National Library of Energy BETA

Sample records for bacteria microorganisms living

  1. Bacteria Marinobacter aquaeolei. Community Living. Microbes living in coral provide

    E-Print Network [OSTI]

    Bacteria Marinobacter aquaeolei. Community Living. Microbes living in coral provide nutrients Cleaning up. Bacteria are being tested for use as cleaning agents of toxic chemicals and pollutants in our.The big red and green dots (numbering about 1,000) are bacteria, and the very small background dots (about

  2. Response of Free-Living Nitrogen-Fixing Microorganisms to Land Use Change in the Amazon Rainforest

    E-Print Network [OSTI]

    Bohannan, Brendan

    ) of this greenhouse gas annually (1, 2). To absorb this enormous amount of CO2, a sig- nificant input of nitrogen (NResponse of Free-Living Nitrogen-Fixing Microorganisms to Land Use Change in the Amazon Rainforest-living nitrogen-fixing microorganisms (di- azotrophs) respond to deforestation in the Amazon rainforest, using

  3. Microorganism immobilization

    DOE Patents [OSTI]

    Compere, Alicia L. (Knoxville, TN); Griffith, William L. (Oak Ridge, TN)

    1981-01-01

    Live metabolically active microorganisms are immobilized on a solid support by contacting particles of aggregate material with a water dispersible polyelectrolyte such as gelatin, crosslinking the polyelectrolyte by reacting it with a crosslinking agent such as glutaraldehyde to provide a crosslinked coating on the particles of aggregate material, contacting the coated particles with live microorganisms and incubating the microorganisms in contact with the crosslinked coating to provide a coating of metabolically active microorganisms. The immobilized microorganisms have continued growth and reproduction functions.

  4. Method of separating bacteria from free living amoebae

    DOE Patents [OSTI]

    Tyndall, Richard L. (Clinton, TN)

    1994-01-01

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  5. Selective Association Between the Free-Living Nematode Acrobeloides maximus and Soil Bacteria

    E-Print Network [OSTI]

    Sedky, Sammy Farid

    2013-01-01

    from intracellular bacteria to multicellular eukaryotes.gene transfer between bacteria and animals. Trends inof the symbiotic-pathogenic bacteria Xenorhabdus spp. and

  6. A dose of bacteria early in life may help babies to live longer Sarah Staples. The Vancouver Sun Vancouver, B.C.:Aug 17, 2004. p. A6

    E-Print Network [OSTI]

    Seroude, Laurent

    A dose of bacteria early in life may help babies to live longer Sarah Staples. The Vancouver Sun Vancouver, B.C.:Aug 17, 2004. p. A6 Abstract (Article Summary) "We derive nutrients from bacteria, and many systems and organisms need bacteria to properly develop. Exposure to [bacteria] is required to train

  7. S. aureus bacteria : a new target of serum calcification activity

    E-Print Network [OSTI]

    Dy, Diane Jazmin

    2009-01-01

    and Antibiotics in Gram-Positive Bacteria and Mycobacteria.If mineralization of live bacteria is possible, differentof mineralization of live bacteria as a leukocyte response

  8. Mechanisms Regulating Mercury Bioavailability for Methylating Microorganisms in the Aquatic Environment: A Critical Review

    E-Print Network [OSTI]

    Mechanisms Regulating Mercury Bioavailability for Methylating Microorganisms in the Aquatic by anaerobic bacteria. In this Review, we evaluate the current state of knowledge regarding the mechanisms the uptake of inorganic mercury to these microorganisms. Our understanding of the mechanisms behind

  9. RESEARCH ARTICLE Fluid dynamics of self-propelled microorganisms,

    E-Print Network [OSTI]

    Goldstein, Raymond E.

    RESEARCH ARTICLE Fluid dynamics of self-propelled microorganisms, from individuals to concentrated non-pathogenic soil bacteria are rod-shaped (Fig. 1). Their length ranges from 2 to 8 lm, depending

  10. RESEARCH ARTICLE Fluid dynamics of self-propelled microorganisms,

    E-Print Network [OSTI]

    Cortez, Ricardo

    RESEARCH ARTICLE Fluid dynamics of self-propelled microorganisms, from individuals to concentrated00348-007-0387-y #12;Individual cells of these generally non-pathogenic soil bacteria are rod

  11. Cellulolytic Microorganisms from Thermal Environments

    SciTech Connect (OSTI)

    Vishnivetskaya, Tatiana A [ORNL; Raman, Babu [ORNL; Phelps, Tommy Joe [ORNL; Podar, Mircea [ORNL; Elkins, James G [ORNL

    2012-01-01

    Thermal, anaerobic environments rich in decaying plant material are a potential source of novel cellulolytic bacteria. Samples collected from geothermal aquifers in the Yellowstone National Park (YNP) were used to select for cellulolytic thermophiles. Laboratory enrichments on dilute-acid pretreated plant biomass (switchgrass, Populus), and crystalline cellulose (Avicel) resulted in the isolation of 247 environmental clones. The majority of individual clones were affiliated with the cellulolytic bacteria of phylum Firmicutes, followed by xylanolytic and saccharolytic members of the phylum Dictyoglomi. Among the Firmicutes, the clones were affiliated with the genera Caldicellulosiruptor (54.4%), Caloramator (11.5%), Thermoanaerobacter (8.8%), Thermovenabulum (4.1%), and Clostridium (2.0%). From established anaerobic thermophilic enrichments a total of 81 single strains of the genera Caldicellulosiruptor (57%) and Thermoanaerobacter (43%) were isolated. With continuous flow enrichment on Avicel, increases in the relative abundance of Caloramator sp. was observed over clones detected from the Caldicellulosiruptor. Complex communities of interacting microorganisms bring about cellulose decomposition in nature, therefore using up-to-date approaches may yield novel cellulolytic microorganisms with high activity and a rapid rate of biomass conversion to biofuels.

  12. Response of Prochlorococcus ecotypes to co-culture with diverse marine bacteria

    E-Print Network [OSTI]

    Sher, Daniel

    Interactions between microorganisms shape microbial ecosystems. Systematic studies of mixed microbes in co-culture have revealed widespread potential for growth inhibition among marine heterotrophic bacteria, but similar ...

  13. Microorganisms for producing organic acids

    DOE Patents [OSTI]

    Pfleger, Brian Frederick; Begemann, Matthew Brett

    2014-09-30

    Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.

  14. Ferric hydroxide and ferric hydroxysulfate precipitation by bacteria in an acid mine drainage lagoon

    E-Print Network [OSTI]

    Konhauser, Kurt

    Ferric hydroxide and ferric hydroxysulfate precipitation by bacteria in an acid mine drainage communities growing in an acid mine drainage lagoon sediment has confirmed that microorganisms were also: Ferrihydrite; Ferric hydroxysulfate; Bacteria; Biomineralization; Acid mine drainage Contents 1. Introduction

  15. Proteolysis in hyperthermophilic microorganisms

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ward, Donald E.; Shockley, Keith R.; Chang, Lara S.; Levy, Ryan D.; Michel, Joshua K.; Conners, Shannon B.; Kelly, Robert M.

    2002-01-01

    Proteases are found in every cell, where they recognize and break down unneeded or abnormal polypeptides or peptide-based nutrients within or outside the cell. Genome sequence data can be used to compare proteolytic enzyme inventories of different organisms as they relate to physiological needs for protein modification and hydrolysis. In this review, we exploit genome sequence data to compare hyperthermophilic microorganisms from the euryarchaeotal genusPyrococcus, the crenarchaeoteSulfolobus solfataricus, and the bacteriumThermotoga maritima. An overview of the proteases in these organisms is given based on those proteases that have been characterized and on putative proteases that have been identified from genomicmore »sequences, but have yet to be characterized. The analysis revealed both similarities and differences in the mechanisms utilized for proteolysis by each of these hyperthermophiles and indicated how these mechanisms relate to proteolysis in less thermophilic cells and organisms.« less

  16. Luminal Bacteria Recruit CD103+ Dendritic Cells

    E-Print Network [OSTI]

    Immunity Article Luminal Bacteria Recruit CD103+ Dendritic Cells into the Intestinal Epithelium@weizmann.ac.il http://dx.doi.org/10.1016/j.immuni.2013.01.009 SUMMARY CD103+ dendritic cells (DCs) carry bacteria from bacteria or presenting bacterial antigens in mesen- tery lymph nodes. We used 2-photon microscopy in live

  17. Chemosensing in microorganisms to practical biosensors

    E-Print Network [OSTI]

    Surya K. Ghosh; Tapanendu Kundu; Anirban Sain

    2012-10-25

    Microorganisms like bacteria can sense concentration of chemo-attractants in its medium very accurately. They achieve this through interaction between the receptors on their cell surface and the chemo-attractant molecules (like sugar). But the physical processes like diffusion set some limits on the accuracy of detection which was discussed by Berg and Purcell in the late seventies. We have a re-look at their work in order to assess what insight it may offer towards making efficient, practical biosensors. We model the functioning of a typical biosensor as a reaction-diffusion process in a confined geometry. Using available data first we characterize the system by estimating the kinetic constants for the binding/unbinding reactions between the chemo-attractants and the receptors. Then we compute the binding flux for this system which Berg and Purcell had discussed. But unlike in microorganisms where the interval between successive measurements determines the efficiency of the nutrient searching process, it turns out that biosensors depend on long time properties like signal saturation time which we study in detail. We also develop a mean field description of the kinetics of the system.

  18. Digital holographic imaging of microorganisms

    E-Print Network [OSTI]

    Wolf, Michael Trevor

    2006-01-01

    Imaging aquatic microorganisms in 3D space is of interest to biologists and ocean scientists seeking to understand the behavior of these organisms in their natural environments. In this research, digital holographic imaging ...

  19. 1529-6466/03/0054-0004$05.00 Biologically Induced Mineralization by Bacteria

    E-Print Network [OSTI]

    Dove, Patricia M.

    1529-6466/03/0054-0004$05.00 Biologically Induced Mineralization by Bacteria Richard B. Frankel 50011 U.S.A. INTRODUCTION Bacteria are small, prokaryotic, microorganisms that are ubiquitous in surface) in the biological taxonomic hierarchy, the Bacteria and the Archaea. They exhibit remarkable diversity both

  20. Immunology Taught by Bacteria

    E-Print Network [OSTI]

    Vance, Russell E.

    2010-01-01

    purified PAMPs or killed bacteria. Cytosolic RNA sensors do2 Immunology Taught by Bacteria Russell E. Vance Received:multiple pathogenic bacteria. Conclusion Thus, immunology

  1. The identification and biogeochemical interpretation of fossil magnetotactic bacteria

    E-Print Network [OSTI]

    Kirschvink, Joseph L.

    The identification and biogeochemical interpretation of fossil magnetotactic bacteria Robert E. Available online 14 August 2007. Abstract Magnetotactic bacteria, which most commonly live within the oxic, specifically magnetite or greigite. The crystals cause the bacteria to orient themselves passively with respect

  2. Leachability of salmonella and fecal pollution indicator bacteria through soil 

    E-Print Network [OSTI]

    Fehrmann, Robert Clinton

    1977-01-01

    of Department (Member) / /. ' Member) August 1977 74M3- ABSTRACT Leachability of Salmonella and Fecal Pollution Indicator Bacteria through Soil. (August 1977) Robert Clinton Fehrmann, B. S. , Texas AM University Chairman of Advisory Committee: Dr... microorganisms to be leached through soil, columns :ere filled with different soils and inoculs. ted with suspensions of fecal bacteria. Dif er ences in bacterial movement within a particular sni I, and bacterial movement between different types of soils...

  3. Cellulase producing microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1997-12-30

    Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  4. Cellulase producing microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig (Lenoir City, TN)

    1997-01-01

    Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  5. Emerging and opportunistic diseases are caused by a microorganism invading a new

    E-Print Network [OSTI]

    Gomulkiewicz, Richard

    Emerging and opportunistic diseases are caused by a microorganism invading a new habitat, either meningitis). Often, the pathogen can live for many generations in the new habitat and, as natural selection be applied to most bacterial pathogens and suggest a phylogeny-based method to search for genes undergoing

  6. Contribution of microorganisms to corrosion

    SciTech Connect (OSTI)

    Thorp, K.E.G.; Crasto, A.S. [Univ. of Dayton Research Inst., OH (United States); Gu, J.D.; Mitchell, R. [Harvard Univ., Cambridge, MA (United States). Div. of Applied Sciences

    1997-08-01

    Current metal primers utilized by the US Air Force contain chromates to inhibit corrosion of the underlying metal. These chromates are both highly toxic and carcinogenic and pose a severe health risk to personnel involved in their application, stripping and disposal. Environmentally-friendly primers with chromate replacements have historically performed poorly with respect to corrosion inhibition. The purpose of this study was to investigate the interaction of chromates with microorganisms in an environment not traditionally associated with biologically-enhanced corrosion to determine if corrosion inhibition by a chromate pigment is, in part, through its action as a biocide. Inoculation of panels which had been coated with a nonchromated primer prior to salt fog exposure and storage in humid conditions resulted in a significant growth of filiform corrosion around a scribe mark. The presence of chromate in the primer severely limited the formation of this corrosion. Likewise, in the absence of the inoculation procedure, the extent of corrosion was strongly diminished. These results suggest that the chromate may be acting as a biocide to limit corrosion which is enhanced by the presence of biological activity.

  7. Bacteria isolated from amoebae/bacteria consortium

    DOE Patents [OSTI]

    Tyndall, R.L.

    1995-05-30

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  8. Bacteria isolated from amoebae/bacteria consortium

    DOE Patents [OSTI]

    Tyndall, Richard L. (Clinton, TN)

    1995-01-01

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  9. Bacteria may hold key to longevity Sarah Staples. Times -Colonist Victoria, B.C.:Aug 17, 2004. p. C11

    E-Print Network [OSTI]

    Seroude, Laurent

    Bacteria may hold key to longevity Sarah Staples. Times - Colonist Victoria, B.C.:Aug 17, 2004. p between infant fruit flies and the bacteria that live on them, have found some of the strongest evidence yet that a dose of bacteria early in life, far from harming babies, may actually help them live longer

  10. AOML Keynotes July-August 2004 Students Discover Microscopic World of Marine Bacteria

    E-Print Network [OSTI]

    Goodwin, Kelly D.

    AOML Keynotes July-August 2004 Students Discover Microscopic World of Marine Bacteria AOML microbiologist Kelly Goodwin helped teach young girls about the invisible world of marine bacteria at a recent support the growth of marine bacteria, microscopic life forms living in the ocean. They were taught how

  11. Nano-optical Trapping of Rayleigh Particles and Escherichia coli Bacteria

    E-Print Network [OSTI]

    Nano-optical Trapping of Rayleigh Particles and Escherichia coli Bacteria with Resonant Optical demonstrate a novel optical trapping scheme that allows us to hold living Escherichia coli bacteria bacteria are trapped simultaneously with their orientation fixed by the asymmetry of the antennas

  12. Live Status

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    For Users Live Status Queue Look Classic Queue Look Scheduled Outages Outage Log Science Gateway Status Login Node Status Filesystem Status My NERSC Move to CRT Getting Started...

  13. Microfluidic passive samplers for in situ collection of live aquatic protists

    E-Print Network [OSTI]

    Shor, Leslie McCabe

    Microfluidic passive samplers for in situ collection of live aquatic protists Grant M. Bouchillon of microfluidic passive samplers for the collection of live protists from natural aquatic habitats. Microfluidic microfluidic observation galleries. In field experiments, live protists and other microorganisms were collected

  14. Bacteria TMDL Projects 

    E-Print Network [OSTI]

    Wythe, Kathy

    2007-01-01

    stream_source_info Bacteria TMDL projects.pdf.txt stream_content_type text/plain stream_size 2550 Content-Encoding ISO-8859-1 stream_name Bacteria TMDL projects.pdf.txt Content-Type text/plain; charset=ISO-8859-1 tx H2O... of the projects are listed below. ? Peach CreekWater Quality Improvement Project ? Monitoring and Educational Programs Focused on Bacteria and Nutrient Runoff on Dairy Operations in the LeonWatershed ? Development of the Plum CreekWPP ? Impact of Proper...

  15. Bacteria in shear flow

    E-Print Network [OSTI]

    Marcos, Ph.D. Massachusetts Institute of Technology

    2011-01-01

    Bacteria are ubiquitous and play a critical role in many contexts. Their environment is nearly always dynamic due to the prevalence of fluid flow: creeping flow in soil, highly sheared flow in bodily conduits, and turbulent ...

  16. Quantification of the Antimicrobial Substances Produced by Lactic Acid Bacteria used as an Intervention to Inhibit Escherichia coli O157:H7 and Salmonella in vitro and on Fresh Spinach (Spinacia oleracea) 

    E-Print Network [OSTI]

    Calix Lara, Thelma

    2012-02-14

    The metabolic activity of bacterial microorganisms may influence the growth and metabolic activities of other microbes that are present in any specific niche. Lactic acid bacteria (LAB) are antagonistic to some microbial ...

  17. Electrosynthesis of Organic Compounds from Carbon Dioxide Is Catalyzed by a Diversity of Acetogenic Microorganisms

    SciTech Connect (OSTI)

    Nevin, KP; Hensley, SA; Franks, AE; Summers, ZM; Ou, JH; Woodard, TL; Snoeyenbos-West, OL; Lovley, DR

    2011-04-20

    Microbial electrosynthesis, a process in which microorganisms use electrons derived from electrodes to reduce carbon dioxide to multicarbon, extracellular organic compounds, is a potential strategy for capturing electrical energy in carbon-carbon bonds of readily stored and easily distributed products, such as transportation fuels. To date, only one organism, the acetogen Sporomusa ovata, has been shown to be capable of electrosynthesis. The purpose of this study was to determine if a wider range of microorganisms is capable of this process. Several other acetogenic bacteria, including two other Sporomusa species, Clostridium ljungdahlii, Clostridium aceticum, and Moorella thermoacetica, consumed current with the production of organic acids. In general acetate was the primary product, but 2-oxobutyrate and formate also were formed, with 2-oxobutyrate being the predominant identified product of electrosynthesis by C. aceticum. S. sphaeroides, C. ljungdahlii, and M. thermoacetica had high (> 80%) efficiencies of electrons consumed and recovered in identified products. The acetogen Acetobacterium woodii was unable to consume current. These results expand the known range of microorganisms capable of electrosynthesis, providing multiple options for the further optimization of this process.

  18. Quantum superposition, entanglement, and state teleportation of a microorganism on an electromechanical oscillator

    E-Print Network [OSTI]

    Li, Tongcang

    2015-01-01

    Schr\\"odinger's thought experiment to prepare a cat in a superposition of both alive and dead states reveals profound consequences of quantum mechanics and has attracted enormous interests. Here we propose a straightforward method to create quantum superposition states of a living microorganism by putting a small bacterium on top of an electromechanical oscillator. Our proposal is based on recent developments that the center-of-mass oscillation of a 15-$\\mu$m-diameter aluminium membrane has been cooled to its quantum ground state [Nature 475, 359 (2011)], and entangled with a microwave field [Science, 342, 710 (2013)]. A microorganism with a mass much smaller than the mass of the electromechanical membrane will not significantly affect the quality factor of the membrane and can be cooled to the quantum ground state together with the membrane. Quantum superposition and teleportation of its center-of-mass motion state can be realized with the help of superconducting microwave circuits. More importantly, the int...

  19. Storing data encoded DNA in living organisms

    DOE Patents [OSTI]

    Wong; Pak C. (Richland, WA), Wong; Kwong K. (Sugar Land, TX), Foote; Harlan P. (Richland, WA)

    2006-06-06

    Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

  20. Apparatus and method for the desulfurization of petroleum by bacteria

    DOE Patents [OSTI]

    Lizama, Hector M. (Knoxville, TN); Scott, Timothy C. (Knoxville, TN); Scott, Charles D. (Oak Ridge, TN)

    1995-01-01

    A method for treating petroleum with anaerobic microorganisms acting as biocatalysts that can remove sulfur atoms from hydrocarbon molecules, under anaerobic conditions, and then convert the sulfur atoms to hydrogen sulfide. The microorganisms utilized are from the family known as the "Sulfate Reducing Bacteria." These bacteria generate metabolic energy from the oxidation of organic compounds, but use oxidized forms of sulfur as an electron acceptor. Because the biocatalyst is present in the form of bacteria in an aqueous suspension, whereas the reacting substrate consists of hydrocarbon molecules in an organic phase, the actual desulfurization reaction takes place at the aqueous-organic interphase. To ensure adequate interfacial contacting and mass transfer, a biphasic electrostatic bioreactor system is utilized. The bioreactor is utilized to disperse and recoalesce a biocatalyst contained in the aqueous liquid phase into the organic liquid phase containing the sulfur. High-intensity electrical fields rupture the aqueous drops into a plurality of microdroplets and induce continuous coalescence and redispersion as the microdroplets travel through the organic phase, thus increasing surface area. As the aqueous microdroplets progress through the organic phase, the biocatalyst then reacts with the sulfur to produce hydrogen sulfide which is then removed from the bioreactor. The organic liquid, now free of the sulfur, is ready for immediate use or further processing.

  1. Apparatus and method for the desulfurization of petroleum by bacteria

    DOE Patents [OSTI]

    Lizama, H.M.; Scott, T.C.; Scott, C.D.

    1995-10-17

    A method is described for treating petroleum with anaerobic microorganisms acting as biocatalysts that can remove sulfur atoms from hydrocarbon molecules, under anaerobic conditions, and then convert the sulfur atoms to hydrogen sulfide. The microorganisms utilized are from the family known as the ``Sulfate Reducing Bacteria``. These bacteria generate metabolic energy from the oxidation of organic compounds, but use oxidized forms of sulfur as an electron acceptor. Because the biocatalyst is present in the form of bacteria in an aqueous suspension, whereas the reacting substrate consists of hydrocarbon molecules in an organic phase, the actual desulfurization reaction takes place at the aqueous-organic interphase. To ensure adequate interfacial contacting and mass transfer, a biphasic electrostatic bioreactor system is utilized. The bioreactor is utilized to disperse and recoalesce a biocatalyst contained in the aqueous liquid phase into the organic liquid phase containing the sulfur. High-intensity electrical fields rupture the aqueous drops into a plurality of microdroplets and induce continuous coalescence and redispersion as the microdroplets travel through the organic phase, thus increasing surface area. As the aqueous microdroplets progress through the organic phase, the biocatalyst then reacts with the sulfur to produce hydrogen sulfide which is then removed from the bioreactor. The organic liquid, now free of the sulfur, is ready for immediate use or further processing. 5 figs.

  2. Engineered microorganisms capable of producing target compounds under anaerobic conditions

    DOE Patents [OSTI]

    Buelter, Thomas (Denver, CO); Meinhold, Peter (Denver, CO); Feldman, Reid M. Renny (San Francisco, CA); Hawkins, Andrew C. (Parker, CO); Urano, Jun (Irvine, CA); Bastian, Sabine (Pasadena, CA); Arnold, Frances (La Canada, CA)

    2012-01-17

    The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

  3. Use of Disinfectants and Cleaners to Reduce Bacteria on Poultry Transportation Coops with a Compressed Air Foam System 

    E-Print Network [OSTI]

    Hinojosa-Garza, Carolee A.

    2013-05-24

    company?s Hazard Analysis and Critical Control Points (HACCP) because a large amount of this bacteria be an indicator of improper sanitation. Cleaning and disinfection programs for this microorganism are being set by USDA and are being improved upon... Salmonella and aerobic bacteria, which could reduce contamination of carcasses. A recent literature search suggests that there are few procedures on how to clean and sanitize transport coops, this may be because it is currently not a requirement...

  4. Cargo delivery into gram-negative bacteria via enterobactin uptake machinery

    E-Print Network [OSTI]

    Zheng, Tengfei

    2014-01-01

    Chapter 1. Introduction to Iron Homeostasis and Siderophores Iron is an essential nutrient for almost all living organisms. This Chapter presents an overview of iron homeostasis in human and bacteria, as well as the biology ...

  5. Culture of Bacteria 

    E-Print Network [OSTI]

    Unknown

    2011-08-17

    site located in the lungs of an animal model, thereby enabling detection of fluorescent bacteria during the early stages of infection. In this thesis, I present a contact probe fiber bundle fluorescence micro-endoscope with a range of LED based...

  6. Bioprocessing of lignite coals using reductive microorganisms

    SciTech Connect (OSTI)

    Crawford, D.L.

    1992-03-29

    In order to convert lignite coals into liquid fuels, gases or chemical feedstock, the macromolecular structure of the coal must be broken down into low molecular weight fractions prior to further modification. Our research focused on this aspect of coal bioprocessing. We isolated, characterized and studied the lignite coal-depolymerizing organisms Streptomyces viridosporus T7A, Pseudomonas sp. DLC-62, unidentified bacterial strain DLC-BB2 and Gram-positive Bacillus megaterium strain DLC-21. In this research we showed that these bacteria are able to solubilize and depolymerize lignite coals using a combination of biological mechanisms including the excretion of coal solublizing basic chemical metabolites and extracellular coal depolymerizing enzymes.

  7. Quantum superposition, entanglement, and state teleportation of a microorganism on an electromechanical oscillator

    E-Print Network [OSTI]

    Tongcang Li; Zhang-Qi Yin

    2015-09-12

    Schr\\"odinger's thought experiment to prepare a cat in a superposition of both alive and dead states reveals profound consequences of quantum mechanics and has attracted enormous interests. Here we propose a straightforward method to create quantum superposition states of a living microorganism by putting a small bacterium on top of an electromechanical oscillator. Our proposal is based on recent developments that the center-of-mass oscillation of a 15-$\\mu$m-diameter aluminium membrane has been cooled to its quantum ground state [Nature 475, 359 (2011)], and entangled with a microwave field [Science, 342, 710 (2013)]. A microorganism with a mass much smaller than the mass of the electromechanical membrane will not significantly affect the quality factor of the membrane and can be cooled to the quantum ground state together with the membrane. Quantum superposition and teleportation of its center-of-mass motion state can be realized with the help of superconducting microwave circuits. More importantly, the internal states of a microorganism, such as the electron spin of a glycine radical, can be prepared in a quantum superposition state and entangled with its center-of-mass motion. Our proposal can be realized with state-of-art technologies. The proposed setup is also a quantum-limited magnetic resonance force microscope (MRFM) that not only can detect the existence of an electron spin, but also can coherently manipulate and detect the quantum state of the spin.

  8. Non-indigenous microorganisms in the Antarctic: assessing the risks

    E-Print Network [OSTI]

    Vincent, Warwick F.

    Non-indigenous microorganisms in the Antarctic: assessing the risks Don A. Cowan1 , Steven L. Chown wastes at these sites, little has been done to prevent the introduction of non-indigenous microorganisms largely restricted to the impacts of human and animal pathogens (including viruses) [7,8,13] and organisms

  9. Dynamic Clustering in Suspension of Motile Bacteria

    E-Print Network [OSTI]

    Xiao Chen; Xiang Yang; Mingcheng Yang; Hepeng Zhang

    2015-09-22

    Bacteria suspension exhibits a wide range of collective phenomena arising from interactions between individual cells. Here we show Serratia marcescens cells near an air-liquid interface spontaneously aggregate into dynamic clusters through surface-mediated hydrodynamic interactions. These long-lived clusters translate randomly and rotate in the counter-clockwise direction; they continuously evolve, merge with others and split into smaller ones. Measurements indicate that long-ranged hydrodynamic interactions have strong influences on cluster properties. Bacterial clusters change material and fluid transport near the interface and hence may have environmental and biological consequences.

  10. Dynamic Clustering in Suspension of Motile Bacteria

    E-Print Network [OSTI]

    Chen, Xiao; Yang, Mingcheng; Zhang, Hepeng

    2015-01-01

    A bacteria suspension exhibits a wide range of collective phenomena arsing from interactions between individual cells. Here we show that Serratia marcescens cells near an air-liquid interface spontaneously aggregate into dynamic clusters through surface-mediated hydrodynamic interactions. These long-lived clusters translate randomly and rotate in the counter-clockwise direction; they continuously evolve, merge with others and split into smaller ones. The observed cluster dynamics is qualitatively reproduced by a numerical model of self-propelled particles that interact via pair-wise forces extracted from hydrodynamic calculations. Bacterial clusters change material and fluid transport near the interface and hence may have environmental and biological consequences.

  11. Self-engineering capabilities of bacteria

    E-Print Network [OSTI]

    Ben-Jacob, Eshel; Levine, Herbert

    2006-01-01

    Hellingwerf, K. J. 2005 Bacteria observations: a rudimentaryCommunication among oral bacteria. Microbiol. Mol. Biol.A. & Dworkin, M. 1997 Bacteria as multicellular organisms.

  12. Genomic potential for polysaccharide deconstruction in bacteria

    E-Print Network [OSTI]

    Berlemont, R; Martiny, AC; Martiny, AC

    2015-01-01

    Identification of cellulolytic bacteria in soil by stablepotential cellulases in bacteria. Appl Environ Microbiol 79:chitin catabolism in marine bacteria. Biochim Biophys Acta

  13. Phylogenetic Distribution of Potential Cellulases in Bacteria

    E-Print Network [OSTI]

    Berlemont, R.; Martiny, A. C

    2012-01-01

    cellulose production in bacteria. Res. Microbiol. 153:205–carbohydrate metabo- lism in bacteria. Microbiol. Mol. Biol.2006. Interac- tions of bacteria and fungi on decomposing

  14. STORMWATER MANAGEMENT MEASURES AND FECAL INDICATOR BACTERIA

    E-Print Network [OSTI]

    STORMWATER MANAGEMENT MEASURES AND FECAL INDICATOR BACTERIA BY ROBERT A. WILDEY BA, New College............................................................................................................. 1 Regulatory Limits for Indicator Bacteria................................................................ 2 Indicator Bacteria in the Environment

  15. News and Research Good Bacteria

    E-Print Network [OSTI]

    West, Stuart

    News and Research Good Bacteria Part 2 Article 13 Click here for Probiotics Basics Cooperation Is A No-brainer For Symbiotic Bacteria 9-4-2003 Humans may learn cooperation in kindergarten, but what about bacteria, whose behavior is preprogrammed by their DNA? Some legume plants, which rely

  16. Fuel from Bacteria, CO2, Water, and Solar Energy: Engineering a Bacterial Reverse Fuel Cell

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: Harvard is engineering a self-contained, scalable Electrofuels production system that can directly generate liquid fuels from bacteria, carbon dioxide (CO2), water, and sunlight. Harvard is genetically engineering bacteria called Shewanella, so the bacteria can sit directly on electrical conductors and absorb electrical current. This current, which is powered by solar panels, gives the bacteria the energy they need to process CO2 into liquid fuels. The Harvard team pumps this CO2 into the system, in addition to water and other nutrients needed to grow the bacteria. Harvard is also engineering the bacteria to produce fuel molecules that have properties similar to gasoline or diesel fuel—making them easier to incorporate into the existing fuel infrastructure. These molecules are designed to spontaneously separate from the water-based culture that the bacteria live in and to be used directly as fuel without further chemical processing once they’re pumped out of the tank.

  17. Potential of Diazorphic, Endophytic Bacteria Associated with...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Diazorphic, Endophytic Bacteria Associated with Sugarcane for Energycane Production Potential of Diazorphic, Endophytic Bacteria Associated with Sugarcane for Energycane...

  18. Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism

    DOE Patents [OSTI]

    Gaddy, James L. (Fayetteville, AR); Clausen, Edgar C. (Fayetteville, AR)

    1992-01-01

    A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H.sub.2 O and/or CO.sub.2 and H.sub.2 in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate.

  19. Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism

    DOE Patents [OSTI]

    Gaddy, J.L.; Clausen, E.C.

    1992-12-22

    A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H[sub 2]O and/or CO[sub 2] and H[sub 2] in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate. 3 figs.

  20. Biofuels from Solar Energy and Bacteria: Electrofuels Via Direct Electron Transfer from Electrodes to Microbes

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: UMass is feeding renewable electricity to bacteria to provide the microorganisms with the energy they need to turn carbon dioxide (CO2) directly into liquid fuels. UMass’ energy-to-fuels conversion process is anticipated to be more efficient than current biofuels approaches in part because this process will leverage the high efficiency of photovoltaics to convert solar energy into electricity. UMass is using bacteria already known to produce biofuel from electric current and CO2 and working to increase the amount of electric current those microorganisms will accept and use for biofuels production. In collaboration with scientists at University of California, San Diego, the UMass team is also investigating the use of hydrogen sulfide as a source of energy to power biofuel production.

  1. Selective microorganism concentration using a dielectrophoresis-based microfabricated device

    E-Print Network [OSTI]

    Pucha?a, Katarzyna Anna

    2007-01-01

    Detection of pathogenic microorganisms is a significant challenge in medicine, environmental protection and biological threat safety because samples are often contaminated. This work presents a method of separating bacterial ...

  2. Electrical characterization of micro-organisms using microfabricated devices

    E-Print Network [OSTI]

    Bashir, Rashid

    . Kosari and G. Vasmatzis Mayo Clinic, Rochester, Minnesota 55905 A. Bhunia Department of Food Sciences by the micro-organisms when traversing through the pore. © 2002 American Vacuum Society. DOI: 10

  3. Microorganisms having enhanced tolerance to inhibitors and stress

    DOE Patents [OSTI]

    Brown, Steven D.; Yang, Shihui

    2014-07-29

    The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.

  4. Microorganisms having enhanced resistance to acetate and methods of use

    DOE Patents [OSTI]

    Brown, Steven D; Yang, Shihui

    2014-10-21

    The present invention provides isolated or genetically modified strains of microorganisms that display enhanced resistance to acetate as a result of increased expression of a sodium proton antiporter. The present invention also provides methods for producing such microbial strains, as well as related promoter sequences and expression vectors. Further, the present invention provides methods of producing alcohol from biomass materials by using microorganisms with enhanced resistance to acetate.

  5. Can entropy save bacteria?

    E-Print Network [OSTI]

    Suckjoon Jun

    2008-08-29

    This article presents a physical biology approach to understanding organization and segregation of bacterial chromosomes. The author uses a "piston" analogy for bacterial chromosomes in a cell, which leads to a phase diagram for the organization of two athermal chains confined in a closed geometry characterized by two length scales (length and width). When applied to rod-shaped bacteria such as Escherichia coli, this phase diagram predicts that, despite strong confinement, duplicated chromosomes will demix, i.e., there exists a primordial physical driving force for chromosome segregation. The author discusses segregation of duplicating chromosomes using the concentric-shell model, which predicts that newly synthesized DNA will be found in the periphery of the chromosome during replication. In contrast to chromosomes, these results suggest that most plasmids will be randomly distributed inside the cell because of their small sizes. An active partitioning system is therefore required for accurate segregation of low-copy number plasmids. Implications of these results are also sketched, e.g., on the role of proteins, segregation mechanisms for bacteria of diverse shapes, cell cycle of an artificial cell, and evolution.

  6. Bioengineering and Coordination of Regulatory Networks and Intracellular Complexes to Maximize Hydrogen Production by Phototrophic Microorganisms

    SciTech Connect (OSTI)

    Tabita, F. Robert [The Ohio State University] [The Ohio State University

    2013-07-30

    In this study, the Principal Investigator, F.R. Tabita has teemed up with J. C. Liao from UCLA. This project's main goal is to manipulate regulatory networks in phototrophic bacteria to affect and maximize the production of large amounts of hydrogen gas under conditions where wild-type organisms are constrained by inherent regulatory mechanisms from allowing this to occur. Unrestrained production of hydrogen has been achieved and this will allow for the potential utilization of waste materials as a feed stock to support hydrogen production. By further understanding the means by which regulatory networks interact, this study will seek to maximize the ability of currently available “unrestrained” organisms to produce hydrogen. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Moreover, due to their great metabolic versatility, such organisms highly regulate these processes in the cell and since virtually all such capabilities are dispensable, excellent experimental systems to study aspects of molecular control and biochemistry/physiology are available.

  7. Living a Sustainable Future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    solve the energy crisis through biological methods, including genetically engineering algae and cyanobacteria. Create a Sustainable Future: Living Living a Sustainable Future How...

