium, one of the most dangerous causes of food poinsoning. Or visit the display on Food Safety in the Virtual Museum of Bacteria (www.bacteriamuseum.org) at www.bacteriamuseum.org/niches/foodsafety/foodsafety.shtml

14

Living in living cities  

Science Conference Proceedings (OSTI)

This article presents an overview of current and potential applications of living technology to some urban problems. Living technology can be described as technology that exhibits the core features of living systems. These features can be useful to solve ... Keywords: Living technology, adaptation, learning, robustness, self-organization, urbanism

Carlos Gershenson

2013-10-01T23:59:59.000Z

15

Microorganisms to Speed Production of Biofuels  

ORNL 2011-G00203/jcn UT-B ID 201002408 08.2011 Microorganisms to Speed Production of Biofuels Technology Summary Researchers at ORNL developed microorganisms that can ...

16

Bacteria Catalog  

NLE Websites -- All DOE Office Websites (Extended Search)

Bacteria Catalog Bacteria Catalog Name: Robin Location: N/A Country: N/A Date: N/A Question: I would just like to have a list brought up of gram neg. and gram pos. bacteria, names of bacteria and what category they fall under. Such as Staphylococcus aureus is gram positive. This would be very helpful in my MBIO LAB. Thank you, student at NSU, central Louisiana. Replies: Your best bet would be to start with looking in the backs of microbiology text books. Many of them have an index with this information. The internet may also be helpful. Saundra Sample Gram positive: Staphylococcus sp., Streptococcus sp., Bacillus sp., Cornyebacterium sp., Clostridium sp. Gram negative: E coli, Pseudomonas sp., Proteus sp., Enterobacter sp., Klebsiella sp., Serratia sp., Citrobacter sp.

17

The Disinfection Efficacy of Chlorine on Sulfate-reducing Bacteria and Iron Bacteria in Water Supply Systems  

Science Conference Proceedings (OSTI)

Sulfate reducing bacteria (SRB) and iron reducing bacteria (IRB) that widely exist in water supply networks are the main microorganisms leading to metal corrosion in pipelines. Chlorine is widely used in drinking water supply systems for sterilization. ... Keywords: Chlorine, SRB, IRB, disinfection efficacy

Qi Beimenr; Wu Chenguang; Chen Xiaoju; Yuan Yixing

2012-05-01T23:59:59.000Z

18

Counting Bacteria  

NLE Websites -- All DOE Office Websites (Extended Search)

Counting Bacteria Counting Bacteria Name: Tammy Location: N/A Country: N/A Date: N/A Question: I am working with my daughter on her Science Fair Project. We are testing daily items that we come in contact with to see how many germs and bacteria it has. How can we differentiate between the types of bacteria? How can we decide which one has the most? We are using the growth medium Agar in petri dishes. Where can I find more scientific info as to why this happens so we can write up the project? Replies: These are complex questions. First, the agar medium is used as a solid phase so that one can see colonies formed. These are round mounds of growth because bacteria multiply in all directions, but they cannot normally move in or on a solid phase so they remain at the site of multiplication. Every bacterial cell can multiply into a colony. Thus, the number of colonies is a measure for the number of cells present, if you have taken quantitative samples. If you want to quantitate, you should try to standardize your samples (for example, use 1 ml liquid to wash surfaces, food particles, 1 ml of liquids, etc. and add of this one drop (with a micropipette would be more accurate) per agar plate and let the drop form a tear on the plate. The number of colonies that grow in this tear are a measure for the original number of bacteria present in the drop, because each colony is derived from a single bacterial cell.

19

Biodegradation of Triclosan by Aerobic Microorganisms  

E-Print Network (OSTI)

Triclosan, a synthetic antimicrobial agent, is an emerging environmental contaminant. Due to incomplete removal of triclosan by wastewater treatment plants, treated wastewater is one major source of environmental triclosan. Biodegradation of triclosan has been observed in activated sludge and the environment, suggesting that it is possible to develop a cost-effective biotreatment strategy for triclosan removal from wastewater. However, current knowledge on triclosan biodegradation is scarce and limited. To bridge this knowledge gap, this dissertation characterized cultivable triclosan-degrading microorganisms, identified uncultivable triclosan-utilizing bacteria, and elucidated triclosan biodegradation pathways. Furthermore, two treatment strategies were examined to enhance triclosan biodegradation in nitrifying activated sludge (NAS). A wastewater bacterial isolate, Sphingopyxis strain KCY1 (hereafter referred as strain KCY1), can completely degrade triclosan with a stoichiometric release of chloride. This strain can retain its degradation ability toward triclosan when after grown in complex nutrient medium containing triclosan as low as 5 micrograms/L. Based on five identified metabolites, a meta-cleavage pathway was proposed for triclosan biodegradation by strain KCY1. By using [13C12]-triclosan stable isotope probing, eleven uncultured triclosan-utilizing bacteria in a triclosan-degrading microbial consortium were identified. These clones are distributed among alpha-, beta-, or gamma-Proteobacteria, suggesting that triclosan-utilizing bacteria are phylogenetically diverse. None of these clone sequences were similar to known triclosan degraders. Growth substrates affected the triclosan degradation potential of four selected oxygenase-expressing bacteria. Biphenyl-grown Burkholderia xenovorans LB400 and propane-grown Rhodococcus ruber ENV425 cannot degrade triclosan. On the other hand, propane- and 2-propanol-grown Mycobacterium vaccae JOB5 can degrade triclosan completely. Due to product toxicity, finite transformation capacities for triclosan were observed for Rhodococcus jostii RHA1 grown on biphenyl, propane, and LB medium with dicyclopropylketone (alkane monooxygenase inducer). Four chlorinated metabolites were detected during triclosan degradation by biphenyl-grown RHA1 and a meta-cleavage pathway was proposed. Complete triclosan (5 mg/L) degradation was observed within 96 hrs in NAS receiving ammonia amendment (0 to 75 mg/L of NH4-N). The fastest triclosan degradation was observed in the NAS exhibiting the highest amount of ammonia. When ammonia oxidation was active in NAS, the amendment of strain KCY1 did not further enhance triclosan removal. Overall, the results suggested that triclosan biodegradation can be enhanced by increasing the activity of ammonia oxidation in NAS.

Lee, Do Gyun

2012-08-01T23:59:59.000Z

20

Bacteria Strains  

NLE Websites -- All DOE Office Websites (Extended Search)

Bacteria Strains Bacteria Strains Name: Michael Location: N/A Country: N/A Date: N/A Question: In the medical setting, how prevalent are strains of Vancomycin-resistant Staphylococcus aureus (bacterium)? Any statistical data would be helpful..... Replies: You can find a report on incidence at http://www.slackinc.com/general/iche/stor1096/thru.htm The Virtual Museum of bacteria, at http://www.bacteriamuseum.org contains general information about bacteria, including antibiotic resistance, and in the near future will contain specialized information on S. aureus Dr. Trudy Wassenaar I don't have those figures for you but you can probably find them at www.CDC.gov. this is the site for the Centers for Disease Control and their job is to keep track of these things. I bet if you go to a search engine (ie yahoo.com, etc.) and search under +"CDC" +"vancomycin resistant Staphylococcus aureus" you might even get to the right page.

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

Bacteria Types  

NLE Websites -- All DOE Office Websites (Extended Search)

Bacteria Types Bacteria Types Name: Evelyn Location: N/A Country: N/A Date: N/A Question: What is the significance of S. marcescens,M.luteus, S.epidermidis, and E. Coli? Which of these are gram-positive and gram-negative, and where can these be found? Also, what problems can they cause? When we culture these bacteria, we used four methods: plates, broth, slants, and pour plates. The media was made of TSB, TSA, NAP, and NAD. What is significant about these culturing methods? Replies: I could give you the answer to that question but it is more informative, and fun, to find out yourself. Start with the NCBI library online (http://www.ncbi.nlm.nih.gov/) and do a query with the species name, and 'virulence' if you want to know what they're doing to us. Have a look at the taxonomy devision to see how they are related. To find out if they're gram-pos or neg you should do a gram stain if you can. Otherwise you'll find that information in any bacteriology determination guide. Your question about the media is not specific enough so I can't answer it.

22

Bacteria Odors  

NLE Websites -- All DOE Office Websites (Extended Search)

Bacteria Odors Bacteria Odors Name: Jason Location: N/A Country: N/A Date: N/A Question: I am doing a science project on food decomposition. I would like to know why food -- especially meat--smells bad when it goes bad. Also Why does bread grow mold and why is it green and blue? Thank you. Replies: Jason, When food is being eaten by bacteria, they produce byproducts just like we do. The compounds they produce cause the smell of rotten meat. There is a reason we don't like that smell: it warns us that that food is probably unsafe to eat. So biology has built in a safety rule: you would not normally eat something that you don't like the smell of. The molds on bread are a special kind that like bread. Their spores are everywhere but only when we leave our bread long enough can they grow to sufficient numbers so that we can see them. Again, you can smell the bread is off, you can see it, and if you hadn't noticed you will taste it. However you should not eat bread with molds on: they produce toxic substances. I don't think there is a reason for them to be green and blue, at least I don't know it.

23

Stomach Bacteria  

NLE Websites -- All DOE Office Websites (Extended Search)

Stomach Bacteria Stomach Bacteria Name: pam Location: N/A Country: N/A Date: N/A Question: what kind of bacteria or parasite etc. can come from the water and looks like a hairlike is sticky and gets in your tetth causes stomach problems. and when it is just out of my mouth squishy and when it is dry it scrivals up and is hard. has made my newborn son sick ky daughters sick . why is it that you can litearly put hand soap or anti-bacterial on and within seconds you can see what i call blue hairs. or it seems to have gotten bigger and is is whit or half black white?they are in my snot and flem. in my kids bowel movement i think i have contamiated water but no one believes me. it reminds me of a worm .i have to black things attached to twwo of myteeth (looks like a littli tick) can you halp me or am t really crazy? if you could recommend some one or somplace to help me.

24

Water Treatment Strategies: Microorganism Control  

Science Conference Proceedings (OSTI)

This report presents an overview of the fundamental concepts of microorganism control and a discussion about how these concepts can be applied for optimizing current prevention and mitigation strategies in nuclear power plant service water systems. A database has been established to facilitate development of treatment and operation strategies that meet the requirement for preventing microbiological problems while overcoming limitations with current water treatment technologies.

2004-12-20T23:59:59.000Z

25

Microorganisms to Speed Production of Biofuels - Energy ...  

Researchers at ORNL developed microorganisms that can quickly overcome the resistance of biomass to breakdown, and improved both the cost and ...

26

Cofermentation with Cooperative Microorganisms for More Efficient ...  

ORNL 2011-G00205/jcn UUT-B ID 201002454 09.2011 Cofermentation with Cooperative Microorganisms for More Efficient Biomass Conversion Technology Summary

27

A combined microfluidic/dielectrophoretic microorganism concentrator  

E-Print Network (OSTI)

This thesis presents the development of a high-throughput microfluidic microorganism concentrator for pathogen detection applications. Interdigitated electrodes lining the bottom of the channel use positive dielectrophoretic ...

Gadish, Nitzan

2005-01-01T23:59:59.000Z

28

Cellulase producing microorganism ATCC 55702  

DOE Patents (OSTI)

Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

Dees, H. Craig (Lenoir City, TN)

1997-01-01T23:59:59.000Z

29

New Sampling Methods for Airborne Microorganisms  

NLE Websites -- All DOE Office Websites (Extended Search)

New Sampling Methods for Airborne Microorganisms New Sampling Methods for Airborne Microorganisms Speaker(s): Klaus Willeke Date: February 27, 2001 - 12:00pm Location: Bldg 90 Seminar Host/Point of Contact: David Faulkner Klaus Willeke and his international team of engineers, physicists, microbiologists, industrial hygienists and environmental scientists have worked for about 15 years on the development of new methods for sampling airborne microorganisms. The following topics will be highlighted: long-term bioaerosol sampling into liquid by swirling air motion ("Biosampler"); personal aerosol sampling with low wind sensitivity and highfilter deposit uniformity ("Button Aerosol Sampler"); collection of microorganisms by electrostatic means; source testing as a predictor for microorganism release from surfaces; particle concentrating from large air

30

Why Sequence Bacteria from Stromatolites?  

NLE Websites -- All DOE Office Websites (Extended Search)

Bacteria from Stromatolites? Bacteria from Stromatolites? Marine stromatolites are formed by the interactions of several key bacterial groups, which precipitate repeating layers (laminae) of calcium carbonate (CaCO3). During 70% of the time life has occupied earth, stromatolites were a dominant biological community. Their associated microbial communities have played a significant role in carbon sequestration, preservation, and cycling during the evolution of life. Present-day marine stromatolite communities consist of cyanobacteria (both free-living filamentous cyanobacteria and coccoid endoliths), sulfate reducers (SRB), sulfur-oxidizers (SOB), and aerobic heterotrophs (including fermenters). The interactions of these key groups drive the organized precipitation of CaCO3. The marine stromatolite system, therefore, provides

31

Bacteria isolated from amoebae/bacteria consortium  

DOE Patents (OSTI)

New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

Tyndall, Richard L. (Clinton, TN)

1995-01-01T23:59:59.000Z

32

Material to Efficiently and Economically Obtain Microorganism and Microalgae  

Technology provides an economical and efficient process to harvest microorganisms like microalgae from its growth media.

33

Hydrogen Based Bacteria  

NLE Websites -- All DOE Office Websites (Extended Search)

Hydrogen Based Bacteria Hydrogen Based Bacteria Name: Ellen Location: N/A Country: N/A Date: N/A Question: i was in my Biology class and a very respectable someone mentioned something about the discovery of a hydrogen based bacteria. my teacher wasnt aware of this study, and assigned me to find out about it. so i thought i would Email you and see if you people knew anything about it. Awaiting your repsonse Replies: I'm not quite sure what you mean by hydrogen based bacteria but I will take a stab that you mean bacteria that use hydrogen for energy. Some bacteria are chemolithotrophs which mean that they are autrophs but don't use the sun as their energy source; they get their energy from chemical sources. There are bacteria that use hydrogen as their energy source. They are diverse as a group and are all facultative. The overall chemical reaction looks like this:

34

Phylogenetic Distribution of Potential Cellulases in Bacteria  

E-Print Network (OSTI)

Project Goals: The main goal of this project is to connect diverse microbial groups with the extracellular enzyme systems that catalyze the decay of organic material. We will also determine whether different groups of microbes and their enzymes respond to environmental changes, and whether they can recover from such changes. Finally, we will develop mathematical models to predict the responses of microbial communities and their associated functions under new environmental conditions. In most terrestrial ecosystems, the depolymerization of plant cell wall is the rate limiting step in the turnover of organic material. The composition of plant detritus is known to depend mainly on enzymes produced by microorganisms. This raises the question: which phylogenetic lineages of microorganisms can degrade plant cell wall material, including cellulose? To address this question, we compared the distribution of Glycoside Hydrolases (GH) potentially related to the cellulose degradation among 3744 bacteria. Some phylogenetic groups are especially rich in GHs whereas some are very poor. For example, in bacteria from the Bacteroidetes phylum ~40 GHs (from the families 1,

Genomic Science Awardee; Renaud Berlemont

2012-01-01T23:59:59.000Z

35

Flesh Eating Bacteria  

NLE Websites -- All DOE Office Websites (Extended Search)

Flesh Eating Bacteria Flesh Eating Bacteria Name: Jennifer Location: N/A Country: N/A Date: N/A Question: I was wondering what the inovations for treating the flesh eating bacteria are if there are any.... Replies: "Flesh eating bacteria" are Streptococci, the ones that cause throat infections but sometimes cause extremely severe subcutaneous (below the skin) infections.In order to do this they have to enter the body through a damaged skin: a cut would be enough The infection is treated with antibiotics and hospitalization is needed for this life-threatening infection. Find more info at http://www.acrylicbath.com/info.html If you want to know more about bacteria, take a look at http://www.bacteriamuseum.org In this site you can find general information about bacteria, both pathogenic and 'good'. Have fun!

36

Recombinant microorganisms for increased production of organic acids  

DOE Patents (OSTI)

Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

Yi, Jian; Kleff, Susanne; Guettler, Michael V

2013-04-30T23:59:59.000Z

37

Recombinant microorganisms for increased production of organic acids  

DOE Patents (OSTI)

Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

Yi, Jian (East Lansing, MI); Kleff, Susanne (East Lansing, MI); Guettler, Michael V. (Holt, MI)

2012-02-21T23:59:59.000Z

38

(Bioprocessing of lignite coals using reductive microorganisms)  

SciTech Connect

The objectives of this report are to: (1) characterize selected aerobic bacterial strains for their abilities to depolymerize lignite coal polymers, and isolate and identify the extracellular enzymes responsible for depolymerization of the coal; (2) characterize selected strictly anaerobic bacteria, that were previously shown to reductively transform coal substructure model compounds, for the ability to similarly transform polymeric coal; and (3) isolate more strains of anaerobic bacteria by enrichment using additional coal substructure model compounds and coal as substrates.

Crawford, D.L.

1990-01-01T23:59:59.000Z

39

Ampicillin and Bacteria  

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Ampicillin and Bacteria Ampicillin and Bacteria Name: sara Location: N/A Country: N/A Date: N/A Question: could you please explain fully how ampicillian destroys E.coli cells? Replies: Ampicillin is an antibiotic belonging to the group of beta-lactam antibiotics. These will kill Gram-negative bacteria to which E.coli and Salmonella belong. The antibiotic prevents the formation of peptidoglycan, an essential building block of the cell membrane. So the antibiotic prevents growth of cells. You can read more about antibiotics and how bacteria can become resistant against them at the Virtual Bacteria of Bacteria: http://www.bacteriamuseum.org Dr. Trudy wasenaar Unfortunately, I don't have my micro books with me, but I'll try. Bacteria have a unique compound in their cell walls called peptidoglycan. It is made of 2 types of sugar residues that are cross-linked (like a chain link fence) by tetrapeptides. Penicillin prevents the crosslinking of the sugars by breaking the tetrapeptides. These antibiotics are more effective against gram positive organisms (such as Staph.) than gram negative organisms (such as E coli) because gram pos. have more peptidoglycan in their cell walls. Some bacteria have adapted to this situation by having an enzyme called beta-lactamase that breaks the structure of the antibiotic open rendering it ineffective. I'm pretty sure ampicillin is a penicillin derivative and therefore has the same mode of action, but I would check to make sure if I were you.

40

Microwaves and Bacteria  

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Microwaves and Bacteria Microwaves and Bacteria Name: mike Location: N/A Country: N/A Date: N/A Question: Do the microwaves in a microwave kill bacteria or is it the heat that kills the bacteria? I am wondering this because i have a science fair project and i am searching for a project dealing with bacteria. Replies: As far as I'm aware it is the heat that kill bacteria in a microwave, and they need quite some time to be dead. The spores that some kind of bacteria make to survive harsh conditions do not contain much water and they might survive microwaves. I'm not sure what bacteria do that can survive high dosis of radiation, like Deinococcus radiodurans. They can do this by a very efficient repair system for their DNA. My guess is that they would also be killed by the heat generated in a microwave but I haven't found any data on this.

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

Why Sequence Anammox Bacteria?  

NLE Websites -- All DOE Office Websites (Extended Search)

Anammox Bacteria? Anammox Bacteria? Micrograph courtesy Mike S. Jetten, Radboud Univ. The deep sediments and oxygen minimum zones of the world's oceans are assumed to be responsible for the majority of nitrogen loss on earth. The microbes responsible for the nitrogen loss were long unknown, but compelling evidence is now accumulating that marine anaerobic ammonium oxidizing (anammox) bacteria are responsible, making them very important players in the global nitrogen cycle. In marine ecosystems, the carbon and nitrogen cycles are closely connected. More knowledge of the regulation and mechanism of CO2 sequestration by anammox bacteria in the ocean will contribute to our understanding of the global biogeochemical cycles and their impact on climate change. Anammox bacteria are also able to synthesize the rocket fuel hydrazine from

42

Cat Dish Bacteria Determination  

NLE Websites -- All DOE Office Websites (Extended Search)

Dish Bacteria Determination Dish Bacteria Determination Name: Ashlyn Status: student Grade: 6-8 Location: FL Country: USA Date: Summer 2011 Question: Is there a simple way to measure bacteria in cat's water dishes without doing something with Agar? To measure bacteria in a water bowl, do I need to use a microscope? I am thinking of using different materials (metal, plastic, and glass) to see which of those has the grows the most bacteria. Replies: Hello Ashlyn, That is a very good idea for a science project. Usually the best way to do a quantitative analysis of bacteria content is to take a measured amount of a liquid, plate it out on some type of agar and do a colony count. This will give a basic indication of bacterial load, but not differentiate the types of bacteria. The most common type of bacteria that causes a pink film to form on water bowls and showers, etc. is Serratia marcescens. It is a fairly harmless organism that reacts with standing water. It may only adhere to the walls of the container and not be 'free floating' in the water. A microscope would not likely help unless you were able to do special stains to help see the bacteria. You might also want to add stoneware or ceramic to your list. Just so you know stoneware or ceramic make the best containers for cats to drink out of. It keeps the water fresher: Maybe less bacteria? You might just have to rely on a visual inspection of the containers to see which has more pink per surface area.

43

Live pathogens: rapid detection technique developed  

NLE Websites -- All DOE Office Websites (Extended Search)

January » January » Live Pathogens: Rapid Detection Technique Developed Live pathogens: rapid detection technique developed The technique relies on bacteria being critically dependent upon the key nutrient iron. January 24, 2013 Colorized scanning electron micrograph of E. coli. Colorized scanning electron micrograph of E. coli. Photo credit: US Centers for Disease Control and Prevention LANL's new method eliminates the need for laboratory culture and greatly speeds the process. Los Alamos researchers have developed a better technique for quick detection of live pathogens in the field. Identification of viable bacteria in a complex environment is scientifically challenging. Current detection and diagnostic techniques are inadequate in major public health emergencies, such as outbreaks of food-borne illness. Detection of live

44

Lima Bean Bacteria  

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Lima Bean Bacteria Suspension: We place 1-2 handfuls of dry lima beans in a large jar and fill halfway to the top with distilled water. Then, covered and sat in a warn room...

45

Insulin and Bacteria  

NLE Websites -- All DOE Office Websites (Extended Search)

Insulin and Bacteria Insulin and Bacteria Name: sid Location: N/A Country: N/A Date: N/A Question: Can you explain Insulin production in bacteria and its regulation by IPTG. Replies: Information on expression of eukaryotic genes in bacteria can be found in any molecular biology textbook. See for instance 'Recombinant DNA by Watson, Gilman, Witkowski and Zoller, 2nd Ed., chapter 23 where the cloning of insulin is described. The use of an IPTG inducable promoter (the promoter of LacZ is only active when the inductor IPTG is present) is also explained in text books, and even in catalogs of the companies selling the plasmids that are used for such experiments. With a bit of reading you'll become an expert! Dr. Wassenaar I'm not sure what IPTG is. But are you referring to recombinant DNA technology? First a human gene for insulin was isolated and cut out of the human chromosome with restriction enzymes. These are enzymes that cut DNA at very specific spots in the DNA. They are like DNA scissors. Then a small piece of DNA called a plasmid is isolated from a bacteria. The same restriction enzyme is used to cut the plasmid. The insulin gene from the human is inserted into the bacterial DNA and they are sealed together with an enzyme called ligase. The plasmid is reinserted into the bacteria and the bacteria will treat the human insulin gene as its own. When it comes time to make protein it will make the insulin as well. Bacteria reproduce very rapidly and are easily maintained. We can get vats of human insulin by this method.

46

Storing data encoded DNA in living organisms  

DOE Patents (OSTI)

Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

Wong; Pak C. (Richland, WA), Wong; Kwong K. (Sugar Land, TX), Foote; Harlan P. (Richland, WA)

2006-06-06T23:59:59.000Z

47

Mechanisms of oil displacement by microorganisms  

SciTech Connect

Mechanisms of oil displacement at the oil-brine-sand interfaces by bacteria were investigated by microscopic observations and capillary pressure changes using unconsolidated, thin, reservoir flow cells. Three genera of bacteria, Bacillus, Pseudomonas, and Clostridium, were injected into water-wet and oil-wet cells of unconsolidated sand, saturated with brine and crude oil. The flow cells were placed under a microscope for visual and photographic observations. The flow cells were connected to a manometer to examine the complete capillary pressure hysteresis loop before and after introduction of bacteria. The thin reservoir flow cells were first saturated with brine, and then displaced to irreducible water saturation with crude oil. Oil and brine displacements were then made to determine the complete capillary pressure relationship. Next, the bacteria and nutrients were introduced and incubated in the flow cell for 24-48 hours. Microscopic observations were recorded photographically. After incubation, oil and brine displacements were again made to determine the capillary pressure hysteresis loop.

Kianipey, S.A.; Donaldson, E.C.

1986-01-01T23:59:59.000Z

48

Arm Pit Bacteria  

NLE Websites -- All DOE Office Websites (Extended Search)

Arm Pit Bacteria Arm Pit Bacteria Name: Kayla Location: N/A Country: N/A Date: N/A Question: I am doing a science fair project on deodorants and anti-perspirants- to see which one hinders bacterial growth more effectively. Here's my problem, I cannot find what kind of bacteria (mainly) flourishes in the axilla (armpit) region. I have been on many different search engines- medical pages- and microbiology pages, and unfortuantely cannot come up with any answers. My school's science fair is March 3rd- so I am in desperate need of an answer!!! I need to be able to order the type of bacteria soon. Thank you. Replies: Try Staphylococcus epidermidis-it is a common organism that grows on the skin. It is not pathogenic (disease-causing) and can be used by students. You could try culturing your own armpit-use a sterile q-tip. Spread the q-tip on the agar plate and then take some of your antiperspirant or deodorant and make a dot in the middle of the plate. Incubate the plate and see if the bacteria are repelled or are resistant. I would measure the size of the zone so you can compare each type of deodorant, etc. You could also check if different people's bacteria are more or less resistant, ie if the same deodorant works for everyone. Each person's bacterial population are a little different. This would require volunteers who would be willing to stick a q-tip in their armpit! If you decide to do this, I would sample the armpit when it is moist, before a shower (dry, clean skin won't have as much bacteria). Good luck.

49

Live Status  

NLE Websites -- All DOE Office Websites (Extended Search)

For Users For Users Live Status Global Queue Look Scheduled Outages Outage Log Edison Login Node Status Hopper Login Node Status Hopper User Environment Monitoring Carver Login Node Status PDSF Login Node Status PDSF Monitoring Science Gateway Status Now Computing Highlights My NERSC Getting Started Computational Systems Data & File Systems Network Connections Queues and Scheduling Job Logs & Analytics Training & Tutorials Software Accounts & Allocations Policies Data Analytics & Visualization Data Management Policies Science Gateways User Surveys NERSC Users Group User Announcements Help Operations for: Passwords & Off-Hours Status 1-800-66-NERSC, option 1 or 510-486-6821 Account Support https://nim.nersc.gov accounts@nersc.gov 1-800-66-NERSC, option 2 or 510-486-8612

50

Fungus or bacteria?  

NLE Websites -- All DOE Office Websites (Extended Search)

Fungus or bacteria? Fungus or bacteria? Name: Gordon T Davis Location: N/A Country: N/A Date: N/A Question: My biology class is growing bacteria cultures taken from different areas on the school campus. How can we accurately classify bacteria from fungus in the petri dish? Replies: How advanced is your class? In general, fungi look like they are made of tiny threads, or they appear to have tiny dots on top of the colony. But this isn't fool-proof. There are lots of books out with good pictures of various bacteria and fungi; you need to be careful, though, since you could end up growing some things that you'd rather not have everyone exposed to - be sure to sterilize the culture dishes with bleach or in a pressure cooker (depending on the type of plate you are using!) before throwing them away. Also be sure that everyone uses sterile techniques, washes their hands thoroughly, doesn't get too close to the dishes with their faces, etc.

51

Testing for Bacteria  

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Testing for Bacteria Testing for Bacteria Name: Danielle Location: N/A Country: N/A Date: N/A Question: I have to do a science fair project. What I need to compare the relative bacterial levels in milk samples with different expiration dates. What can I use to test the bacteria? Please help! Replies: You want to see if there are bacteria present in milk of different expiration dates, and if so, what they do to the milk. So buy milk of different dates. You could test what effect storage temperature has on bacterial growth in milk. Use those different milk samples in their original package (don't open it!) and keep them at 37 degrees C, at room temperature, and in the fridge (measure how cold that is) for say 48 hrs.Then open the bottle or pack and see what happened to the milk. Do you see differences? Can you explain them? If you open the milk before the experiment you may get bacteria into the milk that otherwise would not have been there. The experiment with the closed bottles will tell you what is better, storage of milk at room temperature, warm, or cold.

52

Lactose intolerant bacteria  

NLE Websites -- All DOE Office Websites (Extended Search)

Lactose intolerant bacteria Lactose intolerant bacteria Name: Carolyn McPherson Age: N/A Location: N/A Country: N/A Date: N/A Question: A student scientist is looking for a method to induce lactose intolerance in E. coli bacteria. Is there a suggestion for a method? Thanks Replies: The prevailing wisdom among geneticists is that we cannot really "induce" lactose intolerance so much a we can "select" or "screen" for mutant bacteria that have become lactose intolerant. The idea is that mutations in particular genes do not occur as a response to some evolutionary or selective pressure; rather, mutations just happen randomly, and those that confer a growth advantage (in a particular situation) will help the bacterium grow faster than its siblings. In a previous session, I suggested one way to identify those bacteria; to induce mutations, any of several mutation-causing agents might be tried, including chemicals and UV light. In fact, one worthwhile experiment might be to see how various exposure to UV light might yield increasing numbers of lactose-intolerant mutants. For more details or discussion, you might write me directly (S. Triezenberg).

53

Apparatus and method for the desulfurization of petroleum by bacteria  

DOE Patents (OSTI)

A method is described for treating petroleum with anaerobic microorganisms acting as biocatalysts that can remove sulfur atoms from hydrocarbon molecules, under anaerobic conditions, and then convert the sulfur atoms to hydrogen sulfide. The microorganisms utilized are from the family known as the ``Sulfate Reducing Bacteria``. These bacteria generate metabolic energy from the oxidation of organic compounds, but use oxidized forms of sulfur as an electron acceptor. Because the biocatalyst is present in the form of bacteria in an aqueous suspension, whereas the reacting substrate consists of hydrocarbon molecules in an organic phase, the actual desulfurization reaction takes place at the aqueous-organic interphase. To ensure adequate interfacial contacting and mass transfer, a biphasic electrostatic bioreactor system is utilized. The bioreactor is utilized to disperse and recoalesce a biocatalyst contained in the aqueous liquid phase into the organic liquid phase containing the sulfur. High-intensity electrical fields rupture the aqueous drops into a plurality of microdroplets and induce continuous coalescence and redispersion as the microdroplets travel through the organic phase, thus increasing surface area. As the aqueous microdroplets progress through the organic phase, the biocatalyst then reacts with the sulfur to produce hydrogen sulfide which is then removed from the bioreactor. The organic liquid, now free of the sulfur, is ready for immediate use or further processing. 5 figs.

Lizama, H.M.; Scott, T.C.; Scott, C.D.

1995-10-17T23:59:59.000Z

54

Apparatus and method for the desulfurization of petroleum by bacteria  

SciTech Connect

A method for treating petroleum with anaerobic microorganisms acting as biocatalysts that can remove sulfur atoms from hydrocarbon molecules, under anaerobic conditions, and then convert the sulfur atoms to hydrogen sulfide. The microorganisms utilized are from the family known as the "Sulfate Reducing Bacteria." These bacteria generate metabolic energy from the oxidation of organic compounds, but use oxidized forms of sulfur as an electron acceptor. Because the biocatalyst is present in the form of bacteria in an aqueous suspension, whereas the reacting substrate consists of hydrocarbon molecules in an organic phase, the actual desulfurization reaction takes place at the aqueous-organic interphase. To ensure adequate interfacial contacting and mass transfer, a biphasic electrostatic bioreactor system is utilized. The bioreactor is utilized to disperse and recoalesce a biocatalyst contained in the aqueous liquid phase into the organic liquid phase containing the sulfur. High-intensity electrical fields rupture the aqueous drops into a plurality of microdroplets and induce continuous coalescence and redispersion as the microdroplets travel through the organic phase, thus increasing surface area. As the aqueous microdroplets progress through the organic phase, the biocatalyst then reacts with the sulfur to produce hydrogen sulfide which is then removed from the bioreactor. The organic liquid, now free of the sulfur, is ready for immediate use or further processing.

Lizama, Hector M. (Knoxville, TN); Scott, Timothy C. (Knoxville, TN); Scott, Charles D. (Oak Ridge, TN)

1995-01-01T23:59:59.000Z

55

Engineered microorganisms capable of producing target compounds under anaerobic conditions  

DOE Patents (OSTI)

The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

Buelter, Thomas (Denver, CO); Meinhold, Peter (Denver, CO); Feldman, Reid M. Renny (San Francisco, CA); Hawkins, Andrew C. (Parker, CO); Urano, Jun (Irvine, CA); Bastian, Sabine (Pasadena, CA); Arnold, Frances (La Canada, CA)

2012-01-17T23:59:59.000Z

56

Bioprocessing of lignite coals using reductive microorganisms  

Science Conference Proceedings (OSTI)

In order to convert lignite coals into liquid fuels, gases or chemical feedstock, the macromolecular structure of the coal must be broken down into low molecular weight fractions prior to further modification. Our research focused on this aspect of coal bioprocessing. We isolated, characterized and studied the lignite coal-depolymerizing organisms Streptomyces viridosporus T7A, Pseudomonas sp. DLC-62, unidentified bacterial strain DLC-BB2 and Gram-positive Bacillus megaterium strain DLC-21. In this research we showed that these bacteria are able to solubilize and depolymerize lignite coals using a combination of biological mechanisms including the excretion of coal solublizing basic chemical metabolites and extracellular coal depolymerizing enzymes.

Crawford, D.L.

1992-03-29T23:59:59.000Z

57

Fuel from Bacteria, CO2, Water, and Solar Energy: Engineering a Bacterial Reverse Fuel Cell  

Science Conference Proceedings (OSTI)

Electrofuels Project: Harvard is engineering a self-contained, scalable Electrofuels production system that can directly generate liquid fuels from bacteria, carbon dioxide (CO2), water, and sunlight. Harvard is genetically engineering bacteria called Shewanella, so the bacteria can sit directly on electrical conductors and absorb electrical current. This current, which is powered by solar panels, gives the bacteria the energy they need to process CO2 into liquid fuels. The Harvard team pumps this CO2 into the system, in addition to water and other nutrients needed to grow the bacteria. Harvard is also engineering the bacteria to produce fuel molecules that have properties similar to gasoline or diesel fuelmaking them easier to incorporate into the existing fuel infrastructure. These molecules are designed to spontaneously separate from the water-based culture that the bacteria live in and to be used directly as fuel without further chemical processing once theyre pumped out of the tank.

None

2010-07-01T23:59:59.000Z

58

Why sequence purple sulfur bacteria?  

NLE Websites -- All DOE Office Websites (Extended Search)

purple sulfur bacteria? purple sulfur bacteria? The process by which plants and some bacteria can convert light energy to sugar, or photosynthesis, is crucial to global food webs, and complicated. Very little is known about the photosynthetic bacteria in the purple sulfur bacteria group, which may represent one of the most primitive photosynthetic organisms and are capable of carbon fixation and sequestration in both light and dark conditions with the help of sulfur compounds. Purple sulfur bacteria are autotrophic and can synthesize organic compounds from inorganic sources. Researchers hope to learn more by sequencing nine type strains of purple sulfur bacteria that are found in freshwater, brackish and marine systems. The information would lead to a better understanding of the process of photosynthesis as well as the global

59

Effects of selected thermophilic microorganisms on crude oils at elevated temperatures and pressures. Final report  

Science Conference Proceedings (OSTI)

During the past several years, a considerable amount of work has been carried out showing that microbially enhanced oil recovery (MEOR) is promising and the resulting biotechnology may be deliverable. At the Brookhaven National Laboratory (BNL), systematic studies have been conducted which dealt with the effects of thermophilic and thermoadapted bacteria on the chemical and physical properties of selected types of crude oils at elevated temperatures and pressures. Particular attention was paid to heavy crude oils from Venezuela, California, Alabama, Arkansas, Wyoming, Alaska, and other oil producing areas. Current studies indicate that during the biotreatment several chemical and physical properties of crude oils are affected. The oils are (1) emulsified; (2) acidified; (3) there is a qualitative and quantitative change in light and heavy fractions of the crudes; (4) there are chemical changes in fractions containing sulfur compounds; (5) there is an apparent reduction in the concentration of trace metals; (6) the qualitative and quantitative changes appear to be microbial species dependent; and (7) there is a distinction between {open_quotes}biodegraded{close_quotes} and {open_quotes}biotreated{close_quotes} oils. Preliminary results indicate the introduced microorganisms may become the dominant species in the bioconversion of oils. These studies also indicate the biochemical interactions between crude oils and microorganisms follow distinct trends, characterized by a group of chemical markers. Core-flooding experiments have shown significant additional crude oil recoveries are achievable with thermophilic microorganisms at elevated temperatures similar to those found in oil reservoirs. In addition, the biochemical treatment of crude oils has technological applications in downstream processing of crude oils such as in upgrading of low grade oils and the production of hydrocarbon based detergents.

Premuzic, E.T.; Lin, M.S.

1995-07-01T23:59:59.000Z

60

Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism  

DOE Patents (OSTI)

A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H.sub.2 O and/or CO.sub.2 and H.sub.2 in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate.

Gaddy, James L. (Fayetteville, AR); Clausen, Edgar C. (Fayetteville, AR)

1992-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism  

DOE Patents (OSTI)

A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H[sub 2]O and/or CO[sub 2] and H[sub 2] in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate. 3 figs.

Gaddy, J.L.; Clausen, E.C.

1992-12-22T23:59:59.000Z

62

Biofuels from Solar Energy and Bacteria: Electrofuels Via Direct Electron Transfer from Electrodes to Microbes  

Science Conference Proceedings (OSTI)

Electrofuels Project: UMass is feeding renewable electricity to bacteria to provide the microorganisms with the energy they need to turn carbon dioxide (CO2) directly into liquid fuels. UMass energy-to-fuels conversion process is anticipated to be more efficient than current biofuels approaches in part because this process will leverage the high efficiency of photovoltaics to convert solar energy into electricity. UMass is using bacteria already known to produce biofuel from electric current and CO2 and working to increase the amount of electric current those microorganisms will accept and use for biofuels production. In collaboration with scientists at University of California, San Diego, the UMass team is also investigating the use of hydrogen sulfide as a source of energy to power biofuel production.

None

2010-07-01T23:59:59.000Z

63

Selective microorganism concentration using a dielectrophoresis-based microfabricated device  

E-Print Network (OSTI)

Detection of pathogenic microorganisms is a significant challenge in medicine, environmental protection and biological threat safety because samples are often contaminated. This work presents a method of separating bacterial ...

Pucha?a, Katarzyna Anna

2007-01-01T23:59:59.000Z

64

Bacteria in Permafrost  

DOE Green Energy (OSTI)

Significant numbers of viable ancient microorganisms are known to be present within the permafrost. They have been isolated in both polar regions from the cores up to 400 m deep and ground temperatures of -27 C. The age of the cells corresponds to the longevity of the permanently frozen state of the soils, with the oldest cells dating back to {approx}3 million years in the Arctic, and {approx}5 million years in the Antarctic. They are the only life forms known to have retained viability over geological time. Thawing of the permafrost renews their physiological activity and exposes ancient life to modern ecosystems. Thus, the permafrost represents a stable and unique physicochemical complex, which maintains life incomparably longer than any other known habitats. If we take into account the depth of the permafrost layers, it is easy to conclude that they contain a total microbial biomass many times higher than that of the soil cover. This great mass of viable matter is peculiar to permafrost only.

Gilichinsky, David A [Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences; Vishnivetskaya, Tatiana A [ORNL; Petrova, Maya A [Institute of Molecular Genetics, Russian Academy of Sciences; Spirina, Elena V [Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences; Mamikin, Vladimir [Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences; Rivkina, Elizaveta [Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences

2008-01-01T23:59:59.000Z

65

Engineering Biofuels from Photosynthetic Bacteria  

interdisciplinary target molecule selection and testing ... Engineering Biofuels from Photosynthetic Bacteria (IN 09001) November 2012 tdc_es_in09001_1112_mn

66

Biofuels from Bacteria, Electricity, and CO2: Biofuels from CO2 Using Ammonia or Iron-Oxidizing Bacteria in Reverse Microbial Fuel Cells  

SciTech Connect

Electrofuels Project: Electrofuels Project: Columbia University is using carbon dioxide (CO2) from ambient air, ammoniaan abundant and affordable chemical, and a bacteria called N. europaea to produce liquid fuel. The Columbia University team is feeding the ammonia and CO2 into an engineered tank where the bacteria live. The bacteria capture the energy from ammonia and then use that energy to convert CO2 into a liquid fuel. When the bacteria use up all the ammonia, renewable electricity can regenerate it and pump it back into the systemcreating a continuous fuel-creation cycle. In addition, Columbia University is also working with the bacteria A. ferrooxidans to capture and use energy from ferrous iron to produce liquid fuels from CO2.

2010-07-01T23:59:59.000Z

67

Expansion of the Genomic Encyclopedia of Bacteria and Archaea  

SciTech Connect

To date the vast majority of bacterial and archaeal genomes sequenced are of rather limited phylogenetic diversity as they were chosen based on their physiology and/ or medical importance. The Genomic Encyclopedia of Bacteria and Archaea (GEBA) project (Wu et al. 2009) is aimed at systematically filling the gaps of the tree of life with phylogenetically diverse reference genomes. However more than 99 percent of microorganisms elude current culturing attempts, severely limiting the ability to recover complete or even partial genomes of these largely mysterious species. These limitations gave rise to the GEBA uncultured project. Here we propose to use single cell genomics to massively expand the Genomic Encyclopedia of Bacteria and Archaea by targeting 80 single cell representatives of uncultured candidate phyla which have no or very few cultured representatives. Generating these reference genomes of uncultured microbes will dramatically increase the discovery rate of novel protein families and biological functions, shed light on the numerous underrepresented phyla that likely play important roles in the environment, and will assist in improving the reconstruction of the evolutionary history of Bacteria and Archaea. Moreover, these data will improve our ability to interpret metagenomics sequence data from diverse environments, which will be of tremendous value for microbial ecology and evolutionary studies to come.

Rinke, Christian; Sczyrba, Alex; Malfatti, Stephanie; Lee, Janey; Cheng, Jan-Fang; Stepanauskas, Ramunas; Eisen, Jonathan A.; Hallam, Steven; Inskeep, William P.; Hedlund, Brian P.; Sievert, Stefan M.; Liu, Wen-Tso; Tsiamis, George; Hugenholtz, Philip; Woyke, Tanja

2011-06-02T23:59:59.000Z

68

Expansion of the Genomic Encyclopedia of Bacteria and Archaea  

Science Conference Proceedings (OSTI)

To date the vast majority of bacterial and archaeal genomes sequenced are of rather limited phylogenetic diversity as they were chosen based on their physiology and/ or medical importance. The Genomic Encyclopedia of Bacteria and Archaea (GEBA) project (Wu et al. 2009) is aimed to systematically filling the gaps of the tree of life with phylogenetically diverse reference genomes. However more than 99percent of microorganisms elude current culturing attempts, severely limiting the ability to recover complete or even partial genomes of these largely mysterious species. These limitations gave rise to the GEBA uncultured project. Here we propose to use single cell genomics to massively expand the Genomic Encyclopedia of Bacteria and Archaea by targeting 80 single cell representatives of uncultured candidate phyla which have no or very few cultured representatives. Generating these reference genomes of uncultured microbes will dramatically increase the discovery rate of novel protein families and biological functions, shed light on the numerous underrepresented phyla that likely play important roles in the environment, and will assist in improving the reconstruction of the evolutionary history of Bacteria and Archaea. Moreover, these data will improve our ability to interpret metagenomics sequence data from diverse environments, which will be of tremendous value for microbial ecology and evolutionary studies to come.

