National Library of Energy BETA

Sample records for bacteria microorganisms living

  1. Microorganism immobilization

    DOE Patents [OSTI]

    Compere, Alicia L.; Griffith, William L.

    1981-01-01

    Live metabolically active microorganisms are immobilized on a solid support by contacting particles of aggregate material with a water dispersible polyelectrolyte such as gelatin, crosslinking the polyelectrolyte by reacting it with a crosslinking agent such as glutaraldehyde to provide a crosslinked coating on the particles of aggregate material, contacting the coated particles with live microorganisms and incubating the microorganisms in contact with the crosslinked coating to provide a coating of metabolically active microorganisms. The immobilized microorganisms have continued growth and reproduction functions.

  2. Method of separating bacteria from free living amoebae

    DOE Patents [OSTI]

    Tyndall, Richard L.

    1994-01-01

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  3. Photosynthetic microorganisms expressing thermostable lipase

    DOE Patents [OSTI]

    Curtiss, Roy; Liu, Xinyao

    2016-04-05

    The present invention encompasses a photosynthetic microorganism that produces biofuels and biofuel precursors.

  4. Bacteria-Mineral Interactions on the Surfaces of Metal-Resistant Bacteria

    SciTech Connect (OSTI)

    Malkin, A J

    2010-03-24

    The extraordinary ability of indigenous microorganisms, like metal-resistant bacteria, for biotransformation of toxic compounds is of considerable interest for the emerging area of environmental bioremediation. However, the underlying mechanisms by which metal-resistant bacteria transform toxic compounds are currently unknown and await elucidation. The project's objective was to study stress-induced responses of metal-resistant bacteria to environmental changes and chemical stimulants. This project involved a multi-institutional collaboration of our LLNL group with the group of Dr. H.-Y. Holman (Lawrence Berkeley National Laboratory). In this project, we have utilized metal-resistant bacteria Arthrobacter oxydans as a model bacterial system. We have utilized atomic force microscopy (AFM) to visualize for the first time at the nanometer scale formation of stress-induced structures on bacterial surfaces in response to Cr (VI) exposure. We have demonstrated that structure, assembly, and composition of these stress-induced structures are dependent on Cr (VI) concentrations. Our AFM observations of the appearance and development of stress-induced layers on the surfaces of Arthrobacter oxydans bacteria exposed to Cr (VI) were confirmed by Dr. Holman's biochemical, electron microscopy, and synchrotron infrared spectromicroscopy studies. In general, in vitro imaging of live microbial and cellular systems represents one of the most challenging issues in application of AFM. Various approaches for immobilization of bacteria on the substrate for in vitro imaging were tested in this project. Imaging of live bacteria was achieved, however further optimization of experimental methods are needed for high-resolution visualization of the cellular environmental structural dynamics by AFM. This project enhanced the current insight into molecular architecture, structural and environmental variability of bacterial systems. The project partially funded research for two book chapters (1

  5. Microorganisms for producing organic acids

    SciTech Connect (OSTI)

    Pfleger, Brian Frederick; Begemann, Matthew Brett

    2014-09-30

    Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.

  6. Proteolysis in hyperthermophilic microorganisms

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ward, Donald E.; Shockley, Keith R.; Chang, Lara S.; Levy, Ryan D.; Michel, Joshua K.; Conners, Shannon B.; Kelly, Robert M.

    2002-01-01

    Proteases are found in every cell, where they recognize and break down unneeded or abnormal polypeptides or peptide-based nutrients within or outside the cell. Genome sequence data can be used to compare proteolytic enzyme inventories of different organisms as they relate to physiological needs for protein modification and hydrolysis. In this review, we exploit genome sequence data to compare hyperthermophilic microorganisms from the euryarchaeotal genus Pyrococcus , the crenarchaeote Sulfolobus solfataricus , and the bacterium Thermotoga maritima . An overview of the proteases in these organisms is given based on those proteases that have been characterized and on putativemore » proteases that have been identified from genomic sequences, but have yet to be characterized. The analysis revealed both similarities and differences in the mechanisms utilized for proteolysis by each of these hyperthermophiles and indicated how these mechanisms relate to proteolysis in less thermophilic cells and organisms.« less

  7. Interplay between microorganisms and geochemistry in geological carbon storage

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Altman, Susan J.; Kirk, Matthew Fletcher; Santillan, Eugenio-Felipe U.; Bennett, Philip C.

    2016-02-28

    Researchers at the Center for Frontiers of Subsurface Energy Security (CFSES) have conducted laboratory and modeling studies to better understand the interplay between microorganisms and geochemistry for geological carbon storage (GCS). We provide evidence of microorganisms adapting to high pressure CO2 conditions and identify factors that may influence survival of cells to CO2 stress. Factors that influenced the ability of cells to survive exposure to high-pressure CO2 in our experiments include mineralogy, the permeability of cell walls and/or membranes, intracellular buffering capacity, and whether cells live planktonically or within biofilm. Column experiments show that, following exposure to acidic water, biomassmore » can remain intact in porous media and continue to alter hydraulic conductivity. Our research also shows that geochemical changes triggered by CO2 injection can alter energy available to populations of subsurface anaerobes and that microbial feedbacks on this effect can influence carbon storage. Our research documents the impact of CO2 on microorganisms and in turn, how subsurface microorganisms can influence GCS. Furthermore, we conclude that microbial presence and activities can have important implications for carbon storage and that microorganisms should not be overlooked in further GCS research.« less

  8. Cellulase producing microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1997-12-30

    Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  9. Cellulase producing microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig

    1997-01-01

    Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  10. Oil Production by a Consortium of Oleaginous Microorganisms grown on primary effluent wastewater

    SciTech Connect (OSTI)

    Hall, Jacqueline; Hetrick, Mary; French, Todd; Hernandez, Rafael; Donaldson, Janet; Mondala, Andro; Holmes, William

    2011-01-01

    Municipal wastewater could be a potential growth medium that has not been considered for cultivating oleaginous microorganisms. This study is designed to determine if a consortium of oleaginous microorganism can successfully compete for carbon and other nutrients with the indigenous microorganisms contained in primary effluent wastewater. RESULTS: The oleaginous consortium inoculated with indigenous microorganisms reached stationary phase within 24 h, reaching a maximum cell concentration of 0.58 g L -1. Water quality post-oleaginous consortium growth reached a maximum chemical oxygen demand (COD) reduction of approximately 81%, supporting the consumption of the glucose within 8 h. The oleaginous consortium increased the amount of oil produced per gram by 13% compared with indigenous microorganisms in raw wastewater. Quantitative polymerase chain reaction (qPCR) results show a substantial population increase in bacteria within the first 24 h when the consortium is inoculated into raw wastewater. This result, along with the fatty acid methyl esters (FAMEs) results, suggests that conditions tested were not sufficient for the oleaginous consortium to compete with the indigenous microorganisms.

  11. Contribution of microorganisms to corrosion

    SciTech Connect (OSTI)

    Thorp, K.E.G.; Crasto, A.S. [Univ. of Dayton Research Inst., OH (United States); Gu, J.D.; Mitchell, R. [Harvard Univ., Cambridge, MA (United States). Div. of Applied Sciences

    1997-08-01

    Current metal primers utilized by the US Air Force contain chromates to inhibit corrosion of the underlying metal. These chromates are both highly toxic and carcinogenic and pose a severe health risk to personnel involved in their application, stripping and disposal. Environmentally-friendly primers with chromate replacements have historically performed poorly with respect to corrosion inhibition. The purpose of this study was to investigate the interaction of chromates with microorganisms in an environment not traditionally associated with biologically-enhanced corrosion to determine if corrosion inhibition by a chromate pigment is, in part, through its action as a biocide. Inoculation of panels which had been coated with a nonchromated primer prior to salt fog exposure and storage in humid conditions resulted in a significant growth of filiform corrosion around a scribe mark. The presence of chromate in the primer severely limited the formation of this corrosion. Likewise, in the absence of the inoculation procedure, the extent of corrosion was strongly diminished. These results suggest that the chromate may be acting as a biocide to limit corrosion which is enhanced by the presence of biological activity.

  12. Global prevalence and distribution of genes and microorganisms...

    Office of Scientific and Technical Information (OSTI)

    Global prevalence and distribution of genes and microorganisms involved in mercury methylation Prev Next Title: Global prevalence and distribution of genes and microorganisms ...

  13. Live Status

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    For Users Live Status Queue Look Classic Queue Look Scheduled Outages Outage Log Science Gateway Status Login Node Status Filesystem Status My NERSC Move to CRT Getting Started...

  14. Degradation of azo dyes by environmental microorganisms and helminths

    SciTech Connect (OSTI)

    Kingthom Chung; Stevens, S.E. Jr. . Dept. of Biology)

    1993-11-01

    The degradation of azo dyes by environmental microorganisms, fungi, and helminths is reviewed. Azo dyes are used in a wide variety of products and can be found in the effluent of most sewage treatment facilities. Substantial quantities of these dyes have been deposited in the environment, particularly in streams and rivers. Azo dyes were shown to affect microbial activities and microbial population sizes in the sediments and in the water columns of aquatic habitats. Only a few aerobic bacteria have been found to reduce azo dyes under aerobic conditions, and little is known about the process. A substantial number of anaerobic bacteria capable of azo dye reduction have been reported. The enzyme responsible for azo dye reduction has been partially purified, and characterization of the enzyme is proceeding. The nematode Ascaris lumbricoides and the cestode Moniezia expanza have been reported to reduce azo dyes anaerobically. Recently the fungus Phanerochaete chrysoporium was reported to mineralize azo dyes via a peroxidation-mediated pathway. A possible degradation pathway for the mineralization of azo dye is proposed and future research needs are discussed.

  15. Bacteria isolated from amoebae/bacteria consortium

    DOE Patents [OSTI]

    Tyndall, R.L.

    1995-05-30

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  16. Bacteria isolated from amoebae/bacteria consortium

    DOE Patents [OSTI]

    Tyndall, Richard L.

    1995-01-01

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  17. Engineered microorganisms having resistance to ionic liquids

    DOE Patents [OSTI]

    Ruegg, Thomas Lawrence; Thelen, Michael P.

    2016-03-22

    The present invention provides for a method of genetically modifying microorganisms to enhance resistance to ionic liquids, host cells genetically modified in accordance with the methods, and methods of using the host cells in a reaction comprising biomass that has been pretreated with ionic liquids.

  18. Recombinant microorganisms for increased production of organic acids

    DOE Patents [OSTI]

    Yi, Jian; Kleff, Susanne; Guettler, Michael V.

    2012-02-21

    Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

  19. Recombinant microorganisms for increased production of organic acids

    DOE Patents [OSTI]

    Yi, Jian; Kleff, Susanne; Guettler, Michael V

    2013-04-30

    Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

  20. Assessment of microorganisms from Indonesian Oil Fields

    SciTech Connect (OSTI)

    Kadarwati, S.; Udiharto, M.; Rahman, M.; Jasjfi, E.; Legowo, E.H.

    1995-12-31

    Petroleum resources have been the mainstay of the national development in Indonesia. However, resources are being depleted after over a century of exploitation, while the demand continues to grow with the rapid economic development of the country. In facing the problem, EOR has been applied in Indonesia, such as the steamflooding project in Duri field, but a more energy efficient technology would be preferable. Therefore, MEOR has been recommended as a promising solution. Our study, aimed at finding indigenous microorganisms which can be developed for application in MEOR, has isolated microbes from some oil fields of Indonesia. These microorganisms have been identified, their activities studied, and the effects of their metabolisms examined. This paper describes the research carried out by LEMIGAS in this respect, giving details on the methods of sampling, incubation, identification, and activation of the microbes as well as tests on the effects of their metabolites, with particular attention to those with potential for application in MEOR.

  1. Consolidated bioprocessing method using thermophilic microorganisms

    DOE Patents [OSTI]

    Mielenz, Jonathan Richard

    2016-02-02

    The present invention is directed to a method of converting biomass to biofuel, and particularly to a consolidated bioprocessing method using a co-culture of thermophilic and extremely thermophilic microorganisms which collectively can ferment the hexose and pentose sugars produced by degradation of cellulose and hemicelluloses at high substrate conversion rates. A culture medium therefor is also provided as well as use of the methods to produce and recover cellulosic ethanol.

  2. Storing data encoded DNA in living organisms

    DOE Patents [OSTI]

    Wong; Pak C. , Wong; Kwong K. , Foote; Harlan P.

    2006-06-06

    Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

  3. Electrosynthesis of Organic Compounds from Carbon Dioxide Is Catalyzed by a Diversity of Acetogenic Microorganisms

    SciTech Connect (OSTI)

    Nevin, KP; Hensley, SA; Franks, AE; Summers, ZM; Ou, JH; Woodard, TL; Snoeyenbos-West, OL; Lovley, DR

    2011-04-20

    Microbial electrosynthesis, a process in which microorganisms use electrons derived from electrodes to reduce carbon dioxide to multicarbon, extracellular organic compounds, is a potential strategy for capturing electrical energy in carbon-carbon bonds of readily stored and easily distributed products, such as transportation fuels. To date, only one organism, the acetogen Sporomusa ovata, has been shown to be capable of electrosynthesis. The purpose of this study was to determine if a wider range of microorganisms is capable of this process. Several other acetogenic bacteria, including two other Sporomusa species, Clostridium ljungdahlii, Clostridium aceticum, and Moorella thermoacetica, consumed current with the production of organic acids. In general acetate was the primary product, but 2-oxobutyrate and formate also were formed, with 2-oxobutyrate being the predominant identified product of electrosynthesis by C. aceticum. S. sphaeroides, C. ljungdahlii, and M. thermoacetica had high (> 80%) efficiencies of electrons consumed and recovered in identified products. The acetogen Acetobacterium woodii was unable to consume current. These results expand the known range of microorganisms capable of electrosynthesis, providing multiple options for the further optimization of this process.

  4. Engineered microorganisms capable of producing target compounds under anaerobic conditions

    DOE Patents [OSTI]

    Buelter, Thomas; Meinhold, Peter; Feldman, Reid M. Renny; Hawkins, Andrew C.; Urano, Jun; Bastian, Sabine; Arnold, Frances

    2012-01-17

    The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

  5. Bioprocessing of lignite coals using reductive microorganisms

    SciTech Connect (OSTI)

    Crawford, D.L.

    1992-03-29

    In order to convert lignite coals into liquid fuels, gases or chemical feedstock, the macromolecular structure of the coal must be broken down into low molecular weight fractions prior to further modification. Our research focused on this aspect of coal bioprocessing. We isolated, characterized and studied the lignite coal-depolymerizing organisms Streptomyces viridosporus T7A, Pseudomonas sp. DLC-62, unidentified bacterial strain DLC-BB2 and Gram-positive Bacillus megaterium strain DLC-21. In this research we showed that these bacteria are able to solubilize and depolymerize lignite coals using a combination of biological mechanisms including the excretion of coal solublizing basic chemical metabolites and extracellular coal depolymerizing enzymes.

  6. RNA polymerase gene, microorganism having said gene and the production of RNA polymerase by the use of said microorganism

    DOE Patents [OSTI]

    Kotani, Hirokazu; Hiraoka, Nobutsugu; Obayashi, Akira

    1991-01-01

    SP6 bacteriophage RNA polymerase is produced by cultivating a new microorganism (particularly new strains of Escherichia coli) harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene and recovering SP6 bacteriophage RNA polymerase from the culture broth. SP6 bacteriophage RNA polymerase gene is provided as are new microorganisms harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene.

  7. Apparatus and method for the desulfurization of petroleum by bacteria

    DOE Patents [OSTI]

    Lizama, H.M.; Scott, T.C.; Scott, C.D.

    1995-10-17

    A method is described for treating petroleum with anaerobic microorganisms acting as biocatalysts that can remove sulfur atoms from hydrocarbon molecules, under anaerobic conditions, and then convert the sulfur atoms to hydrogen sulfide. The microorganisms utilized are from the family known as the ``Sulfate Reducing Bacteria``. These bacteria generate metabolic energy from the oxidation of organic compounds, but use oxidized forms of sulfur as an electron acceptor. Because the biocatalyst is present in the form of bacteria in an aqueous suspension, whereas the reacting substrate consists of hydrocarbon molecules in an organic phase, the actual desulfurization reaction takes place at the aqueous-organic interphase. To ensure adequate interfacial contacting and mass transfer, a biphasic electrostatic bioreactor system is utilized. The bioreactor is utilized to disperse and recoalesce a biocatalyst contained in the aqueous liquid phase into the organic liquid phase containing the sulfur. High-intensity electrical fields rupture the aqueous drops into a plurality of microdroplets and induce continuous coalescence and redispersion as the microdroplets travel through the organic phase, thus increasing surface area. As the aqueous microdroplets progress through the organic phase, the biocatalyst then reacts with the sulfur to produce hydrogen sulfide which is then removed from the bioreactor. The organic liquid, now free of the sulfur, is ready for immediate use or further processing. 5 figs.

  8. Apparatus and method for the desulfurization of petroleum by bacteria

    DOE Patents [OSTI]

    Lizama, Hector M.; Scott, Timothy C.; Scott, Charles D.

    1995-01-01

    A method for treating petroleum with anaerobic microorganisms acting as biocatalysts that can remove sulfur atoms from hydrocarbon molecules, under anaerobic conditions, and then convert the sulfur atoms to hydrogen sulfide. The microorganisms utilized are from the family known as the "Sulfate Reducing Bacteria." These bacteria generate metabolic energy from the oxidation of organic compounds, but use oxidized forms of sulfur as an electron acceptor. Because the biocatalyst is present in the form of bacteria in an aqueous suspension, whereas the reacting substrate consists of hydrocarbon molecules in an organic phase, the actual desulfurization reaction takes place at the aqueous-organic interphase. To ensure adequate interfacial contacting and mass transfer, a biphasic electrostatic bioreactor system is utilized. The bioreactor is utilized to disperse and recoalesce a biocatalyst contained in the aqueous liquid phase into the organic liquid phase containing the sulfur. High-intensity electrical fields rupture the aqueous drops into a plurality of microdroplets and induce continuous coalescence and redispersion as the microdroplets travel through the organic phase, thus increasing surface area. As the aqueous microdroplets progress through the organic phase, the biocatalyst then reacts with the sulfur to produce hydrogen sulfide which is then removed from the bioreactor. The organic liquid, now free of the sulfur, is ready for immediate use or further processing.

  9. Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism

    DOE Patents [OSTI]

    Gaddy, J.L.; Clausen, E.C.

    1992-12-22

    A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H[sub 2]O and/or CO[sub 2] and H[sub 2] in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate. 3 figs.

  10. Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism

    DOE Patents [OSTI]

    Gaddy, James L.; Clausen, Edgar C.

    1992-01-01

    A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H.sub.2 O and/or CO.sub.2 and H.sub.2 in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate.

  11. Living a Sustainable Future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Living a Sustainable Future Living a Sustainable Future August 1, 2013 Biomass to fuel project The Laboratory's biomass team is working to solve the energy crisis through...

  12. Living a Sustainable Future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Living a Sustainable Future Living a Sustainable Future August 1, 2013 Biomass to fuel project The Laboratory's biomass team is working to solve the energy crisis through ...

  13. Community analysis of plant biomass-degrading microorganisms from Obsidian Pool, Yellowstone National Park

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Vishnivetskaya, Tatiana A.; Hamilton-Brehm, Scott D.; Podar, Mircea; Mosher, Jennifer J.; Palumbo, Anthony V.; Phelps, Tommy J.; Keller, Martin; Elkins, James G.

    2014-10-16

    The conversion of lignocellulosic biomass into biofuels can potentially be improved by employing robust microorganisms and enzymes that efficiently deconstruct plant polysaccharides at elevated temperatures. Many of the geothermal features of Yellowstone National Park (YNP) are surrounded by vegetation providing a source of allochthonic material to support heterotrophic microbial communities adapted to utilize plant biomass as a primary carbon and energy source. In this paper, a well-known hot spring environment, Obsidian Pool (OBP), was examined for potential biomass-active microorganisms using cultivation-independent and enrichment techniques. Analysis of 33,684 archaeal and 43,784 bacterial quality-filtered 16S rRNA gene pyrosequences revealed that archaeal diversitymore » in the main pool was higher than bacterial; however, in the vegetated area, overall bacterial diversity was significantly higher. Of notable interest was a flooded depression adjacent to OBP supporting a stand of Juncus tweedyi, a heat-tolerant rush commonly found growing near geothermal features in YNP. The microbial community from heated sediments surrounding the plants was enriched in members of the Firmicutes including potentially (hemi)cellulolytic bacteria from the genera Clostridium, Anaerobacter, Caloramator, Caldicellulosiruptor, and Thermoanaerobacter. Enrichment cultures containing model and real biomass substrates were established at a wide range of temperatures (55–85 °C). Microbial activity was observed up to 80 °C on all substrates including Avicel, xylan, switchgrass, and Populus sp. Finally, independent of substrate, Caloramator was enriched at lower (<65 °C) temperatures while highly active cellulolytic bacteria Caldicellulosiruptor were dominant at high (>65 °C) temperatures.« less

  14. Material to Efficiently and Economically Obtain Microorganism and

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Microalgae - Energy Innovation Portal Biomass and Biofuels Biomass and Biofuels Advanced Materials Advanced Materials Find More Like This Return to Search Material to Efficiently and Economically Obtain Microorganism and Microalgae Ames Laboratory Contact AMES About This Technology Technology Marketing SummaryTechnology provides an economical and efficient process to harvest microorganisms like microalgae from its growth media.Description The interest in using algae as feedstock for biofuel

  15. Microorganisms to Speed Production of Biofuels - Energy Innovation Portal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Microorganisms to Speed Production of Biofuels Oak Ridge National Laboratory Contact ORNL About This Technology Technology Marketing SummaryResearchers at ORNL developed microorganisms that can quickly overcome the resistance of biomass to breakdown, and improved both the cost and efficiency of the biofuel conversion process.DescriptionConventional biomass pretreatment methods release sugars, weak acids, and metabolic by-products that slow down or even stop fermentation, resulting in slower

  16. Microorganisms having enhanced tolerance to inhibitors and stress

    DOE Patents [OSTI]

    Brown, Steven D.; Yang, Shihui

    2014-07-29

    The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.

  17. Microorganisms having enhanced resistance to acetate and methods of use

    DOE Patents [OSTI]

    Brown, Steven D; Yang, Shihui

    2014-10-21

    The present invention provides isolated or genetically modified strains of microorganisms that display enhanced resistance to acetate as a result of increased expression of a sodium proton antiporter. The present invention also provides methods for producing such microbial strains, as well as related promoter sequences and expression vectors. Further, the present invention provides methods of producing alcohol from biomass materials by using microorganisms with enhanced resistance to acetate.

  18. Fuel from Bacteria, CO2, Water, and Solar Energy: Engineering a Bacterial Reverse Fuel Cell

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: Harvard is engineering a self-contained, scalable Electrofuels production system that can directly generate liquid fuels from bacteria, carbon dioxide (CO2), water, and sunlight. Harvard is genetically engineering bacteria called Shewanella, so the bacteria can sit directly on electrical conductors and absorb electrical current. This current, which is powered by solar panels, gives the bacteria the energy they need to process CO2 into liquid fuels. The Harvard team pumps this CO2 into the system, in addition to water and other nutrients needed to grow the bacteria. Harvard is also engineering the bacteria to produce fuel molecules that have properties similar to gasoline or diesel fuelmaking them easier to incorporate into the existing fuel infrastructure. These molecules are designed to spontaneously separate from the water-based culture that the bacteria live in and to be used directly as fuel without further chemical processing once theyre pumped out of the tank.

  19. Bioengineering and Coordination of Regulatory Networks and Intracellular Complexes to Maximize Hydrogen Production by Phototrophic Microorganisms

    SciTech Connect (OSTI)

    Tabita, F. Robert [The Ohio State University] [The Ohio State University

    2013-07-30

    In this study, the Principal Investigator, F.R. Tabita has teemed up with J. C. Liao from UCLA. This project's main goal is to manipulate regulatory networks in phototrophic bacteria to affect and maximize the production of large amounts of hydrogen gas under conditions where wild-type organisms are constrained by inherent regulatory mechanisms from allowing this to occur. Unrestrained production of hydrogen has been achieved and this will allow for the potential utilization of waste materials as a feed stock to support hydrogen production. By further understanding the means by which regulatory networks interact, this study will seek to maximize the ability of currently available unrestrained organisms to produce hydrogen. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Moreover, due to their great metabolic versatility, such organisms highly regulate these processes in the cell and since virtually all such capabilities are dispensable, excellent experimental systems to study aspects of molecular control and biochemistry/physiology are available.

  20. Biofuels from Solar Energy and Bacteria: Electrofuels Via Direct Electron Transfer from Electrodes to Microbes

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: UMass is feeding renewable electricity to bacteria to provide the microorganisms with the energy they need to turn carbon dioxide (CO2) directly into liquid fuels. UMass’ energy-to-fuels conversion process is anticipated to be more efficient than current biofuels approaches in part because this process will leverage the high efficiency of photovoltaics to convert solar energy into electricity. UMass is using bacteria already known to produce biofuel from electric current and CO2 and working to increase the amount of electric current those microorganisms will accept and use for biofuels production. In collaboration with scientists at University of California, San Diego, the UMass team is also investigating the use of hydrogen sulfide as a source of energy to power biofuel production.

  1. Novel microorganism for selective separation of coal from pyrite and ash. Final report

    SciTech Connect (OSTI)

    Misra, M.; Smith, R.W.

    1995-09-01

    The separation of fine coal from ash and pyrite was evaluated using a microorganism Mycobacterium phlei.

  2. Early Detection Saves Lives

    Broader source: Energy.gov [DOE]

    Former Department of Energy (DOE) workers tell how medical screening helped them lead healthier and longer lives.

  3. Field application of a genetically engineered microorganism for polycyclic aromatic hydrocarbon bioremediation process monitoring and control

    SciTech Connect (OSTI)

    Sayler, G.S.; Cox, C.D.; Ripp, S.; Nivens, D.E.; Werner, C.; Ahn, Y.; Matrubutham, U.; Burlage, R.

    1998-11-01

    On October 30, 1996, the US Environmental Protection Agency (EPA) commenced the first test release of genetically engineered microorganisms (GEMs) for use in bioremediation. The specific objectives of the investigation were multifaceted and include (1) testing the hypothesis that a GEM can be successfully introduced and maintained in a bioremediation process, (2) testing the concept of using, at the field scale, reporter organisms for direct bioremediation process monitoring and control, and (3) acquiring data that can be used in risk assessment decision making and protocol development for future field release applications of GEMs. The genetically engineered strain under investigation is Pseudomonas fluorescens strain HK44 (King et al., 1990). The original P. fluorescens parent strain was isolated from polycyclic aromatic hydrocarbon (PAH) contaminated manufactured gas plant soil. Thus, this bacterium is able to biodegrade naphthalene (as well as other substituted naphthalenes and other PAHs) and is able to function as a living bioluminescent reporter for the presence of naphthalene contamination, its bioavailability, and the functional process of biodegradation. A unique component of this field investigation was the availability of an array of large subsurface soil lysimeters. This article describes the experience associated with the release of a genetically modified microorganism, the lysimeter facility and its associated instrumentation, as well as representative data collected during the first eighteen months of operation.

  4. Scientist Take First X-Ray Portraits of Living Cyanobacteria at the LCLS

    SciTech Connect (OSTI)

    2015-02-11

    Researchers from Uppsala University working at the Department of Energy's SLAC National Accelerator Laboratory have captured the first X-ray portraits of living bacteria, detecting signals from features as small as 4 nanometers, or 4 billionths of a meter.

  5. Liquid Fuel from Heat-Loving Microorganisms: H2-Dependent Conversion of CO2 to Liquid Electrofuels by Extremely Thermophilic Archaea

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: NC State is working with the University of Georgia to create Electrofuels from primitive organisms called extremophiles that evolved before photosynthetic organisms and live in extreme, hot water environments with temperatures ranging from 167-212 degrees Fahrenheit The team is genetically engineering these microorganisms so they can use hydrogen to turn carbon dioxide directly into alcohol-based fuels. High temperatures are required to distill the biofuels from the water where the organisms live, but the heat-tolerant organisms will continue to thrive even as the biofuels are being distilledmaking the fuel-production process more efficient. The microorganisms dont require light, so they can be grown anywhereinside a dark reactor or even in an underground facility.

  6. Living in Los Alamos

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Living in Los Alamos Living in Los Alamos The enchanting surroundings, extraordinary people, and rich history make Los Alamos so much more than just a place to do great work. Discover the many advantages of living and working in Los Alamos. AROUND LOS ALAMOS Communication KRSN Community Radio Los Alamos Daily Post Los Alamos Monitor Education Los Alamos Public Schools Pajarito Environmental Education Center University of New Mexico - Los Alamos The Arts Fuller Lodge Art Center Los Alamos Little

  7. Dynamic Metabolism Studies of Live Bacterial Films

    SciTech Connect (OSTI)

    Majors, Paul D.; Mclean, Jeffrey S.

    2008-11-01

    Bacterial film (biofilm) microbes exist within spatial (nutrient, electron-acceptor, pH, etc.) gradients of their own making. Correspondingly, biofilm bacteria are physiologically and functionally distinct from free-floating bacteria and from their own species at differing biofilm depths. This article describes our efforts to develop noninvasive nuclear magnetic resonance (NMR) technologies for biofilm-metabolism studies. This involves integrating NMR with controlled-cultivation methods to interrogate microbial physiology live and under known growth conditions. NMR is uniquely capable of providing depth-resolved metabolic and transport information in a non-invasive, non-sample-consuming fashion, providing information required for experimental reactive transport studies. We have studied mono-species biofilms relevant to environment remediation and human health. We describe these technologies, discuss their advantages and limitations, and give examples of their application.

  8. Biofuels from Bacteria, Electricity, and CO2: Biofuels from CO2 Using Ammonia or Iron-Oxidizing Bacteria in Reverse Microbial Fuel Cells

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: Electrofuels Project: Columbia University is using carbon dioxide (CO2) from ambient air, ammoniaan abundant and affordable chemical, and a bacteria called N. europaea to produce liquid fuel. The Columbia University team is feeding the ammonia and CO2 into an engineered tank where the bacteria live. The bacteria capture the energy from ammonia and then use that energy to convert CO2 into a liquid fuel. When the bacteria use up all the ammonia, renewable electricity can regenerate it and pump it back into the systemcreating a continuous fuel-creation cycle. In addition, Columbia University is also working with the bacteria A. ferrooxidans to capture and use energy from ferrous iron to produce liquid fuels from CO2.

  9. Expansion of the Genomic Encyclopedia of Bacteria and Archaea

    SciTech Connect (OSTI)

    Rinke, Christian; Sczyrba, Alex; Malfatti, Stephanie; Lee, Janye; Cheng, Jan-Fang; Stepanauskas, Ramunas; Eisen, Jonathan A.; Hallam, Steven; Inskeep, William P.; Hedlund, Brian P.; Sievert, Stefan M.; Liu, Wen-Tso; Tsiamis, George; Hugenholtz, Philip; Woyke, Tanja

    2011-03-20

    To date the vast majority of bacterial and archaeal genomes sequenced are of rather limited phylogenetic diversity as they were chosen based on their physiology and/ or medical importance. The Genomic Encyclopedia of Bacteria and Archaea (GEBA) project (Wu et al. 2009) is aimed to systematically filling the gaps of the tree of life with phylogenetically diverse reference genomes. However more than 99percent of microorganisms elude current culturing attempts, severely limiting the ability to recover complete or even partial genomes of these largely mysterious species. These limitations gave rise to the GEBA uncultured project. Here we propose to use single cell genomics to massively expand the Genomic Encyclopedia of Bacteria and Archaea by targeting 80 single cell representatives of uncultured candidate phyla which have no or very few cultured representatives. Generating these reference genomes of uncultured microbes will dramatically increase the discovery rate of novel protein families and biological functions, shed light on the numerous underrepresented phyla that likely play important roles in the environment, and will assist in improving the reconstruction of the evolutionary history of Bacteria and Archaea. Moreover, these data will improve our ability to interpret metagenomics sequence data from diverse environments, which will be of tremendous value for microbial ecology and evolutionary studies to come.

  10. Expansion of the Genomic Encyclopedia of Bacteria and Archaea

    SciTech Connect (OSTI)

    Rinke, Christian; Sczyrba, Alex; Malfatti, Stephanie; Lee, Janey; Cheng, Jan-Fang; Stepanauskas, Ramunas; Eisen, Jonathan A.; Hallam, Steven; Inskeep, William P.; Hedlund, Brian P.; Sievert, Stefan M.; Liu, Wen-Tso; Tsiamis, George; Hugenholtz, Philip; Woyke, Tanja

    2011-06-02

    To date the vast majority of bacterial and archaeal genomes sequenced are of rather limited phylogenetic diversity as they were chosen based on their physiology and/ or medical importance. The Genomic Encyclopedia of Bacteria and Archaea (GEBA) project (Wu et al. 2009) is aimed at systematically filling the gaps of the tree of life with phylogenetically diverse reference genomes. However more than 99 percent of microorganisms elude current culturing attempts, severely limiting the ability to recover complete or even partial genomes of these largely mysterious species. These limitations gave rise to the GEBA uncultured project. Here we propose to use single cell genomics to massively expand the Genomic Encyclopedia of Bacteria and Archaea by targeting 80 single cell representatives of uncultured candidate phyla which have no or very few cultured representatives. Generating these reference genomes of uncultured microbes will dramatically increase the discovery rate of novel protein families and biological functions, shed light on the numerous underrepresented phyla that likely play important roles in the environment, and will assist in improving the reconstruction of the evolutionary history of Bacteria and Archaea. Moreover, these data will improve our ability to interpret metagenomics sequence data from diverse environments, which will be of tremendous value for microbial ecology and evolutionary studies to come.

  11. Novel microorganism for selective separation of coal from ash and pyrite; First quarterly technical progress report, September 1, 1993--November 30, 1993

    SciTech Connect (OSTI)

    Misra, M.; Smith, R.W.; Raichur, A.M.

    1993-12-31

    This report summarizes the progress made during the first quarter of the research project entitled ``A Novel Microorganism for Selective Separation of Coal from Ash and Pyrite,`` DOE Grant No. DE-FG22-93PC93215. The objective of this project is to study the effectiveness of a novel hydrophobic microorganism, Mycobacterium phlei (M. phlei), for the selective flocculation of coal from pyrite and ash-forming minerals. During the reporting period, three different coal samples: Illinois No. 6 coal, Kentucky No. 9 coal and Pittsburgh No. 8 coal, were collected to be used in the investigation. The microorganism, M. phlei, was obtained as freeze-dried cultures and the growth characteristics of the bacteria were studied. Scanning electron microphotographs revealed that M. phlei cells are coccal in shape and are approximately 1 {mu}m in diameter. Electrokinetic measurements showed that the Illinois No. 6 and Pittsburgh No. 8 coal samples had an isoelectric point (IEP) around pH 6 whereas M. phlei had an IEP around pH 1.5. Electrokinetic measurements of the ruptured microorganisms exhibited an increase in IEP. The increase in IEP of the ruputured cells was due to the release of fatty acids and polar groups from the cell membrane.

  12. Northern New Mexico Living

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Northern New Mexico Living Northern New Mexico Living The north end of the Land of Enchantment offers deserts, forests, and mountains, vibrant cities, and a rich culture and history Contact Us Email Living in Los Alamos Los Alamos, New Mexico is a community with a population of about 18,000. It sits on the Pajarito Plateau in Northern New Mexico between the city of White Rock and the Valles Caldera National Preserve. Lab employees who live in Los Alamos enjoy a 10-15 minute commute to work,

  13. NREL Explains the Higher Cellulolytic Activity of a Vital Microorganism |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bioenergy | NREL NREL Explains the Higher Cellulolytic Activity of a Vital Microorganism Wide range of cellulase modalities in C. thermocellum makes it one of the most efficient biomass degraders February 5, 2016 Researchers at the Energy Department's National Renewable Energy Laboratory (NREL) and the BioEnergy Science Center (BESC) say better understanding of a bacterium could lead to cheaper production of cellulosic ethanol and other advanced biofuels. Their discovery was made during an

  14. Aerobic microorganism for the degradation of chlorinated aliphatic hydrocarbons

    DOE Patents [OSTI]

    Fliermans, Carl B.

    1989-01-01

    A chlorinated aliphatic hydrocarbon-degrading microorganism, having American Type Culture Collection accession numbers ATCC 53570 and 53571, in a biologically pure culture aseptically collected from a deep subsurface habitat and enhanced, mineralizes trichloroethylene and tetrachloroethylene to HCl, H.sub.2 O and Co.sub.2 under aerobic conditions stimulated by methane, acetate, methanol, tryptone-yeast extract, propane and propane-methane.

  15. Cofermentation with Cooperative Microorganisms for More Efficient Biomass

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Conversion - Energy Innovation Portal Startup America Startup America Biomass and Biofuels Biomass and Biofuels Find More Like This Return to Search Cofermentation with Cooperative Microorganisms for More Efficient Biomass Conversion Oak Ridge National Laboratory Contact ORNL About This Technology Technology Marketing SummaryIt is well known that biomass has primarily two sources of fermentable carbohydrates, cellulose and hemicelluloses. Research has been underway for decades aimed at both

  16. Use of Stable Isotopes in Forensic Analysis of Microorganisms

    SciTech Connect (OSTI)

    Kreuzer-Martin, Helen W.; Hegg, Eric L.

    2012-01-18

    The use of isotopic signatures for forensic analysis of biological materials is well-established, and the same general principles that apply to interpretation of stable isotope content of C, N, O, and H apply to the analysis of microorganisms. Heterotrophic microorganisms derive their isotopic content from their growth substrates, which are largely plant and animal products, and the water in their culture medium. Thus the isotope signatures of microbes are tied to their growth environment. The C, N, O, and H isotope ratios of spores have been demonstrated to constitute highly discriminating signatures for sample matching. They can rule out specific samples of media and/or water as possible production media, and can predict isotope ratio ranges of the culture media and water used to produce a given sample. These applications have been developed and tested through analyses of approximately 250 samples of Bacillus subtilis spores and over 500 samples of culture media, providing a strong statistical basis for data interpretation. A Bayesian statistical framework for integrating stable isotope data with other types of signatures derived from microorganisms has been able to characterize the culture medium used to produce spores of various Bacillus species, leveraging isotopic differences in different medium types and demonstrating the power of data integration for forensic investigations.

  17. Potential of Diazorphic, Endophytic Bacteria Associated with...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Potential of Diazorphic, Endophytic Bacteria Associated with Sugarcane for Energycane Production Potential of Diazorphic, Endophytic Bacteria Associated with Sugarcane for Energycane ...

  18. Functionalized Polyacrylamide Monolith for Rapid Bacteria Pathogen...

    Office of Scientific and Technical Information (OSTI)

    for Rapid Bacteria Pathogen Detection in Human Blood. Citation Details In-Document Search Title: Functionalized Polyacrylamide Monolith for Rapid Bacteria Pathogen ...

  19. Computational modeling of drug-resistant bacteria. Final report

    SciTech Connect (OSTI)

    MacDougall, Preston

    2015-03-12

    Initial proposal summary: The evolution of antibiotic-resistant mutants among bacteria (superbugs) is a persistent and growing threat to public health. In many ways, we are engaged in a war with these microorganisms, where the corresponding arms race involves chemical weapons and biological targets. Just as advances in microelectronics, imaging technology and feature recognition software have turned conventional munitions into smart bombs, the long-term objectives of this proposal are to develop highly effective antibiotics using next-generation biomolecular modeling capabilities in tandem with novel subatomic feature detection software. Using model compounds and targets, our design methodology will be validated with correspondingly ultra-high resolution structure-determination methods at premier DOE facilities (single-crystal X-ray diffraction at Argonne National Laboratory, and neutron diffraction at Oak Ridge National Laboratory). The objectives and accomplishments are summarized.

  20. Lineman (Live Line)

    Broader source: Energy.gov [DOE]

    There are several Lineman (Liveline) positions located in Spokane, Washington. A successful candidate in this position will perform construction and maintenance work for live-line on wood and steel...

  1. Living SafeLy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    as safe as the electrical wiring in our homes - or just as danger- ous. The key is learning to act safely around them. This booklet is a basic safety guide for those who live...

  2. Living a Sustainable Future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Department of Energy Living Comfortably: A Consumer's Guide to Home Energy Upgrades Living Comfortably: A Consumer's Guide to Home Energy Upgrades March 7, 2013 - 3:15pm Addthis A weatherization worker drills holes to blow cellulose insulation in the interior walls of this home. | Photo courtesy of Dennis Schroeder, NREL A weatherization worker drills holes to blow cellulose insulation in the interior walls of this home. | Photo courtesy of Dennis Schroeder, NREL Dr. Richard Knaub Project

  3. Effects of remediation amendments on vadose zone microorganisms

    SciTech Connect (OSTI)

    Miller, Hannah M.; Tilton, Fred A.

    2012-08-10

    Surfactant-based foam delivery technology has been studied to remediate Hanford 200 area deep vadose zone sediment. However, the surfactants and remediation amendments have an unknown effect on indigenous subsurface microorganisms. Microbial populations are important factors to consider in remediation efforts due to their potential to alter soil geochemistry. This project focuses on measuring microbial metabolic responses to remediation amendments in batch and column studies using Deep Vadose Zone Sediments. Initial studies of the microbes from Hanford 200 area deep vadose zone sediment showed surfactants sodium dodecyl sulfate (SDS) and cocamidopropyl betaine (CAPB) and remediation amendment calcium polysulfide (CPS) had no affect on microbial growth using BiologTM Ecoplates. To move towards a more realistic field analog, soil columns were packed with Hanford 200 Area sediment. Once microbial growth in the column was verified by observing growth of the effluent solution on tryptic soy agar plates, remedial surfactants were injected into the columns, and the resulting metabolic diversity was measured. Results suggest surfactant sodium dodecyl sulfate (SDS) stimulates microbial growth. The soil columns were also visualized using X-ray microtomography to inspect soil packing and possibly probe for evidence of biofilms. Overall, BiologTM Ecoplates provide a rapid assay to predict effects of remediation amendments on Hanford 200 area deep vadose zone microorganisms.

  4. Organic acid-tolerant microorganisms and uses thereof for producing organic acids

    SciTech Connect (OSTI)

    Pfleger, Brian Frederick; Begemann, Matthew Brett

    2014-05-06

    Organic acid-tolerant microorganisms and methods of using same. The organic acid-tolerant microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid (3HP), acrylic acid, and propionic acid. Further modifications to the microorganisms such as increasing expression of malonyl-CoA reductase and/or acetyl-CoA carboxylase provide or increase the ability of the microorganisms to produce 3HP. Methods of generating an organic acid with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers include replacing acsA or homologs thereof in cells with genes of interest and selecting for the cells comprising the genes of interest with amounts of organic acids effective to inhibit growth of cells harboring acsA or the homologs.

  5. Methods for identifying an essential gene in a prokaryotic microorganism

    DOE Patents [OSTI]

    Shizuya, Hiroaki

    2006-01-31

    Methods are provided for the rapid identification of essential or conditionally essential DNA segments in any species of haploid cell (one copy chromosome per cell) that is capable of being transformed by artificial means and is capable of undergoing DNA recombination. This system offers an enhanced means of identifying essential function genes in diploid pathogens, such as gram-negative and gram-positive bacteria.

  6. Sensory Transduction in Microorganisms 2008 Gordon Research Conference (January 2008)

    SciTech Connect (OSTI)

    Ann M. Stock

    2009-04-08

    Research into the mechanisms involved in the sensing and responses of microorganisms to changes in their environments is currently very active in a large number of laboratories worldwide. An increasingly wide range of prokaryotic and eukaryotic species are being studied with regard to their sensing of diverse chemical and physical stimuli, including nutrients, toxins, intercellular signaling molecules, redox indicators, light, pressure, magnetic fields, and surface contact, leading to adaptive responses affecting motile behavior, gene expression and/or development. The ease of manipulation of microorganisms has facilitated application of a broad range of techniques that have provided comprehensive descriptions of cellular behavior and its underlying molecular mechanisms. Systems and their molecular components have been probed at levels ranging from the whole organism down to atomic resolution using behavioral analyses; electrophysiology; genetics; molecular biology; biochemical and biophysical characterization; structural biology; single molecule, fluorescence and cryo-electron microscopy; computational modeling; bioinformatics and genomic analyses. Several model systems such as bacterial chemotaxis and motility, fruiting body formation in Myxococcus xanthus, and motility and development in Dictyostelium discoideum have traditionally been a focus of this meeting. By providing a basis for assessment of similarities and differences in mechanisms, understanding of these pathways has advanced the study of many other microbial sensing systems. This conference aims to bring together researchers investigating different prokaryotic and eukaryotic microbial systems using diverse approaches to compare data, share methodologies and ideas, and seek to understand the fundamental principles underlying sensory responses. Topic areas include: (1) Receptor Sensing and Signaling; (2) Intracellular Signaling (two-component, c-di-GMP, c-AMP, etc.); (3) Intracellular Localization and

  7. Re-engineering bacteria for ethanol production

    DOE Patents [OSTI]

    Yomano, Lorraine P; York, Sean W; Zhou, Shengde; Shanmugam, Keelnatham; Ingram, Lonnie O

    2014-05-06

    The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.

  8. Utilizing Bacteria for Sustainable Manufacturing of Low-Cost...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Utilizing Bacteria for Sustainable Manufacturing of Low-Cost Nanoparticles Utilizing Bacteria for Sustainable Manufacturing of Low-Cost Nanoparticles Utilizing Bacteria for ...

  9. Engineering Biofuels from Photosynthetic Bacteria | Argonne National

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laboratory Engineering Biofuels from Photosynthetic Bacteria Technology available for licensing: Using photosynthetic bacteria to produce biofuels. 30-70% of the fuel's waste can be used to create other fuel sources Combines both engineered and natural photosynthetic mechanisms to generate the fuel PDF icon biofuels_from_bacteria

  10. Apparatus and method for transforming living cells

    DOE Patents [OSTI]

    Okandan, Murat; Galambos, Paul C.

    2003-11-11

    An apparatus and method are disclosed for in vitro transformation of living cells. The apparatus, which is formed as a microelectromechanical device by surface micromachining, can be used to temporarily disrupt the cell walls or membrane of host cells one at a time so that a particular substance (e.g. a molecular tag, nucleic acid, bacteria, virus etc.) can be introduced into the cell. Disruption of the integrity of the host cells (i.e. poration) can be performed mechanically or electrically, or by both while the host cells are contained within a flow channel. Mechanical poration is possible using a moveable member which has a pointed or serrated edge and which is driven by an electrostatic actuator to abrade, impact or penetrate the host cell. Electroporation is produced by generating a relatively high electric field across the host cell when the host cell is located in the flow channel between a pair of electrodes having a voltage applied therebetween.

  11. Fuel Cells Go Live

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    green h y d r o g e n f u e l i n g POWer Fuel Cells Go live A closer look at the requirements to create a hydrogen-based warehouse M anagers of distribution centers are always on the lookout for new ways to gain competitive advantage through increased operational efficiency, productivity and worker safety. Around North America, some are finding success by integrating commercially available hydrogen fuel cell systems into their lift truck fleets. For operations with large fleets of electric lift

  12. Living olefin polymerization processes

    DOE Patents [OSTI]

    Schrock, Richard R.; Bauman, Robert

    2006-11-14

    Processes for the living polymerization of olefin monomers with terminal carbon-carbon double bonds are disclosed. The processes employ initiators that include a metal atom and a ligand having two group 15 atoms and a group 16 atom or three group 15 atoms. The ligand is bonded to the metal atom through two anionic or covalent bonds and a dative bond. The initiators are particularly stable under reaction conditions in the absence of olefin monomer. The processes provide polymers having low polydispersities, especially block copolymers having low polydispersities. It is an additional advantage of these processes that, during block copolymer synthesis, a relatively small amount of homopolymer is formed.

  13. Living olefin polymerization processes

    DOE Patents [OSTI]

    Schrock, Richard R.; Baumann, Robert

    2003-08-26

    Processes for the living polymerization of olefin monomers with terminal carbon-carbon double bonds are disclosed. The processes employ initiators that include a metal atom and a ligand having two group 15 atoms and a group 16 atom or three group 15 atoms. The ligand is bonded to the metal atom through two anionic or covalent bonds and a dative bond. The initiators are particularly stable under reaction conditions in the absence of olefin monomer. The processes provide polymers having low polydispersities, especially block copolymers having low polydispersities. It is an additional advantage of these processes that, during block copolymer synthesis, a relatively small amount of homopolymer is formed.

  14. Living olefin polymerization processes

    DOE Patents [OSTI]

    Schrock, Richard R. (Winchester, MA); Baumann, Robert (Cambridge, MA)

    1999-01-01

    Processes for the living polymerization of olefin monomers with terminal carbon-carbon double bonds are disclosed. The processes employ initiators that include a metal atom and a ligand having two group 15 atoms and a group 16 atom or three group 15 atoms. The ligand is bonded to the metal atom through two anionic or covalent bonds and a dative bond. The initiators are particularly stable under reaction conditions in the absence of olefin monomer. The processes provide polymers having low polydispersities, especially block copolymers having low polydispersities. It is an additional advantage of these processes that, during block copolymer synthesis, a relatively small amount of homopolymer is formed.

  15. Living olefin polymerization processes

    DOE Patents [OSTI]

    Schrock, R.R.; Baumann, R.

    1999-03-30

    Processes for the living polymerization of olefin monomers with terminal carbon-carbon double bonds are disclosed. The processes employ initiators that include a metal atom and a ligand having two group 15 atoms and a group 16 atom or three group 15 atoms. The ligand is bonded to the metal atom through two anionic or covalent bonds and a dative bond. The initiators are particularly stable under reaction conditions in the absence of olefin monomer. The processes provide polymers having low polydispersities, especially block copolymers having low polydispersities. It is an additional advantage of these processes that, during block copolymer synthesis, a relatively small amount of homopolymer is formed.

