National Library of Energy BETA

Sample records for bacteria microorganisms living

  1. Microorganism immobilization

    DOE Patents [OSTI]

    Compere, Alicia L.; Griffith, William L.

    1981-01-01

    Live metabolically active microorganisms are immobilized on a solid support by contacting particles of aggregate material with a water dispersible polyelectrolyte such as gelatin, crosslinking the polyelectrolyte by reacting it with a crosslinking agent such as glutaraldehyde to provide a crosslinked coating on the particles of aggregate material, contacting the coated particles with live microorganisms and incubating the microorganisms in contact with the crosslinked coating to provide a coating of metabolically active microorganisms. The immobilized microorganisms have continued growth and reproduction functions.

  2. Method of separating bacteria from free living amoebae

    DOE Patents [OSTI]

    Tyndall, Richard L. (Clinton, TN)

    1994-01-01

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  3. Microorganisms for producing organic acids

    DOE Patents [OSTI]

    Pfleger, Brian Frederick; Begemann, Matthew Brett

    2014-09-30

    Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.

  4. Proteolysis in hyperthermophilic microorganisms

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ward, Donald E.; Shockley, Keith R.; Chang, Lara S.; Levy, Ryan D.; Michel, Joshua K.; Conners, Shannon B.; Kelly, Robert M.

    2002-01-01

    Proteases are found in every cell, where they recognize and break down unneeded or abnormal polypeptides or peptide-based nutrients within or outside the cell. Genome sequence data can be used to compare proteolytic enzyme inventories of different organisms as they relate to physiological needs for protein modification and hydrolysis. In this review, we exploit genome sequence data to compare hyperthermophilic microorganisms from the euryarchaeotal genus Pyrococcus , the crenarchaeote Sulfolobus solfataricus , and the bacterium Thermotoga maritima . An overview of the proteases in these organisms is given based on those proteases that have been characterized and on putativemore » proteases that have been identified from genomic sequences, but have yet to be characterized. The analysis revealed both similarities and differences in the mechanisms utilized for proteolysis by each of these hyperthermophiles and indicated how these mechanisms relate to proteolysis in less thermophilic cells and organisms.« less

  5. Cellulase producing microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1997-12-30

    Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  6. Cellulase producing microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig (Lenoir City, TN)

    1997-01-01

    Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  7. Contribution of microorganisms to corrosion

    SciTech Connect (OSTI)

    Thorp, K.E.G.; Crasto, A.S. [Univ. of Dayton Research Inst., OH (United States); Gu, J.D.; Mitchell, R. [Harvard Univ., Cambridge, MA (United States). Div. of Applied Sciences

    1997-08-01

    Current metal primers utilized by the US Air Force contain chromates to inhibit corrosion of the underlying metal. These chromates are both highly toxic and carcinogenic and pose a severe health risk to personnel involved in their application, stripping and disposal. Environmentally-friendly primers with chromate replacements have historically performed poorly with respect to corrosion inhibition. The purpose of this study was to investigate the interaction of chromates with microorganisms in an environment not traditionally associated with biologically-enhanced corrosion to determine if corrosion inhibition by a chromate pigment is, in part, through its action as a biocide. Inoculation of panels which had been coated with a nonchromated primer prior to salt fog exposure and storage in humid conditions resulted in a significant growth of filiform corrosion around a scribe mark. The presence of chromate in the primer severely limited the formation of this corrosion. Likewise, in the absence of the inoculation procedure, the extent of corrosion was strongly diminished. These results suggest that the chromate may be acting as a biocide to limit corrosion which is enhanced by the presence of biological activity.

  8. Oil Production by a Consortium of Oleaginous Microorganisms grown on primary effluent wastewater

    SciTech Connect (OSTI)

    Hall, Jacqueline; Hetrick, Mary; French, Todd; Hernandez, Rafael; Donaldson, Janet; Mondala, Andro; Holmes, William

    2011-01-01

    Municipal wastewater could be a potential growth medium that has not been considered for cultivating oleaginous microorganisms. This study is designed to determine if a consortium of oleaginous microorganism can successfully compete for carbon and other nutrients with the indigenous microorganisms contained in primary effluent wastewater. RESULTS: The oleaginous consortium inoculated with indigenous microorganisms reached stationary phase within 24 h, reaching a maximum cell concentration of 0.58 g L -1. Water quality post-oleaginous consortium growth reached a maximum chemical oxygen demand (COD) reduction of approximately 81%, supporting the consumption of the glucose within 8 h. The oleaginous consortium increased the amount of oil produced per gram by 13% compared with indigenous microorganisms in raw wastewater. Quantitative polymerase chain reaction (qPCR) results show a substantial population increase in bacteria within the first 24 h when the consortium is inoculated into raw wastewater. This result, along with the fatty acid methyl esters (FAMEs) results, suggests that conditions tested were not sufficient for the oleaginous consortium to compete with the indigenous microorganisms.

  9. Live Status

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    For Users Live Status Queue Look Classic Queue Look Scheduled Outages Outage Log Science Gateway Status Login Node Status Filesystem Status My NERSC Move to CRT Getting Started...

  10. Bacteria isolated from amoebae/bacteria consortium

    DOE Patents [OSTI]

    Tyndall, Richard L. (Clinton, TN)

    1995-01-01

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  11. Bacteria isolated from amoebae/bacteria consortium

    DOE Patents [OSTI]

    Tyndall, R.L.

    1995-05-30

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  12. Engineered microorganisms having resistance to ionic liquids

    DOE Patents [OSTI]

    Ruegg, Thomas Lawrence; Thelen, Michael P.

    2016-03-22

    The present invention provides for a method of genetically modifying microorganisms to enhance resistance to ionic liquids, host cells genetically modified in accordance with the methods, and methods of using the host cells in a reaction comprising biomass that has been pretreated with ionic liquids.

  13. Recombinant microorganisms for increased production of organic acids

    DOE Patents [OSTI]

    Yi, Jian (East Lansing, MI); Kleff, Susanne (East Lansing, MI); Guettler, Michael V. (Holt, MI)

    2012-02-21

    Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

  14. Recombinant microorganisms for increased production of organic acids

    DOE Patents [OSTI]

    Yi, Jian; Kleff, Susanne; Guettler, Michael V

    2013-04-30

    Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

  15. Live pathogens: rapid detection technique developed

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Live Pathogens: Rapid Detection Technique Developed Live pathogens: rapid detection technique developed The technique relies on bacteria being critically dependent upon the key nutrient iron. January 24, 2013 Colorized scanning electron micrograph of E. coli. Colorized scanning electron micrograph of E. coli. Photo credit: US Centers for Disease Control and Prevention LANL's new method eliminates the need for laboratory culture and greatly speeds the process. Los Alamos researchers have

  16. Storing data encoded DNA in living organisms

    DOE Patents [OSTI]

    Wong; Pak C. (Richland, WA), Wong; Kwong K. (Sugar Land, TX), Foote; Harlan P. (Richland, WA)

    2006-06-06

    Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

  17. Electrosynthesis of Organic Compounds from Carbon Dioxide Is Catalyzed by a Diversity of Acetogenic Microorganisms

    SciTech Connect (OSTI)

    Nevin, KP; Hensley, SA; Franks, AE; Summers, ZM; Ou, JH; Woodard, TL; Snoeyenbos-West, OL; Lovley, DR

    2011-04-20

    Microbial electrosynthesis, a process in which microorganisms use electrons derived from electrodes to reduce carbon dioxide to multicarbon, extracellular organic compounds, is a potential strategy for capturing electrical energy in carbon-carbon bonds of readily stored and easily distributed products, such as transportation fuels. To date, only one organism, the acetogen Sporomusa ovata, has been shown to be capable of electrosynthesis. The purpose of this study was to determine if a wider range of microorganisms is capable of this process. Several other acetogenic bacteria, including two other Sporomusa species, Clostridium ljungdahlii, Clostridium aceticum, and Moorella thermoacetica, consumed current with the production of organic acids. In general acetate was the primary product, but 2-oxobutyrate and formate also were formed, with 2-oxobutyrate being the predominant identified product of electrosynthesis by C. aceticum. S. sphaeroides, C. ljungdahlii, and M. thermoacetica had high (> 80%) efficiencies of electrons consumed and recovered in identified products. The acetogen Acetobacterium woodii was unable to consume current. These results expand the known range of microorganisms capable of electrosynthesis, providing multiple options for the further optimization of this process.

  18. Engineered microorganisms capable of producing target compounds under anaerobic conditions

    DOE Patents [OSTI]

    Buelter, Thomas; Meinhold, Peter; Feldman, Reid M. Renny; Hawkins, Andrew C.; Urano, Jun; Bastian, Sabine; Arnold, Frances

    2012-01-17

    The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

  19. Bioprocessing of lignite coals using reductive microorganisms

    SciTech Connect (OSTI)

    Crawford, D.L.

    1992-03-29

    In order to convert lignite coals into liquid fuels, gases or chemical feedstock, the macromolecular structure of the coal must be broken down into low molecular weight fractions prior to further modification. Our research focused on this aspect of coal bioprocessing. We isolated, characterized and studied the lignite coal-depolymerizing organisms Streptomyces viridosporus T7A, Pseudomonas sp. DLC-62, unidentified bacterial strain DLC-BB2 and Gram-positive Bacillus megaterium strain DLC-21. In this research we showed that these bacteria are able to solubilize and depolymerize lignite coals using a combination of biological mechanisms including the excretion of coal solublizing basic chemical metabolites and extracellular coal depolymerizing enzymes.

  20. RNA polymerase gene, microorganism having said gene and the production of RNA polymerase by the use of said microorganism

    DOE Patents [OSTI]

    Kotani, Hirokazu (Muko, JP); Hiraoka, Nobutsugu (Muko, JP); Obayashi, Akira (Uji, JP)

    1991-01-01

    SP6 bacteriophage RNA polymerase is produced by cultivating a new microorganism (particularly new strains of Escherichia coli) harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene and recovering SP6 bacteriophage RNA polymerase from the culture broth. SP6 bacteriophage RNA polymerase gene is provided as are new microorganisms harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene.

  1. Apparatus and method for the desulfurization of petroleum by bacteria

    DOE Patents [OSTI]

    Lizama, Hector M.; Scott, Timothy C.; Scott, Charles D.

    1995-01-01

    A method for treating petroleum with anaerobic microorganisms acting as biocatalysts that can remove sulfur atoms from hydrocarbon molecules, under anaerobic conditions, and then convert the sulfur atoms to hydrogen sulfide. The microorganisms utilized are from the family known as the "Sulfate Reducing Bacteria." These bacteria generate metabolic energy from the oxidation of organic compounds, but use oxidized forms of sulfur as an electron acceptor. Because the biocatalyst is present in the form of bacteria in an aqueous suspension, whereas the reacting substrate consists of hydrocarbon molecules in an organic phase, the actual desulfurization reaction takes place at the aqueous-organic interphase. To ensure adequate interfacial contacting and mass transfer, a biphasic electrostatic bioreactor system is utilized. The bioreactor is utilized to disperse and recoalesce a biocatalyst contained in the aqueous liquid phase into the organic liquid phase containing the sulfur. High-intensity electrical fields rupture the aqueous drops into a plurality of microdroplets and induce continuous coalescence and redispersion as the microdroplets travel through the organic phase, thus increasing surface area. As the aqueous microdroplets progress through the organic phase, the biocatalyst then reacts with the sulfur to produce hydrogen sulfide which is then removed from the bioreactor. The organic liquid, now free of the sulfur, is ready for immediate use or further processing.

  2. Apparatus and method for the desulfurization of petroleum by bacteria

    DOE Patents [OSTI]

    Lizama, H.M.; Scott, T.C.; Scott, C.D.

    1995-10-17

    A method is described for treating petroleum with anaerobic microorganisms acting as biocatalysts that can remove sulfur atoms from hydrocarbon molecules, under anaerobic conditions, and then convert the sulfur atoms to hydrogen sulfide. The microorganisms utilized are from the family known as the ``Sulfate Reducing Bacteria``. These bacteria generate metabolic energy from the oxidation of organic compounds, but use oxidized forms of sulfur as an electron acceptor. Because the biocatalyst is present in the form of bacteria in an aqueous suspension, whereas the reacting substrate consists of hydrocarbon molecules in an organic phase, the actual desulfurization reaction takes place at the aqueous-organic interphase. To ensure adequate interfacial contacting and mass transfer, a biphasic electrostatic bioreactor system is utilized. The bioreactor is utilized to disperse and recoalesce a biocatalyst contained in the aqueous liquid phase into the organic liquid phase containing the sulfur. High-intensity electrical fields rupture the aqueous drops into a plurality of microdroplets and induce continuous coalescence and redispersion as the microdroplets travel through the organic phase, thus increasing surface area. As the aqueous microdroplets progress through the organic phase, the biocatalyst then reacts with the sulfur to produce hydrogen sulfide which is then removed from the bioreactor. The organic liquid, now free of the sulfur, is ready for immediate use or further processing. 5 figs.

  3. Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism

    DOE Patents [OSTI]

    Gaddy, James L. (Fayetteville, AR); Clausen, Edgar C. (Fayetteville, AR)

    1992-01-01

    A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H.sub.2 O and/or CO.sub.2 and H.sub.2 in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate.

  4. Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism

    DOE Patents [OSTI]

    Gaddy, J.L.; Clausen, E.C.

    1992-12-22

    A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H[sub 2]O and/or CO[sub 2] and H[sub 2] in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate. 3 figs.

  5. Living a Sustainable Future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Living a Sustainable Future Living a Sustainable Future August 1, 2013 Biomass to fuel project The Laboratory's biomass team is working to solve the energy crisis through...

  6. Microorganisms having enhanced resistance to acetate and methods of use

    DOE Patents [OSTI]

    Brown, Steven D; Yang, Shihui

    2014-10-21

    The present invention provides isolated or genetically modified strains of microorganisms that display enhanced resistance to acetate as a result of increased expression of a sodium proton antiporter. The present invention also provides methods for producing such microbial strains, as well as related promoter sequences and expression vectors. Further, the present invention provides methods of producing alcohol from biomass materials by using microorganisms with enhanced resistance to acetate.

  7. Microorganisms having enhanced tolerance to inhibitors and stress

    DOE Patents [OSTI]

    Brown, Steven D.; Yang, Shihui

    2014-07-29

    The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.

  8. Proteolysis in hyperthermophilic microorganisms (Journal Article) | SciTech

    Office of Scientific and Technical Information (OSTI)

    Connect Proteolysis in hyperthermophilic microorganisms Citation Details In-Document Search Title: Proteolysis in hyperthermophilic microorganisms Proteases are found in every cell, where they recognize and break down unneeded or abnormal polypeptides or peptide-based nutrients within or outside the cell. Genome sequence data can be used to compare proteolytic enzyme inventories of different organisms as they relate to physiological needs for protein modification and hydrolysis. In this

  9. Material to Efficiently and Economically Obtain Microorganism and

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Microalgae - Energy Innovation Portal Biomass and Biofuels Biomass and Biofuels Advanced Materials Advanced Materials Find More Like This Return to Search Material to Efficiently and Economically Obtain Microorganism and Microalgae Ames Laboratory Contact AMES About This Technology Technology Marketing SummaryTechnology provides an economical and efficient process to harvest microorganisms like microalgae from its growth media.Description The interest in using algae as feedstock for biofuel

  10. Microorganisms to Speed Production of Biofuels - Energy Innovation Portal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Microorganisms to Speed Production of Biofuels Oak Ridge National Laboratory Contact ORNL About This Technology Technology Marketing SummaryResearchers at ORNL developed microorganisms that can quickly overcome the resistance of biomass to breakdown, and improved both the cost and efficiency of the biofuel conversion process.DescriptionConventional biomass pretreatment methods release sugars, weak acids, and metabolic by-products that slow down or even stop fermentation, resulting in slower

  11. Fuel from Bacteria, CO2, Water, and Solar Energy: Engineering a Bacterial Reverse Fuel Cell

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: Harvard is engineering a self-contained, scalable Electrofuels production system that can directly generate liquid fuels from bacteria, carbon dioxide (CO2), water, and sunlight. Harvard is genetically engineering bacteria called Shewanella, so the bacteria can sit directly on electrical conductors and absorb electrical current. This current, which is powered by solar panels, gives the bacteria the energy they need to process CO2 into liquid fuels. The Harvard team pumps this CO2 into the system, in addition to water and other nutrients needed to grow the bacteria. Harvard is also engineering the bacteria to produce fuel molecules that have properties similar to gasoline or diesel fuelmaking them easier to incorporate into the existing fuel infrastructure. These molecules are designed to spontaneously separate from the water-based culture that the bacteria live in and to be used directly as fuel without further chemical processing once theyre pumped out of the tank.

  12. Bioengineering and Coordination of Regulatory Networks and Intracellular Complexes to Maximize Hydrogen Production by Phototrophic Microorganisms

    SciTech Connect (OSTI)

    Tabita, F. Robert [The Ohio State University] [The Ohio State University

    2013-07-30

    In this study, the Principal Investigator, F.R. Tabita has teemed up with J. C. Liao from UCLA. This project's main goal is to manipulate regulatory networks in phototrophic bacteria to affect and maximize the production of large amounts of hydrogen gas under conditions where wild-type organisms are constrained by inherent regulatory mechanisms from allowing this to occur. Unrestrained production of hydrogen has been achieved and this will allow for the potential utilization of waste materials as a feed stock to support hydrogen production. By further understanding the means by which regulatory networks interact, this study will seek to maximize the ability of currently available unrestrained organisms to produce hydrogen. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Moreover, due to their great metabolic versatility, such organisms highly regulate these processes in the cell and since virtually all such capabilities are dispensable, excellent experimental systems to study aspects of molecular control and biochemistry/physiology are available.

  13. Potential of Diazorphic, Endophytic Bacteria Associated with...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Diazorphic, Endophytic Bacteria Associated with Sugarcane for Energycane Production Potential of Diazorphic, Endophytic Bacteria Associated with Sugarcane for Energycane...

  14. Novel microorganism for selective separation of coal from pyrite and ash. Final report

    SciTech Connect (OSTI)

    Misra, M.; Smith, R.W.

    1995-09-01

    The separation of fine coal from ash and pyrite was evaluated using a microorganism Mycobacterium phlei.

  15. Biofuels from Solar Energy and Bacteria: Electrofuels Via Direct Electron Transfer from Electrodes to Microbes

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: UMass is feeding renewable electricity to bacteria to provide the microorganisms with the energy they need to turn carbon dioxide (CO2) directly into liquid fuels. UMass energy-to-fuels conversion process is anticipated to be more efficient than current biofuels approaches in part because this process will leverage the high efficiency of photovoltaics to convert solar energy into electricity. UMass is using bacteria already known to produce biofuel from electric current and CO2 and working to increase the amount of electric current those microorganisms will accept and use for biofuels production. In collaboration with scientists at University of California, San Diego, the UMass team is also investigating the use of hydrogen sulfide as a source of energy to power biofuel production.

  16. Field application of a genetically engineered microorganism for polycyclic aromatic hydrocarbon bioremediation process monitoring and control

    SciTech Connect (OSTI)

    Sayler, G.S.; Cox, C.D.; Ripp, S.; Nivens, D.E.; Werner, C.; Ahn, Y.; Matrubutham, U.; Burlage, R.

    1998-11-01

    On October 30, 1996, the US Environmental Protection Agency (EPA) commenced the first test release of genetically engineered microorganisms (GEMs) for use in bioremediation. The specific objectives of the investigation were multifaceted and include (1) testing the hypothesis that a GEM can be successfully introduced and maintained in a bioremediation process, (2) testing the concept of using, at the field scale, reporter organisms for direct bioremediation process monitoring and control, and (3) acquiring data that can be used in risk assessment decision making and protocol development for future field release applications of GEMs. The genetically engineered strain under investigation is Pseudomonas fluorescens strain HK44 (King et al., 1990). The original P. fluorescens parent strain was isolated from polycyclic aromatic hydrocarbon (PAH) contaminated manufactured gas plant soil. Thus, this bacterium is able to biodegrade naphthalene (as well as other substituted naphthalenes and other PAHs) and is able to function as a living bioluminescent reporter for the presence of naphthalene contamination, its bioavailability, and the functional process of biodegradation. A unique component of this field investigation was the availability of an array of large subsurface soil lysimeters. This article describes the experience associated with the release of a genetically modified microorganism, the lysimeter facility and its associated instrumentation, as well as representative data collected during the first eighteen months of operation.

  17. Living a Sustainable Future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Living a Sustainable Future Living a Sustainable Future August 1, 2013 Biomass to fuel project The Laboratory's biomass team is working to solve the energy crisis through biological methods, including genetically engineering algae and cyanobacteria. Create a Sustainable Future: Living Living a Sustainable Future How our Not-so-ordinary Workers Keep LANL Green How many times can LANL reuse water? Google Earth Tour: Water Reuse

  18. Scientist Take First X-Ray Portraits of Living Cyanobacteria at the LCLS

    SciTech Connect (OSTI)

    2015-02-11

    Researchers from Uppsala University working at the Department of Energy's SLAC National Accelerator Laboratory have captured the first X-ray portraits of living bacteria, detecting signals from features as small as 4 nanometers, or 4 billionths of a meter.

  19. Liquid Fuel from Heat-Loving Microorganisms: H2-Dependent Conversion of CO2 to Liquid Electrofuels by Extremely Thermophilic Archaea

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: NC State is working with the University of Georgia to create Electrofuels from primitive organisms called extremophiles that evolved before photosynthetic organisms and live in extreme, hot water environments with temperatures ranging from 167-212 degrees Fahrenheit The team is genetically engineering these microorganisms so they can use hydrogen to turn carbon dioxide directly into alcohol-based fuels. High temperatures are required to distill the biofuels from the water where the organisms live, but the heat-tolerant organisms will continue to thrive even as the biofuels are being distilledmaking the fuel-production process more efficient. The microorganisms dont require light, so they can be grown anywhereinside a dark reactor or even in an underground facility.

  20. Biofuels from Bacteria, Electricity, and CO2: Biofuels from CO2 Using Ammonia or Iron-Oxidizing Bacteria in Reverse Microbial Fuel Cells

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: Electrofuels Project: Columbia University is using carbon dioxide (CO2) from ambient air, ammoniaan abundant and affordable chemical, and a bacteria called N. europaea to produce liquid fuel. The Columbia University team is feeding the ammonia and CO2 into an engineered tank where the bacteria live. The bacteria capture the energy from ammonia and then use that energy to convert CO2 into a liquid fuel. When the bacteria use up all the ammonia, renewable electricity can regenerate it and pump it back into the systemcreating a continuous fuel-creation cycle. In addition, Columbia University is also working with the bacteria A. ferrooxidans to capture and use energy from ferrous iron to produce liquid fuels from CO2.

  1. Expansion of the Genomic Encyclopedia of Bacteria and Archaea

    SciTech Connect (OSTI)

    Rinke, Christian; Sczyrba, Alex; Malfatti, Stephanie; Lee, Janye; Cheng, Jan-Fang; Stepanauskas, Ramunas; Eisen, Jonathan A.; Hallam, Steven; Inskeep, William P.; Hedlund, Brian P.; Sievert, Stefan M.; Liu, Wen-Tso; Tsiamis, George; Hugenholtz, Philip; Woyke, Tanja

    2011-03-20

    To date the vast majority of bacterial and archaeal genomes sequenced are of rather limited phylogenetic diversity as they were chosen based on their physiology and/ or medical importance. The Genomic Encyclopedia of Bacteria and Archaea (GEBA) project (Wu et al. 2009) is aimed to systematically filling the gaps of the tree of life with phylogenetically diverse reference genomes. However more than 99percent of microorganisms elude current culturing attempts, severely limiting the ability to recover complete or even partial genomes of these largely mysterious species. These limitations gave rise to the GEBA uncultured project. Here we propose to use single cell genomics to massively expand the Genomic Encyclopedia of Bacteria and Archaea by targeting 80 single cell representatives of uncultured candidate phyla which have no or very few cultured representatives. Generating these reference genomes of uncultured microbes will dramatically increase the discovery rate of novel protein families and biological functions, shed light on the numerous underrepresented phyla that likely play important roles in the environment, and will assist in improving the reconstruction of the evolutionary history of Bacteria and Archaea. Moreover, these data will improve our ability to interpret metagenomics sequence data from diverse environments, which will be of tremendous value for microbial ecology and evolutionary studies to come.

  2. Expansion of the Genomic Encyclopedia of Bacteria and Archaea

    SciTech Connect (OSTI)

    Rinke, Christian; Sczyrba, Alex; Malfatti, Stephanie; Lee, Janey; Cheng, Jan-Fang; Stepanauskas, Ramunas; Eisen, Jonathan A.; Hallam, Steven; Inskeep, William P.; Hedlund, Brian P.; Sievert, Stefan M.; Liu, Wen-Tso; Tsiamis, George; Hugenholtz, Philip; Woyke, Tanja

    2011-06-02

    To date the vast majority of bacterial and archaeal genomes sequenced are of rather limited phylogenetic diversity as they were chosen based on their physiology and/ or medical importance. The Genomic Encyclopedia of Bacteria and Archaea (GEBA) project (Wu et al. 2009) is aimed at systematically filling the gaps of the tree of life with phylogenetically diverse reference genomes. However more than 99 percent of microorganisms elude current culturing attempts, severely limiting the ability to recover complete or even partial genomes of these largely mysterious species. These limitations gave rise to the GEBA uncultured project. Here we propose to use single cell genomics to massively expand the Genomic Encyclopedia of Bacteria and Archaea by targeting 80 single cell representatives of uncultured candidate phyla which have no or very few cultured representatives. Generating these reference genomes of uncultured microbes will dramatically increase the discovery rate of novel protein families and biological functions, shed light on the numerous underrepresented phyla that likely play important roles in the environment, and will assist in improving the reconstruction of the evolutionary history of Bacteria and Archaea. Moreover, these data will improve our ability to interpret metagenomics sequence data from diverse environments, which will be of tremendous value for microbial ecology and evolutionary studies to come.

  3. Novel microorganism for selective separation of coal from ash and pyrite; First quarterly technical progress report, September 1, 1993--November 30, 1993

    SciTech Connect (OSTI)

    Misra, M.; Smith, R.W.; Raichur, A.M.

    1993-12-31

    This report summarizes the progress made during the first quarter of the research project entitled ``A Novel Microorganism for Selective Separation of Coal from Ash and Pyrite,`` DOE Grant No. DE-FG22-93PC93215. The objective of this project is to study the effectiveness of a novel hydrophobic microorganism, Mycobacterium phlei (M. phlei), for the selective flocculation of coal from pyrite and ash-forming minerals. During the reporting period, three different coal samples: Illinois No. 6 coal, Kentucky No. 9 coal and Pittsburgh No. 8 coal, were collected to be used in the investigation. The microorganism, M. phlei, was obtained as freeze-dried cultures and the growth characteristics of the bacteria were studied. Scanning electron microphotographs revealed that M. phlei cells are coccal in shape and are approximately 1 {mu}m in diameter. Electrokinetic measurements showed that the Illinois No. 6 and Pittsburgh No. 8 coal samples had an isoelectric point (IEP) around pH 6 whereas M. phlei had an IEP around pH 1.5. Electrokinetic measurements of the ruptured microorganisms exhibited an increase in IEP. The increase in IEP of the ruputured cells was due to the release of fatty acids and polar groups from the cell membrane.

  4. Dynamic Metabolism Studies of Live Bacterial Films

    SciTech Connect (OSTI)

    Majors, Paul D.; Mclean, Jeffrey S.

    2008-11-01

    Bacterial film (biofilm) microbes exist within spatial (nutrient, electron-acceptor, pH, etc.) gradients of their own making. Correspondingly, biofilm bacteria are physiologically and functionally distinct from free-floating bacteria and from their own species at differing biofilm depths. This article describes our efforts to develop noninvasive nuclear magnetic resonance (NMR) technologies for biofilm-metabolism studies. This involves integrating NMR with controlled-cultivation methods to interrogate microbial physiology live and under known growth conditions. NMR is uniquely capable of providing depth-resolved metabolic and transport information in a non-invasive, non-sample-consuming fashion, providing information required for experimental reactive transport studies. We have studied mono-species biofilms relevant to environment remediation and human health. We describe these technologies, discuss their advantages and limitations, and give examples of their application.

  5. Living in Los Alamos

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Living in Los Alamos Living in Los Alamos The enchanting surroundings, extraordinary people, and rich history make Los Alamos so much more than just a place to do great work. Discover the many advantages of living and working in Los Alamos. AROUND LOS ALAMOS Communication KRSN Community Radio Los Alamos Daily Post Los Alamos Monitor Education Los Alamos Public Schools Pajarito Environmental Education Center University of New Mexico - Los Alamos The Arts Fuller Lodge Art Center Los Alamos Little

  6. Living With Wildfire

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Wildfire Our exhibit, Living with Wildfire: A Shared Community Experience, shares personal stories of wildfire so that you can understand the tremendous effect a wildfire has...

  7. NREL Explains the Higher Cellulolytic Activity of a Vital Microorganism -

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    News Releases | NREL Explains the Higher Cellulolytic Activity of a Vital Microorganism Wide range of cellulase modalities in C. thermocellum makes it one of the most efficient biomass degraders February 5, 2016 Researchers at the Energy Department's National Renewable Energy Laboratory (NREL) and the BioEnergy Science Center (BESC) say better understanding of a bacterium could lead to cheaper production of cellulosic ethanol and other advanced biofuels. Their discovery was made during an

  8. Aerobic microorganism for the degradation of chlorinated aliphatic hydrocarbons

    DOE Patents [OSTI]

    Fliermans, Carl B. (Augusta, GA)

    1989-01-01

    A chlorinated aliphatic hydrocarbon-degrading microorganism, having American Type Culture Collection accession numbers ATCC 53570 and 53571, in a biologically pure culture aseptically collected from a deep subsurface habitat and enhanced, mineralizes trichloroethylene and tetrachloroethylene to HCl, H.sub.2 O and Co.sub.2 under aerobic conditions stimulated by methane, acetate, methanol, tryptone-yeast extract, propane and propane-methane.

  9. Cofermentation with Cooperative Microorganisms for More Efficient Biomass

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Conversion - Energy Innovation Portal Startup America Startup America Biomass and Biofuels Biomass and Biofuels Find More Like This Return to Search Cofermentation with Cooperative Microorganisms for More Efficient Biomass Conversion Oak Ridge National Laboratory Contact ORNL About This Technology Technology Marketing SummaryIt is well known that biomass has primarily two sources of fermentable carbohydrates, cellulose and hemicelluloses. Research has been underway for decades aimed at both

  10. Use of Stable Isotopes in Forensic Analysis of Microorganisms

    SciTech Connect (OSTI)

    Kreuzer-Martin, Helen W.; Hegg, Eric L.

    2012-01-18

    The use of isotopic signatures for forensic analysis of biological materials is well-established, and the same general principles that apply to interpretation of stable isotope content of C, N, O, and H apply to the analysis of microorganisms. Heterotrophic microorganisms derive their isotopic content from their growth substrates, which are largely plant and animal products, and the water in their culture medium. Thus the isotope signatures of microbes are tied to their growth environment. The C, N, O, and H isotope ratios of spores have been demonstrated to constitute highly discriminating signatures for sample matching. They can rule out specific samples of media and/or water as possible production media, and can predict isotope ratio ranges of the culture media and water used to produce a given sample. These applications have been developed and tested through analyses of approximately 250 samples of Bacillus subtilis spores and over 500 samples of culture media, providing a strong statistical basis for data interpretation. A Bayesian statistical framework for integrating stable isotope data with other types of signatures derived from microorganisms has been able to characterize the culture medium used to produce spores of various Bacillus species, leveraging isotopic differences in different medium types and demonstrating the power of data integration for forensic investigations.

  11. Computational modeling of drug-resistant bacteria. Final report

    SciTech Connect (OSTI)

    MacDougall, Preston

    2015-03-12

    Initial proposal summary: The evolution of antibiotic-resistant mutants among bacteria (superbugs) is a persistent and growing threat to public health. In many ways, we are engaged in a war with these microorganisms, where the corresponding arms race involves chemical weapons and biological targets. Just as advances in microelectronics, imaging technology and feature recognition software have turned conventional munitions into smart bombs, the long-term objectives of this proposal are to develop highly effective antibiotics using next-generation biomolecular modeling capabilities in tandem with novel subatomic feature detection software. Using model compounds and targets, our design methodology will be validated with correspondingly ultra-high resolution structure-determination methods at premier DOE facilities (single-crystal X-ray diffraction at Argonne National Laboratory, and neutron diffraction at Oak Ridge National Laboratory). The objectives and accomplishments are summarized.

  12. Living SafeLy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    as safe as the electrical wiring in our homes - or just as danger- ous. The key is learning to act safely around them. This booklet is a basic safety guide for those who live...

  13. Effects of remediation amendments on vadose zone microorganisms

    SciTech Connect (OSTI)

    Miller, Hannah M.; Tilton, Fred A.

    2012-08-10

    Surfactant-based foam delivery technology has been studied to remediate Hanford 200 area deep vadose zone sediment. However, the surfactants and remediation amendments have an unknown effect on indigenous subsurface microorganisms. Microbial populations are important factors to consider in remediation efforts due to their potential to alter soil geochemistry. This project focuses on measuring microbial metabolic responses to remediation amendments in batch and column studies using Deep Vadose Zone Sediments. Initial studies of the microbes from Hanford 200 area deep vadose zone sediment showed surfactants sodium dodecyl sulfate (SDS) and cocamidopropyl betaine (CAPB) and remediation amendment calcium polysulfide (CPS) had no affect on microbial growth using BiologTM Ecoplates. To move towards a more realistic field analog, soil columns were packed with Hanford 200 Area sediment. Once microbial growth in the column was verified by observing growth of the effluent solution on tryptic soy agar plates, remedial surfactants were injected into the columns, and the resulting metabolic diversity was measured. Results suggest surfactant sodium dodecyl sulfate (SDS) stimulates microbial growth. The soil columns were also visualized using X-ray microtomography to inspect soil packing and possibly probe for evidence of biofilms. Overall, BiologTM Ecoplates provide a rapid assay to predict effects of remediation amendments on Hanford 200 area deep vadose zone microorganisms.

  14. Methods for identifying an essential gene in a prokaryotic microorganism

    DOE Patents [OSTI]

    Shizuya, Hiroaki

    2006-01-31

    Methods are provided for the rapid identification of essential or conditionally essential DNA segments in any species of haploid cell (one copy chromosome per cell) that is capable of being transformed by artificial means and is capable of undergoing DNA recombination. This system offers an enhanced means of identifying essential function genes in diploid pathogens, such as gram-negative and gram-positive bacteria.

  15. Organic acid-tolerant microorganisms and uses thereof for producing organic acids

    DOE Patents [OSTI]

    Pfleger, Brian Frederick; Begemann, Matthew Brett

    2014-05-06

    Organic acid-tolerant microorganisms and methods of using same. The organic acid-tolerant microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid (3HP), acrylic acid, and propionic acid. Further modifications to the microorganisms such as increasing expression of malonyl-CoA reductase and/or acetyl-CoA carboxylase provide or increase the ability of the microorganisms to produce 3HP. Methods of generating an organic acid with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers include replacing acsA or homologs thereof in cells with genes of interest and selecting for the cells comprising the genes of interest with amounts of organic acids effective to inhibit growth of cells harboring acsA or the homologs.

  16. Sensory Transduction in Microorganisms 2008 Gordon Research Conference (January 2008)

    SciTech Connect (OSTI)

    Ann M. Stock

    2009-04-08

    Research into the mechanisms involved in the sensing and responses of microorganisms to changes in their environments is currently very active in a large number of laboratories worldwide. An increasingly wide range of prokaryotic and eukaryotic species are being studied with regard to their sensing of diverse chemical and physical stimuli, including nutrients, toxins, intercellular signaling molecules, redox indicators, light, pressure, magnetic fields, and surface contact, leading to adaptive responses affecting motile behavior, gene expression and/or development. The ease of manipulation of microorganisms has facilitated application of a broad range of techniques that have provided comprehensive descriptions of cellular behavior and its underlying molecular mechanisms. Systems and their molecular components have been probed at levels ranging from the whole organism down to atomic resolution using behavioral analyses; electrophysiology; genetics; molecular biology; biochemical and biophysical characterization; structural biology; single molecule, fluorescence and cryo-electron microscopy; computational modeling; bioinformatics and genomic analyses. Several model systems such as bacterial chemotaxis and motility, fruiting body formation in Myxococcus xanthus, and motility and development in Dictyostelium discoideum have traditionally been a focus of this meeting. By providing a basis for assessment of similarities and differences in mechanisms, understanding of these pathways has advanced the study of many other microbial sensing systems. This conference aims to bring together researchers investigating different prokaryotic and eukaryotic microbial systems using diverse approaches to compare data, share methodologies and ideas, and seek to understand the fundamental principles underlying sensory responses. Topic areas include: (1) Receptor Sensing and Signaling; (2) Intracellular Signaling (two-component, c-di-GMP, c-AMP, etc.); (3) Intracellular Localization and the Cytoskeleton; (4) Motors and Motility; (5) Differentiation and Development; (6) Host/Pathogen and Host/Symbiont Interactions; (7) Intercellular Communication; (8) Microbes and the Environment; and (9) Modeling Signaling Pathways.

  17. Functionalized Polyacrylamide Monolith for Rapid Bacteria Pathogen...

    Office of Scientific and Technical Information (OSTI)

    Polyacrylamide Monolith for Rapid Bacteria Pathogen Detection in Human Blood. ... Sponsoring Org: USDOE National Nuclear Security Administration (NNSA) Country of ...

  18. Re-engineering bacteria for ethanol production

    DOE Patents [OSTI]

    Yomano, Lorraine P; York, Sean W; Zhou, Shengde; Shanmugam, Keelnatham; Ingram, Lonnie O

    2014-05-06

    The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.

  19. Engineering Biofuels from Photosynthetic Bacteria | Argonne National

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laboratory Engineering Biofuels from Photosynthetic Bacteria Technology available for licensing: Using photosynthetic bacteria to produce biofuels. 30-70% of the fuel's waste can be used to create other fuel sources Combines both engineered and natural photosynthetic mechanisms to generate the fuel PDF icon biofuels_from_bacteria

  20. Apparatus and method for transforming living cells

    DOE Patents [OSTI]

    Okandan, Murat; Galambos, Paul C.

    2003-11-11

    An apparatus and method are disclosed for in vitro transformation of living cells. The apparatus, which is formed as a microelectromechanical device by surface micromachining, can be used to temporarily disrupt the cell walls or membrane of host cells one at a time so that a particular substance (e.g. a molecular tag, nucleic acid, bacteria, virus etc.) can be introduced into the cell. Disruption of the integrity of the host cells (i.e. poration) can be performed mechanically or electrically, or by both while the host cells are contained within a flow channel. Mechanical poration is possible using a moveable member which has a pointed or serrated edge and which is driven by an electrostatic actuator to abrade, impact or penetrate the host cell. Electroporation is produced by generating a relatively high electric field across the host cell when the host cell is located in the flow channel between a pair of electrodes having a voltage applied therebetween.

  1. Engineering and Coordination of Regulatory Networks and Intracellular Complexes to Maximize Hydrogen Production by Phototrophic Microorganisms

    SciTech Connect (OSTI)

    James C. Liao

    2012-05-22

    This project is a collaboration with F. R. Tabita of Ohio State. Our major goal is to understand the factors and regulatory mechanisms that influence hydrogen production. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Our part of the project was to develop a modeling technique to investigate the metabolic network in connection to hydrogen production and regulation. Organisms must balance the pathways that generate and consume reducing power in order to maintain redox homeostasis to achieve growth. Maintaining this homeostasis in the nonsulfur purple photosynthetic bacteria is a complex feat with many avenues that can lead to balance, as these organisms possess versatile metabolic capabilities including anoxygenic photosynthesis, aerobic or anaerobic respiration, and fermentation. Growth is achieved by using H{sub 2} as an electron donor and CO{sub 2} as a carbon source during photoautotrophic and chemoautotrophic growth, where CO{sub 2} is fixed via the Calvin-Benson-Bassham (CBB) cycle. Photoheterotrophic growth can also occur when alternative organic carbon compounds are utilized as both the carbon source and electron donor. Regardless of the growth mode, excess reducing equivalents generated as a result of oxidative processes, must be transferred to terminal electron acceptors, thus insuring that redox homeostasis is maintained in the cell. Possible terminal acceptors include O{sub 2}, CO{sub 2}, organic carbon, or various oxyanions. Cells possess regulatory mechanisms to balance the activity of the pathways which supply energy, such as photosynthesis, and those that consume energy, such as CO{sub 2} assimilation or N{sub 2} fixation. The major route for CO{sub 2} assimilation is the CBB reductive pentose phosphate pathway, whose key enzyme is ribulose 1,5-biphosphate carboxylase/oxygenase (RubisCO). In addition to providing virtually all cellular carbon during autotrophic metabolism, RubisCO-mediated CO{sub 2} assimilation is also very important for nonsulfur purple photosynthetic bacteria under photoheterotrophic growth conditions since CO{sub 2} becomes the major electron sink under these conditions. In this work, Ensemble Modeling (EM) was developed to examine the behavior of CBB-compromised RubisCO knockout mutant strains of the nonsulfur purple photosynthetic bacterium Rhodobacter sphaeroides. Mathematical models of metabolism can be a great aid in studying the effects of large perturbations to the system, such as the inactivation of RubisCO. Due to the complex and highly-interconnected nature of these networks, it is not a trivial process to understand what the effect of perturbations to the metabolic network will be, or vice versa, what enzymatic perturbations are necessary to yield a desired effect. Flux distribution is controlled by multiple enzymes in the network, often indirectly linked to the pathways of interest. Further, depending on the state of the cell and the environmental conditions, the effect of a perturbation may center around how it effects the carbon flow in the network, the balancing of cofactors, or both. Thus, it is desirable to develop mathematical models to describe, understand, and predict network behavior. Through the development of such models, one may gain the ability to generate a set of testable hypotheses for system behavior.

  2. Correlative Electron and Fluorescence Microscopy of Magnetotactic Bacteria in Liquid: Toward In Vivo Imaging

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Woehl, Taylor J.; Kashyap, Sanjay; Firlar, Emre; Perez-Gonzalez, Teresa; Faivre, Damien; Trubitsyn, Denis; Bazylinski, Dennis A.; Prozorov, Tanya

    2014-10-31

    Magnetotactic bacteria biomineralize ordered chains of uniform, membrane-bound magnetite or greigite nanocrystals that exhibit nearly perfect crystal structures and species-specific morphologies. Transmission electron microscopy (TEM) is a critical technique for providing information regarding the organization of cellular and magnetite structures in these microorganisms. However, conventional TEM can only be used to image air-dried or vitrified bacteria removed from their natural environment. Here we present a correlative scanning TEM (STEM) and fluorescence microscopy technique for imaging viable cells of Magnetospirillum magneticum strain AMB-1 in liquid using an in situ fluid cell TEM holder. Fluorescently labeled cells were immobilized on microchip windowmore » surfaces and visualized in a fluid cell with STEM, followed by correlative fluorescence imaging to verify their membrane integrity. Notably, the post-STEM fluorescence imaging indicated that the bacterial cell wall membrane did not sustain radiation damage during STEM imaging at low electron dose conditions. We investigated the effects of radiation damage and sample preparation on the bacteria viability and found that approximately 50% of the bacterial membranes remained intact after an hour in the fluid cell, decreasing to ~30% after two hours. These results represent a first step toward in vivo studies of magnetite biomineralization in magnetotactic bacteria.« less

  3. Correlative Electron and Fluorescence Microscopy of Magnetotactic Bacteria in Liquid: Toward In Vivo Imaging

    SciTech Connect (OSTI)

    Woehl, Taylor J.; Kashyap, Sanjay; Firlar, Emre; Perez-Gonzalez, Teresa; Faivre, Damien; Trubitsyn, Denis; Bazylinski, Dennis A.; Prozorov, Tanya

    2014-10-31

    Magnetotactic bacteria biomineralize ordered chains of uniform, membrane-bound magnetite or greigite nanocrystals that exhibit nearly perfect crystal structures and species-specific morphologies. Transmission electron microscopy (TEM) is a critical technique for providing information regarding the organization of cellular and magnetite structures in these microorganisms. However, conventional TEM can only be used to image air-dried or vitrified bacteria removed from their natural environment. Here we present a correlative scanning TEM (STEM) and fluorescence microscopy technique for imaging viable cells of Magnetospirillum magneticum strain AMB-1 in liquid using an in situ fluid cell TEM holder. Fluorescently labeled cells were immobilized on microchip window surfaces and visualized in a fluid cell with STEM, followed by correlative fluorescence imaging to verify their membrane integrity. Notably, the post-STEM fluorescence imaging indicated that the bacterial cell wall membrane did not sustain radiation damage during STEM imaging at low electron dose conditions. We investigated the effects of radiation damage and sample preparation on the bacteria viability and found that approximately 50% of the bacterial membranes remained intact after an hour in the fluid cell, decreasing to ~30% after two hours. These results represent a first step toward in vivo studies of magnetite biomineralization in magnetotactic bacteria.

  4. Living olefin polymerization processes

    DOE Patents [OSTI]

    Schrock, R.R.; Baumann, R.

    1999-03-30

    Processes for the living polymerization of olefin monomers with terminal carbon-carbon double bonds are disclosed. The processes employ initiators that include a metal atom and a ligand having two group 15 atoms and a group 16 atom or three group 15 atoms. The ligand is bonded to the metal atom through two anionic or covalent bonds and a dative bond. The initiators are particularly stable under reaction conditions in the absence of olefin monomer. The processes provide polymers having low polydispersities, especially block copolymers having low polydispersities. It is an additional advantage of these processes that, during block copolymer synthesis, a relatively small amount of homopolymer is formed.

  5. Fuel Cells Go Live

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    green h y d r o g e n f u e l i n g POWer Fuel Cells Go live A closer look at the requirements to create a hydrogen-based warehouse M anagers of distribution centers are always on the lookout for new ways to gain competitive advantage through increased operational efficiency, productivity and worker safety. Around North America, some are finding success by integrating commercially available hydrogen fuel cell systems into their lift truck fleets. For operations with large fleets of electric lift

  6. Living olefin polymerization processes

    DOE Patents [OSTI]

    Schrock, Richard R.; Baumann, Robert

    2003-08-26

    Processes for the living polymerization of olefin monomers with terminal carbon-carbon double bonds are disclosed. The processes employ initiators that include a metal atom and a ligand having two group 15 atoms and a group 16 atom or three group 15 atoms. The ligand is bonded to the metal atom through two anionic or covalent bonds and a dative bond. The initiators are particularly stable under reaction conditions in the absence of olefin monomer. The processes provide polymers having low polydispersities, especially block copolymers having low polydispersities. It is an additional advantage of these processes that, during block copolymer synthesis, a relatively small amount of homopolymer is formed.

  7. Living olefin polymerization processes

    DOE Patents [OSTI]

    Schrock, Richard R. (Winchester, MA); Baumann, Robert (Cambridge, MA)

    1999-01-01

    Processes for the living polymerization of olefin monomers with terminal carbon-carbon double bonds are disclosed. The processes employ initiators that include a metal atom and a ligand having two group 15 atoms and a group 16 atom or three group 15 atoms. The ligand is bonded to the metal atom through two anionic or covalent bonds and a dative bond. The initiators are particularly stable under reaction conditions in the absence of olefin monomer. The processes provide polymers having low polydispersities, especially block copolymers having low polydispersities. It is an additional advantage of these processes that, during block copolymer synthesis, a relatively small amount of homopolymer is formed.

  8. Living olefin polymerization processes

    DOE Patents [OSTI]

    Schrock, Richard R.; Bauman, Robert

    2006-11-14

    Processes for the living polymerization of olefin monomers with terminal carbon-carbon double bonds are disclosed. The processes employ initiators that include a metal atom and a ligand having two group 15 atoms and a group 16 atom or three group 15 atoms. The ligand is bonded to the metal atom through two anionic or covalent bonds and a dative bond. The initiators are particularly stable under reaction conditions in the absence of olefin monomer. The processes provide polymers having low polydispersities, especially block copolymers having low polydispersities. It is an additional advantage of these processes that, during block copolymer synthesis, a relatively small amount of homopolymer is formed.

  9. Final Technical Report: Viral Infection of Subsurface Microorganisms and Metal/Radionuclide Transport

    SciTech Connect (OSTI)

    Weber, Karrie A.; Bender, Kelly S.; Li, Yusong

    2013-09-28

    Microbially mediated metabolisms have been identified as a significant factor either directly or indirectly impacting the fate and transport of heavy metal/radionuclide contaminants. To date microorganisms have been isolated from contaminated environments. Examination of annotated finished genome sequences of many of these subsurface isolates from DOE sites, revealed evidence of prior viral infection. To date the role that viruses play influencing microbial mortality and the resulting community structure which directly influences biogeochemical cycling in soils and sedimentary environments remains poorly understood. The objective of this exploratory study was to investigate the role of viral infection of subsurface bacteria and the formation of contaminant-bearing viral particles. This objective was approached by examining the following working hypotheses: (i) subsurface microorganisms are susceptible to viral infections by the indigenous subsurface viral community, and (ii) viral surfaces will adsorb heavy metals and radionuclides. Our results have addressed basic research needed to accomplish the BER Long Term Measure to provide sufficient scientific understanding such that DOE sites would be able to incorporate coupled physical, chemical and biological processes into decision making for environmental remediation or natural attenuation and long-term stewardship by establishing viral-microbial relationships on the subsequent fate and transport of heavy metals and radionuclides. Here we demonstrated that viruses play a significant role in microbial mortality and community structure in terrestrial subsurface sedimentary systems. The production of viral-like particles within subsurface sediments in response to biostimulation with dissolved organic carbon and a terminal electron acceptor resulted in the production of viral-like particles. Organic carbon alone did not result in significant viral production and required the addition of a terminal electron acceptor (nitrate), indicating that nutrients are not limiting viral production, but rather substrates that can be converted into energy for host metabolism. Our results also revealed that cell abundance was not correlated to the mineralization of organic carbon, but rather viruses were positively correlated with carbon mineralization. This is a result of viral-mediated cell lysis and demonstrates that viruses are sensitive indicators of microbial activity. Viruses as an indicator of microbial activity was not unique to batch culture studies as results obtained from an in situ field experiment conducted at the DOE Old Rifle Field site. This study revealed that viral abundance increased in response to the injection of oxygenated groundwater and influx of dissolved organic carbon whereas cell abundance changes were minimal. However, the extent to which viral-mediated cell lysis alters organic matter pools subsequently influencing microbial community structure and biogeochemical function remains a critical question in subsurface biogeochemical cycling. The production of significant numbers of viruses in groundwater has implications for nanoparticulate metal as well as carbon transport in groundwater. We have demonstrated that the virus surface is reactive and will adsorb heavy metals. Thus viruses can promote colloidal contaminant mobility. Interestingly, the presence of heavy metals has a positive effect on infectivity of the phage, increasing phage infection which could lead to further production of viruses. Together, the results indicate that the sorption of metals to the surface of viruses could not only contribute to nanoparticulate metal as well as carbon transport but could also enhance infectivity further contributing to cell lysis which could subsequently influence biogeochemical cycling. As more viruses infect host microbial populations the high concentration of metals would enhance infection, resulting in cell lysis, and decreasing the metabolically active host population while yielding greater numbers of viruses capable of transporting contaminats. Additional studie

  10. Transformation of gram positive bacteria by sonoporation

    DOE Patents [OSTI]

    Yang, Yunfeng; Li, Yongchao

    2014-03-11

    The present invention provides a sonoporation-based method that can be universally applied for delivery of compounds into Gram positive bacteria. Gram positive bacteria which can be transformed by sonoporation include, for example, Bacillus, Streptococcus, Acetobacterium, and Clostridium. Compounds which can be delivered into Gram positive bacteria via sonoporation include nucleic acids (DNA or RNA), proteins, lipids, carbohydrates, viruses, small organic and inorganic molecules, and nano-particles.