Rinke, Christian; Sczyrba, Alex; Malfatti, Stephanie; Lee, Janye; Cheng, Jan-Fang; Stepanauskas, Ramunas; Eisen, Jonathan A.; Hallam, Steven; Inskeep, William P.; Hedlund, Brian P.; Sievert, Stefan M.; Liu, Wen-Tso; Tsiamis, George; Hugenholtz, Philip; Woyke, Tanja

2011-03-20T23:59:59.000Z

69

Microorganisms found in salt flats could offer new path to green hydrogen  

NLE Websites -- All DOE Office Websites (Extended Search)

"Proton pumps" are proteins that typically straddle a cellular membrane and transfer protons from inside the cell to the extracellular space. To view a larger version of the image, click on it. "Proton pumps" are proteins that typically straddle a cellular membrane and transfer protons from inside the cell to the extracellular space. To view a larger version of the image, click on it. "Proton pumps" are proteins that typically straddle a cellular membrane and transfer protons from inside the cell to the extracellular space. To view a larger version of the image, click on it. Microorganisms found in salt flats could offer new path to green hydrogen fuel July 16, 2013 Tweet EmailPrint ARGONNE, Ill. - A protein found in the membranes of ancient microorganisms that live in desert salt flats could offer a new way of using sunlight to generate environmentally friendly hydrogen fuel, according to a new study by researchers at the U.S. Department of

70

Live Working Resource Center  

Science Conference Proceedings (OSTI)

This report is a summary of work performed in 2008 on the EPRI Live Working Resource Center (LWRC) web site.

2008-12-16T23:59:59.000Z

71

Usable live programming  

Science Conference Proceedings (OSTI)

Programming today involves code editing mixed with bouts of debugging to get feedback on code execution. For programming to be more fluid, editing and debugging should occur concurrently as live programming. This paper describes how live execution ... Keywords: debugging, live programming

Sean McDirmid

2013-10-01T23:59:59.000Z

72

Live Working Resource Center  

Science Conference Proceedings (OSTI)

EPRI created the Live Working Resource Center web site to provide utilities the critical information they need to conduct live work safely and efficiently. This report is a summary of work performed in 2007 on the web site. See EPRI's Live Working Resource Center.

2007-11-30T23:59:59.000Z

73

Live Working Resource Forum  

Science Conference Proceedings (OSTI)

This project involves development of a Live Working Resource Forum for documenting common and unique live work practices. It also serves as a repository of lessons learned derived from analysis of live work incidents. This Technical Update report is the design document of the Forum.

2006-12-14T23:59:59.000Z

74

Living versus non-living criteria  

NLE Websites -- All DOE Office Websites (Extended Search)

Living versus non-living criteria Living versus non-living criteria Name: jconner Status: N/A Age: N/A Location: N/A Country: N/A Date: Around 1993 Question: What is the criteria for living versus non-living? Is life functions (growth, reproduction, etc.) sufficient or must structure also (cells) be included? Replies: Yes, textbooks are vague as no satisfactory answer has been found. This is probably because we have not tried very hard as life on earth is easy to recognize. As we begin to study outer space, we may need a better definition so we can recognize the very different forms of life that may exist elsewhere. All life forms on earth have deoxyribonucleic acids or ribonucleic acids. These molecules store then information necessary to build the next generation, so as to make more nucleic acid. One could ask whether organisms are merely the means to making more nucleic acids, in the form of genes. Many people have included the criterion of cellular structure in the definition of life but I have never known why. All life depends on other life forms for their reproduction, but viruses are particularly needy. Philosophers have taken some stabs at more general definitions of life, unlike most biologists who are usually satisfied to be able to recognize earth life when they see it. One definition I like is that living things are those that require energy to reproduce; and this reproduction is accomplished with some errors (ie. mutations). These errors distinguish life from inanimate crystals that can grow on themselves. This answer addresses how to define a living species, but ignore the questions of how to determine if any one organism is alive at any given time. That is a different question and one of degree

75

Engineering Biofuels from Photosynthetic Bacteria | Argonne National...  

NLE Websites -- All DOE Office Websites (Extended Search)

Engineering Biofuels from Photosynthetic Bacteria Technology available for licensing: Using photosynthetic bacteria to produce biofuels. 30-70% of the fuel's waste can be used to...

76

Geobiology of marine magnetotactic bacteria  

E-Print Network (OSTI)

Magnetotactic bacteria (MTB) biomineralize intracellular membrane-bound crystals of magnetite (Fe3O4) or greigite (Fe3S4), and are abundant in the suboxic to anoxic zones of stratified marine environments worldwide. Their ...

Simmons, Sheri Lynn

2006-01-01T23:59:59.000Z

77

Liquid Fuel from Heat-Loving Microorganisms: H2-Dependent Conversion of CO2 to Liquid Electrofuels by Extremely Thermophilic Archaea  

SciTech Connect

Electrofuels Project: NC State is working with the University of Georgia to create Electrofuels from primitive organisms called extremophiles that evolved before photosynthetic organisms and live in extreme, hot water environments with temperatures ranging from 167-212 degrees Fahrenheit The team is genetically engineering these microorganisms so they can use hydrogen to turn carbon dioxide directly into alcohol-based fuels. High temperatures are required to distill the biofuels from the water where the organisms live, but the heat-tolerant organisms will continue to thrive even as the biofuels are being distilledmaking the fuel-production process more efficient. The microorganisms dont require light, so they can be grown anywhereinside a dark reactor or even in an underground facility.

2010-07-01T23:59:59.000Z

78

EPRI Live Working Project  

Science Conference Proceedings (OSTI)

The Electric Power Research Institute (EPRI) has been conducting research in the area of live working for several decades. This research has resulted in a large number of reports and other products. To help users locate the results of EPRIs research in live working, an annual update is prepared containing brief descriptions of the products.BackgroundLive workthe performance of maintenance, construction, or testing on equipment and circuits that ...

2012-09-06T23:59:59.000Z

79

Fully deuterated microorganisms: Tools in magnetic resonance and neutron scattering  

SciTech Connect

Current work at Argonne emphasizes the use of fully deuterated algae and cyanobacteria as tools in the study of photosynthesis and as a source of complex substrates for the culture of engineered overproducing bacteria. 17 refs., 1 fig., 1 tab.

Crespi, H.L.

1988-01-01T23:59:59.000Z

80

Why Sequence Biogeochemically Important Bacteria?  

NLE Websites -- All DOE Office Websites (Extended Search)

Biogeochemically Important Bacteria? Biogeochemically Important Bacteria? DOE-JGI will be sequencing three biogeochemically important bacteria, Diaphorobacter sp. strain TPSY, Ferrutens nitratireducens strain 2002 and Azospira suillum strain PS. These organisms represent diverse genera capable of anaerobically oxidizing both iron(II) and humic acids by using nitrate as the electron acceptor. Two of these organisms, strain 2002 and strain TPSY, are also capable of the anaerobic nitrate-dependent oxidation of uranium(IV) to uranium(VI). Left to right, Azospira suillum PS, Ferrutens nitratireducens 2002, and Diaphorobacter TPSY. Nitrate-dependent microbial metal oxidation is of critical importance because of its potential effect on the fate and transport of radioactive contaminants. Nitrate-dependent Iron(II) oxidation by organisms such as

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

Aerobic microorganism for the degradation of chlorinated aliphatic hydrocarbons  

DOE Patents (OSTI)

A chlorinated aliphatic hydrocarbon-degrading microorganism, having American Type Culture Collection accession numbers ATCC 53570 and 53571, in a biologically pure culture aseptically collected from a deep subsurface habitat and enhanced, mineralizes trichloroethylene and tetrachloroethylene to HCl, H.sub.2 O and Co.sub.2 under aerobic conditions stimulated by methane, acetate, methanol, tryptone-yeast extract, propane and propane-methane.

Fliermans, Carl B. (Augusta, GA)

1989-01-01T23:59:59.000Z

82

Aerobic microorganism for the degradation of chlorinated aliphatic hydrocarbons  

DOE Patents (OSTI)

This invention pertains to a chlorinated aliphatic hydrocarbon-degrading microorganism, having American Type Culture Collection accession numbers ATCC 53570 and 53571, in a biologically pure culture aseptically collected from a deep subsurface habitat and enhanced, mineralizes trichloroethylene and tetrachloroethylene to HCl, H{sub 2}O and CO{sub 2} under aerobic conditions stimulated by methane, acetate, methanol, tryptone-yeast extract, propane and propane-methane.

Fliermans, C.B.

1988-10-12T23:59:59.000Z

83

ASHRAE's Living Laboratory  

SciTech Connect

ASHRAE recently remodeled its headquarters building in Atlanta with the intention of making the building a LEED Gold building. As part of that renovation the building was enhanced with additional sensors and monitoring equipment to allow it to serve as a Living Laboratory for use by members and the general public to study the detailed energy use and performance of buildings. This article provides an overview of the Living Laboratory and its capabilities.

Jarnagin, Ronald E.; Brambley, Michael R.

2008-10-01T23:59:59.000Z

84

Why Sequence Cellulose Degrading Bacteria?  

NLE Websites -- All DOE Office Websites (Extended Search)

Cellulose Degrading Bacteria? Cellulose Degrading Bacteria? One of the major DOE missions is the production of renewable fuels to reduce our dependence on foreign oil, and also to take the place of petroleum-based fuels as these resources dwindle. Biologically produced ethanol is one possible replacement for fossil fuels. Currently, ethanol is produced from corn starch, but there is much research into using lignocellulosic materials (those containing cellulose, hemicellulose, and lignin) as the raw material for ethanol production. Ethanol production from cellulose requires several steps: pretreatment with steam, acid, or ammonia; digestion of cellulose to sugars; and fermentation of sugars to ethanol. The slowest and most expensive step is the breakdown of cellulose, chemically accomplished by cellulases. The second and third

85

Live From Outer Space  

NLE Websites -- All DOE Office Websites (Extended Search)

Live from Outer Space: How Cells Influence the Growth of Nanostructures Live from Outer Space: How Cells Influence the Growth of Nanostructures Far above the heads of Earthlings, arrays of single-cell creatures embedded in nanostructures ride on the International Space Station (courtesy of Sandia National Laboratories and the University of New Mexico, NASA, and the U.S. Air Force) to test whether nanostructures whose formations were directed by yeast and other single cells can create more secure homes for their occupants-even in the vacuum and radiation of outer space-than those created by more standard chemical procedures. Cheap, tiny, and very lightweight sensors of chemical or biological agents could be made from long-lived cells that require no upkeep, yet sense and then communicate effectively with each other and their external

86

Effects of remediation amendments on vadose zone microorganisms  

SciTech Connect

Surfactant-based foam delivery technology has been studied to remediate Hanford 200 area deep vadose zone sediment. However, the surfactants and remediation amendments have an unknown effect on indigenous subsurface microorganisms. Microbial populations are important factors to consider in remediation efforts due to their potential to alter soil geochemistry. This project focuses on measuring microbial metabolic responses to remediation amendments in batch and column studies using Deep Vadose Zone Sediments. Initial studies of the microbes from Hanford 200 area deep vadose zone sediment showed surfactants sodium dodecyl sulfate (SDS) and cocamidopropyl betaine (CAPB) and remediation amendment calcium polysulfide (CPS) had no affect on microbial growth using BiologTM Ecoplates. To move towards a more realistic field analog, soil columns were packed with Hanford 200 Area sediment. Once microbial growth in the column was verified by observing growth of the effluent solution on tryptic soy agar plates, remedial surfactants were injected into the columns, and the resulting metabolic diversity was measured. Results suggest surfactant sodium dodecyl sulfate (SDS) stimulates microbial growth. The soil columns were also visualized using X-ray microtomography to inspect soil packing and possibly probe for evidence of biofilms. Overall, BiologTM Ecoplates provide a rapid assay to predict effects of remediation amendments on Hanford 200 area deep vadose zone microorganisms.

Miller, Hannah M.; Tilton, Fred A.

2012-08-10T23:59:59.000Z

87

Growth, metabolic partitioning, and the size of microorganisms  

E-Print Network (OSTI)

Population growth rate is a fundamental ecological and evolutionary characteristic of living organisms, but individuals must balance the metabolism devoted to biosynthesis and reproduction against the maintenance of existing ...

Dutkiewicz, Stephanie

88

NEWTON: Bacteria Survival in the Stomach  

NLE Websites -- All DOE Office Websites (Extended Search)

Bacteria Survival in the Stomach Bacteria Survival in the Stomach Name: Lianne Status: other Grade: other Location: Outside U.S. Country: USA Date: Summer 2013 Question: Is H. Pylorii the only bacteria able to survive the acidic condition of the stomach, or are there others? Replies: Hi Lianne, Thanks for the question. Yes, other bacteria are able to survive the acidic conditions in the stomach. For instance, the bacteria that are present in acidopholus yougurt (and milk) are able to survive the stomach's acid and to repopulate the large intestine. These acidopholus bacteria are "good" bacteria and are useful in restoring bacterial colonies in the large intestine after a treatment of antibiotics. I hope this helps. Please let me know if you have more questions. Thanks Jeff I don't know, but I would be very surprised given the number of bacterial species, that H. Pylori is uniquely adapted to low pH environments.

89

Formation of hydrocarbons by bacteria and algae  

SciTech Connect

A literature review has been performed summarizing studies on hydrocarbon synthesis by microorganisms. Certain algal and bacterial species produce hydrocarbons in large quantities, 70 to 80% of dry cell mass, when in a controlled environment. The nutritional requirements of these organisms are simple: CO/sub 2/ and mineral salts. The studies were initiated to determine whether or not microorganisms played a role in petroleum formation. 90 references. (DMC)

Tornabene, T.G.

1980-12-01T23:59:59.000Z

90

Tiny Conspiracies: cell-to-cell communication in bacteria  

Science Conference Proceedings (OSTI)

Tiny Conspiracies: cell-to-cell communication in bacteria. Purpose: Bacteria, primitive single-celled organisms, communicate ...

2012-11-13T23:59:59.000Z

91

Ropes for Live Working  

Science Conference Proceedings (OSTI)

Fiber ropes represent an essential tool in many applications associated with the electric power industry. Ropes used in proximity to or in contact with high-voltage power lines require demanding dielectric properties as well as strength and durability. A comprehensive understanding of live working (LW) rope construction, performance, and use is necessary for safe and efficient operations wherever such ropes are used.

2008-11-18T23:59:59.000Z

92

Living Systems Energy Module  

DOE Green Energy (OSTI)

The Living Systems Energy Module, renamed Voyage from the Sun, is a twenty-lesson curriculum designed to introduce students to the major ways in which energy is important in living systems. Voyage from the Sun tells the story of energy, describing its solar origins, how it is incorporated into living terrestrial systems through photosynthesis, how it flows from plants to herbivorous animals, and from herbivores to carnivores. A significant part of the unit is devoted to examining how humans use energy, and how human impact on natural habitats affects ecosystems. As students proceed through the unit, they read chapters of Voyage from the Sun, a comic book that describes the flow of energy in story form (Appendix A). During the course of the unit, an ``Energy Pyramid`` is erected in the classroom. This three-dimensional structure serves as a classroom exhibit, reminding students daily of the importance of energy and of the fragile nature of our living planet. Interactive activities teach students about adaptations that allow plants and animals to acquire, to use and to conserve energy. A complete list of curricular materials and copies of all activity sheets appear in Appendix B.

NONE

1995-09-26T23:59:59.000Z

93

Influence of gamma irradiation on the metabolic activity of sulfate-reducing bacteria  

SciTech Connect

When water is pumped into oil-bearing seams to increase oil production, the microorganisms in the injected water fall into favorable ecological conditions and, quickly adapting, form a biocenosis and begin to actively develop. Among the anaerobic microorganisms, the most hazardous from the corrosion viewpoint are the sulfate-reducing bacteria (SRB), which are the main producers of hydrogen sulfide as the product of anaerobic respiration. This paper reports on the effect of gamma rays on the metabolic study of SRB Desulfovibrio desulfuricans in the nutrient medium Postgate B. The radioactive source used is a /sup 60/CoK-125 unit with a power of 700 rad/sec. The required dose of gamma rays was calculated from the exposure times of samples with the test medium in the radiation zone o the isotope /sup 60/Co. The criterion characterizing the effectiveness of suppression of development of the bacteria is the concentration of biogenic hydrogen sulfide produced, as determined by iodometric titration.

Agaev, N.M.; Guseinov, M.M.; Smorodin, A.E.

1985-09-01T23:59:59.000Z

94

Technical Note: Evaluation of Effective Microorganisms (EM) In Solid Waste Management  

E-Print Network (OSTI)

produces a high quality compost, which contributes towardorganic matters, compost, effective microorganisms (EM). Athis point, the finished compost was collected and sieved.

Sekeran, V.; Balaji, C.; Bhagavathipushpa, T.

2005-01-01T23:59:59.000Z

95

Why Sequence Bacteria That Reduce Sulfur Compounds?  

NLE Websites -- All DOE Office Websites (Extended Search)

Bacteria That Reduce Sulfur Compounds? Combustion of sulfur-containing fuels, such as coal, oil, and natural gas, contributes significantly to global environmental problems, such...

96

Spectroscopic diagnostics for bacteria in biologic sample  

DOE Patents (OSTI)

A method to analyze and diagnose specific bacteria in a biologic sample using spectroscopy is disclosed. The method includes obtaining the spectra of a biologic sample of a non-infected patient for use as a reference, subtracting the reference from the spectra of an infected sample, and comparing the fingerprint regions of the resulting differential spectrum with reference spectra of bacteria in saline. Using this diagnostic technique, specific bacteria can be identified sooner and without culturing, bacteria-specific antibiotics can be prescribed sooner, resulting in decreased likelihood of antibiotic resistance and an overall reduction of medical costs.

El-Sayed, Mostafa A. (Atlanta, GA); El-Sayed, Ivan H. (Somerville, MA)

2002-01-01T23:59:59.000Z

97

QUANTATITIVE PCR ASSAY FOR MARINE BACTERIA  

E-Print Network (OSTI)

COMPLETION REPORT QUANTATITIVE PCR ASSAY FOR MARINE BACTERIApolymerase chain reaction (PCR) amplification of their smallIdentification of bacteria by PCR amplification is specific

Brunk, Clifford F.

2003-01-01T23:59:59.000Z

98

Engineering and Coordination of Regulatory Networks and Intracellular Complexes to Maximize Hydrogen Production by Phototrophic Microorganisms  

DOE Green Energy (OSTI)

This project is a collaboration with F. R. Tabita of Ohio State. Our major goal is to understand the factors and regulatory mechanisms that influence hydrogen production. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Our part of the project was to develop a modeling technique to investigate the metabolic network in connection to hydrogen production and regulation. Organisms must balance the pathways that generate and consume reducing power in order to maintain redox homeostasis to achieve growth. Maintaining this homeostasis in the nonsulfur purple photosynthetic bacteria is a complex feat with many avenues that can lead to balance, as these organisms possess versatile metabolic capabilities including anoxygenic photosynthesis, aerobic or anaerobic respiration, and fermentation. Growth is achieved by using H{sub 2} as an electron donor and CO{sub 2} as a carbon source during photoautotrophic and chemoautotrophic growth, where CO{sub 2} is fixed via the Calvin-Benson-Bassham (CBB) cycle. Photoheterotrophic growth can also occur when alternative organic carbon compounds are utilized as both the carbon source and electron donor. Regardless of the growth mode, excess reducing equivalents generated as a result of oxidative processes, must be transferred to terminal electron acceptors, thus insuring that redox homeostasis is maintained in the cell. Possible terminal acceptors include O{sub 2}, CO{sub 2}, organic carbon, or various oxyanions. Cells possess regulatory mechanisms to balance the activity of the pathways which supply energy, such as photosynthesis, and those that consume energy, such as CO{sub 2} assimilation or N{sub 2} fixation. The major route for CO{sub 2} assimilation is the CBB reductive pentose phosphate pathway, whose key enzyme is ribulose 1,5-biphosphate carboxylase/oxygenase (RubisCO). In addition to providing virtually all cellular carbon during autotrophic metabolism, RubisCO-mediated CO{sub 2} assimilation is also very important for nonsulfur purple photosynthetic bacteria under photoheterotrophic growth conditions since CO{sub 2} becomes the major electron sink under these conditions. In this work, Ensemble Modeling (EM) was developed to examine the behavior of CBB-compromised RubisCO knockout mutant strains of the nonsulfur purple photosynthetic bacterium Rhodobacter sphaeroides. Mathematical models of metabolism can be a great aid in studying the effects of large perturbations to the system, such as the inactivation of RubisCO. Due to the complex and highly-interconnected nature of these networks, it is not a trivial process to understand what the effect of perturbations to the metabolic network will be, or vice versa, what enzymatic perturbations are necessary to yield a desired effect. Flux distribution is controlled by multiple enzymes in the network, often indirectly linked to the pathways of interest. Further, depending on the state of the cell and the environmental conditions, the effect of a perturbation may center around how it effects the carbon flow in the network, the balancing of cofactors, or both. Thus, it is desirable to develop mathematical models to describe, understand, and predict network behavior. Through the development of such models, one may gain the ability to generate a set of testable hypotheses for system behavior.

James C. Liao

2012-05-22T23:59:59.000Z

99

Improved hydrogen photoproduction from photosynthetic bacteria and green algae  

DOE Green Energy (OSTI)

Photosynthetic bacteria evolve hydrogen at much higher rates than do other classes of photosynthetic microorganisms. In addition, they tolerate harsh environments, grow rapidly, and utilize both visible and near infrared light in photosynthesis. They do not split water, but this does not necessarily eliminate their potential use in future applied systems. They are easily manipulated genetically, and thus might be modified to metabolize common biomass waste materials in place of expensive defined organic substrates. Furthermore, the potential for increasing hydrogen photoproduction via genetic techniques is promising. Strains that partially degrade cellulose, have high photoproduction rates, or contain very large amounts of the enzymes associated with hydrogen metabolism have been isolated. Green algae also produce hydrogen but are capable of using water as a substrate. For example, C. reinhardi can evolve hydrogen and oxygen at a molar ratio approaching 2:1. Based upon effect of dichlorophenyl dimethylurea (a specific inhibitor of photosystem II, PSII) on hydrogen photoproduction in the wild type strain and upon results obtained with PSII mutants, one can demonstrate that water is the major source of electrons for hydrogen production. The potential efficiency of in vivo coupling between hydrogenase and the photosynthetic electron transport system is high. Up to 76% of the reductants generated by the electron transport system can be channeled directly to the enzyme for in vivo hydrogen production. Rates exceeding 170 ..mu..moles of H/sub 2/ mg Chl/sup -1/ hr/sup -1/ have been observed.

Weaver, P.F.; Lien, S.; Seibert, M.

1979-01-01T23:59:59.000Z

100

Method of dispersing a hydrocarbon using bacteria  

DOE Patents (OSTI)

New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

Tyndall, Richard L. (Clinton, TN)

1996-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


101

Hydrogen metabolism of photosynthetic bacteria and algae  

Science Conference Proceedings (OSTI)

The metabolism, metabolic pathways and biochemistry of hydrogen in photosynthetic bacteria and algae are reviewed. Detailed information on the occurrence and measurement of hydrogenase activity is presented. Hydrogen production rates for different species of algae and bacteria are presented. 173 references, 1 figure, 7 tables.

Kumazawa, S.; Mitsui, A.

1982-01-01T23:59:59.000Z

102

Living with Electric Vehicles  

NLE Websites -- All DOE Office Websites (Extended Search)

Living with Electric Vehicles Living with Electric Vehicles JOHN DAVIS: On any given weekend, somewhere you'll find a gathering of cars and a group of enthusiasts assembled around them. Be the hotrods classics or sports cars, each genre of the car's evolution has developed loyal following. And electric cars are no exception. The recent National Plug-in day included events held at hundreds of sites across the U.S. enticing EV aficionados to check out the latest models and share their passion for gas-free motoring. JOHN BARRACCA: The dealer gives you 9.3 gallons. I haven't used all of that yet. But, when I get 3 gallons low, I put 3 gallons in. So, I'm still at almost a full tank. The last time I put 3 gallons in was February and this is September 23rd. JOHN DAVIS: All of the owners we talked with were pleased with their plug-in car's fuel

103

Chemotactic selection of pollutant degrading soil bacteria  

DOE Patents (OSTI)

A method is described for identifying soil microbial strains which may be bacterial degraders of pollutants. This method includes: Placing a concentration of a pollutant in a substantially closed container; placing the container in a sample of soil for a period of time ranging from one minute to several hours; retrieving the container and collecting its contents; microscopically determining the identity of the bacteria present. Different concentrations of the pollutant can be used to determine which bacteria respond to each concentration. The method can be used for characterizing a polluted site or for looking for naturally occurring biological degraders of the pollutant. Then bacteria identified as degraders of the pollutant and as chemotactically attracted to the pollutant are used to innoculate contaminated soil. To enhance the effect of the bacteria on the pollutant, nutrients are cyclicly provided to the bacteria then withheld to alternately build up the size of the bacterial colony or community and then allow it to degrade the pollutant.

Hazen, T.C.

1991-03-04T23:59:59.000Z

104

Scientists Launch the Genomic Encyclopedia of Bacteria and Archaea | U.S.  

NLE Websites -- All DOE Office Websites (Extended Search)

Scientists Scientists Launch the Genomic Encyclopedia of Bacteria and Archaea News Featured Articles 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 Science Headlines Presentations & Testimony News Archives Contact Information Office of Science U.S. Department of Energy 1000 Independence Ave., SW Washington, DC 20585 P: (202) 586-5430 12.28.09 Scientists Launch the Genomic Encyclopedia of Bacteria and Archaea Unlocking the diversity of microbial communities may benefit biofuel production, global carbon storage, and bioremediation. Print Text Size: A A A Subscribe FeedbackShare Page Scientists estimate that there are approximately 4 × 10^30 microbes living on the planet. To put this number into perspective, there are 4,000,000,000,000,000,000,000,000,000,000 microbes living on the planet

105

Shared lives, shared energy  

Science Conference Proceedings (OSTI)

A social experiment in Denmark is described in which 25 families combine private ownership (each family owns its own home) and collectivism (each family owns 1/25 of the grounds, large common house and other facilities). The superinsulated individual homes are small (solar, wind and/or oil-fired boiler. Adequate hot water storage is provided using solar collectors and a 55 kW Vesta wind generator (surplus power is sold). All south facing roof surfaces are fitted with solar collectors (4455 ft/sup 2/ total). A total of 70% of the energy used is produced on site (solar and wind). The manner of living and sharing (child care, automobiles, cooking, etc.) is described as well as typical floor plans for the units. Other collective housing in Denmark is described and it is postulated that overdrevet may serve as a model. (MJJ)

Madsen, P.; Goss, K.

1982-07-01T23:59:59.000Z

106

Reduction and Immobilization of Radionuclides and Toxic Metal Ions Using Combined Zero Valent Iron and Anaerobic Bacteria  

Science Conference Proceedings (OSTI)

The use of zero valent iron, permeable reactive barriers (PRBs) for groundwater remediation continues to increase. AN exciting variation of this technology involves introducing anaerobic bacteria into these barriers so that both biological and abiotic pollutant removal processes are functional. This work evaluated the hypothesis that a system combining a mixed culture of sulfate reducing bacteria (SRB) with zero valent iron would have a greater cr(VI) removal efficiency and a greater total Cr(VI) removal capacity than a zero valent iron system without the microorganisms. Hence, the overall goal of this research was to compare the performance of these types of systems with regard to their Cr(VI) removal efficiency and total Cr(VI) removal capacity. Both batch and continuous flow reactor systems were evaluated.

Lenly J. Weathers; Lynn E. Katz

2002-05-29T23:59:59.000Z

107

Why sequence Bacteria from Lake Washington?  

NLE Websites -- All DOE Office Websites (Extended Search)

bacteria from Lake Washington? bacteria from Lake Washington? Previous collaborations between the University of Washington team and the DOE JGI involving both single genome and metagenomic sequencing have greatly enhanced the community's ability to explore the diversity of bacteria functionally active in metabolism of single carbon compounds, known as methylotrophs, isolated from Lake Washington (Seattle, Washington) sediment. Sequencing genomes of 50 methylotroph isolates from the Lake Washington will further enhance the methylotroph community knowledge database providing a much higher level of resolution of global (meta)transcriptomic and (meta)proteomic analyses, as well as species interaction studies, informing a better understanding of biogeochemical cycling of carbon and nitrogen.

108

Motility fractionation of bacteria by centrifugation  

E-Print Network (OSTI)

Centrifugation is a widespread laboratory technique used to separate mixtures into fractions characterized by a specific size, weight or density. We demonstrate that centrifugation can be also used to separate swimming cells having different motility. To do this we study self-propelled bacteria under the influence of an external centrifugal field. Using dynamic image correlation spectroscopy we measure the spatially resolved motility of bacteria after centrifugation. A significant gradient in swimming-speeds is observed for increasing centrifugal speeds. Our results can be reproduced by a model that treats bacteria as "hot" colloidal particles having a diffusion coefficient that depends on the swimming speed.

Claudio Maggi; Alessia Lepore; Jacopo Solari; Alessandro Rizzo; Roberto Di Leonardo

2013-10-10T23:59:59.000Z

109

Advanced Biofuels: How Scientists are Engineering Bacteria to...  

NLE Websites -- All DOE Office Websites (Extended Search)

Advanced Biofuels: How Scientists are Engineering Bacteria to Help Drive America Advanced Biofuels: How Scientists are Engineering Bacteria to Help Drive America December 6, 2011 -...

110

Why Sequence Bacteria in Deep Sea Hydrothermal Vents?  

NLE Websites -- All DOE Office Websites (Extended Search)

Bacteria in Deep Sea Hydrothermal Vents? The project focuses on using single-cell genomics to sequence nearly a dozen genomes of uncultivated bacteria that are found in...

111

Live Work Guide for Substations  

Science Conference Proceedings (OSTI)

Live work (work on energized circuits) is the preferred method of maintenance where system integrity, system reliability, and operating revenues are at a premium and removal of a circuit or a substation from service is not acceptable. Live work (LW) may also be beneficial in construction and storm damage repair. Furthermore, live work may be unavoidable in the case of substations that serve essential facilities such as hospitals, law enforcement, fire departments, and intrusion alarms if standby local ge...

2004-10-28T23:59:59.000Z

112

Robotic Technologies for Live Working  

Science Conference Proceedings (OSTI)

The objective of this report is to review selected available robotic technologies and evaluate their possible applicability to live working. The selection criteria for robotic technologies included suitability for operation in a high-voltage environment; mechanical load capacity; and extent of reach, size, and weight compatible with live work applications. The report provides a brief history of robotics in live work dating back to late 1970s and the development of the EPRI TOMCAT (Teleoperator for Operat...

2010-12-23T23:59:59.000Z

113

Why Sequence Sulfur-Oxidizing Bacteria?  

NLE Websites -- All DOE Office Websites (Extended Search)

Sulfur-Oxidizing Bacteria? Sulfur-Oxidizing Bacteria? Several environmental problems, such as acid rain, biocorrosion, etc., are caused by sulfur compounds, such as sulfur dioxide (SO2) and hydrogen sulfide (H2S). A sustainable process to remove these sulfur compounds is the production of elemental sulfur from H2S-containing gas streams by the use of sulfide-oxidizing bacteria. In this process, H2S is absorbed into the alkaline solution in the scrubber unit, followed by the biological oxidation of H2S to elemental sulfur and the recycling of water. With this two-step process, a variety of gas streams (i.e., natural gas, synthesis gas, biogas, and refinery gas) can be treated. For the treatment of sulfate-containing waste streams, an extra step has to be introduced: the transformation of sulfate into H2S by sulfate-reducing bacteria. In

114

Probiotic Bacteria Induce a Glow of Health  

E-Print Network (OSTI)

Radiant skin and hair are universally recognized as indications of good health. However, this glow of health display remains poorly understood. We found that feeding of probiotic bacteria to aged mice induced integumentary ...

Levkovich, Tatiana

115

Fuel from Bacteria: Bioconversion of Carbon Dioxide to Biofuels by Facultatively Autotrophic Hydrogen Bacteria  

Science Conference Proceedings (OSTI)

Electrofuels Project: Ohio State is genetically modifying bacteria to efficiently convert carbon dioxide directly into butanol, an alcohol that can be used directly as a fuel blend or converted to a hydrocarbon, which closely resembles a gasoline. Bacteria are typically capable of producing a certain amount of butanol before it becomes too toxic for the bacteria to survive. Ohio State is engineering a new strain of the bacteria that could produce up to 50% more butanol before it becomes too toxic for the bacteria to survive. Finding a way to produce more butanol more efficiently would significantly cut down on biofuel production costs and help make butanol cost competitive with gasoline. Ohio State is also engineering large tanks, or bioreactors, to grow the biofuel-producing bacteria in, and they are developing ways to efficiently recover biofuel from the tanks.

None

2010-07-01T23:59:59.000Z

116

Utilization of Vinegar for Isolation of Cellulose Producing Acetic Acid Bacteria  

Science Conference Proceedings (OSTI)

Wastes of traditionally fermented Turkish vinegar were used in the isolation of cellulose producing acetic acid bacteria. Waste material was pre-enriched in Hestrin-Schramm medium and microorganisms were isolated by plating dilution series on HS agar plates The isolated strains were subjected to elaborate biochemical and physiological tests for identification. Test results were compared to those of reference strains Gluconacetobacter xylinus DSM 46604, Gluconacetobacter hansenii DSM 5602 and Gluconacetobacter liquefaciens DSM 5603. Seventeen strains, out of which only three were found to secrete the exopolysaccharide cellulose. The highest cellulose yield was recorded as 0.263+-0.02 g cellulose L{sup -1} for the strain AS14 which resembled Gluconacetobacter hansenii in terms of biochemical tests.

Aydin, Y. Andelib; Aksoy, Nuran Deveci [Chemical Engineering Department of Istanbul Technical University, Ayazaga, Maslak, Istanbul, 34469 (Turkey)

2010-06-17T23:59:59.000Z

117

Mtr Extracellular Electron Transfer Pathways in Fe(III)-reducing or Fe(II)-oxidizing Bacteria: A Genomic Perspective  

Science Conference Proceedings (OSTI)

Originally discovered in the dissimilatory metal-reducing bacterium Shewanella oneidensis MR-1 (MR-1), the Mtr (i.e., metal-reducing) pathway exists in all characterized strains of metal-reducing Shewanella. The protein components identified to date for the Mtr pathway of MR-1 include four multi-heme c-type cytochromes (c-Cyts), CymA, MtrA, MtrC and OmcA, and a porin-like, outer membrane protein MtrB. They are strategically positioned along the width of the MR-1 cell envelope to mediate electron transfer from the quinone/quinol pool in the inner-membrane to the Fe(III)-containing minerals external to the bacterial cells. A survey of microbial genomes revealed homologues of the Mtr pathway in other dissimilatory Fe(III)-reducing bacteria, including Aeromonas hydrophila, Ferrimonas balearica and Rhodoferax ferrireducens, and in the Fe(II)-oxidizing bacteria Dechloromonas aromatica RCB, Gallionella capsiferriformans ES-2 and Sideroxydans lithotrophicus ES-1. The widespread distribution of Mtr pathways in Fe(III)-reducing or Fe(II)-oxidizing bacteria emphasizes the importance of this type of extracellular electron transfer pathway in microbial redox transformation of Fe. Their distribution in these two different functional groups of bacteria also emphasizes the bi-directional nature of electron transfer reactions carried out by the Mtr pathways. The characteristics of the Mtr pathways may be shared by other pathways used by microorganisms for exchanging electrons with their extracellular environments.

Shi, Liang; Rosso, Kevin M.; Zachara, John M.; Fredrickson, Jim K.

2012-12-01T23:59:59.000Z

118

Quantification of syntrophic fatty acid-{beta}-oxidizing bacteria in a mesophilic biogas reactor by oligonucleotide probe hybridization  

Science Conference Proceedings (OSTI)

Small-subunit rRNA sequences were obtained for two saturated fatty acid-{beta}-oxidizing syntrophic bacteria, Syntrophomonas sapovorans and Syntrophomonas wolfei LYB, and sequence analysis confirmed their classification as members of the family Syntrophomonadaceae. S.wolfei LYB was closely related to S.wolfei subsp. solfei, but S. sapovorans did not cluster with the other members of the genus Syntrophomonas. Five oligonucleotide probes targeting the small-subunit rRNA of different groups within the family Syntrophomonadaceae, which contains all currently known saturated fatty acid-{beta}-oxidizing syntrophic bacteria, were developed and characterized. The probes were designed to be specific at the family, genus, and species levels and were characterized by temperature-of-dissociation and specificity studies. To demonstrate the usefulness of the probes for the detection and quantification of saturated fatty acid-{beta}-oxidizing syntrophic bacteria in methanogenic environments, the microbial community structure of a sample from a full-scale biogas plant was determined. Hybridization results with probes for syntrophic bacteria and methanogens were compared to specific methanogenic activities and microbial numbers determined with most-probable-number estimates. Most of the methanogenic rRNA was comprised of Methanomicrobiales rRNA, suggesting that members of this order served as the main hydrogen-utilizing microorganisms. Between 0.2 and 1% of the rRNA was attributed to the Syntrophomonadaceae, or which the majority was accounted for by the genus Syntrophomonas.

Hansen, K.H.; Ahring, B.K.; Raskin, L.

1999-11-01T23:59:59.000Z

119

Determination of kinetic coefficients for the simultaneous reduction of sulfate and uranium by Desulfovibrio desulfuricans bacteria  

Science Conference Proceedings (OSTI)

Uranium contamination of groundwaters and surface waters near abandoned mill tailings piles is a serious concern in many areas of the western United States. Uranium usually exists in either the U(IV) or the U(VI) oxidation state. U(VI) is soluble in water and, as a result, is very mobile in the environment. U(IV), however, is generally insoluble in water and, therefore, is not subject to aqueous transport. In recent years, researchers have discovered that certain anaerobic microorganisms, such as the sulfate-reducing bacteria Desulfovibrio desulfuricans, can mediate the reduction of U(VI) to U(IV). Although the ability of this microorganism to reduce U(VI) has been studied in some detail by previous researchers, the kinetics of the reactions have not been characterized. The purpose of this research was to perform kinetic studies on Desulfovibrio desulficans bacteria during simultaneous reduction of sulfate and uranium and to determine the phase in which uranium exists after it has been reduced and precipitated from solution. The studies were conducted in a laboratory-scale chemostat under substrate-limited growth conditions with pyruvate as the substrate. Kinetic coefficients for substrate utilization and cell growth were calculated using the Monod equation. The maximum rate of substrate utilization (k) was determined to be 4.70 days{sup {minus}1} while the half-velocity constant (K{sub s}) was 140 mg/l COD. The yield coefficient (Y) was determined to be 0.17 mg cells/mg COD while the endogenous decay coefficient (k{sub d}) was calculated as 0.072 days{sup {minus}1}. After reduction, U(IV) Precipitated from solution in the uraninite (UO{sub 2}) phase. Uranium removal efficiency as high as 90% was achieved in the chemostat.

Tucker, M.D.

1995-05-01T23:59:59.000Z

120

Live visuals tutorial: part III  

Science Conference Proceedings (OSTI)

In this part of the course, we give a tutorial on how to compose live visuals. First, we talk about hardware and software setup needed for a live visual performance. Then we present concepts to prepare and organize huge media libraries allowing for instant ...

Pascal Mueller

2007-08-01T23:59:59.000Z

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


121

Why Sequence Freshwater Iron-Oxidizing Bacteria?  

NLE Websites -- All DOE Office Websites (Extended Search)

Freshwater Iron-Oxidizing Bacteria? Freshwater Iron-Oxidizing Bacteria? The goal of this project is to obtain complete genome sequences for six different freshwater iron (Fe)-oxidizing bacteria (FeOB). Four of these are oxygen-dependent iron-oxidizing β-proteobacteria, and three of these, Sideroxydans lithotrophicus, Gallionella capsiferriformans, and strain TW-2, are capable of chemolithoautotrophic growth (that is, obtaining energy by the oxidation of inorganic compounds) using Fe(II) as sole energy source under microaerobic (low-oxygen) conditions. The fourth organism, Leptothrix cholodnii, is a sheath-forming heterotrophic (i.e., using complex organic compounds for nutrition) organism that oxidizes both Fe(II) and Mn(II) and deposits a ferromanganic coating on its sheath. In addition,

122

New microorganisms and processes for MEOR. Quarterly report ending September 30, 1992  

SciTech Connect

Oil reservoirs naturally contain inorganic and organic materials which can be exploited through simple supplementation to support the growth of microorganisms which aid in the release of oil from the rock matrix. Other compounds which may serve as nutritional sources for microorganisms are added to reservoirs during production and operation of oil fields. These materials include sulfate, nitrate, carbonate, volatile fatty acids, nitrogen-containing corrosion inhibitors, phosphorous-containing scale inhibitors and trace elements. Our experiments show that, with minimal supplementation, growth of naturally-occurring microorganisms can be used to produce viscosifying agents to aid oil recovery. This natural microflora is also capable of removing sulfide from oil reservoirs and preventing the formation of new sulfide leading to both more oil recovery and increased value of the produced oil. The metabolic products of these microorganisms are Co{sub 2}, water, N{sub 2} and oxidized forms of sulfur, all of which are environmentally innocuous. Laboratory experiments with both defined mixtures of microorganisms as well as mixed populations both release more oil from sand pack columns.

Sperl, G.T.; Sperl, P.L.

1992-12-31T23:59:59.000Z

123

Blood Banking in Living Droplets  

E-Print Network (OSTI)

Blood banking has a broad public health impact influencing millions of lives daily. It could potentially benefit from emerging biopreservation technologies. However, although vitrification has shown advantages over traditional ...

Samot, Josh

124

Biofuel from Bacteria and Sunlight: Shewanella as an Ideal Platform for Producing Hydrocarbons  

SciTech Connect

Broad Funding Opportunity Announcement Project: The University of Minnesota is developing clean-burning, liquid hydrocarbon fuels from bacteria. The University is finding ways to continuously harvest hydrocarbons from a type of bacteria called Shewanella by using a photosynthetic organism to constantly feed Shewanella the sugar it needs for energy and hydrocarbon production. The two organisms live and work together as a system. Using Shewanella to produce hydrocarbon fuels offers several advantages over traditional biofuel production methods. First, it eliminates many of the time-consuming and costly steps involved in growing plants and harvesting biomass. Second, hydrocarbon biofuels resemble current petroleum-based fuels and would therefore require few changes to the existing fuel refining and distribution infrastructure in the U.S.

None

2010-01-01T23:59:59.000Z

125

Human Occupancy as a Source of Indoor Airborne Bacteria  

E-Print Network (OSTI)

Exposure to specific airborne bacteria indoors is linked to infectious and noninfectious adverse health outcomes. However, the sources and origins of bacteria suspended in indoor air are not well understood. This study ...

Hospodsky, Denina

126

The Influence of the Mushroom Compost Application on the Microorganism Quantity of Reclamated Soil  

Science Conference Proceedings (OSTI)

The mushroom compost which was produced from farm can be used to improving the quality of the reclamated soil. On the one hand, the question about environmental pollution made by the mushroom compost is solved, and on the other hand, it can improve the ... Keywords: mushroom material, micro-organisms, soil quality

Liu Xueran; Li Xinju; Li Bing

2010-03-01T23:59:59.000Z

127

Live Theater Wetland Kayak Trip  

E-Print Network (OSTI)

Live Theater Wetland Kayak Trip Adapted from http://water.usgs.gov/outreach/poster2/grade footprints(squish squish) in the mud with every pass. The shore was lined with tall cattails, but we found doused in sun screen, with me in the front and Mrs. Findley doing all the work steering in the back

Texas at Austin, University of

128

Field Guide: Live Working Rope  

Science Conference Proceedings (OSTI)

Ropes are an essential tool in many applications associated with the electric power industry. Ropes used in proximity to or in contact with high-voltage power lines require demanding dielectric properties as well as strength and durability. A comprehensive understanding of live working rope use is necessary for safe and efficient operations wherever such ropes are used.