  8. Physical and Chemical Factors Influencing the Transport and Fate of Microorganisms in Soils With Preferential Flow

    E-Print Network [OSTI]

    Wang, Yusong

    2013-01-01

    water resources from pathogenic microorganisms and contaminants associated with the colloidal phase, and to optimize bioremediation

  9. Expansion of the Genomic Encyclopedia of Bacteria and Archaea

    SciTech Connect (OSTI)

    Rinke, Christian; Sczyrba, Alex; Malfatti, Stephanie; Lee, Janye; Cheng, Jan-Fang; Stepanauskas, Ramunas; Eisen, Jonathan A.; Hallam, Steven; Inskeep, William P.; Hedlund, Brian P.; Sievert, Stefan M.; Liu, Wen-Tso; Tsiamis, George; Hugenholtz, Philip; Woyke, Tanja

    2011-03-20

    To date the vast majority of bacterial and archaeal genomes sequenced are of rather limited phylogenetic diversity as they were chosen based on their physiology and/ or medical importance. The Genomic Encyclopedia of Bacteria and Archaea (GEBA) project (Wu et al. 2009) is aimed to systematically filling the gaps of the tree of life with phylogenetically diverse reference genomes. However more than 99percent of microorganisms elude current culturing attempts, severely limiting the ability to recover complete or even partial genomes of these largely mysterious species. These limitations gave rise to the GEBA uncultured project. Here we propose to use single cell genomics to massively expand the Genomic Encyclopedia of Bacteria and Archaea by targeting 80 single cell representatives of uncultured candidate phyla which have no or very few cultured representatives. Generating these reference genomes of uncultured microbes will dramatically increase the discovery rate of novel protein families and biological functions, shed light on the numerous underrepresented phyla that likely play important roles in the environment, and will assist in improving the reconstruction of the evolutionary history of Bacteria and Archaea. Moreover, these data will improve our ability to interpret metagenomics sequence data from diverse environments, which will be of tremendous value for microbial ecology and evolutionary studies to come.

  10. Expansion of the Genomic Encyclopedia of Bacteria and Archaea

    SciTech Connect (OSTI)

    Rinke, Christian; Sczyrba, Alex; Malfatti, Stephanie; Lee, Janey; Cheng, Jan-Fang; Stepanauskas, Ramunas; Eisen, Jonathan A.; Hallam, Steven; Inskeep, William P.; Hedlund, Brian P.; Sievert, Stefan M.; Liu, Wen-Tso; Tsiamis, George; Hugenholtz, Philip; Woyke, Tanja

    2011-06-02

    To date the vast majority of bacterial and archaeal genomes sequenced are of rather limited phylogenetic diversity as they were chosen based on their physiology and/ or medical importance. The Genomic Encyclopedia of Bacteria and Archaea (GEBA) project (Wu et al. 2009) is aimed at systematically filling the gaps of the tree of life with phylogenetically diverse reference genomes. However more than 99 percent of microorganisms elude current culturing attempts, severely limiting the ability to recover complete or even partial genomes of these largely mysterious species. These limitations gave rise to the GEBA uncultured project. Here we propose to use single cell genomics to massively expand the Genomic Encyclopedia of Bacteria and Archaea by targeting 80 single cell representatives of uncultured candidate phyla which have no or very few cultured representatives. Generating these reference genomes of uncultured microbes will dramatically increase the discovery rate of novel protein families and biological functions, shed light on the numerous underrepresented phyla that likely play important roles in the environment, and will assist in improving the reconstruction of the evolutionary history of Bacteria and Archaea. Moreover, these data will improve our ability to interpret metagenomics sequence data from diverse environments, which will be of tremendous value for microbial ecology and evolutionary studies to come.

  11. Field application of a genetically engineered microorganism for polycyclic aromatic hydrocarbon bioremediation process monitoring and control

    SciTech Connect (OSTI)

    Sayler, G.S.; Cox, C.D.; Ripp, S.; Nivens, D.E.; Werner, C.; Ahn, Y.; Matrubutham, U.; Burlage, R.

    1998-11-01

    On October 30, 1996, the US Environmental Protection Agency (EPA) commenced the first test release of genetically engineered microorganisms (GEMs) for use in bioremediation. The specific objectives of the investigation were multifaceted and include (1) testing the hypothesis that a GEM can be successfully introduced and maintained in a bioremediation process, (2) testing the concept of using, at the field scale, reporter organisms for direct bioremediation process monitoring and control, and (3) acquiring data that can be used in risk assessment decision making and protocol development for future field release applications of GEMs. The genetically engineered strain under investigation is Pseudomonas fluorescens strain HK44 (King et al., 1990). The original P. fluorescens parent strain was isolated from polycyclic aromatic hydrocarbon (PAH) contaminated manufactured gas plant soil. Thus, this bacterium is able to biodegrade naphthalene (as well as other substituted naphthalenes and other PAHs) and is able to function as a living bioluminescent reporter for the presence of naphthalene contamination, its bioavailability, and the functional process of biodegradation. A unique component of this field investigation was the availability of an array of large subsurface soil lysimeters. This article describes the experience associated with the release of a genetically modified microorganism, the lysimeter facility and its associated instrumentation, as well as representative data collected during the first eighteen months of operation.

  12. Magnetic Microstructure of Magnetotactic Bacteria by

    E-Print Network [OSTI]

    Dunin-Borkowski, Rafal E.

    Magnetic Microstructure of Magnetotactic Bacteria by Electron Holography Rafal E. Dunin microstructure of magnetite nanocrys- tals in magnetotactic bacteria. The magnetite crystals were all single). For example, magnetotactic bacteria contain magnetosomes, which are intracellular, ferri- magnetic crystals

  13. Deciphering Active Estrogen-Degrading Microorganisms in Bioreactors 

    E-Print Network [OSTI]

    Roh, Hyung Keun

    2010-10-12

    degraders (strains KC8 and ammonia-oxidizing bacteria (AOB)) and amoA gene (associated with ammonia oxidation) to total bacteria decreased as SRT increased in SBRs. These observations correspond to the decreasing percentages of 17 beta-estradiol biodegraded...

  14. TSSWCB Bacteria-Related Projects 

    E-Print Network [OSTI]

    Wythe, Kathy

    2007-01-01

    stream_source_info TSSWCB bacteria-related projects.pdf.txt stream_content_type text/plain stream_size 2550 Content-Encoding ISO-8859-1 stream_name TSSWCB bacteria-related projects.pdf.txt Content-Type text/plain; charset=ISO-8859... of the projects are listed below. ? Peach CreekWater Quality Improvement Project ? Monitoring and Educational Programs Focused on Bacteria and Nutrient Runoff on Dairy Operations in the LeonWatershed ? Development of the Plum CreekWPP ? Impact of Proper...

  15. Liquid Fuel from Heat-Loving Microorganisms: H2-Dependent Conversion of CO2 to Liquid Electrofuels by Extremely Thermophilic Archaea

    SciTech Connect (OSTI)

    None

    2010-07-01

    Electrofuels Project: NC State is working with the University of Georgia to create Electrofuels from primitive organisms called extremophiles that evolved before photosynthetic organisms and live in extreme, hot water environments with temperatures ranging from 167-212 degrees Fahrenheit The team is genetically engineering these microorganisms so they can use hydrogen to turn carbon dioxide directly into alcohol-based fuels. High temperatures are required to distill the biofuels from the water where the organisms live, but the heat-tolerant organisms will continue to thrive even as the biofuels are being distilled—making the fuel-production process more efficient. The microorganisms don’t require light, so they can be grown anywhere—inside a dark reactor or even in an underground facility.

  16. Geobiology of marine magnetotactic bacteria

    E-Print Network [OSTI]

    Simmons, Sheri Lynn

    2006-01-01

    Magnetotactic bacteria (MTB) biomineralize intracellular membrane-bound crystals of magnetite (Fe3O4) or greigite (Fe3S4), and are abundant in the suboxic to anoxic zones of stratified marine environments worldwide. Their ...

  17. In Situ Survival of Genetically Engineered Microorganisms in a Tropical

    E-Print Network [OSTI]

    Hazen, Terry

    . Alvarez, G. M. Yumet, and C. L. Santiago Department of Biology, P.O. Box 23360, University of Puerto Rico, Rio Piedras, Puerto Rico 00931-3360 T. C. Hazen Savannah River Laboratory, Environmental Sciences microorganisms (GEMs) and their interactions with the environmental microbiota of a tropical river

  18. Early Detection Saves Lives

    Office of Energy Efficiency and Renewable Energy (EERE)

    Former Department of Energy (DOE) workers tell how medical screening helped them lead healthier and longer lives.

  19. FUTURE LOGISTICS LIVING LABORATORY

    E-Print Network [OSTI]

    Heiser, Gernot

    FUTURE LOGISTICS LIVING LABORATORY Delivering Innovation The Future Logistics Living Lab that will provide logistics solutions for the future. The Living Lab is a demonstration, exhibition and work space by a group of logistics companies, research organisations, universities, and IT providers that includes NICTA

  20. Dynamic Metabolism Studies of Live Bacterial Films

    SciTech Connect (OSTI)

    Majors, Paul D.; Mclean, Jeffrey S.

    2008-11-01

    Bacterial film (biofilm) microbes exist within spatial (nutrient, electron-acceptor, pH, etc.) gradients of their own making. Correspondingly, biofilm bacteria are physiologically and functionally distinct from free-floating bacteria and from their own species at differing biofilm depths. This article describes our efforts to develop noninvasive nuclear magnetic resonance (NMR) technologies for biofilm-metabolism studies. This involves integrating NMR with controlled-cultivation methods to interrogate microbial physiology live and under known growth conditions. NMR is uniquely capable of providing depth-resolved metabolic and transport information in a non-invasive, non-sample-consuming fashion, providing information required for experimental reactive transport studies. We have studied mono-species biofilms relevant to environment remediation and human health. We describe these technologies, discuss their advantages and limitations, and give examples of their application.

  1. Studies of unicellular micro-organisms Saccharomyces cerevisiae by means of Positron Annihilation Lifetime Spectroscopy

    E-Print Network [OSTI]

    Kubicz, E; Zgardzi?ska, B; Bednarski, T; Bia?as, P; Czerwi?ski, E; Gajos, A; Gorgol, M; Kami?ska, D; Kap?on, ?; Kochanowski, A; Korcyl, G; Kowalski, P; Kozik, T; Krzemie?, W; Nied?wiecki, S; Pa?ka, M; Raczy?ski, L; Rajfur, Z; Rudy, Z; Rundel, O; Sharma, N G; Silarski, M; S?omski, A; Strzelecki, A; Wieczorek, A; Wi?licki, W; Zieli?ski, M; Moskal, P

    2015-01-01

    Results of Positron Annihilation Lifetime Spectroscopy (PALS) and microscopic studies on simple microorganisms: brewing yeasts are presented. Lifetime of ortho - positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer lived component) for lyophilised and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water - the main component of the cell - affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilised, dried and wet yeasts with best possible resolution were obtained using Inverted Microscopy (IM) and Environmental Scanning Electron Microscopy (ESEM) methods. As a result visible changes to the surface of the cell membrane were observed in ESEM images.

  2. Aerobic microorganism for the degradation of chlorinated aliphatic hydrocarbons

    DOE Patents [OSTI]

    Fliermans, Carl B. (Augusta, GA)

    1989-01-01

    A chlorinated aliphatic hydrocarbon-degrading microorganism, having American Type Culture Collection accession numbers ATCC 53570 and 53571, in a biologically pure culture aseptically collected from a deep subsurface habitat and enhanced, mineralizes trichloroethylene and tetrachloroethylene to HCl, H.sub.2 O and Co.sub.2 under aerobic conditions stimulated by methane, acetate, methanol, tryptone-yeast extract, propane and propane-methane.

  3. Use of Stable Isotopes in Forensic Analysis of Microorganisms

    SciTech Connect (OSTI)

    Kreuzer-Martin, Helen W.; Hegg, Eric L.

    2012-01-18

    The use of isotopic signatures for forensic analysis of biological materials is well-established, and the same general principles that apply to interpretation of stable isotope content of C, N, O, and H apply to the analysis of microorganisms. Heterotrophic microorganisms derive their isotopic content from their growth substrates, which are largely plant and animal products, and the water in their culture medium. Thus the isotope signatures of microbes are tied to their growth environment. The C, N, O, and H isotope ratios of spores have been demonstrated to constitute highly discriminating signatures for sample matching. They can rule out specific samples of media and/or water as possible production media, and can predict isotope ratio ranges of the culture media and water used to produce a given sample. These applications have been developed and tested through analyses of approximately 250 samples of Bacillus subtilis spores and over 500 samples of culture media, providing a strong statistical basis for data interpretation. A Bayesian statistical framework for integrating stable isotope data with other types of signatures derived from microorganisms has been able to characterize the culture medium used to produce spores of various Bacillus species, leveraging isotopic differences in different medium types and demonstrating the power of data integration for forensic investigations.

  4. ACCOUNTING ROADMAP TRANSFORMING LIVES

    E-Print Network [OSTI]

    ACCOUNTING ROADMAP TO SUCCESS THE TRANSFORMING LIVES COLLEGE OF BUSINESS Department of Accounting #12;TABLEOFCONTENTS Greetings from Accounting Department Chair ..............................2 What is Accounting? .......................................................................4 Successful Study

  5. How do microbial fuel cells (MFCs) work? Bacteria need energy to survive, in the same way that humans need food to

    E-Print Network [OSTI]

    Lee, Dongwon

    How do microbial fuel cells (MFCs) work? Bacteria need energy to survive, in the same way that humans need food to live. Bacteria get this energy in a two-step process. The first step requires are receiving more attention because they are a potential part of the solution to our energy demands and could

  6. Structural conservation of chemotaxis machinery across Archaea and Bacteria

    E-Print Network [OSTI]

    Briegel, A; Ortega, DR; Huang, AN; Oikonomou, CM; Gunsalus, RP; Jensen, GJ

    2015-01-01

    J. (1966) Chemotaxis in Bacteria. Science 153: 708-716.gene transfer between bacteria and archaea. J Mol MicrobiolProtein Localization in Bacteria. J Bacteriol 183: 3261-

  7. High-throughput comparison of gene fitness among related bacteria

    E-Print Network [OSTI]

    2012-01-01

    5096–5108. 36. Macnab RM: How bacteria assemble. Annu Revgene fitness among related bacteria. BMC Genomics 2012 13:gene fitness among related bacteria Rocio Canals 1 , Xiao-

  8. Expression of Genes Linked to NOx Detoxification in Aerobic Bacteria

    E-Print Network [OSTI]

    Cua, Lynnie

    2010-01-01

    ecology of nitrifying bacteria. In: Biology of the Nitrogennitrogen metabolism in bacteria. Current Opinion in Chemicalin ammonia-oxidizing bacteria. Incomplete oxidation of NH 2

  9. Bacteria Associated With Colitis and Inflammatory Bowel Disease

    E-Print Network [OSTI]

    Ye, Jingxiao

    2009-01-01

    M. and J. M. Rhodes (2000). "Bacteria and inflammatory boweltwo different commensal bacteria." Gastroenterology 128(4):M. and J. M. Rhodes (2000). "Bacteria and inflammatory bowel

  10. Revisiting Modes of energy generation in sulfate reducing bacteria

    E-Print Network [OSTI]

    Joachimiak, Marcin

    2010-01-01

    generation in sulfate reducing bacteria 1. Lawrence BerkeleyAbstract Sulfate reducing bacteria (SRB) play an importantin the sulfate-reducing bacteria Desulfovibrio sp." FEMS

  11. Genomic analysis of high pressure adaptation in deep sea bacteria

    E-Print Network [OSTI]

    Stratton, Taylor Kristen

    2008-01-01

    of proteins in halophilic bacteria. J Mol Bacteriol. 327:Phylogenetic analysis of bacteria preserved in a permafrostof piezophilic bacteria isolated from intestinal contents of

  12. Re-engineering bacteria for ethanol production

    DOE Patents [OSTI]

    Yomano, Lorraine P; York, Sean W; Zhou, Shengde; Shanmugam, Keelnatham; Ingram, Lonnie O

    2014-05-06

    The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.

  13. Microorganisms to Speed Production of Biofuels - Energy Innovation Portal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration wouldMass map shines light on darkMicroorganisms to Speed Production of Biofuels Oak Ridge

  14. RESEARCH ARTICLE Functional Characterization of Bacteria

    E-Print Network [OSTI]

    Desai, Michael

    resistance mechanisms at least 5,000 years ago. Among bacteria sampled from the ancient layers bacteria conferred lower levels of resis- tance against clinically relevant antibiotics than resistance) Functional Characterization of Bacteria Isolated from Ancient Arctic Soil Exposes Diverse Resistance

  15. Drosophila lifespan enhancement by exogenous bacteria

    E-Print Network [OSTI]

    Seroude, Laurent

    Drosophila lifespan enhancement by exogenous bacteria Ted Brummel*, Alisa Ching*, Laurent Seroude with customary procedure. The experiments revealed that the presence of bacteria during the first week of adult life can enhance lifespan, despite unchanged food intake. Later in life, the presence of bacteria can

  16. MicroReview Bistability in bacteria

    E-Print Network [OSTI]

    Mekalanos, John

    MicroReview Bistability in bacteria David Dubnau1 * and Richard Losick2,3 * 1 Public Health 94720, USA. Summary Gene expression in bacteria is traditionally studied from the average behaviour expression in individual cells reveals, however, that populations of genetically identical bacteria

  17. Mechanism of Transcriptional Bursting in Bacteria

    E-Print Network [OSTI]

    Li, Tiejun

    Mechanism of Transcriptional Bursting in Bacteria Shasha Chong,1,5 Chongyi Chen,1,2,5 Hao Ge,3 phenomenon has not been understood. Here, we present the mechanism in bacteria. We developed a high. Together, these findings prove that transcriptional bursting of highly ex- pressed genes in bacteria

  18. Metagenomic and Cultivation-Based Analysis of Novel Microorganisms and Functions in Metal-Contaminated Environments

    E-Print Network [OSTI]

    Yelton, Alexis Pepper

    2012-01-01

    of copper resistance and accumulation in bacteria. FEMSresistance in addition to energy conservation. Here we report the isolation of a bacteria

  19. Effects of remediation amendments on vadose zone microorganisms

    SciTech Connect (OSTI)

    Miller, Hannah M.; Tilton, Fred A.

    2012-08-10

    Surfactant-based foam delivery technology has been studied to remediate Hanford 200 area deep vadose zone sediment. However, the surfactants and remediation amendments have an unknown effect on indigenous subsurface microorganisms. Microbial populations are important factors to consider in remediation efforts due to their potential to alter soil geochemistry. This project focuses on measuring microbial metabolic responses to remediation amendments in batch and column studies using Deep Vadose Zone Sediments. Initial studies of the microbes from Hanford 200 area deep vadose zone sediment showed surfactants sodium dodecyl sulfate (SDS) and cocamidopropyl betaine (CAPB) and remediation amendment calcium polysulfide (CPS) had no affect on microbial growth using BiologTM Ecoplates. To move towards a more realistic field analog, soil columns were packed with Hanford 200 Area sediment. Once microbial growth in the column was verified by observing growth of the effluent solution on tryptic soy agar plates, remedial surfactants were injected into the columns, and the resulting metabolic diversity was measured. Results suggest surfactant sodium dodecyl sulfate (SDS) stimulates microbial growth. The soil columns were also visualized using X-ray microtomography to inspect soil packing and possibly probe for evidence of biofilms. Overall, BiologTM Ecoplates provide a rapid assay to predict effects of remediation amendments on Hanford 200 area deep vadose zone microorganisms.

  20. Protein signaling via type III secretion pathways in phytopathogenic bacteria

    E-Print Network [OSTI]

    109 Protein signaling via type III secretion pathways in phytopathogenic bacteria Mary Beth Mudgett secretion pathway has revealed new mechanisms by which phytopathogenic bacteria infect plants are continually exposed to a number of potentially pathogenic bacteria. Phytopathogenic bacteria, in general

  1. Correlative Electron and Fluorescence Microscopy of Magnetotactic Bacteria in Liquid: Toward In Vivo Imaging

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Woehl, Taylor J.; Kashyap, Sanjay; Firlar, Emre; Perez-Gonzalez, Teresa; Faivre, Damien; Trubitsyn, Denis; Bazylinski, Dennis A.; Prozorov, Tanya

    2014-10-31

    Magnetotactic bacteria biomineralize ordered chains of uniform, membrane-bound magnetite or greigite nanocrystals that exhibit nearly perfect crystal structures and species-specific morphologies. Transmission electron microscopy (TEM) is a critical technique for providing information regarding the organization of cellular and magnetite structures in these microorganisms. However, conventional TEM can only be used to image air-dried or vitrified bacteria removed from their natural environment. Here we present a correlative scanning TEM (STEM) and fluorescence microscopy technique for imaging viable cells of Magnetospirillum magneticum strain AMB-1 in liquid using an in situ fluid cell TEM holder. Fluorescently labeled cells were immobilized on microchip windowmore »surfaces and visualized in a fluid cell with STEM, followed by correlative fluorescence imaging to verify their membrane integrity. Notably, the post-STEM fluorescence imaging indicated that the bacterial cell wall membrane did not sustain radiation damage during STEM imaging at low electron dose conditions. We investigated the effects of radiation damage and sample preparation on the bacteria viability and found that approximately 50% of the bacterial membranes remained intact after an hour in the fluid cell, decreasing to ~30% after two hours. These results represent a first step toward in vivo studies of magnetite biomineralization in magnetotactic bacteria.« less

  2. Correlative Electron and Fluorescence Microscopy of Magnetotactic Bacteria in Liquid: Toward In Vivo Imaging

    SciTech Connect (OSTI)

    Woehl, Taylor J.; Kashyap, Sanjay; Firlar, Emre; Perez-Gonzalez, Teresa; Faivre, Damien; Trubitsyn, Denis; Bazylinski, Dennis A.; Prozorov, Tanya

    2014-10-31

    Magnetotactic bacteria biomineralize ordered chains of uniform, membrane-bound magnetite or greigite nanocrystals that exhibit nearly perfect crystal structures and species-specific morphologies. Transmission electron microscopy (TEM) is a critical technique for providing information regarding the organization of cellular and magnetite structures in these microorganisms. However, conventional TEM can only be used to image air-dried or vitrified bacteria removed from their natural environment. Here we present a correlative scanning TEM (STEM) and fluorescence microscopy technique for imaging viable cells of Magnetospirillum magneticum strain AMB-1 in liquid using an in situ fluid cell TEM holder. Fluorescently labeled cells were immobilized on microchip window surfaces and visualized in a fluid cell with STEM, followed by correlative fluorescence imaging to verify their membrane integrity. Notably, the post-STEM fluorescence imaging indicated that the bacterial cell wall membrane did not sustain radiation damage during STEM imaging at low electron dose conditions. We investigated the effects of radiation damage and sample preparation on the bacteria viability and found that approximately 50% of the bacterial membranes remained intact after an hour in the fluid cell, decreasing to ~30% after two hours. These results represent a first step toward in vivo studies of magnetite biomineralization in magnetotactic bacteria.

  3. Organic acid-tolerant microorganisms and uses thereof for producing organic acids

    DOE Patents [OSTI]

    Pfleger, Brian Frederick; Begemann, Matthew Brett

    2014-05-06

    Organic acid-tolerant microorganisms and methods of using same. The organic acid-tolerant microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid (3HP), acrylic acid, and propionic acid. Further modifications to the microorganisms such as increasing expression of malonyl-CoA reductase and/or acetyl-CoA carboxylase provide or increase the ability of the microorganisms to produce 3HP. Methods of generating an organic acid with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers include replacing acsA or homologs thereof in cells with genes of interest and selecting for the cells comprising the genes of interest with amounts of organic acids effective to inhibit growth of cells harboring acsA or the homologs.

  4. Methods for identifying an essential gene in a prokaryotic microorganism

    DOE Patents [OSTI]

    Shizuya, Hiroaki

    2006-01-31

    Methods are provided for the rapid identification of essential or conditionally essential DNA segments in any species of haploid cell (one copy chromosome per cell) that is capable of being transformed by artificial means and is capable of undergoing DNA recombination. This system offers an enhanced means of identifying essential function genes in diploid pathogens, such as gram-negative and gram-positive bacteria.

  5. Sensory Transduction in Microorganisms 2008 Gordon Research Conference (January 2008)

    SciTech Connect (OSTI)

    Ann M. Stock

    2009-04-08

    Research into the mechanisms involved in the sensing and responses of microorganisms to changes in their environments is currently very active in a large number of laboratories worldwide. An increasingly wide range of prokaryotic and eukaryotic species are being studied with regard to their sensing of diverse chemical and physical stimuli, including nutrients, toxins, intercellular signaling molecules, redox indicators, light, pressure, magnetic fields, and surface contact, leading to adaptive responses affecting motile behavior, gene expression and/or development. The ease of manipulation of microorganisms has facilitated application of a broad range of techniques that have provided comprehensive descriptions of cellular behavior and its underlying molecular mechanisms. Systems and their molecular components have been probed at levels ranging from the whole organism down to atomic resolution using behavioral analyses; electrophysiology; genetics; molecular biology; biochemical and biophysical characterization; structural biology; single molecule, fluorescence and cryo-electron microscopy; computational modeling; bioinformatics and genomic analyses. Several model systems such as bacterial chemotaxis and motility, fruiting body formation in Myxococcus xanthus, and motility and development in Dictyostelium discoideum have traditionally been a focus of this meeting. By providing a basis for assessment of similarities and differences in mechanisms, understanding of these pathways has advanced the study of many other microbial sensing systems. This conference aims to bring together researchers investigating different prokaryotic and eukaryotic microbial systems using diverse approaches to compare data, share methodologies and ideas, and seek to understand the fundamental principles underlying sensory responses. Topic areas include: (1) Receptor Sensing and Signaling; (2) Intracellular Signaling (two-component, c-di-GMP, c-AMP, etc.); (3) Intracellular Localization and the Cytoskeleton; (4) Motors and Motility; (5) Differentiation and Development; (6) Host/Pathogen and Host/Symbiont Interactions; (7) Intercellular Communication; (8) Microbes and the Environment; and (9) Modeling Signaling Pathways.

  6. FROM MATHEMATICS FOR LIVING TO LIVING FOR MATHEMATICS

    E-Print Network [OSTI]

    Spagnolo, Filippo

    FROM MATHEMATICS FOR LIVING TO LIVING FOR MATHEMATICS George Malaty, University of Joensuu, Finland "Life is good for only two things, discovering mathematics and teaching mathematics". Siméon Poisson (1781-1840) Mathematics for living and living for mathematics are related to the goals of mathematics

  7. Transformation of gram positive bacteria by sonoporation

    DOE Patents [OSTI]

    Yang, Yunfeng; Li, Yongchao

    2014-03-11

    The present invention provides a sonoporation-based method that can be universally applied for delivery of compounds into Gram positive bacteria. Gram positive bacteria which can be transformed by sonoporation include, for example, Bacillus, Streptococcus, Acetobacterium, and Clostridium. Compounds which can be delivered into Gram positive bacteria via sonoporation include nucleic acids (DNA or RNA), proteins, lipids, carbohydrates, viruses, small organic and inorganic molecules, and nano-particles.

  8. Managing Bacteria Pollution in Texas Waters 

    E-Print Network [OSTI]

    Wythe, Kathy

    2007-01-01

    Wythe tx H2O | pg. 2 BACTERIA MANAGING tx H2O | pg. 3 IN TEXAS WATERS POLLUTION Managing Bacteria Pollution in Texas Waters tx H2O | pg. 4 W ith 310 water bodies in Texas failing to meetwater quality standards because of bacteria,managing bacteria... pollution is commanding the attention of water agencies, researchers and stake- holders across Texas. These water bodies are listed in the 2006 Texas Water Quality Inventory and 303(d) List for failing to meet the standards designed to protect...

  9. Spectroscopic diagnostics for bacteria in biologic sample

    DOE Patents [OSTI]

    El-Sayed, Mostafa A. (Atlanta, GA); El-Sayed, Ivan H. (Somerville, MA)

    2002-01-01

    A method to analyze and diagnose specific bacteria in a biologic sample using spectroscopy is disclosed. The method includes obtaining the spectra of a biologic sample of a non-infected patient for use as a reference, subtracting the reference from the spectra of an infected sample, and comparing the fingerprint regions of the resulting differential spectrum with reference spectra of bacteria in saline. Using this diagnostic technique, specific bacteria can be identified sooner and without culturing, bacteria-specific antibiotics can be prescribed sooner, resulting in decreased likelihood of antibiotic resistance and an overall reduction of medical costs.

  10. Engineering and Coordination of Regulatory Networks and Intracellular Complexes to Maximize Hydrogen Production by Phototrophic Microorganisms

    SciTech Connect (OSTI)

    James C. Liao

    2012-05-22

    This project is a collaboration with F. R. Tabita of Ohio State. Our major goal is to understand the factors and regulatory mechanisms that influence hydrogen production. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Our part of the project was to develop a modeling technique to investigate the metabolic network in connection to hydrogen production and regulation. Organisms must balance the pathways that generate and consume reducing power in order to maintain redox homeostasis to achieve growth. Maintaining this homeostasis in the nonsulfur purple photosynthetic bacteria is a complex feat with many avenues that can lead to balance, as these organisms possess versatile metabolic capabilities including anoxygenic photosynthesis, aerobic or anaerobic respiration, and fermentation. Growth is achieved by using H{sub 2} as an electron donor and CO{sub 2} as a carbon source during photoautotrophic and chemoautotrophic growth, where CO{sub 2} is fixed via the Calvin-Benson-Bassham (CBB) cycle. Photoheterotrophic growth can also occur when alternative organic carbon compounds are utilized as both the carbon source and electron donor. Regardless of the growth mode, excess reducing equivalents generated as a result of oxidative processes, must be transferred to terminal electron acceptors, thus insuring that redox homeostasis is maintained in the cell. Possible terminal acceptors include O{sub 2}, CO{sub 2}, organic carbon, or various oxyanions. Cells possess regulatory mechanisms to balance the activity of the pathways which supply energy, such as photosynthesis, and those that consume energy, such as CO{sub 2} assimilation or N{sub 2} fixation. The major route for CO{sub 2} assimilation is the CBB reductive pentose phosphate pathway, whose key enzyme is ribulose 1,5-biphosphate carboxylase/oxygenase (RubisCO). In addition to providing virtually all cellular carbon during autotrophic metabolism, RubisCO-mediated CO{sub 2} assimilation is also very important for nonsulfur purple photosynthetic bacteria under photoheterotrophic growth conditions since CO{sub 2} becomes the major electron sink under these conditions. In this work, Ensemble Modeling (EM) was developed to examine the behavior of CBB-compromised RubisCO knockout mutant strains of the nonsulfur purple photosynthetic bacterium Rhodobacter sphaeroides. Mathematical models of metabolism can be a great aid in studying the effects of large perturbations to the system, such as the inactivation of RubisCO. Due to the complex and highly-interconnected nature of these networks, it is not a trivial process to understand what the effect of perturbations to the metabolic network will be, or vice versa, what enzymatic perturbations are necessary to yield a desired effect. Flux distribution is controlled by multiple enzymes in the network, often indirectly linked to the pathways of interest. Further, depending on the state of the cell and the environmental conditions, the effect of a perturbation may center around how it effects the carbon flow in the network, the balancing of cofactors, or both. Thus, it is desirable to develop mathematical models to describe, understand, and predict network behavior. Through the development of such models, one may gain the ability to generate a set of testable hypotheses for system behavior.

  11. Apparatus and method for transforming living cells

    DOE Patents [OSTI]

    Okandan, Murat; Galambos, Paul C.

    2003-11-11

    An apparatus and method are disclosed for in vitro transformation of living cells. The apparatus, which is formed as a microelectromechanical device by surface micromachining, can be used to temporarily disrupt the cell walls or membrane of host cells one at a time so that a particular substance (e.g. a molecular tag, nucleic acid, bacteria, virus etc.) can be introduced into the cell. Disruption of the integrity of the host cells (i.e. poration) can be performed mechanically or electrically, or by both while the host cells are contained within a flow channel. Mechanical poration is possible using a moveable member which has a pointed or serrated edge and which is driven by an electrostatic actuator to abrade, impact or penetrate the host cell. Electroporation is produced by generating a relatively high electric field across the host cell when the host cell is located in the flow channel between a pair of electrodes having a voltage applied therebetween.

  12. ACCOUNTING ROADMAP TRANSFORMING LIVES

    E-Print Network [OSTI]

    Collett Jr., Jeffrey L.

    ACCOUNTING ROADMAP TO SUCCESS THE TRANSFORMING LIVES COLLEGE OF BUSINESS Department of Accounting #12;TABLEOFCONTENTS Greetings from Accounting Department Chair 2 What is Accounting? 4 Successful Accounting Career Paths 8 Careers in Managerial Accounting 9 Careers in Government/Not-for-Profit (GNP

  13. RESEARCH ARTICLE Adaptive Resistance in Bacteria Requires

    E-Print Network [OSTI]

    Cluzel, Philippe

    RESEARCH ARTICLE Adaptive Resistance in Bacteria Requires Epigenetic Inheritance, Genetic Noise Resistance in Bacteria Requires Epigenetic Inheritance, Genetic Noise, and Cost of Efflux Pumps. PLoS ONE 10, Cambridge, Massachusetts, United States of America * max@fis.unam.mx Abstract Adaptive resistance emerges

  14. Method of dispersing a hydrocarbon using bacteria

    DOE Patents [OSTI]

    Tyndall, Richard L. (Clinton, TN)

    1996-01-01

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  15. Method of dispersing a hydrocarbon using bacteria

    DOE Patents [OSTI]

    Tyndall, R.L.

    1996-09-24

    A new protozoan derived microbial consortia and method for their isolation are provided. The isolated consortia and bacteria are useful for treating wastes such as trichloroethylene and trinitrotoluene. The isolated consortia, bacteria, and dispersants are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  16. Chemotactic selection of pollutant degrading soil bacteria

    DOE Patents [OSTI]

    Hazen, T.C.

    1991-03-04

    A method is described for identifying soil microbial strains which may be bacterial degraders of pollutants. This method includes: Placing a concentration of a pollutant in a substantially closed container; placing the container in a sample of soil for a period of time ranging from one minute to several hours; retrieving the container and collecting its contents; microscopically determining the identity of the bacteria present. Different concentrations of the pollutant can be used to determine which bacteria respond to each concentration. The method can be used for characterizing a polluted site or for looking for naturally occurring biological degraders of the pollutant. Then bacteria identified as degraders of the pollutant and as chemotactically attracted to the pollutant are used to innoculate contaminated soil. To enhance the effect of the bacteria on the pollutant, nutrients are cyclicly provided to the bacteria then withheld to alternately build up the size of the bacterial colony or community and then allow it to degrade the pollutant.

  17. Final Technical Report: Viral Infection of Subsurface Microorganisms and Metal/Radionuclide Transport

    SciTech Connect (OSTI)

    Weber, Karrie A.; Bender, Kelly S.; Li, Yusong

    2013-09-28

    Microbially mediated metabolisms have been identified as a significant factor either directly or indirectly impacting the fate and transport of heavy metal/radionuclide contaminants. To date microorganisms have been isolated from contaminated environments. Examination of annotated finished genome sequences of many of these subsurface isolates from DOE sites, revealed evidence of prior viral infection. To date the role that viruses play influencing microbial mortality and the resulting community structure which directly influences biogeochemical cycling in soils and sedimentary environments remains poorly understood. The objective of this exploratory study was to investigate the role of viral infection of subsurface bacteria and the formation of contaminant-bearing viral particles. This objective was approached by examining the following working hypotheses: (i) subsurface microorganisms are susceptible to viral infections by the indigenous subsurface viral community, and (ii) viral surfaces will adsorb heavy metals and radionuclides. Our results have addressed basic research needed to accomplish the BER Long Term Measure to provide sufficient scientific understanding such that DOE sites would be able to incorporate coupled physical, chemical and biological processes into decision making for environmental remediation or natural attenuation and long-term stewardship by establishing viral-microbial relationships on the subsequent fate and transport of heavy metals and radionuclides. Here we demonstrated that viruses play a significant role in microbial mortality and community structure in terrestrial subsurface sedimentary systems. The production of viral-like particles within subsurface sediments in response to biostimulation with dissolved organic carbon and a terminal electron acceptor resulted in the production of viral-like particles. Organic carbon alone did not result in significant viral production and required the addition of a terminal electron acceptor (nitrate), indicating that nutrients are not limiting viral production, but rather substrates that can be converted into energy for host metabolism. Our results also revealed that cell abundance was not correlated to the mineralization of organic carbon, but rather viruses were positively correlated with carbon mineralization. This is a result of viral-mediated cell lysis and demonstrates that viruses are sensitive indicators of microbial activity. Viruses as an indicator of microbial activity was not unique to batch culture studies as results obtained from an in situ field experiment conducted at the DOE Old Rifle Field site. This study revealed that viral abundance increased in response to the injection of oxygenated groundwater and influx of dissolved organic carbon whereas cell abundance changes were minimal. However, the extent to which viral-mediated cell lysis alters organic matter pools subsequently influencing microbial community structure and biogeochemical function remains a critical question in subsurface biogeochemical cycling. The production of significant numbers of viruses in groundwater has implications for nanoparticulate metal as well as carbon transport in groundwater. We have demonstrated that the virus surface is reactive and will adsorb heavy metals. Thus viruses can promote colloidal contaminant mobility. Interestingly, the presence of heavy metals has a positive effect on infectivity of the phage, increasing phage infection which could lead to further production of viruses. Together, the results indicate that the sorption of metals to the surface of viruses could not only contribute to nanoparticulate metal as well as carbon transport but could also enhance infectivity further contributing to cell lysis which could subsequently influence biogeochemical cycling. As more viruses infect host microbial populations the high concentration of metals would enhance infection, resulting in cell lysis, and decreasing the metabolically active host population while yielding greater numbers of viruses capable of transporting contaminats. Additional studie

  18. Metabolic Engineering and Modeling of Metabolic Pathways to Improve Hydrogen Production by Photosynthetic Bacteria

    SciTech Connect (OSTI)

    Jiao, Y.; Navid, A.

