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Sample records for association rfa promotes

  1. Microsoft PowerPoint - RFA_EIA_conference_2009.ppt

    U.S. Energy Information Administration (EIA) Indexed Site

    America's Ethanol Industry Our Energy Future Starts at Home About the RFA * The Renewable Fuels Association (RFA) promotes policies, regulations and research and development initiatives that will lead to the increased production and use of fuel ethanol. * RFA membership includes a broad cross- section of businesses, individuals and organizations dedicated to the expansion of the U.S. fuel ethanol industry. Ethanol Facts 2008 Production / 2009 Estimate 9.2 billion gallons / 10.5 billion gallons

  2. RFA-14-0001 - In the Matter of Commonwealth Oil Refining Company...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    RFA-14-0001 - In the Matter of Commonwealth Oil Refining Company, Inc.Commonwealth of Puerto Rico RFA-14-0001 - In the Matter of Commonwealth Oil Refining Company, Inc....

  3. RCRA Facilities Assessment (RFA)---Oak Ridge National Laboratory

    SciTech Connect (OSTI)

    Not Available

    1987-03-01

    US Department of Energy (DOE) facilities are required to be in full compliance with all federal and state regulations. In response to this requirement, the Oak Ridge National Laboratory (ORNL) has established a Remedial Action Program (RAP) to provide comprehensive management of areas where past and current research, development, and waste management activities have resulted in residual contamination of facilities or the environment. This report presents the RCRA Facility Assessment (RFA) required to meet the requirements of RCRA Section 3004(u). Included in the RFA are (1) a listing of all sites identified at ORNL that could be considered sources of releases or potential releases; (2) background information on each of these sites, including location, type, size, period of operation, current operational status, and information on observed or potential releases (as required in Section II.A.1 of the RCRA permit); (3) analytical results obtained from preliminary surveys conducted to verify the presence or absence of releases from some of the sites; and (4) ORNL`s assessment of the need for further remedial attention.

  4. RCRA Facilities Assessment (RFA)---Oak Ridge National Laboratory

    SciTech Connect (OSTI)

    Not Available

    1987-03-01

    US Department of Energy (DOE) facilities are required to be in full compliance with all federal and state regulations. In response to this requirement, the Oak Ridge National Laboratory (ORNL) has established a Remedial Action Program (RAP) to provide comprehensive management of areas where past and current research, development, and waste management activities have resulted in residual contamination of facilities or the environment. This report presents the RCRA Facility Assessment (RFA) required to meet the requirements of RCRA Section 3004(u). Included in the RFA are (1) a listing of all sites identified at ORNL that could be considered sources of releases or potential releases; (2) background information on each of these sites, including location, type, size, period of operation, current operational status, and information on observed or potential releases (as required in Section II.A.1 of the RCRA permit); (3) analytical results obtained from preliminary surveys conducted to verify the presence or absence of releases from some of the sites; and (4) ORNL's assessment of the need for further remedial attention.

  5. ORISE: Iddins promoted to associate director of Radiation Emergency

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Assistance Center/Training Site Iddins promoted to associate director of Radiation Emergency Assistance Center/Training Site New director brings more than 20 years of medical expertise FOR IMMEDIATE RELEASE Oct. 14, 2014 FY15-01 Dr. Carol Iddins Dr. Carol Iddins (Click for high resolution version) OAK RIDGE, Tenn.-Dr. Carol J. Iddins has been named associate director of the Radiation Emergency Assistance Center/Training Site (REAC/TS). In this position, Iddins will provide management and

  6. RCRA Facilities Assessment (RFA) Oak Ridge National Laboratory addendum August 25, 1987

    SciTech Connect (OSTI)

    Not Available

    1987-08-01

    The RCRA Facilities Assessment (RFA) report identified approximately 250 Solid Waste Management Units (SWMUs) that were grouped into 20 Waste Area Groupings (WAGs) at Oak Ridge National Laboratory. Identification of each SWMU included information as to location, type, size, dates of operation, type of waste handled, and evidence of releases. Preliminary sampling studies were performed around each WAG to determine if there was evidence of releases beyond its perimeter. Analytical results from the surveys and historical information were the basis for recommendations concerning further actions for each WAG. Remedial investigations (RIs) were recommended for WAGs 1--10 and 17; for WAGs 14, 16, 18, and 20, it was suggested that they be removed from further consideration for remedial action. For the remaining WAGs (11, 12, 13, 15, and 19) the evidence concerning the possible release of contaminants was inconclusive and additional sampling was recommended. The purpose of this Addendum is to report the analytical data obtained from the additional surveys, to make recommendations concerning future remedial actions within these WAGs, and to provide descriptive information for additional sites listed in Table 1.2 of the RFA. Since information concerning the rationale for identifying releases, the sampling survey methodology, and background information for each WAG is presented in the RFA, it is not repeated in this Addendum.

  7. Image-Guided Radiofrequency Ablation (RFA) of Unresectable Hepatic Tumors Using a Triple-Spiral-Shaped Electrode Needle: Initial Experience in 34 Patients

    SciTech Connect (OSTI)

    Thanos, Loukas; Poulou, Loukia S.; Ziakas, Panayiotis D.; Kelekis, Alexis D.; Pomoni, Maria; Kelekis, Dimitrios A.

    2010-02-15

    We evaluated the safety and efficacy of image-guided radiofrequency ablation (RFA) using a triple-spiral-shaped electrode needle for unresectable primary or metastatic hepatic tumors. Thirty-four patients with 46 index tumors were treated. Ablation zone, morbidity, and complications were assessed. The lesions were completely ablated with an ablative margin of about 1 cm. Five patients (14.7%) with a lesion larger than 4.5 cm had local tumor progression after 1 month and were retreated. Hemothorax, as a major complication, occurred in 1 of 34 patients (3.0%) or 1 of 46 lesions ablated (2.2%). RFA using this new electrode needle can be effective in the treatment of large unresectable hepatic tumors.

  8. Transcriptional Responses to Sucrose Mimic the Plant-Associated Life Style of the Plant Growth Promoting Endophyte Enterobacter sp. 638

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Taghavi, Safiyh; Wu, Xiao; Ouyang, Liming; Zhang, Yian Biao; Stadler, Andrea; McCorkle, Sean; Zhu, Wei; Maslov, Sergei; van der Lelie, Daniel

    2015-01-21

    Growth in sucrose medium was previously found to trigger the expression of functions involved in the plant associated life style of the endophytic bacterium Enterobacter sp. 638. Therefore, comparative transcriptome analysis between cultures grown in sucrose or lactate medium was used to gain insights in the expression levels of bacterial functions involved in the endophytic life style of strain 638. Growth on sucrose as a carbon source resulted in major changes in cell physiology, including a shift from a planktonic life style to the formation of bacterial aggregates. This shift was accompanied by a decrease in transcription of genes involvedmore » in motility (e.g. flagella biosynthesis) and an increase in the transcription of genes involved in colonization, adhesion and biofilm formation. The transcription levels of functions previously suggested as being involved in endophytic behavior and functions responsible for plant growth promoting properties, including the synthesis of indole-acetic acid, acetoin and 2,3-butanediol, also increased significantly for cultures grown in sucrose medium. Interestingly, despite an abundance of essential nutrients transcription levels of functions related to uptake and processing of nitrogen and iron became increased for cultures grown on sucrose as sole carbon source. Transcriptome data were also used to analyze putative regulatory relationships. In addition to the small RNA csrABCD regulon, which seems to play a role in the physiological adaptation and possibly the shift between free-living and plant-associated endophytic life style of Enterobacter sp. 638, our results also pointed to the involvement of rcsAB in controlling responses by Enterobacter sp. 638 to a plant-associated life style. Lastly, targeted mutagenesis was used to confirm this role and showed that compared to wild-type Enterobacter sp. 638 a ΔrcsB mutant was affected in its plant growth promoting ability.« less

  9. Transcriptional Responses to Sucrose Mimic the Plant-Associated Life Style of the Plant Growth Promoting Endophyte Enterobacter sp. 638

    SciTech Connect (OSTI)

    Taghavi, Safiyh; Wu, Xiao; Ouyang, Liming; Zhang, Yian Biao; Stadler, Andrea; McCorkle, Sean; Zhu, Wei; Maslov, Sergei; van der Lelie, Daniel

    2015-01-21

    Growth in sucrose medium was previously found to trigger the expression of functions involved in the plant associated life style of the endophytic bacterium Enterobacter sp. 638. Therefore, comparative transcriptome analysis between cultures grown in sucrose or lactate medium was used to gain insights in the expression levels of bacterial functions involved in the endophytic life style of strain 638. Growth on sucrose as a carbon source resulted in major changes in cell physiology, including a shift from a planktonic life style to the formation of bacterial aggregates. This shift was accompanied by a decrease in transcription of genes involved in motility (e.g. flagella biosynthesis) and an increase in the transcription of genes involved in colonization, adhesion and biofilm formation. The transcription levels of functions previously suggested as being involved in endophytic behavior and functions responsible for plant growth promoting properties, including the synthesis of indole-acetic acid, acetoin and 2,3-butanediol, also increased significantly for cultures grown in sucrose medium. Interestingly, despite an abundance of essential nutrients transcription levels of functions related to uptake and processing of nitrogen and iron became increased for cultures grown on sucrose as sole carbon source. Transcriptome data were also used to analyze putative regulatory relationships. In addition to the small RNA csrABCD regulon, which seems to play a role in the physiological adaptation and possibly the shift between free-living and plant-associated endophytic life style of Enterobacter sp. 638, our results also pointed to the involvement of rcsAB in controlling responses by Enterobacter sp. 638 to a plant-associated life style. Lastly, targeted mutagenesis was used to confirm this role and showed that compared to wild-type Enterobacter sp. 638 a ΔrcsB mutant was affected in its plant growth promoting ability.

  10. Overexpressed KDM5B is associated with the progression of glioma and promotes glioma cell growth via downregulating p21

    SciTech Connect (OSTI)

    Dai, Bin; Hu, Zhiqiang; Huang, Hui; Zhu, Guangtong; Xiao, Zhiyong; Wan, Weiqing; Zhang, Peng; Jia, Wang; Zhang, Liwei

    2014-11-07

    Highlights: • KDM5B is overexpressed in glioma samples. • KDM5B stimulated proliferation of glioma cells. • Inhibition of p21contributes to KDM5B-induced proliferation. - Abstract: Epigenetic alterations such as aberrant expression of histone-modifying enzymes have been implicated in tumorigenesis. Upregulation of lysine (K)-specific demethylase 5B (KDM5B) has been reported in a variety of malignant tumors. However, the impact of KDM5B in glioma remains unclear. The objective of this study was to investigate the expression and prognostic value of KDM5B in glioma. In clinical glioma samples, we found that KDM5B expression was significantly upregulated in cancer lesions compared with normal brain tissues. Kaplan–Meier analysis showed that patients with glioma and higher KDM5B expression tend to have shorter overall survival time. By silencing or overexpressing KDM5B in glioma cells, we found that KDM5B could promote cell growth both in vitro and in vivo. Moreover, we demonstrated that KDM5B promoted glioma proliferation partly via regulation of the expression of p21. Our study provided evidence that KDM5B functions as a novel tumor oncogene in glioma and may be a potential therapeutic target for glioma management.

  11. CDK-associated Cullin 1 promotes cell proliferation with activation of ERK1/2 in human lung cancer A549 cells

    SciTech Connect (OSTI)

    Chen, Tian Jun; Gao, Fei; Yang, Tian; Thakur, Asmitanand; Ren, Hui; Li, Yang; Zhang, Shuo; Wang, Ting; Chen, Ming Wei

    2013-07-19

    Highlights: CDK-associated Cullin 1 (CAC1) expression increases in human lung carcinoma. CAC1 promotes the proliferation of lung cancer A549 cells. CAC1 promotes human lung cancer A549 cell proliferation with activation of ERK1/2. -- Abstract: Lung cancer is one of the most common causes of cancer-related death in the world, but the mechanisms remain unknown. In this study, we investigated the expression of CDK-associated Cullin 1 (CAC1) in lung cancer, the effect of CAC1 on the proliferation of human lung cancer A549 cells, and the activation of signaling pathways of mitogen-activated protein kinases (MAPKs). Results showed that CAC1 expression was higher levels in human lung carcinoma than normal lung tissue, and CAC1 siRNA reduced the proliferation of lung cancer A549 cells by decreasing cell activity and cell division in vitro. The proportion of cells treated with CAC1 siRNA increased in the G1 phase and decreased in the S and G2/M phase, indicative of G1 cell cycle arrest. Furthermore, the proportions of early/late apoptosis in lung cancer A549 cells were enhanced with CAC1 siRNA treatment. It was also found that activation of extracellular signal-regulated protein kinase (ERK) and p38 signaling pathways were involved in the proliferation of A549 cells. After CAC1 siRNA treatment, p-ERK1/2 levels decreased, and meanwhile p-p38 level increased, A549 cell proliferation increased when ERK1/2 signaling is activated by PMA. Our findings demonstrated that CAC1 promoted the proliferation of human lung cancer A549 cells with activation of ERK1/2 signaling pathways, suggesting a potential cure target for treatment of human lung cancer.

  12. Cancer cell-associated cytoplasmic B7–H4 is induced by hypoxia through hypoxia-inducible factor-1α and promotes cancer cell proliferation

    SciTech Connect (OSTI)

    Jeon, You-Kyoung; Park, Sae-Gwang; Choi, Il-Whan; Lee, Soo-Woong; Lee, Sang Min

    2015-04-03

    Aberrant B7–H4 expression in cancer tissues serves as a novel prognostic biomarker for poor survival in patients with cancer. However, the factor(s) that induce cancer cell-associated B7–H4 remain to be fully elucidated. We herein demonstrate that hypoxia upregulates B7–H4 transcription in primary CD138{sup +} multiple myeloma cells and cancer cell lines. In support of this finding, analysis of the Multiple Myeloma Genomics Portal (MMGP) data set revealed a positive correlation between the mRNA expression levels of B7–H4 and the endogenous hypoxia marker carbonic anhydrogenase 9. Hypoxia-induced B7–H4 expression was detected in the cytoplasm, but not in cancer cell membranes. Chromatin immunoprecipitation analysis demonstrated binding of hypoxia-inducible factor-1α (HIF-1α) to proximal hypoxia-response element (HRE) sites within the B7–H4 promoter. Knockdown of HIF-1α and pharmacological inhibition of HIF-1α diminished B7–H4 expression. Furthermore, knockdown of cytoplasmic B7–H4 in MCF-7 decreased the S-phase cell population under hypoxia. Finally, MMGP analysis revealed a positive correlation between the transcript levels of B7–H4 and proliferation-related genes including MKI67, CCNA1, and Myc in several patients with multiple myeloma. Our results provide insight into the mechanisms underlying B7–H4 upregulation and its role in cancer cell proliferation in a hypoxic tumor microenvironment. - Highlights: • Hypoxia upregulates B7–H4 transcription and protein expression. • Hypoxia-induced B7–H4 is detected in the cytoplasm, but not on membrane. • ChIP assay reveals a binding of HIF-1α to B7–H4 promoter at HRE site. • Knockdown and pharmacological inhibition of HIF-1α reduce B7–H4 expression. • B7–H4 knockdown decrease the number of cells in S-phase of cell cycle.

  13. Functional Promoter Variant rs2868371 of HSPB1 Is Associated With Risk of Radiation Pneumonitis After Chemoradiation for Non-Small Cell Lung Cancer

    SciTech Connect (OSTI)

    Pang, Qingsong; Department of Radiation Oncology and Lung Cancer Center, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy, Tianjin ; Wei, Qingyi; Xu, Ting; Yuan, Xianglin; Lopez Guerra, Jose Luis; Levy, Lawrence B.; Liu, Zhensheng; Gomez, Daniel R.; Zhuang, Yan; Wang, Li-E.; Mohan, Radhe; Komaki, Ritsuko; Liao, Zhongxing

    2013-04-01

    Purpose: To date, no biomarkers have been found to predict, before treatment, which patients will develop radiation pneumonitis (RP), a potentially fatal toxicity, after chemoradiation for lung cancer. We investigated potential associations between single nucleotide polymorphisms (SNPs) in HSPB1 and risk of RP after chemoradiation for non-small cell lung cancer (NSCLC). Methods and Materials: Subjects were patients with NSCLC treated with chemoradiation at 1 institution. The training data set comprised 146 patients treated from 1999 to July 2004; the validation data set was 125 patients treated from August 2004 to March 2010. We genotyped 2 functional SNPs of HSPB1 (rs2868370 and rs2868371) from all patients. We used Kaplan-Meier analysis to assess the risk of grade ≥2 or ≥3 RP in both data sets and a parametric log-logistic survival model to evaluate the association of HSPB1 genotypes with that risk. Results: Grade ≥3 RP was experienced by 13% of those with CG/GG and 29% of those with CC genotype of HSPB1 rs2868371 in the training data set (P=.028); corresponding rates in the validation data set were 2% CG/GG and 14% CC (P=.02). Univariate and multivariate analysis confirmed the association of CC of HSPB1 rs2868371 with higher risk of grade ≥3 RP than CG/GG after adjustment for sex, age, performance status, and lung mean dose. This association was validated both in the validation data set and with Harrell's C statistic. Conclusions: The CC genotype of HSPB1 rs2868371 was associated with severe RP after chemoradiation for NSCLC.

