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Sample records for alfalfa medicago sativa

  1. Integrative Analysis of Transgenic Alfalfa (Medicago sativa L...

    Office of Scientific and Technical Information (OSTI)

    Integrative Analysis of Transgenic Alfalfa (Medicago sativa L.) Suggests New Metabolic ... (Medicago sativa L.) Suggests New Metabolic Control Mechanisms for Monolignol ...

  2. Integrative Analysis of Transgenic Alfalfa (Medicago sativa L.) Suggests

    Office of Scientific and Technical Information (OSTI)

    New Metabolic Control Mechanisms for Monolignol Biosynthesis (Journal Article) | SciTech Connect Integrative Analysis of Transgenic Alfalfa (Medicago sativa L.) Suggests New Metabolic Control Mechanisms for Monolignol Biosynthesis Citation Details In-Document Search Title: Integrative Analysis of Transgenic Alfalfa (Medicago sativa L.) Suggests New Metabolic Control Mechanisms for Monolignol Biosynthesis Authors: Yun,Lee ; Fang,Chen ; Lina,Gallego-Giraldo ; Richard A.,Dixon ; Eberhard

  3. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... Integrative Analysis of Transgenic Alfalfa (Medicago sativa L.) Suggests New Metabolic Control Mechanisms for Monolignol Biosynthesis Yun,Lee ; Fang,Chen ; Lina,Gallego-Giraldo ; ...

  4. Alfalfa County, Oklahoma ASHRAE 169-2006 Climate Zone | Open...

    Open Energy Info (EERE)

    Alfalfa County, Oklahoma ASHRAE 169-2006 Climate Zone Jump to: navigation, search County Climate Zone Place Alfalfa County, Oklahoma ASHRAE Standard ASHRAE 169-2006 Climate Zone...

  5. Alfalfa Electric Coop, Inc (Kansas) | Open Energy Information

    Open Energy Info (EERE)

    Kansas) Jump to: navigation, search Name: Alfalfa Electric Coop, Inc Place: Kansas Phone Number: 580-596-3333 Website: alfalfaelectric.com Facebook: https:www.facebook.com...

  6. Alfalfa County, Oklahoma: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Alfalfa County, Oklahoma: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 36.7435919, -98.3964938 Show Map Loading map... "minzoom":false,"mapp...

  7. Animal feed compositions containing phytase derived from transgenic alfalfa and methods of use thereof

    DOE Patents [OSTI]

    Austin-Phillips, Sandra; Koegel, Richard G.; Straub, Richard J.; Cook, Mark

    2001-01-01

    A value-added composition of matter containing plant matter from transgenic alfalfa which expresses exogenous phytase activity is disclosed. The phytase activity is a gene product of an exogenous gene encoding for phytase which has been stably incorporated into the genome of alfalfa plants. The transgenic alfalfa expresses phytase activity in nutritionally-significant amounts, thereby enabling its use in animal feeds to eliminate the need for phosphorous supplementation of livestock, poultry, and fish feed rations.

  8. Animal feed compositions containing phytase derived from transgenic alfalfa and methods of use thereof

    DOE Patents [OSTI]

    Austin-Phillips, Sandra; Koegel, Richard G.; Straub, Richard J.; Cook, Mark

    1999-01-01

    A value-added composition of matter containing plant matter from transgenic alfalfa which expresses exogenous phytase activity is disclosed. The phytase activity is a gene product of an exogenous gene encoding for phytase which has been stably incorporated into the genome of alfalfa plants. The transgenic alfalfa expresses phytase activity in nutritionally-significant amounts, thereby enabling its use in animal feeds to eliminate the need for phosphorous supplementation of livestock, poultry, and fish feed rations.