    2014-12-19

    Rising energy demands and the imperative to reduce carbon dioxide (CO2) emissions are driving research on biofuels development. Hydrogen gas (H2) is one of the most promising biofuels and is seen as a future energy carrier by virtue of the fact that 1) it is renewable, 2) does not evolve the “greenhouse gas” CO2 in combustion, 3) liberates large amounts of energy per unit weight in combustion (having about 3 times the energy content of gasoline), and 4) is easily converted to electricity by fuel cells. Among the various bioenergy strategies, environmental groups and others say that the concept of the direct manufacture of alternative fuels, such as H2, by photosynthetic organisms is the only biofuel alternative without significant negative criticism [1]. Biological H2 production by photosynthetic microorganisms requires the use of a simple solar reactor such as a transparent closed box, with low energy requirements, and is considered as an attractive system to develop as a biocatalyst for H2 production [2]. Various purple bacteria including Rhodopseudomonas palustris, can utilize organic substrates as electron donors to produce H2 at the expense of solar energy. Because of the elimination of energy cost used for H2O oxidation and the prevention of the production of O2 that inhibits the H2-producing enzymes, the efficiency of light energy conversion to H2 by anoxygenic photosynthetic bacteria is in principle much higher than that by green algae or cyanobacteria, and is regarded as one of the most promising cultures for biological H2 production [3]. Here implemented a simple and relatively straightforward strategy for hydrogen production by photosynthetic microorganisms using sunlight, sulfur- or iron-based inorganic substrates, and CO2 as the feedstock. Carefully selected microorganisms with bioengineered beneficial traits act as the biocatalysts of the process designed to both enhance the system efficiency of CO2 fixation and the net hydrogen production rate. Additionally we applied metabolic engineering approaches guided by computational modeling for the chosen model microorganisms to enable efficient hydrogen production.

  19. Reduction and Immobilization of Radionuclides and Toxic Metal Ions Using Combined Zero Valent Iron and Anaerobic Bacteria

    SciTech Connect (OSTI)

    Lenly J. Weathers; Lynn E. Katz

    2002-05-29

    The use of zero valent iron, permeable reactive barriers (PRBs) for groundwater remediation continues to increase. AN exciting variation of this technology involves introducing anaerobic bacteria into these barriers so that both biological and abiotic pollutant removal processes are functional. This work evaluated the hypothesis that a system combining a mixed culture of sulfate reducing bacteria (SRB) with zero valent iron would have a greater cr(VI) removal efficiency and a greater total Cr(VI) removal capacity than a zero valent iron system without the microorganisms. Hence, the overall goal of this research was to compare the performance of these types of systems with regard to their Cr(VI) removal efficiency and total Cr(VI) removal capacity. Both batch and continuous flow reactor systems were evaluated.

  20. Magnetic Bacteria: A Future in Industry and Medicine

    E-Print Network [OSTI]

    Low, Emily

    2011-01-01

    http://www.eoearth.org/article/Bacteria? topic=49480 http://day be able to use these bacteria and magnetosomes on largeUsing Magnetotactic Bacteria. IEEE Transactions on Magnetics

  1. Bacteria-Phytoplankton Competition Bacterial immobilization or remineralization of N.

    E-Print Network [OSTI]

    Vallino, Joseph J.

    Bacteria-Phytoplankton Competition Overview: · Bacterial immobilization or remineralization of N. · Competition between bacteria and phytoplankton for DIN. · Experimentally examine how dissolved organic carbon (DOC) affects the competition between bacteria and phytoplankton for limiting nutrients. · Demonstrate

  2. Assessing the transport and fate of bioengineered microorganisms in the environment

    SciTech Connect (OSTI)

    Barnthouse, L.W.; Palumbo, A.V.

    1985-01-01

    We review the methods currently available for quantifying the transport and fate of microbes in atmospheric and aqueous media and assess their adequacy for purposes of risk assessment. We review the literature on transport and fate of microorganisms, including studies of: (1) pathways of migration, (2) the survival of microorganisms during transport and fate. In addition, we review the transport and fate models that have been used in environmental risk assessments for radionuclides and toxic chemicals and evaluate their applicability to the problem of assessing environmental risks of bioengineered microorganisms.

  3. Motility fractionation of bacteria by centrifugation

    E-Print Network [OSTI]

    Claudio Maggi; Alessia Lepore; Jacopo Solari; Alessandro Rizzo; Roberto Di Leonardo

    2013-10-10

    Centrifugation is a widespread laboratory technique used to separate mixtures into fractions characterized by a specific size, weight or density. We demonstrate that centrifugation can be also used to separate swimming cells having different motility. To do this we study self-propelled bacteria under the influence of an external centrifugal field. Using dynamic image correlation spectroscopy we measure the spatially resolved motility of bacteria after centrifugation. A significant gradient in swimming-speeds is observed for increasing centrifugal speeds. Our results can be reproduced by a model that treats bacteria as "hot" colloidal particles having a diffusion coefficient that depends on the swimming speed.

  4. Methane and Methanotrophic Bacteria as a Biotechnological Platform...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Methane and Methanotrophic Bacteria as a Biotechnological Platform Methane and Methanotrophic Bacteria as a Biotechnological Platform Breakout Session 2-B: NewEmerging Pathways...

  5. Living olefin polymerization processes

    DOE Patents [OSTI]

    Schrock, R.R.; Baumann, R.

    1999-03-30

    Processes for the living polymerization of olefin monomers with terminal carbon-carbon double bonds are disclosed. The processes employ initiators that include a metal atom and a ligand having two group 15 atoms and a group 16 atom or three group 15 atoms. The ligand is bonded to the metal atom through two anionic or covalent bonds and a dative bond. The initiators are particularly stable under reaction conditions in the absence of olefin monomer. The processes provide polymers having low polydispersities, especially block copolymers having low polydispersities. It is an additional advantage of these processes that, during block copolymer synthesis, a relatively small amount of homopolymer is formed.

  6. Living a Sustainable Future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformationJesse BergkampCenter (LMI-EFRC) ProximityCenterLeeincreases |Living a

  7. Bacteria resist! INRA MIMA2 Imaging Center

    E-Print Network [OSTI]

    Ribot, Magali

    Biofilms: Bacteria resist! #12;INRA MIMA2 Imaging Center #12;#12;Biofilms everywhere ! #12 to biocides #12;MicrocoloniesBiofilm mature Détachement D.Davis, 2007. PLOS àEmergence of resistant mutants

  8. Methods for dispersing hydrocarbons using autoclaved bacteria

    DOE Patents [OSTI]

    Tyndall, Richard L. (Clinton, TN)

    1996-01-01

    A method of dispersing a hydrocarbon includes the steps: providing a bacterium selected from the following group: ATCC 85527, ATCC 75529, and ATCC 55638, a mutant of any one of these bacteria possessing all the identifying characteristics of any one of these bacteria, and mixtures thereof; autoclaving the bacterium to derive a dispersant solution therefrom; and contacting the dispersant solution with a hydrocarbon to disperse the hydrocarbon. Moreover, a method for preparing a dispersant solution includes the following steps: providing a bacterium selected from the following group: ATCC 75527, ATCC 75529, and ATCC 55638, a mutant of any one of these bacteria possessing all the identifying characteristics of any one of these bacteria, and mixtures thereof; and autoclaving the bacterium to derive a dispersant solution therefrom.

  9. Reverse and flick: Hybrid locomotion in bacteria

    E-Print Network [OSTI]

    Stocker, Roman

    Many bacteria are motile. They use one or more helical flagella as propellers, rotating them like the corkscrew on a wine bottle opener. Despite the limited morphological repertoire of the propulsive system, radically ...

  10. Methods for dispersing hydrocarbons using autoclaved bacteria

    DOE Patents [OSTI]

    Tyndall, R.L.

    1996-11-26

    A method of dispersing a hydrocarbon includes the following steps: providing a bacterium selected from the following group: ATCC 85527, ATCC 75529, and ATCC 55638, a mutant of any one of these bacteria possessing all the identifying characteristics of any one of these bacteria, and mixtures; autoclaving the bacterium to derive a dispersant solution; and contacting the dispersant solution with a hydrocarbon to disperse the hydrocarbon. Moreover, a method for preparing a dispersant solution includes the following steps: providing a bacterium selected from the following group: ATCC 75527, ATCC 75529, and ATCC 55638, a mutant of any one of these bacteria possessing all the identifying characteristics of any one of these bacteria, and mixtures; and autoclaving the bacterium to derive a dispersant solution.

  11. How Bacteria Make Magnets | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    How Bacteria Make Magnets For a number of animals, including birds, fish and mammals, there is evidence that magnets are used for orientation. However, little is known about how...

  12. Probiotic Bacteria Induce a ‘Glow of Health’

    E-Print Network [OSTI]

    Levkovich, Tatiana

    Radiant skin and hair are universally recognized as indications of good health. However, this ‘glow of health’ display remains poorly understood. We found that feeding of probiotic bacteria to aged mice induced integumentary ...

  13. Fuel from Bacteria: Bioconversion of Carbon Dioxide to Biofuels by Facultatively Autotrophic Hydrogen Bacteria

    SciTech Connect (OSTI)

    None

    2010-07-01

    Electrofuels Project: Ohio State is genetically modifying bacteria to efficiently convert carbon dioxide directly into butanol, an alcohol that can be used directly as a fuel blend or converted to a hydrocarbon, which closely resembles a gasoline. Bacteria are typically capable of producing a certain amount of butanol before it becomes too toxic for the bacteria to survive. Ohio State is engineering a new strain of the bacteria that could produce up to 50% more butanol before it becomes too toxic for the bacteria to survive. Finding a way to produce more butanol more efficiently would significantly cut down on biofuel production costs and help make butanol cost competitive with gasoline. Ohio State is also engineering large tanks, or bioreactors, to grow the biofuel-producing bacteria in, and they are developing ways to efficiently recover biofuel from the tanks.

  14. Agencies Approve Bacteria TMDL Task Force Recommendations 

    E-Print Network [OSTI]

    Wythe, Kathy

    2007-01-01

    tx H2O | pg. 10 In June 2007 the Texas Commission onEnvironmental Quality (TCEQ) and the TexasState Soil and Water Conservation Board (TSSW- CB) approved the recommendations of the Bacteria Total Maximum Daily Load (TMDL) Task Force and asked... their agencies to update their TMDL guidance documents to reflect these recommendations. They also authorized establishing a multi-agency bacteria TMDL work group to examine the research and development needs identified in the task force report. Both TCEQ...

  15. Nucleic acid molecules conferring enhanced ethanol tolerance and microorganisms having enhanced tolerance to ethanol

    DOE Patents [OSTI]

    Brown, Steven; Guss, Adam; Yang, Shihui; Karpinets, Tatiana; Lynd, Lee; Shao, Xiongjun

    2014-01-14

    The present invention provides isolated nucleic acid molecules which encode a mutant acetaldehyde-CoA/alcohol dehydrogenase or mutant alcohol dehydrogenase and confer enhanced tolerance to ethanol. The invention also provides related expression vectors, genetically engineered microorganisms having enhanced tolerance to ethanol, as well as methods of making and using such genetically modified microorganisms for production of biofuels based on fermentation of biomass materials.

  16. Beating bacteria: Scientists work to understand and track bacteria in water 

    E-Print Network [OSTI]

    Wythe, Kathy

    2010-01-01

    stream_source_info Beating Bacteria.pdf.txt stream_content_type text/plain stream_size 24959 Content-Encoding ISO-8859-1 stream_name Beating Bacteria.pdf.txt Content-Type text/plain; charset=ISO-8859-1 tx H2O | pg. 11... Story by Kathy Wythe Bacteria Task Force Recommendations Acknowledging the enormity of the bacteria problem within the state, in September 2006, TCEQ and the Texas State Soil and Water Conservation Board (TSSWCB) established a joint Task Force...

  17. Analysis of Antibiotic Resistant Genes in Aquacultural Bacteria

    E-Print Network [OSTI]

    Kane, Andrew S.

    1 Analysis of Antibiotic Resistant Genes in Aquacultural Bacteria JY Wang* YJ Geng RX Wang J Feng in the selection pressure, retained the resistant strains, spread among the same or different species of bacteria also increased #12;3 2.Potential Hazards of Resistant Bacteria · Resistant bacteria · Resistant gene

  18. MIGHTY BACTERIA IN A CIVILISED WORLD Prepared by Amy Wooding

    E-Print Network [OSTI]

    MIGHTY BACTERIA IN A CIVILISED WORLD Prepared by Amy Wooding The 2013 FABI/ CTHB UPwith of antibacterial products being advertised. The aim was to explore the idea created by these products that bacteria this by testing whether all bacteria can survive in every environment; will bacteria isolated from the UP

  19. Phase Preference by Active, Acetate-Utilizing Bacteria at the Rifle, CO Integrated Field Research Challenge Site

    SciTech Connect (OSTI)

    Kerkhoff, Lee; Williams, Kenneth H.; Long, Philip E.; McGuinness, L.

    2011-02-15

    Uranium contaminated groundwaters are a legacy concern for the U.S. Department of Energy. Previous experiments at the Rifle, Colorado Integrated Field Challenge (IFC) site have demonstrated that field-scale addition of acetate to groundwater reduces the ambient soluable uranium concentration, sequestering the radionuclide as uraninite. However, questions remain regarding which microorganism(s) are consuming this acetate and if active groundwater microorganisms are different from active particle-associated bacteria. In this report, 13-C acetate was used to assess the active microbes that synthesize DNA on 3 size fractions [coarse sand, fines (8-approximately 150 micron), groundwater (0.2-8 micron)] over a 24 -day time frame. Results indicated a stronger signal from 13-C acetate associated with the “fines” fraction compared with smaller amounts of 13-C uptake on the sand fraction and groundwater samples during the SIP incubations. TRFLP analysis of this 13-C-labeled DNA, indicated 31+ 9 OTU's with 6 peaks dominating the active profiles (166, 187, 210, 212, and 277 bp peaks using MnlI). Cloning/sequencing of the amplification products indicated a Geobacter-like group (187, 210, 212 bp) primarily synthesized DNA from acetate in the groundwater phase, an alpha Proteobacterium (166 bp) primarily grew on the fines/sands, and an Acinetobacter sp. (277 bp) utilized much of the 13C acetate in both groundwater and particle-associated phases. These findings will help to delineate the acetate utilization patterns of bacteria during field-scale acetate addition and can lead to improved methods for stimulating distinct microbial populations in situ.

  20. Carbon Flow of Heliobacteria Is Related More to Clostridia than to the Green Sulfur Bacteria*S

    E-Print Network [OSTI]

    Alvarez-Cohen, Lisa

    Carbon Flow of Heliobacteria Is Related More to Clostridia than to the Green Sulfur Bacteria*S, California 94720 The recently discovered heliobacteria are the only Gram-pos- itive photosynthetic bacteria the photosynthetic green sulfur bacteria (containing the type I reaction center) and Clostridia (forming heat

  1. Chemotactic selection of pollutant degrading soil bacteria

    DOE Patents [OSTI]

    Hazen, Terry C. (Augusta, GA)

    1994-01-01

    A method for identifying soil microbial strains which may be bacterial degraders of pollutants comprising the steps of placing a concentration of a pollutant in a substantially closed container, placing the container in a sample of soil for a period of time ranging from one minute to several hours, retrieving the container, collecting the contents of the container, and microscopically determining the identity of the bacteria present. Different concentrations of the pollutant can be used to determine which bacteria respond to each concentration. The method can be used for characterizing a polluted site or for looking for naturally occurring biological degraders of the pollutant. Then bacteria identified as degraders of the pollutant and as chemotactically attracted to the pollutant are used to inoculate contaminated soil. To enhance the effect of the bacteria on the pollutant, nutrients are cyclicly provided to the bacteria then withheld to alternately build up the size of the bacterial colony or community and then allow it to degrade the pollutant.

  2. Microbial Evolution, Diversity, and Ecology: A Decade of Ribosomal RNA Analysis of Uncultivated Microorganisms

    E-Print Network [OSTI]

    Vallino, Joseph J.

    - phologically distinctive, uncultivated bacteria; an important biotechnological process (wastewater treatment the definition of the major lineages (phyla or divisions [132]) within the three primary domains. This not only

  3. Metagenomic and Cultivation-Based Analysis of Novel Microorganisms and Functions in Metal-Contaminated Environments

    E-Print Network [OSTI]

    Yelton, Alexis Pepper

    2012-01-01

    acidophile important in acid mine drainage. Science 2000,III) Bacteria in Acid Mine Drainage Biofilms. Appl EnvironMicrobial communities in acid mine drainage. FEMS Microbiol

  4. The Social Lives of Microbes

    E-Print Network [OSTI]

    West, Stuart

    The Social Lives of Microbes Stuart A. West,1 Stephen P. Diggle,2 Angus Buckling,3 Andy Gardner,1 evolution Abstract Our understanding of the social lives of microbes has been revolu- tionized over the past that microbes indulge in a variety of social behaviors involving com- plex systems of cooperation, communication

  5. LIVING SOIL Master Gardener College

    E-Print Network [OSTI]

    Isaacs, Rufus

    LIVING SOIL Master Gardener College George W. Bird, Professor, MSU (June 9, 2012) #12;#12;Living Soil References G. W. Bird, Professor Michigan State University birdg@msu.edu http://www.ent.msu.edu/Directory/Facultypages/bird/tabid/133/Default.aspx · Brady, N. and R. Weil. 2002. Nature and Properties of Soils (13th ed) Prentice Hall

  6. Meso-scale turbulence in living fluids

    E-Print Network [OSTI]

    Henricus H. Wensink; Jörn Dunkel; Sebastian Heidenreich; Knut Drescher; Raymond E. Goldstein; Hartmut Löwen; Julia M. Yeomans

    2012-08-21

    Turbulence is ubiquitous, from oceanic currents to small-scale biological and quantum systems. Self-sustained turbulent motion in microbial suspensions presents an intriguing example of collective dynamical behavior amongst the simplest forms of life, and is important for fluid mixing and molecular transport on the microscale. The mathematical characterization of turbulence phenomena in active non-equilibrium fluids proves even more difficult than for conventional liquids or gases. It is not known which features of turbulent phases in living matter are universal or system-specific, or which generalizations of the Navier-Stokes equations are able to describe them adequately. Here, we combine experiments, particle simulations, and continuum theory to identify the statistical properties of self-sustained meso-scale turbulence in active systems. To study how dimensionality and boundary conditions affect collective bacterial dynamics, we measured energy spectra and structure functions in dense Bacillus subtilis suspensions in quasi-2D and 3D geometries. Our experimental results for the bacterial flow statistics agree well with predictions from a minimal model for self-propelled rods, suggesting that at high concentrations the collective motion of the bacteria is dominated by short-range interactions. To provide a basis for future theoretical studies, we propose a minimal continuum model for incompressible bacterial flow. A detailed numerical analysis of the 2D case shows that this theory can reproduce many of the experimentally observed features of self-sustained active turbulence.

  7. Biofuel from Bacteria and Sunlight: Shewanella as an Ideal Platform for Producing Hydrocarbons

    SciTech Connect (OSTI)

    None

    2010-01-01

    Broad Funding Opportunity Announcement Project: The University of Minnesota is developing clean-burning, liquid hydrocarbon fuels from bacteria. The University is finding ways to continuously harvest hydrocarbons from a type of bacteria called Shewanella by using a photosynthetic organism to constantly feed Shewanella the sugar it needs for energy and hydrocarbon production. The two organisms live and work together as a system. Using Shewanella to produce hydrocarbon fuels offers several advantages over traditional biofuel production methods. First, it eliminates many of the time-consuming and costly steps involved in growing plants and harvesting biomass. Second, hydrocarbon biofuels resemble current petroleum-based fuels and would therefore require few changes to the existing fuel refining and distribution infrastructure in the U.S.

  8. Denitrifying bacteria from the genus Rhodanobacter dominate bacterial communities in the highly contaminated subsurface of a nuclear legacy waste site

    SciTech Connect (OSTI)

    Green, Stefan [Florida State University; Prakash, Om [Florida State University; Jasrotia, Puja [Florida State University; Overholt, Will [Florida State University; Cardenas, Erick [Michigan State University, East Lansing; Hubbard, Daniela [Florida State University; Tiedje, James M. [Michigan State University, East Lansing; Watson, David B [ORNL; Schadt, Christopher Warren [ORNL; Brooks, Scott C [ORNL; Kostka, Joel [Florida State University

    2011-01-01

    The effect of long-term mixed-waste contamination, particularly uranium and nitrate, on the microbial community in the terrestrial subsurface was investigated at the field scale at the Oak Ridge Integrated Field Research Challenge (ORIFRC) site in Oak Ridge, TN. The abundance, community composition, and distribution of groundwater microorganisms were examined across the site during two seasonal sampling events. At representative locations, subsurface sediment was also examined from two boreholes, one sampled from the most heavily contaminated area of the site and another from an area with low contamination. A suite of DNA- and RNA-based molecular tools were employed for community characterization, including quantitative PCR of ribosomal RNA and nitrite reductase genes, community composition fingerprinting analysis, and high-throughput pyrotag sequencing of rRNA genes. The results demonstrate that pH is a major driver of the subsurface microbial community structure, and denitrifying bacteria from the genus Rhodanobacter (class Gammaproteobacteria) dominate at low pH. The relative abundance of bacteria from this genus was positively correlated with lower pH conditions, and these bacteria were abundant and active in the most highly contaminated areas. Other factors, such as concentration of nitrogen species, oxygen and sampling season did not appear to strongly influence the distribution of Rhodanobacter. Results indicate that these organisms are acid-tolerant denitrifiers, well suited to the acidic, nitrate-rich subsurface conditions, and pH is confirmed as a dominant driver of bacterial community structure in this contaminated subsurface environment.

  9. Blood Banking in Living Droplets

    E-Print Network [OSTI]

    Samot, Josh

    Blood banking has a broad public health impact influencing millions of lives daily. It could potentially benefit from emerging biopreservation technologies. However, although vitrification has shown advantages over traditional ...

  10. The Living Culture of Nigeria

    E-Print Network [OSTI]

    Biobaku, Saburi O.; Aniakor, Chike A.

    1977-01-01

    ImK ~IE.W THE uv:rn:; aJLWRE OF NIGERIA edited by Saburi 0 .Lagos: Thanas Nelson (Nigeria) Ltd. , Color Illustrations,86 The Living Cu'lture of Nigeria edited by Professor Saburi

  11. Human Occupancy as a Source of Indoor Airborne Bacteria

    E-Print Network [OSTI]

    Hospodsky, Denina

    Exposure to specific airborne bacteria indoors is linked to infectious and noninfectious adverse health outcomes. However, the sources and origins of bacteria suspended in indoor air are not well understood. This study ...

  12. ELECTRON HOLOGRAPHY OF NANOMAGNETS IN ROCKS AND BACTERIA

    E-Print Network [OSTI]

    Dunin-Borkowski, Rafal E.

    ELECTRON HOLOGRAPHY OF NANOMAGNETS IN ROCKS AND BACTERIA Rafal E. Dunin-Borkowski1 , Richard J-reversed thermo-remanent magnetization. #12;Magnetotactic bacteria provide the simplest example of the use

  13. Environmental Scientists Find Antibiotics, Bacteria, Resistance Genes in Feedlot Dust

    E-Print Network [OSTI]

    Rock, Chris

    Environmental Scientists Find Antibiotics, Bacteria, Resistance Genes in Feedlot Dust :: Texas Tech Today http://today.ttu.edu/2015/01/environmental-scientists-find-antibiotics Print Email + Font - Font Environmental Scientists Find Antibiotics, Bacteria, Resistance Genes

  14. Total synthesis of lysobactin : a natural product antibiotic active against methicillin and vancomycin resistant bacteria

    E-Print Network [OSTI]

    Guzman-Martinez, Aikomari

    2007-01-01

    has led bacteria to evolve and develop resistance toantibiotic resistance in many different kinds of bacteria.is a resistance mechanism utilized by MRSA and VRE bacteria.

  15. CRISPR--a widespread system that provides acquired resistance against phages in bacteria and archaea.

    E-Print Network [OSTI]

    Fenner, Marsha W

    2007-01-01

    confers acquired phage resistance in Bacteria and Archaea. Aacquired resistance against phages in bacteria and archaeaof phage resistance into sensitive industrial bacteria.

  16. The Structure of Fitness Landscapes in Antibiotic Resistant Bacteria : : Molecular Origins and Evolutionary Consequences

    E-Print Network [OSTI]

    Deris, John Barrett

    generically when bacteria exhibit resistance to translation-Mediated Drug Resistance in Bacteria: an Update. Drugs, 69(more antibiotics. Drug resistance in bacteria is genetically

  17. Selection against spurious promoter motifs correlates with translational efficiency across bacteria

    E-Print Network [OSTI]

    Froula, Jeffrey L.; Francino, M. Pilar

    2008-01-01

    translational efficiency across bacteria. Jeffrey L. Froulaof Eukarya, Archaea, and Bacteria reveals anticodon-sparingrachet in endosymbiotic bacteria. Proc Natl Acad Sci U S A

  18. When Bacteria Get Good: Progress, Purity, and the Making of Probiotics

    E-Print Network [OSTI]

    Yates, Allison

    2013-01-01

    45 Latour (1987). When Bacteria Get Good Bickler, S. W. , J.When Bacteria GetGood WHEN BACTERIA GET GOOD Progress, Purity, and the Making

  19. Computational prediction of type III secreted proteins from gram-negative bacteria

    E-Print Network [OSTI]

    Yang, Yang; Zhao, Jiayuan; Morgan, Robyn L; Ma, Wenbo; Jiang, Tao

    2010-01-01

    source Compared with other bacteria, Pseudomonas syringaeproteins from gram-negative bacteria Yang Yang 1 , Jiayuansystem in gram- negative bacteria that injects proteins (

  20. The effect of statin therapy on innate immune clearance of bacteria

    E-Print Network [OSTI]

    Chow, Ohn Aaron

    2010-01-01

    extracellular traps kill bacteria. Science 303, 1532-1535.extracellular traps to ensnare bacteria in septic blood. Natdigestion of Escherichia coli bacteria. J Immunol 169, 3172-

  1. Total synthesis of lysobactin : a natural product antibiotic active against methicillin and vancomycin resistant bacteria

    E-Print Network [OSTI]

    Guzman-Martinez, Aikomari

    2007-01-01

    Gram-Positive Resistant Bacteria. (IN PREPARATION. ) xxVancomycin Resistant Bacteria by Aikomarí Guzmán-Martíneznew strains of harmful bacteria that are resistant to

  2. Bioelectrical Perchlorate Reduction and Characterization of Novel Dissimilatory Perchlorate Reducing Bacteria

    E-Print Network [OSTI]

    Thrash, James Cameron

    2009-01-01

    per)chlorate-reducing bacteria and their phylogeneticper)chlorate- reducing bacteria. Appl. Environ. Microbiol.perchlorate-reducing bacteria Dechlorobacter hydrogenophilus

  3. To establish infection, pathogenic microorganisms have evolved many strategies to circumvent host defences and

    E-Print Network [OSTI]

    Nizet, Victor

    To establish infection, pathogenic microorganisms have evolved many strategies to circumvent host trafficking pathways to and from the host cell surface, which promotes pathogen entry, replication or escape of proteins such as Toll-like receptor 4 (TLR4) to phagosomes for receptor-mediated phagocytosis of pathogens8

  4. RESPONSES OF BENTHIC MICROORGANISMS (THECAMOEBIANS) TO OIL SANDS PROCESS-AFFECTED MATERIALS; PROVIDING

    E-Print Network [OSTI]

    Patterson, Timothy

    RESPONSES OF BENTHIC MICROORGANISMS (THECAMOEBIANS) TO OIL SANDS PROCESS-AFFECTED MATERIALS an important role in reclamation options for fluid tailings (OSPW/M) at surface oil sands operations. Through of oil sands operation have been compared. An index of response to stress has been compiled with the goal

  5. What is compost? Composting refers to biological decomposition and stabilization of organic materials by microorganisms under

    E-Print Network [OSTI]

    Mukhtar, Saqib

    What is compost? Composting refers to biological decomposition and stabilization of organic materials by microorganisms under aerobic conditions (in the presence of oxygen). During the composting is production of good-quality compost that is biologically stable, relatively uniform in appearance, free

  6. INTRODUCTION The contribution of micro-organisms to amorphous silica precipitation in modern

    E-Print Network [OSTI]

    Benning, Liane G.

    INTRODUCTION The contribution of micro-organisms to amorphous silica precipitation in modern, University of Guelph, Canada 3 Institute of Geological and Nuclear Sciences, Wairakei Research Centre, Taupo as geothermal energy sources and as a proxy to understanding the formation of epithermal ore deposits, which

  7. The impact of soil microorganisms on the global budget of 18

    E-Print Network [OSTI]

    sizes for CA- containing soil microorganisms. Including accelerated soil hydra- tion in global model Biotechnologie, Service de Biologie Ve´ge´tale et de Microbiologie Environnementale, Commissariat a` l'EnergieDepartment of Environmental Sciences and Energy Research, Weizmann Institute of Science, Rehovot, 76100, Israel; h

  8. Evidence That Mutation Is Universally Biased towards AT in Bacteria

    E-Print Network [OSTI]

    Petrov, Dmitri

    Evidence That Mutation Is Universally Biased towards AT in Bacteria Ruth Hershberg*, Dmitri A under severely relaxed selection are uniquely suitable for studying mutational biases in bacteria. We: Hershberg R, Petrov DA (2010) Evidence That Mutation Is Universally Biased towards AT in Bacteria. PLo

  9. A latitudinal diversity gradient in planktonic marine bacteria

    E-Print Network [OSTI]

    Brown, James H.

    A latitudinal diversity gradient in planktonic marine bacteria Jed A. Fuhrman* , Joshua A. Steele and attribute this to their high abundance and dispersal capabilities would suggest that bacteria, the smallest. Despite the high abundance and potentially high dispersal of bacteria, they exhibit geographic patterns

  10. Indigenous Bacteria from the Gut Microbiota Regulate Host Serotonin Biosynthesis

    E-Print Network [OSTI]

    Ismagilov, Rustem F.

    Article Indigenous Bacteria from the Gut Microbiota Regulate Host Serotonin Biosynthesis Graphical Abstract Highlights d Gut microbes regulate levels of 5-HT in the colon and blood d Spore-forming bacteria.cell.2015.02.047 #12;Article Indigenous Bacteria from the Gut Microbiota Regulate Host Serotonin

  11. Ancient bacteria show evidence of DNA repair Sarah Stewart Johnson*

    E-Print Network [OSTI]

    Nielsen, Rasmus

    Ancient bacteria show evidence of DNA repair Sarah Stewart Johnson* , Martin B. Hebsgaard , Torben for review June 14, 2007) Recent claims of cultivable ancient bacteria within sealed environ- ments highlight-term survival of bacteria sealed in frozen conditions for up to one million years. Our results show evidence

  12. A new process using a strain of Clostridium bacteria can

    E-Print Network [OSTI]

    Langendoen, Koen

    A new process using a strain of Clostridium bacteria can successfully convert biomass into butyl pipelines, facilitating transport and storage. Clostridium bacteria On the production side biobutanol has several disadvantages. It can be produced by fermentation using Clostridium bacteria, but this process

  13. Bacteria, Biofilms and Fluid Dynamics: Elementary Flows and Unexpected Phenomena

    E-Print Network [OSTI]

    Fisher, Frank

    Bacteria, Biofilms and Fluid Dynamics: Elementary Flows and Unexpected Phenomena Wednesday February the migration of bacteria along surfaces when exposed to a shear flow. In particular, we identify an unusual response where flow produces a directed motion of twitching bacteria in the upstream direction. (ii) We

  14. CHARACTERIZATION OF MAGNETIC NANOCRYSTALS FORMED BY MAGNETOTACTIC BACTERIA

    E-Print Network [OSTI]

    Dunin-Borkowski, Rafal E.

    CHARACTERIZATION OF MAGNETIC NANOCRYSTALS FORMED BY MAGNETOTACTIC BACTERIA M. Pósfai1 , T. Kasama2 fields remains poorly understood. Magnetotactic bacteria are the simplest organisms that use magnetite fields of iron oxide and iron sulfide nanoparticles in magnetotactic bacteria in order to understand

  15. BACTERIA-FILTERS: PERSISTENT PARTICLE FILTERS FOR BACKGROUND SUBTRACTION

    E-Print Network [OSTI]

    Peleg, Shmuel

    BACTERIA-FILTERS: PERSISTENT PARTICLE FILTERS FOR BACKGROUND SUBTRACTION Yair Movshovitz switch of bacteria between two states: A normal growing cell and a dormant but persistent cell after the stress is over, bacterial growth continues. Similar to bacteria, particles will switch between

  16. Physics 6, 61 (2013) The Aquatic Dance of Bacteria

    E-Print Network [OSTI]

    Goldstein, Raymond E.

    2013-01-01

    Physics 6, 61 (2013) Viewpoint The Aquatic Dance of Bacteria Igor Aranson Materials Science ­ Published May 28, 2013 Bacteria are among the oldest and most abundant liv- ing species on Earth maintain soil structure, ma- rine bacteria control the biochemistry and photosynthetic productivity

  17. Screening genomes of Gram-positive bacteria for

    E-Print Network [OSTI]

    Screening genomes of Gram-positive bacteria for double-glycine-motif- containing peptides Secreted-positive bacteria, the double-glycine (GG) motif plays a key role in many peptide secretion systems involved Microbiology Comment #12;peptides and class II bacteriocins, produced by streptococci and lactic acid bacteria

  18. TRITIUM INCORPORATION STUDIES IN PHOTO-SYNTHETIC BACTERIA

    E-Print Network [OSTI]

    Dehner, Thomas R.; Chan, W.-S.; Caple, Marianne B.; Calvin, M.

    2008-01-01

    STUDIES IN PHOTOSYN- s BACTERIA Thomas R. Dehner, H e n r ySTUDIES I N PHOTOSYNTmI"1C BACTERIA' BY Thomas R. ~ehner,'*rubm a f t e r the bacteria have been illuminated i n growth

  19. BACTERIA, FUNGI, AND UNICELLULAR ALGAE Blank page retained for pagination

    E-Print Network [OSTI]

    CHAPTER VI BACTERIA, FUNGI, AND UNICELLULAR ALGAE #12;Blank page retained for pagination #12;MARINE BACTERIA AND FUNGI IN THE GULF OF MEXICO I By CLAUDE E. ZOBELL, Scripps lrutitution of Oceano; Bavendamm 1932), there are very few published reports on bacteria and fungi in the nearby Gulf of Mexico

  20. Measurement of chlorite dismutase activities in perchlorate respiring bacteria

    E-Print Network [OSTI]

    Measurement of chlorite dismutase activities in perchlorate respiring bacteria Jianlin Xu*, Bruce E to chloride (ClO2 À ! ClÀ + O2) and is present in bacteria capable of cell respiration using perchlorate concentration (0.6 mM) to four other perchlorate respiring bacteria (PRB), and to one non-PRB (Pseudomonas

  1. Controlled Bacteria -Gold Nanorod Interactions for Enhancement of Optoacoustic Contrast

    E-Print Network [OSTI]

    Natelson, Douglas

    Controlled Bacteria - Gold Nanorod Interactions for Enhancement of Optoacoustic Contrast Anton as a model of positively charged gold surface for quantitative optoacoustic sensing in GNR-bacteria controlled agglomeration of contrast agents with the bacteria E.Coli and Vibrio Cholerae. For bacterial

  2. Microbiol Monogr (3) D. Schler: Magnetoreception and Magnetosomes in Bacteria

    E-Print Network [OSTI]

    Dunin-Borkowski, Rafal E.

