Sample records for bacteria microorganisms living

  1. Method of separating bacteria from free living amoebae

    DOE Patents [OSTI]

    Tyndall, Richard L. (Clinton, TN)

    1994-01-01T23:59:59.000Z

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  2. The isolation and evaluation of endophytic bacteria from live oaks as potential biological control agents for oak wilt in Texas

    E-Print Network [OSTI]

    Brooks, David Stewart

    1989-01-01T23:59:59.000Z

    on in vitro inhibition data, six isolates, three Gram-positive and three Gram-negative, were selected for colonization studies in container-grown red (()nereus texana) and live oak trees. Antibiotic resistant segregants, which allowed isolation on selective... in container-grown oak trees. Two experiments (Exp. i and II) were conducted with live oaks and one experiment with red oaks. In Exp. I, P. denitrificans significantly reduced the number of live oak trees expressing disease symptoms, while P. lucida showed...

  3. Bacteria-Mineral Interactions on the Surfaces of Metal-Resistant Bacteria

    SciTech Connect (OSTI)

    Malkin, A J

    2010-03-24T23:59:59.000Z

    The extraordinary ability of indigenous microorganisms, like metal-resistant bacteria, for biotransformation of toxic compounds is of considerable interest for the emerging area of environmental bioremediation. However, the underlying mechanisms by which metal-resistant bacteria transform toxic compounds are currently unknown and await elucidation. The project's objective was to study stress-induced responses of metal-resistant bacteria to environmental changes and chemical stimulants. This project involved a multi-institutional collaboration of our LLNL group with the group of Dr. H.-Y. Holman (Lawrence Berkeley National Laboratory). In this project, we have utilized metal-resistant bacteria Arthrobacter oxydans as a model bacterial system. We have utilized atomic force microscopy (AFM) to visualize for the first time at the nanometer scale formation of stress-induced structures on bacterial surfaces in response to Cr (VI) exposure. We have demonstrated that structure, assembly, and composition of these stress-induced structures are dependent on Cr (VI) concentrations. Our AFM observations of the appearance and development of stress-induced layers on the surfaces of Arthrobacter oxydans bacteria exposed to Cr (VI) were confirmed by Dr. Holman's biochemical, electron microscopy, and synchrotron infrared spectromicroscopy studies. In general, in vitro imaging of live microbial and cellular systems represents one of the most challenging issues in application of AFM. Various approaches for immobilization of bacteria on the substrate for in vitro imaging were tested in this project. Imaging of live bacteria was achieved, however further optimization of experimental methods are needed for high-resolution visualization of the cellular environmental structural dynamics by AFM. This project enhanced the current insight into molecular architecture, structural and environmental variability of bacterial systems. The project partially funded research for two book chapters (1,2), and we anticipate one more publication (3). The publications describe development of methods and results of studies of structural dynamics of metal-resistant bacteria that contribute to more comprehensive understanding of the architecture, function, and environmental dynamics of bacterial and cellular systems. The results of this LDRD were presented in invited talks and contributed presentations at five national and international conferences and five seminar presentations at the external institutions. These included invited talks at the conferences of Gordon Research, Materials Research and American Chemical Societies. Our scientific results and methodologies developed in this project enabled us to receive new funding for the multiyear project 'Chromium transformation pathways in metal-reducing bacteria' funded by the University of California Lab Fees Program ($500,000, 5/1/09 - 4/30/2012), with our proposal being ranked 1st from a total of 138 in the Earth, Energy, Environmental & Space Sciences panel.

  4. Microorganisms for producing organic acids

    DOE Patents [OSTI]

    Pfleger, Brian Frederick; Begemann, Matthew Brett

    2014-09-30T23:59:59.000Z

    Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.

  5. Response of Prochlorococcus ecotypes to co-culture with diverse marine bacteria

    E-Print Network [OSTI]

    Sher, Daniel

    Interactions between microorganisms shape microbial ecosystems. Systematic studies of mixed microbes in co-culture have revealed widespread potential for growth inhibition among marine heterotrophic bacteria, but similar ...

  6. arXiv:0812.2887v1[cond-mat.soft]15Dec2008 The hydrodynamics of swimming microorganisms

    E-Print Network [OSTI]

    Podgornik, Rudolf

    microorganisms move are familiar. Bacteria such as Escherichia coli detect gradients in nutrients and move, and the marine life ecosystem. Here we review the biophysical and mechanical principles of locomotion

  7. A laboratory study of the activities of dried rumen microorganisms

    E-Print Network [OSTI]

    Harbers, Leniel Henry

    1958-01-01T23:59:59.000Z

    . cal pro? ceases vere made in this study to determine the ~ ~ activity of dried rumen microorganisms. . 'ellulose d1- gestion vas studied by employing a yurified cellulose preparation as a substrate and determination of the changes in concentration... the conditions of these experiments. There vere~ hovevsr~ bacteria present in the dr1ed rumen preparations vhich vere capable of hydro- lysing carboxymethyl cellulose. A method of detsrming "cellulose d1gestion" using a soluble cellulose derivative as a...

  8. Introduction Many microorganisms including fungi and bacteria had been

    E-Print Network [OSTI]

    Qin, Wensheng

    and other plant cell wall fibers. In nature, degradation of cellulosic biomass is performed by mixtures, EGs) and exo-acting (cellobiohydrolases, CBH) enzymes, which act in a synergistic manner in biomass was targeted, (2) assays in which the reduction in substrate quantity was monitored, and (3) assays in which

  9. The hydrodynamics of swimming microorganisms This article has been downloaded from IOPscience. Please scroll down to see the full text article.

    E-Print Network [OSTI]

    Lauga, Eric

    . The reasons microorganisms move are familiar. Bacteria such as Escherichia coli detect gradients in nutrients processes, including reproduction, infection and the marine life ecosystem. Here we review the biophysical

  10. Study of Midgut Bacteria in the Red Imported Fire Ant, Solenopsis invicta Büren (Hymenoptera: Formicidae) 

    E-Print Network [OSTI]

    Medina, Freder

    2011-08-08T23:59:59.000Z

    Ants are capable of building close associations with plants, insects, fungi and bacteria. Symbionts can provide essential nutrients to their insect host, however, the development of new molecular tools has allowed the discovery of new microorganisms...

  11. Bacteria Marinobacter aquaeolei. Community Living. Microbes living in coral provide

    E-Print Network [OSTI]

    marine microbes are unable to make their own food; these heterotrophs sur- vive by absorbing organic are single-celled organisms that come in a variety of shapes and sizes. They have no clearly defined nucleus remaining unicellular organisms and all visible forms of life are termed Eukarya (or "Eukaryotes

  12. Bioelectricity Aware of bacteria

    E-Print Network [OSTI]

    Lovley, Derek

    (translation by Google Translate) Bacteria of the genus Geobacter and electrical nanowires. #12;Bioelectricity Aware of bacteria Bacteria of the genus Geobacter carry out anaerobic respiration the mechanism that makes these bacteria conductors of electricity. Researchers have studied

  13. A combined microfluidic/dielectrophoretic microorganism concentrator

    E-Print Network [OSTI]

    Gadish, Nitzan

    2005-01-01T23:59:59.000Z

    This thesis presents the development of a high-throughput microfluidic microorganism concentrator for pathogen detection applications. Interdigitated electrodes lining the bottom of the channel use positive dielectrophoretic ...

  14. Oil Production by a Consortium of Oleaginous Microorganisms grown on primary effluent wastewater

    SciTech Connect (OSTI)

    Hall, Jacqueline; Hetrick, Mary; French, Todd; Hernandez, Rafael; Donaldson, Janet; Mondala, Andro; Holmes, William

    2011-01-01T23:59:59.000Z

    Municipal wastewater could be a potential growth medium that has not been considered for cultivating oleaginous microorganisms. This study is designed to determine if a consortium of oleaginous microorganism can successfully compete for carbon and other nutrients with the indigenous microorganisms contained in primary effluent wastewater. RESULTS: The oleaginous consortium inoculated with indigenous microorganisms reached stationary phase within 24 h, reaching a maximum cell concentration of 0.58 g L -1. Water quality post-oleaginous consortium growth reached a maximum chemical oxygen demand (COD) reduction of approximately 81%, supporting the consumption of the glucose within 8 h. The oleaginous consortium increased the amount of oil produced per gram by 13% compared with indigenous microorganisms in raw wastewater. Quantitative polymerase chain reaction (qPCR) results show a substantial population increase in bacteria within the first 24 h when the consortium is inoculated into raw wastewater. This result, along with the fatty acid methyl esters (FAMEs) results, suggests that conditions tested were not sufficient for the oleaginous consortium to compete with the indigenous microorganisms.

  15. Leachability of salmonella and fecal pollution indicator bacteria through soil 

    E-Print Network [OSTI]

    Fehrmann, Robert Clinton

    1977-01-01T23:59:59.000Z

    of Department (Member) / /. ' Member) August 1977 74M3- ABSTRACT Leachability of Salmonella and Fecal Pollution Indicator Bacteria through Soil. (August 1977) Robert Clinton Fehrmann, B. S. , Texas AM University Chairman of Advisory Committee: Dr... microorganisms to be leached through soil, columns :ere filled with different soils and inoculs. ted with suspensions of fecal bacteria. Dif er ences in bacterial movement within a particular sni I, and bacterial movement between different types of soils...

  16. Cellulase producing microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig (Lenoir City, TN)

    1997-01-01T23:59:59.000Z

    Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  17. Cellulase producing microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1997-12-30T23:59:59.000Z

    Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  18. Scientists capture 'redox moments' in living cells | EMSL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    moments' in living cells Better understanding of hardy bacteria enhances tool for biofuel creation Scientists have charted a significant signaling network in a tiny organism...

  19. Bacteriocins of Aquatic Microorganisms and Their Potential

    E-Print Network [OSTI]

    Riley, Margaret

    , known as pyocins (Govan & Harris, 1985). In contrast, only 15-50% of Escherichia coli produce from marine microorganisms. These toxins have received far less attention than bacteriocins produced

  20. Living Sustainably

    E-Print Network [OSTI]

    Milbrath, Lester W.

    1998-01-01T23:59:59.000Z

    sustainable society does something more than keep people alive; livingsustainable modes of behavior that also lead to quality in living.

  1. Bacteria isolated from amoebae/bacteria consortium

    DOE Patents [OSTI]

    Tyndall, Richard L. (Clinton, TN)

    1995-01-01T23:59:59.000Z

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  2. Bacteria isolated from amoebae/bacteria consortium

    DOE Patents [OSTI]

    Tyndall, R.L.

    1995-05-30T23:59:59.000Z

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  3. Effects of microorganisms growth on the long-term stability of cement and bitumen

    SciTech Connect (OSTI)

    Libert, M.F.; Sellier, R.; Jouquet, G.; Trescinski, M.; Spor, H. [Nuclear Research Center of Cadarache, St.Paul-Lez-Durance (France)

    1993-12-31T23:59:59.000Z

    Cement is used as a coating matrix for nuclear waste or as an engineered barrier of waste repositories situated in geological formations. The effect of mineral acids excreted by bacteria (Thiobacillus) or organic acids produced by fungi, on the biodegradation of cement is discussed. Organic acids are quantitatively and qualitatively determined during growth of fungi over a two-year period. Even with high pH conditions, pH of the cement {approx} 11, growth of microorganisms occurs. Biodeterioration of cement is expressed in terms of bioleaching velocity of calcium and is observed by electron microscopy. Bitumen is commonly used as a matrix for the long-term storage of radioactive wastes. Long-term biodegrability of bitumen is discussed as a function of its chemical composition and various studied microorganisms.

  4. Recombinant microorganisms for increased production of organic acids

    DOE Patents [OSTI]

    Yi, Jian; Kleff, Susanne; Guettler, Michael V

    2013-04-30T23:59:59.000Z

    Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

  5. Recombinant microorganisms for increased production of organic acids

    DOE Patents [OSTI]

    Yi, Jian (East Lansing, MI); Kleff, Susanne (East Lansing, MI); Guettler, Michael V. (Holt, MI)

    2012-02-21T23:59:59.000Z

    Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

  6. Extremophilic iron-reducing bacteria: Their implications for possible life in extraterrestrial environments

    SciTech Connect (OSTI)

    Zhou, J.; Liu, S.V.; Zhang, C.; Palumbo, A.V.; Phelps, T.J.

    1998-06-01T23:59:59.000Z

    Iron reduction is believed to be an early form of respiration and iron-reducing bacteria might have evolved very early on Earth. To support this hypothesis, the authors began to search for both thermophilic and psychrophilic iron-reducing bacteria because iron-reducing capacity may be a widely distributed trait if ancestral microorganisms include extremophilic iron-reducing bacteria. To date, they have obtained thermophilic Fe(III)-reducing and magnetite-forming enrichment cultures from geologically and hydrologically isolated, millions of years-old deep terrestrial subsurface samples. Three dominant bacteria were identified based on 16S ribosomal RNA gene sequences. Phylogenetical analysis indicated that these bacteria were closely related to Thermoanaerobacter ethanoliticus. Two pure thermophilic iron-reducing bacteria have been isolated and characterized from these enrichments, they also are able to degrade cellulose and xylan. Geological evidence indicated that these bacteria were separated from modern organisms for about 200 million years, and they are the oldest isolated bacteria available now. Evolutionary sequence analysis showed that the 16S rRNA genes evolved extremely slowly in these bacteria. In addition, the authors have obtained about 30 psychrophilic iron-reducing bacteria in samples from Siberia and Alaska permafrost soils, Pacific marine sediments and Hawaii deep sea water. These bacteria were also able to reduce other heavy metals. The isolation of both thermophilic and psychrophilic iron-reducing bacteria from surface and subsurface environments has significant implications for microbial evolution and for studying the origin of life in extraterrestrial environments.

  7. Bacteria TMDL Projects 

    E-Print Network [OSTI]

    Wythe, Kathy

    2007-01-01T23:59:59.000Z

    of the projects are listed below. ? Peach CreekWater Quality Improvement Project ? Monitoring and Educational Programs Focused on Bacteria and Nutrient Runoff on Dairy Operations in the LeonWatershed ? Development of the Plum CreekWPP ? Impact of Proper... Star Healthy Streams * ? Environmental Management of Grazing Lands * *TWRI-managed projects More information on the initiative is available at www.tsswcb.state.tx.us/managementprogram/ initiatives/bacteria. Bacteria Projects Across the State...

  8. The effect of agrochemicals on indicator bacteria densities in outdoor mesocosmsemi_2287 3150..3158

    E-Print Network [OSTI]

    Rohr, Jason

    The effect of agrochemicals on indicator bacteria densities in outdoor mesocosmsemi_2287 3150, including patho- gens. Agrochemicals may influence the survival of these microorganisms in water bodies were used to investigate the response of Escherichia coli and enterococci to agrochemicals. Replicate

  9. Bacteria TMDL Projects

    E-Print Network [OSTI]

    Wythe, Kathy

    2007-01-01T23:59:59.000Z

    of TMDL projects for water bodies where swimming or wading may be unsafe or harvesting of oysters is limited or prohibited due to high concentrations of bacteria. ? Atascosa River: A TMDL Project for Bacteria ? Buffalo andWhite Oak Bayous: A TMDL...

  10. Bacteria to the Rescue | EMSL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bacteria to the Rescue Bacteria to the Rescue Released: December 05, 2011 High-performance computing adds speed, clarity to uranium bioremediation research Isosurfaces for aqueous...

  11. Storing data encoded DNA in living organisms

    DOE Patents [OSTI]

    Wong; Pak C. (Richland, WA), Wong; Kwong K. (Sugar Land, TX), Foote; Harlan P. (Richland, WA)

    2006-06-06T23:59:59.000Z

    Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

  12. Engineered microorganisms capable of producing target compounds under anaerobic conditions

    DOE Patents [OSTI]

    Buelter, Thomas (Denver, CO); Meinhold, Peter (Denver, CO); Feldman, Reid M. Renny (San Francisco, CA); Hawkins, Andrew C. (Parker, CO); Urano, Jun (Irvine, CA); Bastian, Sabine (Pasadena, CA); Arnold, Frances (La Canada, CA)

    2012-01-17T23:59:59.000Z

    The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

  13. Bacteria in shear flow

    E-Print Network [OSTI]

    Marcos, Ph.D. Massachusetts Institute of Technology

    2011-01-01T23:59:59.000Z

    Bacteria are ubiquitous and play a critical role in many contexts. Their environment is nearly always dynamic due to the prevalence of fluid flow: creeping flow in soil, highly sheared flow in bodily conduits, and turbulent ...

  14. Apparatus and method for the desulfurization of petroleum by bacteria

    DOE Patents [OSTI]

    Lizama, H.M.; Scott, T.C.; Scott, C.D.

    1995-10-17T23:59:59.000Z

    A method is described for treating petroleum with anaerobic microorganisms acting as biocatalysts that can remove sulfur atoms from hydrocarbon molecules, under anaerobic conditions, and then convert the sulfur atoms to hydrogen sulfide. The microorganisms utilized are from the family known as the ``Sulfate Reducing Bacteria``. These bacteria generate metabolic energy from the oxidation of organic compounds, but use oxidized forms of sulfur as an electron acceptor. Because the biocatalyst is present in the form of bacteria in an aqueous suspension, whereas the reacting substrate consists of hydrocarbon molecules in an organic phase, the actual desulfurization reaction takes place at the aqueous-organic interphase. To ensure adequate interfacial contacting and mass transfer, a biphasic electrostatic bioreactor system is utilized. The bioreactor is utilized to disperse and recoalesce a biocatalyst contained in the aqueous liquid phase into the organic liquid phase containing the sulfur. High-intensity electrical fields rupture the aqueous drops into a plurality of microdroplets and induce continuous coalescence and redispersion as the microdroplets travel through the organic phase, thus increasing surface area. As the aqueous microdroplets progress through the organic phase, the biocatalyst then reacts with the sulfur to produce hydrogen sulfide which is then removed from the bioreactor. The organic liquid, now free of the sulfur, is ready for immediate use or further processing. 5 figs.

  15. Apparatus and method for the desulfurization of petroleum by bacteria

    DOE Patents [OSTI]

    Lizama, Hector M. (Knoxville, TN); Scott, Timothy C. (Knoxville, TN); Scott, Charles D. (Oak Ridge, TN)

    1995-01-01T23:59:59.000Z

    A method for treating petroleum with anaerobic microorganisms acting as biocatalysts that can remove sulfur atoms from hydrocarbon molecules, under anaerobic conditions, and then convert the sulfur atoms to hydrogen sulfide. The microorganisms utilized are from the family known as the "Sulfate Reducing Bacteria." These bacteria generate metabolic energy from the oxidation of organic compounds, but use oxidized forms of sulfur as an electron acceptor. Because the biocatalyst is present in the form of bacteria in an aqueous suspension, whereas the reacting substrate consists of hydrocarbon molecules in an organic phase, the actual desulfurization reaction takes place at the aqueous-organic interphase. To ensure adequate interfacial contacting and mass transfer, a biphasic electrostatic bioreactor system is utilized. The bioreactor is utilized to disperse and recoalesce a biocatalyst contained in the aqueous liquid phase into the organic liquid phase containing the sulfur. High-intensity electrical fields rupture the aqueous drops into a plurality of microdroplets and induce continuous coalescence and redispersion as the microdroplets travel through the organic phase, thus increasing surface area. As the aqueous microdroplets progress through the organic phase, the biocatalyst then reacts with the sulfur to produce hydrogen sulfide which is then removed from the bioreactor. The organic liquid, now free of the sulfur, is ready for immediate use or further processing.

  16. Live Status

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh SchoolIn12electron 9 5Let us countLightingFebruaryandFor Users Live

  17. Cargo delivery into gram-negative bacteria via enterobactin uptake machinery

    E-Print Network [OSTI]

    Zheng, Tengfei

    2014-01-01T23:59:59.000Z

    Chapter 1. Introduction to Iron Homeostasis and Siderophores Iron is an essential nutrient for almost all living organisms. This Chapter presents an overview of iron homeostasis in human and bacteria, as well as the biology ...

  18. Evaluation of terrestrial microcosms for assessing the fate and effects of genetically engineered microorganisms on ecological processes

    SciTech Connect (OSTI)

    Fredrickson, J.K.; Bentjen, S.A.; Bolton, H. Jr.; Li, S.W.; Ligotke, M.W.; McFadden, K.M.; Van Voris, P.

    1989-04-01T23:59:59.000Z

    This project evaluates and modifies the existing US Environmental Protection Agency's Office of Pesticides and Toxic Substances (EPA/OPTS) terrestrial microcosm test system and test protocols such that they can be used to determine the environmental fate and ecological hazards of genetically engineered microorganisms (GEMs). The intact soil-core microcosm represents terrestrial ecosystems, and when coupled with appropriate test protocols, such microcosms may be appropriate to define and limit risks associated with the intentional release of GEMs. The terrestrial microcosm test system was used to investigate the survival and transport of two model GEMs (Azospirillum lipoferum and Pseudomonas sp. Tn5 mutants) to various trophic levels and niches and through intact soil cores. Subsequent effects on nutrient cycling and displacement of indigenous microorganisms were evaluated. The model organisms were a diazotrophic root-colonizing bacterium (A. lipoferum) and a wheat root growth-inhibiting rhizobacterium (Pseudomonas sp.). The transposable element Tn5 was used as a genetic marker for both microorganisms in two separate experiments. The organisms were subjected to transposon mutagenesis using a broad host-range-mobilizable suicide plasmid. The transposon Tn5 conferred levels of kanamycin resistance up to 500 ..mu..g/ml (Pseudomonas sp.), which allowed for selection of the bacteria from environmental samples. The presence of Tn5 DNA in the genome of the model GEMs also allowed the use of Tn5 gene probes to confirm and enumerate the microorganisms in different samples from the microcosms. Two types of root growth-inhibiting Pseudomonas sp. Tn5 mutants were obtained and used in microcosm studies: those that lacked the ability to inhibit either wheat root growth or the growth of other microorganisms in vitro (tox/sup /minus//) and those which retained these properties (tox/sup +/). 53 refs., 7 figs., 6 tabs.

  19. Laboratory and Field Evidence for Long-Term Starvation Survival of Microorganisms in Subsurface Terrestrial Environments

    SciTech Connect (OSTI)

    Kieft, T.L. [Biology Dept., New Mexico Inst. of Mining and Technology, Socorro, NM (United States); Murphy, E.M. [Pacific Northwest National Lab., Richland, WA (United States); Amy, P.S.; Haldeman, D.L. [Department of Biological Sciences, University of Nevada, Las Vegas, NV (United States); Ringelberg, D. B. [Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN (United States)]|[Oak Ridge National Lab., TN (United States)

    1997-12-31T23:59:59.000Z

    BIOGEOCHEMICAL MODELING OF GROUNDWATER FLOW AND NUTRIENT FLUX IN SUBSURFACE ENVIRONMENTS INDICATES THAT INHABITANT MICROORGANISMS EXPERIENCE SEVERE NUTRIENT LIMITATION. USING LABORATORY AND FIELD METHODS, WE HAVE BEEN TESTING STARVATION SURVIVAL IN SUBSURFACE MICROORGANISMS. IN MICROCOSM EXPERIMENTS, WE HAVE SHOWN THAT STRAINS OF TWO COMMONLY ISOLATED SUBSURFACE GENERA, ARTHROBACTER AND PSEUDOMONAS, ARE ABLE TO MAINTAIN VIABILITY IN LOW-NUTRIENT, NATURAL SUBSURFACE SEDIMENTS FOR OVER ONE YEAR. THESE NON-SPORE-FORMING BACTERIA UNDERGO RAPID INITIAL MINIATURIZATION FOLLOWED BY A STABILIZATION OF CELL SIZE. MEMBRANE LIPID PHOSPHOLIPID FATTY ACID (PLFA) PROFILES OF THE PSEUDOMONAS ARE CONSISTENT WITH ADAPTATION TO NUTRIENT STRESS; ARTHROBACTER APPARENTLY RESPONDS TO NUTRIENT DEPRIVATION WITHOUT ALTERING MEMBRANE PLFA. TO TEST SURVIVABILITY OF MICROORGANISMS OVER A GEOLOGIC TIME SCALE, WE CHARACTERIZED MICROBIAL COMMUNITIES IN A SEQUENCE OF UNSATURATED SEDIMENTS RANGING IN AGE FROM MODEM TO {gt}780,000 years. Sediments were relatively uniform silts in Eastern Washington State. Porewater ages at depth (measured by the chloride mass-balance approach) were as old as 3,600 years. Microbial abundance, biomass, and activities (measured by direct counts, culture counts, total PLFAs, and radiorespirometry) declined with sediment age. The pattern is consistent with laboratory microcosm studies of Microbial survival: rapid short-term change followed by long-term survival of a proportion of cells. Even the oldest sediments evinced a small but viable Microbial community. Microbial survival appeared to be a function of sediment age. Porewater age appeared to influence the markup of surviving communities, as indicated by PLFA profiles. Sites with different Porewater recharge rates and patterns of Pleistocene flooding had different communities.

  20. Effects of selected thermophilic microorganisms on crude oils at elevated temperatures and pressures. Final report

    SciTech Connect (OSTI)

    Premuzic, E.T.; Lin, M.S.

    1995-07-01T23:59:59.000Z

    During the past several years, a considerable amount of work has been carried out showing that microbially enhanced oil recovery (MEOR) is promising and the resulting biotechnology may be deliverable. At the Brookhaven National Laboratory (BNL), systematic studies have been conducted which dealt with the effects of thermophilic and thermoadapted bacteria on the chemical and physical properties of selected types of crude oils at elevated temperatures and pressures. Particular attention was paid to heavy crude oils from Venezuela, California, Alabama, Arkansas, Wyoming, Alaska, and other oil producing areas. Current studies indicate that during the biotreatment several chemical and physical properties of crude oils are affected. The oils are (1) emulsified; (2) acidified; (3) there is a qualitative and quantitative change in light and heavy fractions of the crudes; (4) there are chemical changes in fractions containing sulfur compounds; (5) there is an apparent reduction in the concentration of trace metals; (6) the qualitative and quantitative changes appear to be microbial species dependent; and (7) there is a distinction between {open_quotes}biodegraded{close_quotes} and {open_quotes}biotreated{close_quotes} oils. Preliminary results indicate the introduced microorganisms may become the dominant species in the bioconversion of oils. These studies also indicate the biochemical interactions between crude oils and microorganisms follow distinct trends, characterized by a group of chemical markers. Core-flooding experiments have shown significant additional crude oil recoveries are achievable with thermophilic microorganisms at elevated temperatures similar to those found in oil reservoirs. In addition, the biochemical treatment of crude oils has technological applications in downstream processing of crude oils such as in upgrading of low grade oils and the production of hydrocarbon based detergents.

  1. Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism

    DOE Patents [OSTI]

    Gaddy, J.L.; Clausen, E.C.

    1992-12-22T23:59:59.000Z

    A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H[sub 2]O and/or CO[sub 2] and H[sub 2] in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate. 3 figs.

  2. Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism

    DOE Patents [OSTI]

    Gaddy, James L. (Fayetteville, AR); Clausen, Edgar C. (Fayetteville, AR)

    1992-01-01T23:59:59.000Z

    A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H.sub.2 O and/or CO.sub.2 and H.sub.2 in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate.

  3. Glowing Bacteria: Transformation Efficiency

    E-Print Network [OSTI]

    Rose, Michael R.

    1 Glowing Bacteria: Transformation Efficiency Purpose: To determine how well your E. coli cells took up and expressed GFP after transformation. Background: Transformation efficiency is a quantitative)______ (Fraction of DNA spread on plate) = ______ µg plasmid DNA spread on plate #12;2 5. Transformation efficiency

  4. Fuel from Bacteria, CO2, Water, and Solar Energy: Engineering a Bacterial Reverse Fuel Cell

    SciTech Connect (OSTI)

    None

    2010-07-01T23:59:59.000Z

    Electrofuels Project: Harvard is engineering a self-contained, scalable Electrofuels production system that can directly generate liquid fuels from bacteria, carbon dioxide (CO2), water, and sunlight. Harvard is genetically engineering bacteria called Shewanella, so the bacteria can sit directly on electrical conductors and absorb electrical current. This current, which is powered by solar panels, gives the bacteria the energy they need to process CO2 into liquid fuels. The Harvard team pumps this CO2 into the system, in addition to water and other nutrients needed to grow the bacteria. Harvard is also engineering the bacteria to produce fuel molecules that have properties similar to gasoline or diesel fuel—making them easier to incorporate into the existing fuel infrastructure. These molecules are designed to spontaneously separate from the water-based culture that the bacteria live in and to be used directly as fuel without further chemical processing once they’re pumped out of the tank.

  5. Selective microorganism concentration using a dielectrophoresis-based microfabricated device

    E-Print Network [OSTI]

    Pucha?a, Katarzyna Anna

    2007-01-01T23:59:59.000Z

    Detection of pathogenic microorganisms is a significant challenge in medicine, environmental protection and biological threat safety because samples are often contaminated. This work presents a method of separating bacterial ...

  6. Microorganisms having enhanced tolerance to inhibitors and stress

    DOE Patents [OSTI]

    Brown, Steven D.; Yang, Shihui

    2014-07-29T23:59:59.000Z

    The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.

  7. Microorganisms having enhanced resistance to acetate and methods of use

    DOE Patents [OSTI]

    Brown, Steven D; Yang, Shihui

    2014-10-21T23:59:59.000Z

    The present invention provides isolated or genetically modified strains of microorganisms that display enhanced resistance to acetate as a result of increased expression of a sodium proton antiporter. The present invention also provides methods for producing such microbial strains, as well as related promoter sequences and expression vectors. Further, the present invention provides methods of producing alcohol from biomass materials by using microorganisms with enhanced resistance to acetate.

  8. activated sludge microorganisms: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Samples of wastewater were collected from Aslogy wastewater treatment plant, Zagazig, Egypt. The using bacteria embed on granular delivered as powder. Two runs of experimental...

  9. Field application of a genetically engineered microorganism for polycyclic aromatic hydrocarbon bioremediation process monitoring and control

    SciTech Connect (OSTI)

    Sayler, G.S.; Cox, C.D.; Ripp, S.; Nivens, D.E.; Werner, C.; Ahn, Y.; Matrubutham, U. [Univ. of Tennessee, Knoxville, TN (United States); Burlage, R. [Oak Ridge National Lab., TN (United States). Environmental Sciences Div.

    1998-11-01T23:59:59.000Z

    On October 30, 1996, the US Environmental Protection Agency (EPA) commenced the first test release of genetically engineered microorganisms (GEMs) for use in bioremediation. The specific objectives of the investigation were multifaceted and include (1) testing the hypothesis that a GEM can be successfully introduced and maintained in a bioremediation process, (2) testing the concept of using, at the field scale, reporter organisms for direct bioremediation process monitoring and control, and (3) acquiring data that can be used in risk assessment decision making and protocol development for future field release applications of GEMs. The genetically engineered strain under investigation is Pseudomonas fluorescens strain HK44 (King et al., 1990). The original P. fluorescens parent strain was isolated from polycyclic aromatic hydrocarbon (PAH) contaminated manufactured gas plant soil. Thus, this bacterium is able to biodegrade naphthalene (as well as other substituted naphthalenes and other PAHs) and is able to function as a living bioluminescent reporter for the presence of naphthalene contamination, its bioavailability, and the functional process of biodegradation. A unique component of this field investigation was the availability of an array of large subsurface soil lysimeters. This article describes the experience associated with the release of a genetically modified microorganism, the lysimeter facility and its associated instrumentation, as well as representative data collected during the first eighteen months of operation.

  10. Living a Sustainable Future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    solve the energy crisis through biological methods, including genetically engineering algae and cyanobacteria. Create a Sustainable Future: Living Living a Sustainable Future How...

  11. Evaluation of terrestrial microcosms for detection, fate, and survival analysis of genetically engineered microorganisms and their recombinant genetic material

    SciTech Connect (OSTI)

    Fredrickson, J.K.; Seidler, R.J.

    1989-02-01T23:59:59.000Z

    The research included in this document represents the current scientific information available regarding the applicability of terrestrial microcosms and related methodologies for evaluating detection methods and the fate and survival of microorganisms in the environment. The three terrestrial microcosms described in this document were used to evaluate the survival and fate of recombinant bacteria in soils and in association with plant surfaces and insects and their transport through soil with percolating water and root systems, and to test new methods and procedures to improve detection and enumeration of bacteria in soil. Simple (potting soil composed of peat mix and perlite, lacking environmental control and monitoring) and complex microcosms (agricultural soil with partial control and monitoring of environmental conditions) were demonstrated to be useful tools for preliminary assessments of microbial viability in terrestrial ecosystems. These studies evaluated the survival patterns of Enterobacter cloacae (pBR322) in soil and on plant surfaces and the ingestion of this same microorganism by cutworms and survival in the foregut and frass. The Versacore microcosm design was used to monitor the fate and competitiveness of genetically engineered bacteria in soil. Both selective media and gene probes were used successfully to follow the fate of two recombinant Pseudomonas sp. introduced into Versacore microcosms. Intact soil-core microcosms were employed to evaluate the fate and transport of genetically altered Azospirillum sp. and Pseudomonas sp. in soil and the plant rhizosphere. The usefulness of these various microcosms as a tool for risk assessment is underscored by the ease in obtaining soil from a proposed field release site to evaluate subsequent GEM fate and survival.

  12. Can entropy save bacteria?

    E-Print Network [OSTI]

    Suckjoon Jun

    2008-08-29T23:59:59.000Z

    This article presents a physical biology approach to understanding organization and segregation of bacterial chromosomes. The author uses a "piston" analogy for bacterial chromosomes in a cell, which leads to a phase diagram for the organization of two athermal chains confined in a closed geometry characterized by two length scales (length and width). When applied to rod-shaped bacteria such as Escherichia coli, this phase diagram predicts that, despite strong confinement, duplicated chromosomes will demix, i.e., there exists a primordial physical driving force for chromosome segregation. The author discusses segregation of duplicating chromosomes using the concentric-shell model, which predicts that newly synthesized DNA will be found in the periphery of the chromosome during replication. In contrast to chromosomes, these results suggest that most plasmids will be randomly distributed inside the cell because of their small sizes. An active partitioning system is therefore required for accurate segregation of low-copy number plasmids. Implications of these results are also sketched, e.g., on the role of proteins, segregation mechanisms for bacteria of diverse shapes, cell cycle of an artificial cell, and evolution.

  13. TSSWCB Bacteria-Related Projects 

    E-Print Network [OSTI]

    Wythe, Kathy

    2007-01-01T23:59:59.000Z

    of the projects are listed below. ? Peach CreekWater Quality Improvement Project ? Monitoring and Educational Programs Focused on Bacteria and Nutrient Runoff on Dairy Operations in the LeonWatershed ? Development of the Plum CreekWPP ? Impact of Proper... Star Healthy Streams * ? Environmental Management of Grazing Lands * *TWRI-managed projects More information on the initiative is available at www.tsswcb.state.tx.us/managementprogram/ initiatives/bacteria. Bacteria Projects Across the State...

  14. Studies on the activities of rumen microorganisms in vitro

    E-Print Network [OSTI]

    Johnson, Carl Edward

    1957-01-01T23:59:59.000Z

    STCDIES ON THE ACTIVITIES OF RUMEN MICROORGANISMS IN VITRO A Thesis Carl~Johnson Submitted to the, Gr;. dusts School of the Agricultural and Mec?anical Co13 cgs oi' Texas in partial fulfillment of the requirements for the degree of MAST'% OS... SCIENCE August 1)57 Major Subject: Animal Nutrition STUDIES CN THE ACTIVITIES OF RUNEN MICROORGANISMS IN VITRO A Thesis Carl Johnson Approved as to style and oontent b Cha of Committee Head of Department August 1957 ACIQIOWLEDGMENTS The author...

  15. Stringy and Membranic Theory of Swimming of Micro-organisms

    E-Print Network [OSTI]

    Masako Kawamura; Shin'ichi Nojiri; Akio Sugamoto

    1996-03-21T23:59:59.000Z

    When the swimming of micro-organisms is viewed from the string and membrane theories coupled to the velocity field of the fluid, a number of interesting results are derived; 1) importance of the area (or volume) preserving algebra, 2) usefulness of the $N$-point Reggeon (membranic) amplitudes, and of the gas to liquid transition in case of the red tide issues, 3) close relation between the red tide issue and the generation of Einstein gravity, and 4) possible understanding of the three different swimming ways of micro-organisms from the singularity structure of the shape space.

  16. TSSWCB Bacteria-Related Projects

    E-Print Network [OSTI]

    Wythe, Kathy

    2007-01-01T23:59:59.000Z

    of TMDL projects for water bodies where swimming or wading may be unsafe or harvesting of oysters is limited or prohibited due to high concentrations of bacteria. ? Atascosa River: A TMDL Project for Bacteria ? Buffalo andWhite Oak Bayous: A TMDL...

  17. Sampling for Bacteria in Wells

    E-Print Network [OSTI]

    Lesikar, Bruce J.

    2001-11-15T23:59:59.000Z

    Sampling for Bacteria in Wells E-126 11/01 Water samples for bacteria tests must always be col- lected in a sterile container. The procedure for collect- ing a water sample is as follows: 1. Obtain a sterile container from a Health Department...

  18. Introduction Microorganisms in sea ice function ecologically in the

    E-Print Network [OSTI]

    Stewart, Frank

    of the pack via brine drainage, ice melting, or ice break-up (Garrison & Buck 1989 and references thereinIntroduction Microorganisms in sea ice function ecologically in the Antarctic marine ecosystem where pelagic and sea ice habitats are intrinsically coupled (Laws 1985, Garrison 1991, Eicken 1992

  19. Engineering Biofuels from Photosynthetic Bacteria | Argonne National...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Engineering Biofuels from Photosynthetic Bacteria Technology available for licensing: Using photosynthetic bacteria to produce biofuels. 30-70% of the fuel's waste can be used to...

  20. Sampling for Bacteria in Wells 

    E-Print Network [OSTI]

    Lesikar, Bruce J.

    2001-11-15T23:59:59.000Z

    Sampling for Bacteria in Wells E-126 11/01 Water samples for bacteria tests must always be col- lected in a sterile container. The procedure for collect- ing a water sample is as follows: 1. Obtain a sterile container from a Health Department... immediately after collecting water sample. Refrigerate the sample and transport it to the laborato- ry (in an ice chest) as soon after collection as possible (six hours is best, but up to 30 hours). Many labs will not accept bacteria samples on Friday so check...