2009-03-31T23:59:59.000Z

129

Raman activity in synchronously dividing bacteria  

SciTech Connect

Using a spectrometer equipped with an optical-multichannel analyzer as the detector (OMA), we have observed the Stokes laser-Raman spectra of metabolically active Escherichia coli and Bacillus megaterium from 100 - 2100 cm/sup -1/. After lengthy investigation, no Raman lines attributable to the metabolic process nor the cells themselves were found. Previous Raman spectra of active bacteria cannot be used to support nonlinear theories in biology. 34 refs., 9 figs.

Layne, S.P.

1985-01-01T23:59:59.000Z

130

How sulphate-reducing microorganisms cope with stress: Lessons from systems biology  

E-Print Network (OSTI)

DH10B: insights into the biology of a laboratory workhorse.146. Kitano, H. Systems biology: a brief overview. ScienceAHandbookon the Biologyof Bacteria: Proteobacteria:

Zhou, J.

2011-01-01T23:59:59.000Z

131

Natural Oil Production from Microorganisms: Bioprocess and Microbe Engineering for Total Carbon Utilization in Biofuel Production  

Science Conference Proceedings (OSTI)

Electrofuels Project: MIT is using carbon dioxide (CO2) and hydrogen generated from electricity to produce natural oils that can be upgraded to hydrocarbon fuels. MIT has designed a 2-stage biofuel production system. In the first stage, hydrogen and CO2 are fed to a microorganism capable of converting these feedstocks to a 2-carbon compound called acetate. In the second stage, acetate is delivered to a different microorganism that can use the acetate to grow and produce oil. The oil can be removed from the reactor tank and chemically converted to various hydrocarbons. The electricity for the process could be supplied from novel means currently in development, or more proven methods such as the combustion of municipal waste, which would also generate the required CO2 and enhance the overall efficiency of MITs biofuel-production system.

None

2010-07-15T23:59:59.000Z

132

Biotechnology--Improving Our Lives Through  

E-Print Network (OSTI)

transgenic plants and microorganisms to produce inexpensive, renewable sources of fuel and energy.S. but are not currently on the market. #12;Herbicide-Tolerant Crops Allow Adoption of No-till Practices http-tolerant technology as the key factor in doing so. Conservation Tillage Information Center (2002) Purdue University

Hammock, Bruce D.

133

3rd ASM Conference on Cell-Cell Communication in Bacteria  

Science Conference Proceedings (OSTI)

This report summarizes the final program and provides the abstracts presented at the fourth American Society of Microbiology-sponsored conference on Cell-cell Communication in Bacteria, held November 6-9, 2011 in Miami, Florida. Bacteria are the paradigm for unicellular life, yet they also exhibit elaborate coordinated behaviors that often defy unicellularity. Research over the past two decades has revealed that a wide range of microbes communicate by diverse mechanisms. In most cases these microbial conversations occur through the exchange of diffusible signals, although there are also clear examples of contact-dependent communication. Many microbes use these signaling mechanisms to monitor and respond to population density, a process often described as quorum sensing. Interbacterial communication is not, however restricted to quorum sensing mechanisms, and there is mounting evidence that signaling can function in a range of different capacities. Communication between microorganisms has profound impacts on host interactions, as pathogens and commensals often regulate factors critical for interaction with their hosts via signal production and perception. The CCCB-4 conference provided a unique forum for the discussion, dissemination and exchange of new information and ideas among researchers working within this rapidly developing, yet mature field. Sessions were arranged around topics such as: the diversity of signal generation and identity; mechanisms of signal transduction and interference; cell-cell communication in bacterial development and antibiotic synthesis; host-microbe signaling and pathogenesis; symbiosis, mutualism, and microbe-microbe communication; ecology and evolution; advancements in the technological tool-kit for studying cell-cell communication. The conference served as a conduit for the exchange and synthesis of new ideas among leading US and international scientists working on bacterial communication.

Nalker, Lisa K. [ASM

2011-11-06T23:59:59.000Z

134

Detection of phenols using engineered bacteria  

DOE Patents (OSTI)

Detection of phenols using engineered bacteria. A biosensor can be created by placing a reporter gene under control of an inducible promoter. The reporter gene produces a signal when a cognate transcriptional activator senses the inducing chemical. Creation of bacterial biosensors is currently restricted by limited knowledge of the genetic systems of bacteria that catabolize xenobiotics. By using mutagenic PCR to change the chemical specificity of the Pseudomonas species CF600 DmpR protein, the potential for engineering novel biosensors for detection of phenols has been demonstrated. DmpR, a well-characterized transcriptional activator of the P. CF600's dmp operon mediates growth on simple phenols. Transcription from Po, the promoter heading the dmp operon, is activated when the sensor domain of DmpR interacts with phenol and mono-substituted phenols. By altering the sensor domain of the DmpR, a group of DmpR derivatives that activate transcription of a Po-lacZ fusion in response to eight of the EPA's eleven priority pollutant phenols has been created. The assays and the sensor domain mutations that alter the chemical specificity of DmpR is described.

Wise, Arlene A. (Philadelphia, PA); Kuske, Cheryl R. (Los Alamos, NM); Terwilliger, Thomas C. (Santa Fe, NM)

2007-12-04T23:59:59.000Z

135

Living Direct: Order (2011-CE-1904)  

Energy.gov (U.S. Department of Energy (DOE))

DOE ordered Living Direct, Inc. to pay a $6,000 civil penalty after finding Living Direct had failed to certify that certain models of dishwashers, refrigerator-freezers and freezers comply with the applicable energy conservation standards.

136

Advanced Biofuels: How Scientists are Engineering Bacteria to Help Drive  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Advanced Biofuels: How Scientists are Engineering Bacteria to Help Advanced Biofuels: How Scientists are Engineering Bacteria to Help Drive America Advanced Biofuels: How Scientists are Engineering Bacteria to Help Drive America December 6, 2011 - 2:12pm Addthis Strains of E. coli bacteria were engineered to digest switchgrass biomass and synthesize its sugars into gasoline, diesel and jet fuel. | Image courtesy of Berkeley Lab. Strains of E. coli bacteria were engineered to digest switchgrass biomass and synthesize its sugars into gasoline, diesel and jet fuel. | Image courtesy of Berkeley Lab. Liisa O'Neill Liisa O'Neill Former New Media Specialist, Office of Public Affairs Who knew Escherichia coli (E. coli) could help America reduce its dependence on foreign oil? A breakthrough with the bacteria could make it cheaper to produce fuel from switchgrass -- an advanced biofuel with the

137

Advanced Biofuels: How Scientists are Engineering Bacteria to Help Drive  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Advanced Biofuels: How Scientists are Engineering Bacteria to Help Advanced Biofuels: How Scientists are Engineering Bacteria to Help Drive America Advanced Biofuels: How Scientists are Engineering Bacteria to Help Drive America December 6, 2011 - 2:12pm Addthis Strains of E. coli bacteria were engineered to digest switchgrass biomass and synthesize its sugars into gasoline, diesel and jet fuel. | Image courtesy of Berkeley Lab. Strains of E. coli bacteria were engineered to digest switchgrass biomass and synthesize its sugars into gasoline, diesel and jet fuel. | Image courtesy of Berkeley Lab. Liisa O'Neill Liisa O'Neill Former New Media Specialist, Office of Public Affairs Who knew Escherichia coli (E. coli) could help America reduce its dependence on foreign oil? A breakthrough with the bacteria could make it cheaper to produce fuel from switchgrass -- an advanced biofuel with the

138

EPRI Live Working Reference Book, Third Edition  

Science Conference Proceedings (OSTI)

This report is a fully revised, updated, and expanded edition of the EPRI Live Working Reference Book, Second Edition (1024479), published by EPRI in 2011. The EPRI Live Working Reference Book, Third Edition provides the utility industry with a single, comprehensive, updated guidebook on live working standards, tools, and procedures.Results and ...

2012-12-31T23:59:59.000Z

139

EPRI Live Working Reference Book, Ed. 4  

Science Conference Proceedings (OSTI)

This report is a fully revised, updated, and expanded edition of the EPRI Live Working Reference Book, Third Edition (1024142), published by EPRI in 2012. The EPRI Live Working Reference Book, Fourth Edition (3002000891) provides the utility industry with a single, comprehensive, updated guidebook on live working standards, tools, and procedures.BackgroundDeregulation demands higher levels of ...

2013-12-27T23:59:59.000Z

140

Single Cell Oils: Microbial and Algal Oils, 2nd EditionChapter 11 Carotenoid Production Using Microorganisms  

Science Conference Proceedings (OSTI)

Single Cell Oils: Microbial and Algal Oils, 2nd Edition Chapter 11 Carotenoid Production Using Microorganisms Biofuels and Bioproducts and Biodiesel Biofuels - Bioproducts eChapters Press Downloadable pdf of Chapt

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


141

Live Fire Range Environmental Assessment  

SciTech Connect

The Central Training Academy (CTA) is a DOE Headquarters Organization located in Albuquerque, New Mexico, with the mission to effectively and efficiently educate and train personnel involved in the protection of vital national security interests of DOE. The CTA Live Fire Range (LFR), where most of the firearms and tactical training occurs, is a complex separate from the main campus. The purpose of the proposed action is to expand the LFR to allow more options of implementing required training. The Department of Energy has prepared this Environmental Assessment (EA) for the proposed construction and operation of an expanded Live Fire Range Facility at the Central Training Academy in Albuquerque, New Mexico. Based on the analysis in the EA, DOE has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an environmental impact statement is not required and DOE is issuing this Finding of No Significant Impact (FONSI).

None

1993-08-01T23:59:59.000Z

142

Genetically engineered acidophilic heterotrophic bacteria by bacteriophage transduction  

DOE Patents (OSTI)

A bacteriophage capable of infecting acidophilic heterotrophic bacteria and processes for genetically engineering acidophilic bacteria for biomining or sulfur removal from coal are disclosed. The bacteriophage is capable of growth in cells existing at pH at or below 3.0. Lytic forms of the phage introduced into areas experiencing acid drainage kill the bacteria causing such drainage. Lysogenic forms of the phage having genes for selective removal of metallic or nonmetallic elements can be introduced into acidophilic bacteria to effect removal of the desired element from ore or coal. 1 fig., 1 tab.

Ward, T.E.; Bruhn, D.F.; Bulmer, D.F.

1989-05-10T23:59:59.000Z

143

Antenna organization in green photosynthetic bacteria  

DOE Green Energy (OSTI)

This project is concerned with the structure and function of the unique antenna system found in the green photosynthetic bacteria. The antenna system in these organisms is contained within a vesicle known as a chlorosome, which is attached to the cytoplasmic side of the cell membrane. Additional antenna pigments and reaction centers are contained in integral membrane proteins. Energy absorbed by the bacteriochlorophyll c (BChl c) pigments in the chlorosome is transferred via a baseplate'' array of BChl a antenna pigments into the membrane and to the reaction center. A schematic model of chlorosome structure is shown. This project is aimed at increasing our understanding of the organization of the pigments in the chlorosome and how the antenna system functions.

Blankenship, R.E.

1987-01-01T23:59:59.000Z

144

Energy conversion in Purple Bacteria Photosynthesis  

E-Print Network (OSTI)

The study of how photosynthetic organisms convert light offers insight not only into nature's evolutionary process, but may also give clues as to how best to design and manipulate artificial photosynthetic systems -- and also how far we can drive natural photosynthetic systems beyond normal operating conditions, so that they can harvest energy for us under otherwise extreme conditions. In addition to its interest from a basic scientific perspective, therefore, the goal to develop a deep quantitative understanding of photosynthesis offers the potential payoff of enhancing our current arsenal of alternative energy sources for the future. In the following Chapter, we consider the trade-off between dynamics, structure and function of light harvesting membranes in Rps. Photometricum purple bacteria, as a model to highlight the priorities that arise when photosynthetic organisms adapt to deal with the ever-changing natural environment conditions.

Caycedo-Soler, Felipe; Quiroga, Luis; Zhao, Guannan; Johnson, Neil F

2011-01-01T23:59:59.000Z

145

Energy conversion in Purple Bacteria Photosynthesis  

E-Print Network (OSTI)

The study of how photosynthetic organisms convert light offers insight not only into nature's evolutionary process, but may also give clues as to how best to design and manipulate artificial photosynthetic systems -- and also how far we can drive natural photosynthetic systems beyond normal operating conditions, so that they can harvest energy for us under otherwise extreme conditions. In addition to its interest from a basic scientific perspective, therefore, the goal to develop a deep quantitative understanding of photosynthesis offers the potential payoff of enhancing our current arsenal of alternative energy sources for the future. In the following Chapter, we consider the trade-off between dynamics, structure and function of light harvesting membranes in Rps. Photometricum purple bacteria, as a model to highlight the priorities that arise when photosynthetic organisms adapt to deal with the ever-changing natural environment conditions.

Felipe Caycedo-Soler; Ferney J. Rodriguez; Luis Quiroga; Guannan Zhao; Neil F. Johnson

2011-07-01T23:59:59.000Z

146

Live Cinema: designing an instrument for cinema editing as a live performance  

Science Conference Proceedings (OSTI)

This paper describes the design of an expressive tangible interface for cinema editing as a live performance. A short survey of live video practices is provided. The Live Cinema instrument is a cross between a musical instrument and a film editing tool, ... Keywords: DJ, VJ, film editing, improvisation, live cinema, narrative structure, performance, tactile interface, two-hand interaction, video controller, visual music

Michael Lew

2004-06-01T23:59:59.000Z

147

BioNLP 2011 task bacteria biotope: the Alvis system  

Science Conference Proceedings (OSTI)

This paper describes the system of the INRA Bibliome research group applied to the Bacteria Biotope (BB) task of the BioNLP 2011 shared tasks. Bacteria, geographical locations and host entities were processed by a pattern-based approach and domain lexical ...

Zorana Ratkovic; Wiktoria Golik; Pierre Warnier; Philippe Veber; Claire Ndellec

2011-06-01T23:59:59.000Z

148

Argonne cleans contaminated Kansas site by feeding bacteria | Argonne  

NLE Websites -- All DOE Office Websites (Extended Search)

Argonne cleans contaminated Kansas site by feeding bacteria Argonne cleans contaminated Kansas site by feeding bacteria By Jared Sagoff * October 8, 2010 Tweet EmailPrint When cleaning the bathroom, we usually consider bacteria the enemy. However, a new study conducted by environmental scientists at the U.S. Department of Energy's Argonne National Laboratory has demonstrated a way to enlist bacteria in the fight to cleanse some of the country's most intractably polluted locations. Last year, a team of Argonne scientists led by Lorraine LaFreniere injected iron microparticles underneath fields long-polluted with carbon tetrachloride near Centralia, Kansas. The researchers coated the microparticles with organic material, which served as bait for bacteria that created the conditions necessary to safely convert the toxic chemical

149

Ropes for Live Working and Energized Rescue  

Science Conference Proceedings (OSTI)

EPRI's Ropes for Live Working report (1015912, 2008) was the culmination of several years of research on the subject of live working ropes. As part of that research, some 45 samples of 12 different ropes were tested electrically at the EPRI-Lenox laboratory. The objective of the 2011 project, summarized in this report, was to identify performance requirements of rope suitable for live work and energized rescue. Based on experience in other disciplines, particularly mountaineering, Kernmantle rope is sui...

2011-12-16T23:59:59.000Z

150

2012 Tillingate Living Case Study Feedback Report  

Science Conference Proceedings (OSTI)

... traumatic brain injury, but it does not provide ... strategic planning and objectives do not appear ... Living ensures its ability to execute the strategic plan, ...

2013-06-04T23:59:59.000Z

151

WEB: The Living Textbook of Nuclear Chemistry  

Science Conference Proceedings (OSTI)

Jul 2, 2008 ... The American Chemical Society's website "The Living Textbook of Nuclear Chemistry" provides 12 videos related to the history of nuclear...

152

Heterogeneous electron transfer studies with ligninolytic redox enzymes and living bacteria. Applications in biosensors and biofuel cells.  

E-Print Network (OSTI)

??The catalytic properties and the inter-domain electron transfer of cellobiose dehydrogenase (CDH) from the ascomycete fungus Myriococcum thermophilum adsorbed on graphite and thiol (SAM) modified (more)

Coman, Vasile

2009-01-01T23:59:59.000Z

153

Systematic characterization of protein glycosylation of bacteria cell surface proteins  

NLE Websites -- All DOE Office Websites (Extended Search)

Bacteria cell Bacteria cell Insoluble fraction Glycoprotein Enrichment Integrated top-down and bottom-up Glycoprotein & Glycopeptide Step 1: Glycoproteome profile Glycans HILIC-FTICR-MS/MS (Sequencing ) Step 2: Glycan profile NMR (structure recognization) Data Interpretation Databases De Novo and other algorithms Step 3: Glycoinformatics Glycan database Glycoprotein database Hydrolysis graphitized carbon cloumn Schematic Representation of Proposed Platform for Bacterial Glycoproteome Characterization EMSL Research and Capability Development Proposals Systematic characterization of protein glycosylation of bacteria cell surface proteins Project start date: July 2011 Principal Investigator: Si Wu Mass Spectrometry and Magnet Resonance Group, EMSL, PNNL Co-investigators:

154

Living technology: Exploiting life's principles in technology  

Science Conference Proceedings (OSTI)

The concept of living technology---that is, technology that is based on the powerful core features of life---is explained and illustrated with examples from artificial life software, reconfigurable and evolvable hardware, autonomously self-reproducing ... Keywords: Living technology, World Wide Web, autonomous robot, protocell, scientific social responsibility, synthetic biology

Mark A. Bedau; John S. McCaskill; Norman H. Packard; Steen Rasmussen

2010-01-01T23:59:59.000Z

155

Nano scale devices for plasmonic nanolithography and rapid sensing of bacteria  

E-Print Network (OSTI)

This dissertation contains two different research topics. One is a Nano Scale Device for Plasmonic Nanolithography Optical Antenna and the other is a Nano Scale Device for Rapid Sensing of Bacteria SEPTIC. Since these two different research topics have little analogy to each other, they were divided into different chapters throughout the whole dissertation. The Optical Antenna and Nanowell / Microwell / ISFET Sensor represent the device names of each topic Plasmonic Nanolithography and Rapid Sensing of Bacteria, respectively. For plasmonic nanolithography, we demonstrated a novel photonic device - Optical Antenna (OA) - that works as a nano scale object lens. It consists of a number of sub-wavelength features in a metal film coated on a quartz substrate. The device focuses the incident light to form a narrow beam in the near-field and even far-field region. The narrow beam lasts for up to several wavelengths before it diverges. We demonstrated that the OA was able to focus a subwavelength spot with a working distance (also the focal length) of several m, theoretically and experimentally. The highest imaging resolution (90-nm spots) is more than a 100% improvement of the diffraction limit (FWHM = 210 nm) in conventional optics. A model and 3D electromagnetic simulation results were also studied. Given its small footprint and subwavelength resolution, the PL holds great promise in direct-writing and scanning microscopy. Collaborative work demonstrated a Nanowell (or Microwell) device which enables a rapid and specific detection of bacteria using nano (or micro) scale probe to monitor the electric field fluctuations caused by ion leakage from the bacteria. When a bacteriophage infects a bacterium and injects its DNA into the host cell, a massive and transitory ion efflux from the host cell occurs. SEPTIC (SEnsing of Phage-Triggered Ion Cascade) technology developed by collaboration uses a nanowell device to detect the nano-scale electric field fluctuations caused by this ion efflux. The SEPTIC provides fast (within several minutes), effective (living cell only), phage specific (simple and less malfunction), cheap, compact and robust method for bacteria sensing. We fabricated a number of devices, including Nanowell, Microwell, and ISFET (Ion Selective Field Effect Transistor), which detect bacteria-phage reactions in frequency domain and time domain. In the frequency domain, detected noise spectrum is characterized by ? f / 1 . The positive reaction showed much higher 1 ? ? than that of background noise or negative reaction ( 0 ? ? ). For the time domain, we observed abnormal pulses (> ? 8 ) lasting 0.1 ~ 0.3 s which match the duration of ion flux reported by prior literatures. And the ISFET showed the phage-infection-triggered pulse in the form of the deviated drain current. Given the size of nanowell (or microwell, ISFET) and the simplified detection electronics, the cost of bacteria sensing is significantly reduced and the robustness is well improved, indicating very promising applications in clinical diagnosis and bio-defense.

Seo, Sungkyu

2007-12-01T23:59:59.000Z

156

Nano scale devices for plasmonic nanolithography and rapid sensing of bacteria  

E-Print Network (OSTI)

This dissertation contains two different research topics. One is a "Nano Scale Device for Plasmonic Nanolithography - Optical Antenna' and the other is a 'Nano Scale Device for Rapid Sensing of Bacteria - SEPTIC'. Since these two different research topics have little analogy to each other, they were divided into different chapters throughout the whole dissertation. The 'Optical Antenna' and 'Nanowell / Microwell / ISFET Sensor' represent the device names of each topic 'Plasmonic Nanolithography' and 'Rapid Sensing of Bacteria' respectively. For plasmonic nanolithography, we demonstrated a novel photonic device - Optical Antenna (OA) - that works as a nano scale object lens. It consists of a number of sub-wavelength features in a metal film coated on a quartz substrate. The device focuses the incident light to form a narrow beam in the near-field and even far-field region. The narrow beam lasts for up to several wavelengths before it diverges. We demonstrated that the OA was able to focus a subwavelength spot with a working distance (also the focal length) of several m, theoretically and experimentally. The highest imaging resolution (90-nm spots) is more than a 100% improvement of the diffraction limit (FWHM = 210 nm) in conventional optics. A model and 3D electromagnetic simulation results were also studied. Given its small footprint and subwavelength resolution, the PL holds great promise in direct-writing and scanning microscopy. Collaborative work demonstrated a Nanowell (or Microwell) device which enables a rapid and specific detection of bacteria using nano (or micro) scale probe to monitor the electric field fluctuations caused by ion leakage from the bacteria. When a bacteriophage infects a bacterium and injects its DNA into the host cell, a massive and transitory ion efflux from the host cell occurs. SEPTIC (SEnsing of Phage-Triggered Ion Cascade) technology developed by collaboration uses a nanowell device to detect the nano-scale electric field fluctuations caused by this ion efflux. The SEPTIC provides fast (within several minutes), effective (living cell only), phage specific (simple and less malfunction), cheap, compact and robust method for bacteria sensing. We fabricated a number of devices, including 'Nanowell', 'Microwell' and 'ISFET (Ion Selective Field Effect Transistor)', which detect bacteria-phage reactions in frequency domain and time domain. In the frequency domain, detected noise spectrum is characterized by 1/f[beta]. The positive reaction showed much higher [beta] =?1 than that of background noise or negative reaction ( [beta] =?0). For the time domain, we observed abnormal pulses (> 8[omega] ) lasting 0.1 ~ 0.3 s which match the duration of ion flux reported by prior literatures. And the ISFET showed the phage-infection-triggered pulse in the form of the deviated drain current. Given the size of nanowell (or microwell, ISFET) and the simplified detection electronics, the cost of bacteria sensing is significantly reduced and the robustness is well improved, indicating very promising applications in clinical diagnosis and bio-defense.

Seo, Sungkyu

2007-12-01T23:59:59.000Z

157

Evaluacin y seleccin de microorganismos para la produccin de etanol a nivel industrial = Evaluation and selection of microorganisms for ethanol production at industrial level.  

E-Print Network (OSTI)

??Mariscal Moreno, Juan Pablo (2011) Evaluacin y seleccin de microorganismos para la produccin de etanol a nivel industrial = Evaluation and selection of microorganisms for (more)

Mariscal Moreno, Juan Pablo

2011-01-01T23:59:59.000Z

158

Development and Evaluation of Methods to Infer Biosynthesis and Substrate Consumption in Cultures of Cellulolytic Microorganisms  

NLE Websites -- All DOE Office Websites (Extended Search)

and and Evaluation of Methods to Infer Biosynthesis and Substrate Consumption in Cultures of Cellulolytic Microorganisms Evert K. Holwerda, Lucas D. Ellis, Lee R. Lynd Thayer School of Engineering at Dartmouth College, 14 Engineering Drive, Hanover, New Hampshire, 03755; telephone: 1-6036462231; fax: 1-6036462277; e-mail: lee.r.lynd@dartmouth.edu ABSTRACT: Concentrations of biosynthate (microbial bio- mass plus extracellular proteins) and residual substrate were inferred using elemental analysis for batch cultures of Clostridium thermocellum. Inferring residual substrate based on elemental analysis for a cellulose (Avicel)-grown culture shows similar results to residual substrate determined by quantitative saccharification using acid hydrolysis. Inference based on elemental analysis is also compared to different on- line measurements: base addition, CO

159

Environmental assessment of the potential effects of aquifer thermal energy storage systems on microorganisms in groundwater  

DOE Green Energy (OSTI)

The primary objective of this study was to evaluate the potential environmental effects (both adverse and beneficials) of aquifer thermal energy storage (ATES) technology pertaining to microbial communities indigenous to subsurface environments (i.e., aquifers) and the propagation, movement, and potential release of pathogenic microorganisms (specifically, Legionella) within ATES systems. Seasonal storage of thermal energy in aquifers shows great promise to reduce peak demand; reduce electric utility load problems; contribute to establishing favorable economics for district heating and cooling systems; and reduce pollution from extraction, refining, and combustion of fossil fuels. However, concerns that the widespread implementation of this technology may have adverse effects on biological systems indigeneous to aquifers, as well as help to propagate and release pathogenic organisms that enter thee environments need to be resolved. 101 refs., 2 tabs.

Hicks, R.J.; Stewart, D.L.

1988-03-01T23:59:59.000Z

160

How sulphate-reducing microorganisms cope with stress: Lessons from systems biology  

Science Conference Proceedings (OSTI)

Sulphate-reducing microorganisms (SRMs) are a phylogenetically diverse group of anaerobes encompassing distinct physiologies with a broad ecological distribution. As SRMs have important roles in the biogeochemical cycling of carbon, nitrogen, sulphur and various metals, an understanding of how these organisms respond to environmental stresses is of fundamental and practical importance. In this Review, we highlight recent applications of systems biology tools in studying the stress responses of SRMs, particularly Desulfovibrio spp., at the cell, population, community and ecosystem levels. The syntrophic lifestyle of SRMs is also discussed, with a focus on system-level analyses of adaptive mechanisms. Such information is important for understanding the microbiology of the global sulphur cycle and for developing biotechnological applications of SRMs for environmental remediation, energy production, biocorrosion control, wastewater treatment and mineral recovery.

Zhou, J.; He, Q.; Hemme, C.L.; Mukhopadhyay, A.; Hillesland, K.; Zhou, A.; He, Z.; Nostrand, J.D. Van; Hazen, T.C.; Stahl, D.A.; Wall, J.D.; Arkin, A.P.

2011-04-01T23:59:59.000Z

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity  

DOE Patents (OSTI)

This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70.degree. C. to 90.degree. C., at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%.

Premuzic, Eugene T. (East Moriches, NY); Lin, Mow (Rocky Point, NY)

1996-02-20T23:59:59.000Z

162

Development of Equipment to Separate Nonthermal and Thermal Effects of Radio Frequency Energy on Microorganisms  

SciTech Connect

A radio frequency (RF) dielectric heater has been developed for isolating thermal and nonthermal effects of RF energy on microorganisms in liquid foods. The modified heater enables the simultaneous application of RF energy and removal of thermal energy from the liquids. A double-pipe heat exchanger is an integral part of the heater. The outer pipe is made of Teflon. The inner pipe is made of stainless steel that is grounded in the RF circuit. Liquid food flows through the annular region between the two concentric pipes. Cooling water flows through the stainless steel pipe. The food in the annular region absorbs the RF energy. Concurrently, the cooling water flowing in the inner pipe removes the thermal energy from the food, thus controlling the temperature.

D.J. Geveke; M. Kozempel; C. Brunkhorst

1999-11-01T23:59:59.000Z

163

Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels  

SciTech Connect

The ability to generate microorganisms that can produce biofuels similar to petroleum-based transportation fuels would allow the use of existing engines and infrastructure and would save an enormous amount of capital required for replacing the current infrastructure to accommodate biofuels that have properties significantly different from petroleum-based fuels. Several groups have demonstrated the feasibility of manipulating microbes to produce molecules similar to petroleum-derived products, albeit at relatively low productivity (e.g. maximum butanol production is around 20 g/L). For cost-effective production of biofuels, the fuel-producing hosts and pathways must be engineered and optimized. Advances in metabolic engineering and synthetic biology will provide new tools for metabolic engineers to better understand how to rewire the cell in order to create the desired phenotypes for the production of economically viable biofuels.

Kuk Lee, Sung; Chou, Howard; Ham, Timothy S.; Soon Lee, Taek; Keasling, Jay D.

2009-12-02T23:59:59.000Z

164

Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity  

DOE Patents (OSTI)

This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70 C to 90 C, at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%. 68 figs.

Premuzic, E.T.; Lin, M.

1996-02-20T23:59:59.000Z

165

Why sequence Alkaliphilic sulfur oxidizing bacteria for sulfur pollution  

NLE Websites -- All DOE Office Websites (Extended Search)

Alkaliphilic sulfur oxidizing Alkaliphilic sulfur oxidizing bacteria for sulfur pollution remediation? Burning sulfur-containing fuels, such as coal, oil, and natural gas, contributes significantly to global environmental problems, such as air pollution and acid rain, besides contributing to the loss of the ozone layer. One method of managing sulfur compounds released as byproducts from industrial processes is to scrub them out using chemical treatments and activated charcoal beds. A lower-cost solution relies on incorporating alkaliphic sulfur-oxidizing bacteria into biofilters to convert the volatile and toxic compounds into insoluble sulfur for easier removal. Discovered in the last decade, these bacteria have been found to thrive in habitats that span the full pH range. The bacteria could have applications

166

Micro-scale interactions between chemotactic bacteria and algae  

E-Print Network (OSTI)

Traditional views of marine environments describe the ocean pelagic zone as a homogeneous nutrient-poor environment. Heterotrophic marine bacteria that have evolved high-energy mechanisms for swimming abilities and sensing ...

Vahora, Nisha

2010-01-01T23:59:59.000Z

167

Isolation and identification of fuel-oil-degrading bacteria.  

E-Print Network (OSTI)

??The purpose of this study is to isolate and identify the crude oil-degrading bacteria from oil polluted soil. Their physiological characteristics and oil-degrading capability were (more)

Yang, Wan-yu

2008-01-01T23:59:59.000Z

168

A C. elegans-based foam for rapid on-site detection of residual live virus.  

Science Conference Proceedings (OSTI)

In the response to and recovery from a critical homeland security event involving deliberate or accidental release of biological agents, initial decontamination efforts are necessarily followed by tests for the presence of residual live virus or bacteria. Such 'clearance sampling' should be rapid and accurate, to inform decision makers as they take appropriate action to ensure the safety of the public and of operational personnel. However, the current protocol for clearance sampling is extremely time-intensive and costly, and requires significant amounts of laboratory space and capacity. Detection of residual live virus is particularly problematic and time-consuming, as it requires evaluation of replication potential within a eukaryotic host such as chicken embryos. The intention of this project was to develop a new method for clearance sampling, by leveraging Sandia's expertise in the biological and material sciences in order to create a C. elegans-based foam that could be applied directly to the entire contaminated area for quick and accurate detection of any and all residual live virus by means of a fluorescent signal. Such a novel technology for rapid, on-site detection of live virus would greatly interest the DHS, DoD, and EPA, and hold broad commercial potential, especially with regard to the transportation industry.

Negrete, Oscar A.; Branda, Catherine; Hardesty, Jasper O. E. (Sandia National Laboratories, Albuquerque, NM); Tucker, Mark David (Sandia National Laboratories, Albuquerque, NM); Kaiser, Julia N. (Global Product Management, Hilden, Germany); Kozina, Carol L.; Chirica, Gabriela S.

2012-02-01T23:59:59.000Z

169

Efficient Living Energy Grant | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Efficient Living Energy Grant Efficient Living Energy Grant Efficient Living Energy Grant < Back Eligibility Low-Income Residential Savings Category Heating & Cooling Commercial Heating & Cooling Heating Cooling Appliances & Electronics Heat Pumps Commercial Lighting Lighting Water Heating Other Maximum Rebate $350,000 or 100% of the total project costs Program Info State Illinois Program Type State Grant Program Rebate Amount Varies by technology Provider Smart Energy Design Assistance Center The Smart Energy Design Assistance Center and the Illinois Department of Commerce and Economic Opportunity is offering grants for public housing authorities PHAs) and their residents for the implementation of energy efficiency measures. Applicants must be serviced by Ameren Illinois, ComEd,

170

Bacterial and Archaea Community Present in the Pine Barrens Forest of Long Island, NY: Unusually High Percentage of Ammonia Oxidizing Bacteria  

Science Conference Proceedings (OSTI)

Of the few preserved areas in the northeast of United States, the soil in the Pine Barrens Forests presents a harsh environment for the microorganisms to grow and survive. In the current study we report the use of clustering methods to scientifically select the sampling locations that would represent the entire forest and also report the microbial diversity present in various horizons of the soil. Sixty six sampling locations were selected across the forest and soils were collected from three horizons (sampling depths). The three horizons were 0-10 cm (Horizon O); 11-25 cm (Horizon A) and 26-40 cm (Horizon B). Based on the total microbial substrate utilization pattern and K-means clustering analysis, the soil in the Pine Barrens Forest can be classified into four distinct clusters at each of the three horizons. One soil sample from each of the four clusters were selected and archaeal and bacterial populations within the soil studied using pyrosequencing method. The results show the microbial communities present in each of these clusters are different. Within the microbial communities present, microorganisms involved in nitrogen cycle occupy a major fraction of microbial community in the soil. High level of diversity was observed for nitrogen fixing bacteria. In contrast, Nitrosovibrio and Nitrosocaldus spp are the single bacterial and archaeal population respectively carrying out ammonia oxidation in the soil.

Shah, V.; Green, T.; Shah, V.; Shah, S.; Kambhampati, M.; Ambrose, J.; Smith, N.; Dowd, S.; McDonnell, K.; Panigrahi, B.

2011-10-20T23:59:59.000Z

171

Evaluation of the In Situ Aerobic Cometabolism of Chlorinated Ethenes by Toluene-Utilizing Microorganisms Using Push-Pull Tests  

DOE Green Energy (OSTI)

Single-well-push-pull tests were used in a contaminated aquifer to evaluate the ability of toluene-oxidizing microorganisms to aerobically cometabolize chlorinated aliphatic hydrocarbons (CAHs) such as trichloroethene (TCE). Groundwater containing dissolved toluene was injected into the saturated zone in biostimulate indigenous toluene-utilizers. The test solution was injected into the aquifer using a standard monitoring well and then was transported under natural-gradient conditions. Transport tests demonstrated similar transport characteristics of the conservative tracer and the reactive solutes. Biostimulation tests were then performed by injecting a test solution containing dissolved toluene substrate, hydrogen peroxide, bromide and nitrate in order to increase the biomass of toluene-utilizing microorganisms. During the biostimulation tests, decreases in toluene concentration and the production of o-cresol as an intermediate oxidation product, indicated the simulation of toluene-utilizing microorganisms containing an ortho-monooxygenase enzyme. Transformation tests conducted after biostimulation demonstrated that indigenous microorganisms have the capability to transform the surrogate compounds (e.g. isobutene). Isobutene was transformed to isobutene oxide, indicating transformation by a toluene ortho-monooxygenase.

Azizian, Mohammad F.; Istok, Jonathan; Semprini, Lewis

2004-03-31T23:59:59.000Z

172

Bioaugmentation of butane-utilizing microorganisms to promote cometabolism of 1,1,1-trichloroethane in groundwater microcosms  

E-Print Network (OSTI)

Bioaugmentation of butane-utilizing microorganisms to promote cometabolism of 1,1,1-trichloroethane. The initial inoculum for bioaugmentation was a butane-utilizing enrichment from the subsurface of the Hanford DOE site. The non-augmented microcosm required 80 days of incubation before butane

Semprini, Lewis

173

Bioprocessing of lignite coals using reductive microorganisms. Final technical report, September 30, 1988--March 29, 1992  

SciTech Connect

In order to convert lignite coals into liquid fuels, gases or chemical feedstock, the macromolecular structure of the coal must be broken down into low molecular weight fractions prior to further modification. Our research focused on this aspect of coal bioprocessing. We isolated, characterized and studied the lignite coal-depolymerizing organisms Streptomyces viridosporus T7A, Pseudomonas sp. DLC-62, unidentified bacterial strain DLC-BB2 and Gram-positive Bacillus megaterium strain DLC-21. In this research we showed that these bacteria are able to solubilize and depolymerize lignite coals using a combination of biological mechanisms including the excretion of coal solublizing basic chemical metabolites and extracellular coal depolymerizing enzymes.

Crawford, D.L.

1992-03-29T23:59:59.000Z

174

Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702  

DOE Patents (OSTI)

Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

Dees, H. Craig (Lenoir City, TN)

1998-01-01T23:59:59.000Z

175

Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702  

DOE Patents (OSTI)

Bacteria which produce large amounts of cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

Dees, H. Craig (Lenoir City, TN)

1997-12-16T23:59:59.000Z

176

Solar Living Institute | Open Energy Information  

Open Energy Info (EERE)

Living Institute Living Institute Jump to: navigation, search Name Solar Living Institute Address 13771 S. Hwy. 101 Place Hopland, California Zip 95449 Region Bay Area Notes Non-profit educational organization whose mission is to promote sustainable living through inspirational environmental education Website http://www.solarliving.org/ Coordinates 38.9845018°, -123.117395° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":38.9845018,"lon":-123.117395,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

177

Rural Living Canada Website | Open Energy Information  

Open Energy Info (EERE)

Rural Living Canada Website Rural Living Canada Website Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Rural Living Canada Website Focus Area: Renewable Energy Topics: Policy, Deployment, & Program Impact Website: rurallivingcanada.4t.com/Pag00167.htm Equivalent URI: cleanenergysolutions.org/content/rural-living-canada-website Language: English Policies: "Deployment Programs,Regulations" is not in the list of possible values (Deployment Programs, Financial Incentives, Regulations) for this property. DeploymentPrograms: Training & Education Regulations: Net Metering & Interconnection This website is a compendium of Canadian non-urban energy access resources and websites since 1998. The website lists several resources for rural communities that cover more than just energy related technologies or

178

Endosymbiosis In Statu Nascendi: Close Phylogenetic RelationshipBetween Obligately Endosymbiotic and Obligately Free-LivingPolynucleobacter Strains (Betaproteobacteria)  

Science Conference Proceedings (OSTI)

Bacterial strains affiliated to the phylogenetically shallowsubcluster C (PnecC) of the 28 Polynucleobacter cluster, which ischaracterized by a minimal 16S rRNA gene sequence similarity of approx.98.5 percent, have been reported to occur as obligate endosymbionts of 30ciliates (Euplotes spp.), as well as to occur as free-living cells in thepelagic zone of freshwater habitats. We investigated if these two groupsof closely related bacteria represent 32 strains fundamentally differingin lifestyle, or if they simply represent different stages of afacultative endosymbiotic lifestyle. The phylogenetic analysis of 16SrRNA gene and 16S34 23S ITS sequences of five endosymbiont strains fromtwo different Euplotes species and 40 pure culture strains demonstratedhost-species-specific clustering of the endosymbiont 36 sequences withinthe PnecC subcluster. The sequences of the endosymbionts showedcharacteristics indicating an obligate endosymbiotic lifestyle.Cultivation experiments 38 revealed fundamental differences inphysiological adaptations, and determination of the genome sizesindicated a slight size reduction in endosymbiotic strains. We concludethat the 40 two groups of PnecC bacteria represent obligately free-livingand obligately endosymbiotic strains, respectively, and do not representdifferent stages of the same complex lifecycle. 42 These closely relatedstrains occupy completely separated ecological niches. To our bestknowledge, this is the closest phylogenetic relationship between obligateendosymbionts and 44 obligately free-living bacteria everrevealed.

Vannini, Claudia; Pockl, Matthias; Petroni, Giulio; Wu, Qinglong; Lang, Elke; Stackebrandt, Erko; Schrallhammer, Martina; Richardson, PaulM.; Hahn, Martin W.

2006-07-21T23:59:59.000Z

179

Computational phenotype prediction of ionizing-radiation-resistant bacteria with a multiple-instance learning model  

Science Conference Proceedings (OSTI)

Ionizing-radiation-resistant bacteria (IRRB) are important in biotechnology. The use of these bacteria for the treatment of radioactive wastes is determined by their surprising capacity of adaptation to radionuclides and a variety of toxic molecules. ... Keywords: ionizing-radiation-resistant bacteria, ionizing-radiation-sensitive bacteria, multiple-instance learning, phenotypic prediction, protein sequences

Sabeur Aridhi; Mondher Maddouri; Haitham Sghaier; Engelbert Mephu Nguifo

2013-08-01T23:59:59.000Z

180

EPRI Live Working Reference Book Edition 2  

Science Conference Proceedings (OSTI)

This report contains updated information on the 2012 edition of the National Electrical Safety Code (NESC) and on glass insulators that became available after publication of the first edition of the Tan Book (Electric Power Research Institute [EPRI] report 1018974) in 2009. The 2009 first edition of the this report was a fully revised, updated, and expanded version of the Live Working Guide for Overhead Lines (EPRI report 1008747), published in 2004. The new EPRI Live Working Reference Book provides the ...

2011-11-02T23:59:59.000Z

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

WATCH LIVE: Talking the Higgs Boson with CERN | Department of...  

NLE Websites -- All DOE Office Websites (Extended Search)

WATCH LIVE: Talking the Higgs Boson with CERN WATCH LIVE: Talking the Higgs Boson with CERN September 13, 2012 - 2:33pm Addthis WATCH LIVE: Talking the Higgs Boson with CERN...

182

Portsmouth Site Feeds Bacteria to Render Hazardous Groundwater Waste  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Portsmouth Site Feeds Bacteria to Render Hazardous Groundwater Portsmouth Site Feeds Bacteria to Render Hazardous Groundwater Waste Harmless Portsmouth Site Feeds Bacteria to Render Hazardous Groundwater Waste Harmless April 2, 2012 - 12:00pm Addthis Neil Smith puts a trained eye on the pressure and flow of a food-grade com¬pound being injected into an under¬ground plume of hazardous waste near the X-720 Maintenance Facility at the DOE Piketon Site. The sodium lactate compound promotes bacterial growth in the groundwater that turns hazardous waste into harmless end-products. Neil Smith puts a trained eye on the pressure and flow of a food-grade com¬pound being injected into an under¬ground plume of hazardous waste near the X-720 Maintenance Facility at the DOE Piketon Site. The sodium lactate compound promotes bacterial growth in the groundwater that turns

183

The catabolism of phenanthrene and naphthalene by bacteria  

E-Print Network (OSTI)

Thirteen strains of bacteria able to grow on phenanthrene were isolated from soil; they included fluorescent and non-fluorescent pseudomonads, vibrios and unidentified bacteria. Two of the pseudomonads, like Aeromonas sp. ~45~1, also grew on naphthalene. In all strains, growth on phenanthrene induced the enzyme responsible for the conversion of 1-hydroxy-2-naphthoate to 2-carboxybenzaldehyde, NAD-dependent 2-carboxybenzaldehyde dehydrogenase and protocatechuate oxygenase, but not salicylate hydroxylase, catechol oxygenase or NAD(P)H-dependent 1-hydroxy-2-naphthoate hydroxylase. Growth on naphthalene induced salicylate hydroxylase and catechol oxygenase. It is suggested that the catabolism of phenanthrene occurs via protocatechuate in all these bacteria, and that the pathways for degradation of phenanthrene and naphthalene are separate.

H. Kiyohara; K. Nagao

1978-01-01T23:59:59.000Z

184

U-062: Pidgin SILC (Secure Internet Live Conferencing) Protocol...  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

2: Pidgin SILC (Secure Internet Live Conferencing) Protocol Denial of Service Vulnerability U-062: Pidgin SILC (Secure Internet Live Conferencing) Protocol Denial of Service...

185

Green Living, Green Technologies: Things to Be Thankful For ...  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Centers Field Sites Power Marketing Administration Other Agencies You are here Home Green Living, Green Technologies: Things to Be Thankful For Green Living, Green...

