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Sample records for association rfa promotes

  1. Microsoft PowerPoint - RFA_EIA_conference_2009.ppt

    U.S. Energy Information Administration (EIA) Indexed Site

    America's Ethanol Industry Our Energy Future Starts at Home About the RFA * The Renewable Fuels Association (RFA) promotes policies, regulations and research and development initiatives that will lead to the increased production and use of fuel ethanol. * RFA membership includes a broad cross- section of businesses, individuals and organizations dedicated to the expansion of the U.S. fuel ethanol industry. Ethanol Facts 2008 Production / 2009 Estimate 9.2 billion gallons / 10.5 billion gallons

  2. RFA-14-0001 - In the Matter of Commonwealth Oil Refining Company...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    RFA-14-0001 - In the Matter of Commonwealth Oil Refining Company, Inc.Commonwealth of Puerto Rico RFA-14-0001 - In the Matter of Commonwealth Oil Refining Company, Inc....

  3. RCRA Facilities Assessment (RFA)---Oak Ridge National Laboratory

    SciTech Connect (OSTI)

    Not Available

    1987-03-01

    US Department of Energy (DOE) facilities are required to be in full compliance with all federal and state regulations. In response to this requirement, the Oak Ridge National Laboratory (ORNL) has established a Remedial Action Program (RAP) to provide comprehensive management of areas where past and current research, development, and waste management activities have resulted in residual contamination of facilities or the environment. This report presents the RCRA Facility Assessment (RFA) required to meet the requirements of RCRA Section 3004(u). Included in the RFA are (1) a listing of all sites identified at ORNL that could be considered sources of releases or potential releases; (2) background information on each of these sites, including location, type, size, period of operation, current operational status, and information on observed or potential releases (as required in Section II.A.1 of the RCRA permit); (3) analytical results obtained from preliminary surveys conducted to verify the presence or absence of releases from some of the sites; and (4) ORNL`s assessment of the need for further remedial attention.

  4. RCRA Facilities Assessment (RFA)---Oak Ridge National Laboratory

    SciTech Connect (OSTI)

    Not Available

    1987-03-01

    US Department of Energy (DOE) facilities are required to be in full compliance with all federal and state regulations. In response to this requirement, the Oak Ridge National Laboratory (ORNL) has established a Remedial Action Program (RAP) to provide comprehensive management of areas where past and current research, development, and waste management activities have resulted in residual contamination of facilities or the environment. This report presents the RCRA Facility Assessment (RFA) required to meet the requirements of RCRA Section 3004(u). Included in the RFA are (1) a listing of all sites identified at ORNL that could be considered sources of releases or potential releases; (2) background information on each of these sites, including location, type, size, period of operation, current operational status, and information on observed or potential releases (as required in Section II.A.1 of the RCRA permit); (3) analytical results obtained from preliminary surveys conducted to verify the presence or absence of releases from some of the sites; and (4) ORNL's assessment of the need for further remedial attention.

  5. ORISE: Iddins promoted to associate director of Radiation Emergency

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Assistance Center/Training Site Iddins promoted to associate director of Radiation Emergency Assistance Center/Training Site New director brings more than 20 years of medical expertise FOR IMMEDIATE RELEASE Oct. 14, 2014 FY15-01 Dr. Carol Iddins Dr. Carol Iddins (Click for high resolution version) OAK RIDGE, Tenn.-Dr. Carol J. Iddins has been named associate director of the Radiation Emergency Assistance Center/Training Site (REAC/TS). In this position, Iddins will provide management and

  6. RCRA Facilities Assessment (RFA) Oak Ridge National Laboratory addendum August 25, 1987

    SciTech Connect (OSTI)

    Not Available

    1987-08-01

    The RCRA Facilities Assessment (RFA) report identified approximately 250 Solid Waste Management Units (SWMUs) that were grouped into 20 Waste Area Groupings (WAGs) at Oak Ridge National Laboratory. Identification of each SWMU included information as to location, type, size, dates of operation, type of waste handled, and evidence of releases. Preliminary sampling studies were performed around each WAG to determine if there was evidence of releases beyond its perimeter. Analytical results from the surveys and historical information were the basis for recommendations concerning further actions for each WAG. Remedial investigations (RIs) were recommended for WAGs 1--10 and 17; for WAGs 14, 16, 18, and 20, it was suggested that they be removed from further consideration for remedial action. For the remaining WAGs (11, 12, 13, 15, and 19) the evidence concerning the possible release of contaminants was inconclusive and additional sampling was recommended. The purpose of this Addendum is to report the analytical data obtained from the additional surveys, to make recommendations concerning future remedial actions within these WAGs, and to provide descriptive information for additional sites listed in Table 1.2 of the RFA. Since information concerning the rationale for identifying releases, the sampling survey methodology, and background information for each WAG is presented in the RFA, it is not repeated in this Addendum.

  7. Image-Guided Radiofrequency Ablation (RFA) of Unresectable Hepatic Tumors Using a Triple-Spiral-Shaped Electrode Needle: Initial Experience in 34 Patients

    SciTech Connect (OSTI)

    Thanos, Loukas; Poulou, Loukia S.; Ziakas, Panayiotis D.; Kelekis, Alexis D.; Pomoni, Maria; Kelekis, Dimitrios A.

    2010-02-15

    We evaluated the safety and efficacy of image-guided radiofrequency ablation (RFA) using a triple-spiral-shaped electrode needle for unresectable primary or metastatic hepatic tumors. Thirty-four patients with 46 index tumors were treated. Ablation zone, morbidity, and complications were assessed. The lesions were completely ablated with an ablative margin of about 1 cm. Five patients (14.7%) with a lesion larger than 4.5 cm had local tumor progression after 1 month and were retreated. Hemothorax, as a major complication, occurred in 1 of 34 patients (3.0%) or 1 of 46 lesions ablated (2.2%). RFA using this new electrode needle can be effective in the treatment of large unresectable hepatic tumors.

  8. Transcriptional Responses to Sucrose Mimic the Plant-Associated Life Style of the Plant Growth Promoting Endophyte Enterobacter sp. 638

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Taghavi, Safiyh; Wu, Xiao; Ouyang, Liming; Zhang, Yian Biao; Stadler, Andrea; McCorkle, Sean; Zhu, Wei; Maslov, Sergei; van der Lelie, Daniel

    2015-01-21

    Growth in sucrose medium was previously found to trigger the expression of functions involved in the plant associated life style of the endophytic bacterium Enterobacter sp. 638. Therefore, comparative transcriptome analysis between cultures grown in sucrose or lactate medium was used to gain insights in the expression levels of bacterial functions involved in the endophytic life style of strain 638. Growth on sucrose as a carbon source resulted in major changes in cell physiology, including a shift from a planktonic life style to the formation of bacterial aggregates. This shift was accompanied by a decrease in transcription of genes involvedmore » in motility (e.g. flagella biosynthesis) and an increase in the transcription of genes involved in colonization, adhesion and biofilm formation. The transcription levels of functions previously suggested as being involved in endophytic behavior and functions responsible for plant growth promoting properties, including the synthesis of indole-acetic acid, acetoin and 2,3-butanediol, also increased significantly for cultures grown in sucrose medium. Interestingly, despite an abundance of essential nutrients transcription levels of functions related to uptake and processing of nitrogen and iron became increased for cultures grown on sucrose as sole carbon source. Transcriptome data were also used to analyze putative regulatory relationships. In addition to the small RNA csrABCD regulon, which seems to play a role in the physiological adaptation and possibly the shift between free-living and plant-associated endophytic life style of Enterobacter sp. 638, our results also pointed to the involvement of rcsAB in controlling responses by Enterobacter sp. 638 to a plant-associated life style. Lastly, targeted mutagenesis was used to confirm this role and showed that compared to wild-type Enterobacter sp. 638 a ΔrcsB mutant was affected in its plant growth promoting ability.« less

  9. Transcriptional Responses to Sucrose Mimic the Plant-Associated Life Style of the Plant Growth Promoting Endophyte Enterobacter sp. 638

    SciTech Connect (OSTI)

    Taghavi, Safiyh; Wu, Xiao; Ouyang, Liming; Zhang, Yian Biao; Stadler, Andrea; McCorkle, Sean; Zhu, Wei; Maslov, Sergei; van der Lelie, Daniel

    2015-01-21

    Growth in sucrose medium was previously found to trigger the expression of functions involved in the plant associated life style of the endophytic bacterium Enterobacter sp. 638. Therefore, comparative transcriptome analysis between cultures grown in sucrose or lactate medium was used to gain insights in the expression levels of bacterial functions involved in the endophytic life style of strain 638. Growth on sucrose as a carbon source resulted in major changes in cell physiology, including a shift from a planktonic life style to the formation of bacterial aggregates. This shift was accompanied by a decrease in transcription of genes involved in motility (e.g. flagella biosynthesis) and an increase in the transcription of genes involved in colonization, adhesion and biofilm formation. The transcription levels of functions previously suggested as being involved in endophytic behavior and functions responsible for plant growth promoting properties, including the synthesis of indole-acetic acid, acetoin and 2,3-butanediol, also increased significantly for cultures grown in sucrose medium. Interestingly, despite an abundance of essential nutrients transcription levels of functions related to uptake and processing of nitrogen and iron became increased for cultures grown on sucrose as sole carbon source. Transcriptome data were also used to analyze putative regulatory relationships. In addition to the small RNA csrABCD regulon, which seems to play a role in the physiological adaptation and possibly the shift between free-living and plant-associated endophytic life style of Enterobacter sp. 638, our results also pointed to the involvement of rcsAB in controlling responses by Enterobacter sp. 638 to a plant-associated life style. Lastly, targeted mutagenesis was used to confirm this role and showed that compared to wild-type Enterobacter sp. 638 a ΔrcsB mutant was affected in its plant growth promoting ability.

  10. Overexpressed KDM5B is associated with the progression of glioma and promotes glioma cell growth via downregulating p21

    SciTech Connect (OSTI)

    Dai, Bin; Hu, Zhiqiang; Huang, Hui; Zhu, Guangtong; Xiao, Zhiyong; Wan, Weiqing; Zhang, Peng; Jia, Wang; Zhang, Liwei

    2014-11-07

    Highlights: • KDM5B is overexpressed in glioma samples. • KDM5B stimulated proliferation of glioma cells. • Inhibition of p21contributes to KDM5B-induced proliferation. - Abstract: Epigenetic alterations such as aberrant expression of histone-modifying enzymes have been implicated in tumorigenesis. Upregulation of lysine (K)-specific demethylase 5B (KDM5B) has been reported in a variety of malignant tumors. However, the impact of KDM5B in glioma remains unclear. The objective of this study was to investigate the expression and prognostic value of KDM5B in glioma. In clinical glioma samples, we found that KDM5B expression was significantly upregulated in cancer lesions compared with normal brain tissues. Kaplan–Meier analysis showed that patients with glioma and higher KDM5B expression tend to have shorter overall survival time. By silencing or overexpressing KDM5B in glioma cells, we found that KDM5B could promote cell growth both in vitro and in vivo. Moreover, we demonstrated that KDM5B promoted glioma proliferation partly via regulation of the expression of p21. Our study provided evidence that KDM5B functions as a novel tumor oncogene in glioma and may be a potential therapeutic target for glioma management.

  11. CDK-associated Cullin 1 promotes cell proliferation with activation of ERK1/2 in human lung cancer A549 cells

    SciTech Connect (OSTI)

    Chen, Tian Jun; Gao, Fei; Yang, Tian; Thakur, Asmitanand; Ren, Hui; Li, Yang; Zhang, Shuo; Wang, Ting; Chen, Ming Wei

    2013-07-19

    Highlights: CDK-associated Cullin 1 (CAC1) expression increases in human lung carcinoma. CAC1 promotes the proliferation of lung cancer A549 cells. CAC1 promotes human lung cancer A549 cell proliferation with activation of ERK1/2. -- Abstract: Lung cancer is one of the most common causes of cancer-related death in the world, but the mechanisms remain unknown. In this study, we investigated the expression of CDK-associated Cullin 1 (CAC1) in lung cancer, the effect of CAC1 on the proliferation of human lung cancer A549 cells, and the activation of signaling pathways of mitogen-activated protein kinases (MAPKs). Results showed that CAC1 expression was higher levels in human lung carcinoma than normal lung tissue, and CAC1 siRNA reduced the proliferation of lung cancer A549 cells by decreasing cell activity and cell division in vitro. The proportion of cells treated with CAC1 siRNA increased in the G1 phase and decreased in the S and G2/M phase, indicative of G1 cell cycle arrest. Furthermore, the proportions of early/late apoptosis in lung cancer A549 cells were enhanced with CAC1 siRNA treatment. It was also found that activation of extracellular signal-regulated protein kinase (ERK) and p38 signaling pathways were involved in the proliferation of A549 cells. After CAC1 siRNA treatment, p-ERK1/2 levels decreased, and meanwhile p-p38 level increased, A549 cell proliferation increased when ERK1/2 signaling is activated by PMA. Our findings demonstrated that CAC1 promoted the proliferation of human lung cancer A549 cells with activation of ERK1/2 signaling pathways, suggesting a potential cure target for treatment of human lung cancer.

  12. Cancer cell-associated cytoplasmic B7–H4 is induced by hypoxia through hypoxia-inducible factor-1α and promotes cancer cell proliferation

    SciTech Connect (OSTI)

    Jeon, You-Kyoung; Park, Sae-Gwang; Choi, Il-Whan; Lee, Soo-Woong; Lee, Sang Min

    2015-04-03

    Aberrant B7–H4 expression in cancer tissues serves as a novel prognostic biomarker for poor survival in patients with cancer. However, the factor(s) that induce cancer cell-associated B7–H4 remain to be fully elucidated. We herein demonstrate that hypoxia upregulates B7–H4 transcription in primary CD138{sup +} multiple myeloma cells and cancer cell lines. In support of this finding, analysis of the Multiple Myeloma Genomics Portal (MMGP) data set revealed a positive correlation between the mRNA expression levels of B7–H4 and the endogenous hypoxia marker carbonic anhydrogenase 9. Hypoxia-induced B7–H4 expression was detected in the cytoplasm, but not in cancer cell membranes. Chromatin immunoprecipitation analysis demonstrated binding of hypoxia-inducible factor-1α (HIF-1α) to proximal hypoxia-response element (HRE) sites within the B7–H4 promoter. Knockdown of HIF-1α and pharmacological inhibition of HIF-1α diminished B7–H4 expression. Furthermore, knockdown of cytoplasmic B7–H4 in MCF-7 decreased the S-phase cell population under hypoxia. Finally, MMGP analysis revealed a positive correlation between the transcript levels of B7–H4 and proliferation-related genes including MKI67, CCNA1, and Myc in several patients with multiple myeloma. Our results provide insight into the mechanisms underlying B7–H4 upregulation and its role in cancer cell proliferation in a hypoxic tumor microenvironment. - Highlights: • Hypoxia upregulates B7–H4 transcription and protein expression. • Hypoxia-induced B7–H4 is detected in the cytoplasm, but not on membrane. • ChIP assay reveals a binding of HIF-1α to B7–H4 promoter at HRE site. • Knockdown and pharmacological inhibition of HIF-1α reduce B7–H4 expression. • B7–H4 knockdown decrease the number of cells in S-phase of cell cycle.

  13. Functional Promoter Variant rs2868371 of HSPB1 Is Associated With Risk of Radiation Pneumonitis After Chemoradiation for Non-Small Cell Lung Cancer

    SciTech Connect (OSTI)

    Pang, Qingsong; Department of Radiation Oncology and Lung Cancer Center, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy, Tianjin ; Wei, Qingyi; Xu, Ting; Yuan, Xianglin; Lopez Guerra, Jose Luis; Levy, Lawrence B.; Liu, Zhensheng; Gomez, Daniel R.; Zhuang, Yan; Wang, Li-E.; Mohan, Radhe; Komaki, Ritsuko; Liao, Zhongxing

    2013-04-01

    Purpose: To date, no biomarkers have been found to predict, before treatment, which patients will develop radiation pneumonitis (RP), a potentially fatal toxicity, after chemoradiation for lung cancer. We investigated potential associations between single nucleotide polymorphisms (SNPs) in HSPB1 and risk of RP after chemoradiation for non-small cell lung cancer (NSCLC). Methods and Materials: Subjects were patients with NSCLC treated with chemoradiation at 1 institution. The training data set comprised 146 patients treated from 1999 to July 2004; the validation data set was 125 patients treated from August 2004 to March 2010. We genotyped 2 functional SNPs of HSPB1 (rs2868370 and rs2868371) from all patients. We used Kaplan-Meier analysis to assess the risk of grade ≥2 or ≥3 RP in both data sets and a parametric log-logistic survival model to evaluate the association of HSPB1 genotypes with that risk. Results: Grade ≥3 RP was experienced by 13% of those with CG/GG and 29% of those with CC genotype of HSPB1 rs2868371 in the training data set (P=.028); corresponding rates in the validation data set were 2% CG/GG and 14% CC (P=.02). Univariate and multivariate analysis confirmed the association of CC of HSPB1 rs2868371 with higher risk of grade ≥3 RP than CG/GG after adjustment for sex, age, performance status, and lung mean dose. This association was validated both in the validation data set and with Harrell's C statistic. Conclusions: The CC genotype of HSPB1 rs2868371 was associated with severe RP after chemoradiation for NSCLC.

  14. Arsenic and chromium in drinking water promote tumorigenesis in a mouse colitis-associated colorectal cancer model and the potential mechanism is ROS-mediated Wnt/β-catenin signaling pathway

    SciTech Connect (OSTI)

    Wang, Xin; Mandal, Ardhendu K.; Saito, Hiroshi; Pulliam, Joseph F.; Lee, Eun Y.; Ke, Zun-Ji; Lu, Jian; Ding, Songze; Li, Li; Shelton, Brent J.; Tucker, Thomas; Evers, B. Mark; Zhang, Zhuo; Shi, Xianglin

    2012-07-01

    Exposure to carcinogenic metals, such as trivalent arsenic [As(III)] and hexavalent chromium [Cr(VI)], through drinking water is a major global public health problem and is associated with various cancers. However, the mechanism of their carcinogenicity remains unclear. In this study, we used azoxymethane/dextran sodium sulfate (AOM/DSS)-induced mouse colitis-associated colorectal cancer model to investigate their tumorigenesis. Our results demonstrate that exposure to As(III) or Cr(VI), alone or in combination, together with AOM/DSS pretreatment has a promotion effect, increasing the colorectal tumor incidence, multiplicity, size, and grade, as well as cell inflammatory response. Two-dimensional differential gel electrophoresis coupled with mass spectrometry revealed that As(III) or Cr(VI) treatment alone significantly changed the density of proteins. The expression of β-catenin and phospho-GSK was increased by treatment of carcinogenic metals alone. Concomitantly, the expression of NADPH oxidase1 (NOX1) and the level of 8-OHdG were also increased by treatment of carcinogenic metals alone. Antioxidant enzymes, such as superoxide dismutase (SOD) and catalase, were decreased. Similarly, in an in vitro system, exposure of CRL-1807 to carcinogenic metals increased reactive oxygen species (ROS) generation, the expression of β-catenin, phospho-GSK, and NOX1. Inhibition of ROS generation by addition of SOD or catalase inhibited β-catenin expression and activity. Our study provides a new animal model to study the carcinogenicity of As(III) and Cr(VI) and suggests that As(III) and Cr(VI) promote colorectal cancer tumorigenesis, at least partly, through ROS-mediated Wnt/β-catenin signaling pathway. -- Highlights: ► Carcinogenic metals in drinking water promote colorectal tumor formation in vivo. ► Carcinogenic metals induce β-catenin activation in vivo and in vitro. ► ROS generation induced by carcinogenic metals mediated β-catenin activation.

  15. Commercial Current Promotions

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New Commercial Program Development Commercial Current Promotions Industrial Federal Agriculture This page features all current special promotions for commercial programs....

  16. High expression Zymomonas promoters

    DOE Patents [OSTI]

    Viitanen, Paul V.; Tao, Luan; Zhang, Yuying; Caimi, Perry G.; McCole, Laura : Zhang, Min; Chou, Yat-Chen; McCutchen, Carol M.; Franden, Mary Ann

    2011-08-02

    Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.

  17. Environmental Markets Association | Open Energy Information

    Open Energy Info (EERE)

    20005 Product: The Environmental Markets Association (EMA), an international nonprofit trade association, was originally formed in 1997 to promote market-based trading solutions...

  18. Antonya Sanders-Promoting

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Antonya Sanders-Promoting nanoscience integration through outreach March 17, 2014 Promoting nanoscience integration through outreach Although raised in Los Alamos, Antonya Sanders knows what it is like to be a foreigner. The daughter of a Czech immigrant, Sanders lived in England and traveled the world to find ways to help others, including aiding Bosnian refugees. She lived on the Pacific island of Kiribati, where she was a Peace Corps' volunteer and teacher for three years, living in a thatch

  19. Genome-wide analysis of promoter architecture in Drosophila melanogaster

    SciTech Connect (OSTI)

    Hoskins, Roger A.; Landolin, Jane M.; Brown, James B.; Sandler, Jeremy E.; Takahashi, Hazuki; Lassmann, Timo; Yu, Charles; Booth, Benjamin W.; Zhang, Dayu; Wan, Kenneth H.; Yang, Li; Boley, Nathan; Andrews, Justen; Kaufman, Thomas C.; Graveley, Brenton R.; Bickel, Peter J.; Carninci, Piero; Carlson, Joseph W.; Celniker, Susan E.

    2010-10-20

    Core promoters are critical regions for gene regulation in higher eukaryotes. However, the boundaries of promoter regions, the relative rates of initiation at the transcription start sites (TSSs) distributed within them, and the functional significance of promoter architecture remain poorly understood. We produced a high-resolution map of promoters active in the Drosophila melanogaster embryo by integrating data from three independent and complementary methods: 21 million cap analysis of gene expression (CAGE) tags, 1.2 million RNA ligase mediated rapid amplification of cDNA ends (RLMRACE) reads, and 50,000 cap-trapped expressed sequence tags (ESTs). We defined 12,454 promoters of 8037 genes. Our analysis indicates that, due to non-promoter-associated RNA background signal, previous studies have likely overestimated the number of promoter-associated CAGE clusters by fivefold. We show that TSS distributions form a complex continuum of shapes, and that promoters active in the embryo and adult have highly similar shapes in 95% of cases. This suggests that these distributions are generally determined by static elements such as local DNA sequence and are not modulated by dynamic signals such as histone modifications. Transcription factor binding motifs are differentially enriched as a function of promoter shape, and peaked promoter shape is correlated with both temporal and spatial regulation of gene expression. Our results contribute to the emerging view that core promoters are functionally diverse and control patterning of gene expression in Drosophila and mammals.

  20. German Wind Energy Association | Open Energy Information

    Open Energy Info (EERE)

    German Wind Energy Association Place: Osnabrck, Germany Zip: 49074 Sector: Wind energy Product: Assocation for the promotion of wind energy in Germany. References: German Wind...

  1. US Integrated Waste Services Association | Open Energy Information

    Open Energy Info (EERE)

    Integrated Waste Services Association Jump to: navigation, search Name: US Integrated Waste Services Association Place: Washington, DC Product: Focussed on promoting integrated...

  2. Crystallographic Association Fellow

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    named American Crystallographic Association Fellow January 4, 2013 The American Crystallographic Association (ACA) honored Thomas Terwilliger of LANL's Biosecurity and Public Health group with the rank of Fellow. He was inducted during the ACA annual meeting in Boston. The ACA Fellows program celebrates the excellence of its members and promotes their recognition worldwide. ACA Fellows serve as scientific ambassadors to the broader scientific community and the general public to advance science

  3. Promoters and proteins from Clostridium thermocellum and uses thereof

    DOE Patents [OSTI]

    Wu, J. H. David; Newcomb, Michael

    2012-11-13

    The present invention relates to an inducible and a high expression nucleic acid promoter isolated from Clostridium thermocellum. These promoters are useful for directing expression of a protein or polypeptide encoded by a nucleic acid molecule operably associated with the nucleic acid promoters. The present invention also relates to nucleic acid constructs including the C. thermocellum promoters, and expression vectors and hosts containing such nucleic acid constructs. The present invention also relates to protein isolated from Clostridium thermocellum, including a repressor protein. The present invention also provides methods of using the isolated promoters and proteins from Clostridium thermocellum, including methods for directing inducible in vitro and in vivo expression of a protein or polypeptide in a host, and methods of producing ethanol from a cellulosic biomass.

  4. Secretary Bodman Promotes Energy Bill to Western Governors | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Promotes Energy Bill to Western Governors Secretary Bodman Promotes Energy Bill to Western Governors March 1, 2005 - 10:37am Addthis WASHINGTON, DC - U.S. Secretary of Energy Samuel W. Bodman in a speech before the Western Governors Association today expressed the need for Congress to pass comprehensive energy legislation and highlighted the benefits of the proposal for the western United States. Secretary Bodman also discussed a number of important energy initiatives including:

  5. ORISE: Iddins promoted to associate director of Radiation Emergency...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Prior to joining ORAU, Iddins served as a staff physician for the Methodist Medical Center Healthworks and Roane Industrial Medicine where she performed occupational health ...

  6. Photovoltaic module with adhesion promoter

    DOE Patents [OSTI]

    Xavier, Grace

    2013-10-08

    Photovoltaic modules with adhesion promoters and methods for fabricating photovoltaic modules with adhesion promoters are described. A photovoltaic module includes a solar cell including a first surface and a second surface, the second surface including a plurality of interspaced back-side contacts. A first glass layer is coupled to the first surface by a first encapsulating layer. A second glass layer is coupled to the second surface by a second encapsulating layer. At least a portion of the second encapsulating layer is bonded directly to the plurality of interspaced back-side contacts by an adhesion promoter.

  7. Cu(II) promotes amyloid pore formation

    SciTech Connect (OSTI)

    Zhang, Hangyu; Rochet, Jean-Christophe; Stanciu, Lia A.

    2015-08-14

    The aggregation of α-synuclein is associated with dopamine neuron death in Parkinson's disease. There is controversy in the field over the question of which species of the aggregates, fibrils or protofibrils, are toxic. Moreover, compelling evidence suggested the exposure to heavy metals to be a risk of PD. Nevertheless, the mechanism of metal ions in promoting PD remains unclear. In this research, we investigated the structural basis of Cu(II) induced aggregation of α-synuclein. Using transmission electron microscopy experiments, Cu(II) was found to promote in vitro aggregation of α-synuclein by facilitating annular protofibril formation rather than fibril formation. Furthermore, neuroprotective baicalein disaggregated annular protofibrils accompanied by considerable decrease of β-sheet content. These results strongly support the hypothesis that annular protofibrils are the toxic species, rather than fibrils, thereby inspiring us to search novel therapeutic strategies for the suppression of the toxic annular protofibril formation. - Highlights: • Cu(II) promoted the annular protofibril formation of α-synuclein in vitro. • Cu(II) postponed the in vitro fibrillization of α-synuclein. • Neuroprotective baicalein disaggregated annular protofibrils.

  8. Consumer Electronics Association Comment | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Comment Consumer Electronics Association Comment The Consumer Electronics Association (CEA) is the preeminent trade association promoting growth in the $285 billion U.S. consumer electronics industry. CEA comments re DOE Regulatory Burden RFI_7-17-15 (164.06 KB) More Documents & Publications Consumer Electronics Association Comments

  9. Blog Promotion | Open Energy Information

    Open Energy Info (EERE)

    and are interested in blogging, you may want to promote your blog using OpenEI's social media and rss feed channels. Here is a simple list of information on how to have...

  10. Initiation Complex Structure and Promoter Proofreading (Journal...

    Office of Scientific and Technical Information (OSTI)

    Initiation Complex Structure and Promoter Proofreading Citation Details In-Document Search Title: Initiation Complex Structure and Promoter Proofreading Authors: Liu, Xin ; ...

  11. Renewable Fuels Assocation | Open Energy Information

    Open Energy Info (EERE)

    DC Zip: 20001 Sector: Renewable Energy Product: US national trade association for the ethanol industry, the Renewable Fuels Association (RFA) has been working as the "Voice of the...

  12. Tamil Nadu Spinning Mills Association TASMA | Open Energy Information

    Open Energy Info (EERE)

    Tamil Nadu, India Zip: 624003 Sector: Wind energy Product: Association of spinning mill owners; promoting wind power projects. References: Tamil Nadu Spinning Mills...

  13. RFA-14-0002 - In the Matter of Highway Oil, Inc. | Department of Energy

    Energy Savers [EERE]

    REVISED MD-110 REFERENCE GUIDE SEPTEMBER 2015 BACKGROUND On August 5, 2015, the Equal Employment Opportunity Commission (EEOC) approved the first revision to its Management Directive 110 (MD-110) since 1999. The revised MD-110 provides federal agencies with updated Commission policies, procedures, and guidance relating to the federal sector complaint process as set forth in 29 C.F.R. Part 1614 and reflects new developments in case law, as the federal workplace and EEO practices have evolved. The

  14. Students' Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Resources Living in Los Alamos Ombuds Program Scholarships Student Association Autobiographies Student Programs Advisory Committee (internal) 2015 Student Liaison Contact List

  15. Students' Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Living in Los Alamos Ombuds Program Scholarships Student Association Autobiographies Student Programs Advisory Committee (internal) 2015 Student Liaison Contact List (pdf)

  16. How Does Your Community Promote Energy Savings?

    Broader source: Energy.gov [DOE]

    John works with various organizations in his community to promote energy savings, and he has found that none of these organizations can do it alone.

  17. Utility Promoters for Biomass Feedstock Biotechnology - Energy...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Utility Promoters for Biomass Feedstock Biotechnology Inventors: Kyung-Hwan Han, Jae-Heung ... Successful application of biotechnology requires both gene discovery and a proper means ...

  18. Consumer Electronics Association Comments | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Comments Consumer Electronics Association Comments The Consumer Electronics Association (CEA) is the preeminent trade association promoting growth in the $285 billion U.S. consumer electronics industry. CEA represents more than 2,000 corporate members involved in the design, development, manufacturing, distribution and integration of audio, video, in-vehicle electronics, wireless and landline communications, information technology, home networking, multimedia and accessory products, as well as

  19. Statistical Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Kelly named Fellow of the American Statistical Association August 2, 2016 The American Statistical Association (ASA) has honored Elizabeth Kelly of the Lab's Statistical Sciences group with the title of Fellow. The ASA recognized her for providing exemplary statistical leadership of and collaboration on multidisciplinary teams dealing with environmental restoration, weapon quality, and nuclear materials storage to ensure the safety and security of the Nation. She will receive the Fellow award at

  20. Obama Officials Announce Steps to Promote the Clean Energy Potential of the

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    West | Department of Energy Obama Officials Announce Steps to Promote the Clean Energy Potential of the West Obama Officials Announce Steps to Promote the Clean Energy Potential of the West June 15, 2009 - 4:03pm Addthis Secretaries Chu, Salazar and Vilsack and Chairs Sutley and Wellinghoff announce energy and transmission policies at the Western Governors' Association Annual Meeting PARK CITY, UTAH - Senior Obama Administration officials today announced a number of steps that will help the

  1. DOE Launches EnergySmart Hospitals to Promote Improved Energy Efficiency in

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Healthcare | Department of Energy EnergySmart Hospitals to Promote Improved Energy Efficiency in Healthcare DOE Launches EnergySmart Hospitals to Promote Improved Energy Efficiency in Healthcare July 23, 2008 - 2:14pm Addthis Initiative Targets Energy and Cost Savings in Hospitals Across the United States WASHINGTON - U.S. Department of Energy (DOE) Associate Under Secretary for Energy Richard F. Moorer today announced the launch of the EnergySmart Hospitals initiative to increase the use of

  2. Workplace Charging Challenge: Promote Charging at Work

    Broader source: Energy.gov [DOE]

    Employees with access to workplace charging are six times more likely to drive a plug-in electric vehicle (PEV) than the average worker. Promoting PEV charging at workplaces is one great way that...

  3. Extracellular Proteins Promote Zinc Sulfide Aggregation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Extracellular Proteins Promote Zinc Sulfide Aggregation Extracellular Proteins Promote Zinc Sulfide Aggregation Print Wednesday, 26 September 2007 00:00 Researchers from the ALS, Berkeley Lab's National Center for Electron Microscopy (NCEM), and Lawrence Livermore National Laboratory analyzed biofilm samples rich in zinc sulfide and dominated by sulfate-reducing bacteria, which were collected from lead-zinc mine waters. The researchers were curious about the relationship of the organic material

  4. Alternative Fuels Data Center: San Diego Leads in Promoting EVs

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    San Diego Leads in Promoting EVs to someone by E-mail Share Alternative Fuels Data Center: San Diego Leads in Promoting EVs on Facebook Tweet about Alternative Fuels Data Center: San Diego Leads in Promoting EVs on Twitter Bookmark Alternative Fuels Data Center: San Diego Leads in Promoting EVs on Google Bookmark Alternative Fuels Data Center: San Diego Leads in Promoting EVs on Delicious Rank Alternative Fuels Data Center: San Diego Leads in Promoting EVs on Digg Find More places to share

  5. Energy and EPA Webinar Series Promotes Tribal Energy Sufficiency...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Promotes Tribal Energy Sufficiency and Economic Development Energy and EPA Webinar Series Promotes Tribal Energy Sufficiency and Economic Development January 20, 2012 - 11:40am ...

  6. Energy Portfolio Standards and the Promotion of Combined Heat...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Portfolio Standards and the Promotion of Combined Heat and Power (CHP) White Paper, April 2009 Energy Portfolio Standards and the Promotion of Combined Heat and Power (CHP) White ...

  7. Optimized scalar promotion with load and splat SIMD instructions...

    Office of Scientific and Technical Information (OSTI)

    Optimized scalar promotion with load and splat SIMD instructions Citation Details In-Document Search Title: Optimized scalar promotion with load and splat SIMD instructions ...

  8. National Programme for the Promotion of Solar Energy | Open Energy...

    Open Energy Info (EERE)

    Programme for the Promotion of Solar Energy Jump to: navigation, search Name National Programme for the Promotion of Solar Energy AgencyCompany Organization Deutsche Gesellschaft...

  9. Centre for Jatropha Promotion Biodiesel | Open Energy Information

    Open Energy Info (EERE)

    Jatropha Promotion Biodiesel Jump to: navigation, search Name: Centre for Jatropha Promotion & Biodiesel Place: Churu, Rajasthan, India Zip: 331001 Product: Indian-based non-profit...

  10. Energy 101: Promoting Energy Education in the Nation's Colleges...

    Office of Environmental Management (EM)

    Energy 101: Promoting Energy Education in the Nation's Colleges and Universities Energy 101: Promoting Energy Education in the Nation's Colleges and Universities June 25, 2014 - ...

  11. Policies for Promoting Industrial Energy Efficiency in Developing...

    Open Energy Info (EERE)

    Promoting Industrial Energy Efficiency in Developing Countries and Transition Economies Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Policies for Promoting Industrial...

  12. Department of Energy Announces $15 Million to Promote Innovative...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    15 Million to Promote Innovative Geothermal Heat Recovery Methods and Technologies Department of Energy Announces 15 Million to Promote Innovative Geothermal Heat Recovery...

  13. United States, Australia, and Iceland to Promote Geothermal Energy...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    United States, Australia, and Iceland to Promote Geothermal Energy United States, Australia, and Iceland to Promote Geothermal Energy August 28, 2008 - 12:43pm Addthis Photo of ...