  9. NMR Characterization of C3H and HCT Down-Regulated Alfalfa Lignin (Journal

    Office of Scientific and Technical Information (OSTI)

    Article) | SciTech Connect NMR Characterization of C3H and HCT Down-Regulated Alfalfa Lignin Citation Details In-Document Search Title: NMR Characterization of C3H and HCT Down-Regulated Alfalfa Lignin Authors: Yunqiao,Pu ; Fang,Chen ; Angela,Ziebell ; Brian H.,Davison ; Arthur J.,Ragauskas ; , Publication Date: 2009-12-01 OSTI Identifier: 1151964 Resource Type: Journal Article Resource Relation: Journal Name: BioEnergy Research; Journal Volume: 2; Journal Issue: 4 Research Org: BioEnergy

  10. Jet Fuel from Camelina: Jet Fuel From Camelina Sativa: A Systems Approach

    SciTech Connect (OSTI)

    2012-01-01

    PETRO Project: NC State will genetically modify the oil-crop plant Camelina sativa to produce high quantities of both modified oils and terpenes. These components are optimized for thermocatalytic conversion into energy-dense drop-in transportation fuels. The genetically engineered Camelina will capture more carbon than current varieties and have higher oil yields. The Camelina will be more tolerant to drought and heat, which makes it suitable for farming in warmer and drier climate zones in the US. The increased productivity of NC States-enhanced Camelina and the development of energy-effective harvesting, extraction, and conversion technology could provide an alternative non-petrochemical source of fuel.

  11. A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Dalal, Jyoti; Lopez, Harry; Vasani, Naresh B.; Hu, Zhaohui; Swift, Jennifer E.; Yalamanchili, Roopa; Dvora, Mia; Lin, Xiuli; Xie, Deyu; Qu, Rongda; et al

    2015-10-29

    Camelina sativa is an oilseed crop with great potential for biofuel production on marginal land. The seed oil from camelina has been converted to jet fuel and improved fuel efficiency in commercial and military test flights. Hydrogenation-derived renewable diesel from camelina is environmentally superior to that from canola due to lower agricultural inputs, and the seed meal is FDA approved for animal consumption. However, relatively low yield makes its farming less profitable. Our study is aimed at increasing camelina seed yield by reducing carbon loss from photorespiration via a photorespiratory bypass. Genes encoding three enzymes of the Escherichia coli glycolatemore » catabolic pathway were introduced: glycolate dehydrogenase (GDH), glyoxylate carboxyligase (GCL) and tartronic semialdehyde reductase (TSR). These enzymes compete for the photorespiratory substrate, glycolate, convert it to glycerate within the chloroplasts, and reduce photorespiration. As a by-product of the reaction, CO2 is released in the chloroplast, which increases photosynthesis. Camelina plants were transformed with either partial bypass (GDH), or full bypass (GDH, GCL and TSR) genes. Furthermore, transgenic plants were evaluated for physiological and metabolic traits.« less

  12. The structure of Medicago truncatula δ1-pyrroline-5-carboxylate reductase provides new insights into regulation of proline biosynthesis in plants

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ruszkowski, Milosz; Nocek, Boguslaw; Forlani, Giuseppe; Dauter, Zbigniew

    2015-10-30

    The two pathways for proline biosynthesis in higher plants share the last step, the conversion of δ1-pyrroline-5-carboxylate (P5C) to L-proline, which is catalyzed by P5C reductase (P5CR, EC 1.5.1.2) with the use of NAD(P)H as a coenzyme. There is increasing amount of evidence to suggest a complex regulation of P5CR activity at the post-translational level, yet the molecular basis of these mechanisms is unknown. Here we report the three-dimensional structure of the P5CR enzyme from the model legume Medicago truncatula (Mt). The crystal structures of unliganded MtP5CR decamer, and its complexes with the products NAD+, NADP+, and L-proline were refinedmore » using x-ray diffraction data (at 1.7, 1.85, 1.95, and 2.1 Å resolution, respectively). Based on the presented structural data, the coenzyme preference for NADPH over NADH was explained, and NADPH is suggested to be the only coenzyme used by MtP5CR in vivo. Moreover, the insensitivity of MtP5CR to feed-back inhibition by proline, revealed by enzymatic analysis, was correlated with structural features. Additionally, a mechanism for the modulation of enzyme activity by chloride anions is discussed, as well as the rationale for the possible development of effective enzyme inhibitors.« less

  13. Novel, highly expressed late nodulin gene (LjNOD16) from Lotus japonicus

    SciTech Connect (OSTI)

    Kapranov, P.; Bruijn, F.J. de; Szczyglowski, K.