  14. Arsenic and chromium in drinking water promote tumorigenesis in a mouse colitis-associated colorectal cancer model and the potential mechanism is ROS-mediated Wnt/β-catenin signaling pathway

    SciTech Connect (OSTI)

    Wang, Xin; Mandal, Ardhendu K.; Saito, Hiroshi; Pulliam, Joseph F.; Lee, Eun Y.; Ke, Zun-Ji; Lu, Jian; Ding, Songze; Li, Li; Shelton, Brent J.; Tucker, Thomas; Evers, B. Mark; Zhang, Zhuo; Shi, Xianglin

    2012-07-01

    Exposure to carcinogenic metals, such as trivalent arsenic [As(III)] and hexavalent chromium [Cr(VI)], through drinking water is a major global public health problem and is associated with various cancers. However, the mechanism of their carcinogenicity remains unclear. In this study, we used azoxymethane/dextran sodium sulfate (AOM/DSS)-induced mouse colitis-associated colorectal cancer model to investigate their tumorigenesis. Our results demonstrate that exposure to As(III) or Cr(VI), alone or in combination, together with AOM/DSS pretreatment has a promotion effect, increasing the colorectal tumor incidence, multiplicity, size, and grade, as well as cell inflammatory response. Two-dimensional differential gel electrophoresis coupled with mass spectrometry revealed that As(III) or Cr(VI) treatment alone significantly changed the density of proteins. The expression of β-catenin and phospho-GSK was increased by treatment of carcinogenic metals alone. Concomitantly, the expression of NADPH oxidase1 (NOX1) and the level of 8-OHdG were also increased by treatment of carcinogenic metals alone. Antioxidant enzymes, such as superoxide dismutase (SOD) and catalase, were decreased. Similarly, in an in vitro system, exposure of CRL-1807 to carcinogenic metals increased reactive oxygen species (ROS) generation, the expression of β-catenin, phospho-GSK, and NOX1. Inhibition of ROS generation by addition of SOD or catalase inhibited β-catenin expression and activity. Our study provides a new animal model to study the carcinogenicity of As(III) and Cr(VI) and suggests that As(III) and Cr(VI) promote colorectal cancer tumorigenesis, at least partly, through ROS-mediated Wnt/β-catenin signaling pathway. -- Highlights: ► Carcinogenic metals in drinking water promote colorectal tumor formation in vivo. ► Carcinogenic metals induce β-catenin activation in vivo and in vitro. ► ROS generation induced by carcinogenic metals mediated β-catenin activation.

  15. Commercial Current Promotions

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New Commercial Program Development Commercial Current Promotions Industrial Federal Agriculture This page features all current special promotions for commercial programs....

  16. High expression Zymomonas promoters

    DOE Patents [OSTI]

    Viitanen, Paul V.; Tao, Luan; Zhang, Yuying; Caimi, Perry G.; McCole, Laura : Zhang, Min; Chou, Yat-Chen; McCutchen, Carol M.; Franden, Mary Ann

    2011-08-02

    Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.

  17. Environmental Markets Association | Open Energy Information

    Open Energy Info (EERE)

    20005 Product: The Environmental Markets Association (EMA), an international nonprofit trade association, was originally formed in 1997 to promote market-based trading solutions...

  18. Antonya Sanders-Promoting

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Antonya Sanders-Promoting nanoscience integration through outreach March 17, 2014 Promoting nanoscience integration through outreach Although raised in Los Alamos, Antonya Sanders knows what it is like to be a foreigner. The daughter of a Czech immigrant, Sanders lived in England and traveled the world to find ways to help others, including aiding Bosnian refugees. She lived on the Pacific island of Kiribati, where she was a Peace Corps' volunteer and teacher for three years, living in a thatch

  19. Genome-wide analysis of promoter architecture in Drosophila melanogaster

    SciTech Connect (OSTI)

    Hoskins, Roger A.; Landolin, Jane M.; Brown, James B.; Sandler, Jeremy E.; Takahashi, Hazuki; Lassmann, Timo; Yu, Charles; Booth, Benjamin W.; Zhang, Dayu; Wan, Kenneth H.; Yang, Li; Boley, Nathan; Andrews, Justen; Kaufman, Thomas C.; Graveley, Brenton R.; Bickel, Peter J.; Carninci, Piero; Carlson, Joseph W.; Celniker, Susan E.

    2010-10-20

    Core promoters are critical regions for gene regulation in higher eukaryotes. However, the boundaries of promoter regions, the relative rates of initiation at the transcription start sites (TSSs) distributed within them, and the functional significance of promoter architecture remain poorly understood. We produced a high-resolution map of promoters active in the Drosophila melanogaster embryo by integrating data from three independent and complementary methods: 21 million cap analysis of gene expression (CAGE) tags, 1.2 million RNA ligase mediated rapid amplification of cDNA ends (RLMRACE) reads, and 50,000 cap-trapped expressed sequence tags (ESTs). We defined 12,454 promoters of 8037 genes. Our analysis indicates that, due to non-promoter-associated RNA background signal, previous studies have likely overestimated the number of promoter-associated CAGE clusters by fivefold. We show that TSS distributions form a complex continuum of shapes, and that promoters active in the embryo and adult have highly similar shapes in 95% of cases. This suggests that these distributions are generally determined by static elements such as local DNA sequence and are not modulated by dynamic signals such as histone modifications. Transcription factor binding motifs are differentially enriched as a function of promoter shape, and peaked promoter shape is correlated with both temporal and spatial regulation of gene expression. Our results contribute to the emerging view that core promoters are functionally diverse and control patterning of gene expression in Drosophila and mammals.

  20. German Wind Energy Association | Open Energy Information

    Open Energy Info (EERE)

    German Wind Energy Association Place: Osnabrck, Germany Zip: 49074 Sector: Wind energy Product: Assocation for the promotion of wind energy in Germany. References: German Wind...

  1. US Integrated Waste Services Association | Open Energy Information

    Open Energy Info (EERE)

    Integrated Waste Services Association Jump to: navigation, search Name: US Integrated Waste Services Association Place: Washington, DC Product: Focussed on promoting integrated...

  2. Crystallographic Association Fellow

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    named American Crystallographic Association Fellow January 4, 2013 The American Crystallographic Association (ACA) honored Thomas Terwilliger of LANL's Biosecurity and Public Health group with the rank of Fellow. He was inducted during the ACA annual meeting in Boston. The ACA Fellows program celebrates the excellence of its members and promotes their recognition worldwide. ACA Fellows serve as scientific ambassadors to the broader scientific community and the general public to advance science

  3. Promoters and proteins from Clostridium thermocellum and uses thereof

    DOE Patents [OSTI]

    Wu, J. H. David; Newcomb, Michael

    2012-11-13

    The present invention relates to an inducible and a high expression nucleic acid promoter isolated from Clostridium thermocellum. These promoters are useful for directing expression of a protein or polypeptide encoded by a nucleic acid molecule operably associated with the nucleic acid promoters. The present invention also relates to nucleic acid constructs including the C. thermocellum promoters, and expression vectors and hosts containing such nucleic acid constructs. The present invention also relates to protein isolated from Clostridium thermocellum, including a repressor protein. The present invention also provides methods of using the isolated promoters and proteins from Clostridium thermocellum, including methods for directing inducible in vitro and in vivo expression of a protein or polypeptide in a host, and methods of producing ethanol from a cellulosic biomass.

  4. Secretary Bodman Promotes Energy Bill to Western Governors | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Promotes Energy Bill to Western Governors Secretary Bodman Promotes Energy Bill to Western Governors March 1, 2005 - 10:37am Addthis WASHINGTON, DC - U.S. Secretary of Energy Samuel W. Bodman in a speech before the Western Governors Association today expressed the need for Congress to pass comprehensive energy legislation and highlighted the benefits of the proposal for the western United States. Secretary Bodman also discussed a number of important energy initiatives including:

  5. ORISE: Iddins promoted to associate director of Radiation Emergency...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Prior to joining ORAU, Iddins served as a staff physician for the Methodist Medical Center Healthworks and Roane Industrial Medicine where she performed occupational health ...

  6. Photovoltaic module with adhesion promoter

    DOE Patents [OSTI]

    Xavier, Grace

    2013-10-08

    Photovoltaic modules with adhesion promoters and methods for fabricating photovoltaic modules with adhesion promoters are described. A photovoltaic module includes a solar cell including a first surface and a second surface, the second surface including a plurality of interspaced back-side contacts. A first glass layer is coupled to the first surface by a first encapsulating layer. A second glass layer is coupled to the second surface by a second encapsulating layer. At least a portion of the second encapsulating layer is bonded directly to the plurality of interspaced back-side contacts by an adhesion promoter.

  7. Cu(II) promotes amyloid pore formation

    SciTech Connect (OSTI)

    Zhang, Hangyu; Rochet, Jean-Christophe; Stanciu, Lia A.

    2015-08-14

    The aggregation of α-synuclein is associated with dopamine neuron death in Parkinson's disease. There is controversy in the field over the question of which species of the aggregates, fibrils or protofibrils, are toxic. Moreover, compelling evidence suggested the exposure to heavy metals to be a risk of PD. Nevertheless, the mechanism of metal ions in promoting PD remains unclear. In this research, we investigated the structural basis of Cu(II) induced aggregation of α-synuclein. Using transmission electron microscopy experiments, Cu(II) was found to promote in vitro aggregation of α-synuclein by facilitating annular protofibril formation rather than fibril formation. Furthermore, neuroprotective baicalein disaggregated annular protofibrils accompanied by considerable decrease of β-sheet content. These results strongly support the hypothesis that annular protofibrils are the toxic species, rather than fibrils, thereby inspiring us to search novel therapeutic strategies for the suppression of the toxic annular protofibril formation. - Highlights: • Cu(II) promoted the annular protofibril formation of α-synuclein in vitro. • Cu(II) postponed the in vitro fibrillization of α-synuclein. • Neuroprotective baicalein disaggregated annular protofibrils.

  8. Consumer Electronics Association Comment | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Comment Consumer Electronics Association Comment The Consumer Electronics Association (CEA) is the preeminent trade association promoting growth in the $285 billion U.S. consumer electronics industry. CEA comments re DOE Regulatory Burden RFI_7-17-15 (164.06 KB) More Documents & Publications Consumer Electronics Association Comments

  9. Blog Promotion | Open Energy Information

    Open Energy Info (EERE)

    and are interested in blogging, you may want to promote your blog using OpenEI's social media and rss feed channels. Here is a simple list of information on how to have...

  10. Initiation Complex Structure and Promoter Proofreading (Journal...

    Office of Scientific and Technical Information (OSTI)

    Initiation Complex Structure and Promoter Proofreading Citation Details In-Document Search Title: Initiation Complex Structure and Promoter Proofreading Authors: Liu, Xin ; ...

  11. Renewable Fuels Assocation | Open Energy Information

    Open Energy Info (EERE)

    DC Zip: 20001 Sector: Renewable Energy Product: US national trade association for the ethanol industry, the Renewable Fuels Association (RFA) has been working as the "Voice of the...

  12. Tamil Nadu Spinning Mills Association TASMA | Open Energy Information

    Open Energy Info (EERE)

    Tamil Nadu, India Zip: 624003 Sector: Wind energy Product: Association of spinning mill owners; promoting wind power projects. References: Tamil Nadu Spinning Mills...

  13. Students' Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Resources Living in Los Alamos Ombuds Program Scholarships Student Association Autobiographies Student Programs Advisory Committee (internal) 2015 Student Liaison Contact List

  14. Students' Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Living in Los Alamos Ombuds Program Scholarships Student Association Autobiographies Student Programs Advisory Committee (internal) 2015 Student Liaison Contact List (pdf)

  15. RFA-14-0002 - In the Matter of Highway Oil, Inc. | Department of Energy

    Energy Savers [EERE]

    REVISED MD-110 REFERENCE GUIDE SEPTEMBER 2015 BACKGROUND On August 5, 2015, the Equal Employment Opportunity Commission (EEOC) approved the first revision to its Management Directive 110 (MD-110) since 1999. The revised MD-110 provides federal agencies with updated Commission policies, procedures, and guidance relating to the federal sector complaint process as set forth in 29 C.F.R. Part 1614 and reflects new developments in case law, as the federal workplace and EEO practices have evolved. The

  16. Utility Promoters for Biomass Feedstock Biotechnology - Energy...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Utility Promoters for Biomass Feedstock Biotechnology Inventors: Kyung-Hwan Han, Jae-Heung ... Successful application of biotechnology requires both gene discovery and a proper means ...

  17. How Does Your Community Promote Energy Savings?

    Broader source: Energy.gov [DOE]

    John works with various organizations in his community to promote energy savings, and he has found that none of these organizations can do it alone.

  18. Consumer Electronics Association Comments | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Comments Consumer Electronics Association Comments The Consumer Electronics Association (CEA) is the preeminent trade association promoting growth in the $285 billion U.S. consumer electronics industry. CEA represents more than 2,000 corporate members involved in the design, development, manufacturing, distribution and integration of audio, video, in-vehicle electronics, wireless and landline communications, information technology, home networking, multimedia and accessory products, as well as

  19. Statistical Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Kelly named Fellow of the American Statistical Association August 2, 2016 The American Statistical Association (ASA) has honored Elizabeth Kelly of the Lab's Statistical Sciences group with the title of Fellow. The ASA recognized her for providing exemplary statistical leadership of and collaboration on multidisciplinary teams dealing with environmental restoration, weapon quality, and nuclear materials storage to ensure the safety and security of the Nation. She will receive the Fellow award at

  20. Obama Officials Announce Steps to Promote the Clean Energy Potential of the

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    West | Department of Energy Obama Officials Announce Steps to Promote the Clean Energy Potential of the West Obama Officials Announce Steps to Promote the Clean Energy Potential of the West June 15, 2009 - 4:03pm Addthis Secretaries Chu, Salazar and Vilsack and Chairs Sutley and Wellinghoff announce energy and transmission policies at the Western Governors' Association Annual Meeting PARK CITY, UTAH - Senior Obama Administration officials today announced a number of steps that will help the

  1. DOE Launches EnergySmart Hospitals to Promote Improved Energy Efficiency in

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Healthcare | Department of Energy EnergySmart Hospitals to Promote Improved Energy Efficiency in Healthcare DOE Launches EnergySmart Hospitals to Promote Improved Energy Efficiency in Healthcare July 23, 2008 - 2:14pm Addthis Initiative Targets Energy and Cost Savings in Hospitals Across the United States WASHINGTON - U.S. Department of Energy (DOE) Associate Under Secretary for Energy Richard F. Moorer today announced the launch of the EnergySmart Hospitals initiative to increase the use of

  2. Workplace Charging Challenge: Promote Charging at Work

    Broader source: Energy.gov [DOE]

    Employees with access to workplace charging are six times more likely to drive a plug-in electric vehicle (PEV) than the average worker. Promoting PEV charging at workplaces is one great way that...

  3. Extracellular Proteins Promote Zinc Sulfide Aggregation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Extracellular Proteins Promote Zinc Sulfide Aggregation Extracellular Proteins Promote Zinc Sulfide Aggregation Print Wednesday, 26 September 2007 00:00 Researchers from the ALS, Berkeley Lab's National Center for Electron Microscopy (NCEM), and Lawrence Livermore National Laboratory analyzed biofilm samples rich in zinc sulfide and dominated by sulfate-reducing bacteria, which were collected from lead-zinc mine waters. The researchers were curious about the relationship of the organic material

  4. Alternative Fuels Data Center: San Diego Leads in Promoting EVs

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    San Diego Leads in Promoting EVs to someone by E-mail Share Alternative Fuels Data Center: San Diego Leads in Promoting EVs on Facebook Tweet about Alternative Fuels Data Center: San Diego Leads in Promoting EVs on Twitter Bookmark Alternative Fuels Data Center: San Diego Leads in Promoting EVs on Google Bookmark Alternative Fuels Data Center: San Diego Leads in Promoting EVs on Delicious Rank Alternative Fuels Data Center: San Diego Leads in Promoting EVs on Digg Find More places to share

  5. Vega Biofuels Inc formerly Vega Promotional Systems | Open Energy...

    Open Energy Info (EERE)

    Vega Biofuels Inc formerly Vega Promotional Systems Jump to: navigation, search Name: Vega Biofuels Inc (formerly Vega Promotional Systems) Place: Norcross, Georgia Zip: 30010...

  6. Energy 101: Promoting Energy Education in the Nation's Colleges...

    Office of Environmental Management (EM)

    Energy 101: Promoting Energy Education in the Nation's Colleges and Universities Energy 101: Promoting Energy Education in the Nation's Colleges and Universities June 25, 2014 - ...

  7. Policies for Promoting Industrial Energy Efficiency in Developing...

    Open Energy Info (EERE)

    Promoting Industrial Energy Efficiency in Developing Countries and Transition Economies Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Policies for Promoting Industrial...

  8. Department of Energy Announces $15 Million to Promote Innovative...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    15 Million to Promote Innovative Geothermal Heat Recovery Methods and Technologies Department of Energy Announces 15 Million to Promote Innovative Geothermal Heat Recovery...

  9. United States, Australia, and Iceland to Promote Geothermal Energy...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    United States, Australia, and Iceland to Promote Geothermal Energy United States, Australia, and Iceland to Promote Geothermal Energy August 28, 2008 - 12:43pm Addthis Photo of ...

  10. Environmental Law Institute Webinar to Promote Superior Energy...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Environmental Law Institute Webinar to Promote Superior Energy Performance(tm) on July 22, 2014 Environmental Law Institute Webinar to Promote Superior Energy Performance(tm) on ...

  11. Energy and EPA Webinar Series Promotes Tribal Energy Sufficiency...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Promotes Tribal Energy Sufficiency and Economic Development Energy and EPA Webinar Series Promotes Tribal Energy Sufficiency and Economic Development January 20, 2012 - 11:40am ...

  12. Energy Portfolio Standards and the Promotion of Combined Heat...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Portfolio Standards and the Promotion of Combined Heat and Power (CHP) White Paper, April 2009 Energy Portfolio Standards and the Promotion of Combined Heat and Power (CHP) White ...

  13. Optimized scalar promotion with load and splat SIMD instructions...

    Office of Scientific and Technical Information (OSTI)

    Optimized scalar promotion with load and splat SIMD instructions Citation Details In-Document Search Title: Optimized scalar promotion with load and splat SIMD instructions ...

  14. National Programme for the Promotion of Solar Energy | Open Energy...

    Open Energy Info (EERE)

    Programme for the Promotion of Solar Energy Jump to: navigation, search Name National Programme for the Promotion of Solar Energy AgencyCompany Organization Deutsche Gesellschaft...

  15. Centre for Jatropha Promotion Biodiesel | Open Energy Information

    Open Energy Info (EERE)

    Jatropha Promotion Biodiesel Jump to: navigation, search Name: Centre for Jatropha Promotion & Biodiesel Place: Churu, Rajasthan, India Zip: 331001 Product: Indian-based non-profit...

  16. UNIDO Investment and Technology Promotion | Open Energy Information

    Open Energy Info (EERE)

    Investment and Technology Promotion Jump to: navigation, search Name UNIDO Investment and Technology Promotion AgencyCompany Organization United Nations Industrial Development...

  17. Promoting a Green Economy through Clean Transportation Alternatives...

    Broader source: Energy.gov (indexed) [DOE]

    KB) More Documents & Publications Promoting a Green Economy through Clean Transportation Alternatives Promoting a Green Economy through Clean Transportation Alternatives EV ...