    Microbiol Monogr (3) D. Schüler: Magnetoreception and Magnetosomes in Bacteria DOI 10 Abstract Magnetotactic bacteria can be regarded as model systems for studying the struc- tural, chemical Introduction Magnetotactic bacteria contain intracellular ferrimagnetic crystals that are typically 30­120 nm

  3. Biomineralization of carbonate and phosphate by moderately halophilic bacteria

    E-Print Network [OSTI]

    Gilli, Adrian

    Biomineralization of carbonate and phosphate by moderately halophilic bacteria M ´onica S-calcite; coprecipitated; moderately halophilic bacteria. Abstract We investigated the precipitation of carbonate and phosphate minerals by 19 species of moderately halophilic bacteria using media with variable Mg21 /Ca21

  4. Substrata Mechanical Stiffness Can Regulate Adhesion of Viable Bacteria

    E-Print Network [OSTI]

    Van Vliet, Krystyn J.

    Substrata Mechanical Stiffness Can Regulate Adhesion of Viable Bacteria Jenny A. Lichter,, M. Todd, 2008 The competing mechanisms that regulate adhesion of bacteria to surfaces and subsequent biofilm and hospital-acquired infections due to bacteria, there is considerable interest in better understanding

  5. 27/05/2012 21:13Fighting bacteria?s strength in numbers | Britney Spears New Video Page 1 of 2http://britneyspearsnewvideo.fruntrunnazproductions.com/194/fighting-bacterias-strength-in-numbers/

    E-Print Network [OSTI]

    West, Stuart

    27/05/2012 21:13Fighting bacteria?s strength in numbers | Britney Spears New Video Page 1 of 2http://britneyspearsnewvideo.fruntrunnazproductions.com/194/fighting-bacterias-strength-in-numbers/ Home Britney Spears New Video Fighting bacteria?s strength of Nottingham have opened the way for more accurate research into new ways to fight dangerous bacterial

  6. Comparative study of four fluorinated quinolones in susceptibility factors on microorganisms 

    E-Print Network [OSTI]

    Patel, Ketul R

    2013-02-22

    . This study hopes to prove an equal effectiveness of enrofloxacin and ciprofloxacin. Microbial susceptibility to drugs is measured by determining the MIC (Minimum Inhibitory Concentration). The MIC is measured based on the growth of the targeted bacteria...

  7. Biochemistry and physiology of anaerobic bacteria

    SciTech Connect (OSTI)

    2000-05-18

    We welcome you to The Power of Anaerobes. This conference serves two purposes. One is to celebrate the life of Harry D. Peck, Jr.,who was born May 18, 1927 and would have celebrated his 73rd birthday at this conference. He died November 20, 1998. The second is to gather investigators to exchange views within the realm of anaerobic microbiology, an area in which tremendous progress has been seen during recent years. It is sufficient to mention discoveries of a new form of life (the archaea), hyper or extreme thermophiles, thermophilic alkaliphiles and anaerobic fungi. With these discoveries has come a new realization about physiological and metabolic properties of microorganisms, and this in turn has demonstrated their importance for the development, maintenance and sustenance of life on Earth.

  8. Nonequilibrium dissipation in living oocytes

    E-Print Network [OSTI]

    Étienne Fodor; Wylie W. Ahmed; Maria Almonacid; Matthias Bussonnier; Nir S. Gov; Marie-Hélène Verlhac; Timo Betz; Paolo Visco; Frédéric van Wijland

    2015-11-03

    Living organisms are inherently out-of-equilibrium systems. We employ new developments in stochastic energetics and rely on a minimal microscopic model to predict the amount of mechanical energy dissipated by such dynamics. Our model includes complex rheological effects and nonequilibrium stochastic forces. By performing active microrheology and tracking micron-sized vesicles in the cytoplasm of living oocytes, we provide unprecedented measurements of the spectrum of dissipated energy. We show that our model is fully consistent with the experimental data, and we use it to offer predictions for the injection and dissipation energy scales involved in active fluctuations.

  9. Natural Oil Production from Microorganisms: Bioprocess and Microbe Engineering for Total Carbon Utilization in Biofuel Production

    SciTech Connect (OSTI)

    2010-07-15

    Electrofuels Project: MIT is using carbon dioxide (CO2) and hydrogen generated from electricity to produce natural oils that can be upgraded to hydrocarbon fuels. MIT has designed a 2-stage biofuel production system. In the first stage, hydrogen and CO2 are fed to a microorganism capable of converting these feedstocks to a 2-carbon compound called acetate. In the second stage, acetate is delivered to a different microorganism that can use the acetate to grow and produce oil. The oil can be removed from the reactor tank and chemically converted to various hydrocarbons. The electricity for the process could be supplied from novel means currently in development, or more proven methods such as the combustion of municipal waste, which would also generate the required CO2 and enhance the overall efficiency of MIT’s biofuel-production system.

  10. Saving Lives and Mitigating Losses

    E-Print Network [OSTI]

    Duchowski, Andrew T.

    Saving Lives and Mitigating Losses Wind and Structural Engineering Research Facility #12;Clemson University's Wind and Structural Engineering Research (WiSER) Facility is a premier laboratory for the study of wind effects on structures. Testing to assess the structural performance of buildings and bridges can

  11. Turning bacteria suspensions into a "superfluid"

    E-Print Network [OSTI]

    Héctor Matías López; Jérémie Gachelin; Carine Douarche; Harold Auradou; Eric Clément

    2015-03-18

    The rheological response under simple shear of an active suspension of Escherichia coli is determined in a large range of shear rates and concentrations. The effective viscosity and the time scales characterizing the bacterial organization under shear are obtained. In the dilute regime, we bring evidences for a low shear Newtonian plateau characterized by a shear viscosity decreasing with concentration. In the semi-dilute regime, for particularly active bacteria, the suspension display a "super-fluid" like transition where the viscous resistance to shear vanishes, thus showing that macroscopically, the activity of pusher swimmers organized by shear, is able to fully overcome the dissipative effects due to viscous loss.

  12. Turning bacteria suspensions into a "superfluid"

    E-Print Network [OSTI]

    López, Héctor Matías; Douarche, Carine; Auradou, Harold; Clément, Eric

    2015-01-01

    The rheological response under simple shear of an active suspension of Escherichia coli is determined in a large range of shear rates and concentrations. The effective viscosity and the time scales characterizing the bacterial organization under shear are obtained. In the dilute regime, we bring evidences for a low shear Newtonian plateau characterized by a shear viscosity decreasing with concentration. In the semi-dilute regime, for particularly active bacteria, the suspension display a "super-fluid" like transition where the viscous resistance to shear vanishes, thus showing that macroscopically, the activity of pusher swimmers organized by shear, is able to fully overcome the dissipative effects due to viscous loss.

  13. High-throughput experimental and computational tools for exploring immunity and the microbiome

    E-Print Network [OSTI]

    Papa, Eliseo

    2012-01-01

    Humans live in association with trillions of microbes and yet we know remarkably little about their symbiotic relationship. The role these microorganisms have in humans has been characterized only in the case of few bacteria ...

  14. Delayed accumulation of intestinal coliform bacteria enhances life span and stress resistance in Caenorhabditis elegans fed respiratory deficient E. coli

    E-Print Network [OSTI]

    2012-01-01

    coliform bacteria enhances life span and stress resistancecoliform bacteria enhances life span and stress resistance

  15. Purple bacteria and quantum Fourier transform

    E-Print Network [OSTI]

    Samir Lipovaca

    2007-02-22

    The LH-II of purple bacteria Rhodospirillum (Rs.) molischianum and Rhodopseudomonas (Rps.) acidophila adopts a highly symmetrical ring shape, with a radius of about 7 nm. In the case of Rps. acidophila the ring has a ninefold symmetry axis, and in LH-II from Rs. molischianum the ring has an eightfold symmetry axis. These rings are found to exibit two bands of excitons. A simplified mathematical description of the exciton states is given in Hu, X. & Schulten, K. (1997) Physics Today 50, 28-34. Using this description, we will show, by suitable labeling of the lowest energy (Qy) excited states of individual BChls, that the resulting exciton states are the quantum Fourier transform of the BChls excited states. For Rs. molischianum ring exciton states will be modeled as the four qubit quantum Fourier transform and the explicit circuit will be derived. Exciton states for Rps. acidophila ring cannot be modeled with an integer number of qubits. Both quantum Fourier transforms are instances of the hidden subgroup problem and this opens up a possibility that both purple bacteria implement an efficient quantum circuit for light harvesting.

  16. Detection of phenols using engineered bacteria

    DOE Patents [OSTI]

    Wise, Arlene A. (Philadelphia, PA); Kuske, Cheryl R. (Los Alamos, NM); Terwilliger, Thomas C. (Santa Fe, NM)

    2007-12-04

    Detection of phenols using engineered bacteria. A biosensor can be created by placing a reporter gene under control of an inducible promoter. The reporter gene produces a signal when a cognate transcriptional activator senses the inducing chemical. Creation of bacterial biosensors is currently restricted by limited knowledge of the genetic systems of bacteria that catabolize xenobiotics. By using mutagenic PCR to change the chemical specificity of the Pseudomonas species CF600 DmpR protein, the potential for engineering novel biosensors for detection of phenols has been demonstrated. DmpR, a well-characterized transcriptional activator of the P. CF600's dmp operon mediates growth on simple phenols. Transcription from Po, the promoter heading the dmp operon, is activated when the sensor domain of DmpR interacts with phenol and mono-substituted phenols. By altering the sensor domain of the DmpR, a group of DmpR derivatives that activate transcription of a Po-lacZ fusion in response to eight of the EPA's eleven priority pollutant phenols has been created. The assays and the sensor domain mutations that alter the chemical specificity of DmpR is described.

  17. Detection of phenols using engineered bacteria

    DOE Patents [OSTI]

    Wise, Arlene A.; Kuske, Cheryl R.; Terwilliger, Thomas C.

    2004-08-10

    Detection of phenols using engineered bacteria. A biosensor can be created by placing a reporter gene under control of an inducible promoter. The reporter gene produces a signal when a cognate transcriptional activator senses the inducing chemical. Creation of bacterial biosensors is currently restricted by limited knowledge of the genetic systems of bacteria that catabolize xenobiotics. By using mutagenic PCR to change the chemical specificity of the Pseudomonas species CF600 DmpR protein, the potential for engineering novel biosensors for detection of phenols has been demonstrated. DmpR, a well-characterized transcriptional activator of the P. CF600's dmp operon mediates growth on simple phenols. Transcription from Po, the promoter heading the dmp operon, is activated when the sensor domain of DmpR interacts with phenol and mono-substituted phenols. By altering the sensor domain of the DmpR, a group of DmpR derivatives that activate transcription of a Po-lacZ fusion in response to eight of the EPA's eleven priority pollutant phenols has been created. The assays and the sensor domain mutations that alter the chemical specificity of DmpR is described.

  18. Starvation-survival of subsurface bacteria

    SciTech Connect (OSTI)

    Magill, N.G.

    1988-01-01

    The ability of four subsurface isolates to survive starvation was examined and the results were compared to survival curves obtained for Escherichia coli B and Serratia marcescens. To examine the starvation-survival phenomenon further, several experimental parameters including nutritional history, initial cell density, growth phase, temperature of growth and starvation, and aeration. Nutritional history, initial cell density, and growth phases of the cells had some effect on the ability of these bacteria to survive whereas temperature and limited aeration had no effect under the conditions tested. No conditions were found where E. coli B or Serratia marcescens died rapidly or where less than 10% of the original cell number of viable cells remained. Because the apparent survival of these bacteria may be due to cryptic growth, cross-feeding experiments with {sup 14}C-labeled cells and unlabeled cells were carried out with E. coli B and Pseudomonas Lula V. Leaked extracellular {sup 14}C-compounds were not used for growth or maintenance energy, and were not taken up by either bacterium. Cryptic growth did not occur; the cells were truly starving under the experimental conditions used.

  19. Transfer of noncoding DNA drives regulatory rewiring in bacteria

    E-Print Network [OSTI]

    Oren, Yaara

    Understanding the mechanisms that generate variation is a common pursuit unifying the life sciences. Bacteria represent an especially striking puzzle, because closely related strains possess radically different metabolic ...

  20. Rapid quantification of mutant fitness in diverse bacteria by...

    Office of Scientific and Technical Information (OSTI)

    by sequencing randomly bar-coded transposons Transposon mutagenesis with next-generation sequencing (TnSeq) is a powerful approach to annotate gene function in bacteria, but...

  1. Copy of Synthetic Biology of Novel Thermophilic Bacteria for...

    Office of Scientific and Technical Information (OSTI)

    Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). Citation Details In-Document Search Title: Copy...

  2. Attachment and detachment of microorganisms as related to sampling carcasses and meat products 

    E-Print Network [OSTI]

    Butler, Jacqueline Love

    1980-01-01

    of Advisory Committee: Dr. C. Vanderzant A model system was developed to study attachment and de- t-chment of' bacteria with pork skin and muscle surfaces of beef and lamb carcasses. The technique involved embedding pork skin and beef and lamb muscle... medium, although in some cases attachment continued to occur over a $0-min period. Gram-negative motile bacteria had greater force of attachment than gram-positive non-motile species. Temperature and pH of the attachment medium had little eff...

  3. Energy conversion in Purple Bacteria Photosynthesis

    E-Print Network [OSTI]

    Caycedo-Soler, Felipe; Quiroga, Luis; Zhao, Guannan; Johnson, Neil F

    2011-01-01

    The study of how photosynthetic organisms convert light offers insight not only into nature's evolutionary process, but may also give clues as to how best to design and manipulate artificial photosynthetic systems -- and also how far we can drive natural photosynthetic systems beyond normal operating conditions, so that they can harvest energy for us under otherwise extreme conditions. In addition to its interest from a basic scientific perspective, therefore, the goal to develop a deep quantitative understanding of photosynthesis offers the potential payoff of enhancing our current arsenal of alternative energy sources for the future. In the following Chapter, we consider the trade-off between dynamics, structure and function of light harvesting membranes in Rps. Photometricum purple bacteria, as a model to highlight the priorities that arise when photosynthetic organisms adapt to deal with the ever-changing natural environment conditions.

  4. Energy conversion in Purple Bacteria Photosynthesis

    E-Print Network [OSTI]

    Felipe Caycedo-Soler; Ferney J. Rodriguez; Luis Quiroga; Guannan Zhao; Neil F. Johnson

    2011-07-01

    The study of how photosynthetic organisms convert light offers insight not only into nature's evolutionary process, but may also give clues as to how best to design and manipulate artificial photosynthetic systems -- and also how far we can drive natural photosynthetic systems beyond normal operating conditions, so that they can harvest energy for us under otherwise extreme conditions. In addition to its interest from a basic scientific perspective, therefore, the goal to develop a deep quantitative understanding of photosynthesis offers the potential payoff of enhancing our current arsenal of alternative energy sources for the future. In the following Chapter, we consider the trade-off between dynamics, structure and function of light harvesting membranes in Rps. Photometricum purple bacteria, as a model to highlight the priorities that arise when photosynthetic organisms adapt to deal with the ever-changing natural environment conditions.

  5. Measuring Interference Between Live Datacenter Applications

    E-Print Network [OSTI]

    Cortes, Corinna

    Measuring Interference Between Live Datacenter Applications Melanie Kambadur Columbia University in datacenters due to contention over shared hardware resources. Unfortunately, understanding interference in live datacenters is more difficult than in controlled environments or on simpler architectures. Most

  6. Solo living across the adult lifecourse 

    E-Print Network [OSTI]

    Smith, Adam; Wasoff, Fran; Jamieson, Lynn

    This study has created snapshots of solo living in contemporary society and developed an understanding of the social and economic factors involved in transitions in and out of solo living.

  7. Characteristics of cyclic AMP transport by marine bacteria

    SciTech Connect (OSTI)

    Ammerman, J.W.; Azam, F.

    1987-12-01

    Uptake and autoradiography experiments with natural populations of marine bacteria, sea water cultures, and cultured isolates showed that the high-affinity cyclic AMP transport system in marine bacteria has stringent structural requirements, is found in a minority of cells in mixed bacterial assemblages, and appears to be related to the culture growth state.

  8. Magnetic minerals produced by magnetotactic bacteria Balzs Arat1

    E-Print Network [OSTI]

    Dunin-Borkowski, Rafal E.

    Magnetic minerals produced by magnetotactic bacteria Balázs Arató1 , Mihály Pósfai1 and Rafal E-controlled mineralization Abstract. Magnetotactic bacteria produce intracellular magnetic minerals that have distinct for studying the biological membrane around the mineral grains. Our goals were to deduce the possible growth

  9. 17 -Estradiol-Degrading Bacteria Isolated from Activated Sludge

    E-Print Network [OSTI]

    Chu, Kung-Hui "Bella"

    17 -Estradiol-Degrading Bacteria Isolated from Activated Sludge C H A N G - P I N G Y U , H Y U N G-degrading bacteria (strains KC1-14) were isolated from activated sludge of a wastewater treatment plant

  10. The Impact of Different Antibiotic Regimens on the Emergence of Antimicrobial-Resistant Bacteria

    E-Print Network [OSTI]

    Ruan, Shigui

    The Impact of Different Antibiotic Regimens on the Emergence of Antimicrobial-Resistant Bacteria-resistant bacteria is a major public health threat. Infections caused by antimicrobial-resistant bacteria by antimicrobial-susceptible bacteria. The emergence and spread of these bacteria is complex and requires

  11. Identification of bacteria synthesizing ribosomal RNA in response to uranium addition during biostimulation at the Rifle, CO Integrated Field Research site

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    McGuinness, Lora R.; Wilkins, Michael J.; Williams, Kenneth H.; Long, Philip E.; Kerkhof, Lee J.; Boyanov, Maxim I.

    2015-09-18

    Understanding which organisms are capable of reducing uranium at historically contaminated sites provides crucial information needed to evaluate treatment options and outcomes. One approach is determination of the bacteria which directly respond to uranium addition. In this research, uranium amendments were made to groundwater samples from a site of ongoing biostimulation with acetate. The active microbes in the planktonic phase were deduced by monitoring ribosomes production via RT-PCR. The results indicated several microorganisms were synthesizing ribosomes in proportion with uranium amendment up to 2 ?M. Concentrations of U (VI) >2 ?M were generally found to inhibit ribosome synthesis. Two activemore »bacteria responding to uranium addition in the field were close relatives of Desulfobacter postgateii and Geobacter bemidjiensis. Since RNA content often increases with growth rate, our findings suggest it is possible to rapidly elucidate active bacteria responding to the addition of uranium in field samples and provides a more targeted approach to stimulate specific populations to enhance radionuclide reduction in contaminated sites.« less

  12. Live Fire Range Environmental Assessment

    SciTech Connect (OSTI)

    1993-08-01

    The Central Training Academy (CTA) is a DOE Headquarters Organization located in Albuquerque, New Mexico, with the mission to effectively and efficiently educate and train personnel involved in the protection of vital national security interests of DOE. The CTA Live Fire Range (LFR), where most of the firearms and tactical training occurs, is a complex separate from the main campus. The purpose of the proposed action is to expand the LFR to allow more options of implementing required training. The Department of Energy has prepared this Environmental Assessment (EA) for the proposed construction and operation of an expanded Live Fire Range Facility at the Central Training Academy in Albuquerque, New Mexico. Based on the analysis in the EA, DOE has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an environmental impact statement is not required and DOE is issuing this Finding of No Significant Impact (FONSI).

  13. Detection and quantification of waterborne microorganisms using an image cytometer based on angular spatial frequency processing

    E-Print Network [OSTI]

    Pérez, Juan Miguel; Martínez, Pedro; Pruneri, Valerio

    2015-01-01

    We introduce a new image cytometer design for detection of very small particulate and demonstrate its capability in water analysis. The device is a compact microscope composed of off--the--shelf components, such as a light emitting diode (LED) source, a complementary metal--oxide--semiconductor (CMOS) image sensor, and a specific combination of optical lenses that allow, through an appropriate software, Fourier transform processing of the sample volume. Waterborne microorganisms, such as Escherichia coli (E. coli), Legionella pneumophila (L. pneumophila) and Phytoplankton, are detected by interrogating the volume sample either in a fluorescent or label-free mode, i.e. with or without fluorescein isothiocyanate (FITC) molecules attached to the micro-organisms, respectively. We achieve a sensitivity of 50 CFU/ml, which can be further increased to 0.2 CFU/ml by pre-concentrating an initial sample volume of 500 ml with an ad hoc fluidic system. We also prove the capability of the proposed image cytometer of diffe...

  14. Bacteria Induced Split Anergy in NK Cells Drive Maturation, Differentiation and Resistance of Healthy and Transformed Stem Cells

    E-Print Network [OSTI]

    Bui, Vickie Tam Doan

    2014-01-01

    of probiotic bacteria in maturation and resistance of Oralwithout probiotic bacteria induced resistance of OSCSCs, MIAof probiotic bacteria in maturation and resistance of Oral

  15. Metal Cycling by Bacteria: Moving Electrons Around

    SciTech Connect (OSTI)

    Nealson, Ken

    2009-07-06

    About 20 years ago, Shewanella oneidensis MR-1 was isolated from a manganese-rich lack in upstate New York, and subsequently shown to utilize solid forms of oxidized manganese or iron as an electron acceptor. Recent studies of metal-reducing bacterial have unveiled a number of unexpected properties of microbes that have enlarged our view of microbes and their role(s) in natural ecosystems. For example, the processes of metal reduction themselves are fundamental to the carbon cycle in many lakes and sediments, where iron and manganese account for the major portion of organic carbon oxidation in many sediments. On more modest spatial scales, iron and manganese reduction can be linked to the oxidation of a wide variety of carbon compounds, many of them recalcitrant and/or toxic. One remarkable property of metal reducers is their ability to reduce solid, often highly crystalline substrates such as iron and manganese oxides and oxyhydroxides. It is now clear that this is done via the utilization of enzymes located on the outer wall of the bacteria - enzymes that apparently interact directly with these solid substrates. Molecular and genomic studies combined have revealed the genes and protoeins responsible for these activities, and many facets of the regulation. This talk focuses on the general features and properties of these remarkable organisms that seem to communicate via electron transfer across a wide variety of soluable, insoluable, and even "inert" substrates, and the way that these processes may be mechanistically linked.

  16. Metal Cycling by Bacteria: Moving Electrons Around

    ScienceCinema (OSTI)

    Nealson, Ken

    2010-01-08

    About 20 years ago, Shewanella oneidensis MR-1 was isolated from a manganese-rich lack in upstate New York, and subsequently shown to utilize solid forms of oxidized manganese or iron as an electron acceptor. Recent studies of metal-reducing bacterial have unveiled a number of unexpected properties of microbes that have enlarged our view of microbes and their role(s) in natural ecosystems. For example, the processes of metal reduction themselves are fundamental to the carbon cycle in many lakes and sediments, where iron and manganese account for the major portion of organic carbon oxidation in many sediments. On more modest spatial scales, iron and manganese reduction can be linked to the oxidation of a wide variety of carbon compounds, many of them recalcitrant and/or toxic. One remarkable property of metal reducers is their ability to reduce solid, often highly crystalline substrates such as iron and manganese oxides and oxyhydroxides. It is now clear that this is done via the utilization of enzymes located on the outer wall of the bacteria - enzymes that apparently interact directly with these solid substrates. Molecular and genomic studies combined have revealed the genes and protoeins responsible for these activities, and many facets of the regulation. This talk focuses on the general features and properties of these remarkable organisms that seem to communicate via electron transfer across a wide variety of soluable, insoluable, and even "inert" substrates, and the way that these processes may be mechanistically linked.

  17. 1. The derivative of y = mm“ at x = 2. A bacteria culture starts with ...

    E-Print Network [OSTI]

    The derivative of y = mm“ at x = 2. A bacteria culture starts with 200 bacteria and grows at a rate proportional to its size. After 2 hours there were 400 bacteria.

  18. Native California soils are selective reservoirs for multidrug-resistant bacteria

    E-Print Network [OSTI]

    Sachs, Joel

    Native California soils are selective reservoirs for multidrug-resistant bacteria Amanda C of antibiotic resistance in Bradyrhizobium (alphaproteobacteria). Bradyrhizobium are cosmopolitan bacteria bacteria can exhibit extensive antibiotic re- sistomes and act as reservoirs of important antibiotic

  19. Comparative genomics of metabolic capacities of regulons controlled by cis-regulatory RNA motifs in bacteria

    E-Print Network [OSTI]

    Sun, Eric I; Leyn, Semen A; Kazanov, Marat D; Saier, Milton H; Novichkov, Pavel S; Rodionov, Dmitry A

    2013-01-01

    candidate structured RNAs from bacteria, archaea, and theirT-box regulatory systems in bacteria. RNA 2008, 15. Wels M,and transport genes in bacteria: yet another RNA riboswitch?

  20. Biomarker evidence for green and purple sulphur bacteria in a stratified Palaeoproterozoic sea

    E-Print Network [OSTI]

    Brocks, Jochen J.

    Biomarker evidence for green and purple sulphur bacteria in a stratified Palaeoproterozoic sea waters, hostile to eukaryotic algae. Phototrophic purple sulphur bacteria (Chromatiaceae) were detected-existed with communities of green sulphur bacteria (Chlorobiaceae). Collectively, the biomarkers support mounting evidence

  1. Quantum Process in Living Cells

    E-Print Network [OSTI]

    Robert W. Finkel

    2012-10-04

    Quantum processes have been confirmed for various biological phenomena. Here we model a quantum process in cells based on coherent waves of established ultrafast energy transfers in water. We compute wave speed, ~156 km/s, and wavelength, ~9.3 nm, and determine that the waves retain local coherence. The model is compared with observations and diverse numerical applications lend support to the hypothesis that rapid energy transfers in water are characteristic of living cells. Close agreements are found for the dipole moment of water dimers, microwave radiation on yeast, and the Kleiber law of metabolic rates. We find a sphere with diameter ~20 nm is a lower bound for life in this theory. The quantum properties of the model suggest that cellular chemistry favors reactions that support perpetuation of the energy waves

  2. Bacteria Total Maximum Daily Load Task Force Final Report 

    E-Print Network [OSTI]

    Jones, C. Allan; Wagner, Kevin; Di Giovanni, George; Hauck, Larry; Mott, Joanna; Rifai, Hanadi; Srinivasan, Raghavan; Ward, George; Wythe, Kathy

    2009-01-01

    stream_source_info TR-341 Bacteria TMDL Task Force Report Draft Four 6.4.07.pdf.txt stream_content_type text/plain stream_size 344770 Content-Encoding ISO-8859-1 stream_name TR-341 Bacteria TMDL Task Force Report Draft Four 6....4.07.pdf.txt Content-Type text/plain; charset=ISO-8859-1 TR-341 2009 Bacteria Total Maximum Daily Load Task Force Final Report By C. Allan Jones and Kevin Wagner, Texas Water Resources...

  3. Modeling magnetosensitive ion channels in viscoelastic environment of living cells

    E-Print Network [OSTI]

    Igor Goychuk

    2015-10-01

    We propose and study a model of hypothetical magnetosensitive ionic channels which are long thought to be a possible candidate to explain the influence of weak magnetic fields on living organisms ranging from magnetotactic bacteria to fishes, birds, rats, bats and other mammals including humans. The core of the model is provided by a short chain of magnetosomes serving as a sensor which is coupled by elastic linkers to the gating elements of ion channels forming a small cluster in the cell membrane. The magnetic sensor is fixed by one end on cytoskeleton elements attached to the membrane and is exposed to viscoelastic cytosol. Its free end can reorient stochastically and subdiffusively in viscoelastic cytosol responding to external magnetic field changes and open the gates of coupled ion channels. The sensor dynamics is generally bistable due to bistability of the gates which can be in two states with probabilities which depend on the sensor orientation. For realistic parameters, it is shown that this model channel can operate in the magnetic field of Earth for a small number (5 to 7) of single-domain magnetosomes constituting the sensor rod each of which has a typical size found in magnetotactic bacteria and other organisms, or even just one sufficiently large nanoparticle of a characteristic size also found in nature. It is shown that due to viscoelasticity of medium the bistable gating dynamics generally exhibits power law and stretched exponential distributions of the residence times of the channels in their open and closed states. This provides a generic physical mechanism for explanation of the origin of such anomalous kinetics for other ionic channels whose sensors move in viscoelastic environment provided by either cytosol or biological membrane, in a quite general context, beyond the fascinating hypothesis of magnetosensitive ionic channels we explore.

  4. Single molecule resolution of the antimicrobial action of quantum dot-labeled sushi peptide on live bacteria

    E-Print Network [OSTI]

    Ding, Jeak Ling

    Background: Antimicrobial peptides are found in all kingdoms of life. During the evolution of multicellular organisms, antimicrobial peptides were established as key elements of innate immunity. Most antimicrobial peptides ...

  5. Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity

    DOE Patents [OSTI]

    Premuzic, E.T.; Lin, M.

    1996-02-20

    This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70 C to 90 C, at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%. 68 figs.

  6. Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity

    DOE Patents [OSTI]

    Premuzic, Eugene T. (East Moriches, NY); Lin, Mow (Rocky Point, NY)

    1996-02-20

    This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70.degree. C. to 90.degree. C., at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%.

  7. Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels

    SciTech Connect (OSTI)

    Kuk Lee, Sung; Chou, Howard; Ham, Timothy S.; Soon Lee, Taek; Keasling, Jay D.

    2009-12-02

    The ability to generate microorganisms that can produce biofuels similar to petroleum-based transportation fuels would allow the use of existing engines and infrastructure and would save an enormous amount of capital required for replacing the current infrastructure to accommodate biofuels that have properties significantly different from petroleum-based fuels. Several groups have demonstrated the feasibility of manipulating microbes to produce molecules similar to petroleum-derived products, albeit at relatively low productivity (e.g. maximum butanol production is around 20 g/L). For cost-effective production of biofuels, the fuel-producing hosts and pathways must be engineered and optimized. Advances in metabolic engineering and synthetic biology will provide new tools for metabolic engineers to better understand how to rewire the cell in order to create the desired phenotypes for the production of economically viable biofuels.

  8. How sulphate-reducing microorganisms cope with stress: Lessons from systems biology

    SciTech Connect (OSTI)

    Zhou, J.; He, Q.; Hemme, C.L.; Mukhopadhyay, A.; Hillesland, K.; Zhou, A.; He, Z.; Nostrand, J.D. Van; Hazen, T.C.; Stahl, D.A.; Wall, J.D.; Arkin, A.P.

    2011-04-01

    Sulphate-reducing microorganisms (SRMs) are a phylogenetically diverse group of anaerobes encompassing distinct physiologies with a broad ecological distribution. As SRMs have important roles in the biogeochemical cycling of carbon, nitrogen, sulphur and various metals, an understanding of how these organisms respond to environmental stresses is of fundamental and practical importance. In this Review, we highlight recent applications of systems biology tools in studying the stress responses of SRMs, particularly Desulfovibrio spp., at the cell, population, community and ecosystem levels. The syntrophic lifestyle of SRMs is also discussed, with a focus on system-level analyses of adaptive mechanisms. Such information is important for understanding the microbiology of the global sulphur cycle and for developing biotechnological applications of SRMs for environmental remediation, energy production, biocorrosion control, wastewater treatment and mineral recovery.

  9. Micro-scale interactions between chemotactic bacteria and algae

    E-Print Network [OSTI]

    Vahora, Nisha

    2010-01-01

    Traditional views of marine environments describe the ocean pelagic zone as a homogeneous nutrient-poor environment. Heterotrophic marine bacteria that have evolved high-energy mechanisms for swimming abilities and sensing ...

  10. In Vivo Gene Expression Dynamics of Tumor-Targeted Bacteria

    E-Print Network [OSTI]

    Danino, Tal

    The engineering of bacteria to controllably deliver therapeutics is an attractive application for synthetic biology. While most synthetic gene networks have been explored within microbes, there is a need for further ...

  11. Small Talk: Cell-to-Cell Communication in Bacteria

    ScienceCinema (OSTI)

    Bassler, Bonnie [Princeton University, Princeton, New Jersey, United States

    2010-01-08

    Cell-cell communication in bacteria involves the production, release, and subsequent detection of chemical signaling molecules called autoinducers. This process, called quorum sensing, allows bacteria to regulate gene expression on a population-wide scale. Processes controlled by quorum sensing are usually ones that are unproductive when undertaken by an individual bacterium but become effective when undertaken by the group. For example, quorum sensing controls bioluminescence, secretion of virulence factors, biofilm formation, sporulation, and the exchange of DNA. Thus, quorum sensing is a mechanism that allows bacteria to function as multi-cellular organisms. Bacteria make, detect, and integrate information from multiple autoinducers, some of which are used exclusively for intra-species communication while others enable communication between species. Research is now focused on the development of therapies that interfere with quorum sensing to control bacterial virulence.

  12. Top 10 plant pathogenic bacteria in molecular plant pathology.

    E-Print Network [OSTI]

    2012-01-01

    Foster, G.D. (2012) The top 10 fungal pathogens in molecularBLACKWELL PUBLISHING LTD Top 10 plant pathogenic bacteriaC. and Foster, G.D. (2011) Top 10 plant viruses in molecular

  13. Bacteria recovered from endometritis and pyometra in the beef cow 

    E-Print Network [OSTI]

    Mikulec, Rashel Thi

    1999-01-01

    One hundred and one uteri from beef cows with pyometra were collected from a slaughterhouse. Samples of uterine exudate were cultured for aerobic, microaerophilic, and anaerobic bacteria, and also tested for Trichomonas spp. A section of uterine...

  14. Foodborne Sources of Bacteria Associated With Human Obesity 

    E-Print Network [OSTI]

    McElhany, Katherine Grace

    2009-09-30

    Recently published research has suggested that the microbial ecology of the digestive system may play a role in obesity. Obese people have been shown to have a higher proportion of bacteria from the Firmicutes division and ...

  15. Lancaster Live/Work Townhome Prototype

    SciTech Connect (OSTI)

    2010-04-01

    This case study describes development of a prototype live-work townhome that is highly efficient at 45% energy savings (95% counting photovoltaic system).

  16. Nuclear Physics Technology Saves Lives | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nuclear Physics Technology Saves Lives January 11, 2006 Listen to this story Ribbon With early detection, breast cancer can often be treated successfully. There are over two...

  17. Extending the Operating Lives of Materials

    ScienceCinema (OSTI)

    Paul Jablonski

    2010-09-01

    Metallurgist Paul Jablonski discusses his role in developing processes that extend the operating temperatures and operating lives of materials used in energy applications.

  18. Connecticut Weatherization Project Improves Lives, Receives National...

    Broader source: Energy.gov (indexed) [DOE]

    Several energy-efficient improvements made to a senior care center in New Milford, Connecticut, are helping residents live healthier and more comfortable lifestyles. The upgrade...

  19. Material and method for promoting the growth of anaerobic bacteria

    DOE Patents [OSTI]

    Adler, Howard I. (128 Indian La., Oak Ridge, TN 37830)

    1984-01-01

    A material and method for promoting the growth of anaerobic bacteria which includes a nutrient media containing a hydrogen donor and sterile membrane fragments of bacteria having an electron transfer system which reduces oxygen to water. Dissolved oxygen in the medium is removed by adding the sterile membrane fragments to the nutrient medium and holding the medium at a temperature of about 10.degree. to about 60.degree. C. until the dissolved oxygen is removed.

  20. A C. elegans-based foam for rapid on-site detection of residual live virus.

    SciTech Connect (OSTI)

    Negrete, Oscar A.; Branda, Catherine; Hardesty, Jasper O. E. (Sandia National Laboratories, Albuquerque, NM); Tucker, Mark David (Sandia National Laboratories, Albuquerque, NM); Kaiser, Julia N. (Global Product Management, Hilden, Germany); Kozina, Carol L.; Chirica, Gabriela S.