    1997-04-01

    We have isolated a Lotus japonicus cDNA corresponding to a highly abundant, late nodule-specific RNA species that encodes a polypeptide with a predicted molecular mass of 15.6 kD. The protein and its corresponding gene were designated NIj16 and LjNOD16, respectively. LjNOD16 was found to be expressed only in the infected cells of L. japonicus nodules. Related DNA sequences could be identified in the genomes of both Glycine max and Medicago sativa. In the latter, a homologous mRNA species was detected in the nodules. Unlike LiNOD16, its alfalfa homologs appear to represent low-abundance mRNA species. However, the proteins corresponding to the LjNOD16 and its alfalfa homolog could be detected at similar levels in nodules but not in roots of both legume species. The predicted amino acid sequence analysis of nodulin NIj16 revealed the presence of a long {alpha}-helical region and a positively charged C terminus. The former domain has a very high propensity to form a coiled-coil type structure, indicating that nodulin NIj16 may interact with an as-yet-unidentified protein target(s) in the nodule-infected cells. Homology searches revealed no significant similarities to any known sequences in the databases, with the exception of two related, anonymous Arabidopsis expressed sequence tags.

  14. The structure of Medicago truncatula ?1-pyrroline-5-carboxylate reductase provides new insights into regulation of proline biosynthesis in plants

    SciTech Connect (OSTI)

    Ruszkowski, Milosz; Nocek, Boguslaw; Forlani, Giuseppe; Dauter, Zbigniew

    2015-10-30

    The two pathways for proline biosynthesis in higher plants share the last step, the conversion of ?1-pyrroline-5-carboxylate (P5C) to L-proline, which is catalyzed by P5C reductase (P5CR, EC 1.5.1.2) with the use of NAD(P)H as a coenzyme. There is increasing amount of evidence to suggest a complex regulation of P5CR activity at the post-translational level, yet the molecular basis of these mechanisms is unknown. Here we report the three-dimensional structure of the P5CR enzyme from the model legume Medicago truncatula (Mt). The crystal structures of unliganded MtP5CR decamer, and its complexes with the products NAD+, NADP+, and L-proline were refined using x-ray diffraction data (at 1.7, 1.85, 1.95, and 2.1 resolution, respectively). Based on the presented structural data, the coenzyme preference for NADPH over NADH was explained, and NADPH is suggested to be the only coenzyme used by MtP5CR in vivo. Moreover, the insensitivity of MtP5CR to feed-back inhibition by proline, revealed by enzymatic analysis, was correlated with structural features. Additionally, a mechanism for the modulation of enzyme activity by chloride anions is discussed, as well as the rationale for the possible development of effective enzyme inhibitors.

  15. Purification, crystallization and preliminary X-ray diffraction analysis of pathogen-inducible oxygenase (PIOX) from Oryza sativa

    SciTech Connect (OSTI)

    Lloyd, Tracy; Krol, Adam; Campanaro, Danielle; Malkowski, Michael

    2006-04-01

    The heme-containing membrane-associated fatty-acid ?-dioxygenase pathogen-inducible oxygenase (PIOX) from O. sativa has been crystallized and a data set collected to 3.0 using a rotating-anode generator and R-AXIS IV detector. Pathogen-inducible oxygenase (PIOX) is a heme-containing membrane-associated protein found in monocotyledon and dicotyledon plants that utilizes molecular oxygen to convert polyunsaturated fatty acids into their corresponding 2R-hydroperoxides. PIOX is a member of a larger family of fatty-acid ?-dioxygenases that includes the mammalian cyclooxygenase enzymes cyclooxygenase 1 and 2 (COX-1 and COX-2). Single crystals of PIOX from rice (Oryza sativa) have been grown from MPD using recombinant protein expressed in Escherichia coli and subsequently extracted utilizing decyl maltoside as the solubilizing detergent. Crystals diffract to 3.0 resolution using a rotating-anode generator and R-AXIS IV detector, and belong to space group P1. Based on the Matthews coefficient and self-rotation function analyses, there are presumed to be four molecules in the asymmetric unit related by noncrystallographic 222 symmetry.