  18. Promoting a Green Economy through Clean Transportation Alternatives...

    Broader source: Energy.gov (indexed) [DOE]

    Promoting a Green Economy through Clean Transportation Alternatives Promoting a Green Economy through Clean Transportation Alternatives Town of Hempstead: Project Energy, From ...

  19. Shuchi Energy Ad Promotions P Ltd | Open Energy Information

    Open Energy Info (EERE)

    Shuchi Energy Ad Promotions P Ltd Jump to: navigation, search Name: Shuchi Energy Ad Promotions (P) Ltd Place: Hyderabad, Andhra Pradesh, India Zip: 500062 Sector: Solar Product:...

  20. PRESS RELEASE: Community Leaders Institutes (CLIs) Promote Awareness...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PRESS RELEASE: Community Leaders Institutes (CLIs) Promote Awareness of Climate Change Impacts PRESS RELEASE: Community Leaders Institutes (CLIs) Promote Awareness of Climate Change ...

  1. Universal light-switchable gene promoter system

    DOE Patents [OSTI]

    Quail, Peter H.; Huq, Enamul; Tepperman, James; Sato, Sae

    2005-02-22

    An artificial promoter system that can be fused upstream of any desired gene enabling reversible induction or repression of the expression of the gene at will in any suitable host cell or organisms by light is described. The design of the system is such that a molecule of the plant photoreceptor phytochrome is targeted to the specific DNA binding site in the promoter by a protein domain that is fused to the phytochrome and that specifically recognizes this binding site. This bound phytochrome, upon activation by light, recruits a second fusion protein consisting of a protein that binds to phytochrome only upon light activation and a transcriptional activation domain that activates expression of the gene downstream of the promoter.

  2. Promotion of Efficient Use of Energy

    SciTech Connect (OSTI)

    Harry Misuriello; DOE Project Officer - Keith Bennett

    2006-01-25

    The Department of Energy funded the Alliance to Save Energy to promote the efficient use of energy under a multiyear cooperative agreement. This funding allowed the Alliance to be innovative and flexible in its program development, and to initiate and enhance projects it would otherwise not have been able to pursue. The program period was 1999 through 2004. The mission of the Alliance to Save Energy is to promote energy efficiency domestically and worldwide. The Alliance followed this mission by working closely with consumers, government, policy makers, and energy efficient product and service providers. The projects that were initiated by the Alliance included communication and consumer education, policy analysis and research, the promotion of interaction among the energy efficiency industry, and international energy efficiency programs. The funding from the Department of Energy allowed the Alliance to study new issues in energy efficiency, draw public attention to those issues, and create targeted programs, such as the Efficient Windows Collaborative or the Green Schools program, which now function on their own to promote energy efficiency in important areas.

  3. Method for promoting Michael addition reactions

    DOE Patents [OSTI]

    Shah, Pankaj V.; Vietti, David E.; Whitman, David William

    2010-09-21

    Homogeneously dispersed solid reaction promoters having an average particle size from 0.01 .mu.m to 500 .mu.m are disclosed for preparing curable mixtures of at least one Michael donor and at least one Michael acceptor. The resulting curable mixtures are useful as coatings, adhesives, sealants and elastomers.

  4. Promoting energy efficiency in reforming electricity markets

    SciTech Connect (OSTI)

    Clinton, J.; Kozloff, K.

    1998-07-01

    Many developing countries are initiating power sector reforms to stimulate private investment, increase operation and management efficiencies, and recover the full costs of power. Reforms may include unbundling generation, transmission, distribution and retail services; commercial management; competition; and private ownership. This paper draws upon six country case studies--Argentina, Chile, New Zealand, Norway, the United Kingdom, and the US--to identify major models of power reforms and their implications for energy efficiency--both positive and negative. There are both structural and institutional features of reform that may discourage commercial offerings of end-use efficiency services. Valuable lessons are discussed regarding what reforms and policies have worked to promote energy efficiency and which have not. Several models are offered for how developing countries can promote energy efficiency under some of the more common forms of power sector restructuring. Conclusions and recommendations are directed at key decision-makers in developing countries contemplating power sector reforms.

  5. Extracellular Proteins Promote Zinc Sulfide Aggregation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Extracellular Proteins Promote Zinc Sulfide Aggregation Print Researchers from the ALS, Berkeley Lab's National Center for Electron Microscopy (NCEM), and Lawrence Livermore National Laboratory analyzed biofilm samples rich in zinc sulfide and dominated by sulfate-reducing bacteria, which were collected from lead-zinc mine waters. The researchers were curious about the relationship of the organic material and metals, particularly how organics affect mobility, and its potential for

  6. Extracellular Proteins Promote Zinc Sulfide Aggregation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Extracellular Proteins Promote Zinc Sulfide Aggregation Print Researchers from the ALS, Berkeley Lab's National Center for Electron Microscopy (NCEM), and Lawrence Livermore National Laboratory analyzed biofilm samples rich in zinc sulfide and dominated by sulfate-reducing bacteria, which were collected from lead-zinc mine waters. The researchers were curious about the relationship of the organic material and metals, particularly how organics affect mobility, and its potential for

  7. Extracellular Proteins Promote Zinc Sulfide Aggregation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Extracellular Proteins Promote Zinc Sulfide Aggregation Print Researchers from the ALS, Berkeley Lab's National Center for Electron Microscopy (NCEM), and Lawrence Livermore National Laboratory analyzed biofilm samples rich in zinc sulfide and dominated by sulfate-reducing bacteria, which were collected from lead-zinc mine waters. The researchers were curious about the relationship of the organic material and metals, particularly how organics affect mobility, and its potential for

  8. Extracellular Proteins Promote Zinc Sulfide Aggregation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Extracellular Proteins Promote Zinc Sulfide Aggregation Print Researchers from the ALS, Berkeley Lab's National Center for Electron Microscopy (NCEM), and Lawrence Livermore National Laboratory analyzed biofilm samples rich in zinc sulfide and dominated by sulfate-reducing bacteria, which were collected from lead-zinc mine waters. The researchers were curious about the relationship of the organic material and metals, particularly how organics affect mobility, and its potential for

  9. Extracellular Proteins Promote Zinc Sulfide Aggregation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Extracellular Proteins Promote Zinc Sulfide Aggregation Print Researchers from the ALS, Berkeley Lab's National Center for Electron Microscopy (NCEM), and Lawrence Livermore National Laboratory analyzed biofilm samples rich in zinc sulfide and dominated by sulfate-reducing bacteria, which were collected from lead-zinc mine waters. The researchers were curious about the relationship of the organic material and metals, particularly how organics affect mobility, and its potential for

  10. Extracellular Proteins Promote Zinc Sulfide Aggregation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Extracellular Proteins Promote Zinc Sulfide Aggregation Print Researchers from the ALS, Berkeley Lab's National Center for Electron Microscopy (NCEM), and Lawrence Livermore National Laboratory analyzed biofilm samples rich in zinc sulfide and dominated by sulfate-reducing bacteria, which were collected from lead-zinc mine waters. The researchers were curious about the relationship of the organic material and metals, particularly how organics affect mobility, and its potential for

  11. Promoting Sustainability on a Global Scale

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Promoting Sustainability on a Global Scale Biomass 2013 Washington, 1 August 2013 Martina Otto Head of Policy Unit, Energy Branch United Nations Environment Programme www.unep.org/energy/bioenergy UNEP Global Environment Outlook - GEO-5: We are already observing changes to the Earth System, unprecedented in human history. Efforts to slow the rate or extent of change have resulted in moderate successes but have not succeeded in reversing adverse environmental changes. We need transformational

  12. Obama Officials Announce Steps to Promote the Clean Energy Potential...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Obama Officials Announce Steps to Promote the Clean Energy Potential of the West Obama Officials Announce Steps to Promote the Clean Energy Potential of the West June 15, 2009 -...

  13. Active hopper for promoting flow of bulk granular or powdered...

    Office of Scientific and Technical Information (OSTI)

    Data Explorer Search Results Active hopper for promoting flow of bulk granular or powdered ... An apparatus that promotes the flow of materials has a body having an inner shape for ...

  14. DOE Promotion of ISO 50001 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ISO 50001 Energy Management Standard » DOE Promotion of ISO 50001 DOE Promotion of ISO 50001 The U.S. Department of Energy (DOE) played a critical role in developing ISO 50001-the world's first global energy management system standard. ISO 50001 promotes the widespread adoption of best practices and drives investment in energy efficiency. DOE actively supports its broad implementation to achieve powerful benefits to U.S. businesses and to the nation. Learn how various DOE offices are promoting

  15. Workplace Charging Challenge: Promote Charging at Work | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Promote Charging at Work Workplace Charging Challenge: Promote Charging at Work Workplace Charging Challenge: Promote Charging at Work Employees with access to workplace charging are six times more likely to drive a plug-in electric vehicle (PEV) than the average worker. Promoting PEV charging at workplaces is one great way that states, cities and other organizations can encourage PEV adoption in their communities. Use the material below to engage and educate employers about the

  16. Method for using a yeast alpha-amylase promoter

    DOE Patents [OSTI]

    Gao, Johnway; Skeen, Rodney S.; Hooker, Brian S.; Anderson, Daniel B.

    2003-04-22

    The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

  17. RFA-14-0001- In the Matter of Commonwealth Oil Refining Company, Inc./Commonwealth of Puerto Rico

    Broader source: Energy.gov [DOE]

    On July 29, 2014, OHA approved a distribution plan submitted by the Commonwealth of Puerto Rico (Puerto Rico) to use approximately $1.2 million of petroleum overcharge funds held in escrow by the...

  18. Diesel Engine CO2 and SOx Emission Compliance Strategy for the Royal Navy (RN) and Royal Fleet Auxiliary (RFA) Flotillas

    Broader source: Energy.gov [DOE]

    Poster presentation from the 2007 Diesel Engine-Efficiency & Emissions Research Conference (DEER 2007). 13-16 August, 2007, Detroit, Michigan. Sponsored by the U.S. Department of Energy's (DOE) Office of FreedomCAR and Vehicle Technologies (OFCVT).

  19. Postdocs - Berkeley Lab Postdoc Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Postdocs - Berkeley Lab Postdoc Association

  20. Brookhaven Science Associates, LLC

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Doon L. Gibbs Laboratory Director Brookhaven Science Associates, LLC Brookhaven National ... on November 12, 2014. Brookhaven Science Associates, LLC (BSA) documented this ...

  1. MRG15 activates the cdc2 promoter via histone acetylation in human cells

    SciTech Connect (OSTI)

    Pena, AndreAna N.; Tominaga, Kaoru; Pereira-Smith, Olivia M.; Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, TX

    2011-07-01

    Chromatin remodeling is required for transcriptional activation and repression. MRG15 (MORF4L1), a chromatin modulator, is a highly conserved protein and is present in complexes containing histone acetyltransferases (HATs) as well as histone deacetylases (HDACs). Loss of expression of MRG15 in mice and Drosophila results in embryonic lethality and fibroblast and neural stem/progenitor cells cultured from Mrg15 null mouse embryos exhibit marked proliferative defects when compared with wild type cells. To determine the role of MRG15 in cell cycle progression we performed chromatin immunoprecipitation with an antibody to MRG15 on normal human fibroblasts as they entered the cell cycle from a quiescent state, and analyzed various cell cycle gene promoters. The results demonstrated a 3-fold increase in MRG15 occupancy at the cdc2 promoter during S phase of the cell cycle and a concomitant increase in acetylated histone H4. H4 lysine 12 was acetylated at 24 h post-serum stimulation while there was no change in acetylation of lysine 16. HDAC1 and 2 were decreased at this promoter during cell cycle progression. Over-expression of MRG15 in HeLa cells activated a cdc2 promoter-reporter construct in a dose-dependent manner, whereas knockdown of MRG15 resulted in decreased promoter activity. In order to implicate HAT activity, we treated cells with the HAT inhibitor anacardic acid and determined that HAT inhibition results in loss of expression of cdc2 mRNA. Further, chromatin immunoprecipitation with Tip60 localizes the protein to the same 110 bp stretch of the cdc2 promoter pulled down by MRG15. Additionally, we determined that cotransfection of MRG15 with the known associated HAT Tip60 had a cooperative effect in activating the cdc2 promoter. These results suggest that MRG15 is acting in a HAT complex involving Tip60 to modify chromatin via acetylation of histone H4 at the cdc2 promoter to activate transcription.

  2. Promoting Sustainability on a Global Scale | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Promoting Sustainability on a Global Scale Promoting Sustainability on a Global Scale Promoting Sustainability on a Global Scale Martina Otto, Head of the Policy Unit, United Nations Environment Programme b13_otto_sustianability.pdf (715.08 KB) More Documents & Publications Sustainability for the Global Biofuels Industry Minimizing Risks and Maximizing Opportunities Sustainability for the Global Biofuels Industry: Minimizing Risks and Maximizing Opportunities Bioenergy Technologies Office

  3. State and Regional policies that promote energy efficiency programs carried

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    out by electric and gas utilities | Department of Energy and Regional policies that promote energy efficiency programs carried out by electric and gas utilities State and Regional policies that promote energy efficiency programs carried out by electric and gas utilities A report to the United States Congress Pursuant to section 139 of the energy policy act of 2005. March 2007 State and Regional policies that promote energy efficiency programs carried out by electric and gas utilities (1.42

  4. Cybersecurity Awareness Marketing/Promotional Material | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Marketing/Promotional Material Cybersecurity Awareness Marketing/Promotional Material The OCIO has developed a variety of cyber awareness marketing and promotional materials available for download to DOE organizations, and public and private institutions. 2013 NCSAM Announcement (94.49 KB) Supply Chain Risk Management Awareness (50.09 KB) A Case of Mistaken Identity Communication (148.91 KB) 2012 NCSAM Postcards (152.1 KB) A Case of Mistaken Identity Word Search (182.8 KB) 2012 NCSAM

  5. State and regional policies that promote energy efficiency programs carried

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    out by electric and gas utilities | Department of Energy and regional policies that promote energy efficiency programs carried out by electric and gas utilities State and regional policies that promote energy efficiency programs carried out by electric and gas utilities A report to the United States Congress Pursuant to section 139 of the Energy Policy Act of 2005. March 2007 State and regional policies that promote energy efficiency programs carried out by electric and gas utilities (1.42

  6. Secretary Chu Announces New Efforts to Promote Clean Energy in...

    Broader source: Energy.gov (indexed) [DOE]

    Up to 10 million will be available this year through DOE's Tribal Energy Program to support ... renewable energy resources, and promote economic development for tribal communities. ...

  7. Top 15 Ways to Promote PEVs at Work

    Broader source: Energy.gov [DOE]

    Your decision to offer workplace charging can increase PEV awareness among current and potential employees. Consider organizing the activities below to promote workplace charging.

  8. Optimized scalar promotion with load and splat SIMD instructions...

    Office of Scientific and Technical Information (OSTI)

    Search Title: Optimized scalar promotion with load and splat SIMD instructions Mechanisms for optimizing scalar code executed on a single instruction multiple data (SIMD) engine ...

  9. Inhibition of Sirt1 promotes neural progenitors toward motoneuron...

    Office of Scientific and Technical Information (OSTI)

    Sirt1, to promote neural precursor cell (NPC) development during differentiation ... in the development of hESC-based cell therapy in motoneuron disease. less ...

  10. South Africa-Promoting Low Emission Urban Development Strategies...

    Open Energy Info (EERE)

    search Name South Africa-Promoting Low Emission Urban Development Strategies in Emerging Economy Countries (URBAN-LEDS) AgencyCompany Organization ICLEI - Local Governments for...

  11. Publicly Backed Guarantees as Policy Instruments to Promote Clean...

    Open Energy Info (EERE)

    Publicly Backed Guarantees as Policy Instruments to Promote Clean Energy Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Publicly Backed Guarantees as Policy Instruments...

  12. Senegal-GTZ Programme to Promote Rural Electrification and a...

    Open Energy Info (EERE)

    in producing and marketing their products, and also promotes companies which produce alternative fuels from waste materials and reeds. This lightens the workload of women, who...

  13. Investigations of element spatial correlation in Mn-promoted...

    Office of Scientific and Technical Information (OSTI)

    Investigations of element spatial correlation in Mn-promoted Co-based Fischer-Tropsch synthesis catalysts This content will become publicly available on June 4, 2017 Title: ...

  14. State and regional policies that promote energy efficiency programs...

    Broader source: Energy.gov (indexed) [DOE]

    State and Regional policies that promote energy efficiency programs carried out by electric and gas utilities Chapter 3: Demand-Side Resources Benefits of Demand Response in ...

  15. State and Regional policies that promote energy efficiency programs...

    Office of Environmental Management (EM)

    State and regional policies that promote energy efficiency programs carried out by electric and gas utilities Chapter 3: Demand-Side Resources Benefits of Demand Response in ...

  16. Solar Promotion International GmbH | Open Energy Information

    Open Energy Info (EERE)

    International GmbH Place: Pforzheim, Germany Zip: 75172 Sector: Solar Product: German organiser of global solar conferences. References: Solar Promotion International...

  17. Ghana-GTZ Electrification Component of the Promotion of Private...

    Open Energy Info (EERE)

    Topics Background analysis Website http:www.gtz.deenthemenum Country Ghana Western Africa References Electrification Component of the Promotion of Private Sector Programme...

  18. A Model for the Nation: Promoting Education and Innovation in...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Model for the Nation: Promoting Education and Innovation in Vermont's Electricity Sector - ... Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy ...

  19. Building Bridges: Federal Agencies Join Forces to Promote Sustainable...

    Broader source: Energy.gov (indexed) [DOE]

    tribal communities by: 1. Promoting sustainable economic development; 2. Supporting ... and 5. Protecting and supporting the sustainable management of Native lands, ...

  20. Nepali Alternative Energy Promotion Centre (AEPC) | Open Energy...

    Open Energy Info (EERE)

    is a Government institution established on November 3, 1996 under the then Ministry of Science and Technology with the objective of developing and promoting renewablealternative...