  16. Engineering and Coordination of Regulatory Networks and Intracellular Complexes to Maximize Hydrogen Production by Phototrophic Microorganisms

    SciTech Connect (OSTI)

    James C. Liao

    2012-05-22

    This project is a collaboration with F. R. Tabita of Ohio State. Our major goal is to understand the factors and regulatory mechanisms that influence hydrogen production. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Our part of the project was to develop a modeling technique to investigate the metabolic network in connection to hydrogen production and regulation. Organisms must balance the pathways that generate and consume reducing power in order to maintain redox homeostasis to achieve growth. Maintaining this homeostasis in the nonsulfur purple photosynthetic bacteria is a complex feat with many avenues that can lead to balance, as these organisms possess versatile metabolic capabilities including anoxygenic photosynthesis, aerobic or anaerobic respiration, and fermentation. Growth is achieved by using H{sub 2} as an electron donor and CO{sub 2} as a carbon source during photoautotrophic and chemoautotrophic growth, where CO{sub 2} is fixed via the Calvin-Benson-Bassham (CBB) cycle. Photoheterotrophic growth can also occur when alternative organic carbon compounds are utilized as both the carbon source and electron donor. Regardless of the growth mode, excess reducing equivalents generated as a result of oxidative processes, must be transferred to terminal electron acceptors, thus insuring that redox homeostasis is maintained in the cell. Possible terminal acceptors include O{sub 2}, CO{sub 2}, organic carbon, or various oxyanions. Cells possess regulatory mechanisms to balance the activity of the pathways which supply energy, such as photosynthesis, and those that consume energy, such as CO{sub 2} assimilation or N{sub 2} fixation. The major route for CO{sub 2} assimilation is the CBB

  17. Correlative Electron and Fluorescence Microscopy of Magnetotactic Bacteria in Liquid: Toward In Vivo Imaging

    SciTech Connect (OSTI)

    Woehl, Taylor J.; Kashyap, Sanjay; Firlar, Emre; Perez-Gonzalez, Teresa; Faivre, Damien; Trubitsyn, Denis; Bazylinski, Dennis A.; Prozorov, Tanya

    2014-10-31

    Magnetotactic bacteria biomineralize ordered chains of uniform, membrane-bound magnetite or greigite nanocrystals that exhibit nearly perfect crystal structures and species-specific morphologies. Transmission electron microscopy (TEM) is a critical technique for providing information regarding the organization of cellular and magnetite structures in these microorganisms. However, conventional TEM can only be used to image air-dried or vitrified bacteria removed from their natural environment. Here we present a correlative scanning TEM (STEM) and fluorescence microscopy technique for imaging viable cells of Magnetospirillum magneticum strain AMB-1 in liquid using an in situ fluid cell TEM holder. Fluorescently labeled cells were immobilized on microchip window surfaces and visualized in a fluid cell with STEM, followed by correlative fluorescence imaging to verify their membrane integrity. Notably, the post-STEM fluorescence imaging indicated that the bacterial cell wall membrane did not sustain radiation damage during STEM imaging at low electron dose conditions. We investigated the effects of radiation damage and sample preparation on the bacteria viability and found that approximately 50% of the bacterial membranes remained intact after an hour in the fluid cell, decreasing to ~30% after two hours. These results represent a first step toward in vivo studies of magnetite biomineralization in magnetotactic bacteria.

  18. Correlative Electron and Fluorescence Microscopy of Magnetotactic Bacteria in Liquid: Toward In Vivo Imaging

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Woehl, Taylor J.; Kashyap, Sanjay; Firlar, Emre; Perez-Gonzalez, Teresa; Faivre, Damien; Trubitsyn, Denis; Bazylinski, Dennis A.; Prozorov, Tanya

    2014-10-31

    Magnetotactic bacteria biomineralize ordered chains of uniform, membrane-bound magnetite or greigite nanocrystals that exhibit nearly perfect crystal structures and species-specific morphologies. Transmission electron microscopy (TEM) is a critical technique for providing information regarding the organization of cellular and magnetite structures in these microorganisms. However, conventional TEM can only be used to image air-dried or vitrified bacteria removed from their natural environment. Here we present a correlative scanning TEM (STEM) and fluorescence microscopy technique for imaging viable cells of Magnetospirillum magneticum strain AMB-1 in liquid using an in situ fluid cell TEM holder. Fluorescently labeled cells were immobilized on microchip windowmore » surfaces and visualized in a fluid cell with STEM, followed by correlative fluorescence imaging to verify their membrane integrity. Notably, the post-STEM fluorescence imaging indicated that the bacterial cell wall membrane did not sustain radiation damage during STEM imaging at low electron dose conditions. We investigated the effects of radiation damage and sample preparation on the bacteria viability and found that approximately 50% of the bacterial membranes remained intact after an hour in the fluid cell, decreasing to ~30% after two hours. These results represent a first step toward in vivo studies of magnetite biomineralization in magnetotactic bacteria.« less

  19. Prediction of substrate removal rates of attached microorganisms and of relative contributions of attached and suspended communities at field sites

    SciTech Connect (OSTI)

    Lewis, D.L.; Gattie, D.K.

    1988-02-01

    A mathematical model composed of a direct proportionality relationship between bulk water velocities and field-determined second-order microbial transformation rate coefficients, and the relative rate coefficient of a benchmark chemical, was developed for estimating the substrate removal rates of rapidly degraded chemicals by attached microorganisms in shallow (less than 1 m deep) aquatic ecosystems. Data from 31 field experiments involving the addition of 2,4-dichlorophenoxyacetic acid methyl ester (2,4-DME) in nine field areas were used to determine a field-derived second-order rate coefficient for microbial transformation of the ester. By using 2,4-DME as a benchmark chemical, the model was used to predict microbial transformation rates of the butoxyethyl ester of 2,4-dichlorophenoxyacetic acid (2,4-DBE) at five other field sites. The predicted half-lives of 2,4-DBE varied 1500-fold and were within about a 3-fold range or less of the measured half-lives.

  20. Final Technical Report: Viral Infection of Subsurface Microorganisms and Metal/Radionuclide Transport

    SciTech Connect (OSTI)

    Weber, Karrie A.; Bender, Kelly S.; Li, Yusong

    2013-09-28

    Microbially mediated metabolisms have been identified as a significant factor either directly or indirectly impacting the fate and transport of heavy metal/radionuclide contaminants. To date microorganisms have been isolated from contaminated environments. Examination of annotated finished genome sequences of many of these subsurface isolates from DOE sites, revealed evidence of prior viral infection. To date the role that viruses play influencing microbial mortality and the resulting community structure which directly influences biogeochemical cycling in soils and sedimentary environments remains poorly understood. The objective of this exploratory study was to investigate the role of viral infection of subsurface bacteria and the formation of contaminant-bearing viral particles. This objective was approached by examining the following working hypotheses: (i) subsurface microorganisms are susceptible to viral infections by the indigenous subsurface viral community, and (ii) viral surfaces will adsorb heavy metals and radionuclides. Our results have addressed basic research needed to accomplish the BER Long Term Measure to provide sufficient scientific understanding such that DOE sites would be able to incorporate coupled physical, chemical and biological processes into decision making for environmental remediation or natural attenuation and long-term stewardship by establishing viral-microbial relationships on the subsequent fate and transport of heavy metals and radionuclides. Here we demonstrated that viruses play a significant role in microbial mortality and community structure in terrestrial subsurface sedimentary systems. The production of viral-like particles within subsurface sediments in response to biostimulation with dissolved organic carbon and a terminal electron acceptor resulted in the production of viral-like particles. Organic carbon alone did not result in significant viral production and required the addition of a terminal electron acceptor

  1. Transformation of gram positive bacteria by sonoporation

    DOE Patents [OSTI]

    Yang, Yunfeng; Li, Yongchao

    2014-03-11

    The present invention provides a sonoporation-based method that can be universally applied for delivery of compounds into Gram positive bacteria. Gram positive bacteria which can be transformed by sonoporation include, for example, Bacillus, Streptococcus, Acetobacterium, and Clostridium. Compounds which can be delivered into Gram positive bacteria via sonoporation include nucleic acids (DNA or RNA), proteins, lipids, carbohydrates, viruses, small organic and inorganic molecules, and nano-particles.

  2. Spectroscopic diagnostics for bacteria in biologic sample

    DOE Patents [OSTI]

    El-Sayed, Mostafa A.; El-Sayed, Ivan H.

    2002-01-01

    A method to analyze and diagnose specific bacteria in a biologic sample using spectroscopy is disclosed. The method includes obtaining the spectra of a biologic sample of a non-infected patient for use as a reference, subtracting the reference from the spectra of an infected sample, and comparing the fingerprint regions of the resulting differential spectrum with reference spectra of bacteria in saline. Using this diagnostic technique, specific bacteria can be identified sooner and without culturing, bacteria-specific antibiotics can be prescribed sooner, resulting in decreased likelihood of antibiotic resistance and an overall reduction of medical costs.

  3. Nucleic acid molecules conferring enhanced ethanol tolerance and microorganisms having enhanced tolerance to ethanol

    DOE Patents [OSTI]

    Brown, Steven; Guss, Adam; Yang, Shihui; Karpinets, Tatiana; Lynd, Lee; Shao, Xiongjun

    2014-01-14

    The present invention provides isolated nucleic acid molecules which encode a mutant acetaldehyde-CoA/alcohol dehydrogenase or mutant alcohol dehydrogenase and confer enhanced tolerance to ethanol. The invention also provides related expression vectors, genetically engineered microorganisms having enhanced tolerance to ethanol, as well as methods of making and using such genetically modified microorganisms for production of biofuels based on fermentation of biomass materials.

  4. Synthetic Design Microorganisms for Lignin Fuels and Chemicals Presentation for BETO 2015 Project Peer Review

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Synthetic Design Microorganisms for Lignin Fuels and Chemicals 3/26/2015 Synthetic Biology Joshua S. Yuan Associate Professor and Director Texas A&M University This presentation does contain proprietary information 1 Project Goal: Design of Microorganisms for Lignin Fuel * The proposed research aims to address one of the most challenging issues in biofuel production: the utilization of lignin for fungible fuels. * Project Outcome: A viable biological platform for conversion of lignin into

  5. Hydrogen metabolism of photosynthetic bacteria and algae

    SciTech Connect (OSTI)

    Kumazawa, S.; Mitsui, A.

    1982-01-01

    The metabolism, metabolic pathways and biochemistry of hydrogen in photosynthetic bacteria and algae are reviewed. Detailed information on the occurrence and measurement of hydrogenase activity is presented. Hydrogen production rates for different species of algae and bacteria are presented. 173 references, 1 figure, 7 tables.

  6. Method of dispersing a hydrocarbon using bacteria

    DOE Patents [OSTI]

    Tyndall, R.L.

    1996-09-24

    A new protozoan derived microbial consortia and method for their isolation are provided. The isolated consortia and bacteria are useful for treating wastes such as trichloroethylene and trinitrotoluene. The isolated consortia, bacteria, and dispersants are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  7. Method of dispersing a hydrocarbon using bacteria

    DOE Patents [OSTI]

    Tyndall, Richard L.

    1996-01-01

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  8. Chemotactic selection of pollutant degrading soil bacteria

    DOE Patents [OSTI]

    Hazen, T.C.

    1991-03-04

    A method is described for identifying soil microbial strains which may be bacterial degraders of pollutants. This method includes: Placing a concentration of a pollutant in a substantially closed container; placing the container in a sample of soil for a period of time ranging from one minute to several hours; retrieving the container and collecting its contents; microscopically determining the identity of the bacteria present. Different concentrations of the pollutant can be used to determine which bacteria respond to each concentration. The method can be used for characterizing a polluted site or for looking for naturally occurring biological degraders of the pollutant. Then bacteria identified as degraders of the pollutant and as chemotactically attracted to the pollutant are used to innoculate contaminated soil. To enhance the effect of the bacteria on the pollutant, nutrients are cyclicly provided to the bacteria then withheld to alternately build up the size of the bacterial colony or community and then allow it to degrade the pollutant.

  9. Metabolic Engineering and Modeling of Metabolic Pathways to Improve Hydrogen Production by Photosynthetic Bacteria

    SciTech Connect (OSTI)

    Jiao, Y.; Navid, A.

    2014-12-19

    Rising energy demands and the imperative to reduce carbon dioxide (CO2) emissions are driving research on biofuels development. Hydrogen gas (H2) is one of the most promising biofuels and is seen as a future energy carrier by virtue of the fact that 1) it is renewable, 2) does not evolve the “greenhouse gas” CO2 in combustion, 3) liberates large amounts of energy per unit weight in combustion (having about 3 times the energy content of gasoline), and 4) is easily converted to electricity by fuel cells. Among the various bioenergy strategies, environmental groups and others say that the concept of the direct manufacture of alternative fuels, such as H2, by photosynthetic organisms is the only biofuel alternative without significant negative criticism [1]. Biological H2 production by photosynthetic microorganisms requires the use of a simple solar reactor such as a transparent closed box, with low energy requirements, and is considered as an attractive system to develop as a biocatalyst for H2 production [2]. Various purple bacteria including Rhodopseudomonas palustris, can utilize organic substrates as electron donors to produce H2 at the expense of solar energy. Because of the elimination of energy cost used for H2O oxidation and the prevention of the production of O2 that inhibits the H2-producing enzymes, the efficiency of light energy conversion to H2 by anoxygenic photosynthetic bacteria is in principle much higher than that by green algae or cyanobacteria, and is regarded as one of the most promising cultures for biological H2 production [3]. Here implemented a simple and relatively straightforward strategy for hydrogen production by photosynthetic microorganisms using sunlight, sulfur- or iron-based inorganic substrates, and CO2 as the feedstock. Carefully selected microorganisms with bioengineered beneficial

  10. Advanced Biofuels: How Scientists are Engineering Bacteria to...

    Office of Environmental Management (EM)

    Advanced Biofuels: How Scientists are Engineering Bacteria to Help Drive America Advanced Biofuels: How Scientists are Engineering Bacteria to Help Drive America December 6, 2011 - ...

  11. Copy of Synthetic Biology of Novel Thermophilic Bacteria for...

    Office of Scientific and Technical Information (OSTI)

    Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of ... Title: Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of ...

  12. Discovery of functional toxin/antitoxin systems in bacteria by...

    Office of Scientific and Technical Information (OSTI)

    ...antitoxin systems in bacteria by shotgun cloning Citation Details In-Document Search Title: Discovery of functional toxinantitoxin systems in bacteria by shotgun cloning ...

  13. Bacteria increase arid-land soil surface temperature through...

    Office of Scientific and Technical Information (OSTI)

    Bacteria increase arid-land soil surface temperature through the production of sunscreens Prev Next Title: Bacteria increase arid-land soil surface temperature through the ...

  14. Xylan utilization in human gut commensal bacteria is orchestrated...

    Office of Scientific and Technical Information (OSTI)

    Xylan utilization in human gut commensal bacteria is orchestrated by unique modular ... Title: Xylan utilization in human gut commensal bacteria is orchestrated by unique modular ...

  15. Estimating Bacteria Emissions from Inversion of Atmospheric Transport...

    Office of Scientific and Technical Information (OSTI)

    Bacteria Emissions from Inversion of Atmospheric Transport: Sensitivity to Modelled Particle Characteristics Citation Details In-Document Search Title: Estimating Bacteria ...

  16. Methane and Methanotrophic Bacteria as a Biotechnological Platform...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Methane and Methanotrophic Bacteria as a Biotechnological Platform Methane and Methanotrophic Bacteria as a Biotechnological Platform Breakout Session 2-B: NewEmerging Pathways ...

  17. How Bacteria Make Magnets | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    How Bacteria Make Magnets For a number of animals, including birds, fish and mammals, there is evidence that magnets are used for orientation. However, little is known about how...

  18. Methods for dispersing hydrocarbons using autoclaved bacteria

    DOE Patents [OSTI]

    Tyndall, Richard L.

    1996-01-01

    A method of dispersing a hydrocarbon includes the steps: providing a bacterium selected from the following group: ATCC 85527, ATCC 75529, and ATCC 55638, a mutant of any one of these bacteria possessing all the identifying characteristics of any one of these bacteria, and mixtures thereof; autoclaving the bacterium to derive a dispersant solution therefrom; and contacting the dispersant solution with a hydrocarbon to disperse the hydrocarbon. Moreover, a method for preparing a dispersant solution includes the following steps: providing a bacterium selected from the following group: ATCC 75527, ATCC 75529, and ATCC 55638, a mutant of any one of these bacteria possessing all the identifying characteristics of any one of these bacteria, and mixtures thereof; and autoclaving the bacterium to derive a dispersant solution therefrom.

  19. Methods for dispersing hydrocarbons using autoclaved bacteria

    DOE Patents [OSTI]

    Tyndall, R.L.

    1996-11-26

    A method of dispersing a hydrocarbon includes the following steps: providing a bacterium selected from the following group: ATCC 85527, ATCC 75529, and ATCC 55638, a mutant of any one of these bacteria possessing all the identifying characteristics of any one of these bacteria, and mixtures; autoclaving the bacterium to derive a dispersant solution; and contacting the dispersant solution with a hydrocarbon to disperse the hydrocarbon. Moreover, a method for preparing a dispersant solution includes the following steps: providing a bacterium selected from the following group: ATCC 75527, ATCC 75529, and ATCC 55638, a mutant of any one of these bacteria possessing all the identifying characteristics of any one of these bacteria, and mixtures; and autoclaving the bacterium to derive a dispersant solution.

  20. Comparative genomics of the lactic acid bacteria

    SciTech Connect (OSTI)

    Makarova, K.; Slesarev, A.; Wolf, Y.; Sorokin, A.; Mirkin, B.; Koonin, E.; Pavlov, A.; Pavlova, N.; Karamychev, V.; Polouchine, N.; Shakhova, V.; Grigoriev, I.; Lou, Y.; Rokhsar, D.; Lucas, S.; Huang, K.; Goodstein, D. M.; Hawkins, T.; Plengvidhya, V.; Welker, D.; Hughes, J.; Goh, Y.; Benson, A.; Baldwin, K.; Lee, J. -H.; Diaz-Muniz, I.; Dosti, B.; Smeianov, V; Wechter, W.; Barabote, R.; Lorca, G.; Altermann, E.; Barrangou, R.; Ganesan, B.; Xie, Y.; Rawsthorne, H.; Tamir, D.; Parker, C.; Breidt, F.; Broadbent, J.; Hutkins, R.; O'Sullivan, D.; Steele, J.; Unlu, G.; Saier, M.; Klaenhammer, T.; Richardson, P.; Kozyavkin, S.; Weimer, B.; Mills, D.

    2006-06-01

    Lactic acid-producing bacteria are associated with various plant and animal niches and play a key role in the production of fermented foods and beverages. We report nine genome sequences representing the phylogenetic and functional diversity of these bacteria. The small genomes of lactic acid bacteria encode a broad repertoire of transporters for efficient carbon and nitrogen acquisition from the nutritionally rich environments they inhabit and reflect a limited range of biosynthetic capabilities that indicate both prototrophic and auxotrophic strains. Phylogenetic analyses, comparison of gene content across the group, and reconstruction of ancestral gene sets indicate a combination of extensive gene loss and key gene acquisitions via horizontal gene transfer during the coevolution of lactic acid bacteria with their habitats.

  1. Fuel from Bacteria: Bioconversion of Carbon Dioxide to Biofuels by Facultatively Autotrophic Hydrogen Bacteria

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: Ohio State is genetically modifying bacteria to efficiently convert carbon dioxide directly into butanol, an alcohol that can be used directly as a fuel blend or converted to a hydrocarbon, which closely resembles a gasoline. Bacteria are typically capable of producing a certain amount of butanol before it becomes too toxic for the bacteria to survive. Ohio State is engineering a new strain of the bacteria that could produce up to 50% more butanol before it becomes too toxic for the bacteria to survive. Finding a way to produce more butanol more efficiently would significantly cut down on biofuel production costs and help make butanol cost competitive with gasoline. Ohio State is also engineering large tanks, or bioreactors, to grow the biofuel-producing bacteria in, and they are developing ways to efficiently recover biofuel from the tanks.

  2. Deep subsurface life from North Pond: Enrichment, isolation, characterization and genomes of heterotrophic bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Russell, Joseph A.; Leon-Zayas, Rosa; Wrighton, Kelly; Biddle, Jennifer F.

    2016-05-10

    Studies of subsurface microorganisms have yielded few environmentally relevant isolates for laboratory studies. In order to address this lack of cultivated microorganisms, we initiated several enrichments on sediment and underlying basalt samples from North Pond, a sediment basin ringed by basalt outcrops underlying an oligotrophic watercolumn west of the Mid-Atlantic Ridge at 22° N. In contrast to anoxic enrichments, growth was observed in aerobic, heterotrophic enrichments from sediment of IODP Hole U1382B at 4 and 68 m below seafloor (mbsf). These sediment depths, respectively, correspond to the fringes of oxygen penetration from overlying seawater in the top of the sedimentmore » column and upward migration of oxygen from oxic seawater from the basalt aquifer below the sediment. Here we report the enrichment, isolation, initial characterization and genomes of three isolated aerobic heterotrophs from North Pond sediments; an Arthrobacter species from 4 mbsf, and Paracoccus and Pseudomonas species from 68 mbsf. These cultivated bacteria are represented in the amplicon 16S rRNA gene libraries created from whole sediments, albeit at low (up to 2%) relative abundance. We provide genomic evidence from our isolates demonstrating that the Arthrobacter and Pseudomonas isolates have the potential to respire nitrate and oxygen, though dissimilatory nitrate reduction could not be confirmed in laboratory cultures. Furthermore, the cultures from this study represent members of abundant phyla, as determined by amplicon sequencing of environmental DNA extracts, and allow for further studies into geochemical factors impacting life in the deep subsurface.« less

  3. Phase Preference by Active, Acetate-Utilizing Bacteria at the Rifle, CO Integrated Field Research Challenge Site

    SciTech Connect (OSTI)

    Kerkhoff, Lee; Williams, Kenneth H.; Long, Philip E.; McGuinness, L.

    2011-02-15

    Uranium contaminated groundwaters are a legacy concern for the U.S. Department of Energy. Previous experiments at the Rifle, Colorado Integrated Field Challenge (IFC) site have demonstrated that field-scale addition of acetate to groundwater reduces the ambient soluable uranium concentration, sequestering the radionuclide as uraninite. However, questions remain regarding which microorganism(s) are consuming this acetate and if active groundwater microorganisms are different from active particle-associated bacteria. In this report, 13-C acetate was used to assess the active microbes that synthesize DNA on 3 size fractions [coarse sand, fines (8-approximately 150 micron), groundwater (0.2-8 micron)] over a 24 -day time frame. Results indicated a stronger signal from 13-C acetate associated with the fines fraction compared with smaller amounts of 13-C uptake on the sand fraction and groundwater samples during the SIP incubations. TRFLP analysis of this 13-C-labeled DNA, indicated 31+ 9 OTU's with 6 peaks dominating the active profiles (166, 187, 210, 212, and 277 bp peaks using MnlI). Cloning/sequencing of the amplification products indicated a Geobacter-like group (187, 210, 212 bp) primarily synthesized DNA from acetate in the groundwater phase, an alpha Proteobacterium (166 bp) primarily grew on the fines/sands, and an Acinetobacter sp. (277 bp) utilized much of the 13C acetate in both groundwater and particle-associated phases. These findings will help to delineate the acetate utilization patterns of bacteria during field-scale acetate addition and can lead to improved methods for stimulating distinct microbial populations in situ.

  4. Living Buildings Ltd | Open Energy Information

    Open Energy Info (EERE)

    Ltd Jump to: navigation, search Name: Living Buildings Ltd Place: United Kingdom Sector: Buildings Product: Living Buildings is an installer of small-scale distributed generation...

  5. Living Villages Holdings Ltd | Open Energy Information

    Open Energy Info (EERE)

    Holdings Ltd Jump to: navigation, search Name: Living Villages Holdings Ltd Place: England, United Kingdom Zip: Shropshire SY9 Product: Living Villages is a residential property...

  6. Solar Living Institute | Open Energy Information

    Open Energy Info (EERE)

    Living Institute Jump to: navigation, search Name: Solar Living Institute Address: 13771 S. Hwy. 101 Place: Hopland, California Zip: 95449 Region: Bay Area Website:...

  7. Nuclear Physics Technology Saves Lives | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Physics Technology Saves Lives Nuclear Physics Technology Saves Lives January 11, 2006 ... The basic technology is called nuclear functional imaging. Jefferson Lab's Detector and ...

  8. Chemotactic selection of pollutant degrading soil bacteria

    DOE Patents [OSTI]

    Hazen, Terry C.

    1994-01-01

    A method for identifying soil microbial strains which may be bacterial degraders of pollutants comprising the steps of placing a concentration of a pollutant in a substantially closed container, placing the container in a sample of soil for a period of time ranging from one minute to several hours, retrieving the container, collecting the contents of the container, and microscopically determining the identity of the bacteria present. Different concentrations of the pollutant can be used to determine which bacteria respond to each concentration. The method can be used for characterizing a polluted site or for looking for naturally occurring biological degraders of the pollutant. Then bacteria identified as degraders of the pollutant and as chemotactically attracted to the pollutant are used to inoculate contaminated soil. To enhance the effect of the bacteria on the pollutant, nutrients are cyclicly provided to the bacteria then withheld to alternately build up the size of the bacterial colony or community and then allow it to degrade the pollutant.

  9. The Brief Lives of Neutrons

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Brief Lives of Neutrons 1663 Los Alamos science and technology magazine Latest Issue:July 2016 past issues All Issues » submit The Brief Lives of Neutrons A unique experiment to pin down the neutron's surprisingly elusive half-life July 21, 2016 Most neutrons inside atoms are stable. But get one on its own, and it will disintegrate in about ten minutes. Most neutrons inside atoms are stable. But get one on its own, and it will disintegrate in about ten minutes. Beam and bottle experiments

  10. Functional Encyclopedia of Bacteria and Archaea

    SciTech Connect (OSTI)

    Blow, M. J.; Deutschbauer, A. M.; Hoover, C. A.; Lamson, J.; Lamson, J.; Price, M. N.; Waters, J.; Wetmore, K. M.; Bristow, J.; Arkin, A. P.

    2013-03-20

    Bacteria and Archaea exhibit a huge diversity of metabolic capabilities with fundamental importance in the environment, and potential applications in biotechnology. However, the genetic bases of these capabilities remain unclear due largely to an absence of technologies that link DNA sequence to molecular function. To address this challenge, we are developing a pipeline for high throughput annotation of gene function using mutagenesis, growth assays and DNA sequencing. By applying this pipeline to annotate gene function in 50 diverse microbes we hope to discover thousands of new gene functions and produce a proof of principle `Functional Encyclopedia of Bacteria and Archaea?.

  11. Soft rot decay capabilities and interactions of fungi and bacteria from fumigated utility poles

    SciTech Connect (OSTI)

    Wang, C.J.K.; Worrall, J.J. . Coll. of Environmental Science and Forestry)

    1992-11-01

    The objectives were to (1) identify microfungi and bacterial associates isolated from fumigated southern pine poles from EPRI project RP 1471-72, (2) study the soft-rot capabilities of predominant fungi, and (3) study interactions among microorganisms in relation to wood decay. Methods for identification followed standard techniques using morphological and physiological criteria. Soft-rot by microfungi alone and with bacteria was determined as weight loss and anatomical examination of wood blocks using light microscopy and limited electron microscopy. Acinetobacter calcoaceticus was the predominant bacterium. Twenty-one species of microfungi were identified including four new species. A book entitled IDENTIFICATION MANUAL FOR FUNGI FROM UTILITY POLES IN THE EASTERN UNITED STATES was published. An improved soft-rot test was devised. Fifty-one of 84 species (60%) of microfungi from poles tested were soft-rot positive; that is much greater than previously reported. Three types of anatomical damage of wood of pine or birch caused by soft-rot fungi were described. Interaction tests showed that, in some cases, there was a strong synergism between bacteria and fungi in causing weight loss, but results were inconsistent. Although soft rot is often most apparent under conditions of very high moisture, intermediate moisture levels appear to be optimal, as with basidiomycete decayers.

  12. Soft rot decay capabilities and interactions of fungi and bacteria from fumigated utility poles. Final report

    SciTech Connect (OSTI)

    Wang, C.J.K.; Worrall, J.J.

    1992-11-01

    The objectives were to (1) identify microfungi and bacterial associates isolated from fumigated southern pine poles from EPRI project RP 1471-72, (2) study the soft-rot capabilities of predominant fungi, and (3) study interactions among microorganisms in relation to wood decay. Methods for identification followed standard techniques using morphological and physiological criteria. Soft-rot by microfungi alone and with bacteria was determined as weight loss and anatomical examination of wood blocks using light microscopy and limited electron microscopy. Acinetobacter calcoaceticus was the predominant bacterium. Twenty-one species of microfungi were identified including four new species. A book entitled IDENTIFICATION MANUAL FOR FUNGI FROM UTILITY POLES IN THE EASTERN UNITED STATES was published. An improved soft-rot test was devised. Fifty-one of 84 species (60%) of microfungi from poles tested were soft-rot positive; that is much greater than previously reported. Three types of anatomical damage of wood of pine or birch caused by soft-rot fungi were described. Interaction tests showed that, in some cases, there was a strong synergism between bacteria and fungi in causing weight loss, but results were inconsistent. Although soft rot is often most apparent under conditions of very high moisture, intermediate moisture levels appear to be optimal, as with basidiomycete decayers.

  13. Anaerobic metabolism of nitroaromatic compounds by sulfate-reducing and methanogenic bacteria

    SciTech Connect (OSTI)

    Boopathy, R.; Kulpa, C.F.

    1994-06-01

    Ecological observations suggest that sulfate-reducing and methanogenic bacteria might metabolize nitroaromatic compounds under anaerobic conditions if appropriate electron donors and electron acceptors are present in the environment, but this ability had not been demonstrated until recently. Most studies on the microbial metabolism of nitroaromatic compounds used aerobic microorganisms. In most cases no mineralization of nitroaromatics occurs, and only superficial modifications of the structures are reported. However, under anaerobic sulfate-reducing conditions, the nitroaromatic compounds reportedly undergo a series of reductions with the formation of amino compounds. For example, trinitrotoluene under sulfate-reducing conditions is reduced to triaminotoluene by the enzyme nitrite reductase, which is commonly found in many Desulfovibrio spp. The removal of ammonia from triaminotoluene is achieved by reductive deamination catalyzed by the enzyme reductive deaminase, with the production of ammonia and toluene. Some sulfate reducers can metabolize toluene to CO{sub 2}. Similar metabolic processes could be applied to other nitroaromatic compounds like nitrobenzene, nitrobenzoic acids, nitrophenols, and aniline. Many methanogenic bacteria can reduce nitroaromatic compounds to amino compounds. In this paper we review the anaerobic metabolic processes of nitroaromatic compounds under sulfate-reducing And methanogenic conditions.

  14. Biofuel from Bacteria and Sunlight: Shewanella as an Ideal Platform for Producing Hydrocarbons

    SciTech Connect (OSTI)

    None

    2010-01-01

    Broad Funding Opportunity Announcement Project: The University of Minnesota is developing clean-burning, liquid hydrocarbon fuels from bacteria. The University is finding ways to continuously harvest hydrocarbons from a type of bacteria called Shewanella by using a photosynthetic organism to constantly feed Shewanella the sugar it needs for energy and hydrocarbon production. The two organisms live and work together as a system. Using Shewanella to produce hydrocarbon fuels offers several advantages over traditional biofuel production methods. First, it eliminates many of the time-consuming and costly steps involved in growing plants and harvesting biomass. Second, hydrocarbon biofuels resemble current petroleum-based fuels and would therefore require few changes to the existing fuel refining and distribution infrastructure in the U.S.

  15. Natural Oil Production from Microorganisms: Bioprocess and Microbe Engineering for Total Carbon Utilization in Biofuel Production

    SciTech Connect (OSTI)

    2010-07-15

    Electrofuels Project: MIT is using carbon dioxide (CO2) and hydrogen generated from electricity to produce natural oils that can be upgraded to hydrocarbon fuels. MIT has designed a 2-stage biofuel production system. In the first stage, hydrogen and CO2 are fed to a microorganism capable of converting these feedstocks to a 2-carbon compound called acetate. In the second stage, acetate is delivered to a different microorganism that can use the acetate to grow and produce oil. The oil can be removed from the reactor tank and chemically converted to various hydrocarbons. The electricity for the process could be supplied from novel means currently in development, or more proven methods such as the combustion of municipal waste, which would also generate the required CO2 and enhance the overall efficiency of MIT’s biofuel-production system.

  16. Subsurface interactions of actinide species and microorganisms : implications for the bioremediation of actinide-organic mixtures.

    SciTech Connect (OSTI)

    Banaszak, J.E.; Reed, D.T.; Rittmann, B.E.

    1999-02-12

    By reviewing how microorganisms interact with actinides in subsurface environments, we assess how bioremediation controls the fate of actinides. Actinides often are co-contaminants with strong organic chelators, chlorinated solvents, and fuel hydrocarbons. Bioremediation can immobilize the actinides, biodegrade the co-contaminants, or both. Actinides at the IV oxidation state are the least soluble, and microorganisms accelerate precipitation by altering the actinide's oxidation state or its speciation. We describe how microorganisms directly oxidize or reduce actinides and how microbiological reactions that biodegrade strong organic chelators, alter the pH, and consume or produce precipitating anions strongly affect actinide speciation and, therefore, mobility. We explain why inhibition caused by chemical or radiolytic toxicities uniquely affects microbial reactions. Due to the complex interactions of the microbiological and chemical phenomena, mathematical modeling is an essential tool for research on and application of bioremediation involving co-contamination with actinides. We describe the development of mathematical models that link microbiological and geochemical reactions. Throughout, we identify the key research needs.

  17. Chemotactic selection of pollutant degrading soil bacteria (Patent...

    Office of Scientific and Technical Information (OSTI)

    RADIATION SOURCES; BACTERIA; EVALUATION; POLLUTANTS; BIODEGRADATION; SOILS; NUTRIENTS; COLONY FORMATION; INVENTIONS; SOIL CHEMISTRY; MINERALIZATION; LAND POLLUTION 540120; 053003; ...

  18. Living Direct: Order (2011-CE-1904)

    Broader source: Energy.gov [DOE]

    DOE ordered Living Direct, Inc. to pay a $6,000 civil penalty after finding Living Direct had failed to certify that certain models of dishwashers, refrigerator-freezers and freezers comply with the applicable energy conservation standards.

  19. Fuel Cells Go Live | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Go Live Fuel Cells Go Live An article written by Michael Ciotti for Green Power that originally appeared in the Fourth Quarter, Fall 2008 issue of The MHEDA Journal ...

  20. Engineering Biofuels from Photosynthetic Bacteria - Energy Innovation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Portal Biomass and Biofuels Biomass and Biofuels Find More Like This Return to Search Engineering Biofuels from Photosynthetic Bacteria Argonne National Laboratory Contact ANL About This Technology <em>Schematic of the overall approach including the invented method for production of co-factors and anchors as biofuel precursors.</em> Schematic of the overall approach including the invented method for production of co-factors and anchors as biofuel precursors. Technology Marketing

  1. Comparative evolution of the recA gene of surface and deep subsurface microorganisms (an evolutionary clock of intermediate rate). Final report

    SciTech Connect (OSTI)

    Miller, R.V.

    1998-04-01

    Because of the ability of the recA protein product to maintain both DNA integrity and increase genetic diversity, this gene may be essential to the survival of microorganisms following the damaging effects of numerous environmental stresses such as exposure to solar UV radiation, exposure to gamma radiation, starvation, and changing environments. While the various activities and amino-acid sequence of recA have been highly conserved among the eubacteria and archaea, little is known as to whether a strict structure-function relationship has been conserved. In other words, are the same regions of this highly plastic, functionally heterogeneous protein involved in the same catalytic capacities throughout the bacterial kingdom? While it is reasonable to assume that this type of conservation has also occurred, we felt it necessary to test the assumption by demonstrating that mutations in different genera of bacteria which eliminate similar functions (i.e., lead to similar phenotypes) are caused by changes in the amino-acid sequence in the same regions of their recA proteins. Therefore, we located the changes in nucleotide sequence in two recA mutants of P. aeruginosa which displayed mutant phenotypes in recombination and UV resistance. Our assumption was that if structure-function relationships held, these mutations would be found in areas already identified as essential for the function of the E. coli recA protein.

  2. Biochemistry and physiology of anaerobic bacteria

    SciTech Connect (OSTI)

    2000-05-18

    We welcome you to The Power of Anaerobes. This conference serves two purposes. One is to celebrate the life of Harry D. Peck, Jr.,who was born May 18, 1927 and would have celebrated his 73rd birthday at this conference. He died November 20, 1998. The second is to gather investigators to exchange views within the realm of anaerobic microbiology, an area in which tremendous progress has been seen during recent years. It is sufficient to mention discoveries of a new form of life (the archaea), hyper or extreme thermophiles, thermophilic alkaliphiles and anaerobic fungi. With these discoveries has come a new realization about physiological and metabolic properties of microorganisms, and this in turn has demonstrated their importance for the development, maintenance and sustenance of life on Earth.

  3. Live Fire Range Environmental Assessment

    SciTech Connect (OSTI)

    1993-08-01

    The Central Training Academy (CTA) is a DOE Headquarters Organization located in Albuquerque, New Mexico, with the mission to effectively and efficiently educate and train personnel involved in the protection of vital national security interests of DOE. The CTA Live Fire Range (LFR), where most of the firearms and tactical training occurs, is a complex separate from the main campus. The purpose of the proposed action is to expand the LFR to allow more options of implementing required training. The Department of Energy has prepared this Environmental Assessment (EA) for the proposed construction and operation of an expanded Live Fire Range Facility at the Central Training Academy in Albuquerque, New Mexico. Based on the analysis in the EA, DOE has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an environmental impact statement is not required and DOE is issuing this Finding of No Significant Impact (FONSI).

  4. Design and mechanism of action of a novel bacteria-selective antimicrobial peptide from the cell-penetrating peptide Pep-1

    SciTech Connect (OSTI)

    Zhu, W.L.; Lan Hongliang; Park, Il-Seon; Kim, Jae Il; Jin, H.Z.; Hahm, Kyung-Soo; Shin, S.Y. . E-mail: syshin@chosun.ac.kr

    2006-10-20

    Here, we report the successful design of a novel bacteria-selective antimicrobial peptide, Pep-1-K (KKTWWKTWWTKWSQPKKKRKV). Pep-1-K was designed by replacing Glu-2, Glu-6, and Glu-11 in the cell-penetrating peptide Pep-1 with Lys. Pep-1-K showed strong antibacterial activity against reference strains (MIC = 1-2 {mu}M) of Gram-positive and Gram-negative bacteria as well as against clinical isolates (MIC = 1-8 {mu}M) of methicillin-resistant Staphylococcus aureus and multidrug-resistant Pseudomonas aeruginosa. In contrast, Pep-1-K did not cause hemolysis of human erythrocytes even at 200 {mu}M. These results indicate that Pep-1-K may be a good candidate for antimicrobial drug development, especially as a topical agent against antibiotic-resistant microorganisms. Tryptophan fluorescence studies indicated that the lack of hemolytic activity of Pep-1-K correlated with its weak ability to penetrate zwitterionic phosphatidylcholine/cholesterol (10:1, w/w) vesicles, which mimic eukaryotic membranes. Furthermore, Pep-1-K caused little or no dye leakage from negatively charged phosphatidylethanolamine/phosphatidylglycerol (7:3, w/w) vesicles, which mimic bacterial membranes but had a potent ability to cause depolarization of the cytoplasmic membrane potential of intact S. aureus cells. These results suggested that Pep-1-K kills microorganisms by not the membrane-disrupting mode but the formation of small channels that permit transit of ions or protons but not molecules as large as calcein.

  5. Live: DOE Cyber Distinguished Speaker Series | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Live: DOE Cyber Distinguished Speaker Series Live: DOE Cyber Distinguished Speaker Series Live streaming video by Ustream HOW ACADEMIC INSTITUIONS ARE MEETING TODAY'S CYBER ...

  6. International symposium on cellular and molecular biology of phosphate and phosphorylated compounds in microorganisms: Proceedings

    SciTech Connect (OSTI)

    1993-12-31

    This report contains the abstracts of papers presented at the conference. Attention is focused on the following topics: regulation of phosphate metabolism in bacteria; structure-function of alkaline phosphatase; regulation of phosphate metabolism in yeast; transport of phosphate and phosphorylated compounds; and phosphate regulation in pathogenesis and secondary metabolism.

  7. Detection of phenols using engineered bacteria

    DOE Patents [OSTI]

    Wise, Arlene A.; Kuske, Cheryl R.; Terwilliger, Thomas C.

    2004-08-10

    Detection of phenols using engineered bacteria. A biosensor can be created by placing a reporter gene under control of an inducible promoter. The reporter gene produces a signal when a cognate transcriptional activator senses the inducing chemical. Creation of bacterial biosensors is currently restricted by limited knowledge of the genetic systems of bacteria that catabolize xenobiotics. By using mutagenic PCR to change the chemical specificity of the Pseudomonas species CF600 DmpR protein, the potential for engineering novel biosensors for detection of phenols has been demonstrated. DmpR, a well-characterized transcriptional activator of the P. CF600's dmp operon mediates growth on simple phenols. Transcription from Po, the promoter heading the dmp operon, is activated when the sensor domain of DmpR interacts with phenol and mono-substituted phenols. By altering the sensor domain of the DmpR, a group of DmpR derivatives that activate transcription of a Po-lacZ fusion in response to eight of the EPA's eleven priority pollutant phenols has been created. The assays and the sensor domain mutations that alter the chemical specificity of DmpR is described.

  8. Detection of phenols using engineered bacteria

    DOE Patents [OSTI]

    Wise, Arlene A.; Kuske, Cheryl R.; Terwilliger, Thomas C.

    2007-12-04

    Detection of phenols using engineered bacteria. A biosensor can be created by placing a reporter gene under control of an inducible promoter. The reporter gene produces a signal when a cognate transcriptional activator senses the inducing chemical. Creation of bacterial biosensors is currently restricted by limited knowledge of the genetic systems of bacteria that catabolize xenobiotics. By using mutagenic PCR to change the chemical specificity of the Pseudomonas species CF600 DmpR protein, the potential for engineering novel biosensors for detection of phenols has been demonstrated. DmpR, a well-characterized transcriptional activator of the P. CF600's dmp operon mediates growth on simple phenols. Transcription from Po, the promoter heading the dmp operon, is activated when the sensor domain of DmpR interacts with phenol and mono-substituted phenols. By altering the sensor domain of the DmpR, a group of DmpR derivatives that activate transcription of a Po-lacZ fusion in response to eight of the EPA's eleven priority pollutant phenols has been created. The assays and the sensor domain mutations that alter the chemical specificity of DmpR is described.

  9. Biofuel and chemical production by recombinant microorganisms via fermentation of proteinaceous biomass

    DOE Patents [OSTI]

    Liao, James C.; Cho, Kwang Myung; Yan, Yajun; Huo, Yixin

    2016-03-15

    Provided herein are metabolically modified microorganisms characterized by having an increased keto-acid flux when compared with the wild-type organism and comprising at least one polynucleotide encoding an enzyme that when expressed results in the production of a greater quantity of a chemical product when compared with the wild-type organism. The recombinant microorganisms are useful for producing a large number of chemical compositions from various nitrogen containing biomass compositions and other carbon sources. More specifically, provided herein are methods of producing alcohols, acetaldehyde, acetate, isobutyraldehyde, isobutyric acid, n-butyraldehyde, n-butyric acid, 2-methyl-1-butyraldehyde, 2-methyl-1-butyric acid, 3-methyl-1-butyraldehyde, 3-methyl-1-butyric acid, ammonia, ammonium, amino acids, 2,3-butanediol, 1,4-butanediol, 2-methyl-1,4-butanediol, 2-methyl-1,4-butanediamine, isobutene, itaconate, acetoin, acetone, isobutene, 1,5-diaminopentane, L-lactic acid, D-lactic acid, shikimic acid, mevalonate, polyhydroxybutyrate (PHB), isoprenoids, fatty acids, homoalanine, 4-aminobutyric acid (GABA), succinic acid, malic acid, citric acid, adipic acid, p-hydroxy-cinnamic acid, tetrahydrofuran, 3-methyl-tetrahydrofuran, gamma-butyrolactone, pyrrolidinone, n-methylpyrrolidone, aspartic acid, lysine, cadeverine, 2-ketoadipic acid, and/or S-adenosyl-methionine (SAM) from a suitable nitrogen rich biomass.

  10. Rural Living Canada Website | Open Energy Information

    Open Energy Info (EERE)

    Equivalent URI: cleanenergysolutions.orgcontentrural-living-canada-website Language: English Policies: "Deployment Programs,Regulations" is not in the list of possible...

  11. Lancaster Live/Work Townhome Prototype

    SciTech Connect (OSTI)

    2010-04-01

    This case study describes development of a prototype live-work townhome that is highly efficient at 45% energy savings (95% counting photovoltaic system).

  12. Live Broadcast on Quadrennial Energy Review

    Broader source: Energy.gov [DOE]

    Watch a live broadcast of the Quadrennial Energy Review public stakeholder meeting on Thursday, February 4, 2016 at 9:30 a.m. EST

  13. Extending the Operating Lives of Materials

    ScienceCinema (OSTI)

    Paul Jablonski

    2010-09-01

    Metallurgist Paul Jablonski discusses his role in developing processes that extend the operating temperatures and operating lives of materials used in energy applications.

  14. Living Walls | OpenEI Community

    Open Energy Info (EERE)

    systems and the new field of biomimicry. Biomimicry is the science of imitating nature to solve human design problems. The Living Wall concept takes the principles behind...

  15. SOME BACTERIA ARE ESPECIALLY TOUGH. For billions of

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    SOME BACTERIA ARE ESPECIALLY TOUGH. For billions of years, bacteria have evolved numerous mechanisms to protect themselves from toxic chemicals in their environment-some of which we humans now use as antibiotics. Applying their time-tested methods of thwarting chemical threats, these hardy microbes are responsible for nearly two million antibiotic- resistant infections annually in the United States. Bacteria have developed many types of defenses. Like layering for winter, some microbes wear

  16. Methane and Methanotrophic Bacteria as a Biotechnological Platform |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Methane and Methanotrophic Bacteria as a Biotechnological Platform Methane and Methanotrophic Bacteria as a Biotechnological Platform Breakout Session 2-B: New/Emerging Pathways Methane and Methanotrophic Bacteria as a Biotechnological Platform Dr. Lori Giver, Vice President of Biological Engineering, Calysta Energy, Inc. giver_bioenergy_2015.pdf (1.68 MB) More Documents & Publications CX-100166 Categorical Exclusion Determination Biobased Chemicals Landscape in

  17. Utilizing Bacteria for Sustainable Manufacturing of Low-Cost Nanoparticles

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy Utilizing Bacteria for Sustainable Manufacturing of Low-Cost Nanoparticles Utilizing Bacteria for Sustainable Manufacturing of Low-Cost Nanoparticles Utilizing Bacteria for Sustainable Manufacturing of Low Cost Nanoparticles - Chad Duty, Oak Ridge National Laboratory (1.94 MB) More Documents & Publications Sustainable Nanomaterials Workshop Integrating Environmental, Safety, and Quality Management System Audits Performance Analysis of Air-Source Variable Speed Heat

  18. Conversion of cellulose to ethanol by mesophilic bacteria. Progress report, July 15, 1983-February 15, 1985

    SciTech Connect (OSTI)

    Canale-Parola, E.

    1985-03-15

    Highlights of accomplishments during the period from July 1983 to February 1985 are summarized. Research has dealt primarily with strains of obligately anaerobic, mesophilic cellulolytic bacteria that we isolated from various natural environments. Eight strains (referred to as C strains) were isolated from mud of freshwater environments. As described in the previous progress report, the C strains represented a species of Clostridium that was different from other described species. The C strains fermented cellulose with formation of ethanol. They differed from thermophilic cellulolytic clostridia (e.g. Clostridium thermocellum) not only in growth temperature range, but also because they fermented xylan and pentoses with formation of ethanol. This result indicated that these mesophilic clostridia can convert to ethanol both cellulosic and hemicellulosic components of biomass. In contrast, monocultures of Clostridium thermocellum ferment only the cellulosic component of biomass. Furthermore, cellulose was degraded by the C strains at a rate comparable to that of thermophilic cellulolytic clostridia. These observations indicated that the mesophilic cellulolytic isolates constituted potentially useful microorganisms for ethanol production from biomass.

  19. Field experiences in on-line bacteria monitoring

    SciTech Connect (OSTI)

    Smart, J.; Pickthall, T.; Wright, T.G.

    1996-08-01

    The results of field testing for bacteria and related corrosion are presented for three crude oil and one gas pipeline. Large numbers of bacteria were usually found in the crude oil pipelines, but large bacteria populations did not result in accelerated corrosion in these lines. Crude oil pipeline corrosion was found to be most rapid in sediment deposits, which consisted of oil wet corrosion products, paraffins, and water. Both bacteria populations and corrosion rates were low in the gas pipeline studied, due to a high residual di-amine corrosion inhibitor content in the pipeline water.

  20. Rapid quantification of mutant fitness in diverse bacteria by...

    Office of Scientific and Technical Information (OSTI)

    Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons Citation Details In-Document Search Title: Rapid quantification of mutant...

  1. Argonne scientists use bacteria to power simple machines | Argonne...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    University and placed in the solution along with the common aerobic bacteria Bacillus subtilis. Andrey Sokolov of Princeton University and Igor Aronson from Argonne, along...

  2. Bacteria mix it up at the microscopic level | Argonne National...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Technology and now postdoctoral researcher at Princeton University, piled Bacillus subtillis bacteria into thin films to decode the physics that govern how they move....

  3. Evaluation of sulfur-reducing microorganisms for organic desulfurization. [Pyrococcus furiosus

    SciTech Connect (OSTI)

    Miller, K.W.

    1991-01-01

    Because of substantial portion of the sulfur in Illinois coal is organic, microbial desulfurization of sulfidic and thiophenic functionalities could hold great potential for completing pyritic sulfur removal. We are testing the hypothesis that organic sulfur can be reductively removed as H{sub 2}S through the activities of anaerobic microorganisms. Our objectives for this year include the following: (1) To obtain cultures that will reductively desulfurize thiophenic model compounds. In addition to crude oil enrichments begun last year, we sampled municipal sewage sludge. (2) To continue to work toward optimizing the activity of the DBDS-reducing cultures obtained during the previous year. (3) To expand coal desulfurization work to include other coals including Illinois Basin Coal 101 and a North Dakota lignite, which might be more susceptible to the dibenzyldisulfide reducing cultures due to its lower rank. (4) To address the problem of sulfide sorption, by investigating the sorption capacity of coals in addition to Illinois Basin Coal 108.

  4. Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity

    DOE Patents [OSTI]

    Premuzic, Eugene T.; Lin, Mow

    1996-02-20

    This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70.degree. C. to 90.degree. C., at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%.

  5. Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity

    DOE Patents [OSTI]

    Premuzic, E.T.; Lin, M.

    1996-02-20

    This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70 C to 90 C, at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%. 68 figs.

  6. How sulphate-reducing microorganisms cope with stress: Lessons from systems biology

    SciTech Connect (OSTI)

    Zhou, J.; He, Q.; Hemme, C.L.; Mukhopadhyay, A.; Hillesland, K.; Zhou, A.; He, Z.; Nostrand, J.D. Van; Hazen, T.C.; Stahl, D.A.; Wall, J.D.; Arkin, A.P.

    2011-04-01

    Sulphate-reducing microorganisms (SRMs) are a phylogenetically diverse group of anaerobes encompassing distinct physiologies with a broad ecological distribution. As SRMs have important roles in the biogeochemical cycling of carbon, nitrogen, sulphur and various metals, an understanding of how these organisms respond to environmental stresses is of fundamental and practical importance. In this Review, we highlight recent applications of systems biology tools in studying the stress responses of SRMs, particularly Desulfovibrio spp., at the cell, population, community and ecosystem levels. The syntrophic lifestyle of SRMs is also discussed, with a focus on system-level analyses of adaptive mechanisms. Such information is important for understanding the microbiology of the global sulphur cycle and for developing biotechnological applications of SRMs for environmental remediation, energy production, biocorrosion control, wastewater treatment and mineral recovery.

  7. Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels

    SciTech Connect (OSTI)

    Kuk Lee, Sung; Chou, Howard; Ham, Timothy S.; Soon Lee, Taek; Keasling, Jay D.

