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  1. Baby It's Cold Outside: Best Practices for Chilly Climes (101...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Baby It's Cold Outside: Best Practices for Chilly Climes (101) Baby It's Cold Outside: Best Practices for Chilly Climes (101) November 5...

  2. Baby It's Cold Outside: Best Practices for Chilly Climes (101...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Baby It's Cold Outside: Best Practices for Chilly Climes (101) Baby It's Cold Outside: Best Practices for Chilly Climes (101) Better Buildings Residential Network Peer Exchange ...

  3. Baby It's Cold Outside: Best Practices for Chilly Climes (101)

    Broader source: Energy.gov [DOE]

    Better Buildings Residential Network Peer Exchange Call Series: Baby It's Cold Outside: Best Practices for Chilly Climes (101)

  4. Science on Saturday: Taking the Universe's Baby Picture | Princeton Plasma

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Physics Lab March 12, 2016, 9:30am Science On Saturday MBG Auditorium at PPPL Science on Saturday: Taking the Universe's Baby Picture Professor David Spergel Princeton University Abstract: PDF icon D. Spergel.pdf Science_on_Saturday12Mar2016_DSpergel Contact Information Coordinator(s): Ms. Deedee Ortiz-Arias dortiz@pppl.gov Host(s): Dr. Andrew Zwicker azwicker@pppl.gov PPPL Entrance Procedures Visitor Information, Directions, Security at PPPL As a federal facility, the Princeton Plasma

  5. Do Babies Matter? The Effect of Family Formation on Men and Women in Science

    ScienceCinema (OSTI)

    Mary Ann Mason

    2010-09-01

    Mary Ann Mason, Professor of Social Welfare and Law at the University of California, Berkeley, presents "Do Babies Matter? The Effect of Family Formation on Men and Women in Science." In her talk, she discusses the difficulties of women who have a career in science or in other male-dominated professions.

  6. Do Babies Matter? The Effect of Family Formation on Men and Women in Science

    SciTech Connect (OSTI)

    Mary Ann Mason

    2007-10-30

    Mary Ann Mason, Professor of Social Welfare and Law at the University of California, Berkeley, presents "Do Babies Matter? The Effect of Family Formation on Men and Women in Science." In her talk, she discusses the difficulties of women who have a career in science or in other male-dominated professions.

  7. Fact #872: May 11, 2015 Study Finds More than 60% of Millennials and Generation Xers Use the Internet to Find a Car Dealer While Less than Half of Baby Boomers Did – Dataset

    Broader source: Energy.gov [DOE]

    Excel file and dataset for Study Finds More than 60% of Millennials and Generation Xers Use the Internet to Find a Car Dealer While Less than Half of Baby Boomers Did

  8. NNSA lab wins DOE achievement award for Beanie Baby-box inspired...

    National Nuclear Security Administration (NNSA)

    because we were not stabilizing these, and I needed to put solar panels on all six sides. ... Seitz closes up the cubesat. A look at the cubesat's solar panels. J.P. Martinez and Dan ...

  9. Materials Data on BaBi2O5 (SG:123) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2015-04-10

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  10. Fact #872: May 11, 2015 Study Finds More than 60% of Millennials and Generation Xers Use the Internet to Find a Car Dealer While Less than Half of Baby Boomers Did

    Broader source: Energy.gov [DOE]

    According to an AutoTrader-commissioned study of people who purchased vehicles within the past 12 months, the Internet is the source most used when finding a car dealer. However, the study revealed...

  11. 1 of 8

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (5265) to request a copy. Having a baby (normal delivery) Managing type 2 diabetes (routine maintenance of a well-controlled condition) About these Coverage Examples:...

  12. 1 of 8

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    page.--- 7 of 8 Having a baby (normal delivery) Managing type 2 diabetes (routine maintenance of a well-controlled condition) About these Coverage Examples:...

  13. Ultrasonic Characterization of Wastes | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ultrasonic Characterization of Wastes It's commonplace for seeing babies in utero, fish underwater and submarines in the ocean, but now sonar technology will be giving DOE an image...

  14. Fermilab Today

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    nature, animal, reptile, turtle, snapping turtle A baby snapping turtle walks on the pedestrian path leading to Wilson Hall. Photo: Chris Sheppard, CCD In the News 4,850 feet...

  15. Fact #872: May 11, 2015 Study Finds More than 60% of Millennials...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Car Dealer While Less than Half of Baby Boomers Did - Dataset Fact 872: May 11, 2015 Study Finds More than 60% of Millennials and Generation Xers Use the Internet to Find a Car ...