  14. Promoting a Green Economy through Clean Transportation Alternatives...

    Broader source: Energy.gov (indexed) [DOE]

    KB) More Documents & Publications Promoting a Green Economy through Clean Transportation Alternatives Promoting a Green Economy through Clean Transportation Alternatives EV ...

  15. Promoting a Green Economy through Clean Transportation Alternatives...

    Broader source: Energy.gov (indexed) [DOE]

    Promoting a Green Economy through Clean Transportation Alternatives Promoting a Green Economy through Clean Transportation Alternatives Town of Hempstead: Project Energy, From ...

  16. Shuchi Energy Ad Promotions P Ltd | Open Energy Information

    Open Energy Info (EERE)

    Shuchi Energy Ad Promotions P Ltd Jump to: navigation, search Name: Shuchi Energy Ad Promotions (P) Ltd Place: Hyderabad, Andhra Pradesh, India Zip: 500062 Sector: Solar Product:...

  17. PRESS RELEASE: Community Leaders Institutes (CLIs) Promote Awareness...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PRESS RELEASE: Community Leaders Institutes (CLIs) Promote Awareness of Climate Change Impacts PRESS RELEASE: Community Leaders Institutes (CLIs) Promote Awareness of Climate Change ...

  18. UNIDO Investment and Technology Promotion | Open Energy Information

    Open Energy Info (EERE)

    Investment and Technology Promotion Jump to: navigation, search Name UNIDO Investment and Technology Promotion AgencyCompany Organization United Nations Industrial Development...

  19. Vega Biofuels Inc formerly Vega Promotional Systems | Open Energy...

    Open Energy Info (EERE)

    Vega Biofuels Inc formerly Vega Promotional Systems Jump to: navigation, search Name: Vega Biofuels Inc (formerly Vega Promotional Systems) Place: Norcross, Georgia Zip: 30010...

  20. Environmental Law Institute Webinar to Promote Superior Energy...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Environmental Law Institute Webinar to Promote Superior Energy Performance(tm) on July 22, 2014 Environmental Law Institute Webinar to Promote Superior Energy Performance(tm) on ...

  1. Universal light-switchable gene promoter system

    DOE Patents [OSTI]

    Quail, Peter H.; Huq, Enamul; Tepperman, James; Sato, Sae

    2005-02-22

    An artificial promoter system that can be fused upstream of any desired gene enabling reversible induction or repression of the expression of the gene at will in any suitable host cell or organisms by light is described. The design of the system is such that a molecule of the plant photoreceptor phytochrome is targeted to the specific DNA binding site in the promoter by a protein domain that is fused to the phytochrome and that specifically recognizes this binding site. This bound phytochrome, upon activation by light, recruits a second fusion protein consisting of a protein that binds to phytochrome only upon light activation and a transcriptional activation domain that activates expression of the gene downstream of the promoter.

  2. Promotion of Efficient Use of Energy

    SciTech Connect (OSTI)

    Harry Misuriello; DOE Project Officer - Keith Bennett

    2006-01-25

    The Department of Energy funded the Alliance to Save Energy to promote the efficient use of energy under a multiyear cooperative agreement. This funding allowed the Alliance to be innovative and flexible in its program development, and to initiate and enhance projects it would otherwise not have been able to pursue. The program period was 1999 through 2004. The mission of the Alliance to Save Energy is to promote energy efficiency domestically and worldwide. The Alliance followed this mission by working closely with consumers, government, policy makers, and energy efficient product and service providers. The projects that were initiated by the Alliance included communication and consumer education, policy analysis and research, the promotion of interaction among the energy efficiency industry, and international energy efficiency programs. The funding from the Department of Energy allowed the Alliance to study new issues in energy efficiency, draw public attention to those issues, and create targeted programs, such as the Efficient Windows Collaborative or the Green Schools program, which now function on their own to promote energy efficiency in important areas.

  3. Method for promoting Michael addition reactions

    DOE Patents [OSTI]

    Shah, Pankaj V.; Vietti, David E.; Whitman, David William

    2010-09-21

    Homogeneously dispersed solid reaction promoters having an average particle size from 0.01 .mu.m to 500 .mu.m are disclosed for preparing curable mixtures of at least one Michael donor and at least one Michael acceptor. The resulting curable mixtures are useful as coatings, adhesives, sealants and elastomers.

  4. Promoting energy efficiency in reforming electricity markets

    SciTech Connect (OSTI)

    Clinton, J.; Kozloff, K.

    1998-07-01

    Many developing countries are initiating power sector reforms to stimulate private investment, increase operation and management efficiencies, and recover the full costs of power. Reforms may include unbundling generation, transmission, distribution and retail services; commercial management; competition; and private ownership. This paper draws upon six country case studies--Argentina, Chile, New Zealand, Norway, the United Kingdom, and the US--to identify major models of power reforms and their implications for energy efficiency--both positive and negative. There are both structural and institutional features of reform that may discourage commercial offerings of end-use efficiency services. Valuable lessons are discussed regarding what reforms and policies have worked to promote energy efficiency and which have not. Several models are offered for how developing countries can promote energy efficiency under some of the more common forms of power sector restructuring. Conclusions and recommendations are directed at key decision-makers in developing countries contemplating power sector reforms.

  5. Extracellular Proteins Promote Zinc Sulfide Aggregation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Extracellular Proteins Promote Zinc Sulfide Aggregation Print Researchers from the ALS, Berkeley Lab's National Center for Electron Microscopy (NCEM), and Lawrence Livermore National Laboratory analyzed biofilm samples rich in zinc sulfide and dominated by sulfate-reducing bacteria, which were collected from lead-zinc mine waters. The researchers were curious about the relationship of the organic material and metals, particularly how organics affect mobility, and its potential for

  6. Extracellular Proteins Promote Zinc Sulfide Aggregation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Extracellular Proteins Promote Zinc Sulfide Aggregation Print Researchers from the ALS, Berkeley Lab's National Center for Electron Microscopy (NCEM), and Lawrence Livermore National Laboratory analyzed biofilm samples rich in zinc sulfide and dominated by sulfate-reducing bacteria, which were collected from lead-zinc mine waters. The researchers were curious about the relationship of the organic material and metals, particularly how organics affect mobility, and its potential for

  7. Extracellular Proteins Promote Zinc Sulfide Aggregation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Extracellular Proteins Promote Zinc Sulfide Aggregation Print Researchers from the ALS, Berkeley Lab's National Center for Electron Microscopy (NCEM), and Lawrence Livermore National Laboratory analyzed biofilm samples rich in zinc sulfide and dominated by sulfate-reducing bacteria, which were collected from lead-zinc mine waters. The researchers were curious about the relationship of the organic material and metals, particularly how organics affect mobility, and its potential for

  8. Extracellular Proteins Promote Zinc Sulfide Aggregation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Extracellular Proteins Promote Zinc Sulfide Aggregation Print Researchers from the ALS, Berkeley Lab's National Center for Electron Microscopy (NCEM), and Lawrence Livermore National Laboratory analyzed biofilm samples rich in zinc sulfide and dominated by sulfate-reducing bacteria, which were collected from lead-zinc mine waters. The researchers were curious about the relationship of the organic material and metals, particularly how organics affect mobility, and its potential for

  9. Extracellular Proteins Promote Zinc Sulfide Aggregation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Extracellular Proteins Promote Zinc Sulfide Aggregation Print Researchers from the ALS, Berkeley Lab's National Center for Electron Microscopy (NCEM), and Lawrence Livermore National Laboratory analyzed biofilm samples rich in zinc sulfide and dominated by sulfate-reducing bacteria, which were collected from lead-zinc mine waters. The researchers were curious about the relationship of the organic material and metals, particularly how organics affect mobility, and its potential for

  10. Extracellular Proteins Promote Zinc Sulfide Aggregation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Extracellular Proteins Promote Zinc Sulfide Aggregation Print Researchers from the ALS, Berkeley Lab's National Center for Electron Microscopy (NCEM), and Lawrence Livermore National Laboratory analyzed biofilm samples rich in zinc sulfide and dominated by sulfate-reducing bacteria, which were collected from lead-zinc mine waters. The researchers were curious about the relationship of the organic material and metals, particularly how organics affect mobility, and its potential for

  11. Promoting Sustainability on a Global Scale

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Promoting Sustainability on a Global Scale Biomass 2013 Washington, 1 August 2013 Martina Otto Head of Policy Unit, Energy Branch United Nations Environment Programme www.unep.org/energy/bioenergy UNEP Global Environment Outlook - GEO-5: We are already observing changes to the Earth System, unprecedented in human history. Efforts to slow the rate or extent of change have resulted in moderate successes but have not succeeded in reversing adverse environmental changes. We need transformational

  12. Obama Officials Announce Steps to Promote the Clean Energy Potential...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Obama Officials Announce Steps to Promote the Clean Energy Potential of the West Obama Officials Announce Steps to Promote the Clean Energy Potential of the West June 15, 2009 -...

  13. Active hopper for promoting flow of bulk granular or powdered...

    Office of Scientific and Technical Information (OSTI)

    Data Explorer Search Results Active hopper for promoting flow of bulk granular or powdered ... An apparatus that promotes the flow of materials has a body having an inner shape for ...

  14. Workplace Charging Challenge: Promote Charging at Work | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Promote Charging at Work Workplace Charging Challenge: Promote Charging at Work Workplace Charging Challenge: Promote Charging at Work Employees with access to workplace charging are six times more likely to drive a plug-in electric vehicle (PEV) than the average worker. Promoting PEV charging at workplaces is one great way that states, cities and other organizations can encourage PEV adoption in their communities. Use the material below to engage and educate employers about the

  15. DOE Promotion of ISO 50001 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ISO 50001 Energy Management Standard » DOE Promotion of ISO 50001 DOE Promotion of ISO 50001 The U.S. Department of Energy (DOE) played a critical role in developing ISO 50001-the world's first global energy management system standard. ISO 50001 promotes the widespread adoption of best practices and drives investment in energy efficiency. DOE actively supports its broad implementation to achieve powerful benefits to U.S. businesses and to the nation. Learn how various DOE offices are promoting

  16. Method for using a yeast alpha-amylase promoter

    DOE Patents [OSTI]

    Gao, Johnway; Skeen, Rodney S.; Hooker, Brian S.; Anderson, Daniel B.

    2003-04-22

    The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

  17. RFA-14-0001- In the Matter of Commonwealth Oil Refining Company, Inc./Commonwealth of Puerto Rico

    Broader source: Energy.gov [DOE]

    On July 29, 2014, OHA approved a distribution plan submitted by the Commonwealth of Puerto Rico (Puerto Rico) to use approximately $1.2 million of petroleum overcharge funds held in escrow by the...

  18. Diesel Engine CO2 and SOx Emission Compliance Strategy for the Royal Navy (RN) and Royal Fleet Auxiliary (RFA) Flotillas

    Broader source: Energy.gov [DOE]

    Poster presentation from the 2007 Diesel Engine-Efficiency & Emissions Research Conference (DEER 2007). 13-16 August, 2007, Detroit, Michigan. Sponsored by the U.S. Department of Energy's (DOE) Office of FreedomCAR and Vehicle Technologies (OFCVT).

  19. Postdocs - Berkeley Lab Postdoc Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Postdocs - Berkeley Lab Postdoc Association

  20. Brookhaven Science Associates, LLC

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Doon L. Gibbs Laboratory Director Brookhaven Science Associates, LLC Brookhaven National ... on November 12, 2014. Brookhaven Science Associates, LLC (BSA) documented this ...

  1. MRG15 activates the cdc2 promoter via histone acetylation in human cells

    SciTech Connect (OSTI)

    Pena, AndreAna N.; Tominaga, Kaoru; Pereira-Smith, Olivia M.; Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, TX

    2011-07-01

    Chromatin remodeling is required for transcriptional activation and repression. MRG15 (MORF4L1), a chromatin modulator, is a highly conserved protein and is present in complexes containing histone acetyltransferases (HATs) as well as histone deacetylases (HDACs). Loss of expression of MRG15 in mice and Drosophila results in embryonic lethality and fibroblast and neural stem/progenitor cells cultured from Mrg15 null mouse embryos exhibit marked proliferative defects when compared with wild type cells. To determine the role of MRG15 in cell cycle progression we performed chromatin immunoprecipitation with an antibody to MRG15 on normal human fibroblasts as they entered the cell cycle from a quiescent state, and analyzed various cell cycle gene promoters. The results demonstrated a 3-fold increase in MRG15 occupancy at the cdc2 promoter during S phase of the cell cycle and a concomitant increase in acetylated histone H4. H4 lysine 12 was acetylated at 24 h post-serum stimulation while there was no change in acetylation of lysine 16. HDAC1 and 2 were decreased at this promoter during cell cycle progression. Over-expression of MRG15 in HeLa cells activated a cdc2 promoter-reporter construct in a dose-dependent manner, whereas knockdown of MRG15 resulted in decreased promoter activity. In order to implicate HAT activity, we treated cells with the HAT inhibitor anacardic acid and determined that HAT inhibition results in loss of expression of cdc2 mRNA. Further, chromatin immunoprecipitation with Tip60 localizes the protein to the same 110 bp stretch of the cdc2 promoter pulled down by MRG15. Additionally, we determined that cotransfection of MRG15 with the known associated HAT Tip60 had a cooperative effect in activating the cdc2 promoter. These results suggest that MRG15 is acting in a HAT complex involving Tip60 to modify chromatin via acetylation of histone H4 at the cdc2 promoter to activate transcription.

  2. Promoting Sustainability on a Global Scale | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Promoting Sustainability on a Global Scale Promoting Sustainability on a Global Scale Promoting Sustainability on a Global Scale Martina Otto, Head of the Policy Unit, United Nations Environment Programme b13_otto_sustianability.pdf (715.08 KB) More Documents & Publications Sustainability for the Global Biofuels Industry Minimizing Risks and Maximizing Opportunities Sustainability for the Global Biofuels Industry: Minimizing Risks and Maximizing Opportunities Bioenergy Technologies Office

  3. State and Regional policies that promote energy efficiency programs carried

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    out by electric and gas utilities | Department of Energy and Regional policies that promote energy efficiency programs carried out by electric and gas utilities State and Regional policies that promote energy efficiency programs carried out by electric and gas utilities A report to the United States Congress Pursuant to section 139 of the energy policy act of 2005. March 2007 State and Regional policies that promote energy efficiency programs carried out by electric and gas utilities (1.42

  4. State and regional policies that promote energy efficiency programs carried

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    out by electric and gas utilities | Department of Energy and regional policies that promote energy efficiency programs carried out by electric and gas utilities State and regional policies that promote energy efficiency programs carried out by electric and gas utilities A report to the United States Congress Pursuant to section 139 of the Energy Policy Act of 2005. March 2007 State and regional policies that promote energy efficiency programs carried out by electric and gas utilities (1.42

  5. Cybersecurity Awareness Marketing/Promotional Material | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Marketing/Promotional Material Cybersecurity Awareness Marketing/Promotional Material The OCIO has developed a variety of cyber awareness marketing and promotional materials available for download to DOE organizations, and public and private institutions. 2013 NCSAM Announcement (94.49 KB) Supply Chain Risk Management Awareness (50.09 KB) A Case of Mistaken Identity Communication (148.91 KB) 2012 NCSAM Postcards (152.1 KB) A Case of Mistaken Identity Word Search (182.8 KB) 2012 NCSAM

  6. Publicly Backed Guarantees as Policy Instruments to Promote Clean...

    Open Energy Info (EERE)

    Publicly Backed Guarantees as Policy Instruments to Promote Clean Energy Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Publicly Backed Guarantees as Policy Instruments...

  7. Senegal-GTZ Programme to Promote Rural Electrification and a...

    Open Energy Info (EERE)

    in producing and marketing their products, and also promotes companies which produce alternative fuels from waste materials and reeds. This lightens the workload of women, who...

  8. Investigations of element spatial correlation in Mn-promoted...

    Office of Scientific and Technical Information (OSTI)

    Investigations of element spatial correlation in Mn-promoted Co-based Fischer-Tropsch synthesis catalysts This content will become publicly available on June 4, 2017 Title: ...

  9. State and regional policies that promote energy efficiency programs...

    Broader source: Energy.gov (indexed) [DOE]

    State and Regional policies that promote energy efficiency programs carried out by electric and gas utilities Chapter 3: Demand-Side Resources Benefits of Demand Response in ...

  10. State and Regional policies that promote energy efficiency programs...

    Office of Environmental Management (EM)

    State and regional policies that promote energy efficiency programs carried out by electric and gas utilities Chapter 3: Demand-Side Resources Benefits of Demand Response in ...

  11. Solar Promotion International GmbH | Open Energy Information

    Open Energy Info (EERE)

    International GmbH Place: Pforzheim, Germany Zip: 75172 Sector: Solar Product: German organiser of global solar conferences. References: Solar Promotion International...

  12. Optimized scalar promotion with load and splat SIMD instructions...

    Office of Scientific and Technical Information (OSTI)

    Search Title: Optimized scalar promotion with load and splat SIMD instructions Mechanisms for optimizing scalar code executed on a single instruction multiple data (SIMD) engine ...

  13. Inhibition of Sirt1 promotes neural progenitors toward motoneuron...

    Office of Scientific and Technical Information (OSTI)

    Sirt1, to promote neural precursor cell (NPC) development during differentiation ... in the development of hESC-based cell therapy in motoneuron disease. less ...

  14. Nepali Alternative Energy Promotion Centre (AEPC) | Open Energy...

    Open Energy Info (EERE)

    is a Government institution established on November 3, 1996 under the then Ministry of Science and Technology with the objective of developing and promoting renewablealternative...

  15. A Model for the Nation: Promoting Education and Innovation in...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Model for the Nation: Promoting Education and Innovation in Vermont's Electricity Sector - ... Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy ...

  16. Building Bridges: Federal Agencies Join Forces to Promote Sustainable...

    Broader source: Energy.gov (indexed) [DOE]

    tribal communities by: 1. Promoting sustainable economic development; 2. Supporting ... and 5. Protecting and supporting the sustainable management of Native lands, ...

  17. Secretary Chu Announces New Efforts to Promote Clean Energy in...

    Broader source: Energy.gov (indexed) [DOE]

    Up to 10 million will be available this year through DOE's Tribal Energy Program to support ... renewable energy resources, and promote economic development for tribal communities. ...

  18. Ghana-GTZ Electrification Component of the Promotion of Private...

    Open Energy Info (EERE)

    Topics Background analysis Website http:www.gtz.deenthemenum Country Ghana Western Africa References Electrification Component of the Promotion of Private Sector Programme...

  19. Top 15 Ways to Promote PEVs at Work

    Broader source: Energy.gov [DOE]

    Your decision to offer workplace charging can increase PEV awareness among current and potential employees. Consider organizing the activities below to promote workplace charging.

  20. South Africa-Promoting Low Emission Urban Development Strategies...

    Open Energy Info (EERE)

    search Name South Africa-Promoting Low Emission Urban Development Strategies in Emerging Economy Countries (URBAN-LEDS) AgencyCompany Organization ICLEI - Local Governments for...

  1. The oncoprotein HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote the proliferation of breast cancer cells

    SciTech Connect (OSTI)

    Zhang, Yingyi; Zhao, Yu; Li, Leilei; Shen, Yu; Cai, Xiaoli; Zhang, Xiaodong; Ye, Lihong

    2013-05-03

    Highlights: HBXIP is able to upregulate the expression of PDGFB in breast cancer cells. HBXIP serves as a coactivator of activating transcription factor Sp1. HBXIP stimulates the PDGFB promoter via activating transcription factor Sp1. HBXIP promotes the proliferation of breast cancer cell via upregulating PDGFB. -- Abstract: We have reported that the oncoprotein hepatitis B virus X-interacting protein (HBXIP) acts as a novel transcriptional coactivator to promote proliferation and migration of breast cancer cells. Previously, we showed that HBXIP was able to activate nuclear factor-?B (NF-?B) in breast cancer cells. As an oncogene, the platelet-derived growth factor beta polypeptide (PDGFB) plays crucial roles in carcinogenesis. In the present study, we found that both HBXIP and PDGFB were highly expressed in breast cancer cell lines. Interestingly, HBXIP was able to increase transcriptional activity of NF-?B through PDGFB, suggesting that HBXIP is associated with PDGFB in the cells. Moreover, HBXIP was able to upregulate PDGFB at the levels of mRNA, protein and promoter in the cells. Then, we identified that HBXIP stimulated the promoter of PDGFB through activating transcription factor Sp1. In function, HBXIP enhanced the proliferation of breast cancer cells through PDGFB in vitro. Thus, we conclude that HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote proliferation of breast cancer cells.

  2. Regulatory Promotion of Emergent CCS Technology

    SciTech Connect (OSTI)

    Davies, Lincoln; Uchitel, Kirsten; Johnson, David

    2014-01-01

    Despite the growing inevitability of climate change and the attendant need for mitigation strategies, carbon capture and sequestration (CCS) has yet to gain much traction in the United States. Recent regulatory proposals by the U.S. Environmental Protection Agency (EPA), limited in scope to new-build power plants, represent the only significant policy initiative intended to mandate diffusion of CCS technology. Phase I of this Project assessed barriers to CCS deployment as prioritized by the CCS community. That research concluded that there were four primary barriers: (1) cost, (2) lack of a carbon price, (3) liability, and (4) lack of a comprehensive regulatory regime. Phase II of this Project, as presented in this Report, assesses potential regulatory models for CCS and examines where those models address the hurdles to diffusing CCS technology identified in Phase I. It concludes (1) that a CCS-specific but flexible standard, such as a technology performance standard or a very particular type of market-based regulation, likely will promote CCS diffusion, and (2) that these policies cannot work alone, but rather, should be combined with other measures, such as liability limits and a comprehensive CCS regulatory regime.

  3. Open House 2016 Promotional Video | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Open House 2016 Promotional Video Share Description Argonne Open House 2016 Promotional Video Browse By - Any - General Argonne Information Energy -Energy efficiency --Vehicles ---Alternative fuels ---Automotive engineering ---Diesel ---Electric drive technology ---Hybrid & electric vehicles ---Hydrogen & fuel cells ---Internal combustion ---Powertrain research --Building design ---Construction --Manufacturing -Energy sources --Renewable energy ---Bioenergy ---Solar energy --Fossil fuels

  4. Saline Infusion Markedly Reduces Impedance and Improves Efficacy of Pulmonary Radiofrequency Ablation

    SciTech Connect (OSTI)

    Gananadha, Sivakumar Morris, David Lawson

    2004-08-15

    Radiofrequency ablation (RFA) is a relatively new technique that has been investigated for the treatment of lung tumors. We evaluated for the first time the in vivo use of saline infusion during radiofrequency ablation of sheep lung. We performed RFA on 5 sheep using open and closed chest RFA and the RITA starburst XL and Xli probes using saline infusion with the Xli probe. The impedance and volume of ablation were compared. A total of 16 ablations were produced, 5 percutaneously and 11 open. The impedance during percutaneous and open RFA without saline infusion was 110 {+-} 16.2 and 183.3 {+-} 105.8 O, respectively. With the saline infusion the impedance was 71.3 {+-} 22O and 103.6 {+-} 37.5O. The effect of this was a significantly larger volume of ablation using the saline infusion during percutaneous RFA (90.6 {+-} 23 cm{sup 3} vs 10.47 {+-} 2.9 cm{sup 3}, p = 0.01) and open RFA (107.8 {+-} 25.8 cm{sup 3} vs 24.9 {+-} 19.3 cm{sup 3}, p = 0.0002). Saline infusion during RFA is associated with lower impedance, higher power delivery and larger lesion size.

  5. Bradbury Science Museum Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Museum Association Introducing Bradbury Science Museum Association The Bradbury Science Museum Association is a separate, not-for-profit entity envisioned to enhance the educational work of the museum. Contact Us thumbnail of Bradbury Science Museum (505) 667-4444 Email We welcome the Bradbury Science Museum Association as our partner in education. Join the BSMA and recieve all the benefits it provides. The pdf application is here. There are also Sponsorship and Business Partnerships available.

  6. Fusion Power Associates Awards

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    fpa awards Fusion Power Associates Awards Fusion Power Associates is "a non-profit, tax-exempt research and educational foundation, providing information on the status of fusion development and other applications of plasma science and fusion research". The Association makes awards in four categories: Distinguished Career Awards, Leadership Awards, Excellence in Fusion Engineering, and Special Awards. Since 1987, Distinguished Career Awards have been presented "to individuals who

  7. Brookhaven Science Associates, LLC

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    3, 2015 Dr. Doon L. Gibbs Laboratory Director Brookhaven Science Associates, LLC Brookhaven National Laboratory 40 Brookhaven Avenue Upton, New York 11973-5000 WCO-2015-02 Dear Dr. Gibbs: The Office of Enterprise Assessments' Office of Enforcement completed its investigation into the facts and circumstances associated with the meteorological tower electrical shock event that occurred at the Brookhaven National Laboratory on November 12, 2014. Brookhaven Science Associates, LLC (BSA) documented

  8. National Hydropower Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hydropower Association - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power ...

  9. Weapons Program Associate Directors

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    integration we have achieved between the various components of the program," said Bret Knapp, Principal Associate Director for Weapons Programs. "They have both done an...

  10. Added Public Promotional Page! | OpenEI Community

    Open Energy Info (EERE)

    Added Public Promotional Page Home > Groups > Linked Open Data Workshop in Washington, D.C. Jweers's picture Submitted by Jweers(88) Contributor 27 September, 2012 - 18:10 Event...

  11. Demolitions Produce Recyclable Materials for Organization Promoting Economic Activity

    Broader source: Energy.gov [DOE]

    Demolitions have helped generate more than 8 million pounds of metal at the Piketon site for recycling, further promoting economic activity in the region thanks to the American Recovery and...

  12. Forest County Potawatomi Tribe Cuts Emissions, Promotes Green...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Forest County Potawatomi Tribe Cuts Emissions, Promotes Green Growth February 23, 2012 - ... In pursuit of its long-term energy goal of reducing its carbon footprint to zero, the ...

  13. NIST approach to Promoting US Innovation and Industrial Competitiveness |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy NIST approach to Promoting US Innovation and Industrial Competitiveness NIST approach to Promoting US Innovation and Industrial Competitiveness Breakout Session 1: New Developments and Hot Topics Session 1-A: Biomass and the U.S. Competitive Advantages for Manufacturing Clean Energy Products Gary Anderson, Economist, NIST b13_anderson_1-a.pdf (811.66 KB) More Documents & Publications Benefit-Cost Evaluation of U.S. DOE Investment in Energy Storage Technologies for

  14. New Field Laboratories and Related Research To Help Promote Environmentally

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Prudent Development of Unconventional Resources | Department of Energy Field Laboratories and Related Research To Help Promote Environmentally Prudent Development of Unconventional Resources New Field Laboratories and Related Research To Help Promote Environmentally Prudent Development of Unconventional Resources November 6, 2014 - 9:00am Addthis WASHINGTON, D.C. - Today, the Department of Energy announced the selection of three multiyear, field laboratories and six other multiyear research

  15. Project Profile: Influence of Novel Behavioral Strategies in Promoting the

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Diffusion of Solar Energy | Department of Energy Soft Costs » Project Profile: Influence of Novel Behavioral Strategies in Promoting the Diffusion of Solar Energy Project Profile: Influence of Novel Behavioral Strategies in Promoting the Diffusion of Solar Energy Logos of Yale University, New York University Stern School of Business, SmartPower, Clean Energy Finance and Investment Authority, and Solarize Connecticut. A line graph that highlights the social interactions for accelerating

  16. Secretary Chu Announces Progress on International Initiatives to Promote

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Clean Energy | Department of Energy International Initiatives to Promote Clean Energy Secretary Chu Announces Progress on International Initiatives to Promote Clean Energy April 8, 2011 - 12:00am Addthis Washington, D.C. - U.S. Energy Secretary Steven Chu this week joined with energy ministers and high-level representatives from more than 20 governments to announce renewed support for 11 international clean energy initiatives at the second Clean Energy Ministerial in Abu Dhabi, United Arab

  17. Secretary Chu Announces Winners of Student Competition to Promote

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy-Efficient Buildings | Department of Energy Winners of Student Competition to Promote Energy-Efficient Buildings Secretary Chu Announces Winners of Student Competition to Promote Energy-Efficient Buildings March 5, 2012 - 1:03pm Addthis WASHINGTON, D.C. -- As part of President Obama's all-of-the-above energy strategy, Energy Secretary Steven Chu today announced the winners of a competition that challenges collegiate students to develop and present real-world solutions to boost the

  18. Energy 101: Promoting Energy Education in the Nation's Colleges and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Universities | Department of Energy Energy 101: Promoting Energy Education in the Nation's Colleges and Universities Energy 101: Promoting Energy Education in the Nation's Colleges and Universities June 25, 2014 - 2:46pm Addthis The Energy 101 initiative supports ongoing efforts in energy education. The first webinar of the Energy 101 dialogue series will focus on energy in the classroom. | National Renewable Energy Laboratory photo The Energy 101 initiative supports ongoing efforts in

  19. Promoting Combined Heat and Power (CHP) for Multifamily Properties, 2008 |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Promoting Combined Heat and Power (CHP) for Multifamily Properties, 2008 Promoting Combined Heat and Power (CHP) for Multifamily Properties, 2008 The U.S. Department of Housing and Urban Development (HUD) and the U.S. Department of Energy (DOE) and Oak Ridge National Laboratory (ORNL) developed preliminary feasibility (Level 1) screening software and enlisted the DOE CHP Regional Application Centers (RACs) to help run utility data and estimate paybacks. This paper

  20. Promoting NEPA Transparency and Public Engagement | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Promoting NEPA Transparency and Public Engagement Promoting NEPA Transparency and Public Engagement June 3, 2011 - 1:14pm Addthis "NEPA is, at its core, a transparency statute," said Katie Scharf, Council on Environmental Quality (CEQ) Deputy General Counsel, in opening a panel discussion on using information technology to support open government initiatives, engage the public, and add value to NEPA analysis. At the March 9, 2011, event - hosted by CEQ for Federal NEPA and legal staff

  1. Puget Sound Communities Promote Energy Efficiency | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Puget Sound Communities Promote Energy Efficiency Puget Sound Communities Promote Energy Efficiency April 2, 2010 - 4:50pm Addthis Lorelei Laird Writer, Energy Empowers What does this project do? Identifies wasted energy Frees up money for cash strapped programs in the area. Four organizations on Washington state's Kitsap Peninsula are joining forces to improve their energy efficiency. Led by the City of Bremerton, the largest participant city by population and energy needs, the four will hire

  2. DOE Announces $17 Million to Promote Greater Automobile Efficiency |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 7 Million to Promote Greater Automobile Efficiency DOE Announces $17 Million to Promote Greater Automobile Efficiency January 23, 2007 - 10:15am Addthis WASHINGTON, DC - U.S. Department of Energy (DOE) Assistant Secretary for Energy Efficiency and Renewable Energy Alexander Karsner today announced that DOE intends issue $17 million in solicitations to improve automobile efficiency and reduce the United States's dependence on foreign sources of oil. The funding will be

  3. New institute promotes nuclear security | Y-12 National Security Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New institute promotes ... New institute promotes nuclear security Posted: February 11, 2013 - 3:27pm | Y-12 Report | Volume 9, Issue 2 | 2013 Disquieting headlines from recent news reports prompt an important question: Who has the breadth of knowledge and experience to help solve international nuclear challenges? "A lot of the keys for national nuclear security are right here in Oak Ridge - from processing uranium at Y-12 to developing medical isotopes at Oak Ridge National Laboratory to

  4. Partnering with China to Promote Renewable Energy Deployment | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Partnering with China to Promote Renewable Energy Deployment Partnering with China to Promote Renewable Energy Deployment June 4, 2015 - 10:15am Addthis David Danielson, Assistant Secretary for Energy Efficiency and Renewable Energy, delivers keynote remarks at the fourth U.S.-China Renewable Energy Industries Forum in Washington, D.C. | <em>Photo by Josh Harmon</em> David Danielson, Assistant Secretary for Energy Efficiency and Renewable Energy, delivers keynote

  5. Fischer–Tropsch Synthesis: Characterization Rb Promoted Iron Catalyst

    SciTech Connect (OSTI)

    Sarkar,A.; Jacobs, G.; Ji, Y.; Hamdeh, H.; Davis, B.

    2008-01-01

    Rubidium promoted iron Fischer-Tropsch synthesis (FTS) catalysts were prepared with two Rb/Fe atomic ratios (1.44/100 and 5/100) using rubidium nitrate and rubidium carbonate as rubidium precursors. Results of catalytic activity and deactivation studies in a CSTR revealed that rubidium promoted catalysts result in a steady conversion with a lower deactivation rate than that of the corresponding unpromoted catalyst although the initial activity of the promoted catalyst was almost half that of the unpromoted catalyst. Rubidium promotion results in lower methane production, and higher CO2, alkene and 1-alkene fraction in FTS products. M{umlt o}ssbauer spectroscopic measurements of CO activated and working catalyst samples indicated that the composition of the iron carbide phase formed after carbidization was -Fe5 C2 for both promoted and unpromoted catalysts. However, in the case of the rubidium promoted catalyst, '-Fe2.2C became the predominant carbidic phase as FTS continued and the overall catalyst composition remained carbidic in nature. In contrast, the carbide content of the unpromoted catalyst was found to decline very quickly as a function of synthesis time. Results of XANES and EXAFS measurements suggested that rubidium was present in the oxidized state and that the compound most prevalent in the active catalyst samples closely resembled that of rubidium carbonate.

  6. Associative list processing unit

    DOE Patents [OSTI]

    Hemmert, Karl Scott; Underwood, Keith D.

    2013-01-29

    An associative list processing unit and method comprising employing a plurality of prioritized cell blocks and permitting inserts to occur in a single clock cycle if all of the cell blocks are not full. Also, an associative list processing unit and method comprising employing a plurality of prioritized cell blocks and using a tree of prioritized multiplexers descending from the plurality of cell blocks.

  7. Histone deacetylase inhibitors epigenetically promote reparative events in primary dental pulp cells

    SciTech Connect (OSTI)

    Duncan, Henry F.; Smith, Anthony J.; Fleming, Garry J.P.; Cooper, Paul R.

    2013-06-10

    Application of histone deacetylase inhibitors (HDACi) to cells epigenetically alters their chromatin structure and induces transcriptional and cellular reparative events. This study investigated the application of two HDACi, valproic acid (VPA) and trichostatin A (TSA) on the induction of repair-associated responses in primary dental pulp cell (DPC) cultures. Flow cytometry demonstrated that TSA (100 nM, 400 nM) significantly increased cell viability. Neither HDACi was cytotoxic, although cell growth analysis revealed significant anti-proliferative effects at higher concentrations for VPA (>0.5 mM) and TSA (>50 nM). While high-content-analysis demonstrated that HDACi did not significantly induce caspase-3 or p21 activity, p53-expression was increased by VPA (3 mM, 5 mM) at 48 h. HDACi-exposure induced mineralization per cell dose-dependently to a plateau level (VPA-0.125 mM and TSA-25 nM) with accompanying increases in mineralization/dentinogenic-associated gene expression at 5 days (DMP-1, BMP-2/-4, Nestin) and 10 days (DSPP, BMP-2/-4). Both HDACis, at a range of concentrations, significantly stimulated osteopontin and BMP-2 protein expression at 10 and 14 days further supporting the ability of HDACi to promote differentiation. HDACi exert different effects on primary compared with transformed DPCs and promote mineralization and differentiation events without cytotoxic effects. These novel data now highlight the potential in restorative dentistry for applying low concentrations of HDACi in vital pulp treatment. -- Highlights: • Valproic acid and trichostatin A promoted mineralization in primary pulp cells. • Cell viability, apoptosis, caspase-3, p21 unaltered; p53 increased by valproic acid. • Trichostatin A increased cell viability at 24 h at selected concentrations. • Altered cell toxicity and differentiation between primary and transformed cells. • HDACi-induced the differentiation marker proteins osteopontin and BMP-2.