  1. The oncoprotein HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote the proliferation of breast cancer cells

    SciTech Connect (OSTI)

    Zhang, Yingyi; Zhao, Yu; Li, Leilei; Shen, Yu; Cai, Xiaoli; Zhang, Xiaodong; Ye, Lihong

    2013-05-03

    Highlights: HBXIP is able to upregulate the expression of PDGFB in breast cancer cells. HBXIP serves as a coactivator of activating transcription factor Sp1. HBXIP stimulates the PDGFB promoter via activating transcription factor Sp1. HBXIP promotes the proliferation of breast cancer cell via upregulating PDGFB. -- Abstract: We have reported that the oncoprotein hepatitis B virus X-interacting protein (HBXIP) acts as a novel transcriptional coactivator to promote proliferation and migration of breast cancer cells. Previously, we showed that HBXIP was able to activate nuclear factor-?B (NF-?B) in breast cancer cells. As an oncogene, the platelet-derived growth factor beta polypeptide (PDGFB) plays crucial roles in carcinogenesis. In the present study, we found that both HBXIP and PDGFB were highly expressed in breast cancer cell lines. Interestingly, HBXIP was able to increase transcriptional activity of NF-?B through PDGFB, suggesting that HBXIP is associated with PDGFB in the cells. Moreover, HBXIP was able to upregulate PDGFB at the levels of mRNA, protein and promoter in the cells. Then, we identified that HBXIP stimulated the promoter of PDGFB through activating transcription factor Sp1. In function, HBXIP enhanced the proliferation of breast cancer cells through PDGFB in vitro. Thus, we conclude that HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote proliferation of breast cancer cells.

  2. Regulatory Promotion of Emergent CCS Technology

    SciTech Connect (OSTI)

    Davies, Lincoln; Uchitel, Kirsten; Johnson, David

    2014-01-01

    Despite the growing inevitability of climate change and the attendant need for mitigation strategies, carbon capture and sequestration (CCS) has yet to gain much traction in the United States. Recent regulatory proposals by the U.S. Environmental Protection Agency (EPA), limited in scope to new-build power plants, represent the only significant policy initiative intended to mandate diffusion of CCS technology. Phase I of this Project assessed barriers to CCS deployment as prioritized by the CCS community. That research concluded that there were four primary barriers: (1) cost, (2) lack of a carbon price, (3) liability, and (4) lack of a comprehensive regulatory regime. Phase II of this Project, as presented in this Report, assesses potential regulatory models for CCS and examines where those models address the hurdles to diffusing CCS technology identified in Phase I. It concludes (1) that a CCS-specific but flexible standard, such as a technology performance standard or a very particular type of market-based regulation, likely will promote CCS diffusion, and (2) that these policies cannot work alone, but rather, should be combined with other measures, such as liability limits and a comprehensive CCS regulatory regime.

  3. Open House 2016 Promotional Video | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Open House 2016 Promotional Video Share Description Argonne Open House 2016 Promotional Video Browse By - Any - General Argonne Information Energy -Energy efficiency --Vehicles ---Alternative fuels ---Automotive engineering ---Diesel ---Electric drive technology ---Hybrid & electric vehicles ---Hydrogen & fuel cells ---Internal combustion ---Powertrain research --Building design ---Construction --Manufacturing -Energy sources --Renewable energy ---Bioenergy ---Solar energy --Fossil fuels

  4. Bradbury Science Museum Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Museum Association Introducing Bradbury Science Museum Association The Bradbury Science Museum Association is a separate, not-for-profit entity envisioned to enhance the educational work of the museum. Contact Us thumbnail of Bradbury Science Museum (505) 667-4444 Email We welcome the Bradbury Science Museum Association as our partner in education. Join the BSMA and recieve all the benefits it provides. The pdf application is here. There are also Sponsorship and Business Partnerships available.

  5. Saline Infusion Markedly Reduces Impedance and Improves Efficacy of Pulmonary Radiofrequency Ablation

    SciTech Connect (OSTI)

    Gananadha, Sivakumar Morris, David Lawson

    2004-08-15

    Radiofrequency ablation (RFA) is a relatively new technique that has been investigated for the treatment of lung tumors. We evaluated for the first time the in vivo use of saline infusion during radiofrequency ablation of sheep lung. We performed RFA on 5 sheep using open and closed chest RFA and the RITA starburst XL and Xli probes using saline infusion with the Xli probe. The impedance and volume of ablation were compared. A total of 16 ablations were produced, 5 percutaneously and 11 open. The impedance during percutaneous and open RFA without saline infusion was 110 {+-} 16.2 and 183.3 {+-} 105.8 O, respectively. With the saline infusion the impedance was 71.3 {+-} 22O and 103.6 {+-} 37.5O. The effect of this was a significantly larger volume of ablation using the saline infusion during percutaneous RFA (90.6 {+-} 23 cm{sup 3} vs 10.47 {+-} 2.9 cm{sup 3}, p = 0.01) and open RFA (107.8 {+-} 25.8 cm{sup 3} vs 24.9 {+-} 19.3 cm{sup 3}, p = 0.0002). Saline infusion during RFA is associated with lower impedance, higher power delivery and larger lesion size.

  6. Fusion Power Associates Awards

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    fpa awards Fusion Power Associates Awards Fusion Power Associates is "a non-profit, tax-exempt research and educational foundation, providing information on the status of fusion development and other applications of plasma science and fusion research". The Association makes awards in four categories: Distinguished Career Awards, Leadership Awards, Excellence in Fusion Engineering, and Special Awards. Since 1987, Distinguished Career Awards have been presented "to individuals who

  7. Brookhaven Science Associates, LLC

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    3, 2015 Dr. Doon L. Gibbs Laboratory Director Brookhaven Science Associates, LLC Brookhaven National Laboratory 40 Brookhaven Avenue Upton, New York 11973-5000 WCO-2015-02 Dear Dr. Gibbs: The Office of Enterprise Assessments' Office of Enforcement completed its investigation into the facts and circumstances associated with the meteorological tower electrical shock event that occurred at the Brookhaven National Laboratory on November 12, 2014. Brookhaven Science Associates, LLC (BSA) documented

  8. National Hydropower Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hydropower Association - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power ...

  9. Weapons Program Associate Directors

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    integration we have achieved between the various components of the program," said Bret Knapp, Principal Associate Director for Weapons Programs. "They have both done an...

  10. Added Public Promotional Page! | OpenEI Community

    Open Energy Info (EERE)

    Added Public Promotional Page Home > Groups > Linked Open Data Workshop in Washington, D.C. Jweers's picture Submitted by Jweers(88) Contributor 27 September, 2012 - 18:10 Event...

  11. Demolitions Produce Recyclable Materials for Organization Promoting Economic Activity

    Broader source: Energy.gov [DOE]

    Demolitions have helped generate more than 8 million pounds of metal at the Piketon site for recycling, further promoting economic activity in the region thanks to the American Recovery and...

  12. Forest County Potawatomi Tribe Cuts Emissions, Promotes Green...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Forest County Potawatomi Tribe Cuts Emissions, Promotes Green Growth February 23, 2012 - ... In pursuit of its long-term energy goal of reducing its carbon footprint to zero, the ...

  13. Partnering with China to Promote Renewable Energy Deployment | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Partnering with China to Promote Renewable Energy Deployment Partnering with China to Promote Renewable Energy Deployment June 4, 2015 - 10:15am Addthis David Danielson, Assistant Secretary for Energy Efficiency and Renewable Energy, delivers keynote remarks at the fourth U.S.-China Renewable Energy Industries Forum in Washington, D.C. | <em>Photo by Josh Harmon</em> David Danielson, Assistant Secretary for Energy Efficiency and Renewable Energy, delivers keynote

  14. New institute promotes nuclear security | Y-12 National Security Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New institute promotes ... New institute promotes nuclear security Posted: February 11, 2013 - 3:27pm | Y-12 Report | Volume 9, Issue 2 | 2013 Disquieting headlines from recent news reports prompt an important question: Who has the breadth of knowledge and experience to help solve international nuclear challenges? "A lot of the keys for national nuclear security are right here in Oak Ridge - from processing uranium at Y-12 to developing medical isotopes at Oak Ridge National Laboratory to

  15. Project Profile: Influence of Novel Behavioral Strategies in Promoting the

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Diffusion of Solar Energy | Department of Energy Soft Costs » Project Profile: Influence of Novel Behavioral Strategies in Promoting the Diffusion of Solar Energy Project Profile: Influence of Novel Behavioral Strategies in Promoting the Diffusion of Solar Energy Logos of Yale University, New York University Stern School of Business, SmartPower, Clean Energy Finance and Investment Authority, and Solarize Connecticut. A line graph that highlights the social interactions for accelerating

  16. Secretary Chu Announces Progress on International Initiatives to Promote

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Clean Energy | Department of Energy International Initiatives to Promote Clean Energy Secretary Chu Announces Progress on International Initiatives to Promote Clean Energy April 8, 2011 - 12:00am Addthis Washington, D.C. - U.S. Energy Secretary Steven Chu this week joined with energy ministers and high-level representatives from more than 20 governments to announce renewed support for 11 international clean energy initiatives at the second Clean Energy Ministerial in Abu Dhabi, United Arab

  17. Secretary Chu Announces Winners of Student Competition to Promote

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy-Efficient Buildings | Department of Energy Winners of Student Competition to Promote Energy-Efficient Buildings Secretary Chu Announces Winners of Student Competition to Promote Energy-Efficient Buildings March 5, 2012 - 1:03pm Addthis WASHINGTON, D.C. -- As part of President Obama's all-of-the-above energy strategy, Energy Secretary Steven Chu today announced the winners of a competition that challenges collegiate students to develop and present real-world solutions to boost the

  18. Energy 101: Promoting Energy Education in the Nation's Colleges and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Universities | Department of Energy Energy 101: Promoting Energy Education in the Nation's Colleges and Universities Energy 101: Promoting Energy Education in the Nation's Colleges and Universities June 25, 2014 - 2:46pm Addthis The Energy 101 initiative supports ongoing efforts in energy education. The first webinar of the Energy 101 dialogue series will focus on energy in the classroom. | National Renewable Energy Laboratory photo The Energy 101 initiative supports ongoing efforts in

  19. NIST approach to Promoting US Innovation and Industrial Competitiveness |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy NIST approach to Promoting US Innovation and Industrial Competitiveness NIST approach to Promoting US Innovation and Industrial Competitiveness Breakout Session 1: New Developments and Hot Topics Session 1-A: Biomass and the U.S. Competitive Advantages for Manufacturing Clean Energy Products Gary Anderson, Economist, NIST b13_anderson_1-a.pdf (811.66 KB) More Documents & Publications Benefit-Cost Evaluation of U.S. DOE Investment in Energy Storage Technologies for

  20. New Field Laboratories and Related Research To Help Promote Environmentally

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Prudent Development of Unconventional Resources | Department of Energy Field Laboratories and Related Research To Help Promote Environmentally Prudent Development of Unconventional Resources New Field Laboratories and Related Research To Help Promote Environmentally Prudent Development of Unconventional Resources November 6, 2014 - 9:00am Addthis WASHINGTON, D.C. - Today, the Department of Energy announced the selection of three multiyear, field laboratories and six other multiyear research

  1. Promoting Combined Heat and Power (CHP) for Multifamily Properties, 2008 |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Promoting Combined Heat and Power (CHP) for Multifamily Properties, 2008 Promoting Combined Heat and Power (CHP) for Multifamily Properties, 2008 The U.S. Department of Housing and Urban Development (HUD) and the U.S. Department of Energy (DOE) and Oak Ridge National Laboratory (ORNL) developed preliminary feasibility (Level 1) screening software and enlisted the DOE CHP Regional Application Centers (RACs) to help run utility data and estimate paybacks. This paper

  2. Promoting NEPA Transparency and Public Engagement | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Promoting NEPA Transparency and Public Engagement Promoting NEPA Transparency and Public Engagement June 3, 2011 - 1:14pm Addthis "NEPA is, at its core, a transparency statute," said Katie Scharf, Council on Environmental Quality (CEQ) Deputy General Counsel, in opening a panel discussion on using information technology to support open government initiatives, engage the public, and add value to NEPA analysis. At the March 9, 2011, event - hosted by CEQ for Federal NEPA and legal staff

  3. Puget Sound Communities Promote Energy Efficiency | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Puget Sound Communities Promote Energy Efficiency Puget Sound Communities Promote Energy Efficiency April 2, 2010 - 4:50pm Addthis Lorelei Laird Writer, Energy Empowers What does this project do? Identifies wasted energy Frees up money for cash strapped programs in the area. Four organizations on Washington state's Kitsap Peninsula are joining forces to improve their energy efficiency. Led by the City of Bremerton, the largest participant city by population and energy needs, the four will hire

  4. DOE Announces $17 Million to Promote Greater Automobile Efficiency |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 7 Million to Promote Greater Automobile Efficiency DOE Announces $17 Million to Promote Greater Automobile Efficiency January 23, 2007 - 10:15am Addthis WASHINGTON, DC - U.S. Department of Energy (DOE) Assistant Secretary for Energy Efficiency and Renewable Energy Alexander Karsner today announced that DOE intends issue $17 million in solicitations to improve automobile efficiency and reduce the United States's dependence on foreign sources of oil. The funding will be

  5. Fischer–Tropsch Synthesis: Characterization Rb Promoted Iron Catalyst

    SciTech Connect (OSTI)

    Sarkar,A.; Jacobs, G.; Ji, Y.; Hamdeh, H.; Davis, B.

    2008-01-01

    Rubidium promoted iron Fischer-Tropsch synthesis (FTS) catalysts were prepared with two Rb/Fe atomic ratios (1.44/100 and 5/100) using rubidium nitrate and rubidium carbonate as rubidium precursors. Results of catalytic activity and deactivation studies in a CSTR revealed that rubidium promoted catalysts result in a steady conversion with a lower deactivation rate than that of the corresponding unpromoted catalyst although the initial activity of the promoted catalyst was almost half that of the unpromoted catalyst. Rubidium promotion results in lower methane production, and higher CO2, alkene and 1-alkene fraction in FTS products. M{umlt o}ssbauer spectroscopic measurements of CO activated and working catalyst samples indicated that the composition of the iron carbide phase formed after carbidization was -Fe5 C2 for both promoted and unpromoted catalysts. However, in the case of the rubidium promoted catalyst, '-Fe2.2C became the predominant carbidic phase as FTS continued and the overall catalyst composition remained carbidic in nature. In contrast, the carbide content of the unpromoted catalyst was found to decline very quickly as a function of synthesis time. Results of XANES and EXAFS measurements suggested that rubidium was present in the oxidized state and that the compound most prevalent in the active catalyst samples closely resembled that of rubidium carbonate.

  6. Associative list processing unit

    DOE Patents [OSTI]

    Hemmert, Karl Scott; Underwood, Keith D.

    2013-01-29

    An associative list processing unit and method comprising employing a plurality of prioritized cell blocks and permitting inserts to occur in a single clock cycle if all of the cell blocks are not full. Also, an associative list processing unit and method comprising employing a plurality of prioritized cell blocks and using a tree of prioritized multiplexers descending from the plurality of cell blocks.

  7. Histone deacetylase inhibitors epigenetically promote reparative events in primary dental pulp cells

    SciTech Connect (OSTI)

    Duncan, Henry F.; Smith, Anthony J.; Fleming, Garry J.P.; Cooper, Paul R.

    2013-06-10

    Application of histone deacetylase inhibitors (HDACi) to cells epigenetically alters their chromatin structure and induces transcriptional and cellular reparative events. This study investigated the application of two HDACi, valproic acid (VPA) and trichostatin A (TSA) on the induction of repair-associated responses in primary dental pulp cell (DPC) cultures. Flow cytometry demonstrated that TSA (100 nM, 400 nM) significantly increased cell viability. Neither HDACi was cytotoxic, although cell growth analysis revealed significant anti-proliferative effects at higher concentrations for VPA (>0.5 mM) and TSA (>50 nM). While high-content-analysis demonstrated that HDACi did not significantly induce caspase-3 or p21 activity, p53-expression was increased by VPA (3 mM, 5 mM) at 48 h. HDACi-exposure induced mineralization per cell dose-dependently to a plateau level (VPA-0.125 mM and TSA-25 nM) with accompanying increases in mineralization/dentinogenic-associated gene expression at 5 days (DMP-1, BMP-2/-4, Nestin) and 10 days (DSPP, BMP-2/-4). Both HDACis, at a range of concentrations, significantly stimulated osteopontin and BMP-2 protein expression at 10 and 14 days further supporting the ability of HDACi to promote differentiation. HDACi exert different effects on primary compared with transformed DPCs and promote mineralization and differentiation events without cytotoxic effects. These novel data now highlight the potential in restorative dentistry for applying low concentrations of HDACi in vital pulp treatment. -- Highlights: • Valproic acid and trichostatin A promoted mineralization in primary pulp cells. • Cell viability, apoptosis, caspase-3, p21 unaltered; p53 increased by valproic acid. • Trichostatin A increased cell viability at 24 h at selected concentrations. • Altered cell toxicity and differentiation between primary and transformed cells. • HDACi-induced the differentiation marker proteins osteopontin and BMP-2.

  8. Floyd Associates | Open Energy Information

    Open Energy Info (EERE)

    Associates Jump to: navigation, search Name: Floyd Associates Place: Beverly Hills, California Zip: 90210 Product: String representation "Floyd Associate ... GCC) countries." is...

  9. Isolated yeast promoter sequence and a method of regulated heterologous expression

    DOE Patents [OSTI]

    Gao, Johnway; Skeen, Rodney S.; Hooker, Brian S.; Anderson, Daniel B.

    2005-05-31

    The present invention provides the promoter clone discovery of a glucoamylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated glucoamylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

  10. Media Promotes SEP Certification and DOE eGuide to Help Facilities...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Technical Assistance Superior Energy Performance Media Promotes SEP Certification and DOE eGuide to Help Facilities Reduce Energy Use and Costs Media Promotes SEP Certification ...

  11. Associative list processing unit

    DOE Patents [OSTI]

    Hemmert, Karl Scott; Underwood, Keith D

    2014-04-01

    An associative list processing unit and method comprising employing a plurality of prioritized cell blocks and permitting inserts to occur in a single clock cycle if all of the cell blocks are not full.

  12. Association Loan Program

    Broader source: Energy.gov [DOE]

    The homeowners' association should submit a proposal to AHFC for preliminary approval. The forms to start this loan process are located on the program web site. Loan limits are determined on a...