  11. Spectroscopic diagnostics for bacteria in biologic sample

    DOE Patents [OSTI]

    El-Sayed, Mostafa A. (Atlanta, GA); El-Sayed, Ivan H. (Somerville, MA)

    2002-01-01

    A method to analyze and diagnose specific bacteria in a biologic sample using spectroscopy is disclosed. The method includes obtaining the spectra of a biologic sample of a non-infected patient for use as a reference, subtracting the reference from the spectra of an infected sample, and comparing the fingerprint regions of the resulting differential spectrum with reference spectra of bacteria in saline. Using this diagnostic technique, specific bacteria can be identified sooner and without culturing, bacteria-specific antibiotics can be prescribed sooner, resulting in decreased likelihood of antibiotic resistance and an overall reduction of medical costs.

  12. Method of dispersing a hydrocarbon using bacteria

    DOE Patents [OSTI]

    Tyndall, R.L.

    1996-09-24

    A new protozoan derived microbial consortia and method for their isolation are provided. The isolated consortia and bacteria are useful for treating wastes such as trichloroethylene and trinitrotoluene. The isolated consortia, bacteria, and dispersants are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  13. Method of dispersing a hydrocarbon using bacteria

    DOE Patents [OSTI]

    Tyndall, Richard L. (Clinton, TN)

    1996-01-01

    New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

  14. Nucleic acid molecules conferring enhanced ethanol tolerance and microorganisms having enhanced tolerance to ethanol

    DOE Patents [OSTI]

    Brown, Steven; Guss, Adam; Yang, Shihui; Karpinets, Tatiana; Lynd, Lee; Shao, Xiongjun

    2014-01-14

    The present invention provides isolated nucleic acid molecules which encode a mutant acetaldehyde-CoA/alcohol dehydrogenase or mutant alcohol dehydrogenase and confer enhanced tolerance to ethanol. The invention also provides related expression vectors, genetically engineered microorganisms having enhanced tolerance to ethanol, as well as methods of making and using such genetically modified microorganisms for production of biofuels based on fermentation of biomass materials.

  15. Synthetic Design Microorganisms for Lignin Fuels and Chemicals Presentation for BETO 2015 Project Peer Review

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Synthetic Design Microorganisms for Lignin Fuels and Chemicals 3/26/2015 Synthetic Biology Joshua S. Yuan Associate Professor and Director Texas A&M University This presentation does contain proprietary information 1 Project Goal: Design of Microorganisms for Lignin Fuel * The proposed research aims to address one of the most challenging issues in biofuel production: the utilization of lignin for fungible fuels. * Project Outcome: A viable biological platform for conversion of lignin into

  16. Chemotactic selection of pollutant degrading soil bacteria

    DOE Patents [OSTI]

    Hazen, T.C.

    1991-03-04

    A method is described for identifying soil microbial strains which may be bacterial degraders of pollutants. This method includes: Placing a concentration of a pollutant in a substantially closed container; placing the container in a sample of soil for a period of time ranging from one minute to several hours; retrieving the container and collecting its contents; microscopically determining the identity of the bacteria present. Different concentrations of the pollutant can be used to determine which bacteria respond to each concentration. The method can be used for characterizing a polluted site or for looking for naturally occurring biological degraders of the pollutant. Then bacteria identified as degraders of the pollutant and as chemotactically attracted to the pollutant are used to innoculate contaminated soil. To enhance the effect of the bacteria on the pollutant, nutrients are cyclicly provided to the bacteria then withheld to alternately build up the size of the bacterial colony or community and then allow it to degrade the pollutant.

  17. Metabolic Engineering and Modeling of Metabolic Pathways to Improve Hydrogen Production by Photosynthetic Bacteria

    SciTech Connect (OSTI)

    Jiao, Y.; Navid, A.

    2014-12-19

    Rising energy demands and the imperative to reduce carbon dioxide (CO2) emissions are driving research on biofuels development. Hydrogen gas (H2) is one of the most promising biofuels and is seen as a future energy carrier by virtue of the fact that 1) it is renewable, 2) does not evolve the greenhouse gas CO2 in combustion, 3) liberates large amounts of energy per unit weight in combustion (having about 3 times the energy content of gasoline), and 4) is easily converted to electricity by fuel cells. Among the various bioenergy strategies, environmental groups and others say that the concept of the direct manufacture of alternative fuels, such as H2, by photosynthetic organisms is the only biofuel alternative without significant negative criticism [1]. Biological H2 production by photosynthetic microorganisms requires the use of a simple solar reactor such as a transparent closed box, with low energy requirements, and is considered as an attractive system to develop as a biocatalyst for H2 production [2]. Various purple bacteria including Rhodopseudomonas palustris, can utilize organic substrates as electron donors to produce H2 at the expense of solar energy. Because of the elimination of energy cost used for H2O oxidation and the prevention of the production of O2 that inhibits the H2-producing enzymes, the efficiency of light energy conversion to H2 by anoxygenic photosynthetic bacteria is in principle much higher than that by green algae or cyanobacteria, and is regarded as one of the most promising cultures for biological H2 production [3]. Here implemented a simple and relatively straightforward strategy for hydrogen production by photosynthetic microorganisms using sunlight, sulfur- or iron-based inorganic substrates, and CO2 as the feedstock. Carefully selected microorganisms with bioengineered beneficial traits act as the biocatalysts of the process designed to both enhance the system efficiency of CO2 fixation and the net hydrogen production rate. Additionally we applied metabolic engineering approaches guided by computational modeling for the chosen model microorganisms to enable efficient hydrogen production.

  18. Methane and Methanotrophic Bacteria as a Biotechnological Platform...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Methane and Methanotrophic Bacteria as a Biotechnological Platform Methane and Methanotrophic Bacteria as a Biotechnological Platform Breakout Session 2-B: NewEmerging Pathways...

  19. Comparative genomics of the lactic acid bacteria

    SciTech Connect (OSTI)

    Makarova, K.; Slesarev, A.; Wolf, Y.; Sorokin, A.; Mirkin, B.; Koonin, E.; Pavlov, A.; Pavlova, N.; Karamychev, V.; Polouchine, N.; Shakhova, V.; Grigoriev, I.; Lou, Y.; Rokhsar, D.; Lucas, S.; Huang, K.; Goodstein, D. M.; Hawkins, T.; Plengvidhya, V.; Welker, D.; Hughes, J.; Goh, Y.; Benson, A.; Baldwin, K.; Lee, J.-H.; Diaz-Muniz, I.; Dosti, B.; Smeianov, V,; Wechter, W.; Barabote, R.; Lorca, G.; Altermann, E.; Barrangou, R.; Ganesan, B.; Xie, Y.; Rawsthorne, H.; Tamir, D.; Parker, C.; Breidt, F.; Broadbent, J.; Hutkins, R.; O'Sullivan, D.; Steele, J.; Unlu, G.; Saier, M.; Klaenhammer, T.; Richardson, P.; Kozyavkin, S.; Weimer, B.; Mills, D.

    2006-06-01

    Lactic acid-producing bacteria are associated with various plant and animal niches and play a key role in the production of fermented foods and beverages. We report nine genome sequences representing the phylogenetic and functional diversity of these bacteria. The small genomes of lactic acid bacteria encode a broad repertoire of transporters for efficient carbon and nitrogen acquisition from the nutritionally rich environments they inhabit and reflect a limited range of biosynthetic capabilities that indicate both prototrophic and auxotrophic strains. Phylogenetic analyses, comparison of gene content across the group, and reconstruction of ancestral gene sets indicate a combination of extensive gene loss and key gene acquisitions via horizontal gene transfer during the coevolution of lactic acid bacteria with their habitats.

  20. How Bacteria Make Magnets | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    How Bacteria Make Magnets For a number of animals, including birds, fish and mammals, there is evidence that magnets are used for orientation. However, little is known about how...

  1. Methods for dispersing hydrocarbons using autoclaved bacteria

    DOE Patents [OSTI]

    Tyndall, Richard L. (Clinton, TN)

    1996-01-01

    A method of dispersing a hydrocarbon includes the steps: providing a bacterium selected from the following group: ATCC 85527, ATCC 75529, and ATCC 55638, a mutant of any one of these bacteria possessing all the identifying characteristics of any one of these bacteria, and mixtures thereof; autoclaving the bacterium to derive a dispersant solution therefrom; and contacting the dispersant solution with a hydrocarbon to disperse the hydrocarbon. Moreover, a method for preparing a dispersant solution includes the following steps: providing a bacterium selected from the following group: ATCC 75527, ATCC 75529, and ATCC 55638, a mutant of any one of these bacteria possessing all the identifying characteristics of any one of these bacteria, and mixtures thereof; and autoclaving the bacterium to derive a dispersant solution therefrom.

  2. Methods for dispersing hydrocarbons using autoclaved bacteria

    DOE Patents [OSTI]

    Tyndall, R.L.

    1996-11-26

    A method of dispersing a hydrocarbon includes the following steps: providing a bacterium selected from the following group: ATCC 85527, ATCC 75529, and ATCC 55638, a mutant of any one of these bacteria possessing all the identifying characteristics of any one of these bacteria, and mixtures; autoclaving the bacterium to derive a dispersant solution; and contacting the dispersant solution with a hydrocarbon to disperse the hydrocarbon. Moreover, a method for preparing a dispersant solution includes the following steps: providing a bacterium selected from the following group: ATCC 75527, ATCC 75529, and ATCC 55638, a mutant of any one of these bacteria possessing all the identifying characteristics of any one of these bacteria, and mixtures; and autoclaving the bacterium to derive a dispersant solution.

  3. Functionalized Polyacrylamide Monolith for Rapid Bacteria Pathogen

    Office of Scientific and Technical Information (OSTI)

    Detection in Human Blood. (Conference) | SciTech Connect Conference: Functionalized Polyacrylamide Monolith for Rapid Bacteria Pathogen Detection in Human Blood. Citation Details In-Document Search Title: Functionalized Polyacrylamide Monolith for Rapid Bacteria Pathogen Detection in Human Blood. Abstract not provided. Authors: Mai, Junyu ; Piccini, Matthew Ernest ; Hatch, Anson V. ; Abhyankar, Vinay V. ; Olano, Juan ; Willson, Richard Publication Date: 2014-01-01 OSTI Identifier: 1140705

  4. Fuel from Bacteria: Bioconversion of Carbon Dioxide to Biofuels by Facultatively Autotrophic Hydrogen Bacteria

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: Ohio State is genetically modifying bacteria to efficiently convert carbon dioxide directly into butanol, an alcohol that can be used directly as a fuel blend or converted to a hydrocarbon, which closely resembles a gasoline. Bacteria are typically capable of producing a certain amount of butanol before it becomes too toxic for the bacteria to survive. Ohio State is engineering a new strain of the bacteria that could produce up to 50% more butanol before it becomes too toxic for the bacteria to survive. Finding a way to produce more butanol more efficiently would significantly cut down on biofuel production costs and help make butanol cost competitive with gasoline. Ohio State is also engineering large tanks, or bioreactors, to grow the biofuel-producing bacteria in, and they are developing ways to efficiently recover biofuel from the tanks.

  5. Phase Preference by Active, Acetate-Utilizing Bacteria at the Rifle, CO Integrated Field Research Challenge Site

    SciTech Connect (OSTI)

    Kerkhoff, Lee; Williams, Kenneth H.; Long, Philip E.; McGuinness, L.

    2011-02-15

    Uranium contaminated groundwaters are a legacy concern for the U.S. Department of Energy. Previous experiments at the Rifle, Colorado Integrated Field Challenge (IFC) site have demonstrated that field-scale addition of acetate to groundwater reduces the ambient soluable uranium concentration, sequestering the radionuclide as uraninite. However, questions remain regarding which microorganism(s) are consuming this acetate and if active groundwater microorganisms are different from active particle-associated bacteria. In this report, 13-C acetate was used to assess the active microbes that synthesize DNA on 3 size fractions [coarse sand, fines (8-approximately 150 micron), groundwater (0.2-8 micron)] over a 24 -day time frame. Results indicated a stronger signal from 13-C acetate associated with the fines fraction compared with smaller amounts of 13-C uptake on the sand fraction and groundwater samples during the SIP incubations. TRFLP analysis of this 13-C-labeled DNA, indicated 31+ 9 OTU's with 6 peaks dominating the active profiles (166, 187, 210, 212, and 277 bp peaks using MnlI). Cloning/sequencing of the amplification products indicated a Geobacter-like group (187, 210, 212 bp) primarily synthesized DNA from acetate in the groundwater phase, an alpha Proteobacterium (166 bp) primarily grew on the fines/sands, and an Acinetobacter sp. (277 bp) utilized much of the 13C acetate in both groundwater and particle-associated phases. These findings will help to delineate the acetate utilization patterns of bacteria during field-scale acetate addition and can lead to improved methods for stimulating distinct microbial populations in situ.

  6. Chemotactic selection of pollutant degrading soil bacteria

    DOE Patents [OSTI]

    Hazen, Terry C. (Augusta, GA)

    1994-01-01

    A method for identifying soil microbial strains which may be bacterial degraders of pollutants comprising the steps of placing a concentration of a pollutant in a substantially closed container, placing the container in a sample of soil for a period of time ranging from one minute to several hours, retrieving the container, collecting the contents of the container, and microscopically determining the identity of the bacteria present. Different concentrations of the pollutant can be used to determine which bacteria respond to each concentration. The method can be used for characterizing a polluted site or for looking for naturally occurring biological degraders of the pollutant. Then bacteria identified as degraders of the pollutant and as chemotactically attracted to the pollutant are used to inoculate contaminated soil. To enhance the effect of the bacteria on the pollutant, nutrients are cyclicly provided to the bacteria then withheld to alternately build up the size of the bacterial colony or community and then allow it to degrade the pollutant.

  7. Living Villages Holdings Ltd | Open Energy Information

    Open Energy Info (EERE)

    Holdings Ltd Jump to: navigation, search Name: Living Villages Holdings Ltd Place: England, United Kingdom Zip: Shropshire SY9 Product: Living Villages is a residential property...

  8. Solar Living Institute | Open Energy Information

    Open Energy Info (EERE)

    Living Institute Jump to: navigation, search Name: Solar Living Institute Address: 13771 S. Hwy. 101 Place: Hopland, California Zip: 95449 Region: Bay Area Website:...

  9. Living Buildings Ltd | Open Energy Information

    Open Energy Info (EERE)

    Ltd Jump to: navigation, search Name: Living Buildings Ltd Place: United Kingdom Sector: Buildings Product: Living Buildings is an installer of small-scale distributed generation...

  10. Soft rot decay capabilities and interactions of fungi and bacteria from fumigated utility poles

    SciTech Connect (OSTI)

    Wang, C.J.K.; Worrall, J.J. . Coll. of Environmental Science and Forestry)

    1992-11-01

    The objectives were to (1) identify microfungi and bacterial associates isolated from fumigated southern pine poles from EPRI project RP 1471-72, (2) study the soft-rot capabilities of predominant fungi, and (3) study interactions among microorganisms in relation to wood decay. Methods for identification followed standard techniques using morphological and physiological criteria. Soft-rot by microfungi alone and with bacteria was determined as weight loss and anatomical examination of wood blocks using light microscopy and limited electron microscopy. Acinetobacter calcoaceticus was the predominant bacterium. Twenty-one species of microfungi were identified including four new species. A book entitled IDENTIFICATION MANUAL FOR FUNGI FROM UTILITY POLES IN THE EASTERN UNITED STATES was published. An improved soft-rot test was devised. Fifty-one of 84 species (60%) of microfungi from poles tested were soft-rot positive; that is much greater than previously reported. Three types of anatomical damage of wood of pine or birch caused by soft-rot fungi were described. Interaction tests showed that, in some cases, there was a strong synergism between bacteria and fungi in causing weight loss, but results were inconsistent. Although soft rot is often most apparent under conditions of very high moisture, intermediate moisture levels appear to be optimal, as with basidiomycete decayers.

  11. Soft rot decay capabilities and interactions of fungi and bacteria from fumigated utility poles. Final report

    SciTech Connect (OSTI)

    Wang, C.J.K.; Worrall, J.J.

    1992-11-01

    The objectives were to (1) identify microfungi and bacterial associates isolated from fumigated southern pine poles from EPRI project RP 1471-72, (2) study the soft-rot capabilities of predominant fungi, and (3) study interactions among microorganisms in relation to wood decay. Methods for identification followed standard techniques using morphological and physiological criteria. Soft-rot by microfungi alone and with bacteria was determined as weight loss and anatomical examination of wood blocks using light microscopy and limited electron microscopy. Acinetobacter calcoaceticus was the predominant bacterium. Twenty-one species of microfungi were identified including four new species. A book entitled IDENTIFICATION MANUAL FOR FUNGI FROM UTILITY POLES IN THE EASTERN UNITED STATES was published. An improved soft-rot test was devised. Fifty-one of 84 species (60%) of microfungi from poles tested were soft-rot positive; that is much greater than previously reported. Three types of anatomical damage of wood of pine or birch caused by soft-rot fungi were described. Interaction tests showed that, in some cases, there was a strong synergism between bacteria and fungi in causing weight loss, but results were inconsistent. Although soft rot is often most apparent under conditions of very high moisture, intermediate moisture levels appear to be optimal, as with basidiomycete decayers.

  12. Anaerobic metabolism of nitroaromatic compounds by sulfate-reducing and methanogenic bacteria

    SciTech Connect (OSTI)

    Boopathy, R.; Kulpa, C.F.

    1994-06-01

    Ecological observations suggest that sulfate-reducing and methanogenic bacteria might metabolize nitroaromatic compounds under anaerobic conditions if appropriate electron donors and electron acceptors are present in the environment, but this ability had not been demonstrated until recently. Most studies on the microbial metabolism of nitroaromatic compounds used aerobic microorganisms. In most cases no mineralization of nitroaromatics occurs, and only superficial modifications of the structures are reported. However, under anaerobic sulfate-reducing conditions, the nitroaromatic compounds reportedly undergo a series of reductions with the formation of amino compounds. For example, trinitrotoluene under sulfate-reducing conditions is reduced to triaminotoluene by the enzyme nitrite reductase, which is commonly found in many Desulfovibrio spp. The removal of ammonia from triaminotoluene is achieved by reductive deamination catalyzed by the enzyme reductive deaminase, with the production of ammonia and toluene. Some sulfate reducers can metabolize toluene to CO{sub 2}. Similar metabolic processes could be applied to other nitroaromatic compounds like nitrobenzene, nitrobenzoic acids, nitrophenols, and aniline. Many methanogenic bacteria can reduce nitroaromatic compounds to amino compounds. In this paper we review the anaerobic metabolic processes of nitroaromatic compounds under sulfate-reducing And methanogenic conditions.

  13. Biofuel from Bacteria and Sunlight: Shewanella as an Ideal Platform for Producing Hydrocarbons

    SciTech Connect (OSTI)

    None

    2010-01-01

    Broad Funding Opportunity Announcement Project: The University of Minnesota is developing clean-burning, liquid hydrocarbon fuels from bacteria. The University is finding ways to continuously harvest hydrocarbons from a type of bacteria called Shewanella by using a photosynthetic organism to constantly feed Shewanella the sugar it needs for energy and hydrocarbon production. The two organisms live and work together as a system. Using Shewanella to produce hydrocarbon fuels offers several advantages over traditional biofuel production methods. First, it eliminates many of the time-consuming and costly steps involved in growing plants and harvesting biomass. Second, hydrocarbon biofuels resemble current petroleum-based fuels and would therefore require few changes to the existing fuel refining and distribution infrastructure in the U.S.

  14. Natural Oil Production from Microorganisms: Bioprocess and Microbe Engineering for Total Carbon Utilization in Biofuel Production

    SciTech Connect (OSTI)

    2010-07-15

    Electrofuels Project: MIT is using carbon dioxide (CO2) and hydrogen generated from electricity to produce natural oils that can be upgraded to hydrocarbon fuels. MIT has designed a 2-stage biofuel production system. In the first stage, hydrogen and CO2 are fed to a microorganism capable of converting these feedstocks to a 2-carbon compound called acetate. In the second stage, acetate is delivered to a different microorganism that can use the acetate to grow and produce oil. The oil can be removed from the reactor tank and chemically converted to various hydrocarbons. The electricity for the process could be supplied from novel means currently in development, or more proven methods such as the combustion of municipal waste, which would also generate the required CO2 and enhance the overall efficiency of MITs biofuel-production system.

  15. Advanced Biofuels: How Scientists are Engineering Bacteria to Help Drive

    Energy Savers [EERE]

    America | Department of Energy Advanced Biofuels: How Scientists are Engineering Bacteria to Help Drive America Advanced Biofuels: How Scientists are Engineering Bacteria to Help Drive America December 6, 2011 - 2:12pm Addthis Strains of E. coli bacteria were engineered to digest switchgrass biomass and synthesize its sugars into gasoline, diesel and jet fuel. | Image courtesy of Berkeley Lab. Strains of E. coli bacteria were engineered to digest switchgrass biomass and synthesize its sugars

  16. Biochemistry and physiology of anaerobic bacteria

    SciTech Connect (OSTI)

    2000-05-18

    We welcome you to The Power of Anaerobes. This conference serves two purposes. One is to celebrate the life of Harry D. Peck, Jr.,who was born May 18, 1927 and would have celebrated his 73rd birthday at this conference. He died November 20, 1998. The second is to gather investigators to exchange views within the realm of anaerobic microbiology, an area in which tremendous progress has been seen during recent years. It is sufficient to mention discoveries of a new form of life (the archaea), hyper or extreme thermophiles, thermophilic alkaliphiles and anaerobic fungi. With these discoveries has come a new realization about physiological and metabolic properties of microorganisms, and this in turn has demonstrated their importance for the development, maintenance and sustenance of life on Earth.

  17. Living Direct: Order (2011-CE-1904)

    Office of Energy Efficiency and Renewable Energy (EERE)

    DOE ordered Living Direct, Inc. to pay a $6,000 civil penalty after finding Living Direct had failed to certify that certain models of dishwashers, refrigerator-freezers and freezers comply with the applicable energy conservation standards.

  18. ARM - General Changes in Daily Lives

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ListGeneral Changes in Daily Lives Outreach Home Room News Publications Traditional Knowledge Kiosks Barrow, Alaska Tropical Western Pacific Site Tours Contacts Students Study Hall About ARM Global Warming FAQ Just for Fun Meet our Friends Cool Sites Teachers Teachers' Toolbox Lesson Plans General Changes in Daily Lives Changes in our daily lives mainly depend on where we live and work. Building styles, working conditions, use of water, energy resources, crops, and leisure activities will need

  19. Live Fire Range Environmental Assessment

    SciTech Connect (OSTI)

    1993-08-01

    The Central Training Academy (CTA) is a DOE Headquarters Organization located in Albuquerque, New Mexico, with the mission to effectively and efficiently educate and train personnel involved in the protection of vital national security interests of DOE. The CTA Live Fire Range (LFR), where most of the firearms and tactical training occurs, is a complex separate from the main campus. The purpose of the proposed action is to expand the LFR to allow more options of implementing required training. The Department of Energy has prepared this Environmental Assessment (EA) for the proposed construction and operation of an expanded Live Fire Range Facility at the Central Training Academy in Albuquerque, New Mexico. Based on the analysis in the EA, DOE has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an environmental impact statement is not required and DOE is issuing this Finding of No Significant Impact (FONSI).

  20. Design and mechanism of action of a novel bacteria-selective antimicrobial peptide from the cell-penetrating peptide Pep-1

    SciTech Connect (OSTI)

    Zhu, W.L.; Lan Hongliang; Park, Il-Seon; Kim, Jae Il; Jin, H.Z.; Hahm, Kyung-Soo; Shin, S.Y. . E-mail: syshin@chosun.ac.kr

    2006-10-20

    Here, we report the successful design of a novel bacteria-selective antimicrobial peptide, Pep-1-K (KKTWWKTWWTKWSQPKKKRKV). Pep-1-K was designed by replacing Glu-2, Glu-6, and Glu-11 in the cell-penetrating peptide Pep-1 with Lys. Pep-1-K showed strong antibacterial activity against reference strains (MIC = 1-2 {mu}M) of Gram-positive and Gram-negative bacteria as well as against clinical isolates (MIC = 1-8 {mu}M) of methicillin-resistant Staphylococcus aureus and multidrug-resistant Pseudomonas aeruginosa. In contrast, Pep-1-K did not cause hemolysis of human erythrocytes even at 200 {mu}M. These results indicate that Pep-1-K may be a good candidate for antimicrobial drug development, especially as a topical agent against antibiotic-resistant microorganisms. Tryptophan fluorescence studies indicated that the lack of hemolytic activity of Pep-1-K correlated with its weak ability to penetrate zwitterionic phosphatidylcholine/cholesterol (10:1, w/w) vesicles, which mimic eukaryotic membranes. Furthermore, Pep-1-K caused little or no dye leakage from negatively charged phosphatidylethanolamine/phosphatidylglycerol (7:3, w/w) vesicles, which mimic bacterial membranes but had a potent ability to cause depolarization of the cytoplasmic membrane potential of intact S. aureus cells. These results suggested that Pep-1-K kills microorganisms by not the membrane-disrupting mode but the formation of small channels that permit transit of ions or protons but not molecules as large as calcein.

  1. International symposium on cellular and molecular biology of phosphate and phosphorylated compounds in microorganisms: Proceedings

    SciTech Connect (OSTI)

    1993-12-31

    This report contains the abstracts of papers presented at the conference. Attention is focused on the following topics: regulation of phosphate metabolism in bacteria; structure-function of alkaline phosphatase; regulation of phosphate metabolism in yeast; transport of phosphate and phosphorylated compounds; and phosphate regulation in pathogenesis and secondary metabolism.

  2. Detection of phenols using engineered bacteria

    DOE Patents [OSTI]

    Wise, Arlene A. (Philadelphia, PA); Kuske, Cheryl R. (Los Alamos, NM); Terwilliger, Thomas C. (Santa Fe, NM)

    2007-12-04

    Detection of phenols using engineered bacteria. A biosensor can be created by placing a reporter gene under control of an inducible promoter. The reporter gene produces a signal when a cognate transcriptional activator senses the inducing chemical. Creation of bacterial biosensors is currently restricted by limited knowledge of the genetic systems of bacteria that catabolize xenobiotics. By using mutagenic PCR to change the chemical specificity of the Pseudomonas species CF600 DmpR protein, the potential for engineering novel biosensors for detection of phenols has been demonstrated. DmpR, a well-characterized transcriptional activator of the P. CF600's dmp operon mediates growth on simple phenols. Transcription from Po, the promoter heading the dmp operon, is activated when the sensor domain of DmpR interacts with phenol and mono-substituted phenols. By altering the sensor domain of the DmpR, a group of DmpR derivatives that activate transcription of a Po-lacZ fusion in response to eight of the EPA's eleven priority pollutant phenols has been created. The assays and the sensor domain mutations that alter the chemical specificity of DmpR is described.

  3. Detection of phenols using engineered bacteria

    DOE Patents [OSTI]

    Wise, Arlene A.; Kuske, Cheryl R.; Terwilliger, Thomas C.

    2004-08-10

    Detection of phenols using engineered bacteria. A biosensor can be created by placing a reporter gene under control of an inducible promoter. The reporter gene produces a signal when a cognate transcriptional activator senses the inducing chemical. Creation of bacterial biosensors is currently restricted by limited knowledge of the genetic systems of bacteria that catabolize xenobiotics. By using mutagenic PCR to change the chemical specificity of the Pseudomonas species CF600 DmpR protein, the potential for engineering novel biosensors for detection of phenols has been demonstrated. DmpR, a well-characterized transcriptional activator of the P. CF600's dmp operon mediates growth on simple phenols. Transcription from Po, the promoter heading the dmp operon, is activated when the sensor domain of DmpR interacts with phenol and mono-substituted phenols. By altering the sensor domain of the DmpR, a group of DmpR derivatives that activate transcription of a Po-lacZ fusion in response to eight of the EPA's eleven priority pollutant phenols has been created. The assays and the sensor domain mutations that alter the chemical specificity of DmpR is described.

  4. Methane and Methanotrophic Bacteria as a Biotechnological Platform |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Methane and Methanotrophic Bacteria as a Biotechnological Platform Methane and Methanotrophic Bacteria as a Biotechnological Platform Breakout Session 2-B: New/Emerging Pathways Methane and Methanotrophic Bacteria as a Biotechnological Platform Dr. Lori Giver, Vice President of Biological Engineering, Calysta Energy, Inc. PDF icon giver_bioenergy_2015.pdf More Documents & Publications CX-100166 Categorical Exclusion Determination Biobased Chemicals Landscape in 2015:

  5. Argonne scientists use bacteria to power simple machines | Argonne...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    University and placed in the solution along with the common aerobic bacteria Bacillus subtilis. Andrey Sokolov of Princeton University and Igor Aronson from Argonne, along...

  6. Bacteria mix it up at the microscopic level | Argonne National...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Technology and now postdoctoral researcher at Princeton University, piled Bacillus subtillis bacteria into thin films to decode the physics that govern how they move....

  7. Rapid quantification of mutant fitness in diverse bacteria by...

    Office of Scientific and Technical Information (OSTI)

    Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons Citation Details In-Document Search Title: Rapid quantification of mutant...

  8. Rapid quantification of mutant fitness in diverse bacteria by...

    Office of Scientific and Technical Information (OSTI)

    Journal Article: Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons Citation Details In-Document Search Title: Rapid...

  9. Discovery of functional toxin/antitoxin systems in bacteria by...

    Office of Scientific and Technical Information (OSTI)

    Discovery of functional toxinantitoxin systems in bacteria by shotgun cloning Citation ... Research Org: Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US) ...

  10. Extending the Operating Lives of Materials

    ScienceCinema (OSTI)

    Paul Jablonski

    2010-09-01

    Metallurgist Paul Jablonski discusses his role in developing processes that extend the operating temperatures and operating lives of materials used in energy applications.

  11. Lancaster Live/Work Townhome Prototype

    SciTech Connect (OSTI)

    2010-04-01

    This case study describes development of a prototype live-work townhome that is highly efficient at 45% energy savings (95% counting photovoltaic system).

  12. Living Walls | OpenEI Community

    Open Energy Info (EERE)

    systems and the new field of biomimicry. Biomimicry is the science of imitating nature to solve human design problems. The Living Wall concept takes the principles behind...

  13. Live Broadcast on Quadrennial Energy Review

    Broader source: Energy.gov [DOE]

    Watch a live broadcast of the Quadrennial Energy Review public stakeholder meeting on Thursday, February 4, 2016 at 9:30 a.m. EST

  14. Rural Living Canada Website | Open Energy Information

    Open Energy Info (EERE)

    Equivalent URI: cleanenergysolutions.orgcontentrural-living-canada-website Language: English Policies: "Deployment Programs,Regulations" is not in the list of possible...

  15. Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity

    DOE Patents [OSTI]

    Premuzic, Eugene T. (East Moriches, NY); Lin, Mow (Rocky Point, NY)

    1996-02-20

    This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70.degree. C. to 90.degree. C., at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%.

  16. Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels

    SciTech Connect (OSTI)

    Kuk Lee, Sung; Chou, Howard; Ham, Timothy S.; Soon Lee, Taek; Keasling, Jay D.

    2009-12-02

    The ability to generate microorganisms that can produce biofuels similar to petroleum-based transportation fuels would allow the use of existing engines and infrastructure and would save an enormous amount of capital required for replacing the current infrastructure to accommodate biofuels that have properties significantly different from petroleum-based fuels. Several groups have demonstrated the feasibility of manipulating microbes to produce molecules similar to petroleum-derived products, albeit at relatively low productivity (e.g. maximum butanol production is around 20 g/L). For cost-effective production of biofuels, the fuel-producing hosts and pathways must be engineered and optimized. Advances in metabolic engineering and synthetic biology will provide new tools for metabolic engineers to better understand how to rewire the cell in order to create the desired phenotypes for the production of economically viable biofuels.

  17. Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity

    DOE Patents [OSTI]

    Premuzic, E.T.; Lin, M.

    1996-02-20

    This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70 C to 90 C, at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%. 68 figs.

  18. How sulphate-reducing microorganisms cope with stress: Lessons from systems biology

    SciTech Connect (OSTI)

    Zhou, J.; He, Q.; Hemme, C.L.; Mukhopadhyay, A.; Hillesland, K.; Zhou, A.; He, Z.; Nostrand, J.D. Van; Hazen, T.C.; Stahl, D.A.; Wall, J.D.; Arkin, A.P.

    2011-04-01

    Sulphate-reducing microorganisms (SRMs) are a phylogenetically diverse group of anaerobes encompassing distinct physiologies with a broad ecological distribution. As SRMs have important roles in the biogeochemical cycling of carbon, nitrogen, sulphur and various metals, an understanding of how these organisms respond to environmental stresses is of fundamental and practical importance. In this Review, we highlight recent applications of systems biology tools in studying the stress responses of SRMs, particularly Desulfovibrio spp., at the cell, population, community and ecosystem levels. The syntrophic lifestyle of SRMs is also discussed, with a focus on system-level analyses of adaptive mechanisms. Such information is important for understanding the microbiology of the global sulphur cycle and for developing biotechnological applications of SRMs for environmental remediation, energy production, biocorrosion control, wastewater treatment and mineral recovery.

  19. Connecticut Weatherization Project Improves Lives, Receives National

    Office of Environmental Management (EM)

    Recognition | Department of Energy Connecticut Weatherization Project Improves Lives, Receives National Recognition Connecticut Weatherization Project Improves Lives, Receives National Recognition May 6, 2014 - 12:24pm Addthis Donna Hawkins Technology Transfer Specialist, Weatherization Assistance Program Harris Walker Communications Specialist, Weatherization and Intergovernmental Program MORE WEATHERIZATION STORIES Improving Energy Efficiency and Creating Jobs through Weatherization

  20. Potential of Diazorphic, Endophytic Bacteria Associated with Sugarcane for

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energycane Production | Department of Energy Potential of Diazorphic, Endophytic Bacteria Associated with Sugarcane for Energycane Production Potential of Diazorphic, Endophytic Bacteria Associated with Sugarcane for Energycane Production This presentation by Michal Gisham was given at the Symbiosis Conference. PDF icon symbiosis_conference_grisham.pdf More Documents & Publications Symbiosis Biofeedstock Conference: Expanding Commercialization of Mutualistic Microbes to Increase

  1. Metal Cycling by Bacteria: Moving Electrons Around

    ScienceCinema (OSTI)

    Nealson, Ken

    2010-01-08

    About 20 years ago, Shewanella oneidensis MR-1 was isolated from a manganese-rich lack in upstate New York, and subsequently shown to utilize solid forms of oxidized manganese or iron as an electron acceptor. Recent studies of metal-reducing bacterial have unveiled a number of unexpected properties of microbes that have enlarged our view of microbes and their role(s) in natural ecosystems. For example, the processes of metal reduction themselves are fundamental to the carbon cycle in many lakes and sediments, where iron and manganese account for the major portion of organic carbon oxidation in many sediments. On more modest spatial scales, iron and manganese reduction can be linked to the oxidation of a wide variety of carbon compounds, many of them recalcitrant and/or toxic. One remarkable property of metal reducers is their ability to reduce solid, often highly crystalline substrates such as iron and manganese oxides and oxyhydroxides. It is now clear that this is done via the utilization of enzymes located on the outer wall of the bacteria - enzymes that apparently interact directly with these solid substrates. Molecular and genomic studies combined have revealed the genes and protoeins responsible for these activities, and many facets of the regulation. This talk focuses on the general features and properties of these remarkable organisms that seem to communicate via electron transfer across a wide variety of soluable, insoluable, and even "inert" substrates, and the way that these processes may be mechanistically linked.

  2. Metal Cycling by Bacteria: Moving Electrons Around

    SciTech Connect (OSTI)

    Nealson, Ken

    2009-07-06

    About 20 years ago, Shewanella oneidensis MR-1 was isolated from a manganese-rich lack in upstate New York, and subsequently shown to utilize solid forms of oxidized manganese or iron as an electron acceptor. Recent studies of metal-reducing bacterial have unveiled a number of unexpected properties of microbes that have enlarged our view of microbes and their role(s) in natural ecosystems. For example, the processes of metal reduction themselves are fundamental to the carbon cycle in many lakes and sediments, where iron and manganese account for the major portion of organic carbon oxidation in many sediments. On more modest spatial scales, iron and manganese reduction can be linked to the oxidation of a wide variety of carbon compounds, many of them recalcitrant and/or toxic. One remarkable property of metal reducers is their ability to reduce solid, often highly crystalline substrates such as iron and manganese oxides and oxyhydroxides. It is now clear that this is done via the utilization of enzymes located on the outer wall of the bacteria - enzymes that apparently interact directly with these solid substrates. Molecular and genomic studies combined have revealed the genes and protoeins responsible for these activities, and many facets of the regulation. This talk focuses on the general features and properties of these remarkable organisms that seem to communicate via electron transfer across a wide variety of soluable, insoluable, and even "inert" substrates, and the way that these processes may be mechanistically linked.

  3. Identification of bacteria synthesizing ribosomal RNA in response to uranium addition during biostimulation at the Rifle, CO Integrated Field Research site

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    McGuinness, Lora R.; Wilkins, Michael J.; Williams, Kenneth H.; Long, Philip E.; Kerkhof, Lee J.; Boyanov, Maxim I.

    2015-09-18

    Understanding which organisms are capable of reducing uranium at historically contaminated sites provides crucial information needed to evaluate treatment options and outcomes. One approach is determination of the bacteria which directly respond to uranium addition. In this research, uranium amendments were made to groundwater samples from a site of ongoing biostimulation with acetate. The active microbes in the planktonic phase were deduced by monitoring ribosomes production via RT-PCR. The results indicated several microorganisms were synthesizing ribosomes in proportion with uranium amendment up to 2 μM. Concentrations of U (VI) >2 μM were generally found to inhibit ribosome synthesis. Two activemore » bacteria responding to uranium addition in the field were close relatives of Desulfobacter postgateii and Geobacter bemidjiensis. Since RNA content often increases with growth rate, our findings suggest it is possible to rapidly elucidate active bacteria responding to the addition of uranium in field samples and provides a more targeted approach to stimulate specific populations to enhance radionuclide reduction in contaminated sites.« less

  4. Identification of bacteria synthesizing ribosomal RNA in response to uranium addition during biostimulation at the Rifle, CO Integrated Field Research site

    SciTech Connect (OSTI)

    McGuinness, Lora R.; Wilkins, Michael J.; Williams, Kenneth H.; Long, Philip E.; Kerkhof, Lee J.; Boyanov, Maxim I.

    2015-09-18

    Understanding which organisms are capable of reducing uranium at historically contaminated sites provides crucial information needed to evaluate treatment options and outcomes. One approach is determination of the bacteria which directly respond to uranium addition. In this research, uranium amendments were made to groundwater samples from a site of ongoing biostimulation with acetate. The active microbes in the planktonic phase were deduced by monitoring ribosomes production via RT-PCR. The results indicated several microorganisms were synthesizing ribosomes in proportion with uranium amendment up to 2 ?M. Concentrations of U (VI) >2 ?M were generally found to inhibit ribosome synthesis. Two active bacteria responding to uranium addition in the field were close relatives of Desulfobacter postgateii and Geobacter bemidjiensis. Since RNA content often increases with growth rate, our findings suggest it is possible to rapidly elucidate active bacteria responding to the addition of uranium in field samples and provides a more targeted approach to stimulate specific populations to enhance radionuclide reduction in contaminated sites.

  5. Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1998-05-26

    Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  6. Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1997-12-16

    Bacteria which produce large amounts of cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  7. Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig (Lenoir City, TN)

    1997-12-16

    Bacteria which produce large amounts of cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  8. Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig (Lenoir City, TN)

    1998-01-01

    Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  9. Bioprocessing of lignite coals using reductive microorganisms. Final technical report, September 30, 1988--March 29, 1992

    SciTech Connect (OSTI)

    Crawford, D.L.

    1992-03-29

    In order to convert lignite coals into liquid fuels, gases or chemical feedstock, the macromolecular structure of the coal must be broken down into low molecular weight fractions prior to further modification. Our research focused on this aspect of coal bioprocessing. We isolated, characterized and studied the lignite coal-depolymerizing organisms Streptomyces viridosporus T7A, Pseudomonas sp. DLC-62, unidentified bacterial strain DLC-BB2 and Gram-positive Bacillus megaterium strain DLC-21. In this research we showed that these bacteria are able to solubilize and depolymerize lignite coals using a combination of biological mechanisms including the excretion of coal solublizing basic chemical metabolites and extracellular coal depolymerizing enzymes.

  10. Method for sustaining microorganism culture in syngas fermentation process in decreased concentration or absence of various substrates

    DOE Patents [OSTI]

    Adams, Stephen S.; Scott, Syrona; Ko, Ching-Whan

    2015-05-19

    The present invention relates to methods for sustaining microorganism culture in a syngas fermentation reactor in decreased concentration or absence of various substrates comprising: adding carbon dioxide and optionally alcohol; maintaining free acetic acid concentrations; and performing the above mentioned steps within specified time.

  11. A C. elegans-based foam for rapid on-site detection of residual live virus.

    SciTech Connect (OSTI)

    Negrete, Oscar A.; Branda, Catherine; Hardesty, Jasper O. E.; Tucker, Mark David; Kaiser, Julia N.; Kozina, Carol L.; Chirica, Gabriela S.

    2012-02-01

    In the response to and recovery from a critical homeland security event involving deliberate or accidental release of biological agents, initial decontamination efforts are necessarily followed by tests for the presence of residual live virus or bacteria. Such 'clearance sampling' should be rapid and accurate, to inform decision makers as they take appropriate action to ensure the safety of the public and of operational personnel. However, the current protocol for clearance sampling is extremely time-intensive and costly, and requires significant amounts of laboratory space and capacity. Detection of residual live virus is particularly problematic and time-consuming, as it requires evaluation of replication potential within a eukaryotic host such as chicken embryos. The intention of this project was to develop a new method for clearance sampling, by leveraging Sandia's expertise in the biological and material sciences in order to create a C. elegans-based foam that could be applied directly to the entire contaminated area for quick and accurate detection of any and all residual live virus by means of a fluorescent signal. Such a novel technology for rapid, on-site detection of live virus would greatly interest the DHS, DoD, and EPA, and hold broad commercial potential, especially with regard to the transportation industry.

  12. Nuclear Physics Technology Saves Lives | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Physics Technology Saves Lives January 11, 2006 Listen to this story Ribbon With early ... Group, headed by Stan Majewski, is part of the Physics Division here at Jefferson Lab. ...

  13. Spatially-Resolved Analysis of Glycolipids and Metabolites in Living Synechococcus sp. PCC7002 Using Nanospray Desorption Electrospray Ionization

    SciTech Connect (OSTI)

    Lanekoff, Ingela T.; Geydebrekht, Oleg V.; Pinchuk, Grigoriy E.; Konopka, Allan; Laskin, Julia

    2013-04-07

    Microorganisms release a diversity of organic compounds that couple interspecies metabolism, enable communication, or provide benefits to other microbes. Increased knowledge of microbial metabolite production will contribute to understanding of the dynamic microbial world and can potentially lead to new developments in drug discovery, biofuel production, and clinical research. Nanospray desorption electrospray ionization (nano-DESI) is an ambient ionization technique that enables detailed chemical characterization of molecules from a specific location on a surface without special sample pretreatment. Due to its ambient nature, living bacterial colonies growing on agar plates can be rapidly and non-destructively analyzed. We performed spatially resolved nano-DESI analysis of living Synechococcus sp. PCC 7002 colonies on agar plates. We use high resolution mass spectrometry and MS/MS analysis of the living Synechococcus sp. PCC 7002 colonies to detect metabolites and lipids, and confirm their identities. We found that despite the high salt content of the agar (osmolarity ca. 700 mM), nano-DESI analysis enables detailed characterization of metabolites produced by the colony. Using this technique, we identified several glycolipids found on the living colonies and examined the effect of the age of the colony on the chemical gradient of glucosylglycerol secreted onto agar.

  14. Natural analogue studies of the role of colloids, natural organics and microorganisms on radionuclide transport

    SciTech Connect (OSTI)

    McCarthy, J.F.

    1994-10-01

    Colloids may be important as a geochemical transport mechanism for radionuclides at geological repositories if they are (1) present in the groundwater, (2) stable with respect to both colloidal and chemical stabilities, (3) capable of adsorbing radionuclides, especially if the sorption is irreversible, and (4) mobile in the subsurface. The available evidence from natural analogue and other field studies relevant to these issues is reviewed, as is the potential role of mobile microorganisms ({open_quotes}biocolloids{close_quotes}) on radionuclide migration. Studies have demonstrated that colloids are ubiquitous in groundwater, although colloid concentrations in deep, geochemically stable systems may be too low to affect radionuclide transport. However, even low colloid populations cannot be dismissed as a potential concern because colloids appear to be stable, and many radionuclides that adsorb to colloids are not readily desorbed over long periods. Field studies offer somewhat equivocal evidence concerning colloid mobility and cannot prove or disprove the significance of colloid transport in the far-field environment. Additional research is needed at new sites to properly represent a repository far-field. Performance assessment would benefit from natural analogue studies to examine colloid behavior at sites encompassing a suite of probable groundwater chemistries and that mimic the types of formations selected for radioactive waste repositories.

  15. Small Talk: Cell-to-Cell Communication in Bacteria

    ScienceCinema (OSTI)

    Bassler, Bonnie [Princeton University, Princeton, New Jersey, United States

    2010-01-08

    Cell-cell communication in bacteria involves the production, release, and subsequent detection of chemical signaling molecules called autoinducers. This process, called quorum sensing, allows bacteria to regulate gene expression on a population-wide scale. Processes controlled by quorum sensing are usually ones that are unproductive when undertaken by an individual bacterium but become effective when undertaken by the group. For example, quorum sensing controls bioluminescence, secretion of virulence factors, biofilm formation, sporulation, and the exchange of DNA. Thus, quorum sensing is a mechanism that allows bacteria to function as multi-cellular organisms. Bacteria make, detect, and integrate information from multiple autoinducers, some of which are used exclusively for intra-species communication while others enable communication between species. Research is now focused on the development of therapies that interfere with quorum sensing to control bacterial virulence.

  16. Multiple Species of Bacteria Convert Elemental Mercury to Toxic...

    Office of Science (SC) Website

    Researchers are studying how bacteria transform mercury into a toxic form in the environment that can accumulate in the food web, posing a threat to wildlife and people. The ...

  17. Hydrogen (H2) Production by Anoxygenic Purple Nonsulfur Bacteria |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Anoxygenic Purple Nonsulfur Bacteria Hydrogen (H2) Production by Anoxygenic Purple Nonsulfur Bacteria Presentation by Jake McKinlay, Indiana University, at the Biological Hydrogen Production Workshop held September 24-25, 2013, at the National Renewable Energy Laboratory in Golden, Colorado. PDF icon bio_h2_workshop_mckinlay.pdf More Documents & Publications 2013 Biological Hydrogen Production Workshop Summary Report Savannah River National Laboratory (SRNL)

  18. Material and method for promoting the growth of anaerobic bacteria

    DOE Patents [OSTI]

    Adler, Howard I. (128 Indian La., Oak Ridge, TN 37830)

    1984-01-01

    A material and method for promoting the growth of anaerobic bacteria which includes a nutrient media containing a hydrogen donor and sterile membrane fragments of bacteria having an electron transfer system which reduces oxygen to water. Dissolved oxygen in the medium is removed by adding the sterile membrane fragments to the nutrient medium and holding the medium at a temperature of about 10.degree. to about 60.degree. C. until the dissolved oxygen is removed.

  19. Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced

    Office of Scientific and Technical Information (OSTI)

    Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). (Conference) | SciTech Connect Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). Citation Details In-Document Search Title: Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). Abstract not provided. Authors: Reichmuth, David ; Kozina, Carol L. ; Sale, Kenneth L. ;

  20. Material and method for promoting the growth of anaerobic bacteria

    DOE Patents [OSTI]

    Adler, H.I.

    1984-10-09

    A material and method is disclosed for promoting the growth of anaerobic bacteria which includes a nutrient media containing a hydrogen donor and sterile membrane fragments of bacteria having an electron transfer system which reduces oxygen to water. Dissolved oxygen in the medium is removed by adding the sterile membrane fragments to the nutrient medium and holding the medium at a temperature of about 10 to about 60 C until the dissolved oxygen is removed. No Drawings

  1. Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of

    Office of Scientific and Technical Information (OSTI)

    Ethanol from 5-Carbon Sugars (LDRD %23 105944). (Conference) | SciTech Connect Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). Citation Details In-Document Search Title: Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). Abstract not provided. Authors: Sapra, Rajat ; Reichmuth, David ; Kozina, Carol L. ; Sale, Kenneth L. ; Keasling, Jay ; Tang,

  2. Influence of microorganisms on the oxidation state distribution of multivalent actinides under anoxic conditions

    SciTech Connect (OSTI)

    Reed, Donald Timothy; Borkowski, Marian; Lucchini, Jean - Francois; Ams, David; Richmann, M. K.; Khaing, H.; Swanson, J. S.

    2010-12-10

    The fate and potential mobility of multivalent actinides in the subsurface is receiving increased attention as the DOE looks to cleanup the many legacy nuclear waste sites and associated subsurface contamination. Plutonium, uranium and neptunium are the near-surface multivalent contaminants of concern and are also key contaminants for the deep geologic disposal of nuclear waste. Their mobility is highly dependent on their redox distribution at their contamination source as well as along their potential migration pathways. This redox distribution is often controlled, especially in the near-surface where organic/inorganic contaminants often coexist, by the direct and indirect effects of microbial activity. Under anoxic conditions, indirect and direct bioreduction mechanisms exist that promote the prevalence of lower-valent species for multivalent actinides. Oxidation-state-specific biosorption is also an important consideration for long-term migration and can influence oxidation state distribution. Results of ongoing studies to explore and establish the oxidation-state specific interactions of soil bacteria (metal reducers and sulfate reducers) as well as halo-tolerant bacteria and Archaea for uranium, neptunium and plutonium will be presented. Enzymatic reduction is a key process in the bioreduction of plutonium and uranium, but co-enzymatic processes predominate in neptunium systems. Strong sorptive interactions can occur for most actinide oxidation states but are likely a factor in the stabilization of lower-valent species when more than one oxidation state can persist under anaerobic microbiologically-active conditions. These results for microbiologically active systems are interpreted in the context of their overall importance in defining the potential migration of multivalent actinides in the subsurface.

  3. HMPT: Hazardous Waste Transportation Live 27928, Test 27929 ...

    Office of Scientific and Technical Information (OSTI)

    HMPT: Hazardous Waste Transportation Live 27928, Test 27929 Citation Details In-Document Search Title: HMPT: Hazardous Waste Transportation Live 27928, Test 27929 HMPT: Hazardous ...

  4. Senior Adviser Richard Kauffman to Host Live Chat on Renewable...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and Deployment Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and...

  5. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Tracking Living Cells as They Differentiate in Real Time Print Thursday, 27 September 2012 00:00 Protein phosphorylation ...

  6. Senior Adviser Richard Kauffman to Host Live Chat on Renewable...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and Deployment Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and ...

  7. JV between KP Renewables and Living Buildings | Open Energy Informatio...

    Open Energy Info (EERE)

    KP Renewables and Living Buildings Jump to: navigation, search Name: JV between KP Renewables and Living Buildings Place: Brentford, Middlesex, Greater London, United Kingdom Zip:...

  8. An Approach for Assessing the Signature Quality of Various Chemical Assays when Predicting the Culture Media Used to Grow Microorganisms

    SciTech Connect (OSTI)

    Holmes, Aimee E.; Sego, Landon H.; Webb-Robertson, Bobbie-Jo M.; Kreuzer, Helen W.; Anderson, Richard M.; Unwin, Stephen D.; Weimar, Mark R.; Tardiff, Mark F.; Corley, Courtney D.