  16. Fact #872: May 11, 2015 Study Finds More than 60% of Millennials...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    the Internet to Find a Car Dealer While Less than Half of Baby Boomers Did Fact 872: May 11, 2015 Study Finds More than 60% of Millennials and Generation Xers Use the Internet to ...

  17. 1 of 8

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1-877-878-LANL (5265) to request a copy. Having a baby (normal delivery) Managing type 2 diabetes (routine maintenance of a well-controlled condition) About these Coverage...

  18. Stephen Hawking

    ScienceCinema (OSTI)

    None

    2011-04-25

    Le grand astrophysicien S.Hawking, né le 08-01-1942 à Oxford, parle de "baby universes" et la gravitation et répond aux questions.

  19. US ITER | Media Corner

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    From Snowballs in Hell to Burping the Baby From "Snowballs in Hell" to "Burping the Baby" US Researchers Parse Complex Plasma Issues -Agatha Bardoel Published October 31, 2011 Vacuum Pressure Impregnation Vessel A large (+16 mm) pellet is undergoing testing for ITER disruption mitigation. In this photo, the pellet, on the left, is exiting the guide tube just before hitting a simple target plate. It will shatter once it hits the plate. (Photo: Combs, ORNL) Heated to extreme

  20. Contrasting impacts of localised versus catastrophic oil spills in coastal wetlands

    SciTech Connect (OSTI)

    Burns, K.A.; Codi, S.

    1996-12-31

    A localised oil spill was observed on the wetland marshes bordering a tidal creek near Cairns, Queensland in January 1994. Pollution and conservation issues are of paramount public concern in this region which boarders World Heritage Areas of coral reefs and coastal habitats. Local residents observed oil being dumped from a truck which was contracted to of oil the surface of the roads in the contiguous sugar cane farm for dust control. During this incident several truckloads of mixed waste oil were dumped onto a short section of road and into the wetlands. The oil contaminated a band of marsh 15-30 m wide along approximately 200 m of road. Impacted marsh included Melaleuca forest on the high side of the road and intertidal mangroves on the seaward side. The Queensland Department of Environment (QDE) initiated an impact assessment and directed the trucking company to clean up impacted areas. The extent of damage to wetlands from oil spills is related to the amount and type of oil spilled and the sensitivity of the habitats oiled. QDE asked the Australian Institute of Marine Sciences to assist with their study on the fate of the oil in this localised spill. The initial levels of petroleum hydrocarbons in surface sediments reached 17% of the dry weight in heavily impacted areas. Thus levels were similar to those reached after the catastrophic oil spill in Panama. Clean up efforts and natural dissipation processes reduced sediment hydrocarbon loads to nonacutely toxic levels in only 1.5 years in the intertidal mangroves. High levels remain in the Melaleuca sediments. We used internal molecular markers to detail hydrocarbon dissipation vs degradation. This study provides a contrast between impacts of localised versus catastrophic oil spills in deep mud coastal habitats.

  1. How Long Has Grandpa Been Dead and Other Forensic Mysteries

    ScienceCinema (OSTI)

    Baden, Michael [MD, New York Police, New York, New York, United States

    2009-09-01

    Was the baby born alive? Can a child's brain really be shaken hard enough to cause death? Was the body dead before going into the water? Does a lightening strike cause any unique changes in the body? Why are hair and maggots becoming so important to the forensic scientist? Let's talk.

  2. DOE Research and Development Accomplishments Snapshots

    Office of Scientific and Technical Information (OSTI)

    Snapshots Battery Power * Communications * Electronics * Energy * Elements * Environment * Health and Medicine Life: Babies and Archaea * Neutrinos * Quarks * Technology and Inventions * WWII/Manhattan Project This page contains links to Snapshots of Department of Energy (DOE) Research and Development (R&D) Accomplishments. These Snapshots are the outcomes of past R&D that have had economic impact or have improved people’s lives, or which promise to do so. They are quick pictures,

  3. Science On Saturday Archive | Princeton Plasma Physics Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Upcoming Events Events Calendar Colloquia Archive Science On Saturday Archive Research Education Organization Contact Us Upcoming Events Events Calendar Colloquia Archive Science On Saturday Archive Science On Saturday Archive EXTENDED! Science on Saturday: Using Physics and Chemistry to Understand the Genome March 19, 2016 Professor Mary Jo Ondrechen Northeastern University Science on Saturday: Taking the Universe's Baby Picture March 12, 2016 Professor David Spergel Princeton University

  4. Human radiation studies: Remembering the early years. Oral history of radiologist Earl R. Miller, M.D., August 9 and 17, 1994

    SciTech Connect (OSTI)

    1995-06-01

    Dr. Earl R. Miller was interviewed by representatives of US DOE Office of Human Radiation Research (OHRE). The interview covers Dr. Miller`s involvement with the Manhattan Engineer District, with total body irradiation, and heavy-ion therapy. Dr. Miller`s remembrances include wartime work on radiation exposure, Joe Hamilton, Neutron Therapy research, means of obtaining isotopes, consent forms, infinite laminograms, invention of a baby holder to alleviate exposure of radiological technicians in diagnostic procedures involving infants, and several personages.