  8. Isolated yeast promoter sequence and a method of regulated heterologous expression

    DOE Patents [OSTI]

    Gao, Johnway; Skeen, Rodney S.; Hooker, Brian S.; Anderson, Daniel B.

    2005-05-31

    The present invention provides the promoter clone discovery of a glucoamylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated glucoamylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

  9. Floyd Associates | Open Energy Information

    Open Energy Info (EERE)

    Associates Jump to: navigation, search Name: Floyd Associates Place: Beverly Hills, California Zip: 90210 Product: String representation "Floyd Associate ... GCC) countries." is...

  10. Media Promotes SEP Certification and DOE eGuide to Help Facilities...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Technical Assistance Superior Energy Performance Media Promotes SEP Certification and DOE eGuide to Help Facilities Reduce Energy Use and Costs Media Promotes SEP Certification ...

  11. Associative list processing unit

    DOE Patents [OSTI]

    Hemmert, Karl Scott; Underwood, Keith D

    2014-04-01

    An associative list processing unit and method comprising employing a plurality of prioritized cell blocks and permitting inserts to occur in a single clock cycle if all of the cell blocks are not full.

  12. Association Loan Program

    Broader source: Energy.gov [DOE]

    The homeowners' association should submit a proposal to AHFC for preliminary approval. The forms to start this loan process are located on the program web site. Loan limits are determined on a...

  13. Oak Ridge Associated Llniversities

    Office of Legacy Management (LM)

    ii!fil Prepared by Oak Ridge Associated Llniversities Prepared for U.S. Nuclear Regulatory Commission's Region I Office Supported by Safeguards dnd Materials Program Branch; Division of Quality Assurance, Safeguards, and Inspection Programs; Off ice of Inspection and r Enforcement I - CONFIRMATORY RADIOLOGICAL SURVEYS OF BUILDING 10 AND OUTSIDE AREAS ASSOCIATED WITH BUILDINGS 7 AND 8 WESTINGHOUSE NUCLEAR FUEL DIVISION CHESWICK, PENNSYLVANIA A. J. BOERNER Radiological Site Assessment Program

  14. ALS Users' Association Charter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Users' Executive Committee ALS Users' Association Charter Print The purpose of the Advanced Light Source Users' Association (ALSUA) is to provide an organized framework for the interaction between those who use the Advanced Light Source (ALS) at the Lawrence Berkeley Laboratory (LBL) for their research and the ALS management, as well as to provide a channel for communication with other synchrotron radiation laboratories and, on suitable occasions, with federal agencies. The ALSUA, representing

  15. Oak Ridge Associated

    Office of Legacy Management (LM)

    ])\D Oak Ridge ,.(\\~ Associated ru~ Universities Post Office Box 11 7 Oak Ridge, Tennessee 37831-0117 October 21, 1986 Manpower Education, Research, and Training Division Mr. Edward G. Delaney, Director Division of Facility and Site Decommissioning Projects Office of Nuclear Energy U.S. Department of Energy Washington, DC 20545 Subject: VERIFICATION OF NIAGARA FALLS STORAGE SITE VICINITY PROPERTIES - 1983/1984 REMEDIAL ACTIONS Dear Mr. Delaney: Oak Ridge Associated Universities <ORAU) has

  16. Bradbury Science Museum Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bradbury Science Museum Association 108 Central Park Square #208 Los Alamos, NM 87544 Dear Los Alamos National Laboratory Employee, member of the Los Alamos Community, or other friend of the Bradbury Science Museum, I am writing today to tell you about an exciting new opportunity! As of May 1, 2016, we are launching the Bradbury Science Museum Association (BSMA). Our purpose is to amplify the Bradbury Science Museum's (BSM) mission and visitor experience as its official nonprofit partner,

  17. Postdoc Association Committees

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Committees Postdoc Association Committees Addressing the needs of Los Alamos Lab postdocs. Contact Postdoc Program Office Email Dynamic committees address needs of the Lab's postdocs The Los Alamos Postdoc Association (LAPA) created a number of dynamic committees to accomplish specific goals that address the needs of postdocs. As a goal is achieved, a committee may be phased out; as new needs are recognized then new committees will be created to help meet those needs. Career Committee The Career

  18. ALS Users' Association Charter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Users' Association Charter Print The purpose of the Advanced Light Source Users' Association (ALSUA) is to provide an organized framework for the interaction between those who use the Advanced Light Source (ALS) at the Lawrence Berkeley Laboratory (LBL) for their research and the ALS management, as well as to provide a channel for communication with other synchrotron radiation laboratories and, on suitable occasions, with federal agencies. The ALSUA, representing the research workers, will be in

  19. ALS Users' Association Charter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Users' Association Charter Print The purpose of the Advanced Light Source Users' Association (ALSUA) is to provide an organized framework for the interaction between those who use the Advanced Light Source (ALS) at the Lawrence Berkeley Laboratory (LBL) for their research and the ALS management, as well as to provide a channel for communication with other synchrotron radiation laboratories and, on suitable occasions, with federal agencies. The ALSUA, representing the research workers, will be in

  20. ALS Users' Association Charter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ALS Users' Association Charter Print The purpose of the Advanced Light Source Users' Association (ALSUA) is to provide an organized framework for the interaction between those who use the Advanced Light Source (ALS) at the Lawrence Berkeley Laboratory (LBL) for their research and the ALS management, as well as to provide a channel for communication with other synchrotron radiation laboratories and, on suitable occasions, with federal agencies. The ALSUA, representing the research workers, will

  1. Organizations and associations serving the Waste-To-energy industry

    SciTech Connect (OSTI)

    Not Available

    1998-12-01

    Professional organizations can provide leadership in disseminating information and answering questions about, and in providing support for, the industry. Eleven such organizations and association that directly, or in part, promote or provide technical assistance in the waste-to-energy field are listed and described briefly. Some actively lobby on waste-to-energy issues. Some provide useful publications and newsletters for those interested in keeping up with changes in the field.

  2. The case for reasonable incentives to promote independent transmission companies

    SciTech Connect (OSTI)

    Angle, S.; Carpentier, D.A.; Farinella, M.

    1999-09-01

    As part of its restructuring efforts in the electric utility industry, the Federal Energy Regulatory Commission (FERC) has encouraged voluntary corporate unbundling in an effort to promote a competitive electric market, recently proposing to define the minimal and desired characteristics of a regional transmission organization (RTO). The promotion of competition requires certain features in a transmission system, which include inter alia, (1) independent operation; (2) efficient pricing of congestion; (3) congestion management; (4) provision of ancillary services; (5) elimination of pancaked transmission rates; (6) appropriate transmission pricing; and (7) price transparency. A promising type of corporate unbundling that can provide these features is an independent transmission company (ITC).

  3. GE Showcases Industrial Internet Innovations and Promotes Win-Win

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cooperation at 2015 TECHfest | GE Global Research Showcases Industrial Internet Innovations and Promotes Win-Win Cooperation at 2015 TECHfest Click to email this to a friend (Opens in new window) Share on Facebook (Opens in new window) Click to share (Opens in new window) Click to share on LinkedIn (Opens in new window) Click to share on Tumblr (Opens in new window) GE Showcases Industrial Internet Innovations and Promotes Win-Win Cooperation at 2015 TECHfest GE China Technology Center teams

  4. Scaffold architecture and fibrin gels promote meniscal cell proliferation

    SciTech Connect (OSTI)

    Pawelec, K. M. E-mail: jw626@cam.ac.uk; Best, S. M.; Cameron, R. E.; Wardale, R. J. E-mail: jw626@cam.ac.uk

    2015-01-01

    Stability of the knee relies on the meniscus, a complex connective tissue with poor healing ability. Current meniscal tissue engineering is inadequate, as the signals for increasing meniscal cell proliferation have not been established. In this study, collagen scaffold structure, isotropic or aligned, and fibrin gel addition were tested. Metabolic activity was promoted by fibrin addition. Cellular proliferation, however, was significantly increased by both aligned architectures and fibrin addition. None of the constructs impaired collagen type I production or triggered adverse inflammatory responses. It was demonstrated that both fibrin gel addition and optimized scaffold architecture effectively promote meniscal cell proliferation.

  5. Faster Tracks for Particle Accelerators Promoted by ODU Physicists (Inside

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ODU) | Jefferson Lab Faster Tracks for Particle Accelerators Promoted by ODU Physicists (Inside ODU) External Link: http://ww2.odu.edu/ao/ia/insideodu/20120426/topstory2.html By jlab_admin on Thu, 2012-04-2

  6. Savannah River Site Promotes Sharing Technology Innovation Across DOE Complex

    Broader source: Energy.gov [DOE]

    AIKEN, S.C. – An innovative data collection technology for environmental compliance sampling developed and used by the Savannah River Site is now being deployed at the Portsmouth Gaseous Diffusion Plant in Piketon, Ohio to increase the efficiency of cleanup operations as EM continues to promote sharing lessons learned and new technologies across DOE sites.

  7. United States, Australia, and Iceland to Promote Geothermal Energy

    Broader source: Energy.gov [DOE]

    The United States, Australia, and Iceland signed a charter on August 28 to launch the International Partnership for Geothermal Technology (IPGT), which will foster and promote cutting-edge geothermal technologies and help address energy security and address global climate change.

  8. Cysteine dioxygenase type 1 promotes adipogenesis via interaction with peroxisome proliferator-activated receptor gamma

    SciTech Connect (OSTI)

    Deng, Peng; Chen, Yi; Ji, Ning; Lin, Yunfeng; Yuan, Quan; Ye, Ling; Chen, Qianming

    2015-02-27

    Mammalian cysteine dioxygenase type 1 (CDO1) is an essential enzyme for taurine biosynthesis and the biodegradation of toxic cysteine. As previously suggested, Cdo1 may be a marker of liposarcoma progression and adipogenic differentiation, but the role of Cdo1 in adipogenesis has yet been reported. In this study, we found that the expression of Cdo1 is dramatically elevated during adipogenic differentiation of 3T3-L1 pre-adipocytes and mouse bone marrow-derived mesenchymal stem cells (mBMSCs). Conversely, knockdown of Cdo1 inhibited expression of adipogenic specific genes and lipid droplet formation in 3T3-L1 cells and mBMSCs. Mechanistically, we found Cdo1 interacted with Pparγ in response to adipogenic stimulus. Further, depletion of Cdo1 reduced the recruitment of Pparγ to the promoters of C/EBPα and Fabp4. Collectively, our finding indicates that Cdo1 may be a co-activator of Pparγ in adipogenesis, and may contribute to the development of disease associated with excessive adipose tissue. - Highlights: • Cdo1expression is highly up-regulated during adipogenic differentiation of 3T3-L1 and mBMSCs. • Depletion of Cdo1 inhibited expression of adipogenic specific genes and lipid droplet formation. • Cdo1interacts with Pparγ during adipogenesis. • Knockdown of Cdo1 inhibited Pparγ binding to the promoters of C/EBPα and Fabp4.

  9. Promoting high efficiency residential HVAC equipment: Lessons learned from leading utility programs

    SciTech Connect (OSTI)

    Neme, C.; Peters, J.; Rouleau, D.

    1998-07-01

    The Consortium for Energy Efficiency recently sponsored a study of leading electric utility efforts to promote high efficiency residential HVAC equipment. Given growing concerns from some utilities about the level of expenditures associated with rebate programs, special emphasis was placed on assessing the success of financing and other non-rebate options for promoting efficiency. Emphasis was also placed on review of efforts--rebate or otherwise--to push the market to very high levels of efficiency (i.e., SEER 13). This paper presents the results of the study. It includes discussion of key lessons from the utility programs analyzed. It also examines program participation rates and other potential indicators of market impacts. One notable conclusion is that several utility programs have pushed market shares for SEER 12 equipment to about 50% (the national average is less than 20%). At least one utility program has achieved a 50% market share for SEER 13 equipment (the national average is less than 3%). In general, financing does not appear to have as broad an appeal as consumer rebates. However, one unique utility program which combines the other of customer financing with modest incentives to contractors--in the form of frequent seller points that can be redeemed for advertising, technician training, travel and other merchandise--offers some promise that high participation rates can be achieved without customer rebates.

  10. Service Academies Research Associates

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Internships » SARA Service Academies Research Associates Military academies come to Los Alamos for a summer of science. October 9, 2015 West Point cadets at the National Security Science Building at Los Alamos National Laboratory. West Point cadets at the National Security Science Building at Los Alamos National Laboratory. Contact Jonathan S. Ventura Email SARA interns use science and engineering to meet challenges to national security. SARA: not what you'd expect Through the Service Academies

  11. National Electrical Manufacturers Association

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    July 24, 2014 VIA EMAIL TO: Regulatory.Review@hq.doe.gov Steven Croley, General Counsel Office of the General Counsel U.S. Department of Energy 1000 Independence Avenue SW., Washington, DC 20585 NEMA Comments on DOE Reducing Regulatory Burden RFI 79 Fed.Reg. 28518 (July 3, 2014) Dear Mr. Croley, The National Electrical Manufacturers Association (NEMA) thanks you for the opportunity to provide comments on the Department of Energy's efforts to make its regulatory program more effective and less

  12. Service Academies Research Associates

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Internships » SARA » SARA Service Academies Research Associates (SARA) The academies come to Los Alamos for a summer of science. Contact Jonathan S. Ventura Email application Program 2015-2016 Application Your Name Date of Birth SSN Home Address Phone Email Address at the Academy Academy Approver's Name Academy Approver's Phone Academy Approver's Email GPA (requires minimum 3.0) Academy Major Foreign Language(s) and Proficiency Preferred Work Environment (lab or office space) Dates Available

  13. Broin Associates | Open Energy Information

    Open Energy Info (EERE)

    search Name: Broin & Associates Place: Sioux Falls, South Dakota Zip: 57104 Product: Bioethanol plant designer, engineering and construction firm References: Broin & Associates1...

  14. Mananook Associates | Open Energy Information

    Open Energy Info (EERE)

    Mananook Associates Jump to: navigation, search Name: Mananook Associates Address: PO Box 69 Place: Perry Zip: 4667 Region: United States Sector: Marine and Hydrokinetic Phone...

  15. Downregulated TIPE2 is associated with poor prognosis and promotes cell proliferation in non-small cell lung cancer

    SciTech Connect (OSTI)

    Li, Yuexia; Li, Xiaohui; Liu, Gang; Sun, Rongqing; Wang, Lirui; Wang, Jing; Wang, Hongmin

    2015-01-30

    Highlights: • TIPE2 is down-regulated in NSCLC tissues. • TIPE2 inhibits NSCLC cell proliferation, colony formation and invasion. • TIPE2 reduces the anti-apoptotic Bcl-XL protein and mesenchymal marker N-cadherin expression. - Abstract: The present study aims to investigate the expression pattern of TIPE2 protein and its clinical significance in human non-small cell lung cancer (NSCLC). We investigated the expression levels of TIPE2 in 96 NSCLC tumor samples by immunohistochemistry and then analyzed its clinical significance. Furthermore, the role of TIPE2 on the biological properties of the NSCLC cell line H1299 and A549 was experimentally tested in vitro and in vivo. We found that the expression level of TIPE2 was significantly higher in normal lung tissues compared with NSCLC tissues (P < 0.001), and TIPE2 downregulation was significantly correlated with advanced TNM stage (P = 0.006). TIPE2 expression was lower in lung cancer cell lines than normal bronchial cell line HBE. Transfection of TIPE2 plasmid was performed in H1299 and A549 cells. TIPE2 overexpression inhibited lung cancer cell proliferation, colony formation and cell invasive in vitro, and prevented lung tumor growth in vivo. In addition, TIPE2 transfection reduced the anti-apoptotic Bcl-XL protein and mesenchymal marker N-cadherin expression. Taken together, our results demonstrate that TIPE2 might serve as a tumor suppressor in NSCLC progression.

  16. NNSA Deputy promotes collaboration on global deterrence at USSTRATCOM

    National Nuclear Security Administration (NNSA)

    deterrence symposium | National Nuclear Security Administration | (NNSA) Deputy promotes collaboration on global deterrence at USSTRATCOM deterrence symposium Wednesday, August 17, 2016 - 2:57pm The first panel of the symposium, focused on ensuring the credibility of nuclear deterrence and assurance capabilities, moderated by Madelyn Creedon. Last month more than 650 experts representing government, military, and academic institutions gathered for the U.S. Strategic Command's (USSTRATCOM)

  17. Switchgrass ubiquitin promoter (PVUBI2) and uses thereof

    DOE Patents [OSTI]

    Stewart, C. Neal; Mann, David George James

    2013-12-10

    The subject application provides polynucleotides, compositions thereof and methods for regulating gene expression in a plant. Polynucleotides disclosed herein comprise novel sequences for a promoter isolated from Panicum virgatum (switchgrass) that initiates transcription of an operably linked nucleotide sequence. Thus, various embodiments of the invention comprise the nucleotide sequence of SEQ ID NO: 2 or fragments thereof comprising nucleotides 1 to 692 of SEQ ID NO: 2 that are capable of driving the expression of an operably linked nucleic acid sequence.

  18. Antonya Sanders-Promoting nanoscience integration through outreach

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Antonya Sanders Antonya Sanders-Promoting nanoscience integration through outreach Bringing together university faculty, students, researchers and other Laboratory scientists to explore nanoscale science. March 17, 2014 Antonya Sanders Sanders' focus on serving others began early on: she lived in England and traveled the world to find ways to help others, including aiding Bosnian refugees. Sanders leads communication and outreach for the Lab's Center for Integrated Nanotechnologies (CINT), a

  19. Material and method for promoting the growth of anaerobic bacteria

    DOE Patents [OSTI]

    Adler, Howard I.

    1984-01-01

    A material and method for promoting the growth of anaerobic bacteria which includes a nutrient media containing a hydrogen donor and sterile membrane fragments of bacteria having an electron transfer system which reduces oxygen to water. Dissolved oxygen in the medium is removed by adding the sterile membrane fragments to the nutrient medium and holding the medium at a temperature of about 10.degree. to about 60.degree. C. until the dissolved oxygen is removed.

  20. Material and method for promoting the growth of anaerobic bacteria

    DOE Patents [OSTI]

    Adler, H.I.

    1984-10-09

    A material and method is disclosed for promoting the growth of anaerobic bacteria which includes a nutrient media containing a hydrogen donor and sterile membrane fragments of bacteria having an electron transfer system which reduces oxygen to water. Dissolved oxygen in the medium is removed by adding the sterile membrane fragments to the nutrient medium and holding the medium at a temperature of about 10 to about 60 C until the dissolved oxygen is removed. No Drawings

  1. Sandia, UNM Form Alliance to Promote Research, Recruiting

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    UNM Form Alliance to Promote Research, Recruiting - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Energy Defense Waste

  2. Structure of a bacterial RNA polymerase holoenzyme open promoter complex

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bae, Brian; Feklistov, Andrey; Lass-Napiorkowska, Agnieszka; Landick, Robert; Darst, Seth A.

    2015-09-08

    Initiation of transcription is a primary means for controlling gene expression. In bacteria, the RNA polymerase (RNAP) holoenzyme binds and unwinds promoter DNA, forming the transcription bubble of the open promoter complex (RPo). We have determined crystal structures, refined to 4.14 Å-resolution, of RPo containing Thermus aquaticus RNAP holoenzyme and promoter DNA that includes the full transcription bubble. The structures, combined with biochemical analyses, reveal key features supporting the formation and maintenance of the double-strand/single-strand DNA junction at the upstream edge of the -10 element where bubble formation initiates. The results also reveal RNAP interactions with duplex DNA just upstreammore » of the -10 element and potential protein/DNA interactions that direct the DNA template strand into the RNAP active site. Additionally a RNA primer to yield a 4 base-pair post-translocated RNA:DNA hybrid mimics an initially transcribing complex at the point where steric clash initiates abortive initiation and σA dissociation.« less

  3. Structure of a bacterial RNA polymerase holoenzyme open promoter complex

    SciTech Connect (OSTI)

    Bae, Brian; Feklistov, Andrey; Lass-Napiorkowska, Agnieszka; Landick, Robert; Darst, Seth A.

    2015-09-08

    Initiation of transcription is a primary means for controlling gene expression. In bacteria, the RNA polymerase (RNAP) holoenzyme binds and unwinds promoter DNA, forming the transcription bubble of the open promoter complex (RPo). We have determined crystal structures, refined to 4.14 Å-resolution, of RPo containing Thermus aquaticus RNAP holoenzyme and promoter DNA that includes the full transcription bubble. The structures, combined with biochemical analyses, reveal key features supporting the formation and maintenance of the double-strand/single-strand DNA junction at the upstream edge of the -10 element where bubble formation initiates. The results also reveal RNAP interactions with duplex DNA just upstream of the -10 element and potential protein/DNA interactions that direct the DNA template strand into the RNAP active site. Additionally a RNA primer to yield a 4 base-pair post-translocated RNA:DNA hybrid mimics an initially transcribing complex at the point where steric clash initiates abortive initiation and σA dissociation.

  4. Oak Ridge Associated

    Office of Legacy Management (LM)

    2012 IL.06 *128 Oak Ridge Associated Post Of/ICE: 80 '17 Unl e Sllles Oa d. )Cp€ T nness £: 37 1 *01 '7 '-1.\0.-»"--" 10. June 14, 1989 Mr. Andrew Wallo ruSRAP/Surplus Facilities Group Division of Facili y & Site Decommissioning Projects Office of Nuclear Energy U.S. D~partment of Energy Washington, D.C. 20545 Subject: LETTER REPORT - VERIFIC~TION ACTIVITIES AT UNIVERSITY OF CHICAGO Dear Mr. Wallo: Enclosed is the report for the recent ORAU verification activities involving

  5. TUNL Associated Theses

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    M.S. and M.A. Degrees Theses Associated Degrees Theses Browse by year 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 2012 Wenzhong Wu, Feedback Systems for Control of Coupled-bunch Instabilities in the Duke Storage Ring. Duke University, May 31, 2012, Supervisor: Y.K. Wu. Download PDF (12 MB) 2011 Botao Jia, Study of Storage Ring Free-Electron Laser Using Experimental and Simulation Approaches. Duke University, July 2011, Supervisor: Y.K. Wu.

  6. associates | National Nuclear Security Administration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    associates Smithsonian Associates Study Tour visits Y-12 The Smithsonian Associates Study Tour. This year marks the second year that the Smithsonian Institution has featured an Oak Ridge tour for its annual Associates Study Tour. Forty-three teachers and two tour leaders traveled from Washington to Oak Ridge for the three-day workshop packed with history...

  7. Northeast Sustainable Energy Association NESEA | Open Energy...

    Open Energy Info (EERE)

    organization focused on promoting the understanding, development, and adoption of energy conservation and non-polluting, renewable energy technologies. Coordinates:...

  8. Promotion of Rural Renewable Energy in Western China | Open Energy...

    Open Energy Info (EERE)

    Energy in Western China Place: Beijing Municipality, China Zip: 100026 Sector: Bioenergy Product: A programme launched by China Association of Rural Energy Industry (CAREI)...

  9. Oak Ridge Associ Universities

    Office of Legacy Management (LM)

    ir.\ "'t-"' , i 'Prepared by Oak Ridge Associ Universities Prepared for Division of Remedial Action Proiects 'U.S. Department of Energy 5 : ! l :;"i\ r l!! ,iri$, t . r ' i , , . 1 . E".:r- i{$, i. 'ii idi 1, . :{. I i:li C O M P R E H E N S I V E R A D I O L O G I C A L S U R V E Y O F F - S I T E P R O P E R T Y W N I A G A R A F A L L S S T O R A G E S I T E LEWlsToN, NEW YORK J . D . B E R G E R Radiol-oglcal Site Assessment Program Manpower Education, Research, and

  10. Oak Ridge Associated

    Office of Legacy Management (LM)

    l/s1 Prepared by Oak Ridge Associated 'Universities Prepared for Division of Remedial Action Froiects ilJ..S. Department of Energy N( , /7 C O M P R E H E N S I V E R A D I O L O G I C A L S U R V E Y O F F . S I T E P R O P E R T Y F N I A G A R A F A L L S S T O R A G E S I T E L E W I S T O N , N E W Y O R K J. D. BERGER Radiologieal Site Assessment Program . Manpower Education, Research, and Training Division FINAL REPORT February 1984 COMPREHENSIVE RADIOLOGICAI SURVEY OFF-SITE PROPERTY F

  11. International District Energy Association

    Broader source: Energy.gov [DOE]

    Since its formation in 1909, the International District Energy Association (IDEA) has served as a principal industry advocate and management resource for owners, operators, developers, and suppliers of district heating and cooling systems in cities, campuses, bases, and healthcare facilities. Today, with over 1,400 members in 26 countries, IDEA continues to organize high-quality technical conferences that inform, connect, and advance the industry toward higher energy efficiency and lower carbon emissions through innovation and investment in scalable sustainable solutions. With the support of DOE, IDEA performs industry research and market analysis to foster high impact projects and help transform the U.S. energy industry. IDEA was an active participant in the original Vision and Roadmap process and has continued to partner with DOE on combined heat and power (CHP) efforts across the country.

  12. Secretary Chu to Travel to India and China to Promote Clean Energy...

    Energy Savers [EERE]

    India and China to Promote Clean Energy Partnerships Secretary Chu to Travel to India and China to Promote Clean Energy Partnerships November 10, 2009 - 12:00am Addthis Washington, ...

  13. Mexico-GTZ Support for the Programme to Promote Solar Water Heating...

    Open Energy Info (EERE)

    Support for the Programme to Promote Solar Water Heating Jump to: navigation, search Logo: Mexico-GTZ Support for the Programme to Promote Solar Water Heating Name Mexico-GTZ...

  14. Establishing and Promoting a Culture of Safety in Chemistry Laboratory Research

    SciTech Connect (OSTI)

    Fryberger, Teresa

    2014-12-23

    Final technical/scientific report for the project, Establishing and Promoting a Culture of Safety in Chemistry Laboratory Research.

  15. Gateways to the FANTOM5 promoter level mammalian expression atlas

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lizio, Marina; Harshbarger, Jayson; Shimoji, Hisashi; Severin, Jessica; Kasukawa, Takeya; Sahin, Serkan; Abugessaisa, Imad; Fukuda, Shiro; Hori, Fumi; Ishikawa-Kato, Sachi; et al

    2015-01-05

    The FANTOM5 project investigates transcription initiation activities in more than 1,000 human and mouse primary cells, cell lines and tissues using CAGE. Based on manual curation of sample information and development of an ontology for sample classification, we assemble the resulting data into a centralized data resource (http://fantom.gsc.riken.jp/5/). In conclusion, this resource contains web-based tools and data-access points for the research community to search and extract data related to samples, genes, promoter activities, transcription factors and enhancers across the FANTOM5 atlas.

  16. E2F1 promote the aggressiveness of human colorectal cancer by activating the ribonucleotide reductase small subunit M2

    SciTech Connect (OSTI)

    Fang, Zejun; Gong, Chaoju; Liu, Hong; Zhang, Xiaomin; Mei, Lingming; Song, Mintao; Qiu, Lanlan; Luo, Shuchai; Zhu, Zhihua; Zhang, Ronghui; Gu, Hongqian; Chen, Xiang

    2015-08-21

    As the ribonucleotide reductase small subunit, the high expression of ribonucleotide reductase small subunit M2 (RRM2) induces cancer and contributes to tumor growth and invasion. In several colorectal cancer (CRC) cell lines, we found that the expression levels of RRM2 were closely related to the transcription factor E2F1. Mechanistic studies were conducted to determine the molecular basis. Ectopic overexpression of E2F1 promoted RRM2 transactivation while knockdown of E2F1 reduced the levels of RRM2 mRNA and protein. To further investigate the roles of RRM2 which was activated by E2F1 in CRC, CCK-8 assay and EdU incorporation assay were performed. Overexpression of E2F1 promoted cell proliferation in CRC cells, which was blocked by RRM2 knockdown attenuation. In the migration and invasion tests, overexpression of E2F1 enhanced the migration and invasion of CRC cells which was abrogated by silencing RRM2. Besides, overexpression of RRM2 reversed the effects of E2F1 knockdown partially in CRC cells. Examination of clinical CRC specimens demonstrated that both RRM2 and E2F1 were elevated in most cancer tissues compared to the paired normal tissues. Further analysis showed that the protein expression levels of E2F1 and RRM2 were parallel with each other and positively correlated with lymph node metastasis (LNM), TNM stage and distant metastasis. Consistently, the patients with low E2F1 and RRM2 levels have a better prognosis than those with high levels. Therefore, we suggest that E2F1 can promote CRC proliferation, migration, invasion and metastasis by regulating RRM2 transactivation. Understanding the role of E2F1 in activating RRM2 transcription will help to explain the relationship between E2F1 and RRM2 in CRC and provide a novel predictive marker for diagnosis and prognosis of the disease. - Highlights: • E2F1 promotes RRM2 transactivation in CRC cells. • E2F1 promotes the proliferation of CRC cells by activating RRM2. • E2F1 promotes the migration and

  17. Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression

    DOE Patents [OSTI]

    Gao, Johnway [Richland, WA; Skeen, Rodney S [Pendleton, OR; Hooker, Brian S [Kennewick, WA; Anderson, Daniel B [Pasco, WA

    2003-04-01

    The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

  18. The soil microbiome influences grapevine-associated microbiota

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zarraonaindia, Iratxe; Owens, Sarah M.; Weisenhorn, Pamela; West, Kristin; Hampton-Marcell, Jarrad; Lax, Simon; Bokulich, Nicholas A.; Mills, David A.; Martin, Gilles; Taghavi, Safiyh; et al

    2015-03-24

    Grapevine is a well-studied, economically relevant crop, whose associated bacteria could influence its organoleptic properties. In this study, the spatial and temporal dynamics of the bacterial communities associated with grapevine organs (leaves, flowers, grapes, and roots) and soils were characterized over two growing seasons to determine the influence of vine cultivar, edaphic parameters, vine developmental stage (dormancy, flowering, preharvest), and vineyard. Belowground bacterial communities differed significantly from those aboveground, and yet the communities associated with leaves, flowers, and grapes shared a greater proportion of taxa with soil communities than with each other, suggesting that soil may serve as a bacterialmore » reservoir. A subset of soil microorganisms, including root colonizers significantly enriched in plant growth-promoting bacteria and related functional genes, were selected by the grapevine. In addition to plant selective pressure, the structure of soil and root microbiota was significantly influenced by soil pH and C:N ratio, and changes in leaf- and grape-associated microbiota were correlated with soil carbon and showed interannual variation even at small spatial scales. Diazotrophic bacteria, e.g., Rhizobiaceae and Bradyrhizobium spp., were significantly more abundant in soil samples and root samples of specific vineyards. Vine-associated microbial assemblages were influenced by myriad factors that shape their composition and structure, but the majority of organ-associated taxa originated in the soil, and their distribution reflected the influence of highly localized biogeographic factors and vineyard management.« less

  19. The soil microbiome influences grapevine-associated microbiota

    SciTech Connect (OSTI)

    Zarraonaindia, Iratxe; Owens, Sarah M.; Weisenhorn, Pamela; West, Kristin; Hampton-Marcell, Jarrad; Lax, Simon; Bokulich, Nicholas A.; Mills, David A.; Martin, Gilles; Taghavi, Safiyh; van der Lelie, Daniel; Gilbert, Jack A.

    2015-03-24

    Grapevine is a well-studied, economically relevant crop, whose associated bacteria could influence its organoleptic properties. In this study, the spatial and temporal dynamics of the bacterial communities associated with grapevine organs (leaves, flowers, grapes, and roots) and soils were characterized over two growing seasons to determine the influence of vine cultivar, edaphic parameters, vine developmental stage (dormancy, flowering, preharvest), and vineyard. Belowground bacterial communities differed significantly from those aboveground, and yet the communities associated with leaves, flowers, and grapes shared a greater proportion of taxa with soil communities than with each other, suggesting that soil may serve as a bacterial reservoir. A subset of soil microorganisms, including root colonizers significantly enriched in plant growth-promoting bacteria and related functional genes, were selected by the grapevine. In addition to plant selective pressure, the structure of soil and root microbiota was significantly influenced by soil pH and C:N ratio, and changes in leaf- and grape-associated microbiota were correlated with soil carbon and showed interannual variation even at small spatial scales. Diazotrophic bacteria, e.g., Rhizobiaceae and Bradyrhizobium spp., were significantly more abundant in soil samples and root samples of specific vineyards. Vine-associated microbial assemblages were influenced by myriad factors that shape their composition and structure, but the majority of organ-associated taxa originated in the soil, and their distribution reflected the influence of highly localized biogeographic factors and vineyard management.

  20. Environmental Management Associates | Open Energy Information

    Open Energy Info (EERE)

    Environmental Management Associates (Redirected from EMA Associates) Jump to: navigation, search Name: Environmental Management Associates Abbreviation: EMA Website:...

  1. Strategies for promoting renewables in a new electric industry

    SciTech Connect (OSTI)

    Driver, B.

    1996-12-31

    This paper describes strategies for promoting renewable resources in an era characterized by competitive pressures in the electric industry. It begins with a background section to describe the perspective from which I am writing and the nature of the pressures confronting renewables in 1996. Then, the paper turns to a discussion of the regulatory and other options to promote renewables in this environment. The major conclusion of the paper is that there is no {open_quotes}magic bullet{close_quotes} to guide the development of renewables through the developing competitive era within the electric industry. Indeed, it appears that the job can get done only through a combination of different measures at all levels of government. The author believes that among the most effective measures are likely to be: a national renewable resources generation standard; conditions attached to restructuring events; regional interstate compacts; regional risk-sharing consortia supported by federal and state tax and fiscal policy; and state {open_quotes}systems benefits charges;{close_quotes}

  2. Low severity coal liquefaction promoted by cyclic olefins

    SciTech Connect (OSTI)

    Curtis, C.W.

    1992-07-27

    Low severity coal liquefaction allows for solubilization of coal with reduced gas make. These lower severity conditions may result in some selective bond rupture. Promotion of coal solubilization through hydrogen transfer using highly active and effective hydrogen donors is the objective of this study. The highly effective donors being tested are cyclic olefins. Representative cyclic olefins are isotetralin, which is 1,4,5,8-tetrahydronaphthalene, and 1,4,5,8,9,10-hexahydroanthracene. These compounds are hydroaromatics without aromatic rings and have been shown to be highly effective donors. The objective of the work performed in this study during this quarter was to evaluate reaction parameters for low severity liquefaction reactions using the cyclic olefin, hexahydroanthracene, and the aromatic, anthracene. These model compounds were reacted under a variety of conditions to evaluate their reactivity without coal. The reactions were performed under both thermal and catalytic conditions. Finely divided catalysts from different molybdenum precursors were used to determine their activity in promoting hydrogenation and hydrogen transfer at low severity conditions. The catalysts used were Molyvan L, sulfurized oxymolybdenum dithiocarbamate, molybdenum naphthenate, and Molyvan 822, organo molybdenum dithiocarbamate.