  13. Postdoc Association Committees

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Committees Postdoc Association Committees Addressing the needs of Los Alamos Lab postdocs. Contact Postdoc Program Office Email Dynamic committees address needs of the Lab's postdocs The Los Alamos Postdoc Association (LAPA) created a number of dynamic committees to accomplish specific goals that address the needs of postdocs. As a goal is achieved, a committee may be phased out; as new needs are recognized then new committees will be created to help meet those needs. Career Committee The Career

  14. ALS Users' Association Charter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Users' Executive Committee ALS Users' Association Charter Print The purpose of the Advanced Light Source Users' Association (ALSUA) is to provide an organized framework for the interaction between those who use the Advanced Light Source (ALS) at the Lawrence Berkeley Laboratory (LBL) for their research and the ALS management, as well as to provide a channel for communication with other synchrotron radiation laboratories and, on suitable occasions, with federal agencies. The ALSUA, representing

  15. Oak Ridge Associated

    Office of Legacy Management (LM)

    ])\D Oak Ridge ,.(\\~ Associated ru~ Universities Post Office Box 11 7 Oak Ridge, Tennessee 37831-0117 October 21, 1986 Manpower Education, Research, and Training Division Mr. Edward G. Delaney, Director Division of Facility and Site Decommissioning Projects Office of Nuclear Energy U.S. Department of Energy Washington, DC 20545 Subject: VERIFICATION OF NIAGARA FALLS STORAGE SITE VICINITY PROPERTIES - 1983/1984 REMEDIAL ACTIONS Dear Mr. Delaney: Oak Ridge Associated Universities <ORAU) has

  16. Oak Ridge Associated Llniversities

    Office of Legacy Management (LM)

    ii!fil Prepared by Oak Ridge Associated Llniversities Prepared for U.S. Nuclear Regulatory Commission's Region I Office Supported by Safeguards dnd Materials Program Branch; Division of Quality Assurance, Safeguards, and Inspection Programs; Off ice of Inspection and r Enforcement I - CONFIRMATORY RADIOLOGICAL SURVEYS OF BUILDING 10 AND OUTSIDE AREAS ASSOCIATED WITH BUILDINGS 7 AND 8 WESTINGHOUSE NUCLEAR FUEL DIVISION CHESWICK, PENNSYLVANIA A. J. BOERNER Radiological Site Assessment Program

  17. ALS Users' Association Charter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Users' Association Charter Print The purpose of the Advanced Light Source Users' Association (ALSUA) is to provide an organized framework for the interaction between those who use the Advanced Light Source (ALS) at the Lawrence Berkeley Laboratory (LBL) for their research and the ALS management, as well as to provide a channel for communication with other synchrotron radiation laboratories and, on suitable occasions, with federal agencies. The ALSUA, representing the research workers, will be in

  18. ALS Users' Association Charter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Users' Association Charter Print The purpose of the Advanced Light Source Users' Association (ALSUA) is to provide an organized framework for the interaction between those who use the Advanced Light Source (ALS) at the Lawrence Berkeley Laboratory (LBL) for their research and the ALS management, as well as to provide a channel for communication with other synchrotron radiation laboratories and, on suitable occasions, with federal agencies. The ALSUA, representing the research workers, will be in

  19. ALS Users' Association Charter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ALS Users' Association Charter Print The purpose of the Advanced Light Source Users' Association (ALSUA) is to provide an organized framework for the interaction between those who use the Advanced Light Source (ALS) at the Lawrence Berkeley Laboratory (LBL) for their research and the ALS management, as well as to provide a channel for communication with other synchrotron radiation laboratories and, on suitable occasions, with federal agencies. The ALSUA, representing the research workers, will

  20. Bradbury Science Museum Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bradbury Science Museum Association 108 Central Park Square #208 Los Alamos, NM 87544 Dear Los Alamos National Laboratory Employee, member of the Los Alamos Community, or other friend of the Bradbury Science Museum, I am writing today to tell you about an exciting new opportunity! As of May 1, 2016, we are launching the Bradbury Science Museum Association (BSMA). Our purpose is to amplify the Bradbury Science Museum's (BSM) mission and visitor experience as its official nonprofit partner,

  1. Organizations and associations serving the Waste-To-energy industry

    SciTech Connect (OSTI)

    Not Available

    1998-12-01

    Professional organizations can provide leadership in disseminating information and answering questions about, and in providing support for, the industry. Eleven such organizations and association that directly, or in part, promote or provide technical assistance in the waste-to-energy field are listed and described briefly. Some actively lobby on waste-to-energy issues. Some provide useful publications and newsletters for those interested in keeping up with changes in the field.

  2. The case for reasonable incentives to promote independent transmission companies

    SciTech Connect (OSTI)

    Angle, S.; Carpentier, D.A.; Farinella, M.

    1999-09-01

    As part of its restructuring efforts in the electric utility industry, the Federal Energy Regulatory Commission (FERC) has encouraged voluntary corporate unbundling in an effort to promote a competitive electric market, recently proposing to define the minimal and desired characteristics of a regional transmission organization (RTO). The promotion of competition requires certain features in a transmission system, which include inter alia, (1) independent operation; (2) efficient pricing of congestion; (3) congestion management; (4) provision of ancillary services; (5) elimination of pancaked transmission rates; (6) appropriate transmission pricing; and (7) price transparency. A promising type of corporate unbundling that can provide these features is an independent transmission company (ITC).

  3. GE Showcases Industrial Internet Innovations and Promotes Win-Win

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cooperation at 2015 TECHfest | GE Global Research Showcases Industrial Internet Innovations and Promotes Win-Win Cooperation at 2015 TECHfest Click to email this to a friend (Opens in new window) Share on Facebook (Opens in new window) Click to share (Opens in new window) Click to share on LinkedIn (Opens in new window) Click to share on Tumblr (Opens in new window) GE Showcases Industrial Internet Innovations and Promotes Win-Win Cooperation at 2015 TECHfest GE China Technology Center teams

  4. Scaffold architecture and fibrin gels promote meniscal cell proliferation

    SciTech Connect (OSTI)

    Pawelec, K. M. E-mail: jw626@cam.ac.uk; Best, S. M.; Cameron, R. E.; Wardale, R. J. E-mail: jw626@cam.ac.uk

    2015-01-01

    Stability of the knee relies on the meniscus, a complex connective tissue with poor healing ability. Current meniscal tissue engineering is inadequate, as the signals for increasing meniscal cell proliferation have not been established. In this study, collagen scaffold structure, isotropic or aligned, and fibrin gel addition were tested. Metabolic activity was promoted by fibrin addition. Cellular proliferation, however, was significantly increased by both aligned architectures and fibrin addition. None of the constructs impaired collagen type I production or triggered adverse inflammatory responses. It was demonstrated that both fibrin gel addition and optimized scaffold architecture effectively promote meniscal cell proliferation.

  5. Faster Tracks for Particle Accelerators Promoted by ODU Physicists (Inside

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ODU) | Jefferson Lab Faster Tracks for Particle Accelerators Promoted by ODU Physicists (Inside ODU) External Link: http://ww2.odu.edu/ao/ia/insideodu/20120426/topstory2.html By jlab_admin on Thu, 2012-04-2

  6. Savannah River Site Promotes Sharing Technology Innovation Across DOE Complex

    Broader source: Energy.gov [DOE]

    AIKEN, S.C. – An innovative data collection technology for environmental compliance sampling developed and used by the Savannah River Site is now being deployed at the Portsmouth Gaseous Diffusion Plant in Piketon, Ohio to increase the efficiency of cleanup operations as EM continues to promote sharing lessons learned and new technologies across DOE sites.

  7. United States, Australia, and Iceland to Promote Geothermal Energy

    Broader source: Energy.gov [DOE]

    The United States, Australia, and Iceland signed a charter on August 28 to launch the International Partnership for Geothermal Technology (IPGT), which will foster and promote cutting-edge geothermal technologies and help address energy security and address global climate change.

  8. Cysteine dioxygenase type 1 promotes adipogenesis via interaction with peroxisome proliferator-activated receptor gamma

    SciTech Connect (OSTI)

    Deng, Peng; Chen, Yi; Ji, Ning; Lin, Yunfeng; Yuan, Quan; Ye, Ling; Chen, Qianming

    2015-02-27

    Mammalian cysteine dioxygenase type 1 (CDO1) is an essential enzyme for taurine biosynthesis and the biodegradation of toxic cysteine. As previously suggested, Cdo1 may be a marker of liposarcoma progression and adipogenic differentiation, but the role of Cdo1 in adipogenesis has yet been reported. In this study, we found that the expression of Cdo1 is dramatically elevated during adipogenic differentiation of 3T3-L1 pre-adipocytes and mouse bone marrow-derived mesenchymal stem cells (mBMSCs). Conversely, knockdown of Cdo1 inhibited expression of adipogenic specific genes and lipid droplet formation in 3T3-L1 cells and mBMSCs. Mechanistically, we found Cdo1 interacted with Pparγ in response to adipogenic stimulus. Further, depletion of Cdo1 reduced the recruitment of Pparγ to the promoters of C/EBPα and Fabp4. Collectively, our finding indicates that Cdo1 may be a co-activator of Pparγ in adipogenesis, and may contribute to the development of disease associated with excessive adipose tissue. - Highlights: • Cdo1expression is highly up-regulated during adipogenic differentiation of 3T3-L1 and mBMSCs. • Depletion of Cdo1 inhibited expression of adipogenic specific genes and lipid droplet formation. • Cdo1interacts with Pparγ during adipogenesis. • Knockdown of Cdo1 inhibited Pparγ binding to the promoters of C/EBPα and Fabp4.

  9. Promoting high efficiency residential HVAC equipment: Lessons learned from leading utility programs

    SciTech Connect (OSTI)

    Neme, C.; Peters, J.; Rouleau, D.

    1998-07-01

    The Consortium for Energy Efficiency recently sponsored a study of leading electric utility efforts to promote high efficiency residential HVAC equipment. Given growing concerns from some utilities about the level of expenditures associated with rebate programs, special emphasis was placed on assessing the success of financing and other non-rebate options for promoting efficiency. Emphasis was also placed on review of efforts--rebate or otherwise--to push the market to very high levels of efficiency (i.e., SEER 13). This paper presents the results of the study. It includes discussion of key lessons from the utility programs analyzed. It also examines program participation rates and other potential indicators of market impacts. One notable conclusion is that several utility programs have pushed market shares for SEER 12 equipment to about 50% (the national average is less than 20%). At least one utility program has achieved a 50% market share for SEER 13 equipment (the national average is less than 3%). In general, financing does not appear to have as broad an appeal as consumer rebates. However, one unique utility program which combines the other of customer financing with modest incentives to contractors--in the form of frequent seller points that can be redeemed for advertising, technician training, travel and other merchandise--offers some promise that high participation rates can be achieved without customer rebates.

  10. Service Academies Research Associates

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Internships » SARA Service Academies Research Associates Military academies come to Los Alamos for a summer of science. October 9, 2015 West Point cadets at the National Security Science Building at Los Alamos National Laboratory. West Point cadets at the National Security Science Building at Los Alamos National Laboratory. Contact Jonathan S. Ventura Email SARA interns use science and engineering to meet challenges to national security. SARA: not what you'd expect Through the Service Academies

  11. National Electrical Manufacturers Association

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    July 24, 2014 VIA EMAIL TO: Regulatory.Review@hq.doe.gov Steven Croley, General Counsel Office of the General Counsel U.S. Department of Energy 1000 Independence Avenue SW., Washington, DC 20585 NEMA Comments on DOE Reducing Regulatory Burden RFI 79 Fed.Reg. 28518 (July 3, 2014) Dear Mr. Croley, The National Electrical Manufacturers Association (NEMA) thanks you for the opportunity to provide comments on the Department of Energy's efforts to make its regulatory program more effective and less

  12. Service Academies Research Associates

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Internships » SARA » SARA Service Academies Research Associates (SARA) The academies come to Los Alamos for a summer of science. Contact Jonathan S. Ventura Email application Program 2015-2016 Application Your Name Date of Birth SSN Home Address Phone Email Address at the Academy Academy Approver's Name Academy Approver's Phone Academy Approver's Email GPA (requires minimum 3.0) Academy Major Foreign Language(s) and Proficiency Preferred Work Environment (lab or office space) Dates Available

  13. Mananook Associates | Open Energy Information

    Open Energy Info (EERE)

    Mananook Associates Jump to: navigation, search Name: Mananook Associates Address: PO Box 69 Place: Perry Zip: 4667 Region: United States Sector: Marine and Hydrokinetic Phone...

  14. Broin Associates | Open Energy Information

    Open Energy Info (EERE)

    search Name: Broin & Associates Place: Sioux Falls, South Dakota Zip: 57104 Product: Bioethanol plant designer, engineering and construction firm References: Broin & Associates1...

  15. Downregulated TIPE2 is associated with poor prognosis and promotes cell proliferation in non-small cell lung cancer

    SciTech Connect (OSTI)

    Li, Yuexia; Li, Xiaohui; Liu, Gang; Sun, Rongqing; Wang, Lirui; Wang, Jing; Wang, Hongmin

    2015-01-30

    Highlights: • TIPE2 is down-regulated in NSCLC tissues. • TIPE2 inhibits NSCLC cell proliferation, colony formation and invasion. • TIPE2 reduces the anti-apoptotic Bcl-XL protein and mesenchymal marker N-cadherin expression. - Abstract: The present study aims to investigate the expression pattern of TIPE2 protein and its clinical significance in human non-small cell lung cancer (NSCLC). We investigated the expression levels of TIPE2 in 96 NSCLC tumor samples by immunohistochemistry and then analyzed its clinical significance. Furthermore, the role of TIPE2 on the biological properties of the NSCLC cell line H1299 and A549 was experimentally tested in vitro and in vivo. We found that the expression level of TIPE2 was significantly higher in normal lung tissues compared with NSCLC tissues (P < 0.001), and TIPE2 downregulation was significantly correlated with advanced TNM stage (P = 0.006). TIPE2 expression was lower in lung cancer cell lines than normal bronchial cell line HBE. Transfection of TIPE2 plasmid was performed in H1299 and A549 cells. TIPE2 overexpression inhibited lung cancer cell proliferation, colony formation and cell invasive in vitro, and prevented lung tumor growth in vivo. In addition, TIPE2 transfection reduced the anti-apoptotic Bcl-XL protein and mesenchymal marker N-cadherin expression. Taken together, our results demonstrate that TIPE2 might serve as a tumor suppressor in NSCLC progression.

  16. Material and method for promoting the growth of anaerobic bacteria

    DOE Patents [OSTI]

    Adler, Howard I.

    1984-01-01

    A material and method for promoting the growth of anaerobic bacteria which includes a nutrient media containing a hydrogen donor and sterile membrane fragments of bacteria having an electron transfer system which reduces oxygen to water. Dissolved oxygen in the medium is removed by adding the sterile membrane fragments to the nutrient medium and holding the medium at a temperature of about 10.degree. to about 60.degree. C. until the dissolved oxygen is removed.

  17. Material and method for promoting the growth of anaerobic bacteria

    DOE Patents [OSTI]

    Adler, H.I.

    1984-10-09

    A material and method is disclosed for promoting the growth of anaerobic bacteria which includes a nutrient media containing a hydrogen donor and sterile membrane fragments of bacteria having an electron transfer system which reduces oxygen to water. Dissolved oxygen in the medium is removed by adding the sterile membrane fragments to the nutrient medium and holding the medium at a temperature of about 10 to about 60 C until the dissolved oxygen is removed. No Drawings

  18. NNSA Deputy promotes collaboration on global deterrence at USSTRATCOM

    National Nuclear Security Administration (NNSA)

    deterrence symposium | National Nuclear Security Administration | (NNSA) Deputy promotes collaboration on global deterrence at USSTRATCOM deterrence symposium Wednesday, August 17, 2016 - 2:57pm The first panel of the symposium, focused on ensuring the credibility of nuclear deterrence and assurance capabilities, moderated by Madelyn Creedon. Last month more than 650 experts representing government, military, and academic institutions gathered for the U.S. Strategic Command's (USSTRATCOM)

  19. Switchgrass ubiquitin promoter (PVUBI2) and uses thereof

    DOE Patents [OSTI]

    Stewart, C. Neal; Mann, David George James

    2013-12-10

    The subject application provides polynucleotides, compositions thereof and methods for regulating gene expression in a plant. Polynucleotides disclosed herein comprise novel sequences for a promoter isolated from Panicum virgatum (switchgrass) that initiates transcription of an operably linked nucleotide sequence. Thus, various embodiments of the invention comprise the nucleotide sequence of SEQ ID NO: 2 or fragments thereof comprising nucleotides 1 to 692 of SEQ ID NO: 2 that are capable of driving the expression of an operably linked nucleic acid sequence.

  20. Antonya Sanders-Promoting nanoscience integration through outreach

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Antonya Sanders Antonya Sanders-Promoting nanoscience integration through outreach Bringing together university faculty, students, researchers and other Laboratory scientists to explore nanoscale science. March 17, 2014 Antonya Sanders Sanders' focus on serving others began early on: she lived in England and traveled the world to find ways to help others, including aiding Bosnian refugees. Sanders leads communication and outreach for the Lab's Center for Integrated Nanotechnologies (CINT), a

  1. Sandia, UNM Form Alliance to Promote Research, Recruiting

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    UNM Form Alliance to Promote Research, Recruiting - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Energy Defense Waste

  2. Structure of a bacterial RNA polymerase holoenzyme open promoter complex

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bae, Brian; Feklistov, Andrey; Lass-Napiorkowska, Agnieszka; Landick, Robert; Darst, Seth A.

    2015-09-08

    Initiation of transcription is a primary means for controlling gene expression. In bacteria, the RNA polymerase (RNAP) holoenzyme binds and unwinds promoter DNA, forming the transcription bubble of the open promoter complex (RPo). We have determined crystal structures, refined to 4.14 Å-resolution, of RPo containing Thermus aquaticus RNAP holoenzyme and promoter DNA that includes the full transcription bubble. The structures, combined with biochemical analyses, reveal key features supporting the formation and maintenance of the double-strand/single-strand DNA junction at the upstream edge of the -10 element where bubble formation initiates. The results also reveal RNAP interactions with duplex DNA just upstreammore » of the -10 element and potential protein/DNA interactions that direct the DNA template strand into the RNAP active site. Additionally a RNA primer to yield a 4 base-pair post-translocated RNA:DNA hybrid mimics an initially transcribing complex at the point where steric clash initiates abortive initiation and σA dissociation.« less

  3. Structure of a bacterial RNA polymerase holoenzyme open promoter complex

    SciTech Connect (OSTI)

    Bae, Brian; Feklistov, Andrey; Lass-Napiorkowska, Agnieszka; Landick, Robert; Darst, Seth A.