    2009-12-02

    The ability to generate microorganisms that can produce biofuels similar to petroleum-based transportation fuels would allow the use of existing engines and infrastructure and would save an enormous amount of capital required for replacing the current infrastructure to accommodate biofuels that have properties significantly different from petroleum-based fuels. Several groups have demonstrated the feasibility of manipulating microbes to produce molecules similar to petroleum-derived products, albeit at relatively low productivity (e.g. maximum butanol production is around 20 g/L). For cost-effective production of biofuels, the fuel-producing hosts and pathways must be engineered and optimized. Advances in metabolic engineering and synthetic biology will provide new tools for metabolic engineers to better understand how to rewire the cell in order to create the desired phenotypes for the production of economically viable biofuels.

  8. Potential of Diazorphic, Endophytic Bacteria Associated with Sugarcane for

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energycane Production | Department of Energy Potential of Diazorphic, Endophytic Bacteria Associated with Sugarcane for Energycane Production Potential of Diazorphic, Endophytic Bacteria Associated with Sugarcane for Energycane Production This presentation by Michal Gisham was given at the Symbiosis Conference. symbiosis_conference_grisham.pdf (5.18 MB) More Documents & Publications Symbiosis Biofeedstock Conference: Expanding Commercialization of Mutualistic Microbes to Increase

  9. Identification of bacteria synthesizing ribosomal RNA in response to uranium addition during biostimulation at the Rifle, CO Integrated Field Research site

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    McGuinness, Lora R.; Wilkins, Michael J.; Williams, Kenneth H.; Long, Philip E.; Kerkhof, Lee J.; Boyanov, Maxim I.

    2015-09-18

    Understanding which organisms are capable of reducing uranium at historically contaminated sites provides crucial information needed to evaluate treatment options and outcomes. One approach is determination of the bacteria which directly respond to uranium addition. In this research, uranium amendments were made to groundwater samples from a site of ongoing biostimulation with acetate. The active microbes in the planktonic phase were deduced by monitoring ribosomes production via RT-PCR. The results indicated several microorganisms were synthesizing ribosomes in proportion with uranium amendment up to 2 μM. Concentrations of U (VI) >2 μM were generally found to inhibit ribosome synthesis. Two activemore » bacteria responding to uranium addition in the field were close relatives of Desulfobacter postgateii and Geobacter bemidjiensis. Since RNA content often increases with growth rate, our findings suggest it is possible to rapidly elucidate active bacteria responding to the addition of uranium in field samples and provides a more targeted approach to stimulate specific populations to enhance radionuclide reduction in contaminated sites.« less

  10. Identification of bacteria synthesizing ribosomal RNA in response to uranium addition during biostimulation at the Rifle, CO Integrated Field Research site

    SciTech Connect (OSTI)

    McGuinness, Lora R.; Wilkins, Michael J.; Williams, Kenneth H.; Long, Philip E.; Kerkhof, Lee J.; Boyanov, Maxim I.

    2015-09-18

    Understanding which organisms are capable of reducing uranium at historically contaminated sites provides crucial information needed to evaluate treatment options and outcomes. One approach is determination of the bacteria which directly respond to uranium addition. In this research, uranium amendments were made to groundwater samples from a site of ongoing biostimulation with acetate. The active microbes in the planktonic phase were deduced by monitoring ribosomes production via RT-PCR. The results indicated several microorganisms were synthesizing ribosomes in proportion with uranium amendment up to 2 μM. Concentrations of U (VI) >2 μM were generally found to inhibit ribosome synthesis. Two active bacteria responding to uranium addition in the field were close relatives of Desulfobacter postgateii and Geobacter bemidjiensis. Since RNA content often increases with growth rate, our findings suggest it is possible to rapidly elucidate active bacteria responding to the addition of uranium in field samples and provides a more targeted approach to stimulate specific populations to enhance radionuclide reduction in contaminated sites.

  11. Metal Cycling by Bacteria: Moving Electrons Around

    SciTech Connect (OSTI)

    Nealson, Ken

    2009-07-06

    About 20 years ago, Shewanella oneidensis MR-1 was isolated from a manganese-rich lack in upstate New York, and subsequently shown to utilize solid forms of oxidized manganese or iron as an electron acceptor. Recent studies of metal-reducing bacterial have unveiled a number of unexpected properties of microbes that have enlarged our view of microbes and their role(s) in natural ecosystems. For example, the processes of metal reduction themselves are fundamental to the carbon cycle in many lakes and sediments, where iron and manganese account for the major portion of organic carbon oxidation in many sediments. On more modest spatial scales, iron and manganese reduction can be linked to the oxidation of a wide variety of carbon compounds, many of them recalcitrant and/or toxic. One remarkable property of metal reducers is their ability to reduce solid, often highly crystalline substrates such as iron and manganese oxides and oxyhydroxides. It is now clear that this is done via the utilization of enzymes located on the outer wall of the bacteria - enzymes that apparently interact directly with these solid substrates. Molecular and genomic studies combined have revealed the genes and protoeins responsible for these activities, and many facets of the regulation. This talk focuses on the general features and properties of these remarkable organisms that seem to communicate via electron transfer across a wide variety of soluable, insoluable, and even "inert" substrates, and the way that these processes may be mechanistically linked.

  12. Metal Cycling by Bacteria: Moving Electrons Around

    ScienceCinema (OSTI)

    Nealson, Ken

    2010-01-08

    About 20 years ago, Shewanella oneidensis MR-1 was isolated from a manganese-rich lack in upstate New York, and subsequently shown to utilize solid forms of oxidized manganese or iron as an electron acceptor. Recent studies of metal-reducing bacterial have unveiled a number of unexpected properties of microbes that have enlarged our view of microbes and their role(s) in natural ecosystems. For example, the processes of metal reduction themselves are fundamental to the carbon cycle in many lakes and sediments, where iron and manganese account for the major portion of organic carbon oxidation in many sediments. On more modest spatial scales, iron and manganese reduction can be linked to the oxidation of a wide variety of carbon compounds, many of them recalcitrant and/or toxic. One remarkable property of metal reducers is their ability to reduce solid, often highly crystalline substrates such as iron and manganese oxides and oxyhydroxides. It is now clear that this is done via the utilization of enzymes located on the outer wall of the bacteria - enzymes that apparently interact directly with these solid substrates. Molecular and genomic studies combined have revealed the genes and protoeins responsible for these activities, and many facets of the regulation. This talk focuses on the general features and properties of these remarkable organisms that seem to communicate via electron transfer across a wide variety of soluable, insoluable, and even "inert" substrates, and the way that these processes may be mechanistically linked.

  13. Monitoring sulfide and sulfate-reducing bacteria

    SciTech Connect (OSTI)

    Tanner, R.S.

    1995-12-31

    Simple yet precise and accurate methods for monitoring sulfate-reducing bacteria (SRB) and sulfide remain useful for the study of bacterial souring and corrosion. Test kits are available to measure sulfide in field samples. A more precise methylene blue sulfide assay for both field and laboratory studies is described here. Improved media, compared to that in API RP-38, for enumeration of SRB have been formulated. One of these, API-RST, contained cysteine (1.1 mM) as a reducing agent, which may be a confounding source of sulfide. While cysteine was required for rapid enumeration of SRB from environmental samples, the concentration of cysteine in medium could be reduced to 0.4 mM. It was also determined that elevated levels of yeast extract (>1 g/liter) could interfere with enumeration of SRB from environmental samples. The API-RST medium was modified to a RST-11 medium. Other changes in medium composition, in addition to reduction of cysteine, included reduction of the concentration of phosphate from 3.4 mM to 2.2 mM, reduction of the concentration of ferrous iron from 0.8 mM to 0.5 mM and preparation of a stock mineral solution to ease medium preparation. SRB from environmental samples could be enumerated in a week in this medium.

  14. A C. elegans-based foam for rapid on-site detection of residual live virus.

    SciTech Connect (OSTI)

    Negrete, Oscar A.; Branda, Catherine; Hardesty, Jasper O. E.; Tucker, Mark David; Kaiser, Julia N.; Kozina, Carol L.; Chirica, Gabriela S.

    2012-02-01

    In the response to and recovery from a critical homeland security event involving deliberate or accidental release of biological agents, initial decontamination efforts are necessarily followed by tests for the presence of residual live virus or bacteria. Such 'clearance sampling' should be rapid and accurate, to inform decision makers as they take appropriate action to ensure the safety of the public and of operational personnel. However, the current protocol for clearance sampling is extremely time-intensive and costly, and requires significant amounts of laboratory space and capacity. Detection of residual live virus is particularly problematic and time-consuming, as it requires evaluation of replication potential within a eukaryotic host such as chicken embryos. The intention of this project was to develop a new method for clearance sampling, by leveraging Sandia's expertise in the biological and material sciences in order to create a C. elegans-based foam that could be applied directly to the entire contaminated area for quick and accurate detection of any and all residual live virus by means of a fluorescent signal. Such a novel technology for rapid, on-site detection of live virus would greatly interest the DHS, DoD, and EPA, and hold broad commercial potential, especially with regard to the transportation industry.

  15. Living Direct: Proposed Penalty (2011-CE-1904)

    Broader source: Energy.gov [DOE]

    DOE alleged in a Notice of Proposed Civil Penalty that Living Direct, Inc. failed to certify a variety of dishwashers, refrigerator-freezers, and freezers as compliant with the applicable energy conservation standards.

  16. Method for sustaining microorganism culture in syngas fermentation process in decreased concentration or absence of various substrates

    DOE Patents [OSTI]

    Adams, Stephen S.; Scott, Syrona; Ko, Ching-Whan

    2015-05-19

    The present invention relates to methods for sustaining microorganism culture in a syngas fermentation reactor in decreased concentration or absence of various substrates comprising: adding carbon dioxide and optionally alcohol; maintaining free acetic acid concentrations; and performing the above mentioned steps within specified time.

  17. Spatially-Resolved Analysis of Glycolipids and Metabolites in Living Synechococcus sp. PCC7002 Using Nanospray Desorption Electrospray Ionization

    SciTech Connect (OSTI)

    Lanekoff, Ingela T.; Geydebrekht, Oleg V.; Pinchuk, Grigoriy E.; Konopka, Allan; Laskin, Julia

    2013-04-07

    Microorganisms release a diversity of organic compounds that couple interspecies metabolism, enable communication, or provide benefits to other microbes. Increased knowledge of microbial metabolite production will contribute to understanding of the dynamic microbial world and can potentially lead to new developments in drug discovery, biofuel production, and clinical research. Nanospray desorption electrospray ionization (nano-DESI) is an ambient ionization technique that enables detailed chemical characterization of molecules from a specific location on a surface without special sample pretreatment. Due to its ambient nature, living bacterial colonies growing on agar plates can be rapidly and non-destructively analyzed. We performed spatially resolved nano-DESI analysis of living Synechococcus sp. PCC 7002 colonies on agar plates. We use high resolution mass spectrometry and MS/MS analysis of the living Synechococcus sp. PCC 7002 colonies to detect metabolites and lipids, and confirm their identities. We found that despite the high salt content of the agar (osmolarity ca. 700 mM), nano-DESI analysis enables detailed characterization of metabolites produced by the colony. Using this technique, we identified several glycolipids found on the living colonies and examined the effect of the age of the colony on the chemical gradient of glucosylglycerol secreted onto agar.

  18. Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1997-12-16

    Bacteria which produce large amounts of cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  19. Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1998-05-26

    Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  20. Bioprocessing of lignite coals using reductive microorganisms. Final technical report, September 30, 1988--March 29, 1992

    SciTech Connect (OSTI)

    Crawford, D.L.

    1992-03-29

    In order to convert lignite coals into liquid fuels, gases or chemical feedstock, the macromolecular structure of the coal must be broken down into low molecular weight fractions prior to further modification. Our research focused on this aspect of coal bioprocessing. We isolated, characterized and studied the lignite coal-depolymerizing organisms Streptomyces viridosporus T7A, Pseudomonas sp. DLC-62, unidentified bacterial strain DLC-BB2 and Gram-positive Bacillus megaterium strain DLC-21. In this research we showed that these bacteria are able to solubilize and depolymerize lignite coals using a combination of biological mechanisms including the excretion of coal solublizing basic chemical metabolites and extracellular coal depolymerizing enzymes.

  1. Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig

    1997-12-16

    Bacteria which produce large amounts of cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  2. Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig

    1998-01-01

    Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  3. Natural analogue studies of the role of colloids, natural organics and microorganisms on radionuclide transport

    SciTech Connect (OSTI)

    McCarthy, J.F.

    1994-10-01

    Colloids may be important as a geochemical transport mechanism for radionuclides at geological repositories if they are (1) present in the groundwater, (2) stable with respect to both colloidal and chemical stabilities, (3) capable of adsorbing radionuclides, especially if the sorption is irreversible, and (4) mobile in the subsurface. The available evidence from natural analogue and other field studies relevant to these issues is reviewed, as is the potential role of mobile microorganisms ({open_quotes}biocolloids{close_quotes}) on radionuclide migration. Studies have demonstrated that colloids are ubiquitous in groundwater, although colloid concentrations in deep, geochemically stable systems may be too low to affect radionuclide transport. However, even low colloid populations cannot be dismissed as a potential concern because colloids appear to be stable, and many radionuclides that adsorb to colloids are not readily desorbed over long periods. Field studies offer somewhat equivocal evidence concerning colloid mobility and cannot prove or disprove the significance of colloid transport in the far-field environment. Additional research is needed at new sites to properly represent a repository far-field. Performance assessment would benefit from natural analogue studies to examine colloid behavior at sites encompassing a suite of probable groundwater chemistries and that mimic the types of formations selected for radioactive waste repositories.

  4. Small Talk: Cell-to-Cell Communication in Bacteria

    ScienceCinema (OSTI)

    Bassler, Bonnie [Princeton University, Princeton, New Jersey, United States

    2010-01-08

    Cell-cell communication in bacteria involves the production, release, and subsequent detection of chemical signaling molecules called autoinducers. This process, called quorum sensing, allows bacteria to regulate gene expression on a population-wide scale. Processes controlled by quorum sensing are usually ones that are unproductive when undertaken by an individual bacterium but become effective when undertaken by the group. For example, quorum sensing controls bioluminescence, secretion of virulence factors, biofilm formation, sporulation, and the exchange of DNA. Thus, quorum sensing is a mechanism that allows bacteria to function as multi-cellular organisms. Bacteria make, detect, and integrate information from multiple autoinducers, some of which are used exclusively for intra-species communication while others enable communication between species. Research is now focused on the development of therapies that interfere with quorum sensing to control bacterial virulence.

  5. Utilizing Bacteria for Sustainable Manufacturing of Low-Cost...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bacteria for Sustainable Manufacturing of Low-Cost Nanoparticles Chad Duty, Ph.D. Technical Lead Additive Manufacturing Roll-to-Roll Processing June 26, 2012 2 Managed by ...

  6. Multiple Species of Bacteria Convert Elemental Mercury to Toxic...

    Office of Science (SC) Website

    Researchers are studying how bacteria transform mercury into a toxic form in the environment that can accumulate in the food web, posing a threat to wildlife and people. The ...

  7. Material and method for promoting the growth of anaerobic bacteria

    DOE Patents [OSTI]

    Adler, Howard I.

    1984-01-01

    A material and method for promoting the growth of anaerobic bacteria which includes a nutrient media containing a hydrogen donor and sterile membrane fragments of bacteria having an electron transfer system which reduces oxygen to water. Dissolved oxygen in the medium is removed by adding the sterile membrane fragments to the nutrient medium and holding the medium at a temperature of about 10.degree. to about 60.degree. C. until the dissolved oxygen is removed.

  8. Material and method for promoting the growth of anaerobic bacteria

    DOE Patents [OSTI]

    Adler, H.I.

    1984-10-09

    A material and method is disclosed for promoting the growth of anaerobic bacteria which includes a nutrient media containing a hydrogen donor and sterile membrane fragments of bacteria having an electron transfer system which reduces oxygen to water. Dissolved oxygen in the medium is removed by adding the sterile membrane fragments to the nutrient medium and holding the medium at a temperature of about 10 to about 60 C until the dissolved oxygen is removed. No Drawings

  9. Senior Adviser Richard Kauffman to Host Live Chat on Renewable...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and Deployment Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and...

  10. HMPT: Hazardous Waste Transportation Live 27928, Test 27929 ...

    Office of Scientific and Technical Information (OSTI)

    HMPT: Hazardous Waste Transportation Live 27928, Test 27929 Citation Details In-Document Search Title: HMPT: Hazardous Waste Transportation Live 27928, Test 27929 You are ...

  11. HMPT: Hazardous Waste Transportation Live 27928, Test 27929 ...

    Office of Scientific and Technical Information (OSTI)

    Technical Report: HMPT: Hazardous Waste Transportation Live 27928, Test 27929 Citation Details In-Document Search Title: HMPT: Hazardous Waste Transportation Live 27928, Test 27929 ...

  12. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Tracking Living Cells as They Differentiate in Real Time Print Thursday, 27 September 2012 00:00 Protein phosphorylation ...

  13. JV between KP Renewables and Living Buildings | Open Energy Informatio...

    Open Energy Info (EERE)

    KP Renewables and Living Buildings Jump to: navigation, search Name: JV between KP Renewables and Living Buildings Place: Brentford, Middlesex, Greater London, United Kingdom Zip:...

  14. Influence of microorganisms on the oxidation state distribution of multivalent actinides under anoxic conditions

    SciTech Connect (OSTI)

    Reed, Donald Timothy; Borkowski, Marian; Lucchini, Jean - Francois; Ams, David; Richmann, M. K.; Khaing, H.; Swanson, J. S.

    2010-12-10

    The fate and potential mobility of multivalent actinides in the subsurface is receiving increased attention as the DOE looks to cleanup the many legacy nuclear waste sites and associated subsurface contamination. Plutonium, uranium and neptunium are the near-surface multivalent contaminants of concern and are also key contaminants for the deep geologic disposal of nuclear waste. Their mobility is highly dependent on their redox distribution at their contamination source as well as along their potential migration pathways. This redox distribution is often controlled, especially in the near-surface where organic/inorganic contaminants often coexist, by the direct and indirect effects of microbial activity. Under anoxic conditions, indirect and direct bioreduction mechanisms exist that promote the prevalence of lower-valent species for multivalent actinides. Oxidation-state-specific biosorption is also an important consideration for long-term migration and can influence oxidation state distribution. Results of ongoing studies to explore and establish the oxidation-state specific interactions of soil bacteria (metal reducers and sulfate reducers) as well as halo-tolerant bacteria and Archaea for uranium, neptunium and plutonium will be presented. Enzymatic reduction is a key process in the bioreduction of plutonium and uranium, but co-enzymatic processes predominate in neptunium systems. Strong sorptive interactions can occur for most actinide oxidation states but are likely a factor in the stabilization of lower-valent species when more than one oxidation state can persist under anaerobic microbiologically-active conditions. These results for microbiologically active systems are interpreted in the context of their overall importance in defining the potential migration of multivalent actinides in the subsurface.

  15. Live pathogens: rapid detection technique developed

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Live Discussion on Energy 101: Fuel Cells Live Discussion on Energy 101: Fuel Cells January 16, 2014 - 3:59pm Addthis Rebecca Matulka Rebecca Matulka Former Digital Communications Specialist, Office of Public Affairs Editor's Note: Thanks to everyone who participated in our Google+ Hangout on Energy 101: Fuel Cells. We got a lot of great questions, and our experts talked about everything from the future of fuel cell vehicles and how they're being used as backup power to the efficiency benefits

  16. An Approach for Assessing the Signature Quality of Various Chemical Assays when Predicting the Culture Media Used to Grow Microorganisms

    SciTech Connect (OSTI)

    Holmes, Aimee E.; Sego, Landon H.; Webb-Robertson, Bobbie-Jo M.; Kreuzer, Helen W.; Anderson, Richard M.; Unwin, Stephen D.; Weimar, Mark R.; Tardiff, Mark F.; Corley, Courtney D.

    2013-02-01

    We demonstrate an approach for assessing the quality of a signature system designed to predict the culture medium used to grow a microorganism. The system was comprised of four chemical assays designed to identify various ingredients that could be used to produce the culture medium. The analytical measurements resulting from any combination of these four assays can be used in a Bayesian network to predict the probabilities that the microorganism was grown using one of eleven culture media. We evaluated combinations of the signature system by removing one or more of the assays from the Bayes network. We measured and compared the quality of the various Bayes nets in terms of fidelity, cost, risk, and utility, a method we refer to as Signature Quality Metrics

  17. Biodesulfurization techniques: Application of selected microorganisms for organic sulfur removal from coals. Final report

    SciTech Connect (OSTI)

    Elmore, B.B.

    1993-08-01

    As an alternative to post-combustion desulfurization of coal and pre-combustion desulfurization using physicochemical techniques, the microbial desulfurization of coal may be accomplished through the use of microbial cultures that, in an application of various microbial species, may remove both the pyritic and organic fractions of sulfur found in coal. Organisms have been isolated that readily depyritize coal but often at prohibitively low rates of desulfurization. Microbes have also been isolated that may potentially remove the organic-sulfur fraction present in coal (showing promise when acting on organic sulfur model compounds such as dibenzothiophene). The isolation and study of microorganisms demonstrating a potential for removing organic sulfur from coal has been undertaken in this project. Additionally, the organisms and mechanisms by which coal is microbially depyritized has been investigated. Three cultures were isolated that grew on dibenzothiophene (DBT), a model organic-sulfur compound, as the sole sulfur source. These cultures (UMX3, UMX9, and IGTS8) also grew on coal samples as the sole sulfur source. Numerous techniques for pretreating and ``cotreating`` coal for depyritization were also evaluated for the ability to improve the rate or extent of microbial depyritization. These include prewashing the coal with various solvents and adding surfactants to the culture broth. Using a bituminous coal containing 0.61% (w/w) pyrite washed with organic solvents at low slurry concentrations (2% w/v), the extent of depyritization was increased approximately 25% in two weeks as compared to controls. At slurry concentrations of 20% w/v, a tetrachloroethylene treatment of the coal followed by depyritization with Thiobacillus ferrooxidans increased both the rate and extent of depyritization by approximately 10%.

  18. Live Webinar on Better Buildings Challenge: Public-Sector Update

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webinar titled "Better Buildings Challenge: Public-Sector Update."

  19. Bacteria transport through porous media. Annual report, December 31, 1984

    SciTech Connect (OSTI)

    Yen, T.F.

    1986-09-01

    The following five chapters in this report have been processed separately for inclusion in the Energy Data Base: (1) theoretical model of convective diffusion of motile and non-motile bacteria toward solid surfaces; (2) interfacial electrochemistry of oxide surfaces in oil-bearing sands and sandstones; (3) effects of sodium pyrophosphate additive on the ''huff and puff''/nutrient flooding MEOR process; (4) interaction of Escherichia coli B, B/4, and bacteriophage T4D with Berea sandstone rock in relation to enhanced oil recovery; and (5) transport of bacteria in porous media and its significance in microbial enhanced oil recovery.

  20. Hairlike appendages on ultra-small bacteria cell

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hairlike appendages on ultra-small bacteria cell Click to share on Facebook (Opens in new window) Click to share on Twitter (Opens in new window) Click to share on Reddit (Opens in new window) Click to share on Pinterest (Opens in new window) Cryo-transmission electron microscopy captured numerous hairlike appendages radiating from the surface of this ultra-small bacteria cell. The scientists theorize the pili-like structures enable the cell to connect with other microbes and obtain life-giving

  1. Fission barriers and half-lives

    SciTech Connect (OSTI)

    Moeller, P.; Nix, J.R.; Swiatecki, W.J.

    1989-01-01

    We briefly review the development of theoretical models for the calculation of fission barriers and half-lives. We focus on how results of actual calculations in a unified macroscopic-microscopic approach provide an interpretation of the mechanisms behind some of the large number of phenomena observed in fission. As instructive examples we choose studies of the rapidly varying fission properties of elements at the end of the periodic system. 31 refs., 10 figs.

  2. Method for establishing the presence of salmonella bacteria in eggs

    DOE Patents [OSTI]

    Johnston, Roger G.; Sinha, Dipen N.

    1995-01-01

    Measurement of the acoustical resonances in eggs is shown to provide a rapid, noninvasive technique for establishing the presence of Salmonella bacteria. The technique is also sensitive to yolk puncture, shell cracks, and may be sensitive to other yolk properties and to egg freshness. Remote characterization, potentially useful for characterizing large numbers of eggs, has been demonstrated.

  3. Methods for targetted mutagenesis in gram-positive bacteria

    DOE Patents [OSTI]

    Yang, Yunfeng

    2014-05-27

    The present invention provides a method of targeted mutagenesis in Gram-positive bacteria. In particular, the present invention provides a method that effectively integrates a suicide integrative vector into a target gene in the chromosome of a Gram-positive bacterium, resulting in inactivation of the target gene.

  4. New Advances in Optical Imaging of Live Cells and Organisms

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New Advances in Optical Imaging of Live Cells and Organisms New Advances in Optical Imaging of Live Cells and Organisms Print http://csh-asia.com/13image.html August 20-23, 2913; Suzhou, China

  5. When Saving Energy Helps Save Lives | Department of Energy

    Energy Savers [EERE]

    When Saving Energy Helps Save Lives When Saving Energy Helps Save Lives May 3, 2016 - 2:41pm Addthis The US Army Corps of Engineers Hut test site in Champaign, IL. ORNL used ...

  6. New Advances in Optical Imaging of Live Cells and Organisms

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in Optical Imaging of Live Cells and Organisms New Advances in Optical Imaging of Live Cells and Organisms Print http:csh-asia.com13image.html August 20-23, 2913; Suzhou, China...

  7. Hanwha L C Hanwha Living and Creative | Open Energy Information

    Open Energy Info (EERE)

    Hanwha L C Hanwha Living and Creative Jump to: navigation, search Name: Hanwha L&C (Hanwha Living and Creative) Place: Seoul, Seoul, Korea (Republic) Zip: 100-797 Sector: Solar...

  8. DOE Tour of Zero Floorplans: Options for Community Living by...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Options for Community Living by United Way of Long Island DOE Tour of Zero Floorplans: Options for Community Living by United Way of Long Island DOE Tour of Zero Floorplans: ...

  9. Today: Live from the Carbon Capture and Storage Forum | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Today: Live from the Carbon Capture and Storage Forum Today: Live from the Carbon Capture and Storage Forum September 8, 2010 - 10:10am Addthis John Schueler John Schueler Former ...

  10. Safety assessment of outdoor live fire range

    SciTech Connect (OSTI)

    1989-05-01

    The following Safety Assessment (SA) pertains to the outdoor live fire range facility (LFR). The purpose of this facility is to supplement the indoor LFR. In particular it provides capacity for exercises that would be inappropriate on the indoor range. This SA examines the risks that are attendant to the training on the outdoor LFR. The outdoor LFR used by EG&G Mound is privately owned. It is identified as the Miami Valley Shooting Grounds. Mondays are leased for the exclusive use of EG&G Mound.

  11. A vast collection of microbial genes that are toxic to bacteria...

    Office of Scientific and Technical Information (OSTI)

    A vast collection of microbial genes that are toxic to bacteria Citation Details In-Document Search Title: A vast collection of microbial genes that are toxic to bacteria In the ...

  12. Toxin-eating bacteria and bioremediation (Patent) | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Toxin-eating bacteria and bioremediation Citation Details In-Document Search Title: Toxin-eating bacteria and bioremediation Methods are provided for reducing a level of one or ...

  13. MamO Is a Repurposed Serine Protease that Promotes Magnetite Biomineralization through Direct Transition Metal Binding in Magnetotactic Bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hershey, David M.; Ren, Xuefeng; Melnyk, Ryan A.; Browne, Patrick J.; Ozyamak, Ertan; Jones, Stephanie R.; Chang, Michelle C. Y.; Hurley, James H.; Komeili, Arash

    2016-03-16

    Many living organisms transform inorganic atoms into highly ordered crystalline materials. An elegant example of such biomineralization processes is the production of nano-scale magnetic crystals in magnetotactic bacteria. Previous studies have implicated the involvement of two putative serine proteases, MamE and MamO, during the early stages of magnetite formation in Magnetospirillum magneticum AMB-1. Here, using genetic analysis and X-ray crystallography, we show that MamO has a degenerate active site, rendering it incapable of protease activity. Instead, MamO promotes magnetosome formation through two genetically distinct, noncatalytic activities: activation of MamE-dependent proteolysis of biomineralization factors and direct binding to transition metal ions.more » By solving the structure of the protease domain bound to a metal ion, we identify a surface-exposed di-histidine motif in MamO that contributes to metal binding and show that it is required to initiate biomineralization in vivo. Finally, we find that pseudoproteases are widespread in magnetotactic bacteria and that they have evolved independently in three separate taxa. In conclusion, our results highlight the versatility of protein scaffolds in accommodating new biochemical activities and provide unprecedented insight into the earliest stages of biomineralization.« less

  14. Secret Lives of Scientists: Matt Kwiatkowski | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Secret Lives of Scientists: Matt Kwiatkowski Secret Lives of Scientists &amp; Engineers: Matt Kwiatkowski 1 of 16 Secret Lives of Scientists & Engineers: Matt Kwiatkowski Matt Kwiatkowski is a cyber security manager at Argonne National Lab - and flies over it in a plane he built himself during the weekends. Read the full story &raquo; Photos by Mark Lopez/Argonne National Laboratory. 31296D Secret Lives of Scientists &amp; Engineers: Matt Kwiatkowski 1 of 16 Secret Lives of

  15. Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio

    SciTech Connect (OSTI)

    Chhabra, Swapnil R; Keller, Kimberly L.; Wall, Judy D.

    2011-03-15

    Sulfate reducing bacteria are physiologically important given their nearly ubiquitous presence and have important applications in the areas of bioremediation and bioenergy. This chapter provides details on the steps used for homologous-recombination mediated chromosomal manipulation of Desulfovibrio vulgaris Hildenborough, a well-studied sulfate reducer. More specifically, we focus on the implementation of a 'parts' based approach for suicide vector assembly, important aspects of anaerobic culturing, choices for antibiotic selection, electroporation-based DNA transformation, as well as tools for screening and verifying genetically modified constructs. These methods, which in principle may be extended to other sulfate-reducing bacteria, are applicable for functional genomics investigations, as well as metabolic engineering manipulations.

  16. Universal Gene Transfer Technology for Gram Positive Bacteria - Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Innovation Portal Biomass and Biofuels Biomass and Biofuels Advanced Materials Advanced Materials Find More Like This Return to Search Universal Gene Transfer Technology for Gram Positive Bacteria Oak Ridge National Laboratory Contact ORNL About This Technology Publications: PDF Document Publication 11-G00255_ID2139_rev.pdf (493 KB) Technology Marketing SummaryA genetic engineering technology invented at ORNL facilitates DNA delivery to a cell by using ultrasound to permeate the cell's

  17. Apparatus and method for transforming living cells (Patent) ...

    Office of Scientific and Technical Information (OSTI)

    the cell walls or membrane of host cells one at a time so that a particular substance (e.g. a molecular tag, nucleic acid, bacteria, virus etc.) can be introduced into the cell. ...

  18. Evaluation of sulfur-reducing microorganisms for organic desulfurization. Final technical report, September 1, 1990--August 31, 1991

    SciTech Connect (OSTI)

    Miller, K.W.

    1991-12-31

    Because of substantial portion of the sulfur in Illinois coal is organic, microbial desulfurization of sulfidic and thiophenic functionalities could hold great potential for completing pyritic sulfur removal. We are testing the hypothesis that organic sulfur can be reductively removed as H{sub 2}S through the activities of anaerobic microorganisms. Our objectives for this year include the following: (1) To obtain cultures that will reductively desulfurize thiophenic model compounds. In addition to crude oil enrichments begun last year, we sampled municipal sewage sludge. (2) To continue to work toward optimizing the activity of the DBDS-reducing cultures obtained during the previous year. (3) To expand coal desulfurization work to include other coals including Illinois Basin Coal 101 and a North Dakota lignite, which might be more susceptible to the dibenzyldisulfide reducing cultures due to its lower rank. (4) To address the problem of sulfide sorption, by investigating the sorption capacity of coals in addition to Illinois Basin Coal 108.

  19. Engineering Bacteria for Efficient Fuel Production: Novel Biological Conversion of Hydrogen and Carbon Dioxide Directly into Free Fatty Acids

    SciTech Connect (OSTI)

    2010-07-12

    Electrofuels Project: OPX Biotechnologies is engineering a microorganism currently used in industrial biotechnology to directly produce a liquid fuel from hydrogen and carbon dioxide (CO2). The microorganism has the natural ability to use hydrogen and CO2 for growth. OPX Biotechnologies is modifying the microorganism to divert energy and carbon away from growth and towards the production of liquid fuels in larger, commercially viable quantities. The microbial system will produce a fuel precursor that can be chemically upgraded to various hydrocarbon fuels.

  20. Electromicroinjection of particles into living cells

    DOE Patents [OSTI]

    Ray, F. Andrew; Cram, L. Scott; Galey, William R.

    1988-01-01

    Method and apparatus for introducing particles into living cells. Fluorescently-stained human chromosomes are introduced into cultured, mitotic Chinese hamster cells using electromicroinjection. The recipient cells frequently survived the physiological perturbation imposed by a successful chromosome injection. Successfully injected recipient cells maintained viability as evidenced by their ability to be expanded. The technique relies on the surface charge of fluorescently stained chromosomes and their ability to be attracted and repelled to and from the tip of a micropipette. The apparatus includes a micropipette having a tip suitable for piercing the membrane of a target cell and an electrode inserted into the lumen thereof. The target cells and suspended particles are located in an electrically conducted solution, and the lumen of the micropipette is filled with an electrically conducting solution which contacts the electrode located therein. A second electrode is also located in the conducting solution containing the target cells and particles. Voltages applied to the electrode within the micropipette attract the particles to the region of the tip thereof. The particles adhere to the surface of the micropipette with sufficient force that insertion of the micropipette tip and attached particle through the membrane of a target cell will not dislodge the particle. By applying a voltage having the opposite polarity of the attraction voltage, the particles are expelled from the micropipette to which is then withdrawn from the cell body.

  1. HMPT: Hazardous Waste Transportation Live 27928, Test 27929 (Technical

    Office of Scientific and Technical Information (OSTI)

    Report) | SciTech Connect HMPT: Hazardous Waste Transportation Live 27928, Test 27929 Citation Details In-Document Search Title: HMPT: Hazardous Waste Transportation Live 27928, Test 27929 HMPT: Hazardous Waste Transportation (Live 27928, suggested one time and associated Test 27929, required initially and every 36 months) addresses the Department of Transportation (DOT) function-specific training requirements of the hazardous materials packagings and transportation (HMPT) Los Alamos

  2. Live webcast on groundbreaking results of Algal Biofuels Consortium, June

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    11 Live webcast on Algal Biofuels Consortium Live webcast on groundbreaking results of Algal Biofuels Consortium, June 11 Jose A. Olivares will present an overview of the technologies and processes that are needed to widely produce algae-based liquid transportation fuels. June 10, 2014 Jose A. Olivares Jose A. Olivares Contact Nancy Ambrosiano Communications Office (505) 667-0471 Email The Energy Department will present a live webcast titled "Algal Biofuels Consortium Releases

  3. National Clean Energy Business Plan Competition: Living Ink Technologies

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Wins CU Clean Energy Competition Regional Championship | Department of Energy Living Ink Technologies Wins CU Clean Energy Competition Regional Championship National Clean Energy Business Plan Competition: Living Ink Technologies Wins CU Clean Energy Competition Regional Championship May 4, 2015 - 2:51pm Addthis Living Ink Technologies has developed a patent-pending technology that uses algae to transform carbon dioxide into ink that is cheaper, healthier, and more environmentally

  4. Elimination of mercury and organomercurials by nitrogen-fixing bacteria

    SciTech Connect (OSTI)

    Ghosh, S.; Sadhukhan, P.C.; Ghosh, D.K.

    1997-06-01

    Bacteria isolated from mercury-polluted environments are often resistant to mercuric ions (Hg{sup 2+}) and organomercurials. Plasmids determining mercury resistance have been well characterized in gram-negative system. However, in Staphylococcus aureus mercury resistance has been found to be chromosomally determined. The known mechanism of bacterial Hg{sup 2+}-resistance is detoxification of the toxic Hg{sup 2+} by its enzymatic transformation by mercuric reductase to Hg (o). Organomercurial lyase mediates the degradation of organomercurial compounds to Hg{sup 2+}. Mercury and organomercurial resistances have been studied in different bacterial genera. There is little information on Hg-resistance in N{sub 2}-fixing soil bacteria, however, in many developing countries, including India, mercury pollution is still a problem because Hg-based pesticides and fungicides are still used routinely as seed-dressers in agriculture to control soil-borne and seed-borne fungal diseases. Volatilization of Hg from laboratory media by mercury-resistant bacteria containing low levels of mercury has been reported by several workers. It is interesting to note that N{sub 2}-fixing, Hg-resistant soil isolates could volatilize Hg from medium containing very high amounts of HgCl{sub 2}. In the present paper we report the volatilization patterns of five N{sub 2}-fixing bacterial strains, the effect of different inducers on mercuric reductase, and the pattern of substrate utilization by organomercurial lyase. In the presence of a low concentration of HgCl{sub 2}. enzymatic detoxification is sufficient to combat the adverse situation created by the presence of Hg{sup 2+} ions. In the presence of a high concentration of HgCl{sub 2}, intracellular sequestration by Hg{sup 2+} binding components may play an additional role in counteracting Hg-toxicity.

  5. New Advances in Optical Imaging of Live Cells and Organisms

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New Advances in Optical Imaging of Live Cells and Organisms http://csh-asia.com/13image.html August 20-23, 2913; Suzhou, China

  6. Table HC1.2.1. Living Space Characteristics by

    U.S. Energy Information Administration (EIA) Indexed Site

    Space Characteristics by" " Total, Heated, and Cooled Floorspace, 2005" ,,,"Total Square Footage" ,"Housing Units",,"Total1",,"Heated",,"Cooled" "Living Space Characteristics","Mil...

  7. The living publication (Technical Report) | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    The first article describes the present state of the crystallographic living publication, already recognized by an ALPSP (Association of Learned and Professional Society ...

  8. Spontaneous fission half-lives for ground state nuclides

    SciTech Connect (OSTI)

    Holden, N.E. [Brookhaven National Lab., Upton, NY (United States); Hoffman, D.C. [Lawrence Berkeley Lab., CA (United States)

    1993-08-01

    Measurements of the spontaneous fission half-lives of nuclides of elements Z = 90 to 108 have been compiled and evaluated. Recommended values are presented.

  9. Dutch Company Powers Streetlights With Living Plants; Will Your...

    Open Energy Info (EERE)

    Dutch Company Powers Streetlights With Living Plants; Will Your Cell Phone Be Next? Home > Groups > OpenEI Community Central Dc's picture Submitted by Dc(266) Contributor 16...

  10. National Clean Energy Business Plan Competition: Living Ink Technologi...

    Energy Savers [EERE]

    ... After winning the CU Clean Energy Competition Regional Championship, Living Ink Technologies will now join FGC Plasma Solutions, winner of the Clean Energy Challenge, and Hyliion, ...

  11. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation ... cells, enabling them to follow cellular chemical changes in real time, without bias. ...

  12. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins...

  13. Superior Energy Performance: New Website Live and Ready for Business...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Superior Energy Performance: New Website Live and Ready for Business January 16, 2014 - ... who want to prove their achievements in energy management and lower plant energy costs. ...

  14. Brownsville Public Utilities Board- Green Living Residential Rebate Program

    Broader source: Energy.gov [DOE]

     Brownsville Public Utilities Board offers residential customers rebates for installation of energy efficient measures. Through the Green Living Rebate program, customers can apply for rebates for...

  15. Genetics of Bacteria That Oxidize On-Carbon Compounds

    SciTech Connect (OSTI)

    Hanson, Richard S.

    2001-01-01

    Facultative methanol oxidizing bacteria contain large amounts of methanol dehydrogenase which is expressed only in the presence of methanol. This technical report describes two-two component regulatory systems encoding histidine kinases and response regulators and another response regulator all of which are required for the expression of mxaF, the open reading frame encoding methanol dehydrogenase. The response regulators bind to sequences upstream of the mxaF when phosphoryled in a reaction catalyzed by the histidine kinases. The binding of the response regulators is required for the transcription of mxaF.

  16. Detergent composition comprising a cellulase containing cell-free fermentate produced from microorganism ATCC 55702 or mutant thereof

    DOE Patents [OSTI]

    Dees, H.C.

    1998-07-14

    Bacteria which produce large amounts of a cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  17. Detergent composition comprising a cellulase containing cell-free fermentate produced from microorganism ATCC 55702 or mutant thereof

    DOE Patents [OSTI]

    Dees, H. Craig

    1998-01-01

    Bacteria which produce large amounts of a cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  18. Pathway of Fermentative Hydrogen Production by Sulfate-reducing Bacteria

    SciTech Connect (OSTI)

    Wall, Judy D.

    2015-02-16

    Biofuels are a promising source of sustainable energy. Such biofuels are intermediate products of microbial metabolism of renewable substrates, in particular, plant biomass. Not only are alcohols and solvents produced in this degradative process but energy-rich hydrogen as well. Non photosynthetic microbial hydrogen generation from compounds other than sugars has not been fully explored. We propose to examine the capacity of the abundant soil anaerobes, sulfate-reducing bacteria, for hydrogen generation from organic acids. These apparently simple pathways have yet to be clearly established. Information obtained may facilitate the exploitation of other microbes not yet readily examined by molecular tools. Identification of the flexibility of the metabolic processes to channel reductant to hydrogen will be useful in consideration of practical applications. Because the tools for genetic and molecular manipulation of sulfate-reducing bacteria of the genus Desulfovibrio are developed, our efforts will focus on two strains, D. vulgaris Hildenborough and Desulfovibrio G20.Therefore total metabolism, flux through the pathways, and regulation are likely to be limiting factors which we can elucidate in the following experiments.

  19. Harnessing the Sun: Energy in Our Lives | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Harnessing the Sun: Energy in Our Lives Harnessing the Sun: Energy in Our Lives Addthis Innovation Workshop: Harnessing the Sun highlights the science and engineering of how radiant energy is transformed into other useful forms of energy. Watch the rest of the videos

  20. Evidence of radicals created by plasma in bacteria in water

    SciTech Connect (OSTI)

    Lee, Chae Bok; Na, Young Ho; Hong, Tae-Eun; Choi, Eun Ha; Uhm, Han S.; Baik, Ku Youn E-mail: gckwon@kw.ac.kr; Kwon, Gichung E-mail: gckwon@kw.ac.kr

    2014-08-18

    Heavy water (D{sub 2}O) was introduced into a non-thermal plasma-jet (NTPJ) device to generate deuterium monoxide (OD) radicals at room temperature. Owing to the similar reactivity and low prevalence of deuterium in nature, OD radicals can be utilized to visualize the OH radical interactions with water and living cells. Escherichia coli in water were treated with OD radicals, and D atom incorporation into cells was visualized using time-of-flight SIMS and Nano-SIMS. The results show that D atoms from NTPJ reach the cytoplasm of E. coli in H{sub 2}O, indicating the usefulness of this OD-tracking method for the study of radical interactions with living cells.

  1. Rocky Flats Plant Live-Fire Range Risk Analysis Report

    SciTech Connect (OSTI)

    Nicolosi, S.L.; Rodriguez, M.A.

    1994-04-01

    The objective of the Live-Fire Range Risk Analysis Report (RAR) is to provide an authorization basis for operation as required by DOE 5480.16. The existing Live-Fire Range does not have a safety analysis-related authorization basis. EG&G Rocky Flats, Inc. has worked with DOE and its representatives to develop a format and content description for development of an RAR for the Live-Fire Range. Development of the RAR is closely aligned with development of the design for a baffle system to control risks from errant projectiles. DOE 5480.16 requires either an RAR or a safety analysis report (SAR) for live-fire ranges. An RAR rather than a SAR was selected in order to gain flexibility to more closely address the safety analysis and conduct of operation needs for a live-fire range in a cost-effective manner.

  2. Virtual Institute of Microbial Stress and Survival: Deduction of Stress Response Pathways in Metal and Radionuclide Reducing Microorganisms

    SciTech Connect (OSTI)

    2004-04-17

    The projects application goals are to: (1) To understand bacterial stress-response to the unique stressors in metal/radionuclide contamination sites; (2) To turn this understanding into a quantitative, data-driven model for exploring policies for natural and biostimulatory bioremediation; (3) To implement proposed policies in the field and compare results to model predictions; and (4) Close the experimental/computation cycle by using discrepancies between models and predictions to drive new measurements and construction of new models. The projects science goals are to: (1) Compare physiological and molecular response of three target microorganisms to environmental perturbation; (2) Deduce the underlying regulatory pathways that control these responses through analysis of phenotype, functional genomic, and molecular interaction data; (3) Use differences in the cellular responses among the target organisms to understand niche specific adaptations of the stress and metal reduction pathways; (4) From this analysis derive an understanding of the mechanisms of pathway evolution in the environment; and (5) Ultimately, derive dynamical models for the control of these pathways to predict how natural stimulation can optimize growth and metal reduction efficiency at field sites.

  3. Using Rhodobacter Bacteria to Express Membrane Proteins (ANL-IN-99-089) -

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Energy Innovation Portal Biomass and Biofuels Biomass and Biofuels Find More Like This Return to Search Using Rhodobacter Bacteria to Express Membrane Proteins (ANL-IN-99-089) Argonne National Laboratory Contact ANL About This Technology <p> A strategy to express heterologous membrane proteins by using photosynthetic bacteria</p> A strategy to express heterologous membrane proteins by using photosynthetic bacteria Technology Marketing Summary Cell membranes serve as the

  4. Process for generation of hydrogen gas from various feedstocks using thermophilic bacteria

    DOE Patents [OSTI]

    Ooteghem, Suellen Van

    2005-09-13

    A method for producing hydrogen gas is provided comprising selecting a bacteria from the Order Thermotogales, subjecting the bacteria to a feedstock and to a suitable growth environment having an oxygen concentration below the oxygen concentration of water in equilibrium with air; and maintaining the environment at a predetermined pH and at a temperature of at least approximately 45.degree. C. for a time sufficient to allow the bacteria to metabolize the feedstock.

  5. Establishment of stable synthetic mutualism without co-evolution between microalgae and bacteria demonstrated by mutual transfer of metabolites (NanoSIMS isotopic imaging) and persistent physical association (Fluorescent in situ hybridization)

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    de-Bashan, Luz E.; Mayali, Xavier; Bebout, Brad M.; Weber, Peter K.; Detweiler, Angela M.; Hernandez, Juan- Pablo; Prufert-Bebout, Leslie; Bashan, Yoav

    2016-03-03

    The demonstration of a mutualistic interaction requires evidence of benefits for both partners as well as stability of the association over multiple generations. A synthetic mutualism between the freshwater microalga Chlorella sorokiniana and the soil-derived plant growth-promoting bacterium (PGPB) Azospirillum brasilense was created when both microorganisms were co-immobilized in alginate beads. Using stable isotope enrichment experiments followed by high-resolution secondary ion mass spectrometry (SIMS) imaging of single cells, we demonstrated transfer of carbon and nitrogen compounds between the two partners. Further, using fluorescent in situ hybridization (FISH), mechanical disruption and scanning electron microscopy, we demonstrated the stability of their physicalmore » association for a period of 10 days after the aggregated cells were released from the beads. The bacteria significantly enhanced the growth of the microalgae while the microalgae supported growth of the bacteria in a medium where it could not otherwise grow. In conclusion, we propose that this microalga-bacterium association is a true synthetic mutualism independent of co-evolution. (155 words).« less

  6. Selection against spurious promoter motifs correlates withtranslational efficiency across bacteria

    SciTech Connect (OSTI)

    Froula, Jeffrey L.; Francino, M. Pilar

    2007-05-01

    Because binding of RNAP to misplaced sites could compromise the efficiency of transcription, natural selection for the optimization of gene expression should regulate the distribution of DNA motifs capable of RNAP-binding across the genome. Here we analyze the distribution of the -10 promoter motifs that bind the {sigma}{sup 70} subunit of RNAP in 42 bacterial genomes. We show that selection on these motifs operates across the genome, maintaining an over-representation of -10 motifs in regulatory sequences while eliminating them from the nonfunctional and, in most cases, from the protein coding regions. In some genomes, however, -10 sites are over-represented in the coding sequences; these sites could induce pauses effecting regulatory roles throughout the length of a transcriptional unit. For nonfunctional sequences, the extent of motif under-representation varies across genomes in a manner that broadly correlates with the number of tRNA genes, a good indicator of translational speed and growth rate. This suggests that minimizing the time invested in gene transcription is an important selective pressure against spurious binding. However, selection against spurious binding is detectable in the reduced genomes of host-restricted bacteria that grow at slow rates, indicating that components of efficiency other than speed may also be important. Minimizing the number of RNAP molecules per cell required for transcription, and the corresponding energetic expense, may be most relevant in slow growers. These results indicate that genome-level properties affecting the efficiency of transcription and translation can respond in an integrated manner to optimize gene expression. The detection of selection against promoter motifs in nonfunctional regions also implies that no sequence may evolve free of selective constraints, at least in the relatively small and unstructured genomes of bacteria.

  7. Radiation-resistant microorganism

    DOE Patents [OSTI]

    Fliermans, Carl B.

    2007-01-09

    An isolated and purified bacterium is provided which was isolated from a high-level radioactive waste site of mixed waste. The isolate has the ability to degrade a wide variety of organic contaminants while demonstrating high tolerance to ionizing radiation. The organism is uniquely suited to bioremediation of a variety or organic contaminants while in the presence of ionizing radiation.

  8. Radiation-resistant microorganism

    DOE Patents [OSTI]

    Fliermans, Carl B.

    2010-06-15

    An isolated and purified bacterium is provided which was isolated from a high-level radioactive waste site of mixed waste. The isolate has the ability to degrade a wide variety of organic contaminants while demonstrating high tolerance to ionizing radiation. The organism is uniquely suited to bioremediation of a variety or organic contaminants while in the presence of ionizing radiation.

  9. Butanol tolerance in microorganisms

    DOE Patents [OSTI]

    Bramucci, Michael G.; Nagarajan, Vasantha

    2016-03-01

    Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products from a pyruvate utilizing pathway. Yeast host cells provided herein comprise reduced pyruvate decarboxylase activity and modified adenylate cyclase activity. In embodiments, yeast host cells provided herein comprise resistance to butanol and increased biomass production.

  10. Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

    DOE Patents [OSTI]

    Bavykin, Sergei G.; Mirzabekov, Andrei D.

    2007-10-30

    The present invention is directed to a novel method of discriminating a highly infectious bacterium Bacillus anthracis from a group of closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations. The identification and analysis of these sequence variations enables positive discrimination of isolates of the B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed probes, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

  11. Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

    DOE Patents [OSTI]

    Bavykin, Sergei G.; Mirzabekova, legal representative, Natalia V.; Mirzabekov, deceased, Andrei D.