  1. Chromatic acclimation and population dynamics of green sulfur bacteria grown with spectrally tailored light

    E-Print Network [OSTI]

    Saikin, Semion K; Huh, Joonsuk; Hannout, Moataz; Wang, Yaya; Zare, Farrokh; Aspuru-Guzik, Alan; Tang, Joseph Kuo-Hsiang

    2014-01-01T23:59:59.000Z

    Living organisms have to adjust to their surrounding in order to survive in stressful conditions. We study this mechanism in one of most primitive creatures - photosynthetic green sulfur bacteria. These bacteria absorb photons very efficiently using the chlorosome antenna complexes and perform photosynthesis in extreme low-light environments. How the chlorosomes in green sulfur bacteria are acclimated to the stressful light conditions, for instance, if the spectrum of light is not optimal for absorption, is unknown. Studying Chlorobaculum tepidum cultures with far-red to near-infrared light-emitting diodes, we found that these bacteria react to changes in energy flow by regulating the amount of light-absorbing pigments and the size of the chlorosomes. Surprisingly, our results indicate that the bacteria can survive in near-infrared lights capturing low-frequency photons by the intermediate units of the light-harvesting complex. The latter strategy may be used by the species recently found near hydrothermal ve...

  2. Early Detection Saves Lives

    Broader source: Energy.gov [DOE]

    Former Department of Energy (DOE) workers tell how medical screening helped them lead healthier and longer lives.

  3. 2004 Sensory Transduction in Microorganisms Gordon Research Conference-January 11-16, 2004

    SciTech Connect (OSTI)

    Judith Armitage Carlyle Storm

    2005-01-07T23:59:59.000Z

    Research into the mechanisms involved in the sensing and responses of microorganisms to changes in their environment is currently very active in a large number of laboratories in the US, Europe, Japan, and Israel. A wide range of eukaryotic and prokaryotic species are being studies with regard to their sensing of chemical changes, light and redox signal and intercellular signaling, leading either to changes in motile behavior, gene expression or development. It has become increasingly apparent that the mechanisms involved in development have application in higher organisms while the sensing systems in bacteria are involved in a very wide range of physiological traits, from pathogenicity, through to biofilm formation. This is an area where a wide range of state of the art tools have been used and developed over the past few decades. Approaches include behavioral studies, electro-physiology, genetics, molecular biology, structural biology, biophysics and single molecule microscopy, immunocytochemistry and molecular and mathematical modeling, all of this helped by the large number of bacterial and eukaryotic microbial genome sequences now available. The central goal of this meeting is to bring together investigators using this wide range of approaches and different systems to compare data, share ideas and approaches and seeks to understand the fundamental principles underlying these responses.

  4. FUTURE LOGISTICS LIVING LABORATORY

    E-Print Network [OSTI]

    Heiser, Gernot

    FUTURE LOGISTICS LIVING LABORATORY Delivering Innovation The Future Logistics Living Lab that will provide logistics solutions for the future. The Living Lab is a demonstration, exhibition and work space by a group of logistics companies, research organisations, universities, and IT providers that includes NICTA

  5. Geobiology of marine magnetotactic bacteria

    E-Print Network [OSTI]

    Simmons, Sheri Lynn

    2006-01-01T23:59:59.000Z

    Magnetotactic bacteria (MTB) biomineralize intracellular membrane-bound crystals of magnetite (Fe3O4) or greigite (Fe3S4), and are abundant in the suboxic to anoxic zones of stratified marine environments worldwide. Their ...

  6. Aerobic microorganism for the degradation of chlorinated aliphatic hydrocarbons

    DOE Patents [OSTI]

    Fliermans, Carl B. (Augusta, GA)

    1989-01-01T23:59:59.000Z

    A chlorinated aliphatic hydrocarbon-degrading microorganism, having American Type Culture Collection accession numbers ATCC 53570 and 53571, in a biologically pure culture aseptically collected from a deep subsurface habitat and enhanced, mineralizes trichloroethylene and tetrachloroethylene to HCl, H.sub.2 O and Co.sub.2 under aerobic conditions stimulated by methane, acetate, methanol, tryptone-yeast extract, propane and propane-methane.

  7. Extraordinarily few organisms of a live recombinant BCG vaccine against tuberculosis induce maximal cell-mediated and protective immunity

    E-Print Network [OSTI]

    Horwitz, Marcus A; Harth, Günter; Dillon, Barbara Jane; Maslesa-Gali?, Sasa

    2006-01-01T23:59:59.000Z

    BCG vaccine by 0.7 logs in the lungs and 1.0 logs in the~10 live bacteria to the lungs of each animal, basedcounting primary lesions in the lungs of animals euthanized

  8. Results an data on the growth of the microorganisms

    SciTech Connect (OSTI)

    Laurinavichius, K.S.

    1995-10-09T23:59:59.000Z

    The study of biocorrosion of aluminum and its alloy was performed under conditions of continuous fermentation of thermophilic anaerobic microorganisms of different groups. This allowed us to examine the effect of various types of metabolic reactions of reduction-oxidation proceeding at different pH and temperatures under highly reduced conditions on aluminum corrosion. Besides, the experiments were performed where the part of the standard sample was exposed under strictly anaerobic conditions with an active microbiological process, and the second half was exposed under aerobic conditions. Thus the sample was exposed in gradient of oxidized-reduced conditions.

  9. Effects of remediation amendments on vadose zone microorganisms

    SciTech Connect (OSTI)

    Miller, Hannah M.; Tilton, Fred A.

    2012-08-10T23:59:59.000Z

    Surfactant-based foam delivery technology has been studied to remediate Hanford 200 area deep vadose zone sediment. However, the surfactants and remediation amendments have an unknown effect on indigenous subsurface microorganisms. Microbial populations are important factors to consider in remediation efforts due to their potential to alter soil geochemistry. This project focuses on measuring microbial metabolic responses to remediation amendments in batch and column studies using Deep Vadose Zone Sediments. Initial studies of the microbes from Hanford 200 area deep vadose zone sediment showed surfactants sodium dodecyl sulfate (SDS) and cocamidopropyl betaine (CAPB) and remediation amendment calcium polysulfide (CPS) had no affect on microbial growth using BiologTM Ecoplates. To move towards a more realistic field analog, soil columns were packed with Hanford 200 Area sediment. Once microbial growth in the column was verified by observing growth of the effluent solution on tryptic soy agar plates, remedial surfactants were injected into the columns, and the resulting metabolic diversity was measured. Results suggest surfactant sodium dodecyl sulfate (SDS) stimulates microbial growth. The soil columns were also visualized using X-ray microtomography to inspect soil packing and possibly probe for evidence of biofilms. Overall, BiologTM Ecoplates provide a rapid assay to predict effects of remediation amendments on Hanford 200 area deep vadose zone microorganisms.

  10. Methods for identifying an essential gene in a prokaryotic microorganism

    DOE Patents [OSTI]

    Shizuya, Hiroaki

    2006-01-31T23:59:59.000Z

    Methods are provided for the rapid identification of essential or conditionally essential DNA segments in any species of haploid cell (one copy chromosome per cell) that is capable of being transformed by artificial means and is capable of undergoing DNA recombination. This system offers an enhanced means of identifying essential function genes in diploid pathogens, such as gram-negative and gram-positive bacteria.

  11. Re-engineering bacteria for ethanol production

    DOE Patents [OSTI]

    Yomano, Lorraine P; York, Sean W; Zhou, Shengde; Shanmugam, Keelnatham; Ingram, Lonnie O

    2014-05-06T23:59:59.000Z

    The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.

  12. Sensory Transduction in Microorganisms 2008 Gordon Research Conference (January 2008)

    SciTech Connect (OSTI)

    Ann M. Stock

    2009-04-08T23:59:59.000Z

    Research into the mechanisms involved in the sensing and responses of microorganisms to changes in their environments is currently very active in a large number of laboratories worldwide. An increasingly wide range of prokaryotic and eukaryotic species are being studied with regard to their sensing of diverse chemical and physical stimuli, including nutrients, toxins, intercellular signaling molecules, redox indicators, light, pressure, magnetic fields, and surface contact, leading to adaptive responses affecting motile behavior, gene expression and/or development. The ease of manipulation of microorganisms has facilitated application of a broad range of techniques that have provided comprehensive descriptions of cellular behavior and its underlying molecular mechanisms. Systems and their molecular components have been probed at levels ranging from the whole organism down to atomic resolution using behavioral analyses; electrophysiology; genetics; molecular biology; biochemical and biophysical characterization; structural biology; single molecule, fluorescence and cryo-electron microscopy; computational modeling; bioinformatics and genomic analyses. Several model systems such as bacterial chemotaxis and motility, fruiting body formation in Myxococcus xanthus, and motility and development in Dictyostelium discoideum have traditionally been a focus of this meeting. By providing a basis for assessment of similarities and differences in mechanisms, understanding of these pathways has advanced the study of many other microbial sensing systems. This conference aims to bring together researchers investigating different prokaryotic and eukaryotic microbial systems using diverse approaches to compare data, share methodologies and ideas, and seek to understand the fundamental principles underlying sensory responses. Topic areas include: (1) Receptor Sensing and Signaling; (2) Intracellular Signaling (two-component, c-di-GMP, c-AMP, etc.); (3) Intracellular Localization and the Cytoskeleton; (4) Motors and Motility; (5) Differentiation and Development; (6) Host/Pathogen and Host/Symbiont Interactions; (7) Intercellular Communication; (8) Microbes and the Environment; and (9) Modeling Signaling Pathways.

  13. Visualizing a Living Building

    E-Print Network [OSTI]

    Padget, Steve

    2013-01-01T23:59:59.000Z

    This paper will chronicle the design process of the Odum School of Ecology at the University of Georgia and its objective to accomplish Living BuildingTM certification. In order to accomplish this, the architect (BNIM) and project partners applied...

  14. Next Generation Living 

    E-Print Network [OSTI]

    Vaughn, Caroline Elizabeth

    2013-02-06T23:59:59.000Z

    of mechanical systems, and our perceived outlook of sustainability can collaborate and aid each other toward sustainable architecture. This collaboration will take form through the proposal or a living and learning community for the students and faculty of Texas...

  15. Observation on growth of bacteria 

    E-Print Network [OSTI]

    Unknown

    2011-08-17T23:59:59.000Z

    5.16 Proposed copolymer from iptycene and fluorene............... 133 Figure 5.17 Perfluoropropylene trimers............................... 137 Figure 5.18 (a) Absorbance of 13 encapsulated in silica nanoparticles in solid state and (b... permeability, intracelular compartmentalization and photostability has made it dificult to extend the applications of probes from mamalian cels to plant cels, bacteria and yeast. 8 A careful examination of probe design with respect to their biocompatibility...

  16. Living a Sustainable Future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh SchoolIn12electron 9 5Let usNuclear SecurityTechnology |LivingLiving a

  17. Final Technical Report: Viral Infection of Subsurface Microorganisms and Metal/Radionuclide Transport

    SciTech Connect (OSTI)

    Weber, Karrie A.; Bender, Kelly S.; Li, Yusong

    2013-09-28T23:59:59.000Z

    Microbially mediated metabolisms have been identified as a significant factor either directly or indirectly impacting the fate and transport of heavy metal/radionuclide contaminants. To date microorganisms have been isolated from contaminated environments. Examination of annotated finished genome sequences of many of these subsurface isolates from DOE sites, revealed evidence of prior viral infection. To date the role that viruses play influencing microbial mortality and the resulting community structure which directly influences biogeochemical cycling in soils and sedimentary environments remains poorly understood. The objective of this exploratory study was to investigate the role of viral infection of subsurface bacteria and the formation of contaminant-bearing viral particles. This objective was approached by examining the following working hypotheses: (i) subsurface microorganisms are susceptible to viral infections by the indigenous subsurface viral community, and (ii) viral surfaces will adsorb heavy metals and radionuclides. Our results have addressed basic research needed to accomplish the BER Long Term Measure to provide sufficient scientific understanding such that DOE sites would be able to incorporate coupled physical, chemical and biological processes into decision making for environmental remediation or natural attenuation and long-term stewardship by establishing viral-microbial relationships on the subsequent fate and transport of heavy metals and radionuclides. Here we demonstrated that viruses play a significant role in microbial mortality and community structure in terrestrial subsurface sedimentary systems. The production of viral-like particles within subsurface sediments in response to biostimulation with dissolved organic carbon and a terminal electron acceptor resulted in the production of viral-like particles. Organic carbon alone did not result in significant viral production and required the addition of a terminal electron acceptor (nitrate), indicating that nutrients are not limiting viral production, but rather substrates that can be converted into energy for host metabolism. Our results also revealed that cell abundance was not correlated to the mineralization of organic carbon, but rather viruses were positively correlated with carbon mineralization. This is a result of viral-mediated cell lysis and demonstrates that viruses are sensitive indicators of microbial activity. Viruses as an indicator of microbial activity was not unique to batch culture studies as results obtained from an in situ field experiment conducted at the DOE Old Rifle Field site. This study revealed that viral abundance increased in response to the injection of oxygenated groundwater and influx of dissolved organic carbon whereas cell abundance changes were minimal. However, the extent to which viral-mediated cell lysis alters organic matter pools subsequently influencing microbial community structure and biogeochemical function remains a critical question in subsurface biogeochemical cycling. The production of significant numbers of viruses in groundwater has implications for nanoparticulate metal as well as carbon transport in groundwater. We have demonstrated that the virus surface is reactive and will adsorb heavy metals. Thus viruses can promote colloidal contaminant mobility. Interestingly, the presence of heavy metals has a positive effect on infectivity of the phage, increasing phage infection which could lead to further production of viruses. Together, the results indicate that the sorption of metals to the surface of viruses could not only contribute to nanoparticulate metal as well as carbon transport but could also enhance infectivity further contributing to cell lysis which could subsequently influence biogeochemical cycling. As more viruses infect host microbial populations the high concentration of metals would enhance infection, resulting in cell lysis, and decreasing the metabolically active host population while yielding greater numbers of viruses capable of transporting contaminats. Additional studie

  18. Transformation of gram positive bacteria by sonoporation

    DOE Patents [OSTI]

    Yang, Yunfeng; Li, Yongchao

    2014-03-11T23:59:59.000Z

    The present invention provides a sonoporation-based method that can be universally applied for delivery of compounds into Gram positive bacteria. Gram positive bacteria which can be transformed by sonoporation include, for example, Bacillus, Streptococcus, Acetobacterium, and Clostridium. Compounds which can be delivered into Gram positive bacteria via sonoporation include nucleic acids (DNA or RNA), proteins, lipids, carbohydrates, viruses, small organic and inorganic molecules, and nano-particles.

  19. Identification of subsurface microorganisms at Yucca Mountain; Fourth quarterly report

    SciTech Connect (OSTI)

    Stetzenbach, L.D.

    1993-08-01T23:59:59.000Z

    Bacteria isolated from water samples collected in a series of ground water springs have been isolated, enumerated, and identified from twenty six sites. Ten sites were sampled in Death Valley, California and sixteen sites were sampled in Ash Meadows, Nevada. Replicate samples were collected and tested from four locations. All water samples were collected in conjunction with the HRC chemistry group conducting ground water fingerprinting studies. The protocol for collection of samples, as described in the 3rd quarterly report, specified aseptic collection in sterile screw-capped containers and transportation on ice to the HRC microbiology laboratory. All samples were inoculated by spread plating onto R2A (Difco Laboratories, Detroit, MI) bacterial culture medium. the R2A plates were then incubated at 28{degrees} for 5--7 days and colonies wee counted with the aid of a grid template and magnifying lens.

  20. Spectroscopic diagnostics for bacteria in biologic sample

    DOE Patents [OSTI]

    El-Sayed, Mostafa A. (Atlanta, GA); El-Sayed, Ivan H. (Somerville, MA)

    2002-01-01T23:59:59.000Z

    A method to analyze and diagnose specific bacteria in a biologic sample using spectroscopy is disclosed. The method includes obtaining the spectra of a biologic sample of a non-infected patient for use as a reference, subtracting the reference from the spectra of an infected sample, and comparing the fingerprint regions of the resulting differential spectrum with reference spectra of bacteria in saline. Using this diagnostic technique, specific bacteria can be identified sooner and without culturing, bacteria-specific antibiotics can be prescribed sooner, resulting in decreased likelihood of antibiotic resistance and an overall reduction of medical costs.

  1. Managing Bacteria Pollution in Texas Waters

    E-Print Network [OSTI]

    Wythe, Kathy

    2007-01-01T23:59:59.000Z

    Story by Kathy Wythe tx H2O | pg. 2 BACTERIA MANAGING tx H2O | pg. 3 IN TEXAS WATERS POLLUTION Managing Bacteria Pollution in Texas Waters tx H2O | pg. 4 W ith 310 water bodies in Texas failing to meetwater quality standards because... of bacteria,managing bacteria pollution is commanding the attention of water agencies, researchers and stake- holders across Texas. These water bodies are listed in the 2006 Texas Water Quality Inventory and 303(d) List for failing to meet the standards...

  2. Managing Bacteria Pollution in Texas Waters 

    E-Print Network [OSTI]

    Wythe, Kathy

    2007-01-01T23:59:59.000Z

    Story by Kathy Wythe tx H2O | pg. 2 BACTERIA MANAGING tx H2O | pg. 3 IN TEXAS WATERS POLLUTION Managing Bacteria Pollution in Texas Waters tx H2O | pg. 4 W ith 310 water bodies in Texas failing to meetwater quality standards because... of bacteria,managing bacteria pollution is commanding the attention of water agencies, researchers and stake- holders across Texas. These water bodies are listed in the 2006 Texas Water Quality Inventory and 303(d) List for failing to meet the standards...

  3. Systematic characterization of protein glycosylation of bacteria...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bacteria cell Insoluble fraction Glycoprotein Enrichment Integrated top-down and bottom-up Glycoprotein & Glycopeptide Step 1: Glycoproteome profile Glycans HILIC-FTICR-MSMS...

  4. Assessing the transport and fate of bioengineered microorganisms in the environment

    SciTech Connect (OSTI)

    Barnthouse, L.W.; Palumbo, A.V.

    1985-01-01T23:59:59.000Z

    We review the methods currently available for quantifying the transport and fate of microbes in atmospheric and aqueous media and assess their adequacy for purposes of risk assessment. We review the literature on transport and fate of microorganisms, including studies of: (1) pathways of migration, (2) the survival of microorganisms during transport and fate. In addition, we review the transport and fate models that have been used in environmental risk assessments for radionuclides and toxic chemicals and evaluate their applicability to the problem of assessing environmental risks of bioengineered microorganisms.

  5. Beating bacteria: Scientists work to understand and track bacteria in water 

    E-Print Network [OSTI]

    Wythe, Kathy

    2010-01-01T23:59:59.000Z

    tx H2O | pg. 11 Story by Kathy Wythe Bacteria Task Force Recommendations Acknowledging the enormity of the bacteria problem within the state, in September 2006, TCEQ and the Texas State Soil and Water Conservation Board (TSSWCB...) established a joint Task Force on Bacteria Total Maximum Daily Loads (TMDLs) to make recommenda- tions to strengthen the agencies? efforts in cleaning up bacteria- contaminated waters. Dr. Allan Jones, formerly of the Texas Water Resources Institute...

  6. Metabolic Engineering and Modeling of Metabolic Pathways to Improve Hydrogen Production by Photosynthetic Bacteria

    SciTech Connect (OSTI)

    Jiao, Y. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Navid, A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2014-12-19T23:59:59.000Z

    Rising energy demands and the imperative to reduce carbon dioxide (CO2) emissions are driving research on biofuels development. Hydrogen gas (H2) is one of the most promising biofuels and is seen as a future energy carrier by virtue of the fact that 1) it is renewable, 2) does not evolve the “greenhouse gas” CO2 in combustion, 3) liberates large amounts of energy per unit weight in combustion (having about 3 times the energy content of gasoline), and 4) is easily converted to electricity by fuel cells. Among the various bioenergy strategies, environmental groups and others say that the concept of the direct manufacture of alternative fuels, such as H2, by photosynthetic organisms is the only biofuel alternative without significant negative criticism [1]. Biological H2 production by photosynthetic microorganisms requires the use of a simple solar reactor such as a transparent closed box, with low energy requirements, and is considered as an attractive system to develop as a biocatalyst for H2 production [2]. Various purple bacteria including Rhodopseudomonas palustris, can utilize organic substrates as electron donors to produce H2 at the expense of solar energy. Because of the elimination of energy cost used for H2O oxidation and the prevention of the production of O2 that inhibits the H2-producing enzymes, the efficiency of light energy conversion to H2 by anoxygenic photosynthetic bacteria is in principle much higher than that by green algae or cyanobacteria, and is regarded as one of the most promising cultures for biological H2 production [3]. Here implemented a simple and relatively straightforward strategy for hydrogen production by photosynthetic microorganisms using sunlight, sulfur- or iron-based inorganic substrates, and CO2 as the feedstock. Carefully selected microorganisms with bioengineered beneficial traits act as the biocatalysts of the process designed to both enhance the system efficiency of CO2 fixation and the net hydrogen production rate. Additionally we applied metabolic engineering approaches guided by computational modeling for the chosen model microorganisms to enable efficient hydrogen production.

  7. Origin and Phylogeny of Microbes Living in Permanent Antarctic Lake Ice

    E-Print Network [OSTI]

    Priscu, John C.

    Origin and Phylogeny of Microbes Living in Permanent Antarctic Lake Ice D. A. Gordon,1, * J. Priscu of bacteria and cyanobacteria colonizing sediment particles in the per- manent ice cover of an Antarctic lake collected from a depth of 2.5 m in the 4-m-thick ice cover of Lake Bonney, McMurdo Dry Valleys, Antarctica

  8. Aggregation Patterns in Stressed Bacteria

    SciTech Connect (OSTI)

    Tsimring, L.; Levine, H. [Institute for Nonlinear Science, University of California at San Diego, La Jolla, California 92093-0402 (United States)] [Institute for Nonlinear Science, University of California at San Diego, La Jolla, California 92093-0402 (United States); Aranson, I. [Deptartment of Physics, Bar-Ilan University, Ramat Gan (Israel)] [Deptartment of Physics, Bar-Ilan University, Ramat Gan (Israel); Ben-Jacob, E.; Cohen, I.; Shochet, O. [School of Physics and Astronomy, Raymond & Beverly Sackler Faculty of Exact Sciences, Tel-Aviv University, Tel-Aviv 69978 (Israel)] [School of Physics and Astronomy, Raymond & Beverly Sackler Faculty of Exact Sciences, Tel-Aviv University, Tel-Aviv 69978 (Israel); Reynolds, W.N. [Complex Systems Division, Los Alamos National Laboratory, Los Alamos New Mexico (United States)] [Complex Systems Division, Los Alamos National Laboratory, Los Alamos New Mexico (United States)

    1995-08-28T23:59:59.000Z

    We study the formation of spot patterns seen in bacterial colonies when the bacteria are subjected to oxidative stress due to hazardous by-products of respiration. The cell density is coupled to a chemoattractant concentration as well as to nutrient and waste fields. The model combines the propagation of a front of motile bacterial radially outward from an initial site, a Turing instability of the uniformly dense state, and a reduction of motility for cells sufficiently far behind the front. The wide variety of patterns seen in the experiments is reproduced by the model by varying the details of the initiation of the chemoattractant emission as well as the transition to a nonmotile phase.

  9. Method of dispersing a hydrocarbon using bacteria

    DOE Patents [OSTI]

    Tyndall, Richard L. (Clinton, TN)

    1996-01-01T23:59:59.000Z

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  10. Method of dispersing a hydrocarbon using bacteria

    DOE Patents [OSTI]

    Tyndall, R.L.

    1996-09-24T23:59:59.000Z

    A new protozoan derived microbial consortia and method for their isolation are provided. The isolated consortia and bacteria are useful for treating wastes such as trichloroethylene and trinitrotoluene. The isolated consortia, bacteria, and dispersants are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  11. Technical Note: Evaluation of Effective Microorganisms (EM) In Solid Waste Management

    E-Print Network [OSTI]

    Sekeran, V.; Balaji, C.; Bhagavathipushpa, T.

    2005-01-01T23:59:59.000Z

    Microorganisms (EM) In Solid Waste Management V. Sekeran C.is the treatment, disposal and/or recycling of solid wastes.Generally solid waste from a municipality consists of

  12. Chemotactic selection of pollutant degrading soil bacteria

    DOE Patents [OSTI]

    Hazen, T.C.

    1991-03-04T23:59:59.000Z

    A method is described for identifying soil microbial strains which may be bacterial degraders of pollutants. This method includes: Placing a concentration of a pollutant in a substantially closed container; placing the container in a sample of soil for a period of time ranging from one minute to several hours; retrieving the container and collecting its contents; microscopically determining the identity of the bacteria present. Different concentrations of the pollutant can be used to determine which bacteria respond to each concentration. The method can be used for characterizing a polluted site or for looking for naturally occurring biological degraders of the pollutant. Then bacteria identified as degraders of the pollutant and as chemotactically attracted to the pollutant are used to innoculate contaminated soil. To enhance the effect of the bacteria on the pollutant, nutrients are cyclicly provided to the bacteria then withheld to alternately build up the size of the bacterial colony or community and then allow it to degrade the pollutant.

  13. Nucleic acid molecules conferring enhanced ethanol tolerance and microorganisms having enhanced tolerance to ethanol

    DOE Patents [OSTI]

    Brown, Steven; Guss, Adam; Yang, Shihui; Karpinets, Tatiana; Lynd, Lee; Shao, Xiongjun

    2014-01-14T23:59:59.000Z

    The present invention provides isolated nucleic acid molecules which encode a mutant acetaldehyde-CoA/alcohol dehydrogenase or mutant alcohol dehydrogenase and confer enhanced tolerance to ethanol. The invention also provides related expression vectors, genetically engineered microorganisms having enhanced tolerance to ethanol, as well as methods of making and using such genetically modified microorganisms for production of biofuels based on fermentation of biomass materials.

  14. Anaerobic Redox Cycling of Iron by Freshwater Sediment Microorganisms

    SciTech Connect (OSTI)

    Weber, Karrie A.; Urrutia, Matilde M.; Churchill, Perry F.; Kukkadapu, Ravi K.; Roden, Eric E.

    2006-01-01T23:59:59.000Z

    The potential for microbially-mediated anaerobic redox cycling of iron (Fe) was examined in a first-generation enrichment culture of freshwater wetland sediment microorganisms. MPN enumerations revealed the presence of significant populations of Fe(III)-reducing (ca. 108 cells mL-1) and Fe(II)-oxidizing, nitrate-reducing organisms (ca. 105 cells mL-1) in the sediment used to inoculate the enrichment cultures. Nitrate reduction commenced immediately following inoculation of acetate-containing (ca. 1 mM) medium with a small quantity (1% vol/vol) of wetland sediment, and resulted in the transient accumulation of NO2- and production of a mixture of end-products including NH4+. Fe(III) oxide (high surface area goethite) reduction took place - after NO3- was depleted and continued until all the acetate was utilized. Addition of NO3 after Fe(III) reduction ceased resulted in the immediate oxidation of Fe(II) coupled to reduction of + NO3-to NH4 . No significant NO2- accumulation was observed during nitrate-dependent Fe(II) oxidation. No Fe(II) oxidation occurred in pasteurized controls. Microbial community structure in the enrichment was monitored by DGGE analysis of PCR amplified 16s rDNA and RT-PCR amplified 16S rRNA, as well as by construction of 16S rDNA clone libraries for four different time points during the experiment. Strong similarities in dominant members of the microbial community were observed in the Fe(III) reduction and nitrate-dependent Fe(II) oxidation phases of the experiment, specifically the common presence of organisms closely related (= 95% sequence similarity) to the genera Geobacter and Dechloromonas. These results indicate that the wetland sediments contained organisms such as Geobacter sp. which are capable of both + dissimilatory Fe(III) reduction and oxidation of Fe(II) with reduction of NO3-reduction to NH4 . Our findings suggest that microbially-catalyzed nitrate-dependent Fe(II) oxidation has the potential to contribute to a dynamic anaerobic Fe redox cycle in freshwater sediments.

  15. Recovery of uranium by using new microorganisms isolated from North American uranium deposits

    SciTech Connect (OSTI)

    Sakaguchi, T.; Nakajima, A.; Tsuruta, T. [Miyazaki Medical College (Japan)

    1995-12-31T23:59:59.000Z

    Some attempts were made to remove uranium that may be present in refining effluents, mine tailings by using new microorganisms isolated from uranium deposits and peculiar natural environments. To screen microorganisms isolated from uranium deposits and peculiar natural environments in North America and Japan for maximal accumulation of uranium, hundreds of microorganisms were examined. Some microorganisms can accumulate about 500 mg (4.2 mEq) of uranium per gram of Microbial cells within 1 h. The uranium accumulation capacity of the cells exceeds that of commercially available chelating agents (2-3 mEq/g adsorbent). We attempted to recover uranium from uranium refining waste water by using new microorganisms. As a result, these microbial cells can recover trace amounts of uranium from uranium waste water with high efficiency. These strains also have a high accumulating ability for thorium. Thus, these new microorganisms can be used as an adsorbing agent for the removal of nuclear elements may be present in metallurgical effluents, mine tailings and other waste sources.

  16. Living olefin polymerization processes

    DOE Patents [OSTI]

    Schrock, Richard R.; Bauman, Robert

    2006-11-14T23:59:59.000Z

    Processes for the living polymerization of olefin monomers with terminal carbon-carbon double bonds are disclosed. The processes employ initiators that include a metal atom and a ligand having two group 15 atoms and a group 16 atom or three group 15 atoms. The ligand is bonded to the metal atom through two anionic or covalent bonds and a dative bond. The initiators are particularly stable under reaction conditions in the absence of olefin monomer. The processes provide polymers having low polydispersities, especially block copolymers having low polydispersities. It is an additional advantage of these processes that, during block copolymer synthesis, a relatively small amount of homopolymer is formed.

  17. Living SafeLy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh SchoolIn12electron 9 5Let usNuclear SecurityTechnology |Living SafeLy

  18. Living With Wildfire

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh SchoolIn12electron 9 5Let usNuclear SecurityTechnology |Living

  19. Hydrogen (H2) Production by Anoxygenic Purple Nonsulfur Bacteria...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Anoxygenic Purple Nonsulfur Bacteria Hydrogen (H2) Production by Anoxygenic Purple Nonsulfur Bacteria Presentation by Jake McKinlay, Indiana University, at the Biological Hydrogen...

  20. How plants beckon bacteria that do it harm | EMSL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    plants beckon bacteria that do it harm How plants beckon bacteria that do it harm Released: April 28, 2014 Work on microbial signaling offers better biofuels, human health...

  1. Motility fractionation of bacteria by centrifugation

    E-Print Network [OSTI]

    Claudio Maggi; Alessia Lepore; Jacopo Solari; Alessandro Rizzo; Roberto Di Leonardo

    2013-10-10T23:59:59.000Z

    Centrifugation is a widespread laboratory technique used to separate mixtures into fractions characterized by a specific size, weight or density. We demonstrate that centrifugation can be also used to separate swimming cells having different motility. To do this we study self-propelled bacteria under the influence of an external centrifugal field. Using dynamic image correlation spectroscopy we measure the spatially resolved motility of bacteria after centrifugation. A significant gradient in swimming-speeds is observed for increasing centrifugal speeds. Our results can be reproduced by a model that treats bacteria as "hot" colloidal particles having a diffusion coefficient that depends on the swimming speed.

  2. Determination of kinetic coefficients for the simultaneous reduction of sulfate and uranium by Desulfovibrio desulfuricans bacteria

    SciTech Connect (OSTI)

    Tucker, M.D.

    1995-05-01T23:59:59.000Z

    Uranium contamination of groundwaters and surface waters near abandoned mill tailings piles is a serious concern in many areas of the western United States. Uranium usually exists in either the U(IV) or the U(VI) oxidation state. U(VI) is soluble in water and, as a result, is very mobile in the environment. U(IV), however, is generally insoluble in water and, therefore, is not subject to aqueous transport. In recent years, researchers have discovered that certain anaerobic microorganisms, such as the sulfate-reducing bacteria Desulfovibrio desulfuricans, can mediate the reduction of U(VI) to U(IV). Although the ability of this microorganism to reduce U(VI) has been studied in some detail by previous researchers, the kinetics of the reactions have not been characterized. The purpose of this research was to perform kinetic studies on Desulfovibrio desulficans bacteria during simultaneous reduction of sulfate and uranium and to determine the phase in which uranium exists after it has been reduced and precipitated from solution. The studies were conducted in a laboratory-scale chemostat under substrate-limited growth conditions with pyruvate as the substrate. Kinetic coefficients for substrate utilization and cell growth were calculated using the Monod equation. The maximum rate of substrate utilization (k) was determined to be 4.70 days{sup {minus}1} while the half-velocity constant (K{sub s}) was 140 mg/l COD. The yield coefficient (Y) was determined to be 0.17 mg cells/mg COD while the endogenous decay coefficient (k{sub d}) was calculated as 0.072 days{sup {minus}1}. After reduction, U(IV) Precipitated from solution in the uraninite (UO{sub 2}) phase. Uranium removal efficiency as high as 90% was achieved in the chemostat.

  3. Fuel from Bacteria: Bioconversion of Carbon Dioxide to Biofuels by Facultatively Autotrophic Hydrogen Bacteria

    SciTech Connect (OSTI)

    None

    2010-07-01T23:59:59.000Z

    Electrofuels Project: Ohio State is genetically modifying bacteria to efficiently convert carbon dioxide directly into butanol, an alcohol that can be used directly as a fuel blend or converted to a hydrocarbon, which closely resembles a gasoline. Bacteria are typically capable of producing a certain amount of butanol before it becomes too toxic for the bacteria to survive. Ohio State is engineering a new strain of the bacteria that could produce up to 50% more butanol before it becomes too toxic for the bacteria to survive. Finding a way to produce more butanol more efficiently would significantly cut down on biofuel production costs and help make butanol cost competitive with gasoline. Ohio State is also engineering large tanks, or bioreactors, to grow the biofuel-producing bacteria in, and they are developing ways to efficiently recover biofuel from the tanks.

  4. Comparative genomics of the lactic acid bacteria

    SciTech Connect (OSTI)

    Makarova, K.; Slesarev, A.; Wolf, Y.; Sorokin, A.; Mirkin, B.; Koonin, E.; Pavlov, A.; Pavlova, N.; Karamychev, V.; Polouchine, N.; Shakhova, V.; Grigoriev, I.; Lou, Y.; Rokhsar, D.; Lucas, S.; Huang, K.; Goodstein, D. M.; Hawkins, T.; Plengvidhya, V.; Welker, D.; Hughes, J.; Goh, Y.; Benson, A.; Baldwin, K.; Lee, J.-H.; Diaz-Muniz, I.; Dosti, B.; Smeianov, V,; Wechter, W.; Barabote, R.; Lorca, G.; Altermann, E.; Barrangou, R.; Ganesan, B.; Xie, Y.; Rawsthorne, H.; Tamir, D.; Parker, C.; Breidt, F.; Broadbent, J.; Hutkins, R.; O'Sullivan, D.; Steele, J.; Unlu, G.; Saier, M.; Klaenhammer, T.; Richardson, P.; Kozyavkin, S.; Weimer, B.; Mills, D.

    2006-06-01T23:59:59.000Z

    Lactic acid-producing bacteria are associated with various plant and animal niches and play a key role in the production of fermented foods and beverages. We report nine genome sequences representing the phylogenetic and functional diversity of these bacteria. The small genomes of lactic acid bacteria encode a broad repertoire of transporters for efficient carbon and nitrogen acquisition from the nutritionally rich environments they inhabit and reflect a limited range of biosynthetic capabilities that indicate both prototrophic and auxotrophic strains. Phylogenetic analyses, comparison of gene content across the group, and reconstruction of ancestral gene sets indicate a combination of extensive gene loss and key gene acquisitions via horizontal gene transfer during the coevolution of lactic acid bacteria with their habitats.

  5. Methods for dispersing hydrocarbons using autoclaved bacteria

    DOE Patents [OSTI]

    Tyndall, R.L.

    1996-11-26T23:59:59.000Z

    A method of dispersing a hydrocarbon includes the following steps: providing a bacterium selected from the following group: ATCC 85527, ATCC 75529, and ATCC 55638, a mutant of any one of these bacteria possessing all the identifying characteristics of any one of these bacteria, and mixtures; autoclaving the bacterium to derive a dispersant solution; and contacting the dispersant solution with a hydrocarbon to disperse the hydrocarbon. Moreover, a method for preparing a dispersant solution includes the following steps: providing a bacterium selected from the following group: ATCC 75527, ATCC 75529, and ATCC 55638, a mutant of any one of these bacteria possessing all the identifying characteristics of any one of these bacteria, and mixtures; and autoclaving the bacterium to derive a dispersant solution.

  6. Probiotic Bacteria Induce a ‘Glow of Health’

    E-Print Network [OSTI]

    Levkovich, Tatiana

    Radiant skin and hair are universally recognized as indications of good health. However, this ‘glow of health’ display remains poorly understood. We found that feeding of probiotic bacteria to aged mice induced integumentary ...

  7. A BUCKEYE'S GUIDE TO LIVING OFF-CAMPUS LIVING GUIDE

    E-Print Network [OSTI]

    Howat, Ian M.

    2014­2015 FREE A BUCKEYE'S GUIDE TO LIVING OFF-CAMPUS LIVING GUIDE OFFICE OF STUDENT LIFE@osu.edu offcampus.osu.edu WELCOME TO OFF-CAMPUS LIVING! 3 #12;TABLE OF CONTENTS OFFICE OF STUDENT LIFE 9 62 Stay Healthy 66 The Basics 67 Get Involved 68 Moving Out 5 #12;1 Bedrooms: 2262 N. H IG H 49 E. N

  8. Phase Preference by Active, Acetate-Utilizing Bacteria at the Rifle, CO Integrated Field Research Challenge Site

    SciTech Connect (OSTI)

    Kerkhoff, Lee; Williams, Kenneth H.; Long, Philip E.; McGuinness, L.

    2011-02-15T23:59:59.000Z

    Uranium contaminated groundwaters are a legacy concern for the U.S. Department of Energy. Previous experiments at the Rifle, Colorado Integrated Field Challenge (IFC) site have demonstrated that field-scale addition of acetate to groundwater reduces the ambient soluable uranium concentration, sequestering the radionuclide as uraninite. However, questions remain regarding which microorganism(s) are consuming this acetate and if active groundwater microorganisms are different from active particle-associated bacteria. In this report, 13-C acetate was used to assess the active microbes that synthesize DNA on 3 size fractions [coarse sand, fines (8-approximately 150 micron), groundwater (0.2-8 micron)] over a 24 -day time frame. Results indicated a stronger signal from 13-C acetate associated with the “fines” fraction compared with smaller amounts of 13-C uptake on the sand fraction and groundwater samples during the SIP incubations. TRFLP analysis of this 13-C-labeled DNA, indicated 31+ 9 OTU's with 6 peaks dominating the active profiles (166, 187, 210, 212, and 277 bp peaks using MnlI). Cloning/sequencing of the amplification products indicated a Geobacter-like group (187, 210, 212 bp) primarily synthesized DNA from acetate in the groundwater phase, an alpha Proteobacterium (166 bp) primarily grew on the fines/sands, and an Acinetobacter sp. (277 bp) utilized much of the 13C acetate in both groundwater and particle-associated phases. These findings will help to delineate the acetate utilization patterns of bacteria during field-scale acetate addition and can lead to improved methods for stimulating distinct microbial populations in situ.