    2015-09-08

    Initiation of transcription is a primary means for controlling gene expression. In bacteria, the RNA polymerase (RNAP) holoenzyme binds and unwinds promoter DNA, forming the transcription bubble of the open promoter complex (RPo). We have determined crystal structures, refined to 4.14 Å-resolution, of RPo containing Thermus aquaticus RNAP holoenzyme and promoter DNA that includes the full transcription bubble. The structures, combined with biochemical analyses, reveal key features supporting the formation and maintenance of the double-strand/single-strand DNA junction at the upstream edge of the -10 element where bubble formation initiates. The results also reveal RNAP interactions with duplex DNA just upstream of the -10 element and potential protein/DNA interactions that direct the DNA template strand into the RNAP active site. Additionally a RNA primer to yield a 4 base-pair post-translocated RNA:DNA hybrid mimics an initially transcribing complex at the point where steric clash initiates abortive initiation and σA dissociation.

  4. TUNL Associated Theses

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    M.S. and M.A. Degrees Theses Associated Degrees Theses Browse by year 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 2012 Wenzhong Wu, Feedback Systems for Control of Coupled-bunch Instabilities in the Duke Storage Ring. Duke University, May 31, 2012, Supervisor: Y.K. Wu. Download PDF (12 MB) 2011 Botao Jia, Study of Storage Ring Free-Electron Laser Using Experimental and Simulation Approaches. Duke University, July 2011, Supervisor: Y.K. Wu.

  5. Oak Ridge Associated

    Office of Legacy Management (LM)

    2012 IL.06 *128 Oak Ridge Associated Post Of/ICE: 80 '17 Unl e Sllles Oa d. )Cp€ T nness £: 37 1 *01 '7 '-1.\0.-»"--" 10. June 14, 1989 Mr. Andrew Wallo ruSRAP/Surplus Facilities Group Division of Facili y & Site Decommissioning Projects Office of Nuclear Energy U.S. D~partment of Energy Washington, D.C. 20545 Subject: LETTER REPORT - VERIFIC~TION ACTIVITIES AT UNIVERSITY OF CHICAGO Dear Mr. Wallo: Enclosed is the report for the recent ORAU verification activities involving

  6. associates | National Nuclear Security Administration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    associates Smithsonian Associates Study Tour visits Y-12 The Smithsonian Associates Study Tour. This year marks the second year that the Smithsonian Institution has featured an Oak Ridge tour for its annual Associates Study Tour. Forty-three teachers and two tour leaders traveled from Washington to Oak Ridge for the three-day workshop packed with history...

  7. Northeast Sustainable Energy Association NESEA | Open Energy...

    Open Energy Info (EERE)

    organization focused on promoting the understanding, development, and adoption of energy conservation and non-polluting, renewable energy technologies. Coordinates:...

  8. Promotion of Rural Renewable Energy in Western China | Open Energy...

    Open Energy Info (EERE)

    Energy in Western China Place: Beijing Municipality, China Zip: 100026 Sector: Bioenergy Product: A programme launched by China Association of Rural Energy Industry (CAREI)...

  9. International District Energy Association

    Broader source: Energy.gov [DOE]

    Since its formation in 1909, the International District Energy Association (IDEA) has served as a principal industry advocate and management resource for owners, operators, developers, and suppliers of district heating and cooling systems in cities, campuses, bases, and healthcare facilities. Today, with over 1,400 members in 26 countries, IDEA continues to organize high-quality technical conferences that inform, connect, and advance the industry toward higher energy efficiency and lower carbon emissions through innovation and investment in scalable sustainable solutions. With the support of DOE, IDEA performs industry research and market analysis to foster high impact projects and help transform the U.S. energy industry. IDEA was an active participant in the original Vision and Roadmap process and has continued to partner with DOE on combined heat and power (CHP) efforts across the country.

  10. Oak Ridge Associ Universities

    Office of Legacy Management (LM)

    ir.\ "'t-"' , i 'Prepared by Oak Ridge Associ Universities Prepared for Division of Remedial Action Proiects 'U.S. Department of Energy 5 : ! l :;"i\ r l!! ,iri$, t . r ' i , , . 1 . E".:r- i{$, i. 'ii idi 1, . :{. I i:li C O M P R E H E N S I V E R A D I O L O G I C A L S U R V E Y O F F - S I T E P R O P E R T Y W N I A G A R A F A L L S S T O R A G E S I T E LEWlsToN, NEW YORK J . D . B E R G E R Radiol-oglcal Site Assessment Program Manpower Education, Research, and

  11. Oak Ridge Associated

    Office of Legacy Management (LM)

    l/s1 Prepared by Oak Ridge Associated 'Universities Prepared for Division of Remedial Action Froiects ilJ..S. Department of Energy N( , /7 C O M P R E H E N S I V E R A D I O L O G I C A L S U R V E Y O F F . S I T E P R O P E R T Y F N I A G A R A F A L L S S T O R A G E S I T E L E W I S T O N , N E W Y O R K J. D. BERGER Radiologieal Site Assessment Program . Manpower Education, Research, and Training Division FINAL REPORT February 1984 COMPREHENSIVE RADIOLOGICAI SURVEY OFF-SITE PROPERTY F

  12. Secretary Chu to Travel to India and China to Promote Clean Energy...

    Energy Savers [EERE]

    India and China to Promote Clean Energy Partnerships Secretary Chu to Travel to India and China to Promote Clean Energy Partnerships November 10, 2009 - 12:00am Addthis Washington, ...

  13. Mexico-GTZ Support for the Programme to Promote Solar Water Heating...

    Open Energy Info (EERE)

    Support for the Programme to Promote Solar Water Heating Jump to: navigation, search Logo: Mexico-GTZ Support for the Programme to Promote Solar Water Heating Name Mexico-GTZ...

  14. Establishing and Promoting a Culture of Safety in Chemistry Laboratory Research

    SciTech Connect (OSTI)

    Fryberger, Teresa

    2014-12-23

    Final technical/scientific report for the project, Establishing and Promoting a Culture of Safety in Chemistry Laboratory Research.

  15. Gateways to the FANTOM5 promoter level mammalian expression atlas

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lizio, Marina; Harshbarger, Jayson; Shimoji, Hisashi; Severin, Jessica; Kasukawa, Takeya; Sahin, Serkan; Abugessaisa, Imad; Fukuda, Shiro; Hori, Fumi; Ishikawa-Kato, Sachi; et al

    2015-01-05

    The FANTOM5 project investigates transcription initiation activities in more than 1,000 human and mouse primary cells, cell lines and tissues using CAGE. Based on manual curation of sample information and development of an ontology for sample classification, we assemble the resulting data into a centralized data resource (http://fantom.gsc.riken.jp/5/). In conclusion, this resource contains web-based tools and data-access points for the research community to search and extract data related to samples, genes, promoter activities, transcription factors and enhancers across the FANTOM5 atlas.

  16. E2F1 promote the aggressiveness of human colorectal cancer by activating the ribonucleotide reductase small subunit M2

    SciTech Connect (OSTI)

    Fang, Zejun; Gong, Chaoju; Liu, Hong; Zhang, Xiaomin; Mei, Lingming; Song, Mintao; Qiu, Lanlan; Luo, Shuchai; Zhu, Zhihua; Zhang, Ronghui; Gu, Hongqian; Chen, Xiang

    2015-08-21

    As the ribonucleotide reductase small subunit, the high expression of ribonucleotide reductase small subunit M2 (RRM2) induces cancer and contributes to tumor growth and invasion. In several colorectal cancer (CRC) cell lines, we found that the expression levels of RRM2 were closely related to the transcription factor E2F1. Mechanistic studies were conducted to determine the molecular basis. Ectopic overexpression of E2F1 promoted RRM2 transactivation while knockdown of E2F1 reduced the levels of RRM2 mRNA and protein. To further investigate the roles of RRM2 which was activated by E2F1 in CRC, CCK-8 assay and EdU incorporation assay were performed. Overexpression of E2F1 promoted cell proliferation in CRC cells, which was blocked by RRM2 knockdown attenuation. In the migration and invasion tests, overexpression of E2F1 enhanced the migration and invasion of CRC cells which was abrogated by silencing RRM2. Besides, overexpression of RRM2 reversed the effects of E2F1 knockdown partially in CRC cells. Examination of clinical CRC specimens demonstrated that both RRM2 and E2F1 were elevated in most cancer tissues compared to the paired normal tissues. Further analysis showed that the protein expression levels of E2F1 and RRM2 were parallel with each other and positively correlated with lymph node metastasis (LNM), TNM stage and distant metastasis. Consistently, the patients with low E2F1 and RRM2 levels have a better prognosis than those with high levels. Therefore, we suggest that E2F1 can promote CRC proliferation, migration, invasion and metastasis by regulating RRM2 transactivation. Understanding the role of E2F1 in activating RRM2 transcription will help to explain the relationship between E2F1 and RRM2 in CRC and provide a novel predictive marker for diagnosis and prognosis of the disease. - Highlights: • E2F1 promotes RRM2 transactivation in CRC cells. • E2F1 promotes the proliferation of CRC cells by activating RRM2. • E2F1 promotes the migration and

  17. Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression

    DOE Patents [OSTI]

    Gao, Johnway [Richland, WA; Skeen, Rodney S [Pendleton, OR; Hooker, Brian S [Kennewick, WA; Anderson, Daniel B [Pasco, WA

    2003-04-01

    The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

  18. The soil microbiome influences grapevine-associated microbiota

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zarraonaindia, Iratxe; Owens, Sarah M.; Weisenhorn, Pamela; West, Kristin; Hampton-Marcell, Jarrad; Lax, Simon; Bokulich, Nicholas A.; Mills, David A.; Martin, Gilles; Taghavi, Safiyh; et al

    2015-03-24

    Grapevine is a well-studied, economically relevant crop, whose associated bacteria could influence its organoleptic properties. In this study, the spatial and temporal dynamics of the bacterial communities associated with grapevine organs (leaves, flowers, grapes, and roots) and soils were characterized over two growing seasons to determine the influence of vine cultivar, edaphic parameters, vine developmental stage (dormancy, flowering, preharvest), and vineyard. Belowground bacterial communities differed significantly from those aboveground, and yet the communities associated with leaves, flowers, and grapes shared a greater proportion of taxa with soil communities than with each other, suggesting that soil may serve as a bacterialmore » reservoir. A subset of soil microorganisms, including root colonizers significantly enriched in plant growth-promoting bacteria and related functional genes, were selected by the grapevine. In addition to plant selective pressure, the structure of soil and root microbiota was significantly influenced by soil pH and C:N ratio, and changes in leaf- and grape-associated microbiota were correlated with soil carbon and showed interannual variation even at small spatial scales. Diazotrophic bacteria, e.g., Rhizobiaceae and Bradyrhizobium spp., were significantly more abundant in soil samples and root samples of specific vineyards. Vine-associated microbial assemblages were influenced by myriad factors that shape their composition and structure, but the majority of organ-associated taxa originated in the soil, and their distribution reflected the influence of highly localized biogeographic factors and vineyard management.« less

  19. The soil microbiome influences grapevine-associated microbiota

    SciTech Connect (OSTI)

    Zarraonaindia, Iratxe; Owens, Sarah M.; Weisenhorn, Pamela; West, Kristin; Hampton-Marcell, Jarrad; Lax, Simon; Bokulich, Nicholas A.; Mills, David A.; Martin, Gilles; Taghavi, Safiyh; van der Lelie, Daniel; Gilbert, Jack A.

    2015-03-24

    Grapevine is a well-studied, economically relevant crop, whose associated bacteria could influence its organoleptic properties. In this study, the spatial and temporal dynamics of the bacterial communities associated with grapevine organs (leaves, flowers, grapes, and roots) and soils were characterized over two growing seasons to determine the influence of vine cultivar, edaphic parameters, vine developmental stage (dormancy, flowering, preharvest), and vineyard. Belowground bacterial communities differed significantly from those aboveground, and yet the communities associated with leaves, flowers, and grapes shared a greater proportion of taxa with soil communities than with each other, suggesting that soil may serve as a bacterial reservoir. A subset of soil microorganisms, including root colonizers significantly enriched in plant growth-promoting bacteria and related functional genes, were selected by the grapevine. In addition to plant selective pressure, the structure of soil and root microbiota was significantly influenced by soil pH and C:N ratio, and changes in leaf- and grape-associated microbiota were correlated with soil carbon and showed interannual variation even at small spatial scales. Diazotrophic bacteria, e.g., Rhizobiaceae and Bradyrhizobium spp., were significantly more abundant in soil samples and root samples of specific vineyards. Vine-associated microbial assemblages were influenced by myriad factors that shape their composition and structure, but the majority of organ-associated taxa originated in the soil, and their distribution reflected the influence of highly localized biogeographic factors and vineyard management.

  20. Environmental Management Associates | Open Energy Information

    Open Energy Info (EERE)

    Environmental Management Associates (Redirected from EMA Associates) Jump to: navigation, search Name: Environmental Management Associates Abbreviation: EMA Website:...

  1. Strategies for promoting renewables in a new electric industry

    SciTech Connect (OSTI)

    Driver, B.

    1996-12-31

    This paper describes strategies for promoting renewable resources in an era characterized by competitive pressures in the electric industry. It begins with a background section to describe the perspective from which I am writing and the nature of the pressures confronting renewables in 1996. Then, the paper turns to a discussion of the regulatory and other options to promote renewables in this environment. The major conclusion of the paper is that there is no {open_quotes}magic bullet{close_quotes} to guide the development of renewables through the developing competitive era within the electric industry. Indeed, it appears that the job can get done only through a combination of different measures at all levels of government. The author believes that among the most effective measures are likely to be: a national renewable resources generation standard; conditions attached to restructuring events; regional interstate compacts; regional risk-sharing consortia supported by federal and state tax and fiscal policy; and state {open_quotes}systems benefits charges;{close_quotes}

  2. Low severity coal liquefaction promoted by cyclic olefins

    SciTech Connect (OSTI)

    Curtis, C.W.

    1992-07-27

    Low severity coal liquefaction allows for solubilization of coal with reduced gas make. These lower severity conditions may result in some selective bond rupture. Promotion of coal solubilization through hydrogen transfer using highly active and effective hydrogen donors is the objective of this study. The highly effective donors being tested are cyclic olefins. Representative cyclic olefins are isotetralin, which is 1,4,5,8-tetrahydronaphthalene, and 1,4,5,8,9,10-hexahydroanthracene. These compounds are hydroaromatics without aromatic rings and have been shown to be highly effective donors. The objective of the work performed in this study during this quarter was to evaluate reaction parameters for low severity liquefaction reactions using the cyclic olefin, hexahydroanthracene, and the aromatic, anthracene. These model compounds were reacted under a variety of conditions to evaluate their reactivity without coal. The reactions were performed under both thermal and catalytic conditions. Finely divided catalysts from different molybdenum precursors were used to determine their activity in promoting hydrogenation and hydrogen transfer at low severity conditions. The catalysts used were Molyvan L, sulfurized oxymolybdenum dithiocarbamate, molybdenum naphthenate, and Molyvan 822, organo molybdenum dithiocarbamate.

  3. Activation of type 2 cannabinoid receptors (CB2R) promotes fatty acid oxidation through the SIRT1/PGC-1? pathway

    SciTech Connect (OSTI)

    Zheng, Xuqin; Sun, Tao; Wang, Xiaodong

    2013-07-05

    Highlights: TC, a CB2R specific agonist, stimulates SIRT1 activity by PKA/CREB pathway. TC promotes PGC-1? transcriptional activity by increasing its deacetylation. TC increases the expression of genes linked to FAO and promotes the rate of FAO. The effects of TC in FAO are dependent on CB2R. Suggesting CB2R as a target to treat diseases with lipid dysregulation. -- Abstract: Abnormal fatty acid oxidation has been associated with obesity and type 2 diabetes. At the transcriptional level, peroxisome proliferator-activated receptor-gamma coactivator 1? (PGC-1?) has been reported to strongly increase the ability of hormone nuclear receptors PPAR? and ERR? to drive transcription of fatty acid oxidation enzymes. In this study, we report that a specific agonist of the type 2 cannabinoid receptor (CB2R) can lead to fatty acid oxidation through the PGC-1? pathway. We have found that CB2R is expressed in differentiated C2C12 myotubes, and that use of the specific agonist trans-caryophyllene (TC) stimulates sirtuin 1 (SIRT1) deacetylase activity by increasing the phosphorylation of cAMP response element-binding protein (CREB), thus leading to increased levels of PGC-1? deacetylation. This use of TC treatment increases the expression of genes linked to the fatty acid oxidation pathway in a SIRT1/PGC-1?-dependent mechanism and also drastically accelerates the rate of complete fatty acid oxidation in C2C12 myotubes, neither of which occur when CB2R mRNA is knocked down using siRNA. These results reveal that activation of CB2R by a selective agonist promotes lipid oxidation through a signaling/transcriptional pathway. Our findings imply that pharmacological manipulation of CB2R may provide therapeutic possibilities to treat metabolic diseases associated with lipid dysregulation.

  4. A neural network system for prediction of RNA polymerase II promoters

    SciTech Connect (OSTI)

    Matis, S.; Shah, M.; Mural, R.; Uberbacher, E.

    1994-12-31

    One of the most difficult problems in the analysis of eucaryotic genes is the detection of RNA polymerase II promoter regions. Although promoter regions vary in the primary DNA sequence, a basic group of core promoter elements has been suggested in the literature. Many human promoter sequences contain a TATAA sequence element at approximately 30 bases upstream of the cap site (transcription start site). Other elements are the GC box which binds SPA and upregulates transcription, the CAAT box, and the ATG initiator codon. To characterize promoters, we constructed frequency matrices for each element using experimentally mapped human promoter regions. Additionally, we constructed histograms for the distances separating the various elements. We then used a neural network to combine these informational elements. The output of the neural network is then processed using a set of expert rules which depend on GRAIL`s ability to find exons in anonymous DNA. This improves the selectivity of promoter detection and reduces the false positive rate.