    2007-12-04

    The present invention relates to methods and compositions for using nucleotide sequence variations of 16S and 23S rRNA within the B. cereus group to discriminate a highly infectious bacterium B. anthracis from closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations and discriminate B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed samples, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

  12. Final technical report for project titled Quantitative Characterization of Cell Aggregation/Adhesion as Predictor for Distribution and Transport of Microorganisms in Subsurface Environment

    SciTech Connect (OSTI)

    Gu, April Z; Wan, Kai-tak

    2014-09-02

    This project aims to explore and develop enabling methodology and techniques for nano-scale characterization of microbe cell surface contact mechanics, interactions and adhesion quantities that allow for identification and quantification of indicative properties related to microorganism migration and transport behavior in porous media and in subsurface environments. Microbe transport has wide impact and therefore is of great interest in various environmental applications such as in situ or enhanced subsurface bioremediation,filtration processes for water and wastewater treatments and protection of drinking water supplies. Although great progress has been made towards understanding the identities and activities of these microorganisms in the subsurface, to date, little is known of the mechanisms that govern the mobility and transport of microorganisms in DOE’s contaminated sites, making the outcomes of in situ natural attenuation or contaminant stability enhancement unpredictable. Conventionally, movement of microorganisms was believed to follows the rules governing solute (particle) transport. However, recent studies revealed that cell surface properties, especially those pertaining to cell attachment/adhesion and aggregation behavior, can cause the microbe behavior to deviate from non-viable particles and hence greatly influence the mobility and distribution of microorganisms in porous media.This complexity highlights the need to obtain detailed information of cell-cell and cell-surface interactions in order to improve and refine the conceptual and quantitative model development for fate and transport of microorganisms and contaminant in subsurface. Traditional cell surface characterization methods are not sufficient to fully predict the deposition rates and transport behaviors of microorganism observed. A breakthrough of methodology that would allow for quantitative and molecular-level description of intrinsic cell surface properties indicative for cell

  13. Live Webcast on Recent Wind Energy Technology Advances

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webcast titled “Recent Wind Technology Advances” on April 16, 2014, from 3:00 to 4:00 p.m. Eastern Standard Time.

  14. Senior Adviser Richard Kauffman to Host Live Chat on Renewable...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    WHAT: Senior Adviser to the Secretary of Energy Richard Kauffman to host "Energy Matters" live chat WHEN: Thursday, October 20th 2:00 PM EST HOW: Watch online at Energy.gov To ...

  15. Spontaneous fission half-lives for ground state nuclides

    SciTech Connect (OSTI)

    Holden, N.E. (Brookhaven National Lab., Upton, NY (United States)); Hoffman, D.C. (Lawrence Berkeley Lab., CA (United States))

    1991-01-01

    Measurements of the spontaneous fission half-lives of nuclides of elements Z = 90 to 107 have been compiled and evaluated. Recommended values are presented. 126 refs., 96 tabs.

  16. Live Webinar on the Funding Opportunity for Water Power Manufacturing

    Office of Energy Efficiency and Renewable Energy (EERE)

    On April 23, 2014 from 11:00 AM - 1:00 PM MDT, the Water Power Program will hold a live webinar to provide information to potential applicants for the Water Power Manufacturing Funding Opportunity...

  17. iiESI.org is live! | OpenEI Community

    Open Energy Info (EERE)

    live OpenEI maintenance March 8-9, 2013 Research topics related to ESI Prospects for Nuclear Power(Davis 2012) A Framework for the Optimization of Integrated Energy Systems(Jain...

  18. Watch a Rare Earth Elements Event Live This Morning | Department...

    Broader source: Energy.gov (indexed) [DOE]

    Ginny Simmons Ginny Simmons Former Managing Editor for Energy.gov, Office of Public Affairs From 9:30am to noon ET today you can tune into a live discussion on "rare earth ...

  19. Acting Biomass Program Manager Dr. Valerie Reed to Host Live...

    Broader source: Energy.gov (indexed) [DOE]

    December 16th, the Energy Department (@energy) will be hosting a live Twitter Q&A on biofuels with Dr. Valerie Reed, Acting Manager of the Biomass Program. Dr. Reed holds a Ph. D....

  20. How Will We Live Forever? | GE Global Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Live Forever? Click to email this to a friend (Opens in new window) Share on Facebook (Opens in new window) Click to share (Opens in new window) Click to share on LinkedIn (Opens ...

  1. EIA - Greenhouse Gas Emissions Overview

    Gasoline and Diesel Fuel Update (EIA)

    A2. Glossary Acid stabilization: A circumstance where the pH of the waste mixture in an animal manure management system is maintained near 7.0, optimal conditions for methane production. Aerobic bacteria: Microorganisms living, active, or occurring only in the presence of oxygen. Aerobic decomposition: The breakdown of a molecule into simpler molecules or atoms by microorganisms under favorable conditions of oxygenation. Aerosols: Airborne particles. Afforestation: Planting of new forests on

  2. Clay enhancement of methane, low molecular weight hydrocarbon and halocarbon conversion by methanotrophic bacteria

    DOE Patents [OSTI]

    Apel, William A.; Dugan, Patrick R.

    1995-01-01

    An apparatus and method for increasing the rate of oxidation of toxic vapors by methanotrophic bacteria. The toxic vapors of interest are methane and trichloroethylene. The apparatus includes a gas phase bioreactor within a closed loop pumping system or a single pass system. The methanotrophic bacteria include Methylomonas methanica, Methylosinus trichosporium, and uncharacterized environmental enrichments.

  3. Clay enhancement of methane, low molecular weight hydrocarbon and halocarbon conversion by methanotrophic bacteria

    DOE Patents [OSTI]

    Apel, William A.; Dugan, Patrick R.

    1995-04-04

    An apparatus and method for increasing the rate of oxidation of toxic vapors by methanotrophic bacteria. The toxic vapors of interest are methane and trichloroethylene. The apparatus includes a gas phase bioreactor within a closed loop pumping system or a single pass system. The methanotrophic bacteria include Methylomonas methanica, Methylosinus trichosporium, and uncharacterized environmental enrichments.

  4. Live from Greenbuild: From the Industrial Facilities Connect & Learn |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Live from Greenbuild: From the Industrial Facilities Connect & Learn Live from Greenbuild: From the Industrial Facilities Connect & Learn November 18, 2015 - 5:32pm Addthis By Monica Kanojia The industrial industry is filled with unique and dynamic projects with substantially high process loads and resource consumption. This sector faces a different set of challenges in attaining sustainably built campuses. Owners must address compliance, regulations and safety

  5. Portsmouth Educational Outreach Seeks to Transform Lives of Area Students |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Educational Outreach Seeks to Transform Lives of Area Students Portsmouth Educational Outreach Seeks to Transform Lives of Area Students January 29, 2014 - 12:00pm Addthis Students participating in the Science Alliance enjoyed hands-on exhibits and discussions with scientists, engineers and specialists in a range of fields. Students participating in the Science Alliance enjoyed hands-on exhibits and discussions with scientists, engineers and specialists in a range of

  6. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Tracking Living Cells as They Differentiate in Real Time Print Thursday, 27 September 2012 00:00 Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible

  7. Invisible Science: Lab Breakthroughs in Our Daily Lives | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Invisible Science: Lab Breakthroughs in Our Daily Lives Invisible Science: Lab Breakthroughs in Our Daily Lives April 24, 2012 - 2:30pm Addthis The Lab Breakthroughs video series focuses on the array of technological advancements and discoveries that stem from research performed in the National Labs, including improvements in industrial processes, discoveries in fundamental scientific research, and innovative medicines. <a href="http://energy.gov/lab-breakthroughs">See

  8. Public invited to share living with wildfire stories with BSM

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Public invited to share living with wildfire stories with BSM Public invited to share living with wildfire stories with BSM The exhibit provides an opportunity for people to share their stories about the Las Conchas fire and other wildfires. June 11, 2012 Personal experiences and stories around wildfire are part of a new interactive exhibit at the Bradbury. Personal experiences and stories around wildfire are part of a new interactive exhibit at the Bradbury. Contact Steve Sandoval

  9. Living Comfortably: A Consumer's Guide to Home Energy Upgrades |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Living Comfortably: A Consumer's Guide to Home Energy Upgrades Living Comfortably: A Consumer's Guide to Home Energy Upgrades March 7, 2013 - 3:15pm Addthis A weatherization worker drills holes to blow cellulose insulation in the interior walls of this home. | Photo courtesy of Dennis Schroeder, NREL A weatherization worker drills holes to blow cellulose insulation in the interior walls of this home. | Photo courtesy of Dennis Schroeder, NREL Dr. Richard Knaub Project

  10. Unearthing the ecology of soil microorganisms using a high resolution DNA-SIP approach to explore cellulose and xylose metabolism in soil

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Pepe-Ranney, Charles; Campbell, Ashley N.; Koechli, Chantal N.; Berthrong, Sean; Buckley, Daniel H.

    2016-05-12

    We explored microbial contributions to decomposition using a sophisticated approach to DNA Stable Isotope Probing (SIP). Our experiment evaluated the dynamics and ecological characteristics of functionally defined microbial groups that metabolize labile and structural C in soils. We added to soil a complex amendment representing plant derived organic matter substituted with either 13C-xylose or 13C-cellulose to represent labile and structural C pools derived from abundant components of plant biomass. We found evidence for 13C-incorporation into DNA from 13C-xylose and 13C-cellulose in 49 and 63 operational taxonomic units (OTUs), respectively. The types of microorganisms that assimilated 13C in the 13C-xylose treatmentmore » changed over time being predominantly Firrnicutes at day 1 followed by Bacteroidetes at day 3 and then Actinobacteria at day 7. These 13C-labeling dynamics suggest labile C traveled through different trophic levels. In contrast, microorganisms generally metabolized cellulose-C after 14 days and did not change to the same extent in phylogenetic composition over time. Furthermore, microorganisms that metabolized cellulose-C belonged to poorly characterized but cosmopolitan soil lineages including Verrucomicrobia, Chlorotlexi, and Planctomycetes.« less

  11. LiveWall Operational Evaluation: Seattle Law Enforcement Pilot

    SciTech Connect (OSTI)

    Barr, Jonathan L.; Burtner, Edwin R.; Stein, Steven L.

    2013-10-01

    The LiveWall concept envisioned as an outgrowth of the Precision Information Environment (PIE) project allows communications between separate groups using interactive video, audio, and a shared desktop environment; this allows everyone to participate and collaborate in real time, regardless of location. The LiveWall concept provides a virtual window to other locations, where all parties can interact and collaboratively work with each other. This functionality is intended to improve multi-site coordination amongst emergency operations centers (EOC), field operations sites and across organizations and jurisdictions to accommodate communications during routine and emergency events. For the initial LiveWall operational evaluation PNNL partnered with the Seattle Police Department (SPD). This partnership allowed for the creation of an excellent LiveWall test bed specific to law enforcement. This partnership made it possible to test the LiveWall concept with scenarios involving the many facets of the law enforcement work done by SPD. PNNL and SPD agreed that integrating the systems into operations for a real event would be the best test of the technology and give SPD staff greater visibility into the functionality and benefits offered by the LiveWall concept.

  12. Production of ethanol from lignocellulosic materials using thermophilic bacteria

    SciTech Connect (OSTI)

    Lynd, L.R.

    1987-01-01

    The production of ethanol from lignocellulosic materials, e.g. wood, agricultural residues, and municipal solid wastes, is considered. The conversion of these materials to ethanol in the US could annually yield approximately 430 million tons ethanol, or about 9.8 quads, within the next 20 years. Thermophilic bacteria have advantages over yeasts for ethanol production because various species produce an active cellulase enzyme and utilize pentose sugars. However thermophiles have lower ethanol tolerance and usually lower ethanol yields. The potential of thermophilic ethanol production from hardwood chips is examined in detail. It is concluded that if high ethanol yield can be achieved this process could have economics competitive with either ethanol production from corn via yeast or synthetic production from ethylene. Low ethanol tolerance is not a major problem provided concentrations {ge} 1.5% are produced, ethanol is continuously removed from the fermentor, and IHOSR/extractive distillation is employed. Research was undertaken aimed at closing the gap between the attractive potential of thermophiles for ethanol production, and that which is possible based on present knowledge, which is not practical. Major topics were the activity of Clostridium thermocellum cellulase on pretreated mixed hardwood and Avicel in vivo, continuous culture of C. thermocellum on pretreated mixed hardwood and Avicel, and the continuous culture of Clostridium thermosaccharolyticum at high xylose concentrations in the presence and absence of ethanol removal.

  13. Night of the Living Trash: Bringing Your Waste Back to Life ...

    Office of Environmental Management (EM)

    Night of the Living Trash: Bringing Your Waste Back to Life Night of the Living Trash: Bringing Your Waste Back to Life October 30, 2015 - 12:33pm Addthis Night of the Living ...

  14. Characterization of microbial associations with methanotrophic archaea and sulfate-reducing bacteria through statistical comparison of nested Magneto-FISH enrichments

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Trembath-Reichert, Elizabeth; Case, David H.; Orphan, Victoria J.

    2016-04-18

    Methane seep systems along continental margins host diverse and dynamic microbial assemblages, sustained in large part through the microbially mediated process of sulfate-coupled Anaerobic Oxidation of Methane (AOM). This methanotrophic metabolism has been linked to consortia of anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing bacteria (SRB). These two groups are the focus of numerous studies; however, less is known about the wide diversity of other seep associated microorganisms. We selected a hierarchical set of FISH probes targeting a range ofDeltaproteobacteriadiversity. Using the Magneto-FISH enrichment technique, we then magnetically captured CARD-FISH hybridized cells and their physically associated microorganisms from a methane seepmore » sediment incubation. DNA from nested Magneto-FISH experiments was analyzed using Illumina tag 16S rRNA gene sequencing (iTag). Enrichment success and potential bias with iTag was evaluated in the context of full-length 16S rRNA gene clone libraries, CARD-FISH, functional gene clone libraries, and iTag mock communities. We determined commonly used Earth Microbiome Project (EMP) iTAG primers introduced bias in some common methane seep microbial taxa that reduced the ability to directly compare OTU relative abundances within a sample, but comparison of relative abundances between samples (in nearly all cases) and whole community-based analyses were robust. The iTag dataset was subjected to statistical co-occurrence measures of the most abundant OTUs to determine which taxa in this dataset were most correlated across all samples. In addition, many non-canonical microbial partnerships were statistically significant in our co-occurrence network analysis, most of which were not recovered with conventional clone library sequencing, demonstrating the utility of combining Magneto-FISH and iTag sequencing methods for hypothesis generation of associations within complex microbial communities. Network analysis pointed

  15. Estimating Bacteria Emissions from Inversion of Atmospheric Transport: Sensitivity to Modelled Particle Characteristics

    SciTech Connect (OSTI)

    Burrows, Susannah M.; Rayner, Perter; Butler, T.; Lawrence, M.

    2013-06-04

    Model-simulated transport of atmospheric trace components can be combined with observed concentrations to obtain estimates of ground-based sources using various inversion techniques. These approaches have been applied in the past primarily to obtain source estimates for long-lived trace gases such as CO2. We consider the application of similar techniques to source estimation for atmospheric aerosols, by using as a case study the estimation of bacteria emissions from different ecosystem regions in the global atmospheric chemistry and climate model ECHAM5/MESSy-Atmospheric Chemistry (EMAC). Simulated particle concentrations in the tropopause region and at high latitudes, as well as transport of particles to tundra and land ice regions are shown to be highly sensitive to scavenging in mixed-phase clouds, which is poorly characterized in most global climate models. This may be a critical uncertainty in correctly simulating the transport of aerosol particles to the Arctic. Source estimation via Monte Carlo Markov Chain is applied to a suite of sensitivity simulations and the global mean emissions are estimated. We present an analysis of the partitioning of uncertainties in the global mean emissions that are attributable to particle size, CCN activity, the ice nucleation scavenging ratios for mixed-phase and cold clouds, and measurement error. Uncertainty due to CCN activity or to a 1 um error in particle size is typically between 10% and 40% of the uncertainty due to data uncertainty, as measured by the 5%-ile to 95%-ile range of the Monte Carlo ensemble. Uncertainty attributable to the ice nucleation scavenging ratio in mized-phase clouds is as high as 10% to 20% of the data uncertainty. Taken together, the four model 20 parameters examined contribute about half as much to the uncertainty in the estimated emissions as do the measurements. This was a surprisingly large contribution from model uncertainty in light of the substantial data uncertainty, which ranges from 81

  16. TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing...

    Energy Savers [EERE]

    Live Chat on Reducing America's Oil Dependence Through Innovation TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's Oil Dependence Through Innovation ...

  17. Newly identified helper bacteria stimulate ectomycorrhizal formation in Populus

    SciTech Connect (OSTI)

    Labbe, Jessy L.; Weston, David J.; Dunkirk, Nora; Pelletier, Dale A.; Tuskan, Gerald A.

    2014-10-24

    Mycorrhiza helper bacteria (MHB) are known to increase host root colonization by mycorrhizal fungi but the molecular mechanisms and potential tripartite trophic interactions are poorly understood. Through an effort to study Populus microbiome, we isolated 21 Pseudomonas strains from native Populus deltoides roots. These bacterial isolates were characterized and screened for MHB effectiveness on the Populus-Laccaria system. Two other Pseudomonas strains (i.e., Pf-5 and BBc6R8) from existing collections were also included as reference in the screening process. We analyzed Laccaria bicolor S238N growth rate, mycelial architecture and transcriptional changes induced by the contrasting Pseudomonas strains (i.e., inhibitory, neutral and beneficial). We characterized 17 out of the 21 Pseudomonas strains from the Populus rhizosphere with positive effects on L. bicolor S238N growth, as well as on Populus root architecture and colonization by L. bicolor S238N across three Populus species. Four of seven reporter genes, Tra1, Tectonin2, Gcn5 and Cipc1, thought to be specific to the interaction with strain BBc6R8, were induced or repressed while interacting with six (i.e., GM17, GM33, GM41, GM48, Pf-5 and BBc6R8) of the tested Pseudomonas strains. GM41 promoted the highest roots colonization across three Populus species but most notably in P. deltoides, which is otherwise, poorly colonized by L. bicolor. Here we report novel MHB strains isolated from native Populus that improve roots colonization. This tripartite relationship could be exploited in nursery production for target Populus species/genotypes as a means of improving establishment and survival in marginal lands.

  18. Newly identified helper bacteria stimulate ectomycorrhizal formation in Populus

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Labbe, Jessy L.; Weston, David J.; Dunkirk, Nora; Pelletier, Dale A.; Tuskan, Gerald A.

    2014-10-24

    Mycorrhiza helper bacteria (MHB) are known to increase host root colonization by mycorrhizal fungi but the molecular mechanisms and potential tripartite trophic interactions are poorly understood. Through an effort to study Populus microbiome, we isolated 21 Pseudomonas strains from native Populus deltoides roots. These bacterial isolates were characterized and screened for MHB effectiveness on the Populus-Laccaria system. Two other Pseudomonas strains (i.e., Pf-5 and BBc6R8) from existing collections were also included as reference in the screening process. We analyzed Laccaria bicolor S238N growth rate, mycelial architecture and transcriptional changes induced by the contrasting Pseudomonas strains (i.e., inhibitory, neutral and beneficial).more » We characterized 17 out of the 21 Pseudomonas strains from the Populus rhizosphere with positive effects on L. bicolor S238N growth, as well as on Populus root architecture and colonization by L. bicolor S238N across three Populus species. Four of seven reporter genes, Tra1, Tectonin2, Gcn5 and Cipc1, thought to be specific to the interaction with strain BBc6R8, were induced or repressed while interacting with six (i.e., GM17, GM33, GM41, GM48, Pf-5 and BBc6R8) of the tested Pseudomonas strains. GM41 promoted the highest roots colonization across three Populus species but most notably in P. deltoides, which is otherwise, poorly colonized by L. bicolor. Here we report novel MHB strains isolated from native Populus that improve roots colonization. This tripartite relationship could be exploited in nursery production for target Populus species/genotypes as a means of improving establishment and survival in marginal lands.« less

  19. Search for Charged Massive Long-Lived Particles

    SciTech Connect (OSTI)

    Abazov V. M.; Abbott B.; Acharya B. S.; Adams M.; Adams T.; Alexeev G. D.; Alimena J.; Alkhazov G.; Alton A.; Alverson G.; Alves G. A.; Aoki M.; Askew A.; Asman B.; Atkins S.; Atramentov O.; Augsten K.; Avila C.; BackusMayes J.; Badaud F.; Bagby L.; Baldin B.; Bandurin D. V.; Banerjee S.; Barberis E.; Baringer P.; Barreto J.; Bartlett J. F.; Bassler U.; Bazterra V.; Bean A.; Begalli M.; Belanger-Champagne C.; Bellantoni L.; Beri S. B.; Bernardi G.; Bernhard R.; Bertram I.; Besancon M.; Beuselinck R.; Bezzubov V. A.; Bhat P. C.; Bhatnagar V.; Blazey G.; Blessing S.; Bloom K.; Boehnlein A.; Boline D.; Boos E. E.; Borissov G.; Bose T.; Brandt A.; Brandt O.; Brock R.; Brooijmans G.; Bross A.; Brown D.; Brown J.; Bu X. B.; Buehler M.; Buescher V.; Bunichev V.; Burdin S.; Burnett T. H.; Buszello C. P.; Calpas B.; Camacho-Perez E.; Carrasco-Lizarraga M. A.; Casey B. C. K.; Castilla-Valdez H.; Chakrabarti S.; Chakraborty D.; Chan K. M.; Chandra A.; Chapon E.; Chen G.; Chevalier-Thery S.; Cho D. K.; Cho S. W.; Choi S.; Choudhary B.; Cihangir S.; Claes D.; Clutter J.; Cooke M.; Cooper W. E.; Corcoran M.; Couderc F.; Cousinou M. -C.; Croc A.; Cutts D.; Das A.; Davies G.; De K.; de Jong S. J.; De la Cruz-Burelo E.; Deliot F.; Demina R.; Denisov D.; Denisov S. P.; Desai S.; Deterre C.; DeVaughan K.; Diehl H. T.; Diesburg M.; Ding P. F.; Dominguez A.; Dorland T.; Dubey A.; Dudko L. V.; Duggan D.; Duperrin A.; Dutt S.; Dyshkant A.; Eads M.; Edmunds D.; Ellison J.; Elvira V. D.; Enari Y.; Evans H.; Evdokimov A.; Evdokimov V. N.; Facini G.; Ferbel T.; Fiedler F.; Filthaut F.; Fisher W.; Fisk H. E.; Fortner M.; Fox H.; Fuess S.; Garcia-Bellido A.; Garcia-Guerra G. A.; Gavrilov V.; Gay P.; Geng W.; Gerbaudo D.; Gerber C. E.; Gershtein Y.; Ginther G.; Golovanov G.; Goussiou A.; Grannis P. D.; Greder S.; Greenlee H.; Greenwood Z. D.; Gregores E. M.; Grenier G.; Gris Ph.; Grivaz J. -F.; Grohsjean A.; Gruenendahl S.; Gruenewald M. W.; Guillemin T.; Gutierrez G.; Gutierrez P.; Haas A.; Hagopian S.; Haley J.; Han L.; Harder K.; Harel A.; Hauptman J. M.; Hays J.; Head T.; Hebbeker T.; Hedin D.; Hegab H.; Heinson A. P.; Heintz U.; Hensel C.; Heredia-De La Cruz I.; Herner K.; Hesketh G.; Hildreth M. D.; Hirosky R.; Hoang T.; Hobbs J. D.; Hoeneisen B.; Hohlfeld M.; Hubacek Z.; Hynek V.; Iashvili I.; Ilchenko Y.; Illingworth R.; Ito A. S.; Jabeen S.; Jaffre M.; Jamin D.; Jayasinghe A.; Jesik R.; Johns K.; Johnson M.; Jonckheere A.; Jonsson P.; Joshi J.; Jung A. W.; Juste A.; Kaadze K.; Kajfasz E.; Karmanov D.; Kasper P. A.; Katsanos I.; Kehoe R.; Kermiche S.; Khalatyan N.; Khanov A.; Kharchilava A.; Kharzheev Y. N.; Kohli J. M.; Kozelov A. V.; Kraus J.; Kulikov S.; Kumar A.; Kupco A.; Kurca T.; Kuzmin V. A.; Kvita J.; Lammers S.; Landsberg G.; Lebrun P.; Lee H. S.; Lee S. W.; Lee W. M.; Lellouch J.; Li L.; Li Q. Z.; Lietti S. M.; Lim J. K.; Lincoln D.; Linnemann J.; Lipaev V. V.; Lipton R.; Liu Y.; Lobodenko A.; Lokajicek M.; de Sa R. Lopes; Lubatti H. J.; Luna-Garcia R.; Lyon A. L.; Maciel A. K. A.; Mackin D.; Madar R.; Magana-Villalba R.; Malik S.; Malyshev V. L.; Maravin Y.; Martinez-Ortega J.; McCarthy R.; McGivern C. L.; Meijer M. M.; et al.

    2012-03-21

    We report on a search for charged massive long-lived particles (CMLLPs), based on 5.2 fb{sup -1} of integrated luminosity collected with the D0 detector at the Fermilab Tevatron p{bar p} collider. We search for events in which one or more particles are reconstructed as muons but have speed and ionization energy loss (dE/dx) inconsistent with muons produced in beam collisions. CMLLPs are predicted in several theories of physics beyond the standard model. We exclude pair-produced long-lived gaugino-like charginos below 267 GeV and Higgsino-like charginos below 217 GeV at 95% C.L., as well as long-lived scalar top quarks with mass below 285 GeV.

  20. Living well, doing good | Y-12 National Security Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Living well, doing good Living well, doing good Posted: June 24, 2015 - 3:22pm Joe Marshall is one of Y-12's cancer survivors who took his victory lap at the Anderson County Relay for Life. What do relays, races and regattas have in common? They're all ways Y-12 employees stay active and support the community. After hours, Y-12ers are all about staying active and supporting their communities. Here's a roundup of the activities Relay for Life, May 16 For numerous years, Joe Kato and his

  1. Interested in Open Energy Data? Watch the Energy Datapalooza Live! |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Interested in Open Energy Data? Watch the Energy Datapalooza Live! Interested in Open Energy Data? Watch the Energy Datapalooza Live! September 27, 2012 - 5:25pm Addthis U.S. Chief Technology Officer Todd Park kicks off the Safety Datapalooza on September 19th. | Photo Courtesy U.S. Department of Labor U.S. Chief Technology Officer Todd Park kicks off the Safety Datapalooza on September 19th. | Photo Courtesy U.S. Department of Labor Matthew Loveless Matthew Loveless

  2. Lab Breakthrough: How Energy Department Research Saves Lives | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy How Energy Department Research Saves Lives Lab Breakthrough: How Energy Department Research Saves Lives August 28, 2013 - 12:50pm Addthis Researchers at the National Energy Technology Lab have developed a platinum-chromium alloy that is used to make heart stents that are thin, flexible, corrosion resistant and visible on x-rays. Since their introduction in 2010, coronary stents made from the new alloy have generated more than $4 billion in worldwide sales and captured 45 percent of

  3. Advanced Biofuels: How Scientists are Engineering Bacteria to Help Drive America

    Broader source: Energy.gov [DOE]

    Researchers at the Energy Department's Joint BioEnergy Institute (JBEI) have engineered the first strains of the bacteria to digest switchgrass biomass and synthesize its sugars into all three types of transportation fuels -- gasoline, diesel and jet fuels.

  4. Amoebae/bacteria consortia and uses for degrading wastes and contaminants

    DOE Patents [OSTI]

    Tyndall, Richard L.

    1996-01-01

    A method of altering trinitrotoluene includes the steps of: providing an amoeba/bacteria consortium, particularly ATCC 40908 or a mutant thereof possessing all the identifying characteristics thereof; and contacting the consortium with trinitrotoluene to alter the trinitrotoluene.

  5. Geek-Up[09.24.10] -- Magical BEANs, Combating Bacteria's Resistance...

    Energy Savers [EERE]

    and crystallized the membrane proteins that make up an E. coli bacteria's efflux pump. These pumps, which are comprised of crystal structures in the membrane, remove heavy-metal ...

  6. Real time imaging of live cell ATP leaking or release events by chemiluminescence microscopy

    SciTech Connect (OSTI)

    Zhang, Yun

    2008-12-18

    The purpose of this research was to expand the chemiluminescence microscopy applications in live bacterial/mammalian cell imaging and to improve the detection sensitivity for ATP leaking or release events. We first demonstrated that chemiluminescence (CL) imaging can be used to interrogate single bacterial cells. While using a luminometer allows detecting ATP from cell lysate extracted from at least 10 bacterial cells, all previous cell CL detection never reached this sensitivity of single bacteria level. We approached this goal with a different strategy from before: instead of breaking bacterial cell membrane and trying to capture the transiently diluted ATP with the firefly luciferase CL assay, we introduced the firefly luciferase enzyme into bacteria using the modern genetic techniques and placed the CL reaction substrate D-luciferin outside the cells. By damaging the cell membrane with various antibacterial drugs including antibiotics such as Penicillins and bacteriophages, the D-luciferin molecules diffused inside the cell and initiated the reaction that produces CL light. As firefly luciferases are large protein molecules which are retained within the cells before the total rupture and intracellular ATP concentration is high at the millmolar level, the CL reaction of firefly luciferase, ATP and D-luciferin can be kept for a relatively long time within the cells acting as a reaction container to generate enough photons for detection by the extremely sensitive intensified charge coupled device (ICCD) camera. The result was inspiring as various single bacterium lysis and leakage events were monitored with 10-s temporal resolution movies. We also found a new way of enhancing diffusion D-luciferin into cells by dehydrating the bacteria. Then we started with this novel single bacterial CL imaging technique, and applied it for quantifying gene expression levels from individual bacterial cells. Previous published result in single cell gene expression quantification

  7. Purple Bacteria Develops Its Own Form of Sunscreen | U.S. DOE Office of

    Office of Science (SC) Website

    Science (SC) Purple Bacteria Develops Its Own Form of "Sunscreen" Energy Frontier Research Centers (EFRCs) EFRCs Home Centers Research Science Highlights Highlight Archives News & Events Publications History Contact BES Home 05.03.12 Purple Bacteria Develops Its Own Form of "Sunscreen" Print Text Size: A A A FeedbackShare Page Scientific Achievement Found that specific pigments in the light harvesting complex of a photosynthetic bacterium act primarily to protect the

  8. Have You Visited the Living Zero Home Tour?

    Broader source: Energy.gov [DOE]

    The Living Zero Home Tour is on the road, and it may be in a city near you! If you have the chance to see the tour, you may be surprised to discover the small steps that you can take to save energy...

  9. Lead in human blood from children living in Campania, Italy

    SciTech Connect (OSTI)

    Amodio-Cocchieri, R.; Arnese, A.; Prospero, E.; Roncioni, A.

    1996-03-01

    Blood lead (PbB) levels were determined in children living Campania (in Naples and in a rural zone in the district of Caserta). Atmospheric lead (PbA) concentration in these considered areas was monitored for 1 yr (1993-1994). The children tested were questioned about common sources of lead, other than atmospheric relating to their living and dietary habits. The PbB levels in children living in Naples were at the 50th percentile, 18.8 {mu}g/dl in males and 13.7 {mu}g/dl in females; in children living in the rural area the median PbB levels were 8.9{mu}g/dl in males, and 9.9 {mu}g/dl in females. The annual mean values of atmospheres lead were 1.15 {plus_minus} 0.24 {mu}g/dl in Naples and 0.23 {plus_minus} 0.07 {mu}g/dl in the rural area. Significant and congruent mean differences between urban and rural sites were found in children`s blood and concurrent air lead. Considering the PbB level of 10 {mu}g/dl as the maximum level that is not associated any known adverse effect in children, the Neapolitan group can be considered at risk of chronic intoxication by lead. 18 refs., 3 figs., 4 tabs.

  10. Connecticut Weatherization Project Improves Lives, Receives National Recognition

    Broader source: Energy.gov [DOE]

    Several energy-efficient improvements made to a senior care center in New Milford, Connecticut are helping residents live healthier and more comfortable lifestyles. The upgrade to the facility also captured a residential energy efficiency award and is an example for other states.

  11. Understanding dynamic changes in live cell adhesion with neutron reflectometry

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Junghans, Ann; Waltman, Mary Jo; Smith, Hillary L.; Pocivavsek, Luka; Zebda, Noureddine; Birukov, Konstantin; Viapiano, Mariano; Majewski, Jaroslaw

    2014-12-10

    In this study, neutron reflectometry (NR) was used to examine various live cells' adhesion to quartz substrates under different environmental conditions, including flow stress. To the best of our knowledge, these measurements represent the first successful visualization and quantization of the interface between live cells and a substrate with sub-nanometer resolution. In our first experiments, we examined live mouse fibroblast cells as opposed to past experiments using supported lipids, proteins, or peptide layers with no associated cells. We continued the NR studies of cell adhesion by investigating endothelial monolayers and glioblastoma cells under dynamic flow conditions. We demonstrated that neutronmore » reflectometry is a powerful tool to study the strength of cellular layer adhesion in living tissues, which is a key factor in understanding the physiology of cell interactions and conditions leading to abnormal or disease circumstances. Continuative measurements, such as investigating changes in tumor cell — surface contact of various glioblastomas, could impact advancements in tumor treatments. In principle, this can help us to identify changes that correlate with tumor invasiveness. Pursuit of these studies can have significant medical impact on the understanding of complex biological problems and their effective treatment, e.g. for the development of targeted anti-invasive therapies.« less

  12. Understanding dynamic changes in live cell adhesion with neutron reflectometry

    SciTech Connect (OSTI)

    Junghans, Ann; Waltman, Mary Jo; Smith, Hillary L.; Pocivavsek, Luka; Zebda, Noureddine; Birukov, Konstantin; Viapiano, Mariano; Majewski, Jaroslaw

    2014-12-10

    In this study, neutron reflectometry (NR) was used to examine various live cells' adhesion to quartz substrates under different environmental conditions, including flow stress. To the best of our knowledge, these measurements represent the first successful visualization and quantization of the interface between live cells and a substrate with sub-nanometer resolution. In our first experiments, we examined live mouse fibroblast cells as opposed to past experiments using supported lipids, proteins, or peptide layers with no associated cells. We continued the NR studies of cell adhesion by investigating endothelial monolayers and glioblastoma cells under dynamic flow conditions. We demonstrated that neutron reflectometry is a powerful tool to study the strength of cellular layer adhesion in living tissues, which is a key factor in understanding the physiology of cell interactions and conditions leading to abnormal or disease circumstances. Continuative measurements, such as investigating changes in tumor cell — surface contact of various glioblastomas, could impact advancements in tumor treatments. In principle, this can help us to identify changes that correlate with tumor invasiveness. Pursuit of these studies can have significant medical impact on the understanding of complex biological problems and their effective treatment, e.g. for the development of targeted anti-invasive therapies.

  13. Genome sequence of the Fleming strain of Micrococcus luteus, a simple free- living actinobacterium

    SciTech Connect (OSTI)

    Young, Michael; Artsatbanov, Vladislav; Beller, Harry R.; Chandra, Govind; Chater, Keith F.; Dover, Lynn G.; Goh, Ee-Been; Kahan, Tamar; Kaprelyants, Arseny S.; Kyrpides, Nikos; Lapidus, Alla; Lowry, Stephen R.; Lykidis, Athanasios; Mahillon, Jacques; Markowitz, Viktor; Mavrommatis, Konstantinos; Mukamolova, Galina V.; Oren, Aharon; Rokem, J. Stefan; Smith, Margaret C. M.; Young, Danielle I.; Greenblatt, Charles L.

    2009-11-01

    Micrococcus luteus (NCTC2665, Fleming strain) has one of the smallest genomes of free living actinobacteria sequenced to date, comprising a single circular chromosome of 2,501,097 bp (G+C content 73%) predicted to encode 2403 proteins. The genome shows extensive synteny with that of the closely related organism, Kocuria rhizophila, from which it was taxonomically separated relatively recently. Despite its small size, the genome harbors 73 IS elements, almost all of which are closely related to elements found in other actinobacteria. An IS element is inserted into the rrs gene of one of only two rrn operons found in M. luteus. The genome encodes only four sigma factors and fourteen response regulators, indicative of adaptation to a rather strict ecological niche (mammalian skin). The high sensitivity of M. luteus to {Beta}-lactam antibiotics may result from the presence of a reduced set of penicillin binding proteins and the absence of a wblC gene, which plays an important role in antibiotic resistance in other actinobacteria. Consistent with the restricted range of compounds it can use as a sole source of carbon for energy and growth, M. luteus has a minimal complement of genes concerned with carbohydrate transport and metabolism and its inability to utilize glucose as a sole carbon source may be due to the apparent absence of a gene encoding glucokinase. Uniquely among characterized bacteria, M. luteus appears to be able to metabolize glycogen only via trehalose, and to make trehalose only via glycogen. It has very few genes associated with secondary metabolism. In contrast to other actinobacteria, M. luteus encodes only one resuscitation-promoting factor (Rpf) required for emergence from dormancy and its complement of other dormancy-related proteins is also much reduced. M. luteus is capable of long-chain alkene biosynthesis, which is of interest for advanced biofuel production; a three gene cluster essential for this metabolism has been identified in the genome.

  14. The problem of living in a world contaminated with chemicals

    SciTech Connect (OSTI)

    Metcalf, R.L.

    1990-12-31

    The proliferation of xenobiotic chemicals in the global environment poses living problems for each of us aboard {open_quotes}spaceship earth.{close_quotes} Seven case studies are presented that illustrate the magnitude of the problem that can result from waiting to identify toxic hazards until there have been decades of {open_quotes}human guinea pig{close_quotes} exposure. 25 refs., 5 tabs.

  15. 'Zombie' Replica Cells May Outperform Live Ones as Catalysts and

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Conductors 'Zombie' Replica Cells May Outperform Live Ones as Catalysts and Conductors - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery

  16. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible to watch phosphorylation at the molecular level without damaging cells or interfering with

  17. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible to watch phosphorylation at the molecular level without damaging cells or interfering with

  18. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible to watch phosphorylation at the molecular level without damaging cells or interfering with

  19. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible to watch phosphorylation at the molecular level without damaging cells or interfering with

  20. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible to watch phosphorylation at the molecular level without damaging cells or interfering with

  1. Single-photon ultrashort-lived radionuclides: symposium proceedings

    SciTech Connect (OSTI)

    Paras, P.; Thiessen, J.W. (eds.)

    1985-01-01

    The purpose was to define the current role and state-of-the-art regarding the development, clinical applications, and usefulness of generator-produced single-photon ultrashort-lived radionuclides (SPUSLR's) and to predict their future impact on medicine. Special emphasis was placed on the generator production of iridium-191, gold-195, and krypton-81. This report contains expanded summaries of the included papers. (ACR)

  2. ClimateChangeLIVE Webcast: Join the Climate Conversation

    Broader source: Energy.gov [DOE]

    Join ClimateChangeLIVE's webcast, bringing together students and climate experts for a discussion about climate change and what students and classes around the country are doing to be part of the climate solution. Students will be able to interact with climate scientists and experts online through Facebook and Twitter. A GreenWorks! grant will be offered to help schools with climate action projects.

  3. Liquid Fuel From Renewable Electricity and Bacteria: Electro-Autotrophic Synthesis of Higher Alcohols

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: UCLA is utilizing renewable electricity to power direct liquid fuel production in genetically engineered Ralstonia eutropha bacteria. UCLA is using renewable electricity to convert carbon dioxide into formic acid, a liquid soluble compound that delivers both carbon and energy to the bacteria. The bacteriaare genetically engineered to convert the formic acid into liquid fuelin this case alcohols such as butanol. The electricity required for the process can be generated from sunlight, wind, or other renewable energy sources. In fact, UCLAs electricity-to-fuel system could be a more efficient way to utilize these renewable energy sources considering the energy density of liquid fuel is much higher than the energy density of other renewable energy storage options, such as batteries.

  4. Genetically Modified Bacteria for Fuel Production: Development of Rhodobacteria as a Versatile Platform for Fuels Production

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: Penn State is genetically engineering bacteria called Rhodobacter to use electricity or electrically generated hydrogen to convert carbon dioxide into liquid fuels. Penn State is taking genes from oil-producing algae called Botryococcus braunii and putting them into Rhodobacter to produce hydrocarbon molecules, which closely resemble gasoline. Penn State is developing engineered tanks to support microbial fuel production and determining the most economical way to feed the electricity or hydrogen to the bacteria, including using renewable sources of power like solar energy.

  5. Geek-Up[09.24.10] -- Magical BEANs, Combating Bacteria's Resistance to

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Antibiotics and the ChemCam's Journey to Mars | Department of Energy 9.24.10] -- Magical BEANs, Combating Bacteria's Resistance to Antibiotics and the ChemCam's Journey to Mars Geek-Up[09.24.10] -- Magical BEANs, Combating Bacteria's Resistance to Antibiotics and the ChemCam's Journey to Mars September 24, 2010 - 5:19pm Addthis Check out the ChemCam close-up, which will reveal which elements are present in Mars' rocks and soils. Elizabeth Meckes Elizabeth Meckes Director of User Experience

  6. Geek-Up[12.03.2010]: Halomonadaceae Bacteria and the Return of Quark Gluon

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Plasma | Department of Energy 2.03.2010]: Halomonadaceae Bacteria and the Return of Quark Gluon Plasma Geek-Up[12.03.2010]: Halomonadaceae Bacteria and the Return of Quark Gluon Plasma December 3, 2010 - 4:59pm Addthis Niketa Kumar Niketa Kumar Public Affairs Specialist, Office of Public Affairs As a fellow geek, you probably heard NASA's big news yesterday. If not, here's a recap: It was once assumed that all life on Earth, from humans and giraffes to scorpions and plankton, are all based

  7. Non-destructive observation of intact bacteria and viruses in water by the highly sensitive frequency transmission electric-field method based on SEM

    SciTech Connect (OSTI)

    Ogura, Toshihiko

    2014-08-08

    Highlights: We developed a high-sensitive frequency transmission electric-field (FTE) system. The output signal was highly enhanced by applying voltage to a metal layer on SiN. The spatial resolution of new FTE method is 41 nm. New FTE system enables observation of the intact bacteria and virus in water. - Abstract: The high-resolution structural analysis of biological specimens by scanning electron microscopy (SEM) presents several advantages. Until now, wet bacterial specimens have been examined using atmospheric sample holders. However, images of unstained specimens in water using these holders exhibit very poor contrast and heavy radiation damage. Recently, we developed the frequency transmission electric-field (FTE) method, which facilitates the SEM observation of biological specimens in water without radiation damage. However, the signal detection system presents low sensitivity. Therefore, a high EB current is required to generate clear images, and thus reducing spatial resolution and inducing thermal damage to the samples. Here a high-sensitivity detection system is developed for the FTE method, which enhances the output signal amplitude by hundredfold. The detection signal was highly enhanced when voltage was applied to the metal layer on silicon nitride thin film. This enhancement reduced the EB current and improved the spatial resolution as well as the signal-to-noise ratio. The spatial resolution of a high-sensitive FTE system is 41 nm, which is considerably higher than previous FTE system. New FTE system can easily be utilised to examine various unstained biological specimens in water, such as living bacteria and viruses.

  8. Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals

    SciTech Connect (OSTI)

    Gambhir, Sanjiv; Pritha, Ray

    2015-07-14

    Novel double and triple fusion reporter gene constructs harboring distinct imagable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

  9. Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals

    DOE Patents [OSTI]

    Gambhir, Sanjiv; Pritha, Ray

    2011-06-07

    Novel double and triple fusion reporter gene constructs harboring distinct imagable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

  10. Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals

    DOE Patents [OSTI]

    Gambhir; Sanjiv , Pritha; Ray

    2009-04-28

    Novel double and triple fusion reporter gene constructs harboring distinct imageable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

  11. WAC - 232-12-064 Live Wildlife-Taking from the wild | Open Energy...

    Open Energy Info (EERE)

    32-12-064 Live Wildlife-Taking from the wild Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: WAC - 232-12-064 Live...

  12. ScienceLive chat page: on the future of fusion research | Princeton Plasma

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Physics Lab ScienceLive chat page: on the future of fusion research American Fusion News Category: U.S. Universities Link: ScienceLive chat page: on the future of fusion research

  13. Making the "Best Place to Live" Even Better

    Broader source: Energy.gov [DOE]

    For 2010, the appropriately named Eden Prairie, Minnesota was honored as the No. 1 Best Place to Live in the United States by Money Magazine. The highly-coveted civic title reflects many aspects of the family-friendly suburb of 62,000 located 16 miles from downtown Minneapolis, including the advantages of being the home to major employers like Fortune 500 trucking company C.H. Robinson and hearing-aid maker Starkey Labs. The city also hass many natural amenities like 17 lakes and parks with 125 miles of running, hiking, and biking trails.

  14. COLLOQUIUM: Living with Climate Change: The Road from Paris | Princeton

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Plasma Physics Lab April 20, 2016, 4:15pm to 5:30pm Colloquia MBG Auditorium (284 cap.) COLLOQUIUM: Living with Climate Change: The Road from Paris Dr. Dale Jamieson New York University Presentation: File WC20APR2016_DJamieson.pptx The twenty-first Conference of the Parties (COP21) of the United Nations Framework Convention on Climate Change (UNFCCC) in Paris ended with an agreement that some call "the world's greatest diplomatic success" while others insist it is "too

  15. Savannah River Site "Live Burn" Training Sharpens Skills | National Nuclear

    National Nuclear Security Administration (NNSA)

    Security Administration | (NNSA) Site "Live Burn" Training Sharpens Skills Tuesday, June 30, 2015 - 1:47pm Live Burn training As part of the training that equips them to ensure safe operations, Savannah River Site radiological protection (RP) and fire department personnel recently conducted their annual "Live Burn" training exercises that simulate fires in facilities with chemical and radiological contamination. The Live Burn exercise took place at the Martinez-Columbia

  16. Live wires: direct extracellular electron exchange for bioenergy and the bioremediation of energy-related contamination

    SciTech Connect (OSTI)

    Lovley, DR

    2011-12-01

    Microorganisms that can form direct electrical connections with insoluble minerals, electrodes, or other microorganisms can play an important role in some traditional as well as novel bioenergy strategies and can be helpful in the remediation of environmental contamination resulting from the use of more traditional energy sources. The surprising discovery that microorganisms in the genus Geobacter are capable of forming highly conductive networks of filaments that transfer electrons along their length with organic metallic-like conductivity, rather than traditional molecule to molecule electron exchange, provides an explanation for the ability of Geobacter species to grow in subsurface environments with insoluble Fe(III) oxides as the electron acceptor, and effectively remediate groundwater contaminated with hydrocarbon fuels or uranium and similar contaminants associated with the mining and processing of nuclear fuel. A similar organic metallic-like conductivity may be an important mechanism for microorganisms to exchange electrons in syntrophic associations, such as those responsible for the conversion of organic wastes to methane in anaerobic digesters, a proven bioenergy technology. Biofilms with conductivities rivaling those of synthetic polymers help Geobacter species generate the high current densities in microbial fuel cells producing electric current from organic compounds. Electron transfer in the reverse direction, i.e. from electrodes to microbes, is the basis for microbial electrosynthesis, in which microorganisms reduce carbon dioxide to fuels and other useful organic compounds with solar energy in a form of artificial photosynthesis that is more efficient and avoids many of the environmental sustainability concerns associated with biomass-based bioenergy strategies. The ability of Geobacter species to produce highly conductive electronic networks that function in water opens new possibilities in the emerging field of bioelectronics.

  17. Genomics Encyclopedia of Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB): a resource for microsymbiont genomes (2013 DOE JGI Genomics of Energy and Environment 8th Annual User Meeting)

    SciTech Connect (OSTI)

    Reeve, Wayne

    2013-03-01

    Wayne Reeve of Murdoch University on "Genomics Encyclopedia of Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB): a resource for microsymbiont genomes" at the 8th Annual Genomics of Energy & Environment Meeting on March 27, 2013 in Walnut Creek, Calif.

  18. Air Leakage and Air Transfer Between Garage and Living Space

    SciTech Connect (OSTI)

    Rudd, Armin

    2014-09-01

    This research project focused on evaluation of air transfer between the garage and living space in a single-family detached home constructed by a production homebuilder in compliance with the 2009 International Residential Code and the 2009 International Energy Conservation Code. The project gathered important information about the performance of whole-building ventilation systems and garage ventilation systems as they relate to minimizing flow of contaminated air from garage to living space. A series of 25 multi-point fan pressurization tests and additional zone pressure diagnostic testing characterized the garage and house air leakage, the garage-to-house air leakage, and garage and house pressure relationships to each other and to outdoors using automated fan pressurization and pressure monitoring techniques. While the relative characteristics of this house may not represent the entire population of new construction configurations and air tightness levels (house and garage) throughout the country, the technical approach was conservative and should reasonably extend the usefulness of the results to a large spectrum of house configurations from this set of parametric tests in this one house. Based on the results of this testing, the two-step garage-to-house air leakage test protocol described above is recommended where whole-house exhaust ventilation is employed.

  19. Methanobactin: a copper binding compound having antibiotic and antioxidant activity isolated from methanotrophic bacteria

    DOE Patents [OSTI]

    DiSpirito, Alan A.; Zahn, James A.; Graham, David W.; Kim, Hyung J.; Alterman, Michail; Larive, Cynthia

    2007-04-03

    A means and method for treating bacterial infection, providing antioxidant activity, and chelating copper using a copper binding compound produced by methanotrophic bacteria is described. The compound, known as methanobactin, is the first of a new class of antibiotics having gram-positive activity. Methanobactin has been sequenced, and its structural formula determined.

  20. Amoebae/bacteria consortia and uses for degrading wastes and contaminants

    DOE Patents [OSTI]

    Tyndall, R.L.

    1996-05-21

    A method is disclosed of altering trinitrotoluene. The steps include the following: providing an amoeba/bacteria consortium, particularly ATCC 40908 or a mutant which possesses all the identifying characteristics thereof; and contacting the consortium with trinitrotoluene to alter the trinitrotoluene.