  9. Agencies Approve Bacteria TMDL Task Force Recommendations 

    E-Print Network [OSTI]

    Wythe, Kathy

    2007-01-01T23:59:59.000Z

    tx H2O | pg. 10 In June 2007 the Texas Commission onEnvironmental Quality (TCEQ) and the TexasState Soil and Water Conservation Board (TSSW- CB) approved the recommendations of the Bacteria Total Maximum Daily Load (TMDL) Task Force and asked... their agencies to update their TMDL guidance documents to reflect these recommendations. They also authorized establishing a multi-agency bacteria TMDL work group to examine the research and development needs identified in the task force report. Both TCEQ...

  10. Chemotactic selection of pollutant degrading soil bacteria

    DOE Patents [OSTI]

    Hazen, Terry C. (Augusta, GA)

    1994-01-01T23:59:59.000Z

    A method for identifying soil microbial strains which may be bacterial degraders of pollutants comprising the steps of placing a concentration of a pollutant in a substantially closed container, placing the container in a sample of soil for a period of time ranging from one minute to several hours, retrieving the container, collecting the contents of the container, and microscopically determining the identity of the bacteria present. Different concentrations of the pollutant can be used to determine which bacteria respond to each concentration. The method can be used for characterizing a polluted site or for looking for naturally occurring biological degraders of the pollutant. Then bacteria identified as degraders of the pollutant and as chemotactically attracted to the pollutant are used to inoculate contaminated soil. To enhance the effect of the bacteria on the pollutant, nutrients are cyclicly provided to the bacteria then withheld to alternately build up the size of the bacterial colony or community and then allow it to degrade the pollutant.

  11. Meso-scale turbulence in living fluids

    E-Print Network [OSTI]

    Henricus H. Wensink; Jörn Dunkel; Sebastian Heidenreich; Knut Drescher; Raymond E. Goldstein; Hartmut Löwen; Julia M. Yeomans

    2012-08-21T23:59:59.000Z

    Turbulence is ubiquitous, from oceanic currents to small-scale biological and quantum systems. Self-sustained turbulent motion in microbial suspensions presents an intriguing example of collective dynamical behavior amongst the simplest forms of life, and is important for fluid mixing and molecular transport on the microscale. The mathematical characterization of turbulence phenomena in active non-equilibrium fluids proves even more difficult than for conventional liquids or gases. It is not known which features of turbulent phases in living matter are universal or system-specific, or which generalizations of the Navier-Stokes equations are able to describe them adequately. Here, we combine experiments, particle simulations, and continuum theory to identify the statistical properties of self-sustained meso-scale turbulence in active systems. To study how dimensionality and boundary conditions affect collective bacterial dynamics, we measured energy spectra and structure functions in dense Bacillus subtilis suspensions in quasi-2D and 3D geometries. Our experimental results for the bacterial flow statistics agree well with predictions from a minimal model for self-propelled rods, suggesting that at high concentrations the collective motion of the bacteria is dominated by short-range interactions. To provide a basis for future theoretical studies, we propose a minimal continuum model for incompressible bacterial flow. A detailed numerical analysis of the 2D case shows that this theory can reproduce many of the experimentally observed features of self-sustained active turbulence.

  12. The living publication

    SciTech Connect (OSTI)

    Terwilliger, Thomas C. [Los Alamos National Laboratory

    2012-06-04T23:59:59.000Z

    Within the ICSTI Insights Series we offer three articles on the 'living publication' that is already available to practitioners in the important field of crystal structure determination and analysis. While the specific examples are drawn from this particular field, we invite readers to draw parallels in their own fields of interest. The first article describes the present state of the crystallographic living publication, already recognized by an ALPSP (Association of Learned and Professional Society Publishers) Award for Publishing Innovation in 2006. The second article describes the potential impact on the record of science as greater post-publication analysis becomes more common within currently accepted data deposition practices, using processed diffraction data as the starting point. The third article outlines a vision for the further improvement of crystallographic structure reports within potentially achievable enhanced data deposition practices, based upon raw (unprocessed) diffraction data. The IUCr in its Commissions and Journals has for many years emphasized the importance of publications being accompanied by data and the interpretation of the data in terms of atomic models. This has been followed as policy by numerous other journals in the field and its cognate disciplines. This practice has been well served by databases and archiving institutions such as the Protein Data Bank (PDB), the Cambridge Crystallographic Data Centre (CCDC), and the Inorganic Crystal Structure Database (ICSD). Normally the models that are archived are interpretations of the data, consisting of atomic coordinates with their displacement parameters, along with processed diffraction data from X-ray, neutron or electron diffraction studies. In our current online age, a reader can not only consult the printed word, but can display and explore the results with molecular graphics software of exceptional quality. Furthermore, the routine availability of processed diffraction data allows readers to perform direct calculations of the electron density (using X-rays and electrons as probes) or the nuclear density (using neutrons as probe) on which the molecular models are directly based. This current community practice is described in our first article. There are various ways that these data and tools can be used to further analyze the molecules that have been crystallized. Notably, once a set of results is announced via the publication, the research community can start to interact directly with the data and models. This gives the community the opportunity not only to read about the structure, but to examine it in detail, and even generate subsequent improved models. These improved models could, in principle, be archived along with the original interpretation of the data and can represent a continuously improving set of interpretations of a set of diffraction data. The models could improve both by correction of errors in the original interpretation and by the use of new representations of molecules in crystal structures that more accurately represent the contents of a crystal. These possible developments are described in our second article. A current, significant, thrust for the IUCr is whether it would be advantageous for the crystallographic community to require, rather than only encourage, the archiving of the raw (unprocessed) diffraction data images measured from a crystal, a fibre or a solution. This issue is being evaluated in detail by an IUCr Working Group (see http://forums.iucr.org). Such archived raw data would be linked to and from any associated publications. The archiving of raw diffraction data could allow as yet undeveloped processing methods to have access to the originally measured data. The debate within the community about this much larger proposed archiving effort revolves around the issue of 'cost versus benefit'. Costs can be minimized by preserving the raw data in local repositories, either at centralized synchrotron and neutron research institutes, or at research universities. Archiving raw data is also perceived as bein

  13. Biofuel from Bacteria and Sunlight: Shewanella as an Ideal Platform for Producing Hydrocarbons

    SciTech Connect (OSTI)

    None

    2010-01-01T23:59:59.000Z

    Broad Funding Opportunity Announcement Project: The University of Minnesota is developing clean-burning, liquid hydrocarbon fuels from bacteria. The University is finding ways to continuously harvest hydrocarbons from a type of bacteria called Shewanella by using a photosynthetic organism to constantly feed Shewanella the sugar it needs for energy and hydrocarbon production. The two organisms live and work together as a system. Using Shewanella to produce hydrocarbon fuels offers several advantages over traditional biofuel production methods. First, it eliminates many of the time-consuming and costly steps involved in growing plants and harvesting biomass. Second, hydrocarbon biofuels resemble current petroleum-based fuels and would therefore require few changes to the existing fuel refining and distribution infrastructure in the U.S.

  14. What is compost? Composting refers to biological decomposition and stabilization of organic materials by microorganisms under

    E-Print Network [OSTI]

    Mukhtar, Saqib

    What is compost? Composting refers to biological decomposition and stabilization of organic materials by microorganisms under aerobic conditions (in the presence of oxygen). During the composting is production of good-quality compost that is biologically stable, relatively uniform in appearance, free

  15. The Living Culture of Nigeria

    E-Print Network [OSTI]

    Biobaku, Saburi O.; Aniakor, Chike A.

    1977-01-01T23:59:59.000Z

    ImK ~IE.W THE uv:rn:; aJLWRE OF NIGERIA edited by Saburi 0 .Lagos: Thanas Nelson (Nigeria) Ltd. , Color Illustrations,86 The Living Cu'lture of Nigeria edited by Professor Saburi

  16. Blood Banking in Living Droplets

    E-Print Network [OSTI]

    Samot, Josh

    Blood banking has a broad public health impact influencing millions of lives daily. It could potentially benefit from emerging biopreservation technologies. However, although vitrification has shown advantages over traditional ...

  17. Sampling for Bacteria in Wells (Spanish) 

    E-Print Network [OSTI]

    Lesikar, Bruce J.

    2001-11-15T23:59:59.000Z

    general es de entre $8.00 y $25.00. La bacteria de hierro es otro contaminante que forma lama muy obvia dentro de los tubos y los grifos. No se necesita un an?lisis de laboratorio para identificarla. Busque una lama color caf? o rojiza dentro del tanque...

  18. Comparative study of four fluorinated quinolones in susceptibility factors on microorganisms 

    E-Print Network [OSTI]

    Patel, Ketul R

    2013-02-22T23:59:59.000Z

    . This study hopes to prove an equal effectiveness of enrofloxacin and ciprofloxacin. Microbial susceptibility to drugs is measured by determining the MIC (Minimum Inhibitory Concentration). The MIC is measured based on the growth of the targeted bacteria...

  19. Human Occupancy as a Source of Indoor Airborne Bacteria

    E-Print Network [OSTI]

    Hospodsky, Denina

    Exposure to specific airborne bacteria indoors is linked to infectious and noninfectious adverse health outcomes. However, the sources and origins of bacteria suspended in indoor air are not well understood. This study ...

  20. Hypermutation and adaptation of experimentally evolved marine Vibrio bacteria

    E-Print Network [OSTI]

    Clarke, Sean Aidan

    2013-01-01T23:59:59.000Z

    Environmental bacteria display tremendous genetic diversity, but we are still learning how this diversity arises and relates to their wide range of habitats. Investigating how bacteria adapt helps us understand their ...

  1. High-throughput experimental and computational tools for exploring immunity and the microbiome

    E-Print Network [OSTI]

    Papa, Eliseo

    2012-01-01T23:59:59.000Z

    Humans live in association with trillions of microbes and yet we know remarkably little about their symbiotic relationship. The role these microorganisms have in humans has been characterized only in the case of few bacteria ...

  2. Natural Oil Production from Microorganisms: Bioprocess and Microbe Engineering for Total Carbon Utilization in Biofuel Production

    SciTech Connect (OSTI)

    None

    2010-07-15T23:59:59.000Z

    Electrofuels Project: MIT is using carbon dioxide (CO2) and hydrogen generated from electricity to produce natural oils that can be upgraded to hydrocarbon fuels. MIT has designed a 2-stage biofuel production system. In the first stage, hydrogen and CO2 are fed to a microorganism capable of converting these feedstocks to a 2-carbon compound called acetate. In the second stage, acetate is delivered to a different microorganism that can use the acetate to grow and produce oil. The oil can be removed from the reactor tank and chemically converted to various hydrocarbons. The electricity for the process could be supplied from novel means currently in development, or more proven methods such as the combustion of municipal waste, which would also generate the required CO2 and enhance the overall efficiency of MIT’s biofuel-production system.

  3. The Characterization of Psychrophilic Microorganisms and their potentially useful Cold-Active Glycosidases Final Progress Report

    SciTech Connect (OSTI)

    Brenchly, Jean E.

    2008-06-30T23:59:59.000Z

    Our studies of novel, cold-loving microorganisms have focused on two distinct extreme environments. The first is an ice core sample from a 120,000 year old Greenland glacier. The results of this study are particularly exciting and have been highlighted with press releases and additional coverage. The first press release in 2004 was based on our presentation at the General Meeting of the American Society for Microbiology and was augmented by coverage of our publication (Appl. Environ. Microbiol. 2005. Vol. 71:7806) in the Current Topics section of the ASM news journal, “Microbe.” Of special interest for this report was the isolation of numerous, phylogenetically distinct and potentially novel ultrasmall microorganisms. The detection and isolation of members of the ultrasmall population is significant because these cells pass through 0.2 micron pore filters that are generally used to trap microorganisms from environmental samples. Thus, analyses by other investigators that examined only cells captured on the filters would have missed a significant portion of this population. Only a few ultrasmall isolates had been obtained prior to our examination of the ice core samples. Our development of a filtration enrichment and subsequent cultivation of these organisms has added extensively to the collection of, and knowledge about, this important population in the microbial world.

  4. (Catalytic mechanism of hydrogenase from aerobic N sub 2 -fixing microorganisms)

    SciTech Connect (OSTI)

    Arp, D.J.

    1990-01-01T23:59:59.000Z

    Hydrogenases are enzymes which catalyze reactions involving dihydrogen. They serve integral roles in a number of microbial metabolic pathways. Our research is focussed on investigations of the catalytic mechanism of the hydrogenases found in aerobic, N{sub 2}-fixing microorganisms such as Azotobacter vinelandii and the agronomically important Bradyrhizobium japonicum as well as microorganisms with similar hydrogenases. The hydrogenases isolated from these microorganisms are Ni- and Fe-containing heterodimers. Our work has focussed on three areas during the last grant period. In all cases, a central theme has been the role of inhibitors in the characteristics under investigation. In addition, a number of collaborative efforts have yielded interesting results. In metalloenzymes such as hydrogenase, inhibitors often influence the activity of the enzyme through ligand interactions with redox centers, often metals, within the enzyme. Therefore, investigations of the ability of various compounds to inhibit an enzyme's activity, as well as the mechanism of inhibition, can provide insight into the catalytic mechanism of the enzyme as well as the role of various redox centers in catalysis. We have investigated in detail four inhibitors of A. vinelandii and the results are summarized here. The influence of these inhibitors on the spectral properties of the enzyme are summarized. Electron paramagnetic resonance and ultraviolet spectra investigations are discussed. 9 figs.

  5. Subsurface interactions of actinide species and microorganisms : implications for the bioremediation of actinide-organic mixtures.

    SciTech Connect (OSTI)

    Banaszak, J.E.; Reed, D.T.; Rittmann, B.E.

    1999-02-12T23:59:59.000Z

    By reviewing how microorganisms interact with actinides in subsurface environments, we assess how bioremediation controls the fate of actinides. Actinides often are co-contaminants with strong organic chelators, chlorinated solvents, and fuel hydrocarbons. Bioremediation can immobilize the actinides, biodegrade the co-contaminants, or both. Actinides at the IV oxidation state are the least soluble, and microorganisms accelerate precipitation by altering the actinide's oxidation state or its speciation. We describe how microorganisms directly oxidize or reduce actinides and how microbiological reactions that biodegrade strong organic chelators, alter the pH, and consume or produce precipitating anions strongly affect actinide speciation and, therefore, mobility. We explain why inhibition caused by chemical or radiolytic toxicities uniquely affects microbial reactions. Due to the complex interactions of the microbiological and chemical phenomena, mathematical modeling is an essential tool for research on and application of bioremediation involving co-contamination with actinides. We describe the development of mathematical models that link microbiological and geochemical reactions. Throughout, we identify the key research needs.

  6. Biochemistry and physiology of anaerobic bacteria

    SciTech Connect (OSTI)

    NONE

    2000-05-18T23:59:59.000Z

    We welcome you to The Power of Anaerobes. This conference serves two purposes. One is to celebrate the life of Harry D. Peck, Jr.,who was born May 18, 1927 and would have celebrated his 73rd birthday at this conference. He died November 20, 1998. The second is to gather investigators to exchange views within the realm of anaerobic microbiology, an area in which tremendous progress has been seen during recent years. It is sufficient to mention discoveries of a new form of life (the archaea), hyper or extreme thermophiles, thermophilic alkaliphiles and anaerobic fungi. With these discoveries has come a new realization about physiological and metabolic properties of microorganisms, and this in turn has demonstrated their importance for the development, maintenance and sustenance of life on Earth.

  7. Attachment and detachment of microorganisms as related to sampling carcasses and meat products 

    E-Print Network [OSTI]

    Butler, Jacqueline Love

    1980-01-01T23:59:59.000Z

    of Advisory Committee: Dr. C. Vanderzant A model system was developed to study attachment and de- t-chment of' bacteria with pork skin and muscle surfaces of beef and lamb carcasses. The technique involved embedding pork skin and beef and lamb muscle... medium, although in some cases attachment continued to occur over a $0-min period. Gram-negative motile bacteria had greater force of attachment than gram-positive non-motile species. Temperature and pH of the attachment medium had little eff...

  8. A comparison of taxon co-occurrence patterns for macro- and microorganisms

    E-Print Network [OSTI]

    Horner-Devine, M. Claire; Silver, Jessica M.; Leibold, Mathew A.; Bohannan, Brendan J. M.; Colwell, Robert K.; Fuhrman, Jed A.; Green, Jessica L.; Kuske, Cheryl R.; Martiny, Jennifer B. H.; Muyzer, Gerard; Ø vreå s, Lise; Reysenbach, Anna-Louise; Smith, Val H.

    2007-06-01T23:59:59.000Z

    . Holmes, M. Westoby, I. Oliver, D. Briscoe, M. Dangerfield, M. Gillings, and A. Beattie. 2004. Spatial scaling of microbial eukaryote diversity. Nature 432:747–750. Hillebrand, H., F. Watermann, R. Karez, and U. G. Berninger. 2001. Differences in species...Ecology, 88(6), 2007, pp. 1345–1353 #1; 2007 by the Ecological Society of America A COMPARISON OF TAXON CO-OCCURRENCE PATTERNS FOR MACRO- AND MICROORGANISMS M. CLAIRE HORNER-DEVINE,1,13 JESSICA M. SILVER,1 MATHEW A. LEIBOLD,2 BRENDAN J. M. BOHANNAN...

  9. International symposium on cellular and molecular biology of phosphate and phosphorylated compounds in microorganisms: Proceedings

    SciTech Connect (OSTI)

    NONE

    1993-12-31T23:59:59.000Z

    This report contains the abstracts of papers presented at the conference. Attention is focused on the following topics: regulation of phosphate metabolism in bacteria; structure-function of alkaline phosphatase; regulation of phosphate metabolism in yeast; transport of phosphate and phosphorylated compounds; and phosphate regulation in pathogenesis and secondary metabolism.

  10. Typical College Student Diet Food Derived Microorganisms and Their Relation to the Human Gastrointestinal Microflora 

    E-Print Network [OSTI]

    Haselhorst, Alexandria J.

    2011-08-08T23:59:59.000Z

    or not different foods contain unique bacterial profiles and if there is a correlation between foods and the types of bacteria that may be present. This study was based on diets that are typically consumed by college students. Six meals, two breakfasts, two lunches...

  11. Towards Plasma Surgery: Plasma Treatment of Living Cells

    SciTech Connect (OSTI)

    Stoffels, E.; Kieft, I.E.; Sladek, R.E.J.; Slaaf, D.W.; Laan, E.P. van der; Jimenez-Moreno, P.; Steinbuch, M. [Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB, Eindhoven (Netherlands); Department of Mechanical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB, Eindhoven (Netherlands)

    2004-12-01T23:59:59.000Z

    The physical, biological and technical background for high-precision plasma surgery is prepared in a multi-disciplinary team. The aim of the research is to achieve controlled removal of diseased cells and bacteria without harming the healthy rest of the tissue. For this purpose, a small, cold, flexible and non-toxic plasma is developed (the plasma needle) and tested on cultured cells and bacterial samples. The needle is an atmospheric discharge induced by a radio-frequency voltage applied to a metal pin. This plasma operates at room temperature, in the milliwatt power regime; it poses no risk of thermal or electrical damage to living tissues. Several beneficial responses of living cells to plasma treatment have been already identified. Plasma does not cause accidental cell death (necrosis), which leads to inflammation and tissue damage. Instead, it allows to detach cells from each other and from the scaffold, and thus to remove them in a non-destructive way. Moreover, plasma is capable of bacterial inactivation. I parallel, we have determined the electrical and optical properties of the plasma and found a method of precise positioning of the plasma needle with respect to the treated tissue.

  12. Detection of phenols using engineered bacteria

    DOE Patents [OSTI]

    Wise, Arlene A. (Philadelphia, PA); Kuske, Cheryl R. (Los Alamos, NM); Terwilliger, Thomas C. (Santa Fe, NM)

    2007-12-04T23:59:59.000Z

    Detection of phenols using engineered bacteria. A biosensor can be created by placing a reporter gene under control of an inducible promoter. The reporter gene produces a signal when a cognate transcriptional activator senses the inducing chemical. Creation of bacterial biosensors is currently restricted by limited knowledge of the genetic systems of bacteria that catabolize xenobiotics. By using mutagenic PCR to change the chemical specificity of the Pseudomonas species CF600 DmpR protein, the potential for engineering novel biosensors for detection of phenols has been demonstrated. DmpR, a well-characterized transcriptional activator of the P. CF600's dmp operon mediates growth on simple phenols. Transcription from Po, the promoter heading the dmp operon, is activated when the sensor domain of DmpR interacts with phenol and mono-substituted phenols. By altering the sensor domain of the DmpR, a group of DmpR derivatives that activate transcription of a Po-lacZ fusion in response to eight of the EPA's eleven priority pollutant phenols has been created. The assays and the sensor domain mutations that alter the chemical specificity of DmpR is described.

  13. Detection of phenols using engineered bacteria

    DOE Patents [OSTI]

    Wise, Arlene A.; Kuske, Cheryl R.; Terwilliger, Thomas C.

    2004-08-10T23:59:59.000Z

    Detection of phenols using engineered bacteria. A biosensor can be created by placing a reporter gene under control of an inducible promoter. The reporter gene produces a signal when a cognate transcriptional activator senses the inducing chemical. Creation of bacterial biosensors is currently restricted by limited knowledge of the genetic systems of bacteria that catabolize xenobiotics. By using mutagenic PCR to change the chemical specificity of the Pseudomonas species CF600 DmpR protein, the potential for engineering novel biosensors for detection of phenols has been demonstrated. DmpR, a well-characterized transcriptional activator of the P. CF600's dmp operon mediates growth on simple phenols. Transcription from Po, the promoter heading the dmp operon, is activated when the sensor domain of DmpR interacts with phenol and mono-substituted phenols. By altering the sensor domain of the DmpR, a group of DmpR derivatives that activate transcription of a Po-lacZ fusion in response to eight of the EPA's eleven priority pollutant phenols has been created. The assays and the sensor domain mutations that alter the chemical specificity of DmpR is described.

  14. Leachability of salmonella and fecal pollution indicator bacteria through soil

    E-Print Network [OSTI]

    Fehrmann, Robert Clinton

    1977-01-01T23:59:59.000Z

    Factors controlling bs. cterial movement Movement of Salmonella in soils MATERIALS AND METHODS Soil ana1ysis Inoculum preparation Soil column preparation Bacterial adsorption to soil particles Ei'fects of different salts on the leaching of bacteria... Distribution of bacteria in soil columns Bacterial saturation of soils Break through curves Enumeration of bacteria Bacterial size 1. 4 17 17 18 18 1 9 19 20 Field studies Statistics RESULTS AND DISCUSSION 20 21 22 Filter selection...

  15. acid bacteria revisited: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Summary: The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Clark of amino acid in soil using radioactive isotopes, however many experiments...

  16. acid bacteria elicit: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Summary: The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Clark of amino acid in soil using radioactive isotopes, however many experiments...

  17. acid bacteria affect: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Summary: The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Clark of amino acid in soil using radioactive isotopes, however many experiments...

  18. acid bacteria structure: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Summary: The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Clark of amino acid in soil using radioactive isotopes, however many experiments...

  19. acid bacteria inducing: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Summary: The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Clark of amino acid in soil using radioactive isotopes, however many experiments...

  20. acid bacteria purification: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Summary: The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Clark of amino acid in soil using radioactive isotopes, however many experiments...

  1. acid bacteria enhance: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    de 4 The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Environmental Sciences and Ecology Websites Summary: The Fate of Amino Acid in...

  2. Transfer of noncoding DNA drives regulatory rewiring in bacteria

    E-Print Network [OSTI]

    Oren, Yaara

    Understanding the mechanisms that generate variation is a common pursuit unifying the life sciences. Bacteria represent an especially striking puzzle, because closely related strains possess radically different metabolic ...

  3. Cytotoxic Cells Kill Intracellular Bacteria through Granulysin-Mediated

    E-Print Network [OSTI]

    Kirchhausen, Tomas

    , cytotoxic granules also contain granulysin, an antimicrobial peptide. Here, we show that granulysin delivers (ROS) that rapidly kill bacteria. ROS scavengers and bac- terial antioxidant protein overexpression

  4. acid bacteria reveals: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Diversity and characterization of sulfate-reducing bacteria in groundwater at a uranium mill tailings site CiteSeer Summary: plays a role in both natural attenuation and...

  5. anaerobic phototrophic bacteria: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    bacteria convert organic matter into methane and carbon dioxide (a mixture called biogas) in the absence of air. It is a widely used biological process for treating wastewater...

  6. Solo living across the adult lifecourse 

    E-Print Network [OSTI]

    Smith, Adam; Wasoff, Fran; Jamieson, Lynn

    This study has created snapshots of solo living in contemporary society and developed an understanding of the social and economic factors involved in transitions in and out of solo living.

  7. Detection and quantification of waterborne microorganisms using an image cytometer based on angular spatial frequency processing

    E-Print Network [OSTI]

    Pérez, Juan Miguel; Martínez, Pedro; Pruneri, Valerio

    2015-01-01T23:59:59.000Z

    We introduce a new image cytometer design for detection of very small particulate and demonstrate its capability in water analysis. The device is a compact microscope composed of off--the--shelf components, such as a light emitting diode (LED) source, a complementary metal--oxide--semiconductor (CMOS) image sensor, and a specific combination of optical lenses that allow, through an appropriate software, Fourier transform processing of the sample volume. Waterborne microorganisms, such as Escherichia coli (E. coli), Legionella pneumophila (L. pneumophila) and Phytoplankton, are detected by interrogating the volume sample either in a fluorescent or label-free mode, i.e. with or without fluorescein isothiocyanate (FITC) molecules attached to the micro-organisms, respectively. We achieve a sensitivity of 50 CFU/ml, which can be further increased to 0.2 CFU/ml by pre-concentrating an initial sample volume of 500 ml with an ad hoc fluidic system. We also prove the capability of the proposed image cytometer of diffe...

  8. Living in Germany. Studying at FAU.

    E-Print Network [OSTI]

    Fiebig, Peter

    Welcome. Living in Germany. Studying at FAU. www.fau.eu/international #12;Welcome. Living in Germany. Studying at FAU. 2 Welcome. Living in Germany. Studying at FAU. Contents Vice President`s welcome...................................................... 5 1. What you will find in this brochure .............................. 6 2. Welcome to Germany

  9. Live Streaming with Gossip Maxime Monod

    E-Print Network [OSTI]

    Guerraoui, Rachid

    Live Streaming with Gossip Maxime Monod June 30, 2010 #12;Regular TV: everything HD Live streaming A source produces multimedia content n viewers (n large) broadcasting ... ... ... IP TV, Web TV, P2P TV environment ·HEAP Heterogeneous environment ·LiFT Presence of freeriders Live Streaming with Gossip 25 #12

  10. Energy conversion in Purple Bacteria Photosynthesis

    E-Print Network [OSTI]

    Felipe Caycedo-Soler; Ferney J. Rodriguez; Luis Quiroga; Guannan Zhao; Neil F. Johnson

    2011-07-01T23:59:59.000Z

    The study of how photosynthetic organisms convert light offers insight not only into nature's evolutionary process, but may also give clues as to how best to design and manipulate artificial photosynthetic systems -- and also how far we can drive natural photosynthetic systems beyond normal operating conditions, so that they can harvest energy for us under otherwise extreme conditions. In addition to its interest from a basic scientific perspective, therefore, the goal to develop a deep quantitative understanding of photosynthesis offers the potential payoff of enhancing our current arsenal of alternative energy sources for the future. In the following Chapter, we consider the trade-off between dynamics, structure and function of light harvesting membranes in Rps. Photometricum purple bacteria, as a model to highlight the priorities that arise when photosynthetic organisms adapt to deal with the ever-changing natural environment conditions.

  11. Energy conversion in Purple Bacteria Photosynthesis

    E-Print Network [OSTI]

    Caycedo-Soler, Felipe; Quiroga, Luis; Zhao, Guannan; Johnson, Neil F

    2011-01-01T23:59:59.000Z

    The study of how photosynthetic organisms convert light offers insight not only into nature's evolutionary process, but may also give clues as to how best to design and manipulate artificial photosynthetic systems -- and also how far we can drive natural photosynthetic systems beyond normal operating conditions, so that they can harvest energy for us under otherwise extreme conditions. In addition to its interest from a basic scientific perspective, therefore, the goal to develop a deep quantitative understanding of photosynthesis offers the potential payoff of enhancing our current arsenal of alternative energy sources for the future. In the following Chapter, we consider the trade-off between dynamics, structure and function of light harvesting membranes in Rps. Photometricum purple bacteria, as a model to highlight the priorities that arise when photosynthetic organisms adapt to deal with the ever-changing natural environment conditions.

  12. SF State | Residential Life Community Living Standards Page 1 COMMUNITY LIVING STANDARDS

    E-Print Network [OSTI]

    SF State | Residential Life Community Living Standards Page 1 COMMUNITY LIVING STANDARDS A How-To Guide to Living in the SF State Residential Community 2014-2015 #12;SF State | Residential Life Community Philosophy Residential Life Staff Campus Resources One Stop Your Roommate Your Living Space

  13. The role of microorganisms in the production of volatile sulfhydryl compounds in cheddar cheese slurries

    E-Print Network [OSTI]

    Ponce-Trevino, Raul

    1988-01-01T23:59:59.000Z

    the role of lac- tic cultures on the production of volatile sulfhydryl com- pounds in the slurries. Hydrogen sulfide, methanethiol, carbonyl sulfide and dimethyl sulfide were found in the headspace of slurries prepared from curd manufactured... by the culture procedure. Addition of an antibiotic mixture to slurries prepared from curd manufactured by the culture me- thod inhibited bacteria growth and the production of hydro- gen sulfide and dimethyl sulfide. A mixture of antibiotics was added...

  14. Typical College Student Diet Food Derived Microorganisms and Their Relation to the Human Gastrointestinal Microflora

    E-Print Network [OSTI]

    Haselhorst, Alexandria J.

    2011-08-08T23:59:59.000Z

    not yet concrete (11). These probiotics are presently sold commercially in fermented dairy products such as yogurt. Dannon?s Activia? (which contain Bifidus regularis?) and Yakult? (which contains Lactobacillus casei) are examples of such product.... There are reports that in the future, these probiotics may be found in fermented vegetables and meat (11). Prebiotics can be defined as non-digestible food that beneficially affects the host by selectively stimulating the growth and/or activity of bacteria...

  15. Magnetic minerals produced by magnetotactic bacteria Balzs Arat1

    E-Print Network [OSTI]

    Dunin-Borkowski, Rafal E.

    Magnetic minerals produced by magnetotactic bacteria Balázs Arató1 , Mihály Pósfai1 and Rafal E-controlled mineralization Abstract. Magnetotactic bacteria produce intracellular magnetic minerals that have distinct for studying the biological membrane around the mineral grains. Our goals were to deduce the possible growth

  16. Characteristics of cyclic AMP transport by marine bacteria

    SciTech Connect (OSTI)

    Ammerman, J.W.; Azam, F.

    1987-12-01T23:59:59.000Z

    Uptake and autoradiography experiments with natural populations of marine bacteria, sea water cultures, and cultured isolates showed that the high-affinity cyclic AMP transport system in marine bacteria has stringent structural requirements, is found in a minority of cells in mixed bacterial assemblages, and appears to be related to the culture growth state.

  17. adolescent population living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    that adolescents assign (more) Amos, Taryn 2009-01-01 94 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  18. actual living liver: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    - Liver EQA slides' 2. Electronic submissions Magee, Derek 67 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  19. active living logan: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Kenong Wu; David Gauthier; Martin D. Levine 1993-01-01 126 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  20. abortus live vaccine: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    healthcare needs. TheVirginia Tech Virginia Tech 183 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  1. active living principles: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    theory of generalized functions. Vernaeve, Hans 2011-01-01 235 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  2. african americans living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of human African trypanosomiasis (HAT Schnaufer, Achim 375 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  3. american indians living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    positive correlations with the Indian Chang, Chih-Pei 262 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  4. advanced cancer living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    direct and (more) Duberow, Jr, David Paul 2009-01-01 100 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  5. attenuated live vaccine: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    healthcare needs. TheVirginia Tech Virginia Tech 211 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  6. adult living donor: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    to extending our successes in childhood unknown authors 116 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  7. american women living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    introduces teachers to Maya culture from Saldin, Dilano 295 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  8. admixed population lived: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    population in urban United Nations; Nations Unies 1999-01-01 67 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  9. acomys cahirinus living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Kenong Wu; David Gauthier; Martin D. Levine 1993-01-01 18 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  10. active living projects: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Brian Meyers; John Krumm; A Kern; Steven Shafer 2000-01-01 236 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  11. attenuated live infectious: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    R. Matyas; Victoria R. Polonis; Mangala Rao; Carl R. Alving 169 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  12. analysis reveals living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Oleg V. Gradov; Margaret A. Gradova 2015-01-18 184 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  13. arab americans living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Syria. The three countries show different Shavitt, Yuval 380 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  14. adolescent girls living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    that adolescents assign (more) Amos, Taryn 2009-01-01 139 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  15. african women living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Siegal 1991). Marvin P. Dawkins; Mary M. Williams 273 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  16. active living environment: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and physical activity environment audit tools Keinan, Alon 146 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  17. asthmatic children living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Texas; Kevin Purcell; Jaime Fergie; Kevin Richman; Lisa Rocha 84 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  18. active living study: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Epstein, and Li Deng) and a computational Snider, Barry B. 215 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  19. Aerobic mineralization of MTBE and tert-butyl alcohol by stream-bed sediment microorganisms

    SciTech Connect (OSTI)

    Bradley, P.M.; Landmeyer, J.E.; Chapelle, F.H. [Geological Survey, Columbia, SC (United States)] [Geological Survey, Columbia, SC (United States)

    1999-06-01T23:59:59.000Z

    Microorganisms indigenous to the stream-bed sediments at two gasoline-contaminated groundwater sites demonstrated significant mineralization of the fuel oxygenates, methyl tert-butyl ether (MTBE) and tert-butyl alcohol (TBA). Up to 73% of [U-{sup 14}C]-MTBE and 84% of [U-{sup 14}C]-TBA were degraded to {sup 14}CO{sub 2} under mixed aerobic/anaerobic conditions. No significant mineralization was observed under strictly anaerobic conditions. The results indicate that, under the mixed aerobic/anaerobic conditions characteristic of stream-bed sediments, microbial processes may provide a significant environmental sink for MTBE and TBA delivered to surface water bodies by contaminated groundwater or by other sources.

  20. Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels

    SciTech Connect (OSTI)

    Kuk Lee, Sung; Chou, Howard; Ham, Timothy S.; Soon Lee, Taek; Keasling, Jay D.

    2009-12-02T23:59:59.000Z

    The ability to generate microorganisms that can produce biofuels similar to petroleum-based transportation fuels would allow the use of existing engines and infrastructure and would save an enormous amount of capital required for replacing the current infrastructure to accommodate biofuels that have properties significantly different from petroleum-based fuels. Several groups have demonstrated the feasibility of manipulating microbes to produce molecules similar to petroleum-derived products, albeit at relatively low productivity (e.g. maximum butanol production is around 20 g/L). For cost-effective production of biofuels, the fuel-producing hosts and pathways must be engineered and optimized. Advances in metabolic engineering and synthetic biology will provide new tools for metabolic engineers to better understand how to rewire the cell in order to create the desired phenotypes for the production of economically viable biofuels.

  1. How sulphate-reducing microorganisms cope with stress: Lessons from systems biology

    SciTech Connect (OSTI)

    Zhou, J.; He, Q.; Hemme, C.L.; Mukhopadhyay, A.; Hillesland, K.; Zhou, A.; He, Z.; Nostrand, J.D. Van; Hazen, T.C.; Stahl, D.A.; Wall, J.D.; Arkin, A.P.

    2011-04-01T23:59:59.000Z

    Sulphate-reducing microorganisms (SRMs) are a phylogenetically diverse group of anaerobes encompassing distinct physiologies with a broad ecological distribution. As SRMs have important roles in the biogeochemical cycling of carbon, nitrogen, sulphur and various metals, an understanding of how these organisms respond to environmental stresses is of fundamental and practical importance. In this Review, we highlight recent applications of systems biology tools in studying the stress responses of SRMs, particularly Desulfovibrio spp., at the cell, population, community and ecosystem levels. The syntrophic lifestyle of SRMs is also discussed, with a focus on system-level analyses of adaptive mechanisms. Such information is important for understanding the microbiology of the global sulphur cycle and for developing biotechnological applications of SRMs for environmental remediation, energy production, biocorrosion control, wastewater treatment and mineral recovery.

  2. Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity

    DOE Patents [OSTI]

    Premuzic, Eugene T. (East Moriches, NY); Lin, Mow (Rocky Point, NY)

    1996-02-20T23:59:59.000Z

    This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70.degree. C. to 90.degree. C., at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%.

  3. Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity

    DOE Patents [OSTI]

    Premuzic, E.T.; Lin, M.

    1996-02-20T23:59:59.000Z

    This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70 C to 90 C, at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%. 68 figs.

  4. Live Cell Chemical Profiling of Temporal Redox Dynamics in a Photoautotrophic Cyanobacterium

    SciTech Connect (OSTI)

    Sadler, Natalie C.; Melnicki, Matthew R.; Serres, Margrethe H.; Merkley, Eric D.; Chrisler, William B.; Hill, Eric A.; Romine, Margaret F.; Kim, Sangtae; Zink, Erika M.; Datta, Suchitra; Smith, Richard D.; Beliaev, Alex S.; Konopka, Allan; Wright, Aaron T.