  5. LANL: AOT & LANSCE The Pulse

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    SPECIAL ISSUE: LANSCE Central Control Room The constant murmur of talking and sound of button pushing seeps through the faded baby blue metal double doors to the Central Control Room of the Los Alamos Neutron Science Center (LANSCE). Inside, at the center of the dimly lit room sits a sleek, elongated, horseshoe-shaped console where four operators on the Accelerator and Operations Technology-Accelerator Operations Beam Delivery Team focus on their tasks. 2 From Alex's Desk 3 Power providers 4

  6. Microsoft Word - D. Spergel.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2 March 2016 Taking the Baby Picture of the Universe Dr. David Spergel Professor of Astronomy & Chair of he Department of Astrophysical Sciences Princeton University Princeton, NJ ABSTRACT: Observations of the microwave background, the left-over heat from the big bang, are snap-shots of the universe only three hundred thousand years after the big bang. These observations have answered many of the questions that have driven cosmology for the past few decades: How old is the universe? What is

  7. Measuring Complementary Electronic Structure Properties of both Deposited and Gas Phase Clusters using STM, UPS, and PES: Size-Selected Clusters on Surfaces

    SciTech Connect (OSTI)

    Bowen, Kit H.

    2014-03-05

    In this project, we studied size-selected cluster interactions with surfaces, with other clusters on surfaces, and with external stimuli. These studies focused on mobility as a function of cluster size, surface morphologies as a function of composition and coverage, ion-induced modification and reactivity of clusters as a function of composition, the structural evolution of cluster cuboids culminating in the characterization of theoretically-predicted baby crystal clusters, and unusual fractal pattern formation due to deposition.

  8. SU-E-J-145: Geometric Uncertainty in CBCT Extrapolation for Head and Neck Adaptive Radiotherapy

    SciTech Connect (OSTI)

    Liu, C; Kumarasiri, A; Chetvertkov, M; Gordon, J; Chetty, I; Siddiqui, F; Kim, J

    2014-06-01

    Purpose: One primary limitation of using CBCT images for H'N adaptive radiotherapy (ART) is the limited field of view (FOV) range. We propose a method to extrapolate the CBCT by using a deformed planning CT for the dose of the day calculations. The aim was to estimate the geometric uncertainty of our extrapolation method. Methods: Ten H'N patients, each with a planning CT (CT1) and a subsequent CT (CT2) taken, were selected. Furthermore, a small FOV CBCT (CT2short) was synthetically created by cropping CT2 to the size of a CBCT image. Then, an extrapolated CBCT (CBCTextrp) was generated by deformably registering CT1 to CT2short and resampling with a wider FOV (42mm more from the CT2short borders), where CT1 is deformed through translation, rigid, affine, and b-spline transformations in order. The geometric error is measured as the distance map ||DVF|| produced by a deformable registration between CBCTextrp and CT2. Mean errors were calculated as a function of the distance away from the CBCT borders. The quality of all the registrations was visually verified. Results: Results were collected based on the average numbers from 10 patients. The extrapolation error increased linearly as a function of the distance (at a rate of 0.7mm per 1 cm) away from the CBCT borders in the S/I direction. The errors (??) at the superior and inferior boarders were 0.8 0.5mm and 3.0 1.5mm respectively, and increased to 2.7 2.2mm and 5.9 1.9mm at 4.2cm away. The mean error within CBCT borders was 1.16 0.54mm . The overall errors within 4.2cm error expansion were 2.0 1.2mm (sup) and 4.5 1.6mm (inf). Conclusion: The overall error in inf direction is larger due to more large unpredictable deformations in the chest. The error introduced by extrapolation is plan dependent. The mean error in the expanded region can be large, and must be considered during implementation. This work is supported in part by Varian Medical Systems, Palo Alto, CA.

  9. High-dose radioiodine treatment for differentiated thyroid carcinoma is not associated with change in female fertility or any genetic risk to the offspring

    SciTech Connect (OSTI)

    Bal, Chandrasekhar . E-mail: csbal@hotmail.com; Kumar, Ajay; Tripathi, Madhavi; Chandrashekar, Narayana; Phom, Hentok; Murali, Nadig R.; Chandra, Prem; Pant, Gauri S.