    2013-02-01

    We demonstrate an approach for assessing the quality of a signature system designed to predict the culture medium used to grow a microorganism. The system was comprised of four chemical assays designed to identify various ingredients that could be used to produce the culture medium. The analytical measurements resulting from any combination of these four assays can be used in a Bayesian network to predict the probabilities that the microorganism was grown using one of eleven culture media. We evaluated combinations of the signature system by removing one or more of the assays from the Bayes network. We measured and compared the quality of the various Bayes nets in terms of fidelity, cost, risk, and utility, a method we refer to as Signature Quality Metrics

  9. Early Detection Saves Lives | Department of Energy

    Office of Environmental Management (EM)

    Early Detection Saves Lives Early Detection Saves Lives September 21, 2012 - 9:49am Addthis What does this mean for me? It can save your life. It can save the life of someone you love. The Worker Health Protection Program (WHPP) is the DOE's Former Worker Medical Screening Program at 13 DOE sites. WHPP provides free medical evaluations for selected occupational diseases every three years to eligible former DOE workers under a national medical protocol established by the DOE. WHPP is funded by

  10. Live Webinar on Better Buildings Challenge: Public-Sector Update

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webinar titled "Better Buildings Challenge: Public-Sector Update."

  11. Portsmouth Site Feeds Bacteria to Render Hazardous Groundwater Waste

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Harmless | Department of Energy Feeds Bacteria to Render Hazardous Groundwater Waste Harmless Portsmouth Site Feeds Bacteria to Render Hazardous Groundwater Waste Harmless April 2, 2012 - 12:00pm Addthis Neil Smith puts a trained eye on the pressure and flow of a food-grade com¬pound being injected into an under¬ground plume of hazardous waste near the X-720 Maintenance Facility at the DOE Piketon Site. The sodium lactate compound promotes bacterial growth in the groundwater that turns

  12. Utilizing Bacteria for Sustainable Manufacturing of Low-Cost Nanoparticles

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bacteria for Sustainable Manufacturing of Low-Cost Nanoparticles Chad Duty, Ph.D. Technical Lead Additive Manufacturing Roll-to-Roll Processing June 26, 2012 2 Managed by UT-Battelle for the U.S. Department of Energy Presentation_name NanoFermentation 3 Managed by UT-Battelle for the U.S. Department of Energy Presentation_name NanoFermentation Magnetic Semiconducting Photovoltaic Phosphor Cheap oxidized bulk salts Natural bacteria Valuable mass produced nanoparticles Cheap sugar 4 Managed by

  13. Fission barriers and half-lives

    SciTech Connect (OSTI)

    Moeller, P.; Nix, J.R.; Swiatecki, W.J.

    1989-01-01

    We briefly review the development of theoretical models for the calculation of fission barriers and half-lives. We focus on how results of actual calculations in a unified macroscopic-microscopic approach provide an interpretation of the mechanisms behind some of the large number of phenomena observed in fission. As instructive examples we choose studies of the rapidly varying fission properties of elements at the end of the periodic system. 31 refs., 10 figs.

  14. Method for establishing the presence of salmonella bacteria in eggs

    DOE Patents [OSTI]

    Johnston, Roger G. (Los Alamos, NM); Sinha, Dipen N. (Los Alamos, NM)

    1995-01-01

    Measurement of the acoustical resonances in eggs is shown to provide a rapid, noninvasive technique for establishing the presence of Salmonella bacteria. The technique is also sensitive to yolk puncture, shell cracks, and may be sensitive to other yolk properties and to egg freshness. Remote characterization, potentially useful for characterizing large numbers of eggs, has been demonstrated.

  15. Methods for targetted mutagenesis in gram-positive bacteria

    DOE Patents [OSTI]

    Yang, Yunfeng

    2014-05-27

    The present invention provides a method of targeted mutagenesis in Gram-positive bacteria. In particular, the present invention provides a method that effectively integrates a suicide integrative vector into a target gene in the chromosome of a Gram-positive bacterium, resulting in inactivation of the target gene.

  16. New Advances in Optical Imaging of Live Cells and Organisms

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New Advances in Optical Imaging of Live Cells and Organisms New Advances in Optical Imaging of Live Cells and Organisms Print http://csh-asia.com/13image.html August 20-23, 2913; Suzhou, China

  17. New Advances in Optical Imaging of Live Cells and Organisms

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in Optical Imaging of Live Cells and Organisms New Advances in Optical Imaging of Live Cells and Organisms Print http:csh-asia.com13image.html August 20-23, 2913; Suzhou, China...

  18. Hanwha L C Hanwha Living and Creative | Open Energy Information

    Open Energy Info (EERE)

    Hanwha L C Hanwha Living and Creative Jump to: navigation, search Name: Hanwha L&C (Hanwha Living and Creative) Place: Seoul, Seoul, Korea (Republic) Zip: 100-797 Sector: Solar...

  19. DOE Tour of Zero Floorplans: Options for Community Living by...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Options for Community Living by United Way of Long Island DOE Tour of Zero Floorplans: Options for Community Living by United Way of Long Island DOE Tour of Zero Floorplans:...

  20. Green Living, Green Technologies: Things to Be Thankful For

    Broader source: Energy.gov [DOE]

    We live in a time where improvements in technology make it possible to live a happy, healthy, and productive life while using less and less energy. Isn't that something for which we should give thanks?

  1. Safety assessment of outdoor live fire range

    SciTech Connect (OSTI)

    1989-05-01

    The following Safety Assessment (SA) pertains to the outdoor live fire range facility (LFR). The purpose of this facility is to supplement the indoor LFR. In particular it provides capacity for exercises that would be inappropriate on the indoor range. This SA examines the risks that are attendant to the training on the outdoor LFR. The outdoor LFR used by EG&G Mound is privately owned. It is identified as the Miami Valley Shooting Grounds. Mondays are leased for the exclusive use of EG&G Mound.

  2. The living publication (Technical Report) | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    The living publication Citation Details In-Document Search Title: The living publication Within the ICSTI Insights Series we offer three articles on the 'living publication' that is already available to practitioners in the important field of crystal structure determination and analysis. While the specific examples are drawn from this particular field, we invite readers to draw parallels in their own fields of interest. The first article describes the present state of the crystallographic living

  3. Live Broadcast on Cyber Distinguished Speaker Series | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Live Broadcast on Cyber Distinguished Speaker Series Live Broadcast on Cyber Distinguished Speaker Series Live streaming video by Ustream Evan D. Wolff, a partner with Crowell & Moring, will discuss data breaches, risk management, and incident response plans as part of the DOE Cyber Distinguished Series. DETAILS: February 16, 2016 at 1:00pm EST Learn More About Evan Wolff DOE Cyber Strategy

  4. Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio

    SciTech Connect (OSTI)

    Chhabra, Swapnil R; Keller, Kimberly L.; Wall, Judy D.

    2011-03-15

    Sulfate reducing bacteria are physiologically important given their nearly ubiquitous presence and have important applications in the areas of bioremediation and bioenergy. This chapter provides details on the steps used for homologous-recombination mediated chromosomal manipulation of Desulfovibrio vulgaris Hildenborough, a well-studied sulfate reducer. More specifically, we focus on the implementation of a 'parts' based approach for suicide vector assembly, important aspects of anaerobic culturing, choices for antibiotic selection, electroporation-based DNA transformation, as well as tools for screening and verifying genetically modified constructs. These methods, which in principle may be extended to other sulfate-reducing bacteria, are applicable for functional genomics investigations, as well as metabolic engineering manipulations.

  5. Universal Gene Transfer Technology for Gram Positive Bacteria - Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Innovation Portal Biomass and Biofuels Biomass and Biofuels Advanced Materials Advanced Materials Find More Like This Return to Search Universal Gene Transfer Technology for Gram Positive Bacteria Oak Ridge National Laboratory Contact ORNL About This Technology Publications: PDF Document Publication 11-G00255_ID2139_rev.pdf (493 KB) Technology Marketing SummaryA genetic engineering technology invented at ORNL facilitates DNA delivery to a cell by using ultrasound to permeate the cell's

  6. Enhanced Biomass Digestion with Wood Wasp Bacteria - Energy Innovation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Portal Enhanced Biomass Digestion with Wood Wasp Bacteria Great Lakes Bioenergy Research Center Contact GLBRC About This Technology Technology Marketing Summary Plant biomass represents a vast and renewable source of energy. However, harnessing this energy requires breaking down tough lignin and cellulose cell walls. In nature, certain microbes can deconstruct biomass into simple sugars by secreting combinations of enzymes. Two organisms that utilize cellulose are Clostridium thermocellum -

  7. Secret Lives of Scientists: Matt Kwiatkowski | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Secret Lives of Scientists: Matt Kwiatkowski Secret Lives of Scientists & Engineers: Matt Kwiatkowski 1 of 16 Secret Lives of Scientists & Engineers: Matt Kwiatkowski Matt Kwiatkowski is a cyber security manager at Argonne National Lab - and flies over it in a plane he built himself during the weekends. Read the full story » Photos by Mark Lopez/Argonne National Laboratory. 31296D Secret Lives of Scientists & Engineers: Matt Kwiatkowski 1 of 16 Secret Lives of

  8. Elimination of mercury and organomercurials by nitrogen-fixing bacteria

    SciTech Connect (OSTI)

    Ghosh, S.; Sadhukhan, P.C.; Ghosh, D.K.

    1997-06-01

    Bacteria isolated from mercury-polluted environments are often resistant to mercuric ions (Hg{sup 2+}) and organomercurials. Plasmids determining mercury resistance have been well characterized in gram-negative system. However, in Staphylococcus aureus mercury resistance has been found to be chromosomally determined. The known mechanism of bacterial Hg{sup 2+}-resistance is detoxification of the toxic Hg{sup 2+} by its enzymatic transformation by mercuric reductase to Hg (o). Organomercurial lyase mediates the degradation of organomercurial compounds to Hg{sup 2+}. Mercury and organomercurial resistances have been studied in different bacterial genera. There is little information on Hg-resistance in N{sub 2}-fixing soil bacteria, however, in many developing countries, including India, mercury pollution is still a problem because Hg-based pesticides and fungicides are still used routinely as seed-dressers in agriculture to control soil-borne and seed-borne fungal diseases. Volatilization of Hg from laboratory media by mercury-resistant bacteria containing low levels of mercury has been reported by several workers. It is interesting to note that N{sub 2}-fixing, Hg-resistant soil isolates could volatilize Hg from medium containing very high amounts of HgCl{sub 2}. In the present paper we report the volatilization patterns of five N{sub 2}-fixing bacterial strains, the effect of different inducers on mercuric reductase, and the pattern of substrate utilization by organomercurial lyase. In the presence of a low concentration of HgCl{sub 2}. enzymatic detoxification is sufficient to combat the adverse situation created by the presence of Hg{sup 2+} ions. In the presence of a high concentration of HgCl{sub 2}, intracellular sequestration by Hg{sup 2+} binding components may play an additional role in counteracting Hg-toxicity.

  9. Engineering Bacteria for Efficient Fuel Production: Novel Biological Conversion of Hydrogen and Carbon Dioxide Directly into Free Fatty Acids

    SciTech Connect (OSTI)

    2010-07-12

    Electrofuels Project: OPX Biotechnologies is engineering a microorganism currently used in industrial biotechnology to directly produce a liquid fuel from hydrogen and carbon dioxide (CO2). The microorganism has the natural ability to use hydrogen and CO2 for growth. OPX Biotechnologies is modifying the microorganism to divert energy and carbon away from growth and towards the production of liquid fuels in larger, commercially viable quantities. The microbial system will produce a fuel precursor that can be chemically upgraded to various hydrocarbon fuels.

  10. Detergent composition comprising a cellulase containing cell-free fermentate produced from microorganism ATCC 55702 or mutant thereof

    DOE Patents [OSTI]

    Dees, H. Craig (Lenoir City, TN)

    1998-01-01

    Bacteria which produce large amounts of a cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  11. Detergent composition comprising a cellulase containing cell-free fermentate produced from microorganism ATCC 55702 or mutant thereof

    DOE Patents [OSTI]

    Dees, H.C.

    1998-07-14

    Bacteria which produce large amounts of a cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  12. Picture of the Week: Explosive science to save lives

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    6 Explosive science to save lives During Homemade Explosives (HME) training courses at Los Alamos National Laboratory, technicians from the Navy, Marines, and Air Force focus on world-wide HME threats including the safety, sensitivity and performance of these explosives, and their synthesis and manufacturing. September 13, 2015 x x Explosive science to save lives Realistic explosive science and detection training gives military personnel the tools to save lives when they encounter explosive

  13. An ultra-sensitive microfluidic immunoassay using living radical

    Office of Scientific and Technical Information (OSTI)

    polymerization and porous polymer monoliths. (Conference) | SciTech Connect Conference: An ultra-sensitive microfluidic immunoassay using living radical polymerization and porous polymer monoliths. Citation Details In-Document Search Title: An ultra-sensitive microfluidic immunoassay using living radical polymerization and porous polymer monoliths. We present a platform that combines patterned photopolymerized polymer monoliths with living radical polymerization (LRP) to develop a low cost

  14. Live webcast on groundbreaking results of Algal Biofuels Consortium, June

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    11 Live webcast on Algal Biofuels Consortium Live webcast on groundbreaking results of Algal Biofuels Consortium, June 11 Jose A. Olivares will present an overview of the technologies and processes that are needed to widely produce algae-based liquid transportation fuels. June 10, 2014 Jose A. Olivares Jose A. Olivares Contact Nancy Ambrosiano Communications Office (505) 667-0471 Email The Energy Department will present a live webcast titled "Algal Biofuels Consortium Releases

  15. National Clean Energy Business Plan Competition: Living Ink Technologies

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Wins CU Clean Energy Competition Regional Championship | Department of Energy Living Ink Technologies Wins CU Clean Energy Competition Regional Championship National Clean Energy Business Plan Competition: Living Ink Technologies Wins CU Clean Energy Competition Regional Championship May 4, 2015 - 2:51pm Addthis Living Ink Technologies has developed a patent-pending technology that uses algae to transform carbon dioxide into ink that is cheaper, healthier, and more environmentally

  16. Electromicroinjection of particles into living cells

    DOE Patents [OSTI]

    Ray, F. Andrew (Los Alamos, NM); Cram, L. Scott (Los Alamos, NM); Galey, William R. (Albuquerque, NM)

    1988-01-01

    Method and apparatus for introducing particles into living cells. Fluorescently-stained human chromosomes are introduced into cultured, mitotic Chinese hamster cells using electromicroinjection. The recipient cells frequently survived the physiological perturbation imposed by a successful chromosome injection. Successfully injected recipient cells maintained viability as evidenced by their ability to be expanded. The technique relies on the surface charge of fluorescently stained chromosomes and their ability to be attracted and repelled to and from the tip of a micropipette. The apparatus includes a micropipette having a tip suitable for piercing the membrane of a target cell and an electrode inserted into the lumen thereof. The target cells and suspended particles are located in an electrically conducted solution, and the lumen of the micropipette is filled with an electrically conducting solution which contacts the electrode located therein. A second electrode is also located in the conducting solution containing the target cells and particles. Voltages applied to the electrode within the micropipette attract the particles to the region of the tip thereof. The particles adhere to the surface of the micropipette with sufficient force that insertion of the micropipette tip and attached particle through the membrane of a target cell will not dislodge the particle. By applying a voltage having the opposite polarity of the attraction voltage, the particles are expelled from the micropipette to which is then withdrawn from the cell body.

  17. New Advances in Optical Imaging of Live Cells and Organisms

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New Advances in Optical Imaging of Live Cells and Organisms http://csh-asia.com/13image.html August 20-23, 2913; Suzhou, China

  18. University Teams to Showcase Affordable, Energy Efficient Living...

    Office of Environmental Management (EM)

    Efficient Living in U.S. Department of Energy Solar Decathlon 2011 University Teams to ... appeal, and design excellence with optimal energy production and maximum efficiency. ...

  19. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation ... cells, enabling them to follow cellular chemical changes in real time, without bias. ...

  20. Solar Decathlon Team Leading the Way Toward Sustainable Living...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Team Leading the Way Toward Sustainable Living, Even in the Wake of Disasters Solar ... | Courtesy of the University of Illinois at Urbana-Champaign Solar Decathlon Team. ...

  1. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins...

  2. Dutch Company Powers Streetlights With Living Plants; Will Your...

    Open Energy Info (EERE)

    Dutch Company Powers Streetlights With Living Plants; Will Your Cell Phone Be Next? Home > Groups > OpenEI Community Central Dc's picture Submitted by Dc(266) Contributor 16...

  3. Spontaneous fission half-lives for ground state nuclides

    SciTech Connect (OSTI)

    Holden, N.E. [Brookhaven National Lab., Upton, NY (United States); Hoffman, D.C. [Lawrence Berkeley Lab., CA (United States)

    1993-08-01

    Measurements of the spontaneous fission half-lives of nuclides of elements Z = 90 to 108 have been compiled and evaluated. Recommended values are presented.

  4. Watch a Rare Earth Elements Event Live This Morning

    Broader source: Energy.gov [DOE]

    From 9:30am to noon ET today you can tune into a live discussion rare earth materialsmthat are critical to the production of clean energy technologies.

  5. Brownsville Public Utilities Board- Green Living Residential Rebate Program

    Broader source: Energy.gov [DOE]

     Brownsville Public Utilities Board offers residential customers rebates for installation of energy efficient measures. Through the Green Living Rebate program, customers can apply for rebates for...

  6. Pathway of Fermentative Hydrogen Production by Sulfate-reducing Bacteria

    SciTech Connect (OSTI)

    Wall, Judy D.

    2015-02-16

    Biofuels are a promising source of sustainable energy. Such biofuels are intermediate products of microbial metabolism of renewable substrates, in particular, plant biomass. Not only are alcohols and solvents produced in this degradative process but energy-rich hydrogen as well. Non photosynthetic microbial hydrogen generation from compounds other than sugars has not been fully explored. We propose to examine the capacity of the abundant soil anaerobes, sulfate-reducing bacteria, for hydrogen generation from organic acids. These apparently simple pathways have yet to be clearly established. Information obtained may facilitate the exploitation of other microbes not yet readily examined by molecular tools. Identification of the flexibility of the metabolic processes to channel reductant to hydrogen will be useful in consideration of practical applications. Because the tools for genetic and molecular manipulation of sulfate-reducing bacteria of the genus Desulfovibrio are developed, our efforts will focus on two strains, D. vulgaris Hildenborough and Desulfovibrio G20.Therefore total metabolism, flux through the pathways, and regulation are likely to be limiting factors which we can elucidate in the following experiments.

  7. Evidence of radicals created by plasma in bacteria in water

    SciTech Connect (OSTI)

    Lee, Chae Bok; Na, Young Ho; Hong, Tae-Eun; Choi, Eun Ha; Uhm, Han S.; Baik, Ku Youn E-mail: gckwon@kw.ac.kr; Kwon, Gichung E-mail: gckwon@kw.ac.kr

    2014-08-18

    Heavy water (D{sub 2}O) was introduced into a non-thermal plasma-jet (NTPJ) device to generate deuterium monoxide (OD) radicals at room temperature. Owing to the similar reactivity and low prevalence of deuterium in nature, OD radicals can be utilized to visualize the OH radical interactions with water and living cells. Escherichia coli in water were treated with OD radicals, and D atom incorporation into cells was visualized using time-of-flight SIMS and Nano-SIMS. The results show that D atoms from NTPJ reach the cytoplasm of E. coli in H{sub 2}O, indicating the usefulness of this OD-tracking method for the study of radical interactions with living cells.

  8. Virtual Institute of Microbial Stress and Survival: Deduction of Stress Response Pathways in Metal and Radionuclide Reducing Microorganisms

    SciTech Connect (OSTI)

    2004-04-17

    The projects application goals are to: (1) To understand bacterial stress-response to the unique stressors in metal/radionuclide contamination sites; (2) To turn this understanding into a quantitative, data-driven model for exploring policies for natural and biostimulatory bioremediation; (3) To implement proposed policies in the field and compare results to model predictions; and (4) Close the experimental/computation cycle by using discrepancies between models and predictions to drive new measurements and construction of new models. The projects science goals are to: (1) Compare physiological and molecular response of three target microorganisms to environmental perturbation; (2) Deduce the underlying regulatory pathways that control these responses through analysis of phenotype, functional genomic, and molecular interaction data; (3) Use differences in the cellular responses among the target organisms to understand niche specific adaptations of the stress and metal reduction pathways; (4) From this analysis derive an understanding of the mechanisms of pathway evolution in the environment; and (5) Ultimately, derive dynamical models for the control of these pathways to predict how natural stimulation can optimize growth and metal reduction efficiency at field sites.

  9. Process for generation of hydrogen gas from various feedstocks using thermophilic bacteria

    DOE Patents [OSTI]

    Ooteghem, Suellen Van (Morgantown, WV)

    2005-09-13

    A method for producing hydrogen gas is provided comprising selecting a bacteria from the Order Thermotogales, subjecting the bacteria to a feedstock and to a suitable growth environment having an oxygen concentration below the oxygen concentration of water in equilibrium with air; and maintaining the environment at a predetermined pH and at a temperature of at least approximately 45.degree. C. for a time sufficient to allow the bacteria to metabolize the feedstock.

  10. Harnessing the Sun: Energy in Our Lives | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Harnessing the Sun: Energy in Our Lives Harnessing the Sun: Energy in Our Lives Addthis Innovation Workshop: Harnessing the Sun highlights the science and engineering of how radiant energy is transformed into other useful forms of energy. Watch the rest of the videos

  11. Rocky Flats Plant Live-Fire Range Risk Analysis Report

    SciTech Connect (OSTI)

    Nicolosi, S.L.; Rodriguez, M.A.

    1994-04-01

    The objective of the Live-Fire Range Risk Analysis Report (RAR) is to provide an authorization basis for operation as required by DOE 5480.16. The existing Live-Fire Range does not have a safety analysis-related authorization basis. EG&G Rocky Flats, Inc. has worked with DOE and its representatives to develop a format and content description for development of an RAR for the Live-Fire Range. Development of the RAR is closely aligned with development of the design for a baffle system to control risks from errant projectiles. DOE 5480.16 requires either an RAR or a safety analysis report (SAR) for live-fire ranges. An RAR rather than a SAR was selected in order to gain flexibility to more closely address the safety analysis and conduct of operation needs for a live-fire range in a cost-effective manner.

  12. Selection against spurious promoter motifs correlates withtranslational efficiency across bacteria

    SciTech Connect (OSTI)

    Froula, Jeffrey L.; Francino, M. Pilar

    2007-05-01

    Because binding of RNAP to misplaced sites could compromise the efficiency of transcription, natural selection for the optimization of gene expression should regulate the distribution of DNA motifs capable of RNAP-binding across the genome. Here we analyze the distribution of the -10 promoter motifs that bind the {sigma}{sup 70} subunit of RNAP in 42 bacterial genomes. We show that selection on these motifs operates across the genome, maintaining an over-representation of -10 motifs in regulatory sequences while eliminating them from the nonfunctional and, in most cases, from the protein coding regions. In some genomes, however, -10 sites are over-represented in the coding sequences; these sites could induce pauses effecting regulatory roles throughout the length of a transcriptional unit. For nonfunctional sequences, the extent of motif under-representation varies across genomes in a manner that broadly correlates with the number of tRNA genes, a good indicator of translational speed and growth rate. This suggests that minimizing the time invested in gene transcription is an important selective pressure against spurious binding. However, selection against spurious binding is detectable in the reduced genomes of host-restricted bacteria that grow at slow rates, indicating that components of efficiency other than speed may also be important. Minimizing the number of RNAP molecules per cell required for transcription, and the corresponding energetic expense, may be most relevant in slow growers. These results indicate that genome-level properties affecting the efficiency of transcription and translation can respond in an integrated manner to optimize gene expression. The detection of selection against promoter motifs in nonfunctional regions also implies that no sequence may evolve free of selective constraints, at least in the relatively small and unstructured genomes of bacteria.

  13. Establishment of stable synthetic mutualism without co-evolution between microalgae and bacteria demonstrated by mutual transfer of metabolites (NanoSIMS isotopic imaging) and persistent physical association (Fluorescent in situ hybridization)

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    de-Bashan, Luz E.; Mayali, Xavier; Bebout, Brad M.; Weber, Peter K.; Detweiler, Angela M.; Hernandez, Juan- Pablo; Prufert-Bebout, Leslie; Bashan, Yoav

    2016-03-03

    The demonstration of a mutualistic interaction requires evidence of benefits for both partners as well as stability of the association over multiple generations. A synthetic mutualism between the freshwater microalga Chlorella sorokiniana and the soil-derived plant growth-promoting bacterium (PGPB) Azospirillum brasilense was created when both microorganisms were co-immobilized in alginate beads. Using stable isotope enrichment experiments followed by high-resolution secondary ion mass spectrometry (SIMS) imaging of single cells, we demonstrated transfer of carbon and nitrogen compounds between the two partners. Further, using fluorescent in situ hybridization (FISH), mechanical disruption and scanning electron microscopy, we demonstrated the stability of their physicalmore » association for a period of 10 days after the aggregated cells were released from the beads. The bacteria significantly enhanced the growth of the microalgae while the microalgae supported growth of the bacteria in a medium where it could not otherwise grow. In conclusion, we propose that this microalga-bacterium association is a true synthetic mutualism independent of co-evolution. (155 words).« less

  14. Radiation-resistant microorganism

    DOE Patents [OSTI]

    Fliermans, Carl B.

    2010-06-15

    An isolated and purified bacterium is provided which was isolated from a high-level radioactive waste site of mixed waste. The isolate has the ability to degrade a wide variety of organic contaminants while demonstrating high tolerance to ionizing radiation. The organism is uniquely suited to bioremediation of a variety or organic contaminants while in the presence of ionizing radiation.

  15. Butanol tolerance in microorganisms

    DOE Patents [OSTI]

    Bramucci, Michael G.; Nagarajan, Vasantha

    2016-03-01

    Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products from a pyruvate utilizing pathway. Yeast host cells provided herein comprise reduced pyruvate decarboxylase activity and modified adenylate cyclase activity. In embodiments, yeast host cells provided herein comprise resistance to butanol and increased biomass production.

  16. Radiation-resistant microorganism

    DOE Patents [OSTI]

    Fliermans, Carl B.

    2007-01-09

    An isolated and purified bacterium is provided which was isolated from a high-level radioactive waste site of mixed waste. The isolate has the ability to degrade a wide variety of organic contaminants while demonstrating high tolerance to ionizing radiation. The organism is uniquely suited to bioremediation of a variety or organic contaminants while in the presence of ionizing radiation.

  17. Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

    DOE Patents [OSTI]

    Bavykin, Sergei G. (Darien, IL); Mirzabekova, legal representative, Natalia V. (Westmont, IL); Mirzabekov, deceased, Andrei D. (Westmont, IL)

    2007-12-04

    The present invention relates to methods and compositions for using nucleotide sequence variations of 16S and 23S rRNA within the B. cereus group to discriminate a highly infectious bacterium B. anthracis from closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations and discriminate B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed samples, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

  18. Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

    DOE Patents [OSTI]

    Bavykin, Sergei G. (Darien, IL); Mirzabekov, Andrei D. (Moscow, RU)

    2007-10-30

    The present invention is directed to a novel method of discriminating a highly infectious bacterium Bacillus anthracis from a group of closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations. The identification and analysis of these sequence variations enables positive discrimination of isolates of the B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed probes, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

  19. Final technical report for project titled Quantitative Characterization of Cell Aggregation/Adhesion as Predictor for Distribution and Transport of Microorganisms in Subsurface Environment

    SciTech Connect (OSTI)

    Gu, April Z; Wan, Kai-tak

    2014-09-02

    This project aims to explore and develop enabling methodology and techniques for nano-scale characterization of microbe cell surface contact mechanics, interactions and adhesion quantities that allow for identification and quantification of indicative properties related to microorganism migration and transport behavior in porous media and in subsurface environments. Microbe transport has wide impact and therefore is of great interest in various environmental applications such as in situ or enhanced subsurface bioremediation,filtration processes for water and wastewater treatments and protection of drinking water supplies. Although great progress has been made towards understanding the identities and activities of these microorganisms in the subsurface, to date, little is known of the mechanisms that govern the mobility and transport of microorganisms in DOEs contaminated sites, making the outcomes of in situ natural attenuation or contaminant stability enhancement unpredictable. Conventionally, movement of microorganisms was believed to follows the rules governing solute (particle) transport. However, recent studies revealed that cell surface properties, especially those pertaining to cell attachment/adhesion and aggregation behavior, can cause the microbe behavior to deviate from non-viable particles and hence greatly influence the mobility and distribution of microorganisms in porous media.This complexity highlights the need to obtain detailed information of cell-cell and cell-surface interactions in order to improve and refine the conceptual and quantitative model development for fate and transport of microorganisms and contaminant in subsurface. Traditional cell surface characterization methods are not sufficient to fully predict the deposition rates and transport behaviors of microorganism observed. A breakthrough of methodology that would allow for quantitative and molecular-level description of intrinsic cell surface properties indicative for cell-surface interactions is essential for the field. To tackle this, we have developed a number of new Bio-nanomechanical techniques, including reflection interference contrast microscopy (RICM) and bio-AFM (Atomic Force Microscopy), for cell adhesion-detachment measurement of the long-range surface interactions, in combination with mathematical modeling, which would allow us to characterize the mechanical behavior from single cell to multi-cell aggregate, critical thresholds for large scale coaggregation and transportation of cells and aggregates in the presence of long range inter-surface forces etc. Although some technical and mathematical challenges remain, the preliminary results promise great breakthrough potential. In this study, we investigated the cellular surface characteristics of representative bio-remediating microorganisms relevant to DOE IFRC (Integrated Field-Scale Subsurface Research Challenges) sites and their transport behaviors in porous media, aiming to draw a groundbreaking correlation between the micro-scale genetic and biological origin-based cell surface properties, the consequent mechanical adhesion and aggregation behaviors, and the macro-scale microbial mobility and retention in porous media, which are unavailable in the literature. The long-term goal is to significantly improve the mechanistic and quantitative understanding of microbial mobility, sorption, and transport within reactive transport models as needed to manipulate subsurface contaminant fate and transport predictions.

  20. Tuesday Talk: Secretary Chu Answers Your Questions LIVE | Department of

    Energy Savers [EERE]

    Energy Tuesday Talk: Secretary Chu Answers Your Questions LIVE Tuesday Talk: Secretary Chu Answers Your Questions LIVE November 30, 2010 - 12:02pm Addthis John Schueler John Schueler Former New Media Specialist, Office of Public Affairs Later today Secretary Chu will be answering your questions as a part of the White House's Tuesday Talk series. The discussion will be broadcast live starting at 1:15 EST and will build off of the Secretary's speech at the National Press Club yesterday, which

  1. Clay enhancement of methane, low molecular weight hydrocarbon and halocarbon conversion by methanotrophic bacteria

    DOE Patents [OSTI]

    Apel, William A. (Idaho Falls, ID); Dugan, Patrick R. (Idaho Falls, ID)

    1995-01-01

    An apparatus and method for increasing the rate of oxidation of toxic vapors by methanotrophic bacteria. The toxic vapors of interest are methane and trichloroethylene. The apparatus includes a gas phase bioreactor within a closed loop pumping system or a single pass system. The methanotrophic bacteria include Methylomonas methanica, Methylosinus trichosporium, and uncharacterized environmental enrichments.

  2. Clay enhancement of methane, low molecular weight hydrocarbon and halocarbon conversion by methanotrophic bacteria

    DOE Patents [OSTI]

    Apel, William A.; Dugan, Patrick R.

    1995-04-04

    An apparatus and method for increasing the rate of oxidation of toxic vapors by methanotrophic bacteria. The toxic vapors of interest are methane and trichloroethylene. The apparatus includes a gas phase bioreactor within a closed loop pumping system or a single pass system. The methanotrophic bacteria include Methylomonas methanica, Methylosinus trichosporium, and uncharacterized environmental enrichments.

  3. Live Webcast on Recent Wind Energy Technology Advances

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webcast titled “Recent Wind Technology Advances” on April 16, 2014, from 3:00 to 4:00 p.m. Eastern Standard Time.

  4. iiESI.org is live! | OpenEI Community

    Open Energy Info (EERE)

    live OpenEI maintenance March 8-9, 2013 Research topics related to ESI Prospects for Nuclear Power(Davis 2012) A Framework for the Optimization of Integrated Energy Systems(Jain...

  5. Live Webinar on the Funding Opportunity for Water Power Manufacturing

    Broader source: Energy.gov [DOE]

    On April 23, 2014 from11:00 AM-1:00 PM MDT, the Water Power Program will hold a live webinar to provide information to potential applicants for the Water Power Manufacturing Funding Opportunity...

  6. Acting Biomass Program Manager Dr. Valerie Reed to Host Live...

    Broader source: Energy.gov (indexed) [DOE]

    December 16th, the Energy Department (@energy) will be hosting a live Twitter Q&A on biofuels with Dr. Valerie Reed, Acting Manager of the Biomass Program. Dr. Reed holds a Ph. D....

  7. Spontaneous fission half-lives for ground state nuclides

    SciTech Connect (OSTI)

    Holden, N.E. (Brookhaven National Lab., Upton, NY (United States)); Hoffman, D.C. (Lawrence Berkeley Lab., CA (United States))

    1991-01-01

    Measurements of the spontaneous fission half-lives of nuclides of elements Z = 90 to 107 have been compiled and evaluated. Recommended values are presented. 126 refs., 96 tabs.

  8. Follow Live Dec 24: Los Alamos National Lab Tracks Rudolph's...

    Broader source: Energy.gov (indexed) [DOE]

    www.santa.lanl.gov"> follow along live as Santa circles the globe this Christmas Eve. | Image credit: Hantz Leger. Thanks to the Los Alamos National Laboratory, you can...

  9. EIA - Greenhouse Gas Emissions Overview

    Gasoline and Diesel Fuel Update (EIA)

    A2. Glossary Acid stabilization: A circumstance where the pH of the waste mixture in an animal manure management system is maintained near 7.0, optimal conditions for methane production. Aerobic bacteria: Microorganisms living, active, or occurring only in the presence of oxygen. Aerobic decomposition: The breakdown of a molecule into simpler molecules or atoms by microorganisms under favorable conditions of oxygenation. Aerosols: Airborne particles. Afforestation: Planting of new forests on

  10. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Tracking Living Cells as They Differentiate in Real Time Print Thursday, 27 September 2012 00:00 Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible

  11. Public invited to share living with wildfire stories with BSM

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Public invited to share living with wildfire stories with BSM Public invited to share living with wildfire stories with BSM The exhibit provides an opportunity for people to share their stories about the Las Conchas fire and other wildfires. June 11, 2012 Personal experiences and stories around wildfire are part of a new interactive exhibit at the Bradbury. Personal experiences and stories around wildfire are part of a new interactive exhibit at the Bradbury. Contact Steve Sandoval

  12. Long-Living Polymer Electrolytes | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Living Polymer Electrolytes Long-Living Polymer Electrolytes 2010 DOE Vehicle Technologies and Hydrogen Programs Annual Merit Review and Peer Evaluation Meeting, June 7-11, 2010 -- Washington D.C. PDF icon es094_janke_2010_p.pdf More Documents & Publications Composite Electrolyte to Stabilize Metallic Lithium Anodes Carbon/Sulfur Nanocomposites and Additives for High-Energy Lithium Sulfur Batteries Polymers For Advanced Lithium Batteries

  13. Stent Technology Saves Lives, Creates Jobs | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Stent Technology Saves Lives, Creates Jobs Stent Technology Saves Lives, Creates Jobs May 8, 2012 - 1:00pm Addthis The platinum/chromium alloy used in new coronary stents manufactured by Boston Scientific Corporation was developed by a research team that included metallurgists from NETL. The platinum/chromium alloy used in new coronary stents manufactured by Boston Scientific Corporation was developed by a research team that included metallurgists from NETL. Washington, DC - When people think of

  14. Superior Energy Performance: New Website Live and Ready for Business |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Superior Energy Performance: New Website Live and Ready for Business Superior Energy Performance: New Website Live and Ready for Business January 16, 2014 - 11:17am Addthis Superior Energy Performance(tm) (SEP) announces its new website-a valuable resource for manufacturers who want to prove their achievements in energy management and lower plant energy costs. SEP is a certification program and process applicable to facilities of all sizes in all manufacturing sectors.

  15. Invisible Science: Lab Breakthroughs in Our Daily Lives | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Invisible Science: Lab Breakthroughs in Our Daily Lives Invisible Science: Lab Breakthroughs in Our Daily Lives April 24, 2012 - 2:30pm Addthis The Lab Breakthroughs video series focuses on the array of technological advancements and discoveries that stem from research performed in the National Labs, including improvements in industrial processes, discoveries in fundamental scientific research, and innovative medicines. <a href="http://energy.gov/lab-breakthroughs">See

  16. Live from Greenbuild: From the Industrial Facilities Connect & Learn |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Live from Greenbuild: From the Industrial Facilities Connect & Learn Live from Greenbuild: From the Industrial Facilities Connect & Learn November 18, 2015 - 5:32pm Addthis By Monica Kanojia The industrial industry is filled with unique and dynamic projects with substantially high process loads and resource consumption. This sector faces a different set of challenges in attaining sustainably built campuses. Owners must address compliance, regulations and safety

  17. Living Comfortably: A Consumer's Guide to Home Energy Upgrades |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Living Comfortably: A Consumer's Guide to Home Energy Upgrades Living Comfortably: A Consumer's Guide to Home Energy Upgrades March 7, 2013 - 3:15pm Addthis A weatherization worker drills holes to blow cellulose insulation in the interior walls of this home. | Photo courtesy of Dennis Schroeder, NREL A weatherization worker drills holes to blow cellulose insulation in the interior walls of this home. | Photo courtesy of Dennis Schroeder, NREL Dr. Richard Knaub Project

  18. LiveWall Operational Evaluation: Seattle Law Enforcement Pilot

    SciTech Connect (OSTI)

    Barr, Jonathan L.; Burtner, Edwin R.; Stein, Steven L.

    2013-10-01

    The LiveWall concept envisioned as an outgrowth of the Precision Information Environment (PIE) project allows communications between separate groups using interactive video, audio, and a shared desktop environment; this allows everyone to participate and collaborate in real time, regardless of location. The LiveWall concept provides a virtual window to other locations, where all parties can interact and collaboratively work with each other. This functionality is intended to improve multi-site coordination amongst emergency operations centers (EOC), field operations sites and across organizations and jurisdictions to accommodate communications during routine and emergency events. For the initial LiveWall operational evaluation PNNL partnered with the Seattle Police Department (SPD). This partnership allowed for the creation of an excellent LiveWall test bed specific to law enforcement. This partnership made it possible to test the LiveWall concept with scenarios involving the many facets of the law enforcement work done by SPD. PNNL and SPD agreed that integrating the systems into operations for a real event would be the best test of the technology and give SPD staff greater visibility into the functionality and benefits offered by the LiveWall concept.

  19. Estimating Bacteria Emissions from Inversion of Atmospheric Transport: Sensitivity to Modelled Particle Characteristics

    SciTech Connect (OSTI)

    Burrows, Susannah M.; Rayner, Perter; Butler, T.; Lawrence, M.

    2013-06-04

    Model-simulated transport of atmospheric trace components can be combined with observed concentrations to obtain estimates of ground-based sources using various inversion techniques. These approaches have been applied in the past primarily to obtain source estimates for long-lived trace gases such as CO2. We consider the application of similar techniques to source estimation for atmospheric aerosols, by using as a case study the estimation of bacteria emissions from different ecosystem regions in the global atmospheric chemistry and climate model ECHAM5/MESSy-Atmospheric Chemistry (EMAC). Simulated particle concentrations in the tropopause region and at high latitudes, as well as transport of particles to tundra and land ice regions are shown to be highly sensitive to scavenging in mixed-phase clouds, which is poorly characterized in most global climate models. This may be a critical uncertainty in correctly simulating the transport of aerosol particles to the Arctic. Source estimation via Monte Carlo Markov Chain is applied to a suite of sensitivity simulations and the global mean emissions are estimated. We present an analysis of the partitioning of uncertainties in the global mean emissions that are attributable to particle size, CCN activity, the ice nucleation scavenging ratios for mixed-phase and cold clouds, and measurement error. Uncertainty due to CCN activity or to a 1 um error in particle size is typically between 10% and 40% of the uncertainty due to data uncertainty, as measured by the 5%-ile to 95%-ile range of the Monte Carlo ensemble. Uncertainty attributable to the ice nucleation scavenging ratio in mized-phase clouds is as high as 10% to 20% of the data uncertainty. Taken together, the four model 20 parameters examined contribute about half as much to the uncertainty in the estimated emissions as do the measurements. This was a surprisingly large contribution from model uncertainty in light of the substantial data uncertainty, which ranges from 81% to 870% for each of ten ecosystems for this case study. The effects of these and other model parameters in contributing to the uncertainties in the transport of atmospheric aerosol particles should be treated explicitly and systematically in both forward and inverse modelling studies.

  20. Search for Charged Massive Long-Lived Particles

    SciTech Connect (OSTI)

    Abazov V. M.; Abbott B.; Acharya B. S.; Adams M.; Adams T.; Alexeev G. D.; Alimena J.; Alkhazov G.; Alton A.; Alverson G.; Alves G. A.; Aoki M.; Askew A.; Asman B.; Atkins S.; Atramentov O.; Augsten K.; Avila C.; BackusMayes J.; Badaud F.; Bagby L.; Baldin B.; Bandurin D. V.; Banerjee S.; Barberis E.; Baringer P.; Barreto J.; Bartlett J. F.; Bassler U.; Bazterra V.; Bean A.; Begalli M.; Belanger-Champagne C.; Bellantoni L.; Beri S. B.; Bernardi G.; Bernhard R.; Bertram I.; Besancon M.; Beuselinck R.; Bezzubov V. A.; Bhat P. C.; Bhatnagar V.; Blazey G.; Blessing S.; Bloom K.; Boehnlein A.; Boline D.; Boos E. E.; Borissov G.; Bose T.; Brandt A.; Brandt O.; Brock R.; Brooijmans G.; Bross A.; Brown D.; Brown J.; Bu X. B.; Buehler M.; Buescher V.; Bunichev V.; Burdin S.; Burnett T. H.; Buszello C. P.; Calpas B.; Camacho-Perez E.; Carrasco-Lizarraga M. A.; Casey B. C. K.; Castilla-Valdez H.; Chakrabarti S.; Chakraborty D.; Chan K. M.; Chandra A.; Chapon E.; Chen G.; Chevalier-Thery S.; Cho D. K.; Cho S. W.; Choi S.; Choudhary B.; Cihangir S.; Claes D.; Clutter J.; Cooke M.; Cooper W. E.; Corcoran M.; Couderc F.; Cousinou M. -C.; Croc A.; Cutts D.; Das A.; Davies G.; De K.; de Jong S. J.; De la Cruz-Burelo E.; Deliot F.; Demina R.; Denisov D.; Denisov S. P.; Desai S.; Deterre C.; DeVaughan K.; Diehl H. T.; Diesburg M.; Ding P. F.; Dominguez A.; Dorland T.; Dubey A.; Dudko L. V.; Duggan D.; Duperrin A.; Dutt S.; Dyshkant A.; Eads M.; Edmunds D.; Ellison J.; Elvira V. D.; Enari Y.; Evans H.; Evdokimov A.; Evdokimov V. N.; Facini G.; Ferbel T.; Fiedler F.; Filthaut F.; Fisher W.; Fisk H. E.; Fortner M.; Fox H.; Fuess S.; Garcia-Bellido A.; Garcia-Guerra G. A.; Gavrilov V.; Gay P.; Geng W.; Gerbaudo D.; Gerber C. E.; Gershtein Y.; Ginther G.; Golovanov G.; Goussiou A.; Grannis P. D.; Greder S.; Greenlee H.; Greenwood Z. D.; Gregores E. M.; Grenier G.; Gris Ph.; Grivaz J. -F.; Grohsjean A.; Gruenendahl S.; Gruenewald M. W.; Guillemin T.; Gutierrez G.; Gutierrez P.; Haas A.; Hagopian S.; Haley J.; Han L.; Harder K.; Harel A.; Hauptman J. M.; Hays J.; Head T.; Hebbeker T.; Hedin D.; Hegab H.; Heinson A. P.; Heintz U.; Hensel C.; Heredia-De La Cruz I.; Herner K.; Hesketh G.; Hildreth M. D.; Hirosky R.; Hoang T.; Hobbs J. D.; Hoeneisen B.; Hohlfeld M.; Hubacek Z.; Hynek V.; Iashvili I.; Ilchenko Y.; Illingworth R.; Ito A. S.; Jabeen S.; Jaffre M.; Jamin D.; Jayasinghe A.; Jesik R.; Johns K.; Johnson M.; Jonckheere A.; Jonsson P.; Joshi J.; Jung A. W.; Juste A.; Kaadze K.; Kajfasz E.; Karmanov D.; Kasper P. A.; Katsanos I.; Kehoe R.; Kermiche S.; Khalatyan N.; Khanov A.; Kharchilava A.; Kharzheev Y. N.; Kohli J. M.; Kozelov A. V.; Kraus J.; Kulikov S.; Kumar A.; Kupco A.; Kurca T.; Kuzmin V. A.; Kvita J.; Lammers S.; Landsberg G.; Lebrun P.; Lee H. S.; Lee S. W.; Lee W. M.; Lellouch J.; Li L.; Li Q. Z.; Lietti S. M.; Lim J. K.; Lincoln D.; Linnemann J.; Lipaev V. V.; Lipton R.; Liu Y.; Lobodenko A.; Lokajicek M.; de Sa R. Lopes; Lubatti H. J.; Luna-Garcia R.; Lyon A. L.; Maciel A. K. A.; Mackin D.; Madar R.; Magana-Villalba R.; Malik S.; Malyshev V. L.; Maravin Y.; Martinez-Ortega J.; McCarthy R.; McGivern C. L.; Meijer M. M.; et al.

    2012-03-21

    We report on a search for charged massive long-lived particles (CMLLPs), based on 5.2 fb{sup -1} of integrated luminosity collected with the D0 detector at the Fermilab Tevatron p{bar p} collider. We search for events in which one or more particles are reconstructed as muons but have speed and ionization energy loss (dE/dx) inconsistent with muons produced in beam collisions. CMLLPs are predicted in several theories of physics beyond the standard model. We exclude pair-produced long-lived gaugino-like charginos below 267 GeV and Higgsino-like charginos below 217 GeV at 95% C.L., as well as long-lived scalar top quarks with mass below 285 GeV.

  1. Amoebae/bacteria consortia and uses for degrading wastes and contaminants

    DOE Patents [OSTI]

    Tyndall, Richard L. (Clinton, TN)

    1996-01-01

    A method of altering trinitrotoluene includes the steps of: providing an amoeba/bacteria consortium, particularly ATCC 40908 or a mutant thereof possessing all the identifying characteristics thereof; and contacting the consortium with trinitrotoluene to alter the trinitrotoluene.

  2. Discovery of functional toxin/antitoxin systems in bacteria by shotgun

    Office of Scientific and Technical Information (OSTI)

    cloning (Journal Article) | SciTech Connect Discovery of functional toxin/antitoxin systems in bacteria by shotgun cloning Citation Details In-Document Search Title: Discovery of functional toxin/antitoxin systems in bacteria by shotgun cloning Toxin-antitoxin (TA) modules, composed of a toxic protein and a counteracting antitoxin, play important roles in bacterial physiology. We examined the experimental insertion of 1.5 million genes from 388 microbial genomes into an Escherichia coli host

  3. Purple Bacteria Develops Its Own Form of Sunscreen | U.S. DOE Office of

    Office of Science (SC) Website

    Science (SC) Purple Bacteria Develops Its Own Form of "Sunscreen" Energy Frontier Research Centers (EFRCs) EFRCs Home Centers Research Science Highlights Highlight Archives News & Events Publications History Contact BES Home 05.03.12 Purple Bacteria Develops Its Own Form of "Sunscreen" Print Text Size: A A A FeedbackShare Page Scientific Achievement Found that specific pigments in the light harvesting complex of a photosynthetic bacterium act primarily to protect the

  4. Toxin-eating bacteria and bioremediation (Patent) | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Toxin-eating bacteria and bioremediation Citation Details In-Document Search Title: Toxin-eating bacteria and bioremediation Methods are provided for reducing a level of one or more antibiotics from an antibiotic-contaminated substance. An organism that can utilize the one or more antibiotics as a carbon source is cultured in the presence of the antibiotic-contaminated substance for a sufficient amount of time to reduce the level of one or more antibiotics from the antibiotic-contaminated

  5. Xylan utilization in human gut commensal bacteria is orchestrated by unique

    Office of Scientific and Technical Information (OSTI)

    modular organization of polysaccharide-degrading enzymes (Journal Article) | SciTech Connect Xylan utilization in human gut commensal bacteria is orchestrated by unique modular organization of polysaccharide-degrading enzymes Citation Details In-Document Search Title: Xylan utilization in human gut commensal bacteria is orchestrated by unique modular organization of polysaccharide-degrading enzymes Authors: Zhang, Meiling ; Chekan, Jonathan R. ; Dodd, Dylan ; Hong, Pei-Ying ; Radlinski,

  6. Secretary Chu Speaks at Washington Post Live | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Washington Post Live Secretary Chu Speaks at Washington Post Live November 3, 2011 - 9:12am Addthis Secretary Chu Secretary Chu Former Secretary of Energy Secretary Chu's remarks, as prepared for delivery, at the Washington Post Smart Energy Conference. Thank you, Mary [Jordan], for that kind introduction. While the focus of this conference is on the future of energy, I want to start with some lessons from America's past. On a windy day at Kitty Hawk on December 17, 1903, the Wright Brothers

  7. Table HC1.2.1. Living Space Characteristics by

    U.S. Energy Information Administration (EIA) Indexed Site

    1. Living Space Characteristics by" " Total, Heated, and Cooled Floorspace, 2005" ,,,"Total Square Footage" ,"Housing Units",,"Total1",,"Heated",,"Cooled" "Living Space Characteristics","Millions","Percent","Billions","Percent","Billions","Percent","Billions","Percent" "Total",111.1,100,225.8,100,179.8,100,114.5,100 "Total

  8. How Will We Live Forever? | GE Global Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Live Forever? Click to email this to a friend (Opens in new window) Share on Facebook (Opens in new window) Click to share (Opens in new window) Click to share on LinkedIn (Opens in new window) Click to share on Tumblr (Opens in new window) Invention Factory: How Will We Live Forever? In this episode of Invention Factory - a partnership between GE and Vice - we probe the cutting edge of medical technology that is making the world a healthier place. From cutting-edge CT scanners, to human genome

  9. Living well, doing good | Y-12 National Security Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Living well, doing good Living well, doing good Posted: June 24, 2015 - 3:22pm Print version Joe Marshall is one of Y-12's cancer survivors who took his victory lap at the Anderson County Relay for Life. What do relays, races and regattas have in common? They're all ways Y-12 employees stay active and support the community. After hours, Y-12ers are all about staying active and supporting their communities. Here's a roundup of the activities Relay for Life, May 16 For numerous years, Joe Kato and

  10. Live Chat with the EERE Information Center | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Live Chat with the EERE Information Center Live Chat with the EERE Information Center December 6, 2010 - 6:30am Addthis Amy Foster Parish If you're an Energy Savers blog reader, hopefully you're also taking the opportunity to comment on the posts you read. Not only do the Energy Savers bloggers love to hear your feedback and answer your questions, commenting on the posts gives you a great opportunity to interact online with other folks who are really interested in energy efficiency and renewable

  11. Zombie Replicants to Outperform the Living | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Zombie Replicants to Outperform the Living Zombie Replicants to Outperform the Living February 8, 2013 - 5:08pm Addthis The first stage of the "zombie cell" only moderately heated, the cell is now pure silica and needed a gold coating for a scanning electron microscope to image it. | Photo courtesy of Sandia National Laboratories The first stage of the "zombie cell" only moderately heated, the cell is now pure silica and needed a gold coating for a scanning electron

  12. Interested in Open Energy Data? Watch the Energy Datapalooza Live! |

    Office of Environmental Management (EM)

    Department of Energy Interested in Open Energy Data? Watch the Energy Datapalooza Live! Interested in Open Energy Data? Watch the Energy Datapalooza Live! September 27, 2012 - 5:25pm Addthis U.S. Chief Technology Officer Todd Park kicks off the Safety Datapalooza on September 19th. | Photo Courtesy U.S. Department of Labor U.S. Chief Technology Officer Todd Park kicks off the Safety Datapalooza on September 19th. | Photo Courtesy U.S. Department of Labor Matthew Loveless Matthew Loveless

  13. Lab Breakthrough: How Energy Department Research Saves Lives | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy How Energy Department Research Saves Lives Lab Breakthrough: How Energy Department Research Saves Lives August 28, 2013 - 12:50pm Addthis Researchers at the National Energy Technology Lab have developed a platinum-chromium alloy that is used to make heart stents that are thin, flexible, corrosion resistant and visible on x-rays. Since their introduction in 2010, coronary stents made from the new alloy have generated more than $4 billion in worldwide sales and captured 45 percent of

  14. Live Discussion on Energy 101: Fuel Cells | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Live Discussion on Energy 101: Fuel Cells Live Discussion on Energy 101: Fuel Cells January 16, 2014 - 3:59pm Addthis Rebecca Matulka Rebecca Matulka Former Digital Communications Specialist, Office of Public Affairs Editor's Note: Thanks to everyone who participated in our Google+ Hangout on Energy 101: Fuel Cells. We got a lot of great questions, and our experts talked about everything from the future of fuel cell vehicles and how they're being used as backup power to the efficiency benefits

  15. Lead in human blood from children living in Campania, Italy

    SciTech Connect (OSTI)

    Amodio-Cocchieri, R.; Arnese, A.; Prospero, E.; Roncioni, A.