  16. Identification of MicroRNAs and transcript targets in Camelina sativa by deep sequencing and computational methods

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Poudel, Saroj; Aryal, Niranjan; Lu, Chaofu; Wang, Tai

    2015-03-31

    Camelina sativa is an annual oilseed crop that is under intensive development for renewable resources of biofuels and industrial oils. MicroRNAs, or miRNAs, are endogenously encoded small RNAs that play key roles in diverse plant biological processes. Here, we conducted deep sequencing on small RNA libraries prepared from camelina leaves, flower buds and two stages of developing seeds corresponding to initial and peak storage products accumulation. Computational analyses identified 207 known miRNAs belonging to 63 families, as well as 5 novel miRNAs. These miRNAs, especially members of the miRNA families, varied greatly in different tissues and developmental stages. The predictedmore »miRNA target genes are involved in a broad range of physiological functions including lipid metabolism. This report is the first step toward elucidating roles of miRNAs in C. sativa and will provide additional tools to improve this oilseed crop for biofuels and biomaterials.« less

  17. A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa

    SciTech Connect (OSTI)

    Dalal, Jyoti; Lopez, Harry; Vasani, Naresh B.; Hu, Zhaohui; Swift, Jennifer E.; Yalamanchili, Roopa; Dvora, Mia; Lin, Xiuli; Xie, Deyu; Qu, Rongda; Sederoff, Heike W.

    2015-10-29

    Camelina sativa is an oilseed crop with great potential for biofuel production on marginal land. The seed oil from camelina has been converted to jet fuel and improved fuel efficiency in commercial and military test flights. Hydrogenation-derived renewable diesel from camelina is environmentally superior to that from canola due to lower agricultural inputs, and the seed meal is FDA approved for animal consumption. However, relatively low yield makes its farming less profitable. Our study is aimed at increasing camelina seed yield by reducing carbon loss from photorespiration via a photorespiratory bypass. Genes encoding three enzymes of the Escherichia coli glycolate catabolic pathway were introduced: glycolate dehydrogenase (GDH), glyoxylate carboxyligase (GCL) and tartronic semialdehyde reductase (TSR). These enzymes compete for the photorespiratory substrate, glycolate, convert it to glycerate within the chloroplasts, and reduce photorespiration. As a by-product of the reaction, CO2 is released in the chloroplast, which increases photosynthesis. Camelina plants were transformed with either partial bypass (GDH), or full bypass (GDH, GCL and TSR) genes. Furthermore, transgenic plants were evaluated for physiological and metabolic traits.

  18. Bio-energy feedstock yields and their water quality benefits in Mississippi

    SciTech Connect (OSTI)

    Parajuli, Prem B.

    2011-08-10

    Cellulosic and agricultural bio-energy crops can, under careful management, be harvested as feedstock for bio-fuels production and provide environmental benefits. However, it is required to quantify their relative advantages in feedstock production and water quality. The primary objective of this research was to evaluate potential feedstock yield and water quality benefit scenarios of bioenergy crops: Miscanthus (Miscanthus-giganteus), Switchgrass (Panicum virgatum), Johnsongrass (Sorghum halepense), Alfalfa (Medicago sativa L.), Soybean {Glycine max (L.) Merr.}, and Corn (Lea mays) in the Upper Pearl River watershed (UPRW), Mississippi using a Soil and Water Assessment Tool (SWAT). The SWAT model was calibrated (January 1981 to December 1994) and validated (January 1995 to September 2008) using monthly measured stream flow data. The calibrated and validated model determined good to very good performance for stream flow prediction (R2 and E from 0.60 to 0.86). The RMSE values (from 14 m3 s-1 to 37 m3 s-1) were estimated at similar levels of errors during model calibration and validation. The long-term average annual potential feedstock yield as an alternative energy source was determined the greatest when growing Miscanthus grass (373,849 Mg) as followed by Alfalfa (206,077 Mg), Switchgrass (132,077 Mg), Johnsongrass (47,576 Mg), Soybean (37,814 Mg), and Corn (22,069 Mg) in the pastureland and cropland of the watershed. Model results determined that average annual sediment yield from the Miscanthus grass scenario determined the least (1.16 Mg/ha) and corn scenario the greatest (12.04 Mg/ha). The SWAT model simulated results suggested that growing Miscanthus grass in the UPRW would have the greatest potential feedstock yield and water quality benefits.