  1. Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons

    SciTech Connect (OSTI)

    Wetmore, Kelly M.; Price, Morgan N.; Waters, Robert J.; Lamson, Jacob S.; He, Jennifer; Hoover, Cindi A.; Blow, Matthew J.; Bristow, James; Butland, Gareth; Arkin, Adam P.; Deutschbauer, Adam

    2015-05-12

    Transposon mutagenesis with next-generation sequencing (TnSeq) is a powerful approach to annotate gene function in bacteria, but existing protocols for TnSeq require laborious preparation of every sample before sequencing. Thus, the existing protocols are not amenable to the throughput necessary to identify phenotypes and functions for the majority of genes in diverse bacteria. Here, we present a method, random bar code transposon-site sequencing (RB-TnSeq), which increases the throughput of mutant fitness profiling by incorporating random DNA bar codes into Tn5 and mariner transposons and by using bar code sequencing (BarSeq) to assay mutant fitness. RB-TnSeq can be used with any transposon, and TnSeq is performed once per organism instead of once per sample. Each BarSeq assay requires only a simple PCR, and 48 to 96 samples can be sequenced on one lane of an Illumina HiSeq system. We demonstrate the reproducibility and biological significance of RB-TnSeq with Escherichia coli, Phaeobacter inhibens, Pseudomonas stutzeri, Shewanella amazonensis, and Shewanella oneidensis. To demonstrate the increased throughput of RB-TnSeq, we performed 387 successful genome-wide mutant fitness assays representing 130 different bacterium-carbon source combinations and identified 5,196 genes with significant phenotypes across the five bacteria. In P. inhibens, we used our mutant fitness data to identify genes important for the utilization of diverse carbon substrates, including a putative D-mannose isomerase that is required for mannitol catabolism. RB-TnSeq will enable the cost-effective functional annotation of diverse bacteria using mutant fitness profiling. A large challenge in microbiology is the functional assessment of the millions of uncharacterized genes identified by genome sequencing. Transposon mutagenesis coupled to next-generation sequencing (TnSeq) is a powerful approach to assign phenotypes and functions to genes

  2. Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wetmore, Kelly M.; Price, Morgan N.; Waters, Robert J.; Lamson, Jacob S.; He, Jennifer; Hoover, Cindi A.; Blow, Matthew J.; Bristow, James; Butland, Gareth; Arkin, Adam P.; et al

    2015-05-12

    Transposon mutagenesis with next-generation sequencing (TnSeq) is a powerful approach to annotate gene function in bacteria, but existing protocols for TnSeq require laborious preparation of every sample before sequencing. Thus, the existing protocols are not amenable to the throughput necessary to identify phenotypes and functions for the majority of genes in diverse bacteria. Here, we present a method, random bar code transposon-site sequencing (RB-TnSeq), which increases the throughput of mutant fitness profiling by incorporating random DNA bar codes into Tn5 and mariner transposons and by using bar code sequencing (BarSeq) to assay mutant fitness. RB-TnSeq can be used with anymore » transposon, and TnSeq is performed once per organism instead of once per sample. Each BarSeq assay requires only a simple PCR, and 48 to 96 samples can be sequenced on one lane of an Illumina HiSeq system. We demonstrate the reproducibility and biological significance of RB-TnSeq with Escherichia coli, Phaeobacter inhibens, Pseudomonas stutzeri, Shewanella amazonensis, and Shewanella oneidensis. To demonstrate the increased throughput of RB-TnSeq, we performed 387 successful genome-wide mutant fitness assays representing 130 different bacterium-carbon source combinations and identified 5,196 genes with significant phenotypes across the five bacteria. In P. inhibens, we used our mutant fitness data to identify genes important for the utilization of diverse carbon substrates, including a putative D-mannose isomerase that is required for mannitol catabolism. RB-TnSeq will enable the cost-effective functional annotation of diverse bacteria using mutant fitness profiling. A large challenge in microbiology is the functional assessment of the millions of uncharacterized genes identified by genome sequencing. Transposon mutagenesis coupled to next-generation sequencing (TnSeq) is a powerful approach to assign phenotypes and functions to genes. However, the current strategies for TnSeq are

  3. From Nanowires to Biofilms: An Exploration of Novel Mechanisms of Uranium Transformation Mediated by Geobacter Bacteria

    SciTech Connect (OSTI)

    REGUERA, GEMMA

    2014-01-16

    One promising strategy for the in situ bioremediation of radioactive groundwater contaminants that has been identified by the SBR Program is to stimulate the activity of dissimilatory metal-reducing microorganisms to reductively precipitate uranium and other soluble toxic metals. The reduction of U(VI) and other soluble contaminants by Geobacteraceae is directly dependent on the reduction of Fe(III) oxides, their natural electron acceptor, a process that requires the expression of Geobacter’s conductive pili (pilus nanowires). Expression of conductive pili by Geobacter cells leads to biofilm development on surfaces and to the formation of suspended biogranules, which may be physiological closer to biofilms than to planktonic cells. Biofilm development is often assumed in the subsurface, particularly at the matrix-well screen interface, but evidence of biofilms in the bulk aquifer matrix is scarce. Our preliminary results suggest, however, that biofilms develop in the subsurface and contribute to uranium transformations via sorption and reductive mechanisms. In this project we elucidated the mechanism(s) for uranium immobilization mediated by Geobacter biofilms and identified molecular markers to investigate if biofilm development is happening in the contaminated subsurface. The results provided novel insights needed in order to understand the metabolic potential and physiology of microorganisms with a known role in contaminant transformation in situ, thus having a significant positive impact in the SBR Program and providing novel concept to monitor, model, and predict biological behavior during in situ treatments.

  4. WATCH LIVE: Talking the Higgs Boson with CERN | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    WATCH LIVE: Talking the Higgs Boson with CERN WATCH LIVE: Talking the Higgs Boson with CERN September 13, 2012 - 2:33pm Addthis WATCH LIVE: Talking the Higgs Boson with CERN Michael Hess Michael Hess Former Digital Communications Specialist, Office of Public Affairs Where do I watch? To watch this livestream, go to energy.gov/live. Tune in to a special livestream at energy.gov/live on Friday, Sept. 14 at 10 a.m. ET for a science lecture about the Higgs boson with Joe Incandela, head spokesperson

  5. Firearm equipped with live round inhibiting means and method of making same

    DOE Patents [OSTI]

    Baehr, Donald G. (Albuquerque, NM)

    1990-01-01

    A firearm is disclosed having live round inhibiting means mounted in the barrel of the firearm which permits a blank cartridge to be loaded into a firearm and fired while preventing the loading and firing of a live round. The live round inhibiting means comprise shaft means mounted in the barrel of the firearm and which extends a sufficient length into the barrel at a point just beyond the chamber portion of the firearm to engage the bullet portion of a live round to prevent it from properly chambering, while permitting a blank cartridge to be loaded into the firearm and fired without engaging the live round-inhibiting shaft means.

  6. Firearm equipped with live round inhibiting means and method of making same

    DOE Patents [OSTI]

    Baehr, D.G.

    1990-11-13

    A firearm is disclosed having live round inhibiting means mounted in the barrel of the firearm which permits a blank cartridge to be loaded into a firearm and fired while preventing the loading and firing of a live round. The live round inhibiting means comprise shaft means mounted in the barrel of the firearm and which extends a sufficient length into the barrel at a point just beyond the chamber portion of the firearm to engage the bullet portion of a live round to prevent it from properly chambering, while permitting a blank cartridge to be loaded into the firearm and fired without engaging the live round-inhibiting shaft means. 9 figs.

  7. South Atlantic OCS area living marine resources study. Volume I: an investigation of live bottom habitats south of Cape Fear, North Carolina. Final report

    SciTech Connect (OSTI)

    Not Available

    1981-10-01

    The major objectives of this study were to (1) characterize benthic and nektonic communities associated with representative live bottom habitats on the continental shelf of the South Atlantic Bight, and (2) evaluate factors which might influence these communities, particularly the potential for impact by offshore oil and gas activities. The study areas include nine live bottom areas located off South Carolina, Georgia and Florida.

  8. Short-lived isomers in {sup 94}Rb

    SciTech Connect (OSTI)

    Tsekhanovich, I.; Dare, J. A.; Smith, A. G.; Varley, B. J.; Simpson, G. S.; Urban, W.; Soldner, T.; Jolie, J.; Linnemann, A.; Orlandi, R.; Smith, J. F.; Scherillo, A.; Rzaca-Urban, T.; Zlomaniec, A.; Dorvaux, O.; Gall, B. J. P.; Roux, B.

    2008-07-15

    The medium-spin structure of the neutron-rich, odd-odd nucleus {sup 94}Rb was studied by means of {gamma}-ray spectroscopy. Excited levels were populated in the neutron-induced fission of {sup 235}U and in the spontaneous fission of {sup 252}Cf and {sup 248}Cm. Two isomeric states were found at 1485.2 and 2074.8 keV with half-lives of 18 and 107 ns, respectively. The probable structures of the two isomers involve the fully aligned, proton-neutron configurations [{pi}(g{sub 9/2}) x {nu}(g{sub 7/2})]{sub 8{sup +}} and [{pi}(g{sub 9/2}) x {nu}(h{sub 11/2})]{sub 10{sup -}}, respectively. These new data give information on the single-particle energies in the region.

  9. Magnetic resonance imaging of living systems by remote detection

    DOE Patents [OSTI]

    Wemmer, David; Pines, Alexander; Bouchard, Louis; Xu, Shoujun; Harel, Elad; Budker, Dmitry; Lowery, Thomas; Ledbetter, Micah

    2013-10-29

    A novel approach to magnetic resonance imaging is disclosed. Blood flowing through a living system is prepolarized, and then encoded. The polarization can be achieved using permanent or superconducting magnets. The polarization may be carried out upstream of the region to be encoded or at the place of encoding. In the case of an MRI of a brain, polarization of flowing blood can be effected by placing a magnet over a section of the body such as the heart upstream of the head. Alternatively, polarization and encoding can be effected at the same location. Detection occurs at a remote location, using a separate detection device such as an optical atomic magnetometer, or an inductive Faraday coil. The detector may be placed on the surface of the skin next to a blood vessel such as a jugular vein carrying blood away from the encoded region.

  10. Living and Working Safely Around High-Voltage Power Lines.

    SciTech Connect (OSTI)

    United States. Bonneville Power Administration.

    2001-06-01

    High-voltage transmission lines can be just as safe as the electrical wiring in the homes--or just as dangerous. The crucial factor is ourselves: they must learn to behave safely around them. This booklet is a basic safety guide for those who live and work around power lines. It deals primarily with nuisance shocks due to induced voltages, and with potential electric shock hazards from contact with high-voltage lines. References on possible long-term biological effects of transmission lines are shown. In preparing this booklet, the Bonneville Power Administration has drawn on more than 50 years of experience with high-voltage transmission. BPA operates one of the world`s largest networks of long-distance, high-voltage lines. This system has more than 400 substations and about 15,000 miles of transmission lines, almost 4,400 miles of which are operated at 500,000 volts.

  11. Near-Term Climate Mitigation by Short-Lived Forcers

    SciTech Connect (OSTI)

    Smith, Steven J.; Mizrahi, Andrew H.

    2013-08-12

    Emissions reductions focused on anthropogenic climate forcing agents with relatively short atmospheric lifetimes such as methane (CH4) and black carbon (BC) have been suggested as a strategy to reduce the rate of climate change over the next several decades. We find that reductions of methane and BC would likely have only a modest impact on near-term climate warming. Even with maximally feasible reductions phased in from 2015 to 2035, global mean temperatures in 2050 are reduced by 0.16 °C, with an uncertainty range of 0.04-0.36°C, with the high end of this range only possible if total historical aerosol forcing is small. More realistic mitigation scenarios would likely provide a smaller climate benefit. The climate benefits from targeted reductions in short-lived forcing agents are smaller than previously estimated and are not substantially different in magnitude from the benefits due to a comprehensive climate policy.

  12. Air Leakage and Air Transfer Between Garage and Living Space

    SciTech Connect (OSTI)

    Rudd, A.

    2014-09-01

    This research project focused on evaluation of air transfer between the garage and living space in a single-family detached home constructed by a production homebuilder in compliance with the 2009 International Residential Code and the 2009 International Energy Conservation Code. The project gathered important information about the performance of whole-building ventilation systems and garage ventilation systems as they relate to minimizing flow of contaminated air from garage to living space. A series of 25 multi-point fan pressurization tests and additional zone pressure diagnostic testing characterized the garage and house air leakage, the garage-to-house air leakage, and garage and house pressure relationships to each other and to outdoors using automated fan pressurization and pressure monitoring techniques. While the relative characteristics of this house may not represent the entire population of new construction configurations and air tightness levels (house and garage) throughout the country, the technical approach was conservative and should reasonably extend the usefulness of the results to a large spectrum of house configurations from this set of parametric tests in this one house. Based on the results of this testing, the two-step garage-to-house air leakage test protocol described above is recommended where whole-house exhaust ventilation is employed. For houses employing whole-house supply ventilation (positive pressure) or balanced ventilation (same pressure effect as the Baseline condition), adherence to the EPA Indoor airPLUS house-to-garage air sealing requirements should be sufficient to expect little to no garage-to-house air transfer.

  13. Long-lived activation products in reactor materials

    SciTech Connect (OSTI)

    Evans, J.C.; Lepel, E.L.; Sanders, R.W.; Wilkerson, C.L.; Silker, W.; Thomas, C.W.; Abel, K.H.; Robertson, D.R.

    1984-08-01

    The purpose of this program was to assess the problems posed to reactor decommissioning by long-lived activation products in reactor construction materials. Samples of stainless steel, vessel steel, concrete, and concrete ingredients were analyzed for up to 52 elements in order to develop a data base of activatable major, minor, and trace elements. Large compositional variations were noted for some elements. Cobalt and niobium concentrations in stainless steel, for example, were found to vary by more than an order of magnitude. A thorough evaluation was made of all possible nuclear reactions that could lead to long lived activation products. It was concluded that all major activation products have been satisfactorily accounted for in decommissioning planning studies completed to date. A detailed series of calculations was carried out using average values of the measured compositions of the appropriate materials to predict the levels of activation products expected in reactor internals, vessel walls, and bioshield materials for PWR and BWR geometries. A comparison is made between calculated activation levels and regulatory guidelines for shallow land disposal according to 10 CFR 61. This analysis shows that PWR and BWR shroud material exceeds the Class C limits and is, therefore, generally unsuitable for near-surface disposal. The PWR core barrel material approaches the Class C limits. Most of the remaining massive components qualify as either Class A or B waste with the bioshield clearly Class A, even at the highest point of activation. Selected samples of activated steel and concrete were subjected to a limited radiochemical analysis program as a verification of the computer model. Reasonably good agreement with the calculations was obtained where comparison was possible. In particular, the presence of /sup 94/Nb in activated stainless steel at or somewhat above expected levels was confirmed.

  14. Experimental test of whether electrostatically charged micro-organisms and their spores contribute to the onset of arcs across vacuum gaps

    SciTech Connect (OSTI)

    Grisham, L. R.; Halle, A. von; Carpe, A. F.; Gilton, K. R.; Rossi, Guy; Stevenson, T. N.

    2013-12-15

    Recently it was proposed [L. R. Grisham et al. Phys. Plasmas 19, 023107 (2012)] that one of the initiators of vacuum voltage breakdown between conducting electrodes might be micro-organisms and their spores, previously deposited during exposure to air, which then become electrostatically charged when an electric potential is applied across the vacuum gap. This note describes a simple experiment to compare the number of voltage-conditioning pulses required to reach the nominal maximum operating voltage across a gap between two metallic conductors in a vacuum, comparing cases in which biological cleaning was done just prior to pump-down with cases where this was not done, with each case preceded by exposure to ambient air for three days. Based upon these results, it does not appear that air-deposited microbes and their spores constitute a major pathway for arc initiation, at least for exposure periods of a few days, and for vacuum gaps of a few millimeters, in the regime where voltage holding is usually observed to vary linearly with gap distance.

  15. Novel microorganism for selective separation of coal from ash and pyrite. Second quarterly technical progress report, 1 December 1993--28 February 1994

    SciTech Connect (OSTI)

    Misra, M.; Smith, R.W.; Raichur, A.M.

    1994-05-01

    The objective of this project is to study the effectiveness of a novel hydrophobic microorganism, Mycobacterium phlei (M. phlei), for the selective flocculation of coal from pyrite and ash forming minerals. During the reporting period, the hydrophobicity of different coal samples was studied both in the presence and absence of M. phlei cells. In the absence of M. Phlei, Illinois No. 6 and Pennsylvania No. 8 exhibited higher contact angles as compared to Kentucky No. 9 coal. All the coal samples exhibited a maximum in contact angle around pH 5--7, which roughly coincides with the iso-electric point (iep) of different coals studied in this investigation. In the presence of M. phlei, maximum contact angle shifted to lower pH range of 2--3 which coincides with the iep of the M. phlei. These measurements reinforce the notion that good flocculation of coal with M. phlei can be achieved around pH 2--3. The amount of soluble fraction released during rupturing of M. phlei cells was studied as a function of sonication time. The rupturing experiments showed that the whole cells (unruptured cells) contain nearly 40% by weight of soluble fractions. Also, during the reporting period, the fabrication of the counter-current flocculation device was completed.

  16. Experimental Test Of Whether Electrostatically Charged Micro-organisms And Their Spores Contribute To The Onset Of Arcs Across Vacuum Gaps

    SciTech Connect (OSTI)

    none,; Grisham, Larry R.

    2014-02-24

    Recently it was proposed [L.R. Grisham, A. vonHalle, A.F. Carpe, Guy Rossi, K.R. Gilton, E.D. McBride, E.P. Gilson, A. Stepanov, T.N. Stevenson, Physics of Plasma 19 023107 (2012)] that one of the initiators of vacuum voltage breakdown between condu cting electrodes might be micro-organisms and their spores, previously deposited during exposure to air, which tnen become electrostatically charged when an electric potential is applied across the vacuum gap. The note describes a simple experiment to compare the number of voltage-conditioning pulses required to reach the nominal maxium operating voltage across a gap between two metallic conductors in a vacuum, comparing cases in which biological cleaning was done just prior to pump-down with cases where this was not done, with each preceded by exposure to ambient air for three days. Based upon these results, it does not appear that air-deposited microbes and their spores constitute a major pathway for arc initiation, at least for exposure periods of a few days, and for vacuum gaps of a few millimeters, in the regime where voltage holding is usually observed to vary linearly with gap distance

  17. Pathway engineering and organism development for ethanol production from cellulosic biomass using thermophilic bacteria

    SciTech Connect (OSTI)

    Hogsett, D.A.L.; Klapatch, T.A.; Lynd, L.R.

    1995-12-01

    Thermophilic bacteria collectively exemplify organisms that produce both cellulose and ethanol while fermenting both the cellulose and hemicellulose components of biomass. As a result, thermophiles could be the basis for highly streamlined and cost-effective processes for production of renewable fuels and chemicals. Recent research results involving ethanol production from thermophilic bacteria will be presented, with a primary focus on work pursuant to molecularly-based pathway engineering to increase ethanol selectivity. Specifically, we will describe the restriction endonuclease systems operative in Clostridium thermocellum and C. thermosaccharolyticum, as well as efforts to document and improve transformation of these organisms and to clone key catabolic enzymes. In addition, selected results from fermentation studies will be presented as necessary in order to present a perspective on the status of thermophilic ethanol production.

  18. Turning Bacteria into Fuel: Cyanobacteria Designed for Solar-Powered Highly Efficient Production of Biofuels

    SciTech Connect (OSTI)

    2010-01-01

    Broad Funding Opportunity Announcement Project: ASU is engineering a type of photosynthetic bacteria that efficiently produce fatty acidsa fuel precursor for biofuels. This type of bacteria, called Synechocystis, is already good at converting solar energy and carbon dioxide (CO2) into a type of fatty acid called lauric acid. ASU has modified the organism so it continuously converts sunlight and CO2 into fatty acidsoverriding its natural tendency to use solar energy solely for cell growth and maximizing the solar-to-fuel conversion process. ASUs approach is different because most biofuels research focuses on increasing cellular biomass and not on excreting fatty acids. The project has also identified a unique way to convert the harvested lauric acid into a fuel that can be easily blended with existing transportation fuels.

  19. Cycling of DOC and DON by Novel Heterotrophic and Photoheterotrophic Bacteria in the Ocean: Final Report

    SciTech Connect (OSTI)

    Kirchman, David L

    2008-12-09

    The flux of dissolved organic matter (DOM) through aquatic bacterial communities is a major process in carbon cycling in the oceans and other aquatic systems. Our work addressed the general hypothesis that the phylogenetic make-up of bacterial communities and the abundances of key types of bacteria are important factors influencing the processing of DOM in aquatic ecosystems. Since most bacteria are not easily cultivated, the phylogenetic diversity of these microbes has to be assessed using culture-independent approaches. Even if the relevant bacteria were cultivated, their activity in the lab would likely differ from that under environmental conditions. This project found variation in DOM uptake by the major bacterial groups found in coastal waters. In brief, the data suggest substantial differences among groups in the use of high and molecular weight DOM components. It also made key discoveries about the role of light in affecting this uptake especially by cyanobacteria. In the North Atlantic Ocean, for example, over half of the light-stimulated uptake was by the coccoid cyanobacterium, Prochlorococcus, with the remaining uptake due to Synechococcus and other photoheterotrophic bacteria. The project also examined in detail the degradation of one organic matter component, chitin, which is often said to be the second most abundant compound in the biosphere. The findings of this project contribute to our understanding of DOM fluxes and microbial dynamics supported by those fluxes. It is possible that these findings will lead to improvements in models of the carbon cycle that have compartments for dissolved organic carbon (DOC), the largest pool of organic carbon in the oceans.

  20. Development of Microarrays-Based Metagenomics Technology for Monitoring Sulfate-Reducing Bacteria in Subsurface Environments

    SciTech Connect (OSTI)

    Cindy, Shi

    2015-07-17

    At the contaminated DOE sites, sulfate-reducing bacteria (SRB) are a significant population and play an important role in the microbial community during biostimulation for metal reduction. However, the diversity, structure and dynamics of SRB communities are poorly understood. Therefore, this project aims to use high throughput sequencing-based metagenomics technologies for characterizing the diversity, structure, functions, and activities of SRB communities by developing genomic and bioinformatics tools to link the SRB biodiversity with ecosystem functioning.

  1. Impact of elevated nitrate on sulfate-reducing bacteria: A comparative study of Desulfovibrio vulgaris

    SciTech Connect (OSTI)

    He, Q.; He, Z.; Joyner, D.C.; Joachimiak, M.; Price, M.N.; Yang, Z.K.; Yen, H.-C. B.; Hemme, C. L.; Chen, W.; Fields, M.; Stahl, D. A.; Keasling, J. D.; Keller, M.; Arkin, A. P.; Hazen, T. C.; Wall, J. D.; Zhou, J.

    2010-07-15

    Sulfate-reducing bacteria have been extensively studied for their potential in heavy-metal bioremediation. However, the occurrence of elevated nitrate in contaminated environments has been shown to inhibit sulfate reduction activity. Although the inhibition has been suggested to result from the competition with nitrate-reducing bacteria, the possibility of direct inhibition of sulfate reducers by elevated nitrate needs to be explored. Using Desulfovibrio vulgaris as a model sulfate-reducing bacterium, functional genomics analysis reveals that osmotic stress contributed to growth inhibition by nitrate as shown by the upregulation of the glycine/betaine transporter genes and the relief of nitrate inhibition by osmoprotectants. The observation that significant growth inhibition was effected by 70 mM NaNO{sub 3} but not by 70 mM NaCl suggests the presence of inhibitory mechanisms in addition to osmotic stress. The differential expression of genes characteristic of nitrite stress responses, such as the hybrid cluster protein gene, under nitrate stress condition further indicates that nitrate stress response by D. vulgaris was linked to components of both osmotic and nitrite stress responses. The involvement of the oxidative stress response pathway, however, might be the result of a more general stress response. Given the low similarities between the response profiles to nitrate and other stresses, less-defined stress response pathways could also be important in nitrate stress, which might involve the shift in energy metabolism. The involvement of nitrite stress response upon exposure to nitrate may provide detoxification mechanisms for nitrite, which is inhibitory to sulfate-reducing bacteria, produced by microbial nitrate reduction as a metabolic intermediate and may enhance the survival of sulfate-reducing bacteria in environments with elevated nitrate level.

  2. A Scanning Auger Microprobe analysis of corrosion products associated with sulfate reducing bacteria

    SciTech Connect (OSTI)

    Sadowski, R.A.; Chen, G.; Clayton, C.R.; Kearns, J.R.; Gillow, J.B.; Francis, A.J.

    1995-03-01

    A Scanning Auger Microprobe analysis was performed on the corrosion products of an austenitic AISI type 304 SS after a potentiostatic polarization of one volt for ten minutes in a modified Postgate`s C media containing sulfate reducing bacteria. The corrosion products were characterized and mapped in local regions where pitting was observed. A critical evaluation of the applicability of this technique for the examination of microbially influenced corrosion (MIC) is presented.

  3. Stimulate Bacteria to Stop Chromium in Groundwater | U.S. DOE Office of

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Science (SC) Stimulate Bacteria to Stop Chromium in Groundwater Biological and Environmental Research (BER) BER Home About Research Facilities Science Highlights Searchable Archive of BER Highlights External link Benefits of BER Funding Opportunities Biological & Environmental Research Advisory Committee (BERAC) Community Resources Contact Information Biological and Environmental Research U.S. Department of Energy SC-23/Germantown Building 1000 Independence Ave., SW Washington, DC 20585

  4. Structure of the DUF2233 Domain in Bacteria and the Stuttering-associated

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    UCE Glycoprotein | Stanford Synchrotron Radiation Lightsource the DUF2233 Domain in Bacteria and the Stuttering-associated UCE Glycoprotein Wednesday, July 31, 2013 UCE figure DUF2233, a Domain of Unknown Function (DUF), is present in ~1200 bacterial and several viral and eukaryotic proteins. DUF2233 has been identified in proteins ranging in size from ~300-2000 residues. The 515 amino acid mammalian transmembrane glycoprotein α-N-acetylglucosamine-1-phosphodiester N-acetylglucosaminidase

  5. Improved oil recovery using bacteria isolated from North Sea petroleum reservoirs

    SciTech Connect (OSTI)

    Davey, R.A.; Lappin-Scott, H.

    1995-12-31

    During secondary oil recovery, water is injected into the formation to sweep out the residual oil. The injected water, however, follows the path of least resistance through the high-permeability zones, leaving oil in the low-permeability zones. Selective plugging of these their zones would divert the waterflood to the residual oil and thus increase the life of the well. Bacteria have been suggested as an alternative plugging agent to the current method of polymer injection. Starved bacteria can penetrate deeply into rock formations where they attach to the rock surfaces, and given the right nutrients can grow and produce exo-polymer, reducing the permeability of these zones. The application of microbial enhanced oil recovery has only been applied to shallow, cool, onshore fields to date. This study has focused on the ability of bacteria to enhance oil recovery offshore in the North Sea, where the environment can be considered extreme. A screen of produced water from oil reservoirs (and other extreme subterranean environments) was undertaken, and two bacteria were chosen for further work. These two isolates were able to grow and survive in the presence of saline formation waters at a range of temperatures above 50{degrees}C as facultative anaerobes. When a solution of isolates was passed through sandpacks and nutrients were added, significant reductions in permeabilities were achieved. This was confirmed in Clashach sandstone at 255 bar, when a reduction of 88% in permeability was obtained. Both isolates can survive nutrient starvation, which may improve penetration through the reservoir. Thus, the isolates show potential for field trials in the North Sea as plugging agents.

  6. Environmental diagnostic analysis of ground water bacteria and their involvement in utilization of aromatic compounds

    SciTech Connect (OSTI)

    Wear, J.E. Jr.

    1993-05-01

    The objective of this study was to examine the hypothesis that select functional groups of bacteria from pristine sites have an innate ability to degrade synthetic aromatics that often contaminate groundwater environments,due to exposure to naturally occurring recalcitrant aromatics in their environment. This study demonstrates that subsurface microbial communities are capable of utilizing lignin and humic acid breakdown products. Utilizers of these compounds were found to be present in most all the wells tested. Even the deepest aquifer tested had utilizers present for all six of the aromatics tested. Highest counts for the aromatics tested were observed with the naturally occurring breakdown products of either lignin or humic acid. Carboxylic acids were found to be an important sole carbon source for groundwater bacteria possibly explained by the fact that they are produced by the oxidative cleavage of aromatic ring structures. The carbohydrate sole carbon sources that demonstrated the greatest densities were ones commonly associated with humics. This study indicates that utilization of naturally occurring aromatic compounds in the subsurface is an important nutritional source for groundwater bacteria. In addition, it suggests that adaptation to naturally occurring recalcitrant substrates is the origin of degradative pathways for xenobiotic compounds with analogous structure. This work has important implications for in situ bioremediation as a method of environmental cleanup.

  7. Isolation of butyrate-utilizing bacteria from thermophilic and mesophilic methane-producing ecosystems

    SciTech Connect (OSTI)

    Henson, J.M.

    1983-01-01

    The ability of various ecosystems to convert butyrate to methane was studied in order to isolate the bacteria responsible for the conversion. When thermophilic digester sludge was enriched with butyrate, methane was produced without a lag period. Marine sediments enriched with butyrate required a 2-week incubation period before methanogenesis began. A thermophilic digester was studied in more detail and found by most-probable-number enumeration to have ca. 5 x 10/sup 6/ butyrate-utilizing bactera/ml of sludge. A thermophilic butyrate-utilizing bacterium was isolated in coculture with Methanobacterium thermoautotrophicum and a Methanosarcina sp. This bacterium was a gram-negative, slightly curved rod that occurred singly, was nonmotile, and did not appear to produce spores. The thermophilic digester was infused with butyrate at the rate of 10 ..mu..moles/ml of sludge per day. Biogas production increased by 150%, with the percentage of methane increasing from 58% to 68%. Acetate, propionate, and butyrate did not accumulate. Butyrate-utilizing enrichments from mesophilic ecosystems were used in obtaining cocultures of butyrate-utilizing bacteria. These cocultures served as inocula for attempts to isolate pure cultures of butyrate-utilizing bacteria by use of hydrogenase-containing membrane fragments of Escherichia coli. After a 3-week incubation period, colonies appeared only in inoculated tubes that contained membrane fragments and butyrate.

  8. Search for Long-Lived Particles ine+e-Collisions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lees, J. P.; Poireau, V.; Tisserand, V.; Grauges, E.; Palano, A.; Eigen, G.; Stugu, B.; Brown, D. N.; Kerth, L. T.; Kolomensky, Yu. G.; et al

    2015-04-29

    We present a search for a neutral, long-lived particle L that is produced in e+e- collisions and decays at a significant distance from the e+e- interaction point into various flavor combinations of two oppositely charged tracks. The analysis uses an e+e- data sample with a luminosity of 489.1 fb-1 collected by the BABAR detector at the γ (4S), γ (3S), and γ (2S) resonances and just below the γ (4S). Fitting the two-track mass distribution in search of a signal peak, we do not observe a significant signal, and set 90% confidence level upper limits on the product of themore »L production cross section, branching fraction, and reconstruction efficiency for six possible two-body L decay modes as a function of the L mass. The efficiency is given for each final state as a function of the mass, lifetime, and transverse momentum of the candidate, allowing application of the upper limits to any production model. In addition, upper limits are provided on the branching fraction B(B→XsL), where Xs is a strange hadronic system.« less

  9. Search for Long-Lived Particles ine+e-Collisions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lees, J. P.; Poireau, V.; Tisserand, V.; Grauges, E.; Palano, A.; Eigen, G.; Stugu, B.; Brown, D. N.; Kerth, L. T.; Kolomensky, Yu. G.; et al

    2015-04-29

    We present a search for a neutral, long-lived particle L that is produced in e+e- collisions and decays at a significant distance from the e+e- interaction point into various flavor combinations of two oppositely charged tracks. The analysis uses an e+e- data sample with a luminosity of 489.1 fb-1 collected by the BABAR detector at the γ (4S), γ (3S), and γ (2S) resonances and just below the γ (4S). Fitting the two-track mass distribution in search of a signal peak, we do not observe a significant signal, and set 90% confidence level upper limits on the product of themore » L production cross section, branching fraction, and reconstruction efficiency for six possible two-body L decay modes as a function of the L mass. The efficiency is given for each final state as a function of the mass, lifetime, and transverse momentum of the candidate, allowing application of the upper limits to any production model. In addition, upper limits are provided on the branching fraction B(B→XsL), where Xs is a strange hadronic system.« less

  10. One Thing Leads to Another: How NETL Research Saves Lives | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy One Thing Leads to Another: How NETL Research Saves Lives One Thing Leads to Another: How NETL Research Saves Lives August 8, 2013 - 10:57am Addthis One Thing Leads to Another: How NETL Research Saves Lives Learn More The coronary stent was developed as part of NETL's Technology Transfer program. NETL's technology portfolio contains a broad range of innovations that have resulted from research in areas such as carbon capture and sequestration, mercury capture, fuel cells, sensors and

  11. Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Innovation and Deployment | Department of Energy Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and Deployment Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and Deployment October 19, 2011 - 3:00pm Addthis Washington, D.C. -- Thursday, October 20th, Richard Kauffman, Senior Adviser to the Secretary of Energy, will host the Department of Energy's third "Energy Matters" live chat. Kauffman will discuss the challenges and

  12. Upcoming Live Discussion on Energy 101: Fuel Cells | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Live Discussion on Energy 101: Fuel Cells Upcoming Live Discussion on Energy 101: Fuel Cells January 10, 2014 - 12:00am Addthis Join the Energy Department at 2:00 p.m. ET on Thursday, January 16 for the first Energy 101 Google+ Hangout, which will focus on fuel cells.Submit questions for fuel cell experts in advance and during the hangout, and watch the discussion live on energy.gov/live or the Energy Department's Google+ page.Read more about this event and how to submit questions. Addthis

  13. Live Webinar on Better Buildings Case Competition: Energy Efficiency in the Restaurant Franchise Model Case Study

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webinar titled "A Side of Savings: Energy Efficiency in the Restaurant Franchise Model Case Study."

  14. WPN 06-6: Weatherization Whole House Assessment Training Live Feed DVD

    Broader source: Energy.gov [DOE]

    To issue the Weatherization Whole-House Training Assessment Training Live Feed DVD to all grantees for use in the Weatherization Assistance Program (WAP).

  15. #AskEnergy: Join the Live Q&A on Wind Energy | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AskEnergy: Join the Live Q&A on Wind Energy #AskEnergy: Join the Live Q&A on Wind Energy August 18, 2014 - 8:52am Q&A Submit your wind energy questions for tomorrow's live Twitter Q&A now! Ask Us Addthis Have questions about wind energy? Experts from the Energy Department are answering your questions about wind during a live Twitter Q&A on Tuesday, August 19, at 1 p.m. ET. | Graphic by <a href="/node/379579">Sarah Gerrity</a>, Energy Department. Have

  16. Live Webinar on Better Buildings Case Competition: Taking Commercial PACE Financing to Scale, a Case Study

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webinar titled "A Side of Savings: Energy Efficiency in the Restaurant Franchise Model Case Study."

  17. LiveChat Wed, 11/16, 2 pm ET: Industrial Energy Efficiency |...

    Energy Savers [EERE]

    Kathleen Hogan, Deputy Assistant Secretary for Energy Efficiency, for a live discussion about how businesses are lowering their energy consumption, and how the resulting energy ...

  18. Efficient Living in a Small House: Could You Make the Switch?

    Broader source: Energy.gov [DOE]

    The Small House Movement encourages homeowners to reject the huge homes that dominate many suburban neighborhoods and embrace smaller homes and simpler living.

  19. Reprogramming Bacteria to Seek and Destroy Small Molecules (JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)

    ScienceCinema (OSTI)

    Gallivan, Justin [Emory University

    2013-01-22

    Justin Gallivan, of Emory University presents a talk titled "Reprogramming Bacteria to Seek and Destroy Small Molecules" at the JGI User 7th Annual Genomics of Energy & Environment Meeting on March 21, 2012 in Walnut Creek, Calif

  20. Liquid Fuel From Bacteria: Engineering Ralstonia eutropha for Production of Isobutanol (IBT) Motor Fuel from CO2, Hydrogen, and Oxygen

    SciTech Connect (OSTI)

    2010-07-15

    Electrofuels Project: MIT is using solar-derived hydrogen and common soil bacteria called Ralstonia eutropha to turn carbon dioxide (CO2) directly into biofuel. This bacteria already has the natural ability to use hydrogen and CO2 for growth. MIT is engineering the bacteria to use hydrogen to convert CO2 directly into liquid transportation fuels. Hydrogen is a flammable gas, so the MIT team is building an innovative reactor system that will safely house the bacteria and gas mixture during the fuel-creation process. The system will pump in precise mixtures of hydrogen, oxygen, and CO2, and the online fuel-recovery system will continuously capture and remove the biofuel product.

  1. Reprogramming Bacteria to Seek and Destroy Small Molecules (JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)

    SciTech Connect (OSTI)

    Gallivan, Justin [Emory University] [Emory University

    2012-03-21

    Justin Gallivan, of Emory University presents a talk titled "Reprogramming Bacteria to Seek and Destroy Small Molecules" at the JGI User 7th Annual Genomics of Energy & Environment Meeting on March 21, 2012 in Walnut Creek, Calif

  2. Bioaccumulation of heavy metals in macroinvertebrates living in stormwater wetlands

    SciTech Connect (OSTI)

    Karouna, N.K.; Sparling, D.W.

    1995-12-31

    The design of stormwater wetlands and ponds as wildlife habitats has prompted concern over the potential uptake of runoff contaminants by aquatic fauna. Stormwater wetlands provide a diverse array of habitat for aquatic macroinvertebrates. The importance of macroinvertebrates in aquatic communities has been well documented. Aquatic macroinvertebrates also serve as a major food source of many aquatic vertebrates, including fish and birds. The objectives of the study were to: (1) examine the responses of the macroinvertebrate community to water and sediment concentrations of heavy metals, and other water quality parameters; (2) determine whether macroinvertebrates living in stormwater wetlands bioaccumulate significant concentrations of heavy metals; (3) relate the concentrations of heavy metals in sediment, water and macroinvertebrates to land use in the surrounding watershed; (4) determine sediment and water toxicity to macroinvertebrates. Twenty stormwater wetlands, representing four land uses commercial, residential, highway and control, were monitored in this study. Water quality parameters, including pH, DO, turbidity, conductivity, hardness and metal concentrations were monitored bi-weekly for six months. Sediment samples were collected three times during the same period. Macroinvertebrate communities were sampled during alternate weeks after water collections. Ten-day sediment bioassays were conducted using the amphipod Hyalella azteca. Preliminary data analyses have indicated no significant difference in sediment and water metal concentrations between land uses. However, Zn concentrations in macroinvertebrates were significantly higher (p < 0.05) in wetlands serving commercial watersheds than in those serving the remaining three land uses. No differences have been detected in composition of invertebrate communities due to land use category.

  3. MOLECULAR APPROACHES FOR IN SITU IDENTIFCIATION OF NITRATE UTILIZATION BY MARINE BACTERIA AND PHYTOPLANKTON

    SciTech Connect (OSTI)

    Frischer, Marc E.; Verity, Peter G.; Gilligan, Mathew R.; Bronk, Deborah A.; Zehr, Jonathan P.; Booth, Melissa G.

    2013-09-12

    Traditionally, the importance of inorganic nitrogen (N) for the nutrition and growth of marine phytoplankton has been recognized, while inorganic N utilization by bacteria has received less attention. Likewise, organic N has been thought to be important for heterotrophic organisms but not for phytoplankton. However, accumulating evidence suggests that bacteria compete with phytoplankton for nitrate (NO3-) and other N species. The consequences of this competition may have a profound effect on the flux of N, and therefore carbon (C), in ocean margins. Because it has been difficult to differentiate between N uptake by heterotrophic bacterioplankton versus autotrophic phytoplankton, the processes that control N utilization, and the consequences of these competitive interactions, have traditionally been difficult to study. Significant bacterial utilization of DIN may have a profound effect on the flux of N and C in the water column because sinks for dissolved N that do not incorporate inorganic C represent mechanisms that reduce the atmospheric CO2 drawdown via the ?biological pump? and limit the flux of POC from the euphotic zone. This project was active over the period of 1998-2007 with support from the DOE Biotechnology Investigations ? Ocean Margins Program (BI-OMP). Over this period we developed a tool kit of molecular methods (PCR, RT-PCR, Q-PCR, QRT-PCR, and TRFLP) and combined isotope mass spectrometry and flow-cytometric approaches that allow selective isolation, characterization, and study of the diversity and genetic expression (mRNA) of the structural gene responsible for the assimilation of NO3- by heterotrophic bacteria (nasA). As a result of these studies we discovered that bacteria capable of assimilating NO3- are ubiquitous in marine waters, that the nasA gene is expressed in these environments, that heterotrophic bacteria can account for a significant fraction of total DIN uptake in different ocean margin systems, that the expression of nasA is

  4. Palladium nanoparticles produced by fermentatively cultivated bacteria as catalyst for diatrizoate removal with biogenic hydrogen

    SciTech Connect (OSTI)

    Hennebel, T.; Fitts, J.; Nevel, S. V.; Verschuere, S.; DeCorte, S.; DeGusseme, B.; Cuvelier, C.; vanderLelie, D.; Boon, N.; Verstraete, W.

    2011-05-17

    A new biological inspired method to produce nanopalladium is the precipitation of Pd on a bacterium, i.e., bio-Pd. This bio-Pd can be applied as catalyst in dehalogenation reactions. However, large amounts of hydrogen are required as electron donor in these reactions resulting in considerable costs. This study demonstrates how bacteria, cultivated under fermentative conditions, can be used to reductively precipitate bio-Pd catalysts and generate the electron donor hydrogen. In this way, one could avoid the costs coupled to hydrogen supply. The catalytic activities of Pd(0) nanoparticles produced by different strains of bacteria (bio-Pd) cultivated under fermentative conditions were compared in terms of their ability to dehalogenate the recalcitrant aqueous pollutants diatrizoate and trichloroethylene. While all of the fermentative bio-Pd preparations followed first order kinetics in the dehalogenation of diatrizoate, the catalytic activity differed systematically according to hydrogen production and starting Pd(II) concentration in solution. Batch reactors with nanoparticles formed by Citrobacter braakii showed the highest diatrizoate dehalogenation activity with first order constants of 0.45 {+-} 0.02 h{sup -1} and 5.58 {+-} 0.6 h{sup -1} in batches with initial concentrations of 10 and 50 mg L{sup -1} Pd, respectively. Nanoparticles on C. braakii, used in a membrane bioreactor treating influent containing 20 mg L{sup -1} diatrizoate, were capable of dehalogenating 22 mg diatrizoate mg{sup -1} Pd over a period of 19 days before bio-Pd catalytic activity was exhausted. This study demonstrates the possibility to use the combination of Pd(II), a carbon source and bacteria under fermentative conditions for the abatement of environmental halogenated contaminants.

  5. Exploration of Simple Analytical Approaches for Rapid Detection of Pathogenic Bacteria

    SciTech Connect (OSTI)

    Salma Rahman

    2005-12-17

    Many of the current methods for pathogenic bacterial detection require long sample-preparation and analysis time, as well as complex instrumentation. This dissertation explores simple analytical approaches (e.g., flow cytometry and diffuse reflectance spectroscopy) that may be applied towards ideal requirements of a microbial detection system, through method and instrumentation development, and by the creation and characterization of immunosensing platforms. This dissertation is organized into six sections. In the general Introduction section a literature review on several of the key aspects of this work is presented. First, different approaches for detection of pathogenic bacteria will be reviewed, with a comparison of the relative strengths and weaknesses of each approach, A general overview regarding diffuse reflectance spectroscopy is then presented. Next, the structure and function of self-assembled monolayers (SAMs) formed from organosulfur molecules at gold and micrometer and sub-micrometer patterning of biomolecules using SAMs will be discussed. This section is followed by four research chapters, presented as separate manuscripts. Chapter 1 describes the efforts and challenges towards the creation of imunosensing platforms that exploit the flexibility and structural stability of SAMs of thiols at gold. 1H, 1H, 2H, 2H-perfluorodecyl-1-thiol SAM (PFDT) and dithio-bis(succinimidyl propionate)-(DSP)-derived SAMs were used to construct the platform. Chapter 2 describes the characterization of the PFDT- and DSP-derived SAMs, and the architectures formed when it is coupled to antibodies as well as target bacteria. These studies used infrared reflection spectroscopy (IRS), X-ray photoelectron spectroscopy (XPS), and electrochemical quartz crystal microbalance (EQCM), Chapter 3 presents a new sensitive, and portable diffuse reflection based technique for the rapid identification and quantification of pathogenic bacteria. Chapter 4 reports research efforts in the

  6. Soft inertial microfluidics for high throughput separation of bacteria from human blood cells

    SciTech Connect (OSTI)

    Wu, Zhigang; Willing, Ben; Bjerketorp, Joakim; Jansson, Janet K.; Hjort, Klas

    2009-01-05

    We developed a new approach to separate bacteria from human blood cells based on soft inertial force induced migration with flow defined curved and focused sample flow inside a microfluidic device. This approach relies on a combination of an asymmetrical sheath flow and proper channel geometry to generate a soft inertial force on the sample fluid in the curved and focused sample flow segment to deflect larger particles away while the smaller ones are kept on or near the original flow streamline. The curved and focused sample flow and inertial effect were visualized and verified using a fluorescent dye primed in the device. First the particle behavior was studied in detail using 9.9 and 1.0 {micro}m particles with a polymer-based prototype. The prototype device is compact with an active size of 3 mm{sup 2}. The soft inertial effect and deflection distance were proportional to the fluid Reynolds number (Re) and particle Reynolds number (Re{sub p}), respectively. We successfully demonstrated separation of bacteria (Escherichia coli) from human red blood cells at high cell concentrations (above 10{sup 8}/mL), using a sample flow rate of up to 18 {micro}L/min. This resulted in at least a 300-fold enrichment of bacteria at a wide range of flow rates with a controlled flow spreading. The separated cells were proven to be viable. Proteins from fractions before and after cell separation were analyzed by gel electrophoresis and staining to verify the removal of red blood cell proteins from the bacterial cell fraction. This novel microfluidic process is robust, reproducible, simple to perform, and has a high throughput compared to other cell sorting systems. Microfluidic systems based on these principles could easily be manufactured for clinical laboratory and biomedical applications.

  7. Molecular Regulation of Photosynthetic Carbon Dioxide Fixation in Nonsulfur Purple Bacteria

    SciTech Connect (OSTI)

    Tabita, Fred Robert

    2015-12-01

    The overall objective of this project is to determine the mechanism by which a transcriptional activator protein affects CO2 fixation (cbb) gene expression in nonsulfur purple photosynthetic bacteria, with special emphasis to Rhodobacter sphaeroides and with comparison to Rhodopseudomonas palustris. These studies culminated in several publications which indicated that additional regulators interact with the master regulator CbbR in both R. sphaeroides and R. palustris. In addition, the interactive control of the carbon and nitrogen assimilatory pathways was studied and unique regulatory signals were discovered.

  8. Thiol-facilitated cell export and desorption of methylmercury by anaerobic bacteria

    SciTech Connect (OSTI)

    Lin, Hui; Lu, Xia; Liang, Liyuan; Gu, Baohua

    2015-09-04

    Neurotoxic methylmercury (MeHg), formed by anaerobic bacteria, is shown to be rapidly excreted from the cell, but the mechanism of this process is unclear. Using both Geobacter sulfurreducens PCA and Desulfovibrio desulfuricans ND132 strains, we investigated the factors affecting export and distribution of MeHg in mercury methylation and MeHg sorption-desorption assays. Thiols, such as cysteine, were found to greatly facilitate desorption and export of MeHg, particularly by PCA cells. However, in cysteine-free assays (4 h) >90% of the synthesized MeHg was associated with PCA, among which ~73% was sorbed on the cell surface and 19% remained inside the cells. In comparison, a majority of the MeHg (70%) was exported by ND132, leaving ~20% of the MeHg sorbed on the surface and 10% inside the cells. When MeHg was added directly to the cell suspensions, ND132 adsorbed much lower MeHg but took up more MeHg inside cells than PCA did. These results demonstrate that MeHg export is bacteria strain-specific, time dependent, and is influenced by thiols, implicating important roles of ligand complexation in facilitating MeHg production and mobilization in the environment.

  9. Discovery of novel plant interaction determinants from the genomes of 163 root nodule bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Seshadri, Rekha; Reeve, Wayne G.; Ardley, Julie K.; Tennessen, Kristin; Woyke, Tanja; Kyrpides, Nikos C.; Ivanova, Natalia N.

    2015-11-20

    Root nodule bacteria (RNB) or “rhizobia” are a type of plant growth promoting bacteria, typified by their ability to fix nitrogen for their plant host, fixing nearly 65% of the nitrogen currently utilized in sustainable agricultural production of legume crops and pastures. In this study, we sequenced the genomes of 110 RNB from diverse hosts and biogeographical regions, and undertook a global exploration of all available RNB genera with the aim of identifying novel genetic determinants of symbiotic association and plant growth promotion. Specifically, we performed a subtractive comparative analysis with non-RNB genomes, employed relevant transcriptomic data, and leveraged phylogeneticmore » distribution patterns and sequence signatures based on known precepts of symbioticand host-microbe interactions. A total of 184 protein families were delineated, including known factors for nodulation and nitrogen fixation, and candidates with previously unexplored functions, for which a role in host-interaction, -regulation, biocontrol, and more, could be posited. Lastly, these analyses expand our knowledge of the RNB purview and provide novel targets for strain improvement in the ultimate quest to enhance plant productivity and agricultural sustainability.« less

  10. Discovery of novel plant interaction determinants from the genomes of 163 root nodule bacteria

    SciTech Connect (OSTI)

    Seshadri, Rekha; Reeve, Wayne G.; Ardley, Julie K.; Tennessen, Kristin; Woyke, Tanja; Kyrpides, Nikos C.; Ivanova, Natalia N.

    2015-11-20

    Root nodule bacteria (RNB) or “rhizobia” are a type of plant growth promoting bacteria, typified by their ability to fix nitrogen for their plant host, fixing nearly 65% of the nitrogen currently utilized in sustainable agricultural production of legume crops and pastures. In this study, we sequenced the genomes of 110 RNB from diverse hosts and biogeographical regions, and undertook a global exploration of all available RNB genera with the aim of identifying novel genetic determinants of symbiotic association and plant growth promotion. Specifically, we performed a subtractive comparative analysis with non-RNB genomes, employed relevant transcriptomic data, and leveraged phylogenetic distribution patterns and sequence signatures based on known precepts of symbioticand host-microbe interactions. A total of 184 protein families were delineated, including known factors for nodulation and nitrogen fixation, and candidates with previously unexplored functions, for which a role in host-interaction, -regulation, biocontrol, and more, could be posited. Lastly, these analyses expand our knowledge of the RNB purview and provide novel targets for strain improvement in the ultimate quest to enhance plant productivity and agricultural sustainability.

  11. Lubricating bacteria model for the growth of bacterial colonies exposed to ultraviolet radiation

    SciTech Connect (OSTI)

    Zhang Shengli; Zhang Lei; Liang Run; Zhang Erhu; Liu Yachao; Zhao Shumin

    2005-11-01

    In this paper, we study the morphological transition of bacterial colonies exposed to ultraviolet radiation by modifying the bacteria model proposed by Delprato et al. Our model considers four factors: the lubricant fluid generated by bacterial colonies, a chemotaxis initiated by the ultraviolet radiation, the intensity of the ultraviolet radiation, and the bacteria's two-stage destruction rate with given radiation intensities. Using this modified model, we simulate the ringlike pattern formation of the bacterial colony exposed to uniform ultraviolet radiation. The following is shown. (1) Without the UV radiation the colony forms a disklike pattern and reaches a constant front velocity. (2) After the radiation is switched on, the bacterial population migrates to the edge of the colony and forms a ringlike pattern. As the intensity of the UV radiation is increased the ring forms faster and the outer velocity of the colony decreases. (3) For higher radiation intensities the total population decreases, while for lower intensities the total population increases initially at a small rate and then decreases. (4) After the UV radiation is switched off, the bacterial population grows both outward as well as into the inner region, and the colony's outer front velocity recovers to a constant value. All these results agree well with the experimental observations [Phys. Rev. Lett. 87, 158102 (2001)]. Along with the chemotaxis, we find that lubricant fluid and the two-stage destruction rate are critical to the dynamics of the growth of the bacterial colony when exposed to UV radiation, and these were not previously considered.