    2014-01-01T23:59:59.000Z

    Protein reduction-oxidation (redox) modification is an important mechanism that allows microorganisms to sense environmental changes and initiate cellular responses. We have developed a quantitative chemical probe approach for live cell labeling of proteins that are sensitive to redox modifications. We utilize this in vivo strategy to identify 176 proteins undergoing ~5-10 fold dynamic redox change in response to nutrient limitation and subsequent replenishment in the photoautotrophic cyanobacterium, Synechococcus sp. PCC 7002. We detect redox changes in as little as 30 seconds after nutrient perturbation, and oscillations in reduction and oxidation for 60 minutes following the perturbation. Many of the proteins undergoing dynamic redox transformations participate in the major components for the production (photosystems and electron transport chains) or consumption (Calvin-Benson cycle and protein synthesis) of reductant and/or energy in photosynthetic organisms. Thus, our in vivo approach reveals new redox-susceptible proteins, in addition to validating those previously identified in vitro.

  5. The Stabilisation Potential of Individual and Mixed Assemblages of Natural Bacteria and Microalgae

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    The Stabilisation Potential of Individual and Mixed Assemblages of Natural Bacteria and Microalgae components (heterotrophic bacteria and autotrophic microalgae) in terms of their individual contribution benthic assemblages (prokaryotic, eukaryotic and mixed cultures) of bacteria and microalgae. Mag

  6. Determination of kinetic coefficients for the reduction and removal of uranium from water by the Desulfovibrio desulfuricans bacteria

    SciTech Connect (OSTI)

    Tucker, M.D.; Barton, L.L.; Thomson, B.M. [Sandia National Labs., Albuquerque, NM (United States)

    1996-12-31T23:59:59.000Z

    Uranium contamination of groundwater and surface water from abandoned uranium mill tailings piles is a serious concern in many areas of the western United States. U(VI) is soluble in water and, as a result, is relatively mobile in the environment. U(IV), however, is generally insoluble in water and, therefore, is not subject to aqueous transport. In recent years, researchers have discovered that certain microorganisms, such as the sulfate-reducing bacteria Desuffiovibrio desulfricans, can mediate the reduction of U(VI) to U(IV) by anaerobic respiration. Although the ability of this microorganism to reduce U(VI) has been studied in some detail by previous researchers, the kinetics of the reaction have not been characterized. The purpose of this research was to perform kinetic studies on Desuffiovibrio desulfricans during simultaneous reduction of sulfate and uranium and to determine the mineral phase of uranium after it has been reduced. The studies were conducted in a laboratory-scale chemostat under substrate-limited growth conditions with pyruvate as the substrate. The maximum rate of substrate utilization (k) was determined to be 4.70 days{sup -1} while the half-velocity constant (Ks) was 140 mg CODA. The yield coefficient (Y) was determined to be 0. 17 mg cells/mg COD while the endogenous decay coefficient (kd) was found to be 0.072 days{sup -1}. After reduction, U(IV) precipitated from solution in the uraninite (UO{sub 2}) phase as predicted by thermodynamics. Uranium removal efficiency as high as 90% was achieved in the chemostat.

  7. E-Print Network 3.0 - abundant culturable bacteria Sample Search...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Culturable bacteria in Summary: BGD 3, 765-778, 2006 Culturable bacteria in Himalayan ice S. Zhang et al. Title Page Abstract... Biogeosciences Discussions is the access...

  8. Bacteria Total Maximum Daily Load Task Force Final Report

    E-Print Network [OSTI]

    Jones, C. Allan; Wagner, Kevin; Di Giovanni, George; Hauck, Larry; Mott, Joanna; Rifai, Hanadi; Srinivasan, Raghavan; Ward, George; Wythe, Kathy

    for TMDL and Watershed Studies at Virginia Tech (http://www.tmdl.bse.vt.edu/outreach/C85/). Bacteria Indicator Tool (BIT) Another MB tool is the BIT provided by EPA (http://www.epa.gov/waterscience/ftp/basins/system/BASINS3/bit.htm). The BIT is a... spreadsheet that can be used to estimate the monthly accumulation rate of fecal coliform bacteria on four land uses (cropland, forested, built-up and pastureland). The tool also estimates the direct input of fecal coliform bacteria to streams from grazing...

  9. Sustainable Living Guide This sustainable living guide is the product of a class project for Geography 6250 at Memorial

    E-Print Network [OSTI]

    Oyet, Alwell

    St. John's Sustainable Living Guide #12;This sustainable living guide is the product of a class's Sustainable Living Guide. Memorial University of Newfoundland, St. John's, Newfoundland, Canada, 40 p. Any...........................................................................................................2-4 Sustainable Home Living

  10. Live with the Energy Innovation Hub Directors

    Broader source: Energy.gov [DOE]

    Tune in today, Tuesday, March 6, 2012, at 2:15pm EST for a live discussion with our three Energy Innovation Hubs directors.

  11. Nuclear Physics Technology Saves Lives | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Saves Lives January 11, 2006 Listen to this story Ribbon With early detection, breast cancer can often be treated successfully. There are over two million breast cancer survivors...

  12. Extending the Operating Lives of Materials

    ScienceCinema (OSTI)

    Paul Jablonski

    2010-09-01T23:59:59.000Z

    Metallurgist Paul Jablonski discusses his role in developing processes that extend the operating temperatures and operating lives of materials used in energy applications.

  13. Extending the Operating Lives of Materials

    SciTech Connect (OSTI)

    Paul Jablonski

    2010-01-26T23:59:59.000Z

    Metallurgist Paul Jablonski discusses his role in developing processes that extend the operating temperatures and operating lives of materials used in energy applications.

  14. Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1998-05-26T23:59:59.000Z

    Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  15. Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1997-12-16T23:59:59.000Z

    Bacteria which produce large amounts of cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  16. Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig (Lenoir City, TN)

    1997-12-16T23:59:59.000Z

    Bacteria which produce large amounts of cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  17. Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig (Lenoir City, TN)

    1998-01-01T23:59:59.000Z

    Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  18. Beating bacteria: Scientists work to understand and track bacteria in water

    E-Print Network [OSTI]

    Wythe, Kathy

    2010-01-01T23:59:59.000Z

    to Tier 2. Tier 2 is a one- to two-year effort with the follow- ing steps: 1) Collect targeted monitoring data to fill gaps in previously collected data 2) Conduct qualitative library-independent BST and limited library-dependent BST analysis... to determine whether humans and/or a few major classes of animals are sources 3) Develop simple spatially explicit or mass balance models of bacteria in the watershed 4) Analyze data After analysis of Tier 1 and Tier 2 data, the group may chose...

  19. A C. elegans-based foam for rapid on-site detection of residual live virus.

    SciTech Connect (OSTI)

    Negrete, Oscar A.; Branda, Catherine; Hardesty, Jasper O. E. (Sandia National Laboratories, Albuquerque, NM); Tucker, Mark David (Sandia National Laboratories, Albuquerque, NM); Kaiser, Julia N. (Global Product Management, Hilden, Germany); Kozina, Carol L.; Chirica, Gabriela S.

    2012-02-01T23:59:59.000Z

    In the response to and recovery from a critical homeland security event involving deliberate or accidental release of biological agents, initial decontamination efforts are necessarily followed by tests for the presence of residual live virus or bacteria. Such 'clearance sampling' should be rapid and accurate, to inform decision makers as they take appropriate action to ensure the safety of the public and of operational personnel. However, the current protocol for clearance sampling is extremely time-intensive and costly, and requires significant amounts of laboratory space and capacity. Detection of residual live virus is particularly problematic and time-consuming, as it requires evaluation of replication potential within a eukaryotic host such as chicken embryos. The intention of this project was to develop a new method for clearance sampling, by leveraging Sandia's expertise in the biological and material sciences in order to create a C. elegans-based foam that could be applied directly to the entire contaminated area for quick and accurate detection of any and all residual live virus by means of a fluorescent signal. Such a novel technology for rapid, on-site detection of live virus would greatly interest the DHS, DoD, and EPA, and hold broad commercial potential, especially with regard to the transportation industry.

  20. Top 10 plant pathogenic bacteria in molecular plant pathology.

    E-Print Network [OSTI]

    2012-01-01T23:59:59.000Z

    Foster, G.D. (2012) The top 10 fungal pathogens in molecularBLACKWELL PUBLISHING LTD Top 10 plant pathogenic bacteriaC. and Foster, G.D. (2011) Top 10 plant viruses in molecular

  1. Bacteria mix it up at the microscopic level | Argonne National...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by 100-fold. A second surprise came when the researchers began to measure the viscosity of the fluid in which the bacteria swam. Viscosity measures how easily a fluid...

  2. Micro-scale interactions between chemotactic bacteria and algae

    E-Print Network [OSTI]

    Vahora, Nisha

    2010-01-01T23:59:59.000Z

    Traditional views of marine environments describe the ocean pelagic zone as a homogeneous nutrient-poor environment. Heterotrophic marine bacteria that have evolved high-energy mechanisms for swimming abilities and sensing ...

  3. Argonne scientists use bacteria to power simple machines | Argonne...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    use bacteria to power simple machines December 16, 2009 Tweet EmailPrint ARGONNE, Ill. - Scientists at the U.S. Department of Energy's (DOE) Argonne National Laboratory and...

  4. Bacteria Total Maximum Daily Load Task Force Final Report 

    E-Print Network [OSTI]

    Jones, C. Allan; Wagner, Kevin; Di Giovanni, George; Hauck, Larry; Mott, Joanna; Rifai, Hanadi; Srinivasan, Raghavan; Ward, George; Wythe, Kathy

    2009-01-01T23:59:59.000Z

    In September 2006, the Texas Commission on Environmental Quality (TCEQ) and Texas State Soil and Water Conservation Board (TSSWCB) charged a seven-person Bacteria Total Maximum Daily Load (TMDL) Task Force with: * examining approaches...

  5. Bacteria recovered from endometritis and pyometra in the beef cow 

    E-Print Network [OSTI]

    Mikulec, Rashel Thi

    1999-01-01T23:59:59.000Z

    One hundred and one uteri from beef cows with pyometra were collected from a slaughterhouse. Samples of uterine exudate were cultured for aerobic, microaerophilic, and anaerobic bacteria, and also tested for Trichomonas spp. A section of uterine...

  6. Just How Fast Can Bacteria Grow? It Depends. | EMSL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    coli compared with E. coli that had been adapted for growth on either lactate or glycerol media. Bacteria are among the fastest reproducing organisms in the world, doubling every...

  7. Spatially-Resolved Analysis of Glycolipids and Metabolites in Living Synechococcus sp. PCC7002 Using Nanospray Desorption Electrospray Ionization

    SciTech Connect (OSTI)

    Lanekoff, Ingela T.; Geydebrekht, Oleg V.; Pinchuk, Grigoriy E.; Konopka, Allan; Laskin, Julia

    2013-04-07T23:59:59.000Z

    Microorganisms release a diversity of organic compounds that couple interspecies metabolism, enable communication, or provide benefits to other microbes. Increased knowledge of microbial metabolite production will contribute to understanding of the dynamic microbial world and can potentially lead to new developments in drug discovery, biofuel production, and clinical research. Nanospray desorption electrospray ionization (nano-DESI) is an ambient ionization technique that enables detailed chemical characterization of molecules from a specific location on a surface without special sample pretreatment. Due to its ambient nature, living bacterial colonies growing on agar plates can be rapidly and non-destructively analyzed. We performed spatially resolved nano-DESI analysis of living Synechococcus sp. PCC 7002 colonies on agar plates. We use high resolution mass spectrometry and MS/MS analysis of the living Synechococcus sp. PCC 7002 colonies to detect metabolites and lipids, and confirm their identities. We found that despite the high salt content of the agar (osmolarity ca. 700 mM), nano-DESI analysis enables detailed characterization of metabolites produced by the colony. Using this technique, we identified several glycolipids found on the living colonies and examined the effect of the age of the colony on the chemical gradient of glucosylglycerol secreted onto agar.

  8. Petroleum hydrocarbon-degrading bacteria in the Galveston Bay system

    E-Print Network [OSTI]

    Schropp, Steven James

    1979-01-01T23:59:59.000Z

    of Department) (Membge' ) I, (Member) December, 1979 ABSTRACT Petroleum Hydrocarbon-Degrading Bacteria in the Galveston Bay System {December 1979) Steven James Schropp: B. S. , Texas A&M University Co-Chairmen of Advisory Committee: Dr. John R. Schwarz... about the distribution and abundance of petroleum hydrocarbon-degrad- ing bacteria in the Galveston Bay system. Several parts of the Galveston Bay system were sampled during this study. Petroleum input to the study area ranged from relatively small...

  9. Petroleum hydrocarbon-degrading bacteria in the Galveston Bay system 

    E-Print Network [OSTI]

    Schropp, Steven James

    1979-01-01T23:59:59.000Z

    PETROLEUM HYDRQCARBOiV-DEGRADING BACTERIA IN THE GALVESTON BAY SYSTEM A Thesis by STEVEN JAMES SCHROPP Submitted to the Graduate College of Texas ASM University in partial fulfillment of the requirement for the degree of MASTER OF SCIFNCE... December 1979 Major Subject: Biology PETROLEUM HYDROCARBON-DEGRADING BACTERIA IN THE GALVESTON BAY SYSTEM A Thesis by STEVEN JAMES SCHROPP Approved as to style and content by: (Co-Chairman of Committee) '( ~CA. ( -Chairman of Committee) (Head...

  10. s Earth and environment s Living resources

    E-Print Network [OSTI]

    4 s Earth and environment s Living resources s Societies and health s Expertise and consulting of this trend is the acquisition, on a joint proposal from the Earth and Environment department and the Living phenomena so as to improve forecasting of the attendant hazards. The earth's crust: processes and natural

  11. INVEST IN YOUR BONES Living with Osteoporosis

    E-Print Network [OSTI]

    INVEST IN YOUR BONES Living with Osteoporosis Leaflet 5 Living with osteoporosis can be done environment safe to avoid falls. Early detection of bone loss or osteoporosis is now possible with bone to be most effective in reducing bone loss during the five to ten years following menopause, when bone loss

  12. Living Village Kioni Village, Ithaca, Greece

    E-Print Network [OSTI]

    Zaferatos, Nicholas C.

    University | 2010 #12;Living | Village Kioni Village Ithaca, Greece A X A A Sustainable Ithaca ProgramLiving Village Kioni Village, Ithaca, Greece A X A A Nicholas Zaferatos Western Washington Zaferatos, Sustainable Ithaca Faculty Program Director, at nicholas.zaferatos@wwu.edu #12;Table of Contents

  13. 1 | P a g e Living a Digital Life Camp Camp: Living a Digital Life

    E-Print Network [OSTI]

    Seldin, Jonathan P.

    , captchas, social media, MMORPG -- this camp explores the digital world from both the user1 | P a g e Living a Digital Life Camp Camp: Living a Digital Life August 11- 15 for participants 11 - 13 years old General description: We live in a digital world, where the ground shifts faster

  14. Endosymbiosis In Statu Nascendi: Close Phylogenetic RelationshipBetween Obligately Endosymbiotic and Obligately Free-LivingPolynucleobacter Strains (Betaproteobacteria)

    SciTech Connect (OSTI)

    Vannini, Claudia; Pockl, Matthias; Petroni, Giulio; Wu, Qinglong; Lang, Elke; Stackebrandt, Erko; Schrallhammer, Martina; Richardson, PaulM.; Hahn, Martin W.

    2006-07-21T23:59:59.000Z

    Bacterial strains affiliated to the phylogenetically shallowsubcluster C (PnecC) of the 28 Polynucleobacter cluster, which ischaracterized by a minimal 16S rRNA gene sequence similarity of approx.98.5 percent, have been reported to occur as obligate endosymbionts of 30ciliates (Euplotes spp.), as well as to occur as free-living cells in thepelagic zone of freshwater habitats. We investigated if these two groupsof closely related bacteria represent 32 strains fundamentally differingin lifestyle, or if they simply represent different stages of afacultative endosymbiotic lifestyle. The phylogenetic analysis of 16SrRNA gene and 16S34 23S ITS sequences of five endosymbiont strains fromtwo different Euplotes species and 40 pure culture strains demonstratedhost-species-specific clustering of the endosymbiont 36 sequences withinthe PnecC subcluster. The sequences of the endosymbionts showedcharacteristics indicating an obligate endosymbiotic lifestyle.Cultivation experiments 38 revealed fundamental differences inphysiological adaptations, and determination of the genome sizesindicated a slight size reduction in endosymbiotic strains. We concludethat the 40 two groups of PnecC bacteria represent obligately free-livingand obligately endosymbiotic strains, respectively, and do not representdifferent stages of the same complex lifecycle. 42 These closely relatedstrains occupy completely separated ecological niches. To our bestknowledge, this is the closest phylogenetic relationship between obligateendosymbionts and 44 obligately free-living bacteria everrevealed.

  15. Influence of microorganisms on the oxidation state distribution of multivalent actinides under anoxic conditions

    SciTech Connect (OSTI)

    Reed, Donald Timothy [Los Alamos National Laboratory; Borkowski, Marian [Los Alamos National Laboratory; Lucchini, Jean - Francois [Los Alamos National Laboratory; Ams, David [Los Alamos National Laboratory; Richmann, M. K. [Los Alamos National Laboratory; Khaing, H. [Los Alamos National Laboratory; Swanson, J. S. [Los Alamos National Laboratory

    2010-12-10T23:59:59.000Z

    The fate and potential mobility of multivalent actinides in the subsurface is receiving increased attention as the DOE looks to cleanup the many legacy nuclear waste sites and associated subsurface contamination. Plutonium, uranium and neptunium are the near-surface multivalent contaminants of concern and are also key contaminants for the deep geologic disposal of nuclear waste. Their mobility is highly dependent on their redox distribution at their contamination source as well as along their potential migration pathways. This redox distribution is often controlled, especially in the near-surface where organic/inorganic contaminants often coexist, by the direct and indirect effects of microbial activity. Under anoxic conditions, indirect and direct bioreduction mechanisms exist that promote the prevalence of lower-valent species for multivalent actinides. Oxidation-state-specific biosorption is also an important consideration for long-term migration and can influence oxidation state distribution. Results of ongoing studies to explore and establish the oxidation-state specific interactions of soil bacteria (metal reducers and sulfate reducers) as well as halo-tolerant bacteria and Archaea for uranium, neptunium and plutonium will be presented. Enzymatic reduction is a key process in the bioreduction of plutonium and uranium, but co-enzymatic processes predominate in neptunium systems. Strong sorptive interactions can occur for most actinide oxidation states but are likely a factor in the stabilization of lower-valent species when more than one oxidation state can persist under anaerobic microbiologically-active conditions. These results for microbiologically active systems are interpreted in the context of their overall importance in defining the potential migration of multivalent actinides in the subsurface.

  16. An Approach for Assessing the Signature Quality of Various Chemical Assays when Predicting the Culture Media Used to Grow Microorganisms

    SciTech Connect (OSTI)

    Holmes, Aimee E.; Sego, Landon H.; Webb-Robertson, Bobbie-Jo M.; Kreuzer, Helen W.; Anderson, Richard M.; Unwin, Stephen D.; Weimar, Mark R.; Tardiff, Mark F.; Corley, Courtney D.

    2013-02-01T23:59:59.000Z

    We demonstrate an approach for assessing the quality of a signature system designed to predict the culture medium used to grow a microorganism. The system was comprised of four chemical assays designed to identify various ingredients that could be used to produce the culture medium. The analytical measurements resulting from any combination of these four assays can be used in a Bayesian network to predict the probabilities that the microorganism was grown using one of eleven culture media. We evaluated combinations of the signature system by removing one or more of the assays from the Bayes network. We measured and compared the quality of the various Bayes nets in terms of fidelity, cost, risk, and utility, a method we refer to as Signature Quality Metrics

  17. Metallomics of two microorganisms relevant to heavy metal bioremediation reveal fundamental differences in metal assimilation and utilization

    SciTech Connect (OSTI)

    Lancaster, Andrew [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Menon, Angeli [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Scott, Israel [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Poole, Farris [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Vaccaro, Brian [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Thorgersen, Michael P [ORNL] [ORNL; Geller, Jil [Lawrence Berkeley National Laboratory (LBNL)] [Lawrence Berkeley National Laboratory (LBNL); Hazen, Terry C [ORNL] [ORNL; Hurt, Jr., Richard Ashley [ORNL; Brown, Steven D [ORNL] [ORNL; Elias, Dwayne A [ORNL] [ORNL; Adams, Michael W. W. [University of Georgia, Athens, GA] [University of Georgia, Athens, GA

    2014-01-01T23:59:59.000Z

    Although as many as half of all proteins are thought to require a metal cofactor, the metalloproteomes of microorganisms remain relatively unexplored. Microorganisms from different environments are likely to vary greatly in the metals that they assimilate, not just among the metals with well-characterized roles but also those lacking any known function. Herein we investigated the metal utilization of two microorganisms that were isolated from very similar environments and are of interest because of potential roles in the immobilization of heavy metals, such as uranium and chromium. The metals assimilated and their concentrations in the cytoplasm of Desulfovibrio vulgaris strain Hildenborough (DvH) and Enterobacter cloacae strain Hanford (EcH) varied dramatically, with a larger number of metals present in Enterobacter. For example, a total of 9 and 19 metals were assimilated into their cytoplasmic fractions, respectively, and DvH did not assimilate significant amounts of zinc or copper whereas EcH assimilated both. However, bioinformatic analysis of their genome sequences revealed a comparable number of predicted metalloproteins, 813 in DvH and 953 in EcH. These allowed some rationalization of the types of metal assimilated in some cases (Fe, Cu, Mo, W, V) but not in others (Zn, Nd, Ce, Pr, Dy, Hf and Th). It was also shown that U binds an unknown soluble protein in EcH but this incorporation was the result of extracellular U binding to cytoplasmic components after cell lysis.

  18. Biodesulfurization techniques: Application of selected microorganisms for organic sulfur removal from coals. Final report

    SciTech Connect (OSTI)

    Elmore, B.B.

    1993-08-01T23:59:59.000Z

    As an alternative to post-combustion desulfurization of coal and pre-combustion desulfurization using physicochemical techniques, the microbial desulfurization of coal may be accomplished through the use of microbial cultures that, in an application of various microbial species, may remove both the pyritic and organic fractions of sulfur found in coal. Organisms have been isolated that readily depyritize coal but often at prohibitively low rates of desulfurization. Microbes have also been isolated that may potentially remove the organic-sulfur fraction present in coal (showing promise when acting on organic sulfur model compounds such as dibenzothiophene). The isolation and study of microorganisms demonstrating a potential for removing organic sulfur from coal has been undertaken in this project. Additionally, the organisms and mechanisms by which coal is microbially depyritized has been investigated. Three cultures were isolated that grew on dibenzothiophene (DBT), a model organic-sulfur compound, as the sole sulfur source. These cultures (UMX3, UMX9, and IGTS8) also grew on coal samples as the sole sulfur source. Numerous techniques for pretreating and ``cotreating`` coal for depyritization were also evaluated for the ability to improve the rate or extent of microbial depyritization. These include prewashing the coal with various solvents and adding surfactants to the culture broth. Using a bituminous coal containing 0.61% (w/w) pyrite washed with organic solvents at low slurry concentrations (2% w/v), the extent of depyritization was increased approximately 25% in two weeks as compared to controls. At slurry concentrations of 20% w/v, a tetrachloroethylene treatment of the coal followed by depyritization with Thiobacillus ferrooxidans increased both the rate and extent of depyritization by approximately 10%.

  19. campus as living laboratory institutional Sustainability Plan

    E-Print Network [OSTI]

    Calgary, University of

    operations campus as living laboratory diversity campus waste institutional Sustainability Plan eliminate priorities energy research 35%ofwastedivertedSTARSSilver 275 sustainability courses investment 60 accessibility faculty leadership 100+ sustainability research projects over $3.8 million in bursaries Calgary

  20. Adapting Your Home for More Accessible Living

    E-Print Network [OSTI]

    Harris, Janie

    2007-04-27T23:59:59.000Z

    It is important for people to live comfortably and independently in their homes. Homes can be adapted to aid people with various disabilities. This publication explains how to make such adaptations for people with vision loss, hearing loss, problems...

  1. Living With Roommates a practical guide

    E-Print Network [OSTI]

    Loudon, Catherine

    /Cleaning (personal living area) y Mail pick-up y Pet peeves y Smoking y Food (sharing/cooking) y Room temperature y Decorations y Alcohol (consumption values & practices) y Telephone/payment/usage/messages Suggested Topics

  2. The Live Room : transducing resonant architectures

    E-Print Network [OSTI]

    Bain, Mark, 1966-

    1998-01-01T23:59:59.000Z

    The Live Room is a temporary site specific installation presented in building N 51, room 117 on the MIT campus on May 7, 1998 and concluded on June 10, 1998. Using small acoustic intensifying equipment which mount directly ...

  3. Dynamical real numbers and living systems

    E-Print Network [OSTI]

    Dhurjati Prasad Datta

    2010-01-11T23:59:59.000Z

    Recently uncovered second derivative discontinuous solutions of the simplest linear ordinary differential equation define not only an nonstandard extension of the framework of the ordinary calculus, but also provide a dynamical representation of the ordinary real number system. Every real number can be visualized as a living cell -like structure, endowed with a definite evolutionary arrow. We discuss the relevance of this extended calculus in the study of living systems. We also present an intelligent version of the Newton's first law of motion.

  4. Bacteria transport through porous media. Annual report, December 31, 1984

    SciTech Connect (OSTI)

    Yen, T.F.

    1986-09-01T23:59:59.000Z

    The following five chapters in this report have been processed separately for inclusion in the Energy Data Base: (1) theoretical model of convective diffusion of motile and non-motile bacteria toward solid surfaces; (2) interfacial electrochemistry of oxide surfaces in oil-bearing sands and sandstones; (3) effects of sodium pyrophosphate additive on the ''huff and puff''/nutrient flooding MEOR process; (4) interaction of Escherichia coli B, B/4, and bacteriophage T4D with Berea sandstone rock in relation to enhanced oil recovery; and (5) transport of bacteria in porous media and its significance in microbial enhanced oil recovery.

  5. In Situ Live Cell Sensing of Multiple Nucleotides Exploiting...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In Situ Live Cell Sensing of Multiple Nucleotides Exploiting DNARNA Aptamers and Graphene Oxide Nanosheets. In Situ Live Cell Sensing of Multiple Nucleotides Exploiting DNARNA...

  6. Senior Adviser Richard Kauffman to Host Live Chat on Renewable...

    Office of Environmental Management (EM)

    Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and Deployment Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and...

  7. Enumeration and characterization of microorganisms associated with the uranium ore deposit at Cigar Lake, Canada; Informal report

    SciTech Connect (OSTI)

    Francis, A.J.; Joshi-Tope, G.; Gillow, J.B.; Dodge, C.J.

    1994-03-01T23:59:59.000Z

    The high-grade uranium deposit at Cigar Lake, Canada, is being investigated as a natural analog for the disposal of nuclear fuel waste. Geochemical aspects of the site have been studied in detail, but the microbial ecology has not been fully investigated. Microbial populations in an ore sample and in groundwater samples from the vicinity of the ore zone were examined to determine their effect on uranium mobility. Counts of the total number of bacteria and of respiring bacteria were obtained by direct microscopy, and the viable aerobic and anaerobic bacteria were assessed as colony forming units (CFUs) by the dilution plating technique. In addition, the population distribution of denitrifiers, fermenters, iron- and sulfur-oxidizers, iron- and sulfate-reducers, and methanogens was determined by the most probable number (MPN) technique.

  8. Physiologically anaerobic microorganisms of the deep subsurface. Final performance report, June 1, 1990--August 31, 1993

    SciTech Connect (OSTI)

    Stevens, S.E. Jr.; Chung, K.T.

    1993-10-01T23:59:59.000Z

    Anaerobic bacteria were isolated from deep subsurface sediment samples taken at study sites in Idaho (INEL) and Washington (HR) by culturing on dilute and concentrated medium. Morphologically distinct colonies were purified, and their responses to 21 selected physiological tests were determined. Although the number of isolates was small (18 INEL, 27 HR) some general patterns could be determined. Most strains could utilize all the carbon sources, however the glycerol and melizitose utilization was positive for 50% or less of the HR isolates. Catalase activity (27.78% at INEL, 74.07% at HR) and tryptophan metabolism (11.12% at INEL, 40.74% at HR) were significantly different between the two study sites. MPN and viable counts indicate that sediments near the water table yield the greatest numbers of anaerobes. Deeper sediments also appear to be more selective with the greatest number of viable counts on low-nutrient mediums. Likewise, only strictly obligate anaerobes were found in the deepest sediment samples. Selective media indicated the presence of methanogens, acetogens, and sulfate reducers at only the HR site.

  9. Effects of selected thermophilic microorganisms on crude oils at elevated temperatures and pressures. 1991 annual report

    SciTech Connect (OSTI)

    Premuzic, E.T.; Lin, M.S.

    1993-10-01T23:59:59.000Z

    During the past several years, a considerable amount of work has been carried out showing that microbially enhanced oil recovery (MEOR) is promising and the resulting biotechnology may be deliverable. In this laboratory systematic studies are being conducted which deal with the effects of thermophilic and thermoadapted bacteria on the chemical and physical properties of selected types of crude oils at elevated temperatures and pressures. Particular attention is being paid to heavy crude oils such as Boscan and Cerro Negro (Venezuela), Monterey (California) and those from Alabama and Arkansas. Current studies indicate that during the biotreatment several properties of crude oils are affected. The oils are (1) emulsified; (2) acidified; (3) there is a qualitative and quantitative change in light and heavy fractions of the crudes; (4) there are chemical changes in fractions containing sulfur compounds; (5) there is an apparent solubilization of trace metals; and (6) the qualitative and quantitative chemical and physical changes appear to be microbial species dependent. Effects on heavy crude oils are also compared to those on lighter oils such as oils from the Wyoming petroleum reserve. Microbial oil interactions are monitored routinely by a consortium of analytical techniques which are continuously upgraded and are capable of multiparameter analysis. The results generated in fiscal year 1991, describing (1) through (6), are presented and discussed in this report.

  10. Bacteria beneath the West Antarctic Ice Sheet Brian Lanoil,1

    E-Print Network [OSTI]

    Priscu, John C.

    Bacteria beneath the West Antarctic Ice Sheet Brian Lanoil,1 * Mark Skidmore,1 John C. Priscu,2, particularly those that lie beneath polar ice sheets, are beginning to be recog- nized as an important part Vostok, Antarctica, no sub-ice sheet environments have been shown to support microbial ecosystems. Here

  11. Methods for targetted mutagenesis in gram-positive bacteria

    DOE Patents [OSTI]

    Yang, Yunfeng

    2014-05-27T23:59:59.000Z

    The present invention provides a method of targeted mutagenesis in Gram-positive bacteria. In particular, the present invention provides a method that effectively integrates a suicide integrative vector into a target gene in the chromosome of a Gram-positive bacterium, resulting in inactivation of the target gene.

  12. Measurement of chlorite dismutase activities in perchlorate respiring bacteria

    E-Print Network [OSTI]

    concentration (0.6 mM) to four other perchlorate respiring bacteria (PRB), and to one non-PRB (Pseudomonas aeruginosa). Chlorate-grown cultures of the five PRB strains had CD activities ranging from 25 to 50 U/mg of cell dry weight (DW), while aerobically grown cultures of the PRB had much lower CD activities (0.5­4 U

  13. Magnetic Torque of Microfabricated Elements and Magnetotactic Bacteria

    E-Print Network [OSTI]

    Lars Zondervan; Özlem Sardan Sukas; Islam S. M. Khalil; Marc P. Pichel; Sarthak Misra; Leon Abelmann

    2014-08-07T23:59:59.000Z

    We present a thorough theoretical analysis of the magnetic torque on microfabricated elements with dimensions in the range of 100 to 500 {\\mu}m and magneto-somes of magnetotactic bacteria of a few {\\mu}m length. We derive simple equations for field dependent torque and magnetic shape anisotropy that can be readily used to replace the crude approximations commonly used. We illustrate and verify the theory on microfabricated elements and magnetotactic bacteria, by field depedent torque magnetometry and by observing their rotation in water under application of a rotating magnetic field. The maximum rotation frequency of the largest microfabricated elements agrees within error boundaries with theory. For smaller, and especially thinner, elements the measured frequencies are a factor of three to four too low. We suspect this is caused by incomplete saturation of the magnetisation in the elements, which is not incorporated in our model. The maximum rotation frequency of magnetotactic bacteria agrees with our model within error margins, which are however quite big due to the large spread in bacteria morphology. The model presented provides a solid basis for the analysis of experiments with magnetic objects in liquid, which is for instance the case in the field of medical microrobotics.

  14. Films of bacteria at interfaces: three stages of behaviour

    E-Print Network [OSTI]

    Liana Vaccari; Daniel Allan; Nima Sharifi-Mood; Aayush Singh; Robert Leheny; Kathleen Stebe

    2015-03-25T23:59:59.000Z

    Bacterial attachment to a fluid interface can lead to the formation of a film with physicochemical properties that evolve with time. We study the time evolution of interface (micro)mechanics for interfaces between oil and bacterial suspensions by following the motion of colloidal probes trapped by capillarity to determine the interface microrheology. Initially, active bacteria at and near the interface drive superdiffusive motion of the colloidal probes. Over timescales of minutes, the bacteria form a viscoelastic film which we discuss as a quasi-two-dimensional, active, glassy system. To study late stage mechanics of the film, we use pendant drop elastometry. The films, grown over tens of hours on oil drops, are expanded and compressed by changing the drop volume. For small strains, by modeling the films as 2D Hookean solids, we estimate the film elastic moduli, finding values similar to those reported in the literature for the bacteria themselves. For large strains, the films are highly hysteretic. Finally, from wrinkles formed on highly compressed drops, we estimate film bending energies. The dramatic restructuring of the interface by such robust films has broad implications, e.g. in the study of active colloids, in understanding the community dynamics of bacteria, and in applied settings including bioremediation.

  15. Method for establishing the presence of salmonella bacteria in eggs

    DOE Patents [OSTI]

    Johnston, Roger G. (Los Alamos, NM); Sinha, Dipen N. (Los Alamos, NM)

    1995-01-01T23:59:59.000Z

    Measurement of the acoustical resonances in eggs is shown to provide a rapid, noninvasive technique for establishing the presence of Salmonella bacteria. The technique is also sensitive to yolk puncture, shell cracks, and may be sensitive to other yolk properties and to egg freshness. Remote characterization, potentially useful for characterizing large numbers of eggs, has been demonstrated.

  16. Energetics of active fluctuations in living cells

    E-Print Network [OSTI]

    Étienne Fodor; Kiyoshi Kanazawa; Hisao Hayakawa; Paolo Visco; Frédéric van Wijland

    2014-06-06T23:59:59.000Z

    The nonequilibrium activity taking place in a living cell can be monitored with a tracer embedded in the medium. While microrheology experiments based on optical manipulation of such probes have become increasingly standard, we put forward a number of experiments with alternative protocols that, we claim, will provide new insight into the energetics of active fluctuations. These are based on either performing thermodynamic--like cycles in control-parameter space, or on determining response to external perturbations of the confining trap beyond simple translation. We illustrate our proposals on an active itinerant Brownian oscillator modeling the dynamics of a probe embedded in a living medium.

  17. Live Webinar on Better Buildings Challenge: Public-Sector Update

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webinar titled "Better Buildings Challenge: Public-Sector Update."

  18. housing.ucdavis.edu Living at UC Davis

    E-Print Network [OSTI]

    Hernes, Peter J.

    housing.ucdavis.edu Living at UC Davis Residence Halls Living at UC Davis STUDENT HOUSING #12;2 Every year, Student Housing is home to over 6000 UC Davis students, most of whom are first-year freshmen and transfer students who live in the residence halls. Living in Student Housing is not required; however, most

  19. Biofilms as sources of fecal bacteria contamination in the stormwater drainage system in Singapore

    E-Print Network [OSTI]

    Burkhart, Tsung Hwa (Tsung Hwa Sophia)

    2013-01-01T23:59:59.000Z

    A study was performed to examine a possible source of fecal bacteria contamination originating from within the stormwater drainage system in Singapore. The extent of fecal bacteria presence in storm drain biofilms was ...

  20. Development of Methodology and Characterization of Ruminal Lipase-Producing Bacteria In Vitro 

    E-Print Network [OSTI]

    Edwards, Holly Danielle

    2012-07-16T23:59:59.000Z

    nutrients into ruminant produced foods. Three experiments were conducted to learn how to better enrich, isolate and study lipolytic bacteria from the rumen while providing further characterization of four prominent lipase-producing bacteria that are known...

  1. The Association of Virulent Vibrio Spp. Bacteria on Gafftopsail and Hardhead Catfish in Galveston Bay 

    E-Print Network [OSTI]

    Gilbert, Leslie Deanne

    2011-10-21T23:59:59.000Z

    Vibrio vulnificus (Vv) and V. parahaemolyticus (Vp) are gram negative, halophilic bacteria that occur naturally in estuarine waters of Galveston Bay. Both bacteria have the potential to cause infections in humans either via consumption or direct...

  2. Live Streaming with Gossip Maxime Monod

    E-Print Network [OSTI]

    Guerraoui, Rachid

    ;2 TV ordinaire: tout en HD Live streaming Une source produit du contenu multimédia (un flux) n clients (n large) broadcasting diffusion ... ... ... IP TV, Web TV, P2P TV, ... vs 192K requêtes/jour 78K que le premier #12;·Gossip++Environment contraint ·HEAPEnvironnement hétérogène ·LiFT

  3. Quality health plans & benefits Healthier living

    E-Print Network [OSTI]

    to the Affordable Care Act, also known as the health care reform law, will comply with requirements for WomenQuality health plans & benefits Healthier living Financial well-being Intelligent solutions 05's Preventive Health Services. This means that for women with reproductive capacity, certain women

  4. Hermeneutics by the Living Anton Markos

    E-Print Network [OSTI]

    Markos, Anton

    EDITORIAL Hermeneutics by the Living Anton Markos Received: 20 January 2010 /Accepted: 22 March by introducing hermeneutics? After all, semiotics has already colonized the field, and biosemioticians of different "denominations" flourish at their annual meetings.1 Is not, after all, hermeneutics but an older

  5. living and learning Department of Residential Life

    E-Print Network [OSTI]

    Missouri-Columbia, University of

    living and learning Accessible housing at Mizzou Department of Residential Life University is important to us. The Department of Residential Life at MU is committed to providing and improving accessible spaces for students with disabilities. Residential Life will help provide appropriate housing

  6. MCM LTER METADATA FILE TITLE: Bacteria enumeration in lakes of the McMurdo Dry Valleys

    E-Print Network [OSTI]

    Priscu, John C.

    with a five-liter Niskin bottle during normal LTER limnological sampling. Sub-samples for bacteria enumeration are decanted into a 1 L amber Nalgene bottle. 18 mL bacteria samples are pipetted from the amber Nalgene bottle into new 20 ml glass scintillation vials for each depth. Bacteria samples are preserved by adding 0.5 ml

  7. PCR detection of groundwater bacteria associated with colloidal transport

    SciTech Connect (OSTI)

    Cruz-Perez, P.; Stetzenbach, L.D.; Alvarez, A.J.