  5. Encapsulant materials and associated devices

    DOE Patents [OSTI]

    Kempe, Michael D; Thapa, Prem

    2012-05-22

    Compositions suitable for use as encapsulants are described. The inventive compositions include a high molecular weight polymeric material, a curing agent, an inorganic compound, and a coupling agent. Optional elements include adhesion promoting agents, colorants, antioxidants, and UV absorbers. The compositions have desirable diffusivity properties, making them suitable for use in devices in which a substantial blocking of moisture ingress is desired, such as photovoltaic (PV) modules.

  6. Encapsulant materials and associated devices

    DOE Patents [OSTI]

    Kempe, Michael D; Thapa, Prem

    2011-03-08

    Compositions suitable for use as encapsulants are described. The inventive compositions include a high molecular weight polymeric material, a curing agent, an inorganic compound, and a coupling agent. Optional elements include adhesion promoting agents, colorants, antioxidants, and UV absorbers. The compositions have desirable diffusivity properties, making them suitable for use in devices in which a substantial blocking of moisture ingress is desired, such as photovoltaic (PV) modules.

  7. Harmine promotes osteoblast differentiation through bone morphogenetic protein signaling

    SciTech Connect (OSTI)

    Yonezawa, Takayuki; Lee, Ji-Won; Hibino, Ayaka; Asai, Midori; Hojo, Hironori; Cha, Byung-Yoon; Teruya, Toshiaki; Nagai, Kazuo; Chung, Ung-Il; Yagasaki, Kazumi; and others

    2011-06-03

    Highlights: {yields} Harmine promotes the activity and mRNA expression of ALP. {yields} Harmine enhances the expressions of osteocalcin mRNA and protein. {yields} Harmine induces osteoblastic mineralization. {yields} Harmine upregulates the mRNA expressions of BMPs, Runx2 and Osterix. {yields} BMP signaling pathways are involved in the actions of harmine. -- Abstract: Bone mass is regulated by osteoblast-mediated bone formation and osteoclast-mediated bone resorption. We previously reported that harmine, a {beta}-carboline alkaloid, inhibits osteoclast differentiation and bone resorption in vitro and in vivo. In this study, we investigated the effects of harmine on osteoblast proliferation, differentiation and mineralization. Harmine promoted alkaline phosphatase (ALP) activity in MC3T3-E1 cells without affecting their proliferation. Harmine also increased the mRNA expressions of the osteoblast marker genes ALP and Osteocalcin. Furthermore, the mineralization of MC3T3-E1 cells was enhanced by treatment with harmine. Harmine also induced osteoblast differentiation in primary calvarial osteoblasts and mesenchymal stem cell line C3H10T1/2 cells. Structure-activity relationship studies using harmine-related {beta}-carboline alkaloids revealed that the C3-C4 double bond and 7-hydroxy or 7-methoxy group of harmine were important for its osteogenic activity. The bone morphogenetic protein (BMP) antagonist noggin and its receptor kinase inhibitors dorsomorphin and LDN-193189 attenuated harmine-promoted ALP activity. In addition, harmine increased the mRNA expressions of Bmp-2, Bmp-4, Bmp-6, Bmp-7 and its target gene Id1. Harmine also enhanced the mRNA expressions of Runx2 and Osterix, which are key transcription factors in osteoblast differentiation. Furthermore, BMP-responsive and Runx2-responsive reporters were activated by harmine treatment. Taken together, these results indicate that harmine enhances osteoblast differentiation probably by inducing the expressions of

  8. 1 Outreach, Education and Domestic Market Enhancement 2 Export Promotion and Assistance

    SciTech Connect (OSTI)

    Geothermal Energy Association

    2004-03-15

    Geothermal Energy Association supports the US geothermal industry in its efforts to bring more clean geothermal energy on-line throughout the world. Activities designed to accomplish this goal include: (1) developing and maintaining data bases, web pages, (2) commissioning of special studies and reports, (3) preparing, printing and distributing brochures and newsletters, (4) developing exhibits and displays, and participating in trade shows, (5) designing, producing and disseminating audio-video materials, (6) monitoring and coordinating programs carried out by US DOE and other Federal agencies, (7) holding workshops to facilitate communication between researchers and industry and to encourage their recognition of emerging markets for geothermal technology, (8) attending conferences, making speeches and presentation, and otherwise interacting with environmental and other renewable energy organizations and coalitions, (9) hosting events in Washington, DC and other appropriate locations to educate Federal, State and local representatives, environmental groups, the news media, and other about the status and potential of geothermal energy, (10) conducting member services such as the preparation and distribution of a member newsletter related to operating and maintaining s useful and viable association, and (11) performing similar kinds of activities designed to inform others about geothermal energy. The activities of the export promotion aim to assist industry in accomplishing the goal of successfully penetrating and developing energy in country with existing geothermal resources and a desire to develop them. Activities including in export promotion are: (1)needs analysis and assessment involve monitoring the progress of developing markets and projects overseas and working with US industry to determine what future activities by GEA would be of greatest assistance, (2) outreach includes the preparation and dissemination of brochures and videos for foreign professionals

  9. Promoting Behavior-Based Energy Efficiency in Military Housing

    SciTech Connect (OSTI)

    AH McMakin; EL Malone; RE Lundgren

    1999-09-07

    The U.S. Department of Energy's Federal Energy Management Program (FEMP) helps agencies reduce the cost of doing business through energy efficiency, water conservation, and the use of solar and other renewable energy. As a large energy user, the U.S. military has been one of the government sectors of focus. Several military installations have shown substantial energy savings in past years. Most of these efficiency projects, however, have focused primarily on physical upgrades, technologies, and purchasing habits. Furthermost projects have focused on administrative and operational areas of energy use. Military residential housing, in particular, has received little formal attention for energy efficiency involving behaviors of the residents themselves. Behavior-based change is a challenging, but potentially fruitful area for energy conservation programs. However, behavioral change involves links with values, social networks and organizations, and new ways of thinking about living patterns. This handbook attempts to fill a gap by offering guidance for promoting such efforts.

  10. Selection against spurious promoter motifs correlates withtranslational efficiency across bacteria

    SciTech Connect (OSTI)

    Froula, Jeffrey L.; Francino, M. Pilar

    2007-05-01

    Because binding of RNAP to misplaced sites could compromise the efficiency of transcription, natural selection for the optimization of gene expression should regulate the distribution of DNA motifs capable of RNAP-binding across the genome. Here we analyze the distribution of the -10 promoter motifs that bind the {sigma}{sup 70} subunit of RNAP in 42 bacterial genomes. We show that selection on these motifs operates across the genome, maintaining an over-representation of -10 motifs in regulatory sequences while eliminating them from the nonfunctional and, in most cases, from the protein coding regions. In some genomes, however, -10 sites are over-represented in the coding sequences; these sites could induce pauses effecting regulatory roles throughout the length of a transcriptional unit. For nonfunctional sequences, the extent of motif under-representation varies across genomes in a manner that broadly correlates with the number of tRNA genes, a good indicator of translational speed and growth rate. This suggests that minimizing the time invested in gene transcription is an important selective pressure against spurious binding. However, selection against spurious binding is detectable in the reduced genomes of host-restricted bacteria that grow at slow rates, indicating that components of efficiency other than speed may also be important. Minimizing the number of RNAP molecules per cell required for transcription, and the corresponding energetic expense, may be most relevant in slow growers. These results indicate that genome-level properties affecting the efficiency of transcription and translation can respond in an integrated manner to optimize gene expression. The detection of selection against promoter motifs in nonfunctional regions also implies that no sequence may evolve free of selective constraints, at least in the relatively small and unstructured genomes of bacteria.

  11. Chinese Renewable Energy Industries Association CREIA | Open...

    Open Energy Info (EERE)

    Product: CREIA promotes the adoption of advanced technologies among renewable energy enterprises in China and actively develops capacity for the rapid industrialisation of the...

  12. Principal Associate Director - Capital Projects

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    As Principal Associate Director for Capital Projects (interim), Larry Simmons is responsible for institutional large-project construction and management and environmental cleanup ...

  13. Principal Associate Director - Weapons Programs

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Weapons Programs As Principal Associate Director for the Weapons Program, Robert Webster leads the programs to assure the safety, security, and effectiveness of the systems in the...

  14. Weapons Program Associate Directors named

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    integration we have achieved between the various components of the program," said Bret Knapp, Principal Associate Director for Weapons Programs. "They have both done an...

  15. MicroRNA-196b promotes cell proliferation and suppress cell differentiation in vitro

    SciTech Connect (OSTI)

    Cao, Donglin Hu, Liangshan; Lei, Da; Fang, Xiaolin; Zhang, Zhihong; Wang, Ting; Lin, Maorui; Huang, Jiwei; Yang, Huawen; Zhou, Xuan; Zhong, Limei

    2015-01-30

    Highlights: • miRNA-196b increases proliferation and blocks differentiation of progenitor cell. • miRNA-196b inhibits apoptosis and increases viability of cells lines. • Forced expression of miR-196b blocks the differentiation of THP1 induced by PMA. - Abstract: MicroRNA-196b (miR-196b) is frequently amplified and aberrantly overexpressed in acute leukemias. To investigate the role of miR-196b in acute leukemias, it has been observed that forced expression of this miRNA increases proliferation and inhibits apoptosis in human cell lines. More importantly, we show that this miRNA can significantly increase the colony-forming capacity of mouse normal bone marrow progenitor cells alone, as well as partially blocking the cells from differentiation. Taken together, our studies suggest that miRNA-196b may play an essential role in the development of MLL-associated leukemias through inhibiting cell differentiation and apoptosis, while promoting cell proliferation.

  16. Stanniocalcin-2 is a HIF-1 target gene that promotes cell proliferation in hypoxia

    SciTech Connect (OSTI)

    Law, Alice Y.S.; Wong, Chris K.C.

    2010-02-01

    Stanniocalcin-2 (STC2), the paralog of STC1, has been suggested as a novel target of oxidative stress response to protect cells from apoptosis. The expression of STC2 has been reported to be highly correlated with human cancer development. In this study, we reported that STC2 is a HIF-1 target gene and is involved in the regulation of cell proliferation. STC2 was shown to be up-regulated in different breast and ovarian cancer cells, following exposure to hypoxia. Using ovarian cancer cells (SKOV3), the underlying mechanism of HIF-1 mediated STC2 gene transactivation was characterized. Hypoxia-induced STC2 expression was found to be HIF-1{alpha} dependent and required the recruitment of p300 and HDAC7. Using STC2 promoter deletion constructs and site-directed mutagenesis, two authentic consensus HIF-1 binding sites were identified. Under hypoxic condition, the silencing of STC2 reduced while the overexpression of STC2 increased the levels of phosphorylated retinoblastoma and cyclin D in both SKOV3 and MCF7 cells. The change in cell cycle proteins correlated with the data of the serial cell counts. The results indicated that cell proliferation was reduced in STC2-silenced cells but was increased in STC2-overexpressing hypoxic cells. Solid tumor progression is usually associated with hypoxia. The identification and functional analysis of STC2 up-regulation by hypoxia, a feature of the tumor microenvironment, sheds light on a possible role for STC2 in tumors.

  17. Promoting Uranium Immobilization by the Activities of Microbial Phosphatases

    SciTech Connect (OSTI)

    Robert J. Martinez; Melanie J. Beazley; Samuel M. Webb; Martial Taillefert; and Patricia A. Sobecky

    2007-04-19

    The overall objective of this project is to examine the activity of nonspecific phosphohydrolases present in naturally occurring subsurface microorganisms for the purpose of promoting the immobilization of radionuclides through the production of uranium [U(VI)] phosphate precipitates. Specifically, we hypothesize that the precipitation of U(VI) phosphate minerals may be promoted through the microbial release and/or accumulation of PO4 3- as a means to detoxify radionuclides and heavy metals. An experimental approach was designed to determine the extent of phosphatase activity in bacteria previously isolated from contaminated subsurface soils collected at the ERSP Field Research Center (FRC) in Oak Ridge, TN. Screening of 135 metal resistant isolates for phosphatase activity indicated the majority (75 of 135) exhibited a phosphatase-positive phenotype. During this phase of the project, a PCR based approach has also been designed to assay FRC isolates for the presence of one or more classes of the characterized non-specific acid phophastase (NSAP) genes likely to be involved in promoting U(VI) precipitation. Testing of a subset of Pb resistant (Pbr) Arthrobacter, Bacillus and Rahnella strains indicated 4 of the 9 Pbr isolates exhibited phosphatase phenotypes suggestive of the ability to bioprecipitate U(VI). Two FRC strains, a Rahnella sp. strain Y9602 and a Bacillus sp. strain Y9-2, were further characterized. The Rahnella sp. exhibited enhanced phosphatase activity relative to the Bacillus sp. Whole-cell enzyme assays identified a pH optimum of 5.5, and inorganic phosphate accumulated in pH 5.5 synthetic groundwater (designed to mimic FRC conditions) incubations of both strains in the presence of a model organophosphorus substrate provided as the sole C and P source. Kinetic experiments showed that these two organisms can grow in the presence of 200 μM dissolved uranium and that Rahnella is much more efficient in precipitating U(VI) than Bacillus sp. The

  18. Chemical inducible promoter used to obtain transgenic plants with a silent marker

    DOE Patents [OSTI]

    Aoyama, Takashi; Zuo, Jianru; Chua, Nam-Hai

    2004-08-31

    A chemically inducible promoter is described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoter described is one that is inducible by a glucocorticoid which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt or knotted1 to induce shoot formation in the presence of a glucocorticoid. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.

  19. Secretary Chu Announces New Efforts to Promote Clean Energy in Tribal

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Communities | Department of Energy Efforts to Promote Clean Energy in Tribal Communities Secretary Chu Announces New Efforts to Promote Clean Energy in Tribal Communities January 19, 2011 - 12:00am Addthis WASHINGTON - U.S. Energy Secretary Steven Chu announced today two new initiatives to promote tribal energy development and continue strengthening the partnership between the Department of Energy and tribal nations. Up to $10 million will be available this year through DOE's Tribal Energy

  20. Energy Secretary Bodman Heads to West Virginia to Promote Energy Bill |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Heads to West Virginia to Promote Energy Bill Energy Secretary Bodman Heads to West Virginia to Promote Energy Bill July 7, 2005 - 2:00pm Addthis Secretary Promotes Energizing America for Energy Security BELLE, WV - Secretary of Energy Samuel W. Bodman today traveled to West Virginia to urge the Congress to pass comprehensive energy legislation that is now before them. The bill reflects many of the principles of President Bush's national energy policy including the

  1. DOE Secretary Promotes E85 Use in Indianapolis | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Secretary Promotes E85 Use in Indianapolis DOE Secretary Promotes E85 Use in Indianapolis May 11, 2006 - 10:40am Addthis INDIANAPOLIS , IN - Department of Energy Secretary Samuel W. Bodman today praised and promoted the expanded use and availability of ethanol-based fuel during two events in Indianapolis. Expanding the use of ethanol, specifically cellulosic ethanol, is a key component of President Bush's Advanced Energy Initiative (AEI), which seeks to reduce our reliance on foreign oil by 5

  2. Executive Order 13583, Establishing a Coordinated Government-Wide Initiative to Promote Diversity and Inclusion in the Federal Workforce

    Broader source: Energy.gov [DOE]

    Establishes a "coordinated government-wide initiative to promote diversity and inclusion in the federal workforce".

  3. Processes and palladium-promoted catalysts for conducting Fischer-Tropsch synthesis

    DOE Patents [OSTI]

    Singleton, Alan H.; Oukaci, Rachid; Goodwin, James G.

    2000-01-01

    A process for hydrocarbon synthesis comprising the step of reacting a synthesis gas in the presence of a cobalt catalyst promoted with palladium.

  4. Energy Portfolio Standards and the Promotion of Combined Heat and Power (CHP) White Paper, April 2009

    Office of Energy Efficiency and Renewable Energy (EERE)

    EPA CHP Partnership’s white paper provides information on energy portfolio standards and how they promote combined heat and power.

  5. A New Scheme for the Promotion of Renewable Energies in Developing...

    Open Energy Info (EERE)

    Regulated Purchase Tariff Jump to: navigation, search Tool Summary LAUNCH TOOL Name: A New Scheme for the Promotion of Renewable Energies in Developing Countries: The Renewable...

  6. PEGylation of a Maltose Biosensor Promotes Enhanced Signal Response

    SciTech Connect (OSTI)

    Dattelbaum, Andrew; Baker, Gary A; Fox, John M; Iyer, Srinivas; Dattelbaum, Jonathan

    2009-01-01

    A robust method to immobilize a maltose biosensor is described using an engineered maltose periplasmic binding protein (PBP) covalently coupled to NBDamide, an environmentally sensitive fluorophore. A mesoporous silica sol-gel derived from diglycerylsilane (DGS) was constructed to embed the maltose biosensor, and the ligand reporting fluorescence properties were meas red. When sequestered in the DGS-derived silica matrix, the biosensor retained maltose-dependent fluorescence sensing capability with micromolar affinity, which is consistent with the protein free in solution. The MBP-NBD conjugate was further modified by covalent conjugation with poly(ethylene glycol)-5000 (PEG) to promote the retention of water molecules around the protein and to reduce possible steric effects between the silica matrix and protein. Bioconjugation with PEG molecules does not significantly affect the signaling response of the protein in solution. When immobilized in the DGS polymer, a consistent increase in fluorescence intensity was observed as compared to the protein not functionalized with PEG. To our knowledge, this report presents the first successful method to embed a PBP biosensor in a polymerized matrix and retain signaling response using an environmentally sensitive probe. The immobilization method presented here should be easily adaptable to all conformation-dependent biosensors.

  7. The Los Alamos universe: Using multimedia to promote laboratory capabilities

    SciTech Connect (OSTI)

    Kindel, J.

    2000-03-01

    This project consists of a multimedia presentation that explains the technological capabilities of Los Alamos National Laboratory. It takes the form of a human-computer interface built around the metaphor of the universe. The project is intended promote Laboratory capabilities to a wide audience. Multimedia is simply a means of communicating information through a diverse set of tools--be they text, sound, animation, video, etc. Likewise, Los Alamos National Laboratory is a collection of diverse technologies, projects, and people. Given the ample material available at the Laboratory, there are tangible benefits to be gained by communicating across media. This paper consists of three parts. The first section provides some basic information about the Laboratory, its mission, and its needs. The second section introduces this multimedia presentation and the metaphor it is based on along with some basic concepts of color and user interaction used in the building of this project. The final section covers construction of the project, pitfalls, and future improvements.