    2005-10-01

    Background: We tried to evaluate the female fertility and genetic risk to the offspring from the exposure to high-dose {sup 131}I by assessing the pregnancy outcomes and health status of the children of female patients with differentiated thyroid cancer who had received therapeutic doses of {sup 131}I. Materials and Methods: From 1967 to 2002, a total of 1,282 women had been treated with {sup 131}I. Of these patients, 692 (54%) were in the reproductive age group (18-45 years). Forty women had a total of 50 pregnancies after high-dose {sup 131}I. Age at presentation ranged from 16 to 36 years (mean, 23 {+-} 4 years). Histopathology was papillary thyroid cancer in 32 cases and follicular thyroid cancer in 8 cases. Results: Single high-dose therapy was given in 30 cases, 2 doses were given in 7 cases, 3 doses were given in 2 cases, and four doses were given in 1 case in which lung metastases had occurred. In 37 patients (92%), disease was successfully ablated before pregnancy. Ovarian absorbed-radiation dose calculated by the MIRD method ranged from 3.5 to 60 cGy (mean, 12 {+-} 11 cGy). The interval between {sup 131}I therapy and pregnancy varied from 7 to 120 months (37.4 {+-} 28.2 months). Three spontaneous abortions occurred in 2 women. Forty-seven babies (20 females and 27 males) were born. Forty-four babies were healthy with normal birth weight and normal developmental milestones. Twenty women delivered their first baby after {sup 131}I therapy. The youngest child in our series is 11 months of age, and the oldest is 8.5 years of age. Conclusions: Female fertility is not affected by high-dose radioiodine treatment, and the therapy does not appear to be associated with any genetic risks to the offspring.

  10. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Preparing the Lab for workforce needs May 2, 2016 Our Laboratory employs more than 10,000 people-one fifth of who are eligible to embark on a well-deserved retirement in the next four years (congratulations, baby boomers, you made it!). But what's a Laboratory to do when its workforce of 30-some years trades chemistry for crosswords? Hire new people, of course, which is exactly what Laboratory Director Charlie McMillan told community leaders at a recent meeting. "You're looking at something

  11. INSIDE: ITER Site Progress

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Industry Monaco/ITER Postdoctoral Fellowship Program Upcoming Events US Researchers Parse Complex Plasma Issues From "Snowballs in Hell" to "Burping the Baby" - by Agatha Bardoel The design, testing, and manufacture of a pellet injection system is one of the key contributions of the United States to ITER. Physicist David Rasmussen serves as the lead for US ITER's fueling team and as a group leader in the Fusion Energy Division at Oak Ridge National Laboratory. He points out

  12. The Modern Grid Initiative is a DOE-funded project managed by the National Energy Technology Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    McAdams Theory of grid modernization. This is final in a series of discussions on how different mindsets look at grid modernization. With four generation "X" and "Y" children growing up in our house over the last 25 years, we had the opportunity to begin to understand how this next generation of consumers, leaders, designers, and builders view the electric system. Briefly, generation X (GenX) are those who have grown up in the shadow of the Baby Boomers and are roughly 25 to

  13. The time it takes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    time it takes... 180 days 254 days 280 days 365 days to grow 3 inches of hair to travel to and land on Mars for a human baby to develop to term for one Earth-year 12 1 2 3 4 5 6 7 8 9 10 11 39 minutes longer than a day on Earth. A day on Mars lasts This year's high school graduates have never known a world in which there were no robotic vehicles cruising around on Mars. There are presently six active spacecraft orbiting around or driving on Mars. Los Alamos scientists helped to develop the

  14. DOE Worklife4You Program | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Services » Benefits » Wellness Programs » DOE Worklife4You Program DOE Worklife4You Program If you are struggling to find the time to manage important events in your life while meeting the demands of your job, let the DOE Headquarters WorkLife4You program work for you! Major life events such as having a baby, going to college, caring for an aging loved one, or planning for retirement can be overwhelming because they compete for your valuable time at work and at home. To offer support, DOE

  15. Bandwidth Study U.S. Titanium Manufacturing, Draft | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Joshua DeLung What does this mean for me? The Baltimore Green and Healthy Homes Initiative program, made possible with Recovery Act dollars, provides comprehensive health, safety, and energy efficiency upgrades to low-income families around the city. Lekquan Young rushed her 8-month-old son to the hospital when she noticed his chest looked sunken as he breathed. The doctor told her that her baby son had asthma. Today, her son is 8 years old and has suffered frequent asthma attacks at home.