186

University Teams to Showcase Affordable, Energy Efficient Living...  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

to Showcase Affordable, Energy Efficient Living in U.S. Department of Energy Solar Decathlon 2011 University Teams to Showcase Affordable, Energy Efficient Living in U.S....

187

living walls | OpenEI Community  

Open Energy Info (EERE)

14 14 Varnish cache server Home Groups Community Central Green Button Applications Developer Utility Rate FRED: FRee Energy Database More Public Groups Private Groups Features Groups Blog posts Content Stream Documents Discussions Polls Q & A Events Notices My stuff Energy blogs 429 Throttled (bot load) Error 429 Throttled (bot load) Throttled (bot load) Guru Meditation: XID: 2142229614 Varnish cache server living walls Home Dc's picture Submitted by Dc(15) Member 15 November, 2013 - 13:26 Living Walls ancient building system architect biomimicry building technology cooling cu daylight design problem energy use engineer fred andreas geothermal green building heat transfer heating living walls metabolic adjustment net zero pre-electricity Renewable Energy Solar university of colorado utility grid Wind

188

LiveFuels Inc | Open Energy Information  

Open Energy Info (EERE)

LiveFuels Inc LiveFuels Inc Jump to: navigation, search Name LiveFuels Inc Address 1300 Industrial Road Place San Carlos, California Zip 94070 Sector Biofuels Product Produces a biocrude from open-pond algae bioreactors Website http://www.livefuels.com/ Coordinates 37.500518°, -122.246433° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":37.500518,"lon":-122.246433,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

189

Community is Live!! | OpenEI Community  

Open Energy Info (EERE)

Community is Live!! Community is Live!! Home > Groups > OpenEI Community Central Graham7781's picture Submitted by Graham7781(2002) Super contributor 25 June, 2012 - 16:10 imported OpenEI OpenEI Community section is now live! You can join the threads and talk to the people behind OpenEI, as well as other users of OpenEI. Our slick interface allows you to blog, question, comment, create events, and even create your own groups so you can invite members to discuss your topics. The platform was built by OpenEI developers using Acquia Commons, free social business software with a wide range of modules that fit inside of Drupal. OpenEI is excited to have this functionality, allowing our users to become more engaged, and help continue to drive OpenEI future to best represent its users.

190

Early Detection Saves Lives | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Early Detection Saves Lives Early Detection Saves Lives Early Detection Saves Lives September 20, 2012 - 2:39pm Addthis What does this mean for me? It can save your life. It can save the life of someone you love. The Worker Health Protection Program (WHPP) is the DOE's Former Worker Medical Screening Program at 13 DOE sites. WHPP provides free medical evaluations for selected occupational diseases every three years to eligible former DOE workers under a national medical protocol established by the DOE. WHPP is funded by the DOE and is operated by a consortium of the City University of New York, the United Steelworkers, and the Atomic Trades & Labor Council in association with clinical facilities in communities near DOE sites. The sites covered under WHPP include the K-25 Gaseous Diffusion Plant, Y-12 National Security Complex, Oak Ridge National

191

Living Walls | OpenEI Community  

Open Energy Info (EERE)

Living Walls Living Walls Home > Groups > Buildings Dc's picture Submitted by Dc(15) Member 15 November, 2013 - 13:26 ancient building system architect biomimicry building technology cooling cu daylight design problem energy use engineer fred andreas geothermal green building heat transfer heating living walls metabolic adjustment net zero pre-electricity Renewable Energy Solar university of colorado utility grid Wind Much of the discussion surrounding green buildings centers around reducing energy use. The term net zero is the platinum standard for green buildings, meaning the building in question does not take any more energy from the utility grid than it produces using renewable energy resources, such as solar, wind, or geothermal installations (and sometimes these renewable energy resources actually feed energy back to the utility grid). Architects

192

Live Working Tools for High Temperature Conductors  

Science Conference Proceedings (OSTI)

In long-duration (several days) tests, strain link sticks used for live work were removed from service and exposed to conductors operating at high temperature of about 250-260C. Only strain link sticks were tested to date. Results obtained do not indicate damage or deterioration of the tested sticks. The research is a joint effort between project 35.010 Live Working Research for Overhead Transmission Equipment, Techniques, Procedures and Protective Grounding and project 35.015 Advanced Conductors to inve...

2010-12-17T23:59:59.000Z

193

DEVELOPMENT OF MICROORGANISMS WITH IMPROVED TRANSPORT AND BIOSURFACTANT ACTIVITY FOR ENHANCED OIL RECOVERY  

Science Conference Proceedings (OSTI)

Diverse microorganisms were screened for biosurfactant production and anaerobic growth at elevated salt concentrations to obtain candidates most suitable for microbial oil recovery. Seventy percent of the 205 strains tested, mostly strains of Bacillus mojavensis, Bacillus subtilis, Bacillus licheniformis, and Bacillus sonorensis, produced biosurfactants aerobically and 41% of the strains had biosurfactant activity greater than Bacillus mojavensis JF-2, the current candidate for oil recovery. Biosurfactant activity varied with the percentage of the 3-hydroxy-tetradecanoate isomers in the fatty acid portion of the biosurfactant. Changing the medium composition by incorporation of different precursors of 3-hydroxy tetradecanoate increased the activity of biosurfactant. The surface tension and critical micelle concentration of 15 different, biosurfactant-producing Bacillus strains was determined individually and in combination with other biosurfactants. Some biosurfactant mixtures were found to have synergistic effect on surface tension (e.g. surface tension was lowered from 41 to 31 mN/m in some cases) while others had a synergistic effect on CMD-1 values. We compared the transport abilities of spores from three Bacillus strains using a model porous system to study spore recovery and transport. Sand-packed columns were used to select for spores or cells with the best transport abilities through brine-saturated sand. Spores of Bacillus mojavensis strains JF-2 and ROB-2 and a natural recombinant, strain C-9, transported through sand at very high efficiencies. The earliest cells/spores that emerged from the column were re-grown, allowed to sporulate, and applied to a second column. This procedure greatly enhanced the transport of strain C-9. Spores with enhanced transport abilities can be easily obtained and that the preparation of inocula for use in MEOR is feasible. Tertiary oil recovery experiments showed that 10 to 40 mg/l of JF-2 biosurfactant in the presence of 0.1 mM 2,3-butanediol and 1 g/l of partially hydrolyzed polyacrylamide (PHPA) recovered 10-40% of residual oil from Berea sandstone cores. When PHPA was used alone, about 10% of the residual oil was recovered. Interfacial tension (IFT) decreased in a stepwise manner as biosurfactant concentration increased with marked reductions in IFT occurring at biosurfactant concentrations of 10 and 40 mg/l. When the biosurfactant concentration was greater than 10 mg/l, residual oil recovery linearly increased with biosurfactant concentration. A mathematical model that relates oil recovery to biosurfactant concentration was modified to include the stepwise changes in IFT as biosurfactant concentrations changes. This model adequately predicted the experimentally observed changes in IFT as a function of biosurfactant concentration. Our work shows that (1) diverse microorganisms produce biosurfactants, (2) nutrient manipulation may provide a mechanism to increase biosurfactant activity, (3) biosurfactant concentrations in excess of the critical micelle concentration recover substantial amounts of residual oil, and (4) equations that describe the effect of the biosurfactant on IFT adequately predict residual oil recovery in sandstone cores.

M.J. McInerney; N. Youssef; T. Fincher; S.K. Maudgalya; M.J. Folmsbee; R. Knapp; D. Nagle

2004-05-31T23:59:59.000Z

194

Legionnaires' Disease Bacteria in Power Plant Cooling Systems: Phase 2  

Science Conference Proceedings (OSTI)

Water temperature and quality, along with other aquatic organisms, affect the existence of infectious Legionella in power plant cooling water. However, the interaction of these factors is so complex that scientists are far from being able to predict the growth and infectivity of these bacteria.

1985-04-26T23:59:59.000Z

195

Live documents with contextual, data-driven information components  

Science Conference Proceedings (OSTI)

We introduce the notion of a live document and we describe our concept of live documents with contextual, data driven information components. The dynamic and interactive features of live documents provide a consistent data source for multimedia presentations ... Keywords: Microsoft Office, live documents, repurposing, reverse engineering, scalable vector graphics, single sourcing, software engineering, systems documentation

Anke Weber; Holger M. Kienle; Hausi A. Mller

2002-10-01T23:59:59.000Z

196

Forenscope: a framework for live forensics  

Science Conference Proceedings (OSTI)

Current post-mortem cyber-forensic techniques may cause significant disruption to the evidence gathering process by breaking active network connections and unmounting encrypted disks. Although newer live forensic analysis tools can preserve active state, ... Keywords: forensics, introspection, memory remanence

Ellick Chan; Shivaram Venkataraman; Francis David; Amey Chaugule; Roy Campbell

2010-12-01T23:59:59.000Z

197

The Live Room: transducing resonant architectures  

Science Conference Proceedings (OSTI)

The Live Room was a temporary site-specific installation presented in building N51, room 117 on the MIT campus on 7 May 1998 and concluded on 10 June 1998. Using small acoustic-intensifying equipment which mounted directly to the structure of ...

Mark Bain

2003-08-01T23:59:59.000Z

198

Adapting Your Home for More Accessible Living  

E-Print Network (OSTI)

It is important for people to live comfortably and independently in their homes. Homes can be adapted to aid people with various disabilities. This publication explains how to make such adaptations for people with vision loss, hearing loss, problems with touch and hand dexterity, loss of strength and range of motion, cognitive difficulties, mobility impairments, and problems with balance and coordination.

Harris, Janie

2007-04-27T23:59:59.000Z

199

Live Work with High Temperature Conductors  

Science Conference Proceedings (OSTI)

This report examines issues that may arise when live work is undertaken on conductors that operate at high temperatures (HT conductors) and provides the results from selected tests on the temperature levels reached by tools in contact with hot conductors. It also discusses possible concerns that may arise during de-energized work on lines that use HT conductors.

2009-12-15T23:59:59.000Z

200

Live Video Scheduling in Differentiated Services Internet  

Science Conference Proceedings (OSTI)

Future Internet services will be judged to a large degree by the efficient deployment of real-time applications. The issue of utilisation is especially central for the economic introduction of live video in packet switching networks; at the same time, ...

Themistoklis Rapsomanikis

2000-09-01T23:59:59.000Z

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

Live-structure dataflow analysis for Prolog  

Science Conference Proceedings (OSTI)

For the class of applicative programming languages, efficient methods for reclaiming the memory occupied by released data structures constitute an important aspect of current implementations. The present article addresses the problem of memory reuse ... Keywords: Prolog, abstract interpretation, compile-time garbage collection, liveness, program analysis

Anne Mulkers; William Winsborough; Maurice Bruynooghe

1994-03-01T23:59:59.000Z

202

Living Labs of Electric Vehicle Integration  

NLE Websites -- All DOE Office Websites (Extended Search)

Living Labs of Electric Vehicle Integration Living Labs of Electric Vehicle Integration Speaker(s): Johan Driesen Date: May 11, 2012 - 12:00pm Location: 90-3122 Seminar Host/Point of Contact: Chris Marnay Electric vehicles and plug-in hybrid vehicles are key to making transportation sustainable and climate change neutral. This talk will focus on the electricity grid integration aspects of wide-scale charging infrastructure: the impact on generation capacity, transmission and distribution are dealt with through measurements, modeling and scenario simulations. The advantages and problems of the possible business models to pay for the charging are discussed. Alternative charging and grid-coupling technology (e.g. wireless inductive charging) is considered. The relationship with the transition towards "smart cities" is discussed. In

203

SAFETY - A HABIT TO LIVE BY  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

SAFETY - A HABIT TO LIVE BY SAFETY - A HABIT TO LIVE BY WAPA F 1400.59# (7-91) United States Government Department of Energy memorandum Western Area Power Administration August 15, 2011 A7400 Supplement Analysis Environmental Review for the Montana-Alberta Tie Ltd. 230-kV Transmission Line Project (DOE/EIS-0399-SA-1) T. Meeks, A0000 Administrator Western Area Power Administration P. Hoffman Assistant Secretary, Department of Energy Office of Electricity Delivery and Energy Reliability Proposed Action: Modification to relocate four segments of the Montana-Alberta Tie Ltd. (MATL), 230-kV Transmission Line Project Corridor Proposed by: Montana-Alberta Tie Ltd. and MATL LLP (collectively, MATL) Background: In 2005, MATL proposed to construct and operate a 230-kV transmission line

204

Development of Video Camera for Live Working  

Science Conference Proceedings (OSTI)

This report is an update on the progress in the development of a small lightweight video camera for use in live work. The camera is designed to be attached to the end of an insulating tool, can be placed directly near or at the object to be observed, and can transmit the video (and audio) signal remotely to a receiver. The receiver can be located at the other end of the insulating tool (hotstick) or at any place convenient for the ...

2012-12-21T23:59:59.000Z

205

Fission barriers and half-lives  

Science Conference Proceedings (OSTI)

We briefly review the development of theoretical models for the calculation of fission barriers and half-lives. We focus on how results of actual calculations in a unified macroscopic-microscopic approach provide an interpretation of the mechanisms behind some of the large number of phenomena observed in fission. As instructive examples we choose studies of the rapidly varying fission properties of elements at the end of the periodic system. 31 refs., 10 figs.

Moeller, P.; Nix, J.R.; Swiatecki, W.J.

1989-01-01T23:59:59.000Z

206

Live Work on High Temperature Conductors  

Science Conference Proceedings (OSTI)

Feedback from field personnel working with high-temperature conductors indicates that when a dead-end compression yoke assembly (DCYA) is installed on the conductor according to normal utility procedures, the soft aluminum strands are deformed and "birdcage." This is of course a concern to the field crews and the utility operating the line. This report presents results of research and tests performed on selected conductors operating at high temperature (approximately 250-260C) with selected live wor...

2011-12-13T23:59:59.000Z

207

JGI - A Genomic Encyclopedia of Bacteria and Archaea  

NLE Websites -- All DOE Office Websites (Extended Search)

A Genomic Encyclopedia A Genomic Encyclopedia of Bacteria and Archaea (GEBA) The GEBA project is aimed at systematically filling in the gaps in sequencing along the bacterial and archaeal branches of the tree of life. Though the wide variety of microbial sequencing projects undertaken throughout the world has created a rich, diverse collection of microbial genomes, strong biases in what has been sequenced thus far are evident. This project represents the first systematic attempt to use the tree of life itself as a guide to sequencing target selection. JGI is beginning by collaborating on a pilot project with DSMZ. Why GEBA? The GEBA Pilot Project GEBA Sequencing Plans Interpret a Genome for Education Home > User Programs > A Genomic Encyclopedia of Bacteria and Archaea (GEBA)

208

Engineering aspects of hydrogen production from photosynthetic bacteria  

DOE Green Energy (OSTI)

Certain photosynthetic bacteria (PSB), for example, Rhodopseudomonas capsulata, evolve hydrogen when placed in an anaerobic environment with light and a suitable organic substrate. An engineering effort to use such bacteria for large-scale hydrogen production from sunlight is described. A system to produce 28,000 m/sup 3//day (1 x 10/sup 6/ ft/sup 3//day) of hydrogen has been designed on a conceptual level and includes hydrogen cleanup, substrate storage, and waste disposal. The most critical component in the design is the solar bacterial reactor. Several designs were developed and analyzed. A large covered pond concept appears most attractive. Cost estimates for the designs show favorable economics.

Herlevich, A.; Karpuk, M.

1982-02-01T23:59:59.000Z

209

Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio  

SciTech Connect

Sulfate reducing bacteria are physiologically important given their nearly ubiquitous presence and have important applications in the areas of bioremediation and bioenergy. This chapter provides details on the steps used for homologous-recombination mediated chromosomal manipulation of Desulfovibrio vulgaris Hildenborough, a well-studied sulfate reducer. More specifically, we focus on the implementation of a 'parts' based approach for suicide vector assembly, important aspects of anaerobic culturing, choices for antibiotic selection, electroporation-based DNA transformation, as well as tools for screening and verifying genetically modified constructs. These methods, which in principle may be extended to other sulfate-reducing bacteria, are applicable for functional genomics investigations, as well as metabolic engineering manipulations.

Chhabra, Swapnil R; Keller, Kimberly L.; Wall, Judy D.

2011-03-15T23:59:59.000Z

210

Oxidation and methylation of dissolved elemental mercury by anaerobic bacteria  

Science Conference Proceedings (OSTI)

Methylmercury is a neurotoxin that poses significant health risks to humans. Some anaerobic sulphate- and iron-reducing bacteria can methylate oxidized forms of mercury, generating methylmercury1-4. One strain of sulphate-reducing bacteria (Desulfovibrio desulfuricans ND132) can also methylate elemental mercury5. The prevalence of this trait among different bacterial strains and species remains unclear, however. Here, we compare the ability of two strains of the sulphate-reducing bacterium Desulfovibrio and one strain of the iron-reducing bacterium Geobacter to oxidise and methylate elemental mercury in a series of laboratory incubations. Experiments were carried out under dark, anaerobic conditions, in the presence of environmentally-relevant concentrations of elemental mercury. We report differences in the ability of these organisms to oxidise and methylate elemental mercury. In line with recent findings5, we show that Desulfovibrio desulfuricans ND132 can both oxidise and methylate elemental mercury. However, the rate of methylation of elemental mercury is only about one third the rate of methylation of oxidized mercury. We also show that Desulfovibrio alaskensis G20 can oxidise, but not methylate, elemental mercury. Geobacter sulfurreducens PCA is able to oxidise and methylate elemental mercury in the presence of cysteine. We suggest that the activity of methylating and non-methylating bacteria may together enhance the formation of methylmercury in anaerobic environments.

Hu, Haiyan [ORNL] [ORNL; Lin, Hui [ORNL] [ORNL; Zheng, Wang [ORNL] [ORNL; Tomanicek, Stephen J [ORNL] [ORNL; Johs, Alexander [ORNL] [ORNL; Feng, Xinbin [ORNL] [ORNL; Elias, Dwayne A [ORNL] [ORNL; Liang, Liyuan [ORNL] [ORNL; Liang, Liyuan [ORNL] [ORNL; Gu, Baohua [ORNL] [ORNL

2013-01-01T23:59:59.000Z

211

Development of Microorganisms with Improved Transport and Biosurfactant Activity for Enhanced Oil Recovery  

Science Conference Proceedings (OSTI)

The project had three objectives: (1) to develop microbial strains with improved biosurfactant properties that use cost-effective nutrients, (2) to obtain biosurfactant strains with improved transport properties through sandstones, and (3) to determine the empirical relationship between surfactant concentration and interfacial tension and whether in situ reactions kinetics and biosurfactant concentration meets appropriate engineering design criteria. Here, we show that a lipopeptide biosurfactant produced by Bacillus mojavensis strain JF-2 mobilized substantial amounts of residual hydrocarbon from sand-packed columns and Berea sandstone cores when a viscosifying agent and a low molecular weight alcohol were present. The amount of residual hydrocarbon mobilized depended on the biosurfactant concentration. Tertiary oil recovery experiments showed that 10 to 40 mg/l of JF-2 biosurfactant in the presence of 0.1 mM 2,3-butanediol and 1 g/l of partially hydrolyzed polyacrylamide (PHPA) recovered 10-40% of residual oil from Berea sandstone cores. Even low biosurfactant concentrations (16 mg/l) mobilized substantial amounts of residual hydrocarbon (29%). The bio-surfactant lowered IFT by nearly 2 orders of magnitude compared to typical IFT values of 28-29 mN/m. Increasing the salinity increased the IFT with or without 2,3-butanediol present. The lowest interfacial tension observed was 0.1 mN/m. A mathematical model that relates oil recovery to biosurfactant concentration was modified to include the stepwise changes in IFT as biosurfactant concentrations changes. This model adequately predicted the experimentally observed changes in IFT as a function of biosurfactant concentration. Theses data show that lipopeptide biosurfactant systems may be effective in removing hydrocarbon contamination sources in soils and aquifers and for the recovery of entrapped oil from low production oil reservoirs. Diverse microorganisms were screened for biosurfactant production and anaerobic growth at elevated salt concentrations to obtain candidates most suitable for microbial oil recovery. Seventy percent of the 205 strains tested, mostly strains of Bacillus mojavensis, Bacillus subtilis, Bacillus licheniformis, and Bacillus sonorensis, produced biosurfactants aerobically and 41% of the strains had biosurfactant activity greater than Bacillus mojavensis JF-2, the current candidate for oil recovery. Biosurfactant activity varied with the percentage of the 3-hydroxy-tetradecanoate isomers in the fatty acid portion of the biosurfactant. Changing the medium composition by incorporation of different precursors of 3-hydroxy tetradecanoate increased the activity of biosurfactant. The surface tension and critical micelle concentration of 15 different, biosurfactant-producing Bacillus strains was determined individually and in combination with other biosurfactants. Some biosurfactant mixtures were found to have synergistic effect on surface tension (e.g. surface tension was lowered from 41 to 31 mN/m in some cases) while others had a synergistic effect on CMD-1 values. We compared the transport abilities of spores from three Bacillus strains using a model porous system to study spore recovery and transport. Sand-packed columns were used to select for spores or cells with the best transport abilities through brine-saturated sand. Spores of Bacillus mojavensis strains JF-2 and ROB-2 and a natural recombinant, strain C-9, transported through sand at very high efficiencies. The earliest cells/spores that emerged from the column were regrown, allowed to sporulate, and applied to a second column. This procedure greatly enhanced the transport of strain C-9. Spores with enhanced transport abilities can be easily obtained and that the preparation of inocula for use in MEOR is feasible. We conducted a push-pull test to study in-situ biosurfactant production by exogenous biosurfactant producers to aid in oil recovery from depleted reservoirs. Five wells from the same formation were used. Two wells received cells and nutrients, two wells were treated with nutrients onl

M.J. McInerney; K.E. Duncan; N. Youssef; T. Fincher; S.K. Maudgalya; M.J. Folmsbee; R. Knapp; Randy R. Simpson; N.Ravi; D. Nagle

2005-08-15T23:59:59.000Z

212

Emergent patterns of diversity and dynamics in natural populations of planktonic Vibrio bacteria  

E-Print Network (OSTI)

Despite the importance of microorganisms for global and engineering processes, currently lacking is a theoretical framework to describe how the structure of a microbial assemblage translates an environmental condition into ...

Thompson, Janelle Rene, 1976-

2005-01-01T23:59:59.000Z

213

Live business intelligence for the real-time enterprise  

Science Conference Proceedings (OSTI)

We present our vision of a unified data management and analytics platform, which we call "Live Business Intelligence" (LiveBI) that transforms business intelligence from the traditional back-office, report-oriented platform, to an enabler for delivering ...

Malu Castellanos; Umeshwar Dayal; Meichun Hsu

2010-01-01T23:59:59.000Z

214

Intelligent Transportation Systems: Saving Lives, Time and Money Kristin Tufte  

E-Print Network (OSTI)

Intelligent Transportation Systems: Saving Lives, Time and Money Kristin Tufte Portland State University Oregon Transportation Summit Sept 10, 2010 #12;Intelligent Transportation Systems: Saving Lives, Time and Money Official transportation data archive for the Portland-Vancouver metropolitan region

Bertini, Robert L.

215

Acting Biomass Program Manager Dr. Valerie Reed to Host Live...  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Acting Biomass Program Manager Dr. Valerie Reed to Host Live Twitter Q&A on Advanced Biofuels Acting Biomass Program Manager Dr. Valerie Reed to Host Live Twitter Q&A on Advanced...

216

LIVE from the White House Science Fair | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

LIVE from the White House Science Fair LIVE from the White House Science Fair October 18, 2010 - 11:31am Addthis John Schueler John Schueler Former New Media Specialist, Office of...

217

Solar Decathlon Team Leading the Way Toward Sustainable Living...  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Team Leading the Way Toward Sustainable Living, Even in the Wake of Disasters Solar Decathlon Team Leading the Way Toward Sustainable Living, Even in the Wake of Disasters August...

218

Living the Vision: A Profile of Kathy Yelick  

NLE Websites -- All DOE Office Websites (Extended Search)

Living the Vision: A Profile of Kathy Yelick Living the Vision: A Profile of Kathy Yelick April 8, 2008 The February March 2008 issue of the European magazine Scientific...

219

Overview of Incidents Related to Live Working  

Science Conference Proceedings (OSTI)

This report is a summary of progress in the research on injury and fatality information among workers who perform energized (live) and/or de-energized work. While every effort is made in the industry to avoid incidents during work on energized and de-energized lines, they do occur, and there are lessons embedded within every incident from which the utility industry can benefit, if the incidents are thoroughly analyzed, root causes are identified, and corrective actions are taken. The primary objective of...

2006-03-30T23:59:59.000Z

220

Bursts of Active Transport in Living Cells  

E-Print Network (OSTI)

We scrutinize the temporally-resolved speed of active cargo transport in living cells, and show intermittent bursting motions. These nonlinear fluctuations follow a scaling law over several decades of time and space, the statistical regularities displaying a time-averaged shape that we interpret to reflect stress buildup followed by rapid release. The power law of scaling is the same as seen in driven jammed colloids, granular, and magnetic systems. The implied regulation of active transport with environmental obstruction extends the classical notion of molecular crowding.

Bo Wang; James Kuo; Steve Granick

2013-07-07T23:59:59.000Z

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

Investigation of Jacketed Rope for Live Work  

Science Conference Proceedings (OSTI)

Since the early 1990s, insulating tools have been used for live work (LW). Initially tools were made of wood and had homemade attachments, but fiberglass reinforced plastic (FRP) tools were introduced in late 1950s and replaced essentially all wooden tools. In view of the long time span since the previous major innovation in LW tools, the EPRI advisory task force of the LW project requested that research be performed on new tools, materials, and ropes that could serve as substitutes for some of the ...

2013-12-14T23:59:59.000Z

222

The DFKI competence center for ambient assisted living  

Science Conference Proceedings (OSTI)

The DFKI Competence Center for Ambient Assisted Living (CCAAL) is a cross-project and cross-department virtual organization within the German Research Center for Artificial Intelligence coordinating and conducting research and development in the area ... Keywords: ambient assisted living, intelligent environments, living labs

Jochen Frey; Christoph Stahl; Thomas Rfer; Bernd Krieg-Brckner; Jan Alexandersson

2010-11-01T23:59:59.000Z

223

Engineering Bacteria for Efficient Fuel Production: Novel Biological Conversion of Hydrogen and Carbon Dioxide Directly into Free Fatty Acids  

SciTech Connect

Electrofuels Project: OPX Biotechnologies is engineering a microorganism currently used in industrial biotechnology to directly produce a liquid fuel from hydrogen and carbon dioxide (CO2). The microorganism has the natural ability to use hydrogen and CO2 for growth. OPX Biotechnologies is modifying the microorganism to divert energy and carbon away from growth and towards the production of liquid fuels in larger, commercially viable quantities. The microbial system will produce a fuel precursor that can be chemically upgraded to various hydrocarbon fuels.

2010-07-12T23:59:59.000Z

224

JV between KP Renewables and Living Buildings | Open Energy Information  

Open Energy Info (EERE)

and Living Buildings and Living Buildings Jump to: navigation, search Name JV between KP Renewables and Living Buildings Place Brentford, Middlesex, Greater London, United Kingdom Zip TW8 9JJ Sector Biomass Product JV established to develop up to 15.0MW per year of small biomass based power projects on industrial sites in the UK. References JV between KP Renewables and Living Buildings[1] LinkedIn Connections CrunchBase Profile No CrunchBase profile. Create one now! This article is a stub. You can help OpenEI by expanding it. JV between KP Renewables and Living Buildings is a company located in Brentford, Middlesex, Greater London, United Kingdom . References ↑ "JV between KP Renewables and Living Buildings" Retrieved from "http://en.openei.org/w/index.php?title=JV_between_KP_Renewables_and_Living_Buildings&oldid=34782

225

Issues involved with non-characterized control of methanotrophic bacteria  

SciTech Connect

Methane-utilizing bacteria, methanotrophs, have application as biocatalysts in the commodity chemical production, waste treatment and environmental remediation industries. Methanotrophs have the ability to oxidize many chemical compounds into more desired products, such as the production of propylene oxide. Methanotrophs can also degrade toxic compounds such as trichloroethylene. However, there are many physical, chemical and biological problems associated with the continuous oxidation of chemicals. These include, low mass transfer of methane, oxygen and propylene; toxicity of substrates and degradation products, and competition between the growth substrate, i.e., methane and chemical feed stock, e.g., propylene for the biocatalyst. To supervise methanotrophic bioprocesses, an intelligent control system must accommodate any biological limitations, e.g., toxicity, and mitigate the impact of the physical and chemical limitations, e.g., mass transfer of methane and the solubility of propylene. The intelligent control system must have the capability to assess the current conditions and metabolic state of the bacteria; recognize and diagnose instrument faults; and select and maintain sets of parameters that will result in high production and growth.

Stoner, D.L.; Tolle, C.R.; Noah, K.S.; Davis, D.A.; Miller, K.S.; Fife, D.J.

1998-05-11T23:59:59.000Z

226

Lubricating bacteria model for branching growth of bacterial colonies, Phys  

E-Print Network (OSTI)

Various bacterial strains (e.g. strains belonging to the genera Bacillus, Paenibacillus, Serratia and Salmonella) exhibit colonial branching patterns during growth on poor semi-solid substrates. These patterns reflect the bacterial cooperative self-organization. Central part of the cooperation is the collective formation of lubricant on top of the agar which enables the bacteria to swim. Hence it provides the colony means to advance towards the food. One method of modeling the colonial development is via coupled reaction-diffusion equations which describe the time evolution of the bacterial density and the concentrations of the relevant chemical fields. This idea has been pursued by a number of groups. Here we present an additional model which specifically includes an evolution equation for the lubricant excreted by the bacteria. We show that when the diffusion of the fluid is governed by nonlinear diffusion coefficient branching patterns evolves. We study the effect of the rates of emission and decomposition of the lubricant fluid on the observed patterns. The results are compared with experimental observations. We also include fields of chemotactic agents and food chemotaxis and conclude that these features are needed in order to explain the observations. 1 I.

Yonathan Kozlovsky; Inon Cohen; Ido Golding; Eshel Ben-jacob

1999-01-01T23:59:59.000Z

227

A biological/chemical process for reduced waste and energy consumption, Caprolactam production: Phase 1, Select microorganisms and demonstrate feasibility. Final report  

DOE Green Energy (OSTI)

A novel biological/chemical process for converting cyclohexane into caprolactam was investigated. Microorganisms in a bioreactor would be used to convert cyclohexane into caprolactone followed by chemical synthesis of caprolactam using ammonia. The proposed bioprocess would be more energy efficient and reduce byproducts and wastes that are generated by the current chemical process. We have been successful in isolating from natural soil and water samples two microorganisms that can utilize cyclohexane as a sole source of carbon and energy for growth. These microorganisms were shown to have the correct metabolic intermediates and enzymes to convert cyclohexane into cyclohexanol, cyclohexanone and caprolactone. Genetic techniques to create and select for caprolactone hydrolase negative-mutants are being developed. These blocked-mutants will be used to convert cyclohexane into caprolactone but, because of the block, be unable to metabolize the caprolactone further and excrete it as a final end product.

St.Martin, E.J.

1995-08-01T23:59:59.000Z

228

Living on Long Island | Brookhaven National Laboratory  

NLE Websites -- All DOE Office Websites (Extended Search)

Careers at Brookhaven Careers at Brookhaven Home For Job Seekers Job List Life at Brookhaven Benefits Family Programs Recreation & Fitness Why Brookhaven? For New Hires For Employees Living on Long Island Stretching 118 miles from end to end and measuring no more than 20 miles at its widest point, Long Island was aptly named by Dutch traders who circum-navigated it in the early 1600s. Those early Dutchmen discovered what the native Indians had known for centuries: The temperate climate, the bountiful seas and the fertile land made Long Island a most hospitable home. Local Area Information Long Island Schools Parks Beaches Wineries New York City Today, Brookhaven National Laboratory sits in the geographical center of Long Island. To the west, New York City boasts Broadway shows, museums,

229

Liveness of Heap Data for Functional Programs  

E-Print Network (OSTI)

Functional programming languages use garbage collection for heap memory management. Ideally, garbage collectors should reclaim all objects that are dead at the time of garbage collection. An object is dead at an execution instant if it is not used in future. Garbage collectors collect only those dead objects that are not reachable from any program variable. This is because they are not able to distinguish between reachable objects that are dead and reachable objects that are live. In this paper, we describe a static analysis to discover reachable dead objects in programs written in first-order, eager functional programming languages. The results of this technique can be used to make reachable dead objects unreachable, thereby allowing garbage collectors to reclaim more dead objects.

Karkare, Amey; Sanyal, Amitabha

2007-01-01T23:59:59.000Z

230

Total half-lives for selected nuclides  

SciTech Connect

Measurements of the half-lives of {sup 3}H, {sup 10}Be, {sup 14}C, {sup 26}Al, {sup 40}K, {sup 39}Ar, {sup 53}Mn, {sup 87}Rb, {sup 92}Nb, {sup 129}I, {sup 138}La, {sup 147}Sm, {sup 176}Lu, {sup 174}Hf, {sup 180}Ta, {sup 187}Re, {sup 186}Os, {sup 190}Pt, {sup 204}Pb, {sup 210}Pb, {sup 210}Po, {sup 222}Rn, {sup 224}Th, {sup 226}Ra, {sup 227}Ac, {sup 228}Ra, {sup 228}Th, {sup 230}Th, {sup 232}Th, {sup 231}Pa have been compiled and evaluated. The effect of the {sup 14}C half-life value on carbon dating ages is discussed as well as the stability of {sup 204}Pb. 237 refs., 30 tabs.

Holden, N.E.

1989-01-01T23:59:59.000Z

231

Process for generation of hydrogen gas from various feedstocks using thermophilic bacteria  

SciTech Connect

A method for producing hydrogen gas is provided comprising selecting a bacteria from the Order Thermotogales, subjecting the bacteria to a feedstock and to a suitable growth environment having an oxygen concentration below the oxygen concentration of water in equilibrium with air; and maintaining the environment at a predetermined pH and at a temperature of at least approximately 45.degree. C. for a time sufficient to allow the bacteria to metabolize the feedstock.

Ooteghem, Suellen Van (Morgantown, WV)

2005-09-13T23:59:59.000Z

232

Process for Generation of Hydrogen Gas from Various Feedstocks Using Thermophilic Bacteria  

DOE Green Energy (OSTI)

A method for producing hydrogen gas is provided comprising selecting a bacteria from the Order Thermotogales, subjecting the bacteria to a feedstock and to a suitable growth environment having an oxygen concentration below the oxygen concentration of water in equilibrium with air; and maintaining the environment at a predetermined pH and at a temperature of at least approximately 45 degrees C. for a time sufficient to allow the bacteria to metabolize the feedstock.

Ooteghem Van, Suellen

2005-09-13T23:59:59.000Z

233

DOE Joint Genome Institute: Biofuel-Producing Bacteria, Insect Gut  

NLE Websites -- All DOE Office Websites (Extended Search)

June 29, 2009 June 29, 2009 Biofuel-Producing Bacteria, Insect Gut Microbes, ~ 70 other Projects Fill DOE Joint Genome Institute 2010 Pipeline WALNUT CREEK, CA-The U.S. Department of Energy (DOE) Joint Genome Institute (JGI) has selected 71 new genomic sequencing projects for its 2010 Community Sequencing Program (CSP)-a targeted sampling of the planet's biodiversity-to be characterized for bioenergy, climate, and environmental applications. JGI's Community Sequencing Program is the largest genomic sequencing effort in the world focused on nonmedical organisms, enabling scientists from universities and national laboratories to probe the hidden world of microbes and plants to tap nature's ingenuity for innovative solutions to the nation's major challenges in energy, climate, and environment. The

234

Plasma Enhanced Chemical Vapor Deposition on Living Substrates: Development, Characterization, and Biological Applications  

E-Print Network (OSTI)

This dissertation proposed the idea of plasma-enhanced chemical vapor deposition on living substrates (PECVD on living substrates) to bridge the gap between the thin film deposition technology and the biological and living substrates. This study focuses on the establishment of the knowledge and techniques necessary to perform PECVD on living substrates and contains three main aspects: development, characterization, and biological applications. First, a PECVD tool which can operate in ambient air and at low temperature was developed using a helium dielectric barrier discharge jet (DBD jet). It was demonstrated that various materials, such as polymeric, metallic, and composite films, can be readily synthesized through this technique. Second, the PMMA and copper films deposited using DBD jets were characterized. High-rate (22 nm/s), low-temperature (39 C) PMMA deposition was achieved and the film surface morphology can be tailored by altering the discharge power. Conductive copper films with an electrical resistivity lower than 110-7 ohm-m were obtained through hydrogen reduction. Both PMMA and copper films can be grown on temperature-sensitive substrates, such as plastics, pork skin, and even fingernail. The electrical, optical, and imaging characterization of the DBD jets was also conducted and several new findings were reported. Multiple short-duration current pulses instead of only one broad pulse per half voltage cycle were observed when a dielectric substrate was employed. Each short-duration current pulse is induced by a leading ionization wave followed by the formation of a plasma channel. Precursor addition further changed the temporal sequence of the pulses. An increase in the power led to a mode change from a diffuse DBD jet to a concentrated one. This mode change showed significant dependence on the precursor type, tube size, and electrode configuration. These findings regarding the discharge characteristics can thus facilitate the development of DBD-jet operation strategies to improve the deposition efficacy. Finally, this technique was used to grow PMMA films onto agar to demonstrate one of its potential biological applications: sterile bandage deposition. The DBD jet with the film depositing ability enabled the surface to be not only efficiently sanitized but also protected by a coating from being reached by bacteria.

Tsai, Tsung-Chan 1982-

2012-12-01T23:59:59.000Z

235

Brownsville Public Utilities Board - Green Living Residential Rebate  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Brownsville Public Utilities Board - Green Living Residential Brownsville Public Utilities Board - Green Living Residential Rebate Program Brownsville Public Utilities Board - Green Living Residential Rebate Program < Back Eligibility Residential Savings Category Home Weatherization Commercial Weatherization Heating & Cooling Commercial Heating & Cooling Cooling Sealing Your Home Ventilation Heat Pumps Appliances & Electronics Water Heating Windows, Doors, & Skylights Program Info State Texas Program Type Utility Rebate Program Provider Brownsville Public Utilities Board Brownsville Public Utilities Board offers residential customers rebates for installation of energy efficient measures. Through the Green Living Rebate program, customers can apply for rebates for installation of energy efficient HVAC, improved duct flow performance, Energy Star Windows, Energy

236

Consumer.Data.Gov is Live! | Data.gov  

NLE Websites -- All DOE Office Websites (Extended Search)

Consumer.Data.Gov is Live Consumer Data Apps Challenges Resources About Blogs Let's Talk Feedback Consumer You are here Data.gov Communities Consumer Blogs...

237

High Voltage Direct Current Live Line and Insulator Testing  

Science Conference Proceedings (OSTI)

This report has two main parts. The focus of the first part is on live work in overhead high-voltage direct current (HVDC) lines operating above 100 kV dc. It does not address issues related to lines for electric transport that typically operate below 60 kV dc worldwide. In addition, this first part of the report is not a detailed treatise on live work but, rather, addresses the main issues related to dc live work. More detailed information on the general topic of live work can be found in the references...

2011-12-23T23:59:59.000Z

238

Olson's Major World Ecosystem Complexes Ranked by Carbon in Live...  

NLE Websites -- All DOE Office Websites (Extended Search)

ecosystem complexes ranked by carbon in live vegetation: A Database. NDP-017, Carbon Dioxide Information Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee. This NDP was...

239

Tracking Living Cells as They Differentiate in Real Time  

NLE Websites -- All DOE Office Websites (Extended Search)

at the ALS, developed a new technique for monitoring protein phosphorylation inside living mammalian cells, enabling them to follow cellular chemical changes in real time,...

240

Bioaugmentation with butane-utilizing microorganisms to promote in situ cometabolic treatment of 1,1,1-trichloroethane and 1,1-dichloroethene  

E-Print Network (OSTI)

Bioaugmentation with butane-utilizing microorganisms to promote in situ cometabolic treatment of 1) through bioaugmentation with a butane enrichment culture containing predominantly two Rhodococcus sp of butane and dissolved oxygen and or hydrogen peroxide as sources of dissolved oxygen, about 70% removal

Semprini, Lewis

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


241

Detergent composition comprising a cellulase containing cell-free fermentate produced from microorganism ATCC 55702 or mutant thereof  

DOE Patents (OSTI)

Bacteria which produce large amounts of a cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

Dees, H. Craig (Lenoir City, TN)

1998-01-01T23:59:59.000Z

242

Clay enhancement of methane, low molecular weight hydrocarbon and halocarbon conversion by methanotrophic bacteria  

DOE Patents (OSTI)

An apparatus and method for increasing the rate of oxidation of toxic vapors by methanotrophic bacteria. The toxic vapors of interest are methane and trichloroethylene. The apparatus includes a gas phase bioreactor within a closed loop pumping system or a single pass system. The methanotrophic bacteria include Methylomonas methanica, Methylosinus trichosporium, and uncharacterized environmental enrichments.

Apel, William A. (Idaho Falls, ID); Dugan, Patrick R. (Idaho Falls, ID)

1995-01-01T23:59:59.000Z

243

PRODUCTION OF VOLATILE FATTY ACIDS BY STRICTLY ANAEROBIC BACTERIA IN THE DIGESTIVE TRACT OF GNOTOXENIC MICE.  

E-Print Network (OSTI)

SUMMARY PRODUCTION OF VOLATILE FATTY ACIDS BY STRICTLY ANAEROBIC BACTERIA IN THE DIGESTIVE TRACT OF « GNOTOXENIC » MICE. INHIBITORY EFFECT ON SHIGELLA FLEXNERI Various strains of strictly anaerobic bacteria of holoxenic animals, were implanted in the digestive tract of axenic mice. The in vivo production of VFA

Recanati, Catherine

244

8 OCTOBER 2009 TO PEER INSIDE A LIVING CELL  

E-Print Network (OSTI)

, capable of distinguishing even individual atoms. However, these microscopes cannot be used to image living molecules inside a living cell without disturbing them. Yanik and Putnam report their new approach bomb exploded about 30 m away. When exposed to such energetic electron beams, biological specimens

Yanik, Mehmet Fatih

245

A living lab research approach for mobile TV  

Science Conference Proceedings (OSTI)

More and more, a user centered approach is adopted for information and communication technologies (ICT) innovation research. One of the recently emerging concepts within this research tradition is the so called 'living lab'. A notable example of ICT ... Keywords: ict innovation, living lab, mobile tv, open innovation, teps, user centered research

Dimitri Schuurman; Tom Evens; Lieven De Marez

2009-06-01T23:59:59.000Z

246

A Living Lab research approach for mobile TV  

Science Conference Proceedings (OSTI)

More and more, a user-centered approach is adopted for information and communication technologies (ICT) innovation research. One of the recently emerging concepts within this research tradition is the so-called 'Living Lab'. Within this paper, we argue ... Keywords: ICT-adoption, Living Lab, Mobile TV, Open innovation, User-centric research

Dimitri Schuurman; Katrien De Moor; Lieven De Marez; Tom Evens

2011-11-01T23:59:59.000Z

247

Content pollution on P2P Live Streaming systems  

Science Conference Proceedings (OSTI)

P2P Live Streaming are subject to content pollution attack. This technology has high potential for the continuous consumption, however, the intentional degradation can become fatal for adherence and maintenance of users. This work deals with the characterization ... Keywords: Caracterizao, P2P Live Streaming, poluio

Joo Oliveira; Alex Borges; Srgio Campos

2009-10-01T23:59:59.000Z

248

Transportation Agency Tool to Analyze Benefits of Living Snow Fences  

E-Print Network (OSTI)

Transportation Agency Tool to Analyze Benefits of Living Snow Fences 5/31/12 Transportation Agency/31/12 Transportation Agency Tool to Analyze Benefits of Living Snow Fences Center for Integrated Natural Resources, Mobility, & Transportation Authority Benefits, Farmer Costs, & Carbon Impacts Focus Groups and Surveys

Minnesota, University of

249

Amateur vision and recreational orientation:: creating live video together  

Science Conference Proceedings (OSTI)

We explore the use of a live video broadcast system by a group of amateur camera operators to film an event on networked cameraphones. Using an interaction analysis of physical interactions and orientations to the work of others, we examine their choice ... Keywords: amateur, broadcast, collaboration, embodied interaction, leisure, live video, professional, user-generated content

Arvid Engstrm; Mark Perry; Oskar Juhlin

2012-02-01T23:59:59.000Z

250

Carbon nanotubes as photoacoustic molecular imaging agents in living mice  

E-Print Network (OSTI)

Carbon nanotubes as photoacoustic molecular imaging agents in living mice ADAM DE LA ZERDA1 not shown to target a diseased site in living subjects. Here we show that single-walled carbon nanotubes were verified ex vivo using Raman microscopy. Photoacoustic imaging of targeted single-walled carbon

Khuri-Yakub, Butrus T. "Pierre"

251

Investigate Use of and Requirements for Live Working Rope  

Science Conference Proceedings (OSTI)

This project includes studies to evaluate the performance of commercially available live working rope and prototype rope, analysis of how rope is used in the field and how it endures field use, and development of recommendations for storage, handling, testing, use and retirement of live working (LW) rope.