    2012-02-01

    In the response to and recovery from a critical homeland security event involving deliberate or accidental release of biological agents, initial decontamination efforts are necessarily followed by tests for the presence of residual live virus or bacteria. Such 'clearance sampling' should be rapid and accurate, to inform decision makers as they take appropriate action to ensure the safety of the public and of operational personnel. However, the current protocol for clearance sampling is extremely time-intensive and costly, and requires significant amounts of laboratory space and capacity. Detection of residual live virus is particularly problematic and time-consuming, as it requires evaluation of replication potential within a eukaryotic host such as chicken embryos. The intention of this project was to develop a new method for clearance sampling, by leveraging Sandia's expertise in the biological and material sciences in order to create a C. elegans-based foam that could be applied directly to the entire contaminated area for quick and accurate detection of any and all residual live virus by means of a fluorescent signal. Such a novel technology for rapid, on-site detection of live virus would greatly interest the DHS, DoD, and EPA, and hold broad commercial potential, especially with regard to the transportation industry.

  1. The Energy Institute Live Green, Burn Clean

    E-Print Network [OSTI]

    Lee, Dongwon

    The Energy Institute Live Green, Burn Clean: Advancing Engines for Renewable Fuels Live Green, Burn Clean: Advancing Engines for Renewable Fuels André Boehman Professor of Fuel Science and Materials College of Earth and Mineral Sciences The Pennsylvania State University André Boehman Professor of Fuel

  2. s Earth and environment s Living resources

    E-Print Network [OSTI]

    4 s Earth and environment s Living resources s Societies and health s Expertise and consulting of this trend is the acquisition, on a joint proposal from the Earth and Environment department and the Living phenomena so as to improve forecasting of the attendant hazards. The earth's crust: processes and natural

  3. Bioaugmentation of butane-utilizing microorganisms to promote cometabolism of 1,1,1-trichloroethane in groundwater microcosms

    E-Print Network [OSTI]

    Semprini, Lewis

    Bioaugmentation of butane-utilizing microorganisms to promote cometabolism of 1,1,1-trichloroethane. The initial inoculum for bioaugmentation was a butane-utilizing enrichment from the subsurface of the Hanford DOE site. The non-augmented microcosm required 80 days of incubation before butane

  4. WHAT IS COMPOST? Composting refers to the biological decomposition and stabilization of organic materials by microorganisms under

    E-Print Network [OSTI]

    Mukhtar, Saqib

    WHAT IS COMPOST? Composting refers to the biological decomposition and stabilization of organic materials by microorganisms under aerobic (in the presence of oxygen) conditions. During the composting, good quality compost is produced that is biologically stable, relatively uniform in appearance, free

  5. Bioprocessing of lignite coals using reductive microorganisms. Final technical report, September 30, 1988--March 29, 1992

    SciTech Connect (OSTI)

    Crawford, D.L.

    1992-03-29

    In order to convert lignite coals into liquid fuels, gases or chemical feedstock, the macromolecular structure of the coal must be broken down into low molecular weight fractions prior to further modification. Our research focused on this aspect of coal bioprocessing. We isolated, characterized and studied the lignite coal-depolymerizing organisms Streptomyces viridosporus T7A, Pseudomonas sp. DLC-62, unidentified bacterial strain DLC-BB2 and Gram-positive Bacillus megaterium strain DLC-21. In this research we showed that these bacteria are able to solubilize and depolymerize lignite coals using a combination of biological mechanisms including the excretion of coal solublizing basic chemical metabolites and extracellular coal depolymerizing enzymes.

  6. Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1997-12-16

    Bacteria which produce large amounts of cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  7. Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1998-05-26

    Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  8. Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig (Lenoir City, TN)

    1998-01-01

    Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  9. Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig (Lenoir City, TN)

    1997-12-16

    Bacteria which produce large amounts of cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  10. Typical College Student Diet Food Derived Microorganisms and Their Relation to the Human Gastrointestinal Microflora 

    E-Print Network [OSTI]

    Haselhorst, Alexandria J.

    2011-08-08

    plates, the results were truly representative of the actual microbial load within the sample. 8 Homogenization was completed by placing 50 g of food product into a sample bag with a filter insert (VWR, West Chester, Penn.). Some products..., such as the drinks, were shaken prior to being weighed to ensure accurate collection of any bacteria present. The filter bag was filled with 450 mL of sterile Butterfield?s phosphate buffer when solid samples were being processed. The stomacher filter bag...

  11. Author's personal copy Arsenic-resistant bacteria solubilized arsenic in the growth media

    E-Print Network [OSTI]

    Ma, Lena

    Author's personal copy Arsenic-resistant bacteria solubilized arsenic in the growth media bacteria As solubilization Plant growth promotion a b s t r a c t The role of arsenic-resistant bacteria was associated with arsenic-induced plant P uptake. Arsenic resistant bacteria may have potential to enhance

  12. Author's personal copy Morts d'amour: mitochondria are bacteria that

    E-Print Network [OSTI]

    Author's personal copy Letters Morts d'amour: mitochondria are bacteria that sometimes become as bacteria, as recently explored in this journal [1], and this highly heuristic viewpoint should also the views of mitochondria as either bacteria or organelles and suggests that some bacteria transmuted

  13. Spatially-Resolved Analysis of Glycolipids and Metabolites in Living Synechococcus sp. PCC7002 Using Nanospray Desorption Electrospray Ionization

    SciTech Connect (OSTI)

    Lanekoff, Ingela T.; Geydebrekht, Oleg V.; Pinchuk, Grigoriy E.; Konopka, Allan; Laskin, Julia

    2013-04-07

    Microorganisms release a diversity of organic compounds that couple interspecies metabolism, enable communication, or provide benefits to other microbes. Increased knowledge of microbial metabolite production will contribute to understanding of the dynamic microbial world and can potentially lead to new developments in drug discovery, biofuel production, and clinical research. Nanospray desorption electrospray ionization (nano-DESI) is an ambient ionization technique that enables detailed chemical characterization of molecules from a specific location on a surface without special sample pretreatment. Due to its ambient nature, living bacterial colonies growing on agar plates can be rapidly and non-destructively analyzed. We performed spatially resolved nano-DESI analysis of living Synechococcus sp. PCC 7002 colonies on agar plates. We use high resolution mass spectrometry and MS/MS analysis of the living Synechococcus sp. PCC 7002 colonies to detect metabolites and lipids, and confirm their identities. We found that despite the high salt content of the agar (osmolarity ca. 700 mM), nano-DESI analysis enables detailed characterization of metabolites produced by the colony. Using this technique, we identified several glycolipids found on the living colonies and examined the effect of the age of the colony on the chemical gradient of glucosylglycerol secreted onto agar.

  14. Workshop on Magnetotactic Bacteria 9-11 June 2008, Balatonfred, Hungary The role of iron sulfide crystals in magnetotactic bacteria for

    E-Print Network [OSTI]

    Dunin-Borkowski, Rafal E.

    Workshop on Magnetotactic Bacteria 9-11 June 2008, Balatonfüred, Hungary The role of iron sulfide crystals in magnetotactic bacteria for magnetotaxis: A transmission electron microscopy study Takeshi, Denmark Magnetotactic bacteria comprise a number of aquatic species that orient and migrate along

  15. Factors Affecting Biodefluorination of Fluorotelomer Alcohols (FTOHs): Degradative Microorganisms, Transformation Metabolites and Pathways, and Effects of Co-substrates 

    E-Print Network [OSTI]

    Kim, Myung Hee 1982-

    2012-12-11

    similar microbial community structure. This is the first study reporting that pure strains of alkane- and fluoroacetate-degrading bacteria can bio-transform FTOHs via different or preferred transformation pathways to remove multiple –CF2– groups from...

  16. Natural analogue studies of the role of colloids, natural organics and microorganisms on radionuclide transport

    SciTech Connect (OSTI)

    McCarthy, J.F.

    1994-10-01

    Colloids may be important as a geochemical transport mechanism for radionuclides at geological repositories if they are (1) present in the groundwater, (2) stable with respect to both colloidal and chemical stabilities, (3) capable of adsorbing radionuclides, especially if the sorption is irreversible, and (4) mobile in the subsurface. The available evidence from natural analogue and other field studies relevant to these issues is reviewed, as is the potential role of mobile microorganisms ({open_quotes}biocolloids{close_quotes}) on radionuclide migration. Studies have demonstrated that colloids are ubiquitous in groundwater, although colloid concentrations in deep, geochemically stable systems may be too low to affect radionuclide transport. However, even low colloid populations cannot be dismissed as a potential concern because colloids appear to be stable, and many radionuclides that adsorb to colloids are not readily desorbed over long periods. Field studies offer somewhat equivocal evidence concerning colloid mobility and cannot prove or disprove the significance of colloid transport in the far-field environment. Additional research is needed at new sites to properly represent a repository far-field. Performance assessment would benefit from natural analogue studies to examine colloid behavior at sites encompassing a suite of probable groundwater chemistries and that mimic the types of formations selected for radioactive waste repositories.

  17. Computational Chemotaxis in Ants and Bacteria over Dynamic Environments

    E-Print Network [OSTI]

    Ramos, Vitorino; Rosa, A C; Abraham, A

    2007-01-01

    Chemotaxis can be defined as an innate behavioural response by an organism to a directional stimulus, in which bacteria, and other single-cell or multicellular organisms direct their movements according to certain chemicals in their environment. This is important for bacteria to find food (e.g., glucose) by swimming towards the highest concentration of food molecules, or to flee from poisons. Based on self-organized computational approaches and similar stigmergic concepts we derive a novel swarm intelligent algorithm. What strikes from these observations is that both eusocial insects as ant colonies and bacteria have similar natural mechanisms based on stigmergy in order to emerge coherent and sophisticated patterns of global collective behaviour. Keeping in mind the above characteristics we will present a simple model to tackle the collective adaptation of a social swarm based on real ant colony behaviors (SSA algorithm) for tracking extrema in dynamic environments and highly multimodal complex functions des...

  18. Rapid identification of bacteria using an umbelliferone fluorescent assay 

    E-Print Network [OSTI]

    Chamblin, Richard Thomas

    1983-01-01

    . Corgnebacterium seudotuberculosis KF which formed hard, dry colonies; or sr 1 11 ~11' LM h1*h f 4 * pt' lly " t"c colonies), 2 loopfulls of' bacteria per ml were estimated. Estimating 2 loopfulls proved to be an acceptable variation in the technique. Wet.... Quarles ( Co-Chairman of Committee) Charles F. Hall ( ember) L. C. Grumbles ( Member) B. G. Foster Head of Depar t) Ian R. Tiz r August 1983 ABSTRACT Rapid Identification of Bacteria Using an Umbelliferone Fluorescent Assay, (August 1983) Richard...

  19. Colonial Invasions, Colonial Lives History 302

    E-Print Network [OSTI]

    Fletcher, Robin

    1 Colonial Invasions, Colonial Lives History 302 Consider Resources Primary Resources: diaries (scholarly vs popular), theses, the Web... Check subject guide under: Research by Subject History Library Reference collection unless otherwise indicated. Encyclopedia of Cuba: People, History, Culture

  20. campus as living laboratory institutional Sustainability Plan

    E-Print Network [OSTI]

    Calgary, University of

    operations campus as living laboratory diversity campus waste institutional Sustainability Plan eliminate priorities energy research 35%ofwastedivertedSTARSSilver 275 sustainability courses investment 60 accessibility faculty leadership 100+ sustainability research projects over $3.8 million in bursaries Calgary

  1. The Live Room : transducing resonant architectures

    E-Print Network [OSTI]

    Bain, Mark, 1966-

    1998-01-01

    The Live Room is a temporary site specific installation presented in building N 51, room 117 on the MIT campus on May 7, 1998 and concluded on June 10, 1998. Using small acoustic intensifying equipment which mount directly ...

  2. UPC: HNB 100 LIVE Hedco Neurosciences Building

    E-Print Network [OSTI]

    Valero-Cuevas, Francisco

    UPC: HNB 100 ­ LIVE Hedco Neurosciences Building UPC Campus Map/Directions: http://www.usc.edu/about/visit/upc/ HSC: CHP 147 -- Video Conference Center for the Health Professional HSC Campus Map/Directions: http

  3. Creating an inspiring and sustainable living

    E-Print Network [OSTI]

    Langendoen, Koen

    to shape and plan for more sustainable development. Specialisations: · Urban TransformationsCreating an inspiring and sustainable living environment Diploma Master of Science Architecture, Urbanism and Building Sciences Credits 120 ECTS, 24 months Start BSc Bouwkunde graduates can enrol monthly

  4. Dynamical real numbers and living systems

    E-Print Network [OSTI]

    Dhurjati Prasad Datta

    2010-01-11

    Recently uncovered second derivative discontinuous solutions of the simplest linear ordinary differential equation define not only an nonstandard extension of the framework of the ordinary calculus, but also provide a dynamical representation of the ordinary real number system. Every real number can be visualized as a living cell -like structure, endowed with a definite evolutionary arrow. We discuss the relevance of this extended calculus in the study of living systems. We also present an intelligent version of the Newton's first law of motion.

  5. Influence of microorganisms on the oxidation state distribution of multivalent actinides under anoxic conditions

    SciTech Connect (OSTI)

    Reed, Donald Timothy [Los Alamos National Laboratory; Borkowski, Marian [Los Alamos National Laboratory; Lucchini, Jean - Francois [Los Alamos National Laboratory; Ams, David [Los Alamos National Laboratory; Richmann, M. K. [Los Alamos National Laboratory; Khaing, H. [Los Alamos National Laboratory; Swanson, J. S. [Los Alamos National Laboratory

    2010-12-10

    The fate and potential mobility of multivalent actinides in the subsurface is receiving increased attention as the DOE looks to cleanup the many legacy nuclear waste sites and associated subsurface contamination. Plutonium, uranium and neptunium are the near-surface multivalent contaminants of concern and are also key contaminants for the deep geologic disposal of nuclear waste. Their mobility is highly dependent on their redox distribution at their contamination source as well as along their potential migration pathways. This redox distribution is often controlled, especially in the near-surface where organic/inorganic contaminants often coexist, by the direct and indirect effects of microbial activity. Under anoxic conditions, indirect and direct bioreduction mechanisms exist that promote the prevalence of lower-valent species for multivalent actinides. Oxidation-state-specific biosorption is also an important consideration for long-term migration and can influence oxidation state distribution. Results of ongoing studies to explore and establish the oxidation-state specific interactions of soil bacteria (metal reducers and sulfate reducers) as well as halo-tolerant bacteria and Archaea for uranium, neptunium and plutonium will be presented. Enzymatic reduction is a key process in the bioreduction of plutonium and uranium, but co-enzymatic processes predominate in neptunium systems. Strong sorptive interactions can occur for most actinide oxidation states but are likely a factor in the stabilization of lower-valent species when more than one oxidation state can persist under anaerobic microbiologically-active conditions. These results for microbiologically active systems are interpreted in the context of their overall importance in defining the potential migration of multivalent actinides in the subsurface.

  6. 30/04/2009 16:15Quiet Bacteria and Antibiotic Resistance: Scientific American Page 1 of 2http://www.sciam.com/article.cfm?id=bacteria-antibiotic-resistance

    E-Print Network [OSTI]

    West, Stuart

    30/04/2009 16:15Quiet Bacteria and Antibiotic Resistance: Scientific American Page 1 of 2http://www.sciam.com/article.cfm?id=bacteria Bacteria and Antibiotic Resistance Bacteria devoted to growth instead of "quorum sensing" communication could beat antibiotic resistance By Melinda Wenner Despite the rising menace of bacteria--at roughly 19

  7. Biodesulfurization techniques: Application of selected microorganisms for organic sulfur removal from coals. Final report

    SciTech Connect (OSTI)

    Elmore, B.B.

    1993-08-01

    As an alternative to post-combustion desulfurization of coal and pre-combustion desulfurization using physicochemical techniques, the microbial desulfurization of coal may be accomplished through the use of microbial cultures that, in an application of various microbial species, may remove both the pyritic and organic fractions of sulfur found in coal. Organisms have been isolated that readily depyritize coal but often at prohibitively low rates of desulfurization. Microbes have also been isolated that may potentially remove the organic-sulfur fraction present in coal (showing promise when acting on organic sulfur model compounds such as dibenzothiophene). The isolation and study of microorganisms demonstrating a potential for removing organic sulfur from coal has been undertaken in this project. Additionally, the organisms and mechanisms by which coal is microbially depyritized has been investigated. Three cultures were isolated that grew on dibenzothiophene (DBT), a model organic-sulfur compound, as the sole sulfur source. These cultures (UMX3, UMX9, and IGTS8) also grew on coal samples as the sole sulfur source. Numerous techniques for pretreating and ``cotreating`` coal for depyritization were also evaluated for the ability to improve the rate or extent of microbial depyritization. These include prewashing the coal with various solvents and adding surfactants to the culture broth. Using a bituminous coal containing 0.61% (w/w) pyrite washed with organic solvents at low slurry concentrations (2% w/v), the extent of depyritization was increased approximately 25% in two weeks as compared to controls. At slurry concentrations of 20% w/v, a tetrachloroethylene treatment of the coal followed by depyritization with Thiobacillus ferrooxidans increased both the rate and extent of depyritization by approximately 10%.

  8. Modelling of bacteria leaching through porous Graham HORGAN

    E-Print Network [OSTI]

    Molchanov, Ilya

    leaching of bacteria through complex soils, the researcher has to employ numerical methods as the known of statistical stability as the diffusion times vary dramatically between different realisations and soil samples of Mathematics and Mechanics, Moscow State University, 119992, Moscow, Russia. 1 #12;In addition, we have shown

  9. Methods for targetted mutagenesis in gram-positive bacteria

    SciTech Connect (OSTI)

    Yang, Yunfeng

    2014-05-27

    The present invention provides a method of targeted mutagenesis in Gram-positive bacteria. In particular, the present invention provides a method that effectively integrates a suicide integrative vector into a target gene in the chromosome of a Gram-positive bacterium, resulting in inactivation of the target gene.

  10. Method for establishing the presence of salmonella bacteria in eggs

    DOE Patents [OSTI]

    Johnston, Roger G. (Los Alamos, NM); Sinha, Dipen N. (Los Alamos, NM)

    1995-01-01

    Measurement of the acoustical resonances in eggs is shown to provide a rapid, noninvasive technique for establishing the presence of Salmonella bacteria. The technique is also sensitive to yolk puncture, shell cracks, and may be sensitive to other yolk properties and to egg freshness. Remote characterization, potentially useful for characterizing large numbers of eggs, has been demonstrated.

  11. Films of bacteria at interfaces: three stages of behaviour

    E-Print Network [OSTI]

    Liana Vaccari; Daniel Allan; Nima Sharifi-Mood; Aayush Singh; Robert Leheny; Kathleen Stebe

    2015-03-25

    Bacterial attachment to a fluid interface can lead to the formation of a film with physicochemical properties that evolve with time. We study the time evolution of interface (micro)mechanics for interfaces between oil and bacterial suspensions by following the motion of colloidal probes trapped by capillarity to determine the interface microrheology. Initially, active bacteria at and near the interface drive superdiffusive motion of the colloidal probes. Over timescales of minutes, the bacteria form a viscoelastic film which we discuss as a quasi-two-dimensional, active, glassy system. To study late stage mechanics of the film, we use pendant drop elastometry. The films, grown over tens of hours on oil drops, are expanded and compressed by changing the drop volume. For small strains, by modeling the films as 2D Hookean solids, we estimate the film elastic moduli, finding values similar to those reported in the literature for the bacteria themselves. For large strains, the films are highly hysteretic. Finally, from wrinkles formed on highly compressed drops, we estimate film bending energies. The dramatic restructuring of the interface by such robust films has broad implications, e.g. in the study of active colloids, in understanding the community dynamics of bacteria, and in applied settings including bioremediation.

  12. Biodegradation potential of wastewater micropollutants by ammonia-oxidizing bacteria

    E-Print Network [OSTI]

    Chu, Kung-Hui "Bella"

    Available online xxxx Keywords: Triclosan Bisphenol A Ibuprofen Ammonia-oxidizing bacteria Nitrosomonas of three wastewater micropollutants (triclosan, bisphenol A, and ibuprofen) by Nitrosomonas europaea and bisphenol A, but not ibuprofen. The degra- dation was observed only in the absence of allylthiourea (an

  13. Introduction The use of immobilized bacteria for ex situ bioremedi-

    E-Print Network [OSTI]

    Hazen, Terry

    , to immobilize degradative bacteria (Radway et al., 1996). The latter study demonstrated that biomass in a Waring blender. Final biomass concentra- tion was 8­17% (dry weight). Degradation experiments B. cepacia G4 cells were induced by adding phenol at 2 mM for 2 h prior to centrifugation (Shields et al., 1989

  14. Metallomics of two microorganisms relevant to heavy metal bioremediation reveal fundamental differences in metal assimilation and utilization

    SciTech Connect (OSTI)

    Lancaster, Andrew [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Menon, Angeli [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Scott, Israel [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Poole, Farris [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Vaccaro, Brian [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Thorgersen, Michael P [ORNL] [ORNL; Geller, Jil [Lawrence Berkeley National Laboratory (LBNL)] [Lawrence Berkeley National Laboratory (LBNL); Hazen, Terry C [ORNL] [ORNL; Hurt, Jr., Richard Ashley [ORNL; Brown, Steven D [ORNL] [ORNL; Elias, Dwayne A [ORNL] [ORNL; Adams, Michael W. W. [University of Georgia, Athens, GA] [University of Georgia, Athens, GA

    2014-01-01

    Although as many as half of all proteins are thought to require a metal cofactor, the metalloproteomes of microorganisms remain relatively unexplored. Microorganisms from different environments are likely to vary greatly in the metals that they assimilate, not just among the metals with well-characterized roles but also those lacking any known function. Herein we investigated the metal utilization of two microorganisms that were isolated from very similar environments and are of interest because of potential roles in the immobilization of heavy metals, such as uranium and chromium. The metals assimilated and their concentrations in the cytoplasm of Desulfovibrio vulgaris strain Hildenborough (DvH) and Enterobacter cloacae strain Hanford (EcH) varied dramatically, with a larger number of metals present in Enterobacter. For example, a total of 9 and 19 metals were assimilated into their cytoplasmic fractions, respectively, and DvH did not assimilate significant amounts of zinc or copper whereas EcH assimilated both. However, bioinformatic analysis of their genome sequences revealed a comparable number of predicted metalloproteins, 813 in DvH and 953 in EcH. These allowed some rationalization of the types of metal assimilated in some cases (Fe, Cu, Mo, W, V) but not in others (Zn, Nd, Ce, Pr, Dy, Hf and Th). It was also shown that U binds an unknown soluble protein in EcH but this incorporation was the result of extracellular U binding to cytoplasmic components after cell lysis.

  15. Senior Adviser Richard Kauffman to Host Live Chat on Renewable...

    Office of Environmental Management (EM)

    Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and Deployment Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and...

  16. University Teams to Showcase Affordable, Energy Efficient Living...

    Office of Environmental Management (EM)

    University Teams to Showcase Affordable, Energy Efficient Living in U.S. Department of Energy Solar Decathlon 2011 University Teams to Showcase Affordable, Energy Efficient Living...

  17. Siphon-based characterization of liveness and liveness-enforcing supervision

    E-Print Network [OSTI]

    Reveliotis, Spiridon "Spyros"

    1 Siphon-based characterization of liveness and liveness-enforcing supervision for sequential of empty, or more generally, deadly marked siphon. The work presented in this paper seeks to develop ­ and in certain cases, empty ­ siphon. In this capacity, the pre- sented results allow also the extension

  18. INTRODuCTION PrOtectiNg Lives ANd LiveLihOOds

    E-Print Network [OSTI]

    i-3 INTRODuCTION NOAA: PrOtectiNg Lives ANd LiveLihOOds NOAA provides weather, water, and climate safe, efficient, and secure transportation on U.S. waterways. The U.S. Marine Transporta- tion System Management Act, National Estuarine Research Reserves, and National Marine Sanctuaries. NOAA also supports

  19. Magnetic microstructures of chains and clusters of iron oxide and sulfide nanocrystals in bacteria

    E-Print Network [OSTI]

    Dunin-Borkowski, Rafal E.

    Magnetic microstructures of chains and clusters of iron oxide and sulfide nanocrystals in bacteria of intracellular ferrimagnetic magnetite (Fe3O4) and greigite (Fe3S4) nanocrystals inside magnetotactic bacteria

  20. Biofilms as sources of fecal bacteria contamination in the stormwater drainage system in Singapore

    E-Print Network [OSTI]

    Burkhart, Tsung Hwa (Tsung Hwa Sophia)

    2013-01-01

    A study was performed to examine a possible source of fecal bacteria contamination originating from within the stormwater drainage system in Singapore. The extent of fecal bacteria presence in storm drain biofilms was ...

  1. The Association of Virulent Vibrio Spp. Bacteria on Gafftopsail and Hardhead Catfish in Galveston Bay 

    E-Print Network [OSTI]

    Gilbert, Leslie Deanne

    2011-10-21

    Vibrio vulnificus (Vv) and V. parahaemolyticus (Vp) are gram negative, halophilic bacteria that occur naturally in estuarine waters of Galveston Bay. Both bacteria have the potential to cause infections in humans either via consumption or direct...

  2. The calcification of Staphylococcus aureus bacteria : a potential defense mechanism against infections

    E-Print Network [OSTI]

    Arellano, Catherine Krystle

    2010-01-01

    in Serum: A Possible Mechanism for Biomineralization. J.Bacteria: A Potential Defense Mechanism Against Infections Amechanism

  3. Squeezing Out the Hidden Lives of Electrons | Advanced Photon...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Performance of Catalytic Converters Earth's Core Reveals an Inner Weakness How Do Bacteria Repair Damage from the Sun? Science Highlights Archives: 2014 2013 | 2012 | 2011...

  4. Working at Living: The Social Relations of Precarity

    E-Print Network [OSTI]

    Boris, Eileen; Dodson, Leigh

    2013-01-01

    and  depth.  They  considered   working  at  living  through  home  labors,  transnational  and  national  migration,  

  5. Student HouSing BenefitS of Living

    E-Print Network [OSTI]

    Matrajt, Graciela

    Student HouSing #12;BenefitS of Living in Student HouSing · Live with your peers in an exciting with housing costs for eligible students · Onsite parking at housing lots · Living Learning Communities the leadership of these units, students will enjoy the usual perks of living in campus housing, but will also

  6. Chemical biology and bacteria: not simply a matter of life or death

    E-Print Network [OSTI]

    Mekalanos, John

    Chemical biology and bacteria: not simply a matter of life or death Deborah T Hung1,3 and Eric J Rubin2,3 Chemical biological approaches to understanding bacteria have largely been confined and that combining chemical biologic and genetic approaches to studying bacteria brings new power to old problems

  7. Characterization of arsenic-resistant bacteria from the rhizosphere of arsenic hyperaccumulator

    E-Print Network [OSTI]

    Ma, Lena

    Characterization of arsenic-resistant bacteria from the rhizosphere of arsenic hyperaccumulator that these arsenic-resistant bacteria are met- abolically adapted to arsenic-induced osmotic or oxidative stresses to the high arsenic resistance in the bacterial isolates. Key words: arsenic-resistant bacteria, Pteris

  8. Source tracking fecal bacteria in water: a critical review of current methods

    E-Print Network [OSTI]

    Mazumder, Asit

    source tracking; E. coli; Fecal bacteria; Water quality; Ribotyping; Antibiotic resistance analysis (ARAReview Source tracking fecal bacteria in water: a critical review of current methods Cynthia L and techniques are being developed to track sources of bacteria in water and food. Currently

  9. Bloom of resident antibiotic-resistant bacteria in soil following manure fertilization

    E-Print Network [OSTI]

    Handelsman, Jo

    Bloom of resident antibiotic-resistant bacteria in soil following manure fertilization Nikolina­ resistance genes in treated and untreated soil, and indicated that the higher frequency of resistant bacteria of antibiotic- resistant bacteria, antibiotic-resistance genes (collectively known as the "resistome

  10. Combating Drug-Resistant Bacteria: Small Molecule Mimics of Plasmid Incompatibility as Antiplasmid Compounds

    E-Print Network [OSTI]

    Hergenrother, Paul J.

    Combating Drug-Resistant Bacteria: Small Molecule Mimics of Plasmid Incompatibility as Antiplasmid, 2004; E-mail: hergenro@uiuc.edu Multidrug resistant bacteria are now ubiquitous in both hospital to vancomycin.2 Due to this prevalence of drug-resistant bacteria, there is a pressing need for novel classes

  11. Single-cell sequencing provides clues about the host interactions of segmented filamentous bacteria (SFB)

    E-Print Network [OSTI]

    Quake, Stephen R.

    resistance has hindered characterization of these enigmatic bacteria. In the present study, we isolated five bacteria (SFB) Su¨nje J. Pamp,1 Eoghan D. Harrington,1 Stephen R. Quake,2,3,4 David A. Relman,1 94304, USA Segmented filamentous bacteria (SFB) are host-specific intestinal symbionts that comprise

  12. Mathematical Analysis of a model describing the invasion of bacteria in burn wounds #

    E-Print Network [OSTI]

    Eindhoven, Technische Universiteit

    and references). The rapid development of antibiotic resistance among bacteria has added urgency to the taskMathematical Analysis of a model describing the invasion of bacteria in burn wounds # D. Hilhorst arises as a model for host tissue degradation by bacteria and involves a parameter describing

  13. Mathematical Analysis of a model describing the invasion of bacteria in burn wounds

    E-Print Network [OSTI]

    Eindhoven, Technische Universiteit

    and references). The rapid development of antibiotic resistance among bacteria has added urgency to the taskMathematical Analysis of a model describing the invasion of bacteria in burn wounds D. Hilhorst arises as a model for host tissue degradation by bacteria and involves a parameter describing

  14. Physica A 359 (2006) 495524 Seeking the foundations of cognition in bacteria

    E-Print Network [OSTI]

    Jacob, Eshel Ben

    2006-01-01

    Physica A 359 (2006) 495­524 Seeking the foundations of cognition in bacteria: From Schro¨ dinger fundamental requirement of Life. In other words, all organisms, including bacteria, the most primitive that by acting together, bacteria can perform this most elementary cognitive function more efficiently as can

  15. When Bacteria Talk: Time Elapse Communication for Super-Slow Networks

    E-Print Network [OSTI]

    Sivakumar, Raghupathy

    When Bacteria Talk: Time Elapse Communication for Super-Slow Networks Bhuvana Krishnaswamy a Escherichia coli bacteria serving as the communication receiver that a simple modulation like on-off keying- ically bio-inspired) for use in biological applications [3]­[5]. In recent years, bacteria have emerged

  16. Magnetic microstructure of chains and clusters of iron oxide and sulfide nanocrystals in bacteria

    E-Print Network [OSTI]

    Dunin-Borkowski, Rafal E.

    Magnetic microstructure of chains and clusters of iron oxide and sulfide nanocrystals in bacteria M Magnetotactic bacteria contain intracellular, membrane-bound ferrimagnetic nanocrystals (magnetosomes) that have of magnetotactic bacteria provide a natural laboratory in which the magnetic properties of nanometer

  17. BACTERIA TMDL IMPLEMENTATION CONTROL STRATEGIES OF THE SOUTHEAST: RECOMMENDATIONS FOR GEORGIA

    E-Print Network [OSTI]

    Radcliffe, David

    BACTERIA TMDL IMPLEMENTATION CONTROL STRATEGIES OF THE SOUTHEAST: RECOMMENDATIONS FOR GEORGIA implementation plans for bacteria in the state of Georgia. Methods include reviewing relevant literature, policy bacteria TMDL implementation plans; 2) showing control strategies in other states that could be implemented

  18. A simple bioclogging model that accounts for spatial spreading of bacteria

    E-Print Network [OSTI]

    Demaret, Laurent

    A simple bioclogging model that accounts for spatial spreading of bacteria HJ Eberla , L Demaretb is presented that accounts for spatial expansion of the bacterial popu- lation in the soil. The bacteria move harmful environ- mental factors and mechanical washout. Naturally occurring bacteria are a major

  19. Label-free bacteria detection using evanescent mode of a suspended core terahertz fiber

    E-Print Network [OSTI]

    Skorobogatiy, Maksim

    Label-free bacteria detection using evanescent mode of a suspended core terahertz fiber Anna time an E. coli bacteria sensor based on the evanescent field of the fundamental mode of a suspended\\ SKDJHV DQG WKHQ GHVWUR\\HG ZLWK a P-size fragments remaining bound to the fiber surface. The bacteria

  20. Under consideration for publication in J. Fluid Mech. 1 The wiggling trajectories of bacteria

    E-Print Network [OSTI]

    Hyon, YunKyong

    Under consideration for publication in J. Fluid Mech. 1 The wiggling trajectories of bacteria Y U N) Many motile bacteria display wiggling trajectories, which correspond to helical swimming paths and orientations relative to the cell body for peritrichously flagellated bacteria. Modeling the bundle as a rigid

  1. Bacteria Targeted By Human Natural Antibodies Using -Gal Conjugated Receptor-specic Glycopolymers

    E-Print Network [OSTI]

    Chen, Xi

    Bacteria Targeted By Human Natural Antibodies Using -Gal Conjugated Receptor-speci®c Glycopolymers as speci®c ligands for bacterial cells. The binding of the resulting multivalent glycopolymer to bacteria and infectious disease. However, many bacteria undergo genetic changes to eschew the host immune system. In spite

  2. Physical Channel Characterization for Medium-Range Nano-Networks using Flagellated Bacteria

    E-Print Network [OSTI]

    Politècnica de Catalunya, Universitat

    Physical Channel Characterization for Medium-Range Nano-Networks using Flagellated Bacteria Maria-µm): flagellated bacteria. This tech- nique is based on the transport of DNA-encoded information between emit- ters-machines. Keywords: Nano-networks; Molecular Communication; Flagellated Bacteria; DNA Packet; Propagation delay

  3. Bacteria adds to fly lifespan Calgary Herald Calgary, Alta.:Aug 17, 2004. p. A4

    E-Print Network [OSTI]

    Seroude, Laurent

    Bacteria adds to fly lifespan Calgary Herald Calgary, Alta.:Aug 17, 2004. p. A4 Abstract (Article Summary) Exposing the insect drosophila to bacteria at various moments of its notoriously short existence, the researchers found if bacteria were successful in latching onto a host fly within the first four to seven days

  4. Phylogenetic analysis of nitric oxide reductase gene homologues from aerobic ammonia-oxidizing bacteria

    E-Print Network [OSTI]

    Ward, Bess

    -oxidizing bacteria Karen L. Casciotti *,1 , Bess B. Ward Department of Geosciences, Princeton University, Princeton) are climatically important trace gases that are produced by both nitrifying and den- itrifying bacteria-oxidizing bacteria, including Nitrosomonas and Nitrosococcus species (i.e., both b- and c-Proteobacterial ammonia

  5. 788 NATURE PHYSICS | VOL 10 | NOVEMBER 2014 | www.nature.com/naturephysics Sizing up bacteria

    E-Print Network [OSTI]

    Loss, Daniel

    bacteria Chances are you've never seen a bacterium with your naked eye. The largest physical dimension smaller than the width of a human hair. We've all seen bacteria, but only with the aid of a microscope. You may wonder -- why aren't some bacteria a lot bigger? Well, there's a fairly obvious answer

  6. Phosphorus solubilization and plant growth enhancement by arsenic-resistant bacteria

    E-Print Network [OSTI]

    Ma, Lena

    Phosphorus solubilization and plant growth enhancement by arsenic-resistant bacteria Piyasa Ghosh a, University of Florida, Gainesville, FL 32611, United States h i g h l i g h t s Arsenic-resistant bacteria 2015 Accepted 23 March 2015 Handling Editor: J. de Boer Keywords: Arsenic-resistant bacteria FePO4

  7. Surface-Enhanced Infrared Absorption-Reflectance (SEIRA) Microspectroscopy A Chemical/Biological Probe for Bacteria

    E-Print Network [OSTI]

    /Biological Probe for Bacteria Localization in Geologic Materials Hoi-Ying N. Holman, Dale L. Perry, Michael C and the possibility of using the intrinsic endolithic (rock/mineral-inhabiting) bacteria to either detoxify or immobilize the pollutants have stimulated new interests in the exploration of endolithic bacteria

  8. Development of a simple and sensitive fluorimetric method for isolation of coumaphos-hydrolysing bacteria

    E-Print Network [OSTI]

    Hammock, Bruce D.