    1996-03-01

    Blood lead (PbB) levels were determined in children living Campania (in Naples and in a rural zone in the district of Caserta). Atmospheric lead (PbA) concentration in these considered areas was monitored for 1 yr (1993-1994). The children tested were questioned about common sources of lead, other than atmospheric relating to their living and dietary habits. The PbB levels in children living in Naples were at the 50th percentile, 18.8 {mu}g/dl in males and 13.7 {mu}g/dl in females; in children living in the rural area the median PbB levels were 8.9{mu}g/dl in males, and 9.9 {mu}g/dl in females. The annual mean values of atmospheres lead were 1.15 {plus_minus} 0.24 {mu}g/dl in Naples and 0.23 {plus_minus} 0.07 {mu}g/dl in the rural area. Significant and congruent mean differences between urban and rural sites were found in children`s blood and concurrent air lead. Considering the PbB level of 10 {mu}g/dl as the maximum level that is not associated any known adverse effect in children, the Neapolitan group can be considered at risk of chronic intoxication by lead. 18 refs., 3 figs., 4 tabs.

  16. Have You Visited the Living Zero Home Tour?

    Broader source: Energy.gov [DOE]

    The Living Zero Home Tour is on the road, and it may be in a city near you! If you have the chance to see the tour, you may be surprised to discover the small steps that you can take to save energy...

  17. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible to watch phosphorylation at the molecular level without damaging cells or interfering with

  18. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible to watch phosphorylation at the molecular level without damaging cells or interfering with

  19. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible to watch phosphorylation at the molecular level without damaging cells or interfering with

  20. The secret lives of scientists & engineers: Matt Kwiatkowski | Argonne

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    National Laboratory This article was published in the spring 2016 issue of Argonne Now, the laboratory science magazine. Click for the rest of the issue. The secret lives of scientists & engineers: Matt Kwiatkowski March 7, 2016 Tweet EmailPrint This article was originally published in the spring 2016 issue of Argonne Now, the laboratory's science magazine. In this series, meet researchers from Argonne with unusual hobbies and interests. Today we're interviewing Matt Kwiatkowski, cyber

  1. The problem of living in a world contaminated with chemicals

    SciTech Connect (OSTI)

    Metcalf, R.L.

    1990-12-31

    The proliferation of xenobiotic chemicals in the global environment poses living problems for each of us aboard {open_quotes}spaceship earth.{close_quotes} Seven case studies are presented that illustrate the magnitude of the problem that can result from waiting to identify toxic hazards until there have been decades of {open_quotes}human guinea pig{close_quotes} exposure. 25 refs., 5 tabs.

  2. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible to watch phosphorylation at the molecular level without damaging cells or interfering with

  3. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tracking Living Cells as They Differentiate in Real Time Print Protein phosphorylation regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to differentiation to metabolism to signaling, and even programmed cell death. This chemical process has been studied intensively, but until now it has been impossible to watch phosphorylation at the molecular level without damaging cells or interfering with

  4. ClimateChangeLIVE Webcast: Join the Climate Conversation

    Broader source: Energy.gov [DOE]

    Join ClimateChangeLIVE's webcast, bringing together students and climate experts for a discussion about climate change and what students and classes around the country are doing to be part of the climate solution. Students will be able to interact with climate scientists and experts online through Facebook and Twitter. A GreenWorks! grant will be offered to help schools with climate action projects.

  5. Single-photon ultrashort-lived radionuclides: symposium proceedings

    SciTech Connect (OSTI)

    Paras, P.; Thiessen, J.W. (eds.)

    1985-01-01

    The purpose was to define the current role and state-of-the-art regarding the development, clinical applications, and usefulness of generator-produced single-photon ultrashort-lived radionuclides (SPUSLR's) and to predict their future impact on medicine. Special emphasis was placed on the generator production of iridium-191, gold-195, and krypton-81. This report contains expanded summaries of the included papers. (ACR)

  6. Live from the Lab - SPORT Video | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Live from the Lab - SPORT Video Thanks to the innovation of "single particle orientation and rotation tracking" (SPORT), we now can watch the distinctive movements of drug delivering nanoparticles in real time. Nanoparticles have the potential to revolutionize drug delivery. When these particles interact with cell membranes they move in all sorts of ways. They spin, they tumble, they move along and through the membrane. At least that's what we think. But what's really going on? Until

  7. Live from the Clinton Global Initiative | Department of Energy

    Office of Environmental Management (EM)

    from the Clinton Global Initiative Live from the Clinton Global Initiative September 21, 2010 - 1:00pm Addthis John Schueler John Schueler Former New Media Specialist, Office of Public Affairs What does this project do? We're partnering with the Clinton Global Initiative and other NGOs to bring clean-burning cookstoves to the developing world. Today Secretary of State Hillary Rodham Clinton formally announced the Global Alliance for Clean Cookstoves, a new public-private initiative to create a

  8. Liquid Fuel From Renewable Electricity and Bacteria: Electro-Autotrophic Synthesis of Higher Alcohols

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: UCLA is utilizing renewable electricity to power direct liquid fuel production in genetically engineered Ralstonia eutropha bacteria. UCLA is using renewable electricity to convert carbon dioxide into formic acid, a liquid soluble compound that delivers both carbon and energy to the bacteria. The bacteriaare genetically engineered to convert the formic acid into liquid fuelin this case alcohols such as butanol. The electricity required for the process can be generated from sunlight, wind, or other renewable energy sources. In fact, UCLAs electricity-to-fuel system could be a more efficient way to utilize these renewable energy sources considering the energy density of liquid fuel is much higher than the energy density of other renewable energy storage options, such as batteries.

  9. Genetically Modified Bacteria for Fuel Production: Development of Rhodobacteria as a Versatile Platform for Fuels Production

    SciTech Connect (OSTI)

    2010-07-01

    Electrofuels Project: Penn State is genetically engineering bacteria called Rhodobacter to use electricity or electrically generated hydrogen to convert carbon dioxide into liquid fuels. Penn State is taking genes from oil-producing algae called Botryococcus braunii and putting them into Rhodobacter to produce hydrocarbon molecules, which closely resemble gasoline. Penn State is developing engineered tanks to support microbial fuel production and determining the most economical way to feed the electricity or hydrogen to the bacteria, including using renewable sources of power like solar energy.

  10. Geek-Up[12.03.2010]: Halomonadaceae Bacteria and the Return of Quark Gluon

    Energy Savers [EERE]

    Plasma | Department of Energy 03.2010]: Halomonadaceae Bacteria and the Return of Quark Gluon Plasma Geek-Up[12.03.2010]: Halomonadaceae Bacteria and the Return of Quark Gluon Plasma December 3, 2010 - 4:59pm Addthis Niketa Kumar Niketa Kumar Public Affairs Specialist, Office of Public Affairs As a fellow geek, you probably heard NASA's big news yesterday. If not, here's a recap: It was once assumed that all life on Earth, from humans and giraffes to scorpions and plankton, are all based on

  11. Non-destructive observation of intact bacteria and viruses in water by the highly sensitive frequency transmission electric-field method based on SEM

    SciTech Connect (OSTI)

    Ogura, Toshihiko

    2014-08-08

    Highlights: We developed a high-sensitive frequency transmission electric-field (FTE) system. The output signal was highly enhanced by applying voltage to a metal layer on SiN. The spatial resolution of new FTE method is 41 nm. New FTE system enables observation of the intact bacteria and virus in water. - Abstract: The high-resolution structural analysis of biological specimens by scanning electron microscopy (SEM) presents several advantages. Until now, wet bacterial specimens have been examined using atmospheric sample holders. However, images of unstained specimens in water using these holders exhibit very poor contrast and heavy radiation damage. Recently, we developed the frequency transmission electric-field (FTE) method, which facilitates the SEM observation of biological specimens in water without radiation damage. However, the signal detection system presents low sensitivity. Therefore, a high EB current is required to generate clear images, and thus reducing spatial resolution and inducing thermal damage to the samples. Here a high-sensitivity detection system is developed for the FTE method, which enhances the output signal amplitude by hundredfold. The detection signal was highly enhanced when voltage was applied to the metal layer on silicon nitride thin film. This enhancement reduced the EB current and improved the spatial resolution as well as the signal-to-noise ratio. The spatial resolution of a high-sensitive FTE system is 41 nm, which is considerably higher than previous FTE system. New FTE system can easily be utilised to examine various unstained biological specimens in water, such as living bacteria and viruses.

  12. Genomics Encyclopedia of Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB): a resource for microsymbiont genomes (2013 DOE JGI Genomics of Energy and Environment 8th Annual User Meeting)

    SciTech Connect (OSTI)

    Reeve, Wayne [Murdoch University

    2013-03-01

    Wayne Reeve of Murdoch University on "Genomics Encyclopedia of Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB): a resource for microsymbiont genomes" at the 8th Annual Genomics of Energy & Environment Meeting on March 27, 2013 in Walnut Creek, Calif.

  13. Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals

    DOE Patents [OSTI]

    Gambhir, Sanjiv; Pritha, Ray

    2015-07-14

    Novel double and triple fusion reporter gene constructs harboring distinct imagable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

  14. Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals

    DOE Patents [OSTI]

    Gambhir; Sanjiv (Portola Valley, CA), Pritha; Ray (Mountain View, CA)

    2009-04-28

    Novel double and triple fusion reporter gene constructs harboring distinct imageable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

  15. Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals

    DOE Patents [OSTI]

    Gambhir, Sanjiv (Portola Valley, CA); Pritha, Ray (Mountain View, CA)

    2011-06-07

    Novel double and triple fusion reporter gene constructs harboring distinct imagable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

  16. ScienceLive chat page: on the future of fusion research | Princeton Plasma

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Physics Lab ScienceLive chat page: on the future of fusion research American Fusion News Category: U.S. Universities Link: ScienceLive chat page: on the future of fusion research

  17. TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's Oil Dependence Through Innovation TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's ...

  18. #AskEnergy: Live Twitter Q&A on Solar Energy | Department of...

    Energy Savers [EERE]

    Live Twitter Q&A on Solar Energy AskEnergy: Live Twitter Q&A on Solar Energy February 10, 2014 - 10:35am Addthis What do you want to know about solar energy? Submit your questions ...

  19. WAC - 232-12-064 Live Wildlife-Taking from the wild | Open Energy...

    Open Energy Info (EERE)

    32-12-064 Live Wildlife-Taking from the wild Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: WAC - 232-12-064 Live...

  20. Amoebae/bacteria consortia and uses for degrading wastes and contaminants

    DOE Patents [OSTI]

    Tyndall, R.L.

    1996-05-21

    A method is disclosed of altering trinitrotoluene. The steps include the following: providing an amoeba/bacteria consortium, particularly ATCC 40908 or a mutant which possesses all the identifying characteristics thereof; and contacting the consortium with trinitrotoluene to alter the trinitrotoluene.

  1. Methanobactin: a copper binding compound having antibiotic and antioxidant activity isolated from methanotrophic bacteria

    DOE Patents [OSTI]

    DiSpirito, Alan A. (Ames, IA); Zahn, James A. (Harbor Beach, MI); Graham, David W. (Lawrence, KS); Kim, Hyung J. (St. Paul, MN); Alterman, Michail (Lawrence, KS); Larive, Cynthia (Lawrence, KS)

    2007-04-03

    A means and method for treating bacterial infection, providing antioxidant activity, and chelating copper using a copper binding compound produced by methanotrophic bacteria is described. The compound, known as methanobactin, is the first of a new class of antibiotics having gram-positive activity. Methanobactin has been sequenced, and its structural formula determined.

  2. Wendee Brunish-So that others may live

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Wendee Brunish Wendee Brunish-So that others may live At work, this Lab astrophysicist spends long hours searching the planet for concealed weapons of mass destruction. March 10, 2014 Wendee Brunish At work, this Lab astrophysicist spends long hours searching the planet for concealed weapons of mass destruction. After hours-and often for days in dangerous conditions-she's searching steep, heavily wooded mountains for missing persons. "I am most proud of the fact that I am one of the few

  3. Ask a scientist: Nanotech in our lives | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    spring 2014 issue of Argonne Now, the laboratory science magazine. Ask a scientist: Nanotech in our lives June 1, 2014 Tweet EmailPrint Is there nanotechnology already in my consumer products? Carrado Gregar: I just saw a report that named 1,628 products using nanotechnology...so I'd say yes, definitely! When you say nanotech, a lot of people are thinking of tiny machines or robots. There is a long way to go before that becomes reality. But simpler nanomaterials are very much in common use.

  4. Making the "Best Place to Live" Even Better

    Broader source: Energy.gov [DOE]

    For 2010, the appropriately named Eden Prairie, Minnesota was honored as the No. 1 Best Place to Live in the United States by Money Magazine. The highly-coveted civic title reflects many aspects of the family-friendly suburb of 62,000 located 16 miles from downtown Minneapolis, including the advantages of being the home to major employers like Fortune 500 trucking company C.H. Robinson and hearing-aid maker Starkey Labs. The city also hass many natural amenities like 17 lakes and parks with 125 miles of running, hiking, and biking trails.

  5. Rapid quantification of mutant fitness in diverse bacteria by sequencing randomly bar-coded transposons

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wetmore, Kelly M.; Price, Morgan N.; Waters, Robert J.; Lamson, Jacob S.; He, Jennifer; Hoover, Cindi A.; Blow, Matthew J.; Bristow, James; Butland, Gareth; Arkin, Adam P.; et al

    2015-05-12

    Transposon mutagenesis with next-generation sequencing (TnSeq) is a powerful approach to annotate gene function in bacteria, but existing protocols for TnSeq require laborious preparation of every sample before sequencing. Thus, the existing protocols are not amenable to the throughput necessary to identify phenotypes and functions for the majority of genes in diverse bacteria. Here, we present a method, random bar code transposon-site sequencing (RB-TnSeq), which increases the throughput of mutant fitness profiling by incorporating random DNA bar codes into Tn5 and mariner transposons and by using bar code sequencing (BarSeq) to assay mutant fitness. RB-TnSeq can be used with anymore » transposon, and TnSeq is performed once per organism instead of once per sample. Each BarSeq assay requires only a simple PCR, and 48 to 96 samples can be sequenced on one lane of an Illumina HiSeq system. We demonstrate the reproducibility and biological significance of RB-TnSeq with Escherichia coli, Phaeobacter inhibens, Pseudomonas stutzeri, Shewanella amazonensis, and Shewanella oneidensis. To demonstrate the increased throughput of RB-TnSeq, we performed 387 successful genome-wide mutant fitness assays representing 130 different bacterium-carbon source combinations and identified 5,196 genes with significant phenotypes across the five bacteria. In P. inhibens, we used our mutant fitness data to identify genes important for the utilization of diverse carbon substrates, including a putative D-mannose isomerase that is required for mannitol catabolism. RB-TnSeq will enable the cost-effective functional annotation of diverse bacteria using mutant fitness profiling. A large challenge in microbiology is the functional assessment of the millions of uncharacterized genes identified by genome sequencing. Transposon mutagenesis coupled to next-generation sequencing (TnSeq) is a powerful approach to assign phenotypes and functions to genes. However, the current strategies for TnSeq are too laborious to be applied to hundreds of experimental conditions across multiple bacteria. Here, we describe an approach, random bar code transposon-site sequencing (RB-TnSeq), which greatly simplifies the measurement of gene fitness by using bar code sequencing (BarSeq) to monitor the abundance of mutants. We performed 387 genome-wide fitness assays across five bacteria and identified phenotypes for over 5,000 genes. RB-TnSeq can be applied to diverse bacteria and is a powerful tool to annotate uncharacterized genes using phenotype data.« less

  6. From: Meredith Brown <racer@lanl.gov> Subject: Green Alert: VPP Program Saves Lives

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    24 Feb 1998 09:44:53 -0700 From: Meredith Brown <racer@lanl.gov> Subject: Green Alert: VPP Program Saves Lives Title: GREEN - VPP Program Saves Lives Identifier: INEEL Lessons Learned #98002 Date: January 8, 1998 Lessons Learned: The proactive safety practices and actively caring attitudes that are a part of DOE's VPP Program are designed to extend beyond the workplace into employees' personal lives. The positive behavioral changes can save lives. Summary of Success Story: An employee

  7. DOE Tour of Zero Floorplans: Options for Community Living by United Way of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Long Island | Department of Energy Options for Community Living by United Way of Long Island DOE Tour of Zero Floorplans: Options for Community Living by United Way of Long Island DOE Tour of Zero Floorplans: Options for Community Living by United Way of Long Island

  8. Live wires: direct extracellular electron exchange for bioenergy and the bioremediation of energy-related contamination

    SciTech Connect (OSTI)

    Lovley, DR

    2011-12-01

    Microorganisms that can form direct electrical connections with insoluble minerals, electrodes, or other microorganisms can play an important role in some traditional as well as novel bioenergy strategies and can be helpful in the remediation of environmental contamination resulting from the use of more traditional energy sources. The surprising discovery that microorganisms in the genus Geobacter are capable of forming highly conductive networks of filaments that transfer electrons along their length with organic metallic-like conductivity, rather than traditional molecule to molecule electron exchange, provides an explanation for the ability of Geobacter species to grow in subsurface environments with insoluble Fe(III) oxides as the electron acceptor, and effectively remediate groundwater contaminated with hydrocarbon fuels or uranium and similar contaminants associated with the mining and processing of nuclear fuel. A similar organic metallic-like conductivity may be an important mechanism for microorganisms to exchange electrons in syntrophic associations, such as those responsible for the conversion of organic wastes to methane in anaerobic digesters, a proven bioenergy technology. Biofilms with conductivities rivaling those of synthetic polymers help Geobacter species generate the high current densities in microbial fuel cells producing electric current from organic compounds. Electron transfer in the reverse direction, i.e. from electrodes to microbes, is the basis for microbial electrosynthesis, in which microorganisms reduce carbon dioxide to fuels and other useful organic compounds with solar energy in a form of artificial photosynthesis that is more efficient and avoids many of the environmental sustainability concerns associated with biomass-based bioenergy strategies. The ability of Geobacter species to produce highly conductive electronic networks that function in water opens new possibilities in the emerging field of bioelectronics.

  9. Air Leakage and Air Transfer Between Garage and Living Space

    SciTech Connect (OSTI)

    Rudd, Armin

    2014-09-01

    This research project focused on evaluation of air transfer between the garage and living space in a single-family detached home constructed by a production homebuilder in compliance with the 2009 International Residential Code and the 2009 International Energy Conservation Code. The project gathered important information about the performance of whole-building ventilation systems and garage ventilation systems as they relate to minimizing flow of contaminated air from garage to living space. A series of 25 multi-point fan pressurization tests and additional zone pressure diagnostic testing characterized the garage and house air leakage, the garage-to-house air leakage, and garage and house pressure relationships to each other and to outdoors using automated fan pressurization and pressure monitoring techniques. While the relative characteristics of this house may not represent the entire population of new construction configurations and air tightness levels (house and garage) throughout the country, the technical approach was conservative and should reasonably extend the usefulness of the results to a large spectrum of house configurations from this set of parametric tests in this one house. Based on the results of this testing, the two-step garage-to-house air leakage test protocol described above is recommended where whole-house exhaust ventilation is employed.

  10. From Nanowires to Biofilms: An Exploration of Novel Mechanisms of Uranium Transformation Mediated by Geobacter Bacteria

    SciTech Connect (OSTI)

    REGUERA, GEMMA

    2014-01-16

    One promising strategy for the in situ bioremediation of radioactive groundwater contaminants that has been identified by the SBR Program is to stimulate the activity of dissimilatory metal-reducing microorganisms to reductively precipitate uranium and other soluble toxic metals. The reduction of U(VI) and other soluble contaminants by Geobacteraceae is directly dependent on the reduction of Fe(III) oxides, their natural electron acceptor, a process that requires the expression of Geobacters conductive pili (pilus nanowires). Expression of conductive pili by Geobacter cells leads to biofilm development on surfaces and to the formation of suspended biogranules, which may be physiological closer to biofilms than to planktonic cells. Biofilm development is often assumed in the subsurface, particularly at the matrix-well screen interface, but evidence of biofilms in the bulk aquifer matrix is scarce. Our preliminary results suggest, however, that biofilms develop in the subsurface and contribute to uranium transformations via sorption and reductive mechanisms. In this project we elucidated the mechanism(s) for uranium immobilization mediated by Geobacter biofilms and identified molecular markers to investigate if biofilm development is happening in the contaminated subsurface. The results provided novel insights needed in order to understand the metabolic potential and physiology of microorganisms with a known role in contaminant transformation in situ, thus having a significant positive impact in the SBR Program and providing novel concept to monitor, model, and predict biological behavior during in situ treatments.

  11. WATCH LIVE: Talking the Higgs Boson with CERN | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    WATCH LIVE: Talking the Higgs Boson with CERN WATCH LIVE: Talking the Higgs Boson with CERN September 13, 2012 - 2:33pm Addthis WATCH LIVE: Talking the Higgs Boson with CERN Michael Hess Michael Hess Former Digital Communications Specialist, Office of Public Affairs Where do I watch? To watch this livestream, go to energy.gov/live. Tune in to a special livestream at energy.gov/live on Friday, Sept. 14 at 10 a.m. ET for a science lecture about the Higgs boson with Joe Incandela, head spokesperson

  12. Firearm equipped with live round inhibiting means and method of making same

    DOE Patents [OSTI]

    Baehr, D.G.

    1990-11-13

    A firearm is disclosed having live round inhibiting means mounted in the barrel of the firearm which permits a blank cartridge to be loaded into a firearm and fired while preventing the loading and firing of a live round. The live round inhibiting means comprise shaft means mounted in the barrel of the firearm and which extends a sufficient length into the barrel at a point just beyond the chamber portion of the firearm to engage the bullet portion of a live round to prevent it from properly chambering, while permitting a blank cartridge to be loaded into the firearm and fired without engaging the live round-inhibiting shaft means. 9 figs.

  13. Firearm equipped with live round inhibiting means and method of making same

    DOE Patents [OSTI]

    Baehr, Donald G. (Albuquerque, NM)

    1990-01-01

    A firearm is disclosed having live round inhibiting means mounted in the barrel of the firearm which permits a blank cartridge to be loaded into a firearm and fired while preventing the loading and firing of a live round. The live round inhibiting means comprise shaft means mounted in the barrel of the firearm and which extends a sufficient length into the barrel at a point just beyond the chamber portion of the firearm to engage the bullet portion of a live round to prevent it from properly chambering, while permitting a blank cartridge to be loaded into the firearm and fired without engaging the live round-inhibiting shaft means.

  14. South Atlantic OCS area living marine resources study. Volume I: an investigation of live bottom habitats south of Cape Fear, North Carolina. Final report

    SciTech Connect (OSTI)

    Not Available

    1981-10-01

    The major objectives of this study were to (1) characterize benthic and nektonic communities associated with representative live bottom habitats on the continental shelf of the South Atlantic Bight, and (2) evaluate factors which might influence these communities, particularly the potential for impact by offshore oil and gas activities. The study areas include nine live bottom areas located off South Carolina, Georgia and Florida.

  15. Novel microorganism for selective separation of coal from ash and pyrite. Second quarterly technical progress report, 1 December 1993--28 February 1994

    SciTech Connect (OSTI)

    Misra, M.; Smith, R.W.; Raichur, A.M.

    1994-05-01

    The objective of this project is to study the effectiveness of a novel hydrophobic microorganism, Mycobacterium phlei (M. phlei), for the selective flocculation of coal from pyrite and ash forming minerals. During the reporting period, the hydrophobicity of different coal samples was studied both in the presence and absence of M. phlei cells. In the absence of M. Phlei, Illinois No. 6 and Pennsylvania No. 8 exhibited higher contact angles as compared to Kentucky No. 9 coal. All the coal samples exhibited a maximum in contact angle around pH 5--7, which roughly coincides with the iso-electric point (iep) of different coals studied in this investigation. In the presence of M. phlei, maximum contact angle shifted to lower pH range of 2--3 which coincides with the iep of the M. phlei. These measurements reinforce the notion that good flocculation of coal with M. phlei can be achieved around pH 2--3. The amount of soluble fraction released during rupturing of M. phlei cells was studied as a function of sonication time. The rupturing experiments showed that the whole cells (unruptured cells) contain nearly 40% by weight of soluble fractions. Also, during the reporting period, the fabrication of the counter-current flocculation device was completed.

  16. Experimental test of whether electrostatically charged micro-organisms and their spores contribute to the onset of arcs across vacuum gaps

    SciTech Connect (OSTI)

    Grisham, L. R.; Halle, A. von; Carpe, A. F.; Gilton, K. R.; Rossi, Guy; Stevenson, T. N.

    2013-12-15

    Recently it was proposed [L. R. Grisham et al. Phys. Plasmas 19, 023107 (2012)] that one of the initiators of vacuum voltage breakdown between conducting electrodes might be micro-organisms and their spores, previously deposited during exposure to air, which then become electrostatically charged when an electric potential is applied across the vacuum gap. This note describes a simple experiment to compare the number of voltage-conditioning pulses required to reach the nominal maximum operating voltage across a gap between two metallic conductors in a vacuum, comparing cases in which biological cleaning was done just prior to pump-down with cases where this was not done, with each case preceded by exposure to ambient air for three days. Based upon these results, it does not appear that air-deposited microbes and their spores constitute a major pathway for arc initiation, at least for exposure periods of a few days, and for vacuum gaps of a few millimeters, in the regime where voltage holding is usually observed to vary linearly with gap distance.

  17. Experimental Test Of Whether Electrostatically Charged Micro-organisms And Their Spores Contribute To The Onset Of Arcs Across Vacuum Gaps

    SciTech Connect (OSTI)

    none,; Grisham, Larry R.

    2014-02-24

    Recently it was proposed [L.R. Grisham, A. vonHalle, A.F. Carpe, Guy Rossi, K.R. Gilton, E.D. McBride, E.P. Gilson, A. Stepanov, T.N. Stevenson, Physics of Plasma 19 023107 (2012)] that one of the initiators of vacuum voltage breakdown between condu cting electrodes might be micro-organisms and their spores, previously deposited during exposure to air, which tnen become electrostatically charged when an electric potential is applied across the vacuum gap. The note describes a simple experiment to compare the number of voltage-conditioning pulses required to reach the nominal maxium operating voltage across a gap between two metallic conductors in a vacuum, comparing cases in which biological cleaning was done just prior to pump-down with cases where this was not done, with each preceded by exposure to ambient air for three days. Based upon these results, it does not appear that air-deposited microbes and their spores constitute a major pathway for arc initiation, at least for exposure periods of a few days, and for vacuum gaps of a few millimeters, in the regime where voltage holding is usually observed to vary linearly with gap distance

  18. Near-Term Climate Mitigation by Short-Lived Forcers

    SciTech Connect (OSTI)

    Smith, Steven J.; Mizrahi, Andrew H.

    2013-08-12

    Emissions reductions focused on anthropogenic climate forcing agents with relatively short atmospheric lifetimes such as methane (CH4) and black carbon (BC) have been suggested as a strategy to reduce the rate of climate change over the next several decades. We find that reductions of methane and BC would likely have only a modest impact on near-term climate warming. Even with maximally feasible reductions phased in from 2015 to 2035, global mean temperatures in 2050 are reduced by 0.16 C, with an uncertainty range of 0.04-0.36C, with the high end of this range only possible if total historical aerosol forcing is small. More realistic mitigation scenarios would likely provide a smaller climate benefit. The climate benefits from targeted reductions in short-lived forcing agents are smaller than previously estimated and are not substantially different in magnitude from the benefits due to a comprehensive climate policy.

  19. Short-lived isomers in {sup 94}Rb

    SciTech Connect (OSTI)

    Tsekhanovich, I.; Dare, J. A.; Smith, A. G.; Varley, B. J.; Simpson, G. S.; Urban, W.; Soldner, T.; Jolie, J.; Linnemann, A.; Orlandi, R.; Smith, J. F.; Scherillo, A.; Rzaca-Urban, T.; Zlomaniec, A.; Dorvaux, O.; Gall, B. J. P.; Roux, B.

    2008-07-15

    The medium-spin structure of the neutron-rich, odd-odd nucleus {sup 94}Rb was studied by means of {gamma}-ray spectroscopy. Excited levels were populated in the neutron-induced fission of {sup 235}U and in the spontaneous fission of {sup 252}Cf and {sup 248}Cm. Two isomeric states were found at 1485.2 and 2074.8 keV with half-lives of 18 and 107 ns, respectively. The probable structures of the two isomers involve the fully aligned, proton-neutron configurations [{pi}(g{sub 9/2}) x {nu}(g{sub 7/2})]{sub 8{sup +}} and [{pi}(g{sub 9/2}) x {nu}(h{sub 11/2})]{sub 10{sup -}}, respectively. These new data give information on the single-particle energies in the region.

  20. Magnetic resonance imaging of living systems by remote detection

    DOE Patents [OSTI]

    Wemmer, David; Pines, Alexander; Bouchard, Louis; Xu, Shoujun; Harel, Elad; Budker, Dmitry; Lowery, Thomas; Ledbetter, Micah

    2013-10-29

    A novel approach to magnetic resonance imaging is disclosed. Blood flowing through a living system is prepolarized, and then encoded. The polarization can be achieved using permanent or superconducting magnets. The polarization may be carried out upstream of the region to be encoded or at the place of encoding. In the case of an MRI of a brain, polarization of flowing blood can be effected by placing a magnet over a section of the body such as the heart upstream of the head. Alternatively, polarization and encoding can be effected at the same location. Detection occurs at a remote location, using a separate detection device such as an optical atomic magnetometer, or an inductive Faraday coil. The detector may be placed on the surface of the skin next to a blood vessel such as a jugular vein carrying blood away from the encoded region.

  1. Long-lived activation products in reactor materials

    SciTech Connect (OSTI)

    Evans, J.C.; Lepel, E.L.; Sanders, R.W.; Wilkerson, C.L.; Silker, W.; Thomas, C.W.; Abel, K.H.; Robertson, D.R.

    1984-08-01

    The purpose of this program was to assess the problems posed to reactor decommissioning by long-lived activation products in reactor construction materials. Samples of stainless steel, vessel steel, concrete, and concrete ingredients were analyzed for up to 52 elements in order to develop a data base of activatable major, minor, and trace elements. Large compositional variations were noted for some elements. Cobalt and niobium concentrations in stainless steel, for example, were found to vary by more than an order of magnitude. A thorough evaluation was made of all possible nuclear reactions that could lead to long lived activation products. It was concluded that all major activation products have been satisfactorily accounted for in decommissioning planning studies completed to date. A detailed series of calculations was carried out using average values of the measured compositions of the appropriate materials to predict the levels of activation products expected in reactor internals, vessel walls, and bioshield materials for PWR and BWR geometries. A comparison is made between calculated activation levels and regulatory guidelines for shallow land disposal according to 10 CFR 61. This analysis shows that PWR and BWR shroud material exceeds the Class C limits and is, therefore, generally unsuitable for near-surface disposal. The PWR core barrel material approaches the Class C limits. Most of the remaining massive components qualify as either Class A or B waste with the bioshield clearly Class A, even at the highest point of activation. Selected samples of activated steel and concrete were subjected to a limited radiochemical analysis program as a verification of the computer model. Reasonably good agreement with the calculations was obtained where comparison was possible. In particular, the presence of /sup 94/Nb in activated stainless steel at or somewhat above expected levels was confirmed.

  2. Air Leakage and Air Transfer Between Garage and Living Space

    SciTech Connect (OSTI)

    Rudd, A.

    2014-09-01

    This research project focused on evaluation of air transfer between the garage and living space in a single-family detached home constructed by a production homebuilder in compliance with the 2009 International Residential Code and the 2009 International Energy Conservation Code. The project gathered important information about the performance of whole-building ventilation systems and garage ventilation systems as they relate to minimizing flow of contaminated air from garage to living space. A series of 25 multi-point fan pressurization tests and additional zone pressure diagnostic testing characterized the garage and house air leakage, the garage-to-house air leakage, and garage and house pressure relationships to each other and to outdoors using automated fan pressurization and pressure monitoring techniques. While the relative characteristics of this house may not represent the entire population of new construction configurations and air tightness levels (house and garage) throughout the country, the technical approach was conservative and should reasonably extend the usefulness of the results to a large spectrum of house configurations from this set of parametric tests in this one house. Based on the results of this testing, the two-step garage-to-house air leakage test protocol described above is recommended where whole-house exhaust ventilation is employed. For houses employing whole-house supply ventilation (positive pressure) or balanced ventilation (same pressure effect as the Baseline condition), adherence to the EPA Indoor airPLUS house-to-garage air sealing requirements should be sufficient to expect little to no garage-to-house air transfer.

  3. Turning Bacteria into Fuel: Cyanobacteria Designed for Solar-Powered Highly Efficient Production of Biofuels

    SciTech Connect (OSTI)

    2010-01-01

    Broad Funding Opportunity Announcement Project: ASU is engineering a type of photosynthetic bacteria that efficiently produce fatty acidsa fuel precursor for biofuels. This type of bacteria, called Synechocystis, is already good at converting solar energy and carbon dioxide (CO2) into a type of fatty acid called lauric acid. ASU has modified the organism so it continuously converts sunlight and CO2 into fatty acidsoverriding its natural tendency to use solar energy solely for cell growth and maximizing the solar-to-fuel conversion process. ASUs approach is different because most biofuels research focuses on increasing cellular biomass and not on excreting fatty acids. The project has also identified a unique way to convert the harvested lauric acid into a fuel that can be easily blended with existing transportation fuels.

  4. Structure of the DUF2233 Domain in Bacteria and the Stuttering-associated

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    UCE Glycoprotein | Stanford Synchrotron Radiation Lightsource the DUF2233 Domain in Bacteria and the Stuttering-associated UCE Glycoprotein Wednesday, July 31, 2013 UCE figure DUF2233, a Domain of Unknown Function (DUF), is present in ~1200 bacterial and several viral and eukaryotic proteins. DUF2233 has been identified in proteins ranging in size from ~300-2000 residues. The 515 amino acid mammalian transmembrane glycoprotein α-N-acetylglucosamine-1-phosphodiester N-acetylglucosaminidase

  5. Bacteria Modified to Secrete Biologically Active Protein for Large-Scale

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Production - Energy Innovation Portal Bacteria Modified to Secrete Biologically Active Protein for Large-Scale Production Inventors: Sydnor Withers III, Miguel Dominguez, Matthew DeLisa, Charles Haitjema Great Lakes Bioenergy Research Center Contact GLBRC About This Technology Technology Marketing Summary E. coli is the most common prokaryote used to produce protein. The expressed protein generally accumulates in the cytoplasm. While this approach is useful for some proteins, not all

  6. Development of Microarrays-Based Metagenomics Technology for Monitoring Sulfate-Reducing Bacteria in Subsurface Environments

    SciTech Connect (OSTI)

    Cindy, Shi

    2015-07-17

    At the contaminated DOE sites, sulfate-reducing bacteria (SRB) are a significant population and play an important role in the microbial community during biostimulation for metal reduction. However, the diversity, structure and dynamics of SRB communities are poorly understood. Therefore, this project aims to use high throughput sequencing-based metagenomics technologies for characterizing the diversity, structure, functions, and activities of SRB communities by developing genomic and bioinformatics tools to link the SRB biodiversity with ecosystem functioning.

  7. New Metabolic Pathway Discovered in Methane-Consuming Bacteria | U.S. DOE

    Office of Science (SC) Website

    Office of Science (SC) New Metabolic Pathway Discovered in Methane-Consuming Bacteria Biological and Environmental Research (BER) BER Home About Research Facilities Science Highlights Searchable Archive of BER Highlights External link Benefits of BER Funding Opportunities Biological & Environmental Research Advisory Committee (BERAC) Community Resources Contact Information Biological and Environmental Research U.S. Department of Energy SC-23/Germantown Building 1000 Independence Ave., SW

  8. Cycling of DOC and DON by Novel Heterotrophic and Photoheterotrophic Bacteria in the Ocean: Final Report

    SciTech Connect (OSTI)

    Kirchman, David L

    2008-12-09

    The flux of dissolved organic matter (DOM) through aquatic bacterial communities is a major process in carbon cycling in the oceans and other aquatic systems. Our work addressed the general hypothesis that the phylogenetic make-up of bacterial communities and the abundances of key types of bacteria are important factors influencing the processing of DOM in aquatic ecosystems. Since most bacteria are not easily cultivated, the phylogenetic diversity of these microbes has to be assessed using culture-independent approaches. Even if the relevant bacteria were cultivated, their activity in the lab would likely differ from that under environmental conditions. This project found variation in DOM uptake by the major bacterial groups found in coastal waters. In brief, the data suggest substantial differences among groups in the use of high and molecular weight DOM components. It also made key discoveries about the role of light in affecting this uptake especially by cyanobacteria. In the North Atlantic Ocean, for example, over half of the light-stimulated uptake was by the coccoid cyanobacterium, Prochlorococcus, with the remaining uptake due to Synechococcus and other photoheterotrophic bacteria. The project also examined in detail the degradation of one organic matter component, chitin, which is often said to be the second most abundant compound in the biosphere. The findings of this project contribute to our understanding of DOM fluxes and microbial dynamics supported by those fluxes. It is possible that these findings will lead to improvements in models of the carbon cycle that have compartments for dissolved organic carbon (DOC), the largest pool of organic carbon in the oceans.

  9. Learning How Bacteria Communicate | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Learning How Bacteria Communicate Biological and Environmental Research (BER) BER Home About Research Facilities Science Highlights Searchable Archive of BER Highlights External link Benefits of BER Funding Opportunities Biological & Environmental Research Advisory Committee (BERAC) Community Resources Contact Information Biological and Environmental Research U.S. Department of Energy SC-23/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-3251 F: (301)

  10. A Scanning Auger Microprobe analysis of corrosion products associated with sulfate reducing bacteria

    SciTech Connect (OSTI)

    Sadowski, R.A.; Chen, G.; Clayton, C.R.; Kearns, J.R.; Gillow, J.B.; Francis, A.J.

    1995-03-01

    A Scanning Auger Microprobe analysis was performed on the corrosion products of an austenitic AISI type 304 SS after a potentiostatic polarization of one volt for ten minutes in a modified Postgate`s C media containing sulfate reducing bacteria. The corrosion products were characterized and mapped in local regions where pitting was observed. A critical evaluation of the applicability of this technique for the examination of microbially influenced corrosion (MIC) is presented.

  11. Environmental diagnostic analysis of ground water bacteria and their involvement in utilization of aromatic compounds

    SciTech Connect (OSTI)

    Wear, J.E. Jr.

    1993-05-01

    The objective of this study was to examine the hypothesis that select functional groups of bacteria from pristine sites have an innate ability to degrade synthetic aromatics that often contaminate groundwater environments,due to exposure to naturally occurring recalcitrant aromatics in their environment. This study demonstrates that subsurface microbial communities are capable of utilizing lignin and humic acid breakdown products. Utilizers of these compounds were found to be present in most all the wells tested. Even the deepest aquifer tested had utilizers present for all six of the aromatics tested. Highest counts for the aromatics tested were observed with the naturally occurring breakdown products of either lignin or humic acid. Carboxylic acids were found to be an important sole carbon source for groundwater bacteria possibly explained by the fact that they are produced by the oxidative cleavage of aromatic ring structures. The carbohydrate sole carbon sources that demonstrated the greatest densities were ones commonly associated with humics. This study indicates that utilization of naturally occurring aromatic compounds in the subsurface is an important nutritional source for groundwater bacteria. In addition, it suggests that adaptation to naturally occurring recalcitrant substrates is the origin of degradative pathways for xenobiotic compounds with analogous structure. This work has important implications for in situ bioremediation as a method of environmental cleanup.

  12. Isolation of butyrate-utilizing bacteria from thermophilic and mesophilic methane-producing ecosystems

    SciTech Connect (OSTI)

    Henson, J.M.

    1983-01-01

    The ability of various ecosystems to convert butyrate to methane was studied in order to isolate the bacteria responsible for the conversion. When thermophilic digester sludge was enriched with butyrate, methane was produced without a lag period. Marine sediments enriched with butyrate required a 2-week incubation period before methanogenesis began. A thermophilic digester was studied in more detail and found by most-probable-number enumeration to have ca. 5 x 10/sup 6/ butyrate-utilizing bactera/ml of sludge. A thermophilic butyrate-utilizing bacterium was isolated in coculture with Methanobacterium thermoautotrophicum and a Methanosarcina sp. This bacterium was a gram-negative, slightly curved rod that occurred singly, was nonmotile, and did not appear to produce spores. The thermophilic digester was infused with butyrate at the rate of 10 ..mu..moles/ml of sludge per day. Biogas production increased by 150%, with the percentage of methane increasing from 58% to 68%. Acetate, propionate, and butyrate did not accumulate. Butyrate-utilizing enrichments from mesophilic ecosystems were used in obtaining cocultures of butyrate-utilizing bacteria. These cocultures served as inocula for attempts to isolate pure cultures of butyrate-utilizing bacteria by use of hydrogenase-containing membrane fragments of Escherichia coli. After a 3-week incubation period, colonies appeared only in inoculated tubes that contained membrane fragments and butyrate.

  13. Reprogramming Bacteria to Seek and Destroy Small Molecules (JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)

    SciTech Connect (OSTI)

    Gallivan, Justin [Emory University] [Emory University

    2012-03-21

    Justin Gallivan, of Emory University presents a talk titled "Reprogramming Bacteria to Seek and Destroy Small Molecules" at the JGI User 7th Annual Genomics of Energy & Environment Meeting on March 21, 2012 in Walnut Creek, Calif

  14. Reprogramming Bacteria to Seek and Destroy Small Molecules (JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)

    ScienceCinema (OSTI)

    Gallivan, Justin [Emory University

    2013-01-22

    Justin Gallivan, of Emory University presents a talk titled "Reprogramming Bacteria to Seek and Destroy Small Molecules" at the JGI User 7th Annual Genomics of Energy & Environment Meeting on March 21, 2012 in Walnut Creek, Calif

  15. Liquid Fuel From Bacteria: Engineering Ralstonia eutropha for Production of Isobutanol (IBT) Motor Fuel from CO2, Hydrogen, and Oxygen

    SciTech Connect (OSTI)

    2010-07-15

    Electrofuels Project: MIT is using solar-derived hydrogen and common soil bacteria called Ralstonia eutropha to turn carbon dioxide (CO2) directly into biofuel. This bacteria already has the natural ability to use hydrogen and CO2 for growth. MIT is engineering the bacteria to use hydrogen to convert CO2 directly into liquid transportation fuels. Hydrogen is a flammable gas, so the MIT team is building an innovative reactor system that will safely house the bacteria and gas mixture during the fuel-creation process. The system will pump in precise mixtures of hydrogen, oxygen, and CO2, and the online fuel-recovery system will continuously capture and remove the biofuel product.

  16. Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Innovation and Deployment | Department of Energy Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and Deployment Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and Deployment October 19, 2011 - 3:00pm Addthis Washington, D.C. -- Thursday, October 20th, Richard Kauffman, Senior Adviser to the Secretary of Energy, will host the Department of Energy's third "Energy Matters" live chat. Kauffman will discuss the challenges and

  17. One Thing Leads to Another: How NETL Research Saves Lives | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy One Thing Leads to Another: How NETL Research Saves Lives One Thing Leads to Another: How NETL Research Saves Lives August 8, 2013 - 10:57am Addthis One Thing Leads to Another: How NETL Research Saves Lives Learn More The coronary stent was developed as part of NETL's Technology Transfer program. NETL's technology portfolio contains a broad range of innovations that have resulted from research in areas such as carbon capture and sequestration, mercury capture, fuel cells, sensors and

  18. Dr. Kathleen Hogan to Host Live Chat on Industrial Energy Efficiency |

    Office of Environmental Management (EM)

    Department of Energy Kathleen Hogan to Host Live Chat on Industrial Energy Efficiency Dr. Kathleen Hogan to Host Live Chat on Industrial Energy Efficiency November 16, 2011 - 9:40am Addthis WASHINGTON, D.C. - Kathleen Hogan, Assistant Deputy Secretary for Energy Efficiency, will host the Department of Energy's fourth "Energy Matters" live chat today. Dr. Hogan will discuss how the cost savings associated with industrial energy efficiency measures affect American companies and their

  19. Search for Long-Lived Particles ine+e-Collisions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lees, J. P.; Poireau, V.; Tisserand, V.; Grauges, E.; Palano, A.; Eigen, G.; Stugu, B.; Brown, D. N.; Kerth, L. T.; Kolomensky, Yu. G.; et al

    2015-04-29

    We present a search for a neutral, long-lived particle L that is produced in e+e- collisions and decays at a significant distance from the e+e- interaction point into various flavor combinations of two oppositely charged tracks. The analysis uses an e+e- data sample with a luminosity of 489.1 fb-1 collected by the BABAR detector at the γ (4S), γ (3S), and γ (2S) resonances and just below the γ (4S). Fitting the two-track mass distribution in search of a signal peak, we do not observe a significant signal, and set 90% confidence level upper limits on the product of themore »L production cross section, branching fraction, and reconstruction efficiency for six possible two-body L decay modes as a function of the L mass. The efficiency is given for each final state as a function of the mass, lifetime, and transverse momentum of the candidate, allowing application of the upper limits to any production model. In addition, upper limits are provided on the branching fraction B(B→XsL), where Xs is a strange hadronic system.« less

  20. Search for Long-Lived Particles ine+e-Collisions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lees, J. P.; Poireau, V.; Tisserand, V.; Grauges, E.; Palano, A.; Eigen, G.; Stugu, B.; Brown, D. N.; Kerth, L. T.; Kolomensky, Yu. G.; et al

    2015-04-29

    We present a search for a neutral, long-lived particle L that is produced in e+e- collisions and decays at a significant distance from the e+e- interaction point into various flavor combinations of two oppositely charged tracks. The analysis uses an e+e- data sample with a luminosity of 489.1 fb-1 collected by the BABAR detector at the γ (4S), γ (3S), and γ (2S) resonances and just below the γ (4S). Fitting the two-track mass distribution in search of a signal peak, we do not observe a significant signal, and set 90% confidence level upper limits on the product of themore » L production cross section, branching fraction, and reconstruction efficiency for six possible two-body L decay modes as a function of the L mass. The efficiency is given for each final state as a function of the mass, lifetime, and transverse momentum of the candidate, allowing application of the upper limits to any production model. In addition, upper limits are provided on the branching fraction B(B→XsL), where Xs is a strange hadronic system.« less

  1. Efficient Living in a Small House: Could You Make the Switch?

    Broader source: Energy.gov [DOE]

    The Small House Movement encourages homeowners to reject the huge homes that dominate many suburban neighborhoods and embrace smaller homes and simpler living.

  2. #AskEnergy: Join the Live Q&A on Wind Energy | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AskEnergy: Join the Live Q&A on Wind Energy #AskEnergy: Join the Live Q&A on Wind Energy August 18, 2014 - 8:52am Q&A Submit your wind energy questions for tomorrow's live Twitter Q&A now! Ask Us Addthis Have questions about wind energy? Experts from the Energy Department are answering your questions about wind during a live Twitter Q&A on Tuesday, August 19, at 1 p.m. ET. | Graphic by <a href="/node/379579">Sarah Gerrity</a>, Energy Department. Have

  3. Live Webinar on Better Buildings Case Competition: Energy Efficiency in the Restaurant Franchise Model Case Study

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webinar titled "A Side of Savings: Energy Efficiency in the Restaurant Franchise Model Case Study."

  4. Upcoming Live Discussion on Energy 101: Fuel Cells | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Live Discussion on Energy 101: Fuel Cells Upcoming Live Discussion on Energy 101: Fuel Cells January 10, 2014 - 12:00am Addthis Join the Energy Department at 2:00 p.m. ET on Thursday, January 16 for the first Energy 101 Google+ Hangout, which will focus on fuel cells.Submit questions for fuel cell experts in advance and during the hangout, and watch the discussion live on energy.gov/live or the Energy Department's Google+ page.Read more about this event and how to submit questions. Addthis

  5. 01-08-1998 - VPP Program Saves Lives | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    8-1998 - VPP Program Saves Lives Document Number: NA Effective Date: 01/1998 File (public): PDF icon 01-08-1998_green

  6. WPN 06-6: Weatherization Whole House Assessment Training Live Feed DVD

    Broader source: Energy.gov [DOE]

    To issue the Weatherization Whole-House Training Assessment Training Live Feed DVD to all grantees for use in the Weatherization Assistance Program (WAP).

  7. TODAY MAY 22ND: Live Tweeting the Apps for Energy Winners Announcement! |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy TODAY MAY 22ND: Live Tweeting the Apps for Energy Winners Announcement! TODAY MAY 22ND: Live Tweeting the Apps for Energy Winners Announcement! May 22, 2012 - 10:09am Addthis TODAY MAY 22ND: Live Tweeting the Apps for Energy Winners Announcement! Erin R. Pierce Erin R. Pierce Former Digital Communications Specialist, Office of Public Affairs How can I participate? Join us as we live tweet the Apps for Energy winners announcement event. To participate, simply follow

  8. Live Webinar on Better Buildings Case Competition: Taking Commercial PACE Financing to Scale, a Case Study

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webinar titled "A Side of Savings: Energy Efficiency in the Restaurant Franchise Model Case Study."

  9. MOLECULAR APPROACHES FOR IN SITU IDENTIFCIATION OF NITRATE UTILIZATION BY MARINE BACTERIA AND PHYTOPLANKTON

    SciTech Connect (OSTI)

    Frischer, Marc E.; Verity, Peter G.; Gilligan, Mathew R.; Bronk, Deborah A.; Zehr, Jonathan P.; Booth, Melissa G.