  12. Nitrous oxide production and methane oxidation by different ammonia-oxidizing bacteria

    SciTech Connect (OSTI)

    Jiang, Q.Q.; Bakken, L.R.

    1999-06-01

    Ammonia-oxidizing bacteria (AOB) are thought to contribute significantly to N{sub 2}O production and methane oxidation in soils. Most knowledge derives from experiments with Nitrosomonas europaea, which appears to be of minor importance in most soils compared to Nitrosospira spp. The authors have conducted a comparative study of levels of aerobic N{sub 2}O production in six phylogenetically different Nitrosospira strains newly isolated from soils and in two N. europaea and Nitrosospira multiformis type strains. The fraction of oxidized ammonium released as N{sub 2}O during aerobic growth was remarkably constant for all the Nitrosospira strains, irrespective of the substrate supply (urea versus ammonium), the pH, or substrate limitation. N. europaea and Nitrosospira multiformis released similar fractions of N{sub 2}O when they were supplied with ample amounts of substrates, but the fractions rose sharply when they were restricted by a low pH or substrate limitation. Phosphate buffer doubled the N{sub 2}O release for all types of AOB. No detectable oxidation of atmospheric methane was detected. Calculations based on detection limits as well as data in the literature on CH{sub 4} oxidation by AOB bacteria prove that none of the tested strains contribute significantly to the oxidation of atmospheric CH{sub 4} in soils.

  13. Thiol-facilitated cell export and desorption of methylmercury by anaerobic bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lin, Hui; Lu, Xia; Liang, Liyuan; Gu, Baohua

    2015-09-04

    Neurotoxic methylmercury (MeHg), formed by anaerobic bacteria, is shown to be rapidly excreted from the cell, but the mechanism of this process is unclear. Using both Geobacter sulfurreducens PCA and Desulfovibrio desulfuricans ND132 strains, we investigated the factors affecting export and distribution of MeHg in mercury methylation and MeHg sorption-desorption assays. Thiols, such as cysteine, were found to greatly facilitate desorption and export of MeHg, particularly by PCA cells. However, in cysteine-free assays (4 h) >90% of the synthesized MeHg was associated with PCA, among which ~73% was sorbed on the cell surface and 19% remained inside the cells. Inmore » comparison, a majority of the MeHg (70%) was exported by ND132, leaving ~20% of the MeHg sorbed on the surface and 10% inside the cells. When MeHg was added directly to the cell suspensions, ND132 adsorbed much lower MeHg but took up more MeHg inside cells than PCA did. These results demonstrate that MeHg export is bacteria strain-specific, time dependent, and is influenced by thiols, implicating important roles of ligand complexation in facilitating MeHg production and mobilization in the environment.« less

  14. Size tunable elemental copper nanoparticles: extracellular synthesis by thermoanaerobic bacteria and capping molecules

    SciTech Connect (OSTI)

    Jang, Gyoung Gug; Jacobs, Christopher B; Gresback, Ryan G; Ivanov, Ilia N; Meyer III, Harry M; Kidder, Michelle; Joshi, Pooran C; Jellison Jr, Gerald Earle; Phelps, Tommy Joe; Graham, David E; Moon, Ji Won

    2015-01-01

    Bimodal sized elemental copper (Cu) nanoparticles (NPs) were synthesized from inexpensive oxidized copper salts by an extracellular metal-reduction process using anaerobic Thermoanaerobacter sp. X513 bacteria in aqueous solution. The bacteria nucleate NPs outside of the cell, and they control the Cu2+ reduction rate to form uniform crystallites with an average diameter of 1.75 0.46 m after 3-day incubation. To control the size and enhance air stability of Cu NPs, the reaction mixtures were supplemented with nitrilotriacetic acid as a chelator, and the surfactant capping agents oleic acid, oleylamine, ascorbic acid, or L-cysteine. Time-dependent UV-visible absorption measurements and XPS studies indicated well-suspended, bimodal colloidal Cu NPs (70 150 and 5 10 nm) with extended air-stability up to 300 min and stable Cu NP films surfaces with 14% oxidation after 20 days. FTIR spectroscopy suggested that these capping agents were effectively adsorbed on the NP surface providing oxidation resistance in aqueous and dry conditions. Compared to previously reported Cu NP syntheses, this biological process substantially reduced the requirement for hazardous organic solvents and chemical reducing agents, while reducing the levels of Cu oxide impurities in the product. This process was highly reproducible and scalable from 0.01 to 1-L batches.

  15. Size tunable elemental copper nanoparticles: extracellular synthesis by thermoanaerobic bacteria and capping molecules

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Jang, Gyoung Gug; Jacobs, Christopher B.; Gresback, Ryan G.; Ivanov, Ilia N.; Meyer, III, Harry M.; Kidder, Michelle; Joshi, Pooran C.; Jellison, Jr, Gerald Earle; Phelps, Tommy Joe; Graham, David E.; et al

    2014-11-10

    Bimodal sized elemental copper (Cu) nanoparticles (NPs) were synthesized from inexpensive oxidized copper salts by an extracellular metal-reduction process using anaerobic Thermoanaerobacter sp. X513 bacteria in aqueous solution. The bacteria nucleate NPs outside of the cell, and they control the Cu2+ reduction rate to form uniform crystallites with an average diameter of 1.75 0.46 m after 3-day incubation. To control the size and enhance air stability of Cu NPs, the reaction mixtures were supplemented with nitrilotriacetic acid as a chelator, and the surfactant capping agents oleic acid, oleylamine, ascorbic acid, or L-cysteine. Time-dependent UV-visible absorption measurements and XPS studies indicatedmore » well-suspended, bimodal colloidal Cu NPs (70 150 and 5 10 nm) with extended air-stability up to 300 min and stable Cu NP films surfaces with 14% oxidation after 20 days. FTIR spectroscopy suggested that these capping agents were effectively adsorbed on the NP surface providing oxidation resistance in aqueous and dry conditions. Compared to previously reported Cu NP syntheses, this biological process substantially reduced the requirement for hazardous organic solvents and chemical reducing agents, while reducing the levels of Cu oxide impurities in the product. This process was highly reproducible and scalable from 0.01 to 1-L batches.« less

  16. Bacteria increase arid-land soil surface temperature through the production of sunscreens

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Couradeau, Estelle; Karaoz, Ulas; Lim, Hsiao Chien; Nunes da Rocha, Ulisses; Northen, Trent; Brodie, Eoin; Garcia-Pichel, Ferran

    2016-01-20

    Soil surface temperature, an important driver of terrestrial biogeochemical processes, depends strongly on soil albedo, which can be significantly modified by factors such as plant cover. In sparsely vegetated lands, the soil surface can be colonized by photosynthetic microbes that build biocrust communities. Here we use concurrent physical, biochemical and microbiological analyses to show that mature biocrusts can increase surface soil temperature by as much as 10 °C through the accumulation of large quantities of a secondary metabolite, the microbial sunscreen scytonemin, produced by a group of late-successional cyanobacteria. Scytonemin accumulation decreases soil albedo significantly. Such localized warming has apparentmore » and immediate consequences for the soil microbiome, inducing the replacement of thermosensitive bacterial species with more thermotolerant forms. In conclusion, these results reveal that not only vegetation but also microorganisms are a factor in modifying terrestrial albedo, potentially impacting biosphere feedbacks on past and future climate, and call for a direct assessment of such effects at larger scales.« less

  17. TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    America's Oil Dependence Through Innovation | Department of Energy ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's Oil Dependence Through Innovation TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's Oil Dependence Through Innovation June 28, 2011 - 12:00am Addthis WASHINGTON, D.C. - Today, Wednesday, June 29, ARPA-E Director Arun Majumdar will host the Department of Energy's second "Energy Matters" live chat. Dr. Majumdar will

  18. Our Energy Independence - A Live Chat With Dr. Arun Majumdar | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Energy Independence - A Live Chat With Dr. Arun Majumdar Our Energy Independence - A Live Chat With Dr. Arun Majumdar June 29, 2011 - 11:16am Addthis Dr. Arun Majumdar takes your questions about investments we're making to build the clean energy infrastructure of the future. Ginny Simmons Ginny Simmons Former Managing Editor for Energy.gov, Office of Public Affairs How can I participate? Submit a question via E-mail, Facebook or Twitter Watch live at 2 PM [UPDATED with video from

  19. TOMORROW: Secretary Chu To Host Earth Day Live Chat | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Secretary Chu To Host Earth Day Live Chat TOMORROW: Secretary Chu To Host Earth Day Live Chat April 19, 2012 - 12:10pm Addthis WASHINGTON, DC - On Friday, April 20, 2012, U.S. Energy Secretary Steven Chu will host a livechat as part of the Energy Department's Earth Day celebrations to highlight the environmental and economic benefits of the Department's investments in clean energy. The live chat will be streamed on www.energy.gov tomorrow, and will feature questions from both in-person and

  20. Anyone can learn about plasma physics through live-streamed course at PPPL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | Princeton Plasma Physics Lab Anyone can learn about plasma physics through live-streamed course at PPPL By Jeanne Jackson DeVoe June 5, 2015 Tweet Widget Google Plus One Share on Facebook A screenshot of the new SULI website where a course in plasma physics will be live-streamed. A screenshot of the new SULI website where a course in plasma physics will be live-streamed. If you've always wanted to learn about the science behind plasma physics and fusion energy, you can listen to the very

  1. Anyone can learn about plasma physics through live-streamed course at PPPL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | Princeton Plasma Physics Lab Anyone can learn about plasma physics through live-streamed course at PPPL By Jeanne Jackson DeVoe June 5, 2015 Tweet Widget Google Plus One Share on Facebook A screenshot of the new SULI website where a course in plasma physics will be live-streamed. A screenshot of the new SULI website where a course in plasma physics will be live-streamed. If you've always wanted to learn about the science behind plasma physics and fusion energy, you can listen to the very

  2. WATCH LIVE: Secretary Chu at 10:45 am ET | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    WATCH LIVE: Secretary Chu at 10:45 am ET WATCH LIVE: Secretary Chu at 10:45 am ET April 20, 2012 - 9:00am Addthis Amanda Scott Amanda Scott Former Managing Editor, Energy.gov This morning, at 10:45 am ET, we'll be streaming a live chat with Secretary Chu celebrating Earth Day. During the event, Secretary Chu will answer questions from you about the environmental and economic benefits of transitioning to a clean energy economy. If you haven't submitted a question yet, there's still time. We'll be

  3. Environmental Microbiology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Environmental Microbiology Examining the soil beneath our feet Read caption + Many environmental molecular biology studies begin with purified DNA and RNA extracted from the soil. Overview of Research and Highlights Learning about microorganisms-bacteria, algae, and fungi-is essential to understanding how living things interact with their environments. Exploration of environmental microbiology at Los Alamos crosses broad scales of investigation that span from identification of genetic regulatory

  4. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Breakthrough antibacterial approach could resolve serious skin infections August 26, 2014 Los Alamos and partners test ionic liquids to break bacterial biofilm layer and save lives LOS ALAMOS, N.M., Aug. 26, 2014-Like a protective tent over a colony of harmful bacteria, biofilms make the treatment of skin infections especially difficult. Microorganisms protected in a biofilm pose a significant health risk due to their antibiotic resistance and recalcitrance to treatment, and biofilm-protected

  5. Turning Bacteria into Biofuel: Development of an Integrated Microbial Electrocatalytic (MEC) System for Liquid Biofuel Production from CO2

    SciTech Connect (OSTI)

    2010-08-01

    Electrofuels Project: LBNL is improving the natural ability of a common soil bacteria called Ralstonia eutropha to use hydrogen and carbon dioxide for biofuel production. First, LBNL is genetically modifying the bacteria to produce biofuel at higher concentrations. Then, LBNL is using renewable electricity obtained from solar, wind, or wave power to produce high amounts of hydrogen in the presence of the bacteria—increasing the organism’s access to its energy source and improving the efficiency of the biofuel-creation process. Finally, LBNL is tethering electrocatalysts to the bacteria’s surface which will further accelerate the rate at which the organism creates biofuel. LBNL is also developing a chemical method to transform the biofuel that the bacteria produce into ready-to-use jet fuel.

  6. Dr. Kathleen Hogan to Host Live Chat on Industrial Energy Efficiency...

    Broader source: Energy.gov (indexed) [DOE]

    WHAT: Assistant Deputy Secretary of Energy Efficiency Kathleen Hogan to host "Energy Matters" live chat WHEN: Wednesday, November 16th 2:00 PM EST HOW: Watch online at Energy.gov...

  7. LiveChat Wed, 6/29, 2pm: Our Energy Independence | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    How can I participate? Submit a question via E-mail, Facebook or Twitter Watch live on ... Majumdar in advance of the event through email, Twitter or Facebook, by Sending an email ...

  8. Anyone can learn about plasma physics through live-streamed course...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Anyone can learn about plasma physics through live-streamed course at PPPL By Jeanne Jackson DeVoe June 5, 2015 Tweet Widget Google Plus One Share on Facebook A screenshot of the...

  9. Live Webcast on the 2014 Farm Bill's Renewable Energy for America Program

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webcast titled “The 2014 Farm Bill's Renewable Energy for America Program” on May 21, 2014, from 3:00 to 4:00 p.m. Eastern Standard Time.

  10. Live Webinar on the Funding Opportunity for Marine and Hydrokinetic Research and Development University Consortium

    Office of Energy Efficiency and Renewable Energy (EERE)

    On April 24, 2014 from 1:00 - 2:30 PM EDT, the Water Power Program will hold a live webinar to provide information to potential applicants for the Marine and Hydrokinetic (MHK) Research and...

  11. Search for long-lived particles that decay into final states...

    Office of Scientific and Technical Information (OSTI)

    obtained are the most stringent to date. In the specific case of a model in which a Higgs boson in the mass range 125-1000mathrmGeVc2 decays into a pair of long-lived...

  12. 01-08-1998 - VPP Program Saves Lives | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    8-1998 - VPP Program Saves Lives Document Number: NA Effective Date: 01/1998 File (public): PDF icon 01-08-1998_green_alert.pdf Lessons Learned Type: Green

  13. Live Webinar on the Funding Opportunity for Technology Incubator for Wind Energy Innovations

    Broader source: Energy.gov [DOE]

    On April 17, 2014, from 1:00 – 3:00 PM MDT, the Wind Program will hold a live webinar to provide information to potential applicants for the Technology Incubator for Wind Energy Innovations Funding Opportunity.

  14. Live Webinar on the Funding Opportunity for Wind Forecasting Improvement Project in Complex Terrain

    Office of Energy Efficiency and Renewable Energy (EERE)

    On April 21, 2014 from 3:00 to 5:00 PM EST the Wind Program will hold a live webinar to provide information to potential applicants for this Funding Opportunity Announcement. There is no cost to...

  15. We Want to Hear From You: Live Twitter Q&A Tuesday at Noon |...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    for a live Twitter Q&A with Mark Smith from the Energy Department's Clean Cities program. As the vehicle deployment manager for Clean Cities, Smith develops and maintains ...

  16. University Teams to Showcase Affordable, Energy Efficient Living in U.S.

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Solar Decathlon 2011 | Department of Energy to Showcase Affordable, Energy Efficient Living in U.S. Department of Energy Solar Decathlon 2011 University Teams to Showcase Affordable, Energy Efficient Living in U.S. Department of Energy Solar Decathlon 2011 September 22, 2011 - 10:32am Addthis WASHINGTON, DC - Collegiate teams featuring over 4,000 students from around the world have descended on the National Mall's West Potomac Park to showcase the highly energy efficient

  17. Deformation Quantization: Quantum Mechanic Lives and Works in Phase-Space

    Office of Scientific and Technical Information (OSTI)

    (Conference) | SciTech Connect Conference: Deformation Quantization: Quantum Mechanic Lives and Works in Phase-Space Citation Details In-Document Search Title: Deformation Quantization: Quantum Mechanic Lives and Works in Phase-Space Wigner's 1932 quasi-probability Distribution Function in phase-space is a special (Weyl) representation of the density matrix. It has been useful in describing quantum flows in: quantum optics; nuclear physics; decoherence (eg, quantum computing); quantum chaos;

  18. Join Us Live This Friday: Women in Clean Energy Symposium | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Live This Friday: Women in Clean Energy Symposium Join Us Live This Friday: Women in Clean Energy Symposium September 26, 2012 - 2:19pm Addthis NREL researcher Kirsten Alberi works in the luminescence mapping laboratory at the Solar Energy Research Facility (SERF). Here she aligns a laser and sample to map the photoluminescence coming off the sample. | Photo by Dennis Schroeder, NREL. NREL researcher Kirsten Alberi works in the luminescence mapping laboratory at the Solar Energy

  19. Mechanism of biocorrosion of low-alloy steel in a thionic bacteria medium

    SciTech Connect (OSTI)

    Baru, R.L.; Starosvetskaya, Z.O.; Timonin, V.A.

    1986-07-01

    This paper models the process of biocorrosion of Armco iron,low alloy steel 10KhSND (0.69 mass % Ni, 0.7 Cr, 0.55 Cu), and also alloys of Armco iron with chromium, copper, and nickel in concentrations corresponding to the level of their content in steel (of order 0.7%) in a medium of the thionic bacteria of the form Thiobacillus thiooxidans. Biocorrosion of metals inflicts losses on systems of water supply and recirculation in industrial plants, in the oil and gas industry, and in hydraulic structures. Investigations of the surface composition of the steel with the aid of a Cameca scanning electron microanalyzer and analysis of the corrosion products by the atomic absorption method revealed that, in contrast with the model, in the culture liquid the surface of steel 10KhSND and its corrosion products are depleted of nickel.

  20. Activated sludge as substrate for sulfate-reducing bacteria in acid mine drainage treatment

    SciTech Connect (OSTI)

    Al-Ani, W.A.G.; Henry, J.G.; Prasad, D.

    1996-11-01

    Acid mine drainage (AMD), characterized by high concentrations of sulfates and heavy metals and low pH, presents a potential hazard to the environment.Several treatment processes (chemical precipitation, ion exchange, reverse osmosis, electrodialysis and electrolytic recovery) are available, but these are often too expensive. Biological treatment of AMD, mediated by sulfate-reducing bacteria (SRB), seems promising. The objective of this study was to use activated sludge as a carbon source for the SRB and determine the most effective COD/sulfate ratio and hydraulic retention time (HRT) for reducing sulfate. Such information would be useful for the application of the proposed two-stage system to AMD treatment. Since the aim of this study was to obtain sulfate reduction and to avoid methane production, it was decided to operate the digesters initially at low COD/SO{sub 4}{sup 2{minus}} ratios of 1.0, 1.5, and 2.0.

  1. Structure, Function, and Regulation of Antenna Complexes of Green Photosynthetic Bacteria

    SciTech Connect (OSTI)

    Robert E. Blankenship

    2001-04-27

    This project is concerned with the structure and function of the chlorosome antennas found in green photosynthetic bacteria. Chlorosomes are ellipsoidal structures attached to the cytoplasmic side of the inner cell membrane. These antenna complexes provide a very large absorption cross section for light capture. Evidence is overwhelming that the chlorosome represents a very different type of antenna from that found in any other photosynthetic system yet studied. It is now clear that chlorosomes do not contain traditional pigment-proteins, in which the pigments bind to specific sites on proteins. Instead, the chlorosome pigments are organized in vivo into pigment oligomers in which direct pigment-pigment interactions are of dominant importance. Our group has used a multidisciplinary approach to investigate this unique system, including model systems, ultrafast spectroscopy, molecular biology, protein chemistry and X-ray crystallography.

  2. Detecting bacteria and Determining Their Susceptibility to Antibiotics by Stochastic Confinement in Nanoliter Droplets using Plug-Based Microfluidics

    SciTech Connect (OSTI)

    Boedicker, J.; Li, L; Kline, T; Ismagilov, R

    2008-01-01

    This article describes plug-based microfluidic technology that enables rapid detection and drug susceptibility screening of bacteria in samples, including complex biological matrices, without pre-incubation. Unlike conventional bacterial culture and detection methods, which rely on incubation of a sample to increase the concentration of bacteria to detectable levels, this method confines individual bacteria into droplets nanoliters in volume. When single cells are confined into plugs of small volume such that the loading is less than one bacterium per plug, the detection time is proportional to plug volume. Confinement increases cell density and allows released molecules to accumulate around the cell, eliminating the pre-incubation step and reducing the time required to detect the bacteria. We refer to this approach as stochastic confinement. Using the microfluidic hybrid method, this technology was used to determine the antibiogram - or chart of antibiotic sensitivity - of methicillin-resistant Staphylococcus aureus (MRSA) to many antibiotics in a single experiment and to measure the minimal inhibitory concentration (MIC) of the drug cefoxitin (CFX) against this strain. In addition, this technology was used to distinguish between sensitive and resistant strains of S. aureus in samples of human blood plasma. High-throughput microfluidic techniques combined with single-cell measurements also enable multiple tests to be performed simultaneously on a single sample containing bacteria. This technology may provide a method of rapid and effective patient-specific treatment of bacterial infections and could be extended to a variety of applications that require multiple functional tests of bacterial samples on reduced timescales.

  3. Impact of bacteria and bacterial components on osteogenic and adipogenic differentiation of adipose-derived mesenchymal stem cells

    SciTech Connect (OSTI)

    Fiedler, Tomas; Salamon, Achim; Adam, Stefanie; Herzmann, Nicole; Taubenheim, Jan; Peters, Kirsten

    2013-11-01

    Adult mesenchymal stem cells (MSC) are present in several tissues, e.g. bone marrow, heart muscle, brain and subcutaneous adipose tissue. In invasive infections MSC get in contact with bacteria and bacterial components. Not much is known about how bacterial pathogens interact with MSC and how contact to bacteria influences MSC viability and differentiation potential. In this study we investigated the impact of three different wound infection relevant bacteria, Escherichia coli, Staphylococcus aureus, and Streptococcus pyogenes, and the cell wall components lipopolysaccharide (LPS; Gram-negative bacteria) and lipoteichoic acid (LTA; Gram-positive bacteria) on viability, proliferation, and osteogenic as well as adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells (adMSC). We show that all three tested species were able to attach to and internalize into adMSC. The heat-inactivated Gram-negative E. coli as well as LPS were able to induce proliferation and osteogenic differentiation but reduce adipogenic differentiation of adMSC. Conspicuously, the heat-inactivated Gram-positive species showed the same effects on proliferation and adipogenic differentiation, while its cell wall component LTA exhibited no significant impact on adMSC. Therefore, our data demonstrate that osteogenic and adipogenic differentiation of adMSC is influenced in an oppositional fashion by bacterial antigens and that MSC-governed regeneration is not necessarily reduced under infectious conditions. - Highlights: Staphylococcus aureus, Streptococcus pyogenes and Escherichia coli bind to and internalize into adMSC. Heat-inactivated cells of these bacterial species trigger proliferation of adMSC. Heat-inactivated E. coli and LPS induce osteogenic differentiation of adMSC. Heat-inactivated E. coli and LPS reduce adipogenic differentiation of adMSC. LTA does not influence adipogenic or osteogenic differentiation of adMSC.

  4. South Atlantic OCS area living marine resources study. Volume II: an investigation of live bottom habitats north of Cape Fear, North Carolina. Final report

    SciTech Connect (OSTI)

    Not Available

    1981-10-01

    The major objectives of this study were to (1) characterize benthic and nektonic communities associated with representative live bottom habitats on the continental shelf of the South Atlantic Bight, and (2) evaluate factors which might influence these communities, particularly the potential for impact by offshore oil and gas activities. The report describes three study sites at the edge of the continental shelf in a 55-100m depth zone, near Cape Fear, North Carolina.

  5. Bioenergetic studies of coal sulfur oxidation by extremely thermophilic bacteria. Final report, September 15, 1992--August 31, 1997

    SciTech Connect (OSTI)

    Kelly, R.M.; Han, C.J.

    1997-12-31

    Thermoacidophilic microorganisms have been considered for inorganic sulfur removal from coal because of expected improvements in rates of both biotic and abiotic sulfur oxidation reactions with increasing temperature. In this study, the bioenergetic response of the extremely thermoacidophilic archaeon, Metallosphaera sedula, to environmental changes have been examined in relation to its capacity to catalyze pyrite oxidation in coal. Given an appropriate bioenergetic challenge, the metabolic response was to utilize additional amounts of energy sources (i.e., pyrite) to survive. Of particular interest were the consequences of exposing the organism to various forms of stress (chemical, nutritional, thermal, pH) in the presence of coal pyrite. Several approaches to take advantage of stress response to accelerate pyrite oxidation by this organism were examined, including attempts to promote acquired thermal tolerance to extend its functional range, exposure to chemical uncouplers and decouplers, and manipulation of heterotrophic and chemolithotrophic tendencies to optimize biomass concentration and biocatalytic activity. Promising strategies were investigated in a continuous culture system. This study identified environmental conditions that promote better coupling of biotic and abiotic oxidation reactions to improve biosulfurization rates of thermoacidophilic microorganisms.

  6. Structural characterization and comparison of three acyl-carrier-protein synthases from pathogenic bacteria

    SciTech Connect (OSTI)

    Halavaty, Andrei S. [Center for Structural Genomics of Infectious Diseases, (United States); Northwestern University, Chicago, IL 60611 (United States); Kim, Youngchang [Center for Structural Genomics of Infectious Diseases, (United States); Argonne National Laboratory, Argonne, IL 60439 (United States); University of Chicago, Chicago, IL 60637 (United States); Minasov, George; Shuvalova, Ludmilla; Dubrovska, Ievgeniia; Winsor, James [Center for Structural Genomics of Infectious Diseases, (United States); Northwestern University, Chicago, IL 60611 (United States); Zhou, Min [Center for Structural Genomics of Infectious Diseases, (United States); Argonne National Laboratory, Argonne, IL 60439 (United States); University of Chicago, Chicago, IL 60637 (United States); Onopriyenko, Olena; Skarina, Tatiana [Center for Structural Genomics of Infectious Diseases, (United States); University of Toronto, Toronto, Ontario M5G 1L6 (Canada); Papazisi, Leka; Kwon, Keehwan; Peterson, Scott N. [Center for Structural Genomics of Infectious Diseases, (United States); J. Craig Venter Institute, Rockville, MD 20850 (United States); Joachimiak, Andrzej [Center for Structural Genomics of Infectious Diseases, (United States); Argonne National Laboratory, Argonne, IL 60439 (United States); University of Chicago, Chicago, IL 60637 (United States); Savchenko, Alexei [Center for Structural Genomics of Infectious Diseases, (United States); University of Toronto, Toronto, Ontario M5G 1L6 (Canada); Anderson, Wayne F., E-mail: wf-anderson@northwestern.edu [Center for Structural Genomics of Infectious Diseases, (United States); Northwestern University, Chicago, IL 60611 (United States)

    2012-10-01

    The structural characterization of acyl-carrier-protein synthase (AcpS) from three different pathogenic microorganisms is reported. One interesting finding of the present work is a crystal artifact related to the activity of the enzyme, which fortuitously represents an opportunity for a strategy to design a potential inhibitor of a pathogenic AcpS. Some bacterial type II fatty-acid synthesis (FAS II) enzymes have been shown to be important candidates for drug discovery. The scientific and medical quest for new FAS II protein targets continues to stimulate research in this field. One of the possible additional candidates is the acyl-carrier-protein synthase (AcpS) enzyme. Its holo form post-translationally modifies the apo form of an acyl carrier protein (ACP), which assures the constant delivery of thioester intermediates to the discrete enzymes of FAS II. At the Center for Structural Genomics of Infectious Diseases (CSGID), AcpSs from Staphylococcus aureus (AcpS{sub SA}), Vibrio cholerae (AcpS{sub VC}) and Bacillus anthracis (AcpS{sub BA}) have been structurally characterized in their apo, holo and product-bound forms, respectively. The structure of AcpS{sub BA} is emphasized because of the two 3?, 5?-adenosine diphosphate (3?, 5?-ADP) product molecules that are found in each of the three coenzyme A (CoA) binding sites of the trimeric protein. One 3?, 5?-ADP is bound as the 3?, 5?-ADP part of CoA in the known structures of the CoAAcpS and 3?, 5?-ADPAcpS binary complexes. The position of the second 3?, 5?-ADP has never been described before. It is in close proximity to the first 3?, 5?-ADP and the ACP-binding site. The coordination of two ADPs in AcpS{sub BA} may possibly be exploited for the design of AcpS inhibitors that can block binding of both CoA and ACP.

  7. Genotypic and phenotypic characterization of aerosolized bacteria collected from African dust events

    SciTech Connect (OSTI)

    Wilson, Christina A.; Brigmon, Robin L.; Yeager, Chris; Smith, Garriet W.; Polson, Shawn W.

    2013-07-31

    Twenty-one bacteria were isolated and characterized from air samples collected in Africa and the Caribbean by the United States Geological Survey (USGS). Isolates were selected based on preliminary characterization as possible pathogens. Identification of the bacterial isolates was 25 achieved using 16S rRNA gene sequence analysis, fatty acid methyl esters (FAMEs) profiling, the BIOLOG Microlog® System (carbon substrate assay), and repetitive extragenic palindromic (REP)-PCR analysis. The majority of isolates (18/21) were identified as species of the genus Bacillus. Three isolates were classified within the Bacillus cereus senso lato group, which includes Bacillus anthracis, Bacillus thuringiensis, and Bacillus cereus strains. One isolate was identified as a Staphylococcus sp., 30 most closely related to species (i.e Staphylococcus kloosii, Staphylococcus warneri) that are commonly associated with human or animal skin, but can also act as opportunistic pathogen. Another isolate was tentatively identified as Tsukamurella inchonensis, a known respiratory pathogen, and was resistant to the ten antibiotics tested including vancomycin.

  8. Alteration of Iron-Rich Lacustrine Sediments by Dissimilatory Iron-Reducing Bacteria

    SciTech Connect (OSTI)

    Crowe,S.; Roberts, J.; Weisener, C.; Fowle, D.

    2007-01-01

    The reduction of Fe during bacterial anaerobic respiration in sediments and soils not only causes the degradation of organic matter but also results in changes in mineralogy and the redistribution of many nutrients and trace metals. Understanding trace metal patterns in sedimentary rocks and predicting the fate of contaminants in the environment requires a detailed understanding of the mechanisms through which they are redistributed during Fe reduction. In this work, lacustrine sediments from Lake Matano in Indonesia were incubated in a minimal media with the dissimilatory iron reducing (DIR) bacterium Shewanella putrefaciens 200R. These sediments were reductively dissolved at rates slower than pure synthetic goethite despite the presence of an 'easily reducible' component, as defined by selective extractions. DIR of the lacustrine sediments resulted in the substrate-dependent production of abundant quantities of extracellular polymeric substances. Trace elements, including Ni, Co, P, Si, and As, were released from the sediments with progressive Fe reduction while Cr was sequestered. Much of the initial trace metal mobility can be attributed to the rapid reduction of a Mn-rich oxyhydroxide phase. The production of organo-Fe(III) reveals that DIR bacteria can generate significant metal complexation capacity. This work demonstrates that DIR induces the release of many elements associated with Fe-Mn oxyhydroxides, despite secondary mineralization.

  9. [Genetics in methylotrophic bacteria]. Progress report, July 1, 1992--June 30, 1995

    SciTech Connect (OSTI)

    1998-09-01

    The focus of this project has been to identify and characterize mox genes and other methylotrophy genes in both methane- and methanol-utilizing bacteria, and study expression of those genes. In the last three years of support, the project has focused on identifying methylotrophy genes and the regions involved in their expression for comparative purposes, and has begun the process of analyzing the genes involved in transcriptional regulation of the mox system in the strain for which the authors have the most information, M. extorquens AM1. In order to carry out comparative studies of the transcription of methylotrophy genes, they have cloned and characterized genes involved in methanol oxidation (mox genes) from two Type I methanotrophs, Methylobacter marinus A45 (formerly Methylomonas sp A45) and Methylobacter albus BG8 (formerly Methylomonas albus BG8). In both cases, the organization of the genes was found to be identical, and the transcriptional start sites upstream of the mxaF genes were mapped. Other methylotrophy genes have been cloned and characterized from these methanotrophs, including mxaAKL and fdh. The rest of this project has focused on the regulatory network for the mox system in M. extorquens AM1. The authors have sequenced two mox regulatory genes, mxbD and mxbM and they show identify with a specific group of sensor-kinase/response regulator pair systems.

  10. Genotypic and phenotypic characterization of aerosolized bacteria collected from African dust events

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wilson, Christina A.; Brigmon, Robin L.; Yeager, Chris; Smith, Garriet W.; Polson, Shawn W.

    2013-07-31

    Twenty-one bacteria were isolated and characterized from air samples collected in Africa and the Caribbean by the United States Geological Survey (USGS). Isolates were selected based on preliminary characterization as possible pathogens. Identification of the bacterial isolates was 25 achieved using 16S rRNA gene sequence analysis, fatty acid methyl esters (FAMEs) profiling, the BIOLOG Microlog® System (carbon substrate assay), and repetitive extragenic palindromic (REP)-PCR analysis. The majority of isolates (18/21) were identified as species of the genus Bacillus. Three isolates were classified within the Bacillus cereus senso lato group, which includes Bacillus anthracis, Bacillus thuringiensis, and Bacillus cereus strains. Onemore » isolate was identified as a Staphylococcus sp., 30 most closely related to species (i.e Staphylococcus kloosii, Staphylococcus warneri) that are commonly associated with human or animal skin, but can also act as opportunistic pathogen. Another isolate was tentatively identified as Tsukamurella inchonensis, a known respiratory pathogen, and was resistant to the ten antibiotics tested including vancomycin.« less

  11. Table HC1.2.4 Living Space Characteristics by Average Floorspace--Apartments, 2

    U.S. Energy Information Administration (EIA) Indexed Site

    2.4 Living Space Characteristics by Average Floorspace--Apartments, 2005" ,,,"Average Square Feet per Apartment in a --" ," Housing Units (millions)" ,,,"2 to 4 Unit Building",,,"5 or More Unit Building" ,,"Apartments (millions)" "Living Space Characteristics",,,"Total","Heated","Cooled","Total","Heated","Cooled" "Total",111.1,24.5,1090,902,341,872,780,441

  12. LIVE Q&A TODAY: Answering Your Wind Energy Questions | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    LIVE Q&A TODAY: Answering Your Wind Energy Questions LIVE Q&A TODAY: Answering Your Wind Energy Questions August 8, 2013 - 12:03pm Addthis On Thursday, August 8, we hosted a Google+ Hangout on wind energy in America. Watch as our clean energy experts take your questions. Erin R. Pierce Erin R. Pierce Former Digital Communications Specialist, Office of Public Affairs How can I participate? Tweet questions to @ENERGY with the hashtag #AskEnergy. Ask us on Facebook and Google+. Email

  13. Largest Solar Panel Installation at a U.S. University Goes Live |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Largest Solar Panel Installation at a U.S. University Goes Live Largest Solar Panel Installation at a U.S. University Goes Live November 4, 2010 - 6:10pm Addthis Sen. Menendez, Rep. Pascrell, John Lushetsky and other officials at the ribbon cutting. Sen. Menendez, Rep. Pascrell, John Lushetsky and other officials at the ribbon cutting. A couple of weeks ago, I had the opportunity to participate in a ribbon cutting event for the largest solar installation on a United

  14. LiveChat Thurs, 10/20, 2pm ET: Clean Tech Markets | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    LiveChat Thurs, 10/20, 2pm ET: Clean Tech Markets LiveChat Thurs, 10/20, 2pm ET: Clean Tech Markets October 17, 2011 - 11:30am Addthis Liisa O'Neill Liisa O'Neill Former New Media Specialist, Office of Public Affairs How can I participate? Send an email to newmedia@hq.doe.gov; Tweeting your question to @energy with the hashtag #energymatters; or leaving a question for Kauffman at Facebook.com/energygov. On Energy.gov, we've been showcasing a series of stories about innovations from our National

  15. Ask the Directors: Secretary Chu to Host Live Chat on the Energy Innovation

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Hubs | Department of Energy Ask the Directors: Secretary Chu to Host Live Chat on the Energy Innovation Hubs Ask the Directors: Secretary Chu to Host Live Chat on the Energy Innovation Hubs March 5, 2012 - 12:32pm Addthis Secretary Chu is accepting questions on Facebook, Twitter and e-mail for the Energy Innovation Hub directors -- who are working to build better nuclear reactors, obtain fuel from sunlight and design the most energy efficient buildings to date. Michael Hess Michael Hess

  16. Sharing Knowledge for a Low-Carbon Future: Zoellick and Chu in "live"

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    discussion | Department of Energy Sharing Knowledge for a Low-Carbon Future: Zoellick and Chu in "live" discussion Sharing Knowledge for a Low-Carbon Future: Zoellick and Chu in "live" discussion July 13, 2011 - 12:00am Addthis On Wednesday July 13, World Bank President Robert B. Zoellick and US Energy Secretary Steven Chu will discuss how technology and policy can help the world move toward a low-carbon future. Their half-hour discussion at the World Bank's Washington

  17. "Table HC11.2 Living Space Characteristics by Northeast Census Region, 2005"

    U.S. Energy Information Administration (EIA) Indexed Site

    2 Living Space Characteristics by Northeast Census Region, 2005" " Million U.S. Housing Units" ,,"Northeast Census Region" ,"U.S. Housing Units (millions)" ,,,"Census Division" ,,"Total Northeast" "Living Space Characteristics",,,"Middle Atlantic","New England" "Total",111.1,20.6,15.1,5.5 "Floorspace (Square Feet)" "Total Floorspace1" "Fewer than 500",3.2,0.9,0.5,0.4

  18. Photo of the Week: Living Large -- Argonne's First Computer | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Living Large -- Argonne's First Computer Photo of the Week: Living Large -- Argonne's First Computer August 31, 2012 - 9:58am Addthis Before there was Google, or even the Internet, there was the computer -- and the earliest computers were so large that just one could occupy an entire room. AVIDAC was the first digital computer at Argonne National Laboratory, and began operating in 1953. It was built by the Physics Division for $250,000. Pictured here, with AVIDAC, is pioneer Argonne

  19. DOE Live Video Presentation: Smart Grid Development in the U.S. |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Live Video Presentation: Smart Grid Development in the U.S. DOE Live Video Presentation: Smart Grid Development in the U.S. November 9, 2011 - 9:24am Addthis The U.S. Department of Energy's (DOE) Office of Electricity Delivery and Energy Reliability, Office of Energy Efficiency and Renewable Energy, and Brookhaven National Laboratory are conducting a free, online presentation on smart grid development in the U.S, Thursday, Dec. 1, from 11:00 a.m. to 1:00 p.m. EST. This

  20. Five Ways SLAC's X-ray Laser Can Change the Way We Live: 'The First Five

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Years' Points to a Bright Future of High-impact Discovery at LCLS | Department of Energy Five Ways SLAC's X-ray Laser Can Change the Way We Live: 'The First Five Years' Points to a Bright Future of High-impact Discovery at LCLS Five Ways SLAC's X-ray Laser Can Change the Way We Live: 'The First Five Years' Points to a Bright Future of High-impact Discovery at LCLS March 11, 2016 - 11:29am Addthis News release from SLAC National Accelerator Laboratory, March 9, 2016 If you've ever stood in a

  1. #askEnergy: Live Twitter Chat with Home Energy Efficiency Experts |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy askEnergy: Live Twitter Chat with Home Energy Efficiency Experts #askEnergy: Live Twitter Chat with Home Energy Efficiency Experts September 11, 2012 - 10:20am Q&A What questions do you have about improving your home's energy efficiency? Ask Us Addthis The Energy Department's home energy efficiency experts David Lee and Sam Rashkin will be answering your questions on ways to save energy and money at home. | Image courtesy of Sarah Gerrity. The Energy Department's

  2. "Lantern Live" Mobile App Lights Way for Citizens Impacted by Disasters |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy "Lantern Live" Mobile App Lights Way for Citizens Impacted by Disasters "Lantern Live" Mobile App Lights Way for Citizens Impacted by Disasters November 24, 2014 - 5:06pm Addthis (Editor's Note: the following was originally posted on the White House Office of Science and Technology Policy blog on November 21, 2014.) By Brian Forde, Denice Ross and Derek Frempong In the wake of Hurricane Sandy, seemingly simple tasks such as refueling your car were

  3. #askEnergy: Live Twitter Chat with A Solar Expert | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    askEnergy: Live Twitter Chat with A Solar Expert #askEnergy: Live Twitter Chat with A Solar Expert June 5, 2012 - 11:51am Q&A What questions do you have about solar energy? Ask Us Addthis Photo by Dennis Schroeder/NREL. Photo by Dennis Schroeder/NREL. Erin R. Pierce Erin R. Pierce Former Digital Communications Specialist, Office of Public Affairs How can I participate? Join us for a Solar Twitter Chat this Friday, June 8 at 2 pm EDT. To participate, leave a comment on Facebook. Send

  4. DOE Tour of Zero: Options for Community Living by United Way of Long Island

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy Options for Community Living by United Way of Long Island DOE Tour of Zero: Options for Community Living by United Way of Long Island 1 of 14 United Way of Long Island Housing Development Corporation renovated this 1,436-square-foot home in Patchogue, New York, to the performance criteria of the U.S. Department of Energy Zero Energy Ready Home (ZERH) program. 2 of 14 The 1970s-era home had a HERS score of 162 in its pre-renovation condition, as shown here. When

  5. Tribal Summit Live: 9:30-10 AM ET Today and All Day Tomorrow | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Tribal Summit Live: 9:30-10 AM ET Today and All Day Tomorrow Tribal Summit Live: 9:30-10 AM ET Today and All Day Tomorrow May 4, 2011 - 8:00am Addthis Ginny Simmons Ginny Simmons Former Managing Editor for Energy.gov, Office of Public Affairs UPDATE: We've added video from the events. Indian Energy Tribal Summit with American Indian and Alaska Native leaders kicks off this morning in Arlington, Virginia, with welcoming remarks from ARPA-E Director and Senior Advisor to the

  6. WEDNESDAY: Secretary Chu to Host Live Chat on Clean Energy and Innovation |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Secretary Chu to Host Live Chat on Clean Energy and Innovation WEDNESDAY: Secretary Chu to Host Live Chat on Clean Energy and Innovation January 24, 2011 - 12:00am Addthis WASHINGTON, D.C. - U.S. Energy Secretary Steven Chu will host the Department of Energy's inaugural "Energy Matters" online town hall on President Obama's clean energy and innovation agenda as a follow-up to the State of the Union. He will speak to and answer questions from an online and

  7. Live Status

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Help Staff Blogs Request Repository Mailing List Need Help? Out-of-hours Status and Password help Call operations: 1-800-66-NERSC, option 1 or 510-486-6821 Account Support ...

  8. EECBG Success Story: Making the "Best Place to Live" Even Better

    Broader source: Energy.gov [DOE]

    One advantage that Eden Prairie, Minnesota, will have going into next year’s Best Place to Live competition will be the energy efficiency upgrades taking place across the city thanks to $626,000 in Energy Efficiency and Conservation Block Grant (EECBG) funding provided through the American Recovery and Reinvestment Act. Learn more.

  9. Method of freezing living cells and tissues with improved subsequent survival

    DOE Patents [OSTI]

    Senkan, Selim M.; Hirsch, Gerald P.

    1980-01-01

    This invention relates to an improved method for freezing red blood cells, ther living cells, or tissues with improved subsequent survival, wherein constant-volume freezing is utilized that results in significantly improved survival compared with constant-pressure freezing; optimization is attainable through the use of different vessel geometries, cooling baths and warming baths, and sample concentrations.

  10. A novel transcriptional regulator of L-arabinose utilization in human gut bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Chang, Changsoo; Tesar, Christine; Li, Xiaoqing; Kim, Youngchang; Rodionov, Dmitry A.; Joachimiak, Andrzej

    2015-10-04

    We report that carbohydrate metabolism plays a crucial role in the ecophysiology of human gut microbiota. Mechanisms of transcriptional regulation of sugar catabolism in commensal and prevalent human gut bacteria such as Bacteroides thetaiotaomicron remain mostly unknown. By a combination of bioinformatics and experimental approaches, we have identified an NrtR family transcription factor (BT0354 in B. thetaiotaomicron, BtAraR) as a novel regulator controlling the arabinose utilization genes. L-arabinose was confirmed to be a negative effector of BtAraR. We have solved the crystal structures of the apo and L-arabinose-bound BtAraR proteins, as well as the complex of apo-protein with a specificmore » DNA operator. BtAraR forms a homodimer with each subunit comprised of the ligand-binding Nudix hydrolase-like domain and the DNA-binding winged-helix-turn-helix (wHTH) domain. We have identified the residues involved in binding of L-arabinose and recognition of DNA. The majority of these residues are well conserved in the AraR orthologs in Bacteroidetes. In the structure of the BtAraR–DNA complex, we found the unique interaction of arginine intercalating its guanidinum moiety into the base pair stacking of B-DNA. L-arabinose binding induces movement of wHTH domains, resulting in a conformation unsuitable for DNA binding. Furthermore, our analysis facilitates reconstruction of the metabolic and regulatory networks involved in carbohydrate utilization in human gut Bacteroides.« less

  11. A novel transcriptional regulator of L-arabinose utilization in human gut bacteria

    SciTech Connect (OSTI)

    Chang, Changsoo; Tesar, Christine; Li, Xiaoqing; Kim, Youngchang; Rodionov, Dmitry A.; Joachimiak, Andrzej

    2015-10-04

    We report that carbohydrate metabolism plays a crucial role in the ecophysiology of human gut microbiota. Mechanisms of transcriptional regulation of sugar catabolism in commensal and prevalent human gut bacteria such as Bacteroides thetaiotaomicron remain mostly unknown. By a combination of bioinformatics and experimental approaches, we have identified an NrtR family transcription factor (BT0354 in B. thetaiotaomicron, BtAraR) as a novel regulator controlling the arabinose utilization genes. L-arabinose was confirmed to be a negative effector of BtAraR. We have solved the crystal structures of the apo and L-arabinose-bound BtAraR proteins, as well as the complex of apo-protein with a specific DNA operator. BtAraR forms a homodimer with each subunit comprised of the ligand-binding Nudix hydrolase-like domain and the DNA-binding winged-helix-turn-helix (wHTH) domain. We have identified the residues involved in binding of L-arabinose and recognition of DNA. The majority of these residues are well conserved in the AraR orthologs in Bacteroidetes. In the structure of the BtAraR–DNA complex, we found the unique interaction of arginine intercalating its guanidinum moiety into the base pair stacking of B-DNA. L-arabinose binding induces movement of wHTH domains, resulting in a conformation unsuitable for DNA binding. Furthermore, our analysis facilitates reconstruction of the metabolic and regulatory networks involved in carbohydrate utilization in human gut Bacteroides.

  12. Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hill, Steven C.; Williamson, Chatt C.; Doughty, David C.; Pan, Yong-Le; Santarpia, Joshua L.; Hill, Hanna H.

    2015-02-02

    This paper uses a mathematical model of fluorescent biological particles composed of bacteria and/or proteins (mostly as in Hill et al., 2013 [23]) to investigate the size-dependence of the total fluorescence emitted in all directions. The model applies to particles which have negligible reabsorption of fluorescence within the particle. The specific particles modeled here are composed of ovalbumin and of a generic Bacillus. The particles need not be spherical, and in some cases need not be homogeneous. However, the results calculated in this paper are for spherical homogeneous particles. Light absorbing and fluorescing molecules included in the model are aminomore » acids, nucleic acids, and several coenzymes. Here the excitation wavelength is 266 nm. The emission range, 300 to 370 nm, encompasses the fluorescence of tryptophan. The fluorescence cross section (CF) is calculated and compared with one set of published measured values. We investigate power law (Ady) approximations to CF, where d is diameter, and A and y are parameters adjusted to fit the data, and examine how y varies with d and composition, including the fraction as water. The particle's fluorescence efficiency (QF=CF/geometric-cross-section) can be written for homogeneous particles as QabsRF, where Qabs is the absorption efficiency, and RF, the fraction of the absorbed light emitted as fluorescence, is independent of size and shape. When QF is plotted vs. mid or mi(mr-1)d, where m=mr+imi is the complex refractive index, the plots for different fractions of water in the particle tend to overlap.« less

  13. Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria

    SciTech Connect (OSTI)

    Hill, Steven C.; Williamson, Chatt C.; Doughty, David C.; Pan, Yong-Le; Santarpia, Joshua L.; Hill, Hanna H.

    2015-02-02

    This paper uses a mathematical model of fluorescent biological particles composed of bacteria and/or proteins (mostly as in Hill et al., 2013 [23]) to investigate the size-dependence of the total fluorescence emitted in all directions. The model applies to particles which have negligible reabsorption of fluorescence within the particle. The specific particles modeled here are composed of ovalbumin and of a generic Bacillus. The particles need not be spherical, and in some cases need not be homogeneous. However, the results calculated in this paper are for spherical homogeneous particles. Light absorbing and fluorescing molecules included in the model are amino acids, nucleic acids, and several coenzymes. Here the excitation wavelength is 266 nm. The emission range, 300 to 370 nm, encompasses the fluorescence of tryptophan. The fluorescence cross section (CF) is calculated and compared with one set of published measured values. We investigate power law (Ady) approximations to CF, where d is diameter, and A and y are parameters adjusted to fit the data, and examine how y varies with d and composition, including the fraction as water. The particle's fluorescence efficiency (QF=CF/geometric-cross-section) can be written for homogeneous particles as QabsRF, where Qabs is the absorption efficiency, and RF, the fraction of the absorbed light emitted as fluorescence, is independent of size and shape. When QF is plotted vs. mid or mi(mr-1)d, where m=mr+imi is the complex refractive index, the plots for different fractions of water in the particle tend to overlap.

  14. ``Sleeping reactor`` irradiations: Shutdown reactor determination of short-lived activation products

    SciTech Connect (OSTI)

    Jerde, E.A.; Glasgow, D.C.

    1998-09-01

    At the High-Flux Isotope Reactor (HFIR) at the Oak Ridge National Laboratory, the principal irradiation system has a thermal neutron flux ({phi}) of {approximately} 4 {times} 10{sup 14} n/cm{sup 2} {center_dot} s, permitting the detection of elements via irradiation of 60 s or less. Irradiations of 6 or 7 s are acceptable for detection of elements with half-lives of as little as 30 min. However, important elements such as Al, Mg, Ti, and V have half-lives of only a few minutes. At HFIR, these can be determined with irradiation times of {approximately} 6 s, but the requirement of immediate counting leads to increased exposure to the high activity produced by irradiation in the high flux. In addition, pneumatic system timing uncertainties (about {+-} 0.5 s) make irradiations of < 6 s less reliable. Therefore, the determination of these ultra-short-lived species in mixed matrices has not generally been made at HFIR. The authors have found that very short lived activation products can be produced easily during the period after reactor shutdown (SCRAM), but prior to the removal of spent fuel elements. During this 24- to 36-h period (dubbed the ``sleeping reactor``), neutrons are produced in the beryllium reflector by the reaction {sup 9}Be({gamma},n){sup 8}Be, the gamma rays principally originating in the spent fuel. Upon reactor SCRAM, the flux drops to {approximately} 1 {times} 10{sup 10} n/cm{sup 2} {center_dot} s within 1 h. By the time the fuel elements are removed, the flux has dropped to {approximately} 6 {times} 10{sup 8}. Such fluxes are ideal for the determination of short-lived elements such as Al, Ti, Mg, and V. An important feature of the sleeping reactor is a flux that is not constant.