    -hydrolysing bacteria R.L. Harcourt*, I. Horne, T.D. Sutherland, B.D. Hammock1 , R.J. Russell and J.G. Oakeshott . Incorporation of coumaphos into agar plates allowed the rapid detection of coumaphos-hydrolysing bacteria when tool to screen for bacteria possessing phosphotriesterase activity. INTRODUCTION Organophosphorus (OP

  9. Magnetic Microstructure of Closely-Spaced Ferrimagnetic Crystals in Magnetotactic Bacteria

    E-Print Network [OSTI]

    Dunin-Borkowski, Rafal E.

    Magnetic Microstructure of Closely-Spaced Ferrimagnetic Crystals in Magnetotactic Bacteria R. E of Cambridge, Pembroke Street, Cambridge CB2 3QZ, United Kingdom Magnetotactic bacteria migrate along of magnetite and greigite crystals in air-dried cells of magnetotactic bacteria, with sub-10-nm spatial

  10. Polar features in the flagellar propulsion of E. coli bacteria

    E-Print Network [OSTI]

    Bianchi, S; Lepore, A; Di Leonardo, R

    2015-01-01

    E. coli bacteria swim following a run and tumble pattern. In the run state all flagella join in a single helical bundle that propels the cell body along approximately straight paths. When one or more flagellar motors reverse direction the bundle unwinds and the cell randomizes its orientation. This basic picture represents an idealization of a much more complex dynamical problem. Although it has been shown that bundle formation can occur at either pole of the cell, it is still unclear whether this two run states correspond to asymmetric propulsion features. Using holographic microscopy we record the 3D motions of individual bacteria swimming in optical traps. We find that most cells possess two run states characterised by different propulsion forces, total torque and bundle conformations. We analyse the statistical properties of bundle reversal and compare the hydrodynamic features of forward and backward running states. Our method is naturally multi-particle and opens up the way towards controlled hydrodynam...

  11. Effective run-and-tumble dynamics of bacteria baths

    E-Print Network [OSTI]

    M. Paoluzzi; R. Di Leonardo; L. Angelani

    2013-07-30

    {\\it E. coli} bacteria swim in straight runs interrupted by sudden reorientation events called tumbles. The resulting random walks give rise to density fluctuations that can be derived analytically in the limit of non interacting particles or equivalently of very low concentrations. However, in situations of practical interest, the concentration of bacteria is always large enough to make interactions an important factor. Using molecular dynamics simulations, we study the dynamic structure factor of a model bacterial bath for increasing values of densities. We show that it is possible to reproduce the dynamics of density fluctuations in the system using a free run-and-tumble model with effective fitting parameters. We discuss the dependence of these parameters, e.g., the tumbling rate, tumbling time and self-propulsion velocity, on the density of the bath.

  12. Reduction of trichloroethylene in a model aquifer with methanotrophic bacteria 

    E-Print Network [OSTI]

    Hicks, Duane Dee

    1990-01-01

    REDUCTION OF TRICHLOROETHYLENE IN A MODEL AQUIFER WITH METHANOTROPHIC BACTERIA A Thesis by Duane Dee Hicks Submitted to the Office of Graduate Studies of Texas ARM University in partial fullfillment of the requirements for thc degree... of MASTER OF SCIENCE December 1990 Major Subject: Civil Engineering REDUCTION OF TRICHLOROETHYLENE IN A MODEL AQUIFER WITH METHANOTROPHIC BACTEPslA A Thesis by Duane Dec Hicks Approved as to style and content by Bill Batchclor (Chair of Committee...

  13. Petroleum hydrocarbon-degrading bacteria in the Galveston Bay system 

    E-Print Network [OSTI]

    Schropp, Steven James

    1979-01-01

    activities and shipping may all release petroleum hydrocarbons into the marine environment (83), which are potentially detrimental to the estuarine system. Estuaries have been found to be one of the most active types of water in terms of microbial... bacteria to oil inpiit might be used as an indicator or petroleuin pollution (6, 43, 44). To evaluate the effects of ) vllutant hydrocarbons ozi the bacterial populations of estuarine systems, information on the distribution and abundance...

  14. Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing BacteriaConnect Collider Testspolycarbonate and

  15. Brisbane 2011: Living with floods and

    E-Print Network [OSTI]

    Boynton, Walter R.

    Brisbane 2011: Living with floods and dancing with dugongs 07 July 2011 Customs House University of Queensland Bill Dennison #12;Outline ·! Queensland floods = societal learning moment ·! Global lessons about flood responses ·! Conservation icons and charismatic ecosystems ·! Sustainability models for the future

  16. Living up to standards Margaret King

    E-Print Network [OSTI]

    to record my thanks to Nigel Bevan, technical editor of the ISO standards discussed for much interestingLiving up to standards Margaret King TIM/ISSCO ETI University of Geneva Margaret reusable" by arguing that a set of ISO standards developed for the evaluation of software in general

  17. Quality health plans & benefits Healthier living

    E-Print Network [OSTI]

    Zhigilei, Leonid V.

    Quality health plans & benefits Healthier living Financial well-being Intelligent solutions Do the Math! 2015-2016 University of Virginia Student Health Insurance Plan www.aetnastudenthealth.com 15.03.491.1 #12;A Student Health Insurance Plan may be the best alternative and here's why: A student health

  18. Live Paint: Painting with Procedural Multiscale Textures

    E-Print Network [OSTI]

    Live Paint: Painting with Procedural Multiscale Textures Ken Perlin Media Research Laboratory´atica Pura e Aplicada Abstract We present actively procedural multiresolution paint textures. Tex- ture drawing tool that can be used in a multires- olution paint system. They provide a mechanism to generate

  19. Live Paint: Painting with Procedural Multiscale Textures

    E-Print Network [OSTI]

    de Figueiredo, Luiz Henrique

    Live Paint: Painting with Procedural Multiscale Textures (Expanded Version) Ken Perlin Media -- Instituto de Matemâ??atica Pura e Aplicada Abstract We present actively procedural multiresolution paint constitute a powerful drawing tool that can be used in a multires­ olution paint system. They provide

  20. Long-lived tendrils viscous entrainment

    E-Print Network [OSTI]

    Gardel, Margaret

    Long-lived tendrils in viscous entrainment Wendy W. Zhang Laura E. Schmidt* Physics & James Franck entrained by warm upwards flow in thermal boundary layer ·Entrainment stabilizes upwards flow Kumagai et al-layer liquid entrained as steady spout Cohen & Nagel 2002 Phys. Rev. Lett. Cohen 2004 Phys. Rev. E Kleine

  1. Degradation of Staphylococcus aureus bacteria by neutral oxygen atoms

    SciTech Connect (OSTI)

    Cvelbar, U.; Mozetic, M. [Jozef Stefan Institute, Jamova cesta 39, SI-1000 Ljubljana (Slovenia); Hauptman, N.; Klanjsek-Gunde, M. [National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana (Slovenia)

    2009-11-15

    The degradation of Staphylococcus aureus bacteria during treatment with neutral oxygen atoms was monitored by scanning electron microscopy. Experiments were performed in an afterglow chamber made from borosilicate glass. The source of oxygen atoms was remote inductively coupled radiofrequency oxygen plasma. The density of atoms at the samples was 8x10{sup 20} m{sup -3}. The treatment was performed at room temperature. The first effect was the removal of dried capsule. Capsule on exposed parts of bacteria was removed after receiving the dose of 6x10{sup 23} at./m{sup 2}, while the parts of capsule filling the gaps between bacteria were removed after receiving the dose of 2.4x10{sup 24} m{sup -2}. After removing the capsule, degradation continued as etching of bacterial cell wall. The etching was rather nonuniform as holes with diameter of several 10 nm were observed. The cell wall was removed after receiving the dose of about 7x10{sup 24} m{sup -2}. The etching probabilities were about 2x10{sup -5} for the capsule and 2x10{sup -6} for the cell wall. The results were explained by different compositions of capsule and the cell wall.

  2. Differential Dynamic Microscopy to characterize Brownian motion and bacteria motility

    E-Print Network [OSTI]

    David Germain; Mathieu Leocmach; Thomas Gibaud

    2015-11-03

    We have developed a lab work module where we teach undergraduate students how to quantify the dynamics of a suspension of microscopic particles, measuring and analyzing the motion of those particles at the individual level or as a group. Differential Dynamic Microscopy (DDM) is a relatively recent technique that precisely does that and constitutes an alternative method to more classical techniques such as dynamics light scattering (DLS) or video particle tracking (VPT). DDM consists in imaging a particle dispersion with a standard light microscope and a camera. The image analysis requires the students to code and relies on digital Fourier transform to obtain the intermediate scattering function, an autocorrelation function that characterizes the dynamics of the dispersion. We first illustrate DDM on the textbook case of colloids where we measure the diffusion coefficient. Then we show that DDM is a pertinent tool to characterize biologic systems such as motile bacteria i.e.bacteria that can self propel, where we not only determine the diffusion coefficient but also the velocity and the fraction of motile bacteria. Finally, so that our paper can be used as a tutorial to the DDM technique, we have joined to this article movies of the colloidal and bacterial suspensions and the DDM algorithm in both Matlab and Python to analyze the movies.

  3. Conducting Nanosponge Electroporation for Affordable and High-Efficiency Disinfection of Bacteria and Viruses in Water

    E-Print Network [OSTI]

    Cui, Yi

    byproducts formation, energy and time intensiveness, and pathogen recovery. Here, we report an innovative more than 3 logs (99.9%) of microorganism removal, voltages as high as 103 to 105 volts are required

  4. Emergent patterns of diversity and dynamics in natural populations of planktonic Vibrio bacteria

    E-Print Network [OSTI]

    Thompson, Janelle Renée, 1976-

    2005-01-01

    Despite the importance of microorganisms for global and engineering processes, currently lacking is a theoretical framework to describe how the structure of a microbial assemblage translates an environmental condition into ...

  5. The future is now: single-cell genomics of bacteria and archaea

    E-Print Network [OSTI]

    Blainey, Paul C.

    Interest in the expanding catalog of uncultivated microorganisms, increasing recognition of heterogeneity among seemingly similar cells, and technological advances in whole-genome amplification and single-cell manipulation ...

  6. Fission barriers and half-lives

    SciTech Connect (OSTI)

    Moeller, P.; Nix, J.R.; Swiatecki, W.J.

    1989-01-01

    We briefly review the development of theoretical models for the calculation of fission barriers and half-lives. We focus on how results of actual calculations in a unified macroscopic-microscopic approach provide an interpretation of the mechanisms behind some of the large number of phenomena observed in fission. As instructive examples we choose studies of the rapidly varying fission properties of elements at the end of the periodic system. 31 refs., 10 figs.

  7. Trapping and Assembly of Living Colloids at Water/Water Interfaces

    E-Print Network [OSTI]

    Sarah D. Hann; Mark Goulian; Daeyeon Lee; Kathleen J. Stebe

    2014-12-11

    We study the assembly of colloids in a two phase water-water system that provides an environment that can sustain bacteria, providing a new structure with rich potential to confine and structure living colloids. The water-water system, formed via phase separation of a casein and xanthan mixture, forms a 3-D structure of coexisting casein-rich and xanthan-rich phases. Fluorescent labelling and confocal microscopy reveal the attachment of these living colloids, including Escherichia coli and Pseudomonas aeruginosa, at the interface between the two phases. Inert colloids also become trapped at the interfaces, suggesting that the observed attachment can be attributed to capillarity. Over time, these structures coarsen and eventually degrade, illustrating the dynamic nature of these systems. This system lays the foundation for future studies of the interplay of physicochemical properties of the fluid interfaces and bulk phases and microbial responses they provoke to induce complex spatial organization, to study species which occupy distinct niches, and to optimize efficient microbial cross-feeding or protection from competitors.

  8. Living Comfortably: A Consumer's Guide to Home Energy Upgrades...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Living Comfortably: A Consumer's Guide to Home Energy Upgrades Living Comfortably: A Consumer's Guide to Home Energy Upgrades March 7, 2013 - 3:15pm Addthis A weatherization worker...

  9. Solar Decathlon Team Leading the Way Toward Sustainable Living...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Solar Decathlon Team Leading the Way Toward Sustainable Living, Even in the Wake of Disasters Solar Decathlon Team Leading the Way Toward Sustainable Living, Even in the Wake of...

  10. Acting Biomass Program Manager Dr. Valerie Reed to Host Live...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Acting Biomass Program Manager Dr. Valerie Reed to Host Live Twitter Q&A on Advanced Biofuels Acting Biomass Program Manager Dr. Valerie Reed to Host Live Twitter Q&A on Advanced...

  11. Polar features in the flagellar propulsion of E. coli bacteria

    E-Print Network [OSTI]

    S. Bianchi; F. Saglimbeni; A. Lepore; R. Di Leonardo

    2015-06-30

    E. coli bacteria swim following a run and tumble pattern. In the run state all flagella join in a single helical bundle that propels the cell body along approximately straight paths. When one or more flagellar motors reverse direction the bundle unwinds and the cell randomizes its orientation. This basic picture represents an idealization of a much more complex dynamical problem. Although it has been shown that bundle formation can occur at either pole of the cell, it is still unclear whether this two run states correspond to asymmetric propulsion features. Using holographic microscopy we record the 3D motions of individual bacteria swimming in optical traps. We find that most cells possess two run states characterised by different propulsion forces, total torque and bundle conformations. We analyse the statistical properties of bundle reversal and compare the hydrodynamic features of forward and backward running states. Our method is naturally multi-particle and opens up the way towards controlled hydrodynamic studies of interacting swimming cells.

  12. Morgantown Slightly Exceeds National Average for Cost of Living

    E-Print Network [OSTI]

    Mohaghegh, Shahab

    (an index value of 100 reflects the national average). The index expresses the cost of living, health care, and miscellaneous goods and services. The index is designed to reflect the cost of living Relative to National Average by Category In Figure 2, we illustrate how the cost of living index has

  13. Hierarchical classification with reject option for live fish recognition

    E-Print Network [OSTI]

    Fisher, Bob

    Hierarchical classification with reject option for live fish recognition Phoenix X. Huang@inf.ed.ac.uk Robert B. Fisher University of Edinburgh rbf@inf.ed.ac.uk Abstract A live fish recognition system. We present a novel Balance-Enforced Optimized Tree with Reject op- tion (BEOTR) for live fish

  14. Proton radioactivity half lives with Skyrme interactions

    E-Print Network [OSTI]

    T. R. Routray; Abhishek Mishra; S. K. Tripathy; B. Behera; D. N. Basu

    2012-05-31

    The potential barrier impeding the spontaneous emission of protons in the proton radioactive nuclei is calculated as the sum of nuclear, Coulomb and centrifugal contributions. The nuclear part of the proton-nucleus interaction potential is obtained in the energy density formalism using Skyrme effective interaction that results into a simple algebraic expression. The half-lives of the proton emitters are calculated for the different Skyrme sets within the improved WKB framework. The results are found to be in reasonable agreement with the earlier results obtained for more complicated calculations involving finite range interactions.

  15. Bursts of Active Transport in Living Cells

    E-Print Network [OSTI]

    Bo Wang; James Kuo; Steve Granick

    2013-07-07

    We scrutinize the temporally-resolved speed of active cargo transport in living cells, and show intermittent bursting motions. These nonlinear fluctuations follow a scaling law over several decades of time and space, the statistical regularities displaying a time-averaged shape that we interpret to reflect stress buildup followed by rapid release. The power law of scaling is the same as seen in driven jammed colloids, granular, and magnetic systems. The implied regulation of active transport with environmental obstruction extends the classical notion of molecular crowding.

  16. Safety assessment of outdoor live fire range

    SciTech Connect (OSTI)

    1989-05-01

    The following Safety Assessment (SA) pertains to the outdoor live fire range facility (LFR). The purpose of this facility is to supplement the indoor LFR. In particular it provides capacity for exercises that would be inappropriate on the indoor range. This SA examines the risks that are attendant to the training on the outdoor LFR. The outdoor LFR used by EG&G Mound is privately owned. It is identified as the Miami Valley Shooting Grounds. Mondays are leased for the exclusive use of EG&G Mound.

  17. Solar Living Institute | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page| Open Energy Information Serbia-Enhancing Capacity forSilicium deEnergy InformationDepotGreen TechnologyLiving

  18. Living Walls | OpenEI Community

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar2-0057-EAInvervarLeeds, UnitedLibertyLite On TechnologyCornLiuzhouLiving Walls

  19. Women @ Energy: Kelly Lively | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of Natural GasAdjustmentsShirleyEnergyThe U.S.Lacledeutilities.Energy ThefullAssociateJenniferCarradoKelly Lively has

  20. Live pathogens: rapid detection technique developed

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformationJesse BergkampCenter (LMI-EFRC) ProximityCenterLee FacultyadivisionLive

  1. Process for generation of hydrogen gas from various feedstocks using thermophilic bacteria

    DOE Patents [OSTI]

    Ooteghem, Suellen Van (Morgantown, WV)

    2005-09-13

    A method for producing hydrogen gas is provided comprising selecting a bacteria from the Order Thermotogales, subjecting the bacteria to a feedstock and to a suitable growth environment having an oxygen concentration below the oxygen concentration of water in equilibrium with air; and maintaining the environment at a predetermined pH and at a temperature of at least approximately 45.degree. C. for a time sufficient to allow the bacteria to metabolize the feedstock.

  2. Purple Bacteria Develops Its Own Form of Sunscreen | U.S. DOE...

    Office of Science (SC) Website

    Home Centers Research Science Highlights Highlight Archives News & Events Publications History Contact BES Home 05.03.12 Purple Bacteria Develops Its Own Form of "Sunscreen" Print...

  3. Contribution of Iron-Reducing Bacteria to Mercury Methylation in Marine Sediments

    E-Print Network [OSTI]

    Fleming, Emily J.; Nelson, D C

    2006-01-01

    in continental margin sediments off central Chile. Limnologyof microbial iron reduction in sediments of the Baltic-Northreducing bacteria from sediments of an acid stressed lake.

  4. Evidence of radicals created by plasma in bacteria in water

    SciTech Connect (OSTI)

    Lee, Chae Bok; Na, Young Ho; Hong, Tae-Eun; Choi, Eun Ha; Uhm, Han S.; Baik, Ku Youn E-mail: gckwon@kw.ac.kr; Kwon, Gichung E-mail: gckwon@kw.ac.kr

    2014-08-18

    Heavy water (D{sub 2}O) was introduced into a non-thermal plasma-jet (NTPJ) device to generate deuterium monoxide (OD) radicals at room temperature. Owing to the similar reactivity and low prevalence of deuterium in nature, OD radicals can be utilized to visualize the OH radical interactions with water and living cells. Escherichia coli in water were treated with OD radicals, and D atom incorporation into cells was visualized using time-of-flight SIMS and Nano-SIMS. The results show that D atoms from NTPJ reach the cytoplasm of E. coli in H{sub 2}O, indicating the usefulness of this OD-tracking method for the study of radical interactions with living cells.

  5. Mechanism for collective cell alignment in Myxococcus xanthus bacteria

    E-Print Network [OSTI]

    Balagam, Rajesh

    2015-01-01

    M. xanthus cells self-organize into clusters, aligned cell groups, at various stages of its lifecycle. Formation of these clusters is crucial for complex dynamic multi-cellular behavior of these bacteria. However the mechanism underlying the cell alignment and clustering is not fully understood. Motivated by studies of clustering in self-propelled rods, we hypothesized that M. xanthus cells can align and form clusters through pure mechanical interactions among cells and between cells and substrate. We test this hypothesis using an agent-based simulation framework where each agent is based on biophysical model of individual M. xanthus cell. We show that model agents, under realistic cell flexibility values, can align and form cell clusters but only when periodic reversals of cell directions are suppressed. However, by extending our model to introduce observed ability of cells to lay and follow slime trails, we show that effective trail following leads to clusters in reversing cells. Furthermore, we conclude th...

  6. Bioaugmentation with butane-utilizing microorganisms to promote in situ cometabolic treatment of 1,1,1-trichloroethane and 1,1-dichloroethene

    E-Print Network [OSTI]

    Semprini, Lewis

    Bioaugmentation with butane-utilizing microorganisms to promote in situ cometabolic treatment of 1) through bioaugmentation with a butane enrichment culture containing predominantly two Rhodococcus sp of butane and dissolved oxygen and or hydrogen peroxide as sources of dissolved oxygen, about 70% removal

  7. Marine microorganisms dominate the ocean ecosystem and sustain planetary habitability. The scientists and educators on the C-MORE Team --women and

    E-Print Network [OSTI]

    Marine microorganisms dominate the ocean ecosystem and sustain planetary habitability to a unique partnership aimed at exploring and understanding how the marine microbial world is structured`i Partnership #12;Marine Microbiology Initiative VIA SEA MAIL SCHOOL OF OCEAN AND EARTH SCIENCE AND TECHNOLOGY U

  8. Detergent composition comprising a cellulase containing cell-free fermentate produced from microorganism ATCC 55702 or mutant thereof

    DOE Patents [OSTI]

    Dees, H.C.

    1998-07-14

    Bacteria which produce large amounts of a cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  9. Detergent composition comprising a cellulase containing cell-free fermentate produced from microorganism ATCC 55702 or mutant thereof

    DOE Patents [OSTI]

    Dees, H. Craig (Lenoir City, TN)

    1998-01-01

    Bacteria which produce large amounts of a cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  10. Meso-scale turbulence in living fluids

    E-Print Network [OSTI]

    Wensink, Henricus H; Heidenreich, Sebastian; Drescher, Knut; Goldstein, Raymond E; Löwen, Hartmut; Yeomans, Julia M

    2012-01-01

    Turbulence is ubiquitous, from oceanic currents to small-scale biological and quantum systems. Self-sustained turbulent motion in microbial suspensions presents an intriguing example of collective dynamical behavior amongst the simplest forms of life, and is important for fluid mixing and molecular transport on the microscale. The mathematical characterization of turbulence phenomena in active non-equilibrium fluids proves even more difficult than for conventional liquids or gases. It is not known which features of turbulent phases in living matter are universal or system-specific, or which generalizations of the Navier-Stokes equations are able to describe them adequately. Here, we combine experiments, particle simulations, and continuum theory to identify the statistical properties of self-sustained meso-scale turbulence in active systems. To study how dimensionality and boundary conditions affect collective bacterial dynamics, we measured energy spectra and structure functions in dense Bacillus subtilis su...

  11. Living and Working in the Freezer

    SciTech Connect (OSTI)

    Hill, Victoria (Dept of Ocean, Earth, and Atmospheric Sciences, Old Dominion Unversity) [Dept of Ocean, Earth, and Atmospheric Sciences, Old Dominion Unversity

    2012-02-07

    Very little data of any kind exists from the early spring in the Arctic. The reason? It's extremely cold and that makes it difficult to survive, let alone conduct science. From March through the end of April, 2011, scientists from around the world braved temperatures of -48?C in the high Canadian Arctic in the name of science. At the Catlin Arctic Survey's floating 'Ice Base' off Ellef Ringnes Island, Dr. Victoria Hill was investigating how organic material in fresh water near the surface of the ocean may be trapping heat from the sun, causing the upper ocean layers to warm. This is a very new area of research and this mechanism represents a key uncertainty in accurate modeling of ice thickness and upper ocean heat content. In this presentation Dr. Hill will talk about living and working at the ice base and discuss preliminary data from the expedition.

  12. In vitro conjugal transfer of tetracycline resistance from Lactobacillus isolates to other Gram-positive bacteria

    E-Print Network [OSTI]

    Gent, Universiteit

    In vitro conjugal transfer of tetracycline resistance from Lactobacillus isolates to other Gram-positive bacteria Dirk Gevers a;Ã , Geert Huys a , Jean Swings a;b a Laboratory of Microbiology, Faculty of Sciences, Ghent University, B-9000 Ghent, Belgium b BCCM1/LMG Bacteria Collection, Faculty of Sciences, Ghent

  13. Spatial arrangements and magnetic properties of oxide and sulfide magnetosomes in magnetotactic bacteria

    E-Print Network [OSTI]

    Dunin-Borkowski, Rafal E.

    bacteria M. Pósfai1 , T. Kasama2,3 , E.T. Simpson3 , R.K.K. Chong3 , I. Kósa1 , A. Scheffel4 , D. Schüler4 of intracellular ferrimagnetic magnetite (Fe3O4) and greigite (Fe3S4) crystals in magnetotactic bacteria

  14. Clay enhancement of methane, low molecular weight hydrocarbon and halocarbon conversion by methanotrophic bacteria

    DOE Patents [OSTI]

    Apel, William A.; Dugan, Patrick R.

    1995-04-04

    An apparatus and method for increasing the rate of oxidation of toxic vapors by methanotrophic bacteria. The toxic vapors of interest are methane and trichloroethylene. The apparatus includes a gas phase bioreactor within a closed loop pumping system or a single pass system. The methanotrophic bacteria include Methylomonas methanica, Methylosinus trichosporium, and uncharacterized environmental enrichments.

  15. Clay enhancement of methane, low molecular weight hydrocarbon and halocarbon conversion by methanotrophic bacteria

    DOE Patents [OSTI]

    Apel, William A. (Idaho Falls, ID); Dugan, Patrick R. (Idaho Falls, ID)

    1995-01-01

    An apparatus and method for increasing the rate of oxidation of toxic vapors by methanotrophic bacteria. The toxic vapors of interest are methane and trichloroethylene. The apparatus includes a gas phase bioreactor within a closed loop pumping system or a single pass system. The methanotrophic bacteria include Methylomonas methanica, Methylosinus trichosporium, and uncharacterized environmental enrichments.

  16. Microbial Grazers Lab Objective: Measure the rate at which bacteria are consumed by predators.

    E-Print Network [OSTI]

    Vallino, Joseph J.

    Microbial Grazers Lab Objective: Measure the rate at which bacteria are consumed by predators. Overview ·Size based food webs ·Microbial loop concepts ·Bacterial predators ·Methods to assess microbial bacteria were know to exist, they were not thought to be significant consumers of carbon or energy. #12

  17. Novel Methane, Ethane, and Propane Oxidizing Bacteria at Marine Hydrocarbon Seeps Identified by Stable Isotope Probing

    E-Print Network [OSTI]

    Sessions, Alex L.

    Novel Methane, Ethane, and Propane Oxidizing Bacteria at Marine Hydrocarbon Seeps Identified by Stable Isotope Probing Running Title: Novel Methane, Ethane, and Propane Oxidizing Bacteria Section incubating sediment with 13 C-labeled methane, ethane, or propane, we5 confirmed the incorporation of 13 C

  18. Aerobic uranium (VI) bioprecipitation by metal-resistant bacteria isolated from radionuclide-

    E-Print Network [OSTI]

    Skolnick, Jeff

    Aerobic uranium (VI) bioprecipitation by metal-resistant bacteria isolated from radionuclide uranium [U(VI)] mediated by the intrinsic phosphatase acti- vities of naturally occurring bacteria such as uranium (U), technetium (Tc) and other toxic metals [e.g. cadmium (Cd), lead (Pb), chromium (Cr

  19. Slow urban living apartments : transformation of five story walk-up apartments in Seoul

    E-Print Network [OSTI]

    Lim, Yihyun

    2014-01-01

    Experiential living is the new trend for future living. Whether it is through living in micro spaces, flexible units, mixed-use developments, practicing urban farming, or sharing lifestyles, these different trends of living ...

  20. Live Plant Sample c/o Rob Richardson

    E-Print Network [OSTI]

    Krings, Alexander

    and degrade. 6. Next day or priority mail is recommended for shipping fresh samples. Be sure package is labeled with "Live Plant" #12;

  1. Brownsville Public Utilities Board- Green Living Residential Rebate Program

    Broader source: Energy.gov [DOE]

     Brownsville Public Utilities Board offers residential customers rebates for installation of energy efficient measures. Through the Green Living Rebate program, customers can apply for rebates for...

  2. Live, Audio-Visual Communication Systems for Distance Learning

    E-Print Network [OSTI]

    Finkelstein, Anthony

    , WC1H 0AP, U.K. tel: (44) (0)71 387 7050 ext. 5315 fax: (44) (0)71 580 1100 #12;Submission Live, Audio

  3. Spontaneous fission half-lives for ground state nuclides

    SciTech Connect (OSTI)

    Holden, N.E. [Brookhaven National Lab., Upton, NY (United States); Hoffman, D.C. [Lawrence Berkeley Lab., CA (United States)

    1993-08-01

    Measurements of the spontaneous fission half-lives of nuclides of elements Z = 90 to 108 have been compiled and evaluated. Recommended values are presented.

  4. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    protein phosphorylation inside living mammalian cells, enabling them to follow cellular chemical changes in real time, without bias. Advancing Medicine with Infrared Light...

  5. An ultra-sensitive microfluidic immunoassay using living radical...

    Office of Scientific and Technical Information (OSTI)

    microfluidic immunoassay using living radical polymerization and porous polymer monoliths. Citation Details In-Document Search Title: An ultra-sensitive microfluidic...

  6. Virtual Institute of Microbial Stress and Survival: Deduction of Stress Response Pathways in Metal and Radionuclide Reducing Microorganisms

    SciTech Connect (OSTI)

    2004-04-17

    The projects application goals are to: (1) To understand bacterial stress-response to the unique stressors in metal/radionuclide contamination sites; (2) To turn this understanding into a quantitative, data-driven model for exploring policies for natural and biostimulatory bioremediation; (3) To implement proposed policies in the field and compare results to model predictions; and (4) Close the experimental/computation cycle by using discrepancies between models and predictions to drive new measurements and construction of new models. The projects science goals are to: (1) Compare physiological and molecular response of three target microorganisms to environmental perturbation; (2) Deduce the underlying regulatory pathways that control these responses through analysis of phenotype, functional genomic, and molecular interaction data; (3) Use differences in the cellular responses among the target organisms to understand niche specific adaptations of the stress and metal reduction pathways; (4) From this analysis derive an understanding of the mechanisms of pathway evolution in the environment; and (5) Ultimately, derive dynamical models for the control of these pathways to predict how natural stimulation can optimize growth and metal reduction efficiency at field sites.

  7. A solution to the subdiffusion-efficiency paradox: Inactive states enhance reaction efficiency at subdiffusion conditions in living cells

    E-Print Network [OSTI]

    Leila Esmaeili Sereshki; Michael A. Lomholt; Ralf Metzler

    2012-03-08

    Macromolecular crowding in living biological cells effects subdiffusion of larger biomolecules such as proteins and enzymes. Mimicking this subdiffusion in terms of random walks on a critical percolation cluster, we here present a case study of EcoRV restriction enzymes involved in vital cellular defence. We show that due to its so far elusive propensity to an inactive state the enzyme avoids non-specific binding and remains well-distributed in the bulk cytoplasm of the cell. Despite the reduced volume exploration capability of subdiffusion processes, this mechanism guarantees a high efficiency of the enzyme. By variation of the non-specific binding constant and the bond occupation probability on the percolation network, we demonstrate that reduced non-specific binding are beneficial for efficient subdiffusive enzyme activity even in relatively small bacteria cells. Our results corroborate a more local picture of cellular regulation.

  8. 16/11/2007 08:48The bacteria can cheat on their mates (11/16/2007) Page 1 of 3http://www.geneticarchaeology.com/Research/The_bacteria_can_cheat_on_their_mates.asp

    E-Print Network [OSTI]

    West, Stuart

    16/11/2007 08:48The bacteria can cheat on their mates (11/16/2007) Page 1 of 3http://www.geneticarchaeology.com/Research/The_bacteria_can_cheat_on_their_mates.asp www.Affinity- CareTraining.co.uk Ads Dental Qualified & Latest Technol www.StyleSmileClinics.co.uk The bacteria can cheat on their mates (11

  9. 11/17/2007 05:21 PMCooperating bacteria are vulnerable to slackers Not Exactly Rocket Science Page 1 of 5http://notexactlyrocketscience.wordpress.com/2007/11/15/cooperating-bacteria-are-vulnerable-to-slackers/#comment-8042

    E-Print Network [OSTI]

    West, Stuart

    11/17/2007 05:21 PMCooperating bacteria are vulnerable to slackers « Not Exactly Rocket Science Page 1 of 5http://notexactlyrocketscience.wordpress.com/2007/11/15/cooperating-bacteria-NonCommercial- ShareAlike 2.5 License. Carnivals #12;11/17/2007 05:21 PMCooperating bacteria are vulnerable to slackers

  10. Simultaneous regulation of cell size and chromosome replication in bacteria

    E-Print Network [OSTI]

    Po-Yi Ho; Ariel Amir

    2015-07-24

    Bacteria are able to maintain a narrow distribution of cell sizes by regulating the timing of cell divisions. In rich nutrient conditions, cells divide much faster than their chromosomes replicate. This implies that cells maintain multiple rounds of chromosome replication per cell division by regulating the timing of chromosome replications. Here, we show that both cell size and chromosome replication may be simultaneously regulated by the long-standing initiator accumulation strategy. The strategy proposes that initiators are produced in proportion to the volume increase and is accumulated at each origin of replication, and chromosome replication is initiated when a critical amount per origin has accumulated. We show that this model maps to the incremental model of size control, which was previously shown to reproduce experimentally observed correlations between various events in the cell cycle and explains the exponential dependence of cell size on the growth rate of the cell. Furthermore, we show that this model also leads to the efficient regulation of the timing of initiation and the number of origins consistent with existing experimental results.

  11. Fermentation of aqueous plant seed extracts by lactic acid bacteria

    SciTech Connect (OSTI)

    Schafner, D.W.; Beuchat, R.L.

    1986-05-01

    The effects of lactic acid bacterial fermentation on chemical and physical changes in aqueous extracts of cowpea (Vigna unguiculata), peanut (Arachis hypogea), soybean (Glycine max), and sorghum (Sorghum vulgare) were studied. The bacteria investigated were Lactobacillus helveticus, L. delbrueckii, L. casei, L. bulgaricus, L. acidophilus, and Streptococcus thermophilus. Organisms were inoculated individually into all of the seed extracts; L. bulgaricus and S. thermophilus were also evaluated together as inocula for fermenting the legume extracts. During fermentation, bacterial population and changes in titratable acidity, pH, viscosity, and color were measured over a 72 h period at 37 degrees C. Maximum bacterial populations, titratable acidity, pH, and viscosity varied depending upon the type of extract and bacterial strain. The maximum population of each organism was influenced by fermentable carbohydrates, which, in turn, influenced acid production and change in pH. Change in viscosity was correlated with the amount of protein and titratable acidity of products. Color was affected by pasteurization treatment and fermentation as well as the source of extract. In the extracts inoculated simultaneously with L. bulgaricus and S. thermophilus, a synergistic effect resulted in increased bacterial populations, titratable acidity, and viscosity, and decreased pH in all the legume extracts when compared to the extracts fermented with either of these organisms individually. Fermented extracts offer potential as substitutes for cultured dairy products. 24 references.

  12. Why we live in 3 Dimensions

    E-Print Network [OSTI]

    Carlos Castro; Alex Granik; M. S. El Naschie

    2000-08-18

    A Cantorian fractal spacetime, a family member of von Neumann's noncommutative geometry is introduced as a geometry underlying a new relativity theory which is similar to the relation between general relativity and Riemannian geometry. Based on this model and the new relativity theory an ensemble distribution of all the dimensions of quantum spacetime is derived with the help of Fermat grand theorem. The calculated average dimension is very close to the value of $4+\\phi^3 $ (where $\\phi$ is the golden mean) obtained by El Naschie on the basis of a different approach. It is shown that within the framework of the new relativity the cosmological constant problem is nonexistent, since the Universe self-organizes and self-tunes according to the renormalization group (RG) flow with respect to a local scaling microscopic arrow of time. This implies that the world emerged as a result of a non-equilibrium process of self-organized critical phenomena launched by vacuum fluctuations in Cantorian fractal spacetime $\\cal E^{\\infty}$. It is shown that we are living in a metastable vacuum and are moving towards a fixed point ($ D$ = 4+$\\phi^3$) of the RG. After reaching this point, a new phase transition will drive the universe to a quasi-crystal phase of the lower average dimension of $\\phi^3$.

  13. Long Lived Fourth Generation and the Higgs

    E-Print Network [OSTI]

    Wai-Yee Keung; Pedro Schwaller

    2011-05-31

    A chiral fourth generation is a simple and well motivated extension of the standard model, and has important consequences for Higgs phenomenology. Here we consider a scenario where the fourth generation neutrinos are long lived and have both a Dirac and Majorana mass term. Such neutrinos can be as light as 40 GeV and can be the dominant decay mode of the Higgs boson for Higgs masses below the W-boson threshold. We study the effect of the Majorana mass term on the Higgs branching fractions and reevaluate the Tevatron constraints on the Higgs mass. We discuss the prospects for the LHC to detect the semi-invisible Higgs decays into fourth generation neutrino pairs. Under the assumption that the lightest fourth generation neutrino is stable, it's thermal relic density can be up to 20% of the observed dark matter density in the universe. This is in agreement with current constraints on the spin dependent neutrino-neutron cross section, but can be probed by the next generation of dark matter direct detection experiments.