    2013-09-12

    Traditionally, the importance of inorganic nitrogen (N) for the nutrition and growth of marine phytoplankton has been recognized, while inorganic N utilization by bacteria has received less attention. Likewise, organic N has been thought to be important for heterotrophic organisms but not for phytoplankton. However, accumulating evidence suggests that bacteria compete with phytoplankton for nitrate (NO3-) and other N species. The consequences of this competition may have a profound effect on the flux of N, and therefore carbon (C), in ocean margins. Because it has been difficult to differentiate between N uptake by heterotrophic bacterioplankton versus autotrophic phytoplankton, the processes that control N utilization, and the consequences of these competitive interactions, have traditionally been difficult to study. Significant bacterial utilization of DIN may have a profound effect on the flux of N and C in the water column because sinks for dissolved N that do not incorporate inorganic C represent mechanisms that reduce the atmospheric CO2 drawdown via the ?biological pump? and limit the flux of POC from the euphotic zone. This project was active over the period of 1998-2007 with support from the DOE Biotechnology Investigations ? Ocean Margins Program (BI-OMP). Over this period we developed a tool kit of molecular methods (PCR, RT-PCR, Q-PCR, QRT-PCR, and TRFLP) and combined isotope mass spectrometry and flow-cytometric approaches that allow selective isolation, characterization, and study of the diversity and genetic expression (mRNA) of the structural gene responsible for the assimilation of NO3- by heterotrophic bacteria (nasA). As a result of these studies we discovered that bacteria capable of assimilating NO3- are ubiquitous in marine waters, that the nasA gene is expressed in these environments, that heterotrophic bacteria can account for a significant fraction of total DIN uptake in different ocean margin systems, that the expression of nasA is differentially regulated in genetically distinct NO3- assimilating bacteria, and that the best predictors of nasA gene expression are either NO3- concentration or NO3- uptake rates. These studies provide convincing evidence of the importance of bacterial utilization of NO3-, insight into controlling processes, and provide a rich dataset that are being used to develop linked C and N modeling components necessary to evaluate the significance of bacterial DIN utilization to global C cycling. Furthermore, as a result of BI-OMP funding we made exciting strides towards institutionalizing a research and education based collaboration between the Skidaway Institute of Oceanography (SkIO) and Savannah State University (SSU), an historically black university within the University System of Georgia with undergraduate and now graduate programs in marine science. The BI-OMP program, in addition to supporting undergraduate (24) graduate (10) and postdoctoral (2) students, contributed to the development of a new graduate program in Marine Sciences at SSU that remains an important legacy of this project. The long-term goals of these collaborations are to increase the capacity for marine biotechnology research and to increase representation of minorities in marine, environmental and biotechnological sciences.

  10. Exploration of Simple Analytical Approaches for Rapid Detection of Pathogenic Bacteria

    SciTech Connect (OSTI)

    Salma Rahman

    2005-12-17

    Many of the current methods for pathogenic bacterial detection require long sample-preparation and analysis time, as well as complex instrumentation. This dissertation explores simple analytical approaches (e.g., flow cytometry and diffuse reflectance spectroscopy) that may be applied towards ideal requirements of a microbial detection system, through method and instrumentation development, and by the creation and characterization of immunosensing platforms. This dissertation is organized into six sections. In the general Introduction section a literature review on several of the key aspects of this work is presented. First, different approaches for detection of pathogenic bacteria will be reviewed, with a comparison of the relative strengths and weaknesses of each approach, A general overview regarding diffuse reflectance spectroscopy is then presented. Next, the structure and function of self-assembled monolayers (SAMs) formed from organosulfur molecules at gold and micrometer and sub-micrometer patterning of biomolecules using SAMs will be discussed. This section is followed by four research chapters, presented as separate manuscripts. Chapter 1 describes the efforts and challenges towards the creation of imunosensing platforms that exploit the flexibility and structural stability of SAMs of thiols at gold. 1H, 1H, 2H, 2H-perfluorodecyl-1-thiol SAM (PFDT) and dithio-bis(succinimidyl propionate)-(DSP)-derived SAMs were used to construct the platform. Chapter 2 describes the characterization of the PFDT- and DSP-derived SAMs, and the architectures formed when it is coupled to antibodies as well as target bacteria. These studies used infrared reflection spectroscopy (IRS), X-ray photoelectron spectroscopy (XPS), and electrochemical quartz crystal microbalance (EQCM), Chapter 3 presents a new sensitive, and portable diffuse reflection based technique for the rapid identification and quantification of pathogenic bacteria. Chapter 4 reports research efforts in the construction and evaluation of a prototype flow cytometry based cell detector and enumerator. This final research chapter is followed by a general summation and future prospectus section that concludes this dissertation.

  11. Soft inertial microfluidics for high throughput separation of bacteria from human blood cells

    SciTech Connect (OSTI)

    Wu, Zhigang; Willing, Ben; Bjerketorp, Joakim; Jansson, Janet K.; Hjort, Klas

    2009-01-05

    We developed a new approach to separate bacteria from human blood cells based on soft inertial force induced migration with flow defined curved and focused sample flow inside a microfluidic device. This approach relies on a combination of an asymmetrical sheath flow and proper channel geometry to generate a soft inertial force on the sample fluid in the curved and focused sample flow segment to deflect larger particles away while the smaller ones are kept on or near the original flow streamline. The curved and focused sample flow and inertial effect were visualized and verified using a fluorescent dye primed in the device. First the particle behavior was studied in detail using 9.9 and 1.0 {micro}m particles with a polymer-based prototype. The prototype device is compact with an active size of 3 mm{sup 2}. The soft inertial effect and deflection distance were proportional to the fluid Reynolds number (Re) and particle Reynolds number (Re{sub p}), respectively. We successfully demonstrated separation of bacteria (Escherichia coli) from human red blood cells at high cell concentrations (above 10{sup 8}/mL), using a sample flow rate of up to 18 {micro}L/min. This resulted in at least a 300-fold enrichment of bacteria at a wide range of flow rates with a controlled flow spreading. The separated cells were proven to be viable. Proteins from fractions before and after cell separation were analyzed by gel electrophoresis and staining to verify the removal of red blood cell proteins from the bacterial cell fraction. This novel microfluidic process is robust, reproducible, simple to perform, and has a high throughput compared to other cell sorting systems. Microfluidic systems based on these principles could easily be manufactured for clinical laboratory and biomedical applications.

  12. Palladium nanoparticles produced by fermentatively cultivated bacteria as catalyst for diatrizoate removal with biogenic hydrogen

    SciTech Connect (OSTI)

    Hennebel, T.; Fitts, J.; Nevel, S. V.; Verschuere, S.; DeCorte, S.; DeGusseme, B.; Cuvelier, C.; vanderLelie, D.; Boon, N.; Verstraete, W.

    2011-05-17

    A new biological inspired method to produce nanopalladium is the precipitation of Pd on a bacterium, i.e., bio-Pd. This bio-Pd can be applied as catalyst in dehalogenation reactions. However, large amounts of hydrogen are required as electron donor in these reactions resulting in considerable costs. This study demonstrates how bacteria, cultivated under fermentative conditions, can be used to reductively precipitate bio-Pd catalysts and generate the electron donor hydrogen. In this way, one could avoid the costs coupled to hydrogen supply. The catalytic activities of Pd(0) nanoparticles produced by different strains of bacteria (bio-Pd) cultivated under fermentative conditions were compared in terms of their ability to dehalogenate the recalcitrant aqueous pollutants diatrizoate and trichloroethylene. While all of the fermentative bio-Pd preparations followed first order kinetics in the dehalogenation of diatrizoate, the catalytic activity differed systematically according to hydrogen production and starting Pd(II) concentration in solution. Batch reactors with nanoparticles formed by Citrobacter braakii showed the highest diatrizoate dehalogenation activity with first order constants of 0.45 {+-} 0.02 h{sup -1} and 5.58 {+-} 0.6 h{sup -1} in batches with initial concentrations of 10 and 50 mg L{sup -1} Pd, respectively. Nanoparticles on C. braakii, used in a membrane bioreactor treating influent containing 20 mg L{sup -1} diatrizoate, were capable of dehalogenating 22 mg diatrizoate mg{sup -1} Pd over a period of 19 days before bio-Pd catalytic activity was exhausted. This study demonstrates the possibility to use the combination of Pd(II), a carbon source and bacteria under fermentative conditions for the abatement of environmental halogenated contaminants.

  13. Lubricating bacteria model for the growth of bacterial colonies exposed to ultraviolet radiation

    SciTech Connect (OSTI)

    Zhang Shengli; Zhang Lei; Liang Run; Zhang Erhu; Liu Yachao; Zhao Shumin

    2005-11-01

    In this paper, we study the morphological transition of bacterial colonies exposed to ultraviolet radiation by modifying the bacteria model proposed by Delprato et al. Our model considers four factors: the lubricant fluid generated by bacterial colonies, a chemotaxis initiated by the ultraviolet radiation, the intensity of the ultraviolet radiation, and the bacteria's two-stage destruction rate with given radiation intensities. Using this modified model, we simulate the ringlike pattern formation of the bacterial colony exposed to uniform ultraviolet radiation. The following is shown. (1) Without the UV radiation the colony forms a disklike pattern and reaches a constant front velocity. (2) After the radiation is switched on, the bacterial population migrates to the edge of the colony and forms a ringlike pattern. As the intensity of the UV radiation is increased the ring forms faster and the outer velocity of the colony decreases. (3) For higher radiation intensities the total population decreases, while for lower intensities the total population increases initially at a small rate and then decreases. (4) After the UV radiation is switched off, the bacterial population grows both outward as well as into the inner region, and the colony's outer front velocity recovers to a constant value. All these results agree well with the experimental observations [Phys. Rev. Lett. 87, 158102 (2001)]. Along with the chemotaxis, we find that lubricant fluid and the two-stage destruction rate are critical to the dynamics of the growth of the bacterial colony when exposed to UV radiation, and these were not previously considered.

  14. Thiol-facilitated cell export and desorption of methylmercury by anaerobic bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lin, Hui; Lu, Xia; Liang, Liyuan; Gu, Baohua

    2015-09-04

    Neurotoxic methylmercury (MeHg), formed by anaerobic bacteria, is shown to be rapidly excreted from the cell, but the mechanism of this process is unclear. Using both Geobacter sulfurreducens PCA and Desulfovibrio desulfuricans ND132 strains, we investigated the factors affecting export and distribution of MeHg in mercury methylation and MeHg sorption-desorption assays. Thiols, such as cysteine, were found to greatly facilitate desorption and export of MeHg, particularly by PCA cells. However, in cysteine-free assays (4 h) >90% of the synthesized MeHg was associated with PCA, among which ~73% was sorbed on the cell surface and 19% remained inside the cells. Inmore » comparison, a majority of the MeHg (70%) was exported by ND132, leaving ~20% of the MeHg sorbed on the surface and 10% inside the cells. When MeHg was added directly to the cell suspensions, ND132 adsorbed much lower MeHg but took up more MeHg inside cells than PCA did. These results demonstrate that MeHg export is bacteria strain-specific, time dependent, and is influenced by thiols, implicating important roles of ligand complexation in facilitating MeHg production and mobilization in the environment.« less

  15. Size tunable elemental copper nanoparticles: extracellular synthesis by thermoanaerobic bacteria and capping molecules

    SciTech Connect (OSTI)

    Jang, Gyoung Gug; Jacobs, Christopher B; Gresback, Ryan G; Ivanov, Ilia N; Meyer III, Harry M; Kidder, Michelle; Joshi, Pooran C; Jellison Jr, Gerald Earle; Phelps, Tommy Joe; Graham, David E; Moon, Ji Won

    2015-01-01

    Bimodal sized elemental copper (Cu) nanoparticles (NPs) were synthesized from inexpensive oxidized copper salts by an extracellular metal-reduction process using anaerobic Thermoanaerobacter sp. X513 bacteria in aqueous solution. The bacteria nucleate NPs outside of the cell, and they control the Cu2+ reduction rate to form uniform crystallites with an average diameter of 1.75 0.46 m after 3-day incubation. To control the size and enhance air stability of Cu NPs, the reaction mixtures were supplemented with nitrilotriacetic acid as a chelator, and the surfactant capping agents oleic acid, oleylamine, ascorbic acid, or L-cysteine. Time-dependent UV-visible absorption measurements and XPS studies indicated well-suspended, bimodal colloidal Cu NPs (70 150 and 5 10 nm) with extended air-stability up to 300 min and stable Cu NP films surfaces with 14% oxidation after 20 days. FTIR spectroscopy suggested that these capping agents were effectively adsorbed on the NP surface providing oxidation resistance in aqueous and dry conditions. Compared to previously reported Cu NP syntheses, this biological process substantially reduced the requirement for hazardous organic solvents and chemical reducing agents, while reducing the levels of Cu oxide impurities in the product. This process was highly reproducible and scalable from 0.01 to 1-L batches.

  16. Discovery of novel plant interaction determinants from the genomes of 163 root nodule bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Seshadri, Rekha; Reeve, Wayne G.; Ardley, Julie K.; Tennessen, Kristin; Woyke, Tanja; Kyrpides, Nikos C.; Ivanova, Natalia N.

    2015-11-20

    Root nodule bacteria (RNB) or “rhizobia” are a type of plant growth promoting bacteria, typified by their ability to fix nitrogen for their plant host, fixing nearly 65% of the nitrogen currently utilized in sustainable agricultural production of legume crops and pastures. In this study, we sequenced the genomes of 110 RNB from diverse hosts and biogeographical regions, and undertook a global exploration of all available RNB genera with the aim of identifying novel genetic determinants of symbiotic association and plant growth promotion. Specifically, we performed a subtractive comparative analysis with non-RNB genomes, employed relevant transcriptomic data, and leveraged phylogeneticmore » distribution patterns and sequence signatures based on known precepts of symbioticand host-microbe interactions. A total of 184 protein families were delineated, including known factors for nodulation and nitrogen fixation, and candidates with previously unexplored functions, for which a role in host-interaction, -regulation, biocontrol, and more, could be posited. Lastly, these analyses expand our knowledge of the RNB purview and provide novel targets for strain improvement in the ultimate quest to enhance plant productivity and agricultural sustainability.« less

  17. Thiol-facilitated cell export and desorption of methylmercury by anaerobic bacteria

    SciTech Connect (OSTI)

    Lin, Hui; Lu, Xia; Liang, Liyuan; Gu, Baohua

    2015-09-04

    Neurotoxic methylmercury (MeHg), formed by anaerobic bacteria, is shown to be rapidly excreted from the cell, but the mechanism of this process is unclear. Using both Geobacter sulfurreducens PCA and Desulfovibrio desulfuricans ND132 strains, we investigated the factors affecting export and distribution of MeHg in mercury methylation and MeHg sorption-desorption assays. Thiols, such as cysteine, were found to greatly facilitate desorption and export of MeHg, particularly by PCA cells. However, in cysteine-free assays (4 h) >90% of the synthesized MeHg was associated with PCA, among which ~73% was sorbed on the cell surface and 19% remained inside the cells. In comparison, a majority of the MeHg (70%) was exported by ND132, leaving ~20% of the MeHg sorbed on the surface and 10% inside the cells. When MeHg was added directly to the cell suspensions, ND132 adsorbed much lower MeHg but took up more MeHg inside cells than PCA did. These results demonstrate that MeHg export is bacteria strain-specific, time dependent, and is influenced by thiols, implicating important roles of ligand complexation in facilitating MeHg production and mobilization in the environment.

  18. Turning Bacteria into Biofuel: Development of an Integrated Microbial Electrocatalytic (MEC) System for Liquid Biofuel Production from CO2

    SciTech Connect (OSTI)

    2010-08-01

    Electrofuels Project: LBNL is improving the natural ability of a common soil bacteria called Ralstonia eutropha to use hydrogen and carbon dioxide for biofuel production. First, LBNL is genetically modifying the bacteria to produce biofuel at higher concentrations. Then, LBNL is using renewable electricity obtained from solar, wind, or wave power to produce high amounts of hydrogen in the presence of the bacteria—increasing the organism’s access to its energy source and improving the efficiency of the biofuel-creation process. Finally, LBNL is tethering electrocatalysts to the bacteria’s surface which will further accelerate the rate at which the organism creates biofuel. LBNL is also developing a chemical method to transform the biofuel that the bacteria produce into ready-to-use jet fuel.

  19. Anyone can learn about plasma physics through live-streamed course at PPPL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | Princeton Plasma Physics Lab Anyone can learn about plasma physics through live-streamed course at PPPL By Jeanne Jackson DeVoe June 5, 2015 Tweet Widget Google Plus One Share on Facebook A screenshot of the new SULI website where a course in plasma physics will be live-streamed. A screenshot of the new SULI website where a course in plasma physics will be live-streamed. If you've always wanted to learn about the science behind plasma physics and fusion energy, you can listen to the very

  20. Anyone can learn about plasma physics through live-streamed course at PPPL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | Princeton Plasma Physics Lab Anyone can learn about plasma physics through live-streamed course at PPPL By Jeanne Jackson DeVoe June 5, 2015 Tweet Widget Google Plus One Share on Facebook A screenshot of the new SULI website where a course in plasma physics will be live-streamed. A screenshot of the new SULI website where a course in plasma physics will be live-streamed. If you've always wanted to learn about the science behind plasma physics and fusion energy, you can listen to the very

  1. TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    America's Oil Dependence Through Innovation | Department of Energy ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's Oil Dependence Through Innovation TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's Oil Dependence Through Innovation June 28, 2011 - 12:00am Addthis WASHINGTON, D.C. - Today, Wednesday, June 29, ARPA-E Director Arun Majumdar will host the Department of Energy's second "Energy Matters" live chat. Dr. Majumdar will

  2. TOMORROW: Secretary Chu To Host Earth Day Live Chat | Department of Energy

    Energy Savers [EERE]

    Secretary Chu To Host Earth Day Live Chat TOMORROW: Secretary Chu To Host Earth Day Live Chat April 19, 2012 - 12:10pm Addthis WASHINGTON, DC - On Friday, April 20, 2012, U.S. Energy Secretary Steven Chu will host a livechat as part of the Energy Department's Earth Day celebrations to highlight the environmental and economic benefits of the Department's investments in clean energy. The live chat will be streamed on www.energy.gov tomorrow, and will feature questions from both in-person and

  3. Our Energy Independence - A Live Chat With Dr. Arun Majumdar | Department

    Energy Savers [EERE]

    of Energy Energy Independence - A Live Chat With Dr. Arun Majumdar Our Energy Independence - A Live Chat With Dr. Arun Majumdar June 29, 2011 - 11:16am Addthis Dr. Arun Majumdar takes your questions about investments we're making to build the clean energy infrastructure of the future. Ginny Simmons Ginny Simmons Former Managing Editor for Energy.gov, Office of Public Affairs How can I participate? Submit a question via E-mail, Facebook or Twitter Watch live at 2 PM [UPDATED with video from

  4. Night of the Living Trash: Bringing Your Waste Back to Life | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Night of the Living Trash: Bringing Your Waste Back to Life Night of the Living Trash: Bringing Your Waste Back to Life October 30, 2015 - 12:33pm Addthis Night of the Living Trash: Bringing Your Waste Back to Life Dr. Valerie Sarisky-Reed Dr. Valerie Sarisky-Reed Deputy Director, Bioenergy Technologies Office This Halloween season, the U.S. Department of Energy's Bioenergy Technologies Office (BETO) is highlighting how waste can be "brought back to life" and turned into

  5. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Breakthrough antibacterial approach could resolve serious skin infections August 26, 2014 Los Alamos and partners test ionic liquids to break bacterial biofilm layer and save lives LOS ALAMOS, N.M., Aug. 26, 2014-Like a protective tent over a colony of harmful bacteria, biofilms make the treatment of skin infections especially difficult. Microorganisms protected in a biofilm pose a significant health risk due to their antibiotic resistance and recalcitrance to treatment, and biofilm-protected

  6. Environmental Microbiology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Environmental Microbiology Examining the soil beneath our feet Read caption + Many environmental molecular biology studies begin with purified DNA and RNA extracted from the soil. Overview of Research and Highlights Learning about microorganisms-bacteria, algae, and fungi-is essential to understanding how living things interact with their environments. Exploration of environmental microbiology at Los Alamos crosses broad scales of investigation that span from identification of genetic regulatory

  7. Structure, Function, and Regulation of Antenna Complexes of Green Photosynthetic Bacteria

    SciTech Connect (OSTI)

    Robert E. Blankenship

    2001-04-27

    This project is concerned with the structure and function of the chlorosome antennas found in green photosynthetic bacteria. Chlorosomes are ellipsoidal structures attached to the cytoplasmic side of the inner cell membrane. These antenna complexes provide a very large absorption cross section for light capture. Evidence is overwhelming that the chlorosome represents a very different type of antenna from that found in any other photosynthetic system yet studied. It is now clear that chlorosomes do not contain traditional pigment-proteins, in which the pigments bind to specific sites on proteins. Instead, the chlorosome pigments are organized in vivo into pigment oligomers in which direct pigment-pigment interactions are of dominant importance. Our group has used a multidisciplinary approach to investigate this unique system, including model systems, ultrafast spectroscopy, molecular biology, protein chemistry and X-ray crystallography.

  8. Live Webinar on the Funding Opportunity for Technology Incubator for Wind Energy Innovations

    Broader source: Energy.gov [DOE]

    On April 17, 2014, from 1:00 – 3:00 PM MDT, the Wind Program will hold a live webinar to provide information to potential applicants for the Technology Incubator for Wind Energy Innovations Funding Opportunity.

  9. Live Webcast on the 2014 Farm Bill's Renewable Energy for America Program

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webcast titled “The 2014 Farm Bill's Renewable Energy for America Program” on May 21, 2014, from 3:00 to 4:00 p.m. Eastern Standard Time.

  10. Live Webinar on the Funding Opportunity for Marine and Hydrokinetic Research and Development University Consortium

    Broader source: Energy.gov [DOE]

    On April 24, 2014 from 1:00 - 2:30 PM EDT, the Water Power Program will hold a live webinar to provide information to potential applicants for the Marine and Hydrokinetic (MHK) Research and...

  11. Search for long-lived particles that decay into final states...

    Office of Scientific and Technical Information (OSTI)

    obtained are the most stringent to date. In the specific case of a model in which a Higgs boson in the mass range 125-1000mathrmGeVc2 decays into a pair of long-lived...

  12. Anyone can learn about plasma physics through live-streamed course...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Anyone can learn about plasma physics through live-streamed course at PPPL By Jeanne Jackson DeVoe June 5, 2015 Tweet Widget Google Plus One Share on Facebook A screenshot of the...

  13. Live Webinar on the Funding Opportunity for Wind Forecasting Improvement Project in Complex Terrain

    Broader source: Energy.gov [DOE]

    On April 21, 2014 from 3:00 to 5:00 PM EST the Wind Program will hold a live webinar to provide information to potential applicants for this Funding Opportunity Announcement. There is no cost to...

  14. WATCH LIVE: Secretary Chu at 10:45 am ET | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    WATCH LIVE: Secretary Chu at 10:45 am ET April 20, 2012 - 9:00am Addthis Amanda Scott Amanda Scott Former Managing Editor, Energy.gov This morning, at 10:45 am ET, we'll be ...

  15. Long-lived nanosecond spin relaxation and spin coherence of electrons...

    Office of Scientific and Technical Information (OSTI)

    Long-lived nanosecond spin relaxation and spin coherence of electrons in monolayer MoS2 and WS2 Citation Details In-Document Search This content will become publicly available on ...

  16. The secret lives of scientists & engineers: Bill Gasper | Argonne National

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laboratory This article was published in the spring 2014 issue of Argonne Now, the laboratory science magazine. The secret lives of scientists & engineers: Bill Gasper June 1, 2014 Tweet EmailPrint This story was originally published in volume 8, issue 1 of Argonne Now, the laboratory's semiannual science magazine. Meet researchers from Argonne with unusual hobbies and interests in this series from the lab magazine, The Secret Lives of Scientists & Engineers. Today we're interviewing

  17. Global and regional evolution of short-lived radiatively-active gases and

    Office of Scientific and Technical Information (OSTI)

    aerosols in the Representative Concentration Pathways (Journal Article) | SciTech Connect Global and regional evolution of short-lived radiatively-active gases and aerosols in the Representative Concentration Pathways Citation Details In-Document Search Title: Global and regional evolution of short-lived radiatively-active gases and aerosols in the Representative Concentration Pathways In this paper, we discuss the results of 2000-2100 simulations with a chemistry-climate model, focusing on

  18. University Teams to Showcase Affordable, Energy Efficient Living in U.S.

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Solar Decathlon 2011 | Department of Energy to Showcase Affordable, Energy Efficient Living in U.S. Department of Energy Solar Decathlon 2011 University Teams to Showcase Affordable, Energy Efficient Living in U.S. Department of Energy Solar Decathlon 2011 September 22, 2011 - 10:32am Addthis WASHINGTON, DC - Collegiate teams featuring over 4,000 students from around the world have descended on the National Mall's West Potomac Park to showcase the highly energy efficient

  19. Deformation Quantization: Quantum Mechanic Lives and Works in Phase-Space

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (Conference) | SciTech Connect Conference: Deformation Quantization: Quantum Mechanic Lives and Works in Phase-Space Citation Details In-Document Search Title: Deformation Quantization: Quantum Mechanic Lives and Works in Phase-Space Wigner's 1932 quasi-probability Distribution Function in phase-space is a special (Weyl) representation of the density matrix. It has been useful in describing quantum flows in: quantum optics; nuclear physics; decoherence (eg, quantum computing); quantum chaos;

  20. Join Us Live This Friday: Women in Clean Energy Symposium | Department of

    Office of Environmental Management (EM)

    Energy Live This Friday: Women in Clean Energy Symposium Join Us Live This Friday: Women in Clean Energy Symposium September 26, 2012 - 2:19pm Addthis NREL researcher Kirsten Alberi works in the luminescence mapping laboratory at the Solar Energy Research Facility (SERF). Here she aligns a laser and sample to map the photoluminescence coming off the sample. | Photo by Dennis Schroeder, NREL. NREL researcher Kirsten Alberi works in the luminescence mapping laboratory at the Solar Energy

  1. Detecting bacteria and Determining Their Susceptibility to Antibiotics by Stochastic Confinement in Nanoliter Droplets using Plug-Based Microfluidics

    SciTech Connect (OSTI)

    Boedicker, J.; Li, L; Kline, T; Ismagilov, R

    2008-01-01

    This article describes plug-based microfluidic technology that enables rapid detection and drug susceptibility screening of bacteria in samples, including complex biological matrices, without pre-incubation. Unlike conventional bacterial culture and detection methods, which rely on incubation of a sample to increase the concentration of bacteria to detectable levels, this method confines individual bacteria into droplets nanoliters in volume. When single cells are confined into plugs of small volume such that the loading is less than one bacterium per plug, the detection time is proportional to plug volume. Confinement increases cell density and allows released molecules to accumulate around the cell, eliminating the pre-incubation step and reducing the time required to detect the bacteria. We refer to this approach as stochastic confinement. Using the microfluidic hybrid method, this technology was used to determine the antibiogram - or chart of antibiotic sensitivity - of methicillin-resistant Staphylococcus aureus (MRSA) to many antibiotics in a single experiment and to measure the minimal inhibitory concentration (MIC) of the drug cefoxitin (CFX) against this strain. In addition, this technology was used to distinguish between sensitive and resistant strains of S. aureus in samples of human blood plasma. High-throughput microfluidic techniques combined with single-cell measurements also enable multiple tests to be performed simultaneously on a single sample containing bacteria. This technology may provide a method of rapid and effective patient-specific treatment of bacterial infections and could be extended to a variety of applications that require multiple functional tests of bacterial samples on reduced timescales.

  2. Impact of bacteria and bacterial components on osteogenic and adipogenic differentiation of adipose-derived mesenchymal stem cells

    SciTech Connect (OSTI)

    Fiedler, Tomas; Salamon, Achim; Adam, Stefanie; Herzmann, Nicole; Taubenheim, Jan; Peters, Kirsten

    2013-11-01

    Adult mesenchymal stem cells (MSC) are present in several tissues, e.g. bone marrow, heart muscle, brain and subcutaneous adipose tissue. In invasive infections MSC get in contact with bacteria and bacterial components. Not much is known about how bacterial pathogens interact with MSC and how contact to bacteria influences MSC viability and differentiation potential. In this study we investigated the impact of three different wound infection relevant bacteria, Escherichia coli, Staphylococcus aureus, and Streptococcus pyogenes, and the cell wall components lipopolysaccharide (LPS; Gram-negative bacteria) and lipoteichoic acid (LTA; Gram-positive bacteria) on viability, proliferation, and osteogenic as well as adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells (adMSC). We show that all three tested species were able to attach to and internalize into adMSC. The heat-inactivated Gram-negative E. coli as well as LPS were able to induce proliferation and osteogenic differentiation but reduce adipogenic differentiation of adMSC. Conspicuously, the heat-inactivated Gram-positive species showed the same effects on proliferation and adipogenic differentiation, while its cell wall component LTA exhibited no significant impact on adMSC. Therefore, our data demonstrate that osteogenic and adipogenic differentiation of adMSC is influenced in an oppositional fashion by bacterial antigens and that MSC-governed regeneration is not necessarily reduced under infectious conditions. - Highlights: Staphylococcus aureus, Streptococcus pyogenes and Escherichia coli bind to and internalize into adMSC. Heat-inactivated cells of these bacterial species trigger proliferation of adMSC. Heat-inactivated E. coli and LPS induce osteogenic differentiation of adMSC. Heat-inactivated E. coli and LPS reduce adipogenic differentiation of adMSC. LTA does not influence adipogenic or osteogenic differentiation of adMSC.

  3. We Want to Hear From You: Live Twitter Q&A Tuesday at Noon | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Want to Hear From You: Live Twitter Q&A Tuesday at Noon We Want to Hear From You: Live Twitter Q&A Tuesday at Noon March 19, 2012 - 11:07am Addthis Visit twitter.com/energy to join our live Twitter Q&A on Tuesday, March 20, at noon ET. Visit twitter.com/energy to join our live Twitter Q&A on Tuesday, March 20, at noon ET. Erin R. Pierce Erin R. Pierce Former Digital Communications Specialist, Office of Public Affairs How can I participate? To join our live Q&A,

  4. Bioenergetic studies of coal sulfur oxidation by extremely thermophilic bacteria. Final report, September 15, 1992--August 31, 1997

    SciTech Connect (OSTI)

    Kelly, R.M.; Han, C.J.

    1997-12-31

    Thermoacidophilic microorganisms have been considered for inorganic sulfur removal from coal because of expected improvements in rates of both biotic and abiotic sulfur oxidation reactions with increasing temperature. In this study, the bioenergetic response of the extremely thermoacidophilic archaeon, Metallosphaera sedula, to environmental changes have been examined in relation to its capacity to catalyze pyrite oxidation in coal. Given an appropriate bioenergetic challenge, the metabolic response was to utilize additional amounts of energy sources (i.e., pyrite) to survive. Of particular interest were the consequences of exposing the organism to various forms of stress (chemical, nutritional, thermal, pH) in the presence of coal pyrite. Several approaches to take advantage of stress response to accelerate pyrite oxidation by this organism were examined, including attempts to promote acquired thermal tolerance to extend its functional range, exposure to chemical uncouplers and decouplers, and manipulation of heterotrophic and chemolithotrophic tendencies to optimize biomass concentration and biocatalytic activity. Promising strategies were investigated in a continuous culture system. This study identified environmental conditions that promote better coupling of biotic and abiotic oxidation reactions to improve biosulfurization rates of thermoacidophilic microorganisms.

  5. Structural characterization and comparison of three acyl-carrier-protein synthases from pathogenic bacteria

    SciTech Connect (OSTI)

    Halavaty, Andrei S. [Center for Structural Genomics of Infectious Diseases, (United States); Northwestern University, Chicago, IL 60611 (United States); Kim, Youngchang [Center for Structural Genomics of Infectious Diseases, (United States); Argonne National Laboratory, Argonne, IL 60439 (United States); University of Chicago, Chicago, IL 60637 (United States); Minasov, George; Shuvalova, Ludmilla; Dubrovska, Ievgeniia; Winsor, James [Center for Structural Genomics of Infectious Diseases, (United States); Northwestern University, Chicago, IL 60611 (United States); Zhou, Min [Center for Structural Genomics of Infectious Diseases, (United States); Argonne National Laboratory, Argonne, IL 60439 (United States); University of Chicago, Chicago, IL 60637 (United States); Onopriyenko, Olena; Skarina, Tatiana [Center for Structural Genomics of Infectious Diseases, (United States); University of Toronto, Toronto, Ontario M5G 1L6 (Canada); Papazisi, Leka; Kwon, Keehwan; Peterson, Scott N. [Center for Structural Genomics of Infectious Diseases, (United States); J. Craig Venter Institute, Rockville, MD 20850 (United States); Joachimiak, Andrzej [Center for Structural Genomics of Infectious Diseases, (United States); Argonne National Laboratory, Argonne, IL 60439 (United States); University of Chicago, Chicago, IL 60637 (United States); Savchenko, Alexei [Center for Structural Genomics of Infectious Diseases, (United States); University of Toronto, Toronto, Ontario M5G 1L6 (Canada); Anderson, Wayne F., E-mail: wf-anderson@northwestern.edu [Center for Structural Genomics of Infectious Diseases, (United States); Northwestern University, Chicago, IL 60611 (United States)

    2012-10-01

    The structural characterization of acyl-carrier-protein synthase (AcpS) from three different pathogenic microorganisms is reported. One interesting finding of the present work is a crystal artifact related to the activity of the enzyme, which fortuitously represents an opportunity for a strategy to design a potential inhibitor of a pathogenic AcpS. Some bacterial type II fatty-acid synthesis (FAS II) enzymes have been shown to be important candidates for drug discovery. The scientific and medical quest for new FAS II protein targets continues to stimulate research in this field. One of the possible additional candidates is the acyl-carrier-protein synthase (AcpS) enzyme. Its holo form post-translationally modifies the apo form of an acyl carrier protein (ACP), which assures the constant delivery of thioester intermediates to the discrete enzymes of FAS II. At the Center for Structural Genomics of Infectious Diseases (CSGID), AcpSs from Staphylococcus aureus (AcpS{sub SA}), Vibrio cholerae (AcpS{sub VC}) and Bacillus anthracis (AcpS{sub BA}) have been structurally characterized in their apo, holo and product-bound forms, respectively. The structure of AcpS{sub BA} is emphasized because of the two 3?, 5?-adenosine diphosphate (3?, 5?-ADP) product molecules that are found in each of the three coenzyme A (CoA) binding sites of the trimeric protein. One 3?, 5?-ADP is bound as the 3?, 5?-ADP part of CoA in the known structures of the CoAAcpS and 3?, 5?-ADPAcpS binary complexes. The position of the second 3?, 5?-ADP has never been described before. It is in close proximity to the first 3?, 5?-ADP and the ACP-binding site. The coordination of two ADPs in AcpS{sub BA} may possibly be exploited for the design of AcpS inhibitors that can block binding of both CoA and ACP.

  6. South Atlantic OCS area living marine resources study. Volume II: an investigation of live bottom habitats north of Cape Fear, North Carolina. Final report

    SciTech Connect (OSTI)

    Not Available

    1981-10-01

    The major objectives of this study were to (1) characterize benthic and nektonic communities associated with representative live bottom habitats on the continental shelf of the South Atlantic Bight, and (2) evaluate factors which might influence these communities, particularly the potential for impact by offshore oil and gas activities. The report describes three study sites at the edge of the continental shelf in a 55-100m depth zone, near Cape Fear, North Carolina.

  7. Alteration of Iron-Rich Lacustrine Sediments by Dissimilatory Iron-Reducing Bacteria

    SciTech Connect (OSTI)

    Crowe,S.; Roberts, J.; Weisener, C.; Fowle, D.

    2007-01-01

    The reduction of Fe during bacterial anaerobic respiration in sediments and soils not only causes the degradation of organic matter but also results in changes in mineralogy and the redistribution of many nutrients and trace metals. Understanding trace metal patterns in sedimentary rocks and predicting the fate of contaminants in the environment requires a detailed understanding of the mechanisms through which they are redistributed during Fe reduction. In this work, lacustrine sediments from Lake Matano in Indonesia were incubated in a minimal media with the dissimilatory iron reducing (DIR) bacterium Shewanella putrefaciens 200R. These sediments were reductively dissolved at rates slower than pure synthetic goethite despite the presence of an 'easily reducible' component, as defined by selective extractions. DIR of the lacustrine sediments resulted in the substrate-dependent production of abundant quantities of extracellular polymeric substances. Trace elements, including Ni, Co, P, Si, and As, were released from the sediments with progressive Fe reduction while Cr was sequestered. Much of the initial trace metal mobility can be attributed to the rapid reduction of a Mn-rich oxyhydroxide phase. The production of organo-Fe(III) reveals that DIR bacteria can generate significant metal complexation capacity. This work demonstrates that DIR induces the release of many elements associated with Fe-Mn oxyhydroxides, despite secondary mineralization.

  8. Genotypic and phenotypic characterization of aerosolized bacteria collected from African dust events

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wilson, Christina A.; Brigmon, Robin L.; Yeager, Chris; Smith, Garriet W.; Polson, Shawn W.

    2013-07-31

    Twenty-one bacteria were isolated and characterized from air samples collected in Africa and the Caribbean by the United States Geological Survey (USGS). Isolates were selected based on preliminary characterization as possible pathogens. Identification of the bacterial isolates was 25 achieved using 16S rRNA gene sequence analysis, fatty acid methyl esters (FAMEs) profiling, the BIOLOG Microlog® System (carbon substrate assay), and repetitive extragenic palindromic (REP)-PCR analysis. The majority of isolates (18/21) were identified as species of the genus Bacillus. Three isolates were classified within the Bacillus cereus senso lato group, which includes Bacillus anthracis, Bacillus thuringiensis, and Bacillus cereus strains. Onemore » isolate was identified as a Staphylococcus sp., 30 most closely related to species (i.e Staphylococcus kloosii, Staphylococcus warneri) that are commonly associated with human or animal skin, but can also act as opportunistic pathogen. Another isolate was tentatively identified as Tsukamurella inchonensis, a known respiratory pathogen, and was resistant to the ten antibiotics tested including vancomycin.« less

  9. Genotypic and phenotypic characterization of aerosolized bacteria collected from African dust events

    SciTech Connect (OSTI)

    Wilson, Christina A.; Brigmon, Robin L.; Yeager, Chris; Smith, Garriet W.; Polson, Shawn W.

    2013-07-31

    Twenty-one bacteria were isolated and characterized from air samples collected in Africa and the Caribbean by the United States Geological Survey (USGS). Isolates were selected based on preliminary characterization as possible pathogens. Identification of the bacterial isolates was 25 achieved using 16S rRNA gene sequence analysis, fatty acid methyl esters (FAMEs) profiling, the BIOLOG Microlog System (carbon substrate assay), and repetitive extragenic palindromic (REP)-PCR analysis. The majority of isolates (18/21) were identified as species of the genus Bacillus. Three isolates were classified within the Bacillus cereus senso lato group, which includes Bacillus anthracis, Bacillus thuringiensis, and Bacillus cereus strains. One isolate was identified as a Staphylococcus sp., 30 most closely related to species (i.e Staphylococcus kloosii, Staphylococcus warneri) that are commonly associated with human or animal skin, but can also act as opportunistic pathogen. Another isolate was tentatively identified as Tsukamurella inchonensis, a known respiratory pathogen, and was resistant to the ten antibiotics tested including vancomycin.

  10. "Lantern Live" Mobile App Lights Way for Citizens Impacted by Disasters |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy "Lantern Live" Mobile App Lights Way for Citizens Impacted by Disasters "Lantern Live" Mobile App Lights Way for Citizens Impacted by Disasters November 24, 2014 - 5:06pm Addthis (Editor's Note: the following was originally posted on the White House Office of Science and Technology Policy blog on November 21, 2014.) By Brian Forde, Denice Ross and Derek Frempong In the wake of Hurricane Sandy, seemingly simple tasks such as refueling your car were

  11. Long-lived nanosecond spin relaxation and spin coherence of electrons in

    Office of Scientific and Technical Information (OSTI)

    monolayer MoS2 and WS2 (Journal Article) | SciTech Connect Long-lived nanosecond spin relaxation and spin coherence of electrons in monolayer MoS2 and WS2 Citation Details In-Document Search This content will become publicly available on August 3, 2016 Title: Long-lived nanosecond spin relaxation and spin coherence of electrons in monolayer MoS2 and WS2 The recently discovered monolayer transition metal dichalcogenides (TMDCs) provide a fertile playground to explore new coupled spin-valley

  12. Table HC1.2.2 Living Space Characteristics by Average Floorspace

    U.S. Energy Information Administration (EIA) Indexed Site

    2 Living Space Characteristics by Average Floorspace, " " Per Housing Unit and Per Household Member, 2005" ,,"Average Square Feet" ," Housing Units (millions)" ,,"Per Housing Unit",,,"Per Household Member" "Living Space Characteristics",,"Total1","Heated","Cooled","Total1","Heated","Cooled" "Total",111.1,2033,1618,1031,791,630,401 "Total Floorspace (Square

  13. Table HC1.2.4 Living Space Characteristics by Average Floorspace--Apartments, 2

    U.S. Energy Information Administration (EIA) Indexed Site

    2.4 Living Space Characteristics by Average Floorspace--Apartments, 2005" ,,,"Average Square Feet per Apartment in a --" ," Housing Units (millions)" ,,,"2 to 4 Unit Building",,,"5 or More Unit Building" ,,"Apartments (millions)" "Living Space Characteristics",,,"Total","Heated","Cooled","Total","Heated","Cooled" "Total",111.1,24.5,1090,902,341,872,780,441

  14. DOE Tour of Zero: Options for Community Living by United Way of Long Island

    Energy Savers [EERE]

    | Department of Energy Options for Community Living by United Way of Long Island DOE Tour of Zero: Options for Community Living by United Way of Long Island 1 of 14 United Way of Long Island Housing Development Corporation renovated this 1,436-square-foot home in Patchogue, New York, to the performance criteria of the U.S. Department of Energy Zero Energy Ready Home (ZERH) program. 2 of 14 The 1970s-era home had a HERS score of 162 in its pre-renovation condition, as shown here. When

  15. Follow Live Dec 24: Los Alamos National Lab Tracks Rudolph's Nose,

    Broader source: Energy.gov (indexed) [DOE]

    Santa's Sleigh | Department of Energy Thanks to the Los Alamos National Laboratory, you can <a href="http://www.santa.lanl.gov/"> follow along live</a> as Santa circles the globe this Christmas Eve. | Image credit: Hantz Leger. Thanks to the Los Alamos National Laboratory, you can follow along live as Santa circles the globe this Christmas Eve. | Image credit: Hantz Leger. Ginny Simmons Ginny Simmons Former Managing Editor for Energy.gov, Office of Public Affairs What

  16. "Table HC11.2 Living Space Characteristics by Northeast Census Region, 2005"

    U.S. Energy Information Administration (EIA) Indexed Site

    2 Living Space Characteristics by Northeast Census Region, 2005" " Million U.S. Housing Units" ,,"Northeast Census Region" ,"U.S. Housing Units (millions)" ,,,"Census Division" ,,"Total Northeast" "Living Space Characteristics",,,"Middle Atlantic","New England" "Total",111.1,20.6,15.1,5.5 "Floorspace (Square Feet)" "Total Floorspace1" "Fewer than 500",3.2,0.9,0.5,0.4

  17. "Table HC12.2 Living Space Characteristics by Midwest Census Region, 2005"

    U.S. Energy Information Administration (EIA) Indexed Site

    2 Living Space Characteristics by Midwest Census Region, 2005" " Million U.S. Housing Units" ,,"Midwest Census Region" ,"U.S. Housing Units (millions)" ,,,"Census Division" ,,"Total Midwest" "Living Space Characteristics",,,"East North Central","West North Central" "Total",111.1,25.6,17.7,7.9 "Floorspace (Square Feet)" "Total Floorspace1" "Fewer than

  18. "Table HC13.2 Living Space Characteristics by South Census Region, 2005"

    U.S. Energy Information Administration (EIA) Indexed Site

    2 Living Space Characteristics by South Census Region, 2005" " Million U.S. Housing Units" ,,"South Census Region" ,"U.S. Housing Units (millions)" ,,,"Census Division" ,,"Total South" "Living Space Characteristics",,,"South Atlantic","East South Central","West South Central" "Total",111.1,40.7,21.7,6.9,12.1 "Floorspace (Square Feet)" "Total Floorspace1" "Fewer than

  19. "Table HC14.2 Living Space Characteristics by West Census Region, 2005"

    U.S. Energy Information Administration (EIA) Indexed Site

    2 Living Space Characteristics by West Census Region, 2005" " Million U.S. Housing Units" ,,"West Census Region" ,"U.S. Housing Units (millions)" ,,,"Census Division" ,,"Total West" "Living Space Characteristics",,,"Mountain","Pacific" "Total",111.1,24.2,7.6,16.6 "Floorspace (Square Feet)" "Total Floorspace1" "Fewer than 500",3.2,1,0.2,0.8 "500 to

  20. Five Ways SLAC's X-ray Laser Can Change the Way We Live: 'The First Five

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Years' Points to a Bright Future of High-impact Discovery at LCLS | Department of Energy Five Ways SLAC's X-ray Laser Can Change the Way We Live: 'The First Five Years' Points to a Bright Future of High-impact Discovery at LCLS Five Ways SLAC's X-ray Laser Can Change the Way We Live: 'The First Five Years' Points to a Bright Future of High-impact Discovery at LCLS March 11, 2016 - 11:29am Addthis News release from SLAC National Accelerator Laboratory, March 9, 2016 If you've ever stood in a

  1. LiveChat Thurs, 10/20, 2pm ET: Clean Tech Markets | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    LiveChat Thurs, 10/20, 2pm ET: Clean Tech Markets LiveChat Thurs, 10/20, 2pm ET: Clean Tech Markets October 17, 2011 - 11:30am Addthis Liisa O'Neill Liisa O'Neill Former New Media Specialist, Office of Public Affairs How can I participate? Send an email to newmedia@hq.doe.gov; Tweeting your question to @energy with the hashtag #energymatters; or leaving a question for Kauffman at Facebook.com/energygov. On Energy.gov, we've been showcasing a series of stories about innovations from our National

  2. LIVE Q&A TODAY: Answering Your Wind Energy Questions | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    LIVE Q&A TODAY: Answering Your Wind Energy Questions LIVE Q&A TODAY: Answering Your Wind Energy Questions August 8, 2013 - 12:03pm Addthis On Thursday, August 8, we hosted a Google+ Hangout on wind energy in America. Watch as our clean energy experts take your questions. Erin R. Pierce Erin R. Pierce Former Digital Communications Specialist, Office of Public Affairs How can I participate? Tweet questions to @ENERGY with the hashtag #AskEnergy. Ask us on Facebook and Google+. Email

  3. Tribal Summit Live: 9:30-10 AM ET Today and All Day Tomorrow | Department

    Energy Savers [EERE]

    of Energy Tribal Summit Live: 9:30-10 AM ET Today and All Day Tomorrow Tribal Summit Live: 9:30-10 AM ET Today and All Day Tomorrow May 4, 2011 - 8:00am Addthis Ginny Simmons Ginny Simmons Former Managing Editor for Energy.gov, Office of Public Affairs UPDATE: We've added video from the events. Indian Energy Tribal Summit with American Indian and Alaska Native leaders kicks off this morning in Arlington, Virginia, with welcoming remarks from ARPA-E Director and Senior Advisor to the

  4. Solar Decathlon Team Leading the Way Toward Sustainable Living, Even in the

    Energy Savers [EERE]

    Wake of Disasters | Department of Energy Team Leading the Way Toward Sustainable Living, Even in the Wake of Disasters Solar Decathlon Team Leading the Way Toward Sustainable Living, Even in the Wake of Disasters August 18, 2011 - 6:26pm Addthis The Re_house is nearing completion. | Courtesy of the University of Illinois at Urbana-Champaign Solar Decathlon Team. The Re_house is nearing completion. | Courtesy of the University of Illinois at Urbana-Champaign Solar Decathlon Team. April Saylor

  5. WEDNESDAY: Secretary Chu to Host Live Chat on Clean Energy and Innovation |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Secretary Chu to Host Live Chat on Clean Energy and Innovation WEDNESDAY: Secretary Chu to Host Live Chat on Clean Energy and Innovation January 24, 2011 - 12:00am Addthis WASHINGTON, D.C. - U.S. Energy Secretary Steven Chu will host the Department of Energy's inaugural "Energy Matters" online town hall on President Obama's clean energy and innovation agenda as a follow-up to the State of the Union. He will speak to and answer questions from an online and

  6. Ask the Directors: Secretary Chu to Host Live Chat on the Energy Innovation

    Energy Savers [EERE]

    Hubs | Department of Energy the Directors: Secretary Chu to Host Live Chat on the Energy Innovation Hubs Ask the Directors: Secretary Chu to Host Live Chat on the Energy Innovation Hubs March 5, 2012 - 12:32pm Addthis Secretary Chu is accepting questions on Facebook, Twitter and e-mail for the Energy Innovation Hub directors -- who are working to build better nuclear reactors, obtain fuel from sunlight and design the most energy efficient buildings to date. Michael Hess Michael Hess Former

  7. #askEnergy: Live Twitter Chat with A Solar Expert | Department of Energy

    Energy Savers [EERE]

    askEnergy: Live Twitter Chat with A Solar Expert #askEnergy: Live Twitter Chat with A Solar Expert June 5, 2012 - 11:51am Q&A What questions do you have about solar energy? Ask Us Addthis Photo by Dennis Schroeder/NREL. Photo by Dennis Schroeder/NREL. Erin R. Pierce Erin R. Pierce Former Digital Communications Specialist, Office of Public Affairs How can I participate? Join us for a Solar Twitter Chat this Friday, June 8 at 2 pm EDT. To participate, leave a comment on Facebook. Send

  8. Photo of the Week: Living Large -- Argonne's First Computer | Department of

    Energy Savers [EERE]

    Energy Living Large -- Argonne's First Computer Photo of the Week: Living Large -- Argonne's First Computer August 31, 2012 - 9:58am Addthis Before there was Google, or even the Internet, there was the computer -- and the earliest computers were so large that just one could occupy an entire room. AVIDAC was the first digital computer at Argonne National Laboratory, and began operating in 1953. It was built by the Physics Division for $250,000. Pictured here, with AVIDAC, is pioneer Argonne

  9. Five Efficient-Living Lessons from Student-Built Houses | Department of

    Office of Environmental Management (EM)

    Energy Five Efficient-Living Lessons from Student-Built Houses Five Efficient-Living Lessons from Student-Built Houses November 8, 2013 - 10:59am Addthis Team Austria of the Vienna Institute of Technology created an energy-efficient bathroom for Solar Decathlon 2013. | Photo from Jason Flakes/U.S. Department of Energy Solar Decathlon Team Austria of the Vienna Institute of Technology created an energy-efficient bathroom for Solar Decathlon 2013. | Photo from Jason Flakes/U.S. Department of

  10. DOE Tour of Zero: Options for Community Living by United Way of Long Island

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy Options for Community Living by United Way of Long Island DOE Tour of Zero: Options for Community Living by United Way of Long Island Addthis 1 of 14 United Way of Long Island Housing Development Corporation renovated this 1,436-square-foot home in Patchogue, New York, to the performance criteria of the U.S. Department of Energy Zero Energy Ready Home (ZERH) program. 2 of 14 The 1970s-era home had a HERS score of 162 in its pre-renovation condition, as shown here. When

  11. LiveChat Wed, 6/29, 2pm: Our Energy Independence | Department of Energy

    Office of Environmental Management (EM)

    6/29, 2pm: Our Energy Independence LiveChat Wed, 6/29, 2pm: Our Energy Independence June 27, 2011 - 9:29am Addthis Ginny Simmons Ginny Simmons Former Managing Editor for Energy.gov, Office of Public Affairs How can I participate? Submit a question via E-mail, Facebook or Twitter Watch live on June 29 at 2 PM ET As many of us hit the road to celebrate America's independence this upcoming 4th of July weekend, we must once again confront the reality of our country's economy, environment and

  12. Largest Solar Panel Installation at a U.S. University Goes Live |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Largest Solar Panel Installation at a U.S. University Goes Live Largest Solar Panel Installation at a U.S. University Goes Live November 4, 2010 - 6:10pm Addthis Sen. Menendez, Rep. Pascrell, John Lushetsky and other officials at the ribbon cutting. Sen. Menendez, Rep. Pascrell, John Lushetsky and other officials at the ribbon cutting. John Lushetsky Strategic Programs Director A couple of weeks ago, I had the opportunity to participate in a ribbon cutting event for the

  13. Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hill, Steven C.; Williamson, Chatt C.; Doughty, David C.; Pan, Yong-Le; Santarpia, Joshua L.; Hill, Hanna H.