    1996-02-29T23:59:59.000Z

    Colloidal transport may increase the amount of contaminant material than that which could be transported by water flow alone. The role of colloids in groundwater contaminant transport is complicated and may involve many different processes, including sorption of elements onto colloidal particles, coagulation/dissolution, adsorption onto solid surfaces, filtration, and migration. Bacteria are known to concentrate minerals and influence the transport of compounds in aqueous environments and may also serve as organic colloids, thereby influencing subsurface transport of radionuclides and other contaminants. The initial phase of the project consisted of assembling a list of bacteria capable of sequestering or facilitating mineral transport. The development and optimization of the PCR amplification assay for the detection of the organisms of interest, and the examination of regional groundwaters for those organisms, are presented for subsequent research.

  8. Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio

    SciTech Connect (OSTI)

    Chhabra, Swapnil R; Keller, Kimberly L.; Wall, Judy D.

    2011-03-15T23:59:59.000Z

    Sulfate reducing bacteria are physiologically important given their nearly ubiquitous presence and have important applications in the areas of bioremediation and bioenergy. This chapter provides details on the steps used for homologous-recombination mediated chromosomal manipulation of Desulfovibrio vulgaris Hildenborough, a well-studied sulfate reducer. More specifically, we focus on the implementation of a 'parts' based approach for suicide vector assembly, important aspects of anaerobic culturing, choices for antibiotic selection, electroporation-based DNA transformation, as well as tools for screening and verifying genetically modified constructs. These methods, which in principle may be extended to other sulfate-reducing bacteria, are applicable for functional genomics investigations, as well as metabolic engineering manipulations.

  9. Development of combinatorial bacteria for metal and radionuclide bioremediation

    SciTech Connect (OSTI)

    A. C. Matin, Ph. D.

    2006-06-15T23:59:59.000Z

    The grant concerned chromate [Cr(VI)] bioremediation and it was our aim from the outset to construct individual bacterial strains capable of improved bioremediation of multiple pollutants and to identify the enzymes suited to this end. Bacteria with superior capacity to remediate multiple pollutants can be an asset for the cleanup of DOE sites as they contain mixed waste. I describe below the progress made during the period of the current grant, providing appropriate context.

  10. Reduction of trichloroethylene in a model aquifer with methanotrophic bacteria 

    E-Print Network [OSTI]

    Hicks, Duane Dee

    1990-01-01T23:59:59.000Z

    REDUCTION OF TRICHLOROETHYLENE IN A MODEL AQUIFER WITH METHANOTROPHIC BACTERIA A Thesis by Duane Dee Hicks Submitted to the Office of Graduate Studies of Texas ARM University in partial fullfillment of the requirements for thc degree... of MASTER OF SCIENCE December 1990 Major Subject: Civil Engineering REDUCTION OF TRICHLOROETHYLENE IN A MODEL AQUIFER WITH METHANOTROPHIC BACTEPslA A Thesis by Duane Dec Hicks Approved as to style and content by Bill Batchclor (Chair of Committee...

  11. Reduction of trichloroethylene in a model aquifer with methanotrophic bacteria

    E-Print Network [OSTI]

    Hicks, Duane Dee

    1990-01-01T23:59:59.000Z

    for Methane Mineralization by Methanotrophic Bacteria ? Enzyme Reactions are a) Methane Monooxygenase, b) Methanol Dehydrogenase, c) Formaldehyde Dehydrogenase, d) Formate Dchydrogenase (Adapted From Dalton and Leak, 1985) . . . . . 9 FIG. 4 Proposed..., which is less efficient. Type II obligate methanotrophs using the serine pathway have been the focus of most of the recent methanotrophic bacterial research. Leak et al. (1985) found that different species of methane utilizers not only differ...

  12. Emergent patterns of diversity and dynamics in natural populations of planktonic Vibrio bacteria

    E-Print Network [OSTI]

    Thompson, Janelle Renée, 1976-

    2005-01-01T23:59:59.000Z

    Despite the importance of microorganisms for global and engineering processes, currently lacking is a theoretical framework to describe how the structure of a microbial assemblage translates an environmental condition into ...

  13. Universitt Wien News > Presse > Aktuelle Pressemeldungen > Think big! Bacteria breach cell division size limit

    E-Print Network [OSTI]

    , but much shorter (~ 2 micrometer), Escherichia coli", states Phd student Nika Pende with amazement "What we in the renowned journal Nature Communications. "The microorganisms thriving on the surface of marine tropical

  14. The future is now: single-cell genomics of bacteria and archaea

    E-Print Network [OSTI]

    Blainey, Paul C.

    Interest in the expanding catalog of uncultivated microorganisms, increasing recognition of heterogeneity among seemingly similar cells, and technological advances in whole-genome amplification and single-cell manipulation ...

  15. Oxidation and methylation of dissolved elemental mercury by anaerobic bacteria

    SciTech Connect (OSTI)

    Hu, Haiyan [ORNL] [ORNL; Lin, Hui [ORNL] [ORNL; Zheng, Wang [ORNL] [ORNL; Tomanicek, Stephen J [ORNL] [ORNL; Johs, Alexander [ORNL] [ORNL; Feng, Xinbin [ORNL] [ORNL; Elias, Dwayne A [ORNL] [ORNL; Liang, Liyuan [ORNL] [ORNL; Liang, Liyuan [ORNL] [ORNL; Gu, Baohua [ORNL] [ORNL

    2013-01-01T23:59:59.000Z

    Methylmercury is a neurotoxin that poses significant health risks to humans. Some anaerobic sulphate- and iron-reducing bacteria can methylate oxidized forms of mercury, generating methylmercury1-4. One strain of sulphate-reducing bacteria (Desulfovibrio desulfuricans ND132) can also methylate elemental mercury5. The prevalence of this trait among different bacterial strains and species remains unclear, however. Here, we compare the ability of two strains of the sulphate-reducing bacterium Desulfovibrio and one strain of the iron-reducing bacterium Geobacter to oxidise and methylate elemental mercury in a series of laboratory incubations. Experiments were carried out under dark, anaerobic conditions, in the presence of environmentally-relevant concentrations of elemental mercury. We report differences in the ability of these organisms to oxidise and methylate elemental mercury. In line with recent findings5, we show that Desulfovibrio desulfuricans ND132 can both oxidise and methylate elemental mercury. However, the rate of methylation of elemental mercury is only about one third the rate of methylation of oxidized mercury. We also show that Desulfovibrio alaskensis G20 can oxidise, but not methylate, elemental mercury. Geobacter sulfurreducens PCA is able to oxidise and methylate elemental mercury in the presence of cysteine. We suggest that the activity of methylating and non-methylating bacteria may together enhance the formation of methylmercury in anaerobic environments.

  16. Degradation of Staphylococcus aureus bacteria by neutral oxygen atoms

    SciTech Connect (OSTI)

    Cvelbar, U.; Mozetic, M. [Jozef Stefan Institute, Jamova cesta 39, SI-1000 Ljubljana (Slovenia); Hauptman, N.; Klanjsek-Gunde, M. [National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana (Slovenia)

    2009-11-15T23:59:59.000Z

    The degradation of Staphylococcus aureus bacteria during treatment with neutral oxygen atoms was monitored by scanning electron microscopy. Experiments were performed in an afterglow chamber made from borosilicate glass. The source of oxygen atoms was remote inductively coupled radiofrequency oxygen plasma. The density of atoms at the samples was 8x10{sup 20} m{sup -3}. The treatment was performed at room temperature. The first effect was the removal of dried capsule. Capsule on exposed parts of bacteria was removed after receiving the dose of 6x10{sup 23} at./m{sup 2}, while the parts of capsule filling the gaps between bacteria were removed after receiving the dose of 2.4x10{sup 24} m{sup -2}. After removing the capsule, degradation continued as etching of bacterial cell wall. The etching was rather nonuniform as holes with diameter of several 10 nm were observed. The cell wall was removed after receiving the dose of about 7x10{sup 24} m{sup -2}. The etching probabilities were about 2x10{sup -5} for the capsule and 2x10{sup -6} for the cell wall. The results were explained by different compositions of capsule and the cell wall.

  17. UNDERSTANDING QUALITY OF LIFE IN ASSISTED LIVING

    E-Print Network [OSTI]

    UNDERSTANDING QUALITY OF LIFE IN ASSISTED LIVING: INFLUENCE OF THE BUILT AND ORGANIZATIONAL- institutional environment who do not need 24/7 nursing care Focus on quality of life (QoL) 194 registered ALs 6.2 % % Chinese 35.1 51.8 55.6 % South Asian 24.5 26.1 23.5 % Filipino 7.1 5.6 5.7 Total 66.7 83.5 84.9 #12;Our

  18. Fission barriers and half-lives

    SciTech Connect (OSTI)

    Moeller, P.; Nix, J.R.; Swiatecki, W.J.

    1989-01-01T23:59:59.000Z

    We briefly review the development of theoretical models for the calculation of fission barriers and half-lives. We focus on how results of actual calculations in a unified macroscopic-microscopic approach provide an interpretation of the mechanisms behind some of the large number of phenomena observed in fission. As instructive examples we choose studies of the rapidly varying fission properties of elements at the end of the periodic system. 31 refs., 10 figs.

  19. Engineering Bacteria for Efficient Fuel Production: Novel Biological Conversion of Hydrogen and Carbon Dioxide Directly into Free Fatty Acids

    SciTech Connect (OSTI)

    None

    2010-07-12T23:59:59.000Z

    Electrofuels Project: OPX Biotechnologies is engineering a microorganism currently used in industrial biotechnology to directly produce a liquid fuel from hydrogen and carbon dioxide (CO2). The microorganism has the natural ability to use hydrogen and CO2 for growth. OPX Biotechnologies is modifying the microorganism to divert energy and carbon away from growth and towards the production of liquid fuels in larger, commercially viable quantities. The microbial system will produce a fuel precursor that can be chemically upgraded to various hydrocarbon fuels.

  20. Trapping and Assembly of Living Colloids at Water/Water Interfaces

    E-Print Network [OSTI]

    Sarah D. Hann; Mark Goulian; Daeyeon Lee; Kathleen J. Stebe

    2014-12-11T23:59:59.000Z

    We study the assembly of colloids in a two phase water-water system that provides an environment that can sustain bacteria, providing a new structure with rich potential to confine and structure living colloids. The water-water system, formed via phase separation of a casein and xanthan mixture, forms a 3-D structure of coexisting casein-rich and xanthan-rich phases. Fluorescent labelling and confocal microscopy reveal the attachment of these living colloids, including Escherichia coli and Pseudomonas aeruginosa, at the interface between the two phases. Inert colloids also become trapped at the interfaces, suggesting that the observed attachment can be attributed to capillarity. Over time, these structures coarsen and eventually degrade, illustrating the dynamic nature of these systems. This system lays the foundation for future studies of the interplay of physicochemical properties of the fluid interfaces and bulk phases and microbial responses they provoke to induce complex spatial organization, to study species which occupy distinct niches, and to optimize efficient microbial cross-feeding or protection from competitors.

  1. Solar Decathlon Team Leading the Way Toward Sustainable Living...

    Office of Environmental Management (EM)

    Team Leading the Way Toward Sustainable Living, Even in the Wake of Disasters Solar Decathlon Team Leading the Way Toward Sustainable Living, Even in the Wake of Disasters August...

  2. Intelligent Transportation Systems: Saving Lives, Time and Money Kristin Tufte

    E-Print Network [OSTI]

    Bertini, Robert L.

    Intelligent Transportation Systems: Saving Lives, Time and Money Kristin Tufte Portland State University Oregon Transportation Summit Sept 10, 2010 #12;Intelligent Transportation Systems: Saving Lives, Time and Money Official transportation data archive for the Portland-Vancouver metropolitan region

  3. Search for Short Lived Particles in High Multiplicity Environment

    E-Print Network [OSTI]

    Marek Gazdzicki; Waldemar Retyk; Jan Pluta

    1999-04-14T23:59:59.000Z

    A method of statistical selection of short lived particles in high multiplicity nucleus-nucleus collisions is discussed.

  4. AN EARLY WARNING SYSTEM FOR AMBIENT ASSISTED LIVING

    E-Print Network [OSTI]

    Karlsson, Brynjar

    AN EARLY WARNING SYSTEM FOR AMBIENT ASSISTED LIVING June 2012 Andrea Monacchi Master of Science in Computer Science #12;#12;AN EARLY WARNING SYSTEM FOR AMBIENT ASSISTED LIVING Andrea Monacchi Master ISSN 1670-8539 #12;#12;An Early Warning System for Ambient Assisted Living by Andrea Monacchi Project

  5. Software Product Line Engineering for Long-lived, Sustainable Systems

    E-Print Network [OSTI]

    Lutz, Robyn R.

    Software Product Line Engineering for Long-lived, Sustainable Systems Robyn Lutz1,2, David Weiss1 system, long-lived system, variability, commonality/variability analysis. 1 Introduction Sustainable and requirements, distinguishes sustainable systems from legacy systems. Accordingly we use the term long-lived

  6. Student Accommodation and Living Guide 2012/2013

    E-Print Network [OSTI]

    Zharkova, Valentina V.

    to sustainable living that has won our campus the award for `Outstanding Contribution to Sustainable DevelopmentStudent Accommodation and Living Guide 2012/2013 #12;For Further Information: Tel: (0)1274 234883 STUDENT ACCOMMODATION AND LIVING GUIDE 2012-2013 City Campus 2 The City of Bradford 4 City Campus Map 6

  7. SEMINOLES CITYINTHE YOUR GUIDE TO TALLAHASSEE & OFF-CAMPUS LIVING

    E-Print Network [OSTI]

    McQuade, D. Tyler

    roommate and neighbor relationships, and develop a sustainable style of living while in TallahasseeSEMINOLES CITYINTHE YOUR GUIDE TO TALLAHASSEE & OFF-CAMPUS LIVING 2013 #12;#12;Seminole Creed and living in the University and beyond. Freedom of Speech and Inquiry I will support academic freedom

  8. Intelligent Transportation Systems: Saving Lives, Time and Money Kristin Tufte

    E-Print Network [OSTI]

    Bertini, Robert L.

    Intelligent Transportation Systems: Saving Lives, Time and Money Kristin Tufte Portland State University June 23, 2010 #12;Intelligent Transportation Systems: Saving Lives, Time and Money 1,400,000 urban cities 3 counties 1 region #12;Intelligent Transportation Systems: Saving Lives, Time and Money Why Now

  9. The secret lives of polynomial identities Bruce Reznick

    E-Print Network [OSTI]

    Reznick, Bruce

    The secret lives of polynomial identities Bruce Reznick University of Illinois at Urbana-Champaign The secret lives of polynomial identities #12;"An idea which can be used only once is a trick. If you can use of Illinois at Urbana-Champaign The secret lives of polynomial identities #12;"An idea which can be used only

  10. The secret lives of polynomial identities Bruce Reznick

    E-Print Network [OSTI]

    Reznick, Bruce

    The secret lives of polynomial identities Bruce Reznick University of Illinois at Urbana, 2010 Math 499, March 2, 2011 Bruce Reznick University of Illinois at Urbana-Champaign The secret lives-Champaign The secret lives of polynomial identities #12;"An idea which can be used only once is a trick. If you can use

  11. Proton radioactivity half lives with Skyrme interactions

    E-Print Network [OSTI]

    T. R. Routray; Abhishek Mishra; S. K. Tripathy; B. Behera; D. N. Basu

    2012-05-31T23:59:59.000Z

    The potential barrier impeding the spontaneous emission of protons in the proton radioactive nuclei is calculated as the sum of nuclear, Coulomb and centrifugal contributions. The nuclear part of the proton-nucleus interaction potential is obtained in the energy density formalism using Skyrme effective interaction that results into a simple algebraic expression. The half-lives of the proton emitters are calculated for the different Skyrme sets within the improved WKB framework. The results are found to be in reasonable agreement with the earlier results obtained for more complicated calculations involving finite range interactions.

  12. ARM - General Changes in Daily Lives

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006Datastreamstwrcam40m DocumentationJanuary 9, 2009 [Events, FeatureListGeneral Changes in Daily Lives Outreach

  13. Live pathogens: rapid detection technique developed

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC)Integrated Codes |Is Your HomeLatestCenter (LMI-EFRC) -ChoicesListLive Discussion

  14. Community is Live!! | OpenEI Community

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual Siteof EnergyInnovationin UrbanCityCoatedCommunity ElectricPreliminaryCommunity is Live!!

  15. Rural Living Canada Website | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f < RAPID‎ |Rippey Jump to:WY) Jump to:Rosneft JumpRugbyLiving Canada

  16. Living Walls | OpenEI Community

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual SiteofEvaluatingGroup |Jilin ZhongdiantouLichuan CityLiqcrytech LLCLiuzhou MinghuiLiving Walls

  17. Detergent composition comprising a cellulase containing cell-free fermentate produced from microorganism ATCC 55702 or mutant thereof

    DOE Patents [OSTI]

    Dees, H.C.

    1998-07-14T23:59:59.000Z

    Bacteria which produce large amounts of a cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  18. Detergent composition comprising a cellulase containing cell-free fermentate produced from microorganism ATCC 55702 or mutant thereof

    DOE Patents [OSTI]

    Dees, H. Craig (Lenoir City, TN)

    1998-01-01T23:59:59.000Z

    Bacteria which produce large amounts of a cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  19. Process for generation of hydrogen gas from various feedstocks using thermophilic bacteria

    DOE Patents [OSTI]

    Ooteghem, Suellen Van (Morgantown, WV)

    2005-09-13T23:59:59.000Z

    A method for producing hydrogen gas is provided comprising selecting a bacteria from the Order Thermotogales, subjecting the bacteria to a feedstock and to a suitable growth environment having an oxygen concentration below the oxygen concentration of water in equilibrium with air; and maintaining the environment at a predetermined pH and at a temperature of at least approximately 45.degree. C. for a time sufficient to allow the bacteria to metabolize the feedstock.

  20. Process for Generation of Hydrogen Gas from Various Feedstocks Using Thermophilic Bacteria

    SciTech Connect (OSTI)

    Ooteghem Van, Suellen

    2005-09-13T23:59:59.000Z

    A method for producing hydrogen gas is provided comprising selecting a bacteria from the Order Thermotogales, subjecting the bacteria to a feedstock and to a suitable growth environment having an oxygen concentration below the oxygen concentration of water in equilibrium with air; and maintaining the environment at a predetermined pH and at a temperature of at least approximately 45 degrees C. for a time sufficient to allow the bacteria to metabolize the feedstock.

  1. antibody-positive women living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Kenong Wu; David Gauthier; Martin D. Levine 1993-01-01 128 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  2. Purple Bacteria Develops Its Own Form of Sunscreen | U.S. DOE...

    Office of Science (SC) Website

    Yellowstone National Park, is due to carotenoid molecules, produced by huge mats of algae and bacteria (Photo from http:en.wikipedia.orgwikiCarotenoid External link )...

  3. anaerobic sulfate-reducing bacteria: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Diversity and characterization of sulfate-reducing bacteria in groundwater at a uranium mill tailings site CiteSeer Summary: plays a role in both natural attenuation and...

  4. Living anionic polymerization using a microfluidic reactor

    SciTech Connect (OSTI)

    Iida, Kazunori; Chastek, Thomas Q.; Beers, Kathryn L.; Cavicchi, Kevin A.; Chun, Jaehun; Fasolka, Michael J.

    2009-02-01T23:59:59.000Z

    Living anionic polymerizations were conducted within aluminum-polyimide microfluidic devices. Polymerizations of styrene in cyclohexane were carried out at various conditions, including elevated temperature (60 °C) and high monomer concentration (42%, by volume). The reactions were safely maintained at a controlled temperature at all points in the reactor. Conducting these reactions in a batch reactor results in uncontrolled heat generation with potentially dangerous rises in pressure. Moreover, the microfluidic nature of these devices allows for flexible 2D designing of the flow channel. Four flow designs were examined (straight, periodically pinched, obtuse zigzag, and acute zigzag channels). The ability to use the channel pattern to increase the level of mixing throughout the reactor was evaluated. When moderately high molecular mass polymers with increased viscosity were made, the patterned channels produced polymers with narrower PDI, indicating that passive mixing arising from the channel design is improving the reaction conditions.

  5. Living and Working in the Freezer

    SciTech Connect (OSTI)

    Hill, Victoria (Dept of Ocean, Earth, and Atmospheric Sciences, Old Dominion Unversity) [Dept of Ocean, Earth, and Atmospheric Sciences, Old Dominion Unversity

    2012-02-07T23:59:59.000Z

    Very little data of any kind exists from the early spring in the Arctic. The reason? It's extremely cold and that makes it difficult to survive, let alone conduct science. From March through the end of April, 2011, scientists from around the world braved temperatures of -48?C in the high Canadian Arctic in the name of science. At the Catlin Arctic Survey's floating 'Ice Base' off Ellef Ringnes Island, Dr. Victoria Hill was investigating how organic material in fresh water near the surface of the ocean may be trapping heat from the sun, causing the upper ocean layers to warm. This is a very new area of research and this mechanism represents a key uncertainty in accurate modeling of ice thickness and upper ocean heat content. In this presentation Dr. Hill will talk about living and working at the ice base and discuss preliminary data from the expedition.

  6. RESEARCH Open Access Overexpression of an exotic thermotolerant

    E-Print Network [OSTI]

    Qin, Wensheng

    such as bioethanol. Different microorganisms such as fungi and bacteria primarily initiate the biocon- version

  7. Slow urban living apartments : transformation of five story walk-up apartments in Seoul

    E-Print Network [OSTI]

    Lim, Yihyun

    2014-01-01T23:59:59.000Z

    Experiential living is the new trend for future living. Whether it is through living in micro spaces, flexible units, mixed-use developments, practicing urban farming, or sharing lifestyles, these different trends of living ...

  8. Fe-CYCLE BACTERIA FROM INDUSTRIAL CLAYS MINED IN GEORGIA, USA EVGENYA S. SHELOBOLINA

    E-Print Network [OSTI]

    Lovley, Derek

    Fe-CYCLE BACTERIA FROM INDUSTRIAL CLAYS MINED IN GEORGIA, USA EVGENYA S. SHELOBOLINA 1, *,{, SAM M are major discoloring impurities in mined commercial white kaolin clay. In order to evaluate the potential influence of Fe-cycle bacteria on Fe cycling during post- depositional clay-weathering alteration, Fe

  9. Clay enhancement of methane, low molecular weight hydrocarbon and halocarbon conversion by methanotrophic bacteria

    DOE Patents [OSTI]

    Apel, William A.; Dugan, Patrick R.

    1995-04-04T23:59:59.000Z

    An apparatus and method for increasing the rate of oxidation of toxic vapors by methanotrophic bacteria. The toxic vapors of interest are methane and trichloroethylene. The apparatus includes a gas phase bioreactor within a closed loop pumping system or a single pass system. The methanotrophic bacteria include Methylomonas methanica, Methylosinus trichosporium, and uncharacterized environmental enrichments.

  10. Clay enhancement of methane, low molecular weight hydrocarbon and halocarbon conversion by methanotrophic bacteria

    DOE Patents [OSTI]

    Apel, William A. (Idaho Falls, ID); Dugan, Patrick R. (Idaho Falls, ID)

    1995-01-01T23:59:59.000Z

    An apparatus and method for increasing the rate of oxidation of toxic vapors by methanotrophic bacteria. The toxic vapors of interest are methane and trichloroethylene. The apparatus includes a gas phase bioreactor within a closed loop pumping system or a single pass system. The methanotrophic bacteria include Methylomonas methanica, Methylosinus trichosporium, and uncharacterized environmental enrichments.

  11. Impact of carbon nanotubes on the ingestion and digestion of bacteria by

    E-Print Network [OSTI]

    Le Roy, Robert J.

    Impact of carbon nanotubes on the ingestion and digestion of bacteria by ciliated protozoa PARNIAN,4 by ecotoxicologists because of its role in the regulation of microbial populations through the ingestion and digestion to aggregate, which impedes their ability to ingest and digest their prey bacteria species, although it might

  12. What is the Role of Arsenite-Oxidising Bacteria in Dissolving Arsenic Minerals?

    E-Print Network [OSTI]

    Crawford, Ian

    What is the Role of Arsenite-Oxidising Bacteria in Dissolving Arsenic Minerals? Supervisors: Dr through dissolution of arsenic-bearing minerals. This process is mediated by bacteria, which can break down the mineral lattice by extracting nutrient elements such as potassium, or by causing redox changes

  13. Novel Methane, Ethane, and Propane Oxidizing Bacteria at Marine Hydrocarbon Seeps Identified by Stable Isotope Probing

    E-Print Network [OSTI]

    Sessions, Alex L.

    Novel Methane, Ethane, and Propane Oxidizing Bacteria at Marine Hydrocarbon Seeps Identified by Stable Isotope Probing Running Title: Novel Methane, Ethane, and Propane Oxidizing Bacteria Section incubating sediment with 13 C-labeled methane, ethane, or propane, we5 confirmed the incorporation of 13 C

  14. Design, testing and optimization of a microfluidic device for capture and concentration of bacteria 

    E-Print Network [OSTI]

    Cherla, Srinivas

    2006-10-30T23:59:59.000Z

    ¢Â?Â?Critical Design ParametersâÂ?Â? and âÂ?Â?Design ConstraintsâÂ?Â?. Electrokinetic phenomena are used to exploit the surface charges on bacteria. Electrophoresis is used to transport the bacteria to electrode surface and âÂ?Â?Electrostatic trapping...

  15. Algal Research1 Screening and selection of growth-promoting bacteria for Dunaliella cultures2

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    and potential applications for microalgae, which include food,44 animal feed, healthcare, energy microalgae species and identified using 16S rRNA phylogenetic analysis. The recovered bacteria were27 found38 accumulation in Dunaliella sp. cells.39 Keywords: Microalgae, Dunaliella, bacteria, interaction

  16. Microbial metaproteomics: Identifying the repertoire of proteins that microorganisms use to compete and cooperate in complex environmental communities

    SciTech Connect (OSTI)

    Hettich, Robert {Bob} L [ORNL; Sharma, Ritin [ORNL; Chourey, Karuna [ORNL; Giannone, Richard J [ORNL

    2012-01-01T23:59:59.000Z

    The availability of genome information for microbial consortia, including unculturable species, from environmental samples has enabled systems-biology interrogation by providing a means to access genomic, transcriptomic, and proteomic information. This provides a unique opportunity to characterize the molecular activities and interactions of these microbial systems at a comprehensive level never before possible. Such information not only provides details about the organizational, functional, and metabolic activities of such systems, but also the untapped reserve of molecular activities that might be invoked and exploited under certain environmental conditions. Since bacteria naturally exist in complex ecosystems, it is imperative to develop and utilize analytical approaches that can provide molecular level details on systems consisting of mixed microbial membership. This is the realm of metaproteomics - the characterization of the complement of proteins expressed by a microbial community in an environmental sample

  17. Sandia National Laboratories: ensure we have a living river

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ensure we have a living river Sandia, the Atlantic Council, and NM Water Resource Research Institute Sponsor Roundtable on Western Water Scarcity On October 4, 2013, in Climate,...

  18. aboriginal children living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    resources appropriately 12;Aboriginal people - the first environmentalists Truly sustainable living (Zero work with the Nyungar community to not only continue, but grow...

  19. assisted living facilities: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    related field o have experience working in an educational setting o Sheridan, Jennifer 15 Sustainable Living Facilities (SLF) Requirements, Expectations, Pledge and Agreement...

  20. asian communities living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Colleges within large Universities Barrash, Warren 7 University Housing Designing Sustainable Communities for Living and Learning Biology and Medicine Websites Summary: The...

  1. Brownsville Public Utilities Board- Green Living Residential Rebate Program

    Broader source: Energy.gov [DOE]

    Brownsville Public Utilities Board offers residential customers rebates for installation of energy efficient measures. Through the Green Living Rebate program, customers can apply for rebates for...

  2. Dutch Company Powers Streetlights With Living Plants; Will Your...

    Open Energy Info (EERE)

    Dutch Company Powers Streetlights With Living Plants; Will Your Cell Phone Be Next? Home > Groups > OpenEI Community Central Dc's picture Submitted by Dc(266) Contributor 16...

  3. Mass Spectral Molecular Networking of Living Microbial Colonies...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Living Microbial Colonies. Abstract: Integrating the governing chemistry with the genomics and phenotypes of microbial colonies has been a "holy grail" in...

  4. Spontaneous fission half-lives for ground state nuclides

    SciTech Connect (OSTI)

    Holden, N.E. [Brookhaven National Lab., Upton, NY (United States); Hoffman, D.C. [Lawrence Berkeley Lab., CA (United States)

    1993-08-01T23:59:59.000Z

    Measurements of the spontaneous fission half-lives of nuclides of elements Z = 90 to 108 have been compiled and evaluated. Recommended values are presented.

  5. Final technical report for project titled Quantitative Characterization of Cell Aggregation/Adhesion as Predictor for Distribution and Transport of Microorganisms in Subsurface Environment

    SciTech Connect (OSTI)

    Gu, April Z [Northeastern University; Wan, Kai-tak [Northeastern Univeristy

    2014-09-02T23:59:59.000Z

    This project aims to explore and develop enabling methodology and techniques for nano-scale characterization of microbe cell surface contact mechanics, interactions and adhesion quantities that allow for identification and quantification of indicative properties related to microorganism migration and transport behavior in porous media and in subsurface environments. Microbe transport has wide impact and therefore is of great interest in various environmental applications such as in situ or enhanced subsurface bioremediation,filtration processes for water and wastewater treatments and protection of drinking water supplies. Although great progress has been made towards understanding the identities and activities of these microorganisms in the subsurface, to date, little is known of the mechanisms that govern the mobility and transport of microorganisms in DOE’s contaminated sites, making the outcomes of in situ natural attenuation or contaminant stability enhancement unpredictable. Conventionally, movement of microorganisms was believed to follows the rules governing solute (particle) transport. However, recent studies revealed that cell surface properties, especially those pertaining to cell attachment/adhesion and aggregation behavior, can cause the microbe behavior to deviate from non-viable particles and hence greatly influence the mobility and distribution of microorganisms in porous media.This complexity highlights the need to obtain detailed information of cell-cell and cell-surface interactions in order to improve and refine the conceptual and quantitative model development for fate and transport of microorganisms and contaminant in subsurface. Traditional cell surface characterization methods are not sufficient to fully predict the deposition rates and transport behaviors of microorganism observed. A breakthrough of methodology that would allow for quantitative and molecular-level description of intrinsic cell surface properties indicative for cell-surface interactions is essential for the field. To tackle this, we have developed a number of new Bio-nanomechanical techniques, including reflection interference contrast microscopy (RICM) and bio-AFM (Atomic Force Microscopy), for cell adhesion-detachment measurement of the long-range surface interactions, in combination with mathematical modeling, which would allow us to characterize the mechanical behavior from single cell to multi-cell aggregate, critical thresholds for large scale coaggregation and transportation of cells and aggregates in the presence of long range inter-surface forces etc. Although some technical and mathematical challenges remain, the preliminary results promise great breakthrough potential. In this study, we investigated the cellular surface characteristics of representative bio-remediating microorganisms relevant to DOE IFRC (Integrated Field-Scale Subsurface Research Challenges) sites and their transport behaviors in porous media, aiming to draw a groundbreaking correlation between the micro-scale genetic and biological origin-based cell surface properties, the consequent mechanical adhesion and aggregation behaviors, and the macro-scale microbial mobility and retention in porous media, which are unavailable in the literature. The long-term goal is to significantly improve the mechanistic and quantitative understanding of microbial mobility, sorption, and transport within reactive transport models as needed to manipulate subsurface contaminant fate and transport predictions.

  6. Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

    DOE Patents [OSTI]

    Bavykin, Sergei G. (Darien, IL); Mirzabekova, legal representative, Natalia V. (Westmont, IL); Mirzabekov, deceased, Andrei D. (Westmont, IL)

    2007-12-04T23:59:59.000Z

    The present invention relates to methods and compositions for using nucleotide sequence variations of 16S and 23S rRNA within the B. cereus group to discriminate a highly infectious bacterium B. anthracis from closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations and discriminate B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed samples, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

  7. Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

    DOE Patents [OSTI]

    Bavykin, Sergei G. (Darien, IL); Mirzabekov, Andrei D. (Moscow, RU)

    2007-10-30T23:59:59.000Z

    The present invention is directed to a novel method of discriminating a highly infectious bacterium Bacillus anthracis from a group of closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations. The identification and analysis of these sequence variations enables positive discrimination of isolates of the B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed probes, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

  8. This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research

    E-Print Network [OSTI]

    Coley, Phyllis

    and endophyte were collected. The extract was inactive to the human parasites Trypanosoma cruzi, Leishmania reserved. Introduction Endophytes are microorganisms including bacteria or fungi that live within,2 Endophytic microbes have been recognized as important sources of structurally novel and biologically active

  9. Why we live in 3 Dimensions

    E-Print Network [OSTI]

    Carlos Castro; Alex Granik; M. S. El Naschie

    2000-08-18T23:59:59.000Z

    A Cantorian fractal spacetime, a family member of von Neumann's noncommutative geometry is introduced as a geometry underlying a new relativity theory which is similar to the relation between general relativity and Riemannian geometry. Based on this model and the new relativity theory an ensemble distribution of all the dimensions of quantum spacetime is derived with the help of Fermat grand theorem. The calculated average dimension is very close to the value of $4+\\phi^3 $ (where $\\phi$ is the golden mean) obtained by El Naschie on the basis of a different approach. It is shown that within the framework of the new relativity the cosmological constant problem is nonexistent, since the Universe self-organizes and self-tunes according to the renormalization group (RG) flow with respect to a local scaling microscopic arrow of time. This implies that the world emerged as a result of a non-equilibrium process of self-organized critical phenomena launched by vacuum fluctuations in Cantorian fractal spacetime $\\cal E^{\\infty}$. It is shown that we are living in a metastable vacuum and are moving towards a fixed point ($ D$ = 4+$\\phi^3$) of the RG. After reaching this point, a new phase transition will drive the universe to a quasi-crystal phase of the lower average dimension of $\\phi^3$.

  10. Radiation-resistant microorganism

    DOE Patents [OSTI]

    Fliermans, Carl B.

    2010-06-15T23:59:59.000Z

    An isolated and purified bacterium is provided which was isolated from a high-level radioactive waste site of mixed waste. The isolate has the ability to degrade a wide variety of organic contaminants while demonstrating high tolerance to ionizing radiation. The organism is uniquely suited to bioremediation of a variety or organic contaminants while in the presence of ionizing radiation.

  11. Genetically modified microorganisms for

    E-Print Network [OSTI]

    Angenent, Lars T.

    -proteins. Thus far, Shewanella oneidensis and Geobacter sulfurreducens have been extensively studied into electricity in much more efficient ways. This chapter will discuss recent work on the investigation be possible in the near future. 6.2 EXTRACELLULAR RESPIRATION IN SHEWANELLA ONEIDENSIS AND GEOBACTER

  12. Radiation-resistant microorganism

    DOE Patents [OSTI]

    Fliermans, Carl B.

    2007-01-09T23:59:59.000Z

    An isolated and purified bacterium is provided which was isolated from a high-level radioactive waste site of mixed waste. The isolate has the ability to degrade a wide variety of organic contaminants while demonstrating high tolerance to ionizing radiation. The organism is uniquely suited to bioremediation of a variety or organic contaminants while in the presence of ionizing radiation.

  13. Effect of Collection Method and Archiving Conditions on the Survivability of Vegetative and Spore Forming Bacteria

    E-Print Network [OSTI]

    Kassab, Asmaa S.

    2010-10-12T23:59:59.000Z

    and Schafer, 1998). Aerosols play a large role in a multiplicity of different production processes. Airborne particles, especially pathogenic microorganisms and other biological materials, are potential public and industrial health hazards. To ensure... the effective protection of This thesis followed the style and format of Aerosol Science and Technology. 2 both general and occupational populations from the adverse effects of airborne hazards, reliable...

  14. Dynamic estimation of specific growth rates and concentrations of bacteria for the anaerobic digestion

    E-Print Network [OSTI]

    Boyer, Edmond

    measured quantities ­ the dilution rate and the flow rates of methane and carbon dioxide in the biogas by microorganisms into biogas (methane and carbon dioxide) and digestate (natural manure) in the absence of oxygen [1, 2, 6]. The biogas is an additional energy source and the methane is a greenhouse gas

  15. housing.ucdavis.edu Living at UC Davis

    E-Print Network [OSTI]

    Hernes, Peter J.

    housing.ucdavis.edu Living at UC Davis Apartments Living at UC Davis STUDENT HOUSING #12;2 Every year, Student Housing is home to over 6000 UC Davis students, over 500 of whom are transfer students and students participating in graduate and professional programs. UC Davis also provides housing that is ideal

  16. an interview with Richard Alba Extraordinary Lives: Patti Smith

    E-Print Network [OSTI]

    Dennehy, John

    When racial boundaries fade an interview with Richard Alba Extraordinary Lives: Patti Smith What on the workforce, the family, and American society. EXTRAORDINARY LIVES: PATTI SMITH 12 This was the first featured the maximally talented Patti Smith--singer, songwriter, artist and poet--who spoke thoughtfully

  17. Yugoslavia is DeaD long live the Yugosphere

    E-Print Network [OSTI]

    Neirotti, Juan Pablo

    Tim Judah Yugoslavia is DeaD long live the Yugosphere tim JuDah Good news from the Western Balkans JuDah Good news from the Western Balkans #12;Yugoslavia is Dead . . . Long Live the Yugosphere LSEE happened. In that case how can they react properly? How can we correctly analyse the Western Balkans

  18. Transportation Agency Tool to Analyze Benefits of Living Snow Fences

    E-Print Network [OSTI]

    Minnesota, University of

    Transportation Agency Tool to Analyze Benefits of Living Snow Fences 5/31/12 Transportation Agency/31/12 Transportation Agency Tool to Analyze Benefits of Living Snow Fences Center for Integrated Natural Resources, Mobility, & Transportation Authority Benefits, Farmer Costs, & Carbon Impacts Focus Groups and Surveys

  19. Carbon nanotubes as photoacoustic molecular imaging agents in living mice

    E-Print Network [OSTI]

    Khuri-Yakub, Butrus T. "Pierre"

    Carbon nanotubes as photoacoustic molecular imaging agents in living mice ADAM DE LA ZERDA1 not shown to target a diseased site in living subjects. Here we show that single-walled carbon nanotubes were verified ex vivo using Raman microscopy. Photoacoustic imaging of targeted single-walled carbon

  20. "Space Station" Theme: Learning to Work, and Live, in Space

    E-Print Network [OSTI]

    Mathis, Wayne N.