  3. Activation of type 2 cannabinoid receptors (CB2R) promotes fatty acid oxidation through the SIRT1/PGC-1? pathway

    SciTech Connect (OSTI)

    Zheng, Xuqin; Sun, Tao; Wang, Xiaodong

    2013-07-05

    Highlights: TC, a CB2R specific agonist, stimulates SIRT1 activity by PKA/CREB pathway. TC promotes PGC-1? transcriptional activity by increasing its deacetylation. TC increases the expression of genes linked to FAO and promotes the rate of FAO. The effects of TC in FAO are dependent on CB2R. Suggesting CB2R as a target to treat diseases with lipid dysregulation. -- Abstract: Abnormal fatty acid oxidation has been associated with obesity and type 2 diabetes. At the transcriptional level, peroxisome proliferator-activated receptor-gamma coactivator 1? (PGC-1?) has been reported to strongly increase the ability of hormone nuclear receptors PPAR? and ERR? to drive transcription of fatty acid oxidation enzymes. In this study, we report that a specific agonist of the type 2 cannabinoid receptor (CB2R) can lead to fatty acid oxidation through the PGC-1? pathway. We have found that CB2R is expressed in differentiated C2C12 myotubes, and that use of the specific agonist trans-caryophyllene (TC) stimulates sirtuin 1 (SIRT1) deacetylase activity by increasing the phosphorylation of cAMP response element-binding protein (CREB), thus leading to increased levels of PGC-1? deacetylation. This use of TC treatment increases the expression of genes linked to the fatty acid oxidation pathway in a SIRT1/PGC-1?-dependent mechanism and also drastically accelerates the rate of complete fatty acid oxidation in C2C12 myotubes, neither of which occur when CB2R mRNA is knocked down using siRNA. These results reveal that activation of CB2R by a selective agonist promotes lipid oxidation through a signaling/transcriptional pathway. Our findings imply that pharmacological manipulation of CB2R may provide therapeutic possibilities to treat metabolic diseases associated with lipid dysregulation.

  4. A neural network system for prediction of RNA polymerase II promoters

    SciTech Connect (OSTI)

    Matis, S.; Shah, M.; Mural, R.; Uberbacher, E.

    1994-12-31

    One of the most difficult problems in the analysis of eucaryotic genes is the detection of RNA polymerase II promoter regions. Although promoter regions vary in the primary DNA sequence, a basic group of core promoter elements has been suggested in the literature. Many human promoter sequences contain a TATAA sequence element at approximately 30 bases upstream of the cap site (transcription start site). Other elements are the GC box which binds SPA and upregulates transcription, the CAAT box, and the ATG initiator codon. To characterize promoters, we constructed frequency matrices for each element using experimentally mapped human promoter regions. Additionally, we constructed histograms for the distances separating the various elements. We then used a neural network to combine these informational elements. The output of the neural network is then processed using a set of expert rules which depend on GRAIL`s ability to find exons in anonymous DNA. This improves the selectivity of promoter detection and reduces the false positive rate.

  5. Encapsulant materials and associated devices

    DOE Patents [OSTI]

    Kempe, Michael D; Thapa, Prem

    2012-05-22

    Compositions suitable for use as encapsulants are described. The inventive compositions include a high molecular weight polymeric material, a curing agent, an inorganic compound, and a coupling agent. Optional elements include adhesion promoting agents, colorants, antioxidants, and UV absorbers. The compositions have desirable diffusivity properties, making them suitable for use in devices in which a substantial blocking of moisture ingress is desired, such as photovoltaic (PV) modules.

  6. Encapsulant materials and associated devices

    DOE Patents [OSTI]

    Kempe, Michael D; Thapa, Prem

    2011-03-08

    Compositions suitable for use as encapsulants are described. The inventive compositions include a high molecular weight polymeric material, a curing agent, an inorganic compound, and a coupling agent. Optional elements include adhesion promoting agents, colorants, antioxidants, and UV absorbers. The compositions have desirable diffusivity properties, making them suitable for use in devices in which a substantial blocking of moisture ingress is desired, such as photovoltaic (PV) modules.

  7. Harmine promotes osteoblast differentiation through bone morphogenetic protein signaling

    SciTech Connect (OSTI)

    Yonezawa, Takayuki; Lee, Ji-Won; Hibino, Ayaka; Asai, Midori; Hojo, Hironori; Cha, Byung-Yoon; Teruya, Toshiaki; Nagai, Kazuo; Chung, Ung-Il; Yagasaki, Kazumi; and others

    2011-06-03

    Highlights: {yields} Harmine promotes the activity and mRNA expression of ALP. {yields} Harmine enhances the expressions of osteocalcin mRNA and protein. {yields} Harmine induces osteoblastic mineralization. {yields} Harmine upregulates the mRNA expressions of BMPs, Runx2 and Osterix. {yields} BMP signaling pathways are involved in the actions of harmine. -- Abstract: Bone mass is regulated by osteoblast-mediated bone formation and osteoclast-mediated bone resorption. We previously reported that harmine, a {beta}-carboline alkaloid, inhibits osteoclast differentiation and bone resorption in vitro and in vivo. In this study, we investigated the effects of harmine on osteoblast proliferation, differentiation and mineralization. Harmine promoted alkaline phosphatase (ALP) activity in MC3T3-E1 cells without affecting their proliferation. Harmine also increased the mRNA expressions of the osteoblast marker genes ALP and Osteocalcin. Furthermore, the mineralization of MC3T3-E1 cells was enhanced by treatment with harmine. Harmine also induced osteoblast differentiation in primary calvarial osteoblasts and mesenchymal stem cell line C3H10T1/2 cells. Structure-activity relationship studies using harmine-related {beta}-carboline alkaloids revealed that the C3-C4 double bond and 7-hydroxy or 7-methoxy group of harmine were important for its osteogenic activity. The bone morphogenetic protein (BMP) antagonist noggin and its receptor kinase inhibitors dorsomorphin and LDN-193189 attenuated harmine-promoted ALP activity. In addition, harmine increased the mRNA expressions of Bmp-2, Bmp-4, Bmp-6, Bmp-7 and its target gene Id1. Harmine also enhanced the mRNA expressions of Runx2 and Osterix, which are key transcription factors in osteoblast differentiation. Furthermore, BMP-responsive and Runx2-responsive reporters were activated by harmine treatment. Taken together, these results indicate that harmine enhances osteoblast differentiation probably by inducing the expressions of

  8. 1 Outreach, Education and Domestic Market Enhancement 2 Export Promotion and Assistance

    SciTech Connect (OSTI)

    Geothermal Energy Association

    2004-03-15

    Geothermal Energy Association supports the US geothermal industry in its efforts to bring more clean geothermal energy on-line throughout the world. Activities designed to accomplish this goal include: (1) developing and maintaining data bases, web pages, (2) commissioning of special studies and reports, (3) preparing, printing and distributing brochures and newsletters, (4) developing exhibits and displays, and participating in trade shows, (5) designing, producing and disseminating audio-video materials, (6) monitoring and coordinating programs carried out by US DOE and other Federal agencies, (7) holding workshops to facilitate communication between researchers and industry and to encourage their recognition of emerging markets for geothermal technology, (8) attending conferences, making speeches and presentation, and otherwise interacting with environmental and other renewable energy organizations and coalitions, (9) hosting events in Washington, DC and other appropriate locations to educate Federal, State and local representatives, environmental groups, the news media, and other about the status and potential of geothermal energy, (10) conducting member services such as the preparation and distribution of a member newsletter related to operating and maintaining s useful and viable association, and (11) performing similar kinds of activities designed to inform others about geothermal energy. The activities of the export promotion aim to assist industry in accomplishing the goal of successfully penetrating and developing energy in country with existing geothermal resources and a desire to develop them. Activities including in export promotion are: (1)needs analysis and assessment involve monitoring the progress of developing markets and projects overseas and working with US industry to determine what future activities by GEA would be of greatest assistance, (2) outreach includes the preparation and dissemination of brochures and videos for foreign professionals

  9. Promoting Behavior-Based Energy Efficiency in Military Housing

    SciTech Connect (OSTI)

    AH McMakin; EL Malone; RE Lundgren

    1999-09-07

    The U.S. Department of Energy's Federal Energy Management Program (FEMP) helps agencies reduce the cost of doing business through energy efficiency, water conservation, and the use of solar and other renewable energy. As a large energy user, the U.S. military has been one of the government sectors of focus. Several military installations have shown substantial energy savings in past years. Most of these efficiency projects, however, have focused primarily on physical upgrades, technologies, and purchasing habits. Furthermost projects have focused on administrative and operational areas of energy use. Military residential housing, in particular, has received little formal attention for energy efficiency involving behaviors of the residents themselves. Behavior-based change is a challenging, but potentially fruitful area for energy conservation programs. However, behavioral change involves links with values, social networks and organizations, and new ways of thinking about living patterns. This handbook attempts to fill a gap by offering guidance for promoting such efforts.

  10. Selection against spurious promoter motifs correlates withtranslational efficiency across bacteria

    SciTech Connect (OSTI)

    Froula, Jeffrey L.; Francino, M. Pilar

    2007-05-01

    Because binding of RNAP to misplaced sites could compromise the efficiency of transcription, natural selection for the optimization of gene expression should regulate the distribution of DNA motifs capable of RNAP-binding across the genome. Here we analyze the distribution of the -10 promoter motifs that bind the {sigma}{sup 70} subunit of RNAP in 42 bacterial genomes. We show that selection on these motifs operates across the genome, maintaining an over-representation of -10 motifs in regulatory sequences while eliminating them from the nonfunctional and, in most cases, from the protein coding regions. In some genomes, however, -10 sites are over-represented in the coding sequences; these sites could induce pauses effecting regulatory roles throughout the length of a transcriptional unit. For nonfunctional sequences, the extent of motif under-representation varies across genomes in a manner that broadly correlates with the number of tRNA genes, a good indicator of translational speed and growth rate. This suggests that minimizing the time invested in gene transcription is an important selective pressure against spurious binding. However, selection against spurious binding is detectable in the reduced genomes of host-restricted bacteria that grow at slow rates, indicating that components of efficiency other than speed may also be important. Minimizing the number of RNAP molecules per cell required for transcription, and the corresponding energetic expense, may be most relevant in slow growers. These results indicate that genome-level properties affecting the efficiency of transcription and translation can respond in an integrated manner to optimize gene expression. The detection of selection against promoter motifs in nonfunctional regions also implies that no sequence may evolve free of selective constraints, at least in the relatively small and unstructured genomes of bacteria.

  11. Chinese Renewable Energy Industries Association CREIA | Open...

    Open Energy Info (EERE)

    Product: CREIA promotes the adoption of advanced technologies among renewable energy enterprises in China and actively develops capacity for the rapid industrialisation of the...

  12. Weapons Program Associate Directors named

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    integration we have achieved between the various components of the program," said Bret Knapp, Principal Associate Director for Weapons Programs. "They have both done an...

  13. Principal Associate Director - Weapons Programs

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Weapons Programs As Principal Associate Director for the Weapons Program, Robert Webster leads the programs to assure the safety, security, and effectiveness of the systems in the...

  14. Principal Associate Director - Capital Projects

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    As Principal Associate Director for Capital Projects (interim), Larry Simmons is responsible for institutional large-project construction and management and environmental cleanup ...

  15. Stanniocalcin-2 is a HIF-1 target gene that promotes cell proliferation in hypoxia

    SciTech Connect (OSTI)

    Law, Alice Y.S.; Wong, Chris K.C.

    2010-02-01

    Stanniocalcin-2 (STC2), the paralog of STC1, has been suggested as a novel target of oxidative stress response to protect cells from apoptosis. The expression of STC2 has been reported to be highly correlated with human cancer development. In this study, we reported that STC2 is a HIF-1 target gene and is involved in the regulation of cell proliferation. STC2 was shown to be up-regulated in different breast and ovarian cancer cells, following exposure to hypoxia. Using ovarian cancer cells (SKOV3), the underlying mechanism of HIF-1 mediated STC2 gene transactivation was characterized. Hypoxia-induced STC2 expression was found to be HIF-1{alpha} dependent and required the recruitment of p300 and HDAC7. Using STC2 promoter deletion constructs and site-directed mutagenesis, two authentic consensus HIF-1 binding sites were identified. Under hypoxic condition, the silencing of STC2 reduced while the overexpression of STC2 increased the levels of phosphorylated retinoblastoma and cyclin D in both SKOV3 and MCF7 cells. The change in cell cycle proteins correlated with the data of the serial cell counts. The results indicated that cell proliferation was reduced in STC2-silenced cells but was increased in STC2-overexpressing hypoxic cells. Solid tumor progression is usually associated with hypoxia. The identification and functional analysis of STC2 up-regulation by hypoxia, a feature of the tumor microenvironment, sheds light on a possible role for STC2 in tumors.

  16. MicroRNA-196b promotes cell proliferation and suppress cell differentiation in vitro

    SciTech Connect (OSTI)

    Cao, Donglin Hu, Liangshan; Lei, Da; Fang, Xiaolin; Zhang, Zhihong; Wang, Ting; Lin, Maorui; Huang, Jiwei; Yang, Huawen; Zhou, Xuan; Zhong, Limei

    2015-01-30

    Highlights: • miRNA-196b increases proliferation and blocks differentiation of progenitor cell. • miRNA-196b inhibits apoptosis and increases viability of cells lines. • Forced expression of miR-196b blocks the differentiation of THP1 induced by PMA. - Abstract: MicroRNA-196b (miR-196b) is frequently amplified and aberrantly overexpressed in acute leukemias. To investigate the role of miR-196b in acute leukemias, it has been observed that forced expression of this miRNA increases proliferation and inhibits apoptosis in human cell lines. More importantly, we show that this miRNA can significantly increase the colony-forming capacity of mouse normal bone marrow progenitor cells alone, as well as partially blocking the cells from differentiation. Taken together, our studies suggest that miRNA-196b may play an essential role in the development of MLL-associated leukemias through inhibiting cell differentiation and apoptosis, while promoting cell proliferation.

  17. Promoting Uranium Immobilization by the Activities of Microbial Phosphatases

    SciTech Connect (OSTI)

    Robert J. Martinez; Melanie J. Beazley; Samuel M. Webb; Martial Taillefert; and Patricia A. Sobecky

    2007-04-19

    The overall objective of this project is to examine the activity of nonspecific phosphohydrolases present in naturally occurring subsurface microorganisms for the purpose of promoting the immobilization of radionuclides through the production of uranium [U(VI)] phosphate precipitates. Specifically, we hypothesize that the precipitation of U(VI) phosphate minerals may be promoted through the microbial release and/or accumulation of PO4 3- as a means to detoxify radionuclides and heavy metals. An experimental approach was designed to determine the extent of phosphatase activity in bacteria previously isolated from contaminated subsurface soils collected at the ERSP Field Research Center (FRC) in Oak Ridge, TN. Screening of 135 metal resistant isolates for phosphatase activity indicated the majority (75 of 135) exhibited a phosphatase-positive phenotype. During this phase of the project, a PCR based approach has also been designed to assay FRC isolates for the presence of one or more classes of the characterized non-specific acid phophastase (NSAP) genes likely to be involved in promoting U(VI) precipitation. Testing of a subset of Pb resistant (Pbr) Arthrobacter, Bacillus and Rahnella strains indicated 4 of the 9 Pbr isolates exhibited phosphatase phenotypes suggestive of the ability to bioprecipitate U(VI). Two FRC strains, a Rahnella sp. strain Y9602 and a Bacillus sp. strain Y9-2, were further characterized. The Rahnella sp. exhibited enhanced phosphatase activity relative to the Bacillus sp. Whole-cell enzyme assays identified a pH optimum of 5.5, and inorganic phosphate accumulated in pH 5.5 synthetic groundwater (designed to mimic FRC conditions) incubations of both strains in the presence of a model organophosphorus substrate provided as the sole C and P source. Kinetic experiments showed that these two organisms can grow in the presence of 200 μM dissolved uranium and that Rahnella is much more efficient in precipitating U(VI) than Bacillus sp. The

  18. Chemical inducible promoter used to obtain transgenic plants with a silent marker

    DOE Patents [OSTI]

    Aoyama, Takashi; Zuo, Jianru; Chua, Nam-Hai

    2004-08-31

    A chemically inducible promoter is described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoter described is one that is inducible by a glucocorticoid which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt or knotted1 to induce shoot formation in the presence of a glucocorticoid. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.

  19. Energy Secretary Bodman Heads to West Virginia to Promote Energy Bill |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Heads to West Virginia to Promote Energy Bill Energy Secretary Bodman Heads to West Virginia to Promote Energy Bill July 7, 2005 - 2:00pm Addthis Secretary Promotes Energizing America for Energy Security BELLE, WV - Secretary of Energy Samuel W. Bodman today traveled to West Virginia to urge the Congress to pass comprehensive energy legislation that is now before them. The bill reflects many of the principles of President Bush's national energy policy including the

  20. Secretary Chu Announces New Efforts to Promote Clean Energy in Tribal

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Communities | Department of Energy Efforts to Promote Clean Energy in Tribal Communities Secretary Chu Announces New Efforts to Promote Clean Energy in Tribal Communities January 19, 2011 - 12:00am Addthis WASHINGTON - U.S. Energy Secretary Steven Chu announced today two new initiatives to promote tribal energy development and continue strengthening the partnership between the Department of Energy and tribal nations. Up to $10 million will be available this year through DOE's Tribal Energy

  1. DOE Secretary Promotes E85 Use in Indianapolis | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Secretary Promotes E85 Use in Indianapolis DOE Secretary Promotes E85 Use in Indianapolis May 11, 2006 - 10:40am Addthis INDIANAPOLIS , IN - Department of Energy Secretary Samuel W. Bodman today praised and promoted the expanded use and availability of ethanol-based fuel during two events in Indianapolis. Expanding the use of ethanol, specifically cellulosic ethanol, is a key component of President Bush's Advanced Energy Initiative (AEI), which seeks to reduce our reliance on foreign oil by 5

  2. Executive Order 13583, Establishing a Coordinated Government-Wide Initiative to Promote Diversity and Inclusion in the Federal Workforce

    Broader source: Energy.gov [DOE]

    Establishes a "coordinated government-wide initiative to promote diversity and inclusion in the federal workforce".

  3. Energy Portfolio Standards and the Promotion of Combined Heat and Power (CHP) White Paper, April 2009

    Office of Energy Efficiency and Renewable Energy (EERE)

    EPA CHP Partnership’s white paper provides information on energy portfolio standards and how they promote combined heat and power.

  4. A New Scheme for the Promotion of Renewable Energies in Developing...

    Open Energy Info (EERE)

    Regulated Purchase Tariff Jump to: navigation, search Tool Summary LAUNCH TOOL Name: A New Scheme for the Promotion of Renewable Energies in Developing Countries: The Renewable...

  5. Processes and palladium-promoted catalysts for conducting Fischer-Tropsch synthesis

    DOE Patents [OSTI]

    Singleton, Alan H.; Oukaci, Rachid; Goodwin, James G.

    2000-01-01

    A process for hydrocarbon synthesis comprising the step of reacting a synthesis gas in the presence of a cobalt catalyst promoted with palladium.

  6. PEGylation of a Maltose Biosensor Promotes Enhanced Signal Response

    SciTech Connect (OSTI)

    Dattelbaum, Andrew; Baker, Gary A; Fox, John M; Iyer, Srinivas; Dattelbaum, Jonathan

    2009-01-01

    A robust method to immobilize a maltose biosensor is described using an engineered maltose periplasmic binding protein (PBP) covalently coupled to NBDamide, an environmentally sensitive fluorophore. A mesoporous silica sol-gel derived from diglycerylsilane (DGS) was constructed to embed the maltose biosensor, and the ligand reporting fluorescence properties were meas red. When sequestered in the DGS-derived silica matrix, the biosensor retained maltose-dependent fluorescence sensing capability with micromolar affinity, which is consistent with the protein free in solution. The MBP-NBD conjugate was further modified by covalent conjugation with poly(ethylene glycol)-5000 (PEG) to promote the retention of water molecules around the protein and to reduce possible steric effects between the silica matrix and protein. Bioconjugation with PEG molecules does not significantly affect the signaling response of the protein in solution. When immobilized in the DGS polymer, a consistent increase in fluorescence intensity was observed as compared to the protein not functionalized with PEG. To our knowledge, this report presents the first successful method to embed a PBP biosensor in a polymerized matrix and retain signaling response using an environmentally sensitive probe. The immobilization method presented here should be easily adaptable to all conformation-dependent biosensors.

  7. The Los Alamos universe: Using multimedia to promote laboratory capabilities

    SciTech Connect (OSTI)

    Kindel, J.

    2000-03-01

    This project consists of a multimedia presentation that explains the technological capabilities of Los Alamos National Laboratory. It takes the form of a human-computer interface built around the metaphor of the universe. The project is intended promote Laboratory capabilities to a wide audience. Multimedia is simply a means of communicating information through a diverse set of tools--be they text, sound, animation, video, etc. Likewise, Los Alamos National Laboratory is a collection of diverse technologies, projects, and people. Given the ample material available at the Laboratory, there are tangible benefits to be gained by communicating across media. This paper consists of three parts. The first section provides some basic information about the Laboratory, its mission, and its needs. The second section introduces this multimedia presentation and the metaphor it is based on along with some basic concepts of color and user interaction used in the building of this project. The final section covers construction of the project, pitfalls, and future improvements.

  8. Research Affiliate Associates & Students | Photosynthetic Antenna...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Affiliate Associates & Students Research Affiliate Associates & Students Michal Gwizdala Postdoctoral Research Associate E-mail: m.s.gwizdala@vu.nl Atsuko Kanazawa Research...

  9. Kodiak Electric Association KEA | Open Energy Information

    Open Energy Info (EERE)

    Electric Association KEA Jump to: navigation, search Name: Kodiak Electric Association (KEA) Place: Kodiak, Alaska Zip: 99614 Product: Kodiak Electric Association, Inc. (KEA) is a...

  10. Electric Drive Transportation Association EDTA | Open Energy...

    Open Energy Info (EERE)

    Transportation Association EDTA Jump to: navigation, search Name: Electric Drive Transportation Association (EDTA) Product: EDTA is the preeminent U.S. industry association...

  11. International Hydropower Association | Open Energy Information

    Open Energy Info (EERE)

    International Hydropower Association Place: United Kingdom Zip: SM1 4JH Sector: Hydro Product: The International Hydropower Association is a non-governmental mutual association of...

  12. British Hydropower Association | Open Energy Information

    Open Energy Info (EERE)

    British Hydropower Association Place: Wimborne, Dorset, United Kingdom Zip: BH21 1QU Sector: Hydro Product: The British Hydropower Association (BHA) is a trade association which...

  13. World Wind Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Association Jump to: navigation, search Logo: World Wind Energy Association Name: World Wind Energy Association Address: Charles-de-Gaulle-Str. 5 Place: Bonn, Germany Phone Number:...

  14. European Wind Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Energy Association Jump to: navigation, search Logo: European Wind Energy Association Name: European Wind Energy Association Address: Rue d'Arlon 80 B-1040 Place: Brussels, Belgium...

  15. Northeast Sustainable Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Association Jump to: navigation, search Logo: Northeast Sustainable Energy Association Name: Northeast Sustainable Energy Association Address: 50 Miles Street Place: Greenfield,...

  16. German Biogas Association | Open Energy Information

    Open Energy Info (EERE)

    Association Jump to: navigation, search Name: German Biogas Association Place: Freising, Germany Zip: 85356 Product: The Association represents the interests of biogas plant...

  17. Geothermal Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Association (Redirected from GEA) Jump to: navigation, search Logo: Geothermal Energy Association Name: Geothermal Energy Association Address: 209 Pennsylvania Avenue SE Place:...

  18. Geothermal Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Association Jump to: navigation, search Logo: Geothermal Energy Association Name: Geothermal Energy Association Address: 209 Pennsylvania Avenue SE Place: Washington, DC Zip: 20003...

  19. Association

    National Nuclear Security Administration (NNSA)

    ......... 7 ' 3.0 Accident Rates ......13 4. Frequencies of Accident Types (TIFA 1980-1989, tractor ...

  20. Association

    National Nuclear Security Administration (NNSA)

    This report describes the development of accident rate influence factors as related to the ......... 7 ' 3.0 Accident Rates ......

  1. Association

    Office of Scientific and Technical Information (OSTI)

    ... is well known to exhibit16 I i quite intersting phenomena such as hysteresis and chaos. ... Lett. 23, 142 (1973). 8 H. Ikezi, J. S. DeGrassie and J. Drake, "Soliton generation at1...

  2. Valley Electric Association- Net Metering

    Broader source: Energy.gov [DOE]

    The Board of Directors for Valley Electric Association (VEA) approved net metering in April 2008. The rules apply to systems up to 30 kW, though owners of larger systems may be able to negotiate...

  3. Principal Associate Director - Global Security

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Principal Associate Director - Global Security As Principal Associate Director for Global Security, Terry Wallace leads Laboratory programs with special focus on developing and applying the scientific and engineering capabilities to address complex national and global security threats. Contact Operator Los Alamos National Laboratory (505) 667-5061 Wallace's expertise is forensic seismology, a highly specialized discipline focusing on detection and quantification of nuclear tests. Terry C.

  4. Promoting system-level learning from project-level lessons

    SciTech Connect (OSTI)

    Jong, Amos A. de; Runhaar, Hens A.C.; Runhaar, Piety R.; Kolhoff, Arend J.; Driessen, Peter P.J.

    2012-02-15

    A growing number of low and middle income nations (LMCs) have adopted some sort of system for environmental impact assessment (EIA). However, generally many of these EIA systems are characterised by a low performance in terms of timely information dissemination, monitoring and enforcement after licencing. Donor actors (such as the World Bank) have attempted to contribute to a higher performance of EIA systems in LMCs by intervening at two levels: the project level (e.g. by providing scoping advice or EIS quality review) and the system level (e.g. by advising on EIA legislation or by capacity building). The aims of these interventions are environmental protection in concrete cases and enforcing the institutionalisation of environmental protection, respectively. Learning by actors involved is an important condition for realising these aims. A relatively underexplored form of learning concerns learning at EIA system-level via project level donor interventions. This 'indirect' learning potentially results in system changes that better fit the specific context(s) and hence contribute to higher performances. Our exploratory research in Ghana and the Maldives shows that thus far, 'indirect' learning only occurs incidentally and that donors play a modest role in promoting it. Barriers to indirect learning are related to the institutional context rather than to individual characteristics. Moreover, 'indirect' learning seems to flourish best in large projects where donors achieved a position of influence that they can use to evoke reflection upon system malfunctions. In order to enhance learning at all levels donors should thereby present the outcomes of the intervention elaborately (i.e. discuss the outcomes with a large audience), include practical suggestions about post-EIS activities such as monitoring procedures and enforcement options and stimulate the use of their advisory reports to generate organisational memory and ensure a better information dissemination.

  5. Low-temperature superacid catalysis: Reactions of n-butane catalyzed by iron- and manganese-promoted sulfated zirconia

    SciTech Connect (OSTI)

    Cheung, T.K.; D`Itri, J.L.; Gates, B.C.

    1995-02-01

    Environmental concerns are leading to the replacement of aromatic hydrocarbons in gasoline with high-octane-number branched paraffins and oxygenated compounds such as methyl t-butyl ether, which is produced from methanol and isobutylene. The latter can be formed from n-butane by isomerization followed by dehydrogenation. To meet the need for improved catalysts for isomerization of n-butane and other paraffins, researchers identified solid acids that are noncorrosive and active at low temperatures. Sulfated zirconia catalyzes the isomerization of n-butane even at 25{degrees}C, and the addition of Fe and Mn promoters increases its activity by three orders of magnitude. Little is known about this new catalyst. Here the authors provide evidence of its performance for n-butane conversion, demonstrating that isomerization is accompanied by disproportionation and other, less well understood, acid-catalyzed reactions and undergoes rapid deactivation associated with deposition of carbonaceous material. 10 refs., 3 figs.

  6. Voluntary Protection Programs Participants' Association (VPPPA...

    Office of Environmental Management (EM)

    Programs Participants' Association (VPPPA) Presentation: Conducting your Annual VPP Self Assessment 2009 Voluntary Protection Programs Participants' Association (VPPPA)...

  7. 2009 Voluntary Protection Programs Participants' Association...

    Office of Environmental Management (EM)

    Programs Participants' Association (VPPPA) Presentation: Conducting your Annual VPP Self Assessment 2009 Voluntary Protection Programs Participants' Association (VPPPA)...

  8. VDE Association for Electrical Electronic Information Technologies...

    Open Energy Info (EERE)

    VDE Association for Electrical Electronic Information Technologies Jump to: navigation, search Name: VDE (Association for Electrical, Electronic & Information Technologies) Place:...

  9. Environmental Management Associates | Open Energy Information

    Open Energy Info (EERE)

    Environmental Management Associates Jump to: navigation, search Name: Environmental Management Associates Abbreviation: EMA Website: www.emacorp.com Environmental Management...

  10. Conventional Energy Forum & Associated Vertical Business Development...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Conventional Energy Forum & Associated Vertical Business Development: Best Practices in Indian Country Conventional Energy Forum & Associated Vertical Business Development: Best ...

  11. Voluntary Protection programs Participants' Association - Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Protection Programs Participants' Association (VPPPA) Presentations: Star Track Voluntary Protection Programs Participants' Association (VPPPA) Presentation: Wellness Programs

  12. Postdoctoral Associates | Photosynthetic Antenna Research Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Postdoctoral Associates Postdoctoral Associates Meghan Barnhart-Dailey Postdoctoral Research Associate Read more about Meghan Barnhart-Dailey Reddy Muthyala Nagarjuna Postdoctoral Research Associate Read more about Reddy Muthyala Nagarjuna David Swainsbury Postdoctoral Research Associate Read more about David Swainsbury Amit Mandal Postdoctoral Research Associate Read more about Amit Mandal Xun Lu Postdoctoral Research Associate Read more about Xun Lu Durgesh Rai Postdoctoral Research Associate

  13. NNSA Procurement Projects Perspective - Bob Raines, Associate...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Projects Perspective - Bob Raines, Associate Administrator for Acquisition and Project Management, NNSA NNSA Procurement Projects Perspective - Bob Raines, Associate Administrator ...

  14. Ethanol Steam Reforming on Co/CeO2: The Effect of ZnO Promoter...

    Office of Scientific and Technical Information (OSTI)

    Ethanol Steam Reforming on CoCeO2: The Effect of ZnO Promoter Citation Details In-Document Search Title: Ethanol Steam Reforming on CoCeO2: The Effect of ZnO Promoter A series of ...

  15. Orbital fluid shear stress promotes osteoblast metabolism, proliferation and alkaline phosphates activity in vitro

    SciTech Connect (OSTI)

    Aisha, M.D.; Nor-Ashikin, M.N.K.; Sharaniza, A.B.R.; Nawawi, H.; Froemming, G.R.A.

    2015-09-10

    Prolonged disuse of the musculoskeletal system is associated with reduced mechanical loading and lack of anabolic stimulus. As a form of mechanical signal, the multidirectional orbital fluid shear stress transmits anabolic signal to bone forming cells in promoting cell differentiation, metabolism and proliferation. Signals are channeled through the cytoskeleton framework, directly modifying gene and protein expression. For that reason, we aimed to study the organization of Normal Human Osteoblast (NHOst) cytoskeleton with regards to orbital fluid shear (OFS) stress. Of special interest were the consequences of cytoskeletal reorganization on NHOst metabolism, proliferation, and osteogenic functional markers. Cells stimulated at 250 RPM in a shaking incubator resulted in the rearrangement of actin and tubulin fibers after 72 h. Orbital shear stress increased NHOst mitochondrial metabolism and proliferation, simultaneously preventing apoptosis. The ratio of RANKL/OPG was reduced, suggesting that orbital shear stress has the potential to inhibit osteoclastogenesis and osteoclast activity. Increase in ALP activity and OCN protein production suggests that stimulation retained osteoblast function. Shear stress possibly generated through actin seemed to hold an anabolic response as osteoblast metabolism and functional markers were enhanced. We hypothesize that by applying orbital shear stress with suitable magnitude and duration as a non-drug anabolic treatment can help improve bone regeneration in prolonged disuse cases. - Highlights: • OFS stress transmits anabolic signals to osteoblasts. • Actin and tubulin fibers are rearranged under OFS stress. • OFS stress increases mitochondrial metabolism and proliferation. • Reduced RANKL/OPG ratio in response to OFS inhibits osteoclastogenesis. • OFS stress prevents apoptosis and stimulates ALP and OCN.

  16. Washington State Energy-Efficiency Appliance Rebate Program (SEEARP) Spurs Local Economy, Promotes State-Wide Partnership

    Broader source: Energy.gov [DOE]

    State of Washington works to save money, spur economic activity, and promote energy-efficient appliance options.

  17. Hong Qin promoted to executive dean at the University of Science...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hong Qin promoted to executive dean at the University of Science and Technology of China ... University of Science and Technology of China (USTC), which named him executive dean of ...

  18. DOE Project Leads to New Alliance to Promote Low-Impact Drilling

    Broader source: Energy.gov [DOE]

    A project supported by the Office of Fossil Energy's National Energy Technology Laboratory (NETL) has given rise to a major new research consortium to promote advanced technology for low-impact oil and gas drilling.

  19. Methods for promoting wound healing and muscle regeneration with the cell signaling protein Nell1

    SciTech Connect (OSTI)

    Culiat, Cymbeline T

    2014-11-04

    The present invention provides methods for promoting wound healing and treating muscle atrophy in a mammal in need. The method comprises administering to the mammal a Nell1 protein or a Nell1 nucleic acid molecule.

  20. Methods for promoting wound healing and muscle regeneration with the cell signaling protein Nell1

    DOE Patents [OSTI]

    Culiat, Cymbeline T.

    2011-03-22

    The present invention provides methods for promoting wound healing and treating muscle atrophy in a mammal in need. The method comprises administering to the mammal a Nell1 protein or a Nell1 nucleic acid molecule.

  1. Role of copper promotion in precipitated iron Fischer-Tropsch catalysts

    SciTech Connect (OSTI)

    O`Brien, R.J.; Xu, L.; Davis, B.H.

    1996-10-01

    Slurry phase Fischer-Tropsch synthesis was conducted on precipitated iron-silicon catalysts. The affect of copper promotion on the activity and selectivity of carbon monoxide, hydrogen and syngas activated catalysts is presented. High activity and stability have been obtained for potassium promoted catalysts when operating at 270{degrees}C; however, it has been found that promotion with potassium and copper is essential to obtaining good activity in a wax producing mode at 230{degrees}C. Promotion with copper is critical to achieving good activity when pretreating catalysts with hydrogen or with syngas at high pressure. XRD and Mossbauer data indicate that copper facilitates the reduction of iron oxide to metallic iron and iron carbides during hydrogen and syngas pretreatments.

  2. Toward a comprehensive understanding of rideshare promotion: The use of evaluation research

    SciTech Connect (OSTI)

    Horan, T.A.

    1988-01-01

    With traffic congestion becoming a serious problem in many metropolitan areas, rideshare promotion is increasingly being viewed as an important congestion reduction strategy. Since the oil embargo of 1973-74, the primary governmental mechanism for promoting ridesharing has been through rideshare agencies (RSAs). These RSAs work mainly with local employers to promote ridesharing to employees. Recent evidence suggests that RSAs could be more effective if they worked to develop comprehensive employer rideshare programs, rather than focusing predominately on the provision of matchlists to employees. This study, drawing upon the theory-driven approach suggested by Chen and Rossi (1983), delineated a program model for a more comprehensive rideshare promotional effort, and then executed an evaluation based upon several hypothesized relationship. Results confirmed several of the hypothesized relationships. In particular, rideshare program implementation had a significant bivariate relationship with employee rideshare placement. Several expected (and unexpected) employer and employee variables also had significant relationships with rideshare placement.