    2015-02-02

    This paper uses a mathematical model of fluorescent biological particles composed of bacteria and/or proteins (mostly as in Hill et al., 2013 [23]) to investigate the size-dependence of the total fluorescence emitted in all directions. The model applies to particles which have negligible reabsorption of fluorescence within the particle. The specific particles modeled here are composed of ovalbumin and of a generic Bacillus. The particles need not be spherical, and in some cases need not be homogeneous. However, the results calculated in this paper are for spherical homogeneous particles. Light absorbing and fluorescing molecules included in the model are aminomore » acids, nucleic acids, and several coenzymes. Here the excitation wavelength is 266 nm. The emission range, 300 to 370 nm, encompasses the fluorescence of tryptophan. The fluorescence cross section (CF) is calculated and compared with one set of published measured values. We investigate power law (Ady) approximations to CF, where d is diameter, and A and y are parameters adjusted to fit the data, and examine how y varies with d and composition, including the fraction as water. The particle's fluorescence efficiency (QF=CF/geometric-cross-section) can be written for homogeneous particles as QabsRF, where Qabs is the absorption efficiency, and RF, the fraction of the absorbed light emitted as fluorescence, is independent of size and shape. When QF is plotted vs. mid or mi(mr-1)d, where m=mr+imi is the complex refractive index, the plots for different fractions of water in the particle tend to overlap.« less

  14. Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria

    SciTech Connect (OSTI)

    Hill, Steven C.; Williamson, Chatt C.; Doughty, David C.; Pan, Yong-Le; Santarpia, Joshua L.; Hill, Hanna H.

    2015-02-02

    This paper uses a mathematical model of fluorescent biological particles composed of bacteria and/or proteins (mostly as in Hill et al., 2013 [23]) to investigate the size-dependence of the total fluorescence emitted in all directions. The model applies to particles which have negligible reabsorption of fluorescence within the particle. The specific particles modeled here are composed of ovalbumin and of a generic Bacillus. The particles need not be spherical, and in some cases need not be homogeneous. However, the results calculated in this paper are for spherical homogeneous particles. Light absorbing and fluorescing molecules included in the model are amino acids, nucleic acids, and several coenzymes. Here the excitation wavelength is 266 nm. The emission range, 300 to 370 nm, encompasses the fluorescence of tryptophan. The fluorescence cross section (CF) is calculated and compared with one set of published measured values. We investigate power law (Ady) approximations to CF, where d is diameter, and A and y are parameters adjusted to fit the data, and examine how y varies with d and composition, including the fraction as water. The particle's fluorescence efficiency (QF=CF/geometric-cross-section) can be written for homogeneous particles as QabsRF, where Qabs is the absorption efficiency, and RF, the fraction of the absorbed light emitted as fluorescence, is independent of size and shape. When QF is plotted vs. mid or mi(mr-1)d, where m=mr+imi is the complex refractive index, the plots for different fractions of water in the particle tend to overlap.

  15. Live Status

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Software Policies User Surveys NERSC Users Group User Announcements Help Staff Blogs Request Repository Mailing List Operations for: Passwords & Off-Hours Status...

  16. EECBG Success Story: Making the "Best Place to Live" Even Better

    Broader source: Energy.gov [DOE]

    One advantage that Eden Prairie, Minnesota, will have going into next year’s Best Place to Live competition will be the energy efficiency upgrades taking place across the city thanks to $626,000 in Energy Efficiency and Conservation Block Grant (EECBG) funding provided through the American Recovery and Reinvestment Act. Learn more.

  17. Method of freezing living cells and tissues with improved subsequent survival

    DOE Patents [OSTI]

    Senkan, Selim M. (Oak Ridge, TN); Hirsch, Gerald P. (Oak Ridge, TN)

    1980-01-01

    This invention relates to an improved method for freezing red blood cells, ther living cells, or tissues with improved subsequent survival, wherein constant-volume freezing is utilized that results in significantly improved survival compared with constant-pressure freezing; optimization is attainable through the use of different vessel geometries, cooling baths and warming baths, and sample concentrations.

  18. ``Sleeping reactor`` irradiations: Shutdown reactor determination of short-lived activation products

    SciTech Connect (OSTI)

    Jerde, E.A.; Glasgow, D.C.

    1998-09-01

    At the High-Flux Isotope Reactor (HFIR) at the Oak Ridge National Laboratory, the principal irradiation system has a thermal neutron flux ({phi}) of {approximately} 4 {times} 10{sup 14} n/cm{sup 2} {center_dot} s, permitting the detection of elements via irradiation of 60 s or less. Irradiations of 6 or 7 s are acceptable for detection of elements with half-lives of as little as 30 min. However, important elements such as Al, Mg, Ti, and V have half-lives of only a few minutes. At HFIR, these can be determined with irradiation times of {approximately} 6 s, but the requirement of immediate counting leads to increased exposure to the high activity produced by irradiation in the high flux. In addition, pneumatic system timing uncertainties (about {+-} 0.5 s) make irradiations of < 6 s less reliable. Therefore, the determination of these ultra-short-lived species in mixed matrices has not generally been made at HFIR. The authors have found that very short lived activation products can be produced easily during the period after reactor shutdown (SCRAM), but prior to the removal of spent fuel elements. During this 24- to 36-h period (dubbed the ``sleeping reactor``), neutrons are produced in the beryllium reflector by the reaction {sup 9}Be({gamma},n){sup 8}Be, the gamma rays principally originating in the spent fuel. Upon reactor SCRAM, the flux drops to {approximately} 1 {times} 10{sup 10} n/cm{sup 2} {center_dot} s within 1 h. By the time the fuel elements are removed, the flux has dropped to {approximately} 6 {times} 10{sup 8}. Such fluxes are ideal for the determination of short-lived elements such as Al, Ti, Mg, and V. An important feature of the sleeping reactor is a flux that is not constant.

  19. Comparative genomic insights into ecophysiology of neutrophilic, microaerophilic iron oxidizing bacteria

    SciTech Connect (OSTI)

    Kato, Shingo; Ohkuma, Moriya; Powell, Deborah H.; Krepski, Sean T.; Oshima, Kenshiro; Hattori, Masahira; Shapiro, Nicole; Woyke, Tanja; Chan, Clara S.

    2015-11-13

    Neutrophilic microaerophilic iron-oxidizing bacteria (FeOB) are thought to play a significant role in cycling of carbon, iron and associated elements in both freshwater and marine iron-rich environments. However, the roles of the neutrophilic microaerophilic FeOB are still poorly understood due largely to the difficulty of cultivation and lack of functional gene markers. Here, we analyze the genomes of two freshwater neutrophilic microaerophilic stalk-forming FeOB, Ferriphaselus amnicola OYT1 and Ferriphaselus strain R-1. Phylogenetic analyses confirm that these are distinct species within Betaproteobacteria; we describe strain R-1 and propose the name F. globulitus. We compare the genomes to those of two freshwater Betaproteobacterial and three marine Zetaproteobacterial FeOB isolates in order to look for mechanisms common to all FeOB, or just stalk-forming FeOB. The OYT1 and R-1 genomes both contain homologs to cyc2, which encodes a protein that has been shown to oxidize Fe in the acidophilic FeOB, Acidithiobacillus ferrooxidans. This c-type cytochrome common to all seven microaerophilic FeOB isolates, strengthening the case for its common utility in the Fe oxidation pathway. In contrast, the OYT1 and R-1 genomes lack mto genes found in other freshwater FeOB. OYT1 and R-1 both have genes that suggest they can oxidize sulfur species. Both have the genes necessary to fix carbon by the Calvin–Benson– Basshom pathway, while only OYT1 has the genes necessary to fix nitrogen. The stalk-forming FeOB share xag genes that may help form the polysaccharide structure of stalks. Both OYT1 and R-1 make a novel biomineralization structure, short rod-shaped Fe oxyhydroxides much smaller than their stalks; these oxides are constantly shed, and may be a vector for C, P, and metal transport to downstream environments. Lastly, our results show that while different FeOB are adapted to particular niches, freshwater and marine FeOB likely share common mechanisms for Fe oxidation electron transport and biomineralization pathways.

  20. Comparative genomic insights into ecophysiology of neutrophilic, microaerophilic iron oxidizing bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Kato, Shingo; Ohkuma, Moriya; Powell, Deborah H.; Krepski, Sean T.; Oshima, Kenshiro; Hattori, Masahira; Shapiro, Nicole; Woyke, Tanja; Chan, Clara S.

    2015-11-13

    Neutrophilic microaerophilic iron-oxidizing bacteria (FeOB) are thought to play a significant role in cycling of carbon, iron and associated elements in both freshwater and marine iron-rich environments. However, the roles of the neutrophilic microaerophilic FeOB are still poorly understood due largely to the difficulty of cultivation and lack of functional gene markers. Here, we analyze the genomes of two freshwater neutrophilic microaerophilic stalk-forming FeOB, Ferriphaselus amnicola OYT1 and Ferriphaselus strain R-1. Phylogenetic analyses confirm that these are distinct species within Betaproteobacteria; we describe strain R-1 and propose the name F. globulitus. We compare the genomes to those of two freshwatermore » Betaproteobacterial and three marine Zetaproteobacterial FeOB isolates in order to look for mechanisms common to all FeOB, or just stalk-forming FeOB. The OYT1 and R-1 genomes both contain homologs to cyc2, which encodes a protein that has been shown to oxidize Fe in the acidophilic FeOB, Acidithiobacillus ferrooxidans. This c-type cytochrome common to all seven microaerophilic FeOB isolates, strengthening the case for its common utility in the Fe oxidation pathway. In contrast, the OYT1 and R-1 genomes lack mto genes found in other freshwater FeOB. OYT1 and R-1 both have genes that suggest they can oxidize sulfur species. Both have the genes necessary to fix carbon by the Calvin–Benson– Basshom pathway, while only OYT1 has the genes necessary to fix nitrogen. The stalk-forming FeOB share xag genes that may help form the polysaccharide structure of stalks. Both OYT1 and R-1 make a novel biomineralization structure, short rod-shaped Fe oxyhydroxides much smaller than their stalks; these oxides are constantly shed, and may be a vector for C, P, and metal transport to downstream environments. Lastly, our results show that while different FeOB are adapted to particular niches, freshwater and marine FeOB likely share common mechanisms for Fe oxidation electron transport and biomineralization pathways.« less

  1. The Effect of Bicarbonate on the Microbial Dissolution of Autunite Mineral in the Presence of Gram-Positive Bacteria

    SciTech Connect (OSTI)

    Sepulveda-Medina, Paola; Katsenovich, Yelena; Wellman, Dawn M.; Lagos, Leonel

    2015-06-01

    Bacteria are key players in the processes that govern fate and transport of contaminants. The uranium release from Na and Ca-autunite by Arthrobacter oxydans strain G968 was evaluated in the presence of bicarbonate ions. This bacterium was previously isolated from Hanford Site soil and in earlier prescreening tests demonstrated low tolerance to U(VI) toxicity compared to other A.oxydans isolates. Experiments were conducted using glass serum bottles as mixed bioreactors and sterile 6-well cell culture plates with inserts separating bacteria cells from mineral solids. Reactors containing phosphorus-limiting media were amended with bicarbonate ranging between 0-10 mM and metaautunite solids to provide a U(VI) concentration of 4.4 mmol/L. Results showed that in the presence of bicarbonate, A.oxydans G968 was able to enhance the release of U(VI) from Na and Ca autunite at the same capacity as other A.oxydans isolates with relatively high tolerance to U(VI). The effect of bacterial strains on autunite dissolution decreases as the concentration of bicarbonate increases. The results illustrate that direct interaction between the bacteria and the mineral is not necessary to result in U (VI) biorelease from autunite. The formation of secondary calcium-phosphate mineral phases on the surface of the mineral during the dissolution can ultimately reduce the natural autunite mineral contact area, which bacterial cells can access. This thereby reduces the concentration of uranium released into the solution. This study provides a better understanding of the interactions between meta-autunite and microbes in conditions mimicking arid and semiarid subsurface environments of western U.S.

  2. FRACTURE MECHANICS APPROACH TO ESTIMATE FATIGUE LIVES OF WELDED LAP-SHEAR SPECIMENS

    SciTech Connect (OSTI)

    Lam, P.; Michigan, J.

    2014-04-25

    A full range of stress intensity factor solutions for a kinked crack is developed as a function of weld width and the sheet thickness. When used with the associated main crack solutions (global stress intensity factors) in terms of the applied load and specimen geometry, the fatigue lives can be estimated for the laser-welded lap-shear specimens. The estimations are in good agreement with the experimental data. A classical solution for an infinitesimal kink is also employed in the approach. However, the life predictions tend to overestimate the actual fatigue lives. The traditional life estimations with the structural stress along with the experimental stress-fatigue life data (S-N curve) are also provided. In this case, the estimations only agree with the experimental data under higher load conditions.

  3. Long-Range Untethered Real-Time Live Gas Main Robotic Inspection System

    SciTech Connect (OSTI)

    Hagen Schempf; Daphne D'Zurko

    2004-10-31

    Under funding from the Department of Energy (DOE) and the Northeast Gas Association (NGA), Carnegie Mellon University (CMU) developed an untethered, wireless remote controlled inspection robot dubbed Explorer. The project entailed the design and prototyping of a wireless self-powered video-inspection robot capable of accessing live 6- and 8-inch diameter cast-iron and steel mains, while traversing turns and Ts and elbows under real-time control with live video feedback to an operator. The design is that of a segmented actively articulated and wheel-leg powered robot design, with fisheye imaging capability and self-powered battery storage and wireless real-time communication link. The prototype was functionally tested in an above ground pipe-network, in order to debug all mechanical, electrical and software subsystems, and develop the necessary deployment and retrieval, as well as obstacle-handling scripts. A pressurized natural gas test-section was used to certify it for operation in natural gas at up to 60 psig. Two subsequent live-main field-trials in both cast-iron and steel pipe, demonstrated its ability to be safely launched, operated and retrieved under real-world conditions. The system's ability to safely and repeatably exidrecover from angled and vertical launchers, traverse multi-thousand foot long pipe-sections, make T and varied-angle elbow-turns while wirelessly sending live video and handling command and control messages, was clearly demonstrated. Video-inspection was clearly shown to be a viable tool to understand the state of this critical buried infrastructure, irrespective of low- (cast-iron) or high-pressure (steel) conditions. This report covers the different aspects of specifications, requirements, design, prototyping, integration and testing and field-trialing of the Explorer platform.

  4. Imaging single cells in a beam of live cyanobacteria with an X-ray laser

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Schot, Gijs, vander

    2015-02-10

    This entry contains ten diffraction patterns, and reconstructions images, of individual living Cyanobium gracile cells, imaged using 517 eV X-rays from the LCLS XFEL. The Hawk software package was used for phasing. The Uppsala aerosol injector was used for sample injection, assuring very low noise levels. The cells come from various stages of the cell cycle, and were imaged in random orientations.

  5. "Table HC1.2.3 Living Space Characteristics by Average Floorspace--"

    U.S. Energy Information Administration (EIA) Indexed Site

    3 Living Space Characteristics by Average Floorspace--" " Single-Family Housing Units and Mobile Homes, 2005" ,,"Single- Family and Mobile Homes (millions)","Average Square Feet per Housing Unit" ," Housing Units (millions)" ,,,"Single-Family Detached",,,"Single-Family Attached",,,"Mobile Homes" "Housing Unit

  6. Feb. 7 Science Series Lecturer to Discuss Living & Working in the Arctic |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Jefferson Lab 7 Science Series Lecturer to Discuss Living & Working in the Arctic NEWPORT NEWS, VA, Jan. 19, 2012 - The Thomas Jefferson National Accelerator Facility hosts its next Science Series lecture on Tuesday, Feb. 7, with a discussion about carrying out research in a freezer - the extreme cold of the Arctic. Guest speaker Victoria Hill, an oceanographer with Old Dominion University's bio-optics group, will talk about the work she and colleagues have undertaken to try to explain

  7. In situ Observation of Sulfur in Living Mammalian Cells: Uptake of Taurine

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    into MDCK Cells In situ Observation of Sulfur in Living Mammalian Cells: Uptake of Taurine into MDCK Cells Sulfur is essential for life. It plays important roles in the amino acids methionine and cysteine, and has a structural function in disulfide bonds. As a component of iron-sulfur clusters it takes part in electron and sulfur transfer reactions.1 Glutathione, a sulfur-containing tripeptide, is an important part of biological antioxidant systems.2 Another example for the biological

  8. Savannah River Site "Live Burn" Training Sharpens Skills | National Nuclear

    National Nuclear Security Administration (NNSA)

    Security Administration "Live Burn" Training Sharpens Skills | National Nuclear Security Administration Facebook Twitter Youtube Flickr RSS People Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Countering Nuclear Terrorism About Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Library Bios Congressional Testimony Fact Sheets Newsletters Press Releases Photo

  9. Live Q&A: Celebrating Black History Month and STEM Education | Department

    Office of Environmental Management (EM)

    of Energy Q&A: Celebrating Black History Month and STEM Education Live Q&A: Celebrating Black History Month and STEM Education February 22, 2013 - 3:14pm Q&A What questions do you have about STEM education & careers? Ask our experts Addthis Join our Google+ Hangout on increasing diversity in science, technology, engineering & math disciplines. | Infographic by Sarah Gerrity. Join our Google+ Hangout on increasing diversity in science, technology, engineering & math

  10. Tracking chemical changes in a live cell: Biomedical applications of SR-FTIR spectromicroscopy

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Holman, Hoi-Ying N.; Martin, Michael C.; McKinney, Wayne R.

    2003-01-01

    Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectromicroscopy is a newly emerging bioanalytical and imaging tool. This unique technique provides mid-infrared (IR) spectra, hence chemical information, with high signal-to-noise at spatial resolutions as fine as 3 to 10 microns. Thus it enables researchers to locate, identify, and track specific chemical events within an individual living mammalian cell. Mid-IR photons are too low in energy (0.05-0.5 eV) to either break bonds or to cause ionization. In this review, we show that the synchrotron IR beam has no detectable effects on the short- and long-term viability, reproductive integrity, cell-cycle progression, and mitochondrial metabolismmore » in living human cells, and produces only minimal sample heating (<0.5°C). We will then present several examples demonstrating the application potentials of SR-FTIR spectromicroscopy in biomedical research. These will include monitoring living cells progressing through the cell cycle, including death, and cells reacting to dilute concentrations of toxins.« less

  11. Ultrafast nanolaser device for detecting cancer in a single live cell.

    SciTech Connect (OSTI)

    Gourley, Paul Lee; McDonald, Anthony Eugene

    2007-11-01

    Emerging BioMicroNanotechnologies have the potential to provide accurate, realtime, high throughput screening of live tumor cells without invasive chemical reagents when coupled with ultrafast laser methods. These optically based methods are critical to advancing early detection, diagnosis, and treatment of disease. The first year goals of this project are to develop a laser-based imaging system integrated with an in- vitro, live-cell, micro-culture to study mammalian cells under controlled conditions. In the second year, the system will be used to elucidate the morphology and distribution of mitochondria in the normal cell respiration state and in the disease state for normal and disease states of the cell. In this work we designed and built an in-vitro, live-cell culture microsystem to study mammalian cells under controlled conditions of pH, temp, CO2, Ox, humidity, on engineered material surfaces. We demonstrated viability of cell culture in the microsystem by showing that cells retain healthy growth rates, exhibit normal morphology, and grow to confluence without blebbing or other adverse influences of the material surfaces. We also demonstrated the feasibility of integrating the culture microsystem with laser-imaging and performed nanolaser flow spectrocytometry to carry out analysis of the cells isolated mitochondria.

  12. Mechanical properties of interacting lipopolysaccharide membranes from bacteria mutants studied by specular and off-specular neutron scattering

    SciTech Connect (OSTI)

    Schneck, Emanuel; Tanaka, Motomu; Oliveira, Rafael G.; Rehfeldt, Florian; Deme, Bruno; Brandenburg, Klaus; Seydel, Ulrich

    2009-10-15

    Specular and off-specular neutron scattering are used to study the influence of molecular chemistry (mutation) on the intermembrane interactions and mechanical properties of the outer membrane of Gram-negative bacteria consisting of lipopolysaccharides (LPSs). For this purpose, solid-supported multilayers of mutant LPS membranes are deposited on silicon wafers and hydrated either at defined humidity or in bulk buffers. The planar sample geometry allows to identify out-of-plane and in-plane scattering vector components. The measured two-dimensional reciprocal space maps are simulated with membrane displacement correlation functions determined by two mechanical parameters (vertical compression modulus and bending rigidity) and an effective cutoff radius for the membrane fluctuation wavelength. Experiments at controlled humidity enable one to examine the influence of the disjoining pressure on the saccharide-mediated intermembrane interactions, while experiments in bulk buffers (i.e., in the absence of an external osmotic stress) reveal the effect of divalent cations on LPS membranes, highlighting the role of divalent cations in the survival mechanism of bacteria in the presence of antimicrobial molecules.

  13. Use of bromodeoxyuridine immunocapture to identify psychrotolerant phenanthrene-degrading bacteria in phenanthrene-enriched polluted Baltic Sea sediments

    SciTech Connect (OSTI)

    Edlund, A.; Jansson, J.

    2008-05-01

    The aim of this study was to enrich and identify psychrotolerant phenanthrenedegrading bacteria from polluted Baltic Sea sediments. Polyaromatic hydrocarbon (PAH)-contaminated sediments were spiked with phenanthrene and incubated for 2 months in the presence of bromodeoxyuridine that is incorporated into the DNA of replicating cells. The bromodeoxyuridine-incorporated DNA was extracted by immunocapture and analyzed by terminal-restriction fragment length polymorphism and 16S rRNA gene cloning and sequencing to identify bacterial populations that were growing. In addition, degradation genes were quantified in the bromodeoxyuridine-incorporated DNA by real-time PCR. Phenanthrene concentrations decreased after 2 months of incubation in the phenanthrene-enriched sediments and this reduction correlated to increases in copy numbers of xylE and phnAc dioxygenase genes. Representatives of Exiguobacterium, Schewanella,Methylomonas, Pseudomonas, Bacteroides and an uncultured Deltaproteobacterium and a Gammaproteobacterium dominated the growing community in the phenanthrene spiked sediments. Isolates that were closely related to three of these bacteria (two pseudomonads and an Exiguobacterium sp.) could reduce phenanthrene concentrations in pure cultures and they all harbored phnAc dioxygenase genes. These results confirm that this combination of culture-based and molecular approaches was useful for identification of actively growing bacterial species with a high potential for phenanthrene degradation.

  14. Developing new optical imaging techniques for single particle and molecule tracking in live cells

    SciTech Connect (OSTI)

    Sun, Wei

    2010-12-15

    Differential interference contrast (DIC) microscopy is a far-field as well as wide-field optical imaging technique. Since it is non-invasive and requires no sample staining, DIC microscopy is suitable for tracking the motion of target molecules in live cells without interfering their functions. In addition, high numerical aperture objectives and condensers can be used in DIC microscopy. The depth of focus of DIC is shallow, which gives DIC much better optical sectioning ability than those of phase contrast and dark field microscopies. In this work, DIC was utilized to study dynamic biological processes including endocytosis and intracellular transport in live cells. The suitability of DIC microscopy for single particle tracking in live cells was first demonstrated by using DIC to monitor the entire endocytosis process of one mesoporous silica nanoparticle (MSN) into a live mammalian cell. By taking advantage of the optical sectioning ability of DIC, we recorded the depth profile of the MSN during the endocytosis process. The shape change around the nanoparticle due to the formation of a vesicle was also captured. DIC microscopy was further modified that the sample can be illuminated and imaged at two wavelengths simultaneously. By using the new technique, noble metal nanoparticles with different shapes and sizes were selectively imaged. Among all the examined metal nanoparticles, gold nanoparticles in rod shapes were found to be especially useful. Due to their anisotropic optical properties, gold nanorods showed as diffraction-limited spots with disproportionate bright and dark parts that are strongly dependent on their orientation in the 3D space. Gold nanorods were developed as orientation nanoprobes and were successfully used to report the self-rotation of gliding microtubules on kinesin coated substrates. Gold nanorods were further used to study the rotational motions of cargoes during the endocytosis and intracellular transport processes in live mammalian cells. New rotational information was obtained: (1) during endocytosis, cargoes lost their rotation freedom at the late stage of internalization; (2) cargoes performed train-like motion when they were transported along the microtubule network by motor proteins inside live cells; (3) During the pause stage of fast axonal transport, cargoes were still bound to the microtubule tracks by motor proteins. Total internal reflection fluorescence microscopy (TIRFM) is another non-invasive and far-field optical imaging technique. Because of its near-field illumination mechanism, TIRFM has better axial resolution than epi-fluorescence microscopy and confocal microscopy. In this work, an auto-calibrated, prism type, angle-scanning TIRFM instrument was built. The incident angle can range from subcritical angles to nearly 90{sup o}, with an angle interval less than 0.2{sup o}. The angle precision of the new instrument was demonstrated through the finding of the surface plasmon resonance (SPR) angle of metal film coated glass slide. The new instrument improved significantly the precision in determining the axial position. As a result, the best obtained axial resolution was {approx} 8 nm, which is better than current existing instruments similar in function. The instrument was further modified to function as a pseudo TIRF microscope. The illumination depth can be controlled by changing the incident angle of the excitation laser beam or adjusting the horizontal position of the illumination laser spot on the prism top surface. With the new technique, i.e., variable-illumination-depth pseudo TIRF microscopy, the whole cell body from bottom to top was scanned.

  15. Microorganism genomics, compositions and methods related thereto

    DOE Patents [OSTI]

    Handelsman, Jo (Madison, WI); Goodman, Robert M. (Madison, WI); Rondon, Michelle R. (Madison, WI)

    2001-01-01

    The present invention provides methods and compositions for accessing, in a generally unbaised manner, a diverse genetic pool for genes involved in biosynthetic pathways. The invention also provides compounds which can be identified by cloning biosynthetic pathways.

  16. System, device, and methods for real-time screening of live cells, biomarkers, and chemical signatures

    DOE Patents [OSTI]

    Sundaram, S Kamakshi [Richland, WA; Riley, Brian J [West Richland, WA; Weber, Thomas J [Richland, WA; Sacksteder, Colette A [West Richland, WA; Addleman, R Shane [Benton City, WA

    2011-06-07

    An ATR-FTIR device and system are described that defect live-cell responses to stimuli and perturbations in real-time. The system and device can monitor perturbations resulting from exposures to various physical, chemical, and biological materials in real-time, as well as those sustained over a long period of time, including those associated with stimuli having unknown modes-of-action (e.g. nanoparticles). The device and system can also be used to identify specific chemical species or substances that profile cellular responses to these perturbations.

  17. Table HC6.2 Living Space Characteristics by Number of Household Members, 2005

    Gasoline and Diesel Fuel Update (EIA)

    2 Living Space Characteristics by Number of Household Members, 2005 Total...................................................................... 111.1 30.0 34.8 18.4 15.9 12.0 Floorspace (Square Feet) Total Floorspace 1 Fewer than 500............................................... 3.2 1.7 0.8 0.4 0.3 Q 500 to 999....................................................... 23.8 10.2 6.4 3.4 2.3 1.5 1,000 to 1,499................................................. 20.8 5.5 6.3 3.0 3.3 2.6 1,500 to

  18. Installation of 2 7/8-in. coiled-tubing tailpipes in live gas wells

    SciTech Connect (OSTI)

    Campbell, J.A.; Bayes, K.P.

    1994-05-01

    This paper describes a technique for installing 2 7/8-in. coiled tubing as tailpipe extensions below existing production packers in live gas wells. It also covers the use of coiled tubing as a way to complete wells. Large savings in rig time and deferred production have been realized with this technique. Fluid losses to the formation do not occur, and no expensive rig time is needed to kill or clean up the wells, as required for conventional workovers below existing production packers. This technique is particularly applicable in depleted reservoirs that could be impaired by traditional workover methods.

  19. LiveChat Wed, 11/16, 2 pm ET: Industrial Energy Efficiency | Department of

    Office of Environmental Management (EM)

    Energy 11/16, 2 pm ET: Industrial Energy Efficiency LiveChat Wed, 11/16, 2 pm ET: Industrial Energy Efficiency November 14, 2011 - 1:40pm Addthis Do you have questions or ideas about how the U.S. Department of Energy can contribute to global competitiveness through industrial efficiency? Dr. Kathleen Hogan would like to hear them. Michael Hess Michael Hess Former Digital Communications Specialist, Office of Public Affairs How can I participate? Send an email to Dr. Hogan at

  20. Electric and magnetic field exposures for people living near a 735-Kilovolt power line

    SciTech Connect (OSTI)

    Levallois, P.; Gauvin, D.; St. Laurent, J.

    1995-09-01

    The purpose of this study was to assess the effect of a 735-kV transmission line on the electric and magnetic field exposures of people living at the edge of the line`s right of way. Exposure of 18 adults, mostly white-collar workers, living in different bungalows located 190-240 feet from the line (exposed subjects) was compared to that of 17 adults living in similar residences far away from any transmission line. Each subject carried a Positron meter for 24 hr during 1 workday, which measured 60-Hz electric and magnetic fields every minute. All measurements were carried out in parallel for exposed and unexposed subjects during the same weeks between September and December. During measurements the average loading on the line varied between 600 and 1100 A. The average magnetic field intensity while at home was 4.4 times higher among exposed subjects than unexposed (7.1 versus 1.6 Mg, p=0.0001) and 6.2 times higher when considering only the sleeping period (6.8 versus 1.1 mG, p=0.0001). Based on the 24-hr measurement, average magnetic field exposure was three times higher among the exposed was positively correlated with the loading on the line (r=0.8, p+0.001). Percentage of time above a magnetic field threshold F(2 mG or 7.8 mG) was a good indicator to distinguish the two types of exposure. Percentage of time above 20 V/m was significantly different, but percentage of time above 78 V/m was rare and comparable for the two groups. Variability of exposure was very low. This study demonstrates that a 735-kV line contributes significantly to residential 60-Hz magnetic field exposure and, to a lesser extent, electric fields for people living at the edge of the right way. Because of the limited size of our sample, caution is recommended before generalizing these results. Nevertheless, due to the uncertainty on the risks associated with such an unusual high residential exposure, research is needed on its possible effects. 30 refs., 1 fig., 7 tabs.

  1. Acting Biomass Program Manager Dr. Valerie Reed to Host Live Twitter Q&A on Advanced Biofuels

    Broader source: Energy.gov [DOE]

    Washington, D.C. – On Friday, December 16th, the Energy Department (@energy) will be hosting a live Twitter Q&A on biofuels with Dr. Valerie Reed, Acting Manager of the Biomass Program.

  2. Manila clams from Hg polluted sediments of Marano and Grado lagoons (Italy) harbor detoxifying Hg resistant bacteria in soft tissues

    SciTech Connect (OSTI)

    Baldi, Franco; Gallo, Michele; Marchetto, Davide; Faleri, Claudia; Maida, Isabel; Fani, Renato

    2013-08-15

    A mechanism of mercury detoxification has been suggested by a previous study on Hg bioaccumulation in Manila clams (Ruditapes philippinarum) in the polluted Marano and Grado lagoons and in this study we demonstrate that this event could be partly related to the detoxifying activities of Hg-resistant bacteria (MRB) harbored in clam soft tissues. Therefore, natural clams were collected in six stations during two different periods (winter and spring) from Marano and Grado Lagoons. Siphons, gills and hepatopancreas from acclimatized clams were sterile dissected to isolate MRB. These anatomical parts were glass homogenized or used for whole, and they were lying on a solid medium containing 5 mg l{sup −1} HgCl{sub 2} and incubated at 30 °C. A total of fourteen bacterial strains were isolated and were identified by 16S rDNA sequencing and analysis, revealing that strains were representative of eight bacterial genera, four of which were Gram-positive (Enterococcus, Bacillus, Jeotgalicoccus and Staphylococcus) and other four were Gram-negative (Stenotrophomonas, Vibrio, Raoultella and Enterobacter). Plasmids and merA genes were found and their sequences determined. Fluorescence in situ hybridization (FISH) technique shows the presence of Firmicutes, Actinobacteria and Gammaproteobacteria by using different molecular probes in siphon and gills. Bacterial clumps inside clam flesh were observed and even a Gram-negative endosymbiont was disclosed by transmission electronic microscope inside clam cells. Bacteria harbored in cavities of soft tissue have mercury detoxifying activity. This feature was confirmed by the determination of mercuric reductase in glass-homogenized siphons and gills. -- Highlights: ► We isolated Gram-positive and Gram-negative Hg resistant strains from soft tissues of Ruditapes philippinarum. ► We identify 14 mercury resistant strains by 16S rRNA gene sequences. ► Bacteria in siphon and gill tissues of clams were observed by TEM and identified with different FISH probes. ► Hg-reductase (MerA) activity in glass homogenized clam tissues was also determined.

  3. Fixed Monthly Living Expense Payments at the Lawrence Livermore National Laboratory, INS-L-11-05

    Office of Environmental Management (EM)

    Fixed Monthly Living Expense Payments at the Lawrence Livermore National Laboratory INS-L-11-05 September 2011 Department of Energy Washington, DC 20585 September 21, 2011 MEMORANDUM FOR MANAGER, LIVERMORE SITE OFFICE FROM: Sandra D. Bruce Assistant Inspector General for Inspections SUBJECT: INFORMATION: Inspection Report on "Fixed Monthly Living Expense Payments at the Lawrence Livermore National Laboratory" BACKGROUND The Lawrence Livermore National Laboratory (Livermore) is a

  4. Method for detection of long-lived radioisotopes in small biochemical samples

    DOE Patents [OSTI]

    Turteltaub, Kenneth W.; Vogel, John S.; Felton, James S.; Gledhill, Barton L.; Davis, Jay C.

    1994-01-01

    Disclosed is a method for detection of long-lived radioisotopes in small bio-chemical samples, comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering said chemical specie to said biologist host in doses sufficiently low to avoid significant overt damage to the biological system thereof, d. allowing a period of time to elapse sufficient for dissemination and interaction of said chemical specie with said host throughout said biological system of said host, e. isolating a reacted fraction of the biological substance from said host in a manner sufficient to avoid contamination of said substance from extraneous sources, f. converting said fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in said material by means of direct isotopic counting.

  5. Method for detection of long-lived radioisotopes in small biochemical samples

    DOE Patents [OSTI]

    Turteltaub, K.W.; Vogel, J.S.; Felton, J.S.; Gledhill, B.L.; Davis, J.C.

    1994-11-22

    Disclosed is a method for detection of long-lived radioisotopes in small biochemical samples, comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering the chemical specie to the biologist host in doses sufficiently low to avoid significant overt damage to the biological system, d. allowing a period of time to elapse sufficient for dissemination and interaction of the chemical specie with the host throughout the biological system of the host, e. isolating a reacted fraction of the biological substance from the host in a manner sufficient to avoid contamination of the substance from extraneous sources, f. converting the fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in the material by means of direct isotopic counting. 5 figs.

  6. Short-lived pollutants in the Arctic: their climate impact and possible mitigation strategies

    SciTech Connect (OSTI)

    Menon, Surabi; Quinn, P.K.; Bates, T.S.; Baum, E.; Doubleday, N.; Fiore, A.M.; Flanner, M.; Fridlind, A.; Garrett, T.J.; Koch, D.; Menon, S.; Shindell, D.; Stohl, A.; Warren, S.G.

    2007-09-24

    Several short-lived pollutants known to impact Arctic climate may be contributing to the accelerated rates of warming observed in this region relative to the global annually averaged temperature increase. Here, we present a summary of the short-lived pollutants that impact Arctic climate including methane, tropospheric ozone, and tropospheric aerosols. For each pollutant, we provide a description of the major sources and the mechanism of forcing. We also provide the first seasonally averaged forcing and corresponding temperature response estimates focused specifically on the Arctic. The calculations indicate that the forcings due to black carbon, methane, and tropospheric ozone lead to a positive surface temperature response indicating the need to reduce emissions of these species within and outside the Arctic. Additional aerosol species may also lead to surface warming if the aerosol is coincident with thin, low lying clouds. We suggest strategies for reducing the warming based on current knowledge and discuss directions for future research to address the large remaining uncertainties.

  7. Testing, Modeling, and Monitoring to Enable Simpler, Cheaper, Longer-Lived Surface Caps

    SciTech Connect (OSTI)

    Piet, Steven James; Breckenridge, Robert Paul; Burns, Douglas Edward

    2003-02-01

    Society has and will continue to generate hazardous wastes whose risks must be managed. For exceptionally toxic, long-lived, and feared waste, the solution is deep burial, e.g., deep geological disposal at Yucca Mtn. For some waste, recycle or destruction/treatment is possible. The alternative for other wastes is storage at or near the ground level (in someones back yard); most of these storage sites include a surface barrier (cap) to prevent downward water migration. Some of the hazards will persist indefinitely. As society and regulators have demanded additional proof that caps are robust against more threats and for longer time periods, the caps have become increasingly complex and expensive. As in other industries, increased complexity will eventually increase the difficulty in estimating performance, in monitoring system/component performance, and in repairing or upgrading barriers as risks are managed. An approach leading to simpler, less expensive, longer-lived, more manageable caps is needed. Our project, which started in April 2002, aims to catalyze a Barrier Improvement Cycle (iterative learning and application) and thus enable Remediation System Performance Management (doing the right maintenance neither too early nor too late). The knowledge gained and the capabilities built will help verify the adequacy of past remedial decisions, improve barrier management, and enable improved solutions for future decisions. We believe it will be possible to develop simpler, longer-lived, less expensive caps that are easier to monitor, manage, and repair. The project is planned to: a) improve the knowledge of degradation mechanisms in times shorter than service life; b) improve modeling of barrier degradation dynamics; c) develop sensor systems to identify early degradation; and d) provide a better basis for developing and testing of new barrier systems. This project combines selected exploratory studies (benchtop and field scale), coupled effects accelerated aging testing at the intermediate meso-scale, testing of new monitoring concepts, and modeling of dynamic systems. The emphasis on meso-scale (coupled) tests, accelerated effects testing, and dynamic modeling differentiates the project from other efforts, while simultaneously building on that body of knowledge. The performance of evapotranspiration, capillary, and grout-based barriers is being examined. To date, the project can report new approaches to the problem, building new experimental and modeling capabilities, and a few preliminary results.

  8. Testing, Modeling, and Monitoring to Enable Simpler, Cheaper, Longer-lived Surface Caps

    SciTech Connect (OSTI)

    Piet, S. J.; Breckenridge, R. P.; Burns, D. E.

    2003-02-25

    Society has and will continue to generate hazardous wastes whose risks must be managed. For exceptionally toxic, long-lived, and feared waste, the solution is deep burial, e.g., deep geological disposal at Yucca Mtn. For some waste, recycle or destruction/treatment is possible. The alternative for other wastes is storage at or near the ground level (in someone's back yard); most of these storage sites include a surface barrier (cap) to prevent downward water migration. Some of the hazards will persist indefinitely. As society and regulators have demanded additional proof that caps are robust against more threats and for longer time periods, the caps have become increasingly complex and expensive. As in other industries, increased complexity will eventually increase the difficulty in estimating performance, in monitoring system/component performance, and in repairing or upgrading barriers as risks are managed. An approach leading to simpler, less expensive, longer-lived, more manageable caps is needed. Our project, which started in April 2002, aims to catalyze a Barrier Improvement Cycle (iterative learning and application) and thus enable Remediation System Performance Management (doing the right maintenance neither too early nor too late). The knowledge gained and the capabilities built will help verify the adequacy of past remedial decisions, improve barrier management, and enable improved solutions for future decisions. We believe it will be possible to develop simpler, longer-lived, less expensive caps that are easier to monitor, manage, and repair. The project is planned to: (a) improve the knowledge of degradation mechanisms in times shorter than service life; (b) improve modeling of barrier degradation dynamics; (c) develop sensor systems to identify early degradation; and (d) provide a better basis for developing and testing of new barrier systems. This project combines selected exploratory studies (benchtop and field scale), coupled effects accelerated aging testing at the intermediate meso-scale, testing of new monitoring concepts, and modeling of dynamic systems. The emphasis on meso-scale (coupled) tests, accelerated effects testing, and dynamic modeling differentiates the project from other efforts, while simultaneously building on that body of knowledge. The performance of evapotranspiration, capillary, and grout-based barriers is being examined. To date, the project can report new approaches to the problem, building new experimental and modeling capabilities, and a few preliminary results.

  9. Living in a Materials World: Materials Science Engineering Professional Development for K-12 Educators

    SciTech Connect (OSTI)

    Anne Seifert; Louis Nadelson

    2011-06-01

    Advances in materials science are fundamental to technological developments and have broad societal impacs. For example, a cellular phone is composed of a polymer case, liquid crystal displays, LEDs, silicon chips, Ni-Cd batteries, resistors, capacitors, speakers, microphones all of which have required advances in materials science to be compacted into a phone which is typically smaller than a deck of cards. Like many technological developments, cellular phones have become a ubiquitous part of society, and yet most people know little about the materials science associated with their manufacture. The probable condition of constrained knowledge of materials science was the motivation for developing and offering a 20 hour fourday course called 'Living in a Materials World.' In addition, materials science provides a connection between our every day experiences and the work of scientists and engineers. The course was offered as part of a larger K-12 teacher professional development project and was a component of a week-long summer institute designed specifically for upper elementary and middle school teachers which included 20 hour content strands, and 12 hours of plenary sessions, planning, and collaborative sharing. The focus of the institute was on enhancing teacher content knowledge in STEM, their capacity for teaching using inquiry, their comfort and positive attitudes toward teaching STEM, their knowledge of how people learn, and strategies for integrating STEM throughout the curriculum. In addition to the summer institute the participating teachers were provided with a kit of about $300 worth of materials and equipment to use to implement the content they learned in their classrooms. As part of this professional development project the participants were required to design and implement 5 lesson plans with their students this fall and report on the results, as part of the continuing education course associated with the project. 'Living in a Materials World' was one of the fifteen content strands offered at the institute. The summer institute participants were pre/post tested on their comfort with STEM, their perceptions of STEM education, their pedagogical discontentment, their implementations of inquiry, their attitudes toward student learning of STEM, and their content knowledge associated with their specific content strand. The results from our research indicate a significant increase in content knowledge (t = 11.36, p < .01) for the Living in a Materials World strand participants. Overall the summer institute participants were found to have significant increases in their comfort levels for teaching STEM (t = 10.94, p < .01), in inquiry implementation (t = 5.72, p < .01) and efficacy for teaching STEM (t = 6.27, p < .01) and significant decrease in pedagogical discontentment (t = -6.26, p < .01).

  10. Preliminary investigation of the effects of mineralogy and fluid composition on the growth of thermophilic bacteria in geothermal hot springs on the island of Vulcano, Italy

    SciTech Connect (OSTI)

    Amend, J.P.; Helgeson, H.C. . Dept. of Geology and Geophysics); Gurrieri, S.; Valenza, M. ); Clark, D.S. . Dept. of Chemical Engineering)

    1992-01-01

    Hydrothermal experiments were carried out recently on the island of Vulcano to investigate at in situ temperatures the relation of thermophilic bacterial growth to the mineralogy and fluid chemistry of geothermal hot springs. A preheated nutrient medium was inoculated with geothermal fluid and placed in the hydrothermal reactor, together with a sample of the mineralogic matrix through which the fluid flows. The results of the experiments are somewhat equivocal owing to (1) the inability to maintain the pH of the reactor fluid at the in situ pH (2.9 at 98 C), (2) apparent phase separation of what is probably a CO[sub 2]-rich gas leading to abnormally high pressures as the reactor temperature was increased in stages to 125 C, and (3) the fact that (unexpectedly) all of the bacteria were found to occur on the surfaces of mineral grains, which could not be sequentially collected in a representative manner with the apparatus at hand. Nevertheless, it appeared qualitatively that the population of bacteria increased during the experiment. Although this observation requires future confirmation and quantification with a more sophisticated reactor, the experimental results clearly indicate that conventional microbiological growth experiments using thermophilic bacteria that have been removed from their natural nutrient, in situ pH, and mineralogic environment may have little to do with the behavior of such bacteria in geothermal systems. Understanding this behavior requires integrated studies of the organobiogeochemistry of geothermal systems.

  11. Processing of cellulosic material by a cellulase-containing cell-free fermentate produced from cellulase-producing bacteria, ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1998-08-04

    Bacteria which produce large amounts of a cellulase-containing cell-free fermentate, have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase degrading bacterium ATCC 55702, which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic materials. 5 figs.

  12. Processing of cellulosic material by a cellulase-containing cell-free fermentate produced from cellulase-producing bacteria, ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig (Lenoir City, TN)

    1998-01-01

    Bacteria which produce large amounts of a cellulase-containing cell-free fermentate, have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase degrading bacterium ATCC 55702, which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic materials.

  13. Long-lived light mediator to dark matter and primordial small scale spectrum

    SciTech Connect (OSTI)

    Zhang, Yue

    2015-05-06

    We calculate the early universe evolution of perturbations in the dark matter energy density in the context of simple dark sector models containing a GeV scale light mediator. We consider the case that the mediator is long-lived, with lifetime up to a second, and before decaying it temporarily dominates the energy density of the universe. We show that for primordial perturbations that enter the horizon around this period, the interplay between linear growth during matter domination and collisional damping can generically lead to a sharp peak in the spectrum of dark matter density perturbation. As a result, the population of the smallest DM halos gets enhanced. Possible implications of this scenario are discussed.

  14. Removal of long-lived {sup 222}Rn daughters by electropolishing thin layers of stainless steel

    SciTech Connect (OSTI)

    Schnee, R. W.; Bowles, M. A.; Bunker, R.; McCabe, K.; White, J. [Department of Physics, Syracuse University, Syracuse, NY 13244 (United States)] [Department of Physics, Syracuse University, Syracuse, NY 13244 (United States); Cushman, P.; Pepin, M. [School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455 (United States)] [School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455 (United States); Guiseppe, V. E. [University of South Dakota, Vermillion, South Dakota 57069 (United States)] [University of South Dakota, Vermillion, South Dakota 57069 (United States)

    2013-08-08

    Long-lived alpha and beta emitters in the {sup 222}Rn decay chain on detector surfaces may be the limiting background in many experiments attempting to detect dark matter or neutrinoless double beta decay. Removal of tens of microns of material via electropolishing has been shown to be effective at removing radon daughters implanted into material surfaces. Some applications, however, require the removal of uniform and significantly smaller thicknesses. Here, we demonstrate that electropolishing < 1 ?m from stainless-steel plates reduces the contamination efficiently, by a factor > 100. Examination of electropolished wires with a scanning electron microscope confirms that the thickness removed is reproducible and reasonably uniform. Together, these tests demonstrate the effectiveness of removal of radon daughters for a proposed low-radiation, multi-wire proportional chamber (the BetaCage), without compromising the screeners energy resolution. More generally, electropolishing thin layers of stainless steel may effectively remove radon daughters without compromising precision-machined parts.

  15. Enhanced-locality fiber-optic two-photon-fluorescence live-brain interrogation

    SciTech Connect (OSTI)

    Fedotov, I. V.; Doronina-Amitonova, L. V.; Sidorov-Biryukov, D. A.; Fedotov, A. B.; Anokhin, K. V.; Kilin, S. Ya.; Sakoda, K.; Zheltikov, A. M.

    2014-02-24

    Two-photon excitation is shown to substantially enhance the locality of fiber-based optical interrogation of strongly scattering biotissues. In our experiments, a high-numerical-aperture, large-core-are fiber probe is used to deliver the 200-fs output of a 100-MHz mode-locked ytterbium fiber laser to samples of live mouse brain, induce two-photon fluorescence of nitrogen–vacancy centers in diamond markers in brain sample. Fiber probes with a high numerical aperture and a large core area are shown to enable locality enhancement in fiber-laser–fiber-probe two-photon brain excitation and interrogation without sacrificing the efficiency of fluorescence response collection.

  16. Technology Solutions Case Study: Air Leakage and Air Transfer Between Garage and Living Space, Waldorf, Maryland

    SciTech Connect (OSTI)

    2014-11-01

    In this project, Building Science Corporation worked with production homebuilder K. Hovnanian to evaluate air transfer between the garage and living space in a single-family detached home constructed by a production homebuilder in compliance with the 2009 International Residential Code and the 2009 International Energy Conservation Code. The project gathered important information about the performance of whole-building ventilation systems and garage ventilation systems as they relate to minimizing flow of contaminated air from garage to living space. A series of 25 multipoint fan pressurization tests and additional zone pressure diagnostic testing measured the garage and house air leakage, the garage-to-house air leakage, and garage and house pressure relationships to each other and to outdoors using automated fan pressurization and pressure monitoring techniques. While the relative characteristics of this house may not represent the entire population of new construction configurations and air tightness levels (house and garage) throughout the country, the technical approach was conservative and should reasonably extend the usefulness of the results to a large spectrum of house configurations from this set of parametric tests in this one house. Based on the results of this testing, the two-step garage-to-house air leakage test protocol described above is recommended where whole-house exhaust ventilation is employed. For houses employing whole-house supply ventilation (positive pressure) or balanced ventilation (same pressure effect as the baseline condition), adherence to the EPA Indoor airPLUS house-to-garage air sealing requirements should be sufficient to expect little to no garage-to-house air transfer.

  17. Biomass growth restriction in a packed bed reactor

    DOE Patents [OSTI]

    Griffith, William L.; Compere, Alicia L.

    1978-01-01

    When carrying out continuous biologically catalyzed reactions with anaerobic microorganisms attached to a support in an upflow packed bed column, growth of the microorganisms is restricted to prevent the microorganisms from plugging the column by limiting the availability of an essential nutrient and/or by the presence of predatory protozoa which consume the anaerobic microorganisms. A membrane disruptive detergent may be provided in the column to lyse dead microorganisms to make them available as nutrients for live microorganisms.

  18. Realistic fission models, new beta-decay half-lives and the r-process in neutron star mergers

    SciTech Connect (OSTI)

    Shibagaki, S.; Kajino, T. [Department of Astronomy, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan and National Astronomical Observatory of Japan, 2-21-1 Osawa, Mitaka, Tokyo 181-8588 (Japan); Chiba, S. [Research Laboratory for Nuclear Reactors, Tokyo Institute of Technology, Ookayama, Meguro-ku, Tokyo, 152-8850 (Japan); Lorusso, G.; Nishimura, S. [RIKEN Nishina Center, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan); Mathews, G. J. [Center for Astrophysics, Department of Physics, University of Notre Dame, IN 46556 (United States)

    2014-05-02

    Almost half of heavy nuclei beyond iron are considered to be produced by rapid neutron capture process (r-process). This process occurs in the neutron-rich environment such as core-collapse supernovae or neutron star mergers, but the main production site is still unknown. In the r-process of neutron star mergers, nuclear fission reactions play an important role. Also beta-decay half-lives of magic nuclei are crucial for the r-process. We have carried out r-process nucleosynthesis calculations based upon new theoretical estimates of fission fragment distributions and new beta-decay half-lives for N=82 nuclei measured at RIBF-RIKEN. We investigate the effect of nuclear fission on abundance patterns in the matter ejected from neutron star mergers with two different fission fragment mass distributions. We also discuss how the new experimental beta-decay half-lives affect the r-process.