2006-12-01T23:59:59.000Z

252

National Nuclear Science Week live talks today | National Nuclear Security  

NLE Websites -- All DOE Office Websites (Extended Search)

Science Week live talks today | National Nuclear Security Science Week live talks today | National Nuclear Security Administration Our Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Continuing Management Reform Countering Nuclear Terrorism About Us Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Media Room Congressional Testimony Fact Sheets Newsletters Press Releases Speeches Events Social Media Video Gallery Photo Gallery NNSA Archive Federal Employment Apply for Our Jobs Our Jobs Working at NNSA Blog Home > NNSA Blog > National Nuclear Science Week live talks today National Nuclear Science Week live talks today Posted By Office of Public Affairs National Nuclear Science Week Students and teachers today will get the chance to talk live with nuclear

253

Interested in Open Energy Data? Watch the Energy Datapalooza Live! |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Interested in Open Energy Data? Watch the Energy Datapalooza Live! Interested in Open Energy Data? Watch the Energy Datapalooza Live! Interested in Open Energy Data? Watch the Energy Datapalooza Live! September 27, 2012 - 5:25pm Addthis U.S. Chief Technology Officer Todd Park kicks off the Safety Datapalooza on September 19th. | Photo Courtesy U.S. Department of Labor U.S. Chief Technology Officer Todd Park kicks off the Safety Datapalooza on September 19th. | Photo Courtesy U.S. Department of Labor Matthew Loveless Matthew Loveless Data Integration Specialist, Office of Public Affairs How can I participate? Watch the Datapalooza live at WhiteHouse.gov/live. On Monday, October 1st, more than 150 entrepreneurs, software developers, energy experts and policy makers are coming together for the first annual Energy Datapalooza. We'll be highlighting innovators that are using

254

Interested in Open Energy Data? Watch the Energy Datapalooza Live! |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Interested in Open Energy Data? Watch the Energy Datapalooza Live! Interested in Open Energy Data? Watch the Energy Datapalooza Live! Interested in Open Energy Data? Watch the Energy Datapalooza Live! September 27, 2012 - 5:25pm Addthis U.S. Chief Technology Officer Todd Park kicks off the Safety Datapalooza on September 19th. | Photo Courtesy U.S. Department of Labor U.S. Chief Technology Officer Todd Park kicks off the Safety Datapalooza on September 19th. | Photo Courtesy U.S. Department of Labor Matthew Loveless Matthew Loveless Data Integration Specialist, Office of Public Affairs How can I participate? Watch the Datapalooza live at WhiteHouse.gov/live. On Monday, October 1st, more than 150 entrepreneurs, software developers, energy experts and policy makers are coming together for the first annual Energy Datapalooza. We'll be highlighting innovators that are using

255

National Nuclear Science Week live talks today | National Nuclear Security  

National Nuclear Security Administration (NNSA)

Science Week live talks today | National Nuclear Security Science Week live talks today | National Nuclear Security Administration Our Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Continuing Management Reform Countering Nuclear Terrorism About Us Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Media Room Congressional Testimony Fact Sheets Newsletters Press Releases Speeches Events Social Media Video Gallery Photo Gallery NNSA Archive Federal Employment Apply for Our Jobs Our Jobs Working at NNSA Blog Home > NNSA Blog > National Nuclear Science Week live talks today National Nuclear Science Week live talks today Posted By Office of Public Affairs National Nuclear Science Week Students and teachers today will get the chance to talk live with nuclear

256

Plant Growth-Promoting Bacteria for Phytostabilization of Mine Tailings  

Science Conference Proceedings (OSTI)

Eolian dispersion of mine tailings in arid and semiarid environments is an emerging global issue for which economical remediation alternatives are needed. Phytostabilization, the revegetation of these sites with native plants, is one such alternative. Revegetation often requires the addition of bulky amendments such as compost which greatly increases cost. We report the use of plant growth-promoting bacteria (PGPB) to enhance the revegetation of mine tailings and minimize the need for compost amendment. Twenty promising PGPB isolates were used as seed inoculants in a series of greenhouse studies to examine revegetation of an extremely acidic, high metal content tailings sample previously shown to require 15% compost amendment for normal plant growth. Several isolates significantly enhanced growth of two native species, quailbush and buffalo grass, in tailings. In this study, PGPB/compost outcomes were plant specific; for quailbush, PGPB were most effective in combination with 10% compost addition while for buffalo grass, PGPB enhanced growth in the complete absence of compost. Results indicate that selected PGPB can improve plant establishment and reduce the need for compost amendment. Further, PGPB activities necessary for aiding plant growth in mine tailings likely include tolerance to acidic pH and metals.

Grandlic, C.J.; Mendez, M.O.; Chorover, J.; Machado, B.; Maier, R.M.

2009-05-19T23:59:59.000Z

257

Bioaugmentation with engineered endophytic bacteria improves contaminant fate in phytoremediation  

SciTech Connect

Phytoremediation of volatile organic contaminants often proves not ideal because plants and their rhizosphere microbes only partially degrade these compounds. Consequently, plants undergo evapotranspiration that contaminates the ambient air and, thus, undermines the merits of phytoremediation. Under laboratory conditions, endophytic bacteria equipped with the appropriate degradation pathways can improve in plant degradation of volatile organic contaminants. However, several obstacles must be overcome before engineered endophytes will be successful in field-scale phytoremediation projects. Here we report the first in situ inoculation of poplar trees, growing on a TCE-contaminated site, with the TCE-degrading strain Pseudomonas putida W619-TCE. In situ bioaugmentation with strain W619-TCE reduced TCE evapotranspiration by 90% under field conditions. This encouraging result was achieved after the establishment and enrichment of P. putida W619-TCE as a poplar root endophyte and by further horizontal gene transfer of TCE metabolic activity to members of the poplar's endogenous endophytic population. Since P. putida W619-TCE was engineered via horizontal gene transfer, its deliberate release is not restricted under European genetically modified organisms (GMO) regulations.

Weyens, N.; van der Lelie, D.; Artois, T.; Smeets, K.; Taghavi, S.; Newman, L.; Carleer, R.; Vangronsveld, J.

2009-12-01T23:59:59.000Z

258

Hydrogen (H2) Production by Anoxygenic Purple Nonsulfur Bacteria  

NLE Websites -- All DOE Office Websites (Extended Search)

produc8on by anoxygenic purple nonsulfur bacteria James 'Jake' McKinlay Assistant Professor, Biology Indiana University, Bloomington 4 N 2 + + 2NH 3 Purple n on---sulfur b acteria produce H 2 via n itrogenase biosynthe8c precursors and CO 2 central organic 'waste' metabolism compounds H + e --- Nitrogenase H 2 ATP Light (cyclic) energy photophosphoryla/on N 2 + 8H + + 8e - + 16ATP à H 2 + 2NH 4 + 8H + + 8e - + 16ATP à 4H 2 This is mode of photosynthesis does not produce oxygen Current state of the technology * H 2 yields - Growing : 10 - 25% of theoreIcal maximum - Non---growing: 40 - 91% of theoreIcal maximum * H 2 producIon rates L ---1 h --- - 10 - 82.5 ml H 2 L ---1 h ---1 over - 67 ml H 2 4000 h * Immobilized in 70 μm---thick latex film. Gosse et al. 2010. Biotechnol. P rog. 26: 907 - 18 * PhotosyntheIc efficiency: 1 - 2% - 6% Barbosa et al. 2001. J. Biotechnol. 8 5: 25---33 Reviewed

259

Estimating Bacteria Emissions from Inversion of Atmospheric Transport: Sensitivity to Modelled Particle Characteristics  

SciTech Connect

Model-simulated transport of atmospheric trace components can be combined with observed concentrations to obtain estimates of ground-based sources using various inversion techniques. These approaches have been applied in the past primarily to obtain source estimates for long-lived trace gases such as CO2. We consider the application of similar techniques to source estimation for atmospheric aerosols, by using as a case study the estimation of bacteria emissions from different ecosystem regions in the global atmospheric chemistry and climate model ECHAM5/MESSy-Atmospheric Chemistry (EMAC). Simulated particle concentrations in the tropopause region and at high latitudes, as well as transport of particles to tundra and land ice regions are shown to be highly sensitive to scavenging in mixed-phase clouds, which is poorly characterized in most global climate models. This may be a critical uncertainty in correctly simulating the transport of aerosol particles to the Arctic. Source estimation via Monte Carlo Markov Chain is applied to a suite of sensitivity simulations and the global mean emissions are estimated. We present an analysis of the partitioning of uncertainties in the global mean emissions that are attributable to particle size, CCN activity, the ice nucleation scavenging ratios for mixed-phase and cold clouds, and measurement error. Uncertainty due to CCN activity or to a 1 um error in particle size is typically between 10% and 40% of the uncertainty due to data uncertainty, as measured by the 5%-ile to 95%-ile range of the Monte Carlo ensemble. Uncertainty attributable to the ice nucleation scavenging ratio in mized-phase clouds is as high as 10% to 20% of the data uncertainty. Taken together, the four model 20 parameters examined contribute about half as much to the uncertainty in the estimated emissions as do the measurements. This was a surprisingly large contribution from model uncertainty in light of the substantial data uncertainty, which ranges from 81% to 870% for each of ten ecosystems for this case study. The effects of these and other model parameters in contributing to the uncertainties in the transport of atmospheric aerosol particles should be treated explicitly and systematically in both forward and inverse modelling studies.

Burrows, Susannah M.; Rayner, Perter; Butler, T.; Lawrence, M.

2013-06-04T23:59:59.000Z

260

Detecting and Tracking Nonfluorescent Nanoparticles Probes in Live Cells  

SciTech Connect

Precisely imaging and tracking dynamic biological processes in live cells are crucial for both fundamental research in life sciences and biomedical applications. Nonfluorescent nanoparticles are emerging as important optical probes in live-cell imaging because of their excellent photostability, large optical cross sections, and low cytotoxicity. Here, we provide a review of recent development in optical imaging of nonfluorescent nanoparticle probes and their applications in dynamic tracking and biosensing in live cells. A brief discussion on cytotoxicity of nanoparticle probes is also provided.

Wang, Gufeng; Fang, Ning

2012-01-17T23:59:59.000Z

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

Optimizing the Transmission Line Design for Effective Live Working  

Science Conference Proceedings (OSTI)

This report is intended to help designers of transmission lines to factor in the techniques and tools for live working when designing a new line. This draft progress report is being provided to members of EPRI's base-funded Live Working Research project for review and comment. A final version of the report will be included as part of a chapter in the revised Red Book, which is being rewritten in 2003 and 2004. Live work is the performance of maintenance, construction, or testing on equipment and circuits...

2003-08-28T23:59:59.000Z

262

Analysis of long-lived radionuclidic impurities in short-lived radiopharmaceutical waste using gamma spectrometry  

Science Conference Proceedings (OSTI)

Large hospitals and biomedical research centers utilize decay-in-storage programs to minimize the volume of their low level radioactive waste. However, some medically useful radionuclides often contain small amounts of long-lived radionuclidic impurities which may complicate simple waste management procedures. We have evaluated the extent of this problem in low level radioactive waste involving {sup 67}Cu and {sup 111}In over a 6-mo cycle of decay-in-storage by identifying the residual radionuclides in our dry waste using a multichannel analyzer. The multichannel analyzer was also used to quantify the radionuclide constituents of our liquid waste at the beginning of a decay-in-storage cycle. Radionuclides were identified by the presence of characteristic photopeaks of each isotope in the gamma spectrum and quantified by region of interest analysis. Gamma spectrometry can be used to aid waste segregation and final management decisions on low level radioactive waste. 10 refs., 1 fig., 2 tabs.

Salako, Q.; DeNardo, S.J. [Univ. of California, Sacramento, CA (United States)

1997-01-01T23:59:59.000Z

263

NREL: Continuum Magazine - A Living Laboratory for Energy Systems  

NLE Websites -- All DOE Office Websites (Extended Search)

Living Laboratory for Energy Systems Integration Living Laboratory for Energy Systems Integration Issue 4 Print Version Share this resource A Living Laboratory for Energy Systems Integration NREL is collecting, storing, analyzing, and displaying its building energy performance data to manage and optimize campus energy use. A screenshot of an energy dashboard which highlights the energy use for the South Table Mountain Campus at the National Renewable Energy Laboratory.' Enlarge image NREL's energy dashboard provides users with a bird's-eye view of energy use on the NREL campus. Illustration by Marjorie Schott, NREL The National Renewable Energy Laboratory (NREL) often prides itself on being a "living laboratory" for clean energy technologies. This became most apparent in 2011 with the opening of the Research Support Facility (RSF),

264

Stent Technology Saves Lives, Creates Jobs | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Stent Technology Saves Lives, Creates Jobs Stent Technology Saves Lives, Creates Jobs Stent Technology Saves Lives, Creates Jobs May 8, 2012 - 1:00pm Addthis The platinum/chromium alloy used in new coronary stents manufactured by Boston Scientific Corporation was developed by a research team that included metallurgists from NETL. The platinum/chromium alloy used in new coronary stents manufactured by Boston Scientific Corporation was developed by a research team that included metallurgists from NETL. Washington, DC - When people think of benefits from energy research, they usually don't envision saving lives. But thanks to an innovative alloy jointly developed by Boston Scientific Corporation (BSCI) and U.S. Department of Energy (DOE) metallurgists, that's what is happening. Using a special platinum/chromium alloy, BSCI has developed a line of

265

Tuesday Talk: Secretary Chu Answers Your Questions LIVE | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Tuesday Talk: Secretary Chu Answers Your Questions LIVE Tuesday Talk: Secretary Chu Answers Your Questions LIVE Tuesday Talk: Secretary Chu Answers Your Questions LIVE November 30, 2010 - 12:02pm Addthis John Schueler John Schueler Former New Media Specialist, Office of Public Affairs Later today Secretary Chu will be answering your questions as a part of the White House's Tuesday Talk series. The discussion will be broadcast live starting at 1:15 EST and will build off of the Secretary's speech at the National Press Club yesterday, which called on the United States to sharply accelerate innovations in clean energy. You can submit your questions for consideration via the White House's official Facebook page or Whitehouse.gov. You can also utilize the White House Facebook application to discuss the Tuesday Talk as it happens and

266

A Theory for Long-Lived Mesoscale Convective Systems  

Science Conference Proceedings (OSTI)

It is proposed that certain long-lived mesoscale convective systems maintain themselves through an interaction between quasi-balanced vertical motions and the diabatic effects of moist convection. Latent heat release, evaporation and melting of ...

D. J. Raymond; H. Jiang

1990-12-01T23:59:59.000Z

267

Watch a Rare Earth Elements Event Live This Morning | Department...  

NLE Websites -- All DOE Office Websites (Extended Search)

contractor to the Office of Public Affairs. Addthis Related Articles Watch Live: National Science Bowl - Starting At 9:30 AM ET President Barack Obama delivers his State of the...

268

A Theory for Strong, Long-Lived Squall Lines  

Science Conference Proceedings (OSTI)

We study herein the mechanics of long-lived, line-oriented, precipitating cumulus convection (squall lines) using two- and three-dimensional numerical models of moist convection. These models, used in juxtaposition, enable us to address the ...

Richard Rotunno; Joseph B. Klemp; Morris L. Weisman

1988-02-01T23:59:59.000Z

269

Living museum of the bay : Chesapeake Bay Aquarium  

E-Print Network (OSTI)

This thesis is the design of an aquarium in the industrial city of Newport News, Virginia The focus is a living museum that illustrates the diversity beauty and grandeur of a precious yet fragile estuary, the Chesapeake ...

Dickerson, Jason Allen

1991-01-01T23:59:59.000Z

270

Dynamic self-assembly in living systems as computation  

Science Conference Proceedings (OSTI)

ment. It is our view that much, if not all, of the business of a living system's building and maintaining itself is also a physical form of sto- chastic computing. 2.

271

Tracking Living Cells as They Differentiate in Real Time  

NLE Websites -- All DOE Office Websites (Extended Search)

Tracking Living Cells as They Tracking Living Cells as They Differentiate in Real Time Tracking Living Cells as They Differentiate in Real Time Print Thursday, 27 September 2012 00:00 Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible to watch phosphorylation at the molecular level without damaging cells or interfering with the very processes being examined. Using ALS Infrared Beamline 1.4.3, a group of researchers led by Hoi-Ying Holman, director of the Berkeley Synchrotron Infrared Structural Biology program at the ALS, developed a new technique for monitoring protein phosphorylation inside living mammalian cells, enabling them to follow cellular chemical changes in real time, without bias.

272

Living Direct: Proposed Penalty (2011-CE-1904) | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Proposed Penalty (2011-CE-1904) Proposed Penalty (2011-CE-1904) Living Direct: Proposed Penalty (2011-CE-1904) April 22, 2011 DOE alleged in a Notice of Proposed Civil Penalty that Living Direct, Inc. failed to certify a variety of dishwashers, refrigerator-freezers, and freezers as compliant with the applicable energy conservation standards. DOE regulations require a manufacturer (which includes importers) to submit reports certifying that its products have been tested and meet the applicable energy conservation standards. This civil penalty notice advises the company of the potential penalties and DOE's administrative process, including the company's right to a hearing. Living Direct: Proposed Penalty (2011-CE-1904) More Documents & Publications Living Direct: Order (2011-CE-1904)

273

Work of Manhattan Project-era photographer Ed Westcott lives...  

NLE Websites -- All DOE Office Websites (Extended Search)

Work of Manhattan ... Work of Manhattan Project-era photographer Ed Westcott lives on Posted: June 13, 2012 - 1:30pm Ed Westcott mans the shutter release for another historic...

274

Short RNA half-lives in the slow-growing marine cyanobacterium Prochlorococcus  

E-Print Network (OSTI)

Background: RNA turnover plays an important role in the gene regulation of microorganisms and influences their speed of acclimation to environmental changes. We investigated whole-genome RNA stability of Prochlorococcus, ...

Steglich, Claudia

275

Short RNA half-lives in the slow-growing marine cyanobacterium Prochlorococcus  

E-Print Network (OSTI)

Background RNA turnover plays an important role in the gene regulation of microorganisms and influences their speed of acclimation to environmental changes. We investigated whole-genome RNA stability of Prochlorococcus, a ...

Steglich, Claudia

276

Performance Requirements for Tools for Live Work on HVDC Systems  

Science Conference Proceedings (OSTI)

This report summarizes research on tools for live work (LW) that may be used for work on live high-voltage direct-current (HVDC) lines. There is very little detailed information on the performance of these tools when used on HVDC lines because these tools were initially developed for use on AC lines. This report is an account of EPRIs continuing research to fill this knowledge gap.BackgroundThe principles of DC LW and fiberglass reinforced ...

2012-12-14T23:59:59.000Z

277

Ladders of Insulating Material for Live Working: Research Plan  

Science Conference Proceedings (OSTI)

This report outlines a planned research and test program aimed at addressing concerns from field crews regarding undesirable mechanical behavior of insulating ladders used for live work, in particular regarding ladders that have been in service for some time. Long and spliced ladders can flex and twist, which could pose difficulties or safety issues to the worker on the ladder.BackgroundInsulating ladders for live work are made of insulating ...

2012-12-14T23:59:59.000Z

278

Geek-Up[09.24.10] -- Magical BEANs, Combating Bacteria's Resistance to  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

9.24.10] -- Magical BEANs, Combating Bacteria's Resistance 9.24.10] -- Magical BEANs, Combating Bacteria's Resistance to Antibiotics and the ChemCam's Journey to Mars Geek-Up[09.24.10] -- Magical BEANs, Combating Bacteria's Resistance to Antibiotics and the ChemCam's Journey to Mars September 24, 2010 - 5:19pm Addthis Check out the ChemCam close-up, which will reveal which elements are present in Mars' rocks and soils. Elizabeth Meckes Elizabeth Meckes Director of User Experience & Digital Technologies, Office of Public Affairs What are the key facts? BEANs -- phase-change materials -- have the ability to readily and swiftly transition between different phases, making them a valuable low-power source of flash memory and data storage. Scientists are studying E. coli bacteria's efflux pump to help them make inhibitors that will stop the heavy-metal pump and the antibiotic

279

Amoebae/bacteria consortia and uses for degrading wastes and contaminants  

DOE Patents (OSTI)

A method of altering trinitrotoluene includes the steps of: providing an amoeba/bacteria consortium, particularly ATCC 40908 or a mutant thereof possessing all the identifying characteristics thereof; and contacting the consortium with trinitrotoluene to alter the trinitrotoluene.

Tyndall, Richard L. (Clinton, TN)

1996-01-01T23:59:59.000Z

280

Controller parameter optimization for nonlinear systems using enhanced bacteria foraging algorithm  

Science Conference Proceedings (OSTI)

An enhanced bacteria foraging optimization (EBFO) algorithm-based Proportional + integral + derivative (PID) controller tuning is proposed for a class of nonlinear process models. The EBFO algorithm is a modified form of standard BFO algorithm. A multiobjective ...

V. Rajinikanth, K. Latha

2012-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

Geek-Up[12.03.2010]: Halomonadaceae Bacteria and the Return of Quark Gluon Plasma  

Energy.gov (U.S. Department of Energy (DOE))

The toxic element arsenic sustains growth of a bacteria instead of phosphorus and CERN's Collider gives researchers a look into the matter that may have existed in the very first moments of the universe.

282

Atomistic study of energy funneling in the light-harvesting complex of green sulfur bacteria  

E-Print Network (OSTI)

Phototrophic organisms such as plants, photosynthetic bacteria and algae use microscopic complexes of pigment molecules to absorb sunlight. Within the light-harvesting complexes, which frequently have multiple functional and structural subunits, the energy is transferred in the form of molecular excitations with very high efficiency. Green sulfur bacteria are considered to be amongst the most efficient light-harvesting organisms. Despite multiple experimental and theoretical studies of these bacteria the physical origin of the efficient and robust energy transfer in their light-harvesting complexes is not well understood. To study excitation dynamics at the systems level we introduce an atomistic model that mimic a complete light-harvesting apparatus of green sulfur bacteria. The model contains about 4000 pigment molecules and comprises a double wall roll for the chlorosome, a baseplate and six Fenna-Matthews-Olson trimer complexes. We show that the fast relaxation within functional subunits combined with the...

Huh, Joonsuk; Brookes, Jennifer C; Valleau, Stphanie; Fujita, Takatoshi; Aspuru-Guzik, Aln

2013-01-01T23:59:59.000Z

283

Response of Texas and Florida live oak (Quercus virginiana) seedlings to water deficit treatments.  

E-Print Network (OSTI)

??Live Oak (Quercus virginiana) is a common landscape tree in much of the United States. Although in native areas live oak can be found in (more)

Bonds, Amber Nicole

2007-01-01T23:59:59.000Z

284

Tracking Living Cells as They Differentiate in Real Time  

NLE Websites -- All DOE Office Websites (Extended Search)

Tracking Living Cells as They Differentiate in Real Time Print Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible to watch phosphorylation at the molecular level without damaging cells or interfering with the very processes being examined. Using ALS Infrared Beamline 1.4.3, a group of researchers led by Hoi-Ying Holman, director of the Berkeley Synchrotron Infrared Structural Biology program at the ALS, developed a new technique for monitoring protein phosphorylation inside living mammalian cells, enabling them to follow cellular chemical changes in real time, without bias.

285

Tracking Living Cells as They Differentiate in Real Time  

NLE Websites -- All DOE Office Websites (Extended Search)

Tracking Living Cells as They Differentiate in Real Time Print Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible to watch phosphorylation at the molecular level without damaging cells or interfering with the very processes being examined. Using ALS Infrared Beamline 1.4.3, a group of researchers led by Hoi-Ying Holman, director of the Berkeley Synchrotron Infrared Structural Biology program at the ALS, developed a new technique for monitoring protein phosphorylation inside living mammalian cells, enabling them to follow cellular chemical changes in real time, without bias.

286

Invisible Science: Lab Breakthroughs in Our Daily Lives | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Invisible Science: Lab Breakthroughs in Our Daily Lives Invisible Science: Lab Breakthroughs in Our Daily Lives Invisible Science: Lab Breakthroughs in Our Daily Lives April 24, 2012 - 2:30pm Addthis The Lab Breakthroughs video series focuses on the array of technological advancements and discoveries that stem from research performed in the National Labs, including improvements in industrial processes, discoveries in fundamental scientific research, and innovative medicines. See the Lab Breakthroughs topic page for the most recent videos and Q&As with researchers. The Lab Breakthroughs video series focuses on the array of technological advancements and discoveries that stem from research performed in the National Labs, including improvements in industrial processes, discoveries

287

Secretary Chu Speaks at Washington Post Live | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Chu Speaks at Washington Post Live Chu Speaks at Washington Post Live Secretary Chu Speaks at Washington Post Live November 3, 2011 - 9:12am Addthis Secretary Chu Secretary Chu Former Secretary of Energy Secretary Chu's remarks, as prepared for delivery, at the Washington Post Smart Energy Conference. Thank you, Mary [Jordan], for that kind introduction. While the focus of this conference is on the future of energy, I want to start with some lessons from America's past. On a windy day at Kitty Hawk on December 17, 1903, the Wright Brothers launched the world's first powered airplane to achieve human flight - and with it, a whole new industry. For the next several years, they led the world. What is less appreciated is that the United States lost the technology lead in airplanes by the beginning of World War I. Although the

288

Live Chat with the EERE Information Center | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Live Chat with the EERE Information Center Live Chat with the EERE Information Center Live Chat with the EERE Information Center December 6, 2010 - 6:30am Addthis Amy Foster Parish If you're an Energy Savers blog reader, hopefully you're also taking the opportunity to comment on the posts you read. Not only do the Energy Savers bloggers love to hear your feedback and answer your questions, commenting on the posts gives you a great opportunity to interact online with other folks who are really interested in energy efficiency and renewable energy. Heck, your comments might even make you famous if they end up in the Comment Spotlight as part of the blog's regular This Month on Energy Savers posts. What you might not know is that participating in the Energy Savers blog isn't the only way you can find help with your energy efficiency or

289

Secretary Chu Speaks at Washington Post Live | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Speaks at Washington Post Live Speaks at Washington Post Live Secretary Chu Speaks at Washington Post Live November 3, 2011 - 9:12am Addthis Secretary Chu Secretary Chu Former Secretary of Energy Secretary Chu's remarks, as prepared for delivery, at the Washington Post Smart Energy Conference. Thank you, Mary [Jordan], for that kind introduction. While the focus of this conference is on the future of energy, I want to start with some lessons from America's past. On a windy day at Kitty Hawk on December 17, 1903, the Wright Brothers launched the world's first powered airplane to achieve human flight - and with it, a whole new industry. For the next several years, they led the world. What is less appreciated is that the United States lost the technology lead in airplanes by the beginning of World War I. Although the

290

Greensburg, Kansas: A Better, Greener Place to Live (Revised) (Brochure) |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Greensburg, Kansas: A Better, Greener Place to Live (Revised) Greensburg, Kansas: A Better, Greener Place to Live (Revised) (Brochure) Greensburg, Kansas: A Better, Greener Place to Live (Revised) (Brochure) Content includes EERE subject matter and mentions DOE's role in rebuilding. The layout is designed to be used as a template for other cities in similar situations. 45086.pdf More Documents & Publications NREL Helps Greensburg Set the Model for Green Communities (Fact Sheet), Innovation: The Spectrum of Clean Energy Innovation, NREL (National Renewable Energy Laboratory) A Tale of Two Cities: Greensburg Rebuilds as a National Model for Green Communities (Fact Sheet), Energy Efficiency & Renewable Energy (EERE) Greensburg, Kansas: Building a Model Green Community, How Would You Rebuild a Town - Green? April 2009 (Brochure)

291

Greensburg, Kansas: A Better, Greener Place to Live (Revised) (Brochure) |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Greensburg, Kansas: A Better, Greener Place to Live (Revised) Greensburg, Kansas: A Better, Greener Place to Live (Revised) (Brochure) Greensburg, Kansas: A Better, Greener Place to Live (Revised) (Brochure) Content includes EERE subject matter and mentions DOE's role in rebuilding. The layout is designed to be used as a template for other cities in similar situations. 45086.pdf More Documents & Publications A Tale of Two Cities: Greensburg Rebuilds as a National Model for Green Communities (Fact Sheet), Energy Efficiency & Renewable Energy (EERE) NREL Helps Greensburg Set the Model for Green Communities (Fact Sheet), Innovation: The Spectrum of Clean Energy Innovation, NREL (National Renewable Energy Laboratory) Greensburg, Kansas: Building a Model Green Community, How Would You Rebuild a Town - Green? April 2009 (Brochure)

292

Interested in Open Energy Data? Watch the Energy Datapalooza Live! |  

NLE Websites -- All DOE Office Websites (Extended Search)

Interested in Open Energy Data? Watch the Energy Datapalooza Live! Interested in Open Energy Data? Watch the Energy Datapalooza Live! Energy Data Apps Maps Challenges Resources Blogs Let's Talk Energy Beta You are here Data.gov » Communities » Energy » Blogs Interested in Open Energy Data? Watch the Energy Datapalooza Live! Submitted by Matthew Loveless on Fri, 09/28/2012 - 5:28pm Log in to vote 14 On Monday, October 1st, more than 150 entrepreneurs, software developers, energy experts and policy makers are coming together for the first annual Energy Datapalooza. We'll be highlighting innovators that are using freely available data from the government and other sources to build products, services and apps that advance a secure and clean energy future. As a part of the Energy Data Initiative, the Datapalooza will also feature new datasets, application programming interfaces (APIs), and

293

Lab Breakthrough: How Energy Department Research Saves Lives | Department  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Lab Breakthrough: How Energy Department Research Saves Lives Lab Breakthrough: How Energy Department Research Saves Lives Lab Breakthrough: How Energy Department Research Saves Lives August 28, 2013 - 12:50pm Addthis Researchers at the National Energy Technology Lab have developed a platinum-chromium alloy that is used to make heart stents that are thin, flexible, corrosion resistant and visible on x-rays. Since their introduction in 2010, coronary stents made from the new alloy have generated more than $4 billion in worldwide sales and captured 45 percent of the U.S. coronary stent market and 33 percent of the global market, generating 450 high-paying, sustainable American jobs. | Video courtesy of National Energy Technology Laboratory. Renie Boyle Renie Boyle Public Affairs Specialist, National Energy Technology Laboratory

294

LIVE from the White House Science Fair | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

LIVE from the White House Science Fair LIVE from the White House Science Fair LIVE from the White House Science Fair October 18, 2010 - 11:31am Addthis John Schueler John Schueler Former New Media Specialist, Office of Public Affairs Editor's Note: This event has concluded. Today, Secretary Chu will join President Obama at the White House Science Fair to celebrate the winners of a broad range of science, technology, engineering and math (STEM) competitions. The winning student projects, which range from breakthrough basic research to new inventions, will be on display and the President will speak to students, science educators and business leaders in attendance about the importance of STEM education to our country's economic future. It's all part of the President's Educate to Innovate campaign, which

295

Talk to Secretary Chu Live This Wednesday | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Talk to Secretary Chu Live This Wednesday Talk to Secretary Chu Live This Wednesday Talk to Secretary Chu Live This Wednesday January 24, 2011 - 11:27am Addthis Ginny Simmons Ginny Simmons Former Managing Editor for Energy.gov, Office of Public Affairs After the State of the Union address this week, Secretary Steven Chu will host an online town hall to discuss President Obama's clean energy agenda. We hope you'll join us this Wednesday, January 26 at 12:45pm EST, at energy.gov/livechat. Two weeks ago, Secretary Chu asked what you most hoped to see the Department discussing. You responded with more topics than I have room to list, ranging from electric bicycles and LED lighting, to nuclear power, high-speed rail and energy independence. And so we're happy to say that Wednesday's town hall won't just be a single

296

Live Discussion on Energy 101: Fuel Cells | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Live Discussion on Energy 101: Fuel Cells Live Discussion on Energy 101: Fuel Cells Live Discussion on Energy 101: Fuel Cells January 16, 2014 - 3:59pm Addthis Rebecca Matulka Rebecca Matulka Digital Communications Specialist, Office of Public Affairs Editor's Note: Thanks to everyone who participated in our Google+ Hangout on Energy 101: Fuel Cells. We got a lot of great questions, and our experts talked about everything from the future of fuel cell vehicles and how they're being used as backup power to the efficiency benefits of fuel cells and how California is making fuel cell innovation a priority. If you missed the Hangout or want to check it out again, you can watch a recording of it above. Join us on Thursday, January 16, at 2 p.m. ET for Energy 101 -- the first in a new series of Google+ Hangouts about energy basics. Pulling together

297

Tracking Living Cells as They Differentiate in Real Time  

NLE Websites -- All DOE Office Websites (Extended Search)

Tracking Living Cells as They Differentiate in Real Time Print Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible to watch phosphorylation at the molecular level without damaging cells or interfering with the very processes being examined. Using ALS Infrared Beamline 1.4.3, a group of researchers led by Hoi-Ying Holman, director of the Berkeley Synchrotron Infrared Structural Biology program at the ALS, developed a new technique for monitoring protein phosphorylation inside living mammalian cells, enabling them to follow cellular chemical changes in real time, without bias.

298

Tracking Living Cells as They Differentiate in Real Time  

NLE Websites -- All DOE Office Websites (Extended Search)

Tracking Living Cells as They Differentiate in Real Time Print Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible to watch phosphorylation at the molecular level without damaging cells or interfering with the very processes being examined. Using ALS Infrared Beamline 1.4.3, a group of researchers led by Hoi-Ying Holman, director of the Berkeley Synchrotron Infrared Structural Biology program at the ALS, developed a new technique for monitoring protein phosphorylation inside living mammalian cells, enabling them to follow cellular chemical changes in real time, without bias.

299

LIVE: Meeting on Strengthening Deepwater Blowout Containment Capabilities |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

LIVE: Meeting on Strengthening Deepwater Blowout Containment LIVE: Meeting on Strengthening Deepwater Blowout Containment Capabilities LIVE: Meeting on Strengthening Deepwater Blowout Containment Capabilities September 22, 2010 - 12:56pm Addthis John Schueler John Schueler Former New Media Specialist, Office of Public Affairs At 1 PM EDT today Secretary Chu and Secretary of the Interior Ken Salazar will convene top U.S. government scientists and key industry and stakeholder leaders to discuss how to strengthen capabilities for responding to potential blowouts of oil and gas wells on the Outer Continental Shelf. The panel discussion will help guide reforms that are raising the bar for the oil and gas industry's practices, inform recommendations on whether and how to lift the current deepwater drilling suspension and assist in

300

Living Comfortably: A Consumer's Guide to Home Energy Upgrades |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Living Comfortably: A Consumer's Guide to Home Energy Upgrades Living Comfortably: A Consumer's Guide to Home Energy Upgrades Living Comfortably: A Consumer's Guide to Home Energy Upgrades March 7, 2013 - 3:15pm Addthis A weatherization worker drills holes to blow cellulose insulation in the interior walls of this home. | Photo courtesy of Dennis Schroeder, NREL A weatherization worker drills holes to blow cellulose insulation in the interior walls of this home. | Photo courtesy of Dennis Schroeder, NREL Dr. Richard Knaub Project Leader in Weatherization & Workforce Development at the National Renewable Energy Laboratory Energy audit tools An infrared camera can display temperature differences between surfaces and help determine if a wall is insulated. It can show drafts and moisture, which can lead to mold problems.

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

Green Light New York Releases Living Lab RFI  

NLE Websites -- All DOE Office Websites (Extended Search)

Green Light New York Releases Living Lab RFI Green Light New York Releases Living Lab RFI January 2014 Green Light New York and Lawrence Berkeley National Laboratory have partnered to develop a pair of "Living Lab" demonstration projects that will deploy innovative lighting, daylighting and shading systems in working New York City office spaces. The team has secured the participation of two Fortune 100 companies, each of which have committed to using an individual floor of their flagship NYC buildings as test beds for high performance systems. The project will include significant monitoring and analysis of the systems for a minimum of one year. Based on data collected and lessons learned over the course of the project the team will develop educational resources for the broader design, construction and real estate sectors,

302

Search for Charged Massive Long-Lived Particles  

SciTech Connect

We report on a search for charged massive long-lived particles (CMLLPs), based on 5.2 fb{sup -1} of integrated luminosity collected with the D0 detector at the Fermilab Tevatron p{bar p} collider. We search for events in which one or more particles are reconstructed as muons but have speed and ionization energy loss (dE/dx) inconsistent with muons produced in beam collisions. CMLLPs are predicted in several theories of physics beyond the standard model. We exclude pair-produced long-lived gaugino-like charginos below 267 GeV and Higgsino-like charginos below 217 GeV at 95% C.L., as well as long-lived scalar top quarks with mass below 285 GeV.

Abazov V. M.; Abbott B.; Acharya B. S.; Adams M.; Adams T.; Alexeev G. D.; Alimena J.; Alkhazov G.; Alton A.; Alverson G.; Alves G. A.; Aoki M.; Askew A.; Asman B.; Atkins S.; Atramentov O.; Augsten K.; Avila C.; BackusMayes J.; Badaud F.; Bagby L.; Baldin B.; Bandurin D. V.; Banerjee S.; Barberis E.; Baringer P.; Barreto J.; Bartlett J. F.; Bassler U.; Bazterra V.; Bean A.; Begalli M.; Belanger-Champagne C.; Bellantoni L.; Beri S. B.; Bernardi G.; Bernhard R.; Bertram I.; Besancon M.; Beuselinck R.; Bezzubov V. A.; Bhat P. C.; Bhatnagar V.; Blazey G.; Blessing S.; Bloom K.; Boehnlein A.; Boline D.; Boos E. E.; Borissov G.; Bose T.; Brandt A.; Brandt O.; Brock R.; Brooijmans G.; Bross A.; Brown D.; Brown J.; Bu X. B.; Buehler M.; Buescher V.; Bunichev V.; Burdin S.; Burnett T. H.; Buszello C. P.; Calpas B.; Camacho-Perez E.; Carrasco-Lizarraga M. A.; Casey B. C. K.; Castilla-Valdez H.; Chakrabarti S.; Chakraborty D.; Chan K. M.; Chandra A.; Chapon E.; Chen G.; Chevalier-Thery S.; Cho D. K.; Cho S. W.; Choi S.; Choudhary B.; Cihangir S.; Claes D.; Clutter J.; Cooke M.; Cooper W. E.; Corcoran M.; Couderc F.; Cousinou M. -C.; Croc A.; Cutts D.; Das A.; Davies G.; De K.; de Jong S. J.; De la Cruz-Burelo E.; Deliot F.; Demina R.; Denisov D.; Denisov S. P.; Desai S.; Deterre C.; DeVaughan K.; Diehl H. T.; Diesburg M.; Ding P. F.; Dominguez A.; Dorland T.; Dubey A.; Dudko L. V.; Duggan D.; Duperrin A.; Dutt S.; Dyshkant A.; Eads M.; Edmunds D.; Ellison J.; Elvira V. D.; Enari Y.; Evans H.; Evdokimov A.; Evdokimov V. N.; Facini G.; Ferbel T.; Fiedler F.; Filthaut F.; Fisher W.; Fisk H. E.; Fortner M.; Fox H.; Fuess S.; Garcia-Bellido A.; Garcia-Guerra G. A.; Gavrilov V.; Gay P.; Geng W.; Gerbaudo D.; Gerber C. E.; Gershtein Y.; Ginther G.; Golovanov G.; Goussiou A.; Grannis P. D.; Greder S.; Greenlee H.; Greenwood Z. D.; Gregores E. M.; Grenier G.; Gris Ph.; Grivaz J. -F.; Grohsjean A.; Gruenendahl S.; Gruenewald M. W.; Guillemin T.; Gutierrez G.; Gutierrez P.; Haas A.; Hagopian S.; Haley J.; Han L.; Harder K.; Harel A.; Hauptman J. M.; Hays J.; Head T.; Hebbeker T.; Hedin D.; Hegab H.; Heinson A. P.; Heintz U.; Hensel C.; Heredia-De La Cruz I.; Herner K.; Hesketh G.; Hildreth M. D.; Hirosky R.; Hoang T.; Hobbs J. D.; Hoeneisen B.; Hohlfeld M.; Hubacek Z.; Hynek V.; Iashvili I.; Ilchenko Y.; Illingworth R.; Ito A. S.; Jabeen S.; Jaffre M.; Jamin D.; Jayasinghe A.; Jesik R.; Johns K.; Johnson M.; Jonckheere A.; Jonsson P.; Joshi J.; Jung A. W.; Juste A.; Kaadze K.; Kajfasz E.; Karmanov D.; Kasper P. A.; Katsanos I.; Kehoe R.; Kermiche S.; Khalatyan N.; Khanov A.; Kharchilava A.; Kharzheev Y. N.; Kohli J. M.; Kozelov A. V.; Kraus J.; Kulikov S.; Kumar A.; Kupco A.; Kurca T.; Kuzmin V. A.; Kvita J.; Lammers S.; Landsberg G.; Lebrun P.; Lee H. S.; Lee S. W.; Lee W. M.; Lellouch J.; Li L.; Li Q. Z.; Lietti S. M.; Lim J. K.; Lincoln D.; Linnemann J.; Lipaev V. V.; Lipton R.; Liu Y.; Lobodenko A.; Lokajicek M.; de Sa R. Lopes; Lubatti H. J.; Luna-Garcia R.; Lyon A. L.; Maciel A. K. A.; Mackin D.; Madar R.; Magana-Villalba R.; Malik S.; Malyshev V. L.; Maravin Y.; Martinez-Ortega J.; McCarthy R.; McGivern C. L.; Meijer M. M.; et al.

2012-03-21T23:59:59.000Z

303

Live Chat with the EERE Information Center | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Live Chat with the EERE Information Center Live Chat with the EERE Information Center Live Chat with the EERE Information Center December 6, 2010 - 6:30am Addthis Amy Foster Parish If you're an Energy Savers blog reader, hopefully you're also taking the opportunity to comment on the posts you read. Not only do the Energy Savers bloggers love to hear your feedback and answer your questions, commenting on the posts gives you a great opportunity to interact online with other folks who are really interested in energy efficiency and renewable energy. Heck, your comments might even make you famous if they end up in the Comment Spotlight as part of the blog's regular This Month on Energy Savers posts. What you might not know is that participating in the Energy Savers blog isn't the only way you can find help with your energy efficiency or

304

Zombie Replicants to Outperform the Living | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Zombie Replicants to Outperform the Living Zombie Replicants to Outperform the Living Zombie Replicants to Outperform the Living February 8, 2013 - 5:08pm Addthis The first stage of the "zombie cell" only moderately heated, the cell is now pure silica and needed a gold coating for a scanning electron microscope to image it. | Photo courtesy of Sandia National Laboratories The first stage of the "zombie cell" only moderately heated, the cell is now pure silica and needed a gold coating for a scanning electron microscope to image it. | Photo courtesy of Sandia National Laboratories Michael Hess Michael Hess Former Digital Communications Specialist, Office of Public Affairs Is it just silica? Other porous material structures, relying on titanium instead of silica, have been formed using the organic template technique.