  19. Gold Nanoparticles by Alfalfa Plants

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the well-known Greek legend the touch of King Midas would convert anything to metallic gold. Recently, a team working at SSRL lead by Professor Jorge Gardea-Torresdey from the...

  20. The Medicago HapMap Project

    SciTech Connect (OSTI)

    Bharti, Arvind

    2010-06-03

    Arvind Bharti from the National Center for Genome Resources discusses resequencing 400 plant and bacteria genotypes on June 3, 2010 at the "Sequencing, Finishing, Analysis in the Future" meeting in Santa Fe, NM

  1. Alfalfa Electric Coop, Inc | Open Energy Information

    Open Energy Info (EERE)

    861 Data Utility Id 296 Utility Location Yes Ownership C NERC Location SPP NERC SPP Yes RTO SPP Yes Activity Distribution Yes This article is a stub. You can help OpenEI by...

  2. Grain Accumulation of Selenium Species in Rice (Oryza sativa L.)

    SciTech Connect (OSTI)

    Carey, Anne-Marie; Scheckel, Kirk G.; Lombi, Enzo; Newville, Matt; Choi, Yongseong; Norton, Gareth J.; Price, Adam H.; Meharg, Andrew A.

    2012-09-05

    Efficient Se biofortification programs require a thorough understanding of the accumulation and distribution of Se species within the rice grain. Therefore, the translocation of Se species to the filling grain and their spatial unloading were investigated. Se species were supplied via cut flag leaves of intact plants and excised panicle stems subjected to a {+-} stem-girdling treatment during grain fill. Total Se concentrations in the flag leaves and grain were quantified by inductively coupled plasma mass spectrometry. Spatial accumulation was investigated using synchrotron X-ray fluorescence microtomography. Selenomethionine (SeMet) and selenomethylcysteine (SeMeSeCys) were transported to the grain more efficiently than selenite and selenate. SeMet and SeMeSeCys were translocated exclusively via the phloem, while inorganic Se was transported via both the phloem and xylem. For SeMet- and SeMeSeCys-fed grain, Se dispersed throughout the external grain layers and into the endosperm and, for SeMeSeCys, into the embryo. Selenite was retained at the point of grain entry. These results demonstrate that the organic Se species SeMet and SeMeSeCys are rapidly loaded into the phloem and transported to the grain far more efficiently than inorganic species. Organic Se species are distributed more readily, and extensively, throughout the grain than selenite.

  3. NMR Characterization of C3H and HCT Down-Regulated Alfalfa Lignin...

    Office of Scientific and Technical Information (OSTI)

    Sponsoring Org: USDOE Office of Science (SC), Biological and Environmental Research (BER) Country of Publication: United States Language: English Word Cloud More Like This Full ...

  4. Photosynthetic pigment concentrations, gas exchange and vegetative growth for selected monocots and dicots treated with two contrasting coal fly ashes

    SciTech Connect (OSTI)

    Yunusa, I.A.M.; Burchett, M.D.; Manoharan, V.; DeSilva, D.L.; Eamus, D.; Skilbeck, C.G.

    2009-07-15

    There is uncertainty as to the rates of coal fly ash needed for optimum physiological processes and growth. In the current study we tested the hyothesis that photosynthetic pigments concentrations and CO{sub 2} assimilation (A) are more sensitive than dry weights in plants grown on media amended with coal fly ash. We applied the Terrestrial Plant Growth Test (Guideline 208) protocols of the Organization for Economic Cooperation and Development (OECD) to monocots (barley (Hordeum vulgare) and ryegrass (Secale cereale)) and dicots (canola (Brasica napus), radish (Raphanus sativus), field peas (Pisum sativum), and lucerne (Medicago sativa)) on media amended with fly ashes derived from semi-bituminous (gray ash) or lignite (red ash) coals at rates of 0, 2.5, 5.0, 10, or 20 Mg ha(-1). The red ash had higher elemental concentrations and salinity than the gray ash. Fly ash addition had no significant effect on germination by any of the six species. At moderate rates ({<=}10 Mg ha{sup -1}) both ashes increased (P < 0.05) growth rates and concentrations of chlorophylls a and b, but reduced carotenoid concentrations. Addition of either ash increased A in radish and transpiration in barley. Growth rates and final dry weights were reduced for all of the six test species when addition rates exceeded 10 Mg ha{sup -1} for gray ash and 5 Mg ha{sup -1} for red ash. We concluded that plant dry weights, rather than pigment concentrations and/or instantaneous rates of photosynthesis, are more consistent for assessing subsequent growth in plants supplied with fly ash.