  3. Pseudogene CYP4Z2P 3'UTR promotes angiogenesis in breast cancer...

    Office of Scientific and Technical Information (OSTI)

    Title: Pseudogene CYP4Z2P 3'UTR promotes angiogenesis in breast cancer Highlights: * A new ... were studied. * The mechanism provides new insights for the breast cancer progression. ...

  4. Department of Energy and Disney Join Forces to Promote Energy Savings

    Broader source: Energy.gov [DOE]

    WASHINGTON, DC - U.S. Department of Energy (DOE) Secretary Samuel W. Bodman today announced that DOE and The Walt Disney Company are cooperating in a nationwide campaign to promote energy...

  5. Will Dichtel > Associate Professor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Chemistry and Chemical Biology > Faculty Directory > The Energy Materials Center at Cornell Will Dichtel Associate Professor Chemistry and Chemical Biology Research Group Webpage wrd33@cornell.edu The Dichtel Group uses the tools of synthetic and supramolecular chemistry to develop structurally precise organic materials. Projects in the Dichtel Group involve organic and polymer chemistry, solution and solid-state characterization, nanofabrication, and electronic testing of materials.

  6. Associated Post Office Box 117

    Office of Legacy Management (LM)

    Associated Post Office Box 117 Oak Ridge Tennessee 37831 -01 17 Energy Enuronmerl! Systems DIWSIO~I September 7, 1990 ? -iAsWP / Fll ,- ,) 3 Mr. Alexander Williams Office of Environmental Restoration and Waste Management U.S. Department of Energy Washington, DC 20545 Subject: PRELIMINARY SITE VISIT - REVERE COPPER AND BRASS CORP. Dear Mr. Williams: This letter is in response to your request concerning the results of the preliminary site visit to the former Revere Copper and Brass Corporation

  7. Bradbury Science Museum Association Volunteer

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Volunteers Volunteers Want to get more involved with the museum? We have several opportunities to either become a volunteer or request one at your event. Contact Us thumbnail of Bradbury Science Museum (505) 667-4444 Email Bradbury Science Museum Association Volunteer This official non-profit partner of the Bradbury Science Museum provides many opportunities to be more involved with the Bradbury's programs through work such as researching collections items and giving docent tours of the Bradbury

  8. Secretary Chu Announces New Efforts to Promote Clean Energy in Tribal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Communities Secretary Chu Announces New Efforts to Promote Clean Energy in Tribal Communities Secretary Steven Chu WASHINGTON � U.S. Energy Secretary Steven Chu announced today two new initiatives to promote tribal energy development and continue strengthening the partnership between the Department of Energy and tribal nations. Up to $10 million will be available this year through DOE�s Tribal Energy Program to support the evaluation, development and deployment of energy efficiency and

  9. New DOE Reports on Smart Grid Technologies Seek to Promote Innovation,

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Privacy and Access | Department of Energy Reports on Smart Grid Technologies Seek to Promote Innovation, Privacy and Access New DOE Reports on Smart Grid Technologies Seek to Promote Innovation, Privacy and Access October 5, 2010 - 12:00am Addthis Washington, D.C. - In order to implement recommendations made in the Federal Communications Commission's National Broadband Plan, the Department of Energy released two reports today on important policy issues raised by Smart Grid technologies that

  10. Obama Administration Announces $12 Million i6 Green Investment to Promote

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Clean Energy Innovation and Job Creation | Department of Energy $12 Million i6 Green Investment to Promote Clean Energy Innovation and Job Creation Obama Administration Announces $12 Million i6 Green Investment to Promote Clean Energy Innovation and Job Creation September 29, 2011 - 2:22pm Addthis WASHINGTON, D.C. - The Obama Administration today announced the six winners of the i6 Green Challenge, an initiative to drive technology commercialization and entrepreneurship in support of a green

  11. Obama Adminstration Announces Launch of i6 Green Challenge to Promote Clean

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Innovation and Economic Growth | Department of Energy Adminstration Announces Launch of i6 Green Challenge to Promote Clean Energy Innovation and Economic Growth Obama Adminstration Announces Launch of i6 Green Challenge to Promote Clean Energy Innovation and Economic Growth March 10, 2011 - 12:00am Addthis WASHINGTON - The U.S. Department of Energy joined with the U.S. Commerce Department's Economic Development Administration (EDA) and its Office of Innovation and Entrepreneurship

  12. United States and Japan Sign Joint Nuclear Energy Action Plan to Promote

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Nuclear Energy Cooperation | Department of Energy Japan Sign Joint Nuclear Energy Action Plan to Promote Nuclear Energy Cooperation United States and Japan Sign Joint Nuclear Energy Action Plan to Promote Nuclear Energy Cooperation April 25, 2007 - 12:36pm Addthis WASHINGTON, DC - United States Department of Energy Secretary Samuel W. Bodman and Japan's Ministers Akira Amari, Bunmei Ibuki, and Taro Aso, this week presented to U.S. President George W. Bush and Japanese Prime Minister Shinzo

  13. Australia, Iceland and the U.S. Launch International Partnership to Promote

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Advanced Geothermal Technologies | Department of Energy Australia, Iceland and the U.S. Launch International Partnership to Promote Advanced Geothermal Technologies Australia, Iceland and the U.S. Launch International Partnership to Promote Advanced Geothermal Technologies August 28, 2008 - 3:20pm Addthis REYKJAVIK, ICELAND - The U.S. Department of Energy's (DOE) Acting Assistant Secretary for Policy and International Affairs Katharine Fredriksen, Australia's Ambassador to Iceland Sharyn

  14. Secretary Chu Announces Initiatives to Promote Clean Energy at First Clean

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Ministerial | Department of Energy Initiatives to Promote Clean Energy at First Clean Energy Ministerial Secretary Chu Announces Initiatives to Promote Clean Energy at First Clean Energy Ministerial July 20, 2010 - 12:00am Addthis Washington, D.C. - At the world's first Clean Energy Ministerial, U.S. Energy Secretary Steven Chu today announced that the United States is helping launch more than 10 international clean energy initiatives. These initiatives will cut energy waste; help

  15. Energy Department Awards Will Promote Electric Vehicles in 24 States and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Train a Workforce for Advanced Vehicle Development | Department of Energy Will Promote Electric Vehicles in 24 States and Train a Workforce for Advanced Vehicle Development Energy Department Awards Will Promote Electric Vehicles in 24 States and Train a Workforce for Advanced Vehicle Development September 8, 2011 - 3:17pm Addthis Washington, D.C. - U.S. Energy Secretary Steven Chu today announced 16 projects supporting activities in 24 states and the District of Columbia to accelerate the

  16. Obama Administration Announces Launch of i6 Green Challenge to Promote

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Clean Energy Innovation and Economic Growth | Department of Energy Obama Administration Announces Launch of i6 Green Challenge to Promote Clean Energy Innovation and Economic Growth Obama Administration Announces Launch of i6 Green Challenge to Promote Clean Energy Innovation and Economic Growth March 10, 2011 - 4:59pm Addthis The U.S. Department of Energy joined with the U.S. Commerce Department's Economic Development Administration (EDA) and its Office of Innovation and Entrepreneurship

  17. California Member Marches in July 4th Parade to Promote Home Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Upgrades | Department of Energy Marches in July 4th Parade to Promote Home Energy Upgrades California Member Marches in July 4th Parade to Promote Home Energy Upgrades Photo of people marching in a parade with signs, wearing matching shirts. Residential Network member Community Home Energy Retrofit Project (CHERP) marched in the Claremont, California, Independence Day parade on July 4, 2015, to raise community awareness of home energy upgrades. An email campaign sent prior to the parade

  18. Oak Rigde Associated Universities (ORAU) Radiation Emergency...

    Broader source: Energy.gov (indexed) [DOE]

    Rigde Associated Universities (ORAU) Radiation Emergency Assistance CenterTraining Site (REACTS), ORAU Director Oak Rigde Associated Universities (ORAU) Radiation Emergency ...

  19. National Association of Tribal Historic Preservation Officers...

    Open Energy Info (EERE)

    National Association of Tribal Historic Preservation Officers website Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: National Association of Tribal...

  20. Global Change Associates | Open Energy Information

    Open Energy Info (EERE)

    Change Associates Jump to: navigation, search Name: Global Change Associates Place: New York City, New York Zip: 10025-5657 Product: Consulting firm focusing on the convergence...

  1. BCL Associates Inc | Open Energy Information

    Open Energy Info (EERE)

    BCL Associates Inc Jump to: navigation, search Name: BCL & Associates Inc Place: Desert Hot Springs, California Zip: 92240 Product: Private company whic has filed to develop PV...

  2. California Solar Energy Industries Association | Open Energy...

    Open Energy Info (EERE)

    Solar Energy Industries Association Jump to: navigation, search Name: California Solar Energy Industries Association Place: Rio Vista, California Zip: 94571 Sector: Solar Product:...

  3. Wind Power Associates LLC | Open Energy Information

    Open Energy Info (EERE)

    Power Associates LLC Jump to: navigation, search Name: Wind Power Associates LLC Place: Goldendale, Washington State Sector: Wind energy Product: Wind farm developer and operater....

  4. Electrochemical Design Associates EDA | Open Energy Information

    Open Energy Info (EERE)

    Design Associates EDA Jump to: navigation, search Name: Electrochemical Design Associates (EDA) Place: California Zip: 94608-2513 Product: EDA is a technology development company...

  5. Preliminary Notice of Violation, Brookhaven Science Associates...

    Office of Environmental Management (EM)

    Brookhaven Science Associates, LLC - WEA-2013-01 Preliminary Notice of Violation, Brookhaven Science Associates, LLC - WEA-2013-01 August 29, 2013 Issued to Brookhaven Science ...

  6. Nationwide: National Fire Protection Association Provides Training...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    National Fire Protection Association Provides Training to First Responders on EVsPEVs Nationwide: National Fire Protection Association Provides Training to First Responders on...

  7. Nationwide: National Fire Protection Association Provides Training...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Nationwide: National Fire Protection Association Provides Training to First Responders on EVsPEVs Nationwide: National Fire Protection Association Provides Training to First...

  8. Biomass Power Association (BPA) | Open Energy Information

    Open Energy Info (EERE)

    Summary LAUNCH TOOL Name: Biomass Power Association (BPA) AgencyCompany Organization: Biomass Power Association Sector: Energy Focus Area: Biomass, - Biomass Combustion, -...

  9. Solar Energy Industries Association | Open Energy Information

    Open Energy Info (EERE)

    Solar Energy Industries Association Name: Solar Energy Industries Association Address: 575 7th Street NW 400 Place: Washington, DC Zip: 20004 Number of Employees: 11-50 Year...

  10. Solar Design Associates Inc | Open Energy Information

    Open Energy Info (EERE)

    Associates Inc Jump to: navigation, search Name: Solar Design Associates Inc Address: P.O. Box 242 Place: Harvard, Massachusetts Zip: 01451 Region: Greater Boston Area Sector:...

  11. National Hydropower Association | Open Energy Information

    Open Energy Info (EERE)

    Hydropower Association Jump to: navigation, search Name: National Hydropower Association Place: Washington, DC Zip: 20001 Sector: Hydro Product: NHA is a non-profit national...

  12. China Wind Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Wind Energy Association Jump to: navigation, search Name: China Wind Energy Association Place: Beijing, Beijing Municipality, China Zip: 100013 Sector: Wind energy Product: A...

  13. Nevada Archaeological Association Website | Open Energy Information

    Open Energy Info (EERE)

    Association Website Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: Nevada Archaeological Association Website Abstract Provides information about...

  14. Potential of Diazorphic, Endophytic Bacteria Associated with...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Potential of Diazorphic, Endophytic Bacteria Associated with Sugarcane for Energycane Production Potential of Diazorphic, Endophytic Bacteria Associated with Sugarcane for Energycane ...

  15. Steven Winter Associates (Consortium for Advanced Residential...

    Open Energy Info (EERE)

    Steven Winter Associates (Consortium for Advanced Residential Buildings) Jump to: navigation, search Name: Steven Winter Associates (Consortium for Advanced Residential Buildings)...

  16. Renewable Energy Developers Association of Maharashtra | Open...

    Open Energy Info (EERE)

    Association of Maharashtra Jump to: navigation, search Name: Renewable Energy Developers Association of Maharashtra Place: Mumbai, Maharashtra, India Sector: Renewable Energy...

  17. Danish Wind Industry Association | Open Energy Information

    Open Energy Info (EERE)

    Jump to: navigation, search Name: Danish Wind Industry Association Place: Copenhagen V, Denmark Zip: DK-1552 Sector: Wind energy Product: The Danish Wind Industry Association...

  18. Spanish Association for the Internationalisation and Innovation...

    Open Energy Info (EERE)

    Association for the Internationalisation and Innovation of Solar Companies Jump to: navigation, search Name: Spanish Association for the Internationalisation and Innovation of...

  19. National Hydrogen Association | Open Energy Information

    Open Energy Info (EERE)

    Hydrogen Association Jump to: navigation, search Name: National Hydrogen Association Place: Washington, Washington, DC Zip: 20036 Sector: Hydro, Hydrogen Product: The source for...

  20. German Hydrogen Association DWV | Open Energy Information

    Open Energy Info (EERE)

    Hydrogen Association DWV Jump to: navigation, search Name: German Hydrogen Association (DWV) Place: Berlin, Germany Zip: 12205 Sector: Hydro, Hydrogen Product: String...

  1. Geothermal Energy Association Recognizes the National Geothermal...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Geothermal Energy Association Recognizes the National Geothermal Data System Geothermal Energy Association Recognizes the National Geothermal Data System July 29, 2014 - 8:20am...

  2. Secure Smart Grid Association | Open Energy Information

    Open Energy Info (EERE)

    Smart Grid Association Jump to: navigation, search Name: Secure Smart Grid Association Address: 2374 S Josephine St Place: Denver, Colorado Zip: 80210 Region: Rockies Area Number...

  3. British Photovoltaic Association | Open Energy Information

    Open Energy Info (EERE)

    Photovoltaic Association Jump to: navigation, search Name: British Photovoltaic Association Place: Milton Keynes, United Kingdom Zip: MK5 8NG Product: Trade body for the PV...

  4. Geysers Geothermal Association GGA | Open Energy Information

    Open Energy Info (EERE)

    GGA Jump to: navigation, search Name: Geysers Geothermal Association (GGA) Place: Santa Rosa, California Zip: 95404 Sector: Geothermal energy Product: Trade association...

  5. Donald Aitken Associates | Open Energy Information

    Open Energy Info (EERE)

    Aitken Associates Jump to: navigation, search Name: Donald Aitken Associates Place: Berkeley, California Zip: 94507 Product: Environmental consultants and sustainable development...

  6. Layman Energy Associates | Open Energy Information

    Open Energy Info (EERE)

    Energy Associates Jump to: navigation, search Name: Layman Energy Associates Place: San Luis Obispo, California Zip: 93405 Sector: Geothermal energy Product: A...

  7. Distributed Wind Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Energy Association Jump to: navigation, search Name: Distributed Wind Energy Association Address: PO Box 1861 Place: Flagstaff, AZ Zip: 86002 Phone Number: 928-255-0214 Website:...

  8. Apolipoprotein E promotes lipid accumulation and differentiation in human adipocytes

    SciTech Connect (OSTI)

    Lasrich, Dorothee; Bartelt, Alexander; Grewal, Thomas; Heeren, Joerg

    2015-09-10

    Several studies in mice indicate a role for apolipoprotein E (APOE) in lipid accumulation and adipogenic differentiation in adipose tissue. However, little is yet known if APOE functions in a similar manner in human adipocytes. This prompted us to compare lipid loading and expression of adipocyte differentiation markers in APOE-deficient and control adipocytes using the differentiated human mesenchymal stem cell line hMSC-Tert as well as primary human and mouse adipocytes as model systems. Differentiated hMSC-Tert were stably transduced with or without siRNA targeting APOE while murine adipocytes were isolated from wild type and Apoe knockout mice. Human APOE knockdown hMSC-Tert adipocytes accumulated markedly less triglycerides compared to control cells. This correlated with strongly decreased gene expression levels of adipocyte markers such as adiponectin (ADIPOQ) and fatty acid binding protein 4 (FABP4) as well as the key transcription factor driving adipocyte differentiation, peroxisome proliferator activator receptor gamma (PPARG), in particular the PPARG2 isoform. Similarly, differentiation of murine Apoe-deficient adipocytes was characterized by reduced gene expression of Adipoq, Fabp4 and Pparg. Interestingly, incubation of APOE-deficient hMSC-Tert adipocytes with conditioned media from APOE3-overexpressing adipocytes or APOE-containing Very Low Density Lipoprotein (VLDL) partially restored triglyceride accumulation, but were unable to induce adipocyte differentiation, as judged by expression of adipocyte markers. Taken together, depletion of endogenous APOE in human adipocytes severely impairs lipid accumulation, which is associated with an inability to initiate differentiation. - Highlights: • Immortalized human mesenchymal stem cells were used to study adipocyte development. • Knockdown of endogenous APOE lead to impaired lipid accumulation and adipogenesis. • APOE supplementation partially restored lipid accumulation but not differentiation.

  9. Designing PV Incentive Programs to Promote System Performance: AReview of Current Practice

    SciTech Connect (OSTI)

    Barbose, Galen; Wiser, Ryan; Bolinger, Mark

    2006-11-12

    Some stakeholders continue to voice concerns about the performance of customer-sited photovoltaic (PV) systems, particularly because these systems typically receive financial support through ratepayer- or publicly-funded programs. Although much remains to be understood about the extent and specific causes of poor PV system performance, several studies of the larger programs and markets have shed some light on the issue. An evaluation of the California Energy Commission (CEC)'s Emerging Renewables Program, for example, found that 7% of systems, in a sample of 95, had lower-than-expected power output due to shading or soiling (KEMA 2005). About 3% of a larger sample of 140 systems were not operating at all or were operating well below expected output, due to failed equipment, faulty installation workmanship, and/or a lack of basic maintenance. In a recent evaluation of the other statewide PV incentive program in California, the Self-Generation Incentive Program, 9 of 52 projects sampled were found to have annual capacity factors less than 14.5%, although reasons for these low capacity factors generally were not identified (Itron 2005). Studies of PV systems in Germany and Japan, the two largest PV markets worldwide, have also revealed some performance problems associated with issues such as shading, equipment and installation defects, inverter failure, and deviations from module manufacturers' specifications (Otani et al. 2004, Jahn & Nasse 2004). Although owners of PV systems have an inherent incentive to ensure that their systems perform well, many homeowners and building operators may lack the necessary information and expertise to carry out this task effectively. Given this barrier, and the responsibility of PV incentive programs to ensure that public funds are prudently spent, these programs should (and often do) play a critical role in promoting PV system performance. Performance-based incentives (PBIs), which are based on actual energy production rather than

  10. Using the apparent diffusion coefficient to identifying MGMT promoter methylation status early in glioblastoma: importance of analytical method

    SciTech Connect (OSTI)

    Rundle-Thiele, Dayle; Day, Bryan; Stringer, Brett; Fay, Michael; Martin, Jennifer; Jeffree, Rosalind L; Thomas, Paul; Bell, Christopher; Salvado, Olivier; Gal, Yaniv; Coulthard, Alan; Crozier, Stuart; Rose, Stephen

    2015-06-15

    Accurate knowledge of O{sup 6}-methylguanine methyltransferase (MGMT) gene promoter subtype in patients with glioblastoma (GBM) is important for treatment. However, this test is not always available. Pre-operative diffusion MRI (dMRI) can be used to probe tumour biology using the apparent diffusion coefficient (ADC); however, its ability to act as a surrogate to predict MGMT status has shown mixed results. We investigated whether this was due to variations in the method used to analyse ADC. We undertook a retrospective study of 32 patients with GBM who had MGMT status measured. Matching pre-operative MRI data were used to calculate the ADC within contrast enhancing regions of tumour. The relationship between ADC and MGMT was examined using two published ADC methods. A strong trend between a measure of ‘minimum ADC’ and methylation status was seen. An elevated minimum ADC was more likely in the methylated compared to the unmethylated MGMT group (U = 56, P = 0.0561). In contrast, utilising a two-mixture model histogram approach, a significant reduction in mean measure of the ‘low ADC’ component within the histogram was associated with an MGMT promoter methylation subtype (P < 0.0246). This study shows that within the same patient cohort, the method selected to analyse ADC measures has a significant bearing on the use of that metric as a surrogate marker of MGMT status. Thus for dMRI data to be clinically useful, consistent methods of data analysis need to be established prior to establishing any relationship with genetic or epigenetic profiling.

  11. ENO1 promotes tumor proliferation and cell adhesion mediated drug resistance (CAM-DR) in Non-Hodgkin's Lymphomas

    SciTech Connect (OSTI)

    Zhu, Xinghua; Miao, Xiaobing; Wu, Yaxun; Li, Chunsun; Guo, Yan; Liu, Yushan; Chen, Yali; Lu, Xiaoyun; Wang, Yuchan; He, Song

    2015-07-15

    Enolases are glycolytic enzymes responsible for the ATP-generated conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to the glycolytic function, Enolase 1 (ENO1) has been reported up-regulation in several tumor tissues. In this study, we investigated the expression and biologic function of ENO1 in Non-Hodgkin's Lymphomas (NHLs). Clinically, by western blot analysis we observed that ENO1 expression was apparently higher in diffuse large B-cell lymphoma than in the reactive lymphoid tissues. Subsequently, immunohistochemical staining of 144 NHLs suggested that the expression of ENO1 was significantly lower in the indolent lymphomas compared with the progressive lymphomas. Further, we identified ENO1 as an independent prognostic factor, and it was significantly correlated with overall survival of NHL patients. In addition, we found that ENO1 could promote cell proliferation, regulate cell cycle associated gene and PI3K/AKT signaling pathway in NHLs. Finally, we verified that ENO1 participated in the process of lymphoma cell adhesion mediated drug resistance (CAM-DR). Adhesion to FN or HS5 cells significantly protected OCI-Ly8 and Daudi cells from cytotoxicity compared with those cultured in suspension, and these effects were attenuated when transfected with ENO1-siRNA. Based on the study, we propose that inhibition of ENO1 expression may be a novel strategy for therapy for NHLs patients, and it may be a target for drug resistance. - Highlights: • ENO1 expression is reversely correlated with clinical outcomes of patients with NHLs. • ENO1 promotes the proliferation of NHL cells. • ENO1 regulates cell adhesion mediated drug resistance.

  12. NA 80 - Associate Administrator for Counterterrorism and

    National Nuclear Security Administration (NNSA)

    Counterproliferation | National Nuclear Security Administration | (NNSA) 80 - Associate Administrator for Counterterrorism and Counterproliferation

  13. QER- Comment of Northeast Gas Association

    Broader source: Energy.gov [DOE]

    Please find enclosed comments of the Northeast Gas Association regarding the Quadrennial Energy Review. Thank you.

  14. 2009 Voluntary Protection Programs Participants' Association (VPPPA)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Presentations: Star Track | Department of Energy Presentations: Star Track 2009 Voluntary Protection Programs Participants' Association (VPPPA) Presentations: Star Track 2009 Voluntary Protection Programs Participants' Association (VPPPA) Presentations: Star Track 2009 Voluntary Protection Programs Participants' Association (VPPPA) Presentations: Star Track (5.63 MB) More Documents & Publications 2009 Voluntary Protection Programs Participants' Association (VPPPA) Presentation: How to

  15. Voluntary Protection Programs Participants' Association (VPPPA)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Presentation: KCSO VPP Process | Department of Energy Programs Participants' Association (VPPPA) Presentation: KCSO VPP Process Voluntary Protection Programs Participants' Association (VPPPA) Presentation: KCSO VPP Process Voluntary Protection Programs Participants' Association (VPPPA) Presentation: KCSO VPP Process Voluntary Protection Programs Participants' Association (VPPPA) Presentation: KCSO VPP Process (981.65 KB) More Documents & Publications 2009 Voluntary Protection Programs

  16. Oak Ridge Associated Universities Procurement Questionnaire Application

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    System Supplier Profile PIA, Oak ridge Operations Office | Department of Energy Associated Universities Procurement Questionnaire Application System Supplier Profile PIA, Oak ridge Operations Office Oak Ridge Associated Universities Procurement Questionnaire Application System Supplier Profile PIA, Oak ridge Operations Office Oak Ridge Associated Universities Procurement Questionnaire Application System Supplier Profile PIA, Oak ridge Operations Office Oak Ridge Associated Universities

  17. Associations between cadmium exposure and circulating levels of sex hormones in postmenopausal women

    SciTech Connect (OSTI)

    Ali, Imran; Engström, Annette; Vahter, Marie; Skerfving, Staffan; Lundh, Thomas; Lidfeldt, Jonas; Samsioe, Göran; Halldin, Krister; Åkesson, Agneta

    2014-10-15

    Recent epidemiological as well as in vivo and in vitro studies collectively suggest that the metalloestrogen cadmium (Cd) could be a potential risk factor for hormone-related cancers in particularly breast cancer. Assessment of the association between Cd exposure and levels of endogenous sex hormones is of pivotal importance, as increased levels of such have been associated with a higher risk of breast cancer in postmenopausal women. The present study investigated the perceived relationship (multivariable-adjusted linear regression analyses) between Cd exposure [blood Cd (B-Cd) and urinary Cd (U-Cd)], and serum levels of androstenedione, testosterone, estradiol, and sex-hormone binding globulin (SHBG), in 438 postmenopausal Swedish women without hormone replacement therapy (HRT). A significant positive association between B-Cd (median 3.4 nmol/L) and serum testosterone levels, as well as a significant inverse association between B-Cd and serum estradiol levels and with the estradiol/testosterone ratio were encountered. However, U-Cd (median 0.69 nmol/mmol creatinine) was inversely associated with serum estradiol levels only. Our data may suggest that Cd interferes with the levels of testosterone and estradiol in postmenopausal women, which might have implications for breast cancer risk. - Highlights: • Low level cadmium exposure may interfere with the levels of steroid hormones. • Cadmium exposure was associated with increased serum testosterone concentrations. • Cadmium exposure was associated with decreased estradiol/testosterone ratio. • Cadmium exposure may have implications for breast-cancer promotion.

  18. Regenerable MgO promoted metal oxide oxygen carriers for chemical looping combustion

    DOE Patents [OSTI]

    Siriwardane, Ranjani V.; Miller, Duane D.

    2014-08-19

    The disclosure provides an oxygen carrier comprised of a plurality of metal oxide particles in contact with a plurality of MgO promoter particles. The MgO promoter particles increase the reaction rate and oxygen utilization of the metal oxide when contacting with a gaseous hydrocarbon at a temperature greater than about 725.degree. C. The promoted oxide solid is generally comprised of less than about 25 wt. % MgO, and may be prepared by physical mixing, incipient wetness impregnation, or other methods known in the art. The oxygen carrier exhibits a crystalline structure of the metal oxide and a crystalline structure of MgO under XRD crystallography, and retains these crystalline structures over subsequent redox cycles. In an embodiment, the metal oxide is Fe.sub.2O.sub.3, and the gaseous hydrocarbon is comprised of methane.

  19. Protein kinase C and its substrates in tumor promoter-sensitive and -resistant cells

    SciTech Connect (OSTI)

    Smith, B.M.; Colburn, N.H.

    1988-05-05

    Calcium- and phospholipid-dependent protein kinase C activity and substrates were characterized in cell lysates of preneoplastic JB6 cells, a model system of genetic variants for sensitivity to tumor promoter-induced neoplastic transformation. Protein kinase C activity was similar for sensitive and resistant variants, as measured by calcium- and phospholipid-dependent phosphorylation of an exogenous substrate (histone HIII). Of 13 endogenous protein kinase C substrates, identified by labeling proteins with (..gamma..-/sup 32/P) ATP, at least two (80 and 23 kDa) are potential candidates for mediating events on the pathway for promotion of transformation. /sup 32/P incorporation into the 80-kDa protein kinase C substrate was stimulated by tetradecanoylphorbol acetate and correlated with phenotype: the highest incorporation was found in promotion-insensitive cells, an intermediate level in promotion-sensitive cells and the lowest in the transformed cells. The phosphorylation of an 80-kDa protein, found by labeling intact cells in monolayer growth with (/sup 32/P)orthophosphate, was also stimulated by tetradecanoylphorbol acetate and correlated inversely with phenotype. The 80 kDa protein kinase C substrate from cells lysates and the 80-kDa phosphoprotein from intact cells appear to be identical, as indicated by peptide mapping with protease V8 from Staphylococcus aureus. This finding suggests that the 80-kDa substrate is relevant to promoter-induced signal transduction in the intact cell. In summary, there are no unique substrates that distinguish the variants. Quantitative differences in certain substrates or their phosphorylation may, however, account for the difference in promotion sensitivity among the variants.

  20. U.S. Department of Energy and the Automotive X PRIZE Foundation to Promote

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Clean, Energy-Efficient Vehicles | Department of Energy the Automotive X PRIZE Foundation to Promote Clean, Energy-Efficient Vehicles U.S. Department of Energy and the Automotive X PRIZE Foundation to Promote Clean, Energy-Efficient Vehicles March 20, 2008 - 10:52am Addthis DOE to invest $3.5 million in public outreach effort NEW YORK, NY - In an effort to engage students and the public on the significance of increasing the use of more clean, cutting-edge and energy-efficient vehicles to

  1. Isolated Polynucleotides and Methods of Promoting a Morphology in a Fungus

    SciTech Connect (OSTI)

    Lasure, Linda L; Dai, Ziyu

    2008-10-21

    The invention includes isolated polynucleotide molecules that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention includes a method of enhancing a bioprocess utilizing a fungus. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to a promoter. The polynucleotide sequence is expressed to promote a first morphology. The first morphology of the transformed fungus enhances a bioprocess relative to the bioprocess utilizing a second morphology.

  2. Promoting Offshore Wind Along the "Fresh Coast" | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Promoting Offshore Wind Along the "Fresh Coast" Promoting Offshore Wind Along the "Fresh Coast" October 12, 2010 - 12:18pm Addthis Chris Hart Offshore Wind Team Lead, Wind & Water Power Program When people think about offshore wind power, the first location that comes to mind probably isn't Cleveland, Ohio. Most of the offshore wind turbines installed around the world are operating in salt water, like Europe's North Sea and Baltic Sea, and most of the offshore wind

  3. DOE Awards up to $5.5 Million for X PRIZE to Promote Clean, Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Efficient Vehicles | Department of Energy up to $5.5 Million for X PRIZE to Promote Clean, Energy Efficient Vehicles DOE Awards up to $5.5 Million for X PRIZE to Promote Clean, Energy Efficient Vehicles November 2, 2009 - 12:00am Addthis WASHINGTON, DC - Energy Secretary Steven Chu today announced that the Department of Energy is providing up to $5.5 million in funding from the American Recovery and Reinvestment Act to support the X PRIZE Foundation's work to inspire a new generation of

  4. Good practices to promote gender equality in science and physics in Tunisia

    SciTech Connect (OSTI)

    Jaziri, Sihem

    2015-12-31

    Women represent 47% of researchers in Tunisia but only 12% of senior faculty in scientific fields. This paper describes three areas of activity to promote greater participation of women. The Women in Science group at the University of Tunis has, among other activities, organized an annual conference on women in science since 2005. The COACh project is a multinational effort across northern Africa to promote professional networking and mentoring through partnerships with women in the United States. Finally, an immersive summer school was organized to introduce master’s level women to projects and careers in optics and photonics.

  5. ECRbase: Database of Evolutionary Conserved Regions, Promoters, and Transcription Factor Binding Sites in Vertebrate Genomes

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Loots, Gabriela G. [LLNL; Ovcharenko, I. [LLNL

    Evolutionary conservation of DNA sequences provides a tool for the identification of functional elements in genomes. This database of evolutionary conserved regions (ECRs) in vertebrate genomes features a database of syntenic blocks that recapitulate the evolution of rearrangements in vertebrates and a comprehensive collection of promoters in all vertebrate genomes generated using multiple sources of gene annotation. The database also contains a collection of annotated transcription factor binding sites (TFBSs) in evolutionary conserved and promoter elements. ECRbase currently includes human, rhesus macaque, dog, opossum, rat, mouse, chicken, frog, zebrafish, and fugu genomes. (taken from paper in Journal: Bioinformatics, November 7, 2006, pp. 122-124

  6. Benton Falls Associates | Open Energy Information

    Open Energy Info (EERE)

    Falls Associates Place: Maine Sector: Hydro Product: Owns and operates a 4.3MW hydroelectric plant in Benton. References: Benton Falls Associates1 This article is a stub....

  7. Postdoctoral Associates | Photosynthetic Antenna Research Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Alastair Gardiner Research Associate E-mail: alastair.gardiner@glasgow.ac.uk Phil Jackson Phil Jackson Postdoctoral Research Associate E-mail: p.j.jackson@sheffield.ac.uk...

  8. QER- Comment of Canadian Gas Association

    Broader source: Energy.gov [DOE]

    SENT ON BEHALF OF TIMOTHY M. EGAN PRESIDENT AND CEO CANADIAN GAS ASSOCIATION Dear Quadrennial Energy Review Task Force, Please find attached the submission from the Canadian Gas Association to the Quadrennial Energy Review (QER) process.

  9. K C Electric Association | Open Energy Information

    Open Energy Info (EERE)

    K C Electric Association Jump to: navigation, search Name: K C Electric Association Place: Colorado Website: www.kcelectric.coop Outage Hotline: 1-800-700-3123 Outage Map:...

  10. Mora Associates Ltd | Open Energy Information

    Open Energy Info (EERE)

    Mora Associates Ltd Jump to: navigation, search Name: Mora Associates Ltd Place: London, Greater London, United Kingdom Zip: WC2H 9UY Product: London-based advisory firm helping...

  11. 2009 Voluntary Protection Programs Participants' Association...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Presentation: Conducting your Annual VPP Self Assessment 2009 Voluntary Protection Programs Participants' Association (VPPPA) Presentation: Conducting your Annual VPP Self ...

  12. Stearns Electric Association - Commercial Energy Efficiency Rebate...

    Broader source: Energy.gov (indexed) [DOE]

    Electric Association Website https:www.stearnselectric.orgconservation-incentivesenergy-efficienc... State Minnesota Program Type Rebate Program Rebate Amount Variable...

  13. QER- Comment of Geotherman Energy Association (GEA)

    Broader source: Energy.gov [DOE]

    Hi the Geothermal Energy Association would like to submit the attached comments for the 2014 QER process. Thanks,

  14. Moore honored with American Statistical Association award

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    American Statistical Association Award Moore honored with American Statistical Association award Lisa Moore is the recipient of the 2013 Don Owen Award presented by the American Statistical Association, San Antonio Chapter. May 24, 2013 Leslie "Lisa" Moore Leslie "Lisa" Moore The American Statistical Association (ASA) is the world's largest community of statisticians. It was founded in Massachusetts in 1839. Leslie "Lisa" Moore of the Laboratory's Statistical

  15. China Association of Resource Comprehensive Utilisation CARCU...

    Open Energy Info (EERE)

    of Resource Comprehensive Utilisation CARCU Jump to: navigation, search Name: China Association of Resource Comprehensive Utilisation (CARCU) Place: Beijing Municipality,...

  16. National Science Teachers Association Annual Conference

    Broader source: Energy.gov [DOE]

    The National Science Teachers Association annual conference was held in Chicago, Illinois, from March 12–15, 2015.