  16. Illicit Trafficking in Radiological and Nuclear Materials. Lack of Regulations and Attainable Disposal for Radioactive Materials Make Them More Vulnerable than Nuclear Materials

    SciTech Connect (OSTI)

    Balatsky, G.I.; Severe, W.R.; Leonard, L.

    2007-07-01

    Illicit trafficking in nuclear and radioactive materials is far from a new issue. Reports of nuclear materials offered for sale as well as mythical materials such as red mercury date back to the 1960's. While such reports were primarily scams, it illustrates the fact that from an early date there were criminal elements willing to sell nuclear materials, albeit mythical ones, to turn a quick profit. In that same time frame, information related to lost and abandoned radioactive sources began to be reported. Unlike reports on nuclear material of that era, these reports on abandoned sources were based in fact - occasionally associated with resulting injury and death. With the collapse of the Former Soviet Union, illicit trafficking turned from a relatively unnoticed issue to one of global concern. Reports of unsecured nuclear and radiological material in the states of the Former Soviet Union, along with actual seizures of such material in transit, gave the clear message that illicit trafficking was now a real and urgent problem. In 1995, the IAEA established an Illicit Trafficking Data Base to keep track of confirmed instances. Illicit Trafficking is deemed to include not only radioactive materials that have been offered for sale or crossed international boarders, but also such materials that are no longer under appropriate regulatory control. As an outcome of 9/11, the United States took a closer look at illicit nuclear trafficking as well as a reassessment of the safety and security of nuclear and other radioactive materials both in the United States and Globally. This reassessment launched heightened controls and security domestically and increased our efforts internationally to prevent illicit nuclear trafficking. This reassessment also brought about the Global Threat Reduction Initiative which aims to further reduce the threats of weapons usable nuclear materials as well those of radioactive sealed sources. This paper will focus on the issues related to a subset of the materials involved in illicit trafficking in nuclear and radioactive materials, that of radioactive sealed sources. The focus on radioactive sealed sources is based on our belief that insufficient attention has been paid to trafficking incidents involving such sources which constitute the majority of trafficking cases. According to the IAEA's Illicit Trafficking Data Base, as of December 31 2005 there were 827 confirmed cases reporting by the participating states, including 250 incidents (or 30%) involved nuclear and other radioactive materials and 566 (or 68%) involved other radioactive materials, mostly radioactive sources, and radioactively contaminated materials. Experts in the Lugar Survey on Proliferation Threat and Response (June 2005) agreed that an attack with a Radiological Dispersion Device (RDD) was the most probable form of nuclear terrorism the world could expect over the next decade. At the same time radiological materials are used in wide a variety of applications, located in virtually every country and in general, radiological materials are far easier to access than nuclear materials. It has become increasingly obvious that the lack of a cradle-to-grave approach for sealed radioactive sources that have reached the end of their useful life is the main reason that sources are abandoned. It appears that the questions will ultimately become whether industry will impose additional regulations upon itself and become self-regulating with respect to repatriating radioactive material at the end of service life, or whether national authorities at some point will take actions and regulate the industry. Argentina, which is one of the most advanced countries regarding control of radiological sources adopted additional measures to safeguard its radiological materials to a level comparable to that proscribed for nuclear materials. This approach, while highly successful, has led to some minor unforeseen consequences, namely insufficient funds to implement all regulations in full and a lack of inspectors and appropriate equipment to assure compliance This

  17. Phenomenological loop quantum geometry of the Schwarzschild black hole

    SciTech Connect (OSTI)

    Chiou, D.-W.

    2008-09-15

    The interior of a Schwarzschild black hole is investigated at the level of phenomenological dynamics with the discreteness corrections of loop quantum geometry implemented in two different improved quantization schemes. In one scheme, the classical black hole singularity is resolved by the quantum bounce, which bridges the black hole interior with a white hole interior. In the other scheme, the classical singularity is resolved and the event horizon is also diffused by the quantum bounce. Jumping over the quantum bounce, the black hole gives birth to a baby black hole with a much smaller mass. This lineage continues as each classical black hole brings forth its own descendant in the consecutive classical cycle, giving the whole extended spacetime fractal structure, until the solution eventually descends into the deep Planck regime, signaling a breakdown of the semiclassical description. The issues of scaling symmetry and no-hair theorem are also discussed.

  18. A knowledge continuity management program for the energy, infrastructure and knowledge systems center, Sandia National Laboratories.

    SciTech Connect (OSTI)

    Menicucci, David F.