  19. "Table HC10.2 Living Space Characteristics by U.S. Census Region, 2005"

    U.S. Energy Information Administration (EIA) Indexed Site

    2 Living Space Characteristics by U.S. Census Region, 2005" " Million U.S. Housing Units" ,"Housing Units (millions)","U.S. Census Region" "Living Space Characteristics",,"Northeast","Midwest","South","West" "Total",111.1,20.6,25.6,40.7,24.2 "Floorspace (Square Feet)" "Total Floorspace1" "Fewer than 500",3.2,0.9,0.5,0.9,1 "500 to 999",23.8,4.6,3.9,9,6.3

  20. "Table HC15.2 Living Space Characteristics by Four Most Populated States, 2005"

    U.S. Energy Information Administration (EIA) Indexed Site

    2 Living Space Characteristics by Four Most Populated States, 2005" " Million U.S. Housing Units" ,"U.S. Housing Units (millions)","Four Most Populated States" "Living Space Characteristics",,"New York","Florida","Texas","California" "Total",111.1,7.1,7,8,12.1 "Floorspace (Square Feet)" "Total Floorspace1" "Fewer than 500",3.2,0.4,"Q","Q",0.5 "500

  1. "Table HC3.2 Living Space Characteristics by Owner-Occupied Housing Units, 2005"

    U.S. Energy Information Administration (EIA) Indexed Site

    2 Living Space Characteristics by Owner-Occupied Housing Units, 2005" " Million U.S. Housing Units" ,," Owner-Occupied Housing Units (millions)","Type of Owner-Occupied Housing Unit" ," Housing Units (millions) " ,,,"Single-Family Units",,"Apartments in Buildings With--" "Living Space Characteristics",,,"Detached","Attached","2 to 4 Units","5 or More Units","Mobile Homes"

  2. "Table HC4.2 Living Space Characteristics by Renter-Occupied Housing Units, 2005"

    U.S. Energy Information Administration (EIA) Indexed Site

    2 Living Space Characteristics by Renter-Occupied Housing Units, 2005" " Million U.S. Housing Units" ,," Renter-Occupied Housing Units (millions)","Type of Renter-Occupied Housing Unit" ,"U.S. Housing Units (millions" ,,,"Single-Family Units",,"Apartments in Buildings With--" "Living Space Characteristics",,,"Detached","Attached","2 to 4 Units","5 or More Units","Mobile

  3. Phase Preference by Active, Acetate-Utilizing Bacteria at the Rifle, CO Integrated Field Research Challenge Site

    SciTech Connect (OSTI)

    Kerkhof, L.; Williams, K.H.; Long, P.E.; McGuinness, L.

    2011-02-21

    Previous experiments at the Rifle, Colorado Integrated Field Research Challenge (IFRC) site demonstrated that field-scale addition of acetate to groundwater reduced the ambient soluble uranium concentration. In this report, sediment samples collected before and after acetate field addition were used to assess the active microbes via {sup 13}C acetate stable isotope probing on 3 phases [coarse sand, fines (8-approximately 150 {micro}m), groundwater (0.2-8 {micro}m)] over a 24-day time frame. TRFLP results generally indicated a stronger signal in {sup 13}C-DNA in the 'fines' fraction compared to the sand and groundwater. Before the field-scale acetate addition, a Geobacter-like group primarily synthesized {sup 13}C-DNA in the groundwater phase, an alpha Proteobacterium primarily grew on the fines/sands, and an Acinetobacter sp. and Decholoromonas-like OTU utilized much of the {sup 13}C acetate in both groundwater and particle-associated phases. At the termination of the field-scale acetate addition, the Geobacter-like species was active on the solid phases rather than the groundwater, while the other bacterial groups had very reduced newly synthesized DNA signal. These findings will help to delineate the acetate utilization patterns of bacteria in the field and can lead to improved methods for stimulating distinct microbial populations in situ.

  4. Efficient Analysis of Live and Historical Streaming Data and itsApplication to Cybersecurity

    SciTech Connect (OSTI)

    Reiss, Frederick; Stockinger, Kurt; Wu, Kesheng; Shoshani, Arie; Hellerstein, Joseph M.

    2007-04-06

    Applications that query data streams in order to identifytrends, patterns, or anomalies can often benefit from comparing the livestream data with archived historical stream data. However, searching thishistorical data in real time has been considered so far to beprohibitively expensive. One of the main bottlenecks is the update costsof the indices over the archived data. In this paper, we address thisproblem by using our highly-efficient bitmap indexing technology (calledFastBit) and demonstrate that the index update operations aresufficiently efficient for this bottleneck to be removed. We describe ourprototype system based on the TelegraphCQ streaming query processor andthe FastBit bitmap index. We present a detailed performance evaluation ofour system using a complex query workload for analyzing real networktraffic data. The combined system uses TelegraphCQ to analyze streams oftraffic information and FastBit to correlate current behaviors withhistorical trends. We demonstrate that our system can simultaneouslyanalyze (1) live streams with high data rates and (2) a large repositoryof historical stream data.

  5. SEALING LARGE-DIAMETER CAST-IRON PIPE JOINTS UNDER LIVE CONDITIONS

    SciTech Connect (OSTI)

    Kiran M. Kothari; Gerard T. Pittard

    2005-07-01

    Utilities in the U.S. operate over 75,000 km (47,000 miles) of old cast-iron pipes for gas distribution. Bell-and-spigot joints that connect pipe sections together tend to leak as these pipes age. Current repair practices are costly and highly disruptive. The objective of this program is to design, test and commercialize a robotic system capable of sealing multiple castiron bell and spigot joints from a single pipe entry point. The proposed system will perform repairs with the pipe in service by traveling through the pipe, cleaning each joint surface, and installing a stainless-steel sleeve lined with an epoxy-impregnated felt across the joint. This approach will save considerable time and labor, minimize excavation, avoid traffic disruption, and eliminate any requirement to interrupt service to customers (which would result in enormous expense to utilities). Technical challenges include: (1) repair sleeves must compensate for diametric variation and eccentricity of old cast-iron pipes; (2) the assembly must travel long distances through pipes containing debris; (3) the pipe wall must be effectively cleaned in the immediate area of the joint to assure good bonding of the sleeve; and (4) an innovative bolt-on entry fitting is required to conduct safe repair operations on live mains.

  6. Search for long-lived particles in e+e- collisions

    SciTech Connect (OSTI)

    Lees, J. P.

    2015-04-29

    In this study, we present a search for a neutral, long-lived particle L that is produced in e+e collisions and decays at a significant distance from the e+e interaction point into various flavor combinations of two oppositely charged tracks. The analysis uses an e+e data sample with a luminosity of 489.1 fb1 collected by the BABAR detector at the ?(4S), ?(3S), and ?(2S) resonances and just below the ?(4S). Fitting the two-track mass distribution in search of a signal peak, we do not observe a significant signal, and set 90% confidence level upper limits on the product of the L production cross section, branching fraction, and reconstruction efficiency for six possible two-body L decay modes as a function of the L mass. The efficiency is given for each final state as a function of the mass, lifetime, and transverse momentum of the candidate, allowing application of the upper limits to any production model. In addition, upper limits are provided on the branching fraction B(B ? XsL), where Xs is a strange hadronic system.

  7. Measurement of the body composition of living gray seals by hydrogen isotope dilution

    SciTech Connect (OSTI)

    Reilly, J.J.; Fedak, M.A. )

    1990-09-01

    The body composition of living gray seals (Halichoerus grypus) can be accurately predicted from a two-step model that involves measurement of total body water (TBW) by {sup 2}H or {sup 3}H dilution and application of predictive relationships between body components and TBW that were derived empirically by slaughter chemical analysis. TBW was overestimated by both {sup 2}HHO and {sup 3}HHO dilution; mean overestimates were 2.8 +/- 0.9% (SE) with 2H and 4.0 +/- 0.6% with {sup 3}H. The relationships for prediction of total body fat (TBF), protein (TBP), gross energy (TBGE), and ash (TBA) were as follows: %TBF = 105.1 - 1.47 (%TBW); %TBP = 0.42 (%TBW) - 4.75; TBGE (MJ) = 40.8 (mass in kg) - 48.5 (TBW in kg) - 0.4; and TBA (kg) = 0.1 - 0.008 (mass in kg) + 0.05 (TBW in kg). These relationships are applicable to gray seals of both sexes over a wide range of age and body conditions, and they predict the body composition of gray seals more accurately than the predictive equations derived from ringed seals (Pusa hispida) and from the equation of Pace and Rathbun, which has been reported to be generally applicable to mammals.

  8. In situ capping for size control of monochalcogenides (ZnS, CdS, and SnS) nanocrystals produced by anaerobic metal-reducing bacteria

    SciTech Connect (OSTI)

    Jang, Gyoung Gug; Jacobs, Christopher B; Ivanov, Ilia N; Joshi, Pooran C; Meyer III, Harry M; Kidder, Michelle; Armstrong, Beth L; Datskos, Panos G; Graham, David E; Moon, Ji Won

    2015-01-01

    Metal monochalcogenide quantum dot nanocrystals of ZnS, CdS and SnS were prepared by anaerobic, metal-reducing bacteria using in situ capping by oleic acid or oleylamine. The capping agent preferentially adsorbs on the surface of the nanocrystal, suppressing the growth process in the early stages, thus leading to production of nanocrystals with a diameter of less than 5 nm.

  9. Search for Charged Massive Long-Lived Particles Using the D0 Detector

    SciTech Connect (OSTI)

    Xie, Yunhe; /Brown U.

    2009-05-01

    A search for charged massive stable particles has been performed with the D0 detector using 1.1 fb{sup -1} of data. The speed of the particle has been calculated based on the time-of-flight and position information in the muon system. The present research is limited to direct pair-production of the charged massive long-lived particles. We do not consider CMSPs that result from the cascade decays of heavier particles. In this analysis, the exact values of the model parameters of the entire supersymmetric particle mass spectrum, relevant for cascade decays, are not important. We found no evidence of the signal. 95% CL cross-section upper limits have been set on the pair-productions of the stable scaler tau lepton, the gaugino-like charginos, and the higgsino-like charginos. The upper cross section limits vary from 0.31 pb to 0.04 pb, for stau masses in the range between 60 GeV and 300 GeV. We use the nominal value of the theoretical cross section to set limits on the mass of the pair produced charginos. We exclude the pair-produced stable gaugino-like charginos with mass below 206 GeV, and higgsino-like charginos below 171 GeV, respectively. Although the present sensitivity is insufficient to test the model of the pair produced stable staus, we do set cross section limits which can be applied to the pair production of any charged massive stable particle candidates with similar kinematics. These are the most restrictive limits to the present on the cross sections for CMSPs and the first published from the Tevatron Collider Run II. The manuscript has been published by Physical Review Letters in April 2009 and is available at arXiv as.

  10. The fate of long-lived superparticles with hadronic decays after LHC Run 1

    SciTech Connect (OSTI)

    Liu, Zhen; Tweedie, Brock

    2015-06-08

    Supersymmetry searches at the LHC are both highly varied and highly constraining, but the vast majority are focused on cases where the final-stage visible decays are prompt. Scenarios featuring superparticles with detector-scale lifetimes have therefore remained a tantalizing possibility for sub-TeV SUSY, since explicit limits are relatively sparse. Nonetheless, the extremely low backgrounds of the few existing searches for collider-stable and displaced new particles facilitates recastings into powerful long-lived superparticle searches, even for models for which those searches are highly non-optimized. In this paper, we assess the status of such models in the context of baryonic R-parity violation, gauge mediation, and mini-split SUSY. We explore a number of common simplified spectra where hadronic decays can be important, employing recasts of LHC searches that utilize different detector systems and final-state objects. The LSP/NLSP possibilities considered here include generic colored superparticles such as the gluino and light-flavor squarks, as well as the lighter stop and the quasi-degenerate Higgsino multiplet motivated by naturalness. We find that complementary coverage over large swaths of mass and lifetime is achievable by superimposing limits, particularly from CMSs tracker-based displaced dijet search and heavy stable charged particle searches. Adding in prompt searches, we find many cases where a range of sparticle masses is now excluded from zero lifetime to infinite lifetime with no gaps. In other cases, the displaced searches furnish the only extant limits at any lifetime.

  11. The fate of long-lived superparticles with hadronic decays after LHC Run 1

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Liu, Zhen; Tweedie, Brock

    2015-06-08

    Supersymmetry searches at the LHC are both highly varied and highly constraining, but the vast majority are focused on cases where the final-stage visible decays are prompt. Scenarios featuring superparticles with detector-scale lifetimes have therefore remained a tantalizing possibility for sub-TeV SUSY, since explicit limits are relatively sparse. Nonetheless, the extremely low backgrounds of the few existing searches for collider-stable and displaced new particles facilitates recastings into powerful long-lived superparticle searches, even for models for which those searches are highly non-optimized. In this paper, we assess the status of such models in the context of baryonic R-parity violation, gauge mediation,more »and mini-split SUSY. We then explore a number of common simplified spectra where hadronic decays can be important, employing recasts of LHC searches that utilize different detector systems and final-state objects. The LSP/NLSP possibilities considered here include generic colored superparticles such as the gluino and light-flavor squarks, as well as the lighter stop and the quasi-degenerate Higgsino multiplet motivated by naturalness. We find that complementary coverage over large swaths of mass and lifetime is achievable by superimposing limits, particularly from CMS’s tracker-based displaced dijet search and heavy stable charged particle searches. By adding in prompt searches, we find many cases where a range of sparticle masses is now excluded from zero lifetime to infinite lifetime with no gaps. In other cases, the displaced searches furnish the only extant limits at any lifetime.« less

  12. MIXING AND TRANSPORT OF SHORT-LIVED AND STABLE ISOTOPES AND REFRACTORY GRAINS IN PROTOPLANETARY DISKS

    SciTech Connect (OSTI)

    Boss, Alan P.

    2013-08-10

    Analyses of primitive meteorites and cometary samples have shown that the solar nebula must have experienced a phase of large-scale outward transport of small refractory grains as well as homogenization of initially spatially heterogeneous short-lived isotopes. The stable oxygen isotopes, however, were able to remain spatially heterogeneous at the {approx}6% level. One promising mechanism for achieving these disparate goals is the mixing and transport associated with a marginally gravitationally unstable (MGU) disk, a likely cause of FU Orionis events in young low-mass stars. Several new sets of MGU models are presented that explore mixing and transport in disks with varied masses (0.016 to 0.13 M{sub Sun }) around stars with varied masses (0.1 to 1 M{sub Sun }) and varied initial Q stability minima (1.8 to 3.1). The results show that MGU disks are able to rapidly (within {approx}10{sup 4} yr) achieve large-scale transport and homogenization of initially spatially heterogeneous distributions of disk grains or gas. In addition, the models show that while single-shot injection heterogeneity is reduced to a relatively low level ({approx}1%), as required for early solar system chronometry, continuous injection of the sort associated with the generation of stable oxygen isotope fractionations by UV photolysis leads to a sustained, relatively high level ({approx}10%) of heterogeneity, in agreement with the oxygen isotope data. These models support the suggestion that the protosun may have experienced at least one FU Orionis-like outburst, which produced several of the signatures left behind in primitive chondrites and comets.

  13. The fate of long-lived superparticles with hadronic decays after LHC Run 1

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Liu, Zhen; Tweedie, Brock

    2015-06-08

    Supersymmetry searches at the LHC are both highly varied and highly constraining, but the vast majority are focused on cases where the final-stage visible decays are prompt. Scenarios featuring superparticles with detector-scale lifetimes have therefore remained a tantalizing possibility for sub-TeV SUSY, since explicit limits are relatively sparse. Nonetheless, the extremely low backgrounds of the few existing searches for collider-stable and displaced new particles facilitates recastings into powerful long-lived superparticle searches, even for models for which those searches are highly non-optimized. In this paper, we assess the status of such models in the context of baryonic R-parity violation, gauge mediation,moreand mini-split SUSY. We explore a number of common simplified spectra where hadronic decays can be important, employing recasts of LHC searches that utilize different detector systems and final-state objects. The LSP/NLSP possibilities considered here include generic colored superparticles such as the gluino and light-flavor squarks, as well as the lighter stop and the quasi-degenerate Higgsino multiplet motivated by naturalness. We find that complementary coverage over large swaths of mass and lifetime is achievable by superimposing limits, particularly from CMSs tracker-based displaced dijet search and heavy stable charged particle searches. Adding in prompt searches, we find many cases where a range of sparticle masses is now excluded from zero lifetime to infinite lifetime with no gaps. In other cases, the displaced searches furnish the only extant limits at any lifetime.less

  14. Wireless Self-powered Visual and NDE Robotic Inspection System for Live Gas Distribution Mains

    SciTech Connect (OSTI)

    Susan Burkett; Hagen Schempf

    2006-01-31

    Carnegie Mellon University (CMU) under contract from Department of Energy/National Energy Technology Laboratory (DoE/NETL) and co-funding from the Northeast Gas Association (NGA), has completed the overall system design of the next-generation Explorer-II (X-II) live gas main NDE and visual inspection robot platform. The design is based on the Explorer-I prototype which was built and field-tested under a prior (also DoE- and NGA co-funded) program, and served as the validation that self-powered robots under wireless control could access and navigate live natural gas distribution mains. The X-II system design ({approx}8 ft. and 66 lbs.) was heavily based on the X-I design, yet was substantially expanded to allow the addition of NDE sensor systems (while retaining its visual inspection capability), making it a modular system, and expanding its ability to operate at pressures up to 750 psig (high-pressure and unpiggable steel-pipe distribution mains). A new electronics architecture and on-board software kernel were added to again improve system performance. A locating sonde system was integrated to allow for absolute position-referencing during inspection (coupled with external differential GPS) and emergency-locating. The power system was upgraded to utilize lithium-based battery-cells for an increase in mission-time. The system architecture now relies on a dual set of end camera-modules to house the 32-bit processors (Single-Board Computer or SBC) as well as the imaging and wireless (off-board) and CAN-based (on-board) communication hardware and software systems (as well as the sonde-coil and -electronics). The drive-module (2 ea.) are still responsible for bracing (and centering) to drive in push/pull fashion the robot train into and through the pipes and obstacles. The steering modules and their arrangement, still allow the robot to configure itself to perform any-angle (up to 90 deg) turns in any orientation (incl. vertical), and enable the live launching and recovery of the system using custom fittings and a (to be developed) launch-chamber/-tube. The battery modules are used to power the system, by providing power to the robot's bus. The support modules perform the functions of centration for the rest of the train as well as odometry pickups using incremental encoding schemes. The electronics architecture is based on a distributed (8-bit) microprocessor architecture (at least 1 in ea. module) communicating to a (one of two) 32-bit SBC, which manages all video-processing, posture and motion control as well as CAN and wireless communications. The operator controls the entire system from an off-board (laptop) controller, which is in constant wireless communication with the robot train in the pipe. The sensor modules collect data and forward it to the robot operator computer (via the CAN-wireless communications chain), who then transfers it to a dedicated NDE data-storage and post-processing computer for further (real-time or off-line) analysis. CMU has fully designed every module in terms of the mechanical, electrical and software elements (architecture only). Substantial effort has gone into pre-prototyping to uncover mechanical, electrical and software issues for critical elements of the design. Design requirements for sensor-providers were also detailed and finalized and provided to them for inclusion in their designs. CMU is expecting to start 2006 with a detailed design effort for both mechanical and electrical components, followed by procurement and fabrication efforts in late winter/spring 2006. The assembly and integration efforts will occupy all of the spring and summer of 2006. Software development will also be a major effort in 2006, and will result in porting and debugging of code on the module- and train-levels in late summer and Fall of 2006. Final pipe mock-up testing is expected in late fall and early winter 2006 with an acceptance demonstration of the robot train (with a sensor-module mock-up) planned to DoE/NGA towards the end of 2006.

  15. First Use of High Charge States for Mass Measurements of Short-Lived Nuclides in a Penning Trap

    SciTech Connect (OSTI)

    Ettenauer, S.; Gallant, A. T.; Dilling, J.; Simon, M. C.; Chaudhuri, A.; Mane, E.; Delheij, P.; Pearson, M. R.; Brunner, T.; Chowdhury, U.; Simon, V. V.; Brodeur, M.; Andreoiu, C.; Audi, G.; Lopez-Urrutia, J. R. Crespo; Ullrich, J.; Gwinner, G.; Lapierre, A.; Lunney, D.; Ringle, R.

    2011-12-30

    Penning trap mass measurements of short-lived nuclides have been performed for the first time with highly charged ions, using the TITAN facility at TRIUMF. Compared to singly charged ions, this provides an improvement in experimental precision that scales with the charge state q. Neutron-deficient Rb isotopes have been charge bred in an electron beam ion trap to q=8-12+ prior to injection into the Penning trap. In combination with the Ramsey excitation scheme, this unique setup creating low energy, highly charged ions at a radioactive beam facility opens the door to unrivaled precision with gains of 1-2 orders of magnitude. The method is particularly suited for short-lived nuclides such as the superallowed {beta} emitter {sup 74}Rb (T{sub 1/2}=65 ms). The determination of its atomic mass and an improved Q{sub EC} value are presented.

  16. Long-lived charge carrier generation in ordered films of a covalent perylenediimide–diketopyrrolopyrrole–perylenediimide molecule

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hartnett, Patrick E.; Dyar, Scott M.; Margulies, Eric A.; Shoer, Leah E.; Cook, Andrew W.; Eaton, Samuel W.; Marks, Tobin J.; Wasielewski, Michael R.

    2015-07-31

    The photophysics of a covalently linked perylenediimide–diketopyrrolopyrrole–perylenediimide acceptor–donor–acceptor molecule (PDI–DPP–PDI, 1) were investigated and found to be markedly different in solution versus in unannealed and solvent annealed films. Photoexcitation of 1 in toluene results in quantitative charge separation in τ = 3.1 ± 0.2 ps, with charge recombination in τ = 340 ± 10 ps, while in unannealed/disordered films of 1, charge separation occurs in τ < 250 fs, while charge recombination displays a multiexponential decay in ~6 ns. The absence of long-lived, charge separation in the disordered film suggests that few free charge carriers are generated. In contrast, uponmore » CH₂Cl₂ vapor annealing films of 1, grazing-incidence X-ray scattering shows that the molecules form a more ordered structure. Photoexcitation of the ordered films results in initial formation of a spin-correlated radical ion pair (electron–hole pair) as indicated by magnetic field effects on the formation of free charge carriers which live for ~4 μs. This result has significant implications for the design of organic solar cells based on covalent donor–acceptor systems and shows that long-lived, charge-separated states can be achieved by controlling intramolecular charge separation dynamics in well-ordered systems.« less

  17. Long-lived charge carrier generation in ordered films of a covalent perylenediimidediketopyrrolopyrroleperylenediimide molecule

    SciTech Connect (OSTI)

    Hartnett, Patrick E.; Dyar, Scott M.; Margulies, Eric A.; Shoer, Leah E.; Cook, Andrew W.; Eaton, Samuel W.; Marks, Tobin J.; Wasielewski, Michael R.

    2015-07-31

    The photophysics of a covalently linked perylenediimidediketopyrrolopyrroleperylenediimide acceptordonoracceptor molecule (PDIDPPPDI, 1) were investigated and found to be markedly different in solution versus in unannealed and solvent annealed films. Photoexcitation of 1 in toluene results in quantitative charge separation in ? = 3.1 0.2 ps, with charge recombination in ? = 340 10 ps, while in unannealed/disordered films of 1, charge separation occurs in ? < 250 fs, while charge recombination displays a multiexponential decay in ~6 ns. The absence of long-lived, charge separation in the disordered film suggests that few free charge carriers are generated. In contrast, upon CH?Cl? vapor annealing films of 1, grazing-incidence X-ray scattering shows that the molecules form a more ordered structure. Photoexcitation of the ordered films results in initial formation of a spin-correlated radical ion pair (electronhole pair) as indicated by magnetic field effects on the formation of free charge carriers which live for ~4 ?s. This result has significant implications for the design of organic solar cells based on covalent donoracceptor systems and shows that long-lived, charge-separated states can be achieved by controlling intramolecular charge separation dynamics in well-ordered systems.

  18. SEALING LARGE-DIAMETER CAST-IRON PIPE JOINTS UNDER LIVE CONDITIONS

    SciTech Connect (OSTI)

    Kiran M. Kothari; Gerard T. Pittard

    2003-01-01

    Utilities in the U.S. operate over 75,000 km (47,000 miles) of old cast-iron pipes for gas distribution. The bell-and-spigot joints tend to leak as these pipes age. Current repair practices are costly and highly disruptive. The objective of this program is to design, test and commercialize a robotic system capable of sealing multiple cast-iron bell and spigot joints from a single pipe entry point. The proposed system will perform repairs while the pipe remains in service by traveling through the pipe, cleaning each joint surface, and attaching a stainless-steel sleeve lined with an epoxy-impregnated felt across the joint. This approach will save considerable time and labor, avoid traffic disruption, and eliminate any requirement to interrupt service (which results in enormous expense to utilities). Technical challenges include: (1) repair sleeves must compensate for diametric variation and eccentricity of cast-iron pipes; (2) the assembly must travel long distances through pipes containing debris; (3) the pipe wall must be effectively cleaned in the immediate area of the joint to assure good bonding of the sleeve; and (4) an innovative bolt-on entry fitting is required to conduct repair operations on live mains. The development effort is divided into eleven tasks. Task 1--Program Management was previously completed. Two reports, one describing the program management plan and the other consisting of the technology assessment, were submitted to the DOE COR in the first quarter. Task 2--Establishment of Detailed Design Specifications and Task 3--Design and Fabricate Ratcheting Stainless-Steel Repair Sleeves are now well underway. First-quarter activities included conducting detailed analyses to determine the capabilities of coiled-tubing locomotion for entering and repairing gas mains and the first design iteration of the joint-sealing sleeve. The maximum horizontal reach of coiled tubing inside a pipeline before buckling prevents further access was calculated for a wide range of coiled-tubing string designs and pipe environments. Work conducted in the second quarter consisted of: (1) selecting a preferred pan/zoom/tilt camera; (2) initiating design of the digital control electronics and switching power supply for the control and operation of the in-pipe robotic modules; (3) continuing design of the repair sleeve and (4) initial testing of the wall-cleaning device. Most recently, activities in the third quarter included: (1) development of the system's pan/zoom/tilt camera control electronics and operating software, and implementing these in the surface and downhole modules and (2) further testing of the wall-cleaning elements used to clean the inside of the bell and spigot joints. Details of these activities are described in the body of the report along with a summary of events scheduled for the fourth quarter.

  19. SEALING LARGE-DIAMETER CAST-IRON PIPE JOINTS UNDER LIVE CONDITIONS

    SciTech Connect (OSTI)

    Kiran M. Kothari; Gerard T. Pittard

    2003-06-01

    Utilities in the U.S. operate over 75,000 km (47,000 miles) of old cast-iron pipes for gas distribution. The bell-and-spigot joints tend to leak as these pipes age. Current repair practices are costly and highly disruptive. The objective of this program is to design, test and commercialize a robotic system capable of sealing multiple cast-iron bell and spigot joints from a single pipe entry point. The proposed system will perform repairs while the pipe remains in service by traveling through the pipe, cleaning each joint surface, and attaching a stainless-steel sleeve lined with an epoxy-impregnated felt across the joint. This approach will save considerable time and labor, avoid traffic disruption, and eliminate any requirement to interrupt service (which results in enormous expense to utilities). Technical challenges include: (1) repair sleeves must compensate for diametric variation and eccentricity of cast-iron pipes; (2) the assembly must travel long distances through pipes containing debris; (3) the pipe wall must be effectively cleaned in the immediate area of the joint to assure good bonding of the sleeve; and (4) an innovative bolt-on entry fitting is required to conduct repair operations on live mains. The development effort is divided into eleven tasks. Task 1-Program Management was previously completed. Two reports, one describing the program management plan and the other consisting of the technology assessment, were submitted to the DOE COR in the first quarter. Task 2-Establishment of Detailed Design Specifications and Task 3-Design and Fabricate Ratcheting Stainless-Steel Repair Sleeves are now well underway. First-quarter activities included conducting detailed analyses to determine the capabilities of coiled-tubing locomotion for entering and repairing gas mains and the first design iteration of the joint-sealing sleeve. The maximum horizontal reach of coiled tubing inside a pipeline before buckling prevents further access was calculated for a wide range of coiled-tubing string designs and pipe environments. Work conducted in the second quarter consisted of: (1) selecting a preferred pan/zoom/tilt camera; (2) initiating design of the digital control electronics and switching power supply for the control and operation of the in-pipe robotic modules; (3) continuing design of the repair sleeve and (4) initial testing of the wall-cleaning device. Activities in the third quarter included: (1) development of the system's pan/zoom/tilt camera control electronics and operating software, and implementing these in the surface and downhole modules and (2) further testing of the wall-cleaning elements used to clean the inside of the bell and spigot joints. Most recently, fourth quarter developments were centered on designing and testing the pipe-wall cleaning device including the selection of the drive motor and its control electronics. In addition, efforts were also focused on the design of the repair sleeve. Details of these activities are described in the body of the report along with a summary of events scheduled for the next quarter.

  20. Geological Repository Layout for Radioactive High Level Long Lived Waste in Argilite

    SciTech Connect (OSTI)

    Gaussen, J.L.

    2006-07-01

    In the framework of the 1991 French radioactive waste act, ANDRA has studied the feasibility of a geological repository in the argillite layer of the Bure site for high-level long-lived waste. This presentation is focused on the underground facilities that constitute the specific component of this project. The preliminary underground layout, which has been elaborated, is based on four categories of data: - the waste characteristics and inventory; - the geological properties of the host argillite; - the long term performance objectives of the repository; - the specifications in term of operation and reversibility. The underground facilities consist of two types of works: the access works (shafts and drifts) and the disposal cells. The function of the access works is to permit the implementation of two concurrent activities: the nuclear operations (transfer and emplacement of the disposal packages into the disposal cells) and the construction of the next disposal cells. The design of the drifts network which matches up to this function is also influenced by two other specifications: the minimisation of the drift dimensions in order to limit their influence on the integrity of the geological formation and the necessity of a safe ventilation in case of fire. The resulting layout is a network of 4 parallel drifts (2 of them being dedicated to the operation, the other two being dedicated to the construction activities). The average diameter of these access drifts is 7 meters. 4 shafts ensure the link between the surface and the underground. The most important function of the disposal cells is to contribute to the long-term performance of the repository. In this regard, the thermal and geotechnical considerations play an important role. The B wastes (intermediate level wastes) are not (or not very) exothermic. Consequently, the design of their disposal cells result mainly from geotechnical considerations. The disposal packages (made of concrete) are piled up in big cavities the diameter of which is about 10 meters and the length of which is about 250 meters. On the other hand, the design of the C waste disposal cells (vitrified waste) is mainly derived from their thermal power (about 500 W after a 60 year period of interim storage). The disposal cell is a tunnel the diameter of which is about 0,70 m and the length of which is about 40 m. The number of the disposal packages (made of steel) per cell, the spacing between two adjacent canisters within a given cell and the spacing between two adjacent cells are adjusted to limit the peak of temperature in the host formation at 100 deg. C. The disposal cells are also characterized by favourable design factors that would facilitate the potential retrieval of the wastes. The whole underground layout would represent a surface area of several km{sup 2}. (authors)

  1. Comparison of killing of gram-negative and gram-positive bacteria by pure singlet oxygen. [Salmonella typhimurium; Escherichia coli; Sarcina lutea; Staphylococcus aureus; Streptococcus lactis; Streptococcus faecalis

    SciTech Connect (OSTI)

    Dahl, T.A.; Midden, W.R. ); Hartman, P.E. )

    1989-04-01

    Gram-negative and gram-positive bacteria were found to display different sensitivities to pure singlet oxygen generated outside of cells. Killing curves for Salmonella typhimurium and Escherichia coli strains were indicative of multihit killing, whereas curves for Sarcina lutea, Staphylococcus aureus, Streptococcus lactis, and Streptococcus faecalis exhibited single-hit kinetics. The S. typhimurium deep rough strain TA1975, which lacks nearly all of the cell wall lipopolysaccharide coat and manifests concomitant enhancement of penetration by some exogenous substances, responded to singlet oxygen with initially faster inactivation than did the S. typhimurium wild-type strain, although the maximum rates of killing appeared to be quite similar. The structure of the cell wall thus plays an important role in susceptibility to singlet oxygen. The outer membrane-lipopolysaccharide portion of the gram-negative cell wall initially protects the bacteria from extracellular singlet oxygen, although it may also serve as a source for secondary reaction products which accentuate the rates of cell killing. S. typhimurium and E. coli strains lacking the cellular antioxidant, glutathione, showed no difference from strains containing glutathione in response to the toxic effects of singlet oxygen. Strains of Sarcina lutea and Staphylococcus aureus that contained carotenoids, however, were far more resistant to singlet oxygen lethality than were both carotenoidless mutants of the same species and other gram-positive species lacking high levels of protective carotenoids.

  2. Mathematical modeling of positron emission tomography (PET) data to assess radiofluoride transport in living plants following petiolar administration

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Converse, Alexander K.; Ahlers, Elizabeth O.; Bryan, Tom W.; Hetue, Jackson D.; Lake, Katherine A.; Ellison, Paul A.; Engle, Jonathan W.; Barnhart, Todd E.; Nickles, Robert J.; Williams, Paul H.; et al

    2015-03-15

    Background: Ion transport is a fundamental physiological process that can be studied non-invasively in living plants with radiotracer imaging methods. Fluoride is a known phytotoxic pollutant and understanding its transport in plants after leaf absorption is of interest to those in agricultural areas near industrial sources of airborne fluoride. Here we report the novel use of a commercial, high-resolution, animal positron emission tomography (PET) scanner to trace a bolus of [¹⁸F]fluoride administered via bisected petioles of Brassica oleracea, an established model species, to simulate whole plant uptake of atmospheric fluoride. This methodology allows for the first time mathematical compartmental modelingmore » of fluoride transport in the living plant. Radiotracer kinetics in the stem were described with a single-parameter free- and trapped-compartment model and mean arrival times at different stem positions were calculated from the free-compartment time-activity curves. Results: After initiation of administration at the bisected leaf stalk, [¹⁸F] radioactivity climbed for approximately 10 minutes followed by rapid washout from the stem and equilibration within leaves. Kinetic modeling of transport in the stem yielded a trapping rate of 1.5 +/- 0.3%/min (mean +/- s.d., n = 3), velocity of 2.2 +/- 1.1 cm/min, and trapping fraction of 0.8 +/- 0.5%/cm. Conclusion: Quantitative assessment of physiologically meaningful transport parameters of fluoride in living plants is possible using standard positron emission tomography in combination with petiolar radiotracer administration. Movement of free fluoride was observed to be consistent with bulk flow in xylem, namely a rapid and linear change in position with respect to time. Trapping, likely in the apoplast, was observed. Future applications of the methods described here include studies of transport of other ions and molecules of interest in plant physiology.« less

  3. Watch it Live at 1pm: Secretary Chu Talks "Sputnik Moment" at Press Club |

    Broader source: Energy.gov (indexed) [DOE]

    Department of Energy At 1pm ET today, Secretary Chu will deliver a speech at the National Press Club in DC calling on the United States to sharply accelerate innovations in clean energy -- citing China and other countries' recent advances in clean technology as a critical "Sputnik Moment" for the U.S. The event will begin at 12:30, and Chu will deliver his remarks at 1, followed by a question and answer session. You'll be able to watch live online here. UPDATE: Follow along with

  4. The role of pyrimidine and water as underlying molecular constituents for describing radiation damage in living tissue: A comparative study

    SciTech Connect (OSTI)

    Fuss, M. C.; Ellis-Gibbings, L.; Jones, D. B.; Brunger, M. J.; Blanco, F.; Muoz, A.; Limo-Vieira, P.; Garca, G.

    2015-06-07

    Water is often used as the medium for characterizing the effects of radiation on living tissue. However, in this study, charged-particle track simulations are employed to quantify the induced physicochemical and potential biological implications when a primary ionising particle with energy 10?keV strikes a medium made up entirely of water or pyrimidine. Note that pyrimidine was chosen as the DNA/RNA bases cytosine, thymine, and uracil can be considered pyrimidine derivatives. This study aims to assess the influence of the choice of medium on the charged-particle transport, and identify how appropriate it is to use water as the default medium to describe the effects of ionising radiation on living tissue. Based on the respective electron interaction cross sections, we provide a model, which allows the study of radiation effects not only in terms of energy deposition (absorbed dose and stopping power) but also in terms of the number of induced molecular processes. Results of these parameters for water and pyrimidine are presented and compared.

  5. DESIGN, CONSTRUCTION AND FIELD DEMONSTRATION OF EXPLORER: A LONG-RANGE UNTETHERED LIVE GASLINE INSPECTION ROBOT SYSTEM

    SciTech Connect (OSTI)

    Dr. George C. Vradis; Dr. Hagen Schempf

    2002-05-01

    The goal of this program is to construct and demonstrate EXPLORER, a modular, remotely controllable, self-powered, untethered robot system for the inspection of live gas distribution 150 mm (6-inch) to 200 mm (8-inch) diameter mains. The system, which was designed in an earlier effort, is built in a modular fashion in order to accommodate various components intended to accomplish different inspection, repair, sample retrieval, and other in-pipe tasks. The prototype system to be built under this project will include all the basic modules needed by the system, i.e. the locomotion, power storage, wireless communication, and camera. The camera, a solid-state fisheye-type, is used to transmit real-time video to the operator that allows for the live inspection of gas distribution pipes. This module, which incorporates technology developed by NASA, has been designed, constructed and tested in the earlier effort. In the current effort, the full prototype system will be tested in the laboratory followed by two field demonstrations in real applications in NYGAS member utilities' pipes. The purpose for EXPLORER is to be able to access live gas mains, insert the system in the piping network, and remotely ''drive'' it within the gas main and its laterals through distances of five to ten thousand feet. Its adaptable locomotion system allows the robot to function through varying diameter pipes (150 - 200 mm or 6- to 8-inches) and is powered via on-board battery-banks. The presence of fish-eye cameras in both ends of the robot allows the operator to view the forward and circumferential views of the internals live using an above-ground TV. Communication takes place via wireless link between the robot and the launch-chamber used to insert/retrieve the system. This link is based on commercial technology presently employed in wireless telecommunication networks. Communication over long distances as well as battery re-charging will be accomplished without retrieving the robot but through the use of auxiliaries, to be developed in a follow-on phase, that will allow insertion of additional antennas and battery recharge plugs into the pipe under live conditions through inexpensive keyhole sized excavations. The proposed system significantly advances the state of the art in inspection systems for gas distribution mains, which presently consist of tethered systems of limited range (about 500 ft form the point of launch) and limited inspection views. Also current inspection systems have no ability to incorporate additional modules to expand their functionality. This development program is a joint effort among the New York Gas Group (NYGAS; a trade association of the publicly owned gas utilities in New York State), the Jet Propulsion Laboratory (JPL), the Johnson Space Center (JSC), Carnegie Mellon University's (CMU) National Robotics Engineering Consortium (NREC), and the US Department of Energy (DOE) through the National Energy Technology Laboratory (NETL). The DOE's contribution to this project is $499,023 out of a total of $780,735 (not including NASA's contribution). The present report summarizes the accomplishments of the project during the first six months since funding from DOE commenced. The project has achieved its goals for this period as outlined in the report. Currently the fabrication of the prototype is in progress and it should be completed by late-summer 2002. Testing of the prototype in the lab is expected to be completed by November 2002, to be followed by two field demonstrations in early 2003.

  6. Formation of a long-lived hot field reversed configuration by dynamically merging two colliding high-{beta} compact toroids

    SciTech Connect (OSTI)

    Guo, H. Y.; Binderbauer, M. W.; Barnes, D.; Putvinski, S.; Rostoker, N.; Sevier, L.; Tuszewski, M.; Anderson, M. G.; Andow, R.; Bonelli, L.; Brown, R.; Bui, D. Q.; Bystritskii, V.; Clary, R.; Cheung, A. H.; Conroy, K. D.; Deng, B. H.; Dettrick, S. A.; Douglass, J. D.; Feng, P.

    2011-05-15

    A high temperature field reversed configuration (FRC) has been produced in the newly built, world's largest compact toroid (CT) facility, C-2, by colliding and merging two high-{beta} CTs produced using the advanced field-reversed {theta}-pinch technology. This long-lived, stable merged state exhibits the following key properties: (1) apparent increase in the poloidal flux from the first pass to the final merged state, (2) significantly improved confinement compared to conventional {theta}-pinch FRCs with flux decay rates approaching classical values in some cases, (3) strong conversion from kinetic energy into thermal energy with total temperature (T{sub e} + T{sub i}) exceeding 0.5 keV, predominantly into the ion channel. Detailed modeling using a new 2-D resistive magnetohydrodynamic (MHD) code, LamyRidge, has demonstrated, for the first time, the formation, translation, and merging/reconnection dynamics of such extremely high-{beta} plasmas.

  7. Synchrotron based infrared imaging and spectroscopy via focal plane array on live fibroblasts in D2O enriched medium

    SciTech Connect (OSTI)

    Quaroni, Luca; Zlateva, Theodora; Sarafimov, Blagoj; Kreuzer, Helen W.; Wehbe, Katia; Hegg, Eric L.; Cinque, Gianfelice

    2014-03-26

    We tested the viability of using synchrotron based infrared imaging to study biochemical processes inside living cells. As a model system, we studied fibroblast cells exposed to a medium highly enriched with D2O. We could show that the experimental technique allows us to reproduce at the cellular level measurements that are normally performed on purified biological molecules. We can obtain information about lipid conformation and distribution, kinetics of hydrogen/deuterium exchange, and the formation of concentration gradients of H and O isotopes in water that are associated with cell metabolism. The implementation of the full field technique in a sequential imaging format gives a description of cellular biochemistry and biophysics that contains both spatial and temporal information.

  8. Prevalence of respiratory symptoms and diseases in schoolchildren living in a polluted and in a low polluted area in Israel

    SciTech Connect (OSTI)

    Goren, A.I.; Hellmann, S.

    1988-02-01

    Second and fifth grade schoolchildren living in two communities with different levels of air pollution were studied. The parents of these children filled out ATS-NHLI health questionnaires. The prevalence of reported respiratory symptoms and pulmonary diseases was found to be significantly higher among children growing up in the polluted community (Ashdod) as compared with the low-pollution area (Hadera). Logistic models fitted for the respiratory conditions which differed significantly between both areas of residence also included background variables that could be responsible for these differences. Relative risk values, which were calculated from the logistic models, were in the range of 1.47 for cough without cold to 2.66 for asthma for children from Ashdod, as compared with 1.00 for children from Hadera.

  9. Building America Case Study: Air Leakage and Air Transfer Between Garage and Living Space, Waldorf, Maryland (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2014-11-01

    This research project focused on evaluation of air transfer between the garage and living space in a single-family detached home constructed by a production homebuilder in compliance with the 2009 International Residential Code and the 2009 International Energy Conservation Code. The project gathered important information about the performance of whole-building ventilation systems and garage ventilation systems as they relate to minimizing flow of contaminated air from garage to living space. A series of 25 multi-point fan pressurization tests and additional zone pressure diagnostic testing characterized the garage and house air leakage, the garage-to-house air leakage, and garage and house pressure relationships to each other and to outdoors using automated fan pressurization and pressure monitoring techniques. While the relative characteristics of this house may not represent the entire population of new construction configurations and air tightness levels (house and garage) throughout the country, the technical approach was conservative and should reasonably extend the usefulness of the results to a large spectrum of house configurations from this set of parametric tests in this one house. Based on the results of this testing, the two-step garage-to-house air leakage test protocol described above is recommended where whole-house exhaust ventilation is employed. For houses employing whole-house supply ventilation (positive pressure) or balanced ventilation (same pressure effect as the Baseline condition), adherence to the EPA Indoor airPLUS house-to-garage air sealing requirements should be sufficient to expect little to no garage-to-house air transfer.

  10. New Species of Cyanobacteria Forms Intracellular Carbonates

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    may help to weigh down the microorganisms, which live on rock substrates. Scanning electron microscopy image of two intracellularly calcifying cyanobacterial cells. Ca-, Mg-,...

  11. Search for heavy long-lived multi-charged particles in pp collisions at √s = 8  TeV using the ATLAS detector

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    None

    2015-08-08

    A search for heavy long-lived multi-charged particles is performed using the ATLAS detector at the LHC. Data collected in 2012 at √s = 8 TeV from pp collisions corresponding to an integrated luminosity of 20.3 fb-1 are examined. Particles producing anomalously high ionisation, consistent with long-lived massive particles with electric charges from |q| = 2e to |q| = 6e are searched for. No signal candidate events are observed, and 95 % confidence level cross-section upper limits are interpreted as lower mass limits for a Drell–Yan production model. The mass limits range between 660 and 785 GeV.

  12. Living in Los Alamos

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Education Center University of New Mexico - Los Alamos The Arts Fuller Lodge Art Center Los Alamos Little Theatre Reel Deal Movie Theater County Services Aquatic...

  13. Efficient Living Energy Grant

    Broader source: Energy.gov [DOE]

    Many of the Office of Energy and Recycling programs, including this one, are currently closed and are not accepting applications. When funding is available, it will be announced here and on the...

  14. Women @ Energy: Kelly Lively

    Broader source: Energy.gov [DOE]

    "Dive in. Don’t be afraid to do the work and ask questions. Initially, I was intimidated going into college, having been out for so long; but I encourage others to go back, it is never too late to learn things.When in the workforce, you have to ask questions and engage. You have to take that risk—to follow the engineering work in the field and be okay with making mistakes."

  15. RELATIVISTIC SUPERNOVAE HAVE SHORTER-LIVED CENTRAL ENGINES OR MORE EXTENDED PROGENITORS: THE CASE OF SN2012ap

    SciTech Connect (OSTI)

    Margutti, R.; Milisavljevic, D.; Soderberg, A. M.; Sanders, N.; Chakraborti, S.; Kamble, A.; Drout, M.; Parrent, J.; Zauderer, A.; Guidorzi, C.; Morsony, B. J.; Ray, A.; Chomiuk, L.

    2014-12-20

    Deep, late-time X-ray observations of the relativistic, engine-driven, type Ic SN2012ap allow us to probe the nearby environment of the explosion and reveal the unique properties of relativistic supernova explosions (SNe). We find that on a local scale of ?0.01 pc the environment was shaped directly by the evolution of the progenitor star with a pre-explosion mass-loss rate of M-dot <510{sup ?6} M{sub ?} yr{sup ?1}, in line with gamma-ray bursts (GRBs) and the other relativistic SN 2009bb. Like sub-energetic GRBs, SN2012ap is characterized by a bright radio emission and evidence for mildly relativistic ejecta. However, its late-time (?t ? 20 days) X-ray emission is ?100 times fainter than the faintest sub-energetic GRB at the same epoch, with no evidence for late-time central engine activity. These results support theoretical proposals that link relativistic SNe like 2009bb and 2012ap with the weakest observed engine-driven explosions, where the jet barely fails to break out. Furthermore, our observations demonstrate that the difference between relativistic SNe and sub-energetic GRBs is intrinsic and not due to line-of-sight effects. This phenomenology can either be due to an intrinsically shorter-lived engine or to a more extended progenitor in relativistic SNe.

  16. Centralized Analysis of Local Data, With Dollars and Lives on the Line: Lessons From The Home Radon Experience

    SciTech Connect (OSTI)

    Price, PhillipN.; Gelman, Andrew

    2014-11-24

    In this chapter we elucidate four main themes. The first is that modern data analyses, including "Big Data" analyses, often rely on data from different sources, which can present challenges in constructing statistical models that can make effective use of all of the data. The second theme is that although data analysis is usually centralized, frequently the final outcome is to provide information or allow decision-making for individuals. Third, data analyses often have multiple uses by design: the outcomes of the analysis are intended to be used by more than one person or group, for more than one purpose. Finally, issues of privacy and confidentiality can cause problems in more subtle ways than are usually considered; we will illustrate this point by discussing a case in which there is substantial and effective political opposition to simply acknowledging the geographic distribution of a health hazard. A researcher analyzes some data and learns something important. What happens next? What does it take for the results to make a difference in people's lives? In this chapter we tell a story - a true story - about a statistical analysis that should have changed government policy, but didn't. The project was a research success that did not make its way into policy, and we think it provides some useful insights into the interplay between locally-collected data, statistical analysis, and individual decision making.

  17. Explorer-II: Wireless Self-Powered Visual and NDE Robotic Inspection System for Live Gas Distribution Mains

    SciTech Connect (OSTI)

    Carnegie Mellon University

    2008-09-30

    Carnegie Mellon University (CMU) under contract from Department of Energy/National Energy Technology Laboratory (DoE/NETL) and co-funding from the Northeast Gas Association (NGA), has completed the overall system design, field-trial and Magnetic Flux Leakage (MFL) sensor evaluation program for the next-generation Explorer-II (X-II) live gas main Non-destructive Evaluation (NDE) and visual inspection robot platform. The design is based on the Explorer-I prototype which was built and field-tested under a prior (also DoE- and NGA co-funded) program, and served as the validation that self-powered robots under wireless control could access and navigate live natural gas distribution mains. The X-II system design ({approx}8 ft. and 66 lbs.) was heavily based on the X-I design, yet was substantially expanded to allow the addition of NDE sensor systems (while retaining its visual inspection capability), making it a modular system, and expanding its ability to operate at pressures up to 750 psig (high-pressure and unpiggable steel-pipe distribution mains). A new electronics architecture and on-board software kernel were added to again improve system performance. A locating sonde system was integrated to allow for absolute position-referencing during inspection (coupled with external differential GPS) and emergency-locating. The power system was upgraded to utilize lithium-based battery-cells for an increase in mission-time. The resulting robot-train system with CAD renderings of the individual modules. The system architecture now relies on a dual set of end camera-modules to house the 32-bit processors (Single-Board Computer or SBC) as well as the imaging and wireless (off-board) and CAN-based (on-board) communication hardware and software systems (as well as the sonde-coil and -electronics). The drive-module (2 ea.) are still responsible for bracing (and centering) to drive in push/pull fashion the robot train into and through the pipes and obstacles. The steering modules and their arrangement, still allow the robot to configure itself to perform any-angle (up to 90 deg) turns in any orientation (incl. vertical), and enable the live launching and recovery of the system using custom fittings and a (to be developed) launch-chamber/-tube. The battery modules are used to power the system, by providing power to the robot's bus. The support modules perform the functions of centration for the rest of the train as well as odometry pickups using incremental encoding schemes. The electronics architecture is based on a distributed (8-bit) microprocessor architecture (at least 1 in ea. module) communicating to a (one of two) 32-bit SBC, which manages all video-processing, posture and motion control as well as CAN and wireless communications. The operator controls the entire system from an off-board (laptop) controller, which is in constant wireless communication with the robot train in the pipe. The sensor modules collect data and forward it to the robot operator computer (via the CAN-wireless communications chain), who then transfers it to a dedicated NDE data-storage and post-processing computer for further (real-time or off-line) analysis. The prototype robot system was built and tested indoors and outdoors, outfitted with a Remote-Field Eddy Current (RFEC) sensor integrated as its main NDE sensor modality. An angled launcher, allowing for live launching and retrieval, was also built to suit custom angled launch-fittings from TDW. The prototype vehicle and launcher systems are shown. The complete system, including the in-pipe robot train, launcher, integrated NDE-sensor and real-time video and control console and NDE-data collection and -processing and real-time display, were demonstrated to all sponsors prior to proceeding into final field-trials--the individual components and setting for said acceptance demonstration are shown. The launcher-tube was also used to verify that the vehicle system is capable of operating in high-pressure environments, and is safely deployable using proper evacuating/purging techniques for operation in the po

  18. The Potential Role of the Thorium Fuel Cycle in Reducing the Radiotoxicity of Long-Lived Waste - 13477

    SciTech Connect (OSTI)

    Hesketh, Kevin; Thomas, Mike

    2013-07-01

    The thorium (or more accurately the Th-232/U-233) fuel cycle is attracting growing interest world wide and one reason for this is the reduced radiotoxicity of long-lived waste, with the Th- 232/U-233 fuel cycle often being justified partly on the grounds of low radiotoxicity for long cooling times. This paper considers the evolution of heavy metal radiotoxicity in a Molten Salt Fast Reactor (MSFR) operating a closed Th-232/U-233 cycle during different operational phases. The paper shows that even in the MSFR core, the equilibrium radiotoxicity of the thorium fuel cycle is only reached after almost 100 years of operation. MSFR was chosen because it has many theoretical advantages that favour the Th-232/U-233 fuel cycle. Conventional solid fuel systems would be expected to behave similarly, but with even longer timescales and therefore the MSFR cycle can be used to define the limits of what is practically achievable. The results are used to argue the case that a fair approach to justifying the Th-232/U-233 breeder cycle should not quote the long term equilibrium radiotoxicity, but rather the somewhat less favourable radiotoxicity that could be achieved within the operational lifetime of the first generation of Th-232/U-233 breeder reactors. (authors)

  19. Search for Heavy, Long-Lived Neutralinos that Decay to Photons at CDF II Using Photon Timing

    SciTech Connect (OSTI)

    Aaltonen, T.; Adelman, J.; Akimoto, T.; Albrow, M.G.; Alvarez Gonzalez, B.; Amerio, S.; Amidei, D.; Anastassov, A.; Annovi, A.; Antos, J.; Aoki, M.; /Illinois U., Urbana /Fermilab

    2008-04-01

    The authors present the results of the first hadron collider search for heavy, long-lived neutralinos that decay via {tilde {chi}}{sub 1}{sup 0} {yields} {gamma}{tilde G} in gauge-mediated supersymmetry breaking models. Using an integrated luminosity of 570 {+-} 34 pb{sup -1} of p{bar p} collisions at {radical}s = 1.96 TeV, they select {gamma}+jet+missing transverse energy candidate events based on the arrival time of a high-energy photon at the electromagnetic calorimeter as measured with a timing system that was recently installed on the CDF II detector. They find 2 events, consistent with the background estimate of 1.3 {+-} 0.7 events. While the search strategy does not rely on model-specific dynamics, they set cross section limits and place the world-best 95% C.L. lower limit on the {tilde {chi}}{sub 1}{sup 0} mass of 101 GeV/c{sup 2} at {tau}{sub {tilde {chi}}{sub 1}{sup 0}} = 5 ns.