  15. Comparative genomic insights into ecophysiology of neutrophilic, microaerophilic iron oxidizing bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Kato, Shingo; Ohkuma, Moriya; Powell, Deborah H.; Krepski, Sean T.; Oshima, Kenshiro; Hattori, Masahira; Shapiro, Nicole; Woyke, Tanja; Chan, Clara S.

    2015-11-13

    Neutrophilic microaerophilic iron-oxidizing bacteria (FeOB) are thought to play a significant role in cycling of carbon, iron and associated elements in both freshwater and marine iron-rich environments. However, the roles of the neutrophilic microaerophilic FeOB are still poorly understood due largely to the difficulty of cultivation and lack of functional gene markers. Here, we analyze the genomes of two freshwater neutrophilic microaerophilic stalk-forming FeOB, Ferriphaselus amnicola OYT1 and Ferriphaselus strain R-1. Phylogenetic analyses confirm that these are distinct species within Betaproteobacteria; we describe strain R-1 and propose the name F. globulitus. We compare the genomes to those of two freshwatermore » Betaproteobacterial and three marine Zetaproteobacterial FeOB isolates in order to look for mechanisms common to all FeOB, or just stalk-forming FeOB. The OYT1 and R-1 genomes both contain homologs to cyc2, which encodes a protein that has been shown to oxidize Fe in the acidophilic FeOB, Acidithiobacillus ferrooxidans. This c-type cytochrome common to all seven microaerophilic FeOB isolates, strengthening the case for its common utility in the Fe oxidation pathway. In contrast, the OYT1 and R-1 genomes lack mto genes found in other freshwater FeOB. OYT1 and R-1 both have genes that suggest they can oxidize sulfur species. Both have the genes necessary to fix carbon by the Calvin–Benson– Basshom pathway, while only OYT1 has the genes necessary to fix nitrogen. The stalk-forming FeOB share xag genes that may help form the polysaccharide structure of stalks. Both OYT1 and R-1 make a novel biomineralization structure, short rod-shaped Fe oxyhydroxides much smaller than their stalks; these oxides are constantly shed, and may be a vector for C, P, and metal transport to downstream environments. Lastly, our results show that while different FeOB are adapted to particular niches, freshwater and marine FeOB likely share common mechanisms for Fe

  16. Comparative genomic insights into ecophysiology of neutrophilic, microaerophilic iron oxidizing bacteria

    SciTech Connect (OSTI)

    Kato, Shingo; Ohkuma, Moriya; Powell, Deborah H.; Krepski, Sean T.; Oshima, Kenshiro; Hattori, Masahira; Shapiro, Nicole; Woyke, Tanja; Chan, Clara S.

    2015-11-13

    Neutrophilic microaerophilic iron-oxidizing bacteria (FeOB) are thought to play a significant role in cycling of carbon, iron and associated elements in both freshwater and marine iron-rich environments. However, the roles of the neutrophilic microaerophilic FeOB are still poorly understood due largely to the difficulty of cultivation and lack of functional gene markers. Here, we analyze the genomes of two freshwater neutrophilic microaerophilic stalk-forming FeOB, Ferriphaselus amnicola OYT1 and Ferriphaselus strain R-1. Phylogenetic analyses confirm that these are distinct species within Betaproteobacteria; we describe strain R-1 and propose the name F. globulitus. We compare the genomes to those of two freshwater Betaproteobacterial and three marine Zetaproteobacterial FeOB isolates in order to look for mechanisms common to all FeOB, or just stalk-forming FeOB. The OYT1 and R-1 genomes both contain homologs to cyc2, which encodes a protein that has been shown to oxidize Fe in the acidophilic FeOB, Acidithiobacillus ferrooxidans. This c-type cytochrome common to all seven microaerophilic FeOB isolates, strengthening the case for its common utility in the Fe oxidation pathway. In contrast, the OYT1 and R-1 genomes lack mto genes found in other freshwater FeOB. OYT1 and R-1 both have genes that suggest they can oxidize sulfur species. Both have the genes necessary to fix carbon by the Calvin–Benson– Basshom pathway, while only OYT1 has the genes necessary to fix nitrogen. The stalk-forming FeOB share xag genes that may help form the polysaccharide structure of stalks. Both OYT1 and R-1 make a novel biomineralization structure, short rod-shaped Fe oxyhydroxides much smaller than their stalks; these oxides are constantly shed, and may be a vector for C, P, and metal transport to downstream environments. Lastly, our results show that while different FeOB are adapted to particular niches, freshwater and marine FeOB likely share

  17. NNSA team members make a living in nuclear security, make a difference

    National Nuclear Security Administration (NNSA)

    giving back | National Nuclear Security Administration | (NNSA) team members make a living in nuclear security, make a difference giving back Wednesday, April 13, 2016 - 10:38am NNSA Blog NNSA is focused on the mission first, people always, and NNSA's people make a difference, both on and off the clock. During National Volunteer Week, we recognize those across the enterprise who are active, energetic, and engaged in their communities. Every day, members of America's nuclear security

  18. Long-Range Untethered Real-Time Live Gas Main Robotic Inspection System

    SciTech Connect (OSTI)

    Hagen Schempf; Daphne D'Zurko

    2004-10-31

    Under funding from the Department of Energy (DOE) and the Northeast Gas Association (NGA), Carnegie Mellon University (CMU) developed an untethered, wireless remote controlled inspection robot dubbed Explorer. The project entailed the design and prototyping of a wireless self-powered video-inspection robot capable of accessing live 6- and 8-inch diameter cast-iron and steel mains, while traversing turns and Ts and elbows under real-time control with live video feedback to an operator. The design is that of a segmented actively articulated and wheel-leg powered robot design, with fisheye imaging capability and self-powered battery storage and wireless real-time communication link. The prototype was functionally tested in an above ground pipe-network, in order to debug all mechanical, electrical and software subsystems, and develop the necessary deployment and retrieval, as well as obstacle-handling scripts. A pressurized natural gas test-section was used to certify it for operation in natural gas at up to 60 psig. Two subsequent live-main field-trials in both cast-iron and steel pipe, demonstrated its ability to be safely launched, operated and retrieved under real-world conditions. The system's ability to safely and repeatably exidrecover from angled and vertical launchers, traverse multi-thousand foot long pipe-sections, make T and varied-angle elbow-turns while wirelessly sending live video and handling command and control messages, was clearly demonstrated. Video-inspection was clearly shown to be a viable tool to understand the state of this critical buried infrastructure, irrespective of low- (cast-iron) or high-pressure (steel) conditions. This report covers the different aspects of specifications, requirements, design, prototyping, integration and testing and field-trialing of the Explorer platform.

  19. Imaging single cells in a beam of live cyanobacteria with an X-ray laser

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Schot, Gijs, vander

    2015-02-10

    This entry contains ten diffraction patterns, and reconstructions images, of individual living Cyanobium gracile cells, imaged using 517 eV X-rays from the LCLS XFEL. The Hawk software package was used for phasing. The Uppsala aerosol injector was used for sample injection, assuring very low noise levels. The cells come from various stages of the cell cycle, and were imaged in random orientations.

  20. In situ Observation of Sulfur in Living Mammalian Cells: Uptake of Taurine

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    into MDCK Cells In situ Observation of Sulfur in Living Mammalian Cells: Uptake of Taurine into MDCK Cells Sulfur is essential for life. It plays important roles in the amino acids methionine and cysteine, and has a structural function in disulfide bonds. As a component of iron-sulfur clusters it takes part in electron and sulfur transfer reactions.1 Glutathione, a sulfur-containing tripeptide, is an important part of biological antioxidant systems.2 Another example for the biological

  1. Ultrafast nanolaser device for detecting cancer in a single live cell.

    SciTech Connect (OSTI)

    Gourley, Paul Lee; McDonald, Anthony Eugene

    2007-11-01

    Emerging BioMicroNanotechnologies have the potential to provide accurate, realtime, high throughput screening of live tumor cells without invasive chemical reagents when coupled with ultrafast laser methods. These optically based methods are critical to advancing early detection, diagnosis, and treatment of disease. The first year goals of this project are to develop a laser-based imaging system integrated with an in- vitro, live-cell, micro-culture to study mammalian cells under controlled conditions. In the second year, the system will be used to elucidate the morphology and distribution of mitochondria in the normal cell respiration state and in the disease state for normal and disease states of the cell. In this work we designed and built an in-vitro, live-cell culture microsystem to study mammalian cells under controlled conditions of pH, temp, CO2, Ox, humidity, on engineered material surfaces. We demonstrated viability of cell culture in the microsystem by showing that cells retain healthy growth rates, exhibit normal morphology, and grow to confluence without blebbing or other adverse influences of the material surfaces. We also demonstrated the feasibility of integrating the culture microsystem with laser-imaging and performed nanolaser flow spectrocytometry to carry out analysis of the cells isolated mitochondria.

  2. Tracking chemical changes in a live cell: Biomedical applications of SR-FTIR spectromicroscopy

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Holman, Hoi-Ying N.; Martin, Michael C.; McKinney, Wayne R.

    2003-01-01

    Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectromicroscopy is a newly emerging bioanalytical and imaging tool. This unique technique provides mid-infrared (IR) spectra, hence chemical information, with high signal-to-noise at spatial resolutions as fine as 3 to 10 microns. Thus it enables researchers to locate, identify, and track specific chemical events within an individual living mammalian cell. Mid-IR photons are too low in energy (0.05-0.5 eV) to either break bonds or to cause ionization. In this review, we show that the synchrotron IR beam has no detectable effects on the short- and long-term viability, reproductive integrity, cell-cycle progression, and mitochondrial metabolismmore » in living human cells, and produces only minimal sample heating (<0.5°C). We will then present several examples demonstrating the application potentials of SR-FTIR spectromicroscopy in biomedical research. These will include monitoring living cells progressing through the cell cycle, including death, and cells reacting to dilute concentrations of toxins.« less

  3. The Effect of Bicarbonate on the Microbial Dissolution of Autunite Mineral in the Presence of Gram-Positive Bacteria

    SciTech Connect (OSTI)

    Sepulveda-Medina, Paola; Katsenovich, Yelena; Wellman, Dawn M.; Lagos, Leonel

    2015-06-01

    Bacteria are key players in the processes that govern fate and transport of contaminants. The uranium release from Na and Ca-autunite by Arthrobacter oxydans strain G968 was evaluated in the presence of bicarbonate ions. This bacterium was previously isolated from Hanford Site soil and in earlier prescreening tests demonstrated low tolerance to U(VI) toxicity compared to other A.oxydans isolates. Experiments were conducted using glass serum bottles as mixed bioreactors and sterile 6-well cell culture plates with inserts separating bacteria cells from mineral solids. Reactors containing phosphorus-limiting media were amended with bicarbonate ranging between 0-10 mM and metaautunite solids to provide a U(VI) concentration of 4.4 mmol/L. Results showed that in the presence of bicarbonate, A.oxydans G968 was able to enhance the release of U(VI) from Na and Ca autunite at the same capacity as other A.oxydans isolates with relatively high tolerance to U(VI). The effect of bacterial strains on autunite dissolution decreases as the concentration of bicarbonate increases. The results illustrate that direct interaction between the bacteria and the mineral is not necessary to result in U (VI) biorelease from autunite. The formation of secondary calcium-phosphate mineral phases on the surface of the mineral during the dissolution can ultimately reduce the natural autunite mineral contact area, which bacterial cells can access. This thereby reduces the concentration of uranium released into the solution. This study provides a better understanding of the interactions between meta-autunite and microbes in conditions mimicking arid and semiarid subsurface environments of western U.S.

  4. Use of bromodeoxyuridine immunocapture to identify psychrotolerant phenanthrene-degrading bacteria in phenanthrene-enriched polluted Baltic Sea sediments

    SciTech Connect (OSTI)

    Edlund, A.; Jansson, J.

    2008-05-01

    The aim of this study was to enrich and identify psychrotolerant phenanthrenedegrading bacteria from polluted Baltic Sea sediments. Polyaromatic hydrocarbon (PAH)-contaminated sediments were spiked with phenanthrene and incubated for 2 months in the presence of bromodeoxyuridine that is incorporated into the DNA of replicating cells. The bromodeoxyuridine-incorporated DNA was extracted by immunocapture and analyzed by terminal-restriction fragment length polymorphism and 16S rRNA gene cloning and sequencing to identify bacterial populations that were growing. In addition, degradation genes were quantified in the bromodeoxyuridine-incorporated DNA by real-time PCR. Phenanthrene concentrations decreased after 2 months of incubation in the phenanthrene-enriched sediments and this reduction correlated to increases in copy numbers of xylE and phnAc dioxygenase genes. Representatives of Exiguobacterium, Schewanella,Methylomonas, Pseudomonas, Bacteroides and an uncultured Deltaproteobacterium and a Gammaproteobacterium dominated the growing community in the phenanthrene spiked sediments. Isolates that were closely related to three of these bacteria (two pseudomonads and an Exiguobacterium sp.) could reduce phenanthrene concentrations in pure cultures and they all harbored phnAc dioxygenase genes. These results confirm that this combination of culture-based and molecular approaches was useful for identification of actively growing bacterial species with a high potential for phenanthrene degradation.

  5. Mechanical properties of interacting lipopolysaccharide membranes from bacteria mutants studied by specular and off-specular neutron scattering

    SciTech Connect (OSTI)

    Schneck, Emanuel; Tanaka, Motomu; Oliveira, Rafael G.; Rehfeldt, Florian; Deme, Bruno; Brandenburg, Klaus; Seydel, Ulrich

    2009-10-15

    Specular and off-specular neutron scattering are used to study the influence of molecular chemistry (mutation) on the intermembrane interactions and mechanical properties of the outer membrane of Gram-negative bacteria consisting of lipopolysaccharides (LPSs). For this purpose, solid-supported multilayers of mutant LPS membranes are deposited on silicon wafers and hydrated either at defined humidity or in bulk buffers. The planar sample geometry allows to identify out-of-plane and in-plane scattering vector components. The measured two-dimensional reciprocal space maps are simulated with membrane displacement correlation functions determined by two mechanical parameters (vertical compression modulus and bending rigidity) and an effective cutoff radius for the membrane fluctuation wavelength. Experiments at controlled humidity enable one to examine the influence of the disjoining pressure on the saccharide-mediated intermembrane interactions, while experiments in bulk buffers (i.e., in the absence of an external osmotic stress) reveal the effect of divalent cations on LPS membranes, highlighting the role of divalent cations in the survival mechanism of bacteria in the presence of antimicrobial molecules.

  6. Developing new optical imaging techniques for single particle and molecule tracking in live cells

    SciTech Connect (OSTI)

    Sun, Wei

    2010-12-15

    Differential interference contrast (DIC) microscopy is a far-field as well as wide-field optical imaging technique. Since it is non-invasive and requires no sample staining, DIC microscopy is suitable for tracking the motion of target molecules in live cells without interfering their functions. In addition, high numerical aperture objectives and condensers can be used in DIC microscopy. The depth of focus of DIC is shallow, which gives DIC much better optical sectioning ability than those of phase contrast and dark field microscopies. In this work, DIC was utilized to study dynamic biological processes including endocytosis and intracellular transport in live cells. The suitability of DIC microscopy for single particle tracking in live cells was first demonstrated by using DIC to monitor the entire endocytosis process of one mesoporous silica nanoparticle (MSN) into a live mammalian cell. By taking advantage of the optical sectioning ability of DIC, we recorded the depth profile of the MSN during the endocytosis process. The shape change around the nanoparticle due to the formation of a vesicle was also captured. DIC microscopy was further modified that the sample can be illuminated and imaged at two wavelengths simultaneously. By using the new technique, noble metal nanoparticles with different shapes and sizes were selectively imaged. Among all the examined metal nanoparticles, gold nanoparticles in rod shapes were found to be especially useful. Due to their anisotropic optical properties, gold nanorods showed as diffraction-limited spots with disproportionate bright and dark parts that are strongly dependent on their orientation in the 3D space. Gold nanorods were developed as orientation nanoprobes and were successfully used to report the self-rotation of gliding microtubules on kinesin coated substrates. Gold nanorods were further used to study the rotational motions of cargoes during the endocytosis and intracellular transport processes in live mammalian

  7. Cadmium, mercury, and lead in kidney cortex of living kidney donors: Impact of different exposure sources,

    SciTech Connect (OSTI)

    Barregard, Lars; Fabricius-Lagging, Elisabeth; Lundh, Thomas; Moelne, Johan; Wallin, Maria; Olausson, Michael; Modigh, Cecilia; Sallsten, Gerd

    2010-01-15

    Background: Most current knowledge on kidney concentrations of nephrotoxic metals like cadmium (Cd), mercury (Hg), or lead (Pb) comes from autopsy studies. Assessment of metal concentrations in kidney biopsies from living subjects can be combined with information about exposure sources like smoking, diet, and occupation supplied by the biopsied subjects themselves. Objectives: To determine kidney concentrations of Cd, Hg, and Pb in living kidney donors, and assess associations with common exposure sources and background factors. Methods: Metal concentrations were determined in 109 living kidney donors aged 24-70 years (median 51), using inductively coupled plasma-mass spectrometry (Cd and Pb) and cold vapor atomic fluorescence spectrometry (Hg). Smoking habits, occupation, dental amalgam, fish consumption, and iron stores were evaluated. Results: The median kidney concentrations were 12.9 {mu}g/g (wet weight) for cadmium, 0.21 {mu}g/g for mercury, and 0.08 {mu}g/g for lead. Kidney Cd increased by 3.9 {mu}g/g for a 10 year increase in age, and by 3.7 {mu}g/g for an extra 10 pack-years of smoking. Levels in non-smokers were similar to those found in the 1970s. Low iron stores (low serum ferritin) in women increased kidney Cd by 4.5 {mu}g/g. Kidney Hg increased by 6% for every additional amalgam surface, but was not associated with fish consumption. Lead was unaffected by the background factors surveyed. Conclusions: In Sweden, kidney Cd levels have decreased due to less smoking, while the impact of diet seems unchanged. Dental amalgam is the main determinant of kidney Hg. Kidney Pb levels are very low due to decreased exposure.

  8. Table HC6.2 Living Space Characteristics by Number of Household Members, 2005

    U.S. Energy Information Administration (EIA) Indexed Site

    2 Living Space Characteristics by Number of Household Members, 2005 Total...................................................................... 111.1 30.0 34.8 18.4 15.9 12.0 Floorspace (Square Feet) Total Floorspace 1 Fewer than 500............................................... 3.2 1.7 0.8 0.4 0.3 Q 500 to 999....................................................... 23.8 10.2 6.4 3.4 2.3 1.5 1,000 to 1,499................................................. 20.8 5.5 6.3 3.0 3.3 2.6 1,500 to

  9. System, device, and methods for real-time screening of live cells, biomarkers, and chemical signatures

    DOE Patents [OSTI]

    Sundaram, S Kamakshi [Richland, WA; Riley, Brian J [West Richland, WA; Weber, Thomas J [Richland, WA; Sacksteder, Colette A [West Richland, WA; Addleman, R Shane [Benton City, WA

    2011-06-07

    An ATR-FTIR device and system are described that defect live-cell responses to stimuli and perturbations in real-time. The system and device can monitor perturbations resulting from exposures to various physical, chemical, and biological materials in real-time, as well as those sustained over a long period of time, including those associated with stimuli having unknown modes-of-action (e.g. nanoparticles). The device and system can also be used to identify specific chemical species or substances that profile cellular responses to these perturbations.

  10. Electric and magnetic field exposures for people living near a 735-Kilovolt power line

    SciTech Connect (OSTI)

    Levallois, P.; Gauvin, D.; St. Laurent, J.

    1995-09-01

    The purpose of this study was to assess the effect of a 735-kV transmission line on the electric and magnetic field exposures of people living at the edge of the line`s right of way. Exposure of 18 adults, mostly white-collar workers, living in different bungalows located 190-240 feet from the line (exposed subjects) was compared to that of 17 adults living in similar residences far away from any transmission line. Each subject carried a Positron meter for 24 hr during 1 workday, which measured 60-Hz electric and magnetic fields every minute. All measurements were carried out in parallel for exposed and unexposed subjects during the same weeks between September and December. During measurements the average loading on the line varied between 600 and 1100 A. The average magnetic field intensity while at home was 4.4 times higher among exposed subjects than unexposed (7.1 versus 1.6 Mg, p=0.0001) and 6.2 times higher when considering only the sleeping period (6.8 versus 1.1 mG, p=0.0001). Based on the 24-hr measurement, average magnetic field exposure was three times higher among the exposed was positively correlated with the loading on the line (r=0.8, p+0.001). Percentage of time above a magnetic field threshold F(2 mG or 7.8 mG) was a good indicator to distinguish the two types of exposure. Percentage of time above 20 V/m was significantly different, but percentage of time above 78 V/m was rare and comparable for the two groups. Variability of exposure was very low. This study demonstrates that a 735-kV line contributes significantly to residential 60-Hz magnetic field exposure and, to a lesser extent, electric fields for people living at the edge of the right way. Because of the limited size of our sample, caution is recommended before generalizing these results. Nevertheless, due to the uncertainty on the risks associated with such an unusual high residential exposure, research is needed on its possible effects. 30 refs., 1 fig., 7 tabs.

  11. Three dimensional time-gated tracking of non-blinking quantum dots in live cells

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    DeVore, Matthew S.; Werner, James H.; Goodwin, Peter M.; Keller, Aaron M.; Hollingsworth, Jennifer A.; Wilson, Bridget S.; Cleyrat, Cedric; Lidke, Diane S.; Ghosh, Yagnaseni; Stewart, Michael H.; et al

    2015-03-12

    Single particle tracking has provided a wealth of information about biophysical processes such as motor protein transport and diffusion in cell membranes. However, motion out of the plane of the microscope or blinking of the fluorescent probe used as a label generally limits observation times to several seconds. Here, we overcome these limitations by using novel non-blinking quantum dots as probes and employing a custom 3D tracking microscope to actively follow motion in three dimensions (3D) in live cells. As a result, signal-to-noise is improved in the cellular milieu through the use of pulsed excitation and time-gated detection.

  12. Installation of 2 7/8-in. coiled-tubing tailpipes in live gas wells

    SciTech Connect (OSTI)

    Campbell, J.A.; Bayes, K.P.

    1994-05-01

    This paper describes a technique for installing 2 7/8-in. coiled tubing as tailpipe extensions below existing production packers in live gas wells. It also covers the use of coiled tubing as a way to complete wells. Large savings in rig time and deferred production have been realized with this technique. Fluid losses to the formation do not occur, and no expensive rig time is needed to kill or clean up the wells, as required for conventional workovers below existing production packers. This technique is particularly applicable in depleted reservoirs that could be impaired by traditional workover methods.

  13. Quantum non-locality in a two-slit interferometer for short-lived particles

    SciTech Connect (OSTI)

    Klein, Spencer R.; Nystrand, Joakim

    2001-12-01

    We describe a new test of quantum nonlocality, using an interferometer for short-lived particles. The separation is large compared with the particle lifetimes. This interferometer is realized by vector meson production in distant heavy ion collisions. The mesons decay before waves from the two sources (ions) can overlap, so interference is only possible among the decay products. The post-decay wave function must retain amplitudes for all possible decays. The decay products are spatially separated, necessitating a non-local wave function. The interference is measurable by summing the product momenta. Alternately, the products positions could be observed, allowing new tests of the EPR paradox.

  14. COLLOQUIUM: Are You Living In A Simulation? | Princeton Plasma Physics Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    February 1, 2017, 4:15pm to 5:30pm Colloquia MBG Auditorium, PPPL (284 cap.) COLLOQUIUM: Are You Living In A Simulation? Dr. Silas Beane University of Washington Seattle Colloquium Committee: The Princeton Plasma Physics Laboratory 2016-2017 Colloquium Committee is comprised of the following people. Please feel free to contact them by e-mail regarding any possible speakers or topics for future colloquia. Carol Ann Austin, caustin@pppl.gov Soha Aslam, saslam@pppl.gov Ilya Dodin, idodin@pppl.gov

  15. Acting Biomass Program Manager Dr. Valerie Reed to Host Live Twitter Q&A on Advanced Biofuels

    Broader source: Energy.gov [DOE]

    Washington, D.C. – On Friday, December 16th, the Energy Department (@energy) will be hosting a live Twitter Q&A on biofuels with Dr. Valerie Reed, Acting Manager of the Biomass Program.

  16. Theory of long-lived nuclear spin states in methyl groups and quantum-rotor induced polarisation

    SciTech Connect (OSTI)

    Dumez, Jean-Nicolas; Håkansson, Pär; Mamone, Salvatore; Meier, Benno; Stevanato, Gabriele; Hill-Cousins, Joseph T.; Roy, Soumya Singha; Brown, Richard C. D.; Pileio, Giuseppe; Levitt, Malcolm H.

    2015-01-28

    Long-lived nuclear spin states have a relaxation time much longer than the longitudinal relaxation time T{sub 1}. Long-lived states extend significantly the time scales that may be probed with magnetic resonance, with possible applications to transport and binding studies, and to hyperpolarised imaging. Rapidly rotating methyl groups in solution may support a long-lived state, consisting of a population imbalance between states of different spin exchange symmetries. Here, we expand the formalism for describing the behaviour of long-lived nuclear spin states in methyl groups, with special attention to the hyperpolarisation effects observed in {sup 13}CH{sub 3} groups upon rapidly converting a material with low-barrier methyl rotation from the cryogenic solid state to a room-temperature solution [M. Icker and S. Berger, J. Magn. Reson. 219, 1 (2012)]. We analyse the relaxation properties of methyl long-lived states using semi-classical relaxation theory. Numerical simulations are supplemented with a spherical-tensor analysis, which captures the essential properties of methyl long-lived states.

  17. Short-lived pollutants in the Arctic: their climate impact and possible mitigation strategies

    SciTech Connect (OSTI)

    Menon, Surabi; Quinn, P.K.; Bates, T.S.; Baum, E.; Doubleday, N.; Fiore, A.M.; Flanner, M.; Fridlind, A.; Garrett, T.J.; Koch, D.; Menon, S.; Shindell, D.; Stohl, A.; Warren, S.G.

    2007-09-24

    Several short-lived pollutants known to impact Arctic climate may be contributing to the accelerated rates of warming observed in this region relative to the global annually averaged temperature increase. Here, we present a summary of the short-lived pollutants that impact Arctic climate including methane, tropospheric ozone, and tropospheric aerosols. For each pollutant, we provide a description of the major sources and the mechanism of forcing. We also provide the first seasonally averaged forcing and corresponding temperature response estimates focused specifically on the Arctic. The calculations indicate that the forcings due to black carbon, methane, and tropospheric ozone lead to a positive surface temperature response indicating the need to reduce emissions of these species within and outside the Arctic. Additional aerosol species may also lead to surface warming if the aerosol is coincident with thin, low lying clouds. We suggest strategies for reducing the warming based on current knowledge and discuss directions for future research to address the large remaining uncertainties.

  18. Method for detection of long-lived radioisotopes in small biochemical samples

    DOE Patents [OSTI]

    Turteltaub, K.W.; Vogel, J.S.; Felton, J.S.; Gledhill, B.L.; Davis, J.C.

    1994-11-22

    Disclosed is a method for detection of long-lived radioisotopes in small biochemical samples, comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering the chemical specie to the biologist host in doses sufficiently low to avoid significant overt damage to the biological system, d. allowing a period of time to elapse sufficient for dissemination and interaction of the chemical specie with the host throughout the biological system of the host, e. isolating a reacted fraction of the biological substance from the host in a manner sufficient to avoid contamination of the substance from extraneous sources, f. converting the fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in the material by means of direct isotopic counting. 5 figs.

  19. Method for detection of long-lived radioisotopes in small biochemical samples

    DOE Patents [OSTI]

    Turteltaub, Kenneth W.; Vogel, John S.; Felton, James S.; Gledhill, Barton L.; Davis, Jay C.

    1994-01-01

    Disclosed is a method for detection of long-lived radioisotopes in small bio-chemical samples, comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering said chemical specie to said biologist host in doses sufficiently low to avoid significant overt damage to the biological system thereof, d. allowing a period of time to elapse sufficient for dissemination and interaction of said chemical specie with said host throughout said biological system of said host, e. isolating a reacted fraction of the biological substance from said host in a manner sufficient to avoid contamination of said substance from extraneous sources, f. converting said fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in said material by means of direct isotopic counting.

  20. Proteolysis in hyperthermophilic microorganisms (Journal Article...

    Office of Scientific and Technical Information (OSTI)

    Authors: Ward, Donald E. ; Shockley, Keith R. ; Chang, Lara S. ; Levy, Ryan D. ; Michel, Joshua K. ; Conners, Shannon B. ; Kelly, Robert M. Publication Date: 2002-01-01 OSTI ...

  1. Microorganism genomics, compositions and methods related thereto

    DOE Patents [OSTI]

    Handelsman, Jo; Goodman, Robert M.; Rondon, Michelle R.

    2001-01-01

    The present invention provides methods and compositions for accessing, in a generally unbaised manner, a diverse genetic pool for genes involved in biosynthetic pathways. The invention also provides compounds which can be identified by cloning biosynthetic pathways.

  2. Manila clams from Hg polluted sediments of Marano and Grado lagoons (Italy) harbor detoxifying Hg resistant bacteria in soft tissues

    SciTech Connect (OSTI)

    Baldi, Franco; Gallo, Michele; Marchetto, Davide; Faleri, Claudia; Maida, Isabel; Fani, Renato

    2013-08-15

    A mechanism of mercury detoxification has been suggested by a previous study on Hg bioaccumulation in Manila clams (Ruditapes philippinarum) in the polluted Marano and Grado lagoons and in this study we demonstrate that this event could be partly related to the detoxifying activities of Hg-resistant bacteria (MRB) harbored in clam soft tissues. Therefore, natural clams were collected in six stations during two different periods (winter and spring) from Marano and Grado Lagoons. Siphons, gills and hepatopancreas from acclimatized clams were sterile dissected to isolate MRB. These anatomical parts were glass homogenized or used for whole, and they were lying on a solid medium containing 5 mg l{sup −1} HgCl{sub 2} and incubated at 30 °C. A total of fourteen bacterial strains were isolated and were identified by 16S rDNA sequencing and analysis, revealing that strains were representative of eight bacterial genera, four of which were Gram-positive (Enterococcus, Bacillus, Jeotgalicoccus and Staphylococcus) and other four were Gram-negative (Stenotrophomonas, Vibrio, Raoultella and Enterobacter). Plasmids and merA genes were found and their sequences determined. Fluorescence in situ hybridization (FISH) technique shows the presence of Firmicutes, Actinobacteria and Gammaproteobacteria by using different molecular probes in siphon and gills. Bacterial clumps inside clam flesh were observed and even a Gram-negative endosymbiont was disclosed by transmission electronic microscope inside clam cells. Bacteria harbored in cavities of soft tissue have mercury detoxifying activity. This feature was confirmed by the determination of mercuric reductase in glass-homogenized siphons and gills. -- Highlights: ► We isolated Gram-positive and Gram-negative Hg resistant strains from soft tissues of Ruditapes philippinarum. ► We identify 14 mercury resistant strains by 16S rRNA gene sequences. ► Bacteria in siphon and gill tissues of clams were observed by TEM and identified

  3. Mechanism of reactivation of the UV-inactivated cells of Escherichia coli by cell extracts of propionic acid bacteria

    SciTech Connect (OSTI)

    Vorob`eva, L.I.; Khodzhaev, E.Y.; Ponomareva, G.M.

    1995-09-01

    Two mechanisms of reactivation of UV-inactivated Escherichia coli cells - photoreactivation (PhR) and reactivation by the dialyzate of cell extract of propionic acid bacteria - are shown to be different but not completely additive. PhR displays an insignificant negative effect on the reactivaton by active substances (peptides) of the dialyzate, whereas reactivation by dialyzate inhibits PhR. The maximal reactivation can be attained under complete PhR followed by the protective action of dialyzate. The dialyzate protects UV-irradiated E. coli cells with PolA, UvrA, and RecA mutations and Salmonella typhimurium TA 100 (UvrB) cells, and also exerts an antimutagenic effect on S. typhimurium TA 100. Protection by dialyzate is suggested to be due to restoration of the cell division mechanism damaged by UV irradiation. 14 refs., 3 figs., 5 tabs.

  4. Living in a Materials World: Materials Science Engineering Professional Development for K-12 Educators

    SciTech Connect (OSTI)

    Anne Seifert; Louis Nadelson

    2011-06-01

    Advances in materials science are fundamental to technological developments and have broad societal impacs. For example, a cellular phone is composed of a polymer case, liquid crystal displays, LEDs, silicon chips, Ni-Cd batteries, resistors, capacitors, speakers, microphones all of which have required advances in materials science to be compacted into a phone which is typically smaller than a deck of cards. Like many technological developments, cellular phones have become a ubiquitous part of society, and yet most people know little about the materials science associated with their manufacture. The probable condition of constrained knowledge of materials science was the motivation for developing and offering a 20 hour fourday course called 'Living in a Materials World.' In addition, materials science provides a connection between our every day experiences and the work of scientists and engineers. The course was offered as part of a larger K-12 teacher professional development project and was a component of a week-long summer institute designed specifically for upper elementary and middle school teachers which included 20 hour content strands, and 12 hours of plenary sessions, planning, and collaborative sharing. The focus of the institute was on enhancing teacher content knowledge in STEM, their capacity for teaching using inquiry, their comfort and positive attitudes toward teaching STEM, their knowledge of how people learn, and strategies for integrating STEM throughout the curriculum. In addition to the summer institute the participating teachers were provided with a kit of about $300 worth of materials and equipment to use to implement the content they learned in their classrooms. As part of this professional development project the participants were required to design and implement 5 lesson plans with their students this fall and report on the results, as part of the continuing education course associated with the project. 'Living in a Materials World' was

  5. Testing, Modeling, and Monitoring to Enable Simpler, Cheaper, Longer-Lived Surface Caps

    SciTech Connect (OSTI)

    Piet, Steven James; Breckenridge, Robert Paul; Burns, Douglas Edward

    2003-02-01

    Society has and will continue to generate hazardous wastes whose risks must be managed. For exceptionally toxic, long-lived, and feared waste, the solution is deep burial, e.g., deep geological disposal at Yucca Mtn. For some waste, recycle or destruction/treatment is possible. The alternative for other wastes is storage at or near the ground level (in someones back yard); most of these storage sites include a surface barrier (cap) to prevent downward water migration. Some of the hazards will persist indefinitely. As society and regulators have demanded additional proof that caps are robust against more threats and for longer time periods, the caps have become increasingly complex and expensive. As in other industries, increased complexity will eventually increase the difficulty in estimating performance, in monitoring system/component performance, and in repairing or upgrading barriers as risks are managed. An approach leading to simpler, less expensive, longer-lived, more manageable caps is needed. Our project, which started in April 2002, aims to catalyze a Barrier Improvement Cycle (iterative learning and application) and thus enable Remediation System Performance Management (doing the right maintenance neither too early nor too late). The knowledge gained and the capabilities built will help verify the adequacy of past remedial decisions, improve barrier management, and enable improved solutions for future decisions. We believe it will be possible to develop simpler, longer-lived, less expensive caps that are easier to monitor, manage, and repair. The project is planned to: a) improve the knowledge of degradation mechanisms in times shorter than service life; b) improve modeling of barrier degradation dynamics; c) develop sensor systems to identify early degradation; and d) provide a better basis for developing and testing of new barrier systems. This project combines selected exploratory studies (benchtop and field scale), coupled effects accelerated aging

  6. Testing, Modeling, and Monitoring to Enable Simpler, Cheaper, Longer-lived Surface Caps

    SciTech Connect (OSTI)

    Piet, S. J.; Breckenridge, R. P.; Burns, D. E.

    2003-02-25

    Society has and will continue to generate hazardous wastes whose risks must be managed. For exceptionally toxic, long-lived, and feared waste, the solution is deep burial, e.g., deep geological disposal at Yucca Mtn. For some waste, recycle or destruction/treatment is possible. The alternative for other wastes is storage at or near the ground level (in someone's back yard); most of these storage sites include a surface barrier (cap) to prevent downward water migration. Some of the hazards will persist indefinitely. As society and regulators have demanded additional proof that caps are robust against more threats and for longer time periods, the caps have become increasingly complex and expensive. As in other industries, increased complexity will eventually increase the difficulty in estimating performance, in monitoring system/component performance, and in repairing or upgrading barriers as risks are managed. An approach leading to simpler, less expensive, longer-lived, more manageable caps is needed. Our project, which started in April 2002, aims to catalyze a Barrier Improvement Cycle (iterative learning and application) and thus enable Remediation System Performance Management (doing the right maintenance neither too early nor too late). The knowledge gained and the capabilities built will help verify the adequacy of past remedial decisions, improve barrier management, and enable improved solutions for future decisions. We believe it will be possible to develop simpler, longer-lived, less expensive caps that are easier to monitor, manage, and repair. The project is planned to: (a) improve the knowledge of degradation mechanisms in times shorter than service life; (b) improve modeling of barrier degradation dynamics; (c) develop sensor systems to identify early degradation; and (d) provide a better basis for developing and testing of new barrier systems. This project combines selected exploratory studies (benchtop and field scale), coupled effects accelerated

  7. Copper extraction from coarsely ground printed circuit boards using moderate thermophilic bacteria in a rotating-drum reactor

    SciTech Connect (OSTI)

    Rodrigues, Michael L.M.; Leão, Versiane A.; Gomes, Otavio; Lambert, Fanny; Bastin, David; Gaydardzhiev, Stoyan

    2015-07-15

    Highlights: • Copper bioleaching from PCB (20 mm) by moderate thermophiles was demonstrated. • Larger PCB sheets enable a cost reduction due to the elimination of fine grinding. • Crushing generated cracks in PCB increasing the copper extraction. • A pre-treatment step was necessary to remove the lacquer coating. • High copper extractions (85%) were possible with pulp density of up to 25.0 g/L. - Abstract: The current work reports on a new approach for copper bioleaching from Printed Circuit Board (PCB) by moderate thermophiles in a rotating-drum reactor. Initially leaching of PCB was carried out in shake flasks to assess the effects of particle size (−208 μm + 147 μm), ferrous iron concentration (1.25–10.0 g/L) and pH (1.5–2.5) on copper leaching using mesophile and moderate thermophile microorganisms. Only at a relatively low solid content (10.0 g/L) complete copper extraction was achieved from the particle size investigated. Conversely, high copper extractions were possible from coarse-ground PCB (20 mm-long) working with increased solids concentration (up to 25.0 g/L). Because there was as the faster leaching kinetics at 50 °C Sulfobacillus thermosulfidooxidans was selected for experiments in a rotating-drum reactor with the coarser-sized PCB sheets. Under optimal conditions, copper extraction reached 85%, in 8 days and microscopic observations by SEM–EDS of the on non-leached and leached material suggested that metal dissolution from the internal layers was restricted by the fact that metal surface was not entirely available and accessible for the solution in the case of the 20 mm-size sheets.

  8. Biomass growth restriction in a packed bed reactor

    DOE Patents [OSTI]

    Griffith, William L.; Compere, Alicia L.

    1978-01-01

    When carrying out continuous biologically catalyzed reactions with anaerobic microorganisms attached to a support in an upflow packed bed column, growth of the microorganisms is restricted to prevent the microorganisms from plugging the column by limiting the availability of an essential nutrient and/or by the presence of predatory protozoa which consume the anaerobic microorganisms. A membrane disruptive detergent may be provided in the column to lyse dead microorganisms to make them available as nutrients for live microorganisms.

  9. Enhanced-locality fiber-optic two-photon-fluorescence live-brain interrogation

    SciTech Connect (OSTI)

    Fedotov, I. V.; Doronina-Amitonova, L. V.; Sidorov-Biryukov, D. A.; Fedotov, A. B.; Anokhin, K. V.; Kilin, S. Ya.; Sakoda, K.; Zheltikov, A. M.

    2014-02-24

    Two-photon excitation is shown to substantially enhance the locality of fiber-based optical interrogation of strongly scattering biotissues. In our experiments, a high-numerical-aperture, large-core-are fiber probe is used to deliver the 200-fs output of a 100-MHz mode-locked ytterbium fiber laser to samples of live mouse brain, induce two-photon fluorescence of nitrogen–vacancy centers in diamond markers in brain sample. Fiber probes with a high numerical aperture and a large core area are shown to enable locality enhancement in fiber-laser–fiber-probe two-photon brain excitation and interrogation without sacrificing the efficiency of fluorescence response collection.

  10. Removal of long-lived {sup 222}Rn daughters by electropolishing thin layers of stainless steel

    SciTech Connect (OSTI)

    Schnee, R. W.; Bowles, M. A.; Bunker, R.; McCabe, K.; White, J. [Department of Physics, Syracuse University, Syracuse, NY 13244 (United States)] [Department of Physics, Syracuse University, Syracuse, NY 13244 (United States); Cushman, P.; Pepin, M. [School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455 (United States)] [School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455 (United States); Guiseppe, V. E. [University of South Dakota, Vermillion, South Dakota 57069 (United States)] [University of South Dakota, Vermillion, South Dakota 57069 (United States)

    2013-08-08

    Long-lived alpha and beta emitters in the {sup 222}Rn decay chain on detector surfaces may be the limiting background in many experiments attempting to detect dark matter or neutrinoless double beta decay. Removal of tens of microns of material via electropolishing has been shown to be effective at removing radon daughters implanted into material surfaces. Some applications, however, require the removal of uniform and significantly smaller thicknesses. Here, we demonstrate that electropolishing < 1 ?m from stainless-steel plates reduces the contamination efficiently, by a factor > 100. Examination of electropolished wires with a scanning electron microscope confirms that the thickness removed is reproducible and reasonably uniform. Together, these tests demonstrate the effectiveness of removal of radon daughters for a proposed low-radiation, multi-wire proportional chamber (the BetaCage), without compromising the screeners energy resolution. More generally, electropolishing thin layers of stainless steel may effectively remove radon daughters without compromising precision-machined parts.

  11. Long-lived light mediator to dark matter and primordial small scale spectrum

    SciTech Connect (OSTI)

    Zhang, Yue

    2015-05-06

    We calculate the early universe evolution of perturbations in the dark matter energy density in the context of simple dark sector models containing a GeV scale light mediator. We consider the case that the mediator is long-lived, with lifetime up to a second, and before decaying it temporarily dominates the energy density of the universe. We show that for primordial perturbations that enter the horizon around this period, the interplay between linear growth during matter domination and collisional damping can generically lead to a sharp peak in the spectrum of dark matter density perturbation. As a result, the population of the smallest DM halos gets enhanced. Possible implications of this scenario are discussed.

  12. Technology Solutions Case Study: Air Leakage and Air Transfer Between Garage and Living Space, Waldorf, Maryland

    SciTech Connect (OSTI)

    2014-11-01

    In this project, Building Science Corporation worked with production homebuilder K. Hovnanian to evaluate air transfer between the garage and living space in a single-family detached home constructed by a production homebuilder in compliance with the 2009 International Residential Code and the 2009 International Energy Conservation Code. The project gathered important information about the performance of whole-building ventilation systems and garage ventilation systems as they relate to minimizing flow of contaminated air from garage to living space. A series of 25 multipoint fan pressurization tests and additional zone pressure diagnostic testing measured the garage and house air leakage, the garage-to-house air leakage, and garage and house pressure relationships to each other and to outdoors using automated fan pressurization and pressure monitoring techniques. While the relative characteristics of this house may not represent the entire population of new construction configurations and air tightness levels (house and garage) throughout the country, the technical approach was conservative and should reasonably extend the usefulness of the results to a large spectrum of house configurations from this set of parametric tests in this one house. Based on the results of this testing, the two-step garage-to-house air leakage test protocol described above is recommended where whole-house exhaust ventilation is employed. For houses employing whole-house supply ventilation (positive pressure) or balanced ventilation (same pressure effect as the baseline condition), adherence to the EPA Indoor airPLUS house-to-garage air sealing requirements should be sufficient to expect little to no garage-to-house air transfer.

  13. A search for long-lived particles that stop in the CMS detector and decay to muons

    SciTech Connect (OSTI)

    Alimena, Juliette

    2016-01-01

    A search for long-lived particles that are produced in proton-proton collisions at the CERN LHC, come to rest in the CMS detector, and decay to muons is presented. The decays of the stopped particles could be observed during the intervals between LHC beam crossings, at times that are well separated from any proton-proton collisions. The analysis uses 19.7 1/fb of 8 TeV data collected by CMS in 2012, during a search interval of 293 hours of trigger livetime. Massive, long-lived particles do not exist in the Standard Model, and so any sign of them would be an indication of new physics. The results are interpreted with a model that predicts a long-lived particle that has a charge of twice the electron charge and that behaves like a lepton. Cross section limits are set for each long-lived particle mass as a function of lifetime, for lifetimes between 100 ns and 10 days. These are the first limits for long-lived stopped particles that decay to muons.

  14. Processing of cellulosic material by a cellulase-containing cell-free fermentate produced from cellulase-producing bacteria, ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig

    1998-01-01

    Bacteria which produce large amounts of a cellulase-containing cell-free fermentate, have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase degrading bacterium ATCC 55702, which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic materials.

  15. Processing of cellulosic material by a cellulase-containing cell-free fermentate produced from cellulase-producing bacteria, ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1998-08-04

    Bacteria which produce large amounts of a cellulase-containing cell-free fermentate, have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase degrading bacterium ATCC 55702, which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic materials. 5 figs.

  16. Preliminary investigation of the effects of mineralogy and fluid composition on the growth of thermophilic bacteria in geothermal hot springs on the island of Vulcano, Italy

    SciTech Connect (OSTI)

    Amend, J.P.; Helgeson, H.C. . Dept. of Geology and Geophysics); Gurrieri, S.; Valenza, M. ); Clark, D.S. . Dept. of Chemical Engineering)

    1992-01-01

    Hydrothermal experiments were carried out recently on the island of Vulcano to investigate at in situ temperatures the relation of thermophilic bacterial growth to the mineralogy and fluid chemistry of geothermal hot springs. A preheated nutrient medium was inoculated with geothermal fluid and placed in the hydrothermal reactor, together with a sample of the mineralogic matrix through which the fluid flows. The results of the experiments are somewhat equivocal owing to (1) the inability to maintain the pH of the reactor fluid at the in situ pH (2.9 at 98 C), (2) apparent phase separation of what is probably a CO[sub 2]-rich gas leading to abnormally high pressures as the reactor temperature was increased in stages to 125 C, and (3) the fact that (unexpectedly) all of the bacteria were found to occur on the surfaces of mineral grains, which could not be sequentially collected in a representative manner with the apparatus at hand. Nevertheless, it appeared qualitatively that the population of bacteria increased during the experiment. Although this observation requires future confirmation and quantification with a more sophisticated reactor, the experimental results clearly indicate that conventional microbiological growth experiments using thermophilic bacteria that have been removed from their natural nutrient, in situ pH, and mineralogic environment may have little to do with the behavior of such bacteria in geothermal systems. Understanding this behavior requires integrated studies of the organobiogeochemistry of geothermal systems.

  17. Realistic fission models, new beta-decay half-lives and the r-process in neutron star mergers

    SciTech Connect (OSTI)

    Shibagaki, S.; Kajino, T. [Department of Astronomy, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan and National Astronomical Observatory of Japan, 2-21-1 Osawa, Mitaka, Tokyo 181-8588 (Japan); Chiba, S. [Research Laboratory for Nuclear Reactors, Tokyo Institute of Technology, Ookayama, Meguro-ku, Tokyo, 152-8850 (Japan); Lorusso, G.; Nishimura, S. [RIKEN Nishina Center, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan); Mathews, G. J. [Center for Astrophysics, Department of Physics, University of Notre Dame, IN 46556 (United States)

    2014-05-02

    Almost half of heavy nuclei beyond iron are considered to be produced by rapid neutron capture process (r-process). This process occurs in the neutron-rich environment such as core-collapse supernovae or neutron star mergers, but the main production site is still unknown. In the r-process of neutron star mergers, nuclear fission reactions play an important role. Also beta-decay half-lives of magic nuclei are crucial for the r-process. We have carried out r-process nucleosynthesis calculations based upon new theoretical estimates of fission fragment distributions and new beta-decay half-lives for N=82 nuclei measured at RIBF-RIKEN. We investigate the effect of nuclear fission on abundance patterns in the matter ejected from neutron star mergers with two different fission fragment mass distributions. We also discuss how the new experimental beta-decay half-lives affect the r-process.

  18. Bioremediation of nanomaterials (Patent) | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    In some embodiment, the microorganisms are bacterial organisms such as Gram negative bacteria, which are used as model organisms to study the nanotoxicity of the fullerene ...

  19. Phase Preference by Active, Acetate-Utilizing Bacteria at the Rifle, CO Integrated Field Research Challenge Site

    SciTech Connect (OSTI)

    Kerkhof, L.; Williams, K.H.; Long, P.E.; McGuinness, L.

    2011-02-21

    Previous experiments at the Rifle, Colorado Integrated Field Research Challenge (IFRC) site demonstrated that field-scale addition of acetate to groundwater reduced the ambient soluble uranium concentration. In this report, sediment samples collected before and after acetate field addition were used to assess the active microbes via {sup 13}C acetate stable isotope probing on 3 phases [coarse sand, fines (8-approximately 150 {micro}m), groundwater (0.2-8 {micro}m)] over a 24-day time frame. TRFLP results generally indicated a stronger signal in {sup 13}C-DNA in the 'fines' fraction compared to the sand and groundwater. Before the field-scale acetate addition, a Geobacter-like group primarily synthesized {sup 13}C-DNA in the groundwater phase, an alpha Proteobacterium primarily grew on the fines/sands, and an Acinetobacter sp. and Decholoromonas-like OTU utilized much of the {sup 13}C acetate in both groundwater and particle-associated phases. At the termination of the field-scale acetate addition, the Geobacter-like species was active on the solid phases rather than the groundwater, while the other bacterial groups had very reduced newly synthesized DNA signal. These findings will help to delineate the acetate utilization patterns of bacteria in the field and can lead to improved methods for stimulating distinct microbial populations in situ.

  20. Anaerobic thermophilic bacteria isolated from a Venezuelan oil field and its potential use in microbial improved oil recovery

    SciTech Connect (OSTI)

    Trebbau, G.; Fernandez, B.; Marin, A.