  17. American Gas Association | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Association American Gas Association Memorandum Summarizing Ex Parte Communication 111011_Ex_Parte.pdf (691.79 KB) More Documents & Publications Ex Parte Memorandum - Natural Resources Defense Council American Gas Association Ex Parte Communication Microsoft Word - AGA Comments on 2011 Regulatory Burden RFI

  18. 2009 Voluntary Protection Programs Participants' Association (VPPPA)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Presentation: Conducting your Annual VPP Self Assessment | Department of Energy Presentation: Conducting your Annual VPP Self Assessment 2009 Voluntary Protection Programs Participants' Association (VPPPA) Presentation: Conducting your Annual VPP Self Assessment 2009 Voluntary Protection Programs Participants' Association (VPPPA) Presentation: Conducting your Annual VPP Self Assessment 2009 Voluntary Protection Programs Participants' Association (VPPPA) Presentation: Conducting your Annual

  19. 2009 Voluntary Protection Programs Participants' Association (VPPPA)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Presentation: How to communicate | Department of Energy Presentation: How to communicate 2009 Voluntary Protection Programs Participants' Association (VPPPA) Presentation: How to communicate 2009 Voluntary Protection Programs Participants' Association (VPPPA) Presentation: How to communicate 2009 Voluntary Protection Programs Participants' Association (VPPPA) Presentation: How to communicate (7.97 MB) More Documents & Publications 2009 Voluntary Protection Programs Participants'

  20. Voluntary Protection programs Participants' Association - Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Memorandum of Understanding | Department of Energy programs Participants' Association - Department of Energy Memorandum of Understanding Voluntary Protection programs Participants' Association - Department of Energy Memorandum of Understanding This Memorandum of Understanding (MOU) states the understandings between the Voluntary Protection programs Participants' Association (VPPPA) and U.S. Department of Energy (DOE) under which the parties agree to work cooperatively to address

  1. Use of CYP52A2A promoter to increase gene expression in yeast

    DOE Patents [OSTI]

    Craft, David L.; Wilson, C. Ron; Eirich, Dudley; Zhang, Yeyan

    2004-01-06

    A nucleic acid sequence including a CYP promoter operably linked to nucleic acid encoding a heterologous protein is provided to increase transcription of the nucleic acid. Expression vectors and host cells containing the nucleic acid sequence are also provided. The methods and compositions described herein are especially useful in the production of polycarboxylic acids by yeast cells.

  2. Acid Strength and Bifunctional Catalytic Behavior of Alloys Comprised of Noble Metals and Oxophilic Metal Promoters

    SciTech Connect (OSTI)

    Hibbitts, David D.; Tan, Qiaohua; Neurock, Matthew

    2014-06-01

    The promotion of metal catalysts with partially oxidized oxophilic MOx species, such as ReOx-promoted Rh, has been demonstrated to produce Brnsted acid sites that can promote hydrogenolysis of oxygenate intermediates such as those found in biomass-derived species. A wide variety of alloy compositions and structures are examined in this work to investigate strongly acidic promoters by using DFT-calculated deprotonation energies (DPE) as a measure of acid strength. Sites with the highest acid strength had DPE less than 1100 kJ mol-1, similar to DPE values of heteropolyacids or acid-containing zeolites, and were found on alloys composed of an oxophilic metal (such as Re or W) with a noble metal (such as Rh or Pt). NH3 adsorbs more strongly to sites with increasing acid strength and the activation barriers for acid-catalyzed ring opening of a furan ring decrease with increasing acid strength, which was also shown to be stronger for OH acid sites bound to multiple oxophilic metal atoms in a three-fold configuration rather than OH sites adsorbed in an atop configuration on one oxophilic metal, indicating that small MOx clusters may yield sites with the highest acid strength.

  3. Promoting electricity from renewable energy sources -- lessons learned from the EU, U.S. and Japan

    SciTech Connect (OSTI)

    Haas, Reinhard; Meyer, Niels I.; Held, Anne; Finon, Dominique; Lorenzoni, Arturo; Wiser, Ryan; Nishio, Ken-ichiro

    2007-06-01

    The promotion of electricity generated from Renewable Energy Sources (RES) has recently gained high priority in the energy policy strategies of many countries in response to concerns about global climate change, energy security and other reasons. This chapter compares and contrasts the experience of a number of countries in Europe, states in the US as well as Japan in promoting RES, identifying what appear to be the most successful policy measures. Clearly, a wide range of policy instruments have been tried and are in place in different parts of the world to promote renewable energy technologies. The design and performance of these schemes varies from place to place, requiring further research to determine their effectiveness in delivering the desired results. The main conclusions that can be drawn from the present analysis are: (1) Generally speaking, promotional schemes that are properly designed within a stable framework and offer long-term investment continuity produce better results. Credibility and continuity reduce risks thus leading to lower profit requirements by investors. (2) Despite their significant growth in absolute terms in a number of key markets, the near-term prognosis for renewables is one of modest success if measured in terms of the percentage of the total energy provided by renewables on a world-wide basis. This is a significant challenge, suggesting that renewables have to grow at an even faster pace if we expect them to contribute on a significant scale to the world's energy mix.

  4. Sirtuin 7 promotes cellular survival following genomic stress by attenuation of DNA damage, SAPK activation and p53 response

    SciTech Connect (OSTI)

    Kiran, Shashi; Oddi, Vineesha; Ramakrishna, Gayatri

    2015-02-01

    Maintaining the genomic integrity is a constant challenge in proliferating cells. Amongst various proteins involved in this process, Sirtuins play a key role in DNA damage repair mechanisms in yeast as well as mammals. In the present work we report the role of one of the least explored Sirtuin viz., SIRT7, under conditions of genomic stress when treated with doxorubicin. Knockdown of SIRT7 sensitized osteosarcoma (U2OS) cells to DNA damage induced cell death by doxorubicin. SIRT7 overexpression in NIH3T3 delayed cell cycle progression by causing delay in G1 to S transition. SIRT7 overexpressing cells when treated with low dose of doxorubicin (0.25 µM) showed delayed onset of senescence, lesser accumulation of DNA damage marker γH2AX and lowered levels of growth arrest markers viz., p53 and p21 when compared to doxorubicin treated control GFP expressing cells. Resistance to DNA damage following SIRT7 overexpression was also evident by EdU incorporation studies where cellular growth arrest was significantly delayed. When treated with higher dose of doxorubicin (>1 µM), SIRT7 conferred resistance to apoptosis by attenuating stress activated kinases (SAPK viz., p38 and JNK) and p53 response thereby shifting the cellular fate towards senescence. Interestingly, relocalization of SIRT7 from nucleolus to nucleoplasm together with its co-localization with SAPK was an important feature associated with DNA damage. SIRT7 mediated resistance to doxorubicin induced apoptosis and senescence was lost when p53 level was restored by nutlin treatment. Overall, we propose SIRT7 attenuates DNA damage, SAPK activation and p53 response thereby promoting cellular survival under conditions of genomic stress. - Highlights: • Knockdown of SIRT7 sensitized cells to DNA damage induced apoptosis. • SIRT7 delayed onset of premature senescence by attenuating DNA damage response. • Overexpression of SIRT7 delayed cell cycle progression by delaying G1/S transition. • Upon DNA damage SIRT

  5. NREL Employees Honored by Industry Associations - News Releases...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Air Conditioning Engineers (ASHRAE) as its New Face of Engineering. The program, part of National Engineers Week, and co-sponsored by ASHRAE, promotes the accomplishments of ...

  6. European Association for Renewable Energy EUROSOLAR | Open Energy...

    Open Energy Info (EERE)

    was founded in 1988 as a nonprofit organization that promotes the replacement of nuclear and fossil energies with Renewable Energy sources. Coordinates: 50.7323, 7.101695...

  7. Iowa Renewable Fuels Association IRFA | Open Energy Information

    Open Energy Info (EERE)

    Product: Fosters the development and growth of renewable fuels industry through education, promotion and infrastructure development in Iowa. Coordinates: 33.831879,...

  8. Effect of metallothionein core promoter region polymorphism on cadmium, zinc and copper levels in autopsy kidney tissues from a Turkish population

    SciTech Connect (OSTI)

    Kayaalti, Zeliha; Mergen, Goerkem; Soeylemezoglu, Tuelin

    2010-06-01

    Metallothioneins (MTs) are metal-binding, low molecular weight proteins and are involved in pathophysiological processes like metabolism of essential metals, metal ion homeostasis and detoxification of heavy metals. Metallothionein expression is induced by various heavy metals especially cadmium, mercury and zinc; MTs suppress toxicity of heavy metals by binding themselves to these metals. The aim of this study was to investigate the association between the - 5 A/G metallothionein 2A (MT2A) single nucleotide polymorphism (SNP) and Cd, Zn and Cu levels in the renal cortex from autopsy cases. MT2A core promoter region - 5 A/G SNP was analyzed by PCR-RFLP method using 114 autopsy kidney tissues and the genotype frequencies of this polymorphism were found as 87.7% homozygote typical (AA), 11.4% heterozygote (AG) and 0.9% homozygote atypical (GG). In order to assess the Cd, Zn and Cu levels in the same autopsy kidney tissues, a dual atomic absorption spectrophotometer system was used and the average levels of Cd, Zn and Cu were measured as 95.54 {+-} 65.58 {mu}g/g, 181.20 {+-} 87.72 {mu}g/g and 17.14 {+-} 16.28 {mu}g/g, respectively. As a result, no statistical association was found between the - 5 A/G SNP in the MT2A gene and the Zn and Cu levels in the renal cortex (p > 0.05), but considerably high accumulation of Cd was monitored for individuals having AG (151.24 {+-} 60.21 {mu}g/g) and GG genotypes (153.09 {mu}g/g) compared with individuals having AA genotype (87.72 {+-} 62.98 {mu}g/g) (p < 0.05). These results show that the core promoter region polymorphism of metallothionein 2A increases the accumulation of Cd in human renal cortex.

  9. Aleutian Pribilof Islands Association - Wind Energy Development

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    In the Aleutian Pribilof Islands Tribal Energy Program Review November 18, 2008 By Bruce Wright Connie Fredenberg Aleutian Pribilof Islands Association "The Birthplace of the Wind" Aleutian Pribilof Islands Association, Inc. * 150 mph gusts * Extreme Turbulence Potential * Corrosive Salt Spray World Class Wind: A Mixed Blessing Aleutian Pribilof Islands Association, Inc. LOGISTICS * Anchorage to Nikolski is 916 air miles for $1,316 rt. * During the fishing season a refundable ticket

  10. DOE Renews Western Governors' Association Cooperative Agreement |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Renews Western Governors' Association Cooperative Agreement DOE Renews Western Governors' Association Cooperative Agreement June 29, 2016 - 5:00pm Addthis Media Contact: Media Contact: Bill Taylor (575) 234-7591 bill.taylor@cbfo.doe.gov Carlsbad, NM - The Department of Energy 's Carlsbad Field Office today renewed the cooperative agreement with the Western Governors' Association (WGA) by extending the project period five years to June 30, 2021. The five-year renewal is

  11. Known Challenges Associated with the Production, Transportation...

    Energy Savers [EERE]

    Usage of Pyrolysis Oil in Residential and Industrial Settings Known Challenges Associated with the Production, Transportation, Storage and Usage of Pyrolysis Oil in Residential and ...

  12. Good Company Associates Inc | Open Energy Information

    Open Energy Info (EERE)

    Zip: 78701 Sector: Efficiency, Renewable Energy Product: Good Company Associates is a business development consulting firm specializing in energy efficiency, renewables, and...

  13. Deputy Associate Under Secretary for Security

    Broader source: Energy.gov [DOE]

    The Office of the Associate Under Secretary for Environment, Health, Safety, and Security (AU) provides corporate leadership and strategic approaches for protecting DOEs workers, the public, the...

  14. Associate Directorate for Environmental Programs Update July...

    Office of Environmental Management (EM)

    July 30, 2014 Associate Directorate for Environmental Programs Update July 30, 2014 Topics: LANL Nitrate Salts Waste Chromium groundwater Remediation Storm Water Field Work PDF...

  15. NC Sustainable Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Jump to: navigation, search Name: NC Sustainable Energy Association Address: PO Box 6465 Place: Raleigh Zip: 27628 Number of Employees: 1-10 Year Founded: 1978 Phone...

  16. International Air Transport Association (IATA) | Open Energy...

    Open Energy Info (EERE)

    Name: International Air Transport Association (IATA) Address: 800 Place Victoria PO Box 113 Place: Montreal, Quebec Phone Number: 1 514 874 0202 Website: www.iata.org...

  17. JP Sercel Associates Inc | Open Energy Information

    Open Energy Info (EERE)

    Inc Jump to: navigation, search Name: JP Sercel Associates Inc Place: Manchester, New Hampshire Zip: 3102 Sector: Solar Product: US-based manufacturer of scribing machinese...

  18. North Associated Power Corporation | Open Energy Information

    Open Energy Info (EERE)

    navigation, search Name: North Associated Power Corporation Place: Huhehaote, Inner Mongolia Autonomous Region, China Zip: 10020 Product: A company generating power for Inner...

  19. Associate Directorate for Environmental Programs Update January...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Associate Directorate for Environmental Programs Update January 28, 2015 Topics: Update on Nitrate Salts Chromium Remediation Project MDA L Soil Vapor Extraction PDF icon ADEP ...

  20. Montana Association of Conservation Districts Webpage | Open...

    Open Energy Info (EERE)

    Districts Webpage Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: Montana Association of Conservation Districts Webpage Abstract Homepage of...

  1. QER - Comment of Irrigation & Electrical Districts Association...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Association of Arizona From: Robert S. Lynch RSLynch@rslynchaty.com Sent: Monday, August 18, 2014 7:26 PM To: QERcomments CC: Kevin Kinsall; James Ogsbury; ...

  2. Valley Electric Association- Solar Water Heating Program

    Office of Energy Efficiency and Renewable Energy (EERE)

    Valley Electric Association (VEA), a nonprofit member owned cooperative, developed the domestic solar water heating program to encourage energy efficiency at the request of the membership. VEA...

  3. Golden Valley Electric Association - Residential Energy Efficiency...

    Broader source: Energy.gov (indexed) [DOE]

    30 Timer Controlling Exterior Vehicle Plug-In Outlet: 20 Switch Controlling Exterior Vehicle Plug-In Outlet: 10 Summary Golden Valley Electric Association's (GVEA) Builder...

  4. Northeast Sustainable Energy Association (Massachusetts) | Open...

    Open Energy Info (EERE)

    (Massachusetts) Jump to: navigation, search Name: Northeast Sustainable Energy Association Address: 50 Miles Street Place: Greenfield, Massachusetts Zip: 01301 Region: Greater...

  5. PROPERTIES OF SEQUENTIAL CHROMOSPHERIC BRIGHTENINGS AND ASSOCIATED...

    Office of Scientific and Technical Information (OSTI)

    We report on the physical properties of solar sequential chromospheric brightenings ... (kernels) of solar flares and associated SCBs using high-resolution Halpha images. ...

  6. Working with Condominium Owners and Associations | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Working with Condominium Owners and Associations Better Buildings Neighborhood Program Multifamily and Low-Income Peer Exchange Call: Working with Condominium Owners and ...

  7. Appalachian Advanced Energy Association | Open Energy Information

    Open Energy Info (EERE)

    search Name: Appalachian Advanced Energy Association Address: 4 E. Hunter St. Place: Logan, Ohio Zip: 43138 Sector: Efficiency, Renewable Energy, Services Phone Number:...

  8. Offshore Infrastructure Associates Inc | Open Energy Information

    Open Energy Info (EERE)

    search Name: Offshore Infrastructure Associates Inc Region: Puerto Rico Sector: Marine and Hydrokinetic Website: http: This company is listed in the Marine and Hydrokinetic...

  9. Known Challenges Associated with the Production, Transportation...

    Energy Savers [EERE]

    Challenges Associated with the Production, Transportation, Storage and Usage of Pyrolysis Oil in Residential and Industrial Settings Technical Information Exchange on Pyrolysis Oil ...

  10. American Evaluation Association Guiding Principles for Evaluators...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Five principles developed by American Evaluation Associ intended to guide professional practice of evaluators & to inform evaluation clients and the general public about principles ...

  11. Consent Order, Brookhaven Science Associates, LLC | Department...

    Energy Savers [EERE]

    Consent Order issued to Brookhaven Science Associates, LLC relating to an electrical shock event that occurred at the Brookhaven National Laboratory. On November 23,...

  12. Acid-functionalized polyolefin materials and their use in acid-promoted chemical reactions

    DOE Patents [OSTI]

    Oyola, Yatsandra; Tian, Chengcheng; Bauer, John Christopher; Dai, Sheng

    2016-06-07

    An acid-functionalized polyolefin material that can be used as an acid catalyst in a wide range of acid-promoted chemical reactions, wherein the acid-functionalized polyolefin material includes a polyolefin backbone on which acid groups are appended. Also described is a method for the preparation of the acid catalyst in which a precursor polyolefin is subjected to ionizing radiation (e.g., electron beam irradiation) of sufficient power and the irradiated precursor polyolefin reacted with at least one vinyl monomer having an acid group thereon. Further described is a method for conducting an acid-promoted chemical reaction, wherein an acid-reactive organic precursor is contacted in liquid form with a solid heterogeneous acid catalyst comprising a polyolefin backbone of at least 1 micron in one dimension and having carboxylic acid groups and either sulfonic acid or phosphoric acid groups appended thereto.

  13. Manipulation of lignin composition in plants using a tissue-specific promoter

    DOE Patents [OSTI]

    Chapple, Clinton C. S.

    2003-08-26

    The present invention relates to methods and materials in the field of molecular biology, the manipulation of the phenylpropanoid pathway and the regulation of proteins synthesis through plant genetic engineering. More particularly, the invention relates to the introduction of a foreign nucleotide sequence into a plant genome, wherein the introduction of the nucleotide sequence effects an increase in the syringyl content of the plant's lignin. In one specific aspect, the invention relates to methods for modifying the plant lignin composition in a plant cell by the introduction there into of a foreign nucleotide sequence comprising at issue specific plant promoter sequence and a sequence encoding an active ferulate-5-hydroxylase (F5H) enzyme. Plant transformants harboring an inventive promoter-F5H construct demonstrate increased levels of syringyl monomer residues in their lignin, rendering the polymer more readily delignified and, thereby, rendering the plant more readily pulped or digested.

  14. Technology certification and technology acceptance: Promoting interstate cooperation and market development for innovative technologies

    SciTech Connect (OSTI)

    Brockbank, B.R.

    1995-03-01

    In the past two years, public and private efforts to promote development and deployment of innovative environmental technologies have shifted from the analysis of barriers to the implementation of a variety of initiatives aimed at surmounting those barriers. Particular attention has been directed at (1) streamlining fragmented technology acceptance processes within and among the states, and (2) alleviating disincentives, created by inadequate or unverified technology cost and performance data, for users and regulators to choose innovative technologies. Market fragmentation currently imposes significant cost burdens on technology developers and inhibits the investment of private capital in environmental technology companies. Among the responses to these problems are state and federal technology certification/validation programs, efforts to standardize cost/performance data reporting, and initiatives aimed at promoting interstate cooperation in technology testing and evaluation. This paper reviews the current status of these initiatives, identifies critical challenges to their success, and recommends strategies for addressing those challenges.

  15. Federal policies to promote the widespread utilization of photovoltaic systems. Supplement: review and critique

    SciTech Connect (OSTI)

    Smith, J.L.

    1980-04-15

    This document is intended as a supplement to the two-volume report entitled Federal Policies to Promote the Widespread Utilization of Photovoltaic Systems that was submitted to Congress by the Department of Energy in February and April of 1980. This supplement contains review comments prepared by knowledgeable experts who reviewed early drafts of the Congressional report. Responses to the review comments by the Jet Propulsion Laboratory, preparer of the Congressional report, are also included in this supplement. The Congressional report, mandated in the Solar Photovoltaic Energy Research, Development, and Demonstration Act of 1978 (P.L. 95-590), discusses various issues related to promoting the deployment of photovoltaic systems through the Federal Photovoltaic Program. Various program strategies and funding levels are examined.

  16. Cracking of n-butane catalyzed by iron- and maganese-promoted sulfated zirconia

    SciTech Connect (OSTI)

    Cheung, T.K.; d`Itri, J.L.; Gates, B.C.

    1995-05-01

    Fe- and Mn-promoted sulfated zirconia was used to catalyze the conversion of n-butane at atmospheric pressure and n-butane partial pressures in the range of 0.0025-0.01 atm. At temperatures <225{degrees}C, the significant reactions were isomerization and disproportionation; in the range of 225-300{degrees}C, these reactions were accompanied by cracking, and at temperatures >350{degrees}C, cracking and isomerization occurred. Catalyst deactivation, resulting at least in part from coke formation, was rapid. The primary cracking products were methane, ethane, ethylene, and propylene. The observation of these products along with an ethane/ethylene molar ratio of nearly 1 at 450{degrees}C is consistent with cracking occurring, at least in part, by the Haag-Dessau mechanism, whereby the strongly acidic catalyst protonates n-butane to give carbonium ions. The rate of methane formation from n-butane cracking catalyzed by Fe- and Mn-promoted sulfated zirconia at 450{degrees}C was about 3 x 10{sup {minus}9}mol/(g of catalyst {center_dot}s). This comparison suggests that the catalytic activity of the promoted sulfated zirconia at 450{degrees}C is about the same as that of the zeolite, although its activity for n-butane isomerization and disproportionation at temperatures <100{degrees}C is orders of magnitude greater than those of zeolites. Thus the indication of superacidity of the promoted sulfated zirconia does not extend to high temperatures. The results raise questions about the nature of the presumed superacidity: perhaps the low-temperature reactions may involve catalyst functions other than the acidic function responsible for high-temperature cracking reactions or perhaps superacidic sites may be very rapidly poisoned at cracking temperatures. 14 refs., 8 figs., 3 tabs.

  17. Redox Mediators that Promote Three-Dimensional Growth of Li2S on Carbon

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Current Collectors in Lithium-Sulfur Batteries - Joint Center for Energy Storage Research 21, 2015, Research Highlights Redox Mediators that Promote Three-Dimensional Growth of Li2S on Carbon Current Collectors in Lithium-Sulfur Batteries Controlling the electrodeposition of Li2S onto C using a redox mediator, BPI. With BPI, sulfur utilization improves in Li-S cells due to remote reduction of polysulfides to Li2S. Scientific Achievement Developed, from computation and experiment, redox

  18. CATALYTIC PROMOTION OF THE ADSORPTION OF VANADIUM ON AN ANIONIC EXCHANGE RESIN

    DOE Patents [OSTI]

    Bailes, R.H.; Ellis, D.A.

    1958-08-26

    An improvement in the process for the recovery of vanadium from acidic phosphatic solutions is presented. In this process the vanadium is first oxidized to the pentavaleat state, and is then separated by contacting such solutions with an anion exchange resin whereby adsorption of the complexed pentavalent vanadium is effected. The improvement lies in the fact that adsorp tion of the vanadium complex by the anion exchange resin is promoted and improved by providing fiuoride ions in solution to be contacted.

  19. A Model for the Nation: Promoting Education and Innovation in Vermont's

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Electricity Sector Model for the Nation: Promoting Education and Innovation in Vermont's Electricity Sector - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid

  20. Global Assessment of Hydrogen Technologies – Task 6 Report Promoting a Southeast Hydrogen Consortium

    SciTech Connect (OSTI)

    Fouad, Fouad H.; Peters, Robert W.; Sisiopiku, Virginia P.; Sullivan Andrew J.

    2007-12-01

    The purpose of this project task was to establish a technical consortium to promote the deployment of hydrogen technologies and infrastructure in the Southeast. The goal was to partner with fuel cell manufacturers, hydrogen fuel infrastructure providers, electric utilities, energy service companies, research institutions, and user groups to improve education and awareness of hydrogen technologies in an area that is lagging behind other parts of the country in terms of vehicle and infrastructure demonstrations and deployments. This report documents that effort.

  1. Energy Secretary Abraham Promotes Smart Energy Use in Tour of National

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Renewable Energy Laboratory - News Releases | NREL Energy Secretary Abraham Promotes Smart Energy Use in Tour of National Renewable Energy Laboratory July 1, 2003 Golden, CO. - Touring the U.S. Department of Energy's National Renewable Energy Laboratory today, Secretary of Energy Spencer Abraham encouraged Americans to use energy efficient technologies and practices in their homes and businesses in the wake of rising natural gas prices this year. Demand for natural gas is expected to rise by

  2. PROMOTING THE ECONOMY AND CULTURAL WELFARE OF NOGALES AND SANTA CRUZ COUNTY

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Kino Park Nogales, Arizona 85621 Phone Number: (520) 287-3685 Fax Number: (520) 287-3687 Email: info@thenogaleschamber.org Website: www.thenogaleschamber.org PROMOTING THE ECONOMY AND CULTURAL WELFARE OF NOGALES AND SANTA CRUZ COUNTY June 20 th , 2016 Christopher Lawrence United States Department of Energy 1000 Independence Avenue, SW Washington, DC 20585 Re: Nogales Interconnection Project Presidential Permit Application Dear Mr. Lawrence: We are writing this letter in urgency for the U.S.

  3. Active hopper for promoting flow of bulk granular or powdered solids

    DOE Patents [OSTI]

    Saunders, Timothy; Brady, John D.

    2013-04-02

    An apparatus that promotes the flow of materials has a body having an inner shape for holding the materials, a wall having a shape that approximates a portion of the inner shape of the body, and a vibrator attached to the wall. The wall may be disposed vertically within the body close to the body's inner shape. The vibrator transfers vibrations to the wall to agitate the material and encourage material flow.

  4. Development of a gas-promoted oil agglomeration process: Air-promoted oil agglomeration of moderately hydrophobic coals. 2: Effect of air dosage in a model mixing system

    SciTech Connect (OSTI)

    Drzymala, J.; Wheelock, T.D.

    1996-07-01

    In a selective oil agglomeration process for cleaning coal, fine-size particles are suspended in water and treated with a water-immiscible hydrocarbon which can range from pentane to heavy fuel oil. Vigorous agitation is applied to disperse the oil and to produce frequent contacts between oil-coated particles. In Part 1 of this series of papers, it was shown that a definite amount of air had to be present in a laboratory mixing unit which produced a moderate shear rate in order to form compact, spherical agglomerates in an aqueous suspension of moderately hydrophobic coal using heptane or hexadecane as an agglomerate. In this paper, the effects of different amounts of air including dissolved air are discussed. The results indicate that a small amount of air will trigger the process of agglomeration, and even the air dissolved in water under equilibrium conditions at room temperature and pressure is sufficient to promote agglomeration provided it is released from solution.

  5. MicroRNA-27a promotes myoblast proliferation by targeting myostatin

    SciTech Connect (OSTI)

    Huang, Zhiqing; Chen, Xiaoling; Yu, Bing; He, Jun; Chen, Daiwen

    2012-06-29

    Highlights: Black-Right-Pointing-Pointer We identified a myogenic role for miR-27a and a new target, myostatin. Black-Right-Pointing-Pointer The miR-27a was confirmed to target myostatin 3 Prime UTR. Black-Right-Pointing-Pointer miR-27a is upregulated and myostatin is downregulated during myoblast proliferation. Black-Right-Pointing-Pointer miR-27a promotes myoblast proliferation by reducing the expression of myostatin. -- Abstract: MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that play critical roles in skeletal muscle development as well as in regulation of muscle cell proliferation and differentiation. However, the role of miRNAs in myoblast proliferation remains poorly understood. Here we found that the expression of miR-27a was increased during proliferation of C2C12 myoblasts. Moreover, overexpression of miR-27a in C2C12 cells promoted myoblast proliferation by reducing the expression of myostatin, a critical inhibitor of skeletal myogenesis. In addition, the miR-27a was confirmed to target myostatin 3 Prime UTR by a luciferase reporter analysis. Together, these results suggest that miR-27a promotes myoblast proliferation through targeting myostatin.

  6. Pioglitazone promotes preadipocyte proliferation by downregulating p16{sup Ink4a}

    SciTech Connect (OSTI)

    Hasan, Arif U.; Ohmori, Koji; Hashimoto, Takeshi; Kamitori, Kazuyo; Hirata, Yuko; Ishihara, Yasuhiro; Okamoto, Naoko; Noma, Takahisa; Kosaka, Hiroaki; Tokuda, Masaaki; Kohno, Masakazu

    2011-07-29

    Highlights: {yields} Mechanisms for preadipocyte hyperplasia by pioglitazone, a PPAR{gamma} agonist, are shown. {yields} Pioglitazone promotes cell-cycle of 3T3-L1 preadipocytes and increases their number. {yields} Pioglitazone downregulates a cyclin dependent kinase inhibitor, p16{sup Ink4a}. {yields} PPAR{gamma} transrepresses p16{sup Ink4a} gene in preadipocytes, which pioglitazone enhances. -- Abstract: Pioglitazone, a synthetic ligand of peroxisome proliferator-activated receptor (PPAR){gamma}, causes preadipocyte proliferation through a mechanism which still remains elusive. Here, to address the mechanism, we investigated the effects of PPAR{gamma} and pioglitazone on the kinetics of cyclin-dependent kinase inhibitors, especially with p16{sup Ink4a} (p16) centered, by employing 3T3-L1 preadipocytes. Pioglitazone promoted preadipocyte proliferation by increasing S and G{sub 2}/M cell-cycle entry, which was accompanied by decreased p16 mRNA expression. PPAR{gamma} overexpression along with the luciferase reporter assay confirmed that PPAR{gamma} was crucial for the downregulation of p16 mRNA transcription, and that the action was augmented by pioglitazone. Thus, pioglitazone exerted cell-cycle dependent promoting effect on preadipocyte proliferation, of which mechanisms include p16-downregulation through PPAR{gamma}.

  7. South Asia Water Resources Workshop: An effort to promote water quality data sharing in South Asia

    SciTech Connect (OSTI)

    RAJEN,GAURAV; BIRINGER,KENT L.; BETSILL,J. DAVID

    2000-04-01

    To promote cooperation in South Asia on environmental research, an international working group comprised of participants from Bangladesh, India, Nepal, Pakistan, Sri Lanka, and the US convened at the Soaltee Hotel in Kathmandu, Nepal, September 12 to 14, 1999. The workshop was sponsored in part by the Cooperative Monitoring Center (CMC) at Sandia National Laboratories in Albuquerque, New Mexico, through funding provided by the Department of Energy (DOE) Office of Nonproliferation and National Security. The CMC promotes collaborations among scientists and researchers in regions throughout the world as a means of achieving common regional security objectives. In the long term, the workshop organizers and participants are interested in the significance of regional information sharing as a means to build confidence and reduce conflict. The intermediate interests of the group focus on activities that might eventually foster regional management of some aspects of water resources utilization. The immediate purpose of the workshop was to begin the implementation phase of a project to collect and share water quality information at a number of river and coastal estuary locations throughout the region. The workshop participants achieved four objectives: (1) gaining a better understanding of the partner organizations involved; (2) garnering the support of existing regional organizations promoting environmental cooperation in South Asia; (3) identifying sites within the region at which data is to be collected; and (4) instituting a data and information collection and sharing process.

  8. Extracorporeal membrane oxygenation promotes long chain fatty acid oxidation in the immature swine heart in vivo

    SciTech Connect (OSTI)

    Kajimoto, Masaki; O'Kelly-Priddy, Colleen M.; Ledee, Dolena R.; Xu, Chun; Isern, Nancy G.; Olson, Aaron; Portman, Michael A.

    2013-09-01

    Extracorporeal membrane oxygenation (ECMO) supports infants and children with severe cardiopulmonary compromise. Nutritional support for these children includes provision of medium- and long-chain fatty acids (FAs). However, ECMO induces a stress response, which could limit the capacity for FA oxidation. Metabolic impairment could induce new or exacerbate existing myocardial dysfunction. Using a clinically relevant piglet model, we tested the hypothesis that ECMO maintains the myocardial capacity for FA oxidation and preserves myocardial energy state. Provision of 13-Carbon labeled medium-chain FA (octanoate), longchain free FAs (LCFAs), and lactate into systemic circulation showed that ECMO promoted relative increases in myocardial LCFA oxidation while inhibiting lactate oxidation. Loading of these labeled substrates at high dose into the left coronary artery demonstrated metabolic flexibility as the heart preferentially oxidized octanoate. ECMO preserved this octanoate metabolic response, but also promoted LCFA oxidation and inhibited lactate utilization. Rapid upregulation of pyruvate dehydrogenase kinase-4 (PDK4) protein appeared to participate in this metabolic shift during ECMO. ECMO also increased relative flux from lactate to alanine further supporting the role for pyruvate dehydrogenase inhibition by PDK4. High dose substrate loading during ECMO also elevated the myocardial energy state indexed by phosphocreatine to ATP ratio. ECMO promotes LCFA oxidation in immature hearts, while maintaining myocardial energy state. These data support the appropriateness of FA provision during ECMO support for the immature heart.

  9. PDGFBB promotes PDGFR{alpha}-positive cell migration into artificial bone in vivo

    SciTech Connect (OSTI)

    Yoshida, Shigeyuki; Center for Human Metabolomic Systems Biology, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582 ; Iwasaki, Ryotaro; Kawana, Hiromasa; Miyauchi, Yoshiteru; Department of Orthopedic Surgery, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582; Department of Integrated Bone Metabolism and Immunology, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582 ; Hoshi, Hiroko; Miyamoto, Hiroya; Mori, Tomoaki; Department of Orthopedic Surgery, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582 ; Kanagawa, Hiroya; Katsuyama, Eri; Fujie, Atsuhiro; Department of Orthopedic Surgery, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582 ; Hao, Wu; and others

    2012-05-18

    Highlights: Black-Right-Pointing-Pointer We examined effects of PDGFBB in PDGFR{alpha} positive cell migration in artificial bones. Black-Right-Pointing-Pointer PDGFBB was not expressed in osteoblastic cells but was expressed in peripheral blood cells. Black-Right-Pointing-Pointer PDGFBB promoted PDGFR{alpha} positive cell migration into artificial bones but not osteoblast proliferation. Black-Right-Pointing-Pointer PDGFBB did not inhibit osteoblastogenesis. -- Abstract: Bone defects caused by traumatic bone loss or tumor dissection are now treated with auto- or allo-bone graft, and also occasionally by artificial bone transplantation, particularly in the case of large bone defects. However, artificial bones often exhibit poor affinity to host bones followed by bony union failure. Thus therapies combining artificial bones with growth factors have been sought. Here we report that platelet derived growth factor bb (PDGFBB) promotes a significant increase in migration of PDGF receptor {alpha} (PDGFR{alpha})-positive mesenchymal stem cells/pre-osteoblastic cells into artificial bone in vivo. Growth factors such as transforming growth factor beta (TGF{beta}) and hepatocyte growth factor (HGF) reportedly inhibit osteoblast differentiation; however, PDGFBB did not exhibit such inhibitory effects and in fact stimulated osteoblast differentiation in vitro, suggesting that combining artificial bones with PDGFBB treatment could promote host cell migration into artificial bones without inhibiting osteoblastogenesis.

  10. Jeff Griffin, Ph. D. Associate Laboratory Director

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Jay Tilden Associate Administrator and Deputy Under Secretary for Counterterrorism and Counterproliferation Our Leadership Since April 2016, Mr. Tilden has served as the Associate Administrator for Counterterrorism and Counterproliferation with responsibility in preparing for, responding to, and successfully resolving nuclear and radiological accidents and incidents worldwide. The mission scope includes providing expertise, technically informed policy recommendations required to advance U.S.