  5. Minnesota Agri-Power Project. Quarterly report, January--March, 1998

    SciTech Connect (OSTI)

    Wilbur, D.

    1998-05-01

    The Minnesota Valley Alfalfa Producers propose to build an alfalfa processing plant integrated with an advanced power plant system at the Granite Falls, Minnesota industrial park to provide 75 MW of base load electric power and a competitively priced source of value added alfalfa based products. This project utilizes air blown fluidized bed gasification technology to process alfalfa stems and another biomass to produce a hot, clean, low heating value gas that will be used in a gas turbine. Exhaust heat from the gas turbine will be used to generate steam to power a steam turbine and provide steam for the processing of the alfalfa leaf into a wide range of products including alfalfa leaf meal, a protein source for livestock. This progress report describes feedstock testing, feedstock supply system, performance guarantees, sales contracts, environmental permits, education, environment, economy, and project coordination and control.

  6. CX-012195: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Alfalfa Substation Control House Replacement CX(s) Applied: B4.11 Date: 05/02/2014 Location(s): Washington Offices(s): Bonneville Power Administration

  7. Microsoft Word - BPA-2010-01660-FTransfDocBIA.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    services where such power or transmission services are delivered out of the BPA's Alfalfa Substation on, or near, the Yakama Indian Reservation. BPA performed a reasonable...

  8. Microsoft Word - BPA-2010-01660-FCorrespondence.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    services where such power or transmission services are delivered out of the BPA's Alfalfa Substation on, or near, the Yakama Indian Reservation. In your letter you agreed to...

  9. EIS-0300: Notice of Intent to Prepare an Environmental Impact...

    Broader source: Energy.gov (indexed) [DOE]

    Environmental Impact Statement (EIS) regarding a proposal by the Minnesota Valley Alfalfa Producers (MnVAP) to construct and operate a 75-103 megawatt biomass fueled gasifier...

  10. Property:OutageMapURL | Open Energy Information

    Open Energy Info (EERE)

    + Agralite Electric Coop + https:pyxis-oms.comOutageMapAgraliteOutageMap.html + Alfalfa Electric Coop, Inc + https:ebill.alfalfaelectric.comwoViewermapviewer.html?config...

  11. Southern Great Plains

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    site accurately. Other extended facilities are located over wheat, pasture, rangeland, alfalfa, and native prairie. Okmulgee State Park contains 535 acres housing 100 campsites,...

  12. EIS-0238: Withdrawal of Notice of Intent to Prepare an Environmental...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Environmental Impact Statement (EIS) regarding a proposal by the Minnesota Valley Alfalfa Producers (MnVAP) to construct and operate a 75-103 megawatt biomass fueled gasifier...

  13. SSRL HEADLINES Jul 2002

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Contents of This Issue: Science Highlight - Plants with the Midas Touch: Formation of Gold Nanoparticles by Alfalfa Plants SPEAR3 Technical Progress on Track Summer Shutdown...

  14. p

    U.S. Energy Information Administration (EIA) Indexed Site

    ... Texas Ellis Beaver Hall Gray Caddo Woods Major Kiowa Potter Blaine Harper Custer Dewey Moore Alfalfa Donley Carson Washita Briscoe Randall Roberts Woodward Swisher Greer Wheeler ...

  15. EIS-0300: Minnesota Agri-Power Project: Biomass for Rural Development, Granite Falls, Minnesota

    Broader source: Energy.gov [DOE]

    This EIS analyzes DOE and the Minnesota Environmental Quality Boards' [MEQB, a Minnesota State agency] decision to support a proposal by the Minnesota Valley Alfalfa Producers (MnVAP) to construct and operate a 75–103 megawatt biomass fueled gasifier and electric generating facility, known as the Minnesota Agri-Power Plant (MAPP), and associated transmission lines and alfalfa processing facilities.