    1995-12-31

    The objective of this work is to determine the ability of indigenous bacteria from a Venezuelan oil field to grow under reservoir conditions inside a porous media, and to produce metabolites capable of recovering residual crude oil. For this purpose, samples of formation waters from a central-eastern Venezuelan oil reservoir were enriched with different carbon sources and a mineral basal media. Formation water was used as a source of trace metals. The enrichments obtained were incubated at reservoir temperature (71{degrees}C), reservoir pressure (1,200 psi), and under anaerobic conditions for both outside and inside porous media (Berea core). Growth and metabolic activity was followed outside porous media by measuring absorbance at 660 nm, increases in pressure, and decreases in pH. Inside porous media bacterial activity was determined by visual examination of the produced waters (gas bubbles and bacterial cells). All the carbohydrates tested outside porous media showed good growth at reservoir conditions. The pH was lowered, gases such as CO{sub 2} and CH{sub 4} were identified by GC. Surface tension was lowered in some enrichments by 30% when compared to controls. Growth was decreased inside porous media, but gases were produced and helped displace oil. In addition, 10% residual oil was recovered from the Berea core. Mathematical modeling was applied to the laboratory coreflood experiment to evaluate the reproducibility of the results obtained.

  1. SEALING LARGE-DIAMETER CAST-IRON PIPE JOINTS UNDER LIVE CONDITIONS

    SciTech Connect (OSTI)

    Kiran M. Kothari; Gerard T. Pittard

    2005-07-01

    Utilities in the U.S. operate over 75,000 km (47,000 miles) of old cast-iron pipes for gas distribution. Bell-and-spigot joints that connect pipe sections together tend to leak as these pipes age. Current repair practices are costly and highly disruptive. The objective of this program is to design, test and commercialize a robotic system capable of sealing multiple castiron bell and spigot joints from a single pipe entry point. The proposed system will perform repairs with the pipe in service by traveling through the pipe, cleaning each joint surface, and installing a stainless-steel sleeve lined with an epoxy-impregnated felt across the joint. This approach will save considerable time and labor, minimize excavation, avoid traffic disruption, and eliminate any requirement to interrupt service to customers (which would result in enormous expense to utilities). Technical challenges include: (1) repair sleeves must compensate for diametric variation and eccentricity of old cast-iron pipes; (2) the assembly must travel long distances through pipes containing debris; (3) the pipe wall must be effectively cleaned in the immediate area of the joint to assure good bonding of the sleeve; and (4) an innovative bolt-on entry fitting is required to conduct safe repair operations on live mains.

  2. Measurement of the body composition of living gray seals by hydrogen isotope dilution

    SciTech Connect (OSTI)

    Reilly, J.J.; Fedak, M.A. )

    1990-09-01

    The body composition of living gray seals (Halichoerus grypus) can be accurately predicted from a two-step model that involves measurement of total body water (TBW) by {sup 2}H or {sup 3}H dilution and application of predictive relationships between body components and TBW that were derived empirically by slaughter chemical analysis. TBW was overestimated by both {sup 2}HHO and {sup 3}HHO dilution; mean overestimates were 2.8 +/- 0.9% (SE) with 2H and 4.0 +/- 0.6% with {sup 3}H. The relationships for prediction of total body fat (TBF), protein (TBP), gross energy (TBGE), and ash (TBA) were as follows: %TBF = 105.1 - 1.47 (%TBW); %TBP = 0.42 (%TBW) - 4.75; TBGE (MJ) = 40.8 (mass in kg) - 48.5 (TBW in kg) - 0.4; and TBA (kg) = 0.1 - 0.008 (mass in kg) + 0.05 (TBW in kg). These relationships are applicable to gray seals of both sexes over a wide range of age and body conditions, and they predict the body composition of gray seals more accurately than the predictive equations derived from ringed seals (Pusa hispida) and from the equation of Pace and Rathbun, which has been reported to be generally applicable to mammals.

  3. Search for long-lived particles in e+e- collisions

    SciTech Connect (OSTI)

    Lees, J. P.

    2015-04-29

    In this study, we present a search for a neutral, long-lived particle L that is produced in e+e collisions and decays at a significant distance from the e+e interaction point into various flavor combinations of two oppositely charged tracks. The analysis uses an e+e data sample with a luminosity of 489.1 fb–1 collected by the BABAR detector at the Υ(4S), Υ(3S), and Υ(2S) resonances and just below the Υ(4S). Fitting the two-track mass distribution in search of a signal peak, we do not observe a significant signal, and set 90% confidence level upper limits on the product of the L production cross section, branching fraction, and reconstruction efficiency for six possible two-body L decay modes as a function of the L mass. The efficiency is given for each final state as a function of the mass, lifetime, and transverse momentum of the candidate, allowing application of the upper limits to any production model. In addition, upper limits are provided on the branching fraction B(B → XsL), where Xs is a strange hadronic system.

  4. Dissecting Regional Variations in Stress Fiber Mechanics in Living Cells with Laser Nanosurgery

    SciTech Connect (OSTI)

    Tanner, Kandice; Boudreau, Aaron; Bissell, Mina J; Kumar, Sanjay

    2010-03-02

    The ability of a cell to distribute contractile stresses across the extracellular matrix in a spatially heterogeneous fashion underlies many cellular behaviors, including motility and tissue assembly. Here we investigate the biophysical basis of this phenomenon by using femtosecond laser nanosurgery to measure the viscoelastic recoil and cell-shape contributions of contractile stress fibers (SFs) located in specific compartments of living cells. Upon photodisruption and recoil, myosin light chain kinase-dependent SFs located along the cell periphery display much lower effective elasticities and higher plateau retraction distances than Rho-associated kinase-dependent SFs located in the cell center, with severing of peripheral fibers uniquely triggering a dramatic contraction of the entire cell within minutes of fiber irradiation. Image correlation spectroscopy reveals that when one population of SFs is pharmacologically dissipated, actin density flows toward the other population. Furthermore, dissipation of peripheral fibers reduces the elasticity and increases the plateau retraction distance of central fibers, and severing central fibers under these conditions triggers cellular contraction. Together, these findings show that SFs regulated by different myosin activators exhibit different mechanical properties and cell shape contributions. They also suggest that some fibers can absorb components and assume mechanical roles of other fibers to stabilize cell shape.

  5. High temperature thermoplastic elastomers synthesized by living anionic polymerization in hydrocarbon solvent at room temperature

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Schlegel, Ralf; Williams, Katherine; Voyloy, Dimitry; Steren, Carlos A.; Goodwin, Andrew; Coughlin, E. Bryan; Gido, Samuel; Beiner, Mario; Hong, Kunlun; Kang, Nam -Goo; et al

    2016-03-30

    We present the synthesis and characterization of a new class of high temperature thermoplastic elastomers composed of polybenzofulvene–polyisoprene–polybenzofulvene (FIF) triblock copolymers. All copolymers were prepared by living anionic polymerization in benzene at room temperature. Homopolymerization and effects of additives on the glass transition temperature (Tg) of polybenzofulvene (PBF) were also investigated. Among all triblock copolymers studied, FIF with 14 vol % of PBF exhibited a maximum stress of 14.3 ± 1.3 MPa and strain at break of 1390 ± 66% from tensile tests. The stress–strain curves of FIF-10 and 14 were analyzed by a statistical molecular approach using a nonaffinemore » tube model to estimate the thermoplastic elastomer behavior. Dynamic mechanical analysis showed that the softening temperature of PBF in FIF was 145 °C, much higher than that of thermoplastic elastomers with polystyrene hard blocks. Microphase separation of FIF triblock copolymers was observed by small-angle X-ray scattering, even though long-range order was not achieved under the annealing conditions employed. Additionally, the microphase separation of the resulting triblock copolymers was examined by atomic force microscopy.« less

  6. Efficient Analysis of Live and Historical Streaming Data and itsApplication to Cybersecurity

    SciTech Connect (OSTI)

    Reiss, Frederick; Stockinger, Kurt; Wu, Kesheng; Shoshani, Arie; Hellerstein, Joseph M.

    2007-04-06

    Applications that query data streams in order to identifytrends, patterns, or anomalies can often benefit from comparing the livestream data with archived historical stream data. However, searching thishistorical data in real time has been considered so far to beprohibitively expensive. One of the main bottlenecks is the update costsof the indices over the archived data. In this paper, we address thisproblem by using our highly-efficient bitmap indexing technology (calledFastBit) and demonstrate that the index update operations aresufficiently efficient for this bottleneck to be removed. We describe ourprototype system based on the TelegraphCQ streaming query processor andthe FastBit bitmap index. We present a detailed performance evaluation ofour system using a complex query workload for analyzing real networktraffic data. The combined system uses TelegraphCQ to analyze streams oftraffic information and FastBit to correlate current behaviors withhistorical trends. We demonstrate that our system can simultaneouslyanalyze (1) live streams with high data rates and (2) a large repositoryof historical stream data.

  7. Seasonal succession leads to habitat-dependent differentiation in ribosomal RNA:DNA ratios among freshwater lake bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Denef, Vincent J.; Fujimoto, Masanori; Berry, Michelle A.; Schmidt, Marian L.

    2016-04-29

    Relative abundance profiles of bacterial populations measured by sequencing DNA or RNA of marker genes can widely differ. These differences, made apparent when calculating ribosomal RNA:DNA ratios, have been interpreted as variable activities of bacterial populations. However, inconsistent correlations between ribosomal RNA:DNA ratios and metabolic activity or growth rates have led to a more conservative interpretation of this metric as the cellular protein synthesis potential (PSP). Little is known, particularly in freshwater systems, about how PSP varies for specific taxa across temporal and spatial environmental gradients and how conserved PSP is across bacterial phylogeny. Here, we generated 16S rRNA genemore » sequencing data using simultaneously extracted DNA and RNA from fractionated (free-living and particulate) water samples taken seasonally along a eutrophic freshwater estuary to oligotrophic pelagic transect in Lake Michigan. In contrast to previous reports, we observed frequent clustering of DNA and RNA data from the same sample. Analysis of the overlap in taxa detected at the RNA and DNA level indicated that microbial dormancy may be more common in the estuary, the particulate fraction, and during the stratified period. Across spatiotemporal gradients, PSP was often conserved at the phylum and class levels. PSPs for specific taxa were more similar across habitats in spring than in summer and fall. This was most notable for PSPs of the same taxa when located in the free-living or particulate fractions, but also when contrasting surface to deep, and estuary to Lake Michigan communities. Our results show that community composition assessed by RNA and DNA measurements are more similar than previously assumed in freshwater systems. Furthermore, the similarity between RNA and DNA measurements and taxa-specific PSPs that drive community-level similarities are conditional on spatiotemporal factors.« less

  8. The fate of long-lived superparticles with hadronic decays after LHC Run 1

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Liu, Zhen; Tweedie, Brock

    2015-06-08

    Supersymmetry searches at the LHC are both highly varied and highly constraining, but the vast majority are focused on cases where the final-stage visible decays are prompt. Scenarios featuring superparticles with detector-scale lifetimes have therefore remained a tantalizing possibility for sub-TeV SUSY, since explicit limits are relatively sparse. Nonetheless, the extremely low backgrounds of the few existing searches for collider-stable and displaced new particles facilitates recastings into powerful long-lived superparticle searches, even for models for which those searches are highly non-optimized. In this paper, we assess the status of such models in the context of baryonic R-parity violation, gauge mediation,more » and mini-split SUSY. We then explore a number of common simplified spectra where hadronic decays can be important, employing recasts of LHC searches that utilize different detector systems and final-state objects. The LSP/NLSP possibilities considered here include generic colored superparticles such as the gluino and light-flavor squarks, as well as the lighter stop and the quasi-degenerate Higgsino multiplet motivated by naturalness. We find that complementary coverage over large swaths of mass and lifetime is achievable by superimposing limits, particularly from CMS’s tracker-based displaced dijet search and heavy stable charged particle searches. By adding in prompt searches, we find many cases where a range of sparticle masses is now excluded from zero lifetime to infinite lifetime with no gaps. In other cases, the displaced searches furnish the only extant limits at any lifetime.« less

  9. The fate of long-lived superparticles with hadronic decays after LHC Run 1

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Liu, Zhen; Tweedie, Brock

    2015-06-08

    Supersymmetry searches at the LHC are both highly varied and highly constraining, but the vast majority are focused on cases where the final-stage visible decays are prompt. Scenarios featuring superparticles with detector-scale lifetimes have therefore remained a tantalizing possibility for sub-TeV SUSY, since explicit limits are relatively sparse. Nonetheless, the extremely low backgrounds of the few existing searches for collider-stable and displaced new particles facilitates recastings into powerful long-lived superparticle searches, even for models for which those searches are highly non-optimized. In this paper, we assess the status of such models in the context of baryonic R-parity violation, gauge mediation,moreand mini-split SUSY. We explore a number of common simplified spectra where hadronic decays can be important, employing recasts of LHC searches that utilize different detector systems and final-state objects. The LSP/NLSP possibilities considered here include generic colored superparticles such as the gluino and light-flavor squarks, as well as the lighter stop and the quasi-degenerate Higgsino multiplet motivated by naturalness. We find that complementary coverage over large swaths of mass and lifetime is achievable by superimposing limits, particularly from CMSs tracker-based displaced dijet search and heavy stable charged particle searches. Adding in prompt searches, we find many cases where a range of sparticle masses is now excluded from zero lifetime to infinite lifetime with no gaps. In other cases, the displaced searches furnish the only extant limits at any lifetime.less

  10. MIXING AND TRANSPORT OF SHORT-LIVED AND STABLE ISOTOPES AND REFRACTORY GRAINS IN PROTOPLANETARY DISKS

    SciTech Connect (OSTI)

    Boss, Alan P.

    2013-08-10

    Analyses of primitive meteorites and cometary samples have shown that the solar nebula must have experienced a phase of large-scale outward transport of small refractory grains as well as homogenization of initially spatially heterogeneous short-lived isotopes. The stable oxygen isotopes, however, were able to remain spatially heterogeneous at the {approx}6% level. One promising mechanism for achieving these disparate goals is the mixing and transport associated with a marginally gravitationally unstable (MGU) disk, a likely cause of FU Orionis events in young low-mass stars. Several new sets of MGU models are presented that explore mixing and transport in disks with varied masses (0.016 to 0.13 M{sub Sun }) around stars with varied masses (0.1 to 1 M{sub Sun }) and varied initial Q stability minima (1.8 to 3.1). The results show that MGU disks are able to rapidly (within {approx}10{sup 4} yr) achieve large-scale transport and homogenization of initially spatially heterogeneous distributions of disk grains or gas. In addition, the models show that while single-shot injection heterogeneity is reduced to a relatively low level ({approx}1%), as required for early solar system chronometry, continuous injection of the sort associated with the generation of stable oxygen isotope fractionations by UV photolysis leads to a sustained, relatively high level ({approx}10%) of heterogeneity, in agreement with the oxygen isotope data. These models support the suggestion that the protosun may have experienced at least one FU Orionis-like outburst, which produced several of the signatures left behind in primitive chondrites and comets.

  11. Search for Charged Massive Long-Lived Particles Using the D0 Detector

    SciTech Connect (OSTI)

    Xie, Yunhe; /Brown U.

    2009-05-01

    A search for charged massive stable particles has been performed with the D0 detector using 1.1 fb{sup -1} of data. The speed of the particle has been calculated based on the time-of-flight and position information in the muon system. The present research is limited to direct pair-production of the charged massive long-lived particles. We do not consider CMSPs that result from the cascade decays of heavier particles. In this analysis, the exact values of the model parameters of the entire supersymmetric particle mass spectrum, relevant for cascade decays, are not important. We found no evidence of the signal. 95% CL cross-section upper limits have been set on the pair-productions of the stable scaler tau lepton, the gaugino-like charginos, and the higgsino-like charginos. The upper cross section limits vary from 0.31 pb to 0.04 pb, for stau masses in the range between 60 GeV and 300 GeV. We use the nominal value of the theoretical cross section to set limits on the mass of the pair produced charginos. We exclude the pair-produced stable gaugino-like charginos with mass below 206 GeV, and higgsino-like charginos below 171 GeV, respectively. Although the present sensitivity is insufficient to test the model of the pair produced stable staus, we do set cross section limits which can be applied to the pair production of any charged massive stable particle candidates with similar kinematics. These are the most restrictive limits to the present on the cross sections for CMSPs and the first published from the Tevatron Collider Run II. The manuscript has been published by Physical Review Letters in April 2009 and is available at arXiv as.

  12. The fate of long-lived superparticles with hadronic decays after LHC Run 1

    SciTech Connect (OSTI)

    Liu, Zhen; Tweedie, Brock

    2015-06-08

    Supersymmetry searches at the LHC are both highly varied and highly constraining, but the vast majority are focused on cases where the final-stage visible decays are prompt. Scenarios featuring superparticles with detector-scale lifetimes have therefore remained a tantalizing possibility for sub-TeV SUSY, since explicit limits are relatively sparse. Nonetheless, the extremely low backgrounds of the few existing searches for collider-stable and displaced new particles facilitates recastings into powerful long-lived superparticle searches, even for models for which those searches are highly non-optimized. In this paper, we assess the status of such models in the context of baryonic R-parity violation, gauge mediation, and mini-split SUSY. We explore a number of common simplified spectra where hadronic decays can be important, employing recasts of LHC searches that utilize different detector systems and final-state objects. The LSP/NLSP possibilities considered here include generic colored superparticles such as the gluino and light-flavor squarks, as well as the lighter stop and the quasi-degenerate Higgsino multiplet motivated by naturalness. We find that complementary coverage over large swaths of mass and lifetime is achievable by superimposing limits, particularly from CMSs tracker-based displaced dijet search and heavy stable charged particle searches. Adding in prompt searches, we find many cases where a range of sparticle masses is now excluded from zero lifetime to infinite lifetime with no gaps. In other cases, the displaced searches furnish the only extant limits at any lifetime.

  13. In situ capping for size control of monochalcogenides (ZnS, CdS, and SnS) nanocrystals produced by anaerobic metal-reducing bacteria

    SciTech Connect (OSTI)

    Jang, Gyoung Gug; Jacobs, Christopher B; Ivanov, Ilia N; Joshi, Pooran C; Meyer III, Harry M; Kidder, Michelle; Armstrong, Beth L; Datskos, Panos G; Graham, David E; Moon, Ji Won

    2015-01-01

    Metal monochalcogenide quantum dot nanocrystals of ZnS, CdS and SnS were prepared by anaerobic, metal-reducing bacteria using in situ capping by oleic acid or oleylamine. The capping agent preferentially adsorbs on the surface of the nanocrystal, suppressing the growth process in the early stages, thus leading to production of nanocrystals with a diameter of less than 5 nm.

  14. In situ capping for size control of monochalcogenides (ZnS, CdS, and SnS) nanocrystals produced by anaerobic metal-reducing bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Jang, Gyoung Gug; Jacobs, Christopher B.; Ivanov, Ilia N.; Joshi, Pooran C.; Meyer, III, Harry M.; Kidder, Michelle; Armstrong, Beth L.; Datskos, Panos G.; Graham, David E.; Moon, Ji -Won

    2015-07-24

    Metal monochalcogenide quantum dot nanocrystals of ZnS, CdS and SnS were prepared by anaerobic, metal-reducing bacteria using in situ capping by oleic acid or oleylamine. Furthermore, the capping agent preferentially adsorbs on the surface of the nanocrystal, suppressing the growth process in the early stages, thus leading to production of nanocrystals with a diameter of less than 5 nm.

  15. Wireless Self-powered Visual and NDE Robotic Inspection System for Live Gas Distribution Mains

    SciTech Connect (OSTI)

    Susan Burkett; Hagen Schempf

    2006-01-31

    Carnegie Mellon University (CMU) under contract from Department of Energy/National Energy Technology Laboratory (DoE/NETL) and co-funding from the Northeast Gas Association (NGA), has completed the overall system design of the next-generation Explorer-II (X-II) live gas main NDE and visual inspection robot platform. The design is based on the Explorer-I prototype which was built and field-tested under a prior (also DoE- and NGA co-funded) program, and served as the validation that self-powered robots under wireless control could access and navigate live natural gas distribution mains. The X-II system design ({approx}8 ft. and 66 lbs.) was heavily based on the X-I design, yet was substantially expanded to allow the addition of NDE sensor systems (while retaining its visual inspection capability), making it a modular system, and expanding its ability to operate at pressures up to 750 psig (high-pressure and unpiggable steel-pipe distribution mains). A new electronics architecture and on-board software kernel were added to again improve system performance. A locating sonde system was integrated to allow for absolute position-referencing during inspection (coupled with external differential GPS) and emergency-locating. The power system was upgraded to utilize lithium-based battery-cells for an increase in mission-time. The system architecture now relies on a dual set of end camera-modules to house the 32-bit processors (Single-Board Computer or SBC) as well as the imaging and wireless (off-board) and CAN-based (on-board) communication hardware and software systems (as well as the sonde-coil and -electronics). The drive-module (2 ea.) are still responsible for bracing (and centering) to drive in push/pull fashion the robot train into and through the pipes and obstacles. The steering modules and their arrangement, still allow the robot to configure itself to perform any-angle (up to 90 deg) turns in any orientation (incl. vertical), and enable the live launching and

  16. 10-oxo-12(Z)-octadecenoic acid, a linoleic acid metabolite produced by gut lactic acid bacteria, potently activates PPARγ and stimulates adipogenesis

    SciTech Connect (OSTI)

    Goto, Tsuyoshi; Kim, Young-Il; Furuzono, Tomoya; Takahashi, Nobuyuki; Yamakuni, Kanae; Yang, Ha-Eun; Li, Yongjia; Ohue, Ryuji; Nomura, Wataru; Sugawara, Tatsuya; Yu, Rina; Kitamura, Nahoko; and others

    2015-04-17

    Our previous study has shown that gut lactic acid bacteria generate various kinds of fatty acids from polyunsaturated fatty acids such as linoleic acid (LA). In this study, we investigated the effects of LA and LA-derived fatty acids on the activation of peroxisome proliferator-activated receptors (PPARs) which regulate whole-body energy metabolism. None of the fatty acids activated PPARδ, whereas almost all activated PPARα in luciferase assays. Two fatty acids potently activated PPARγ, a master regulator of adipocyte differentiation, with 10-oxo-12(Z)-octadecenoic acid (KetoA) having the most potency. In 3T3-L1 cells, KetoA induced adipocyte differentiation via the activation of PPARγ, and increased adiponectin production and insulin-stimulated glucose uptake. These findings suggest that fatty acids, including KetoA, generated in gut by lactic acid bacteria may be involved in the regulation of host energy metabolism. - Highlights: • Most LA-derived fatty acids from gut lactic acid bacteria potently activated PPARα. • Among tested fatty acids, KetoA and KetoC significantly activated PPARγ. • KetoA induced adipocyte differentiation via the activation of PPARγ. • KetoA enhanced adiponectin production and glucose uptake during adipogenesis.

  17. First Use of High Charge States for Mass Measurements of Short-Lived Nuclides in a Penning Trap

    SciTech Connect (OSTI)

    Ettenauer, S.; Gallant, A. T.; Dilling, J.; Simon, M. C.; Chaudhuri, A.; Mane, E.; Delheij, P.; Pearson, M. R.; Brunner, T.; Chowdhury, U.; Simon, V. V.; Brodeur, M.; Andreoiu, C.; Audi, G.; Lopez-Urrutia, J. R. Crespo; Ullrich, J.; Gwinner, G.; Lapierre, A.; Lunney, D.; Ringle, R.

    2011-12-30

    Penning trap mass measurements of short-lived nuclides have been performed for the first time with highly charged ions, using the TITAN facility at TRIUMF. Compared to singly charged ions, this provides an improvement in experimental precision that scales with the charge state q. Neutron-deficient Rb isotopes have been charge bred in an electron beam ion trap to q=8-12+ prior to injection into the Penning trap. In combination with the Ramsey excitation scheme, this unique setup creating low energy, highly charged ions at a radioactive beam facility opens the door to unrivaled precision with gains of 1-2 orders of magnitude. The method is particularly suited for short-lived nuclides such as the superallowed {beta} emitter {sup 74}Rb (T{sub 1/2}=65 ms). The determination of its atomic mass and an improved Q{sub EC} value are presented.

  18. Distributions of short-lived radioactive nuclei produced by young embedded star clusters

    SciTech Connect (OSTI)

    Adams, Fred C.; Fatuzzo, Marco; Holden, Lisa

    2014-07-01

    Most star formation in the Galaxy takes place in clusters, where the most massive members can affect the properties of other constituent solar systems. This paper considers how clusters influence star formation and forming planetary systems through nuclear enrichment from supernova explosions, where massive stars deliver short-lived radioactive nuclei (SLRs) to their local environment. The decay of these nuclei leads to both heating and ionization, and thereby affects disk evolution, disk chemistry, and the accompanying process of planet formation. Nuclear enrichment can take place on two spatial scales: (1) within the cluster itself (ℓ ∼ 1 pc), the SLRs are delivered to the circumstellar disks associated with other cluster members. (2) On the next larger scale (ℓ ∼ 2-10 pc), SLRs are injected into the background molecular cloud; these nuclei provide heating and ionization to nearby star-forming regions and to the next generation of disks. For the first scenario, we construct the expected distributions of radioactive enrichment levels provided by embedded clusters. Clusters can account for the SLR mass fractions inferred for the early Solar Nebula, but typical SLR abundances are lower by a factor of ∼10. For the second scenario, we find that distributed enrichment of SLRs in molecular clouds leads to comparable abundances. For both the direct and distributed enrichment processes, the masses of {sup 26}Al and {sup 60}Fe delivered to individual circumstellar disks typically fall in the range 10-100 pM {sub ☉} (where 1 pM {sub ☉} = 10{sup –12} M {sub ☉}). The corresponding ionization rate due to SLRs typically falls in the range ζ{sub SLR} ∼ 1-5 × 10{sup –19} s{sup –1}. This ionization rate is smaller than that due to cosmic rays, ζ{sub CR} ∼ 10{sup –17} s{sup –1}, but will be important in regions where cosmic rays are attenuated (e.g., disk mid-planes).

  19. Long-lived charge carrier generation in ordered films of a covalent perylenediimide–diketopyrrolopyrrole–perylenediimide molecule

    SciTech Connect (OSTI)

    Hartnett, Patrick E.; Dyar, Scott M.; Margulies, Eric A.; Shoer, Leah E.; Cook, Andrew W.; Eaton, Samuel W.; Marks, Tobin J.; Wasielewski, Michael R.

    2015-07-31

    The photophysics of a covalently linked perylenediimide–diketopyrrolopyrrole–perylenediimide acceptor–donor–acceptor molecule (PDI–DPP–PDI, 1) were investigated and found to be markedly different in solution versus in unannealed and solvent annealed films. Photoexcitation of 1 in toluene results in quantitative charge separation in τ = 3.1 ± 0.2 ps, with charge recombination in τ = 340 ± 10 ps, while in unannealed/disordered films of 1, charge separation occurs in τ < 250 fs, while charge recombination displays a multiexponential decay in ~6 ns. The absence of long-lived, charge separation in the disordered film suggests that few free charge carriers are generated. In contrast, upon CH₂Cl₂ vapor annealing films of 1, grazing-incidence X-ray scattering shows that the molecules form a more ordered structure. Photoexcitation of the ordered films results in initial formation of a spin-correlated radical ion pair (electron–hole pair) as indicated by magnetic field effects on the formation of free charge carriers which live for ~4 μs. This result has significant implications for the design of organic solar cells based on covalent donor–acceptor systems and shows that long-lived, charge-separated states can be achieved by controlling intramolecular charge separation dynamics in well-ordered systems.

  20. Long-lived charge carrier generation in ordered films of a covalent perylenediimide–diketopyrrolopyrrole–perylenediimide molecule

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hartnett, Patrick E.; Dyar, Scott M.; Margulies, Eric A.; Shoer, Leah E.; Cook, Andrew W.; Eaton, Samuel W.; Marks, Tobin J.; Wasielewski, Michael R.

    2015-07-31

    The photophysics of a covalently linked perylenediimide–diketopyrrolopyrrole–perylenediimide acceptor–donor–acceptor molecule (PDI–DPP–PDI, 1) were investigated and found to be markedly different in solution versus in unannealed and solvent annealed films. Photoexcitation of 1 in toluene results in quantitative charge separation in τ = 3.1 ± 0.2 ps, with charge recombination in τ = 340 ± 10 ps, while in unannealed/disordered films of 1, charge separation occurs in τ < 250 fs, while charge recombination displays a multiexponential decay in ~6 ns. The absence of long-lived, charge separation in the disordered film suggests that few free charge carriers are generated. In contrast, uponmore » CH₂Cl₂ vapor annealing films of 1, grazing-incidence X-ray scattering shows that the molecules form a more ordered structure. Photoexcitation of the ordered films results in initial formation of a spin-correlated radical ion pair (electron–hole pair) as indicated by magnetic field effects on the formation of free charge carriers which live for ~4 μs. This result has significant implications for the design of organic solar cells based on covalent donor–acceptor systems and shows that long-lived, charge-separated states can be achieved by controlling intramolecular charge separation dynamics in well-ordered systems.« less

  1. Geological Repository Layout for Radioactive High Level Long Lived Waste in Argilite

    SciTech Connect (OSTI)

    Gaussen, J.L.

    2006-07-01

    In the framework of the 1991 French radioactive waste act, ANDRA has studied the feasibility of a geological repository in the argillite layer of the Bure site for high-level long-lived waste. This presentation is focused on the underground facilities that constitute the specific component of this project. The preliminary underground layout, which has been elaborated, is based on four categories of data: - the waste characteristics and inventory; - the geological properties of the host argillite; - the long term performance objectives of the repository; - the specifications in term of operation and reversibility. The underground facilities consist of two types of works: the access works (shafts and drifts) and the disposal cells. The function of the access works is to permit the implementation of two concurrent activities: the nuclear operations (transfer and emplacement of the disposal packages into the disposal cells) and the construction of the next disposal cells. The design of the drifts network which matches up to this function is also influenced by two other specifications: the minimisation of the drift dimensions in order to limit their influence on the integrity of the geological formation and the necessity of a safe ventilation in case of fire. The resulting layout is a network of 4 parallel drifts (2 of them being dedicated to the operation, the other two being dedicated to the construction activities). The average diameter of these access drifts is 7 meters. 4 shafts ensure the link between the surface and the underground. The most important function of the disposal cells is to contribute to the long-term performance of the repository. In this regard, the thermal and geotechnical considerations play an important role. The B wastes (intermediate level wastes) are not (or not very) exothermic. Consequently, the design of their disposal cells result mainly from geotechnical considerations. The disposal packages (made of concrete) are piled up in big

  2. SEALING LARGE-DIAMETER CAST-IRON PIPE JOINTS UNDER LIVE CONDITIONS

    SciTech Connect (OSTI)

    Kiran M. Kothari; Gerard T. Pittard

    2003-01-01

    Utilities in the U.S. operate over 75,000 km (47,000 miles) of old cast-iron pipes for gas distribution. The bell-and-spigot joints tend to leak as these pipes age. Current repair practices are costly and highly disruptive. The objective of this program is to design, test and commercialize a robotic system capable of sealing multiple cast-iron bell and spigot joints from a single pipe entry point. The proposed system will perform repairs while the pipe remains in service by traveling through the pipe, cleaning each joint surface, and attaching a stainless-steel sleeve lined with an epoxy-impregnated felt across the joint. This approach will save considerable time and labor, avoid traffic disruption, and eliminate any requirement to interrupt service (which results in enormous expense to utilities). Technical challenges include: (1) repair sleeves must compensate for diametric variation and eccentricity of cast-iron pipes; (2) the assembly must travel long distances through pipes containing debris; (3) the pipe wall must be effectively cleaned in the immediate area of the joint to assure good bonding of the sleeve; and (4) an innovative bolt-on entry fitting is required to conduct repair operations on live mains. The development effort is divided into eleven tasks. Task 1--Program Management was previously completed. Two reports, one describing the program management plan and the other consisting of the technology assessment, were submitted to the DOE COR in the first quarter. Task 2--Establishment of Detailed Design Specifications and Task 3--Design and Fabricate Ratcheting Stainless-Steel Repair Sleeves are now well underway. First-quarter activities included conducting detailed analyses to determine the capabilities of coiled-tubing locomotion for entering and repairing gas mains and the first design iteration of the joint-sealing sleeve. The maximum horizontal reach of coiled tubing inside a pipeline before buckling prevents further access was calculated for a

  3. SEALING LARGE-DIAMETER CAST-IRON PIPE JOINTS UNDER LIVE CONDITIONS

    SciTech Connect (OSTI)

    Kiran M. Kothari; Gerard T. Pittard

    2003-06-01

    Utilities in the U.S. operate over 75,000 km (47,000 miles) of old cast-iron pipes for gas distribution. The bell-and-spigot joints tend to leak as these pipes age. Current repair practices are costly and highly disruptive. The objective of this program is to design, test and commercialize a robotic system capable of sealing multiple cast-iron bell and spigot joints from a single pipe entry point. The proposed system will perform repairs while the pipe remains in service by traveling through the pipe, cleaning each joint surface, and attaching a stainless-steel sleeve lined with an epoxy-impregnated felt across the joint. This approach will save considerable time and labor, avoid traffic disruption, and eliminate any requirement to interrupt service (which results in enormous expense to utilities). Technical challenges include: (1) repair sleeves must compensate for diametric variation and eccentricity of cast-iron pipes; (2) the assembly must travel long distances through pipes containing debris; (3) the pipe wall must be effectively cleaned in the immediate area of the joint to assure good bonding of the sleeve; and (4) an innovative bolt-on entry fitting is required to conduct repair operations on live mains. The development effort is divided into eleven tasks. Task 1-Program Management was previously completed. Two reports, one describing the program management plan and the other consisting of the technology assessment, were submitted to the DOE COR in the first quarter. Task 2-Establishment of Detailed Design Specifications and Task 3-Design and Fabricate Ratcheting Stainless-Steel Repair Sleeves are now well underway. First-quarter activities included conducting detailed analyses to determine the capabilities of coiled-tubing locomotion for entering and repairing gas mains and the first design iteration of the joint-sealing sleeve. The maximum horizontal reach of coiled tubing inside a pipeline before buckling prevents further access was calculated for a wide

  4. Mathematical modeling of positron emission tomography (PET) data to assess radiofluoride transport in living plants following petiolar administration

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Converse, Alexander K.; Ahlers, Elizabeth O.; Bryan, Tom W.; Hetue, Jackson D.; Lake, Katherine A.; Ellison, Paul A.; Engle, Jonathan W.; Barnhart, Todd E.; Nickles, Robert J.; Williams, Paul H.; et al

    2015-03-15

    Background: Ion transport is a fundamental physiological process that can be studied non-invasively in living plants with radiotracer imaging methods. Fluoride is a known phytotoxic pollutant and understanding its transport in plants after leaf absorption is of interest to those in agricultural areas near industrial sources of airborne fluoride. Here we report the novel use of a commercial, high-resolution, animal positron emission tomography (PET) scanner to trace a bolus of [¹⁸F]fluoride administered via bisected petioles of Brassica oleracea, an established model species, to simulate whole plant uptake of atmospheric fluoride. This methodology allows for the first time mathematical compartmental modelingmore » of fluoride transport in the living plant. Radiotracer kinetics in the stem were described with a single-parameter free- and trapped-compartment model and mean arrival times at different stem positions were calculated from the free-compartment time-activity curves. Results: After initiation of administration at the bisected leaf stalk, [¹⁸F] radioactivity climbed for approximately 10 minutes followed by rapid washout from the stem and equilibration within leaves. Kinetic modeling of transport in the stem yielded a trapping rate of 1.5 +/- 0.3%/min (mean +/- s.d., n = 3), velocity of 2.2 +/- 1.1 cm/min, and trapping fraction of 0.8 +/- 0.5%/cm. Conclusion: Quantitative assessment of physiologically meaningful transport parameters of fluoride in living plants is possible using standard positron emission tomography in combination with petiolar radiotracer administration. Movement of free fluoride was observed to be consistent with bulk flow in xylem, namely a rapid and linear change in position with respect to time. Trapping, likely in the apoplast, was observed. Future applications of the methods described here include studies of transport of other ions and molecules of interest in plant physiology.« less

  5. Evaluation of Beta-Delayed Neutron Emission Probabilities and Half-Lives for Z = 2–28

    SciTech Connect (OSTI)

    Birch, M.; Singh, B.; Dillmann, I.; Abriola, D.; Johnson, T.D.; McCutchan, E.A.; Sonzogni, A.A.

    2015-09-15

    We present an evaluation and compilation of β-delayed neutron probabilities and half-lives for nuclei in the region Z = 2–28 ({sup 8}He–{sup 80}Ni). This article includes the recommended values of these quantities as well as a compiled list of experimental measurements for each nucleus in the region for which β-delayed neutron emission is possible. The literature cut-off for this work is August 15{sup th}, 2015. Some notable cases as well as new standards for β-delayed neutron measurements in this mass region are also discussed.

  6. Comparison of killing of gram-negative and gram-positive bacteria by pure singlet oxygen. [Salmonella typhimurium; Escherichia coli; Sarcina lutea; Staphylococcus aureus; Streptococcus lactis; Streptococcus faecalis

    SciTech Connect (OSTI)

    Dahl, T.A.; Midden, W.R. ); Hartman, P.E. )

    1989-04-01

    Gram-negative and gram-positive bacteria were found to display different sensitivities to pure singlet oxygen generated outside of cells. Killing curves for Salmonella typhimurium and Escherichia coli strains were indicative of multihit killing, whereas curves for Sarcina lutea, Staphylococcus aureus, Streptococcus lactis, and Streptococcus faecalis exhibited single-hit kinetics. The S. typhimurium deep rough strain TA1975, which lacks nearly all of the cell wall lipopolysaccharide coat and manifests concomitant enhancement of penetration by some exogenous substances, responded to singlet oxygen with initially faster inactivation than did the S. typhimurium wild-type strain, although the maximum rates of killing appeared to be quite similar. The structure of the cell wall thus plays an important role in susceptibility to singlet oxygen. The outer membrane-lipopolysaccharide portion of the gram-negative cell wall initially protects the bacteria from extracellular singlet oxygen, although it may also serve as a source for secondary reaction products which accentuate the rates of cell killing. S. typhimurium and E. coli strains lacking the cellular antioxidant, glutathione, showed no difference from strains containing glutathione in response to the toxic effects of singlet oxygen. Strains of Sarcina lutea and Staphylococcus aureus that contained carotenoids, however, were far more resistant to singlet oxygen lethality than were both carotenoidless mutants of the same species and other gram-positive species lacking high levels of protective carotenoids.

  7. The role of pyrimidine and water as underlying molecular constituents for describing radiation damage in living tissue: A comparative study

    SciTech Connect (OSTI)

    Fuss, M. C.; Ellis-Gibbings, L.; Jones, D. B.; Brunger, M. J.; Blanco, F.; Muoz, A.; Limo-Vieira, P.; Garca, G.

    2015-06-07

    Water is often used as the medium for characterizing the effects of radiation on living tissue. However, in this study, charged-particle track simulations are employed to quantify the induced physicochemical and potential biological implications when a primary ionising particle with energy 10?keV strikes a medium made up entirely of water or pyrimidine. Note that pyrimidine was chosen as the DNA/RNA bases cytosine, thymine, and uracil can be considered pyrimidine derivatives. This study aims to assess the influence of the choice of medium on the charged-particle transport, and identify how appropriate it is to use water as the default medium to describe the effects of ionising radiation on living tissue. Based on the respective electron interaction cross sections, we provide a model, which allows the study of radiation effects not only in terms of energy deposition (absorbed dose and stopping power) but also in terms of the number of induced molecular processes. Results of these parameters for water and pyrimidine are presented and compared.

  8. A generalized method for characterization of 235U and 239Pu content using short-lived fission product gamma spectroscopy

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Knowles, Justin R.; Skutnik, Steven E.; Glasgow, David C.; Kapsimalis, Roger J.

    2016-06-23

    Rapid non-destructive assay methods for trace fissile material analysis are needed in both nuclear forensics and safeguards communities. To address these needs, research at the High Flux Isotope Reactor Neutron Activation Analysis laboratory has developed a generalized non-destructive assay method to characterize materials containing fissile isotopes. This method relies on gamma-ray emissions from short-lived fission products and capitalizes off of differences in fission product yields to identify fissile compositions of trace material samples. Although prior work has explored the use of short-lived fission product gamma-ray measurements, the proposed method is the first to provide a holistic characterization of isotopic identification,more » mass ratios, and absolute mass determination. Successful single fissile isotope mass recoveries of less than 6% error have been conducted on standards of 235U and 239Pu as low as 12 nanograms in less than 10 minutes. Additionally, mixtures of fissile isotope standards containing 235U and 239Pu have been characterized as low as 229 nanograms of fissile mass with less than 12% error. The generalizability of this method is illustrated by evaluating different fissile isotopes, mixtures of fissile isotopes, and two different irradiation positions in the reactor. Furthermore, it is anticipated that this method will be expanded to characterize additional fissile nuclides, utilize various irradiation sources, and account for increasingly complex sample matrices.« less

  9. DESIGN, CONSTRUCTION AND FIELD DEMONSTRATION OF EXPLORER: A LONG-RANGE UNTETHERED LIVE GASLINE INSPECTION ROBOT SYSTEM

    SciTech Connect (OSTI)

    Dr. George C. Vradis; Dr. Hagen Schempf

    2002-05-01

    The goal of this program is to construct and demonstrate EXPLORER, a modular, remotely controllable, self-powered, untethered robot system for the inspection of live gas distribution 150 mm (6-inch) to 200 mm (8-inch) diameter mains. The system, which was designed in an earlier effort, is built in a modular fashion in order to accommodate various components intended to accomplish different inspection, repair, sample retrieval, and other in-pipe tasks. The prototype system to be built under this project will include all the basic modules needed by the system, i.e. the locomotion, power storage, wireless communication, and camera. The camera, a solid-state fisheye-type, is used to transmit real-time video to the operator that allows for the live inspection of gas distribution pipes. This module, which incorporates technology developed by NASA, has been designed, constructed and tested in the earlier effort. In the current effort, the full prototype system will be tested in the laboratory followed by two field demonstrations in real applications in NYGAS member utilities' pipes. The purpose for EXPLORER is to be able to access live gas mains, insert the system in the piping network, and remotely ''drive'' it within the gas main and its laterals through distances of five to ten thousand feet. Its adaptable locomotion system allows the robot to function through varying diameter pipes (150 - 200 mm or 6- to 8-inches) and is powered via on-board battery-banks. The presence of fish-eye cameras in both ends of the robot allows the operator to view the forward and circumferential views of the internals live using an above-ground TV. Communication takes place via wireless link between the robot and the launch-chamber used to insert/retrieve the system. This link is based on commercial technology presently employed in wireless telecommunication networks. Communication over long distances as well as battery re-charging will be accomplished without retrieving the robot but

  10. Synchrotron based infrared imaging and spectroscopy via focal plane array on live fibroblasts in D2O enriched medium

    SciTech Connect (OSTI)

    Quaroni, Luca; Zlateva, Theodora; Sarafimov, Blagoj; Kreuzer, Helen W.; Wehbe, Katia; Hegg, Eric L.; Cinque, Gianfelice

    2014-03-26

    We tested the viability of using synchrotron based infrared imaging to study biochemical processes inside living cells. As a model system, we studied fibroblast cells exposed to a medium highly enriched with D2O. We could show that the experimental technique allows us to reproduce at the cellular level measurements that are normally performed on purified biological molecules. We can obtain information about lipid conformation and distribution, kinetics of hydrogen/deuterium exchange, and the formation of concentration gradients of H and O isotopes in water that are associated with cell metabolism. The implementation of the full field technique in a sequential imaging format gives a description of cellular biochemistry and biophysics that contains both spatial and temporal information.

  11. Prevalence of respiratory symptoms and diseases in schoolchildren living in a polluted and in a low polluted area in Israel

    SciTech Connect (OSTI)

    Goren, A.I.; Hellmann, S.

    1988-02-01

    Second and fifth grade schoolchildren living in two communities with different levels of air pollution were studied. The parents of these children filled out ATS-NHLI health questionnaires. The prevalence of reported respiratory symptoms and pulmonary diseases was found to be significantly higher among children growing up in the polluted community (Ashdod) as compared with the low-pollution area (Hadera). Logistic models fitted for the respiratory conditions which differed significantly between both areas of residence also included background variables that could be responsible for these differences. Relative risk values, which were calculated from the logistic models, were in the range of 1.47 for cough without cold to 2.66 for asthma for children from Ashdod, as compared with 1.00 for children from Hadera.

  12. Formation of a long-lived hot field reversed configuration by dynamically merging two colliding high-{beta} compact toroids

    SciTech Connect (OSTI)

    Guo, H. Y.; Binderbauer, M. W.; Barnes, D.; Putvinski, S.; Rostoker, N.; Sevier, L.; Tuszewski, M.; Anderson, M. G.; Andow, R.; Bonelli, L.; Brown, R.; Bui, D. Q.; Bystritskii, V.; Clary, R.; Cheung, A. H.; Conroy, K. D.; Deng, B. H.; Dettrick, S. A.; Douglass, J. D.; Feng, P.

    2011-05-15

    A high temperature field reversed configuration (FRC) has been produced in the newly built, world's largest compact toroid (CT) facility, C-2, by colliding and merging two high-{beta} CTs produced using the advanced field-reversed {theta}-pinch technology. This long-lived, stable merged state exhibits the following key properties: (1) apparent increase in the poloidal flux from the first pass to the final merged state, (2) significantly improved confinement compared to conventional {theta}-pinch FRCs with flux decay rates approaching classical values in some cases, (3) strong conversion from kinetic energy into thermal energy with total temperature (T{sub e} + T{sub i}) exceeding 0.5 keV, predominantly into the ion channel. Detailed modeling using a new 2-D resistive magnetohydrodynamic (MHD) code, LamyRidge, has demonstrated, for the first time, the formation, translation, and merging/reconnection dynamics of such extremely high-{beta} plasmas.

  13. Mapping power-law rheology of living cells using multi-frequency force modulation atomic force microscopy

    SciTech Connect (OSTI)

    Takahashi, Ryosuke; Okajima, Takaharu

    2015-10-26

    We present multi-frequency force modulation atomic force microscopy (AFM) for mapping the complex shear modulus G* of living cells as a function of frequency over the range of 50–500 Hz in the same measurement time as the single-frequency force modulation measurement. The AFM technique enables us to reconstruct image maps of rheological parameters, which exhibit a frequency-dependent power-law behavior with respect to G{sup *}. These quantitative rheological measurements reveal a large spatial variation in G* in this frequency range for single cells. Moreover, we find that the reconstructed images of the power-law rheological parameters are much different from those obtained in force-curve or single-frequency force modulation measurements. This indicates that the former provide information about intracellular mechanical structures of the cells that are usually not resolved with the conventional force measurement methods.

  14. Building America Case Study: Air Leakage and Air Transfer Between Garage and Living Space, Waldorf, Maryland (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2014-11-01

    This research project focused on evaluation of air transfer between the garage and living space in a single-family detached home constructed by a production homebuilder in compliance with the 2009 International Residential Code and the 2009 International Energy Conservation Code. The project gathered important information about the performance of whole-building ventilation systems and garage ventilation systems as they relate to minimizing flow of contaminated air from garage to living space. A series of 25 multi-point fan pressurization tests and additional zone pressure diagnostic testing characterized the garage and house air leakage, the garage-to-house air leakage, and garage and house pressure relationships to each other and to outdoors using automated fan pressurization and pressure monitoring techniques. While the relative characteristics of this house may not represent the entire population of new construction configurations and air tightness levels (house and garage) throughout the country, the technical approach was conservative and should reasonably extend the usefulness of the results to a large spectrum of house configurations from this set of parametric tests in this one house. Based on the results of this testing, the two-step garage-to-house air leakage test protocol described above is recommended where whole-house exhaust ventilation is employed. For houses employing whole-house supply ventilation (positive pressure) or balanced ventilation (same pressure effect as the Baseline condition), adherence to the EPA Indoor airPLUS house-to-garage air sealing requirements should be sufficient to expect little to no garage-to-house air transfer.

  15. Explorer-II: Wireless Self-Powered Visual and NDE Robotic Inspection System for Live Gas Distribution Mains

    SciTech Connect (OSTI)

    Carnegie Mellon University

    2008-09-30

    Carnegie Mellon University (CMU) under contract from Department of Energy/National Energy Technology Laboratory (DoE/NETL) and co-funding from the Northeast Gas Association (NGA), has completed the overall system design, field-trial and Magnetic Flux Leakage (MFL) sensor evaluation program for the next-generation Explorer-II (X-II) live gas main Non-destructive Evaluation (NDE) and visual inspection robot platform. The design is based on the Explorer-I prototype which was built and field-tested under a prior (also DoE- and NGA co-funded) program, and served as the validation that self-powered robots under wireless control could access and navigate live natural gas distribution mains. The X-II system design ({approx}8 ft. and 66 lbs.) was heavily based on the X-I design, yet was substantially expanded to allow the addition of NDE sensor systems (while retaining its visual inspection capability), making it a modular system, and expanding its ability to operate at pressures up to 750 psig (high-pressure and unpiggable steel-pipe distribution mains). A new electronics architecture and on-board software kernel were added to again improve system performance. A locating sonde system was integrated to allow for absolute position-referencing during inspection (coupled with external differential GPS) and emergency-locating. The power system was upgraded to utilize lithium-based battery-cells for an increase in mission-time. The resulting robot-train system with CAD renderings of the individual modules. The system architecture now relies on a dual set of end camera-modules to house the 32-bit processors (Single-Board Computer or SBC) as well as the imaging and wireless (off-board) and CAN-based (on-board) communication hardware and software systems (as well as the sonde-coil and -electronics). The drive-module (2 ea.) are still responsible for bracing (and centering) to drive in push/pull fashion the robot train into and through the pipes and obstacles. The steering modules

  16. Search for heavy long-lived multi-charged particles in pp collisions at √s = 8  TeV using the ATLAS detector

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    None

    2015-08-08

    A search for heavy long-lived multi-charged particles is performed using the ATLAS detector at the LHC. Data collected in 2012 at √s = 8 TeV from pp collisions corresponding to an integrated luminosity of 20.3 fb-1 are examined. Particles producing anomalously high ionisation, consistent with long-lived massive particles with electric charges from |q| = 2e to |q| = 6e are searched for. No signal candidate events are observed, and 95 % confidence level cross-section upper limits are interpreted as lower mass limits for a Drell–Yan production model. The mass limits range between 660 and 785 GeV.

  17. Efficient Living Energy Grant

    Broader source: Energy.gov [DOE]

    Many of the Office of Energy and Recycling programs, including this one, are currently closed and are not accepting applications. When funding is available, it will be announced here and on the...

  18. Women @ Energy: Kelly Lively

    Broader source: Energy.gov [DOE]

    "Dive in. Don’t be afraid to do the work and ask questions. Initially, I was intimidated going into college, having been out for so long; but I encourage others to go back, it is never too late to learn things.When in the workforce, you have to ask questions and engage. You have to take that risk—to follow the engineering work in the field and be okay with making mistakes."

  19. Living With Wildfire

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Security Administration | (NNSA) team awarded for hydrogen production research Thursday, August 28, 2014 - 1:19pm Three Lawrence Livermore researchers have received the Department of Energy's 2014 Hydrogen Production R&D Award for their research in producing hydrogen photoelectrochemically - by splitting water using sunlight. Shared with collaborators from the National Renewable Energy Laboratory (NREL) and the University of Nevada, Las Vegas (UNLV), the award recognizes the team for its

  20. Living in Los Alamos

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Education Center University of New Mexico - Los Alamos The Arts Fuller Lodge Art Center Los Alamos Little Theatre Reel Deal Movie Theater County Services Aquatic...