  11. 2009 Voluntary Protection Programs Participants' Association (VPPPA)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Presentaton: Employee Led Safety Committees | Department of Energy Presentaton: Employee Led Safety Committees 2009 Voluntary Protection Programs Participants' Association (VPPPA) Presentaton: Employee Led Safety Committees 2009 Voluntary Protection Programs Participants' Association (VPPPA) Presentaton: Employee Led Safety Committees Employee Led Safety Committees (302.7 KB) More Documents & Publications VPPPA Presentation: TEAM 2009 Voluntary Protection Programs Participants'

  12. International Ethanol Trade Association IETHA | Open Energy Informatio...

    Open Energy Info (EERE)

    Trade Association IETHA Jump to: navigation, search Name: International Ethanol Trade Association (IETHA) Place: Sao Paulo, Sao Paulo, Brazil Product: Association of 48 globally...

  13. Colorado Solar Energy Industries Association | Open Energy Information

    Open Energy Info (EERE)

    Industries Association Jump to: navigation, search Logo: Colorado Solar Energy Industries Association Name: Colorado Solar Energy Industries Association Address: 841 Front St....

  14. International Solid Waste Association (ISWA) | Open Energy Information

    Open Energy Info (EERE)

    Solid Waste Association (ISWA) Jump to: navigation, search Name: International Solid Waste Association (ISWA) Address: ISWA - International Solid Waste Association General...

  15. Adrian Energy Associates LLC Biomass Facility | Open Energy Informatio...

    Open Energy Info (EERE)

    Adrian Energy Associates LLC Biomass Facility Jump to: navigation, search Name Adrian Energy Associates LLC Biomass Facility Facility Adrian Energy Associates LLC Sector Biomass...

  16. Spanish Wind Energy Association AEE | Open Energy Information

    Open Energy Info (EERE)

    Wind Energy Association AEE Jump to: navigation, search Name: Spanish Wind Energy Association (AEE) Place: Madrid, Spain Zip: 28006 Sector: Wind energy Product: Spain's association...

  17. Texas Renewable Energy Industries Association | Open Energy Informatio...

    Open Energy Info (EERE)

    Renewable Energy Industries Association Jump to: navigation, search Logo: Texas Renewable Energy Industries Association Name: Texas Renewable Energy Industries Association Address:...

  18. Naknek Electric Association | Open Energy Information

    Open Energy Info (EERE)

    Naknek Electric Association Jump to: navigation, search Name: Naknek Electric Assn, Inc Place: Alaska Phone Number: 1 (907) 246-4261 Website: www.nea.coop Outage Hotline: 1 (907)...

  19. Aleutian Pribilof Islands Association- 2005 Project

    Broader source: Energy.gov [DOE]

    The Aleutian Pribilof Islands Association (APIA) was chartered as a nonprofit organization in 1976 and is a federally recognized tribal organization of the Aleut people. APIA will conduct an...

  20. Deb Covey, Associate Director for Sponsored Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    gov Deb Covey, Associate Director for Sponsored Research 311 TASF Ames, IA, 50011 covey@ameslab.gov 515-294-1048 Ames Laboratory, a U.S. Department of Energy national laboratory...

  1. Costs Associated With Propane Vehicle Fueling Infrastructure

    SciTech Connect (OSTI)

    Smith, M.; Gonzales, J.

    2014-08-01

    This document is designed to help fleets understand the cost factors associated with propane vehicle fueling infrastructure. It provides an overview of the equipment and processes necessary to develop a propane fueling station and offers estimated cost ranges.

  2. Costs Associated With Propane Vehicle Fueling Infrastructure

    SciTech Connect (OSTI)

    Smith, M.; Gonzales, J.

    2014-08-05

    This document is designed to help fleets understand the cost factors associated with propane vehicle fueling infrastructure. It provides an overview of the equipment and processes necessary to develop a propane fueling station and offers estimated cost ranges.

  3. Casimir Energy Associated With Fractional Derivative Field

    SciTech Connect (OSTI)

    Lim, S. C.

    2007-04-28

    Casimir energy associated with fractional derivative scalar massless field at zero and positive temperature can be obtained using the regularization based on generalized Riemann zeta function of Epstein-Hurwitz type.

  4. QER- Comment of National Propane Gas Association

    Office of Energy Efficiency and Renewable Energy (EERE)

    Ladies and Gentlemen: Please find attached the QER comments of the National Propane Gas Association. Please feel to contact us if we can provide further information. Thank you for your attention to our submission.

  5. 2014 Beryllium-Associated Worker Registry Summary

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Table of Contents Beryllium-Associated Worker Registry Summary............................................................................................................................... 1 Location of 27 Reporting Organizations Currently Submitting Data to BAWR .............................................................................. 5 27 Reporting Organizations Currently Submitting Data to BAWR

  6. American Evaluation Association Guiding Principles for Evaluators

    SciTech Connect (OSTI)

    None

    2009-01-18

    Five principles developed by American Evaluation Associ intended to guide professional practice of evaluators & to inform evaluation clients and the general public about principles they can expect to be upheld by professional evaluators.

  7. Association of Home Appliance Manufacturers Comment

    Broader source: Energy.gov [DOE]

    The Association of Home Appliance Manufacturers (AHAM) respectfully submits the following comments to the Department of Energy (DOE) on its Regulatory Burden RFI, 79 Fed. Reg. 37963 (July 3, 2014).

  8. National Electrical Manufacturers Association Comment | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Comment National Electrical Manufacturers Association Comment The National Electrical Manufacturers Association (NEMA) appreciates the opportunity to provide the attached comments on the Request for Information to Reduce Regulatory Burden as announced in the U.S. Federal Register Vol. 80, No. 127, beginning on page 38019. Regulatory Burden RFI NEMA Comments regarding small motor regulations 17July2015 v5_1 (174.95 KB) More Documents & Publications Ex Parte Communications in

  9. Associative memory in phasing neuron networks

    SciTech Connect (OSTI)

    Nair, Niketh S; Bochove, Erik J.; Braiman, Yehuda

    2014-01-01

    We studied pattern formation in a network of coupled Hindmarsh-Rose model neurons and introduced a new model for associative memory retrieval using networks of Kuramoto oscillators. Hindmarsh-Rose Neural Networks can exhibit a rich set of collective dynamics that can be controlled by their connectivity. Specifically, we showed an instance of Hebb's rule where spiking was correlated with network topology. Based on this, we presented a simple model of associative memory in coupled phase oscillators.

  10. Bellows Joins NREL Staff as Associate Director

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bellows Joins NREL Staff as Associate Director For more information contact: e:mail: Public Affairs Golden, Colo., April 21, 1997 -- Jerry L. Bellows recently joined the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) as Associate Director. Bellows previously served as Area Manager at DOE's Rocky Flats Plant, NREL, and Brookhaven and Los Alamos National Laboratories. He also was Director of DOE's Office of Contract Reform in the Office of the Deputy Secretary in

  11. National Postdoctoral Association | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    National Postdoctoral Association The National Postdoctoral Association (NPA) is a member-driven organization that provides a unique, national voice for postdoctoral scholars. Since 2003, we have taken on the ambitious agenda to enhance the quality of the postdoctoral experience in the U.S. We have assumed a leadership role in addressing the many issues confronting the postdoctoral community that are national in scope and requiring action beyond the local level. Read more. Argonne National Lab

  12. Journal of the National Grants Management Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    7 No. 1 - 2009 TABLE OF CONTENTS of the NATIONAL GRANTS MANAGEMENT ASSOCIATION J OURNAL National Grants Management Association 11654 Plaza America Drive, #609 Reston, VA 20190-4700 USA Phone: 703.648.9023 * Fax: 703.648.9024 www.ngma.org Implementing a Risk-Based Strategy for Monitoring Federal Award Portfolios Mary Santonastasso, Erika Rissi and Michael Austin Using Automated Logic Models to Enhance Grants Management Barry Nazar, Frederick Richmond, Manuel Valentin and Barbara Dorf Transforming

  13. Los Alamos' Terwilliger named American Crystallographic Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    president Terwilliger named American Crystallographic Association president Los Alamos' Terwilliger named American Crystallographic Association president The ACA is the home to more than 1,000 scientists around the country who use X-rays, neutrons and electrons to see the shapes of molecules. April 27, 2016 Tom Terwilliger Tom Terwilliger Contact Nick Njegomir Communications Office (505) 665-9394 Email "This is an exciting time for crystallography because there are new ways to look at

  14. Working with CMI: Associates | Critical Materials Institute

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Associates CMI Associates work with CMI researchers to define a scope of work, budget and timeline for the work. Once internally approved, the entity must execute either a CRADA or SPP with Ames or another Team national laboratory before work may begin. For more information: CMIaffiliates@ameslab.gov CMI Affiliates Membership, The Ames Laboratory, 306 TASF, 2408 Pammel Drive, Ames, IA, 50011-1015

  15. Histone H4 Lys 20 methyltransferase SET8 promotes androgen receptor-mediated transcription activation in prostate cancer

    SciTech Connect (OSTI)

    Yao, Lushuai; Li, Yanyan; Du, Fengxia; Han, Xiao; Li, Xiaohua; Niu, Yuanjie; Ren, Shancheng; Sun, Yingli

    2014-07-18

    Highlights: • Dihydrotestosterone stimulates H4K20me1 enrichment at the PSA promoter. • SET8 promotes AR-mediated transcription activation. • SET8 interacts with AR and promotes cell proliferation. - Abstract: Histone methylation status in different lysine residues has an important role in transcription regulation. The effect of H4K20 monomethylation (H4K20me1) on androgen receptor (AR)-mediated gene transcription remains unclear. Here we show that AR agonist stimulates the enrichment of H4K20me1 and SET8 at the promoter of AR target gene PSA in an AR dependent manner. Furthermore, SET8 is crucial for the transcription activation of PSA. Co-immunoprecipitation analyses demonstrate that SET8 interacts with AR. Therefore, we conclude that SET8 is involved in AR-mediated transcription activation, possibly through its interaction with AR and H4K20me1 modification.

  16. Method for promoting specific alignment of short oligonucleotides on nucleic acids

    DOE Patents [OSTI]

    Studier, F. William; Kieleczawa, Jan; Dunn, John J.

    1996-01-01

    Disclosed is a method for promoting specific alignment of short oligonucleotides on a nucleic acid polymer. The nucleic acid polymer is incubated in a solution containing a single-stranded DNA-binding protein and a plurality of oligonucleotides which are perfectly complementary to distinct but adjacent regions of a predetermined contiguous nucleotide sequence in the nucleic acid polymer. The plurality of oligonucleotides anneal to the nucleic acid polymer to form a contiguous region of double stranded nucleic acid. Specific application of the methods disclosed include priming DNA synthesis and template-directed ligation.

  17. Magnetohydrodynamic pump with a system for promoting flow of fluid in one direction

    DOE Patents [OSTI]

    Lemoff, Asuncion V.; Lee, Abraham P.

    2010-07-13

    A magnetohydrodynamic pump for pumping a fluid. The pump includes a microfluidic channel for channeling the fluid, a MHD electrode/magnet system operatively connected to the microfluidic channel, and a system for promoting flow of the fluid in one direction in the microfluidic channel. The pump has uses in the medical and biotechnology industries for blood-cell-separation equipment, biochemical assays, chemical synthesis, genetic analysis, drug screening, an array of antigen-antibody reactions, combinatorial chemistry, drug testing, medical and biological diagnostics, and combinatorial chemistry. The pump also has uses in electrochromatography, surface micromachining, laser ablation, inkjet printers, and mechanical micromilling.

  18. Hong Qin promoted to executive dean at the University of Science and

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technology of China | Princeton Plasma Physics Lab Hong Qin promoted to executive dean at the University of Science and Technology of China By John Greenwald December 19, 2014 Tweet Widget Google Plus One Share on Facebook Hong Qin (Photo by Elle Starkman/PPPL Office of Communications) Hong Qin Hong Qin bestrides the globe as a leading scientist and educator. For the past four years he has shuttled between PPPL and a teaching post at the University of Science and Technology of China (USTC),

  19. Hong Qin promoted to executive dean at the University of Science and

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technology of China | Princeton Plasma Physics Lab Hong Qin promoted to executive dean at the University of Science and Technology of China By John Greenwald December 19, 2014 Tweet Widget Google Plus One Share on Facebook Hong Qin (Photo by Elle Starkman/PPPL Office of Communications) Hong Qin Hong Qin bestrides the globe as a leading scientist and educator. For the past four years he has shuttled between PPPL and a teaching post at the University of Science and Technology of China (USTC),

  20. Asian success stories in promoting energy efficiency in industry and building

    SciTech Connect (OSTI)

    Yang, Ming

    1996-12-31

    This article describes the program of the International Institute for Energy Conservation (IIEC), which has offices in Washington, Bangkok, Santiago, and London, in addition to staff in a number of other countries. The mission of this private organization is to promote the efficient use of energy as a tool for sustainable development by supporting the development of policies, technologies, and practices. Its focus is on energy efficiency, transportation systems, and renewable energy sources. Examples of specific program activities in Thailand, China, Philippines, Malaysia, Indonesia and Singapore are discussed.

  1. Research and Technical Associates | Photosynthetic Antenna Research Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Technical Associates Research and Technical Associates Sneha Chatterjee Post-Masters Read more about Sneha Chatterjee Alexsander Roszak Research Associate Read more about Alexsander Roszak Evgeny Ostroumov Associate Professional Specialist Read more about Evgeny Ostroumov Carrie Goodson Research Technician Read more about Carrie Goodson Maitrayee Bhattacharyya Senior Research Associate Read more about Maitrayee Bhattacharyya Gaozhong Shen Senior Research Associate Read more about Gaozhong

  2. MiR-125a TNF receptor-associated factor 6 to inhibit osteoclastogenesis

    SciTech Connect (OSTI)

    Guo, Li-Juan; Liao, Lan; Yang, Li; Li, Yu; Jiang, Tie-Jian

    2014-02-15

    MicroRNAs (miRNAs) play important roles in osteoclastogenesis and bone resorption. In the present study, we found that miR-125a was dramatically down-regulated during macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor-κB ligand (RANKL) induced osteoclastogenesis of circulating CD14+ peripheral blood mononuclear cells (PBMCs). Overexpression of miR-125a in CD14+ PBMCs inhibited osteoclastogenesis, while inhibition of miR-125a promoted osteoclastogenesis. TNF receptor-associated factor 6 (TRAF6), a transduction factor for RANKL/RANK/NFATc1 signal, was confirmed to be a target of miR-125a. EMSA and ChIP assays confirmed that NFATc1 bound to the promoter of the miR-125a. Overexpression of NFATc1 inhibited miR-125a transcription, and block of NFATc1 expression attenuated RANKL-regulated miR-125a transcription. Here, we reported that miR-125a played a biological function in osteoclastogenesis through a novel TRAF6/ NFATc1/miR-125a regulatory feedback loop. It suggests that regulation of miR-125a expression may be a potential strategy for ameliorating metabolic disease. - Highlights: • MiR-125a was significantly down-regulated in osteoclastogenesis of CD14+ PBMCs. • MiR-125a inhibited osteoclast differentiation by targeting TRAF6. • NFATc1 inhibited miR-125a transciption by binding to the promoter of miR-125a. • TRAF6/NFATc1 and miR-125a form a regulatory feedback loop in osteoclastogenesis.

  3. Recent federal initiatives to promote unconventional gas: High octane delivery of just hot air?

    SciTech Connect (OSTI)

    Griff, M.T.

    1995-10-01

    This paper provides an overview of recent initiatives of the United States which promote greater use of natural gas and unconventional gas as one part of this nations`s larger response to the global warming threat. Measurable increases in greenhouse gas concentrations since the beginning of the industrial revolution have led to the belief in the existence of a global warming problem. The international community has responded to the global warming threat with the United Nations Framework Convention on Climate Change which is directed toward the stabilization of greenhouse gases in the atmosphere. The Climate Change Action Plan is the Clinton Administration`s detailed response to the global warming threat. It is designed to return United States emissions of greenhouse gases to their 1990 levels by the year 2000. The Action Plan targets all greenhouse gases and emphasizes energy efficiency. Significant regulatory reformation designed to increase the efficiency of the natural gas industry has already occurred and will be continued. Recovery of methane emissions from landfills will be encouraged through indentification of suitable sites and use of existing technology and development of new technology. Recovery of methane from coal mining operations will be promoted by targeting 50 of the gassiest mines in the United States. Even if the Action Plan is fully implemented. legitimate questions arise as to whether its goals will be achieved as a result of funding shortfalls.

  4. Ethanol Steam Reforming on Co/CeO2: The Effect of ZnO Promoter

    SciTech Connect (OSTI)

    Davidson, Stephen; Sun, Junming; Wang, Yong

    2013-12-02

    A series of ZnO promoted Co/CeO2 catalysts were synthesized and characterized using XRD, TEM, H2-TPR, CO chemisorption, O2-TPO, IR-Py, and CO2-TPD. The effects of ZnO on the catalytic performances of Co/CeO2 were studied in ethanol steam reforming. It was found that the addition of ZnO facilitated the oxidation of Co0 via enhanced oxygen mobility of the CeO2 support which decreased the activity of Co/CeO2 in C–C bond cleavage of ethanol. 3 wt% ZnO promoted Co/CeO2 exhibited minimum CO and CH4 selectivity and maximum CO2 selectivity. This resulted from the combined effects of the following factors with increasing ZnO loading: (1) enhanced oxygen mobility of CeO2 facilitated the oxidation of CHx and CO to form CO2; (2) increased ZnO coverage on CeO2 surface reduced the interaction between CHx/CO and Co/CeO2; and (3) suppressed CO adsorption on Co0 reduced CO oxidation rate to form CO2. In addition, the addition of ZnO also modified the surface acidity and basicity of CeO2, which consequently affected the C2–C4 product distributions.

  5. Low severity coal liquefaction promoted by cyclic olefins. Quarterly report, April--June 1992

    SciTech Connect (OSTI)

    Curtis, C.W.

    1992-07-27

    Low severity coal liquefaction allows for solubilization of coal with reduced gas make. These lower severity conditions may result in some selective bond rupture. Promotion of coal solubilization through hydrogen transfer using highly active and effective hydrogen donors is the objective of this study. The highly effective donors being tested are cyclic olefins. Representative cyclic olefins are isotetralin, which is 1,4,5,8-tetrahydronaphthalene, and 1,4,5,8,9,10-hexahydroanthracene. These compounds are hydroaromatics without aromatic rings and have been shown to be highly effective donors. The objective of the work performed in this study during this quarter was to evaluate reaction parameters for low severity liquefaction reactions using the cyclic olefin, hexahydroanthracene, and the aromatic, anthracene. These model compounds were reacted under a variety of conditions to evaluate their reactivity without coal. The reactions were performed under both thermal and catalytic conditions. Finely divided catalysts from different molybdenum precursors were used to determine their activity in promoting hydrogenation and hydrogen transfer at low severity conditions. The catalysts used were Molyvan L, sulfurized oxymolybdenum dithiocarbamate, molybdenum naphthenate, and Molyvan 822, organo molybdenum dithiocarbamate.

  6. Thymosin beta-4 promotes mesenchymal stem cell proliferation via an interleukin-8-dependent mechanism

    SciTech Connect (OSTI)

    Jeon, Byung-Joon; Yang, Yoolhee; Kyung Shim, Su; Yang, Heung-Mo; Cho, Daeho; Ik Bang, Sa

    2013-10-15

    Mesenchymal stem cells (MSCs) hold great promise for the field of tissue regeneration. Because only a limited number of MSCs can be obtained from each donor site, it is important to establish standard methods for MSC expansion using growth and trophic factors. Thymosin ?4 (T?4) is a novel trophic factor that has antimicrobial effects and the potential to promote tissue repair. T?4 is a ubiquitous, naturally-occurring peptide in the wound bed. Therefore, the relationship between T?4 and MSCs, especially adjacent adipose tissue-derived stem cells (ASCs), merits consideration. Exogenous T?4 treatment enhanced the proliferation of human ASCs, resulting in prominent nuclear localization of PCNA immunoreactivity. In addition, exogenous T?4 also increased IL-8 secretion and blocking of IL-8 with neutralizing antibodies decreased T?4-induced ASC proliferation, suggesting that IL-8 is a critical mediator of T?4-enhanced proliferation. Moreover, T?4 activated phosphorylation of ERK1/2 and increased the nuclear translocation of NF-?B. These observation provide that T?4 promotes the expansion of human ASCs via an IL-8-dependent mechanism that involves the ERK and NF-?B pathways. Therefore, T?4 could be used as a tool for MSC expansion in cell therapeutics. - Highlights: This is fundamental information required to correlate T?4 with MSC expansion. MSC expansion by T?4 is involved in enhancement of IL-8 and ERK/NF-?B pathway. T?4 could be used as a tool for MSC expansion in cell therapeutics.

  7. Association of scattering matrices in quantum networks

    SciTech Connect (OSTI)

    Almeida, F.A.G.; Macdo, A.M.S.

    2013-06-15

    Algorithms based on operations that associate scattering matrices in series or in parallel (analogous to impedance association in a classical circuit) are developed here. We exemplify their application by calculating the total scattering matrix of several types of quantum networks, such as star graphs and a chain of chaotic quantum dots, obtaining results with good agreement with the literature. Through a computational-time analysis we compare the efficiency of two algorithms for the simulation of a chain of chaotic quantum dots based on series association operations of (i) two-by-two centers and (ii) three-by-three ones. Empirical results point out that the algorithm (ii) is more efficient than (i) for small number of open scattering channels. A direct counting of floating point operations justifies quantitatively the superiority of the algorithm (i) for large number of open scattering channels.

  8. SEDA Sustainable Energy Development Associates LLC | Open Energy...

    Open Energy Info (EERE)

    Promotional start-up energy company that encourages business ventures into biomass as the alternative to dependency on fossil fuels. Coordinates: 38.94114, -119.750144 Show Map...

  9. Los Alamos' Terwilliger named American Crystallographic Association

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Terwilliger named American Crystallographic Association president April 27, 2016 LOS ALAMOS, N.M., April 27, 2016-Tom Terwilliger, the developer of software enabling clear X-ray imaging of protein molecules, is the 2016 President of the American Crystallographic Association. The ACA is the home to more than 1,000 scientists around the country who use X-rays, neutrons and electrons to see the shapes of molecules."This is an exciting time for crystallography because there are new ways to look

  10. Chemical inducible promoter used to obtain transgenic plants with a silent marker and organisms and cells and methods of using same for screening for mutations

    DOE Patents [OSTI]

    Zuo, Jianru; Chua, Nam-Hai

    2007-06-12

    Disclosed is a chemically inducible promoter for transforming plants or plant cells with genes which are regulatable by adding the plants or cells to a medium containing an inducer or by removing them from such medium. The promoter is inducible by a glucocorticoid, estrogen or inducer not endogenous to plants. Such promoters may be used with any plant genes that can promote shoot regeneration and development to induce shoot formation in the presence of a glucocorticoid, estrogen or inducer. The promoter may be used with antibiotic or herbicide resistance genes or other genes which are regulatable by the presence or absence of a given inducer. Also presented are organisms or cells comprising a gene wherein the natural promoter of the gene is disrupted and the gene is placed under the control of a transgenic inducible promoter. These organisms and cells and their progeny are useful for screening for conditional gain of function and loss of function mutations.

  11. Mechanism of Methane Chemical Looping Combustion with Hematite Promoted with CeO2

    SciTech Connect (OSTI)

    Miller, Duane D.; Siriwardane, Ranjani

    2013-08-01

    Chemical looping combustion (CLC) is a promising technology for fossil fuel combustion that produces sequestration-ready CO{sub 2} stream, reducing the energy penalty of CO{sub 2} separation from flue gases. An effective oxygen carrier for CLC will readily react with the fuel gas and will be reoxidized upon contact with oxygen. This study investigated the development of a CeO{sub 2}-promoted Fe{sub 2}O{sub 3}?hematite oxygen carrier suitable for the methane CLC process. Composition of CeO{sub 2} is between 5 and 25 wt % and is lower than what is generally used for supports in Fe{sub 2}O{sub 3} carrier preparations. The incorporation of CeO{sub 2} to the natural ore hematite strongly modifies the reduction behavior in comparison to that of CeO{sub 2} and hematite alone. Temperature-programmed reaction studies revealed that the addition of even 5 wt % CeO{sub 2} enhances the reaction capacity of the Fe{sub 2}O{sub 3} oxygen carrier by promoting the decomposition and partial oxidation of methane. Fixed-bed reactor data showed that the 5 wt % cerium oxides with 95 wt % iron oxide produce 2 times as much carbon dioxide in comparison to the sum of carbon dioxide produced when the oxides were tested separately. This effect is likely due to the reaction of CeO{sub 2} with methane forming intermediates, which are reactive for extracting oxygen from Fe{sub 2}O{sub 3} at a considerably faster rate than the rate of the direct reaction of Fe{sub 2}O{sub 3} with methane. These studies reveal that 5 wt % CeO{sub 2}/Fe{sub 2}O{sub 3} gives stable conversions over 15 reduction/oxidation cycles. Lab-scale reactor studies (pulsed mode) suggest the methane reacts initially with CeO{sub 2} lattice oxygen to form partial oxidation products (CO + H{sub 2}), which continue to react with oxygen from neighboring Fe{sub 2}O{sub 3}, leading to its complete oxidation to form CO{sub 2}. The reduced cerium oxide promotes the methane decomposition reaction to form C + H{sub 2}, which continue to

  12. Efficient photocatalytic hydrogen generation by silica supported and platinum promoted titanium dioxide

    SciTech Connect (OSTI)

    Joshi, Meenal M.; Labhsetwar, Nitin K.; Parwate, D.V.; Rayalu, Sadhana S.

    2013-09-01

    Graphical abstract: Titanium dioxide was supported on mesoporous silica and promoted with Pt and Ru. The supported photocatalysts show high surface area and better photocatalytic activity in visible light as compared to the benchmark Degussa P25. These photocatalysts were characterized using XRD, BET-SA, and UV-DRS techniques. The surface area of supported photocatalyst was 140.6 m{sup 2}/g which is higher than Degussa P-25. Supported photocatalyst was evaluated for hydrogen evolution via water splitting reaction using ethanol as a sacrificial donor. Hydrogen yield observed is 4791.43 ?mol/h/g of TiO{sub 2} and that for P-25 is 161 ?mol/h/g of TiO{sub 2} under visible light irradiation. The value is 30 times higher than benchmark material Degussa P-25. This photocatalyst is also found stable up to 24 h without replenishing with sacrificial donor ethanol. - Highlights: Semiconductor titanium dioxide has been supported on silica gel and promoted with Pt by simple wet impregnation route. This synthesized photocatalyst is showing high surface area of 140.6 m{sup 2}/g with crystallite size in the range of 15.44 ?. This photocatalyst is showing enhanced hydrogen yield of about 4791.43 ?mol/h/g of TiO{sub 2}. This photocatalyst is also found stable up to 24 h without replenishing with sacrificial donor ethanol. The effect of various operating parameters on supported photocatalyst also has been studied. - Abstract: Titanium dioxide was supported on mesoporous silica and promoted with Pt and Ru. The supported photocatalysts show high surface area and better photocatalytic activity in visible light as compared to the benchmark Degussa P25. These photocatalysts were characterized using XRD, BET-SA, and UV-DRS techniques. The surface area of supported photocatalyst was 140.6 m{sup 2}/g which is higher than Degussa P-25. Supported photocatalyst was evaluated for hydrogen evolution via water splitting reaction using ethanol as a sacrificial donor. Hydrogen yield

  13. Alaska Native Village CEO Association 2015 Conference

    Broader source: Energy.gov [DOE]

    The Alaska Native Village Corporation Association is hosting its 7th Annual 2015 Conference in Anchorage, Alaska. The two-day conference includes a State of Alaska update, board election best practices, Alaska's economic future, Alaska Native subsistence co-management, and more.

  14. QER- Comment of Canadian Electricity Association

    Broader source: Energy.gov [DOE]

    Dear Ms. Pickett and/or DOE staff: Attached please find comments of the Canadian Electricity Association (CEA) on the Quadrennial Energy Review. Please confirm receipt at your convenience and please advise if you require anything further. Many thanks for this opportunity to provide comments. Best regards,

  15. Aleutian Pribilof Islands Association- 2010 Project

    Broader source: Energy.gov [DOE]

    The Aleutian Pribilof Islands Association, Inc. (APIA) will conduct on-site weatherization and energy conservation education and a home energy and safety review in the communities of Akutan, Atka, False Pass, King Cove, Nelson Lagoon, Nikolski, Sand Point, St. George, St. Paul, and Unalaska.

  16. QER- Comment of Electric Drive Transportation Association

    Broader source: Energy.gov [DOE]

    Please find attached the comments of the Electric Drive Transportation Association regarding the first volume of the Department of Energy’s QER. If you have questions about our submittal or require further information, please contact me using the information provided below. Thank you for the opportunity to comment. Genevieve Cullen

  17. National Science Teachers Association Area Conference

    Broader source: Energy.gov [DOE]

    The National Science Teachers Association Area Conference will be hosted in Reno, Nevada, October 22–24, 2015. The conference’s theme is “Science and Literacy: Creating Connections!” The conference will provide science educators an opportunity to develop their professional skills and network. Bioenergy Technologies Office Communications Lead Sheila Dillard will be in attendance.

  18. National Science Teachers Association Area Conference

    Broader source: Energy.gov [DOE]

    The National Science Teachers Association Area Conference will be hosted in Philadelphia, Pennsylvania, November 12–14, 2015. The conference is theme is “Revolutionary Science” and will provide science educators an opportunity to develop their professional skills and network. Bioenergy Technologies Office Communications Lead Sheila Dillard will be in attendance

  19. Fischer-Tropsch activity for non-promoted cobalt-on-alumina catalysts

    DOE Patents [OSTI]

    Singleton, Alan H.; Oukaci, Rachid; Goodwin, James G.

    2001-01-01

    Cobalt catalysts, and processes employing these inventive catalysts, for hydrocarbon synthesis. The inventive catalyst comprises cobalt on an alumina support and is not promoted with any noble or near noble metals. In one aspect of the invention, the alumina support preferably includes a dopant in an amount effective for increasing the activity of the inventive catalyst. The dopant is preferably a titanium dopant. In another aspect of the invention, the cobalt catalyst is preferably reduced in the presence of hydrogen at a water vapor partial pressure effective to increase the activity of the cobalt catalyst for hydrocarbon synthesis. The water vapor partial pressure is preferably in the range of from 0 to about 0.1 atmospheres.

  20. Sterol-dependent nuclear import of ORP1S promotes LXR regulated trans-activation of apoE

    SciTech Connect (OSTI)

    Lee, Sungsoo; Wang, Ping-Yuan; Jeong, Yangsik; Mangelsdorf, David J.; Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390-9041 ; Anderson, Richard G.W.; Michaely, Peter

    2012-10-01

    Oxysterol binding protein related protein 1S (ORP1S) is a member of a family of sterol transport proteins. Here we present evidence that ORP1S translocates from the cytoplasm to the nucleus in response to sterol binding. The sterols that best promote nuclear import of ORP1S also activate the liver X receptor (LXR) transcription factors and we show that ORP1S binds to LXRs, promotes binding of LXRs to LXR response elements (LXREs) and specifically enhances LXR-dependent transcription via the ME.1 and ME.2 enhancer elements of the apoE gene. We propose that ORP1S is a cytoplasmic sterol sensor, which transports sterols to the nucleus and promotes LXR-dependent gene transcription through select enhancer elements. -- Highlights: Black-Right-Pointing-Pointer ORP1S translocates to the nucleus in response to sterol binding. Black-Right-Pointing-Pointer The sterols that best promote nuclear import of ORP1S are LXR agonists. Black-Right-Pointing-Pointer ORP1S binds to LXRs, enhances binding of LXRs to LXREs and promotes LXR-dependent transcription of apoE.

  1. miR-4295 promotes cell proliferation and invasion in anaplastic thyroid carcinoma via CDKN1A

    SciTech Connect (OSTI)

    Shao, Mingchen; Geng, Yiwei; Lu, Peng; Xi, Ying; Wei, Sidong; Wang, Liuxing; Fan, Qingxia; Ma, Wang

    2015-09-04

    MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. However, the role of microRNAs in anaplastic thyroid carcinoma (ATC), has remained elusive. Here, we identified that miR-4295 promotes ATC cell proliferation by negatively regulates its target gene CDKN1A. In ATC cell lines, CCK-8 proliferation assay indicated that the cell proliferation was promoted by miR-4295, while miR-4295 inhibitor significantly inhibited the cell proliferation. Transwell assay showed that miR-4295 mimics significantly promoted the migration and invasion of ATC cells, whereas miR-4295 inhibitors significantly reduced cell migration and invasion. luciferase assays confirmed that miR-4295 directly bound to the 3'untranslated region of CDKN1A, and western blotting showed that miR-4295 suppressed the expression of CDKN1A at the protein levels. This study indicated that miR-4295 negatively regulates CDKN1A and promotes proliferation and invasion of ATC cell lines. Thus, miR-4295 may represent a potential therapeutic target for ATC intervention. - Highlights: • miR-4295 mimics promote the proliferation and invasion of ATC cells. • miR-4295 inhibitors inhibit the proliferation and invasion of ATC cells. • miR-4295 targets 3′UTR of CDKN1A in ATC cells. • miR-4295 negatively regulates CDKN1A in ATC cells.

  2. Catalytic conversion of syngas to mixed alcohols over Zn-Mn promoted Cu-Fe based catalyst

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lu, Yongwu; Yu, Fei; Hu, Jin; Liu, Jian

    2012-04-12

    Zn-Mn promoted Cu-Fe based catalyst was synthesized by the co-precipitation method. Mixed alcohols synthesis from syngas was studied in a half-inch tubular reactor system after the catalyst was reduced. Zn-Mn promoted Cu-Fe based catalyst was characterized by SEM-EDS, TEM, XRD, and XPS. The liquid phase products (alcohol phase and hydrocarbon phase) were analyzed by GC-MS and the gas phase products were analyzed by GC. The results showed that Zn-Mn promoted Cu-Fe based catalyst had high catalytic activity and high alcohol selectivity. The maximal CO conversion rate was 72%, and the yield of alcohol and hydrocarbons were also very high. Cumore » (111) was the active site for mixed alcohols synthesis, Fe2C (101) was the active site for olefin and paraffin synthesis. The reaction mechanism of mixed alcohols synthesis from syngas over Zn-Mn promoted Cu-Fe based catalyst was proposed. Here, Zn-Mn promoted Cu-Fe based catalyst can be regarded as a potential candidate for catalytic conversion of biomass-derived syngas to mixed alcohols.« less

  3. Global epigenomic analysis indicates protocadherin-7 activates osteoclastogenesis by promoting cellcell fusion

    SciTech Connect (OSTI)

    Nakamura, Haruhiko; Nakashima, Tomoki; Hayashi, Mikihito; Izawa, Naohiro; Yasui, Tetsuro; Aburatani, Hiroyuki; Tanaka, Sakae; Takayanagi, Hiroshi

    2014-12-12

    Highlights: Identification of epigenetically regulated genes during osteoclastogenesis. Pcdh7 is regulated by H3K4me3 and H3K27me3 during osteoclastogenesis. Pcdh7 expression is increased by RANKL during osteoclastogenesis. Establishment of novel cell fusion analysis for osteoclasts by imaging cytometer. Pcdh7 regulates osteoclastogenesis by promoting cell fusion related gene expressions. - Abstract: Gene expression is dependent not only on genomic sequences, but also epigenetic control, in which the regulation of chromatin by histone modification plays a crucial role. Histone H3 lysine 4 trimethylation (H3K4me3) and histone H3 lysine 27 trimethylation (H3K27me3) are related to transcriptionally activated and silenced sequences, respectively. Osteoclasts, the multinucleated cells that resorb bone, are generated by the fusion of precursor cells of monocyte/macrophage lineage. To elucidate the molecular and epigenetic regulation of osteoclast differentiation, we performed a chromatin immunoprecipitation sequencing (ChIP-seq) analysis for H3K4me3 and H3K27me3 in combination with RNA sequencing. We focused on the histone modification change from H3K4me3(+)H3K27me3(+) to H3K4me3(+)H3K27me3() and identified the protocadherin-7 gene (Pcdh7) to be among the genes epigenetically regulated during osteoclastogenesis. Pcdh7 was induced by RANKL stimulation in an NFAT-dependent manner. The knockdown of Pcdh7 inhibited RANKL-induced osteoclast differentiation due to the impairment of cellcell fusion, accompanied by a decreased expression of the fusion-related genes Dcstamp, Ocstamp and Atp6v0d2. This study demonstrates that Pcdh7 plays a key role in osteoclastogenesis by promoting cellcell fusion.