    2006-07-01

    A growing recognition exists in companies worldwide that, when employees leave, they take with them valuable knowledge that is difficult and expensive to recreate. The concern is now particularly acute as the large ''baby boomer'' generation is reaching retirement age. A new field of science, Knowledge Continuity Management (KCM), is designed to capture and catalog the acquired knowledge and wisdom from experience of these employees before they leave. The KCM concept is in the final stages of being adopted by the Energy, Infrastructure, and Knowledge Systems Center and a program is being applied that should produce significant annual cost savings. This report discusses how the Center can use KCM to mitigate knowledge loss from employee departures, including a concise description of a proposed plan tailored to the Center's specific needs and resources.

  19. Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins.

    SciTech Connect (OSTI)

    Jenko, Kathryn; Zhang, Yanfeng; Kostenko, Yulia; Fan, Yongfeng; Garcia-Rodriguez, Consuelo; Lou, Jianlong; Marks, James D.; Varnum, Susan M.

    2014-10-21

    Plant and microbial toxins are considered bioterrorism threat agents because of their extreme toxicity and/or ease of availability. Additionally, some of these toxins are increasingly responsible for accidental food poisonings. The current study utilized an ELISA-based protein antibody microarray for the multiplexed detection of ten biothreat toxins, botulinum neurotoxins (BoNT) A, B, C, D, E, F, ricin, shiga toxins 1 and 2 (Stx), and staphylococcus enterotoxin B (SEB), in buffer and complex biological matrices. The multiplexed assay displayed a sensitivity of 1.3 pg/mL (BoNT/A, BoNT/B, SEB, Stx-1 and Stx-2), 3.3 pg/mL (BoNT/C, BoNT/E, BoNT/F) and 8.2 pg/mL (BoNT/D, ricin). All assays demonstrated high accuracy (75-120 percent recovery) and reproducibility (most coefficients of variation < 20%). Quantification curves for the ten toxins were also evaluated in clinical samples (serum, plasma, nasal fluid, saliva, stool, and urine) and environmental samples (apple juice, milk and baby food) with overall minimal matrix effects. The multiplex assays were highly specific, with little crossreactivity observed between the selected toxin antibodies. The results demonstrate a multiplex microarray that improves current immunoassay sensitivity for biological warfare agents in buffer, clinical, and environmental samples.

  20. Fast methods for analysis of neurotransmitters from single cell and monitoring their releases in central nervous system by capillary electrophoresis, fluorescence microscopy and luminescence imaging

    SciTech Connect (OSTI)

    Wang, Ziqiang

    1999-12-10

    Fast methods for separation and detection of important neurotransmitters and the releases in central nervous system (CNS) were developed. Enzyme based immunoassay combined with capillary electrophoresis was used to analyze the contents of amino acid neurotransmitters from single neuron cells. The release of amino acid neurotransmitters from neuron cultures was monitored by laser induced fluorescence imaging method. The release and signal transduction of adenosine triphosphate (ATP) in CNS was studied with sensitive luminescence imaging method. A new dual-enzyme on-column reaction method combined with capillary electrophoresis has been developed for determining the glutamate content in single cells. Detection was based on monitoring the laser-induced fluorescence of the reaction product NADH, and the measured fluorescence intensity was related to the concentration of glutamate in each cell. The detection limit of glutamate is down to 10{sup {minus}8} M level, which is 1 order of magnitude lower than the previously reported detection limit based on similar detection methods. The mass detection limit of a few attomoles is far superior to that of any other reports. Selectivity for glutamate is excellent over most of amino acids. The glutamate content in single human erythrocyte and baby rat brain neurons were determined with this method and results agreed well with literature values.

  1. Removal of Radiocesium from Food by Processing: Data Collected after the Fukushima Daiichi Nuclear Power Plant Accident - 13167

    SciTech Connect (OSTI)

    Uchida, Shigeo; Tagami, Keiko

    2013-07-01

    Removal of radiocesium from food by processing is of great concern following the accident of TEPCO's Fukushima Daiichi Nuclear Power Plant accident. Foods in markets are monitored and recent monitoring results have shown that almost all food materials were under the standard limit concentration levels for radiocesium (Cs-134+137), that is, 100 Bq kg{sup -1} in raw foods, 50 Bq kg{sup -1} in baby foods, and 10 Bq kg{sup -1} in drinking water; those food materials above the limit cannot be sold. However, one of the most frequently asked questions from the public is how much radiocesium in food would be removed by processing. Hence, information about radioactivity removal by processing of food crops native to Japan is actively sought by consumers. In this study, the food processing retention factor, F{sub r}, which is expressed as total activity in processed food divided by total activity in raw food, is reported for various types of corps. For white rice at a typical polishing yield of 90-92% from brown rice, the F{sub r} value range was 0.42-0.47. For leafy vegetable (indirect contamination), the average F{sub r} values were 0.92 (range: 0.27-1.2) after washing and 0.55 (range: 0.22-0.93) after washing and boiling. The data for some fruits are also reported. (authors)