  16. Minnesota agripower project. Quarterly report, April--June 1997

    SciTech Connect (OSTI)

    Baloun, J.

    1997-07-01

    The Minnesota Valley Alfalfa Producers (MnVAP) propose to build an alfalfa processing plant integrated with an advanced power plant system at the Granite Falls, Minnesota Industrial Park to provide 75 MW of base load electric power and a competitively priced source of value added alfalfa based products. This project will utilize air blown fluidized bed gasification technology to process alfalfa stems and another biomass to produce a hot, clean, low heating value gas that will be used in a gas turbine. Exhaust heat from the gas turbine will be used to generate steam to power a steam turbine and provide steam for the processing of the alfalfa leaf into a wide range of products including alfalfa leaf meal, a protein source for livestock. The plant will demonstrate high efficiency and environmentally compatible electric power production, as well as increased economic yield from farm operations in the region. The initial phase of the Minnesota Agripower Project (MAP) will be to perform alfalfa feedstock testing, prepare preliminary designs, and develop detailed plans with estimated costs for project implementation. The second phase of MAP will include detailed engineering, construction, and startup. Full commercial operation will start in 2001.

  17. 6 PUBLIC SCOPING MEETING 7 ...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    it's in irrigated 24 agriculture up there, you see all the grapes, apples, 25 pears, alfalfa and that other stuff going on. That's 9 (509) 735-2400 BRIDGES REPORTING & LEGAL...

  18. ARM - Field Campaign - SUbsonic Aircraft: Contrail & Cloud Effects...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    center EF-10 95.788 W 795 795 795 installation planned for June 1995 Byron, OK 360 36.881 N Alfalfa ECOR Yes Yes Power and communication center EF-11 98.285 W 62695...

  19. Economic development through biomass system integration: Volume 1

    SciTech Connect (OSTI)

    DeLong, M.M.

    1995-10-01

    This report documents a feasibility study for an integrated biomass power system, where an energy crop (alfalfa) is the feedstock for a processing plant and a power plant (integrated gasification combined cycle) in a way that benefits the facility owners. Chapters describe alfalfa basics, production risks, production economics, transportation and storage, processing, products, market analysis, business analysis, environmental impact, and policy issues. 69 figs., 63 tabs.

  20. CX-007719: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    North Carolina State University - Jet Fuel from Camelina Sativa: A Systems Approach CX(s) Applied: B3.6 Date: 11/23/2011 Location(s): North Carolina Offices(s): Advanced Research Projects Agency-Energy

  1. Plant isoflavone and isoflavanone O-methyltransferase genes

    DOE Patents [OSTI]

    Broeckling, Bettina E.; Liu, Chang-Jun; Dixon, Richard A.

    2014-08-19

    The invention provides enzymes that encode O-methyltransferases (OMTs) from Medicago truncatula that allow modification to plant (iso)flavonoid biosynthetic pathways. In certain aspects of the invention, the genes encoding these enzymes are provided. The invention therefore allows the modification of plants for isoflavonoid content. Transgenic plants comprising such enzymes are also provided, as well as methods for improving disease resistance in plants. Methods for producing food and nutraceuticals, and the resulting compositions, are also provided.

  2. EIS-0238: Withdrawal of Notice of Intent to Prepare an Environmental Impact

    Broader source: Energy.gov (indexed) [DOE]

    Statement | Department of Energy Proposed Minnesota Agri-Power Plant and Associated Facilities On October 7, 1998 (63 FR 53885), U.S. Department of Energy (DOE) and the Minnesota Environmental Quality Board [MEQB, a Minnesota State agency] announced its intent to prepare a joint Environmental Impact Statement (EIS) regarding a proposal by the Minnesota Valley Alfalfa Producers (MnVAP) to construct and operate a 75-103 megawatt biomass fueled gasifier and electric generating facility, known

  3. Impact of different plants on the gas profile of a landfill cover

    SciTech Connect (OSTI)

    Reichenauer, Thomas G.; Watzinger, Andrea; Riesing, Johann; Gerzabek, Martin H.