    "Space Station" IMAX Film Theme: Learning to Work, and Live, in Space The educational value of NASM visit and afterward. See the "Alignment with Standards" table for details regarding how "Space Station in the "Space Station" program: · How astronauts train · What it is like to live and work in Space aboard

  1. The secret lives of polynomial identities Bruce Reznick

    E-Print Network [OSTI]

    Reznick, Bruce

    The secret lives of polynomial identities Bruce Reznick University of Illinois at Urbana-Champaign University of Washington May 18, 2012 Bruce Reznick University of Illinois at Urbana-Champaign The secret of Illinois at Urbana-Champaign The secret lives of polynomial identities #12;"An idea which can be used only

  2. The secret lives of polynomial identities Bruce Reznick

    E-Print Network [OSTI]

    Reznick, Bruce

    The secret lives of polynomial identities Bruce Reznick University of Illinois at Urbana-Champaign University of Konstanz June 30, 2011 Bruce Reznick University of Illinois at Urbana-Champaign The secret of Illinois at Urbana-Champaign The secret lives of polynomial identities #12;"An idea which can be used only

  3. basic student budget 2014 |2015 LIVING ON CAMPUS

    E-Print Network [OSTI]

    Painter, Kevin

    basic student budget 2014 |2015 #12;LIVING ON CAMPUS HALL FEES £ 119.18 ­ average cost x 40 weeks at £119.18 per week, and may be cheaper depending on type of accommodation. This budget is meant months (whether you live in the flat over the summer or not). This budget is meant as a realistic guide

  4. Genome-Assisted Analysis of Dissimilatory Metal-Reducing Bacteria

    SciTech Connect (OSTI)

    Fredrickson, Jim K.; Romine, Margaret F.

    2005-06-01T23:59:59.000Z

    Whole genome sequence for Shewanella oneidensis and Geobacter sulfurreducens has provided numerous new biological insights into the function of these model dissimilatory metal-reducing bacteria. Many of the discoveries, including the identification of a high number of c-type cytochromes in both organisms, have been the result of comparative genomic analyses including several that were experimentally confirmed. Genome sequence has also aided the identification of genes important for the reduction of metal ions and other electron acceptors utilized by these organisms during anaerobic growth by facilitating the identification of genes disrupted by random insertions. Technologies for assaying global expression patterns for genes (mRNA) and proteins have also been enabled by the availability of genome sequence but their application has been limited mainly to the analysis of the role of global regulatory genes and to identifying genes expressed or repressed in response to specific electron acceptors. It is anticipated that details regarding the mechanisms of metal ion respiration, and metabolism in general, will eventually be revealed by comprehensive, systems-level analyses enabled by functional genomic analyses.

  5. Howell, R.A., 2012 Living with a carbon allowance 1 Living with a carbon allowance: the experiences of Carbon

    E-Print Network [OSTI]

    Howell, R.A., 2012 Living with a carbon allowance 1 Living with a carbon allowance: the experiences of Carbon Rationing Action Groups and implications for policy Rachel A. Howell Environmental Change with a carbon allowance: the experiences of Carbon Rationing Action Groups and implications for policy. Energy

  6. Think big! Bacteria breach cell division size limit posted by news on september 15, 2014 -2:30pm

    E-Print Network [OSTI]

    to divide just like the more familiar, but much shorter (~ 2 micrometer), Escherichia coli", states Phd microorganisms thriving on the surface of marine tropical worms are an inexhaustible source of wonder!" exclaims

  7. Short RNA half-lives in the slow-growing marine cyanobacterium Prochlorococcus

    E-Print Network [OSTI]

    Steglich, Claudia

    Background RNA turnover plays an important role in the gene regulation of microorganisms and influences their speed of acclimation to environmental changes. We investigated whole-genome RNA stability of Prochlorococcus, a ...

  8. Short RNA half-lives in the slow-growing marine cyanobacterium Prochlorococcus

    E-Print Network [OSTI]

    Steglich, Claudia

    Background: RNA turnover plays an important role in the gene regulation of microorganisms and influences their speed of acclimation to environmental changes. We investigated whole-genome RNA stability of Prochlorococcus, ...

  9. The battle of bacteria: Agencies, stakeholders focusing on restoring water quality

    E-Print Network [OSTI]

    Foust, Margaret

    2010-01-01T23:59:59.000Z

    to watershed stakeholders, who will determine the next steps in managing water quality in the tributaries. The TMDL task force was also charged with developing a roadmap for scientific research on how bacteria behave under different conditions. Tailored...

  10. Biotemplated Synthesis of Gold NanoparticleBacteria Cellulose Nanofiber Nanocomposites and Their

    E-Print Network [OSTI]

    Qi, Limin

    produced by acetic acid bacteria Acetobacter xylinum using D-glucose as the carbon source, which usually of sisal fibers by in situ fermentation and obtained a new class of completely renewable and biodegradable

  11. Contamination Cross-contamination occurs when bacteria, or "germs," are moved from one food to another.

    E-Print Network [OSTI]

    Combat Cross Contamination Cross-contamination occurs when bacteria, or "germs," are moved from one food to another. To combat cross-contamination, keep foods SEPARATE!! At the grocery store, keep raw

  12. Amoebae/bacteria consortia and uses for degrading wastes and contaminants

    DOE Patents [OSTI]

    Tyndall, Richard L. (Clinton, TN)

    1996-01-01T23:59:59.000Z

    A method of altering trinitrotoluene includes the steps of: providing an amoeba/bacteria consortium, particularly ATCC 40908 or a mutant thereof possessing all the identifying characteristics thereof; and contacting the consortium with trinitrotoluene to alter the trinitrotoluene.

  13. Population Genomics of Early Events in the Ecological Differentiation of Bacteria

    E-Print Network [OSTI]

    Shapiro, B. J.

    Genetic exchange is common among bacteria, but its effect on population diversity during ecological differentiation remains controversial. A fundamental question is whether advantageous mutations lead to selection of clonal ...

  14. Reduction of Antibiotic-Resistant Bacteria Present in Food Animal Manures by Composting and Anaerobic Digestion

    E-Print Network [OSTI]

    Jones, Michelle

    Reduction of Antibiotic-Resistant Bacteria Present in Food Animal Manures by Composting digestion and composting at mesophilic or moderate temperature significantly reduced the antimicrobial resistance in animal manure. The most effective treatment was composting at thermophilic or high temperature

  15. Characterization of Extremophilic Bacteria for Potential in the Biofuel and Bioprocess Industries 

    E-Print Network [OSTI]

    Haynes, Abria R

    2014-04-18T23:59:59.000Z

    of bioprocess byproducts (e.g. lignocellulosic hydrolysate, bio-oil) that are known to harbor both compounds with inhibitory effects on growth and pentose sugars released after hydrolysis. Polyhydroxyalkanoates (PHA) are bio-plastics produced by some bacteria...

  16. Effects of UV Light Disinfection on Tetracycline Resistant Bacteria in Wastewater Effluents 

    E-Print Network [OSTI]

    Childress, Hannah

    2011-10-21T23:59:59.000Z

    of antibioticresistance genes (ARGs) and antibiotic-resistant and multidrug resistant bacteria in wastewater and drinking water treatment plants. There is also evidence to suggest that ARGs spread to the environment, and to humans and animals, through wastewater effluents...

  17. Engineering Bacteria for Production of Rhamnolipid as an Agent for Enhanced

    E-Print Network [OSTI]

    Goddard III, William A.

    ARTICLE Engineering Bacteria for Production of Rhamnolipid as an Agent for Enhanced Oil Recovery applications, including enhanced oil recovery (EOR), biodegradation, and bio- remediation. Rhamnolipid; transposome; chromosomal insertion; interfacial tension; enhanced oil recovery Introduction Surfactants

  18. Study of Midgut Bacteria in the Red Imported Fire Ant, Solenopsis invicta Büren (Hymenoptera: Formicidae)

    E-Print Network [OSTI]

    Medina, Freder

    2011-08-08T23:59:59.000Z

    that manipulate insect reproduction, development and even provide defense against parasitoids and pathogens. In this study we investigated the presence of bacteria inside the Red Imported Fire Ant midgut using molecular tools and transmission electron microscopy...

  19. Bacteria Harnessing Complexity Eshel Ben Jacob, Yakir Aharonov and Yoash Shapira

    E-Print Network [OSTI]

    Jacob, Eshel Ben

    1 Bacteria Harnessing Complexity By Eshel Ben Jacob, Yakir Aharonov and Yoash Shapira School, exchange of meaning-bearing chemical messages, and dialogues. The meaning-based communication permits

  20. The incidence and significance of anaerobic bacteria in the equine uterus 

    E-Print Network [OSTI]

    Bolinger, Dean Roger

    1987-01-01T23:59:59.000Z

    THE INCIDENCE AND SIGNIFICANCE OF ANAEROBIC BACTERIA IN THE EQUINE UTERUS A Thesis by DEAN ROGER BOLINGER Submitted to the Graduate College of Texas A&M University in partial fulfillment of the requirements of the degree of MASTER OF SCIENCF.... December I 987 Major Subject: Veterinary Medical Sciences THE INCIDENCE AND SIGNIFICANCE OF ANAEROBIC BACTERIA IN THE EQUINE UTERUS A Thesis by DEAN ROGER BOLINGER Approved as to style and content by: Ronnie G. Elmore (Chairman of Committee...

  1. The effect of polyacrylamide polymers and formaldehyde on selected strains of oilfield related bacteria

    SciTech Connect (OSTI)

    Farquhar, G.B. [Texaco E.P.T.D., Houston, TX (United States)

    1996-08-01T23:59:59.000Z

    A preliminary study of the effect of two polyacrylamide polymers and formaldehyde on certain strains of oil field related bacteria was performed. Since at this stage of the development of planning the proposed North Sea polymer flood field samples of native bacteria populations and fluid samples were not available, the preliminary study was made using pure strains of bacteria associated with the oilfield environment. The tests were run using ideal growth conditions of temperature and culture media. The purpose of the study was to determine the effect of proposed polyacrylamide polymer flooding materials on the growth of selected strains of bacteria belonging to families common to the oilfield environment. The effect of 100 ppm of formaldehyde on these organisms also was investigated. The results of the tests showed that these bacteria responded differently to exposure to formaldehyde, polyacrylamide polymers and mixtures of both. These results indicate another possible mechanism for the occurrence of high bacteria related corrosion rates reported to occur on the producing side of polymer floods.

  2. 'Invisible lives' : the Gypsies and Travellers of Britain 

    E-Print Network [OSTI]

    Clark, Colin Robert

    2001-12-05T23:59:59.000Z

    This thesis examines the lives and experiences of relatively 'unknown' minority ethnic communities in Britain. As a group, they have been known by many names since their arrival in the 15th Century, although the most common ...

  3. Oak Leaf Roller and Springtime Defoliation of Live Oak Trees

    E-Print Network [OSTI]

    Drees, Bastiaan M.

    2004-03-26T23:59:59.000Z

    This publication explains how to minimize damage to live oak trees by the oak leaf roller and an associated caterpillar species, which occur throughout Texas but are most damaging in the Hill Country and South Texas....

  4. Our Energy Independence- A Live Chat With Dr. Arun Majumdar

    Broader source: Energy.gov [DOE]

    Join Dr. Arun Majumdar at 2 PM ET for a live, two-way conversation about the investments we're making to build the clean energy infrastructure of the future.

  5. Live Webcast on Recent Wind Energy Technology Advances

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webcast titled “Recent Wind Technology Advances” on April 16, 2014, from 3:00 to 4:00 p.m. Eastern Standard Time.

  6. Live long and prosper: Germline stem cell maintenance

    E-Print Network [OSTI]

    Extavour, Cassandra

    Live long and prosper: Germline stem cell maintenance revisited (retrospective on DOI: 10.1002/bies aging is rapid or their divisions tend to produce game- togenic daughters, then they may limit animals

  7. Spontaneous fission half-lives for ground state nuclides

    SciTech Connect (OSTI)

    Holden, N.E. (Brookhaven National Lab., Upton, NY (United States)); Hoffman, D.C. (Lawrence Berkeley Lab., CA (United States))

    1991-01-01T23:59:59.000Z

    Measurements of the spontaneous fission half-lives of nuclides of elements Z = 90 to 107 have been compiled and evaluated. Recommended values are presented. 126 refs., 96 tabs.

  8. Heavy Long-lived Mossbauer State of Niobium

    E-Print Network [OSTI]

    Cheng, Yao

    2011-01-01T23:59:59.000Z

    A heavy niobium state showing 1/3 residual resistance is discovered below the superconducting transition temperature. This niobium sample contains high-density long-lived Mossbauer excitation.

  9. Heavy Long-lived Mossbauer State of Niobium

    E-Print Network [OSTI]

    Yao Cheng; Chi-Hao Lee

    2011-03-03T23:59:59.000Z

    A heavy niobium state showing 1/3 residual resistance is discovered below the superconducting transition temperature. This niobium sample contains high-density long-lived Mossbauer excitation.

  10. The living commons : a spatial theory for biological design

    E-Print Network [OSTI]

    Telhan, Orkan

    2013-01-01T23:59:59.000Z

    Biological design is as ancient as human civilization. For thousands of years, living systems and natural processes have been manipulated by humans and their biological outcomes have been customized for different purposes. ...

  11. Acting Biomass Program Manager Dr. Valerie Reed to Host Live...

    Broader source: Energy.gov (indexed) [DOE]

    December 16th, the Energy Department (@energy) will be hosting a live Twitter Q&A on biofuels with Dr. Valerie Reed, Acting Manager of the Biomass Program. Dr. Reed holds a Ph. D....

  12. University Housing Designing Sustainable Communities for Living and Learning

    E-Print Network [OSTI]

    Almor, Amit

    The Mission Learning Center for Sustainable Futures Green Learning Community Student Engagement Green Living Public Events Green Education University and Community Outreach Community Building Our Home:Physical Features LEED Certification Sustainable Sites Materials and Resources Energy and Atmosphere Indoor

  13. Development of a Multiplexed, Bead-Based Assessment Tool for Rapid Identification and Quantitation of Microorganisms in Field Samples. Final Report

    SciTech Connect (OSTI)

    Lowe, M.; Halden, R.

    2002-10-09T23:59:59.000Z

    This was the final report for DOE NABIR grant DE-FG02-01ER63264 (PI Mary Lowe). The grant was entitled ''Development of a Multiplexed Bead-Based Assessment Tool for Rapid Identification and Quantitation of Microorganisms in Field Samples.'' The grant duration was one year. The purpose was to develop a bead-based assay for measuring analyte DNAs in environmental PCR products and to apply the method to a field experiment. The primary experiment was located at the UMTRA Old Rifle site.

  14. LiveWall Operational Evaluation: Seattle Law Enforcement Pilot

    SciTech Connect (OSTI)

    Barr, Jonathan L.; Burtner, Edwin R.; Stein, Steven L.

    2013-10-01T23:59:59.000Z

    The LiveWall concept envisioned as an outgrowth of the Precision Information Environment (PIE) project allows communications between separate groups using interactive video, audio, and a shared desktop environment; this allows everyone to participate and collaborate in real time, regardless of location. The LiveWall concept provides a virtual window to other locations, where all parties can interact and collaboratively work with each other. This functionality is intended to improve multi-site coordination amongst emergency operations centers (EOC), field operations sites and across organizations and jurisdictions to accommodate communications during routine and emergency events. For the initial LiveWall operational evaluation PNNL partnered with the Seattle Police Department (SPD). This partnership allowed for the creation of an excellent LiveWall test bed specific to law enforcement. This partnership made it possible to test the LiveWall concept with scenarios involving the many facets of the law enforcement work done by SPD. PNNL and SPD agreed that integrating the systems into operations for a real event would be the best test of the technology and give SPD staff greater visibility into the functionality and benefits offered by the LiveWall concept.

  15. adult-to-adult living donor: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    D. G. Allen; C. R. Stanley; M. S. Sherwin 2005-03-05 92 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  16. L\\'evy Fluctuations and Tracer Diffusion in Dilute Suspensions of Algae and Bacteria

    E-Print Network [OSTI]

    Zaid, Irwin M; Yeomans, Julia M

    2010-01-01T23:59:59.000Z

    Swimming microorganisms rely on effective mixing strategies to achieve efficient nutrient influx. Recent experiments, probing the mixing capability of unicellular biflagellates, revealed that passive tracer particles exhibit anomalous non-Gaussian diffusion when immersed in a dilute suspension of self-motile Chlamydomonas reinhardtii algae. Qualitatively, this observation can be explained by the fact that the algae induce a fluid flow that may occasionally accelerate the colloidal tracers to relatively large velocities. A satisfactory quantitative theory of enhanced mixing in dilute active suspensions, however, is lacking at present. In particular, it is unclear how non-Gaussian signatures in the tracers' position distribution are linked to the self-propulsion mechanism of a microorganism. Here, we develop a systematic theoretical description of anomalous tracer diffusion in active suspensions, based on a simplified tracer-swimmer interaction model that captures the typical distance scaling of a microswimmer'...

  17. Life in the Living Laboratory: An Anthropological Investigation of Environmental Science, Tourism, and Design in the Contemporary Bahamas

    E-Print Network [OSTI]

    Moore, Amelia M.

    2010-01-01T23:59:59.000Z

    envisioned through sustainable designs for living. It can bethe living laboratory- biological, sustainable, ecologicalsustainable economic development- processes of human life and living,

  18. Turning Bacteria into Biofuel: Development of an Integrated Microbial Electrocatalytic (MEC) System for Liquid Biofuel Production from CO2

    SciTech Connect (OSTI)

    None

    2010-08-01T23:59:59.000Z

    Electrofuels Project: LBNL is improving the natural ability of a common soil bacteria called Ralstonia eutropha to use hydrogen and carbon dioxide for biofuel production. First, LBNL is genetically modifying the bacteria to produce biofuel at higher concentrations. Then, LBNL is using renewable electricity obtained from solar, wind, or wave power to produce high amounts of hydrogen in the presence of the bacteria—increasing the organism’s access to its energy source and improving the efficiency of the biofuel-creation process. Finally, LBNL is tethering electrocatalysts to the bacteria’s surface which will further accelerate the rate at which the organism creates biofuel. LBNL is also developing a chemical method to transform the biofuel that the bacteria produce into ready-to-use jet fuel.

  19. Real time imaging of live cell ATP leaking or release events by chemiluminescence microscopy

    SciTech Connect (OSTI)

    Zhang, Yun

    2008-12-18T23:59:59.000Z

    The purpose of this research was to expand the chemiluminescence microscopy applications in live bacterial/mammalian cell imaging and to improve the detection sensitivity for ATP leaking or release events. We first demonstrated that chemiluminescence (CL) imaging can be used to interrogate single bacterial cells. While using a luminometer allows detecting ATP from cell lysate extracted from at least 10 bacterial cells, all previous cell CL detection never reached this sensitivity of single bacteria level. We approached this goal with a different strategy from before: instead of breaking bacterial cell membrane and trying to capture the transiently diluted ATP with the firefly luciferase CL assay, we introduced the firefly luciferase enzyme into bacteria using the modern genetic techniques and placed the CL reaction substrate D-luciferin outside the cells. By damaging the cell membrane with various antibacterial drugs including antibiotics such as Penicillins and bacteriophages, the D-luciferin molecules diffused inside the cell and initiated the reaction that produces CL light. As firefly luciferases are large protein molecules which are retained within the cells before the total rupture and intracellular ATP concentration is high at the millmolar level, the CL reaction of firefly luciferase, ATP and D-luciferin can be kept for a relatively long time within the cells acting as a reaction container to generate enough photons for detection by the extremely sensitive intensified charge coupled device (ICCD) camera. The result was inspiring as various single bacterium lysis and leakage events were monitored with 10-s temporal resolution movies. We also found a new way of enhancing diffusion D-luciferin into cells by dehydrating the bacteria. Then we started with this novel single bacterial CL imaging technique, and applied it for quantifying gene expression levels from individual bacterial cells. Previous published result in single cell gene expression quantification mainly used a fluorescence method; CL detection is limited because of the difficulty to introduce enough D-luciferin molecules. Since dehydration could easily cause proper size holes in bacterial cell membranes and facilitate D-luciferin diffusion, we used this method and recorded CL from individual cells each hour after induction. The CL light intensity from each individual cell was integrated and gene expression levels of two strain types were compared. Based on our calculation, the overall sensitivity of our system is already approaching the single enzyme level. The median enzyme number inside a single bacterium from the higher expression strain after 2 hours induction was quantified to be about 550 molecules. Finally we imaged ATP release from astrocyte cells. Upon mechanical stimulation, astrocyte cells respond by increasing intracellular Ca{sup 2+} level and releasing ATP to extracellular spaces as signaling molecules. The ATP release imaged by direct CL imaging using free firefly luciferase and D-luciferin outside cells reflects the transient release as well as rapid ATP diffusion. Therefore ATP release detection at the cell surface is critical to study the ATP release mechanism and signaling propagation pathway. We realized this cell surface localized ATP release imaging detection by immobilizing firefly luciferase to streptavidin beads that attached to the cell surface via streptavidin-biotin interactions. Both intracellular Ca{sup 2+} propagation wave and extracellular ATP propagation wave at the cell surface were recorded with fluorescence and CL respectively. The results imply that at close distances from the stimulation center (<120 {micro}m) extracellular ATP pathway is faster, while at long distances (>120 {micro}m) intracellular Ca{sup 2+} signaling through gap junctions seems more effective.

  20. Effects of oil spill dispersants and drilling fluids on substrate specificity of marine bacteria

    SciTech Connect (OSTI)

    Okpokwasili, G.C.; Nnubia, C. [Univ. of Port Harcourt (Nigeria). Dept. of Microbiology] [Univ. of Port Harcourt (Nigeria). Dept. of Microbiology

    1995-12-31T23:59:59.000Z

    The effects of oil spill dispersants and drilling fluids on the sizes of populations of specific heterotroph subgroups of marine bacteria were monitored in this study. The bacteria were isolated from drill cuttings recovered from Agbara--an offshore oilfield located some 100 nautical miles off the Atlantic coast of Nigeria. Numbers of cellulolytic, proteolytic, starch-hydrolyzing and lipolytic bacteria in the drill cuttings were monitored for 28 days in the presence of oil spill dispersants and drilling fluids. The percentages of these bacterial subgroups within the total heterotrophic population enumerated on tryptic soy agar (10% with 3% NaCl) fluctuated between 3.0 and 17.0%, 0.0 and 27.0%, 4.0 and 25.0% and 3.0 and 18.0% for cellulolytic, proteolytic, starch-hydrolyzing and lipolytic bacteria respectively. These results indicate that oil spill dispersants and drilling fluids affect the ability of marine bacteria to metabolize these substrates in the environment.

  1. Direct immunofluorescence and enzyme-linked immunosorbent assays for evaluating chlorinated hydrocarbon degrading bacteria

    SciTech Connect (OSTI)

    Brigmon, R.L.; Franck, M.M.; Brey, J.; Fliermans, C.B. [Westinghouse Savannah River, Aiken, SC (United States). Environmental Biotechnology Section; Scott, D.; Lanclos, K. [Medical Coll. of Georgia, Augusta, GA (United States)

    1997-06-01T23:59:59.000Z

    Immunological procedures were developed to enumerate chlorinated hydrocarbon degrading bacteria. Polyclonal antibodies (Pabs) were produced by immunizing New Zealand white rabbits against 18 contaminant-degrading bacteria. These included methanotrophic and chlorobenzene (CB) degrading species. An enzyme-linked immunosorbent assay (ELISA) was used to test for specificity and sensitivity of the Pabs. Direct fluorescent antibodies (DFAs) were developed with these Pabs against select methanotrophic bacteria isolated from a trichloroethylene (TCE) contaminated landfill at the Savannah River Site (SRS) and cultures from the American Type Culture Collection (ATCC). Analysis of cross reactivity testing data showed some of the Pabs to be group specific while others were species specific. The threshold of sensitivity for the ELISA is 105 bacteria cells/ml. The DFA can detect as few as one bacterium per ml after concentration. Results from the DFA and ELISA techniques for enumeration of methanotrophic bacteria in groundwater were higher but not significantly different (P < 0.05) compared to indirect microbiological techniques such as MPN. These methods provide useful information on in situ community structure and function for bioremediation applications within 1--4 hours of sampling.

  2. Genome sequence of the Fleming strain of Micrococcus luteus, a simple free- living actinobacterium

    SciTech Connect (OSTI)

    Young, Michael; Artsatbanov, Vladislav; Beller, Harry R.; Chandra, Govind; Chater, Keith F.; Dover, Lynn G.; Goh, Ee-Been; Kahan, Tamar; Kaprelyants, Arseny S.; Kyrpides, Nikos; Lapidus, Alla; Lowry, Stephen R.; Lykidis, Athanasios; Mahillon, Jacques; Markowitz, Viktor; Mavrommatis, Konstantinos; Mukamolova, Galina V.; Oren, Aharon; Rokem, J. Stefan; Smith, Margaret C. M.; Young, Danielle I.; Greenblatt, Charles L.

    2009-11-01T23:59:59.000Z

    Micrococcus luteus (NCTC2665, Fleming strain) has one of the smallest genomes of free living actinobacteria sequenced to date, comprising a single circular chromosome of 2,501,097 bp (G+C content 73%) predicted to encode 2403 proteins. The genome shows extensive synteny with that of the closely related organism, Kocuria rhizophila, from which it was taxonomically separated relatively recently. Despite its small size, the genome harbors 73 IS elements, almost all of which are closely related to elements found in other actinobacteria. An IS element is inserted into the rrs gene of one of only two rrn operons found in M. luteus. The genome encodes only four sigma factors and fourteen response regulators, indicative of adaptation to a rather strict ecological niche (mammalian skin). The high sensitivity of M. luteus to {Beta}-lactam antibiotics may result from the presence of a reduced set of penicillin binding proteins and the absence of a wblC gene, which plays an important role in antibiotic resistance in other actinobacteria. Consistent with the restricted range of compounds it can use as a sole source of carbon for energy and growth, M. luteus has a minimal complement of genes concerned with carbohydrate transport and metabolism and its inability to utilize glucose as a sole carbon source may be due to the apparent absence of a gene encoding glucokinase. Uniquely among characterized bacteria, M. luteus appears to be able to metabolize glycogen only via trehalose, and to make trehalose only via glycogen. It has very few genes associated with secondary metabolism. In contrast to other actinobacteria, M. luteus encodes only one resuscitation-promoting factor (Rpf) required for emergence from dormancy and its complement of other dormancy-related proteins is also much reduced. M. luteus is capable of long-chain alkene biosynthesis, which is of interest for advanced biofuel production; a three gene cluster essential for this metabolism has been identified in the genome.

  3. The Sustainability FYE Cluster The Sustainability FYE Cluster will make sustainable urban living a core experience for First

    E-Print Network [OSTI]

    The Sustainability FYE Cluster The Sustainability FYE Cluster will make sustainable urban living University. The Sustainability community infuses the learning-living community with practical and theoretical approaches to sustainable living, merging students' living community with unique academic and field

  4. Liquid Fuel From Renewable Electricity and Bacteria: Electro-Autotrophic Synthesis of Higher Alcohols

    SciTech Connect (OSTI)

    None

    2010-07-01T23:59:59.000Z

    Electrofuels Project: UCLA is utilizing renewable electricity to power direct liquid fuel production in genetically engineered Ralstonia eutropha bacteria. UCLA is using renewable electricity to convert carbon dioxide into formic acid, a liquid soluble compound that delivers both carbon and energy to the bacteria. The bacteria are genetically engineered to convert the formic acid into liquid fuel—in this case alcohols such as butanol. The electricity required for the process can be generated from sunlight, wind, or other renewable energy sources. In fact, UCLA’s electricity-to-fuel system could be a more efficient way to utilize these renewable energy sources considering the energy density of liquid fuel is much higher than the energy density of other renewable energy storage options, such as batteries.

  5. Genetically Modified Bacteria for Fuel Production: Development of Rhodobacteria as a Versatile Platform for Fuels Production

    SciTech Connect (OSTI)

    None

    2010-07-01T23:59:59.000Z

    Electrofuels Project: Penn State is genetically engineering bacteria called Rhodobacter to use electricity or electrically generated hydrogen to convert carbon dioxide into liquid fuels. Penn State is taking genes from oil-producing algae called Botryococcus braunii and putting them into Rhodobacter to produce hydrocarbon molecules, which closely resemble gasoline. Penn State is developing engineered tanks to support microbial fuel production and determining the most economical way to feed the electricity or hydrogen to the bacteria, including using renewable sources of power like solar energy.

  6. The battle of bacteria: Agencies, stakeholders focusing on restoring water quality

    E-Print Network [OSTI]

    Foust, Margaret

    2011-01-01T23:59:59.000Z

    txH2O | pg. 20 Story by Margaret Foust Bacteria is the No. 1 pollutant of water in Texas, causing many of the state?s water bodies to be placed on the Texas Water Quality Inventory and 303(d) List for failing to meet contact recreation use... standards. Across the state, agencies and local stakeholders are identifying the sources of pollution in bacteria-impaired water bodies and are developing management strategies to restore water quality and remove these water bodies from the impaired...

  7. The battle of bacteria: Agencies, stakeholders focusing on restoring water quality 

    E-Print Network [OSTI]

    Foust, Margaret

    2010-01-01T23:59:59.000Z

    txH2O | pg. 20 Story by Margaret Foust Bacteria is the No. 1 pollutant of water in Texas, causing many of the state?s water bodies to be placed on the Texas Water Quality Inventory and 303(d) List for failing to meet contact recreation use... standards. Across the state, agencies and local stakeholders are identifying the sources of pollution in bacteria-impaired water bodies and are developing management strategies to restore water quality and remove these water bodies from the impaired...

  8. The possible symbiotic role of proteolytic and cellulolytic bacteria found in the gut of Limnoria 

    E-Print Network [OSTI]

    Traganza, Eugene Dewees

    1959-01-01T23:59:59.000Z

    THE POSSIBLE SYMBIOTIC ROLE OF PROTEOLYTIC AND CELLVLOLYTIC BACTERIA FOUND IN TBE GV1' OF LIINORIA A Thesis By Eugene Dewees Traganza ~ ~ I Submitted to the Graduate School of the Agricultural and Mechanical College of Texas in partial... fulfillment of the requirements for the degree of MASTER OF SCIENCE January 1959 Ma)or Sub5ect: Biological Oceanography THE POSSIBLE SYMBIO1'IC ROLE OF PROTEOLYTIC AND CELLULOLYTIC BACTERIA FOUND IN THE GU1' OF Like(RIA A Thesis By Eugene Dewees...

  9. Investigation of anaerobic bacteria associated with mortality in grey mullet (Mugil cephalus) and redfish (Sciaenops ocellatus) 

    E-Print Network [OSTI]

    Henley, Michael Waite

    1976-01-01T23:59:59.000Z

    INVEST1GATION OF ANAEROBIC BACTERIA ASSOCIATED WITH NDRTALITY IN GREY MULLET t~N' I ~*h I ) AND REDYIRH (~H' o ll t A Thesis MICHAEL WAITE HENLEY f Submitted to the Graduate College of Texas ARM University in partial fulfillment... of the requirement for the degree of MASTER OF SCIENCE August 1976 Major Subject: Veterinary Microbiology INVESTIGATION OF ANAEROBIC BACTERIA ASSOCIATED NITH liIIH NDR IlDIIY IN GREY NUDE EI 1M~I ~ll END REDPI H l~R' ll t 1 A Thesis !G!ICHAEL WAITE HENLEY...

  10. Factors affecting the recovery of bacteria in freeze-dried model systems 

    E-Print Network [OSTI]

    Custer, Carl Steven

    1970-01-01T23:59:59.000Z

    FACTORS AFFECTING THE RECOVERY OF BACTERIA IN FREEZE-DRIED NODEL SYSTENS A Thesis by CARL STEVEN CUSTER Submitted to the Graduate College of Texas A&N University in partial fulfillment of the requirement for tbe degree of MASTER OF SCIENCE... December 1970' Najor Subject: Food Technology FACTORS AFFECTING THE RECOVERY OF BACTERIA IN FREEZE-DRIED MODEL SYSTEMS A Thesis CARL STEVEN CUSTER Approved as to sty1e and content by: Chairman of Com tee) Head of Departme (Member) (Member) (Member...

  11. The battle of bacteria: Agencies, stakeholders focusing on restoring water quality 

    E-Print Network [OSTI]

    Foust, Margaret

    2011-01-01T23:59:59.000Z

    txH2O | pg. 20 Story by Margaret Foust Bacteria is the No. 1 pollutant of water in Texas, causing many of the state?s water bodies to be placed on the Texas Water Quality Inventory and 303(d) List for failing to meet contact recreation use... standards. Across the state, agencies and local stakeholders are identifying the sources of pollution in bacteria-impaired water bodies and are developing management strategies to restore water quality and remove these water bodies from the impaired...

  12. Genomics Encyclopedia of Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB): a resource for microsymbiont genomes (2013 DOE JGI Genomics of Energy and Environment 8th Annual User Meeting)

    SciTech Connect (OSTI)

    Reeve, Wayne [Murdoch University

    2013-03-01T23:59:59.000Z

    Wayne Reeve of Murdoch University on "Genomics Encyclopedia of Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB): a resource for microsymbiont genomes" at the 8th Annual Genomics of Energy & Environment Meeting on March 27, 2013 in Walnut Creek, Calif.

  13. Women in management: gender, age and working lives 

    E-Print Network [OSTI]

    Jyrkinen, Marjut; McKie, Linda

    The project reported in this briefing paper explored how age and gender inter-weave and impact on the working and home lives of women managers working in two EU member states, namely Finland and Scotland. The proportion of women in middle...

  14. Safety and Liveness in Intelligent Intersections Hemant Kowshik1

    E-Print Network [OSTI]

    of designing intelligent intersections where traffic lights and stop signs are removed, and cars negotiate intersections are representative of complex distributed hybrid systems which need architectures and algorithms with provable safety and liveness. We propose a hybrid architecture which involves an appropriate inter- play

  15. Distribution of ranks of ?-decay half-lives

    E-Print Network [OSTI]

    Juan Miguel Campanario

    2010-11-21T23:59:59.000Z

    I studied the distribution of ranks of values of 2949 {\\beta}-decay half-lives according to an empirical beta law with two exponents. {\\beta}-decay half-life ranks showed good fit to a beta function with two exponents.

  16. Adaptive Traffic-Based Techniques For Live Multimedia Streaming

    E-Print Network [OSTI]

    Adaptive Traffic-Based Techniques For Live Multimedia Streaming Gabriel-Miro Muntean and LiamMiro.Muntean@ucd.ie, Liam.Murphy@ucd.ie Abstract The growing number of multimedia stream transmissions done through the time-sensitive applications and especially the continuity of multimedia streaming. The paper presents

  17. Live in Spain while earning academic credit Spring 2013

    E-Print Network [OSTI]

    + + + Live in Spain while earning academic credit Spring 2013 · Study at one of Spain's oldest Spain with other CSU students · Take advantage of the opportunities you will have to travel throughout Spain & greater Europe Centrally located 20 miles from the vibrant city of Madrid, Alcalá de Henares

  18. LIVE. WORK. CELEBRATE. Email order to: Beverlyhills@cort.com

    E-Print Network [OSTI]

    Williams, Gary A.

    and Lamp LIVING ROOM: Sofa, Chair, Cocktail Table, End Table, Lamp, plus 32" LCD TV with Stand STUDY: Chair TV with Stand, Accent Pillows and 3 Pieces of Artwork STUDY: Chair and Lamp DINING ROOM: Dining Table drop it off, set it up and pick it up n No heavy lifting or assembly required n Fit a student

  19. ith America focused on energy alternatives and green living,

    E-Print Network [OSTI]

    Veiga, Pedro Manuel Barbosa

    W ith America focused on energy alternatives and green living, Oklahoma State University- Tulsa. His process to produce a clean, alternative energy source could significantly reduce the use of fossil it a very attractive alternative to solar energy technology. "Thermoelectric materials are so much less

  20. http://tti.tamu.edu Saving Lives, Time and Resources

    E-Print Network [OSTI]

    and organization > Project management > Education and training > Costs > Capital investments > Road pricinghttp://tti.tamu.edu Saving Lives, Time and Resources A Member of The Texas A&M University System estimation > Traffic forecasting > Commuting > Carpools > Highway operations > Highway capacity > Freeway

  1. Green Living At Dartmouth College BUILDINGS, COMMUNITY AND THE

    E-Print Network [OSTI]

    Green Living At Dartmouth College BUILDINGS, COMMUNITY AND THE ENVIRONMENT Environmental Studies 50 steps necessary for this goal. We begin by defining "green building" and explaining its relevance to the College's plans for new residences. Green building incorporates sensitivity towards the environment

  2. Green Living At Dartmouth College BUILDINGS, COMMUNITY AND THE

    E-Print Network [OSTI]

    Green Living At Dartmouth College BUILDINGS, COMMUNITY AND THE ENVIRONMENT Environmental Studies 50 Recommendations 3 a. Chapters I and II: Green Building and New Dorms 3 b. Chapter III: Existing Dorms 3 c. Chapter Institutions 5 CHAPTER 2: GREEN BUILDING 6 CHAPTER 3: NEW DORMS 9 I. The Need for New Housing 10 II. Overview

  3. Performance and Energy Modeling for Live Migration of Virtual Machines

    E-Print Network [OSTI]

    Xu, Cheng-Zhong

    Performance and Energy Modeling for Live Migration of Virtual Machines Haikun Liu , Cheng-Zhong Xu , Hai Jin , Jiayu Gong , Xiaofei Liao School of Computer Science and Technology Huazhong University of Science and Technology Wuhan, 430074, China {hjin, xfliao}@hust.edu.cn Department of Electrical

  4. Residential Services Area Missing Students living in University Managed Accommodation

    E-Print Network [OSTI]

    Sussex, University of

    Residential Services Area Missing Students living in University Managed Accommodation 1.0 Where then report to the Building Manager or to the Residential Student Support Team or the Residential Services issues Residential Student Support Manager or the Residential Services Manager should be contacted

  5. Vertebrate heart development: Lessons learnt from live imaging

    E-Print Network [OSTI]

    Shyamasundar, R.K.

    Vertebrate heart development: Lessons learnt from live imaging California Institute of Technology employing different imaging techniques. Sub resolution imaging of beating zebrafish heart has however remained a challenge owing Embryonic heart is a 100 moving quasi-periodically at few Hertz frequency, over

  6. The importance of live biofilms in corrosion protection

    E-Print Network [OSTI]

    Wood, Thomas K.