  2. DIFFERENTIAL SENSITIVITY OF MALE GERM CELLS TO MAINSTREAM AND SIDESTREAM TOBACCO SMOKE IN THE MOUSE

    SciTech Connect (OSTI)

    Polyzos, Aris; Schmid, Thomas Ernst; Pina-Guzman, Belem; Quintanilla-Vega, Betzabet; Marchetti, Francesco

    2009-03-13

    Cigarette smoking in men has been associated with increased chromosomal abnormalities in sperm and with increased risks for spontaneous abortions, birth defects and neonatal death. Little is known, however, about the reproductive consequences of paternal exposure to second-hand smoke. We used a mouse model to investigate the effects of paternal exposure to sidestream (SS) smoke, the main constituent of second-hand smoke, on the genetic integrity and function of sperm, and to determine whether male germ cells were equally sensitive to mainstream (MS) and SS smoke. A series of sperm DNA quality and reproductive endpoints were investigated after exposing male mice for two weeks to MS or SS smoke. Our results indicated that: (i) only SS smoke significantly affected sperm motility; (ii) only MS smoke induced DNA strand breaks in sperm; (iii) both MS and SS smoke increased sperm chromatin structure abnormalities; and (iv) MS smoke affected both fertilization and the rate of early embryonic development, while SS smoke affected fertilization only. These results show that MS and SS smoke have differential effects on the genetic integrity and function of sperm and provide further evidence that male exposure to second-hand smoke, as well as direct cigarette smoke, may diminish a couple's chance for a successful pregnancy and the birth of a healthy baby.

  3. Fertility and Pregnancy Outcome After Abdominal Irradiation That Included or Excluded the Pelvis in Childhood Tumor Survivors

    SciTech Connect (OSTI)

    Sudour, Helene; Chastagner, Pascal; Claude, Line; Desandes, Emmanuel; Klein, Marc; Carrie, Christian; Bernier, Valerie

    2010-03-01

    Purpose: To evaluate fertility after abdominal and/or pelvic irradiation in long-term female survivors. Methods and Materials: Puberty and pregnancy outcome were analyzed in female survivors of childhood cancer (aged <18 years) treated with abdominal and/or pelvic radiotherapy (RT) at one of two French centers (Nancy and Lyon) between 1975 and 2004. Data were obtained from medical records and questionnaires sent to the women. Results: A total of 84 patients who had received abdominal and/or pelvic RT during childhood and were alive and aged more than 18 years at the time of the study made up the study population. Of the 57 female survivors treated with abdominal RT that excluded the pelvis, 52 (91%) progressed normally through puberty and 23 (40%) had at least one recorded pregnancy. Of the 27 patients treated with pelvic RT, only 10 (37%) progressed normally through puberty and 5 (19%) had at least one recorded pregnancy. Twenty-two women (seventeen of whom were treated with pelvic RT) had certain subfertility. A total of 50 births occurred in 28 women, with one baby dying at birth; one miscarriage also occurred. There was a high prevalence of prematurity and low birth weight but not of congenital malformations. Conclusions: Fertility can be preserved in patients who undergo abdominal RT that excludes the pelvis, taking into account the other treatments (e.g., chemotherapy with alkylating agents) are taken into account. When RT includes the pelvis, fertility is frequently impaired and women can have difficulty conceiving. Nevertheless, pregnancies can occur in some of these women. The most important factor that endangers a successful pregnancy after RT is the total dose received by the ovaries and uterus. This radiation dose has to be systematically recorded to improve our ability to follow up patients.

  4. STEm Minority Graduate Program

    SciTech Connect (OSTI)