  4. Policy Strategies and Paths to promote Sustainable Energy Systems- The dynamic Invert Simulation Tool

    SciTech Connect (OSTI)

    Stadler, Michael; Kranzl, Lukas; Huber, Claus; Haas, Reinhard; Tsioliaridou, Elena

    2006-05-01

    The European Union has established a number of targetsregarding energy efficiency, Renewable Energy Sources (RES) and CO2reductions as the 'GREEN PAPER on Energy Efficiency', the Directive for'promotion of the use of bio-fuels or other renewable fuels fortransport' or 'Directive of the European Parliament of the Council on thepromotion of cogeneration based on a useful heat demand in the internalenergy market'. A lot of the according RES and RUE measures are notattractive for investors from an economic point of view. Thereforegovernmentsall over the world have to spend public money to promotethese technologies/measures to bring them into market. These expenditureshave to be adjusted to budget concerns and should be spent mostefficiently. Therefore, the spent money has to be dedicated totechnologies and efficiency measures with the best yield in CO2 reductionwithout wasting money. The core question: "How can public money - forpromoting sustainable energy systems - be spent most efficiently toreduce GHG-emissions?" has been well investigated by the European projectInvert. In course of this project a simulation tool has been designed toanswer this core question. This paper describes the modelling with theInvert simulation tool and shows the key features necessary forsimulating the energy system. A definition of 'Promotion SchemeEfficiency' is given which allows estimating the most cost effectivetechnologies and/or efficiency measures to reduce CO2 emissions.Investigations performed with the Invert simulation tool deliver anoptimum portfolio mix of technologies and efficiency measures for eachselected region. Within Invert seven European regions were simulated andfor the Austrian case study the detailed portfolio mix is shown andpolitical conclusions are derived.

  5. Preventing cleavage of Mer promotes efferocytosis and suppresses acute lung injury in bleomycin treated mice

    SciTech Connect (OSTI)

    Lee, Ye-Ji; Lee, Seung-Hae; Youn, Young-So; Choi, Ji-Yeon; Song, Keung-Sub; Cho, Min-Sun; Kang, Jihee Lee

    2012-08-15

    Mer receptor tyrosine kinase (Mer) regulates macrophage activation and promotes apoptotic cell clearance. Mer activation is regulated through proteolytic cleavage of the extracellular domain. To determine if membrane-bound Mer is cleaved during bleomycin-induced lung injury, and, if so, how preventing the cleavage of Mer enhances apoptotic cell uptake and down-regulates pulmonary immune responses. During bleomycin-induced acute lung injury in mice, membrane-bound Mer expression decreased, but production of soluble Mer and activity as well as expression of disintegrin and metalloproteinase 17 (ADAM17) were enhanced . Treatment with the ADAM inhibitor TAPI-0 restored Mer expression and diminished soluble Mer production. Furthermore, TAPI-0 increased Mer activation in alveolar macrophages and lung tissue resulting in enhanced apoptotic cell clearance in vivo and ex vivo by alveolar macrophages. Suppression of bleomycin-induced pro-inflammatory mediators, but enhancement of hepatocyte growth factor induction were seen after TAPI-0 treatment. Additional bleomycin-induced inflammatory responses reduced by TAPI-0 treatment included inflammatory cell recruitment into the lungs, levels of total protein and lactate dehydrogenase activity in bronchoalveolar lavage fluid, as well as caspase-3 and caspase-9 activity and alveolar epithelial cell apoptosis in lung tissue. Importantly, the effects of TAPI-0 on bleomycin-induced inflammation and apoptosis were reversed by coadministration of specific Mer-neutralizing antibodies. These findings suggest that restored membrane-bound Mer expression by TAPI-0 treatment may help resolve lung inflammation and apoptosis after bleomycin treatment. -- Highlights: ►Mer expression is restored by TAPI-0 treatment in bleomycin-stimulated lung. ►Mer signaling is enhanced by TAPI-0 treatment in bleomycin-stimulated lung. ►TAPI-0 enhances efferocytosis and promotes resolution of lung injury.

  6. Inhibition of Sirt1 promotes neural progenitors toward motoneuron differentiation from human embryonic stem cells

    SciTech Connect (OSTI)

    Zhang, Yun; Wang, Jing; Clinical Stem Cell Center, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191 ; Chen, Guian; Reproductive Medical Center, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191 ; Fan, Dongsheng; Clinical Stem Cell Center, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191 ; Deng, Min; Clinical Stem Cell Center, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing 100191

    2011-01-14

    Research highlights: {yields} Nicotinamide inhibit Sirt1. {yields} MASH1 and Ngn2 activation. {yields} Increase the expression of HB9. {yields} Motoneurons formation increases significantly. -- Abstract: Several protocols direct human embryonic stem cells (hESCs) toward differentiation into functional motoneurons, but the efficiency of motoneuron generation varies based on the human ESC line used. We aimed to develop a novel protocol to increase the formation of motoneurons from human ESCs. In this study, we tested a nuclear histone deacetylase protein, Sirt1, to promote neural precursor cell (NPC) development during differentiation of human ESCs into motoneurons. A specific inhibitor of Sirt1, nicotinamide, dramatically increased motoneuron formation. We found that about 60% of the cells from the total NPCs expressed HB9 and {beta}III-tubulin, commonly used motoneuronal markers found in neurons derived from ESCs following nicotinamide treatment. Motoneurons derived from ESC expressed choline acetyltransferase (ChAT), a positive marker of mature motoneuron. Moreover, we also examined the transcript levels of Mash1, Ngn2, and HB9 mRNA in the differentiated NPCs treated with the Sirt1 activator resveratrol (50 {mu}M) or inhibitor nicotinamide (100 {mu}M). The levels of Mash1, Ngn2, and HB9 mRNA were significantly increased after nicotinamide treatment compared with control groups, which used the traditional protocol. These results suggested that increasing Mash1 and Ngn2 levels by inhibiting Sirt1 could elevate HB9 expression, which promotes motoneuron differentiation. This study provides an alternative method for the production of transplantable motoneurons, a key requirement in the development of hESC-based cell therapy in motoneuron disease.

  7. Sox2 promotes survival of satellite glial cells in vitro

    SciTech Connect (OSTI)

    Koike, Taro Wakabayashi, Taketoshi; Mori, Tetsuji; Hirahara, Yukie; Yamada, Hisao

    2015-08-14

    Sox2 is a transcriptional factor expressed in neural stem cells. It is known that Sox2 regulates cell differentiation, proliferation and survival of the neural stem cells. Our previous study showed that Sox2 is expressed in all satellite glial cells of the adult rat dorsal root ganglion. In this study, to examine the role of Sox2 in satellite glial cells, we establish a satellite glial cell-enriched culture system. Our culture method succeeded in harvesting satellite glial cells with the somata of neurons in the dorsal root ganglion. Using this culture system, Sox2 was downregulated by siRNA against Sox2. The knockdown of Sox2 downregulated ErbB2 and ErbB3 mRNA at 2 and 4 days after siRNA treatment. MAPK phosphorylation, downstream of ErbB, was also inhibited by Sox2 knockdown. Because ErbB2 and ErbB3 are receptors that support the survival of glial cells in the peripheral nervous system, apoptotic cells were also counted. TUNEL-positive cells increased at 5 days after siRNA treatment. These results suggest that Sox2 promotes satellite glial cell survival through the MAPK pathway via ErbB receptors. - Highlights: • We established satellite glial cell culture system. • Function of Sox2 in satellite glial cell was examined using siRNA. • Sox2 knockdown downregulated expression level of ErbB2 and ErbB3 mRNA. • Sox2 knockdown increased apoptotic satellite glial cell. • Sox2 promotes satellite glial cell survival through ErbB signaling.

  8. Evaluation of Promoters for Rhodium-Based Catalysts for Mixed Alcohol Synthesis

    SciTech Connect (OSTI)

    Gerber, Mark A.; White, James F.; Gray, Michel J.; Stevens, Don J.

    2008-12-08

    Pacific Northwest National Laboratory (PNNL) and National Renewable Energy Laboratory (NREL) are conducting research to investigate the feasibility of producing mixed alcohols from biomass-derived synthesis gas (syngas). PNNL is tasked with obtaining commercially-available catalysts or preparing promising mixed-alcohol catalysts and screening them in a laboratory-scale reactor system. Commercially-available catalysts and the most promising experimental catalysts are provided to NREL for testing using a slipstream from a pilot-scale biomass gasifier. A total of 28 tests were conducted to evaluate 22 different promoters as well as an unpromoted catalyst. The following general trends were observed for the test results: The highest carbon selectivity to C2+ oxygenates occurred at the lowest reaction temperatures and accompanying lowest space time yields (STYs). The lowest carbon selectivity to C2+ oxygenates occurred at the highest reaction temperatures because of high carbon conversion to hydrocarbons. The highest C2+-oxygenate STYs occurred between 300C and 325C, with the gas hourly space velocity (GHSV) adjusted when necessary to maintain carbon conversion ranges between ~ 30 and 40 percent. Higher carbon selectivity to hydrocarbons at higher temperatures resulted in lower C2+-oxygenate STYs. When catalysts were heated to between 300C and 325C the catalysts showed evidence of some deactivation with respect to C2+ oxygenate productivity, accompanied by reduced chain growth for the hydrocarbon products. The degree of deactivation and the temperature at which it occurred varied between the different catalysts tested. Of all of the catalysts evaluated, the Li-promoted catalysts had the highest carbon selectivity to C2+ oxygenates (47 percent) under the conditions at which the maximum C2+-oxygenate STYs were obtained.

  9. Chancellor Water Colloids: Characterization and Radionuclide Association

    SciTech Connect (OSTI)

    Abdel-Fattah, Amr I.

    2012-06-18

    Concluding remarks about this paper are: (1) Gravitational settling, zeta potential, and ultrafiltration data indicate the existence of a colloidal phase of both the alpha and beta emitters in the Chancellor water; (2) The low activity combined with high dispersion homogeneity of the Chancellor water indicate that both alpha and beta emitters are not intrinsic colloids; (3) Radionuclides in the Chancellor water, particularly Pu, coexist as dissolved aqueous and sorbed phases - in other words the radionuclides are partitioned between the aqueous phase and the colloidal phase; (4) The presence of Pu as a dissolved species in the aqueous phase, suggests the possibility of Pu in the (V) oxidation state - this conclusion is supported by the similarity of the k{sub d} value of Pu determined in the current study to that determined for Pu(V) sorbed onto smectite colloids, and the similar electrokinetic behavior of the Chancellor water colloids to smectite colloids; (5) About 50% of the Pu(V) is in the aqueous phase and 50% is sorbed on colloids (mass concentration of colloids in the Chancellor water is 0.12 g/L); (6) The k{sub d} of the Pu and the beta emitters (fission products) between aqueous and colloidal phases in the Chancellor water is {approx}8.0 x 10{sup 3} mL/g using two different activity measurement techniques (LSC and alpha spectroscopy); (7) The gravitational settling and size distributions of the association colloids indicate that the properties (at least the physical ones) of the colloids to which the alpha emitters are associated with seem to be different that the properties of the colloids to which the beta emitters are associated with - the beta emitters are associated with very small particles ({approx}50 - 120 nm), while the alpha emitters are associated with relatively larger particles; and (8) The Chancellor water colloids are extremely stable under the natural pH and ionic strength conditions, indicating high potential for transport in the

  10. Hydrocracking and hydroisomerization of long-chain alkanes and polyolefins over metal-promoted anion-modified transition metal oxides

    DOE Patents [OSTI]

    Venkatesh, Koppampatti R.; Hu, Jianli; Tierney, John W.; Wender, Irving

    2001-01-01

    A method of cracking a feedstock by contacting the feedstock with a metal-promoted anion-modified metal oxide catalyst in the presence of hydrogen gas. The metal oxide of the catalyst is one or more of ZrO.sub.2, HfO.sub.2, TiO.sub.2 and SnO.sub.2, and the feedstock is principally chains of at least 20 carbon atoms. The metal-promoted anion-modified metal oxide catalyst contains one or more of Pt, Ni, Pd, Rh, Ir, Ru, (Mn & Fe) or mixtures of them present between about 0.2% to about 15% by weight of the catalyst. The metal-promoted anion-modified metal oxide catalyst contains one or more of SO.sub.4, WO.sub.3, or mixtures of them present between about 0.5% to about 20% by weight of the catalyst.

  11. National 8a Association 2016 Winter Conference | Department of...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    National 8a Association 2016 Winter Conference National 8a Association 2016 Winter Conference February 9, 2016 9:00AM EST to February 10, 2016 5:00PM EST National 8(a) Association...

  12. Influence of dioxygen on the promotional effect of Bi during Pt-catalyzed oxidation of 1,6-hexanediol

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Xie, Jiahan; Huang, Benjamin; Yin, Kehua; Pham, Hien N.; Unocic, Raymond R.; Datye, Abhaya K.; Davis, Robert J.

    2016-05-24

    In this study, a series of carbon-supported, Bi-promoted Pt catalysts with various Bi/Pt atomic ratios was prepared by selectively depositing Bi on Pt nanoparticles. The catalysts were evaluated for 1,6-hexanediol oxidation activity in aqueous solvent under different dioxygen pressures. The rate of diol oxidation on the basis of Pt loading over a Bi-promoted catalyst was 3 times faster than that of an unpromoted Pt catalyst under 0.02 MPa of O2, whereas the unpromoted catalyst was more active than the promoted catalyst under 1 MPa of O2. After liquid-phase catalyst pretreatment and 1,6-hexanediol oxidation, migration of Bi on the carbon supportmore » was observed. The reaction order in O2 was 0 over Bi-promoted Pt/C in comparison to 0.75 over unpromoted Pt/C in the range of 0.02–0.2 MPa of O2. Under low O2 pressure, rate measurements in D2O instead of H2O solvent revealed a moderate kinetic isotope effect (rateH2O/rateD2O) on 1,6-hexanediol oxidation over Pt/C (KIE = 1.4), whereas a negligible effect was observed on Bi-Pt/C (KIE = 0.9), indicating that the promotional effect of Bi could be related to the formation of surface hydroxyl groups from the reaction of dioxygen and water. No significant change in product distribution or catalyst stability was observed with Bi promotion, regardless of the dioxygen pressure.« less

  13. Selection for Unequal Densities of Sigma70 Promoter-like Signalsin Different Regions of Large Bacterial Genomes

    SciTech Connect (OSTI)

    Huerta, Araceli M.; Francino, M. Pilar; Morett, Enrique; Collado-Vides, Julio

    2006-03-01

    The evolutionary processes operating in the DNA regions that participate in the regulation of gene expression are poorly understood. In Escherichia coli, we have established a sequence pattern that distinguishes regulatory from nonregulatory regions. The density of promoter-like sequences, that are recognizable by RNA polymerase and may function as potential promoters, is high within regulatory regions, in contrast to coding regions and regions located between convergently-transcribed genes. Moreover, functional promoter sites identified experimentally are often found in the subregions of highest density of promoter-like signals, even when individual sites with higher binding affinity for RNA polymerase exist elsewhere within the regulatory region. In order to investigate the generality of this pattern, we have used position weight matrices describing the -35 and -10 promoter boxes of E. coli to search for these motifs in 43 additional genomes belonging to most established bacterial phyla, after specific calibration of the matrices according to the base composition of the noncoding regions of each genome. We have found that all bacterial species analyzed contain similar promoter-like motifs, and that, in most cases, these motifs follow the same genomic distribution observed in E. coli. Differential densities between regulatory and nonregulatory regions are detectable in most bacterial genomes, with the exception of those that have experienced evolutionary extreme genome reduction. Thus, the phylogenetic distribution of this pattern mirrors that of genes and other genomic features that require weak selection to be effective in order to persist. On this basis, we suggest that the loss of differential densities in the reduced genomes of host-restricted pathogens and symbionts is the outcome of a process of genome degradation resulting from the decreased efficiency of purifying selection in highly structured small populations. This implies that the differential

  14. Oak Ridge Associated VERIFICATION Universities OF OF

    Office of Legacy Management (LM)

    y /I/ i Prepared by Oak Ridge Associated VERIFICATION Universities OF OF - Prepared for the Decontamination and 1985 AND 1986 REMEDIAL ACTIONS Decommissioning Division NIAGARA FALLS STORAGE SITE U.S. Department VICINITY PROPERTIES of Energy of Energy LEWISTON, NEW YORK 3I~~~~ ~~~J. D. BERGER l I I I I I Environmental Survey and Site Assessment Program Energy/Environment Systems Division FINAL REPORT JULY 1990 VERIFICATION OF 1985 AND 1986 REMEDIAL ACTIONS NIAGARA FALLS STORAGE SITE VICINITY

  15. Induced seismicity associated with enhanced geothermal system

    SciTech Connect (OSTI)

    Majer, Ernest; Majer, Ernest L.; Baria, Roy; Stark, Mitch; Oates, Stephen; Bommer, Julian; Smith, Bill; Asanuma, Hiroshi

    2006-09-26

    Enhanced Geothermal Systems (EGS) offer the potential to significantly add to the world energy inventory. As with any development of new technology, some aspects of the technology has been accepted by the general public, but some have not yet been accepted and await further clarification before such acceptance is possible. One of the issues associated with EGS is the role of microseismicity during the creation of the underground reservoir and the subsequent extraction of the energy. The primary objectives of this white paper are to present an up-to-date review of the state of knowledge about induced seismicity during the creation and operation of enhanced geothermal systems, and to point out the gaps in knowledge that if addressed will allow an improved understanding of the mechanisms generating the events as well as serve as a basis to develop successful protocols for monitoring and addressing community issues associated with such induced seismicity. The information was collected though literature searches as well as convening three workshops to gather information from a wide audience. Although microseismicity has been associated with the development of production and injection operations in a variety of geothermal regions, there have been no or few adverse physical effects on the operations or on surrounding communities. Still, there is public concern over the possible amount and magnitude of the seismicity associated with current and future EGS operations. It is pointed out that microseismicity has been successfully dealt with in a variety of non-geothermal as well as geothermal environments. Several case histories are also presented to illustrate a variety of technical and public acceptance issues. It is concluded that EGS Induced seismicity need not pose any threat to the development of geothermal resources if community issues are properly handled. In fact, induced seismicity provides benefits because it can be used as a monitoring tool to understand the

  16. Electron Temperature Structures Associated With Magnetic Tearing

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structures Associated With Magnetic Tearing Modes in the Madison Symmetric Torus By Hillary Dianne Stephens A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy (Physics) at the UNIVERSITY OF WISCONSIN - MADISON 2010 i Abstract Tearing mode induced magnetic islands have a significant impact on the thermal char- acteristics of magnetically confined plasmas such as those in the reversed-field pinch. Using a state-of-the-art Thomson scattering

  17. QER- Comment of Natural Gas Supply Association

    Broader source: Energy.gov [DOE]

    TO: Quadrennial Energy Review Task Force In response to the Department of Energy’s August 25, 2014 Federal Register Notice seeking input on the Quadrennial Energy Review, attached are comments from the Natural Gas Supply Association. Thank you for this opportunity to share our views on the important issue of energy infrastructure. If we can be of further assistance, please let me know. Regards,

  18. National Association of the Remodeling Industry (NARI) | Open...

    Open Energy Info (EERE)

    Association of the Remodeling Industry (NARI) Jump to: navigation, search Name: National Association of the Remodeling Industry (NARI) Place: Des Plaines, IL Website: www.nari.org...

  19. Association of Brazilian Energy Service Companies ABESCO | Open...

    Open Energy Info (EERE)

    of Brazilian Energy Service Companies (ABESCO) Place: Brazil Product: Brasil's ESCO association References: Association of Brazilian Energy Service Companies (ABESCO)1...

  20. Electric and Gas Industries Association | Open Energy Information

    Open Energy Info (EERE)

    Gas Industries Association Jump to: navigation, search Name: Electric and Gas Industries Association Place: Sacramento, CA Zip: 95821 Website: www.egia.org Coordinates:...

  1. Monitoring CO2 intrusion and associated geochemical transformations...

    Office of Scientific and Technical Information (OSTI)

    Monitoring CO2 intrusion and associated geochemical transformations in a shallow ... Citation Details In-Document Search Title: Monitoring CO2 intrusion and associated ...

  2. Soil carbon sequestration and land use change associated with...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Soil carbon sequestration and land use change associated with biofuel production: empirical evidence Title Soil carbon sequestration and land use change associated with biofuel...

  3. Brazilian Association of Biomass Industries ABIB | Open Energy...

    Open Energy Info (EERE)

    Brazilian Association of Biomass Industries ABIB Jump to: navigation, search Name: Brazilian Association of Biomass Industries (ABIB) Place: Curitiba, Parana, Brazil Sector:...

  4. US Solar Energy Industries Association SEIA | Open Energy Information

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    Energy Industries Association SEIA Jump to: navigation, search Name: US Solar Energy Industries Association (SEIA) Place: Washington, Washington, DC Zip: 20005 Sector: Solar...

  5. Boston Area Solar Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Boston Area Solar Energy Association Jump to: navigation, search Name: Boston Area Solar Energy Association Address: Box 44-1017 Place: Somerville, Massachusetts Zip: 02114 Region:...

  6. Mexico National Solar Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Solar Energy Association Jump to: navigation, search Name: Mexico National Solar Energy Association Address: Calzada Acoxpa no 524 Desp 506-B Colonia Prado Coapa (14350) Mexico...

  7. Northern California Solar Energy Association NCSEA | Open Energy...

    Open Energy Info (EERE)

    Association NCSEA Jump to: navigation, search Name: Northern California Solar Energy Association (NCSEA) Place: Berkeley, California Zip: 94703 Sector: Solar Product: Berkely...

  8. New York Solar Energy Industries Association | Open Energy Information

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    New York Solar Energy Industries Association Name: New York Solar Energy Industries Association Address: 533 Woodford Avenue Place: Endicott, New York Zip: 13760 Region: Northeast...

  9. Northern California Solar Energy Association | Open Energy Information

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    Association Jump to: navigation, search Name: Northern California Solar Energy Association Address: PO Box 3008 Place: Berkeley, California Zip: 94703 Region: Bay Area Website:...

  10. Maryland DC Virginia Solar Energy Industries Association MDV...

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    DC Virginia Solar Energy Industries Association MDV SEIA Jump to: navigation, search Name: Maryland-DC-Virginia Solar Energy Industries Association (MDV-SEIA) Place: Bethesda,...

  11. Indian Wind Power Association IWPA | Open Energy Information

    Open Energy Info (EERE)

    Power Association IWPA Jump to: navigation, search Name: Indian Wind Power Association (IWPA) Place: Chennai, Tamil Nadu, India Zip: 600 020 Sector: Wind energy Product:...

  12. Development Wells At Long Valley Caldera Geothermal Area (Associates...

    Open Energy Info (EERE)

    Associates, 1987) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Development Wells At Long Valley Caldera Geothermal Area (Associates, 1987)...

  13. Maryland-National Capital Building Industry Association Regulatory...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Maryland-National Capital Building Industry Association Regulatory Burden RFI (Federal Register August 8, 2012) Maryland-National Capital Building Industry Association Regulatory ...

  14. Energy Management Company Association EMCA | Open Energy Information

    Open Energy Info (EERE)

    Company Association EMCA Jump to: navigation, search Name: Energy Management Company Association (EMCA) Place: Beijing, Beijing Municipality, China Zip: 100053 Product: ESCOEMC...

  15. PP-60 Northern Electric Cooperative Association (NEC) | Department...

    Office of Environmental Management (EM)

    PP-60 Northern Electric Cooperative Association (NEC) Presidential Permit authorizing Northern Electric Cooperative Association (NEC) to construct, operate, and to maintain ...

  16. Tamil Nadu Small and Tiny Industries Association TANSTIA | Open...

    Open Energy Info (EERE)

    Association TANSTIA Jump to: navigation, search Name: Tamil Nadu Small and Tiny Industries Association (TANSTIA) Place: India Sector: Services Product: Services & Support...

  17. Bioenergy Technologies Office: Association of Fish and Wildlife...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  18. Department of Energy Cites Brookhaven Science Associates, LLC...

    Office of Environmental Management (EM)

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  19. Large Higgs energy region in Higgs associated top pair production...

    Office of Scientific and Technical Information (OSTI)

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  20. Cambridge Energy Research Associates CERA | Open Energy Information

    Open Energy Info (EERE)

    Associates CERA Jump to: navigation, search Name: Cambridge Energy Research Associates (CERA) Place: Cambridge, Massachusetts Zip: 2142 Sector: Services Product: Prominent provider...

  1. ORISE: Worker Health Studies - Beryllium Associated Worker Registry...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Oak Ridge Institute for Science Education Beryllium Associated Worker Registry (BAWR) The Oak Ridge Institute for Science and Education (ORISE) maintains the Beryllium Associated...

  2. US Biomass Energy Research Association BERA | Open Energy Information

    Open Energy Info (EERE)

    Biomass Energy Research Association BERA Jump to: navigation, search Name: US Biomass Energy Research Association (BERA) Place: Washington, Washington, DC Zip: DC 20003 Sector:...

  3. Arizona Solar Energy Industries Association | Open Energy Information

    Open Energy Info (EERE)

    Arizona Solar Energy Industries Association Name: Arizona Solar Energy Industries Association Place: Arizona Website: www.arizonasolarindustry.org Coordinates: 34.0489281,...

  4. Comments by the Energy Storage Association to the Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    by the Energy Storage Association to the Department of Energy Electricity Advisory Council - March 13, 2014 Comments by the Energy Storage Association to the Department of Energy ...

  5. AMO Director Delivers Keynote at Copper Development Association...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Delivers Keynote at Copper Development Association Spring Meeting AMO Director Delivers Keynote at Copper Development Association Spring Meeting June 29, 2016 - 4:40pm Addthis AMO ...

  6. Comments from The National Association of Regulatory Utility...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  7. Kapil Mohan Associates Hydro Power Pvt Ltd | Open Energy Information

    Open Energy Info (EERE)

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  8. American Wind Energy Association AWEA | Open Energy Information

    Open Energy Info (EERE)

    Energy Association AWEA Jump to: navigation, search Name: American Wind Energy Association (AWEA) Place: Washington DC, Washington, DC Zip: 20001 Sector: Wind energy Product:...

  9. African Wind Energy Association - South Africa | Open Energy...

    Open Energy Info (EERE)

    Wind Energy Association - South Africa Jump to: navigation, search Name: African Wind Energy Association - South Africa Place: South Africa Website: www.afriwea.orgensouthafric...

  10. American Wind Energy Association (AWEA) | Open Energy Information

    Open Energy Info (EERE)

    AWEA) Jump to: navigation, search Logo: American Wind Energy Association (AWEA) Name: American Wind Energy Association (AWEA) Address: 1501 M Street, NW, Suite 1000 Place:...

  11. Chinese Wind Energy Equipment Association CWEEA | Open Energy...

    Open Energy Info (EERE)

    Wind Energy Equipment Association CWEEA Jump to: navigation, search Name: Chinese Wind Energy Equipment Association (CWEEA) Place: Beijing, Beijing Municipality, China Zip: 100825...

  12. Varian Semiconductor Equipment Associates Inc VSEA | Open Energy...

    Open Energy Info (EERE)

    Varian Semiconductor Equipment Associates Inc VSEA Jump to: navigation, search Name: Varian Semiconductor Equipment Associates Inc (VSEA) Place: Gloucester, Massachusetts Zip: 1930...

  13. Alternative Energy Engineering Associates LLP | Open Energy Informatio...

    Open Energy Info (EERE)

    Engineering Associates LLP Jump to: navigation, search Name: Alternative Energy Engineering Associates LLP Address: 3062 Rocky Pt Place: Bremerton Zip: 98312 Region: United States...

  14. Community and Economic Development Association of Cook County...

    Open Energy Info (EERE)

    and Economic Development Association of Cook County (CEDA) Jump to: navigation, search Name: Community and Economic Development Association of Cook County (CEDA) Place: Chicago, IL...

  15. CDWR Well Permitting Forms and Associated Documents | Open Energy...

    Open Energy Info (EERE)

    Associated Documents Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: CDWR Well Permitting Forms and Associated Documents Abstract This webpage...

  16. Marc M Siah Associates Inc | Open Energy Information

    Open Energy Info (EERE)

    Marc M Siah Associates Inc Jump to: navigation, search Name: Marc M Siah Associates Inc Region: United States Sector: Marine and Hydrokinetic Website: http: This company is listed...

  17. Renewable Energy Association UK REA | Open Energy Information

    Open Energy Info (EERE)

    Association UK REA Jump to: navigation, search Name: Renewable Energy Association UK (REA) Place: London, United Kingdom Zip: SW1Y 4AR Sector: Renewable Energy Product: Trade...

  18. NA 80 - Associate Administrator for Counterterrorism andCounterprolif...

    National Nuclear Security Administration (NNSA)

    Us Our Operations Management and Budget Office of Civil Rights Workforce Statistics NA 80 - Associate Administrator for Counterterrorism ... NA 80 - Associate...

  19. NA 70 - Associate Administrator for Defense Nuclear Security...

    National Nuclear Security Administration (NNSA)

    Us Our Operations Management and Budget Office of Civil Rights Workforce Statistics NA 70 - Associate Administrator for Defense ... NA 70 - Associate Administrator...

  20. NA 50 - Associate Administrator for Safety, Infrastructure and...

    National Nuclear Security Administration (NNSA)

    Us Our Operations Management and Budget Office of Civil Rights Workforce Statistics NA 50 - Associate Administrator for Safety, ... NA 50 - Associate Administrator...

  1. NA EA - Associate Administrator for External Affairs | National...

    National Nuclear Security Administration (NNSA)

    Us Our Operations Management and Budget Office of Civil Rights Workforce Statistics NA EA - Associate Administrator for External Affairs NA EA - Associate...

  2. Bioenergy Technologies Office: Association of Fish and Wildlife...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Office: Association of Fish and Wildlife Agencies Agricultural Conservation Committee Meeting Bioenergy Technologies Office: Association of Fish and Wildlife Agencies Agricultural ...

  3. Protocol for Addressing Induced Seismicity Associated with Enhanced...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Protocol for Addressing Induced Seismicity Associated with Enhanced Geothermal Systems (EGS) Protocol for Addressing Induced Seismicity Associated with Enhanced Geothermal Systems...

  4. New York Quantity of Production Associated with Reported Wellhead...

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

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  5. New Mexico Quantity of Production Associated with Reported Wellhead...

    Gasoline and Diesel Fuel Update (EIA)

    Quantity of Production Associated with Reported Wellhead Value (Million Cubic Feet) New ... Quantity of Natural Gas Production Associated with Reported Wellhead Value New Mexico ...

  6. New Mexico Associated-Dissolved Natural Gas, Wet After Lease...

    Gasoline and Diesel Fuel Update (EIA)

    New Mexico Associated-Dissolved Natural Gas, Wet After Lease Separation, Proved Reserves ... Wet After Lease Separation, as of Dec. 31 New Mexico Associated-Dissolved Natural Gas ...

  7. South Dakota Quantity of Production Associated with Reported...

    Gasoline and Diesel Fuel Update (EIA)

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  8. U.S. Quantity of Production Associated with Reported Wellhead...

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Quantity of Production Associated with Reported Wellhead Value (Million Cubic Feet) U.S. Quantity of Production Associated with Reported Wellhead Value (Million Cubic Feet) Decade...

  9. Spanish Hydrogen Association AeH | Open Energy Information

    Open Energy Info (EERE)

    Hydrogen Association AeH Jump to: navigation, search Name: Spanish Hydrogen Association (AeH) Place: Madrid, Spain Zip: 28760 Sector: Hydro, Hydrogen Product: Spanish conference...

  10. Marine Hydrogen and Fuel Cell Association MHFCA | Open Energy...

    Open Energy Info (EERE)

    Hydrogen and Fuel Cell Association MHFCA Jump to: navigation, search Name: Marine Hydrogen and Fuel Cell Association (MHFCA) Place: Leipzig, Germany Zip: D-04318 Sector: Hydro,...

  11. Indian Wind Turbine Manufacturers Association | Open Energy Informatio...

    Open Energy Info (EERE)

    Turbine Manufacturers Association Jump to: navigation, search Name: Indian Wind Turbine Manufacturers Association Place: Chennai, India Zip: 600 041 Sector: Wind energy Product:...

  12. Danish Wind Turbine Owners Association | Open Energy Information

    Open Energy Info (EERE)

    Turbine Owners Association Jump to: navigation, search Name: Danish Wind Turbine Owners' Association Place: Aarhus C, Denmark Zip: DK-8000 Sector: Wind energy Product: Danish Wind...

  13. M J Bradley Associates Inc | Open Energy Information

    Open Energy Info (EERE)

    J Bradley Associates Inc Jump to: navigation, search Name: M. J. Bradley & Associates, Inc. Place: Concord, Massachusetts Zip: 1742 Product: US-based strategic environment...

  14. Western New York Sustainable Energy Association | Open Energy...

    Open Energy Info (EERE)

    Sustainable Energy Association Jump to: navigation, search Name: Western New York Sustainable Energy Association Address: 27 St. Catherine's Court Place: Buffalo, New York Zip:...

  15. North Carolina Sustainable Energy Association | Open Energy Informatio...

    Open Energy Info (EERE)

    Sustainable Energy Association Jump to: navigation, search Name: North Carolina Sustainable Energy Association Place: Raleigh, North Carolina Zip: 27628 Product: A non-profit...

  16. West Virginia Quantity of Production Associated with Reported...

    U.S. Energy Information Administration (EIA) Indexed Site

    West Virginia Quantity of Production Associated with Reported Wellhead Value (Million ... Quantity of Natural Gas Production Associated with Reported Wellhead Value West Virginia ...

  17. ,"West Virginia Associated-Dissolved Natural Gas, Wet After Lease...

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","West Virginia Associated-Dissolved Natural Gas, Wet ... PM" "Back to Contents","Data 1: West Virginia Associated-Dissolved Natural Gas, Wet ...

  18. Engaging Efficiency First Chapters and Other Trade Associations...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Efficiency First Chapters and Other Trade Associations in Energy Efficiency Programs Engaging Efficiency First Chapters and Other Trade Associations in Energy Efficiency Programs ...

  19. Virginia Quantity of Production Associated with Reported Wellhead...

    U.S. Energy Information Administration (EIA) Indexed Site

    Quantity of Production Associated with Reported Wellhead Value (Million Cubic Feet) Virginia Quantity of Production Associated with Reported Wellhead Value (Million Cubic Feet) ...

  20. Utah Quantity of Production Associated with Reported Wellhead...

    U.S. Energy Information Administration (EIA) Indexed Site

    Quantity of Production Associated with Reported Wellhead Value (Million Cubic Feet) Utah Quantity of Production Associated with Reported Wellhead Value (Million Cubic Feet) Decade ...