    Letter The importance of live biofilms in corrosion protection Rongjun Zuo a , Esra Kus b , Florian, University of Connecticut, 191 Auditorium Road, U-3222, Storrs, CT 06269-3222, USA b Corrosion occurred within a few hours as indicated by characteristic changes in the impedance spectra. The corrosion

  7. Healthy Foods, Healthy Lives: Cooking on a Student's Budget

    E-Print Network [OSTI]

    Amin, S. Massoud

    Healthy Foods, Healthy Lives: Cooking on a Student's Budget FScN 2002 ­ Fall & Spring Semesters/monthly menus within a realistic food budget and a modest supply list Gain valuable information and connect to a variety of resources on nutrition, food safety, budgeting and meal planning Develop a social network

  8. PUBLISHED ONLINE: 7 JULY 2013 | DOI: 10.1038/NPHYS2676 Bacteria can exploit a flagellar buckling instability

    E-Print Network [OSTI]

    Loss, Daniel

    in the locomotion of bacteria with multiple flagella (peritrichous), such as Escherichia coli, which bundles its a single flagellum (monotrichous), including 90% of motile marine bacteria3 , and how they reorient has long remained unclear. Only recently has the monotrichous marine bacterium Vibrio alginolyticus been

  9. Digestion of milk protein and methanol-grown bacteria protein in the preruminant calf. II. Amino acid composition of

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Digestion of milk protein and methanol-grown bacteria protein in the preruminant calf. II. Amino of milk and of methanol-grown bacteria in the terminal small intestine and the hindgut of the preruminant exclusively by skim-milk powder ; 50.5 p. 100 of the protein of the bacte- ria diet was supplied by methanol

  10. ADENOSINE TRIPHOSPHATE (ATP) AND DEOXYRIBONUCLEIC ACID (DNA) CONTENT OF MARINE MICROALGAE AND BACTERIA WITH

    E-Print Network [OSTI]

    Luther, Douglas S.

    ADENOSINE TRIPHOSPHATE (ATP) AND DEOXYRIBONUCLEIC ACID (DNA) CONTENT OF MARINE MICROALGAE the relationship between DNA and ATP content of marine bacteria and microalgae. This relationship was used. Laboratory-derived DNA:ATP ratios ranged from 8.5 to 33 (wt:wt) for cultures of marine microalgae, and from

  11. Microfluidic capture and release of bacteria in a conical nanopore array Peng Guo,ab

    E-Print Network [OSTI]

    Zare, Richard N.

    Microfluidic capture and release of bacteria in a conical nanopore array Peng Guo,ab Eric W. Hall a microfluidic device. As an example, we demonstrate that cyanobacteria can be captured, one bacterium per pore, in a conical nanoporous membrane (CNM) integrated into a microfluidic chip. This study, to our knowledge

  12. A conjecture on the relationship of bacterial shape to motility in rod-shaped bacteria

    E-Print Network [OSTI]

    Denny, Mark

    ; Escherichia coli; Hydrodynamic e¤ciency 1. Introduction After we ¢rst notice that rod-shaped bacteria, for example, why does Escherichia coli have the shape it does? Why isn't it longer or shorter? 1.1. A priori School, Ann Arbor, MI 48109-0620, USA b Department of Biology, Stanford University, Hopkins Marine

  13. Rapid chemotactic response enables marine bacteria to exploit ephemeral microscale nutrient patches

    E-Print Network [OSTI]

    Entekhabi, Dara

    chemotaxis model Escherichia coli, leading to twice the nutrient exposure. We demonstrate that such rapidRapid chemotactic response enables marine bacteria to exploit ephemeral microscale nutrient patches mechanisms dissipate them. Here we show that the rapid chemo- tactic response of the marine bacterium

  14. Transfer of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota

    E-Print Network [OSTI]

    Gleeson, Joseph G.

    in Escherichia coli. However, only Zg1017 and the catalytic module of Zg2600 were expressed as soluble proteinsLETTERS Transfer of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota Jan of the marine Bacteroidetes, Zobellia galactanivorans, active on the sulphated polysaccharide porphyran from

  15. Roles of Naturally Occurring Bacteria in Controlling Iodine-129 Mobility in Subsurface Soils

    E-Print Network [OSTI]

    Li, Hsiu-Ping

    2012-10-19T23:59:59.000Z

    - to reactive intermediates. The aim of this study was to determine the influence of naturally occurring aerobic bacteria isolated from an 129I contaminated aquifer (F-area of the Savannah River Site, SC) on I- oxidation and OI formation. It was demonstrated...

  16. Structure-based Inhibitor Discovery against Adenylyl Cyclase Toxins from Pathogenic Bacteria That Cause Anthrax and

    E-Print Network [OSTI]

    Mrksich, Milan

    That Cause Anthrax and Whooping Cough* Received for publication, February 4, 2003, and in revised form, March bacteria that cause anthrax and whooping cough, respectively. Using the structure of the catalytic site pathogenesis and to fight against anthrax and whooping cough. The 2001 anthrax attacks in the United States

  17. Winery waste makes fuel Electricity, bacteria break organics in wastewater into hydrogen gas

    E-Print Network [OSTI]

    from agricultural wastes." Napa Wine Company's wastewater comes from grape disposal, wine makingMSNBC.com Winery waste makes fuel Electricity, bacteria break organics in wastewater into hydrogen method for generating hydrogen fuel from wastewater is now operating at a California winery

  18. ORNL researchers are studying how bacteria found in Yellowstone hot springs

    E-Print Network [OSTI]

    Pennycook, Steve

    deconstruction--a major roadblock to the production of advanced biofuels," Giannone said. --Morgan Mc in the development of a cheaper biofuel production process. (Image: Jennifer Morrell-Falvey/ORNL) SCIENCE ORNL explores proteins in Yellowstone bacteria for biofuel inspiration substrates such as pure cellulose

  19. A phylogenetic mixture model for gene family loss in parasitic bacteria

    E-Print Network [OSTI]

    A phylogenetic mixture model for gene family loss in parasitic bacteria Submitted as a research words: gene families, mixture model, parasites, maximum likelihood,phylogenetics Running head: gene family gain and loss 1 #12;Abstract Gene families are frequently gained and lost from prokaryotic genomes

  20. Additive and Competitive Effects of Bacteria and Mn Oxides on Arsenite Oxidation Kinetics

    E-Print Network [OSTI]

    Sparks, Donald L.

    Additive and Competitive Effects of Bacteria and Mn Oxides on Arsenite Oxidation Kinetics L oxidizing As(III) at the same time than for either component alone. The additive effect of the mixed cell even though As(III) was oxidized fastest in a mixed cell--MnO2 system. The additive effect of biotic

  1. in-house windrow composting Many of the water bodies that are impaired by bacteria

    E-Print Network [OSTI]

    in-house windrow composting Many of the water bodies that are impaired by bacteria throughout from new and existing facilities. One such BMP is in-house windrow composting (IWC) of poultry litter. The Environmental Effects of In-House Windrow Composting of Poultry Litter project is demonstrating

  2. Bacteria can sprout webs of electrical wiring that transform the soil into a geological

    E-Print Network [OSTI]

    Lovley, Derek

    cells into a web-like electrical circuit, they report (D. Ntarlagiannis et al. Geophys. Res. Lett. 34, L in La Jolla, California. As the bacteria consume nutrients for energy, they spit out unwanted electrons sand infiltrated with a nutrient compound (lactate), and allowed S. oneidensis to grow in this `fake

  3. aerobic hydrogen-consuming bacteria: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    aerobic hydrogen-consuming bacteria First Page Previous Page 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 Diversity of...

  4. Growth Temperature and Genome Size in Bacteria Are Negatively Correlated, Suggesting Genomic Streamlining

    E-Print Network [OSTI]

    Wagner, Andreas

    Growth Temperature and Genome Size in Bacteria Are Negatively Correlated, Suggesting Genomic.wagner@ieu.uzh.ch; nsabath@gmail.com. Accepted: March 25, 2013 Abstract Prokaryotic genomes are small and compact. Either this feature is caused by neutral evolution or by natural selection favoring small genomes--genome streamlining

  5. How do microbial fuel cells (MFCs) work? Bacteria need energy to survive, in the same way that humans need food to

    E-Print Network [OSTI]

    Lee, Dongwon

    How do microbial fuel cells (MFCs) work? Bacteria need energy to survive, in the same way" in photobiological fuel cell systems that utilize photosynthetic bacteria, amino acids, and proteins. What

  6. ORGANIC GEOCHEMICAL STUDIES. II. THE DISTRIBUTION OF ALIPHATIC HYDROCARBONS IN ALGAE, BACTERIA, AND IN A RECENT LAKE SEDIMENT: A PRELIMINARY REPORT

    E-Print Network [OSTI]

    Han, Jerry; McCarthy, E.D.; Van Hoeven Jr., William; Calvin, Melvin; Bradley, W. H.

    2008-01-01T23:59:59.000Z

    ALIPHATIC HYDROCARBONS IN ALGAE, BACTERIA, AND IN A RECENTH F A PRELIMINARY REPORT IN ALGAE, BACTERIA, AKD IN A RECENTrests on the finding that algae have less cellulose and a

  7. From Nanowires to Biofilms: An Exploration of Novel Mechanisms of Uranium Transformation Mediated by Geobacter Bacteria

    SciTech Connect (OSTI)

    REGUERA, GEMMA [Michigan State University

    2014-01-16T23:59:59.000Z

    One promising strategy for the in situ bioremediation of radioactive groundwater contaminants that has been identified by the SBR Program is to stimulate the activity of dissimilatory metal-reducing microorganisms to reductively precipitate uranium and other soluble toxic metals. The reduction of U(VI) and other soluble contaminants by Geobacteraceae is directly dependent on the reduction of Fe(III) oxides, their natural electron acceptor, a process that requires the expression of Geobacter’s conductive pili (pilus nanowires). Expression of conductive pili by Geobacter cells leads to biofilm development on surfaces and to the formation of suspended biogranules, which may be physiological closer to biofilms than to planktonic cells. Biofilm development is often assumed in the subsurface, particularly at the matrix-well screen interface, but evidence of biofilms in the bulk aquifer matrix is scarce. Our preliminary results suggest, however, that biofilms develop in the subsurface and contribute to uranium transformations via sorption and reductive mechanisms. In this project we elucidated the mechanism(s) for uranium immobilization mediated by Geobacter biofilms and identified molecular markers to investigate if biofilm development is happening in the contaminated subsurface. The results provided novel insights needed in order to understand the metabolic potential and physiology of microorganisms with a known role in contaminant transformation in situ, thus having a significant positive impact in the SBR Program and providing novel concept to monitor, model, and predict biological behavior during in situ treatments.

  8. Lévy Fluctuations and Tracer Diffusion in Dilute Suspensions of Algae and Bacteria

    E-Print Network [OSTI]

    Irwin M. Zaid; Jörn Dunkel; Julia M. Yeomans

    2010-09-20T23:59:59.000Z

    Swimming microorganisms rely on effective mixing strategies to achieve efficient nutrient influx. Recent experiments, probing the mixing capability of unicellular biflagellates, revealed that passive tracer particles exhibit anomalous non-Gaussian diffusion when immersed in a dilute suspension of self-motile Chlamydomonas reinhardtii algae. Qualitatively, this observation can be explained by the fact that the algae induce a fluid flow that may occasionally accelerate the colloidal tracers to relatively large velocities. A satisfactory quantitative theory of enhanced mixing in dilute active suspensions, however, is lacking at present. In particular, it is unclear how non-Gaussian signatures in the tracers' position distribution are linked to the self-propulsion mechanism of a microorganism. Here, we develop a systematic theoretical description of anomalous tracer diffusion in active suspensions, based on a simplified tracer-swimmer interaction model that captures the typical distance scaling of a microswimmer's flow field. We show that the experimentally observed non-Gaussian tails are generic and arise due to a combination of truncated L\\'evy statistics for the velocity field and algebraically decaying time correlations in the fluid. Our analytical considerations are illustrated through extensive simulations, implemented on graphics processing units to achieve the large sample sizes required for analyzing the tails of the tracer distributions.

  9. Axion cosmology with long-lived domain walls

    SciTech Connect (OSTI)

    Hiramatsu, Takashi [Yukawa Institute for Theoretical Physics, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502 (Japan); Kawasaki, Masahiro; Saikawa, Ken'ichi [Institute for Cosmic Ray Research, The University of Tokyo, 5-1-5 Kashiwa-no-ha, Kashiwa City, Chiba 277-8582 (Japan); Sekiguchi, Toyokazu, E-mail: hiramatz@yukawa.kyoto-u.ac.jp, E-mail: kawasaki@icrr.u-tokyo.ac.jp, E-mail: saikawa@icrr.u-tokyo.ac.jp, E-mail: sekiguti@a.phys.nagoya-u.ac.jp [Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya City, Aichi 464-8602 (Japan)

    2013-01-01T23:59:59.000Z

    We investigate the cosmological constraints on axion models where the domain wall number is greater than one. In these models, multiple domain walls attached to strings are formed, and they survive for a long time. Their annihilation occurs due to the effects of explicit symmetry breaking term which might be raised by Planck-scale physics. We perform three-dimensional lattice simulations and compute the spectra of axions and gravitational waves produced by long-lived domain walls. Using the numerical results, we estimated relic density of axions and gravitational waves. We find that the existence of long-lived domain walls leads to the overproduction of cold dark matter axions, while the density of gravitational waves is too small to observe at the present time. Combining the results with other observational constraints, we find that the whole parameter region of models are excluded unless an unacceptable fine-tuning exists.

  10. NUCLEAR HALF-LIVES FOR ALPHA RADIOACTIVITY OF ELEMENTS WITH

    E-Print Network [OSTI]

    P. Roy Chowdhury A; C. Samanta A

    Theoretical estimates for the half lives of about 1700 isotopes of heavy elements with 100 ? Z ? 130 are tabulated using theoretical Q-values. The quantum mechanical tunneling probabilities are calculated within a WKB framework using microscopic nuclear potentials. The microscopic nucleus- nucleus potentials are obtained by folding the densities of interacting nuclei with a density dependent M3Y (DDM3Y) effective nucleon- nucleon interaction. The ?-decay half lives calculated in this formalism using the experimental Q-values were found to be in good agreement over a wide range of experimental data spanning about twenty orders of magnitude. The theoretical Q-values used for the present calculations are extracted from three different mass estimates viz. Myers-Swiatecki [MS], Muntian-Hofmann-Patyk-

  11. The problem of living in a world contaminated with chemicals

    SciTech Connect (OSTI)

    Metcalf, R.L. [Univ. of Illinois, Urbana (United States)

    1990-12-31T23:59:59.000Z

    The proliferation of xenobiotic chemicals in the global environment poses living problems for each of us aboard {open_quotes}spaceship earth.{close_quotes} Seven case studies are presented that illustrate the magnitude of the problem that can result from waiting to identify toxic hazards until there have been decades of {open_quotes}human guinea pig{close_quotes} exposure. 25 refs., 5 tabs.

  12. Single-photon ultrashort-lived radionuclides: symposium proceedings

    SciTech Connect (OSTI)

    Paras, P.; Thiessen, J.W. (eds.)

    1985-01-01T23:59:59.000Z

    The purpose was to define the current role and state-of-the-art regarding the development, clinical applications, and usefulness of generator-produced single-photon ultrashort-lived radionuclides (SPUSLR's) and to predict their future impact on medicine. Special emphasis was placed on the generator production of iridium-191, gold-195, and krypton-81. This report contains expanded summaries of the included papers. (ACR)

  13. Dynamic self-assembly in living systems as computation.

    SciTech Connect (OSTI)

    Bouchard, Ann Marie; Osbourn, Gordon Cecil

    2004-06-01T23:59:59.000Z

    Biochemical reactions taking place in living systems that map different inputs to specific outputs are intuitively recognized as performing information processing. Conventional wisdom distinguishes such proteins, whose primary function is to transfer and process information, from proteins that perform the vast majority of the construction, maintenance, and actuation tasks of the cell (assembling and disassembling macromolecular structures, producing movement, and synthesizing and degrading molecules). In this paper, we examine the computing capabilities of biological processes in the context of the formal model of computing known as the random access machine (RAM) [Dewdney AK (1993) The New Turing Omnibus. Computer Science Press, New York], which is equivalent to a Turing machine [Minsky ML (1967) Computation: Finite and Infinite Machines. Prentice-Hall, Englewood Cliffs, NJ]. When viewed from the RAM perspective, we observe that many of these dynamic self-assembly processes - synthesis, degradation, assembly, movement - do carry out computational operations. We also show that the same computing model is applicable at other hierarchical levels of biological systems (e.g., cellular or organism networks as well as molecular networks). We present stochastic simulations of idealized protein networks designed explicitly to carry out a numeric calculation. We explore the reliability of such computations and discuss error-correction strategies (algorithms) employed by living systems. Finally, we discuss some real examples of dynamic self-assembly processes that occur in living systems, and describe the RAM computer programs they implement. Thus, by viewing the processes of living systems from the RAM perspective, a far greater fraction of these processes can be understood as computing than has been previously recognized.

  14. ClimateChangeLIVE Webcast: Join the Climate Conversation

    Broader source: Energy.gov [DOE]

    Join ClimateChangeLIVE's webcast, bringing together students and climate experts for a discussion about climate change and what students and classes around the country are doing to be part of the climate solution. Students will be able to interact with climate scientists and experts online through Facebook and Twitter. A GreenWorks! grant will be offered to help schools with climate action projects.

  15. Half-lives of Double $?^+$-decay with Two Neutrinos

    E-Print Network [OSTI]

    Yuejiao Ren; Zhongzhou Ren

    2015-01-07T23:59:59.000Z

    Nuclear double $\\beta ^-$-decays with two neutrinos were observed for many years and a systematic law describing the relation between their half-lives and decay energies was also proposed recently [Phys. Rev. C89, 064603 (2014)]. However, double $\\beta ^+$-decay ($\\beta ^+\\beta^+)$ with emission of both two positrons and two neutrinos has not been observed up to date. In this article, we perform a systematic analysis on the candidates of double $\\beta ^+$-decay, based on the 2012 nuclear mass table. Eight nuclei are found to be the good candidates for double $\\beta ^+$-decay and their half-lives are predicted according to the generalization of the systematic law to double $\\beta ^+$-decay. As far as we know, there is no theoretical result on double $\\beta ^+$-decay of nucleus $^{154}Dy$ and our result is the first prediction on this nucleus. This is also the first complete research on eight double $\\beta ^+$-decay candidates based on the available data of nuclear masses. It is expected that the calculated half-lives of double $\\beta ^+$-decay in this article will be useful for future experimental search of double $\\beta ^+$-decay.

  16. Mass Spectral Molecular Networking of Living Microbial Colonies

    SciTech Connect (OSTI)

    Watrous, Jeramie D.; Roach, Patrick J.; Alexandrov, Theodore; Heath, Brandi S.; Yang, Jane Y.; Kersten, Roland; vander Voort, Menno; Pogliano, Kit; Gross, Harald; Raaijmakers, Jos M.; Moore, Bradley S.; Laskin, Julia; Bandeira, Nuno; Dorrestein, Pieter C.

    2012-06-26T23:59:59.000Z

    Integrating the governing chemistry with the genomics and phenotypes of microbial colonies has been a "holy grail" in microbiology. This work describes a highly sensitive, broadly applicable, and costeffective approach that allows metabolic profiling of live microbial colonies directly from a Petri dish without any sample preparation. Nanospray desorption electrospray ionization mass spectrometry (MS), combined with alignment of MS data and molecular networking, enabled monitoring of metabolite production from live microbial colonies from diverse bacterial genera, including Bacillus subtilis, Streptomyces coelicolor, Mycobacterium smegmatis, and Pseudomonas aeruginosa. This work demonstrates that, by using these tools to visualize small molecular changes within bacterial interactions, insights can be gained into bacterial developmental processes as a result of the improved organization of MS/MS data. To validate this experimental platform, metabolic profiling was performed on Pseudomonas sp. SH-C52, which protects sugar beet plants from infections by specific soil-borne fungi [R. Mendes et al. (2011) Science 332:1097–1100]. The antifungal effect of strain SHC52 was attributed to thanamycin, a predicted lipopeptide encoded by a nonribosomal peptide synthetase gene cluster. Our technology, in combination with our recently developed peptidogenomics strategy, enabled the detection and partial characterization of thanamycin and showed that it is amonochlorinated lipopeptide that belongs to the syringomycin family of antifungal agents. In conclusion, the platform presented here provides a significant advancement in our ability to understand the spatiotemporal dynamics of metabolite production in live microbial colonies and communities.

  17. Arsenic exposure in children living near a former copper smelter

    SciTech Connect (OSTI)

    Binder, S.; Forney, D.; Kaye, W.; Paschal, D.

    1987-07-01T23:59:59.000Z

    About 10,000 people live in communities surrounding the former copper smelter at Anaconda, Montana. Most of these people live in the town of Anaconda, which is generally upwind of the smelter. The smelter ceased operations in 1980, after almost a century of ore processing. Soil and dust on the smelter site and in the vicinity remain contaminated with arsenic, although at this time air and drinking water arsenic levels are not elevated. Results of soil and dust sampling for arsenic in the communities around the smelter are reported. In the town of Anaconda, surface soil arsenic levels from residential sites have averaged around 100 ppm or greater. Young children are generally believed to be the population with the most nonoccupational exposure to soil. Several models of exposure to environmental arsenic in the Anaconda area have predicted that children living in all communities surrounding the smelter would be having significant and measurable exposure to arsenic. Two exposures surveys, conducted while the smelter was operative, demonstrated that excess exposure to arsenic was occurring in young children. Until the present surveys, no exposure data had been collected since the smelter was closed.

  18. Production of Crosslinked, Hollow Nanoparticles by Surface-Initiated Living Free-Radical Polymerization

    E-Print Network [OSTI]

    Harth, Eva M.

    Production of Crosslinked, Hollow Nanoparticles by Surface-Initiated Living Free alkoxyamine initiating groups are attached to the surface silanol groups of silica nanoparticles. This surface Keywords: living radical; nitroxide; nanoparticles; crosslinking; core­shell; poly- mers; nanotechnology

  19. Tribal Summit Live: 9:30-10 AM ET Today and All Day Tomorrow...

    Broader source: Energy.gov (indexed) [DOE]

    Summit Live: 9:30-10 AM ET Today and All Day Tomorrow Tribal Summit Live: 9:30-10 AM ET Today and All Day Tomorrow May 4, 2011 - 8:00am Addthis Ginny Simmons Ginny Simmons Former...

  20. How do Communities Change their Culture Towards more Sustainable Patterns of Living, Working and Learning?

    E-Print Network [OSTI]

    How do Communities Change their Culture Towards more Sustainable Patterns of Living, Working towards more sustainable ways of living; a case study of the Sustainability Office's efforts to change Staff: Heather Scholefield, Strategy Manager, Sustainability Office, Land and Building Services

  1. Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals

    DOE Patents [OSTI]

    Gambhir; Sanjiv (Portola Valley, CA), Pritha; Ray (Mountain View, CA)

    2009-04-28T23:59:59.000Z

    Novel double and triple fusion reporter gene constructs harboring distinct imageable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

  2. Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals

    DOE Patents [OSTI]

    Gambhir, Sanjiv (Portola Valley, CA); Pritha, Ray (Mountain View, CA)

    2011-06-07T23:59:59.000Z

    Novel double and triple fusion reporter gene constructs harboring distinct imagable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

  3. Live Imaging Study on Cytokinin Function and Regulation in Stem-cell Homeostasis

    E-Print Network [OSTI]

    Xie, Mingtang

    2010-01-01T23:59:59.000Z

    on research on SAM Live-image technology was developed toin SAMs, transient perturbations, live image and cell typetreated. (C) The images from the same SAM were used to score

  4. Watch Live: National Science Bowl - Starting At 9:30 AM ET |...

    Energy Savers [EERE]

    Watch Live: National Science Bowl - Starting At 9:30 AM ET Watch Live: National Science Bowl - Starting At 9:30 AM ET May 2, 2011 - 8:55am Addthis Ginny Simmons Ginny Simmons...

  5. X-ray Computed Tomography of the Root System of a Live Potted...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    X-ray Computed Tomography of the Root System of a Live Potted Plant X-ray Computed Tomography of the Root System of a Live Potted Plant Being able to see the root structure of a...

  6. TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing...

    Office of Environmental Management (EM)

    TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's Oil Dependence Through Innovation TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing...

  7. Sustained high levels of neuregulin-1 in the longest-lived rodents; a key determinant of rodent longevity

    E-Print Network [OSTI]

    Huchon, Dorothée

    Sustained high levels of neuregulin-1 in the longest-lived rodents; a key determinant of rodent 31905, Israel Summary Naked mole-rats (Heterocephalus glaber), the longest-lived rodents, live 7% of their lives. Little is known about the mechanisms that allow them to delay the aging process and live so long

  8. Turning Bacteria into Fuel: Cyanobacteria Designed for Solar-Powered Highly Efficient Production of Biofuels

    SciTech Connect (OSTI)

    None

    2010-01-01T23:59:59.000Z

    Broad Funding Opportunity Announcement Project: ASU is engineering a type of photosynthetic bacteria that efficiently produce fatty acids—a fuel precursor for biofuels. This type of bacteria, called Synechocystis, is already good at converting solar energy and carbon dioxide (CO2) into a type of fatty acid called lauric acid. ASU has modified the organism so it continuously converts sunlight and CO2 into fatty acids—overriding its natural tendency to use solar energy solely for cell growth and maximizing the solar-to-fuel conversion process. ASU’s approach is different because most biofuels research focuses on increasing cellular biomass and not on excreting fatty acids. The project has also identified a unique way to convert the harvested lauric acid into a fuel that can be easily blended with existing transportation fuels.

  9. Inoculation of beef with lactic-acid bacteria prior to vacuum packaging

    E-Print Network [OSTI]

    Hall, Lester Cedric

    1978-01-01T23:59:59.000Z

    d of Department ) (Member) August 1978 ABSTRACT Inoculation of Beef with Lactic-Acid Bacteria Prior to Vacuum Packaging. (August 1978) Lester Cedric Hall, B. S. , Texas A&M University Co-Chairmen of Advisory Committee: Dr. G. C. Smith and Dr Z... packaging was rapidly becoming an established business and would revolu- tionize the future of the wholesale beef market. Vacuum packaging is advantageous in that it (a) reduces weight losses from evaporation and trimming, (b) preserves meat color, (c...

  10. Improved oil recovery using bacteria isolated from North Sea petroleum reservoirs

    SciTech Connect (OSTI)

    Davey, R.A.; Lappin-Scott, H. [Univ. of Exeter (United Kingdom)

    1995-12-31T23:59:59.000Z

    During secondary oil recovery, water is injected into the formation to sweep out the residual oil. The injected water, however, follows the path of least resistance through the high-permeability zones, leaving oil in the low-permeability zones. Selective plugging of these their zones would divert the waterflood to the residual oil and thus increase the life of the well. Bacteria have been suggested as an alternative plugging agent to the current method of polymer injection. Starved bacteria can penetrate deeply into rock formations where they attach to the rock surfaces, and given the right nutrients can grow and produce exo-polymer, reducing the permeability of these zones. The application of microbial enhanced oil recovery has only been applied to shallow, cool, onshore fields to date. This study has focused on the ability of bacteria to enhance oil recovery offshore in the North Sea, where the environment can be considered extreme. A screen of produced water from oil reservoirs (and other extreme subterranean environments) was undertaken, and two bacteria were chosen for further work. These two isolates were able to grow and survive in the presence of saline formation waters at a range of temperatures above 50{degrees}C as facultative anaerobes. When a solution of isolates was passed through sandpacks and nutrients were added, significant reductions in permeabilities were achieved. This was confirmed in Clashach sandstone at 255 bar, when a reduction of 88% in permeability was obtained. Both isolates can survive nutrient starvation, which may improve penetration through the reservoir. Thus, the isolates show potential for field trials in the North Sea as plugging agents.

  11. Impact of elevated nitrate on sulfate-reducing bacteria: A comparative study of Desulfovibrio vulgaris

    SciTech Connect (OSTI)

    He, Q.; He, Z.; Joyner, D.C.; Joachimiak, M.; Price, M.N.; Yang, Z.K.; Yen, H.-C. B.; Hemme, C. L.; Chen, W.; Fields, M.; Stahl, D. A.; Keasling, J. D.; Keller, M.; Arkin, A. P.; Hazen, T. C.; Wall, J. D.; Zhou, J.

    2010-07-15T23:59:59.000Z

    Sulfate-reducing bacteria have been extensively studied for their potential in heavy-metal bioremediation. However, the occurrence of elevated nitrate in contaminated environments has been shown to inhibit sulfate reduction activity. Although the inhibition has been suggested to result from the competition with nitrate-reducing bacteria, the possibility of direct inhibition of sulfate reducers by elevated nitrate needs to be explored. Using Desulfovibrio vulgaris as a model sulfate-reducing bacterium, functional genomics analysis reveals that osmotic stress contributed to growth inhibition by nitrate as shown by the upregulation of the glycine/betaine transporter genes and the relief of nitrate inhibition by osmoprotectants. The observation that significant growth inhibition was effected by 70 mM NaNO{sub 3} but not by 70 mM NaCl suggests the presence of inhibitory mechanisms in addition to osmotic stress. The differential expression of genes characteristic of nitrite stress responses, such as the hybrid cluster protein gene, under nitrate stress condition further indicates that nitrate stress response by D. vulgaris was linked to components of both osmotic and nitrite stress responses. The involvement of the oxidative stress response pathway, however, might be the result of a more general stress response. Given the low similarities between the response profiles to nitrate and other stresses, less-defined stress response pathways could also be important in nitrate stress, which might involve the shift in energy metabolism. The involvement of nitrite stress response upon exposure to nitrate may provide detoxification mechanisms for nitrite, which is inhibitory to sulfate-reducing bacteria, produced by microbial nitrate reduction as a metabolic intermediate and may enhance the survival of sulfate-reducing bacteria in environments with elevated nitrate level.

  12. Factors affecting the recovery of bacteria in freeze-dried model systems

    E-Print Network [OSTI]

    Custer, Carl Steven

    1970-01-01T23:59:59.000Z

    cellulose, and subsequently freeze-dried. The influences of the rate of freezing, time and atmosphere of storage, position of the bacteria within the model system and condition of incubation upon the survival and metabolic injury of the freeze-dried... storage. The type of atmosphere in which freeze-dried preparations were stored was important to bacterial survival. Nitrogen was less damaging to freeze-dried cells than air. Strata studies indicated that the viable bacterial population density...

  13. Effects of UV Light Disinfection on Tetracycline Resistant Bacteria in Wastewater Effluents

    E-Print Network [OSTI]

    Childress, Hannah

    2011-10-21T23:59:59.000Z

    and support. I would also like to thank Bailey Sullivan for teaching lab procedures, and the operators at the wastewater treatment plant for their assistance. I am grateful to the Department of Biological and Agricultural Engineering for providing a... of the requirements for the degree of MASTER OF SCIENCE August 2010 Major Subject: Biological and Agricultural Engineering EFFECTS OF UV LIGHT DISINFECTION ON TETRACYCLINE RESISTANT BACTERIA IN WASTEWATER EFFLUENTS A Thesis by HANNAH...

  14. Ameliorating risk: Culturable and metagenomic monitoring of the 14 year decline of a genetically engineered microorganism at a bioremediation field site

    SciTech Connect (OSTI)

    Layton, Alice [University of Tennessee, Knoxville (UTK); Smart, Abby E. [University of Tennessee, Knoxville (UTK); Chauhan, Archana [University of Tennessee, Knoxville (UTK); Ripp, Steven Anthony [University of Tennessee, Knoxville (UTK); Williams, Daniel [University of Tennessee, Knoxville (UTK); Burton, Whitney [University of Tennessee, Knoxville (UTK); Moser, Scott [University of Tennessee, Knoxville (UTK); Phillips, Jana Randolph [ORNL; Palumbo, Anthony Vito [ORNL; Sayler, Gary [University of Tennessee, Knoxville (UTK)

    2012-01-01T23:59:59.000Z

    Pseudomonas fluorescens HK44 represented the first genetically engineered microorganism to be approved in the United States for field release for applications related to subsurface soil bioremediation. In October 1996, strain HK44 was introduced into a replicated semi-contained array of soil lysimeters where its luciferase (luxCDABE)-based bioluminescent response to soil-borne polycyclic aromatic hydrocarbon (PAH) contaminants was detected and monitored for the next two years. At the termination of this experiment, it was decided that the lysimeters remain available for future longer-term monitoring efforts, and were thus covered and left essentially undisturbed until the initiation of a large sampling event in 2010, fourteen years after the original release. Although after extensive sampling culturable HK44 cells were not found, additional molecular and metagenomic analyses indicated that genetic signatures of HK44 cells still persisted, with genes diagnostic for the bioluminescent transposon carried by strain HK44 (luxA and tetA) being found at low concentrations (< 5000 copies/g).

  15. Experimental test of whether electrostatically charged micro-organisms and their spores contribute to the onset of arcs across vacuum gaps

    SciTech Connect (OSTI)

    Grisham, L. R.; Halle, A. von; Carpe, A. F.; Gilton, K. R.; Rossi, Guy; Stevenson, T. N. [Princeton University Plasma Physics Laboratory, P. O. Box 451, Princeton, New Jersey 08543 (United States)] [Princeton University Plasma Physics Laboratory, P. O. Box 451, Princeton, New Jersey 08543 (United States)

    2013-12-15T23:59:59.000Z

    Recently it was proposed [L. R. Grisham et al. Phys. Plasmas 19, 023107 (2012)] that one of the initiators of vacuum voltage breakdown between conducting electrodes might be micro-organisms and their spores, previously deposited during exposure to air, which then become electrostatically charged when an electric potential is applied across the vacuum gap. This note describes a simple experiment to compare the number of voltage-conditioning pulses required to reach the nominal maximum operating voltage across a gap between two metallic conductors in a vacuum, comparing cases in which biological cleaning was done just prior to pump-down with cases where this was not done, with each case preceded by exposure to ambient air for three days. Based upon these results, it does not appear that air-deposited microbes and their spores constitute a major pathway for arc initiation, at least for exposure periods of a few days, and for vacuum gaps of a few millimeters, in the regime where voltage holding is usually observed to vary linearly with gap distance.

  16. Experimental Test Of Whether Electrostatically Charged Micro-organisms And Their Spores Contribute To The Onset Of Arcs Across Vacuum Gaps

    SciTech Connect (OSTI)

    none,; Grisham, Larry R.

    2014-02-24T23:59:59.000Z

    Recently it was proposed [L.R. Grisham, A. vonHalle, A.F. Carpe, Guy Rossi, K.R. Gilton, E.D. McBride, E.P. Gilson, A. Stepanov, T.N. Stevenson, Physics of Plasma 19 023107 (2012)] that one of the initiators of vacuum voltage breakdown between condu cting electrodes might be micro-organisms and their spores, previously deposited during exposure to air, which tnen become electrostatically charged when an electric potential is applied across the vacuum gap. The note describes a simple experiment to compare the number of voltage-conditioning pulses required to reach the nominal maxium operating voltage across a gap between two metallic conductors in a vacuum, comparing cases in which biological cleaning was done just prior to pump-down with cases where this was not done, with each preceded by exposure to ambient air for three days. Based upon these results, it does not appear that air-deposited microbes and their spores constitute a major pathway for arc initiation, at least for exposure periods of a few days, and for vacuum gaps of a few millimeters, in the regime where voltage holding is usually observed to vary linearly with gap distance

  17. R E S E A R C H A R T I C L E Free-living and aggregate-associated Planctomycetes in the

    E-Print Network [OSTI]

    and wastewater treatment plants (Schmid et al., 2007). Anam- mox bacteria have an unusual internal compartment

  18. HOUSING/LIVING ARRANGEMENT CHANGE DO NOT USE THIS FORM TO CHANGE YOUR ADDRESS.

    E-Print Network [OSTI]

    Amin, S. Massoud

    HOUSING/LIVING ARRANGEMENT CHANGE DO NOT USE THIS FORM TO CHANGE YOUR ADDRESS. Go online to onestop, select the appropriate housing option. If you live in off-campus housing (without parents), you must have, state, ZIP code, country) PART 2. Housing Information Please change my housing/living arrangements

  19. Housing and Residential Life Rate Sheet Living Learning Communities & Special Interest Housing

    E-Print Network [OSTI]

    Veiga, Pedro Manuel Barbosa

    Housing and Residential Life Rate Sheet Living Learning Communities & Special Interest Housing Housing and Residential Life is proud to offer many Living Learning Communities (LLC) and special interest housing on campus. These communities provide a great opportunity to live with others who share your

  20. Housing and Residential Life Rate Sheet Living Learning Communities & Special Interest Housing

    E-Print Network [OSTI]

    Veiga, Pedro Manuel Barbosa

    Housing and Residential Life Rate Sheet Living Learning Communities & Special Interest Housing Housing and Residential Life is proud to offer many Living-Learning Communities (LLC) and special interest housing on campus. These communities provide a great opportunity to live with others who share your

  1. Reproductive Life Events in the Population Living in the Vicinity of a Nuclear Waste Reprocessing Plant

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Reproductive Life Events in the Population Living in the Vicinity of a Nuclear Waste Reprocessing: There is concern about the health of populations living close to nuclear waste reprocessing plants. We conducted a comparative study on reproductive life events in the general population living near the nuclear waste

  2. The Living Site and Infrastructure Challenge In pursuit of true sustainability in the built environment

    E-Print Network [OSTI]

    Zaferatos, Nicholas C.

    The Living Site and Infrastructure Challenge In pursuit of true sustainability in the built environment Draft Version 1.0 November 2007 #12;NOTIFICATION The Living Site and Infrastructure Challenge - copyright 2007 - Cascadia Region Green Building Council, all rights reserved. The Living Site

  3. Intelligent Transportation Systems: Saving Lives, Time and Money Portland State University

    E-Print Network [OSTI]

    Bertini, Robert L.

    Intelligent Transportation Systems: Saving Lives, Time and Money Portland State University Oct 14, 2009 #12;Intelligent Transportation Systems: Saving Lives, Time and Money James Whiteneck #12;Intelligent Transportation Systems: Saving Lives, Time and Money Portal archives a large amount of data Over

  4. 1Intelligent Transportation Systems: Saving Lives, Time and Money PORTAL Advisory Committee

    E-Print Network [OSTI]

    Bertini, Robert L.