  14. Electromicroinjection of particles into living cells

    DOE Patents [OSTI]

    Ray, F. Andrew (Los Alamos, NM); Cram, L. Scott (Los Alamos, NM); Galey, William R. (Albuquerque, NM)

    1988-01-01

    Method and apparatus for introducing particles into living cells. Fluorescently-stained human chromosomes are introduced into cultured, mitotic Chinese hamster cells using electromicroinjection. The recipient cells frequently survived the physiological perturbation imposed by a successful chromosome injection. Successfully injected recipient cells maintained viability as evidenced by their ability to be expanded. The technique relies on the surface charge of fluorescently stained chromosomes and their ability to be attracted and repelled to and from the tip of a micropipette. The apparatus includes a micropipette having a tip suitable for piercing the membrane of a target cell and an electrode inserted into the lumen thereof. The target cells and suspended particles are located in an electrically conducted solution, and the lumen of the micropipette is filled with an electrically conducting solution which contacts the electrode located therein. A second electrode is also located in the conducting solution containing the target cells and particles. Voltages applied to the electrode within the micropipette attract the particles to the region of the tip thereof. The particles adhere to the surface of the micropipette with sufficient force that insertion of the micropipette tip and attached particle through the membrane of a target cell will not dislodge the particle. By applying a voltage having the opposite polarity of the attraction voltage, the particles are expelled from the micropipette to which is then withdrawn from the cell body.

  15. Application of Stable Isotope Probing to Identify RDX-degrading Bacteria in Groundwater 

    E-Print Network [OSTI]

    Cho, Kun-Ching

    2013-12-09

    - and fully-labeled ^(15)N-RDX to identify microorganisms capable of utilizing RDX and its metabolites as carbon and/or nitrogen sources in groundwater microcosms, and to associate active RDX-degrading microbial communities in responding to engineered...

  16. Dynamic estimation of specific growth rates and concentrations of bacteria for the anaerobic digestion

    E-Print Network [OSTI]

    Boyer, Edmond

    measured quantities ­ the dilution rate and the flow rates of methane and carbon dioxide in the biogas by microorganisms into biogas (methane and carbon dioxide) and digestate (natural manure) in the absence of oxygen [1, 2, 6]. The biogas is an additional energy source and the methane is a greenhouse gas

  17. Potential Role of Nitrite for Abiotic Fe(II) Oxidation and Cell Encrustation during Nitrate Reduction by Denitrifying Bacteria

    E-Print Network [OSTI]

    Konhauser, Kurt

    Reduction by Denitrifying Bacteria Nicole Klueglein,a Fabian Zeitvogel,b York-Dieter Stierhof,c Matthias and microoxic conditions. While most of the mix- otrophic nitrate-reducing Fe(II)-oxidizing bacteria become assemblage of Fe(II)-oxidizing bacteria in nature and compli- cates our ability to delineate microbial Fe

  18. Bacteria will help baby make 80 Sarah Staples. The Ottawa Citizen Ottawa, Ont.:Aug 17, 2004. p. A3

    E-Print Network [OSTI]

    Seroude, Laurent

    Bacteria will help baby make 80 Sarah Staples. The Ottawa Citizen Ottawa, Ont.:Aug 17, 2004. p. A3 Abstract (Article Summary) Exposing the insect dro-sophila to bacteria at various moments of its notoriously short existence, the researchers found if bacteria were successful in latching onto a host fly

  19. Layer-by-layer carbon nanotube bio-templates for in situ monitoring of the metabolic activity of nitrifying bacteria

    E-Print Network [OSTI]

    Lynch, Jerome P.

    of nitrifying bacteria Kenneth J. Loh*a , Jeremy S. Guestb , Genevieve Hob , Jerome P. Lynchb , Nancy G. Loveb of nitrifying bacteria in wastewater effluent environments (i.e., to monitor the nitrification process). Using1 . In short, nitrification utilizes autotrophic bacteria to oxidize ammonia present in wastewater

  20. An extension of generalized Taylor dispersion in unbounded homogeneous shear flows to run-and-tumble chemotactic bacteria

    E-Print Network [OSTI]

    Bearon, Rachel

    -and-tumble chemotactic bacteria R. N. Bearon School of Oceanography, Box 357940, University of Washington, Seattle of flow, the biased random walk of bacteria such as Escherichia coli is modeled by straight runs. In the well-studied situation of weak bias in tumble rate, bacteria disperse over a diffusive time scale

  1. JOURNAL OF OPTIMIZATION THEORY AND APPLICATIONS: Vol. 115, No. 3, pp. 603628, December 2002 ( 2002) Biomimicry of Social Foraging Bacteria

    E-Print Network [OSTI]

    ) Biomimicry of Social Foraging Bacteria for Distributed Optimization: Models, Principles, and Emergent the social foraging behavior of E. coli and M. xanthus bacteria and develop simulation models based that the models of both species of bacteria exhibit the property identified by Grunbaum that postulates

  2. Available online at www.sciencedirect.com Exposing plasmids as the Achilles' heel of drug-resistant bacteria

    E-Print Network [OSTI]

    Hergenrother, Paul J.

    -resistant bacteria Julia J Williams1 and Paul J Hergenrother2 Many multidrug-resistant bacterial pathogens harbor of these plasmids often enables bacteria to survive in the presence of antibiotics, it is possible that plasmids highlights three recently described strategies designed to specifically combat bacteria harboring

  3. Bacteria with tiny projections can make efficient fuel cells September 7th, 2009 -3:52 pm ICT by ANI -

    E-Print Network [OSTI]

    Lovley, Derek

    Bacteria with tiny projections can make efficient fuel cells September 7th, 2009 - 3:52 pm ICT in fuel cells than bacteria with a smooth surface. The team was led by Professor Derek Lovley from they called KN400 that grew prolifically on the graphite anodes of fuel cells. The bacteria formed a thick

  4. Estimating Bacteria Emissions from Inversion of Atmospheric Transport: Sensitivity to Modelled Particle Characteristics

    SciTech Connect (OSTI)

    Burrows, Susannah M.; Rayner, Perter; Butler, T.; Lawrence, M.

    2013-06-04

    Model-simulated transport of atmospheric trace components can be combined with observed concentrations to obtain estimates of ground-based sources using various inversion techniques. These approaches have been applied in the past primarily to obtain source estimates for long-lived trace gases such as CO2. We consider the application of similar techniques to source estimation for atmospheric aerosols, by using as a case study the estimation of bacteria emissions from different ecosystem regions in the global atmospheric chemistry and climate model ECHAM5/MESSy-Atmospheric Chemistry (EMAC). Simulated particle concentrations in the tropopause region and at high latitudes, as well as transport of particles to tundra and land ice regions are shown to be highly sensitive to scavenging in mixed-phase clouds, which is poorly characterized in most global climate models. This may be a critical uncertainty in correctly simulating the transport of aerosol particles to the Arctic. Source estimation via Monte Carlo Markov Chain is applied to a suite of sensitivity simulations and the global mean emissions are estimated. We present an analysis of the partitioning of uncertainties in the global mean emissions that are attributable to particle size, CCN activity, the ice nucleation scavenging ratios for mixed-phase and cold clouds, and measurement error. Uncertainty due to CCN activity or to a 1 um error in particle size is typically between 10% and 40% of the uncertainty due to data uncertainty, as measured by the 5%-ile to 95%-ile range of the Monte Carlo ensemble. Uncertainty attributable to the ice nucleation scavenging ratio in mized-phase clouds is as high as 10% to 20% of the data uncertainty. Taken together, the four model 20 parameters examined contribute about half as much to the uncertainty in the estimated emissions as do the measurements. This was a surprisingly large contribution from model uncertainty in light of the substantial data uncertainty, which ranges from 81% to 870% for each of ten ecosystems for this case study. The effects of these and other model parameters in contributing to the uncertainties in the transport of atmospheric aerosol particles should be treated explicitly and systematically in both forward and inverse modelling studies.

  5. The battle of bacteria: Agencies, stakeholders focusing on restoring water quality 

    E-Print Network [OSTI]

    Foust, Margaret

    2010-01-01

    stream_source_info The battle of bacteria.pdf.txt stream_content_type text/plain stream_size 5899 Content-Encoding ISO-8859-1 stream_name The battle of bacteria.pdf.txt Content-Type text/plain; charset=ISO-8859-1 txH2O... | pg. 20 Story by Margaret Foust Bacteria is the No. 1 pollutant of water in Texas, causing many of the state?s water bodies to be placed on the Texas Water Quality Inventory and 303(d) List for failing to meet contact recreation use standards...

  6. The battle of bacteria: Agencies, stakeholders focusing on restoring water quality 

    E-Print Network [OSTI]

    Foust, Margaret

    2011-01-01

    stream_source_info The battle of bacteria.pdf.txt stream_content_type text/plain stream_size 5899 Content-Encoding ISO-8859-1 stream_name The battle of bacteria.pdf.txt Content-Type text/plain; charset=ISO-8859-1 txH2O... | pg. 20 Story by Margaret Foust Bacteria is the No. 1 pollutant of water in Texas, causing many of the state?s water bodies to be placed on the Texas Water Quality Inventory and 303(d) List for failing to meet contact recreation use standards...

  7. Shybot: Friend-Stranger Interaction for Children Living with Autism

    E-Print Network [OSTI]

    Shybot: Friend-Stranger Interaction for Children Living with Autism Abstract This paper presents this simple social interaction to open up a new direction for intervention for children living with autism. We hope that from minimal social interaction, a child with autism or social anxiety disorders could

  8. Percolation Approach to Study Connectivity in Living Neural Networks

    E-Print Network [OSTI]

    Moses, Elisha

    Percolation Approach to Study Connectivity in Living Neural Networks Jordi Soriano, Ilan Breskin distribution and not a power law one. Keywords: neural networks, graphs, connectivity, percolation, giant as the fundamental feature to understand the potential of a living neural network. Unravelling the detailed

  9. Root Modeling: Estimating Storage, Live, and Dead Pool

    E-Print Network [OSTI]

    Post, Wilfred M.

    Root Modeling: Estimating Storage, Live, and Dead Pool Turnover Times; Storage Inputs to New Root to choose best-fit parameters · "Storage" simulations with in-growth cores · Live and dead pool simulations Atmosphere East Atmosphere; 1 SD East tree rings f(Average respiration and soil gas) Modeled GSD = 1.3 Range

  10. Carbon nanotubes as photoacoustic molecular imaging agents in living mice

    E-Print Network [OSTI]

    Khuri-Yakub, Butrus T. "Pierre"

    Carbon nanotubes as photoacoustic molecular imaging agents in living mice ADAM DE LA ZERDA1 not shown to target a diseased site in living subjects. Here we show that single-walled carbon nanotubes were verified ex vivo using Raman microscopy. Photoacoustic imaging of targeted single-walled carbon

  11. Long Lived Charged Massive Particles and Big Bang Nucleosynthesis

    E-Print Network [OSTI]

    Kazunori Kohri; Fumihiro Takayama

    2006-11-06

    We consider Big Bang Nucleosynthesis(BBN) with long lived charged massive particles. Before decaying, the long lived massive particles recombines with a light element to form a bound state like a hydrogen atom. We discuss the possible change of primordial light element abundances due to formations of such bound states.

  12. Rocky Flats Plant Live-Fire Range Risk Analysis Report

    SciTech Connect (OSTI)

    Nicolosi, S.L.; Rodriguez, M.A.

    1994-04-01

    The objective of the Live-Fire Range Risk Analysis Report (RAR) is to provide an authorization basis for operation as required by DOE 5480.16. The existing Live-Fire Range does not have a safety analysis-related authorization basis. EG&G Rocky Flats, Inc. has worked with DOE and its representatives to develop a format and content description for development of an RAR for the Live-Fire Range. Development of the RAR is closely aligned with development of the design for a baffle system to control risks from errant projectiles. DOE 5480.16 requires either an RAR or a safety analysis report (SAR) for live-fire ranges. An RAR rather than a SAR was selected in order to gain flexibility to more closely address the safety analysis and conduct of operation needs for a live-fire range in a cost-effective manner.

  13. Final technical report for project titled Quantitative Characterization of Cell Aggregation/Adhesion as Predictor for Distribution and Transport of Microorganisms in Subsurface Environment

    SciTech Connect (OSTI)

    Gu, April Z; Wan, Kai-tak

    2014-09-02

    This project aims to explore and develop enabling methodology and techniques for nano-scale characterization of microbe cell surface contact mechanics, interactions and adhesion quantities that allow for identification and quantification of indicative properties related to microorganism migration and transport behavior in porous media and in subsurface environments. Microbe transport has wide impact and therefore is of great interest in various environmental applications such as in situ or enhanced subsurface bioremediation,filtration processes for water and wastewater treatments and protection of drinking water supplies. Although great progress has been made towards understanding the identities and activities of these microorganisms in the subsurface, to date, little is known of the mechanisms that govern the mobility and transport of microorganisms in DOE’s contaminated sites, making the outcomes of in situ natural attenuation or contaminant stability enhancement unpredictable. Conventionally, movement of microorganisms was believed to follows the rules governing solute (particle) transport. However, recent studies revealed that cell surface properties, especially those pertaining to cell attachment/adhesion and aggregation behavior, can cause the microbe behavior to deviate from non-viable particles and hence greatly influence the mobility and distribution of microorganisms in porous media.This complexity highlights the need to obtain detailed information of cell-cell and cell-surface interactions in order to improve and refine the conceptual and quantitative model development for fate and transport of microorganisms and contaminant in subsurface. Traditional cell surface characterization methods are not sufficient to fully predict the deposition rates and transport behaviors of microorganism observed. A breakthrough of methodology that would allow for quantitative and molecular-level description of intrinsic cell surface properties indicative for cell-surface interactions is essential for the field. To tackle this, we have developed a number of new Bio-nanomechanical techniques, including reflection interference contrast microscopy (RICM) and bio-AFM (Atomic Force Microscopy), for cell adhesion-detachment measurement of the long-range surface interactions, in combination with mathematical modeling, which would allow us to characterize the mechanical behavior from single cell to multi-cell aggregate, critical thresholds for large scale coaggregation and transportation of cells and aggregates in the presence of long range inter-surface forces etc. Although some technical and mathematical challenges remain, the preliminary results promise great breakthrough potential. In this study, we investigated the cellular surface characteristics of representative bio-remediating microorganisms relevant to DOE IFRC (Integrated Field-Scale Subsurface Research Challenges) sites and their transport behaviors in porous media, aiming to draw a groundbreaking correlation between the micro-scale genetic and biological origin-based cell surface properties, the consequent mechanical adhesion and aggregation behaviors, and the macro-scale microbial mobility and retention in porous media, which are unavailable in the literature. The long-term goal is to significantly improve the mechanistic and quantitative understanding of microbial mobility, sorption, and transport within reactive transport models as needed to manipulate subsurface contaminant fate and transport predictions.

  14. Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

    DOE Patents [OSTI]

    Bavykin, Sergei G. (Darien, IL); Mirzabekov, Andrei D. (Moscow, RU)

    2007-10-30

    The present invention is directed to a novel method of discriminating a highly infectious bacterium Bacillus anthracis from a group of closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations. The identification and analysis of these sequence variations enables positive discrimination of isolates of the B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed probes, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

  15. Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

    DOE Patents [OSTI]

    Bavykin, Sergei G. (Darien, IL); Mirzabekova, legal representative, Natalia V. (Westmont, IL); Mirzabekov, deceased, Andrei D. (Westmont, IL)

    2007-12-04

    The present invention relates to methods and compositions for using nucleotide sequence variations of 16S and 23S rRNA within the B. cereus group to discriminate a highly infectious bacterium B. anthracis from closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations and discriminate B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed samples, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

  16. Growth-rate dependent partitioning of RNA polymerases in bacteria

    E-Print Network [OSTI]

    Stefan Klumpp; Terence Hwa

    2008-12-11

    Physiological changes which result in changes in bacterial gene expression are often accompanied by changes in the growth rate for fast adapting enteric bacteria. Since the availability of RNA polymerase (RNAP) in cells is dependent on the growth rate, transcriptional control involves not only the regulation of promoters, but also depends on the available (or free) RNAP concentration which is difficult to quantify directly. Here we develop a simple physical model describing the partitioning of cellular RNAP into different classes: RNAPs transcribing mRNA and ribosomal RNA (rRNA), RNAPs non-specifically bound to DNA, free RNAP, and immature RNAP. Available experimental data for E. coli allow us to determine the two unknown parameters of the model and hence deduce the free RNAP concentration at different growth rates. The results allow us to predict the growth-rate dependence of the activities of constitutive (unregulated) promoters, and to disentangle the growth-rate dependent regulation of promoters (e.g., the promoters of rRNA operons) from changes in transcription due to changes in the free RNAP concentration at different growth rates. Our model can quantitatively account for the observed changes in gene expression patterns in mutant E. coli strains with altered levels of RNAP expression without invoking additional parameters. Applying our model to the case of the stringent response following amino acid starvation, we can evaluate the plausibility of various scenarios of passive transcriptional control proposed to account for the observed changes in the expression of rRNA and biosynthetic operons.

  17. Biomedical Microdevices 3:3, 201209, 2001 # 2001 Kluwer Academic Publishers. Manufactured in The Netherlands.

    E-Print Network [OSTI]

    Bashir, Rashid

    of the live microorganism Listeria innocua injected into the chip demonstrate an easy method for detecting of small quantities of pathogenic bacteria or toxigenic substances in food, bodily ¯uids, tissue samples, soil, etc. In applications such as the screening of food products for the presence of pathogenic

  18. Genetically modified microorganisms for

    E-Print Network [OSTI]

    Angenent, Lars T.

    of our understanding of microbial reactions in microbial fuel cells (MFCs), or more generally to tailor these microbial reactions to optimize the desired functionality. We are still in the infancy

  19. Radiation-resistant microorganism

    DOE Patents [OSTI]

    Fliermans, Carl B.

    2010-06-15

    An isolated and purified bacterium is provided which was isolated from a high-level radioactive waste site of mixed waste. The isolate has the ability to degrade a wide variety of organic contaminants while demonstrating high tolerance to ionizing radiation. The organism is uniquely suited to bioremediation of a variety or organic contaminants while in the presence of ionizing radiation.

  20. Reduction of Antibiotic-Resistant Bacteria Present in Food Animal Manures by Composting and Anaerobic Digestion

    E-Print Network [OSTI]

    Jones, Michelle

    Reduction of Antibiotic-Resistant Bacteria Present in Food Animal Manures by Composting digestion and composting at mesophilic or moderate temperature significantly reduced the antimicrobial resistance in animal manure. The most effective treatment was composting at thermophilic or high temperature

  1. The YopJ superfamily in plant-associated bacteria JENNIFER D. LEWIS1,2

    E-Print Network [OSTI]

    Review The YopJ superfamily in plant-associated bacteria JENNIFER D. LEWIS1,2 , AMY LEE1,2 , WENBO). ETI relies on resistance (R) proteins, which characteristically have either a coiled-coil (CC) or toll

  2. Reassessment of the Lineage Fusion Hypothesis for the Origin of Double Membrane Bacteria

    E-Print Network [OSTI]

    Swithers, Kristen S.

    In 2009, James Lake introduced a new hypothesis in which reticulate phylogeny reconstruction is used to elucidate the origin of Gram-negative bacteria (Nature 460: 967–971). The presented data supported the Gram-negative ...

  3. Geek-Up[09.24.10] -- Magical BEANs, Combating Bacteria's Resistance...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    are comprised of crystal structures in the membrane, remove heavy-metal toxins from bacteria cells. Team lead Edward Yu of Iowa State explains, "We want to understand the...

  4. Caenorhabditis elegans NPR-1–mediated behaviors are suppressed in the presence of mucoid bacteria

    E-Print Network [OSTI]

    Reddy, Kirthi C.

    Caenorhabditis elegans exhibits a diverse range of behaviors in response to bacteria. The presence of bacterial food influences C. elegans aerotaxis, aggregation, locomotion, and pathogen avoidance behaviors through the ...

  5. Amoebae/bacteria consortia and uses for degrading wastes and contaminants

    DOE Patents [OSTI]

    Tyndall, Richard L. (Clinton, TN)

    1996-01-01

    A method of altering trinitrotoluene includes the steps of: providing an amoeba/bacteria consortium, particularly ATCC 40908 or a mutant thereof possessing all the identifying characteristics thereof; and contacting the consortium with trinitrotoluene to alter the trinitrotoluene.

  6. Nano scale devices for plasmonic nanolithography and rapid sensing of bacteria 

    E-Print Network [OSTI]

    Seo, Sungkyu

    2009-05-15

    This dissertation contains two different research topics. One is a ‘Nano Scale Device for Plasmonic Nanolithography – Optical Antenna’ and the other is a ‘Nano Scale Device for Rapid Sensing of Bacteria – SEPTIC’. Since these two different research...

  7. Development of Methodology and Characterization of Ruminal Lipase-Producing Bacteria In Vitro 

    E-Print Network [OSTI]

    Edwards, Holly Danielle

    2012-07-16

    .................................................................................................... xi CHAPTER I INTRODUCTION ................................................................................ 1 II LITERATURE REVIEW ..................................................................... 3 Digestion and Health Effects... Effects of Amide Protected Lipids and Lipid Encapsulation ...... 24 III DEVELOPMENT OF NON FORAGE BASED INCUBATION SYSTEMS FOR CULTURING ANAEROBIC RUMINAL LIPASE- PRODUCING BACTERIA IN VITRO...

  8. Population Genomics of Early Events in the Ecological Differentiation of Bacteria

    E-Print Network [OSTI]

    Shapiro, B. J.

    Genetic exchange is common among bacteria, but its effect on population diversity during ecological differentiation remains controversial. A fundamental question is whether advantageous mutations lead to selection of clonal ...

  9. Bacteria can help convert waste to power IANS 7 September 2009, 02:59pm IST

    E-Print Network [OSTI]

    Lovley, Derek

    they called KN400 that grew prolifically on the graphite anodes of fuel cells. The bacteria formed a thick bio batteries if they fail but to be successful they need to have an efficient and long lasting source of power

  10. Enhanced killing of antibiotic-resistant bacteria enabled by massively parallel combinatorial genetics

    E-Print Network [OSTI]

    Ding, Huiming

    New therapeutic strategies are needed to treat infections caused by drug-resistant bacteria, which constitute a major growing threat to human health. Here, we use a high-throughput technology to identify combinatorial ...

  11. The incidence and significance of anaerobic bacteria in the equine uterus 

    E-Print Network [OSTI]

    Bolinger, Dean Roger

    1987-01-01

    THE INCIDENCE AND SIGNIFICANCE OF ANAEROBIC BACTERIA IN THE EQUINE UTERUS A Thesis by DEAN ROGER BOLINGER Submitted to the Graduate College of Texas A&M University in partial fulfillment of the requirements of the degree of MASTER OF SCIENCF.... December I 987 Major Subject: Veterinary Medical Sciences THE INCIDENCE AND SIGNIFICANCE OF ANAEROBIC BACTERIA IN THE EQUINE UTERUS A Thesis by DEAN ROGER BOLINGER Approved as to style and content by: Ronnie G. Elmore (Chairman of Committee...

  12. Generation of Long-Lived Isomeric States via Bremsstrahlung Irradiation

    E-Print Network [OSTI]

    Yao Cheng; Bing Xia; Chuanxiang Tang; Yinong Liu; Qingxiu Jin

    2006-11-08

    A method to generate long-lived isomeric states effectively for Mossbauer applications is reported. We demonstrate that this method is better and easier to provide highly sensitive Mossbauer effect of long-lived isomers (>1ms) such as 103Rh. Excitation of (gamma,gamma) process by synchrotron radiation is painful due mainly to their limited linewidth. Instead,(gamma,gamma') process of bremsstrahlung excitation is applied to create these long-lived isomers. Isomers of 45Sc, 107Ag, 109Ag, and 103Rh have been generated from this method. Among them, 103Rh is the only one that we have obtained the gravitational effect at room temperature.

  13. L\\'evy Fluctuations and Tracer Diffusion in Dilute Suspensions of Algae and Bacteria

    E-Print Network [OSTI]

    Zaid, Irwin M; Yeomans, Julia M

    2010-01-01

    Swimming microorganisms rely on effective mixing strategies to achieve efficient nutrient influx. Recent experiments, probing the mixing capability of unicellular biflagellates, revealed that passive tracer particles exhibit anomalous non-Gaussian diffusion when immersed in a dilute suspension of self-motile Chlamydomonas reinhardtii algae. Qualitatively, this observation can be explained by the fact that the algae induce a fluid flow that may occasionally accelerate the colloidal tracers to relatively large velocities. A satisfactory quantitative theory of enhanced mixing in dilute active suspensions, however, is lacking at present. In particular, it is unclear how non-Gaussian signatures in the tracers' position distribution are linked to the self-propulsion mechanism of a microorganism. Here, we develop a systematic theoretical description of anomalous tracer diffusion in active suspensions, based on a simplified tracer-swimmer interaction model that captures the typical distance scaling of a microswimmer'...

  14. Continuum- based computational models of biological living cell 

    E-Print Network [OSTI]

    Cheng, Feifei

    2009-05-15

    All living creatures, despite their profound diversity, share a common architectural building block: the cell. Cells are the basic functional units of life, yet are themselves comprised of numerous components with distinct ...

  15. The living commons : a spatial theory for biological design

    E-Print Network [OSTI]

    Telhan, Orkan

    2013-01-01

    Biological design is as ancient as human civilization. For thousands of years, living systems and natural processes have been manipulated by humans and their biological outcomes have been customized for different purposes. ...

  16. Ask a scientist: Nanotech in our lives | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ask a scientist: Nanotech in our lives June 1, 2014 Tweet EmailPrint Is there nanotechnology already in my consumer products? Carrado Gregar: I just saw a report that named...

  17. Optimization of environmental control to fit living space requirements 

    E-Print Network [OSTI]

    Eckmann, Maxim S

    2013-02-22

    This study examines the application of Environmental Control Systems (ECSs) for people with disabilities who live in the dormitory. ECSs allow people with disabilities to control appliances in their homes and parameters ...

  18. Spontaneous fission half-lives for ground state nuclides

    SciTech Connect (OSTI)

    Holden, N.E. (Brookhaven National Lab., Upton, NY (United States)); Hoffman, D.C. (Lawrence Berkeley Lab., CA (United States))

    1991-01-01

    Measurements of the spontaneous fission half-lives of nuclides of elements Z = 90 to 107 have been compiled and evaluated. Recommended values are presented. 126 refs., 96 tabs.

  19. University Housing Designing Sustainable Communities for Living and Learning

    E-Print Network [OSTI]

    Almor, Amit

    The Mission Learning Center for Sustainable Futures Green Learning Community Student Engagement Green Living Public Events Green Education University and Community Outreach Community Building Our Home:Physical Features LEED Certification Sustainable Sites Materials and Resources Energy and Atmosphere Indoor

  20. Live Webcast on Recent Wind Energy Technology Advances

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webcast titled “Recent Wind Technology Advances” on April 16, 2014, from 3:00 to 4:00 p.m. Eastern Standard Time.

  1. Acting Biomass Program Manager Dr. Valerie Reed to Host Live...

    Broader source: Energy.gov (indexed) [DOE]

    December 16th, the Energy Department (@energy) will be hosting a live Twitter Q&A on biofuels with Dr. Valerie Reed, Acting Manager of the Biomass Program. Dr. Reed holds a Ph. D....

  2. DYNAMIC MODELLING OF LIVING ANIONIC SOLUTION POLYMERIZATION OF STYRENE/BUTADIENE/DIVINYLBENZENE

    E-Print Network [OSTI]

    Schittkowski, Klaus

    DYNAMIC MODELLING OF LIVING ANIONIC SOLUTION POLYMERIZATION OF STYRENE/BUTADIENE model for the living anionic solution polymerization of styrene/butadiene/divinylbenzene in a continuous kinetic reactor model for the living anionic solution polymerization of styrene/butadiene

  3. Turning Bacteria into Biofuel: Development of an Integrated Microbial Electrocatalytic (MEC) System for Liquid Biofuel Production from CO2

    SciTech Connect (OSTI)

    2010-08-01

    Electrofuels Project: LBNL is improving the natural ability of a common soil bacteria called Ralstonia eutropha to use hydrogen and carbon dioxide for biofuel production. First, LBNL is genetically modifying the bacteria to produce biofuel at higher concentrations. Then, LBNL is using renewable electricity obtained from solar, wind, or wave power to produce high amounts of hydrogen in the presence of the bacteria—increasing the organism’s access to its energy source and improving the efficiency of the biofuel-creation process. Finally, LBNL is tethering electrocatalysts to the bacteria’s surface which will further accelerate the rate at which the organism creates biofuel. LBNL is also developing a chemical method to transform the biofuel that the bacteria produce into ready-to-use jet fuel.

  4. The Marine Live Bait Trade in California: A Pathway for Introduction of Non-Indigenous Species?

    E-Print Network [OSTI]

    Passarelli, Bruno

    2010-01-01

    To investigate the transport of NIS into California via theCalifornia via the Marine Live Bait Trade TransportCalifornia via the Marine Live Bait Trade Transport

  5. The Administration of Intranasal Live Attenuated Influenza Vaccine Induces Changes in the Nasal Microbiota and Nasal Epithelium Gene Expression Profile

    E-Print Network [OSTI]

    Tarabichi, Yasir

    2015-01-01

    56 vi The Administration of Intranasal Live AttenuatedCALIFORNIA Los Angeles The Administration of Intranasal LiveABSTRACT OF THE THESIS The Administration of Intranasal Live

  6. Liquid Fuel From Renewable Electricity and Bacteria: Electro-Autotrophic Synthesis of Higher Alcohols

    SciTech Connect (OSTI)

    None

    2010-07-01

    Electrofuels Project: UCLA is utilizing renewable electricity to power direct liquid fuel production in genetically engineered Ralstonia eutropha bacteria. UCLA is using renewable electricity to convert carbon dioxide into formic acid, a liquid soluble compound that delivers both carbon and energy to the bacteria. The bacteria are genetically engineered to convert the formic acid into liquid fuel—in this case alcohols such as butanol. The electricity required for the process can be generated from sunlight, wind, or other renewable energy sources. In fact, UCLA’s electricity-to-fuel system could be a more efficient way to utilize these renewable energy sources considering the energy density of liquid fuel is much higher than the energy density of other renewable energy storage options, such as batteries.

  7. Inoculation of beef with lactic-acid bacteria prior to vacuum packaging 

    E-Print Network [OSTI]

    Hall, Lester Cedric

    1978-01-01

    August 1978 Major Subject: Animal Science INOCULATION OF BEEF WITH LACTIC-ACID BACTERIA PRIOR TO VACUUM PACKAGING A Thesis by LESTER CEDRIC HALL Approved as to style and content by: 1 ( (Co- hairman of C mmittee) (Co-Chakrm 'g of Committee) (He... d of Department ) (Member) August 1978 ABSTRACT Inoculation of Beef with Lactic-Acid Bacteria Prior to Vacuum Packaging. (August 1978) Lester Cedric Hall, B. S. , Texas A&M University Co-Chairmen of Advisory Committee: Dr. G. C. Smith and Dr Z...

  8. Genetically Modified Bacteria for Fuel Production: Development of Rhodobacteria as a Versatile Platform for Fuels Production

    SciTech Connect (OSTI)

    None

    2010-07-01

    Electrofuels Project: Penn State is genetically engineering bacteria called Rhodobacter to use electricity or electrically generated hydrogen to convert carbon dioxide into liquid fuels. Penn State is taking genes from oil-producing algae called Botryococcus braunii and putting them into Rhodobacter to produce hydrocarbon molecules, which closely resemble gasoline. Penn State is developing engineered tanks to support microbial fuel production and determining the most economical way to feed the electricity or hydrogen to the bacteria, including using renewable sources of power like solar energy.

  9. Investigation of anaerobic bacteria associated with mortality in grey mullet (Mugil cephalus) and redfish (Sciaenops ocellatus) 

    E-Print Network [OSTI]

    Henley, Michael Waite

    1976-01-01

    INVEST1GATION OF ANAEROBIC BACTERIA ASSOCIATED WITH NDRTALITY IN GREY MULLET t~N' I ~*h I ) AND REDYIRH (~H' o ll t A Thesis MICHAEL WAITE HENLEY f Submitted to the Graduate College of Texas ARM University in partial fulfillment... of the requirement for the degree of MASTER OF SCIENCE August 1976 Major Subject: Veterinary Microbiology INVESTIGATION OF ANAEROBIC BACTERIA ASSOCIATED NITH liIIH NDR IlDIIY IN GREY NUDE EI 1M~I ~ll END REDPI H l~R' ll t 1 A Thesis !G!ICHAEL WAITE HENLEY...

  10. Factors affecting the recovery of bacteria in freeze-dried model systems 

    E-Print Network [OSTI]

    Custer, Carl Steven

    1970-01-01

    FACTORS AFFECTING THE RECOVERY OF BACTERIA IN FREEZE-DRIED NODEL SYSTENS A Thesis by CARL STEVEN CUSTER Submitted to the Graduate College of Texas A&N University in partial fulfillment of the requirement for tbe degree of MASTER OF SCIENCE... December 1970' Najor Subject: Food Technology FACTORS AFFECTING THE RECOVERY OF BACTERIA IN FREEZE-DRIED MODEL SYSTEMS A Thesis CARL STEVEN CUSTER Approved as to sty1e and content by: Chairman of Com tee) Head of Departme (Member) (Member) (Member...

  11. Saving fourth generation and baryon number by living long

    E-Print Network [OSTI]

    Hitoshi Murayama; Vikram Rentala; Jing Shu; Tsutomu T. Yanagida

    2010-12-01

    Recent studies of precision electroweak observables have led to the conclusion that a fourth generation is highly constrained. However, we point out that a long-lived fourth generation can reopen a large portion of the parameter space. In addition, it preserves baryon and lepton asymmetries against sphaleron erasure even if $B-L=0$. It opens up the possibility of exact $B-L$ symmetry and hence Dirac neutrinos. The fourth generation can be observed at the LHC with unique signatures of long-lived particles in the near future.

  12. Living well, doing good | Y-12 National Security Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformationJesse BergkampCenter (LMI-EFRC) ProximityCenterLeeincreases |Living aLiving

  13. The Sustainability FYE Cluster The Sustainability FYE Cluster will make sustainable urban living a core experience for First

    E-Print Network [OSTI]

    The Sustainability FYE Cluster The Sustainability FYE Cluster will make sustainable urban living University. The Sustainability community infuses the learning-living community with practical and theoretical approaches to sustainable living, merging students' living community with unique academic and field

  14. Genomics Encyclopedia of Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB): a resource for microsymbiont genomes (2013 DOE JGI Genomics of Energy and Environment 8th Annual User Meeting)

    SciTech Connect (OSTI)

    Reeve, Wayne [Murdoch University

    2013-03-01

    Wayne Reeve of Murdoch University on "Genomics Encyclopedia of Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB): a resource for microsymbiont genomes" at the 8th Annual Genomics of Energy & Environment Meeting on March 27, 2013 in Walnut Creek, Calif.

  15. Aquat. Living Resour. 23, 267276 (2010) c EDP Sciences, IFREMER, IRD 2010

    E-Print Network [OSTI]

    Pierce, Graham

    2010-01-01

    www.alr-journal.org Aquatic Living Resources Mesoscale effects of aquaculture installations on benthic

  16. Discordance between living and death assemblages as evidence for anthropogenic ecological change

    E-Print Network [OSTI]

    . ecological baseline eutrophication marine communities paleoecology Human activities affect living systems

  17. Final Report on MAPPR Project: The Detroit Living Wage Ordinance: Will it Reduce Urban Poverty?

    E-Print Network [OSTI]

    Riley, Shawn J.

    Final Report on MAPPR Project: The Detroit Living Wage Ordinance: Will it Reduce Urban Poverty? David Neumark May 30, 2001 #12;Detroit's Living Wage Ordinance The Detroit Living Wage Ordinance passed in the fall of 1998. As in other major urban areas passing living wage ordinances in the 1990s, Detroit

  18. Relative Susceptibility and Transcriptional Response of Nitrogen Cycling Bacteria to Quantum Dots

    E-Print Network [OSTI]

    Alvarez, Pedro J.