305

Public invited to share living with wildfire stories with BSM  

NLE Websites -- All DOE Office Websites (Extended Search)

June » June » Public invited to share living with wildfire stories with BSM Public invited to share living with wildfire stories with BSM The exhibit provides an opportunity for people to share their stories about the Las Conchas fire and other wildfires. June 11, 2012 Personal experiences and stories around wildfire are part of a new interactive exhibit at the Bradbury. Personal experiences and stories around wildfire are part of a new interactive exhibit at the Bradbury. Contact Steve Sandoval Communications Office (505) 665-9206 Email Interactive exhibit opens June 13 LOS ALAMOS, NEW MEXICO, June 11, 2012-Beginning June 13, the public is invited to Los Alamos National Laboratory's Bradbury Science Museum to take part in "Living With Wildfire: Share Your Stories." An opening

306

Table HC1.2.1. Living Space Characteristics by  

U.S. Energy Information Administration (EIA) Indexed Site

1. Living Space Characteristics by" 1. Living Space Characteristics by" " Total, Heated, and Cooled Floorspace, 2005" ,,,"Total Square Footage" ,"Housing Units",,"Total1",,"Heated",,"Cooled" "Living Space Characteristics","Millions","Percent","Billions","Percent","Billions","Percent","Billions","Percent" "Total",111.1,100,225.8,100,179.8,100,114.5,100 "Total Floorspace (Square Feet)1" "Fewer than 500",3.2,2.9,1.2,0.5,1.1,0.6,0.4,0.3 "500 to 999",23.8,21.4,17.5,7.7,15.9,8.8,7.3,6.4 "1,000 to 1,499",20.8,18.7,24.1,10.7,22.6,12.6,13,11.4 "1,500 to 1,999",15.4,13.9,24.5,10.9,22.2,12.4,14,12.2

307

Upcoming Live Training | ENERGY STAR Buildings & Plants  

NLE Websites -- All DOE Office Websites (Extended Search)

Upcoming Live Training Upcoming Live Training Secondary menu About us Press room Contact Us Portfolio Manager Login Facility owners and managers Existing buildings Commercial new construction Industrial energy management Small business Service providers Service and product providers Verify applications for ENERGY STAR certification Design commercial buildings Energy efficiency program administrators Commercial and industrial program sponsors Associations State and local governments Federal agencies Tools and resources Training In This Section Campaigns Commercial building design Communications resources Energy management guidance Financial resources Portfolio Manager Products and purchasing Recognition Research and reports Service and product provider (SPP) resources Success stories Target Finder Technical documentation

308

Genome sequence of the Fleming strain of Micrococcus luteus, a simple free- living actinobacterium  

SciTech Connect

Micrococcus luteus (NCTC2665, Fleming strain) has one of the smallest genomes of free living actinobacteria sequenced to date, comprising a single circular chromosome of 2,501,097 bp (G+C content 73%) predicted to encode 2403 proteins. The genome shows extensive synteny with that of the closely related organism, Kocuria rhizophila, from which it was taxonomically separated relatively recently. Despite its small size, the genome harbors 73 IS elements, almost all of which are closely related to elements found in other actinobacteria. An IS element is inserted into the rrs gene of one of only two rrn operons found in M. luteus. The genome encodes only four sigma factors and fourteen response regulators, indicative of adaptation to a rather strict ecological niche (mammalian skin). The high sensitivity of M. luteus to {Beta}-lactam antibiotics may result from the presence of a reduced set of penicillin binding proteins and the absence of a wblC gene, which plays an important role in antibiotic resistance in other actinobacteria. Consistent with the restricted range of compounds it can use as a sole source of carbon for energy and growth, M. luteus has a minimal complement of genes concerned with carbohydrate transport and metabolism and its inability to utilize glucose as a sole carbon source may be due to the apparent absence of a gene encoding glucokinase. Uniquely among characterized bacteria, M. luteus appears to be able to metabolize glycogen only via trehalose, and to make trehalose only via glycogen. It has very few genes associated with secondary metabolism. In contrast to other actinobacteria, M. luteus encodes only one resuscitation-promoting factor (Rpf) required for emergence from dormancy and its complement of other dormancy-related proteins is also much reduced. M. luteus is capable of long-chain alkene biosynthesis, which is of interest for advanced biofuel production; a three gene cluster essential for this metabolism has been identified in the genome.

Young, Michael; Artsatbanov, Vladislav; Beller, Harry R.; Chandra, Govind; Chater, Keith F.; Dover, Lynn G.; Goh, Ee-Been; Kahan, Tamar; Kaprelyants, Arseny S.; Kyrpides, Nikos; Lapidus, Alla; Lowry, Stephen R.; Lykidis, Athanasios; Mahillon, Jacques; Markowitz, Viktor; Mavrommatis, Konstantinos; Mukamolova, Galina V.; Oren, Aharon; Rokem, J. Stefan; Smith, Margaret C. M.; Young, Danielle I.; Greenblatt, Charles L.

2009-11-01T23:59:59.000Z

309

Turning Bacteria into Biofuel: Development of an Integrated Microbial Electrocatalytic (MEC) System for Liquid Biofuel Production from CO2  

Science Conference Proceedings (OSTI)

Electrofuels Project: LBNL is improving the natural ability of a common soil bacteria called Ralstonia eutropha to use hydrogen and carbon dioxide for biofuel production. First, LBNL is genetically modifying the bacteria to produce biofuel at higher concentrations. Then, LBNL is using renewable electricity obtained from solar, wind, or wave power to produce high amounts of hydrogen in the presence of the bacteriaincreasing the organisms access to its energy source and improving the efficiency of the biofuel-creation process. Finally, LBNL is tethering electrocatalysts to the bacterias surface which will further accelerate the rate at which the organism creates biofuel. LBNL is also developing a chemical method to transform the biofuel that the bacteria produce into ready-to-use jet fuel.

None

2010-08-01T23:59:59.000Z

310

Purple Bacteria Develops Its Own Form of Sunscreen | U.S. DOE Office of  

Office of Science (SC) Website

Purple Bacteria Develops Its Own Form Purple Bacteria Develops Its Own Form of "Sunscreen" Energy Frontier Research Centers (EFRCs) EFRCs Home Centers Research Science Highlights Highlight Archives News & Events Publications Contact BES Home 05.03.12 Purple Bacteria Develops Its Own Form of "Sunscreen" Print Text Size: A A A RSS Feeds FeedbackShare Page Scientific Achievement Found that specific pigments in the light harvesting complex of a photosynthetic bacterium act primarily to protect the cell from damage by excess sunlight Significance and Impact May aid the design of both natural and artificial light harvesting systems to minimize deleterious effects of exposure to too much light energy Research Details In photosynthetic organisms, carotenoids typically act as supplementary

311

Scientists Discover how Bacteria Convert Mercury to Toxic Form | U.S. DOE  

NLE Websites -- All DOE Office Websites (Extended Search)

Scientists Discover how Bacteria Convert Mercury to Toxic Form Scientists Discover how Bacteria Convert Mercury to Toxic Form Biological and Environmental Research (BER) BER Home About Research Facilities Science Highlights Searchable Archive of BER Highlights External link Benefits of BER Funding Opportunities Biological & Environmental Research Advisory Committee (BERAC) News & Resources Contact Information Biological and Environmental Research U.S. Department of Energy SC-23/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-3251 F: (301) 903-5051 E: sc.ber@science.doe.gov More Information » April 2013 Scientists Discover how Bacteria Convert Mercury to Toxic Form Two genes responsible for mercury methylation identified. Print Text Size: A A A Subscribe FeedbackShare Page Click to enlarge photo. Enlarge Photo

312

Liquid Fuel From Renewable Electricity and Bacteria: Electro-Autotrophic Synthesis of Higher Alcohols  

SciTech Connect

Electrofuels Project: UCLA is utilizing renewable electricity to power direct liquid fuel production in genetically engineered Ralstonia eutropha bacteria. UCLA is using renewable electricity to convert carbon dioxide into formic acid, a liquid soluble compound that delivers both carbon and energy to the bacteria. The bacteriaare genetically engineered to convert the formic acid into liquid fuelin this case alcohols such as butanol. The electricity required for the process can be generated from sunlight, wind, or other renewable energy sources. In fact, UCLAs electricity-to-fuel system could be a more efficient way to utilize these renewable energy sources considering the energy density of liquid fuel is much higher than the energy density of other renewable energy storage options, such as batteries.

2010-07-01T23:59:59.000Z

313

Living on the edge with the Oregon coastal atlas  

Science Conference Proceedings (OSTI)

In this paper, we describe an educational DVD entitled Living on the Edge: Building and Buying Property on the Oregon Coast, intended to alert homeowners, buyers, developers, realtors to the hazards associated with storms and other natural processes ... Keywords: atlas, coastal GIS, coastal resource management, geospatial data, internet map servers, natural hazards, public education, state government educational DVD, web GIS

Paul Klarin; Tanya Haddad; Joseph Cone; Dawn J. Wright

2006-05-01T23:59:59.000Z

314

Marshall Islands: a study of diet and living patterns  

SciTech Connect

This study summarizes information on diet and living patterns for the Marshallese. The data was derived from literature, answers to questionnaires, personal observations while living with the Marshallese for periods extending from months to years, and from direct participation in their activities. The results reflect the complex interactions of many influences, such as, the gathering of local foods the receipt of food aid through programs, such as, school-lunch, typhoon-relief, food distributed to populations displaced as a result of nuclear testing, and in recent times the availability of cash for the purchase of imported foods. The results identify these influences and are therefore restricted to local food diets while recognizing that the living patterns are changing as local food gathering is replaced by other food supplies. The data will therefore provide the necessary information for input into models that will assess the radiological impacts attributable to the inhabitation of the Marshall Islands. It is recommended that this study should be continued for at least two to three years in order to more accurately identify trends in local food consumption and living patterns.

Naidu, J.R.; Greenhouse, N.A.; Knight, G.; Craighead, E.C.

1980-07-01T23:59:59.000Z

315

Distribution of ranks of ?-decay half-lives  

E-Print Network (OSTI)

I studied the distribution of ranks of values of 2949 {\\beta}-decay half-lives according to an empirical beta law with two exponents. {\\beta}-decay half-life ranks showed good fit to a beta function with two exponents.

Juan Miguel Campanario

2010-11-21T23:59:59.000Z

316

Human Diversity: Our Genes Tell Where we Live  

E-Print Network (OSTI)

Human Diversity: Our Genes Tell Where we Live Dispatch Laurent Excoffier A detailed genetic. The novelty of the recent work of Rosenberg et al. [7] is precisely that they have checked the validity of the analysis of a cell-line panel of 52 worldwide popu- lations [9] managed by the French Center for the Study

Rosenberg, Noah

317

User modelling for live help systems: initial results  

Science Conference Proceedings (OSTI)

This paper explores the role of user modelling in live help systems for e-commerce web sites. There are several potential benefits with user modelling in this context: 1) Human assistants can use the personal information in the user models to provide ...

Johan Aberg; Nahid Shahmehri; Dennis Maciuszek

2001-10-01T23:59:59.000Z

318

LiveMail: personalized avatars for mobile entertainment  

Science Conference Proceedings (OSTI)

LiveMail is a prototype system that allows mobile subscribers to communicate using personalized 3D face models created from images taken by their phone cameras. The user takes a snapshot of someone's face - a friend, famous person, themselves, even a ...

Miran Mosmondor; Tomislav Kosutic; Igor S. Pandzic

2005-06-01T23:59:59.000Z

319

Turning Homes into Low-Cost Ambient Assisted Living Environments  

Science Conference Proceedings (OSTI)

Today motion recognition has become more popular in areas like health care. In real-time environments, the amount of information and data required to compute the user's motion is substantial, while the time to collect and process this information are ... Keywords: Ambient Assistant Living (AAL), Depth Image, Kinect Skeletal Data, Motion, Motion Recognition System

Alexiei Dingli; Daniel Attard; Ruben Mamo

2012-04-01T23:59:59.000Z

320

A lighting reproduction approach to live-action compositing  

Science Conference Proceedings (OSTI)

We describe a process for compositing a live performance of an actor into a virtual set wherein the actor is consistently illuminated by the virtual environment. The Light Stage used in this work is a two-meter sphere of inward-pointing RGB light emitting ... Keywords: global illumination, image-based lighting, matting and compositing, radiosity, reflectance and shading

Paul Debevec; Andreas Wenger; Chris Tchou; Andrew Gardner; Jamie Waese; Tim Hawkins

2002-07-01T23:59:59.000Z

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

Pervasive flexibility in living technologies through degeneracy-based design  

Science Conference Proceedings (OSTI)

The capacity to adapt can greatly influence the success of systems that need to compensate for damaged parts, learn how to achieve robust performance in new environments, or exploit novel opportunities that originate from new technological interfaces ... Keywords: Pervasive flexibility, adaptation, degeneracy, distributed robustness, living technologies

James Whitacre; Axel Bender

2013-10-01T23:59:59.000Z

322

Brazil's Right to Save Lives (NYT) 484 words  

E-Print Network (OSTI)

Brazil's Right to Save Lives (NYT) 484 words Published: June 23, 2005 Brazil has the best anti-name drugs. Brazil can freely copy any drug commercialized before 1997, when the country began to respect are still imported and are expensive, and Brazil is spending two-thirds of its antiretroviral budget on just

Lopez-Carr, David

323

Assembling living materials and engineering life-like technologies  

Science Conference Proceedings (OSTI)

Von Neumann, the inventor of the modern computer, realized that if life is a physical process, it should be possible to implement life in other media than biochemistry. In the 1950s, he was one of the first to propose the possibility of implementing ... Keywords: Chembio-ICT, living technology, minimal protocells, self-reproducing robots, sustainable personal fabricator network

Steen Rasmussen; Anders Albertsen; Harold Fellermann; Pernille Lykke Pedersen; Carsten Svaneborg; Hans Ziock

2011-07-01T23:59:59.000Z

324

Underwater Color Constancy : Enhancement of Automatic Live Fish Recognition  

E-Print Network (OSTI)

Underwater Color Constancy : Enhancement of Automatic Live Fish Recognition M. Chambah, D. Semani-mail: rizzi@dti.unimi.it ABSTRACT We present in this paper some advances in color restoration of underwater images, especially with regard to the strong and non uniform color cast which is typical of underwater

Paris-Sud XI, Université de

325

Safe and automatic live update for operating systems  

Science Conference Proceedings (OSTI)

Increasingly many systems have to run all the time with no downtime allowed. Consider, for example, systems controlling electric power plants and e-banking servers. Nevertheless, security patches and a constant stream of new operating system versions ... Keywords: automatic updates, live update, operating systems, state checking, state transfer, update safety

Cristiano Giuffrida; Anton Kuijsten; Andrew S. Tanenbaum

2013-04-01T23:59:59.000Z

326

Understanding the Role of the Bacteria, Isolated from the Hanford Site Soil, on the Fate and Transport of Uranium.  

E-Print Network (OSTI)

?? Bacteria are known for their abilities to influence the geochemical processes and affect the mobility of contaminants in the subsurface. Arthrobacter strain G975 was (more)

Carvajal, Denny A

2011-01-01T23:59:59.000Z

327

Genetically Modified Bacteria for Fuel Production: Development of Rhodobacteria as a Versatile Platform for Fuels Production  

SciTech Connect

Electrofuels Project: Penn State is genetically engineering bacteria called Rhodobacter to use electricity or electrically generated hydrogen to convert carbon dioxide into liquid fuels. Penn State is taking genes from oil-producing algae called Botryococcus braunii and putting them into Rhodobacter to produce hydrocarbon molecules, which closely resemble gasoline. Penn State is developing engineered tanks to support microbial fuel production and determining the most economical way to feed the electricity or hydrogen to the bacteria, including using renewable sources of power like solar energy.

None

2010-07-01T23:59:59.000Z

328

Genome sequence of the free-living aerobic spirochete Turneriella parva type strain (HT), and emendation of the species Turneriella parva  

Science Conference Proceedings (OSTI)

Turneriella parva Levett et al. 2005 is the only species of the genus Turneriella which was es- tablished as a result of the reclassification of Leptospira parva Hovind-Hougen et al. 1982. Together with Leptonema and Leptospira, Turneriella constitutes the family Leptospiraceae, within the order Spirochaetales. Here we describe the features of this free-living aerobic spi- rochete together with the complete genome sequence and annotation. This is the first com- plete genome sequence of a member of the genus Turneriella and the 13th member of the family Leptospiraceae for which a complete or draft genome sequence is now available. The 4,409,302 bp long genome with its 4,169 protein-coding and 45 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project.

Stackebrandt, Erko [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Chertkov, Olga [Los Alamos National Laboratory (LANL); Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Hammon, Nancy [U.S. Department of Energy, Joint Genome Institute; Deshpande, Shweta [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Huntemann, Marcel [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Pan, Chongle [ORNL; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Gronow, Sabine [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany

2013-01-01T23:59:59.000Z

329

Genomics Encyclopedia of Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB): a resource for microsymbiont genomes (2013 DOE JGI Genomics of Energy and Environment 8th Annual User Meeting)  

SciTech Connect

Wayne Reeve of Murdoch University on "Genomics Encyclopedia of Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB): a resource for microsymbiont genomes" at the 8th Annual Genomics of Energy & Environment Meeting on March 27, 2013 in Walnut Creek, Calif.

Reeve, Wayne [Murdoch University

2013-03-01T23:59:59.000Z

330

A method of genetically engineering acidophilic, heterotrophic, bacteria by electroporation and conjugation  

DOE Patents (OSTI)

A method of genetically manipulating an acidophilic bacteria is provided by two different procedures. Using electroporation, chimeric and broad-host range plasmids are introduced into Acidiphilium. Conjugation is also employed to introduce broad-host range plasmids into Acidiphilium at neutral pH.

Roberto, F.F.; Glenn, A.W.; Ward, T.E.

1990-08-07T23:59:59.000Z

331

Atomistic study of energy funneling in the light-harvesting complex of green sulfur bacteria  

E-Print Network (OSTI)

Phototrophic organisms such as plants, photosynthetic bacteria and algae use microscopic complexes of pigment molecules to absorb sunlight. Within the light-harvesting complexes, which frequently have multiple functional and structural subunits, the energy is transferred in the form of molecular excitations with very high efficiency. Green sulfur bacteria are considered to be amongst the most efficient light-harvesting organisms. Despite multiple experimental and theoretical studies of these bacteria the physical origin of the efficient and robust energy transfer in their light-harvesting complexes is not well understood. To study excitation dynamics at the systems level we introduce an atomistic model that mimic a complete light-harvesting apparatus of green sulfur bacteria. The model contains about 4000 pigment molecules and comprises a double wall roll for the chlorosome, a baseplate and six Fenna-Matthews-Olson trimer complexes. We show that the fast relaxation within functional subunits combined with the transfer between collective excited states of pigments can result in robust energy funneling that is weakly dependent on the initial excitation conditions and temperature changes. Moreover, the same mechanism describes the coexistence of multiple timescales of excitation dynamics frequently observed in ultrafast optical experiments. While our findings support the hypothesis of supertransfer, the model reveals energy transport through multiple channels on different length scales.

Joonsuk Huh; Semion K. Saikin; Jennifer C. Brookes; Stphanie Valleau; Takatoshi Fujita; Aln Aspuru-Guzik

2013-07-03T23:59:59.000Z

332

Dynamic estimation of specific growth rates and concentrations of bacteria for the anaerobic digestion  

E-Print Network (OSTI)

of the estimator performance. I. Introduction Anaerobic digestion is a biotechnological process with a promisingDynamic estimation of specific growth rates and concentrations of bacteria for the anaerobic digestion S. Diop1 and I. Simeonov2 Abstract-- The paper proposes an observability anal- ysis and estimation

333

Dynamic estimation of specific growth rates and concentrations of bacteria for the  

E-Print Network (OSTI)

for specific growth rates and biomass concentrations of the anaerobic digestion process. A 3-stage model of 5. INTRODUCTION Anaerobic digestion is a biotechnological process with a promising capabilities for solving someDynamic estimation of specific growth rates and concentrations of bacteria for the anaerobic

Paris-Sud XI, Université de

334

A dynamic estimation scheme of specific growth rates of bacteria for an anaerobic wastewater treatment process  

E-Print Network (OSTI)

. The dynamics of this process are the ones of standard anaerobic digestion, and depend on the type of organic is devoted to the description of the model of the specific anaerobic digestion processA dynamic estimation scheme of specific growth rates of bacteria for an anaerobic wastewater

335

Interactions of uranium with bacteria and kaolinite clay Toshihiko Ohnukia,*, Takahiro Yoshidaa  

E-Print Network (OSTI)

Interactions of uranium with bacteria and kaolinite clay Toshihiko Ohnukia,*, Takahiro Yoshidaa of uranium (VI) by a bacterium, Bacillus subtilis, suspended in a slurry of kaolinite clay, to elucidate, removed approximately 80% of the associated uranium. However, in the presence of B. subtilis the amount

Kasama, Takeshi

336

Live Oak County, Texas: Energy Resources | Open Energy Information  

Open Energy Info (EERE)

Live Oak County, Texas: Energy Resources Live Oak County, Texas: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 28.342294°, -98.0465185° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":28.342294,"lon":-98.0465185,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

337

Nuclear Science Day live webinar (National Nuclear Science Week) - Argonne  

NLE Websites -- All DOE Office Websites (Extended Search)

Nuclear Science Day live webinar Nuclear Science Day live webinar Search Go Home Postdocs Students Student Outreach Resources for Schools U.S.-based International (English) International (Other) Events IGED 2013 Science Careers in Search of Women Girls, choose a career in Nuclear Science and Technology! Argonne Nuclear Engineers tell why they chose a Nuclear Career Resources Contact Us Recent Events Science Careers in Search of Women, Apr. 18, 2013 Junior Girl Scout Workshop 'Atomic Fission Fun with the American Nuclear Society', Jan. 26, 2013 Getting to know nuclear energy: the past, the present & the future - free public lecture (Nov. 15, 2012, Argonne National Laboratory) On January 26, 2013, Argonne staff members participated in the Junior Girl Scout Workshop 'Atomic Fission Fun with the American Nuclear Society'

338

Greensburg, Kansas: A Better, Greener Place to Live (Revised) (Brochure)  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

biggest success story in biggest success story in Greensburg, to me, has been the resiliency and determination of our citizens to make a difference in their world. We're new pioneers in the sustainability movement." - Greensburg Mayor Bob Dixson There's No Place Like Home G reensburg, Kansas is Midwestern farm country. Its 900 residents are hard- working people who love their home and their way of life. They simply will not give up when it comes to making their community a better place to live. After the town was nearly wiped out by a massive tornado in May 2007, citizens saw the opportu- nity to make Greensburg something even better than it had been before. Living close to the land, they knew the value of solar and wind power

339

Live from the Clinton Global Initiative | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

from the Clinton Global Initiative from the Clinton Global Initiative Live from the Clinton Global Initiative September 21, 2010 - 1:00pm Addthis John Schueler John Schueler Former New Media Specialist, Office of Public Affairs What does this project do? We're partnering with the Clinton Global Initiative and other NGOs to bring clean-burning cookstoves to the developing world. Today Secretary of State Hillary Rodham Clinton formally announced the Global Alliance for Clean Cookstoves, a new public-private initiative to create a thriving global market for clean and efficient household cooking solutions that will save lives, improve livelihoods and combat climate change. Secretary Clinton outlined partnership and financial commitments of the Alliance as part of the Clinton Global Initiative's annual meeting.

340

Tropical Africa: Calculated Actual Aboveground Live Biomass in Open and  

NLE Websites -- All DOE Office Websites (Extended Search)

Calculated Actual Aboveground Live Biomass in Open and Calculated Actual Aboveground Live Biomass in Open and Closed Forests (1980) image Brown, S., and G. Gaston. 1996. Tropical Africa: Land Use, Biomass, and Carbon Estimates For 1980. ORNL/CDIAC-92, NDP-055. Carbon Dioxide Information Analysis Center, U.S. Department of Energy, Oak Ridge National Laboratory, Oak Ridge, Tennessee, U.S.A. More Maps Land Use Maximum Potential Biomass Density Area of Closed Forests (By Country) Mean Biomass of Closed Forests (By Country) Area of Open Forests (By Country) Mean Biomass of Open Forests (By County) Percent Forest Cover (By Country) Total Forest Biomass (By Country) Population Density - 1990 (By Administrative Unit) Population Density - 1980 (By Administrative Unit) Population Density - 1970 (By Administrative Unit) Population Density - 1960 (By Administrative Unit)

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


341

NETL: News Release - Robot Successfully Inspects Live Natural Gas Pipeline  

NLE Websites -- All DOE Office Websites (Extended Search)

22, 2004 22, 2004 Robot Successfully Inspects Live Natural Gas Pipeline in New York Field Test is a First for Natural Gas Industry BROCKPORT, NY - In a recent field demonstration filled with "firsts," a self-powered robot developed by the Northeast Gas Association, Carnegie Mellon University, and the Department of Energy's National Energy Technology Laboratory successfully inspected a mile of a live natural gas distribution main in Brockport, New York. Known as EXPLORER, the remote-controlled robot was launched and retrieved four times on October 8 with no interruption in customer service. The system successfully made its way through an 8-inch diameter pipeline owned and operated by Rochester Electric & Gas, and maneuvered several 70- to 90-degree bends.

342

MHK Projects/Live Oak Project | Open Energy Information  

Open Energy Info (EERE)

Live Oak Project Live Oak Project < MHK Projects Jump to: navigation, search << Return to the MHK database homepage Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":5,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"500px","height":"350px","centre":false,"title":"","label":"","icon":"File:Aquamarine-marker.png","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":29.7638,"lon":-90.0278,"alt":0,"address":"","icon":"http:\/\/prod-http-80-800498448.us-east-1.elb.amazonaws.com\/w\/images\/7\/74\/Aquamarine-marker.png","group":"","inlineLabel":"","visitedicon":""}]}

343

DEEP RESIDENTIAL RETROFITS - USING LESS AND LIVING BETTER  

NLE Websites -- All DOE Office Websites (Extended Search)

DEEP RESIDENTIAL RETROFITS - USING LESS AND LIVING BETTER DEEP RESIDENTIAL RETROFITS - USING LESS AND LIVING BETTER Speaker(s): Iain Walker Date: December 11, 2009 - 12:00pm Location: 90-3122 (This is a repeat of the Nov. 18 ME EET Seminar on campus) There are currently thousands of federal, state and utility programs starting up throughout he nation to retrofit existing homes. Most of these programs have moderate savings goals on the order of 20%, but to really make an impact and make the nations housing stock sustainable we need savings of 70% or more. This requires fundamental changes in the way we think about retrofits. We need better diagnostics to determine how houses perform - both before and after retrofitting, we need better ways of simulating home performance so we can make better decisions about what to do to a home to

344

Electric and Magnetic Field Exposure During Live Line Work  

Science Conference Proceedings (OSTI)

An employee performing work on a transmission line while that line is energized is subjected to the local electric and magnetic fields produced by the voltage and current on the line. The electric field can result in spark discharges, contact currents, and stimulation of the body hair with attendant annoyance. Magnetic fields do not result in shock phenomena, but have become of concern to some people with regard to alleged health effects. Magnetic field shielding is not practical for live line ...

2013-10-07T23:59:59.000Z

345

Field Guide: Live Working Rope (Optimized for Electronic Viewing)  

Science Conference Proceedings (OSTI)

Ropes are an essential tool in many applications associated with the electric power industry. Ropes used in proximity to or in contact with high-voltage power lines require demanding dielectric properties as well as strength and durability. A comprehensive understanding of live working rope use is necessary for safe and efficient operations wherever such ropes are used. This field guide has been optimized for viewing on electronic devices. For a copy of this product printed on high-quality paper and ring...

2011-12-21T23:59:59.000Z

346

Hanwha L C Hanwha Living and Creative | Open Energy Information  

Open Energy Info (EERE)

Hanwha L C Hanwha Living and Creative Hanwha L C Hanwha Living and Creative Jump to: navigation, search Name Hanwha L&C (Hanwha Living and Creative) Place Seoul, Seoul, Korea (Republic) Zip 100-797 Sector Solar Product Seoul-based construction materials company. The company is also a producer of solar EVA laminates. Coordinates 37.557121°, 126.977379° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":37.557121,"lon":126.977379,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

347

Arsenic exposure in children living near a former copper smelter  

SciTech Connect

About 10,000 people live in communities surrounding the former copper smelter at Anaconda, Montana. Most of these people live in the town of Anaconda, which is generally upwind of the smelter. The smelter ceased operations in 1980, after almost a century of ore processing. Soil and dust on the smelter site and in the vicinity remain contaminated with arsenic, although at this time air and drinking water arsenic levels are not elevated. Results of soil and dust sampling for arsenic in the communities around the smelter are reported. In the town of Anaconda, surface soil arsenic levels from residential sites have averaged around 100 ppm or greater. Young children are generally believed to be the population with the most nonoccupational exposure to soil. Several models of exposure to environmental arsenic in the Anaconda area have predicted that children living in all communities surrounding the smelter would be having significant and measurable exposure to arsenic. Two exposures surveys, conducted while the smelter was operative, demonstrated that excess exposure to arsenic was occurring in young children. Until the present surveys, no exposure data had been collected since the smelter was closed.

Binder, S.; Forney, D.; Kaye, W.; Paschal, D.

1987-07-01T23:59:59.000Z

348

Plutonium(V/VI) Reduction by the Metal-Reducing Bacteria  

E-Print Network (OSTI)

We examined the ability of the metal-reducing bacteria Geobacter metallireducens GS-15 and Shewanella oneidensis MR-1 to reduce Pu(VI) and Pu(V). Cell suspensions of both bacteria reduced oxidized Pu [a mixture of Pu(VI) and Pu(V)] to Pu(IV). The rate of plutonium reduction was similar to the rate of U(VI) reduction obtained under similar conditions for each bacteria. The rates of Pu(VI) and U(VI) reduction by cell suspensions of S. oneidensis were slightly higher than the rates observed with G. metallireducens. The reduced form of Pu was characterized as aggregates of nanoparticulates of Pu(IV). Transmission electron microscopy images of the solids obtained from the cultures after the reduction of Pu(VI) and Pu(V) by S. oneidensis show that the Pu precipitates have a crystalline structure. The nanoparticulates of Pu(IV) were precipitated on the surface of or within the cell walls of the bacteria. The production of Pu(III) was not observed, which indicates that Pu(IV) was the stable form of reduced Pu under these experimental conditions. Experiments examining the ability of these bacteria to use Pu(VI) as a terminal electron acceptor for growth were inconclusive. A slight increase in cell density was observed for both G. metallireducens and S. oneidensis when Pu(VI) was provided as the sole electron acceptor; however, Pu(VI) concentrations decreased similarly in both the experimental and control cultures. Effective bioremediation and waste management strategies

Gary A. Icopini; Joe G. Lack; Larry E. Hersman; Mary P. Neu; Hakim Boukhalfa

2009-01-01T23:59:59.000Z

349

Physiological diversity and distributions of heterotrophic bacteria in deep cretaceous sediments of the Atlantic coastal plain  

SciTech Connect

A series of 23 intact core segments was obtained from two distinct deep subsurface geological formations, the Middendorf and the Cape Fear formations, underlying the southeastern coastal plain of South Carolina. Aerobic chemoheterotrophic bacteria were enumerated on a dilute medium, and populations ranged from 3.1 to 6.4 log CFU g of sediment[sup [minus]1] in the Middendorf cores and from below detection to 4.3 log CFU g[sup [minus]1] in the Cape Fear cores. A total of 198 morphologically distinct colony types were isolated, purified, and subjected to 108 different physiological measurements. The isolates from the two formations were distinct as were those in different core samples from the same formation. Cluster analysis revealed 21 different biotypes based on similarities of 75% or higher in response patterns to 21 physiological assays. One biotype contained 57 of the subsurface isolates, 10 biotypes contained 5 or more isolates, and the remainder had 4 or fewer. The organic compounds that were most commonly metabolized by the subsurface bacteria included Tween 40 and [beta]-hydroxybutyric acid. Organic acids, in general, were also commonly metabolized by the subsurface bacteria. Isolates from the Cape Fear core segments were capable of metabolizing a higher percentage of the substrates than were bacteria isolated from the Middendorf formation. Although the heterogeneous distributions of bacteria in deep subsurface sediments may make it difficult to use aquifer microcosms to predict in situ biotransformation rates, the diversity of the physiological properties of these organisms offers promise for in situ remediation of contaminants.

Fredrickson, J.K.; Zachara, J.M.; Li, S.W.; Brockman, F.J.; Simmons, M.A. (Pacific Northwest Lab., Richland, WA (United States)); Balkwill, D.L. (Florida State Univ., Tallahassee (United States))

1991-02-01T23:59:59.000Z

350

Lvy Fluctuations and Tracer Diffusion in Dilute Suspensions of Algae and Bacteria  

E-Print Network (OSTI)

Swimming microorganisms rely on effective mixing strategies to achieve efficient nutrient influx. Recent experiments, probing the mixing capability of unicellular biflagellates, revealed that passive tracer particles exhibit anomalous non-Gaussian diffusion when immersed in a dilute suspension of self-motile Chlamydomonas reinhardtii algae. Qualitatively, this observation can be explained by the fact that the algae induce a fluid flow that may occasionally accelerate the colloidal tracers to relatively large velocities. A satisfactory quantitative theory of enhanced mixing in dilute active suspensions, however, is lacking at present. In particular, it is unclear how non-Gaussian signatures in the tracers' position distribution are linked to the self-propulsion mechanism of a microorganism. Here, we develop a systematic theoretical description of anomalous tracer diffusion in active suspensions, based on a simplified tracer-swimmer interaction model that captures the typical distance scaling of a microswimmer's flow field. We show that the experimentally observed non-Gaussian tails are generic and arise due to a combination of truncated L\\'evy statistics for the velocity field and algebraically decaying time correlations in the fluid. Our analytical considerations are illustrated through extensive simulations, implemented on graphics processing units to achieve the large sample sizes required for analyzing the tails of the tracer distributions.

Irwin M. Zaid; Jrn Dunkel; Julia M. Yeomans

2010-09-20T23:59:59.000Z

351

ORGANIC GEOCHEMICAL STUDIES. II. THE DISTRIBUTION OF ALIPHATIC HYDROCARBONS IN ALGAE, BACTERIA, AND IN A RECENT LAKE SEDIMENT: A PRELIMINARY REPORT  

E-Print Network (OSTI)

ALIPHATIC HYDROCARBONS IN ALGAE, BACTERIA, AND IN A RECENTH F A PRELIMINARY REPORT IN ALGAE, BACTERIA, AKD IN A RECENTrests on the finding that algae have less cellulose and a

Han, Jerry; McCarthy, E.D.; Van Hoeven Jr., William; Calvin, Melvin; Bradley, W. H.

2008-01-01T23:59:59.000Z

352

Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals  

DOE Patents (OSTI)

Novel double and triple fusion reporter gene constructs harboring distinct imagable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

Gambhir, Sanjiv (Portola Valley, CA); Pritha, Ray (Mountain View, CA)

2011-06-07T23:59:59.000Z

353

ScienceLive chat page: on the future of fusion research | Princeton...  

NLE Websites -- All DOE Office Websites (Extended Search)

ScienceLive chat page: on the future of fusion research American Fusion News Category: U.S. Universities Link: ScienceLive chat page: on the future of fusion research...

354

Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals  

SciTech Connect

Novel double and triple fusion reporter gene constructs harboring distinct imageable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

Gambhir; Sanjiv (Portola Valley, CA), Pritha; Ray (Mountain View, CA)

2009-04-28T23:59:59.000Z

355

Dysfunction of Organic Anion Transporting Polypeptide 1a1 Alters Intestinal Bacteria and Bile Acid Metabolism in Mice  

E-Print Network (OSTI)

Organic anion transporting polypeptide 1a1 (Oatp1a1) is predominantly expressed in liver and is able to transport bile acids (BAs) in vitro. Male Oatp1a1-null mice have increased concentrations of taurodeoxycholic acid (TDCA), a secondary BA generated by intestinal bacteria, in both serum and livers. Therefore, in the present study, BA concentrations and intestinal bacteria in wild-type (WT) and Oatp1a1-null mice were quantified to investigate whether the increase of secondary BAs in Oatp1a1-null mice is due to alterations in intestinal bacteria. The data demonstrate that Oatp1a1-null mice: (1) have similar bile flow and BA concentrations in bile as WT mice; (2) have a markedly different BA composition in the intestinal contents, with a decrease in conjugated BAs and an increase in unconjugated BAs; (3) have BAs in the feces that are more deconjugated, desulfated, 7-dehydroxylated, 3-epimerized, and oxidized, but less 7-epimerized; (4) have 10-fold more bacteria in the small intestine, and 2-fold more bacteria in the large intestine which is majorly due to a 200 % increase in Bacteroides and a 30 % reduction in Firmicutes; and (5) have a different urinary excretion of bacteria-related metabolites than WT mice. In conclusion, the present study for the first time established that lack of a liver transporter (Oatp1a1) markedly alters the intestinal environment in mice, namely the bacteria composition.

Youcai Zhang; Pallavi B. Limaye; Lois D. Lehman-mckeeman; Curtis D. Klaassen

2011-01-01T23:59:59.000Z

356

EPRI Live Working Project: Summaries of Published Reports 1983-2012 and Listing of Presentations at EPRI and Other Live Working Conferences  

Science Conference Proceedings (OSTI)

EPRI has been conducting research in the area of live working for several decades. This research has resulted in a large number of reports and other products. To help users locate the results of EPRIs research in live working, this annual update provides brief descriptions of relevant EPRI products.BackgroundLive work the performance of maintenance, construction, or testing on equipment and circuits that are energized or that may become ...

2013-08-12T23:59:59.000Z

357

Station Living Program, RCM, and the maintenance rule  

Science Conference Proceedings (OSTI)

A project is being conducted at Boston Edison's Pilgrim nuclear power station (PNPS) to develop, implement, and demonstrate the effectiveness of a Station Living Program and the US Nuclear Regulatory Commission (NRC) maintenance rule. This project is a collaborative effort by PNPS, the Electric Power Corp, and Quadrex Energy Series Corporation. In parallel, a preventive maintenance optimization project utilizing reliability-centered maintenance (RCM) is being performed on [approximately]90% of the plant's systems. These two projects are being combined as a major cornerstone at PNPS in the implementation of the NRC maintenance rule.

Kleam, J. (Boston Edison Co., Plymouth, MA (United States)); Anderson, J. (Quadrex Energy Services Corp., Campbell, CA (United States))

1993-01-01T23:59:59.000Z

358

Turning Bacteria into Fuel: Cyanobacteria Designed for Solar-Powered Highly Efficient Production of Biofuels  

SciTech Connect

Broad Funding Opportunity Announcement Project: ASU is engineering a type of photosynthetic bacteria that efficiently produce fatty acidsa fuel precursor for biofuels. This type of bacteria, called Synechocystis, is already good at converting solar energy and carbon dioxide (CO2) into a type of fatty acid called lauric acid. ASU has modified the organism so it continuously converts sunlight and CO2 into fatty acidsoverriding its natural tendency to use solar energy solely for cell growth and maximizing the solar-to-fuel conversion process. ASUs approach is different because most biofuels research focuses on increasing cellular biomass and not on excreting fatty acids. The project has also identified a unique way to convert the harvested lauric acid into a fuel that can be easily blended with existing transportation fuels.

2010-01-01T23:59:59.000Z

359

Printed in Great Britain 107 The Decomposition of Toluene by Soil Bacteria  

E-Print Network (OSTI)

Strains of two bacteria, a Pseudomonas and an Achromobacter, which grow with toluene, benzene or certain other related aromatic compounds as sole carbon source were isolated from soil. The use of aromatic compounds by these bacteria was an induced phenomenon. Toluene-grown organisms oxidized without lag toluene, benzene, catechol, &methylcatechol, benzyl alcohol and, more slowly, o- and m-cresol, but not benzaldehyde or benzoic acid. 3-Methylcatechol, acetic acid, pyruvic acid, and a yellow ether-soluble acidic substance which was colourless in acid solution, were detected in toluene-oxidizing cultures. Acetic and pyruvic acids were also formed during the bacterial oxidation of 8-methylcatechol. 3-Methylcatechol is probably an early stage in the bacterial metabolism of toluene; benzaldehyde and benzoic acid seem not to be intermediates in this metabolism.

D. Claus

1964-01-01T23:59:59.000Z

360

Intelligent Transportation Systems: Saving Lives, Time and Money Portland State University  

E-Print Network (OSTI)

Intelligent Transportation Systems: Saving Lives, Time and Money Portland State University Oct 14, 2009 #12;Intelligent Transportation Systems: Saving Lives, Time and Money James Whiteneck #12;Intelligent Transportation Systems: Saving Lives, Time and Money Portal archives a large amount of data Over

Bertini, Robert L.

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


361

1Intelligent Transportation Systems: Saving Lives, Time and Money PORTAL Advisory Committee  

E-Print Network (OSTI)

1Intelligent Transportation Systems: Saving Lives, Time and Money PORTAL Advisory Committee Initial Meeting January 22, 2009 #12;2Intelligent Transportation Systems: Saving Lives, Time and Money Agenda 9:00 Adjourn #12;3Intelligent Transportation Systems: Saving Lives, Time and Money What's in the PORTAL

Bertini, Robert L.

362

A review on vision techniques applied to Human Behaviour Analysis for Ambient-Assisted Living  

Science Conference Proceedings (OSTI)

Human Behaviour Analysis (HBA) is more and more being of interest for computer vision and artificial intelligence researchers. Its main application areas, like Video Surveillance and Ambient-Assisted Living (AAL), have been in great demand in recent ... Keywords: Action recognition, Activities of daily living (ADLs), Activity recognition, Ambient-Assisted Living, Computer vision, Human behaviour, Motion analysis

Alexandros Andr Chaaraoui; Pau Climent-Prez; Francisco Flrez-Revuelta

2012-09-01T23:59:59.000Z

363

Clay enhancement of methane, low molecular weight hydrocarbon and halocarbon conversion by methanotrophic bacteria  

DOE Patents (OSTI)

The invention described in this report relates to a combined system of an apparatus and a method of increasing the rates of oxidation of gases and hazardous vapors by methanotrophic and other bacteria. The gases of interest are methane and trichlorethylene and other hazardous vapors. In a preferred embodiment, the oxidation rate of methane is improved by the addition of clays, e.g., kaolin, sometimes called ``China clay.``

Apel, W.A.; Dugan, P.R.

1991-12-31T23:59:59.000Z

364

A Scanning Auger Microprobe analysis of corrosion products associated with sulfate reducing bacteria  

Science Conference Proceedings (OSTI)

A Scanning Auger Microprobe analysis was performed on the corrosion products of an austenitic AISI type 304 SS after a potentiostatic polarization of one volt for ten minutes in a modified Postgate`s C media containing sulfate reducing bacteria. The corrosion products were characterized and mapped in local regions where pitting was observed. A critical evaluation of the applicability of this technique for the examination of microbially influenced corrosion (MIC) is presented.

Sadowski, R.A.; Chen, G.; Clayton, C.R.; Kearns, J.R. [State Univ. of New York, Stony Brook, NY (United States). Dept. of Materials Science and Engineering; Gillow, J.B.; Francis, A.J. [Brookhaven National Lab., Upton, NY (United States). Biosystems and Process Sciences Div.

1995-03-01T23:59:59.000Z

365

Diffusive transport without detailed balance in motile bacteria: Does microbiology need statistical physics?  

E-Print Network (OSTI)

Microbiology is the science of microbes, particularly bacteria. Many bacteria are motile: they are capable of self-propulsion. Among these, a significant class execute so-called run-and-tumble motion: they follow a fairly straight path for a certain distance, then abruptly change direction before repeating the process. This dynamics has something in common with Brownian motion (it is diffusive at large scales), and also something in contrast. Specifically, motility parameters such as the run speed and tumble rate depend on the local environment and hence can vary in space. When they do so, even if a steady state is reached, this is not generally invariant under time-reversal: the principle of detailed balance, which restores the microscopic time-reversal symmetry of systems in thermal equilibrium, is mesoscopically absent in motile bacteria. This lack of detailed balance (allowed by the flux of chemical energy that drives motility) creates pitfalls for the unwary modeller. Here I review some statistical mechanical models for bacterial motility, presenting them as a paradigm for exploring diffusion without detailed balance. I also discuss the extent to which statistical physics is useful in understanding real or potential microbiological experiments.