  20. Test of electron beam technology on Savannah River Laboratory low-activity aqueous waste for destruction of benzene, benzene derivatives, and bacteria

    SciTech Connect (OSTI)

    Dougal, R.A.

    1993-08-01

    High energy radiation was studied as a means for destroying hazardous organic chemical wastes. Tests were conducted at bench scale with a {sup 60}Co source, and at full scale (387 l/min) with a 1.5 MV electron beam source. Bench scale tests for both benzene and phenol included 32 permutations of water quality factors. For some water qualities, as much as 99.99% of benzene or 90% of phenol were removed by 775 krads of {sup 60}Co irradiation. Full scale testing for destruction of benzene in a simulated waste-water mix showed loss of 97% of benzene following an 800 krad dose and 88% following a 500 krad dose. At these loss rates, approximately 5 Mrad of electron beam irradiation is required to reduce concentrations from 100 g/l to drinking water quality (5 {mu}g/l). Since many waste streams are also inhabited by bacterial populations which may affect filtering operations, the effect of irradiation on those populations was also studied. {sup 60}Co and electron beam irradiation were both lethal to the bacteria studied at irradiation levels far lower than were necessary to remove organic contaminants.

  1. Phages of lactic acid bacteria: The role of genetics in understanding phage-host interactions and their co-evolutionary processes

    SciTech Connect (OSTI)

    Mahony, Jennifer; Ainsworth, Stuart; Stockdale, Stephen; Sinderen, Douwe van

    2012-12-20

    Dairy fermentations are among the oldest food processing applications, aimed at preservation and shelf-life extension through the use of lactic acid bacteria (LAB) starter cultures, in particular strains of Lactococcus lactis, Streptococcus thermophilus, Lactobacillus spp. and Leuconostoc spp. Traditionally this was performed by continuous passaging of undefined cultures from a finished fermentation to initiate the next fermentation. More recently, consumer demands on consistent and desired flavours and textures of dairy products have led to a more defined approach to such processes. Dairy (starter) companies have responded to the need to define the nature and complexity of the starter culture mixes, and dairy fermentations are now frequently based on defined starter cultures of low complexity, where each starter component imparts specific technological properties that are desirable to the product. Both mixed and defined starter culture approaches create the perfect environment for the proliferation of (bacterio)phages capable of infecting these LAB. The repeated use of the same starter cultures in a single plant, coupled to the drive towards higher and consistent production levels, increases the risk and negative impact of phage infection. In this review we will discuss recent advances in tracking the adaptation of phages to the dairy industry, the advances in understanding LAB phage-host interactions, including evolutionary and genomic aspects.

  2. MicroChip Imager Module for Recognition of Microorganisms

    Energy Science and Technology Software Center (OSTI)

    2001-01-01

    The MicroChip Reader for Cereus Group takes the table of intensities of hybridization signals produced by the MicroChip Imager software and evokes a series of steps designed to recognize the pattern of intensities specific to a particular Cereus subgroup. Seven subgroups of the Cereus group can be identified by particular features of their RNA sequence. The Reader also provides statistics documenting how well its conclusion is confirmed by the hybridization signals. At the user’s request,more »the Reader can list every recognition step utilized so that the user can verify the recognition process manually if desired.« less

  3. Closer look at microorganism provides insight on carbon cycling...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Brian Grabowski at media@anl.gov or (630) 252-1232. Connect Find an Argonne expert by subject. Follow Argonne on Twitter, Facebook, Google+ and LinkedIn. For inquiries on...

  4. Recent activities for ?-decay half-lives and ?-delayed neutron emission of very neutron-rich isotopes

    SciTech Connect (OSTI)

    Dillmann, Iris; Abriola, Daniel; Singh, Balraj

    2014-05-02

    Beta-delayed neutron (?n) emitters play an important, two-fold role in the stellar nucleosynthesis of heavy elements in the 'rapid neutron-capture process' (r process). On one hand they lead to a detour of the material ?-decaying back to stability. On the other hand, the released neutrons increase the neutron-to-seed ratio, and are re-captured during the freeze-out phase and thus influence the final solar r-abundance curve. A large fraction of the isotopes inside the r-process reaction path are not yet experimentally accessible and are located in the (experimental) 'Terra Incognita'. With the next generation of fragmentation and ISOL facilities presently being built or already in operation, one of the main motivation of all projects is the investigation of these very neutron-rich isotopes. A short overview of one of the planned programs to measure ?n-emitters at the limits of the presently know isotopes, the BRIKEN campaign (Beta delayed neutron emission measurements at RIKEN) will be given. Presently, about 600 ?-delayed one-neutron emitters are accessible, but only for a third of them experimental data are available. Reaching more neutron-rich isotopes means also that multiple neutron-emission becomes the dominant decay mechanism. About 460 ?-delayed two-, three-or four-neutron emitters are identified up to now but for only 30 of them experimental data about the neutron branching ratios are available, most of them in the light mass region below A=30. The International Atomic and Energy Agency (IAEA) has identified the urgency and picked up this topic recently in a 'Coordinated Research Project' on a 'Reference Database for Beta-Delayed Neutron Emission Data'. This project will review, compile, and evaluate the existing data for neutron-branching ratios and half-lives of ?-delayed neutron emitters and help to ensure a reliable database for the future discoveries of new isotopes and help to constrain astrophysical and theoretical models.

  5. Fluorescent CdSe/ZnS nanocrystal-peptide conjugates for long-term, nontoxic imaging and nuclear targeting in living cells

    SciTech Connect (OSTI)

    Chen, Fanqing; Gerion, Daniele

    2004-06-14

    One of the biggest challenges in cell biology is the imaging of living cells. For this purpose, the most commonly used visualization tool is fluorescent markers. However, conventional labels, such as organic fluorescent dyes or green fluorescent proteins (GFP), lack the photostability to allow the tracking of cellular events that happen over minutes to days. In addition, they are either toxic to cells (dyes), or difficult to construct and manipulate (GFP). We report here the use of a new class of fluorescent labels, silanized CdSe/ZnS nanocrystal-peptide conjugates, for imaging the nuclei of living cells. CdSe/ZnS nanocrystals, or so called quantum dots (qdots), are extremely photostable, and have been used extensively in cellular imaging of fixed cells. However, most of the studies about living cells so far have been concerned only with particle entry into the cytoplasm or the localization of receptors on the cell membrane. Specific targeting of qdots to the nucleus of living cells ha s not been reported in previous studies, due to the lack of a targeting mechanism and proper particle size. Here we demonstrate for the first time the construction of a CdSe/ZnS nanocrystal-peptide conjugate that carries the SV40 large T antigen nuclear localization signal (NLS), and the transfection of the complex into living cells. By a novel adaptation of commonly used cell transfection techniques for qdots, we were able to introduce and retain the NLS-qdots conjugate in living cells for up to a week without detectable negative cellular effects. Moreover, we can visualize the movement of the CdSe/ZnS nanocrystal-peptide conjugates from cytoplasm to the nucleus, and the accumulation of the complex in the cell nucleus, over a long observation time period. This report opens the door for using qdots to visualize long-term biological events that happen in the cell nucleus, and provides a new nontoxic, long-term imaging platform for cell nuclear processes.

  6. Metabolic engineering in methanotrophic bacteria

    SciTech Connect (OSTI)

    Kalyuzhnaya, MG; Puri, AW; Lidstrom, ME

    2015-05-01

    Methane, as natural gas or biogas, is the least expensive source of carbon for (bio)chemical synthesis. Scalable biological upgrading of this simple alkane to chemicals and fuels can bring new sustainable solutions to a number of industries with large environmental footprints, such as natural gas/petroleum production, landfills, wastewater treatment, and livestock. Microbial biocatalysis with methane as a feedstock has been pursued off and on for almost a half century, with little enduring success. Today, biological engineering and systems biology provide new opportunities for metabolic system modulation and give new optimism to the concept of a methane-based bio-industry. Here we present an overview of the most recent advances pertaining to metabolic engineering of microbial methane utilization. Some ideas concerning metabolic improvements for production of acetyl-CoA and pyruvate, two main precursors for bioconversion, are presented. We also discuss main gaps in the current knowledge of aerobic methane utilization, which must be solved in order to release the full potential of methane-based biosystems. (C) 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

  7. Search for heavy long-lived multi-charged particles in pp collisions at ?s = 8 TeV using the ATLAS detector

    SciTech Connect (OSTI)

    2015-08-08

    A search for heavy long-lived multi-charged particles is performed using the ATLAS detector at the LHC. Data collected in 2012 at ?s = 8 TeV from pp collisions corresponding to an integrated luminosity of 20.3 fb-1 are examined. Particles producing anomalously high ionisation, consistent with long-lived massive particles with electric charges from |q| = 2e to |q| = 6e are searched for. No signal candidate events are observed, and 95 % confidence level cross-section upper limits are interpreted as lower mass limits for a DrellYan production model. The mass limits range between 660 and 785 GeV.

  8. Real-time Molecular Study of Bystander Effects of Low dose Low LET radiation Using Living Cell Imaging and Nanoparticale Optics

    SciTech Connect (OSTI)

    Natarajan, Mohan; Xu, Nancy R; Mohan, Sumathy

    2013-06-03

    In this study two novel approaches are proposed to investigate precisely the low dose low LET radiation damage and its effect on bystander cells in real time. First, a flow shear model system, which would provide us a near in vivo situation where endothelial cells in the presence of extra cellular matrix experiencing continuous flow shear stress, will be used. Endothelial cells on matri-gel (simulated extra cellular matrix) will be subjected to physiological flow shear (that occurs in normal blood vessels). Second, a unique tool (Single nano particle/single live cell/single molecule microscopy and spectroscopy; Figure A) will be used to track the molecular trafficking by single live cell imaging. Single molecule chemical microscopy allows one to single out and study rare events that otherwise might be lost in assembled average measurement, and monitor many target single molecules simultaneously in real-time. Multi color single novel metal nanoparticle probes allow one to prepare multicolor probes (Figure B) to monitor many single components (events) simultaneously and perform multi-complex analysis in real-time. These nano-particles resist to photo bleaching and hence serve as probes for unlimited timeframe of analysis. Single live cell microscopy allows one to image many single cells simultaneously in real-time. With the combination of these unique tools, we will be able to study under near-physiological conditions the cellular and sub-cellular responses (even subtle changes at one molecule level) to low and very low doses of low LET radiation in real time (milli-second or nano-second) at sub-10 nanometer spatial resolution. This would allow us to precisely identify, at least in part, the molecular mediators that are responsible of radiation damage in the irradiated cells and the mediators that are responsible for initiating the signaling in the neighboring cells. Endothelial cells subjected to flow shear (2 dynes/cm2 or 16 dynes/cm2) and exposed to 0.1, 1 and 10 cGy on coverslips will be examined for (a) low LET radiation-induced alterations of cellular function and its physiological relevance in real time; and (b) radiation damage triggered bystander effect on the neighboring unirradiated cells. First, to determine the low LET radiation induced alteration of cellular function we will examine: (i) the real time transformation of single membrane transporters in single living cells; (ii) the pump efficiency of membrane efflux pump of live cells in real time at the molecular level; (iii) the kinetics of single-ligand receptor interaction on single live cell surface (Figure C); and (iv) alteration in chromosome replication in living cell. Second, to study the radiation triggered bystander responses, we will examine one of the key signaling pathway i.e. TNF- alpha/NF-kappa B mediated signaling. TNF-alpha specific nano particle sensors (green) will be developed to detect the releasing dynamics, transport mechanisms and ligand-receptor binding on live cell surface in real time. A second sensor (blue) will be developed to simultaneously monitor the track of NF-kB inside the cell. The proposed nano-particle optics approach would complement our DOE funded study on biochemical mechanisms of TNF-alpha- NF-kappa B-mediated bystander effect.

  9. The secret lives of Cepheids: evolutionary changes and pulsation-induced shock heating in the prototype classical Cepheid ? Cep

    SciTech Connect (OSTI)

    Engle, Scott G.; Guinan, Edward F. [Department of Astrophysics and Planetary Science, Villanova University, Villanova, PA 19085 (United States); Harper, Graham M. [School of Physics, Trinity College Dublin, College Green, Dublin 2 (Ireland); Neilson, Hilding R. [Department of Physics and Astronomy, East Tennessee State University, Box 70652, Johnson City, TN 37614 (United States); Evans, Nancy Remage, E-mail: scott.engle@villanova.edu [Smithsonian Astrophysical Observatory, MS 4, 60 Garden Street, Cambridge, MA 02138 (United States)

    2014-10-10

    Over the past decade, the Secret Lives of Cepheids (SLiC) program has been carried out at Villanova University to study aspects and behaviors of classical Cepheids that are still not well understood. In this, the first of several planned papers on program Cepheids, we report the current results for ? Cep, the Cepheid prototype. Ongoing photometry has been obtained to search for changes in the pulsation period, light-curve morphology, and amplitude. Combining our photometry with the times of maximum light compilation by Berdnikov et al. returns a small period change of dP/dt ?0.1006 0.0002 s yr{sup -1}. There is also evidence for a gradual light amplitude increase of ?0.011 mag (V band) and ?0.012 mag (B band) per decade over the last ?50 years. In addition, Hubble Space Telescope (HST) Cosmic Origins Spectrograph (COS) UV spectrophotometry and XMM-Newton X-ray data were carried out to investigate the high-temperature plasmas present above the Cepheid photospheres. In total, from the five visits (eight exposures) with XMM-Newton, ? Cep is found to be a soft X-ray source (L {sub X} (0.3-2 keV) ?4.5-13 10{sup 28} erg s{sup -1}) with peak flux at kT = 0.6-0.9 keV. The X-ray activity is found to vary, possibly in phase with the stellar pulsations. From 2010-2013, nine observations of ? Cep were carried out with HST-COS. The UV emissions are also variable and well phased with the stellar pulsations. Maximum UV line emissions occur near, or slightly before, maximum optical light, varying by as much as 20 times. This variability shows that pulsation-induced shock heating plays a significant role in Cepheid atmospheres, possibly in addition to a quiescent, magnetic heating. The results of this study show Cepheid atmospheres to be rather complex and dynamic.

  10. Search for long-lived particles that decay into final states containing two electrons or two muons in proton-proton collisions at s=8TeV

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Khachatryan, V.; Sirunyan, A. M.; Tumasyan, A.; Adam, W.; Bergauer, T.; Dragicevic, M.; Erö, J.; Friedl, M.; Frühwirth, R.; Ghete, V. M.; et al

    2015-03-18

    A search is performed for long-lived particles that decay into final states that include a pair of electrons or a pair of muons. The experimental signature is a distinctive topology consisting of a pair of charged leptons originating from a displaced secondary vertex. Events corresponding to an integrated luminosity of 19.6 (20.5)  fb⁻¹ in the electron (muon) channel were collected with the CMS detector at the CERN LHC in proton-proton collisions at √s = 8  TeV. No significant excess is observed above standard model expectations. Upper limits on the product of the cross section and branching fraction of such a signal are presentedmore » as a function of the long-lived particle’s mean proper decay length. The limits are presented in an approximately model-independent way, allowing them to be applied to a wide class of models yielding the above topology. Over much of the investigated parameter space, the limits obtained are the most stringent to date. In the specific case of a model in which a Higgs boson in the mass range 125–1000  GeV/c² decays into a pair of long-lived neutral bosons in the mass range 20–350  GeV/c², each of which can then decay to dileptons, the upper limits obtained are typically in the range 0.2–10 fb for mean proper decay lengths of the long-lived particles in the range 0.01–100 cm. In the case of the lowest Higgs mass considered (125  GeV/c²), the limits are in the range 2–50 fb. These limits are sensitive to Higgs boson branching fractions as low as 10⁻⁴.« less

  11. Searches for heavy long-lived charged particles with the ATLAS detector in proton-proton collisions at √s = 8 TeV

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Aad, G.

    2015-01-14

    Searches for heavy long-lived charged particles are performed using a data sample of 19.1 fb-1 from proton-proton collisions at a centre-of-mass energy of \\( \\sqrt{s}=8 \\) TeV collected by the ATLAS detector at the Large Hadron Collider. No excess is observed above the estimated background and limits are placed on the mass of long-lived particles in various supersymmetric models. Long-lived tau sleptons in models with gauge-mediated symmetry breaking are excluded up to masses between 440 and 385 GeV for tan β between 10 and 50, with a 290 GeV limit in the case where only direct tau slepton production ismore » considered. In the context of simplified LeptoSUSY models, where sleptons are stable and have a mass of 300 GeV, squark and gluino masses are excluded up to a mass of 1500 and 1360 GeV, respectively. Directly produced charginos, in simplified models where they are nearly degenerate to the lightest neutralino, are excluded up to a mass of 620 GeV. As a result, R-hadrons, composites containing a gluino, bottom squark or top squark, are excluded up to a mass of 1270, 845 and 900 GeV, respectively, using the full detector; and up to a mass of 1260, 835 and 870 GeV using an approach disregarding information from the muon spectrometer.« less

  12. Searches for heavy long-lived charged particles with the ATLAS detector in proton-proton collisions at ?s = 8 TeV

    SciTech Connect (OSTI)

    Aad, G.

    2015-01-14

    Searches for heavy long-lived charged particles are performed using a data sample of 19.1 fb-1 from proton-proton collisions at a centre-of-mass energy of \\( \\sqrt{s}=8 \\) TeV collected by the ATLAS detector at the Large Hadron Collider. No excess is observed above the estimated background and limits are placed on the mass of long-lived particles in various supersymmetric models. Long-lived tau sleptons in models with gauge-mediated symmetry breaking are excluded up to masses between 440 and 385 GeV for tan ? between 10 and 50, with a 290 GeV limit in the case where only direct tau slepton production is considered. In the context of simplified LeptoSUSY models, where sleptons are stable and have a mass of 300 GeV, squark and gluino masses are excluded up to a mass of 1500 and 1360 GeV, respectively. Directly produced charginos, in simplified models where they are nearly degenerate to the lightest neutralino, are excluded up to a mass of 620 GeV. As a result, R-hadrons, composites containing a gluino, bottom squark or top squark, are excluded up to a mass of 1270, 845 and 900 GeV, respectively, using the full detector; and up to a mass of 1260, 835 and 870 GeV using an approach disregarding information from the muon spectrometer.

  13. Bioremediation of nanomaterials

    DOE Patents [OSTI]

    Chen, Frank Fanqing; Keasling, Jay D; Tang, Yinjie J

    2013-05-14

    The present invention provides a method comprising the use of microorganisms for nanotoxicity study and bioremediation. In some embodiment, the microorganisms are bacterial organisms such as Gram negative bacteria, which are used as model organisms to study the nanotoxicity of the fullerene compounds: E. coli W3110, a human related enterobacterium and Shewanella oneidensis MR-1, an environmentally important bacterium with versatile metabolism.

  14. Long-lived nanosecond spin relaxation and spin coherence of electrons in monolayer MoS2 and WS2

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Yang, Luyi; Sinitsyn, Nikolai A.; Chen, Weibing; Yuan, Jiangtan; Zhang, Jing; Lou, Jun; Crooker, Scott  A.

    2015-08-03

    The recently discovered monolayer transition metal dichalcogenides (TMDCs) provide a fertile playground to explore new coupled spin–valley physics. Although robust spin and valley degrees of freedom are inferred from polarized photoluminescence (PL) experiments PL timescales are necessarily constrained by short-lived (3–100 ps) electron–hole recombination9, 10. Direct probes of spin/valley polarization dynamics of resident carriers in electron (or hole)-doped TMDCs, which may persist long after recombination ceases, are at an early stage. Here we directly measure the coupled spin–valley dynamics in electron-doped MoS2 and WS2 monolayers using optical Kerr spectroscopy, and reveal very long electron spin lifetimes, exceeding 3 ns atmore » 5 K (2-3 orders of magnitude longer than typical exciton recombination times). In contrast with conventional III–V or II–VI semiconductors, spin relaxation accelerates rapidly in small transverse magnetic fields. Supported by a model of coupled spin–valley dynamics, these results indicate a novel mechanism of itinerant electron spin dephasing in the rapidly fluctuating internal spin–orbit field in TMDCs, driven by fast inter-valley scattering. Additionally, a long-lived spin coherence is observed at lower energies, commensurate with localized states. These studies provide insight into the physics underpinning spin and valley dynamics of resident electrons in atomically thin TMDCs.« less

  15. Live Cells as Dynamic Laboratories: Time Lapse Raman Spectral Microscopy of Nanoparticles with Both IgE Targeting and pH-Sensing Functions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Nowak-Lovato, Kristy L.; Rector, Kirk D.

    2012-01-01

    Tmore » his review captures the use of live cells as dynamic microlaboratories through implementation of labeled nanoparticles (nanosensors) that have both sensing and targeting functions. The addition of 2,4-ε-dinitrophenol-L-lysine (DNP) as a FcεRI targeting ligand and 4-mercaptopyridine (4-MPy) as a pH-sensing ligand enables spatial and temporal monitoring of FcεRI receptors and their pH environment within the endocytic pathway. To ensure reliability, the sensor is calibrated in vivo using the ionophore nigericin and standard buffer solutions to equilibrate the external [ H + ] concentration with that of the cell compartments. This review highlights the nanosensors, ability to traffic and respond to pH of receptor-bound nanosensors (1) at physiological temperature ( 37 ° C ) versus room temperature ( 25 ° C ) , (2) after pharmacological treatment with bafilomycin, an H + ATPase pump inhibitor, or amiloride, an inhibitor of Na + / H + exchange, and (3) in response to both temperature and pharmacological treatment. Whole-cell, time lapse images are demonstrated to show the ability to transform live cells into dynamic laboratories to monitor temporal and spatial endosomal pH. The versatility of these probes shows promise for future applications relevant to intracellular trafficking and intelligent drug design.« less

  16. Triggering collapse of the presolar dense cloud core and injecting short-lived radioisotopes with a shock wave. III. Rotating three-dimensional cloud cores

    SciTech Connect (OSTI)

    Boss, Alan P.; Keiser, Sandra A.

    2014-06-10

    A key test of the supernova triggering and injection hypothesis for the origin of the solar system's short-lived radioisotopes is to reproduce the inferred initial abundances of these isotopes. We present here the most detailed models to date of the shock wave triggering and injection process, where shock waves with varied properties strike fully three-dimensional, rotating, dense cloud cores. The models are calculated with the FLASH adaptive mesh hydrodynamics code. Three different outcomes can result: triggered collapse leading to fragmentation into a multiple protostar system; triggered collapse leading to a single protostar embedded in a protostellar disk; or failure to undergo dynamic collapse. Shock wave material is injected into the collapsing clouds through Rayleigh-Taylor fingers, resulting in initially inhomogeneous distributions in the protostars and protostellar disks. Cloud rotation about an axis aligned with the shock propagation direction does not increase the injection efficiency appreciably, as the shock parameters were chosen to be optimal for injection even in the absence of rotation. For a shock wave from a core-collapse supernova, the dilution factors for supernova material are in the range of ?10{sup 4} to ?3 10{sup 4}, in agreement with recent laboratory estimates of the required amount of dilution for {sup 60}Fe and {sup 26}Al. We conclude that a type II supernova remains as a promising candidate for synthesizing the solar system's short-lived radioisotopes shortly before their injection into the presolar cloud core by the supernova's remnant shock wave.

  17. Long-lived nanosecond spin relaxation and spin coherence of electrons in monolayer MoS2 and WS2

    SciTech Connect (OSTI)

    Yang, Luyi; Sinitsyn, Nikolai A.; Chen, Weibing; Yuan, Jiangtan; Zhang, Jing; Lou, Jun; Crooker, Scott  A.

    2015-08-03

    The recently discovered monolayer transition metal dichalcogenides (TMDCs) provide a fertile playground to explore new coupled spin–valley physics. Although robust spin and valley degrees of freedom are inferred from polarized photoluminescence (PL) experiments PL timescales are necessarily constrained by short-lived (3–100 ps) electron–hole recombination9, 10. Direct probes of spin/valley polarization dynamics of resident carriers in electron (or hole)-doped TMDCs, which may persist long after recombination ceases, are at an early stage. Here we directly measure the coupled spin–valley dynamics in electron-doped MoS2 and WS2 monolayers using optical Kerr spectroscopy, and reveal very long electron spin lifetimes, exceeding 3 ns at 5 K (2-3 orders of magnitude longer than typical exciton recombination times). In contrast with conventional III–V or II–VI semiconductors, spin relaxation accelerates rapidly in small transverse magnetic fields. Supported by a model of coupled spin–valley dynamics, these results indicate a novel mechanism of itinerant electron spin dephasing in the rapidly fluctuating internal spin–orbit field in TMDCs, driven by fast inter-valley scattering. Additionally, a long-lived spin coherence is observed at lower energies, commensurate with localized states. These studies provide insight into the physics underpinning spin and valley dynamics of resident electrons in atomically thin TMDCs.

  18. Ultrasensitive search for long-lived superheavy nuclides in the mass range A=288 to A=300 in natural Pt, Pb, and Bi

    SciTech Connect (OSTI)

    Dellinger, F.; Forstner, O.; Golser, R.; Priller, A.; Steier, P.; Wallner, A.; Winkler, G.; Kutschera, W.

    2011-06-15

    Theoretical models of superheavy elements (SHEs) predict a region of increased stability around the proton and neutron shell closures of Z = 114 and N = 184. Therefore a sensitive search for nuclides in the mass range from A = 288 to A = 300 was performed in natural platinum, lead, and bismuth, covering long-lived isotopes of Eka-Pt (Ds, Z = 110), Eka-Pb (Z = 114), and Eka-Bi (Z = 115). Measurements with accelerator mass spectrometry (AMS) at the Vienna Environmental Research Accelerator (VERA) established upper limits for these SHE isotopes in Pt, Pb, and Bi with abundances of <2x10{sup -15}, <5x10{sup -14}, and <5x10{sup -13}, respectively. These results complement earlier searches for SHEs with AMS at VERA in natural thorium and gold, which now amounts to a total number of 37 SHE nuclides having been explored with AMS. In none of our measurements was evidence found for the existence of SHEs in nature at the reported sensitivity level. Even though a few events were observed in the window for {sup 293}Eka-Bi, a particularly strong pileup background did not allow a definite SHE isotope identification. The present result sets limits on nuclides around the center of the island of stability, essentially ruling out the existence of SHE nuclides with half-lives longer than {approx}150 million years.

  19. Quo vadis? Microbial profiling revealed strong effects of cleanroom maintenance and routes of contamination in indoor environments

    SciTech Connect (OSTI)

    Moissl-Eichinger, Christine; Auerbach, Anna K.; Probst, Alexander J.; Mahnert, Alexander; Tom, Lauren; Piceno, Yvette; Andersen, Gary L.; Venkateswaran, Kasthuri; Rettberg, Petra; Barczyk, Simon; Pukall, Rüdiger; Berg, Gabriele

    2015-03-17

    Space agencies maintain highly controlled cleanrooms to ensure the demands of planetary protection. To study potential effects of microbiome control, we analyzed microbial communities in two particulate-controlled cleanrooms (ISO 5 and ISO 8) and two vicinal uncontrolled areas (office, changing room) by cultivation and 16S rRNA gene amplicon analysis (cloning, pyrotagsequencing, and PhyloChip G3 analysis). Maintenance procedures affected the microbiome on total abundance and microbial community structure concerning richness, diversity and relative abundance of certain taxa. Cleanroom areas were found to be mainly predominated by potentially human-associated bacteria; archaeal signatures were detected in every area. Results indicate that microorganisms were mainly spread from the changing room (68%) into the cleanrooms, potentially carried along with human activity. The numbers of colony forming units were reduced by up to ~400 fold from the uncontrolled areas towards the ISO 5 cleanroom, accompanied with a reduction of the living portion of microorganisms from 45% (changing area) to 1% of total 16S rRNA gene signatures as revealed via propidium monoazide treatment of the samples. Our results demonstrate the strong effects of cleanroom maintenance on microbial communities in indoor environments and can be used to improve the design and operation of biologically controlled cleanrooms.

  20. Quo vadis? Microbial profiling revealed strong effects of cleanroom maintenance and routes of contamination in indoor environments

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Moissl-Eichinger, Christine; Auerbach, Anna K.; Probst, Alexander J.; Mahnert, Alexander; Tom, Lauren; Piceno, Yvette; Andersen, Gary L.; Venkateswaran, Kasthuri; Rettberg, Petra; Barczyk, Simon; et al

    2015-03-17

    Space agencies maintain highly controlled cleanrooms to ensure the demands of planetary protection. To study potential effects of microbiome control, we analyzed microbial communities in two particulate-controlled cleanrooms (ISO 5 and ISO 8) and two vicinal uncontrolled areas (office, changing room) by cultivation and 16S rRNA gene amplicon analysis (cloning, pyrotagsequencing, and PhyloChip G3 analysis). Maintenance procedures affected the microbiome on total abundance and microbial community structure concerning richness, diversity and relative abundance of certain taxa. Cleanroom areas were found to be mainly predominated by potentially human-associated bacteria; archaeal signatures were detected in every area. Results indicate that microorganisms weremore » mainly spread from the changing room (68%) into the cleanrooms, potentially carried along with human activity. The numbers of colony forming units were reduced by up to ~400 fold from the uncontrolled areas towards the ISO 5 cleanroom, accompanied with a reduction of the living portion of microorganisms from 45% (changing area) to 1% of total 16S rRNA gene signatures as revealed via propidium monoazide treatment of the samples. Our results demonstrate the strong effects of cleanroom maintenance on microbial communities in indoor environments and can be used to improve the design and operation of biologically controlled cleanrooms.« less

  1. Sediment studies at Bikini Atoll part 3. Inventories of some long-lived gamma-emitting radionuclides associated with lagoon surface sediments

    SciTech Connect (OSTI)

    Noshkin, V.E.

    1997-12-01

    Surface sediment samples were collected during 1979 from 87 locations in the lagoon at Bikini Atoll. The collections were made to better define the concentrations and distribution of long-lived radionuclides associated with the bottom material and to show what modifications occurred to the composition of the surface sediment from the nuclear testing program conducted by the United States at the Atoll between 1946 and 1958. This is the last of three reports on Bikini sediment studies. In this report, we discuss the concentrations and inventories of the residual long-lived gamma-emitting radionuclides in sediments from the lagoon. The gamma-emitting radionuclides detected most frequently in sediments collected in 1979, in addition to Americium-241 ({sup 241}Am) (discussed in the second report of this series), included Cesium-137 ({sup 137}Cs), Bismuth-207 ({sup 207}Bi), Europium-155 ({sup 155}Eu), and Cobalt-60 ({sup 60}Co). Other man-made, gamma-emitting radionuclides such as Europium-152,154 ({sup 152,154}Eu), Antimony-125 ({sup 125}Sb), and Rhodium-101,102m ({sup 101,102m}Rh) were occasionally measured above detection limits in sediments near test site locations. The mean inventories for {sup 137}Cs, {sup 207}Ei, {sup 155}Eu, and {sup 60}Co in the surface 4 cm of the lagoon sediment to be 1.7, 0.56, 7.76, and 0.74 TBq, respectively. By June 1997, radioactive decay would reduce these values to 1.1, 0.38, 0.62, and 0.07 TBq, respectively. Some additional loss results from a combination of different processes that continuously mobilize and return some amount of the radionuclides to the water column. The water and dissolved constituents are removed from the lagoon through channels and exchange with the surface waters of the north equatorial Pacific Ocean. Highest levels of these radionuclides are found in surface deposits lagoonward of the Bravo Crater. Lowest concentrations and inventories are associated with sediment lagoonward of the eastern reef. The quantities in the 0-4 cm surface layer are estimated to be less than 35% of the total inventory to depth in the sediment column.

  2. Radionuclide characterization and associated dose from long-lived radionuclides in close-in fallout delivered to the marine environment at Bikini and Enewetak Atoll

    SciTech Connect (OSTI)

    Noshkin, V. E.; Robison, W. L.

    1998-09-01

    Between June 1946 and October 1958, Enewetak and Bikini Atolls were used by the United States as testing grounds for 66 nuclear devices. The combined explosive yield from these tests was 107 Mt (Mt TNT equivalents). This testing produced close-in fallout debris that was contaminated with quantities of radioactive fission and particle activated products, and unspent radioactive nuclear fuel that entered the aquatic environment of the atolls. Today, the sediments in the lagoons are reservoirs for 10's of TBq of the transuranics and some long-lived fission and activation products. The larger amounts of contamination are associated with fine and coarse sediment material adjacent to the locations of the high yield explosions. Radionuclides are also distributed vertically in the sediment column to various depths in all regions of the lagoons. Concentrations greater than fallout background levels are found in filtered water sampled over several decades from all locations and depths in the lagoons. This is a direct indication that the radionuclides are continuously mobilized to solution from the solid phases. Of particular importance is the fact that the long-lived radionuclides are accumulated to different levels by indigenous aquatic plants and organisms that are used as food by resident people. One might anticipate finding continuous high contamination levels in many of the edible marine organisms from the lagoons, since the radionuclides associated with the sediments are not contained and are available to the different organisms in a relatively shallow water environment. This is not the case. We estimate that the radiological dose from consumption of the edible parts of marine foods at Enewetak and Bikini is presently about 0.05% of the total 50-year integral effective dose from all other exposure pathways that include ingestion of terrestrial foods and drinking water, external exposure and inhalation. The total radiological dose from the marine pathway is dominated by the natural radionuclides, {sup 210}Po and {sup 210}Pb. Man-made radionuclides presently contribute less than 0.3% of the dose from these natural radionuclides in the marine food chain.

  3. Secret Lives of Catalysts Revealed

    ScienceCinema (OSTI)

    Miquel Salmeron and Gabor Somorjai

    2010-01-08

    Miquel Salmeron and Gabor Somorjai of Berkeley Lab's Materials Sciences Division discuss the first-ever glimpse of nanoscale catalysts in action. More information: http://newscenter.lbl.gov/press-relea...

  4. LIVE_NSB_final.wmv

    ScienceCinema (OSTI)

    None

    2010-09-01

    National Science Bowl finals and awards at the National Building Museum in Washington D.C. Monday 5/3/2010

  5. Search for decays of stopped long-lived particles produced in protonproton collisions at ?s = 8 TeV

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Khachatryan, V.

    2015-04-11

    A search has been performed for long-lived particles that could have come to rest within the CMS detector, using the time intervals between LHC beam crossings. The existence of such particles could be deduced from observation of their decays via energy deposits in the CMS calorimeter appearing at times that are well separated from any protonproton collisions. Using a data set corresponding to an integrated luminosity of 18.6fb? of 8TeV protonproton collisions, and a search interval corresponding to 281 h of trigger livetime, 10 events are observed, with a background prediction of 13.2+3.62.5 events. Limits are presented at 95 %moreconfidence level on gluino and top squark production, for over 13 orders of magnitude in the mean proper lifetime of the stopped particle. Assuming a cloud model of R-hadron interactions, a gluino with mass ?1000GeV and a top squark with mass ?525GeV are excluded, for lifetimes between 1 ?s and 1000s. These results are the most stringent constraints on stopped particles to date.less

  6. Search for decays of stopped long-lived particles produced in protonproton collisions at ?s = 8 TeV

    SciTech Connect (OSTI)

    Khachatryan, V.

    2015-04-11

    A search has been performed for long-lived particles that could have come to rest within the CMS detector, using the time intervals between LHC beam crossings. The existence of such particles could be deduced from observation of their decays via energy deposits in the CMS calorimeter appearing at times that are well separated from any protonproton collisions. Using a data set corresponding to an integrated luminosity of 18.6fb? of 8TeV protonproton collisions, and a search interval corresponding to 281 h of trigger livetime, 10 events are observed, with a background prediction of 13.2+3.62.5 events. Limits are presented at 95 % confidence level on gluino and top squark production, for over 13 orders of magnitude in the mean proper lifetime of the stopped particle. Assuming a cloud model of R-hadron interactions, a gluino with mass ?1000GeV and a top squark with mass ?525GeV are excluded, for lifetimes between 1 ?s and 1000s. These results are the most stringent constraints on stopped particles to date.

  7. Search for decays of stopped long-lived particles produced in proton–proton collisions at $$\\sqrt{s}= 8\\,\\text {TeV} $$

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Khachatryan, Vardan

    2015-04-11

    A search has been performed for long-lived particles that could have come to rest within the CMS detector, using the time intervals between LHC beam crossings. The existence of such particles could be deduced from observation of their decays via energy deposits in the CMS calorimeter appearing at times that are well separated from any proton–proton collisions. Using a data set corresponding to an integrated luminosity of 18.6 fb-1 of 8 TeV proton–proton collisions, and a search interval corresponding to 281 h of trigger livetime, 10 events are observed, with a background prediction of 13.2+3.6 -2.5 events. Limits are presentedmore » at 95 % confidence level on gluino and top squark production, for over 13 orders of magnitude in the mean proper lifetime of the stopped particle. Assuming a cloud model of R-hadron interactions, a gluino with mass ≤1000 GeV and a top squark with mass ≤525 GeV are excluded, for lifetimes between 1 µs and 1000 s. Finally, these results are the most stringent constraints on stopped particles to date.« less

  8. A novel facility for 3D micro-irradiation of living cells in a controlled environment by MeV ions

    SciTech Connect (OSTI)

    Mckel, V. Meissl, W.; Ikeda, T.; Meissl, E.; Kobayashi, T.; Kojima, T. M.; Ogiwara, K.; Yamazaki, Y.; Clever, M.; Imamoto, N.

    2014-01-15

    We present a novel facility for micro-irradiation of living targets with ions from a 1.7 MV tandem accelerator. We show results using 1 MeV protons and 2 MeV He{sup 2+}. In contrast to common micro-irradiation facilities, which use electromagnetic or electrostatic focusing and specially designed vacuum windows, we employ a tapered glass capillary with a thin end window, made from polystyrene with a thickness of 12 ?m, for ion focusing and extraction. The capillary is connected to a beamline tilted vertically by 45, which allows for easy immersion of the extracted ions into liquid environment within a standard cell culture dish. An inverted microscope is used for simultaneously observing the samples as well as the capillary tip, while a stage-top incubator provides an appropriate environment for the samples. Furthermore, our setup allows to target volumes in cells within a ?m{sup 3} resolution, while monitoring the target in real time during and after irradiation.

  9. TRIGGERING COLLAPSE OF THE PRESOLAR DENSE CLOUD CORE AND INJECTING SHORT-LIVED RADIOISOTOPES WITH A SHOCK WAVE. II. VARIED SHOCK WAVE AND CLOUD CORE PARAMETERS

    SciTech Connect (OSTI)

    Boss, Alan P.; Keiser, Sandra A. E-mail: keiser@dtm.ciw.edu

    2013-06-10

    A variety of stellar sources have been proposed for the origin of the short-lived radioisotopes that existed at the time of the formation of the earliest solar system solids, including Type II supernovae (SNe), asymptotic giant branch (AGB) and super-AGB stars, and Wolf-Rayet star winds. Our previous adaptive mesh hydrodynamics models with the FLASH2.5 code have shown which combinations of shock wave parameters are able to simultaneously trigger the gravitational collapse of a target dense cloud core and inject significant amounts of shock wave gas and dust, showing that thin SN shocks may be uniquely suited for the task. However, recent meteoritical studies have weakened the case for a direct SN injection to the presolar cloud, motivating us to re-examine a wider range of shock wave and cloud core parameters, including rotation, in order to better estimate the injection efficiencies for a variety of stellar sources. We find that SN shocks remain as the most promising stellar source, though planetary nebulae resulting from AGB star evolution cannot be conclusively ruled out. Wolf-Rayet (WR) star winds, however, are likely to lead to cloud core shredding, rather than to collapse. Injection efficiencies can be increased when the cloud is rotating about an axis aligned with the direction of the shock wave, by as much as a factor of {approx}10. The amount of gas and dust accreted from the post-shock wind can exceed that injected from the shock wave, with implications for the isotopic abundances expected for a SN source.

  10. SSRL HEADLINES January 2009

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    SSRL: Working toward Top Off and Higher Current Workshop on Small-Angle X-ray Scattering ... Phytoplankton are microorganisms that live in the ocean surface waters and are important ...

  11. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    newly optimized version for screening protein expression in living bacteria such as E. coli, the workhorse used by many labs to express recombinant proteins. A major advantage of...

  12. Constraints on the pMSSM, AMSB model and on other models from the search for long-lived charged particles in protonproton collisions at ?s = 8 TeV

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Khachatryan, V.

    2015-07-17

    Stringent limits are set on the long-lived lepton-like sector of the phenomenological minimal supersymmetric standard model (pMSSM) and the anomaly-mediated supersymmetry breaking (AMSB) model. The limits are derived from the results presented in a recent search for long-lived charged particles in protonproton collisions, based on data collected by the CMS detector at a centre-of-mass energy of 8 TeV at the Large Hadron Collider. In the pMSSM parameter sub-space considered, 95.9 % of the points predicting charginos with a lifetime of at least 10 ns are excluded. These constraints on the pMSSM are the first obtained at the LHC. Charginos withmorea lifetime greater than 100 ns and masses up to about 800 GeV in the AMSB model are also excluded. The method described can also be used to set constraints on other models.less

  13. Constraints on the pMSSM, AMSB model and on other models from the search for long-lived charged particles in protonproton collisions at ?s = 8 TeV

    SciTech Connect (OSTI)

    Khachatryan, V.

    2015-07-17

    Stringent limits are set on the long-lived lepton-like sector of the phenomenological minimal supersymmetric standard model (pMSSM) and the anomaly-mediated supersymmetry breaking (AMSB) model. The limits are derived from the results presented in a recent search for long-lived charged particles in protonproton collisions, based on data collected by the CMS detector at a centre-of-mass energy of 8 TeV at the Large Hadron Collider. In the pMSSM parameter sub-space considered, 95.9 % of the points predicting charginos with a lifetime of at least 10 ns are excluded. These constraints on the pMSSM are the first obtained at the LHC. Charginos with a lifetime greater than 100 ns and masses up to about 800 GeV in the AMSB model are also excluded. The method described can also be used to set constraints on other models.

  14. Constraints on the pMSSM, AMSB model and on other models from the search for long-lived charged particles in proton-proton collisions at √s = 8 TeV

    SciTech Connect (OSTI)

    Khachatryan, Vardan

    2015-07-17

    Stringent limits are set on the long-lived lepton-like sector of the phenomenological minimal supersymmetric standard model (pMSSM) and the anomaly-mediated supersymmetry breaking (AMSB) model. We derived the limits from the results presented in a recent search for long-lived charged particles in proton–proton collisions, based on data collected by the CMS detector at a centre-of-mass energy of 8 TeV at the Large Hadron Collider. In the pMSSM parameter sub-space considered, 95.9 % of the points predicting charginos with a lifetime of at least 10 ns are excluded. Furthermore, these constraints on the pMSSM are the first obtained at the LHC. Charginos with a lifetime greater than 100 ns and masses up to about 800 GeV in the AMSB model are also excluded. Furthermore, the method described can also be used to set constraints on other models.

  15. Formation of short-lived radionuclides in the protoplanetary disk during late-stage irradiation of a volatile-rich reservoir

    SciTech Connect (OSTI)

    Jacobsen, B; Matzel, J; Hutcheon, I D; Krot, A N; Yin, Q -; Nagashima, K; Ramon, E; Weber, P; Ishii, H; Ciesla, F

    2010-11-30

    The origin of short-lived (t{sub 1/2} < 5 Myr) and now extinct radionuclides ({sup 10}Be, {sup 26}Al, {sup 36}Cl, {sup 41}Ca, {sup 53}Mn, {sup 60}Fe; hereafter SLRs) is fundamental to understanding the formation of the early solar system. Two distinct classes of models have been proposed to explain the origin of SLRs: (1) injection from a nearby stellar source (e.g., supernova, asymptotic giant branch star or Wolf-Rayet star) and (2) solar energetic particle irradiation of dust and gas near the proto-Sun. Recent studies have demonstrated that {sup 36}Cl was extant in the early solar system. However, its presence, initial abundance and the noticeable decoupling from {sup 26}Al raise serious questions about the origin of SLRs. Here we report {sup 36}Cl-{sup 36}S and {sup 26}Al-{sup 26}Mg systematics for wadalite and grossular, secondary minerals in a calcium-aluminum-rich inclusion (CAI) from the CV chondrite Allende that allow us to reassess the origin of SLRs. The inferred abundance of {sup 36}Cl in wadalite, corresponding to a {sup 36}Cl/{sup 35}Cl ratio of (1.81 {+-} 0.13) x 10{sup -5}, is the highest {sup 36}Cl abundance reported in any early solar system material. The high level of {sup 36}Cl in wadalite and the absence of {sup 26}Al ({sup 26}Al/{sup 27}Al {le} 3.9 x 10{sup -6}) in co-existing grossular indicates that (1) {sup 36}Cl formed by late-stage solar energetic particle irradiation and (2) the production of {sup 36}Cl, recorded by secondary minerals, is unrelated to the origin of {sup 26}Al and other SLRs ({sup 10}Be, {sup 53}Mn) recorded by primary minerals of CAIs and chondrules. We conclude that 36Cl was produced by solar energetic particle irradiation of a volatile-rich reservoir in an optically thin protoplanetary disk adjacent to the accretion region of the CV chondrite parent asteroid.

  16. Building America Case Study: Air Leakage and Air Transfer Between Garage and Living Space - Waldorf, Maryland (Fact Sheet), Technology Solutions for New and Existing Homes, Energy Efficiency & Renewable Energy (EERE)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Air Leakage and Air Transfer Between Garage and Living Space Waldorf, Maryland PROJECT INFORMATION Project Name: Ventilation Effectiveness Location: Waldorf, MD Partners: Building Science Corporation, buildingscience.com K. Hovnanian Homes, khov.com Building Component: Building and garage ventilation Application: New and retrofit; single and multifamily Year Tested: 2013 Applicable Climate Zone(s): All PERFORMANCE DATA Cost of energy efficiency measure (including labor): $0 if no garage

  17. Search for long-lived, weakly interacting particles that decay to displaced hadronic jets in proton-proton collisions at s=8  TeV with the ATLAS detector

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Aad, G.; Abbott, B.; Abdallah, J.; Abdinov, O.; Aben, R.; Abolins, M.; AbouZeid, O. S.; Abramowicz, H.; Abreu, H.; Abreu, R.; et al

    2015-07-17

    A search for the decay of neutral, weakly interacting, long-lived particles using data collected by the ATLAS detector at the LHC is presented. This analysis uses the full data set recorded in 2012: 20.3 fb-1 of proton-proton collision data at √s = 8 TeV. The search employs techniques for reconstructing decay vertices of long-lived particles decaying to jets in the inner tracking detector and muon spectrometer. Signal events require at least two reconstructed vertices. No significant excess of events over the expected background is found, and limits as a function of proper lifetime are reported for the decay of themore » Higgs boson and other scalar bosons to long-lived particles and for Hidden Valley Z' and Stealth SUSY benchmark models. The first search results for displaced decays in Z' and Stealth SUSY models are presented. The upper bounds of the excluded proper lifetimes are the most stringent to date.« less

  18. Cloning systems for Rhodococcus and related bacteria

    DOE Patents [OSTI]

    Finnerty, W.R.; Singer, M.E.

    1990-08-28

    A plasmid transformation system for Rhodococcus was developed using an Escherichia coli-Rhodococcus shuttle plasmid. Rhodococcus sp. H13-A contains three cryptic indigenous plasmids, designated pMVS100, pMVS200 and pMVS300, of 75, 19.5 and 13.4 kilobases (Kb), respectively. A 3.8 Kb restriction fragment of pMVS300 was cloned into pIJ30, a 6.3 Kb pBR322 derivative, containing the E. coli origin of replication (ori) and ampicillin resistance determinant (bla) as well as a Streptomyces gene for thiostrepton resistance, tsr. The resulting 10.1 Kb recombinant plasmid, designated pMVS301, was isolated from E. coli DH1 (pMVS301) and transformed into Rhodococcus sp. AS-50, a derivative of strain H13-A, by polyethylene glycol-assisted transformation of Rhodococcus protoplasts and selection for thiostrepton-resistant transformants. This strain was deposited with the ATCC on Feb. 1, 1988 and assigned ATCC 53719. The plasmid contains the Rhodococcus origin of replication. The plasmid and derivatives thereof can therefore be used to introduce nucleic acid sequences to and from Rhodococcus for subsequent expression and translation into protein. The isolated origin of replication can also be used in the construction of new vectors. 2 figs.

  19. Cloning systems for Rhodococcus and related bacteria

    DOE Patents [OSTI]

    Finnerty, William R.; Singer, Mary E.

    1990-01-01

    A plasmid transformation system for Rhodococcus was developed using an Escherichia coli-Rhodococcus shuttle plasmid. Rhodococcus sp. H13-A contains three cryptic indigenous plasmids, designated pMVS100, pMVS200 and pMVS300, of 75, 19.5 and 13.4 kilobases (Kb), respectively. A 3.8 Kb restriction fragment of pMVS300 was cloned into pIJ30, a 6.3 Kb pBR322 derivative, containing the E. coli origin of replication (ori) and ampicillin resistance determinant (bla) as well as a Streptomyces gene for thiostrepton resistance, tsr. The resulting 10.1 Kb recombinant plasmid, designated pMVS301, was isolated from E. coli DH1 (pMVS301) and transformed into Rhodococcus sp. AS-50, a derivative of strain H13-A, by polyethylene glycol-assisted transformation of Rhodococcus protoplasts and selection for thiostrepton-resistant transformants. This strain was deposited with the ATCC on Feb. 1, 1988 and assigned ATCC 53719. The plasmid contains the Rhodococcus origin of replication. The plasmid and derivatives thereof can therefore be used to introduce nucleic acid sequences to and from Rhodococcus for subsequent expression and translation into protein. The isolated origin of replication can also be used in the construction of new vectors.

  20. Ethanologenic bacteria with increased resistance to furfural

    DOE Patents [OSTI]

    Miller, Elliot Norman; Jarboe, Laura R.; Yomano, Lorraine P.; York, Sean W.; Shanmugam, Keelnatham; Ingram, Lonnie O'Neal

    2015-10-06

    The invention relates to bacterium that have increased resistance to furfural and methods of preparation. The invention also relates to methods of producing ethanol using the bacterium and corresponding kits.