    -100 nm) consisting of a metalloid crystalline core, such as CdSe or CdTe, and an encapsulating ZnS or Cd and photostability, which are useful for biomedical imaging, therapeutic applications, and solar cells.4-11 QDs can be toxic to bacteria,12,13 invertebrates,14 and to animal15 and human cells,16 and can damage

  19. in-house windrow composting Many of the water bodies that are impaired by bacteria

    E-Print Network [OSTI]

    in-house windrow composting Many of the water bodies that are impaired by bacteria throughout from new and existing facilities. One such BMP is in-house windrow composting (IWC) of poultry litter. The Environmental Effects of In-House Windrow Composting of Poultry Litter project is demonstrating

  20. ABSTRACTABSTRACT Microbial fuel cells (MFCs) are devices that utilize bacteria to

    E-Print Network [OSTI]

    ) applications are becoming a popular means of power generation within our society and Microbial fuel cells (MFCsABSTRACTABSTRACT Microbial fuel cells (MFCs) are devices that utilize bacteria to generate efficiencies in comparison to varying fuel cell components and structures. Seven single chamber MFCs were

  1. Structure-based Inhibitor Discovery against Adenylyl Cyclase Toxins from Pathogenic Bacteria That Cause Anthrax and

    E-Print Network [OSTI]

    Mrksich, Milan

    That Cause Anthrax and Whooping Cough* Received for publication, February 4, 2003, and in revised form, March bacteria that cause anthrax and whooping cough, respectively. Using the structure of the catalytic site pathogenesis and to fight against anthrax and whooping cough. The 2001 anthrax attacks in the United States

  2. Characterization of arsenic-resistant endophytic bacteria from hyperaccumulators Pteris vittata and Pteris multifida

    E-Print Network [OSTI]

    Ma, Lena

    Characterization of arsenic-resistant endophytic bacteria from hyperaccumulators Pteris vittata i g h l i g h t s 74 As-resistant endophytes from two As-hyperaccumulators were identified AsV reduction and AsIII oxidation. Endophytes from Pteris multifida were more resistant to arsenite

  3. Association with pathogenic bacteria affects life-history traits and population growth in Caenorhabditis elegans.

    E-Print Network [OSTI]

    Diaz, S. Anaid; Mooring, Eric Q.; Rens, Elisabeth G.; Restif, Olivier

    2015-03-23

    with respect to its association with pathogenic bacteria. In order to start filling the gap between the two areas, we conducted a series of experiments aiming at measuring life-history traits as well as population growth of C. elegans in response to three...

  4. Methanobactin: a copper binding compound having antibiotic and antioxidant activity isolated from methanotrophic bacteria

    DOE Patents [OSTI]

    DiSpirito, Alan A. (Ames, IA); Zahn, James A. (Harbor Beach, MI); Graham, David W. (Lawrence, KS); Kim, Hyung J. (St. Paul, MN); Alterman, Michail (Lawrence, KS); Larive, Cynthia (Lawrence, KS)

    2007-04-03

    A means and method for treating bacterial infection, providing antioxidant activity, and chelating copper using a copper binding compound produced by methanotrophic bacteria is described. The compound, known as methanobactin, is the first of a new class of antibiotics having gram-positive activity. Methanobactin has been sequenced, and its structural formula determined.

  5. Protein elemental sparing and codon usage bias are correlated among bacteria

    E-Print Network [OSTI]

    Wagner, Andreas

    Protein elemental sparing and codon usage bias are correlated among bacteria JASON G. BRAGG, in terms of the quantities of carbon (C), nitrogen (N) or sulphur (S) atoms they contain. This `elemental sparing' probably reflects selection to reduce the quantities of potentially growth- limiting elements

  6. Microbial Grazers Lab Objective: Measure the rate at which bacteria are consumed by predators.

    E-Print Network [OSTI]

    Vallino, Joseph J.

    Microbial Grazers Lab Objective: Measure the rate at which bacteria are consumed by predators. Overview ·Size based food webs ·Microbial loop concepts ·Bacterial predators ·Methods to assess microbial, they were not thought to be significant consumers of carbon or energy. microPlankton mesoPlankton #12

  7. Microfluidic capture and release of bacteria in a conical nanopore array Peng Guo,ab

    E-Print Network [OSTI]

    Zare, Richard N.

    Microfluidic capture and release of bacteria in a conical nanopore array Peng Guo,ab Eric W. Hall a microfluidic device. As an example, we demonstrate that cyanobacteria can be captured, one bacterium per pore, in a conical nanoporous membrane (CNM) integrated into a microfluidic chip. This study, to our knowledge

  8. Growth and Survival of Immature Haematobia irritans (Diptera: Muscidae) is Influenced by Bacteria Isolated from Cattle

    E-Print Network [OSTI]

    Selinger, Brent

    by Bacteria Isolated from Cattle Manure and Conspecific Larvae M. A. PEROTTI, T. J. LYSYK, L. D. KALISCHUK from cattle manure and seven species were isolated from the gut of larval horn ßy Hematobia irritans (L bacterial species were evaluated by rearing larvae in sterilized cattle manure that was inoculated

  9. Cross-stress protection in bacteria: an evolutionary perspective Ilias Tagkopoulos

    E-Print Network [OSTI]

    Tagkopoulos, Ilias

    Cross-stress protection in bacteria: an evolutionary perspective Ilias Tagkopoulos Department parameters constantly fluctuate. "Stress" is the term used when these fluctuations vary severely from optimum, and the ability of microbes to sense and respond to environmental stress is critical to their survival. Due

  10. Amoebae/bacteria consortia and uses for degrading wastes and contaminants

    DOE Patents [OSTI]

    Tyndall, R.L.

    1996-05-21

    A method is disclosed of altering trinitrotoluene. The steps include the following: providing an amoeba/bacteria consortium, particularly ATCC 40908 or a mutant which possesses all the identifying characteristics thereof; and contacting the consortium with trinitrotoluene to alter the trinitrotoluene.

  11. Memory-Assisted Exciton Diffusion in the Chlorosome Light-Harvesting Antenna of Green Sulfur Bacteria

    E-Print Network [OSTI]

    Saikin, Semion

    Memory-Assisted Exciton Diffusion in the Chlorosome Light- Harvesting Antenna of Green Sulfur of bacteriochlorophylls (BChls) enclosed by a lipid monolayer.1-4 They can capture light and transfer it in a form. In green sulfur bacteria, light energy absorbed by the rolls is transferred via a baseplate9 to the Fenna

  12. In bacteria, the two defining processes of the cell cycle, chromosome replication and segre

    E-Print Network [OSTI]

    Mekalanos, John

    In bacteria, the two defining processes of the cell cycle, chromosome replication and segre gation and Hunt in 1993 (REF. 2) as "among the most mysterious" events during the bacterial cell cycle, and its segregation. In the first part of this Opinion article, we consider when entropy will drive segrega tion

  13. Growth Temperature and Genome Size in Bacteria Are Negatively Correlated, Suggesting Genomic Streamlining

    E-Print Network [OSTI]

    Wagner, Andreas

    Growth Temperature and Genome Size in Bacteria Are Negatively Correlated, Suggesting Genomic.wagner@ieu.uzh.ch; nsabath@gmail.com. Accepted: March 25, 2013 Abstract Prokaryotic genomes are small and compact. Either this feature is caused by neutral evolution or by natural selection favoring small genomes--genome streamlining

  14. Ammonia oxidation kinetics determine niche separation of nitrifying Archaea and Bacteria

    E-Print Network [OSTI]

    de la Torre, José R.

    LETTERS Ammonia oxidation kinetics determine niche separation of nitrifying Archaea and Bacteria and the widespread distribution of these organisms in marine and terrestrial environments indicated an important role or their contribution to nitrification8 . Here we report oligotrophic ammonia oxidation kinetics and cellular

  15. Digestion of milk protein and methanol-grown bacteria protein in the preruminant calf. II. Amino acid composition of

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Digestion of milk protein and methanol-grown bacteria protein in the preruminant calf. II. Amino of milk and of methanol-grown bacteria in the terminal small intestine and the hindgut of the preruminant exclusively by skim-milk powder ; 50.5 p. 100 of the protein of the bacte- ria diet was supplied by methanol

  16. Elucidate the mechanism(s) of Hg uptake in methylating bacteria and effects on Hg methylation in nature.

    E-Print Network [OSTI]

    Post, Wilfred M.

    Objective · Elucidate the mechanism(s) of Hg uptake in methylating bacteria and effects on Hg, suggesting a possible detoxification mechanism for the formation and export of methylmercury. 1 BER Overview A New Mechanism of Mercury Uptake and Methylation in Anaerobic Bacteria Contact: Jeffra Schaefer

  17. Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wetmore, Kelly M.; Price, Morgan N.; Waters, Robert J.; Lamson, Jacob S.; He, Jennifer; Hoover, Cindi A.; Blow, Matthew J.; Bristow, James; Butland, Gareth; Arkin, Adam P.; et al

    2015-05-12

    Transposon mutagenesis with next-generation sequencing (TnSeq) is a powerful approach to annotate gene function in bacteria, but existing protocols for TnSeq require laborious preparation of every sample before sequencing. Thus, the existing protocols are not amenable to the throughput necessary to identify phenotypes and functions for the majority of genes in diverse bacteria. Here, we present a method, random bar code transposon-site sequencing (RB-TnSeq), which increases the throughput of mutant fitness profiling by incorporating random DNA bar codes into Tn5 and mariner transposons and by using bar code sequencing (BarSeq) to assay mutant fitness. RB-TnSeq can be used with anymore »transposon, and TnSeq is performed once per organism instead of once per sample. Each BarSeq assay requires only a simple PCR, and 48 to 96 samples can be sequenced on one lane of an Illumina HiSeq system. We demonstrate the reproducibility and biological significance of RB-TnSeq with Escherichia coli, Phaeobacter inhibens, Pseudomonas stutzeri, Shewanella amazonensis, and Shewanella oneidensis. To demonstrate the increased throughput of RB-TnSeq, we performed 387 successful genome-wide mutant fitness assays representing 130 different bacterium-carbon source combinations and identified 5,196 genes with significant phenotypes across the five bacteria. In P. inhibens, we used our mutant fitness data to identify genes important for the utilization of diverse carbon substrates, including a putative D-mannose isomerase that is required for mannitol catabolism. RB-TnSeq will enable the cost-effective functional annotation of diverse bacteria using mutant fitness profiling. A large challenge in microbiology is the functional assessment of the millions of uncharacterized genes identified by genome sequencing. Transposon mutagenesis coupled to next-generation sequencing (TnSeq) is a powerful approach to assign phenotypes and functions to genes. However, the current strategies for TnSeq are too laborious to be applied to hundreds of experimental conditions across multiple bacteria. Here, we describe an approach, random bar code transposon-site sequencing (RB-TnSeq), which greatly simplifies the measurement of gene fitness by using bar code sequencing (BarSeq) to monitor the abundance of mutants. We performed 387 genome-wide fitness assays across five bacteria and identified phenotypes for over 5,000 genes. RB-TnSeq can be applied to diverse bacteria and is a powerful tool to annotate uncharacterized genes using phenotype data.« less

  18. October 11, 2011 Keeping In Touch . . . Impacting Lives

    E-Print Network [OSTI]

    October 11, 2011 Keeping In Touch . . . Impacting Lives SCHOOL OF ALLIED HEALTH PROFESSIONS LSUHSC · School of Allied Health Professions · 1900 Gravier Street · New Orleans, LA 70112 · 504-568-4246 · www.alliedhealth.lsuhsc.edu May 19, 2014 Growing our Health CareTeam The School of Allied Health Professions (SAHP

  19. Framing Change: Social Movement Framing in University Living Wage Movements 

    E-Print Network [OSTI]

    Metcalf, Laurie D.

    2010-01-14

    of these living wage movements, two cases, Texas A&M University and Georgetown University, were selected for this study to examine through the lens of the social movement framing perspective. Data for the cases included interviews with activists and administrators...

  20. Flow-induced gelation of living (micellar) polymers Robijn Bruinsma

    E-Print Network [OSTI]

    Ben-Shaul, Avinoam

    Flow-induced gelation of living (micellar) polymers Robijn Bruinsma Department of Physics-deformationeffectsdue to flow. Steady-statesolutions to the kinetic equationareobtained,with the correspondingmean micellar size (z) evaluatedasa function of the PecletnumberP, i.e., the dimensionlessratio of flow rate i

  1. Distribution of ranks of ?-decay half-lives

    E-Print Network [OSTI]

    Juan Miguel Campanario

    2010-11-21

    I studied the distribution of ranks of values of 2949 {\\beta}-decay half-lives according to an empirical beta law with two exponents. {\\beta}-decay half-life ranks showed good fit to a beta function with two exponents.

  2. Enhancing the Performance of High Availability Lightweight Live Migration

    E-Print Network [OSTI]

    Ravindran, Binoy

    on the primary host, and transfers the latest checkpoint to the backup host as whole-system migration. Once is that the primary host migrates the guest VM image (including CPU/memory status updates and new writes to the fileEnhancing the Performance of High Availability Lightweight Live Migration Peng Lu1, Binoy Ravindran

  3. Continental ood basalts: episodic magmatism above long-lived hotspots

    E-Print Network [OSTI]

    Johnston, Stephen T.

    November 1999 Abstract The eruption of continental flood basalt (CFB) may reflect episodic magmatism above long-lived mantle plumes. The Iceland and Yellowstone hotspots have generated successive CFB provinces in subducting oceanic lithosphere led to subsequent breakthrough and eruption of CFB. Since both mantle plume

  4. ith America focused on energy alternatives and green living,

    E-Print Network [OSTI]

    Veiga, Pedro Manuel Barbosa

    W ith America focused on energy alternatives and green living, Oklahoma State University- Tulsa. His process to produce a clean, alternative energy source could significantly reduce the use of fossil it a very attractive alternative to solar energy technology. "Thermoelectric materials are so much less

  5. Working Together to Save Lives National Weather Service Strategic Plan

    E-Print Network [OSTI]

    Working Together to Save Lives National Weather Service Strategic Plan for 2005-2010 #12;i Preface The National Oceanic and Atmospheric Administration (NOAA) Strategic Plan (http for a broader range of environmental information services. NOAA's National Weather Service (NWS) Strategic Plan

  6. Saving Lives, Time and Resources tti.tamu.edu

    E-Print Network [OSTI]

    . TTI research is widely known as an excellent value with a proven impact of saving lives, time grounds facility, environmental and emissions facility and sediment and erosion control laboratory eight offices in Texas and offices in Ann Arbor, MI; Washington, D.C.; and Mexico City. TTI also has

  7. Computational adaptive optics for live three-dimensional biological imaging

    E-Print Network [OSTI]

    Agard, David

    Computational adaptive optics for live three- dimensional biological imaging Z. Kam*, B. Hanser , M. Under their design conditions, modern microscope optics produce nearly ideal aberration-free imaging to the coverslip. When focusing into thick samples, the 3D optical characteristics of the sample itself must

  8. Shrinky Dink microbes! icrobes are living organisms smaller

    E-Print Network [OSTI]

    Lawrence, Rick L.

    Shrinky Dink microbes! M icrobes are living organisms smaller than your eyes can see in Yellowstone National Park. Scientists at Montana State University (MSU) are researching how microbes from. Some kinds of microbes, called extremophiles, thrive in places that are freezing, super hot, deep

  9. Safety and Liveness in Intelligent Intersections Hemant Kowshik1

    E-Print Network [OSTI]

    of designing intelligent intersections where traffic lights and stop signs are removed, and cars negotiate intersections are representative of complex distributed hybrid systems which need architectures and algorithms with provable safety and liveness. We propose a hybrid architecture which involves an appropriate inter- play

  10. New York City Visiting or living in New York

    E-Print Network [OSTI]

    Navigating New York City #12;·Visiting or living in New York for the first time is an interesting. #12;Learn the geography of New York City's five boroughs. 1) Manhattan 2) Brooklyn 3) Queens 4) Bronx Major airports serving New York City: 1) John F. Kennedy International Airport (JFK) 2) La

  11. Motivating Mobility: Designing for Lived Motivation in Stroke Rehabilitation

    E-Print Network [OSTI]

    Balaam, Madeline

    Motivating Mobility: Designing for Lived Motivation in Stroke Rehabilitation Madeline Balaam1 rehabilitation exercise after stroke. We report on participatory design work with four stroke survivors to develop a holistic understanding of their motivation and rehabilitation needs, and to construct and deploy

  12. Have You Visited the Living Zero Home Tour?

    Broader source: Energy.gov [DOE]

    The Living Zero Home Tour is on the road, and it may be in a city near you! If you have the chance to see the tour, you may be surprised to discover the small steps that you can take to save energy...

  13. Marshall Islands: a study of diet and living patterns

    SciTech Connect (OSTI)

    Naidu, J.R.; Greenhouse, N.A.; Knight, G.; Craighead, E.C.

    1980-07-01

    This study summarizes information on diet and living patterns for the Marshallese. The data was derived from literature, answers to questionnaires, personal observations while living with the Marshallese for periods extending from months to years, and from direct participation in their activities. The results reflect the complex interactions of many influences, such as, the gathering of local foods the receipt of food aid through programs, such as, school-lunch, typhoon-relief, food distributed to populations displaced as a result of nuclear testing, and in recent times the availability of cash for the purchase of imported foods. The results identify these influences and are therefore restricted to local food diets while recognizing that the living patterns are changing as local food gathering is replaced by other food supplies. The data will therefore provide the necessary information for input into models that will assess the radiological impacts attributable to the inhabitation of the Marshall Islands. It is recommended that this study should be continued for at least two to three years in order to more accurately identify trends in local food consumption and living patterns.

  14. Road to Discovery Blog Riding for the Lives of Children

    E-Print Network [OSTI]

    Buechler, Steven

    #12;BOSTON 6.13.11 DALLAS 7.8.11 Road to Discovery Blog Riding for the Lives of Children 2 for us. " " #12;day 1W e had the perfect day today! The weather was a bit chilly at first departed from Ara Parseghian's home in the Boston area. Ara is the grandson of Coach Ara Parseghian, one

  15. Metal-induced energy transfer for live cell nanoscopy

    E-Print Network [OSTI]

    Enderlein, Jörg

    Metal-induced energy transfer for live cell nanoscopy Alexey I. Chizhik1 *, Jan Rother2 , Ingo transfer from an optically excited donor to an acceptor. We replace the acceptor molecule with a metallic film and use the measured energy transfer efficiency from donor molecules to metal surface plasmons2

  16. Health Foods, Healthy Lives Institute Grants Awarded March 2011

    E-Print Network [OSTI]

    Amin, S. Massoud

    1 Health Foods, Healthy Lives Institute Grants Awarded March 2011 Food Safety "Prevalence of Medicine) Co-Investigators: Jeff Bender, DVM, Veterinary Public Health (College of Veterinary Medicine and Intl Medicine (School of Medicine) David Boxrud, MS, Molecular Typing Laboratory (MN Dept of Health

  17. Brazil's Right to Save Lives (NYT) 484 words

    E-Print Network [OSTI]

    Lopez-Carr, David

    Brazil's Right to Save Lives (NYT) 484 words Published: June 23, 2005 Brazil has the best anti-name drugs. Brazil can freely copy any drug commercialized before 1997, when the country began to respect are still imported and are expensive, and Brazil is spending two-thirds of its antiretroviral budget on just

  18. Living our dying: Dewey, death and aesthetic sensibility 

    E-Print Network [OSTI]

    Zgarba, Rex Jason

    1997-01-01

    the Internet on NPR's web site at www. npr. org. game called "Creatures. " In the course of the game the player receives six eggs, an incubator, and a whole virtual world in which the creatures can 'live'. They move, eat, grow, get sick, sleep and reproduce...

  19. Living in a Smart Environment Implications for the Coming Ubiquitous

    E-Print Network [OSTI]

    - ciplinary research project "Living in a Smart Environ- ment ­ Implications of Ubiquitous Computing". To show from a large-scale de- ployment of ubiquitous and pervasive computing tech- nologies. Second, it investigates issues of social compati- bility and dependability of future ubiquitous computing applications

  20. Public Health in Public Housing: Improving Health, Changing Lives

    E-Print Network [OSTI]

    Shen, Jun

    Public Health in Public Housing: Improving Health, Changing Lives National Heart, Lung, and Blood Institute Education Strategy Development Workshop S U M M A R Y R E P O R T U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES National Institutes of Health National Heart, Lung, and Blood Institute #12;#12;U

  1. Lévy Fluctuations and Tracer Diffusion in Dilute Suspensions of Algae and Bacteria

    E-Print Network [OSTI]

    Irwin M. Zaid; Jörn Dunkel; Julia M. Yeomans

    2010-09-20

    Swimming microorganisms rely on effective mixing strategies to achieve efficient nutrient influx. Recent experiments, probing the mixing capability of unicellular biflagellates, revealed that passive tracer particles exhibit anomalous non-Gaussian diffusion when immersed in a dilute suspension of self-motile Chlamydomonas reinhardtii algae. Qualitatively, this observation can be explained by the fact that the algae induce a fluid flow that may occasionally accelerate the colloidal tracers to relatively large velocities. A satisfactory quantitative theory of enhanced mixing in dilute active suspensions, however, is lacking at present. In particular, it is unclear how non-Gaussian signatures in the tracers' position distribution are linked to the self-propulsion mechanism of a microorganism. Here, we develop a systematic theoretical description of anomalous tracer diffusion in active suspensions, based on a simplified tracer-swimmer interaction model that captures the typical distance scaling of a microswimmer's flow field. We show that the experimentally observed non-Gaussian tails are generic and arise due to a combination of truncated L\\'evy statistics for the velocity field and algebraically decaying time correlations in the fluid. Our analytical considerations are illustrated through extensive simulations, implemented on graphics processing units to achieve the large sample sizes required for analyzing the tails of the tracer distributions.

  2. From Nanowires to Biofilms: An Exploration of Novel Mechanisms of Uranium Transformation Mediated by Geobacter Bacteria

    SciTech Connect (OSTI)

    REGUERA, GEMMA [Michigan State University

    2014-01-16

    One promising strategy for the in situ bioremediation of radioactive groundwater contaminants that has been identified by the SBR Program is to stimulate the activity of dissimilatory metal-reducing microorganisms to reductively precipitate uranium and other soluble toxic metals. The reduction of U(VI) and other soluble contaminants by Geobacteraceae is directly dependent on the reduction of Fe(III) oxides, their natural electron acceptor, a process that requires the expression of Geobacter’s conductive pili (pilus nanowires). Expression of conductive pili by Geobacter cells leads to biofilm development on surfaces and to the formation of suspended biogranules, which may be physiological closer to biofilms than to planktonic cells. Biofilm development is often assumed in the subsurface, particularly at the matrix-well screen interface, but evidence of biofilms in the bulk aquifer matrix is scarce. Our preliminary results suggest, however, that biofilms develop in the subsurface and contribute to uranium transformations via sorption and reductive mechanisms. In this project we elucidated the mechanism(s) for uranium immobilization mediated by Geobacter biofilms and identified molecular markers to investigate if biofilm development is happening in the contaminated subsurface. The results provided novel insights needed in order to understand the metabolic potential and physiology of microorganisms with a known role in contaminant transformation in situ, thus having a significant positive impact in the SBR Program and providing novel concept to monitor, model, and predict biological behavior during in situ treatments.

  3. ORGANIC GEOCHEMICAL STUDIES. II. THE DISTRIBUTION OF ALIPHATIC HYDROCARBONS IN ALGAE, BACTERIA, AND IN A RECENT LAKE SEDIMENT: A PRELIMINARY REPORT

    E-Print Network [OSTI]

    Han, Jerry; McCarthy, E.D.; Van Hoeven Jr., William; Calvin, Melvin; Bradley, W. H.

    2008-01-01

    ALIPHATIC HYDROCARBONS IN ALGAE, BACTERIA, AND IN A RECENTH F A PRELIMINARY REPORT IN ALGAE, BACTERIA, AKD IN A RECENTrests on the finding that algae have less cellulose and a

  4. A reliable method for the selection and confirmation of transconjugants of plant growth-promoting bacteria especially plant-associated Burkholderia spp.

    E-Print Network [OSTI]

    2015-01-01

    pUTgfp2x on LB. D. Agrobacterium tumefaciens Ca18 carryingtools for tagging Gram-negative bacteria with mCherry fora marker for detection of bacteria in environmental samples.

  5. Transport of bacteria and colloids in intermittent sand filters Maria Auset1, Arturo A. Keller1, Franois Brissaud2, Valentina Lazarova3

    E-Print Network [OSTI]

    Keller, Arturo A.

    Transport of bacteria and colloids in intermittent sand filters Maria Auset1, Arturo A. Keller1 of bacteria, the effects of cyclic infiltration and draining events (transient unsaturated flow) were percolating in a single pass through the unsaturated porous medium. Bacteria suspension Wastewater solution

  6. Development of a real-time PCR method for the detection of fossil 16S rDNA fragments of phototrophic sulfur bacteria

    E-Print Network [OSTI]

    Gilli, Adrian

    of phototrophic sulfur bacteria in the sediments of Lake Cadagno D. F. RAVASI,1 S. PEDUZZI,1 , 2 V. GUIDI,2 , 3 R sulfur bacteria in the chemocline has been monitored since 1994 with molecular methods such as 16S r sulfur bacteria populations from sediment samples. We detected fossil 16S rDNA of nine populations

  7. Single-photon ultrashort-lived radionuclides: symposium proceedings

    SciTech Connect (OSTI)

    Paras, P.; Thiessen, J.W. (eds.)

    1985-01-01

    The purpose was to define the current role and state-of-the-art regarding the development, clinical applications, and usefulness of generator-produced single-photon ultrashort-lived radionuclides (SPUSLR's) and to predict their future impact on medicine. Special emphasis was placed on the generator production of iridium-191, gold-195, and krypton-81. This report contains expanded summaries of the included papers. (ACR)

  8. ClimateChangeLIVE Webcast: Join the Climate Conversation

    Broader source: Energy.gov [DOE]

    Join ClimateChangeLIVE's webcast, bringing together students and climate experts for a discussion about climate change and what students and classes around the country are doing to be part of the climate solution. Students will be able to interact with climate scientists and experts online through Facebook and Twitter. A GreenWorks! grant will be offered to help schools with climate action projects.

  9. The problem of living in a world contaminated with chemicals

    SciTech Connect (OSTI)

    Metcalf, R.L.

    1990-12-31

    The proliferation of xenobiotic chemicals in the global environment poses living problems for each of us aboard {open_quotes}spaceship earth.{close_quotes} Seven case studies are presented that illustrate the magnitude of the problem that can result from waiting to identify toxic hazards until there have been decades of {open_quotes}human guinea pig{close_quotes} exposure. 25 refs., 5 tabs.

  10. Half-lives of Double $?^+$-decay with Two Neutrinos

    E-Print Network [OSTI]

    Yuejiao Ren; Zhongzhou Ren

    2015-01-07

    Nuclear double $\\beta ^-$-decays with two neutrinos were observed for many years and a systematic law describing the relation between their half-lives and decay energies was also proposed recently [Phys. Rev. C89, 064603 (2014)]. However, double $\\beta ^+$-decay ($\\beta ^+\\beta^+)$ with emission of both two positrons and two neutrinos has not been observed up to date. In this article, we perform a systematic analysis on the candidates of double $\\beta ^+$-decay, based on the 2012 nuclear mass table. Eight nuclei are found to be the good candidates for double $\\beta ^+$-decay and their half-lives are predicted according to the generalization of the systematic law to double $\\beta ^+$-decay. As far as we know, there is no theoretical result on double $\\beta ^+$-decay of nucleus $^{154}Dy$ and our result is the first prediction on this nucleus. This is also the first complete research on eight double $\\beta ^+$-decay candidates based on the available data of nuclear masses. It is expected that the calculated half-lives of double $\\beta ^+$-decay in this article will be useful for future experimental search of double $\\beta ^+$-decay.

  11. Turning Bacteria into Fuel: Cyanobacteria Designed for Solar-Powered Highly Efficient Production of Biofuels

    SciTech Connect (OSTI)

    None

    2010-01-01

    Broad Funding Opportunity Announcement Project: ASU is engineering a type of photosynthetic bacteria that efficiently produce fatty acids—a fuel precursor for biofuels. This type of bacteria, called Synechocystis, is already good at converting solar energy and carbon dioxide (CO2) into a type of fatty acid called lauric acid. ASU has modified the organism so it continuously converts sunlight and CO2 into fatty acids—overriding its natural tendency to use solar energy solely for cell growth and maximizing the solar-to-fuel conversion process. ASU’s approach is different because most biofuels research focuses on increasing cellular biomass and not on excreting fatty acids. The project has also identified a unique way to convert the harvested lauric acid into a fuel that can be easily blended with existing transportation fuels.

  12. Collective motion and nonequilibrium cluster formation in colonies of gliding bacteria

    E-Print Network [OSTI]

    Fernando Peruani; Joern Starruss; Vladimir Jakovljevic; Lotte Sogaard-Andersen; Andreas Deutsch; Markus Bar

    2013-02-01

    We characterize cell motion in experiments and show that the transition to collective motion in colonies of gliding bacterial cells confined to a monolayer appears through the organization of cells into larger moving clusters. Collective motion by non-equilibrium cluster formation is detected for a critical cell packing fraction around 17%. This transition is characterized by a scale-free power-law cluster size distribution, with an exponent $0.88\\pm0.07$, and the appearance of giant number fluctuations. Our findings are in quantitative agreement with simulations of self-propelled rods. This suggests that the interplay of self-propulsion of bacteria and the rod-shape of bacteria is sufficient to induce collective motion.

  13. Impact of elevated nitrate on sulfate-reducing bacteria: A comparative study of Desulfovibrio vulgaris

    SciTech Connect (OSTI)

    He, Q.; He, Z.; Joyner, D.C.; Joachimiak, M.; Price, M.N.; Yang, Z.K.; Yen, H.-C. B.; Hemme, C. L.; Chen, W.; Fields, M.; Stahl, D. A.; Keasling, J. D.; Keller, M.; Arkin, A. P.; Hazen, T. C.; Wall, J. D.; Zhou, J.

    2010-07-15

    Sulfate-reducing bacteria have been extensively studied for their potential in heavy-metal bioremediation. However, the occurrence of elevated nitrate in contaminated environments has been shown to inhibit sulfate reduction activity. Although the inhibition has been suggested to result from the competition with nitrate-reducing bacteria, the possibility of direct inhibition of sulfate reducers by elevated nitrate needs to be explored. Using Desulfovibrio vulgaris as a model sulfate-reducing bacterium, functional genomics analysis reveals that osmotic stress contributed to growth inhibition by nitrate as shown by the upregulation of the glycine/betaine transporter genes and the relief of nitrate inhibition by osmoprotectants. The observation that significant growth inhibition was effected by 70 mM NaNO{sub 3} but not by 70 mM NaCl suggests the presence of inhibitory mechanisms in addition to osmotic stress. The differential expression of genes characteristic of nitrite stress responses, such as the hybrid cluster protein gene, under nitrate stress condition further indicates that nitrate stress response by D. vulgaris was linked to components of both osmotic and nitrite stress responses. The involvement of the oxidative stress response pathway, however, might be the result of a more general stress response. Given the low similarities between the response profiles to nitrate and other stresses, less-defined stress response pathways could also be important in nitrate stress, which might involve the shift in energy metabolism. The involvement of nitrite stress response upon exposure to nitrate may provide detoxification mechanisms for nitrite, which is inhibitory to sulfate-reducing bacteria, produced by microbial nitrate reduction as a metabolic intermediate and may enhance the survival of sulfate-reducing bacteria in environments with elevated nitrate level.

  14. Effects of UV Light Disinfection on Tetracycline Resistant Bacteria in Wastewater Effluents 

    E-Print Network [OSTI]

    Childress, Hannah

    2011-10-21

    species can utilize the mechanisms of photoreactivation or dark repair to become reactivated. Photoreactivation occurs in UV- A or visible light; it is hypothesized that pyrimidine dimers form a complex with a photoreactivating enzyme which can... OF UV LIGHT DISINFECTION ON TETRACYCLINE RESISTANT BACTERIA IN WASTEWATER EFFLUENTS A Thesis by HANNAH CHILDRESS Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements...

  15. Cycling of DOC and DON by Novel Heterotrophic and Photoheterotrophic Bacteria in the Ocean: Final Report

    SciTech Connect (OSTI)

    Kirchman, David L

    2008-12-09

    The flux of dissolved organic matter (DOM) through aquatic bacterial communities is a major process in carbon cycling in the oceans and other aquatic systems. Our work addressed the general hypothesis that the phylogenetic make-up of bacterial communities and the abundances of key types of bacteria are important factors influencing the processing of DOM in aquatic ecosystems. Since most bacteria are not easily cultivated, the phylogenetic diversity of these microbes has to be assessed using culture-independent approaches. Even if the relevant bacteria were cultivated, their activity in the lab would likely differ from that under environmental conditions. This project found variation in DOM uptake by the major bacterial groups found in coastal waters. In brief, the data suggest substantial differences among groups in the use of high and molecular weight DOM components. It also made key discoveries about the role of light in affecting this uptake especially by cyanobacteria. In the North Atlantic Ocean, for example, over half of the light-stimulated uptake was by the coccoid cyanobacterium, Prochlorococcus, with the remaining uptake due to Synechococcus and other photoheterotrophic bacteria. The project also examined in detail the degradation of one organic matter component, chitin, which is often said to be the second most abundant compound in the biosphere. The findings of this project contribute to our understanding of DOM fluxes and microbial dynamics supported by those fluxes. It is possible that these findings will lead to improvements in models of the carbon cycle that have compartments for dissolved organic carbon (DOC), the largest pool of organic carbon in the oceans.

  16. A Scanning Auger Microprobe analysis of corrosion products associated with sulfate reducing bacteria

    SciTech Connect (OSTI)

    Sadowski, R.A.; Chen, G.; Clayton, C.R.; Kearns, J.R.; Gillow, J.B.; Francis, A.J.

    1995-03-01

    A Scanning Auger Microprobe analysis was performed on the corrosion products of an austenitic AISI type 304 SS after a potentiostatic polarization of one volt for ten minutes in a modified Postgate`s C media containing sulfate reducing bacteria. The corrosion products were characterized and mapped in local regions where pitting was observed. A critical evaluation of the applicability of this technique for the examination of microbially influenced corrosion (MIC) is presented.

  17. Environmental diagnostic analysis of ground water bacteria and their involvement in utilization of aromatic compounds

    SciTech Connect (OSTI)

    Wear, J.E. Jr.

    1993-05-01

    The objective of this study was to examine the hypothesis that select functional groups of bacteria from pristine sites have an innate ability to degrade synthetic aromatics that often contaminate groundwater environments,due to exposure to naturally occurring recalcitrant aromatics in their environment. This study demonstrates that subsurface microbial communities are capable of utilizing lignin and humic acid breakdown products. Utilizers of these compounds were found to be present in most all the wells tested. Even the deepest aquifer tested had utilizers present for all six of the aromatics tested. Highest counts for the aromatics tested were observed with the naturally occurring breakdown products of either lignin or humic acid. Carboxylic acids were found to be an important sole carbon source for groundwater bacteria possibly explained by the fact that they are produced by the oxidative cleavage of aromatic ring structures. The carbohydrate sole carbon sources that demonstrated the greatest densities were ones commonly associated with humics. This study indicates that utilization of naturally occurring aromatic compounds in the subsurface is an important nutritional source for groundwater bacteria. In addition, it suggests that adaptation to naturally occurring recalcitrant substrates is the origin of degradative pathways for xenobiotic compounds with analogous structure. This work has important implications for in situ bioremediation as a method of environmental cleanup.

  18. Identification of novel antimicrobials using a live-animal infection model

    E-Print Network [OSTI]

    Ausubel, Frederick M.

    pathogens points to the need for novel therapeutic approaches to combat infection. To discover novel nematode Caenorhabditis elegans infected with the human opportunistic pathogen Enterococcus faecalis. E the overmining of culti- vable microorganisms (11), a high background of toxic compounds or compounds with poor

  19. Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals

    DOE Patents [OSTI]

    Gambhir; Sanjiv (Portola Valley, CA), Pritha; Ray (Mountain View, CA)

    2009-04-28

    Novel double and triple fusion reporter gene constructs harboring distinct imageable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

  20. TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing...

    Office of Environmental Management (EM)

    ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's Oil Dependence Through Innovation TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's...