  8. International Hydropower Association | Open Energy Information

    Open Energy Info (EERE)

    International Hydropower Association Place: United Kingdom Zip: SM1 4JH Sector: Hydro Product: The International Hydropower Association is a non-governmental mutual association of...

  9. British Hydropower Association | Open Energy Information

    Open Energy Info (EERE)

    British Hydropower Association Place: Wimborne, Dorset, United Kingdom Zip: BH21 1QU Sector: Hydro Product: The British Hydropower Association (BHA) is a trade association which...

  10. World Wind Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Association Jump to: navigation, search Logo: World Wind Energy Association Name: World Wind Energy Association Address: Charles-de-Gaulle-Str. 5 Place: Bonn, Germany Phone Number:...

  11. European Wind Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Energy Association Jump to: navigation, search Logo: European Wind Energy Association Name: European Wind Energy Association Address: Rue d'Arlon 80 B-1040 Place: Brussels, Belgium...

  12. Research Affiliate Associates & Students | Photosynthetic Antenna...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Affiliate Associates & Students Research Affiliate Associates & Students Michal Gwizdala Postdoctoral Research Associate E-mail: m.s.gwizdala@vu.nl Atsuko Kanazawa Research...

  13. Kodiak Electric Association KEA | Open Energy Information

    Open Energy Info (EERE)

    Electric Association KEA Jump to: navigation, search Name: Kodiak Electric Association (KEA) Place: Kodiak, Alaska Zip: 99614 Product: Kodiak Electric Association, Inc. (KEA) is a...

  14. Electric Drive Transportation Association EDTA | Open Energy...

    Open Energy Info (EERE)

    Transportation Association EDTA Jump to: navigation, search Name: Electric Drive Transportation Association (EDTA) Product: EDTA is the preeminent U.S. industry association...

  15. Geothermal Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Association (Redirected from GEA) Jump to: navigation, search Logo: Geothermal Energy Association Name: Geothermal Energy Association Address: 209 Pennsylvania Avenue SE Place:...

  16. Geothermal Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Association Jump to: navigation, search Logo: Geothermal Energy Association Name: Geothermal Energy Association Address: 209 Pennsylvania Avenue SE Place: Washington, DC Zip: 20003...

  17. Northeast Sustainable Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Association Jump to: navigation, search Logo: Northeast Sustainable Energy Association Name: Northeast Sustainable Energy Association Address: 50 Miles Street Place: Greenfield,...

  18. German Biogas Association | Open Energy Information

    Open Energy Info (EERE)

    Association Jump to: navigation, search Name: German Biogas Association Place: Freising, Germany Zip: 85356 Product: The Association represents the interests of biogas plant...

  19. Association

    Office of Scientific and Technical Information (OSTI)

    ... is well known to exhibit16 I i quite intersting phenomena such as hysteresis and chaos. ... Lett. 23, 142 (1973). 8 H. Ikezi, J. S. DeGrassie and J. Drake, "Soliton generation at1...

  20. Association

    National Nuclear Security Administration (NNSA)

    ......... 7 ' 3.0 Accident Rates ......13 4. Frequencies of Accident Types (TIFA 1980-1989, tractor ...

  1. Association

    National Nuclear Security Administration (NNSA)

    This report describes the development of accident rate influence factors as related to the ......... 7 ' 3.0 Accident Rates ......

  2. Valley Electric Association- Net Metering

    Broader source: Energy.gov [DOE]

    The Board of Directors for Valley Electric Association (VEA) approved net metering in April 2008. The rules apply to systems up to 30 kW, though owners of larger systems may be able to negotiate...

  3. Principal Associate Director - Global Security

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Principal Associate Director - Global Security As Principal Associate Director for Global Security, Terry Wallace leads Laboratory programs with special focus on developing and applying the scientific and engineering capabilities to address complex national and global security threats. Contact Operator Los Alamos National Laboratory (505) 667-5061 Wallace's expertise is forensic seismology, a highly specialized discipline focusing on detection and quantification of nuclear tests. Terry C.

  4. Promoting system-level learning from project-level lessons

    SciTech Connect (OSTI)

    Jong, Amos A. de; Runhaar, Hens A.C.; Runhaar, Piety R.; Kolhoff, Arend J.; Driessen, Peter P.J.

    2012-02-15

    A growing number of low and middle income nations (LMCs) have adopted some sort of system for environmental impact assessment (EIA). However, generally many of these EIA systems are characterised by a low performance in terms of timely information dissemination, monitoring and enforcement after licencing. Donor actors (such as the World Bank) have attempted to contribute to a higher performance of EIA systems in LMCs by intervening at two levels: the project level (e.g. by providing scoping advice or EIS quality review) and the system level (e.g. by advising on EIA legislation or by capacity building). The aims of these interventions are environmental protection in concrete cases and enforcing the institutionalisation of environmental protection, respectively. Learning by actors involved is an important condition for realising these aims. A relatively underexplored form of learning concerns learning at EIA system-level via project level donor interventions. This 'indirect' learning potentially results in system changes that better fit the specific context(s) and hence contribute to higher performances. Our exploratory research in Ghana and the Maldives shows that thus far, 'indirect' learning only occurs incidentally and that donors play a modest role in promoting it. Barriers to indirect learning are related to the institutional context rather than to individual characteristics. Moreover, 'indirect' learning seems to flourish best in large projects where donors achieved a position of influence that they can use to evoke reflection upon system malfunctions. In order to enhance learning at all levels donors should thereby present the outcomes of the intervention elaborately (i.e. discuss the outcomes with a large audience), include practical suggestions about post-EIS activities such as monitoring procedures and enforcement options and stimulate the use of their advisory reports to generate organisational memory and ensure a better information dissemination.

  5. Low-temperature superacid catalysis: Reactions of n-butane catalyzed by iron- and manganese-promoted sulfated zirconia

    SciTech Connect (OSTI)

    Cheung, T.K.; D`Itri, J.L.; Gates, B.C.

    1995-02-01

    Environmental concerns are leading to the replacement of aromatic hydrocarbons in gasoline with high-octane-number branched paraffins and oxygenated compounds such as methyl t-butyl ether, which is produced from methanol and isobutylene. The latter can be formed from n-butane by isomerization followed by dehydrogenation. To meet the need for improved catalysts for isomerization of n-butane and other paraffins, researchers identified solid acids that are noncorrosive and active at low temperatures. Sulfated zirconia catalyzes the isomerization of n-butane even at 25{degrees}C, and the addition of Fe and Mn promoters increases its activity by three orders of magnitude. Little is known about this new catalyst. Here the authors provide evidence of its performance for n-butane conversion, demonstrating that isomerization is accompanied by disproportionation and other, less well understood, acid-catalyzed reactions and undergoes rapid deactivation associated with deposition of carbonaceous material. 10 refs., 3 figs.

  6. Postdoctoral Associates | Photosynthetic Antenna Research Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Postdoctoral Associates Postdoctoral Associates Meghan Barnhart-Dailey Postdoctoral Research Associate Read more about Meghan Barnhart-Dailey Reddy Muthyala Nagarjuna Postdoctoral Research Associate Read more about Reddy Muthyala Nagarjuna David Swainsbury Postdoctoral Research Associate Read more about David Swainsbury Amit Mandal Postdoctoral Research Associate Read more about Amit Mandal Xun Lu Postdoctoral Research Associate Read more about Xun Lu Durgesh Rai Postdoctoral Research Associate

  7. Environmental Management Associates | Open Energy Information

    Open Energy Info (EERE)

    Environmental Management Associates Jump to: navigation, search Name: Environmental Management Associates Abbreviation: EMA Website: www.emacorp.com Environmental Management...

  8. Conventional Energy Forum & Associated Vertical Business Development...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Conventional Energy Forum & Associated Vertical Business Development: Best Practices in Indian Country Conventional Energy Forum & Associated Vertical Business Development: Best ...

  9. NNSA Procurement Projects Perspective - Bob Raines, Associate...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Projects Perspective - Bob Raines, Associate Administrator for Acquisition and Project Management, NNSA NNSA Procurement Projects Perspective - Bob Raines, Associate Administrator ...

  10. Voluntary Protection Programs Participants' Association (VPPPA...

    Office of Environmental Management (EM)

    Programs Participants' Association (VPPPA) Presentation: Conducting your Annual VPP Self Assessment 2009 Voluntary Protection Programs Participants' Association (VPPPA)...

  11. 2009 Voluntary Protection Programs Participants' Association...

    Office of Environmental Management (EM)

    Programs Participants' Association (VPPPA) Presentation: Conducting your Annual VPP Self Assessment 2009 Voluntary Protection Programs Participants' Association (VPPPA)...

  12. VDE Association for Electrical Electronic Information Technologies...

    Open Energy Info (EERE)

    VDE Association for Electrical Electronic Information Technologies Jump to: navigation, search Name: VDE (Association for Electrical, Electronic & Information Technologies) Place:...

  13. Voluntary Protection programs Participants' Association - Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Protection Programs Participants' Association (VPPPA) Presentations: Star Track Voluntary Protection Programs Participants' Association (VPPPA) Presentation: Wellness Programs

  14. Ethanol Steam Reforming on Co/CeO2: The Effect of ZnO Promoter...

    Office of Scientific and Technical Information (OSTI)

    Ethanol Steam Reforming on CoCeO2: The Effect of ZnO Promoter Citation Details In-Document Search Title: Ethanol Steam Reforming on CoCeO2: The Effect of ZnO Promoter A series of ...

  15. Orbital fluid shear stress promotes osteoblast metabolism, proliferation and alkaline phosphates activity in vitro

    SciTech Connect (OSTI)

    Aisha, M.D.; Nor-Ashikin, M.N.K.; Sharaniza, A.B.R.; Nawawi, H.; Froemming, G.R.A.

    2015-09-10

    Prolonged disuse of the musculoskeletal system is associated with reduced mechanical loading and lack of anabolic stimulus. As a form of mechanical signal, the multidirectional orbital fluid shear stress transmits anabolic signal to bone forming cells in promoting cell differentiation, metabolism and proliferation. Signals are channeled through the cytoskeleton framework, directly modifying gene and protein expression. For that reason, we aimed to study the organization of Normal Human Osteoblast (NHOst) cytoskeleton with regards to orbital fluid shear (OFS) stress. Of special interest were the consequences of cytoskeletal reorganization on NHOst metabolism, proliferation, and osteogenic functional markers. Cells stimulated at 250 RPM in a shaking incubator resulted in the rearrangement of actin and tubulin fibers after 72 h. Orbital shear stress increased NHOst mitochondrial metabolism and proliferation, simultaneously preventing apoptosis. The ratio of RANKL/OPG was reduced, suggesting that orbital shear stress has the potential to inhibit osteoclastogenesis and osteoclast activity. Increase in ALP activity and OCN protein production suggests that stimulation retained osteoblast function. Shear stress possibly generated through actin seemed to hold an anabolic response as osteoblast metabolism and functional markers were enhanced. We hypothesize that by applying orbital shear stress with suitable magnitude and duration as a non-drug anabolic treatment can help improve bone regeneration in prolonged disuse cases. - Highlights: • OFS stress transmits anabolic signals to osteoblasts. • Actin and tubulin fibers are rearranged under OFS stress. • OFS stress increases mitochondrial metabolism and proliferation. • Reduced RANKL/OPG ratio in response to OFS inhibits osteoclastogenesis. • OFS stress prevents apoptosis and stimulates ALP and OCN.

  16. Washington State Energy-Efficiency Appliance Rebate Program (SEEARP) Spurs Local Economy, Promotes State-Wide Partnership

    Broader source: Energy.gov [DOE]

    State of Washington works to save money, spur economic activity, and promote energy-efficient appliance options.

  17. Methods for promoting wound healing and muscle regeneration with the cell signaling protein Nell1

    DOE Patents [OSTI]

    Culiat, Cymbeline T.

    2011-03-22

    The present invention provides methods for promoting wound healing and treating muscle atrophy in a mammal in need. The method comprises administering to the mammal a Nell1 protein or a Nell1 nucleic acid molecule.

  18. Role of copper promotion in precipitated iron Fischer-Tropsch catalysts

    SciTech Connect (OSTI)

    O`Brien, R.J.; Xu, L.; Davis, B.H.

    1996-10-01

    Slurry phase Fischer-Tropsch synthesis was conducted on precipitated iron-silicon catalysts. The affect of copper promotion on the activity and selectivity of carbon monoxide, hydrogen and syngas activated catalysts is presented. High activity and stability have been obtained for potassium promoted catalysts when operating at 270{degrees}C; however, it has been found that promotion with potassium and copper is essential to obtaining good activity in a wax producing mode at 230{degrees}C. Promotion with copper is critical to achieving good activity when pretreating catalysts with hydrogen or with syngas at high pressure. XRD and Mossbauer data indicate that copper facilitates the reduction of iron oxide to metallic iron and iron carbides during hydrogen and syngas pretreatments.

  19. Toward a comprehensive understanding of rideshare promotion: The use of evaluation research

    SciTech Connect (OSTI)

    Horan, T.A.

    1988-01-01

    With traffic congestion becoming a serious problem in many metropolitan areas, rideshare promotion is increasingly being viewed as an important congestion reduction strategy. Since the oil embargo of 1973-74, the primary governmental mechanism for promoting ridesharing has been through rideshare agencies (RSAs). These RSAs work mainly with local employers to promote ridesharing to employees. Recent evidence suggests that RSAs could be more effective if they worked to develop comprehensive employer rideshare programs, rather than focusing predominately on the provision of matchlists to employees. This study, drawing upon the theory-driven approach suggested by Chen and Rossi (1983), delineated a program model for a more comprehensive rideshare promotional effort, and then executed an evaluation based upon several hypothesized relationship. Results confirmed several of the hypothesized relationships. In particular, rideshare program implementation had a significant bivariate relationship with employee rideshare placement. Several expected (and unexpected) employer and employee variables also had significant relationships with rideshare placement.

  20. Hong Qin promoted to executive dean at the University of Science...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hong Qin promoted to executive dean at the University of Science and Technology of China ... University of Science and Technology of China (USTC), which named him executive dean of ...

  1. DOE Project Leads to New Alliance to Promote Low-Impact Drilling

    Broader source: Energy.gov [DOE]

    A project supported by the Office of Fossil Energy's National Energy Technology Laboratory (NETL) has given rise to a major new research consortium to promote advanced technology for low-impact oil and gas drilling.

  2. Methods for promoting wound healing and muscle regeneration with the cell signaling protein Nell1

    SciTech Connect (OSTI)

    Culiat, Cymbeline T

    2014-11-04

    The present invention provides methods for promoting wound healing and treating muscle atrophy in a mammal in need. The method comprises administering to the mammal a Nell1 protein or a Nell1 nucleic acid molecule.

  3. Department of Energy and Disney Join Forces to Promote Energy Savings

    Broader source: Energy.gov [DOE]

    WASHINGTON, DC - U.S. Department of Energy (DOE) Secretary Samuel W. Bodman today announced that DOE and The Walt Disney Company are cooperating in a nationwide campaign to promote energy...

  4. Pseudogene CYP4Z2P 3'UTR promotes angiogenesis in breast cancer...

    Office of Scientific and Technical Information (OSTI)

    Title: Pseudogene CYP4Z2P 3'UTR promotes angiogenesis in breast cancer Highlights: * A new ... were studied. * The mechanism provides new insights for the breast cancer progression. ...

  5. Will Dichtel > Associate Professor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Chemistry and Chemical Biology > Faculty Directory > The Energy Materials Center at Cornell Will Dichtel Associate Professor Chemistry and Chemical Biology Research Group Webpage wrd33@cornell.edu The Dichtel Group uses the tools of synthetic and supramolecular chemistry to develop structurally precise organic materials. Projects in the Dichtel Group involve organic and polymer chemistry, solution and solid-state characterization, nanofabrication, and electronic testing of materials.

  6. Bradbury Science Museum Association Volunteer

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Volunteers Volunteers Want to get more involved with the museum? We have several opportunities to either become a volunteer or request one at your event. Contact Us thumbnail of Bradbury Science Museum (505) 667-4444 Email Bradbury Science Museum Association Volunteer This official non-profit partner of the Bradbury Science Museum provides many opportunities to be more involved with the Bradbury's programs through work such as researching collections items and giving docent tours of the Bradbury

  7. Associated Post Office Box 117

    Office of Legacy Management (LM)

    Associated Post Office Box 117 Oak Ridge Tennessee 37831 -01 17 Energy Enuronmerl! Systems DIWSIO~I September 7, 1990 ? -iAsWP / Fll ,- ,) 3 Mr. Alexander Williams Office of Environmental Restoration and Waste Management U.S. Department of Energy Washington, DC 20545 Subject: PRELIMINARY SITE VISIT - REVERE COPPER AND BRASS CORP. Dear Mr. Williams: This letter is in response to your request concerning the results of the preliminary site visit to the former Revere Copper and Brass Corporation

  8. Secretary Chu Announces Initiatives to Promote Clean Energy at First Clean

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Ministerial | Department of Energy Initiatives to Promote Clean Energy at First Clean Energy Ministerial Secretary Chu Announces Initiatives to Promote Clean Energy at First Clean Energy Ministerial July 20, 2010 - 12:00am Addthis Washington, D.C. - At the world's first Clean Energy Ministerial, U.S. Energy Secretary Steven Chu today announced that the United States is helping launch more than 10 international clean energy initiatives. These initiatives will cut energy waste; help

  9. Energy Department Awards Will Promote Electric Vehicles in 24 States and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Train a Workforce for Advanced Vehicle Development | Department of Energy Will Promote Electric Vehicles in 24 States and Train a Workforce for Advanced Vehicle Development Energy Department Awards Will Promote Electric Vehicles in 24 States and Train a Workforce for Advanced Vehicle Development September 8, 2011 - 3:17pm Addthis Washington, D.C. - U.S. Energy Secretary Steven Chu today announced 16 projects supporting activities in 24 states and the District of Columbia to accelerate the

  10. Secretary Chu Announces New Efforts to Promote Clean Energy in Tribal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Communities Secretary Chu Announces New Efforts to Promote Clean Energy in Tribal Communities Secretary Steven Chu WASHINGTON � U.S. Energy Secretary Steven Chu announced today two new initiatives to promote tribal energy development and continue strengthening the partnership between the Department of Energy and tribal nations. Up to $10 million will be available this year through DOE�s Tribal Energy Program to support the evaluation, development and deployment of energy efficiency and