M. E. Cates

2012-08-20T23:59:59.000Z

366

Design, testing and optimization of a microfluidic device for capture and concentration of bacteria  

E-Print Network (OSTI)

Effective detection of bacterial pathogens in large sample volumes is a challenging problem. Pre-concentration routines currently in practice before the actual detection process are cumbersome and hard to automate. An effort is made to address the problem of volume discrepancy between day-to-day samples and the concentrated samples needed for analysis. Principles of conceptual design are used in formulating the â??Need Statementâ??, â??Function Structureâ?? and in identifying the â??Critical Design Parametersâ?? and â??Design Constraintsâ??. Electrokinetic phenomena are used to exploit the surface charges on bacteria. Electrophoresis is used to transport the bacteria to electrode surface and â??Electrostatic trappingâ? is then used to capture these microbes on the electrode surface. The captured microbes can then be concentrated in a concentrator unit. A prototype microfluidic device is fabricated for showing the proof of concept. Optimization is done to minimize hydraulic power consumption and wetted volume. Observations from the initial prototype device along with the optimization results are used in building a new prototype device. Operation of this device is demonstrated by capture of bacteria from flow. Qualitative studies are conducted and preliminary quantification is also done.

Cherla, Srinivas

2005-08-01T23:59:59.000Z

367

Reprogramming Bacteria to Seek and Destroy Small Molecules (JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)  

Science Conference Proceedings (OSTI)

Justin Gallivan, of Emory University presents a talk titled "Reprogramming Bacteria to Seek and Destroy Small Molecules" at the JGI User 7th Annual Genomics of Energy & Environment Meeting on March 21, 2012 in Walnut Creek, Calif

Gallivan, Justin [Emory University

2012-03-21T23:59:59.000Z

368

The effects of temperature and phosphorus availability on the biomass composition, phosphorus allocation, size and morphology of freshwater bacteria.  

E-Print Network (OSTI)

??University of Minnesota Ph.D. dissertation. July 2012. Major: Ecology, Evolution and Behavior. Advisor:James Bryan Cotner. 1 computer file (PDF); iv, 112 pages. Freshwater bacteria play (more)

Phillips, Katherine N.

2012-01-01T23:59:59.000Z

369

Liquid Fuel From Bacteria: Engineering Ralstonia eutropha for Production of Isobutanol (IBT) Motor Fuel from CO2, Hydrogen, and Oxygen  

Science Conference Proceedings (OSTI)

Electrofuels Project: MIT is using solar-derived hydrogen and common soil bacteria called Ralstonia eutropha to turn carbon dioxide (CO2) directly into biofuel. This bacteria already has the natural ability to use hydrogen and CO2 for growth. MIT is engineering the bacteria to use hydrogen to convert CO2 directly into liquid transportation fuels. Hydrogen is a flammable gas, so the MIT team is building an innovative reactor system that will safely house the bacteria and gas mixture during the fuel-creation process. The system will pump in precise mixtures of hydrogen, oxygen, and CO2, and the online fuel-recovery system will continuously capture and remove the biofuel product.

None

2010-07-15T23:59:59.000Z

370

Single Cell Oils: Microbial and Algal Oils, 2nd EditionChapter 14 Production of Lipids for Biofuels Using Bacteria  

Science Conference Proceedings (OSTI)

Single Cell Oils: Microbial and Algal Oils, 2nd Edition Chapter 14 Production of Lipids for Biofuels Using Bacteria Biofuels and Bioproducts and Biodiesel Biofuels - Bioproducts eChapters Press Downloadable pdf of

371

TOMORROW: Secretary Chu To Host Earth Day Live Chat | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Secretary Chu To Host Earth Day Live Chat Secretary Chu To Host Earth Day Live Chat TOMORROW: Secretary Chu To Host Earth Day Live Chat April 19, 2012 - 12:10pm Addthis WASHINGTON, DC - On Friday, April 20, 2012, U.S. Energy Secretary Steven Chu will host a livechat as part of the Energy Department's Earth Day celebrations to highlight the environmental and economic benefits of the Department's investments in clean energy. The live chat will be streamed on www.energy.gov tomorrow, and will feature questions from both in-person and online audiences. WHAT: Energy Secretary Steven Chu to Host Earth Day Live Chat WHEN: Friday, April 20, 2012 10:45 AM EDT NOTE: The livechat by Secretary Chu will be streamed online at 10:45 AM at www.energy.gov/live. Addthis Related Articles TOMORROW: Secretary Chu To Host Earth Day Live Chat

372

WATCH LIVE: Talking the Higgs Boson with CERN | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

WATCH LIVE: Talking the Higgs Boson with CERN WATCH LIVE: Talking the Higgs Boson with CERN WATCH LIVE: Talking the Higgs Boson with CERN September 13, 2012 - 2:33pm Addthis WATCH LIVE: Talking the Higgs Boson with CERN Michael Hess Michael Hess Former Digital Communications Specialist, Office of Public Affairs Where do I watch? To watch this livestream, go to energy.gov/live. Tune in to a special livestream at energy.gov/live on Friday, Sept. 14 at 10 a.m. ET for a science lecture about the Higgs boson with Joe Incandela, head spokesperson for the CMS experiment at CERN's Large Hadron Collider. Hosted by Secretary of Energy Steven Chu, tune in to the livestream to hear about the research conducted by CERN and the Energy Department. Addthis Related Articles WATCH LIVE: Talking the Higgs Boson with CERN

373

DOE Solar Decathlon: Cornell University: Living the Good (Solar) Life  

NLE Websites -- All DOE Office Websites (Extended Search)

Cornell University's Solar Decathlon 2007 house viewed from the southeast in its permanent location near New York's Finger Lakes. Cornell University's Solar Decathlon 2007 house viewed from the southeast in its permanent location near New York's Finger Lakes. Enlarge image Light Canopy's owners made additions to the house that honored the decathletes' original vision. (Courtesy of Mike Koplinka-Loehr) Who: Cornell University What: Light Canopy Where: Lansing, NY 14882 Map This House Public tours: Open for the Northeast Sustainable Energy Association's Green Buildings Open House Tours each fall Solar Decathlon 2007 Cornell University: Living the Good (Solar) Life The Cornell University team auctioned off its solar-powered house, Light Canopy, to a private buyer after competing in the U.S. Department of Energy Solar Decathlon 2007. The Koplinka-Loehr family now resides in the house at its permanent location on the southeastern shore of New York's Cayuga Lake.

374

Jefferson Lab Science Series - Living and Working in the Freezer  

NLE Websites -- All DOE Office Websites (Extended Search)

Volcanoes in Virginia! Volcanoes in Virginia! Previous Video (Volcanoes in Virginia!) Science Series Video Archive Next Video (You Already Know This Physics!) You Already Know This Physics! Living and Working in the Freezer Dr. Victoria Hill - Old Dominion University, Bio-Optics Group February 7, 2012 Very little data of any kind exists from the early spring in the Arctic. The reason? It's extremely cold and that makes it difficult to survive, let alone conduct science. From March through the end of April, 2011, scientists from around the world braved temperatures of -48°C in the high Canadian Arctic in the name of science. At the Catlin Arctic Survey's floating 'Ice Base' off Ellef Ringnes Island, Dr. Victoria Hill was investigating how organic material in fresh water near the surface of the

375

A Catalog and Atlas of Cataclysmic Variables - The Living Edition  

E-Print Network (OSTI)

The Catalog and Atlas of Cataclysmic Variables (Edition 1 - 1993) and Edition 2 - 1997) has been a valuable source of information for the cataclysmic variable (CV) community. However, the goal of having a central location for all objects is slowly being lost as each new edition is generated. There can also be a long time delay between new information becoming available on an object and its publication in the catalog. To eliminate these concerns, as well as to make the catalog more accessible, we have created a web site which will contain a ``living'' edition of the catalog. We have also added orbital period information, as well as finding charts for novae, to the catalog.

Downes, R A; Shara, M M; Ritter, H G; Kolb, U; Duerbeck, H W; Ritter, Hans; Kolb, Ulrich; Duerbeck, Hilmar W.

2001-01-01T23:59:59.000Z

376

Reasoning Support for Risk Prediction and Prevention in Independent Living  

E-Print Network (OSTI)

In recent years there has been growing interest in solutions for the delivery of clinical care for the elderly, due to the large increase in aging population. Monitoring a patient in his home environment is necessary to ensure continuity of care in home settings, but, to be useful, this activity must not be too invasive for patients and a burden for caregivers. We prototyped a system called SINDI (Secure and INDependent lIving), focused on i) collecting a limited amount of data about the person and the environment through Wireless Sensor Networks (WSN), and ii) inferring from these data enough information to support caregivers in understanding patients' well being and in predicting possible evolutions of their health. Our hierarchical logic-based model of health combines data from different sources, sensor data, tests results, common-sense knowledge and patient's clinical profile at the lower level, and correlation rules between health conditions across upper levels. The logical formalization and the reasonin...

Mileo, A; Bisiani, R

2010-01-01T23:59:59.000Z

377

Living and Working Safely Around High-Voltage Power Lines.  

Science Conference Proceedings (OSTI)

High-voltage transmission lines can be just as safe as the electrical wiring in the homes--or just as dangerous. The crucial factor is ourselves: they must learn to behave safely around them. This booklet is a basic safety guide for those who live and work around power lines. It deals primarily with nuisance shocks due to induced voltages, and with potential electric shock hazards from contact with high-voltage lines. References on possible long-term biological effects of transmission lines are shown. In preparing this booklet, the Bonneville Power Administration has drawn on more than 50 years of experience with high-voltage transmission. BPA operates one of the world`s largest networks of long-distance, high-voltage lines. This system has more than 400 substations and about 15,000 miles of transmission lines, almost 4,400 miles of which are operated at 500,000 volts.

United States. Bonneville Power Administration.

2001-06-01T23:59:59.000Z

378

Compressive force generation by a bundle of living biofilaments  

E-Print Network (OSTI)

To study the compressional forces exerted by a bundle of living stiff filaments pressing on a surface, akin to the case of an actin bundle in filopodia structures, we have performed particulate Molecular Dynamics simulations of a grafted bundle of parallel living (self-assembling) filaments, in chemical equilibrium with a solution of their constitutive monomers. Equilibrium is established as these filaments, grafted at one end to a wall of the simulation box, grow at their chemically active free end and encounter the opposite confining wall of the simulation box. Further growth of filaments requires bending and thus energy, which automatically limit the populations of longer filaments. The resulting filament sizes distribution and the force exerted by the bundle on the obstacle are analyzed for different grafting densities and different sub- or supercritical conditions, these properties being compared with the predictions of the corresponding ideal confined bundle model. In this analysis, non-ideal effects due to interactions between filaments and confinement effects are singled out. For all state points considered at the same temperature and at the same gap width between the two surfaces, the force per filament exerted on the opposite wall appears to be a function of a rescaled free monomer density $\\hat{\\rho}_1^{\\rm eff}$. This quantity can be estimated directly from the characteristic length of the exponential filament size distribution $P$ observed in the size domain where these grafted filaments are not in direct contact with the wall. We also analyze the dynamics of the filament contour length fluctuations in terms of effective polymerization ($U$) and depolymerization ($W$) rates, where again it is possible to disentangle non-ideal and confinement effects.

Sanoop Ramachandran; Jean-Paul Ryckaert

2013-10-06T23:59:59.000Z

379

MOLECULAR APPROACHES FOR IN SITU IDENTIFCIATION OF NITRATE UTILIZATION BY MARINE BACTERIA AND PHYTOPLANKTON  

SciTech Connect

Traditionally, the importance of inorganic nitrogen (N) for the nutrition and growth of marine phytoplankton has been recognized, while inorganic N utilization by bacteria has received less attention. Likewise, organic N has been thought to be important for heterotrophic organisms but not for phytoplankton. However, accumulating evidence suggests that bacteria compete with phytoplankton for nitrate (NO3-) and other N species. The consequences of this competition may have a profound effect on the flux of N, and therefore carbon (C), in ocean margins. Because it has been difficult to differentiate between N uptake by heterotrophic bacterioplankton versus autotrophic phytoplankton, the processes that control N utilization, and the consequences of these competitive interactions, have traditionally been difficult to study. Significant bacterial utilization of DIN may have a profound effect on the flux of N and C in the water column because sinks for dissolved N that do not incorporate inorganic C represent mechanisms that reduce the atmospheric CO2 drawdown via the ?biological pump? and limit the flux of POC from the euphotic zone. This project was active over the period of 1998-2007 with support from the DOE Biotechnology Investigations ? Ocean Margins Program (BI-OMP). Over this period we developed a tool kit of molecular methods (PCR, RT-PCR, Q-PCR, QRT-PCR, and TRFLP) and combined isotope mass spectrometry and flow-cytometric approaches that allow selective isolation, characterization, and study of the diversity and genetic expression (mRNA) of the structural gene responsible for the assimilation of NO3- by heterotrophic bacteria (nasA). As a result of these studies we discovered that bacteria capable of assimilating NO3- are ubiquitous in marine waters, that the nasA gene is expressed in these environments, that heterotrophic bacteria can account for a significant fraction of total DIN uptake in different ocean margin systems, that the expression of nasA is differentially regulated in genetically distinct NO3- assimilating bacteria, and that the best predictors of nasA gene expression are either NO3- concentration or NO3- uptake rates. These studies provide convincing evidence of the importance of bacterial utilization of NO3-, insight into controlling processes, and provide a rich dataset that are being used to develop linked C and N modeling components necessary to evaluate the significance of bacterial DIN utilization to global C cycling. Furthermore, as a result of BI-OMP funding we made exciting strides towards institutionalizing a research and education based collaboration between the Skidaway Institute of Oceanography (SkIO) and Savannah State University (SSU), an historically black university within the University System of Georgia with undergraduate and now graduate programs in marine science. The BI-OMP program, in addition to supporting undergraduate (24) graduate (10) and postdoctoral (2) students, contributed to the development of a new graduate program in Marine Sciences at SSU that remains an important legacy of this project. The long-term goals of these collaborations are to increase the capacity for marine biotechnology research and to increase representation of minorities in marine, environmental and biotechnological sciences.

Frischer, Marc E. [Skidaway Institute of Oceanography; Verity, Peter G.; Gilligan, Mathew R.; Bronk, Deborah A.; Zehr, Jonathan P.; Booth, Melissa G.

2013-09-12T23:59:59.000Z

380

Laser Ablation of Organic Materials for discrimination of bacteria in an  

NLE Websites -- All DOE Office Websites (Extended Search)

Laser Ablation of Organic Materials for discrimination of bacteria in an Laser Ablation of Organic Materials for discrimination of bacteria in an organic background Title Laser Ablation of Organic Materials for discrimination of bacteria in an organic background Publication Type Conference Proceedings Year of Publication 2009 Authors Baudelet, Matthieu, Myriam Boueri, Jin Yu, Xianglei Mao, Samuel S. Mao, and Richard E. Russo Conference Name Ultrafast Phenomena in Semiconductors and Nanostructure Materials XIII Series Title Proceedings SPIE Volume 7214 Pagination 72140J Date Published 02/2009 Abstract We demonstrate in this paper that laser ablation allows efficient analysis of organic and biological materials. Such analysis is based on laser-induced breakdown spectroscopy (LIBS) which consists in the detection of the optical emission from the plasma induced by a high intensity laser pulse focused on the sample surface. The optimization of the ablation regime in terms of laser parameters (pulse duration, wavelength, fluence) is important to generate a plasma suitable for the analysis. We first present the results of a study of laser ablation of organic samples with different laser parameters using time-resolved shadowgraph. We correlate the early stage expansion of the plasma to its optical emission properties, which allows us to choose suitable laser parameters for an efficient analysis of organic or biological samples by LIBS. As an illustration of the analytical ability of LIBS for biological materials, we show that the emission from CN molecules can be used to distinguish between biological and inorganic samples. Native CN molecular fragment directly ablated from a biological sample are identified using time-resolved LIBS. Those due to recombination with nitrogen contained in atmospheric air can be distinguished with their specific time evolution behavior.

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


381

Exploration of Simple Analytical Approaches for Rapid Detection of Pathogenic Bacteria  

Science Conference Proceedings (OSTI)

Many of the current methods for pathogenic bacterial detection require long sample-preparation and analysis time, as well as complex instrumentation. This dissertation explores simple analytical approaches (e.g., flow cytometry and diffuse reflectance spectroscopy) that may be applied towards ideal requirements of a microbial detection system, through method and instrumentation development, and by the creation and characterization of immunosensing platforms. This dissertation is organized into six sections. In the general Introduction section a literature review on several of the key aspects of this work is presented. First, different approaches for detection of pathogenic bacteria will be reviewed, with a comparison of the relative strengths and weaknesses of each approach, A general overview regarding diffuse reflectance spectroscopy is then presented. Next, the structure and function of self-assembled monolayers (SAMs) formed from organosulfur molecules at gold and micrometer and sub-micrometer patterning of biomolecules using SAMs will be discussed. This section is followed by four research chapters, presented as separate manuscripts. Chapter 1 describes the efforts and challenges towards the creation of imunosensing platforms that exploit the flexibility and structural stability of SAMs of thiols at gold. 1H, 1H, 2H, 2H-perfluorodecyl-1-thiol SAM (PFDT) and dithio-bis(succinimidyl propionate)-(DSP)-derived SAMs were used to construct the platform. Chapter 2 describes the characterization of the PFDT- and DSP-derived SAMs, and the architectures formed when it is coupled to antibodies as well as target bacteria. These studies used infrared reflection spectroscopy (IRS), X-ray photoelectron spectroscopy (XPS), and electrochemical quartz crystal microbalance (EQCM), Chapter 3 presents a new sensitive, and portable diffuse reflection based technique for the rapid identification and quantification of pathogenic bacteria. Chapter 4 reports research efforts in the construction and evaluation of a prototype flow cytometry based cell detector and enumerator. This final research chapter is followed by a general summation and future prospectus section that concludes this dissertation.

Salma Rahman

2005-12-17T23:59:59.000Z

382

Light-harvesting in bacteria exploits a critical interplay between transport and trapping dynamics  

E-Print Network (OSTI)

Light-harvesting bacteria Rhodospirillum Photometricum were recently found to adopt strikingly different architectures depending on illumination conditions. We present analytic and numerical calculations which explain this observation by quantifying a dynamical interplay between excitation transfer kinetics and reaction center cycling. High light-intensity membranes (HLIM) exploit dissipation as a photo-protective mechanism, thereby safeguarding a steady supply of chemical energy, while low light-intensity membranes (LLIM) efficiently process unused illumination intensity by channelling it to open reaction centers. More generally, our analysis elucidates and quantifies the trade-offs in natural network design for solar energy conversion.

Felipe Caycedo-Soler; Ferney J. Rodriguez; Luis Quiroga; Neil F. Johnson

2010-03-11T23:59:59.000Z

383

GRR/Section 12-WA-a - Live Wildlife Taking Permit | Open Energy Information  

Open Energy Info (EERE)

GRR/Section 12-WA-a - Live Wildlife Taking Permit GRR/Section 12-WA-a - Live Wildlife Taking Permit < GRR Jump to: navigation, search GRR-logo.png GEOTHERMAL REGULATORY ROADMAP Roadmap Home Roadmap Help List of Sections Section 12-WA-a - Live Wildlife Taking Permit 12-WA-a - Live Wildlife Taking Permit.pdf Click to View Fullscreen Contact Agencies Washington State Department of Fish and Wildlife Regulations & Policies WAC 232-12-064 Triggers None specified In Washington, it is unlawful to take wildlife from the wild without permission from the Washington State Department of Fish and Wildlife (WDFW). The WDFW issues Live Wildlife Taking Permits under WAC 232-12-064. 12-WA-a - Live Wildlife Taking Permit.pdf Error creating thumbnail: Page number not in range. Error creating thumbnail: Page number not in range.

384

TODAY MAY 22ND: Live Tweeting the Apps for Energy Winners Announcement! |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

TODAY MAY 22ND: Live Tweeting the Apps for Energy Winners TODAY MAY 22ND: Live Tweeting the Apps for Energy Winners Announcement! TODAY MAY 22ND: Live Tweeting the Apps for Energy Winners Announcement! May 22, 2012 - 10:09am Addthis TODAY MAY 22ND: Live Tweeting the Apps for Energy Winners Announcement! Erin R. Pierce Erin R. Pierce Digital Communications Specialist, Office of Public Affairs How can I participate? Join us as we live tweet the Apps for Energy winners announcement event. To participate, simply follow #appsforenergy on Twitter, beginning at 11:30 am EDT. Join us today, May 22 at 11:30 am EDT as we live tweet (@ENERGY) the Apps for Energy winners announcement event at ConnectivityWeek 2012 in Santa Clara, California. To get real-time updates on the winning applications as they are announced, follow #appsforenergy on twitter.

385

Follow Live Dec 24: Los Alamos National Lab Tracks Rudolph's Nose,  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Follow Live Dec 24: Los Alamos National Lab Tracks Rudolph's Follow Live Dec 24: Los Alamos National Lab Tracks Rudolph's Nose, Santa's Sleigh Follow Live Dec 24: Los Alamos National Lab Tracks Rudolph's Nose, Santa's Sleigh December 23, 2011 - 8:40am Addthis Thanks to the Los Alamos National Laboratory, you can follow along live as Santa circles the globe this Christmas Eve. | Image credit: Hantz Leger. Thanks to the Los Alamos National Laboratory, you can follow along live as Santa circles the globe this Christmas Eve. | Image credit: Hantz Leger. Ginny Simmons Ginny Simmons Former Managing Editor for Energy.gov, Office of Public Affairs What are the key facts? You can follow along live as Santa circles the globe this Christmas Eve. Santa enjoys a wide variety of cookies.

386

U-194: Symantec LiveUpdate Administrator Lets Local Users Gain Elevated  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

4: Symantec LiveUpdate Administrator Lets Local Users Gain 4: Symantec LiveUpdate Administrator Lets Local Users Gain Elevated Privledges U-194: Symantec LiveUpdate Administrator Lets Local Users Gain Elevated Privledges June 19, 2012 - 7:00am Addthis PROBLEM: Symantec LiveUpdate Administrator Lets Local Users Gain Elevated Privileges . PLATFORM: Version(s): 2.3 and prior versions Abstract: Users Gain Elevated Privileges reference LINKS: Vendor Advisory SecurityTracker Alert ID: 1027182 CVE-2012-0304 IMPACT ASSESSMENT: Medium Discussion: A vulnerability was reported in Symantec LiveUpdate Administrator. A local user can obtain elevated privileges on the target system.The default installation of Symantec LiveUpdate Administrator installs files with full control privileges granted to the 'Everyone' group.A local user can exploit

387

Can I choose the electricity supplier where I live? - FAQ - U ...  

U.S. Energy Information Administration (EIA)

Can I choose the electricity supplier where I live? Some electric utility customers have the option to choose an alternate supplier of electricity.

388

Resiliency factors and substance use among Manitoba First Nation girls living on reserve.  

E-Print Network (OSTI)

??The purpose of this study was to examine the relationships between adversity, resiliency and substance use among Manitoba First Nation girls living on reserve, ages (more)

Campbell, Rhonda Dawn

2010-01-01T23:59:59.000Z

389

Forrestal Building Lighting Retrofit Second Live Test Demonstration (LTD)  

SciTech Connect

This report describes and summarizes the Forrestal Building Lighting Retrofit Live Test demonstration (LTD) performed by Pacific Northwest Laboratory (PNL) in Room 5E-080 of the DOE Forrestal Building in Washington, D.C. The purpose of the LTD was to evaluate proposed lighting retrofits for compliance with the requirements laid out in the request for proposal (RFP) for the Shared Energy Savings (SES) Lighting Retrofit Project for the Forrestal Building, Washington, D.C. Testing was conducted from March 9 through March 18, 1992, and again on August 3 through August 6, 1992. Four contractors were initially tested in March. Then, two contractors were retested in August due to changes in the rebate schedule for electronic ballasts being offered by the Potomac Electric Power Company (PEPCO), the utility servicing the Forrestal Building. The two contractors tested in March were retested with different ballasts, tubes, and reflectors. The results from these new tests are reported here and compared with those from the earlier tests.

Halverson, M.A.; Schmelzer, J.R.; Parker, G.B.

1993-02-01T23:59:59.000Z

390

Solar Decathlon 2002: Energy We Can Live With (Program Brochure)  

DOE Green Energy (OSTI)

This program brochure will be handed out to the teams, sponsors, and some attendees to provide a brief overview of the competition and the fourteen entries. The brochure also outlines the sponsors reasons for participating in the Solar Decathlon. The U.S. Department of Energy is proud to sponsor the first-ever Solar Decathlon, a college and university competition that brings together our nation's brightest minds to demonstrate practical ways of producing and using energy efficiently in the home. The Solar Decathlon consists of 10 contests that encompass all the ways in which we use energy in our daily lives--from livability and comfort to daily chores and home-based work to getting around town. Sunlight is the only source of energy that can be used to generate the thermal, electrical, and mechanical power needed to compete in the 10 contests. The best looking house that can produce the most energy and use that energy the most efficiently will win. Energy efficiency and solar technologies are available for the home today, and they are affordable. At the same time, the designs of these homes are attractive and livable. The Solar Decathlon will prove that investment in renewable energy and energy-efficient technologies can reduce our dependence on foreign oil, improve human health, conserve natural resources, and create markets for American products around the world.

Not Available

2002-09-01T23:59:59.000Z

391

Structural similarities between biogenic uraninites produced by phylogenetically and metabolically diverse bacteria.  

SciTech Connect

While the product of microbial uranium reduction is often reported to beUO2, a comprehensive characterization including stoichiometry and unit cell determination is available for only one Shewanella species. Here, we compare the products of batch uranyl reduction by a collection of dissimilatory metal- and sulfate-reducing bacteria of the genera Shewanella, Geobacter, Anaeromyxobacter, and Desulfovibrio under similar laboratory conditions. Our results demonstrate that U(VI) bioreduction by this assortment of commonly studied, environmentally relevant bacteria leads to the precipitation of uraninite with a composition between UO2.00 and UO2.075, regardless of phylogenetic or metabolic diversity. Coupled analyses, including electron microscopy, X-ray absorption spectroscopy, and powder diffraction, confirm that structurally and chemically analogous uraninite solids are produced. These biogenic uraninites have particle diameters of about 2-3 nm and lattice constants consistent with UO2.0 and exhibit a high degree of intermediate-range order. Results indicate that phylogenetic and metabolic variability within delta- and gamma-proteobacteria has little effect on nascent biouraninite structure or crystal size under the investigated conditions.

Sharp, Jonathan; Schofield, Eleanor J.; Veeramani, Harish; Suvorova, Elena; Kennedy, David W.; Marshall, Matthew J.; Mehta, Apurva; Bargar, John R.; Bernier-Latmani, Rizlan

2009-11-01T23:59:59.000Z

392

Structure, Function and Reconstitution of Antenna Complexes of Green Photosynthetic Bacteria  

DOE Green Energy (OSTI)

Most chlorophyll-type pigments in a photosynthetic organism function as an antenna, absorbing light and transferring excitations to a photochemical reaction center where energy storage takes place by a series of chemical reactions. The green photosynthetic bacteria are characterized by large antenna complexes known as chlorosomes, in which pigment-pigment interactions are of dominant importance. The overall objective of this project is to determine the mechanisms of excitation transfer and regulation of this unique antenna system, including how it is integrated into the rest of the photosynthetic energy transduction apparatus. Techniques that are being used in this research include biochemical analysis, spectroscopy, microscopy, X-ray structural studies, and reconstitution from purified components. Our recent results indicate that the chlorosome baseplate structure, which is the membrane attachment site for the chlorosome to the membrane, is a unique pigment-protein that contains large amounts of carotenoids and small amounts of bacteriochlorophyll a. Reconstitution of directed energy transfer in chlorosomes will be carried out using purified baseplates and oligomeric pigments. The integral membrane B808-866 antenna complex from Chloroflexus aurantiacus and the Fenna-Matthews-Olson protein-reaction center complex from green sulfur bacteria will be characterized by spectroscopic and structural techniques.

Blankenship, Robert E.

2005-06-10T23:59:59.000Z

393

Bioinspired Materials Engineering - Programmaster.org  

Science Conference Proceedings (OSTI)

... materials synthesis mediated by microorganisms (bacteria, virus, fungi, algae), biopolymer/ceramic composites. Microstructure pattern formation for functional...

394

Environmental Microbiology  

NLE Websites -- All DOE Office Websites (Extended Search)

Environmental Microbiology Environmental Microbiology Environmental Microbiology Los Alamos working to identify genetic regulatory systems in single microorganisms. Get Expertise Cheryl Kuske DOE BER Biological System Science Division Program Manager Email Srinivas Iyer Bioscience Group Leader Email Rebecca McDonald Bioscience Communications Email Examining the soil beneath our feet Environmental microbiology Read caption + Many environmental molecular biology studies begin with purified DNA and RNA extracted from the soil. Overview of Research and Highlights Learning about microorganisms-bacteria, algae, and fungi-is essential to understanding how living things interact with their environments. Exploration of environmental microbiology at Los Alamos crosses broad scales of investigation that span from identification of genetic regulatory

395

Environmental Microbiology  

NLE Websites -- All DOE Office Websites (Extended Search)

Environmental Microbiology Environmental Microbiology Examining the soil beneath our feet Read caption + Many environmental molecular biology studies begin with purified DNA and RNA extracted from the soil. Overview of Research and Highlights Learning about microorganisms-bacteria, algae, and fungi-is essential to understanding how living things interact with their environments. Exploration of environmental microbiology at Los Alamos crosses broad scales of investigation that span from identification of genetic regulatory systems in single microorganisms to comprehensive studies of the complex microbial communities resident in soil, water and air. The long term goals of this research are to understand microbial processes and interactions, and the genomic traits underlying these activities, toward:

396

The hydrodynamics of swimming microorganisms  

E-Print Network (OSTI)

Cell motility in viscous fluids is ubiquitous and affects many biological processes, including reproduction, infection, and the marine life ecosystem. Here we review the biophysical and mechanical principles of locomotion at the small scales relevant to cell swimming (tens of microns and below). The focus is on the fundamental flow physics phenomena occurring in this inertia-less realm, and the emphasis is on the simple physical picture. We review the basic properties of flows at low Reynolds number, paying special attention to aspects most relevant for swimming, such as resistance matrices for solid bodies, flow singularities, and kinematic requirements for net translation. Then we review classical theoretical work on cell motility: early calculations of the speed of a swimmer with prescribed stroke, and the application of resistive-force theory and slender-body theory to flagellar locomotion. After reviewing the physical means by which flagella are actuated, we outline areas of active research, including hydrodynamic interactions, biological locomotion in complex fluids, the design of small-scale artificial swimmers, and the optimization of locomotion strategies.

Eric Lauga; Thomas R. Powers

2008-12-15T23:59:59.000Z

397

Roles of Naturally Occurring Bacteria in Controlling Iodine-129 Mobility in Subsurface Soils  

E-Print Network (OSTI)

129I is of major concern because of its biophilic nature, excessive inventory, long half-life (~16 million yrs), and high mobility in the natural environment that depends on its chemical speciation. Iodide (I-) has the highest mobility than iodate (IO3-) and is the predominant species in the terrestrial environment due to prevailing pH and Eh conditions. In order to transform I- to less mobile organo-iodine (OI), strong oxidants are necessary to activate the first electron transfer step from I- to reactive intermediates. The aim of this study was to determine the influence of naturally occurring aerobic bacteria isolated from an 129I contaminated aquifer (F-area of the Savannah River Site, SC) on I- oxidation and OI formation. It was demonstrated that 3 of 136 strains accumulated I- (0.2~2%) in the presence of H2O2, when incubated in the presence of an environmentally relevant concentration of I- (0.1 microM). The accumulation was likely through electrophilic substitution resulting in the iodination of cellular constituents. The results indicated that culturable I--accumulating bacteria are not directly responsible for the high fraction of oxidized iodine species (IO3- and OI, >50% of total I) present in the SRS F-area. Several bacterial strains were found to be capable of stimulating I- oxidation through excretion of oxidants and enzymes. Organic acids in spent liquid medium from 27 of 84 aerobic bacterial cultures enhanced H2O2-dependent I- oxidation 2-10 fold. Organic acids enhanced I- oxidation by (1) lowering the pH of the spent medium and (2) reacting with H2O2 to form peroxy carboxylic acids, which are strong oxidizing agents. In the absence of H2O2, spent medium from 44 of 84 bacteria cultures showed I- oxidizing capacities. One I- oxidizing bacterium was studied to characterize its extracellular I- oxidizing component(s). The I- oxidizing capability from the spent medium was inactive by treatments with heat and H2O2 and absent under anaerobic conditions. Conversely, NADH, NADPH and FMN additions stimulated I- oxidation in the spend medium. These results indicate an oxidase(s) catalyzed I- oxidation. Understanding the bacterial activities involved with I- oxidation and OI formation is expected to help reduce 129I mobility in water-soil systems.

Li, Hsiu-Ping

2012-08-01T23:59:59.000Z

398

An Observational Examination of Long-Lived Supercells. Part II: Environmental Conditions and Forecasting  

Science Conference Proceedings (OSTI)

The local and larger-scale environments of 184 long-lived supercell events (containing one or more supercells with lifetimes ?4 h; see Part I of this paper) are investigated and subsequently compared with those from 137 moderate-lived events (...

Matthew J. Bunkers; Jeffrey S. Johnson; Lee J. Czepyha; Jason M. Grzywacz; Brian A. Klimowski; Mark R. Hjelmfelt

2006-10-01T23:59:59.000Z

399

Ambient Assisted Living and Care in The Netherlands: The Voice of the User  

Science Conference Proceedings (OSTI)

Technology can assist older adults to remain living in the community. Within the realm of information and communication technologies, smart homes are drifting toward the concept of ambient assisted living (AAL). AAL-systems are more responsive to user ... Keywords: Ambient Intelligence, Assistive Technology, Needs, Older Adults, Smart Homes, User Perspectives

J. van Hoof; E. J. M. Wouters; H. R. Marston; B. Vanrumste; R. A. Overdiep

2011-10-01T23:59:59.000Z

400

Fabrication of a microfluidic platform for investigating dynamic biochemical processes in living samples by FTIR microspectroscopy  

Science Conference Proceedings (OSTI)

Here we present the optimization of fabrication steps for realizing an infrared-visible microfluidic chip to study single-living cell behaviour in physiological environment by synchrotron radiation FTIR microspectroscopy. We optimized subtractive and ... Keywords: FTIR microspectroscopy, Living-cells, Microfabrication, Microfluidic

Giovanni Birarda; Gianluca Grenci; Luca Businaro; Benedetta Marmiroli; Sabrina Pacor; Lisa Vaccari

2010-05-01T23:59:59.000Z

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


401

Optimization of microfluidic systems for IRMS long term measurement of living cells  

Science Conference Proceedings (OSTI)

Infrared Microspectroscopy (IRMS) has been proposed as a powerful diagnostic tool in biology, due to the rich molecular, structural and conformational information contained in IR spectra of cells and tissues. In particular, IRMS of live cells in microfluidic ... Keywords: -FTIR, Living cells, Microfluidic, Synchrotron radiation

G. Grenci; G. Birarda; E. Mitri; L. Businaro; S. Pacor; L. Vaccari; M. Tormen

2012-10-01T23:59:59.000Z

402

Our Energy Independence - A Live Chat With Dr. Arun Majumdar | Department  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Our Energy Independence - A Live Chat With Dr. Arun Majumdar Our Energy Independence - A Live Chat With Dr. Arun Majumdar Our Energy Independence - A Live Chat With Dr. Arun Majumdar June 29, 2011 - 11:16am Addthis Dr. Arun Majumdar takes your questions about investments we're making to build the clean energy infrastructure of the future. Ginny Simmons Ginny Simmons Former Managing Editor for Energy.gov, Office of Public Affairs How can I participate? Submit a question via E-mail, Facebook or Twitter Watch live at 2 PM [UPDATED with video from the event.] Before Americans hop into cars and planes to travel for this Independence Day weekend, we want to talk with you today about what we're doing to reduce our country's dependence on foreign oil. Please join Dr. Arun Majumdar at 2 PM ET for a live, two-way conversation about the

403

Ask the Directors: Secretary Chu to Host Live Chat on the Energy Innovation  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Ask the Directors: Secretary Chu to Host Live Chat on the Energy Ask the Directors: Secretary Chu to Host Live Chat on the Energy Innovation Hubs Ask the Directors: Secretary Chu to Host Live Chat on the Energy Innovation Hubs March 5, 2012 - 12:32pm Addthis Secretary Chu is accepting questions on Facebook, Twitter and e-mail for the Energy Innovation Hub directors -- who are working to build better nuclear reactors, obtain fuel from sunlight and design the most energy efficient buildings to date. Michael Hess Michael Hess Former Digital Communications Specialist, Office of Public Affairs How can I participate? Submit your questions on Facebook, Twitter (@energy), or send an e-mail to newmedia@hq.doe.gov, prior to or during the live event. Secretary Chu will host a live, streaming Q&A session with the directors of the Energy Innovation Hubs on Tuesday, March 6, at 2:15 p.m. EST at

404

Have You Visited the Living Zero Home Tour? | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Have You Visited the Living Zero Home Tour? Have You Visited the Living Zero Home Tour? Have You Visited the Living Zero Home Tour? August 20, 2009 - 12:00pm Addthis The Living Zero Home Tour is on the road, and it may be in a city near you! If you have the chance to see the tour, you may be surprised to discover the small steps that you can take to save energy and money. Have you visited the Living Zero Home Tour? Tell us about your visit in the comments. Each Thursday, you have the chance to share your thoughts on a question about energy efficiency or renewable energy for consumers. Please comment with your answers, and also feel free to respond to other comments. Addthis Related Articles Are You Going to the Solar Decathlon? How Do You Encourage Your Family to Use Less Water? How Do You Light Your Home Efficiently?

405

Green Living, Green Technologies: Things to Be Thankful For | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Green Living, Green Technologies: Things to Be Thankful For Green Living, Green Technologies: Things to Be Thankful For Green Living, Green Technologies: Things to Be Thankful For November 24, 2009 - 7:00am Addthis Drew Bittner Web Manager, Office of Energy Efficiency and Renewable Energy It's nearly Thanksgiving, the second of the "big" holidays that break up the last three months of the year. One of the most common family traditions is listing the things for which we are thankful. These are mostly items such as "family," "friends" or "good health." (I'm particularly thankful for the birth of my daughter back in February.) But let's think about something else for which we can be thankful. We live in a time where improvements in technology make it possible to live a happy, healthy, and productive life while using less and less energy.

406

Ask the Directors: Secretary Chu to Host Live Chat on the Energy Innovation  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Ask the Directors: Secretary Chu to Host Live Chat on the Energy Ask the Directors: Secretary Chu to Host Live Chat on the Energy Innovation Hubs Ask the Directors: Secretary Chu to Host Live Chat on the Energy Innovation Hubs March 5, 2012 - 12:32pm Addthis Secretary Chu is accepting questions on Facebook, Twitter and e-mail for the Energy Innovation Hub directors -- who are working to build better nuclear reactors, obtain fuel from sunlight and design the most energy efficient buildings to date. Michael Hess Michael Hess Former Digital Communications Specialist, Office of Public Affairs How can I participate? Submit your questions on Facebook, Twitter (@energy), or send an e-mail to newmedia@hq.doe.gov, prior to or during the live event. Secretary Chu will host a live, streaming Q&A session with the directors of the Energy Innovation Hubs on Tuesday, March 6, at 2:15 p.m. EST at

407

Our Energy Independence - A Live Chat With Dr. Arun Majumdar | Department  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Our Energy Independence - A Live Chat With Dr. Arun Majumdar Our Energy Independence - A Live Chat With Dr. Arun Majumdar Our Energy Independence - A Live Chat With Dr. Arun Majumdar June 29, 2011 - 11:16am Addthis Dr. Arun Majumdar takes your questions about investments we're making to build the clean energy infrastructure of the future. Ginny Simmons Ginny Simmons Former Managing Editor for Energy.gov, Office of Public Affairs How can I participate? Submit a question via E-mail, Facebook or Twitter Watch live at 2 PM [UPDATED with video from the event.] Before Americans hop into cars and planes to travel for this Independence Day weekend, we want to talk with you today about what we're doing to reduce our country's dependence on foreign oil. Please join Dr. Arun Majumdar at 2 PM ET for a live, two-way conversation about the

408

Antenna organization in green photosynthetic bacteria. Progress report, July 1, 1985--June 30, 1987  

DOE Green Energy (OSTI)

This project is concerned with the structure and function of the unique antenna system found in the green photosynthetic bacteria. The antenna system in these organisms is contained within a vesicle known as a chlorosome, which is attached to the cytoplasmic side of the cell membrane. Additional antenna pigments and reaction centers are contained in integral membrane proteins. Energy absorbed by the bacteriochlorophyll c (BChl c) pigments in the chlorosome is transferred via a ``baseplate`` array of BChl a antenna pigments into the membrane and to the reaction center. A schematic model of chlorosome structure is shown. This project is aimed at increasing our understanding of the organization of the pigments in the chlorosome and how the antenna system functions.

Blankenship, R.E.

1987-12-31T23:59:59.000Z

409

Excitonic energy transfer in light-harvesting complexes in purple bacteria  

Science Conference Proceedings (OSTI)

Two distinct approaches, the Frenkel-Dirac time-dependent variation and the Haken-Strobl model, are adopted to study energy transfer dynamics in single-ring and double-ring light-harvesting (LH) systems in purple bacteria. It is found that the inclusion of long-range dipolar interactions in the two methods results in significant increase in intra- or inter-ring exciton transfer efficiency. The dependence of exciton transfer efficiency on trapping positions on single rings of LH2 (B850) and LH1 is similar to that in toy models with nearest-neighbor coupling only. However, owing to the symmetry breaking caused by the dimerization of BChls and dipolar couplings, such dependence has been largely suppressed. In the studies of coupled-ring systems, both methods reveal an interesting role of dipolar interactions in increasing energy transfer efficiency by introducing multiple intra/inter-ring transfer paths. Importantly, the time scale (4 ps) of inter-ring exciton transfer obtained from polaron dynamics is in good agreement with previous studies. In a double-ring LH2 system, non-nearest neighbor interactions can induce symmetry breaking, which leads to global and local minima of the average trapping time in the presence of a non-zero dephasing rate, suggesting that environment dephasing helps preserve quantum coherent energy transfer when the perfect circular symmetry in the hypothetic system is broken. This study reveals that dipolar coupling between chromophores may play an important role in the high energy transfer efficiency in the LH systems of purple bacteria and many other natural photosynthetic systems.

Ye Jun; Sun Kewei; Zhao Yang; Lee, Chee Kong [School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798 (Singapore); Yu Yunjin [School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798 (Singapore); College of Physics Science and Technology, Shenzhen University, Guangdong 518060 (China); Cao Jianshu [Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States)

2012-06-28T23:59:59.000Z

410

Lipid Analysis and Lipidomics: New Techniques & ApplicationChapter 12 Fast GC for Cellular FAME Analysis of Bacteria  

Science Conference Proceedings (OSTI)

Lipid Analysis and Lipidomics: New Techniques & Application Chapter 12 Fast GC for Cellular FAME Analysis of Bacteria Methods and Analyses eChapters Methods - Analyses Books 1FEE4C7C73C70C0CBEFB8C79B2926801 AOCS Press

411

Study on the Isolation Screening and Characteristic Identification of Denitrifying Phosphorus Removing Bacteria in Sequencing Batch Biofilm Reactor  

Science Conference Proceedings (OSTI)

The processes of denitrification and dephosphorization which were independent originally were integrated into Biological Denitrifying Phosphorus and removing process. It was widely considered a bright technology of biological phosphorus removal. According ... Keywords: denitrifying phosphorus removing bacteria, enrichment and screening, characteristic identification, flat plate scribing method, metachromatic granules dyeing, nitrate reduction test

Yafeng Li; Hongtao Liu; Jing Ren

2012-05-01T23:59:59.000Z

412

Comparison of vetiver root essential oils from cleansed (bacteria-and fungus-free) vs. non-cleansed  

E-Print Network (OSTI)

tissue culture derived and natural plants for both genotypes. Although oil yields differed, this may cultivars used for commercial essential oil production are `Sunshine' or very similar cultivars. This workComparison of vetiver root essential oils from cleansed (bacteria- and fungus-free) vs. non

Adams, Robert P.