    Kaen E. Nicholas

    2012-09-20

    ABSTRACT The state of science, technology, engineering and math (STEM) education in the United States has seen some unfavorable assessments over the past decade. In early February, 2010 the House of Representatives heard testimony on undergraduate and graduate education. The message from the panel, which included experts from academia, STEM-based industries, and the National Science Foundation (NSF) was dire and required an urgent response. The experts along with the committee???¢????????s chairperson, U. S. Representative Daniel Lipinski (D-IL) cited that the complexity of Science, Technology, Engineering, and Mathematics applications and coursework and the methodology utilized to teach these subjects are forcing students out of these disciplines. As the National Academies described in its 2007 report Rising Above the Gathering Storm, successful STEM education is not just an academic pursuit???¢????????it???¢????????s a necessity for competing in the knowledge-based economy that the United States had a key role in creating. The potential for action is being made available again as the America COMPETES Act of 2007 is up for reauthorization. Its initial focus was on STEM education at the K-12 levels, but efforts at the undergraduate and graduate levels are needed to retain students to fill the jobs left vacant as baby boomers retire. The Educational Advancement Alliance, Inc. (EAA) has for two decades created programs that have not only addressed the issues of ensuring that students are aptly prepared for college but have focused its efforts over the past decade on increasing the number of students who pursue degrees in STEM disciplines. For the EAA, the introduction of the wonders of science begins at the elementary and middle school level via the Learning Lab, a state-of-the-art mobile science laboratory that visits students in grades 4-6 at the various schools throughout Philadelphia and The Math/Tech Academy which meets on Saturdays for students in grades 5-7. For the past two years the EAA has assisted college graduates in their quest to attain advanced degrees in STEM by providing fellowships. The EAA continued this effort by recruiting and providing fellowships to students who aspired to continue their education at the graduate level. The fellowships provided funding for tuition, fees, books, technology, and stipends to assist with room, board, and living expenses during the academic year and salary, transportation, and living expenses to those students who secured internships with the Department of Energy. Additionally the EAA designed and implemented needed support systems to ensure successful completion of the Masters degree programs, including but not limited to membership in professional associations, attendance at industry and academic conferences, and professional development workshops, and tutorial assistance if needed. This program assisted over 80 students directly and society-at-large by helping to educate and develop future physicists, engineers, biostatisticians, and researchers who will have the necessary skillsets to fill the increasing numbers of positions that require such expertise.

  5. Final Technical Report - DE-EE0003542

    SciTech Connect (OSTI)

    Haley, James D

    2013-03-31

    Wind has provided energy for thousands of years: some of the earliest windmill engineering designs date back to ancient Babylonia and India where wind would be used as a source of irrigation. Today, wind is the quickest growing resource in Americas expanding energy infrastructure. However, to continue to positively diversify Americas energy portfolio and further reduce the countrys reliance of foreign oil, the industry must grow substantially over the next two decades in both turbine installations and skilled industrial manpower to support. The wind sector is still an emergent industry requiring maturation and development of its labor force: dedicated training is needed to provide the hard and soft skills to support the increasingly complex wind turbine generators as the technology evolves. Furthermore, the American workforce is facing a steep decline in available labor resources as the baby boomer generation enters retirement age. It is therefore vital that a process is quickly created for supporting the next generation of wind technicians. However, the manpower growth must incorporate three key components. First, the safety and technical training curriculum must be standardized across the industry - current wind educational programs are disparate and dedicated standardization programs must be further refined and implemented. Second, it is essential that the wind sector avoid disrupting other energy production industries by cannibalizing workers, which would indirectly affect the rest of Americas energy portfolio. The future wind workforce must be created organically utilizing either young people entering the workforce or train personnel emerging from careers outside of energy production. Third, the training must be quick and efficient as large amounts of wind turbines are being erected each year and this growth is expected to continue until at least 2035. One source that matches these three requirements is personnel transitioning from military service to the civilian sector. Utilizing the labor pool of transitioning military personnel and a dedicated training program specifically tailored to military hard and soft skills, the wind workforce can rapidly expand with highly skilled personnel. A tailored training program also provides career opportunities to an underutilized labor force as the personnel return from active military duty. This projects goal was to create a Wind Workforce Development Program that streamlines the wind technician training process using industry-leading safety programs and building on existing military experience. The approach used was to gather data from the wind industry, develop the curriculum and test the process to ensure it provides adequate training to equip the technicians as they transition from the military into wind. The platform for the curriculum development is called Personal Qualification Standards (PQS), which is based on the program of the same name from the United States Navy. Not only would the program provide multiple delivery methods of training (including classroom, computer-based training and on-the-job training), but it also is a familiar style of training to many military men and women. By incorporating a familiar method of training, it encourages active participation in the training and reduces the time for personnel to grasp the concept and flow of the training requirements. The program was tested for thoroughness, schedule and efficacy using a 5-person pilot phase during the last two years. The results of the training were a reduction in time to complete training and increased customer satisfaction on client project sites. However, there were obstacles that surfaced and required adaptation throughout the project including method of delivery, curriculum development and project schedules and are discussed in detail throughout the report. There are several key recommendations in the report that discuss additional training infrastructure, scalability within additional alternative energy markets and organizational certification through standardization committees.