    1Intelligent Transportation Systems: Saving Lives, Time and Money PORTAL Advisory Committee Initial Meeting January 22, 2009 #12;2Intelligent Transportation Systems: Saving Lives, Time and Money Agenda 9:00 Adjourn #12;3Intelligent Transportation Systems: Saving Lives, Time and Money What's in the PORTAL

  5. Diffusive transport without detailed balance in motile bacteria: Does microbiology need statistical physics?

    E-Print Network [OSTI]

    Cates, M E

    2012-01-01T23:59:59.000Z

    Microbiology is the science of microbes, particularly bacteria. Many bacteria are motile: they are capable of self-propulsion. Among these, a significant class execute so-called run-and-tumble motion: they follow a fairly straight path for a certain distance, then abruptly change direction before repeating the process. This dynamics has something in common with Brownian motion (it is diffusive at large scales), and also something in contrast. Specifically, motility parameters such as the run speed and tumble rate depend on the local environment and hence can vary in space. When they do so, even if a steady state is reached, this is not generally invariant under time-reversal: the principle of detailed balance, which restores the microscopic time-reversal symmetry of systems in thermal equilibrium, is mesoscopically absent in motile bacteria. This lack of detailed balance (allowed by the flux of chemical energy that drives motility) creates pitfalls for the unwary modeller. Here I review some statistical mecha...

  6. Diffusive transport without detailed balance in motile bacteria: Does microbiology need statistical physics?

    E-Print Network [OSTI]

    M. E. Cates

    2012-08-20T23:59:59.000Z

    Microbiology is the science of microbes, particularly bacteria. Many bacteria are motile: they are capable of self-propulsion. Among these, a significant class execute so-called run-and-tumble motion: they follow a fairly straight path for a certain distance, then abruptly change direction before repeating the process. This dynamics has something in common with Brownian motion (it is diffusive at large scales), and also something in contrast. Specifically, motility parameters such as the run speed and tumble rate depend on the local environment and hence can vary in space. When they do so, even if a steady state is reached, this is not generally invariant under time-reversal: the principle of detailed balance, which restores the microscopic time-reversal symmetry of systems in thermal equilibrium, is mesoscopically absent in motile bacteria. This lack of detailed balance (allowed by the flux of chemical energy that drives motility) creates pitfalls for the unwary modeller. Here I review some statistical mechanical models for bacterial motility, presenting them as a paradigm for exploring diffusion without detailed balance. I also discuss the extent to which statistical physics is useful in understanding real or potential microbiological experiments.

  7. Isolation of butyrate-utilizing bacteria from thermophilic and mesophilic methane-producing ecosystems

    SciTech Connect (OSTI)

    Henson, J.M.

    1983-01-01T23:59:59.000Z

    The ability of various ecosystems to convert butyrate to methane was studied in order to isolate the bacteria responsible for the conversion. When thermophilic digester sludge was enriched with butyrate, methane was produced without a lag period. Marine sediments enriched with butyrate required a 2-week incubation period before methanogenesis began. A thermophilic digester was studied in more detail and found by most-probable-number enumeration to have ca. 5 x 10/sup 6/ butyrate-utilizing bactera/ml of sludge. A thermophilic butyrate-utilizing bacterium was isolated in coculture with Methanobacterium thermoautotrophicum and a Methanosarcina sp. This bacterium was a gram-negative, slightly curved rod that occurred singly, was nonmotile, and did not appear to produce spores. The thermophilic digester was infused with butyrate at the rate of 10 ..mu..moles/ml of sludge per day. Biogas production increased by 150%, with the percentage of methane increasing from 58% to 68%. Acetate, propionate, and butyrate did not accumulate. Butyrate-utilizing enrichments from mesophilic ecosystems were used in obtaining cocultures of butyrate-utilizing bacteria. These cocultures served as inocula for attempts to isolate pure cultures of butyrate-utilizing bacteria by use of hydrogenase-containing membrane fragments of Escherichia coli. After a 3-week incubation period, colonies appeared only in inoculated tubes that contained membrane fragments and butyrate.

  8. active root-inhabiting microbes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    after solvents. Indigenous microorganisms were stimulated to degrade trichloroethylene (TCE), tetrachloroethylene to stimulate indigenous bacteria (methanotrophs) to degrade...

  9. Liquid Fuel From Bacteria: Engineering Ralstonia eutropha for Production of Isobutanol (IBT) Motor Fuel from CO2, Hydrogen, and Oxygen

    SciTech Connect (OSTI)

    None

    2010-07-15T23:59:59.000Z

    Electrofuels Project: MIT is using solar-derived hydrogen and common soil bacteria called Ralstonia eutropha to turn carbon dioxide (CO2) directly into biofuel. This bacteria already has the natural ability to use hydrogen and CO2 for growth. MIT is engineering the bacteria to use hydrogen to convert CO2 directly into liquid transportation fuels. Hydrogen is a flammable gas, so the MIT team is building an innovative reactor system that will safely house the bacteria and gas mixture during the fuel-creation process. The system will pump in precise mixtures of hydrogen, oxygen, and CO2, and the online fuel-recovery system will continuously capture and remove the biofuel product.

  10. Sources of Nutrients and Fecal Indicator Bacteria to Nearshore Waters on the North Shore of Kaua`i

    E-Print Network [OSTI]

    Paytan, Adina

    indicator bacteria (FIB, i.e., enterococci and Escherichia coli) in nearshore waters and spurred concern :J. H. Street :A. Paytan Institute of Marine Sciences, University of California, Santa Cruz, CA 95064

  11. Bacteria in Ballast Water: The Shipping Industry's Contributions to the Transport and Distribution of Microbial Species in Texas

    E-Print Network [OSTI]

    Neyland, Elizabeth B.

    2010-10-12T23:59:59.000Z

    The transportation of organisms in the ballast water of cargo ships has been recognized as a source of invasive species despite current control measures. Pathogenic bacteria in the ballast tank have been studied but the total diversity...

  12. Reprogramming Bacteria to Seek and Destroy Small Molecules (JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)

    SciTech Connect (OSTI)

    Gallivan, Justin [Emory University] [Emory University

    2012-03-21T23:59:59.000Z

    Justin Gallivan, of Emory University presents a talk titled "Reprogramming Bacteria to Seek and Destroy Small Molecules" at the JGI User 7th Annual Genomics of Energy & Environment Meeting on March 21, 2012 in Walnut Creek, Calif

  13. Functional characterization of flavobacteria rhodopsins reveals a unique class of light-driven chloride pump in bacteria

    E-Print Network [OSTI]

    Yoshizawa, Susumu

    Light-activated, ion-pumping rhodopsins are broadly distributed among many different bacteria and archaea inhabiting the photic zone of aquatic environments. Bacterial proton- or sodium-translocating rhodopsins can convert ...

  14. www.sciencemag.org SCIENCE VOL 341 23 AUGUST 2013 833 BACTERIA MAY NOT ELICIT MUCH SYMPA-

    E-Print Network [OSTI]

    . thermophilus breaks down the milk sugar lactose into tangy lactic acid. But certain viruses--bacterio- phages tar- geted genes in human cells, mice, rats, zebra- fish, bacteria, fruit flies, yeast, nematodes

  15. Reprogramming Bacteria to Seek and Destroy Small Molecules (JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)

    ScienceCinema (OSTI)

    Gallivan, Justin [Emory University

    2013-01-22T23:59:59.000Z

    Justin Gallivan, of Emory University presents a talk titled "Reprogramming Bacteria to Seek and Destroy Small Molecules" at the JGI User 7th Annual Genomics of Energy & Environment Meeting on March 21, 2012 in Walnut Creek, Calif

  16. Bacteria in Ballast Water: The Shipping Industry's Contributions to the Transport and Distribution of Microbial Species in Texas 

    E-Print Network [OSTI]

    Neyland, Elizabeth B.

    2010-10-12T23:59:59.000Z

    The transportation of organisms in the ballast water of cargo ships has been recognized as a source of invasive species despite current control measures. Pathogenic bacteria in the ballast tank have been studied but the total diversity...

  17. Occurrence, Prevalence, and Disinfection Potential of Tetracycline Resistance Genes and Tetracycline Resistant Bacteria in a Subtropical Watershed 

    E-Print Network [OSTI]

    Sullivan, Bailey Ann

    2013-02-26T23:59:59.000Z

    . The objective of this dissertation research was to gain a better understanding of anthropogenic influences on occurrence of tetracycline resistance and use of traditional disinfection methods for the reduction of tetracycline resistant bacteria and genes...

  18. Production of short lived radioactive beams of radium

    E-Print Network [OSTI]

    Shidling, P D; van der Hoek, D J; Jungmann, K; Kruithof, W; Onderwater, C J G; Sohani, M; Versolato, O O; Willmann, L; Wilschut, H W

    2009-01-01T23:59:59.000Z

    Short lived $^{212,213,214}$Ra isotopes have been produced at the TRI$\\mu$P facility in inverse kinematics via the fusion-evaporation reaction $^{206}$Pb+$^{12}$C at 8 MeV/u. Isotopes are separated from other reaction products online using the TRI$\\mu$P magnetic separator. The energetic radium (Ra) isotopes at the exit of the separator were converted into low energy ions with a thermal ionizer. Ra isotopes have been identified by observing their $\\alpha$ decay and life times.

  19. Production of short lived radioactive beams of radium

    E-Print Network [OSTI]

    P. D. Shidling; G. S. Giri; D. J. van der Hoek; K. Jungmann; W. Kruithof; C. J. G. Onderwater; M. Sohani; O. O. Versolato; L. Willmann; H. W. Wilschut

    2009-02-25T23:59:59.000Z

    Short lived $^{212,213,214}$Ra isotopes have been produced at the TRI$\\mu$P facility in inverse kinematics via the fusion-evaporation reaction $^{206}$Pb+$^{12}$C at 8 MeV/u. Isotopes are separated from other reaction products online using the TRI$\\mu$P magnetic separator. The energetic radium (Ra) isotopes at the exit of the separator were converted into low energy ions with a thermal ionizer. Ra isotopes have been identified by observing their $\\alpha$ decay and life times.

  20. Probing the membrane potential of living cells by dielectric spectroscopy

    E-Print Network [OSTI]

    Corina Bot; Camelia Prodan

    2008-12-17T23:59:59.000Z

    In this paper we demonstrate a quantitative way to measure the membrane potential of live cells by dielectric spectroscopy. We also show that the values of the membrane potential obtained using our technique are in good agreement with those obtained using traditional methods-voltage sensitive dyes. The membrane potential is determined by fitting the experimental dielectric dispersion curves with the dispersion curves obtain from a theoretical model. Variations in the membrane potential were induced by modifying the concentration of potassium chloride in the solution of the cell suspension in the presence of valinomycin. For exemplification of the method, E. coli were chosen for our experiments.

  1. Living well, doing good | Y-12 National Security Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOnItemResearch > The EnergyCenter (LMI-EFRC) - CenterLinks BerkeleyLiving well, doing

  2. Live Oak County, Texas: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer Plant Jump to:Landowners and WindLightingLinthicum,Little ValleyLiuzhou Xinneng BiomassLive

  3. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over OurThe Iron Spin Transition in2, 2003Tool ofTopo II:7.1Tracking Living Cells as They

  4. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over OurThe Iron Spin Transition in2, 2003Tool ofTopo II:7.1Tracking Living Cells as

  5. How Will We Live Forever? | GE Global Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) EnvironmentalGyroSolé(tm) HarmonicbetandEnergy 2010 ABringofExplore Earth'sLive

  6. Live Discussion on Energy 101: Fuel Cells | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC)Integrated Codes |Is Your HomeLatestCenter (LMI-EFRC) -ChoicesListLive Discussion on

  7. Live webcast on groundbreaking results of Algal Biofuels Consortium, June

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC)Integrated Codes |Is Your HomeLatestCenter (LMI-EFRC) -ChoicesListLive

  8. JV between KP Renewables and Living Buildings | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtelInterias Solar EnergyEnergy Information Bar LMorgan JumpLiving

  9. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa del SolStrengthening a solidSynthesisAppliances » TopTours SignTracking Living Cells

  10. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa del SolStrengthening a solidSynthesisAppliances » TopTours SignTracking Living

  11. "Each and everyone of us can make changes in the way we live our lives and become part of the solution [to climate change]"

    E-Print Network [OSTI]

    Scharfstein, Daniel

    Green Guide "Id put my money on the sun and solar energy. What a source of power! I hope we dont have of the solution [to climate change]" Al Gore, ,,An Inconvenient Truth BaltimoreGreen Living Guide www.jhsph.edu/green content useful. Cheers, and happy green living! "Be the change you want to see in the world" -- Gandhi

  12. MOLECULAR APPROACHES FOR IN SITU IDENTIFCIATION OF NITRATE UTILIZATION BY MARINE BACTERIA AND PHYTOPLANKTON

    SciTech Connect (OSTI)

    Frischer, Marc E. [Skidaway Institute of Oceanography; Verity, Peter G.; Gilligan, Mathew R.; Bronk, Deborah A.; Zehr, Jonathan P.; Booth, Melissa G.

    2013-09-12T23:59:59.000Z

    Traditionally, the importance of inorganic nitrogen (N) for the nutrition and growth of marine phytoplankton has been recognized, while inorganic N utilization by bacteria has received less attention. Likewise, organic N has been thought to be important for heterotrophic organisms but not for phytoplankton. However, accumulating evidence suggests that bacteria compete with phytoplankton for nitrate (NO3-) and other N species. The consequences of this competition may have a profound effect on the flux of N, and therefore carbon (C), in ocean margins. Because it has been difficult to differentiate between N uptake by heterotrophic bacterioplankton versus autotrophic phytoplankton, the processes that control N utilization, and the consequences of these competitive interactions, have traditionally been difficult to study. Significant bacterial utilization of DIN may have a profound effect on the flux of N and C in the water column because sinks for dissolved N that do not incorporate inorganic C represent mechanisms that reduce the atmospheric CO2 drawdown via the ?biological pump? and limit the flux of POC from the euphotic zone. This project was active over the period of 1998-2007 with support from the DOE Biotechnology Investigations ? Ocean Margins Program (BI-OMP). Over this period we developed a tool kit of molecular methods (PCR, RT-PCR, Q-PCR, QRT-PCR, and TRFLP) and combined isotope mass spectrometry and flow-cytometric approaches that allow selective isolation, characterization, and study of the diversity and genetic expression (mRNA) of the structural gene responsible for the assimilation of NO3- by heterotrophic bacteria (nasA). As a result of these studies we discovered that bacteria capable of assimilating NO3- are ubiquitous in marine waters, that the nasA gene is expressed in these environments, that heterotrophic bacteria can account for a significant fraction of total DIN uptake in different ocean margin systems, that the expression of nasA is differentially regulated in genetically distinct NO3- assimilating bacteria, and that the best predictors of nasA gene expression are either NO3- concentration or NO3- uptake rates. These studies provide convincing evidence of the importance of bacterial utilization of NO3-, insight into controlling processes, and provide a rich dataset that are being used to develop linked C and N modeling components necessary to evaluate the significance of bacterial DIN utilization to global C cycling. Furthermore, as a result of BI-OMP funding we made exciting strides towards institutionalizing a research and education based collaboration between the Skidaway Institute of Oceanography (SkIO) and Savannah State University (SSU), an historically black university within the University System of Georgia with undergraduate and now graduate programs in marine science. The BI-OMP program, in addition to supporting undergraduate (24) graduate (10) and postdoctoral (2) students, contributed to the development of a new graduate program in Marine Sciences at SSU that remains an important legacy of this project. The long-term goals of these collaborations are to increase the capacity for marine biotechnology research and to increase representation of minorities in marine, environmental and biotechnological sciences.

  13. Exploration of Simple Analytical Approaches for Rapid Detection of Pathogenic Bacteria

    SciTech Connect (OSTI)

    Salma Rahman

    2005-12-17T23:59:59.000Z

    Many of the current methods for pathogenic bacterial detection require long sample-preparation and analysis time, as well as complex instrumentation. This dissertation explores simple analytical approaches (e.g., flow cytometry and diffuse reflectance spectroscopy) that may be applied towards ideal requirements of a microbial detection system, through method and instrumentation development, and by the creation and characterization of immunosensing platforms. This dissertation is organized into six sections. In the general Introduction section a literature review on several of the key aspects of this work is presented. First, different approaches for detection of pathogenic bacteria will be reviewed, with a comparison of the relative strengths and weaknesses of each approach, A general overview regarding diffuse reflectance spectroscopy is then presented. Next, the structure and function of self-assembled monolayers (SAMs) formed from organosulfur molecules at gold and micrometer and sub-micrometer patterning of biomolecules using SAMs will be discussed. This section is followed by four research chapters, presented as separate manuscripts. Chapter 1 describes the efforts and challenges towards the creation of imunosensing platforms that exploit the flexibility and structural stability of SAMs of thiols at gold. 1H, 1H, 2H, 2H-perfluorodecyl-1-thiol SAM (PFDT) and dithio-bis(succinimidyl propionate)-(DSP)-derived SAMs were used to construct the platform. Chapter 2 describes the characterization of the PFDT- and DSP-derived SAMs, and the architectures formed when it is coupled to antibodies as well as target bacteria. These studies used infrared reflection spectroscopy (IRS), X-ray photoelectron spectroscopy (XPS), and electrochemical quartz crystal microbalance (EQCM), Chapter 3 presents a new sensitive, and portable diffuse reflection based technique for the rapid identification and quantification of pathogenic bacteria. Chapter 4 reports research efforts in the construction and evaluation of a prototype flow cytometry based cell detector and enumerator. This final research chapter is followed by a general summation and future prospectus section that concludes this dissertation.

  14. Soft inertial microfluidics for high throughput separation of bacteria from human blood cells

    SciTech Connect (OSTI)

    Wu, Zhigang; Willing, Ben; Bjerketorp, Joakim; Jansson, Janet K.; Hjort, Klas

    2009-01-05T23:59:59.000Z

    We developed a new approach to separate bacteria from human blood cells based on soft inertial force induced migration with flow defined curved and focused sample flow inside a microfluidic device. This approach relies on a combination of an asymmetrical sheath flow and proper channel geometry to generate a soft inertial force on the sample fluid in the curved and focused sample flow segment to deflect larger particles away while the smaller ones are kept on or near the original flow streamline. The curved and focused sample flow and inertial effect were visualized and verified using a fluorescent dye primed in the device. First the particle behavior was studied in detail using 9.9 and 1.0 {micro}m particles with a polymer-based prototype. The prototype device is compact with an active size of 3 mm{sup 2}. The soft inertial effect and deflection distance were proportional to the fluid Reynolds number (Re) and particle Reynolds number (Re{sub p}), respectively. We successfully demonstrated separation of bacteria (Escherichia coli) from human red blood cells at high cell concentrations (above 10{sup 8}/mL), using a sample flow rate of up to 18 {micro}L/min. This resulted in at least a 300-fold enrichment of bacteria at a wide range of flow rates with a controlled flow spreading. The separated cells were proven to be viable. Proteins from fractions before and after cell separation were analyzed by gel electrophoresis and staining to verify the removal of red blood cell proteins from the bacterial cell fraction. This novel microfluidic process is robust, reproducible, simple to perform, and has a high throughput compared to other cell sorting systems. Microfluidic systems based on these principles could easily be manufactured for clinical laboratory and biomedical applications.

  15. Production of extracellular nucleic acids by genetically altered bacteria in aquatic-environment microcosms

    SciTech Connect (OSTI)

    Paul, J.H.; David, A.W.

    1989-01-01T23:59:59.000Z

    Factors which affect the production of extracellular DNA by genetically altered strains of Escherichia coli, Pseudomonas aeruginosa, Pseudomonas cepacia, and Bradyrhizobium japonicum in aquatic environments were investigated. The presence or absence of the ambient microbial community had little effect on the production of extracellular DNA. Results indicate the extracellular-DNA production by genetically altered bacteria released into aquatic environments is more strongly influenced by physiochemical factors than biotic factors; extracellular-DNA production rates are usually greater for organisms released in freshwater than marine environments; and ambient microbial populations can readily utilize materials released by these organisms.

  16. Light-harvesting in bacteria exploits a critical interplay between transport and trapping dynamics

    E-Print Network [OSTI]

    Felipe Caycedo-Soler; Ferney J. Rodriguez; Luis Quiroga; Neil F. Johnson

    2010-03-11T23:59:59.000Z

    Light-harvesting bacteria Rhodospirillum Photometricum were recently found to adopt strikingly different architectures depending on illumination conditions. We present analytic and numerical calculations which explain this observation by quantifying a dynamical interplay between excitation transfer kinetics and reaction center cycling. High light-intensity membranes (HLIM) exploit dissipation as a photo-protective mechanism, thereby safeguarding a steady supply of chemical energy, while low light-intensity membranes (LLIM) efficiently process unused illumination intensity by channelling it to open reaction centers. More generally, our analysis elucidates and quantifies the trade-offs in natural network design for solar energy conversion.

  17. A technique for quantitative and qualitative viewing of aquatic bacteria using scanning electron microscopy 

    E-Print Network [OSTI]

    Dreier, Thomas Michael

    1977-01-01T23:59:59.000Z

    microscopic enumeration techniques. Water samples are concentrated on pre-wetted (Triton X-100) Nuclepore filters (0. 2 um pore size) to prov1de a uniform distri- bution of bacteria on the filter surface and vacuum filtered (660 Torr). The filter... is transferred to a petri dish containing filter paper soaked 1n 2% glutaraldehyde and the bacter1a are fixed for one hour. Dehydration 1s performed by transferr1ng the filters through a series of petri dishes conta1ning filter paper saturated with 25, 50, 75...

  18. A technique for quantitative and qualitative viewing of aquatic bacteria using scanning electron microscopy

    E-Print Network [OSTI]

    Dreier, Thomas Michael

    1977-01-01T23:59:59.000Z

    microscopic enumeration techniques. Water samples are concentrated on pre-wetted (Triton X-100) Nuclepore filters (0. 2 um pore size) to prov1de a uniform distri- bution of bacteria on the filter surface and vacuum filtered (660 Torr). The filter... is transferred to a petri dish containing filter paper soaked 1n 2% glutaraldehyde and the bacter1a are fixed for one hour. Dehydration 1s performed by transferr1ng the filters through a series of petri dishes conta1ning filter paper saturated with 25, 50, 75...

  19. Magnetic resonance imaging of living systems by remote detection

    DOE Patents [OSTI]

    Wemmer, David; Pines, Alexander; Bouchard, Louis; Xu, Shoujun; Harel, Elad; Budker, Dmitry; Lowery, Thomas; Ledbetter, Micah

    2013-10-29T23:59:59.000Z

    A novel approach to magnetic resonance imaging is disclosed. Blood flowing through a living system is prepolarized, and then encoded. The polarization can be achieved using permanent or superconducting magnets. The polarization may be carried out upstream of the region to be encoded or at the place of encoding. In the case of an MRI of a brain, polarization of flowing blood can be effected by placing a magnet over a section of the body such as the heart upstream of the head. Alternatively, polarization and encoding can be effected at the same location. Detection occurs at a remote location, using a separate detection device such as an optical atomic magnetometer, or an inductive Faraday coil. The detector may be placed on the surface of the skin next to a blood vessel such as a jugular vein carrying blood away from the encoded region.

  20. Near-Term Climate Mitigation by Short-Lived Forcers

    SciTech Connect (OSTI)

    Smith, Steven J.; Mizrahi, Andrew H.

    2013-08-12T23:59:59.000Z

    Emissions reductions focused on anthropogenic climate forcing agents with relatively short atmospheric lifetimes such as methane (CH4) and black carbon (BC) have been suggested as a strategy to reduce the rate of climate change over the next several decades. We find that reductions of methane and BC would likely have only a modest impact on near-term climate warming. Even with maximally feasible reductions phased in from 2015 to 2035, global mean temperatures in 2050 are reduced by 0.16 °C, with an uncertainty range of 0.04-0.36°C, with the high end of this range only possible if total historical aerosol forcing is small. More realistic mitigation scenarios would likely provide a smaller climate benefit. The climate benefits from targeted reductions in short-lived forcing agents are smaller than previously estimated and are not substantially different in magnitude from the benefits due to a comprehensive climate policy.

  1. Air Leakage and Air Transfer Between Garage and Living Space

    SciTech Connect (OSTI)

    Rudd, A.

    2014-09-01T23:59:59.000Z

    This research project focused on evaluation of air transfer between the garage and living space in a single-family detached home constructed by a production homebuilder in compliance with the 2009 International Residential Code and the 2009 International Energy Conservation Code. The project gathered important information about the performance of whole-building ventilation systems and garage ventilation systems as they relate to minimizing flow of contaminated air from garage to living space. A series of 25 multi-point fan pressurization tests and additional zone pressure diagnostic testing characterized the garage and house air leakage, the garage-to-house air leakage, and garage and house pressure relationships to each other and to outdoors using automated fan pressurization and pressure monitoring techniques. While the relative characteristics of this house may not represent the entire population of new construction configurations and air tightness levels (house and garage) throughout the country, the technical approach was conservative and should reasonably extend the usefulness of the results to a large spectrum of house configurations from this set of parametric tests in this one house. Based on the results of this testing, the two-step garage-to-house air leakage test protocol described above is recommended where whole-house exhaust ventilation is employed. For houses employing whole-house supply ventilation (positive pressure) or balanced ventilation (same pressure effect as the Baseline condition), adherence to the EPA Indoor airPLUS house-to-garage air sealing requirements should be sufficient to expect little to no garage-to-house air transfer.

  2. Long-lived activation products in reactor materials

    SciTech Connect (OSTI)

    Evans, J.C.; Lepel, E.L.; Sanders, R.W.; Wilkerson, C.L.; Silker, W.; Thomas, C.W.; Abel, K.H.; Robertson, D.R.

    1984-08-01T23:59:59.000Z

    The purpose of this program was to assess the problems posed to reactor decommissioning by long-lived activation products in reactor construction materials. Samples of stainless steel, vessel steel, concrete, and concrete ingredients were analyzed for up to 52 elements in order to develop a data base of activatable major, minor, and trace elements. Large compositional variations were noted for some elements. Cobalt and niobium concentrations in stainless steel, for example, were found to vary by more than an order of magnitude. A thorough evaluation was made of all possible nuclear reactions that could lead to long lived activation products. It was concluded that all major activation products have been satisfactorily accounted for in decommissioning planning studies completed to date. A detailed series of calculations was carried out using average values of the measured compositions of the appropriate materials to predict the levels of activation products expected in reactor internals, vessel walls, and bioshield materials for PWR and BWR geometries. A comparison is made between calculated activation levels and regulatory guidelines for shallow land disposal according to 10 CFR 61. This analysis shows that PWR and BWR shroud material exceeds the Class C limits and is, therefore, generally unsuitable for near-surface disposal. The PWR core barrel material approaches the Class C limits. Most of the remaining massive components qualify as either Class A or B waste with the bioshield clearly Class A, even at the highest point of activation. Selected samples of activated steel and concrete were subjected to a limited radiochemical analysis program as a verification of the computer model. Reasonably good agreement with the calculations was obtained where comparison was possible. In particular, the presence of /sup 94/Nb in activated stainless steel at or somewhat above expected levels was confirmed.

  3. Our academic landscape is rapidly changing. We all live in a digital world. Use of technology infuses every part of our personal and professional lives and our connections are global. Technology has brought us new and

    E-Print Network [OSTI]

    Haase, Markus

    infuses every part of our personal and professional lives and our connections are global. Technology has

  4. Structural similarities between biogenic uraninites produced by phylogenetically and metabolically diverse bacteria.

    SciTech Connect (OSTI)

    Sharp, Jonathan; Schofield, Eleanor J.; Veeramani, Harish; Suvorova, Elena; Kennedy, David W.; Marshall, Matthew J.; Mehta, Apurva; Bargar, John R.; Bernier-Latmani, Rizlan

    2009-11-01T23:59:59.000Z

    While the product of microbial uranium reduction is often reported to be“UO2”, a comprehensive characterization including stoichiometry and unit cell determination is available for only one Shewanella species. Here, we compare the products of batch uranyl reduction by a collection of dissimilatory metal- and sulfate-reducing bacteria of the genera Shewanella, Geobacter, Anaeromyxobacter, and Desulfovibrio under similar laboratory conditions. Our results demonstrate that U(VI) bioreduction by this assortment of commonly studied, environmentally relevant bacteria leads to the precipitation of uraninite with a composition between UO2.00 and UO2.075, regardless of phylogenetic or metabolic diversity. Coupled analyses, including electron microscopy, X-ray absorption spectroscopy, and powder diffraction, confirm that structurally and chemically analogous uraninite solids are produced. These biogenic uraninites have particle diameters of about 2-3 nm and lattice constants consistent with UO2.0 and exhibit a high degree of intermediate-range order. Results indicate that phylogenetic and metabolic variability within delta- and gamma-proteobacteria has little effect on nascent biouraninite structure or crystal size under the investigated conditions.

  5. Chemotactic behavior of deep subsurface bacteria toward carbohydrates, amino acids and a chlorinated alkene

    SciTech Connect (OSTI)

    Lopez de Victoria, G. (Puerto Rico Univ., Rio Piedras (Puerto Rico). Dept. of Biology)

    1989-02-01T23:59:59.000Z

    The chemotactic behavior of deep terrestrial subsurface bacteria toward amino acids, carbohydrates and trichloroethylene was assayed using a modification of the capillary method and bacterial enumeration by acridine orange direct counts. Eleven isolates of bacteria isolated from six different geological formations were investigated. A bimodal response rather than an absolute positive or negative response was observed in most assays. Most of the isolates were positively chemotactic to low concentrations of substrates and were repelled by high concentrations of the same substrate. However, this was not the case for trichloroethylene (TCE) which was mostly an attractant and elicited the highest responses in all the isolates when compared with amino acids and carbohydrates. The movement rates of these isolates in aseptic subsurface sediments in the absence and presence of TCE were also determined using a laboratory model. All of the isolates showed distinct response range, peak, and threshold concentrations when exposed to the same substrates suggesting that they are possibly different species as has been inferred from DNA homology studies. 101 refs., 4 figs., 57 tabs.

  6. Live Webinar on Better Buildings Case Competition: Energy Efficiency in the Restaurant Franchise Model Case Study

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webinar titled "A Side of Savings: Energy Efficiency in the Restaurant Franchise Model Case Study."

  7. Live Webinar on Better Buildings Case Competition: Taking Commercial PACE Financing to Scale, a Case Study

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webinar titled "A Side of Savings: Energy Efficiency in the Restaurant Franchise Model Case Study."

  8. Nuclear $\\beta$-decay half-lives in the relativistic point-coupling model

    E-Print Network [OSTI]

    Wang, Z Y; Niu, Y F; Guo, J Y

    2015-01-01T23:59:59.000Z

    The self-consistent proton-neutron quasiparticle random phase approximation approach is employed to calculate $\\beta$-decay half-lives of neutron-rich even-even nuclei with $8\\leqslant Z \\leqslant 30$. A newly proposed nonlinear point-coupling effective interaction PC-PK1 is used in the calculations. It is found that the isoscalar proton-neutron pairing interaction can significantly reduce $\\beta$-decay half-lives. With an isospin-dependent isoscalar proton-neutron pairing strength, our results well reproduce the experimental $\\beta$-decay half-lives, although the pairing strength is not adjusted using the half-lives calculated in this study.

  9. ORGANIC GEOCHEMICAL STUDIES. II. THE DISTRIBUTION OF ALIPHATIC HYDROCARBONS IN ALGAE, BACTERIA, AND IN A RECENT LAKE SEDIMENT: A PRELIMINARY REPORT

    E-Print Network [OSTI]

    Han, Jerry; McCarthy, E.D.; Van Hoeven Jr., William; Calvin, Melvin; Bradley, W. H.

    2008-01-01T23:59:59.000Z

    significantly to the hydrocarbons of higher molecular weightDISTRIBUTION OF ALIPHATIC HYDROCARBONS IN ALGAE, BACTERIA,T E DISTRIBUTION O ALIPHATIC HYDROCARBONS H F A PRELIMINARY

  10. Vanderbilt University Pet Policy for Live-in Employees Vanderbilt University permits live-in employees, be they faculty or staff, to own and keep pets

    E-Print Network [OSTI]

    Bordenstein, Seth

    1 Vanderbilt University Pet Policy for Live-in Employees Vanderbilt pets in their University assigned housing. The requirements of the policy are as follows: 1. Pets are limited to domesticated dogs, domesticated cats and fish

  11. Memory Saves Lives: Inter-generational Warnings Effectiveness - 13556

    SciTech Connect (OSTI)

    Van Luik, Abraham; Patterson, Russell [U.S. Department of Energy, Carlsbad Field Office, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States)] [U.S. Department of Energy, Carlsbad Field Office, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States); Shafer, David [U.S. Department of Energy, Office of Legacy Management, 11025 Dover Street, Suite 1000, Westminster, CO 80021 (United States)] [U.S. Department of Energy, Office of Legacy Management, 11025 Dover Street, Suite 1000, Westminster, CO 80021 (United States); Klein, Thomas [URS Regulatory and Environmental Services, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States)] [URS Regulatory and Environmental Services, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States)

    2013-07-01T23:59:59.000Z

    The 2011 Tohoku earthquake and tsunami was a world-class natural disaster. It has been described as the most powerful earthquake ever in Japan, and as one of the most powerful earthquakes ever noted in the world. The toll in terms of human lives lost and property destruction was unimaginable. Even the word 'horrible' is inadequate to describe the suffering and misery that resulted. Nations with nuclear power programs are engaged in, or at least planning to become engaged in, arranging to eventually dispose of their higher-level radioactive waste materials in deep geologic repositories. Geologic repositories are passive safety systems, and if undisturbed isolate these dangerous materials form the biosphere for extremely long times. The key words, however, are 'if undisturbed'. To assure that future generations do not inadvertently drill into repositories, national programs, and the international community (the Records, Knowledge and Memory (RK and M) preservation project of the Nuclear Energy Agency, for example), are proposing to place markers and/or monuments on closed repository sites that say 'do not drill here, and this is why' in various sophisticated ways. Such markers or monuments are attempts at providing passive institutional controls. The effectiveness of messages from past generations to a present generation may give an indication of how effective such passive institutional controls may be. (authors)

  12. Forrestal Building Lighting Retrofit Second Live Test Demonstration (LTD)

    SciTech Connect (OSTI)

    Halverson, M.A.; Schmelzer, J.R.; Parker, G.B.

    1993-02-01T23:59:59.000Z

    This report describes and summarizes the Forrestal Building Lighting Retrofit Live Test demonstration (LTD) performed by Pacific Northwest Laboratory (PNL) in Room 5E-080 of the DOE Forrestal Building in Washington, D.C. The purpose of the LTD was to evaluate proposed lighting retrofits for compliance with the requirements laid out in the request for proposal (RFP) for the Shared Energy Savings (SES) Lighting Retrofit Project for the Forrestal Building, Washington, D.C. Testing was conducted from March 9 through March 18, 1992, and again on August 3 through August 6, 1992. Four contractors were initially tested in March. Then, two contractors were retested in August due to changes in the rebate schedule for electronic ballasts being offered by the Potomac Electric Power Company (PEPCO), the utility servicing the Forrestal Building. The two contractors tested in March were retested with different ballasts, tubes, and reflectors. The results from these new tests are reported here and compared with those from the earlier tests.

  13. Evolution of an Early Illness Warning System to Monitor Frail Elders in Independent Living

    E-Print Network [OSTI]

    He, Zhihai "Henry"

    Evolution of an Early Illness Warning System to Monitor Frail Elders in Independent Living Gregory the evolution of an early illness warning system used by an interdisciplinary team composed of clinicians and engineers in an independent living facility. The early illness warning system consists of algorithms which

  14. Adapting the Home for Independent Living for the Elderly and Handicapped.

    E-Print Network [OSTI]

    Young, Sue

    1979-01-01T23:59:59.000Z

    * A feeling of independence is important to everyone, including the elderly and handicapped. The majority of elderly persons in Texas own their homes and wish to live independently as long as health permits. The self worth of the handicapped person... ..... 9 Bedroom ...................................... 10 Adaptation - Neither Expensive nor Difficult .... 10 References .................................... 10 ., ADAPTING THE HOME for independent living for the elderly and handicapped Sue Young...

  15. How did LUCA make a living? Chemiosmosis in the origin of life

    E-Print Network [OSTI]

    Allen, John F.

    necessary for carbon and energy metabolism in all free-living chemotrophs, and presum- ably the first free-living cells too. Proton gradients form naturally at alkaline hydrothermal vents and are viewed as central from the vents. Synthesis of ATP by chemi- osmosis today involves generation of an ion gradient

  16. Tools for Living and Tools for Learning Stefan Carmien and Gerhard Fischer

    E-Print Network [OSTI]

    Fischer, Gerhard

    Tools for Living and Tools for Learning Stefan Carmien and Gerhard Fischer University of Colorado as tools, particularly computational artifacts. We introduce the notions of tools for living and tools for the design of artifacts. The tools concept is then studied in the context of the Memory Aiding Prompting

  17. www.hfhl.umn.edu Healthy Foods, Healthy Lives (HFHL) Institute

    E-Print Network [OSTI]

    Amin, S. Massoud

    Lives (HFHL) Institute is an all-university Institute supported by funds from the University funding cycle (fall 2014) OR proposals may be prepared and submitted to other equivalent Universitywww.hfhl.umn.edu Healthy Foods, Healthy Lives (HFHL) Institute Planning Grant Program for Community-University

  18. From Where You Live to Where You Spend Time: Environmental Contributions to Obesity Risk

    E-Print Network [OSTI]

    Illinois at Chicago, University of

    From Where You Live to Where You Spend Time: Environmental Contributions to Obesity Risk Shannon N contributions to obesity risk Where you live Where you spend time Activity spaces EMA #12;I will mostly can choose from, and we should have that same thing. -Chicago resident #12;Environment and Obesity

  19. Nature Macmillan Publishers Ltd 1997 insulating; and so on. A living polymer-

    E-Print Network [OSTI]

    Nature © Macmillan Publishers Ltd 1997 insulating; and so on. A living polymer- ization method . David A. Tirrell is in the Department of Polymer Science and Engineering, University of Massachusetts. Biopolymers 16, 2005­2028 (1977). 7. Szwarc, M. Carbanions, Living Polymers, and Electron-Transfer Processes

  20. The efficiency of mitochondrial electron transport chain is increased in the long-lived mrg19

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    Babu, M. Madan

    The efficiency of mitochondrial electron transport chain is increased in the long-lived mrg19 mtROS and contribute to longevity. This increased mitochondrial efficiency (i.e. low mtROS generated the observed higher mito- chondrial efficiency in the long-lived mrg19 mutant of Saccharomyces cerevisiae