413

Novel Thermo-Acidophilic Bacteria Isolated from Geothermal Sites in Yellowstone National Park: Physiological and Phylogenetic Characteristics  

Science Conference Proceedings (OSTI)

Moderately thermophilic acidophilic bacteria were isolated from geothermal (3083 C) acidic (pH 2.7 3.7) sites in Yellowstone National Park. The temperature maxima and pH minima of the isolates ranged from 50 to 65 C, and pH 1.01.9. Eight of the bacteria were able to catalyze the dissimilatory oxidation of ferrous iron, and eleven could reduce ferric iron to ferrous iron in anaerobic cultures. Several of the isolates could also oxidize tetrathionate. Six of the iron-oxidizing isolates, and one obligate heterotroph, were low G+C gram-positive bacteria (Firmicutes). The former included three Sulfobacillus-like isolates (two closely related to a previously isolated Yellowstone strain, and the third to a mesophilic bacterium isolated from Montserrat), while the other three appeared to belong to a different genus. The other two iron-oxidizers were an Actinobacterium (related to Acidimicrobium ferrooxidans) and a Methylobacterium-like isolate (a genus within the a-Proteobacteria that has not previously been found to contain either iron-oxidizers or acidophiles). The other three (heterotrophic) isolates were also a-Proteobacteria and appeared be a novel thermophilic Acidisphaera sp. An ARDREA protocol was developed to discriminate between the iron-oxidizing isolates. Digestion of amplified rRNA genes with two restriction enzymes (SnaBI and BsaAI) separated these bacteria into five distinct groups; this result was confirmed by analysis of sequenced rRNA genes.

D. B. Johnson; N. Okibe; F. F. Roberto

2003-07-01T23:59:59.000Z

414

Bacteria that generate significant amounts of electricity could be used in microbial fuel cells to provide power in remote environments or to convert  

E-Print Network (OSTI)

Bacteria that generate significant amounts of electricity could be used in microbial fuel cells, "using this bacterial strain in a fuel cell to generate electricity would greatly increase the cell were more efficient at transferring electrons to generate power in fuel cells than bacteria

Lovley, Derek

415

Making more efficient fuel cells 08.09.2009 -Bacteria that generate significant amounts of electricity could be used in microbial fuel cells to provide  

E-Print Network (OSTI)

Making more efficient fuel cells 08.09.2009 - Bacteria that generate significant amounts of electricity could be used in microbial fuel cells to provide power in remote environments or to convert waste to generate electricity would greatly increase the cell's power output." The pili on the bacteria's surface

Lovley, Derek

416

Occurrence, Prevalence, and Disinfection Potential of Tetracycline Resistance Genes and Tetracycline Resistant Bacteria in a Subtropical Watershed  

E-Print Network (OSTI)

Antibiotics are an important method for protecting human health. Unfortunately, the development of antibiotic resistance has decreased the effectiveness of antibiotics in treating disease and preventing deaths associated with bacterial infection. The objective of this dissertation research was to gain a better understanding of anthropogenic influences on occurrence of tetracycline resistance and use of traditional disinfection methods for the reduction of tetracycline resistant bacteria and genes. Culture based and molecular methods were used to evaluate the occurrence of tetracycline resistance in a rapidly urbanizing watershed, identify the dominant resistant organisms and resistance genes in the watershed, and evaluate the use of UV and chlorine to reduce the concentration of resistant bacteria and resistance genes. Results from this research showed that tetracycline resistance was prevalent and is maintained in this study area. Several bacterial species (Aeromonas, Acinetobacter, Chryseobacterium, E. coli, Pseudomonas, and Serratia) made up the resistant population. The results also indicated that tet(W) was the major resistance gene in this watershed and that a majority of the resistant bacteria were capable of transferring their resistance. Landuse did not cause a difference in occurrence of resistant bacteria or resistance genes which suggests that a rapidly urbanizing watershed could experience resistance. It was also identified that environmental media (sediment and water) influence the occurrence and prevalence of resistant bacteria and resistance genes. The results indicate that streambed sediment may act as a reservoir for resistance and resistance might be transported in the water. Finally, the results showed that neither UV nor chlorine disinfection were effective in reducing tet(W) concentrations though the results varied greatly among species. Results from this research indicate that preventing the occurrence and distribution of resistance gene in the environment is difficult, and resistance will most likely be maintained. Therefore, in order to prevent the spread of antibiotic resistance, it will be important to prevent antibiotic resistance from becoming established in the environment. This can be done by educating the public about the importance of misusing and mismanaging antibiotics. Additionally, classifying antibiotics for either human or veterinary use may help slow the development of resistance. This should prevent clinically important antibiotics from being used in sub-therapeutic doses, which could decrease the selective pressure in the environment. Also clinically relevant bacteria can be prevented from interacting with resistant bacteria in the environment by disinfecting human waste.

Sullivan, Bailey Ann

2013-05-01T23:59:59.000Z

417

Summary Audit Report on Contractor Employee Relocation and Temporary Living Costs, IG-0400  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

DATE: January 27, 1997 DATE: January 27, 1997 REPLY TO ATTN OF: IG-1 SUBJECT: INFORMATION: Summary Audit Report on Contractor Employee Relocation andTemporary Living Costs TO: The Acting Secretary This summary report highlights systemic problems with contractor charges for contractor employee relocation and temporary living costs. Over the past 5 years, the Office of Inspector General issued nine audit reports that identified unreasonable and unallowable charges for employee relocation and temporary living costs by contractors and their subcontractors. We found that contractors were reimbursed for these costs because the Department of Energy (Department) did not use clearly defined contract provisions

418

Watch a Rare Earth Elements Event Live This Morning | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Watch a Rare Earth Elements Event Live This Morning Watch a Rare Earth Elements Event Live This Morning Watch a Rare Earth Elements Event Live This Morning December 15, 2010 - 9:20am Addthis Ginny Simmons Ginny Simmons Former Managing Editor for Energy.gov, Office of Public Affairs From 9:30am to noon ET today you can tune into a live discussion on "rare earth materials" that are critical to the production of clean energy technologies. Tune in here. The Department of Energy's Assistant Secretary for Policy and International Affairs David Sandalow will give the keynote, speaking to the role of rare earth metals and other materials in the clean energy economy. You can check back to the Energy Blog for more info later today. Ginny Simmons is a New Media Specialist and contractor to the Office of Public Affairs.

419

Join Us Live This Friday: Women in Clean Energy Symposium | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Us Live This Friday: Women in Clean Energy Symposium Us Live This Friday: Women in Clean Energy Symposium Join Us Live This Friday: Women in Clean Energy Symposium September 26, 2012 - 2:19pm Addthis NREL researcher Kirsten Alberi works in the luminescence mapping laboratory at the Solar Energy Research Facility (SERF). Here she aligns a laser and sample to map the photoluminescence coming off the sample. | Photo by Dennis Schroeder, NREL. NREL researcher Kirsten Alberi works in the luminescence mapping laboratory at the Solar Energy Research Facility (SERF). Here she aligns a laser and sample to map the photoluminescence coming off the sample. | Photo by Dennis Schroeder, NREL. Erin R. Pierce Erin R. Pierce Digital Communications Specialist, Office of Public Affairs How can I participate? Register for the live webcast, starting at 8:30 am ET on Friday,

420

Greensburg, Kansas--A Better, Greener Place to Live | Open Energy  

Open Energy Info (EERE)

Kansas--A Better, Greener Place to Live Kansas--A Better, Greener Place to Live Jump to: navigation, search Name Greensburg, Kansas--A Better, Greener Place to Live Agency/Company /Organization National Renewable Energy Laboratory Partner Greensburg GreenTown Focus Area Agriculture, Buildings, Commercial, Residential, Economic Development, Energy Efficiency, Industry, People and Policy, Renewable Energy, Geothermal, Water Power, Solar, - Solar Hot Water, - Solar Pv, Wind Phase Create a Vision, Develop Goals, Prepare a Plan, Develop Finance and Implement Projects, Create Early Successes Resource Type Case studies/examples Availability Publicly available--Free Publication Date 3/17/2011 Website http://apps1.eere.energy.gov/b Locality Greensburg, Kansas References Greensburg, Kansas--A Better, Greener Place to Live[1]

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


421

U-062: Pidgin SILC (Secure Internet Live Conferencing) Protocol Denial of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

2: Pidgin SILC (Secure Internet Live Conferencing) Protocol 2: Pidgin SILC (Secure Internet Live Conferencing) Protocol Denial of Service Vulnerability U-062: Pidgin SILC (Secure Internet Live Conferencing) Protocol Denial of Service Vulnerability December 15, 2011 - 8:00am Addthis PROBLEM: Pidgin SILC (Secure Internet Live Conferencing) Protocol Denial of Service Vulnerability. PLATFORM: Versions Prior to Pidgin 2.10.1 RedHat Enterprise Linux WS 4 RedHat Enterprise Linux Optional Productivity Application 5 server RedHat Enterprise Linux ES 4 RedHat Enterprise Linux Desktop Workstation 5 client RedHat Enterprise Linux Desktop version 4 Red Hat Enterprise Linux Desktop 5 client Red Hat Enterprise Linux AS 4 ABSTRACT: An attacker can exploit these issues by constructing and submitting a specially crafted SILC message. Successful exploits will cause the affected

422

One Thing Leads to Another: How NETL Research Saves Lives | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

One Thing Leads to Another: How NETL Research Saves Lives One Thing Leads to Another: How NETL Research Saves Lives One Thing Leads to Another: How NETL Research Saves Lives August 8, 2013 - 10:57am Addthis One Thing Leads to Another: How NETL Research Saves Lives Learn More The coronary stent was developed as part of NETL's Technology Transfer program. NETL's technology portfolio contains a broad range of innovations that have resulted from research in areas such as carbon capture and sequestration, mercury capture, fuel cells, sensors and controls, computational modeling, and materials science, among many others. NETL Technology Transfer program Balanced on the tip of a finger, it doesn't look like much - a bit of screen door, perhaps, or a badly mangled paper clip - but this little piece of metal is making big news in the medical community, and big changes

423

LiveChat Thurs, 10/20, 2pm ET: Clean Tech Markets | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

LiveChat Thurs, 10/20, 2pm ET: Clean Tech Markets LiveChat Thurs, 10/20, 2pm ET: Clean Tech Markets LiveChat Thurs, 10/20, 2pm ET: Clean Tech Markets October 17, 2011 - 11:30am Addthis Liisa O'Neill Liisa O'Neill Former New Media Specialist, Office of Public Affairs How can I participate? Send an email to newmedia@hq.doe.gov; Tweeting your question to @energy with the hashtag #energymatters; or leaving a question for Kauffman at Facebook.com/energygov. On Energy.gov, we've been showcasing a series of stories about innovations from our National Laboratories that have been successfully commercialized - and how they impact Americans' lives. During 2010 alone, our National Laboratories engaged in more than 13,500 technology transfer transactions - from licensing lab-developed technologies to using lab resources to drive industry innovation and commercial success.

424

Making the "Best Place to Live" Even Better | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Making the "Best Place to Live" Even Better Making the "Best Place to Live" Even Better Making the "Best Place to Live" Even Better August 4, 2010 - 11:24am Addthis Andy Oare Andy Oare Former New Media Strategist, Office of Public Affairs For 2010, the appropriately named Eden Prairie, Minnesota was honored as the No. 1 Best Place to Live in the United States by Money Magazine. The highly-coveted civic title reflects many aspects of the family-friendly suburb of 62,000 located 16 miles from downtown Minneapolis, including the advantages of being the home to major employers like Fortune 500 trucking company C.H. Robinson and hearing-aid maker Starkey Labs. The city also hass many natural amenities like 17 lakes and parks with 125 miles of running, hiking, and biking trails. One additional advantage that Eden Prairie will have going into next

425

University Teams to Showcase Affordable, Energy Efficient Living in U.S.  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

to Showcase Affordable, Energy Efficient Living in to Showcase Affordable, Energy Efficient Living in U.S. Department of Energy Solar Decathlon 2011 University Teams to Showcase Affordable, Energy Efficient Living in U.S. Department of Energy Solar Decathlon 2011 September 22, 2011 - 10:32am Addthis WASHINGTON, DC - Collegiate teams featuring over 4,000 students from around the world have descended on the National Mall's West Potomac Park to showcase the highly energy efficient solar-powered houses they created for the U.S. Department of Energy Solar Decathlon 2011. Today's opening ceremony kicks off the biennial competition that challenges collegiate teams to design, build, and operate houses powered by the sun that are affordable, energy efficient, attractive, and easy to live in. "The Solar Decathlon collegiate teams are showing how clean energy

426

Acting Biomass Program Manager Dr. Valerie Reed to Host Live Twitter Q&A on  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Acting Biomass Program Manager Dr. Valerie Reed to Host Live Acting Biomass Program Manager Dr. Valerie Reed to Host Live Twitter Q&A on Advanced Biofuels Acting Biomass Program Manager Dr. Valerie Reed to Host Live Twitter Q&A on Advanced Biofuels December 16, 2011 - 10:27am Addthis Washington, D.C. - On Friday, December 16th, the Energy Department (@energy) will be hosting a live Twitter Q&A on biofuels with Dr. Valerie Reed, Acting Manager of the Biomass Program. Dr. Reed holds a Ph. D. in Biochemistry from Georgetown University. In addition to her programmatic activities, Valerie is a founding member of the Metabolic Engineering Working Group, which is an interagency effort to advance metabolic engineering technologies for industrial, agricultural and human needs. She also co-chairs the Interagency Working Group on Conversion

427

Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Senior Adviser Richard Kauffman to Host Live Chat on Renewable Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and Deployment Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and Deployment October 19, 2011 - 3:00pm Addthis Washington, D.C. -- Thursday, October 20th, Richard Kauffman, Senior Adviser to the Secretary of Energy, will host the Department of Energy's third "Energy Matters" live chat. Kauffman will discuss the challenges and opportunities of renewable energy innovation and deployment. Kauffman recently joined the Energy Department from the private sector, where he served as the Chief Executive Officer of Good Energies, a global investor in renewable energy and energy efficiency technologies. Regarded as one of the country's leading experts on private sector investment in

428

TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's Oil Dependence Through Innovation TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's Oil Dependence Through Innovation June 28, 2011 - 12:00am Addthis WASHINGTON, D.C. - Today, Wednesday, June 29, ARPA-E Director Arun Majumdar will host the Department of Energy's second "Energy Matters" live chat. Dr. Majumdar will discuss the investments being made in innovative research and technology today that will move us off of foreign oil and toward the clean energy infrastructure of the future. You can submit your questions about new energy innovations to Dr. Majumdar in advance of the event through email, Twitter or Facebook, by Sending an email to newmedia@hq.doe.gov

429

LIVE Q&A TODAY: Answering Your Wind Energy Questions | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

LIVE Q&A TODAY: Answering Your Wind Energy Questions LIVE Q&A TODAY: Answering Your Wind Energy Questions LIVE Q&A TODAY: Answering Your Wind Energy Questions August 8, 2013 - 12:03pm Addthis On Thursday, August 8, we hosted a Google+ Hangout on wind energy in America. Watch as our clean energy experts take your questions. Erin R. Pierce Erin R. Pierce Digital Communications Specialist, Office of Public Affairs How can I participate? Tweet questions to @ENERGY with the hashtag #AskEnergy. Ask us on Facebook and Google+. Email questions to newmedia@hq.doe.gov. Watch the livestream on energy.gov/live or on the Google+ event page. Earlier this week, we released two new reports that showcase the record-breaking growth of the U.S. wind power market in 2012. Today, August 8, at 3 pm ET, we invite you to discuss key findings from the report by

430

Watch a Rare Earth Elements Event Live This Morning | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Watch a Rare Earth Elements Event Live This Morning Watch a Rare Earth Elements Event Live This Morning Watch a Rare Earth Elements Event Live This Morning December 15, 2010 - 9:20am Addthis Ginny Simmons Ginny Simmons Former Managing Editor for Energy.gov, Office of Public Affairs From 9:30am to noon ET today you can tune into a live discussion on "rare earth materials" that are critical to the production of clean energy technologies. Tune in here. The Department of Energy's Assistant Secretary for Policy and International Affairs David Sandalow will give the keynote, speaking to the role of rare earth metals and other materials in the clean energy economy. You can check back to the Energy Blog for more info later today. Ginny Simmons is a New Media Specialist and contractor to the Office of Public Affairs.

431

LIVE Q&A TODAY: Answering Your Wind Energy Questions | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

LIVE Q&A TODAY: Answering Your Wind Energy Questions LIVE Q&A TODAY: Answering Your Wind Energy Questions LIVE Q&A TODAY: Answering Your Wind Energy Questions August 8, 2013 - 12:03pm Addthis On Thursday, August 8, we hosted a Google+ Hangout on wind energy in America. Watch as our clean energy experts take your questions. Erin R. Pierce Erin R. Pierce Digital Communications Specialist, Office of Public Affairs How can I participate? Tweet questions to @ENERGY with the hashtag #AskEnergy. Ask us on Facebook and Google+. Email questions to newmedia@hq.doe.gov. Watch the livestream on energy.gov/live or on the Google+ event page. Earlier this week, we released two new reports that showcase the record-breaking growth of the U.S. wind power market in 2012. Today, August 8, at 3 pm ET, we invite you to discuss key findings from the report by

432

Efficient Living in a Small House: Could You Make the Switch? | Department  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Efficient Living in a Small House: Could You Make the Switch? Efficient Living in a Small House: Could You Make the Switch? Efficient Living in a Small House: Could You Make the Switch? November 10, 2009 - 7:00am Addthis Allison Casey Senior Communicator, NREL From time to time over the last few years, I've come across articles and blogs discussing the "Small House Movement." The movement encourages homeowners to reject the huge homes that dominate many suburban neighborhoods and embrace smaller homes and simpler living. Do a quick Web search on the movement and you'll find many photos and testimonials for these small homes-some smaller than 100 square feet. Many of these homes are fascinating. Not only are the designs innovative, attractive, and comfortable, they are also relatively inexpensive-especially when you consider long-term maintenance costs.

433

LIVE Q&A TODAY: Answering Your Wind Energy Questions | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

LIVE Q&A TODAY: Answering Your Wind Energy Questions LIVE Q&A TODAY: Answering Your Wind Energy Questions LIVE Q&A TODAY: Answering Your Wind Energy Questions August 8, 2013 - 12:03pm Addthis On Thursday, August 8, we hosted a Google+ Hangout on wind energy in America. Watch as our clean energy experts take your questions. Erin R. Pierce Erin R. Pierce Digital Communications Specialist, Office of Public Affairs How can I participate? Tweet questions to @ENERGY with the hashtag #AskEnergy. Ask us on Facebook and Google+. Email questions to newmedia@hq.doe.gov. Watch the livestream on energy.gov/live or on the Google+ event page. Earlier this week, we released two new reports that showcase the record-breaking growth of the U.S. wind power market in 2012. Today, August 8, at 3 pm ET, we invite you to discuss key findings from the report by

434

LiveChat Thurs, 10/20, 2pm ET: Clean Tech Markets | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

LiveChat Thurs, 10/20, 2pm ET: Clean Tech Markets LiveChat Thurs, 10/20, 2pm ET: Clean Tech Markets LiveChat Thurs, 10/20, 2pm ET: Clean Tech Markets October 17, 2011 - 11:30am Addthis Liisa O'Neill Liisa O'Neill Former New Media Specialist, Office of Public Affairs How can I participate? Send an email to newmedia@hq.doe.gov; Tweeting your question to @energy with the hashtag #energymatters; or leaving a question for Kauffman at Facebook.com/energygov. On Energy.gov, we've been showcasing a series of stories about innovations from our National Laboratories that have been successfully commercialized - and how they impact Americans' lives. During 2010 alone, our National Laboratories engaged in more than 13,500 technology transfer transactions - from licensing lab-developed technologies to using lab resources to drive industry innovation and commercial success.

435

Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Senior Adviser Richard Kauffman to Host Live Chat on Renewable Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and Deployment Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and Deployment October 19, 2011 - 3:00pm Addthis Washington, D.C. -- Thursday, October 20th, Richard Kauffman, Senior Adviser to the Secretary of Energy, will host the Department of Energy's third "Energy Matters" live chat. Kauffman will discuss the challenges and opportunities of renewable energy innovation and deployment. Kauffman recently joined the Energy Department from the private sector, where he served as the Chief Executive Officer of Good Energies, a global investor in renewable energy and energy efficiency technologies. Regarded as one of the country's leading experts on private sector investment in

436

TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

ARPA-E Director Arun Majumdar to Host Live Chat on Reducing ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's Oil Dependence Through Innovation TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's Oil Dependence Through Innovation June 28, 2011 - 12:00am Addthis WASHINGTON, D.C. - Today, Wednesday, June 29, ARPA-E Director Arun Majumdar will host the Department of Energy's second "Energy Matters" live chat. Dr. Majumdar will discuss the investments being made in innovative research and technology today that will move us off of foreign oil and toward the clean energy infrastructure of the future. You can submit your questions about new energy innovations to Dr. Majumdar in advance of the event through email, Twitter or Facebook, by Sending an email to newmedia@hq.doe.gov

437

In Situ Measurements of Long-Lived Trace Gases in the Lower Stratosphere by Gas Chromatography  

Science Conference Proceedings (OSTI)

Detailed information on the four-channel Airborne Chromatograph for Atmospheric Trace Species (ACATS-IV), used to measure long-lived atmospheric trace gases, is presented. Since ACATS-IV was last described in the literature, the temporal ...

P. A. Romashkin; D. F. Hurst; J. W. Elkins; G. S. Dutton; D. W. Fahey; R. E. Dunn; F. L. Moore; R. C. Myers; B. D. Hall

2001-07-01T23:59:59.000Z

438

Dr. Kathleen Hogan to Host Live Chat on Industrial Energy Efficiency  

Energy.gov (U.S. Department of Energy (DOE))

WASHINGTON, D.C. Kathleen Hogan, Assistant Deputy Secretary for Energy Efficiency, will host the Department of Energy's fourth "Energy Matters" live chat today. Dr. Hogan will discuss how the...

439

U.S. Bamboo house of the future : standardizing ecological living  

E-Print Network (OSTI)

This thesis focuses on ecological living through the use of bamboo. It explores how the material can be used for methods of prefabricated housing design within the United States. It also uses a "ht of parts" and describes ...

Wong, Lucy Lai

2006-01-01T23:59:59.000Z

440

M49-- Machine for the Living : a performance broadcast through an interfering FM radio transmission  

E-Print Network (OSTI)

The focus of the M49: Machine For The Living project is a performance work that considers the omnipresence of layered communications, which extend, yet supersede corporeal space of the individual. M49 creates a framework ...

Goldfarb, Maximilian M

2006-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "bacteria microorganisms living" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

Quantitative phase microscopy for the study of electromotility in living cells  

E-Print Network (OSTI)

The electric activity of living cells is accompanied with changes in their optical and mechanical properties, which arise from the intrinsic biophysics of the cell membrane. These intrinsic changes can be used as an indicator ...

Oh, Seung-eun

2010-01-01T23:59:59.000Z

442

Heat Watch/Warning Systems Save Lives: Estimated Costs and Benefits for Philadelphia 199598  

Science Conference Proceedings (OSTI)

The Philadelphia, Pennsylvania, Hot WeatherHealth Watch/Warning System was initiated in 1995 to alert the city's population to take precautionary actions when hot weather posed risks to health. The number of lives saved and the economic benefit ...

Kristie L. Ebi; Thomas J. Teisberg; Laurence S. Kalkstein; Lawrence Robinson; Rodney F. Weiher

2004-08-01T23:59:59.000Z

443

An Observational Examination of Long-Lived Supercells. Part I: Characteristics, Evolution, and Demise  

Science Conference Proceedings (OSTI)

Observations of supercells and their longevity across the central and eastern United States are examined, with the primary focus on understanding the properties of long-lived supercells (defined as supercells lasting ?4 h). A total of 224 long-...

Matthew J. Bunkers; Mark R. Hjelmfelt; Paul L. Smith

2006-10-01T23:59:59.000Z

444

Kinematic Vertical Motion and Relative Vorticity Profiles in a Long-Lived Midlatitude Convective System  

Science Conference Proceedings (OSTI)

Average kinematic vertical motion and relative vorticity profiles are presented for a long-lived midlatitude convective complex. A breakdown into active convective and stratiform precipitation regions shows very good agreement in the vertical ...

Lance F. Bosart

1986-06-01T23:59:59.000Z

445

Influence of Balanced Motions on Heavy Precipitation within a Long-Lived Convectively Generated Vortex  

Science Conference Proceedings (OSTI)

The forcing of heavy precipitation within a long-lived convectively generated mesoscale vortex (MCV) is investigated with the aid of diagnoses from Rapid Update Cycle gridded analyses. Organized convection within the MCV followed a distinct ...

Stanley B. Trier; Christopher A. Davis

2002-04-01T23:59:59.000Z

446

New reporters of protein trafficking and protein-protein interactions in live cells  

E-Print Network (OSTI)

Here, we describe our attempts to harness the exquisite specificity of natural protein and RNA enzymes to develop improved methods to study protein localization and protein-protein interactions in live cells. We first ...

Fernndez Surez, Marta

2008-01-01T23:59:59.000Z

447

Observed Enhancement of Reflectivity and the Electric Field in Long-Lived Florida Anvils  

Science Conference Proceedings (OSTI)

A study of two long-lived Florida anvils showed that reflectivity >20 dBZ increased in area, thickness, and sometimes magnitude at the midlevel well downstream of the convective cores. In these same regions electric fields maintained strengths >...

James E. Dye; John C. Willett

2007-10-01T23:59:59.000Z

448

Making lives under closure : birth and medicine in Palestine's waiting zones  

E-Print Network (OSTI)

Reproduction is a site for understanding the ways in which people reconceptualize and re-organize the world in which they live. This dissertation tries to understand the world of birth under the regime of closures and ...

Wick, Livia

2006-01-01T23:59:59.000Z

449

Green Campus, a Living Lab for the Smart Green Building Technology...  

NLE Websites -- All DOE Office Websites (Extended Search)

Green Campus, a Living Lab for the Smart Green Building Technology Integration Speaker(s): Hsu-Cheng Chiang Date: March 9, 2012 - 12:00pm Location: 90-3122 Seminar HostPoint of...

450

Inversion of long-lived trace gas emissions using combined Eulerian and Lagrangian chemical transport models  

E-Print Network (OSTI)

We present a method for estimating emissions of long-lived trace gases from a sparse global network of high-frequency observatories, using both a global Eulerian chemical transport model and Lagrangian particle dispersion ...

Manning, A. J.

451

WEDNESDAY: Secretary Chu to Host Live Chat on Clean Energy and Innovation |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Secretary Chu to Host Live Chat on Clean Energy and Secretary Chu to Host Live Chat on Clean Energy and Innovation WEDNESDAY: Secretary Chu to Host Live Chat on Clean Energy and Innovation January 24, 2011 - 12:00am Addthis WASHINGTON, D.C. - U.S. Energy Secretary Steven Chu will host the Department of Energy's inaugural "Energy Matters" online town hall on President Obama's clean energy and innovation agenda as a follow-up to the State of the Union. He will speak to and answer questions from an online and in-person audience. WHAT: Energy Secretary Steven Chu to host "Energy Matters" live chat WHEN: Wednesday, January 26, 2011 12:45 PM EST WHERE: U.S. Department of Energy, Small Auditorium energy.gov/livechat To learn more about the event, visit the Energy Blog. Media contact(s):

452

FISSION HALF LIVES OF FERMIUM ISOTOPES WITHIN SKYRME HARTREE-FOCK-BOGOLIUBOV THEORY  

Science Conference Proceedings (OSTI)

Nuclear fission barriers, mass parameters and spontaneous fission half lives of fermium isotopes calculated in a framework of the Skyrme Hartree-Fock-Bogoliubov model with the SkM* force are discussed. Zero-point energy corrections in the ground state are determined for each nucleus using the Gaussian overlap approximation of the generator coordinate method and in the cranking formalism. Results of spontaneous fission half lives are compared to experimental data.

Baran, A. [Maria Curie-Sklodowska University; Staszczak, Andrzej [ORNL; Nazarewicz, A. [Oak Ridge National Laboratory (ORNL)

2011-01-01T23:59:59.000Z

453

Structure, Function and Reconstitution of Antenna Complexes from Green Photosynthetic Bacteria  

DOE Green Energy (OSTI)

This project is concerned with the structure and function of the chlorosome antennas found in green photosynthetic bacteria. Chlorosomes are ellipsoidal structures attached to the cytoplasmic side of the inner cell membrane. These antenna complexes provide a very large absorption cross section for light capture. Evidence is overwhelming that the chlorosome represents a very different type of antenna from that found in any other photosynthetic system yet studied. It is now clear that chlorosomes do not contain traditional pigment-proteins, in which the pigments bind to specific sites on proteins. Instead, the chlorosome pigments are organized in vivo into pigment oligomers in which direct pigment-pigment interactions are of dominant importance. Our group has used a multidisciplinary approach to investigate this unique system, as well as the complexes that they directly interact with. Our work has included using model systems, numerous types of both steady-state and ultrafast spectroscopy, molecular biology, protein chemistry and X-ray crystallography. Details of our recent results using these approaches are given below and in the references. Numbers cited in the sections refer to DOE-sponsored publications that are listed below. Only publications dated 2001-2004 or later are included in this report. In addition to the primary literature reports, a comprehensive review of this area of research has been written as well as a commentary.

Robert E. Blankenship

2005-08-10T23:59:59.000Z

454

Quantification of the Antimicrobial Substances Produced by Lactic Acid Bacteria used as an Intervention to Inhibit Escherichia coli O157:H7 and Salmonella in vitro and on Fresh Spinach (Spinacia oleracea)  

E-Print Network (OSTI)

The metabolic activity of bacterial microorganisms may influence the growth and metabolic activities of other microbes that are present in any specific niche. Lactic acid bacteria (LAB) are antagonistic to some microbial pathogens by the metabolic production of compounds with antimicrobial activity. Consequently, investigators have measured the effects of those antimicrobials to inhibit specific pathogens. However, the mode(s) of action of LAB against foodborne pathogens on products and/or in broth is not completely understood. Therefore, the objectives of this research were to (i) determine the LAB dose required for inhibition of Escherichia coli O157:H7 and Salmonella enterica in vitro and on spinach, and (ii) identify and quantify the major antimicrobial substances synthesized by LAB as a function of postinoculation storage conditions. Assays were performed at 7 degrees C under aerobic conditions. The foodborne pathogens dose responses were assessed in a liquid microbiological medium (in vitro) and on spinach leaf surfaces. Different levels of foodborne pathogens and LAB cultures were used. The addition of LAB cultures did not reduce E. coli O157:H7 or Salmonella enterica populations when performed in vitro. However, when LAB cultures were sprayed on the surfaces of spinach leaves at 8.0 log10 CFU/g, there were significant reductions on E. coli O157:H7 of 1.62 and 0.73 log10 CFU/g (after 3 days) and on Salmonella enterica of 1.85 and 0.71 log10 CFU/g (after 6 days) for treatments inoculated with an initial level of 2.0 and 4.0 log10 CFU/g, respectively. After quantification of the antimicrobial compounds synthesized by LAB cultures, they were correlated against the population growth of targeted pathogens. The highest Llactic acid (3.71 plus/minus 0.14 micromoles/ml, day 12) and hydrogen peroxide (3.72 plus/minus 3.34 microM, day 6) production were obtained from the in vitro sample inoculated with 8.0 log10 CFU/ml of LAB and 0.0 log10 CFU/ml of pathogens. The highest bacteriocin production (0.1 plus/minus 0.01 mg/ml) was obtained from the in vitro sample with 8.0 log10 CFU/ml of LAB and 2.0 log10 CFU/ml of pathogens. In conclusion, the LAB cultures were able to produce detectable amounts of antimicrobials that may be used as intervention and/or sciencebased practice against foodborne pathogens by producers and the industry.

Calix Lara, Thelma

2011-12-01T23:59:59.000Z

455

Pitting corrosion behavior of 316L stainless steel in the media of sulphate-reducing and iron-oxidizing bacteria  

SciTech Connect

Pitting corrosion behavior of 316L SS was investigated in the presence of aerobic and anaerobic bacteria isolated from cooling water system in oil refinery using polarization measurement, electrochemical impedance spectroscopy, scanning electron microscopy examinations and energy dispersive spectrum analysis. The results show the corrosion potential (E{sub corr}), pitting potential (E{sub pit}) and polarization resistance (R{sub P}) of 316L SS had a distinct decrease in the presence of bacteria, in comparison with those observed in the sterile medium for the same exposure time interval. Micrometer-scale pitting was observed on the 316L SS surface in the presence of bacteria. The combination of SRB and IOB demonstrated higher corrosion rates than SRB or IOB alone. The synergy of 0.01 M NaCl + SRB + IOB yielded the highest corrosion rate. The synergies between the metal surface, abiotic corrosion products, chloride anion, and bacterial cells and their metabolic products increased the corrosion damage degree of the passive film and accelerated pitting propagation.

Xu Congmin [Xi'an Jiaotong University, Xi'an 710049 (China); Zhang Yaoheng [Xi'an Jiaotong University, Xi'an 710049 (China); Research and Technology Center of Lanzhou Oil Refinery Factory, PetroChina Company limited, Lanzhou, 730060 (China); Cheng Guangxu [Xi'an Jiaotong University, Xi'an 710049 (China)], E-mail: gxcheng@mail.xjtu.edu.cn; Zhu Wensheng [Research and Technology Center of Lanzhou Oil Refinery Factory, PetroChina Company limited, Lanzhou, 730060 (China)

2008-03-15T23:59:59.000Z

456

Relevance of the photosynthetic reaction center from purple bacteria to the structure of photosystem II  

SciTech Connect

Photosynthetic organisms are able to oxidize organic or inorganic compounds upon the absorption of light, and they use the extracted electron for the fixation of carbon dioxide. The most important oxidation product is oxygen due to the splitting of water. In eukaryotes these processes occur in photosystem II of chloroplasts. Among prokaryotes photosynthetic oxygen evolution is restricted to cyanobacteria and prochloron-type organisms. How water is split in the oxygen-evolving complex of photosystem II belongs to the most important question to be answered. The primary charge separation occurs in the reaction center of photosystem II. This reaction center is a complex consisting of peripheral and integral membrane proteins, several chlorophyll A molecules, two pheophytin A molecules, two and three plastoquinone molecules, and one non-heme iron atom. The location of the photosystem II reaction center is still a matter of debate. Nakatani et al. (l984) concluded from fluorescence measurements that a protein of apparent molecular weight 47,000 (CP47) is the apoprotein of the photosystem II reaction center. A different view emerged from work with the photosynthetic reaction centers from the purple bacteria. The amino acid sequence of the M subunit of the reaction center from Phodopseudomonas (Rps.) sphaeroides has sequence homologies with the D1 protein from spinach. A substantial amount of structural information can be obtained with the reaction center from Rhodopseudomonas viridis, which can be crystallized. Here the authors discuss the structure of the photosynthetic reaction center from the purple bacterium Rps. viridis and describe the role of those amino acids that are conserved between the bacterial and photosystem II reaction center.

Michel, H.; Deisenhofer, J.

1988-01-12T23:59:59.000Z

457

Electron spin polarization of the oxidized primary electron donor in reaction centers of photosynthetic purple bacteria  

SciTech Connect

Fast time-resolved EPR spectroscopy is used to study electron spin polarization (ESP) in perdeuterated native, Fe{sup 2+}-containing reaction centers (RCs) of photosynthetic purple bacteria. The spin-correlated radical pair-(SCRP) model previously used to simulate ESP observed in Fe-depleted RCs is extended to include the large anisotropy arising from the magnetic interactions between Fe{sup 2+} and the reduced primary electron-acceptor quinone (Q{sub A}{sup .-}), which results in different quantization axes from the P{sup .+} and the (Q{sub A}{sup .-}Fe{sup 2+}) spins. Using spectral simulations, it is shown that the ESP spectrum is solely due to the P{sup .+} part of the spin-correlated radical pair [P{sup .+}(Q{sub A}{sup .-}Fe{sup 2+})], whereas the rapid decay of the spin-polarized signal is due to spin-lattice relaxation of the (Q{sub A}{sup .-}Fe{sup 2+}) complex. The simulations are very sensitive to the relative orientation of the g matrices of P{sup .+} and (Q{sub A}{sup .-}Fe{sup 2+}). Using orientation II of the g matrix of the oxidized primary donor P{sup .+}, the orientation of the g matrix of (Q{sub A}{sup .-}Fe{sup 2+}) is assessed. Finally, it is shown that the ESP spectrum of perdeuterated native, Fe{sup 2+}-containing RCs of Rhodopseudomonas (Rps) viridis is virtually identical to the spectrum obtained for perdeuterated native Rhodobacter (Rb.) sphaeroides. 55 refs., 5 figs., 4 tabs.

Brink, J.S. van den; Gast, P.; Hoff, A.J. [Leiden Univ. (Netherlands)] [Leiden Univ. (Netherlands); Hermolle, T.E.P.; Hore, P.J. [Physical and Theoretical Chemistry Lab., Oxford (United Kingdom)] [Physical and Theoretical Chemistry Lab., Oxford (United Kingdom)

1996-02-08T23:59:59.000Z

458

WATCH LIVE: Secretary Chu at 10:45 am ET | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

WATCH LIVE: Secretary Chu at 10:45 am ET WATCH LIVE: Secretary Chu at 10:45 am ET WATCH LIVE: Secretary Chu at 10:45 am ET April 20, 2012 - 9:00am Addthis Amanda Scott Amanda Scott Former Managing Editor, Energy.gov This morning, at 10:45 am ET, we'll be streaming a live chat with Secretary Chu celebrating Earth Day. During the event, Secretary Chu will answer questions from you about the environmental and economic benefits of transitioning to a clean energy economy. If you haven't submitted a question yet, there's still time. We'll be taking questions throughout the live chat via email to newmedia@hq.doe.gov, on Facebook at Facebook.com/energygov, and via Twitter to Twitter.com/energy using the hashtag #AskEnergy. Send them now or during the event. Then tune in to Energy.gov/livechat at 10:45 am ET to watch.

459

Utilization of DNA as a Sole Source of Phosphorus, Carbon, and Energy by Shewanella spp.: Ecological and Physiological Implications for Dissimilatory Metal Reduction  

Science Conference Proceedings (OSTI)

As a constituent of dissolved organic matter, DNA may be consumed by microorganisms inhabiting various freshwater and marine environments. In this study, we demonstrate that dissolved extracellular DNA can serve as a sole source of carbon, energy, nitrogen, and phosphorus for microorganisms residing in the upper layer of Columbia River (WA, USA) water column as well as a sole source of phosphorus for the dissimilatory metal-reducing bacteria Shewanella oneidensis MR-1 and Geobacter sulfurreducens and for Bacillus subtilis ATCC 49760. Our results suggest that DNA assimilation by S. oneidensis is linked to the activity of Ca2+-dependent nuclease(s) and extracellular phosphatase(s). The ability to use DNA as the sole source of phosphorus may be of particular ecological advantage for microorganisms living under Fe(III)-reducing conditions where bioavailability of inorganic phosphate may be limited by the formation of vivianite [Fe3(PO4)28H20].

Pinchuk, Grigoriy E.; Ammons, Christine G.; Culley, David E.; Li, Shu-Mei; McLean, Jeffrey S.; Romine, Margaret F.; Nealson, Kenneth H.; Fredrickson, Jim K.; Beliaev, Alex S.

2008-02-15T23:59:59.000Z

460

#askEnergy: Live Twitter Chat with Home Energy Efficiency Experts |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

#askEnergy: Live Twitter Chat with Home Energy Efficiency Experts #askEnergy: Live Twitter Chat with Home Energy Efficiency Experts #askEnergy: Live Twitter Chat with Home Energy Efficiency Experts September 11, 2012 - 10:20am Q&A What questions do you have about improving your home's energy efficiency? Ask Us Addthis The Energy Department's home energy efficiency experts David Lee and Sam Rashkin will be answering your questions on ways to save energy and money at home. | Image courtesy of Sarah Gerrity. The Energy Department's home energy efficiency experts David Lee and Sam Rashkin will be answering your questions on ways to save energy and money at home. | Image courtesy of Sarah Gerrity. Rebecca Matulka Rebecca Matulka Digital Communications Specialist, Office of Public Affairs How can I participate? Tweet your questions to @ENERGY using #askEnergy.

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they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


461

Geek-Up[10.22.2010]: Exploring the CAVE, Electrical Links to Living Cells,  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Geek-Up[10.22.2010]: Exploring the CAVE, Electrical Links to Living Geek-Up[10.22.2010]: Exploring the CAVE, Electrical Links to Living Cells, and Energy Execs Geek-Up[10.22.2010]: Exploring the CAVE, Electrical Links to Living Cells, and Energy Execs October 22, 2010 - 1:55pm Addthis INL intern, Vanessa Gertman, shares her experiences in the CAVE Niketa Kumar Niketa Kumar Public Affairs Specialist, Office of Public Affairs At Idaho National Laboratory, engineers are walking into the core of nuclear reactors and rappelling down cliffs, all without ever leaving the office. Using a new 3D computer-assisted virtual environment (aka CAVE), INL researchers are literally walking into their data. In the CAVE, engineers use 3D goggles and a handheld controller that allows them to manipulate the data. The system tracks the movement of the user's head

462

#askEnergy: Live Twitter Chat with Home Energy Efficiency Experts |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

#askEnergy: Live Twitter Chat with Home Energy Efficiency Experts #askEnergy: Live Twitter Chat with Home Energy Efficiency Experts #askEnergy: Live Twitter Chat with Home Energy Efficiency Experts September 11, 2012 - 10:20am Q&A What questions do you have about improving your home's energy efficiency? Ask Us Addthis The Energy Department's home energy efficiency experts David Lee and Sam Rashkin will be answering your questions on ways to save energy and money at home. | Image courtesy of Sarah Gerrity. The Energy Department's home energy efficiency experts David Lee and Sam Rashkin will be answering your questions on ways to save energy and money at home. | Image courtesy of Sarah Gerrity. Rebecca Matulka Rebecca Matulka Digital Communications Specialist, Office of Public Affairs How can I participate? Tweet your questions to @ENERGY using #askEnergy.

463

Sharing Knowledge for a Low-Carbon Future: Zoellick and Chu in "live"  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Sharing Knowledge for a Low-Carbon Future: Zoellick and Chu in Sharing Knowledge for a Low-Carbon Future: Zoellick and Chu in "live" discussion Sharing Knowledge for a Low-Carbon Future: Zoellick and Chu in "live" discussion July 13, 2011 - 12:00am Addthis On Wednesday July 13, World Bank President Robert B. Zoellick and US Energy Secretary Steven Chu will discuss how technology and policy can help the world move toward a low-carbon future. Their half-hour discussion at the World Bank's Washington headquarters will be web-streamed live via the World Bank's external website. The Zoellick-Chu dialogue is part of an all-day gathering of high-level representatives from developing and developed countries, international institutions and think tanks. With more than 100 countries now designing low-carbon development plans, the calls for greater coherence and

464

Table HC1.2.2 Living Space Characteristics by Average Floorspace  

U.S. Energy Information Administration (EIA) Indexed Site

2 Living Space Characteristics by Average Floorspace, " 2 Living Space Characteristics by Average Floorspace, " " Per Housing Unit and Per Household Member, 2005" ,,"Average Square Feet" ," Housing Units (millions)" ,,"Per Housing Unit",,,"Per Household Member" "Living Space Characteristics",,"Total1","Heated","Cooled","Total1","Heated","Cooled" "Total",111.1,2033,1618,1031,791,630,401 "Total Floorspace (Square Feet)" "Fewer than 500",3.2,357,336,113,188,177,59 "500 to 999",23.8,733,667,308,343,312,144 "1,000 to 1,499",20.8,1157,1086,625,435,409,235 "1,500 to 1,999",15.4,1592,1441,906,595,539,339 "2,000 to 2,499",12.2,2052,1733,1072,765,646,400