    2011-05-15

    Research highlights: > Plants influence gas profile and methane oxidation in landfill covers. > Plants regulate water content and increase the availability of oxygen for methane oxidation. > Plant species with deep roots like alfalfa showed more stimulation of methane oxidation than plants with shallow root systems like grasses. - Abstract: Methane is an important greenhouse gas emitted from landfill sites and old waste dumps. Biological methane oxidation in landfill covers can help to reduce methane emissions. To determine the influence of different plant covers on this oxidation in a compost layer, we conducted a lysimeter study. We compared the effect of four different plant covers (grass, alfalfa + grass, miscanthus and black poplar) and of bare soil on the concentration of methane, carbon dioxide and oxygen in lysimeters filled with compost. Plants were essential for a sustainable reduction in methane concentrations, whereas in bare soil, methane oxidation declined already after 6 weeks. Enhanced microbial activity - expected in lysimeters with plants that were exposed to landfill gas - was supported by the increased temperature of the gas in the substrate and the higher methane oxidation potential. At the end of the first experimental year and from mid-April of the second experimental year, the methane concentration was most strongly reduced in the lysimeters containing alfalfa + grass, followed by poplar, miscanthus and grass. The observed differences probably reflect the different root morphology of the investigated plants, which influences oxygen transport to deeper compost layers and regulates the water content.

  4. Deep subsurface drip irrigation using coal-bed sodic water: Part I. Water and solute movement

    SciTech Connect (OSTI)

    Bern, Carleton R.; Breit, George N.; Healy, Richard W.; Zupancic, John W.; Hammack, Richard

    2013-02-01

    Water co-produced with coal-bed methane (CBM) in the semi-arid Powder River Basin of Wyoming and Montana commonly has relatively low salinity and high sodium adsorption ratios that can degrade soil permeability where used for irrigation. Nevertheless, a desire to derive beneficial use from the water and a need to dispose of large volumes of it have motivated the design of a deep subsurface drip irrigation (SDI) system capable of utilizing that water. Drip tubing is buried 92 cm deep and irrigates at a relatively constant rate year-round, while evapotranspiration by the alfalfa and grass crops grown is seasonal. We use field data from two sites and computer simulations of unsaturated flow to understand water and solute movements in the SDI fields. Combined irrigation and precipitation exceed potential evapotranspiration by 300480 mm annually. Initially, excess water contributes to increased storage in the unsaturated zone, and then drainage causes cyclical rises in the water table beneath the fields. Native chloride and nitrate below 200 cm depth are leached by the drainage. Some CBM water moves upward from the drip tubing, drawn by drier conditions above. Chloride from CBM water accumulates there as root uptake removes the water. Year over year accumulations indicated by computer simulations illustrate that infiltration of precipitation water from the surface only partially leaches such accumulations away. Field data show that 7% and 27% of added chloride has accumulated above the drip tubing in an alfalfa and grass field, respectively, following 6 years of irrigation. Maximum chloride concentrations in the alfalfa field are around 45 cm depth but reach the surface in parts of the grass field, illustrating differences driven by crop physiology. Deep SDI offers a means of utilizing marginal quality irrigation waters and managing the accumulation of their associated solutes in the crop rooting zone.

  5. Proton conduction in biopolymer exopolysaccharide succinoglycan

    SciTech Connect (OSTI)

    Kweon, Jin Jung; Lee, Kyu Won; Kim, Hyojung; Lee, Cheol Eui; Jung, Seunho; Kwon, Chanho

    2014-07-07

    Protonic currents play a vital role in electrical signalling in living systems. It has been suggested that succinoglycan plays a specific role in alfalfa root nodule development, presumably acting as the signaling molecules. In this regard, charge transport and proton dynamics in the biopolymer exopolysaccharide succinoglycan have been studied by means of electrical measurements and nuclear magnetic resonance (NMR) spectroscopy. In particular, a dielectric dispersion in the system has revealed that the electrical conduction is protonic rather electronic. Besides, our laboratory- and rotating-frame {sup 1}H NMR measurements have elucidated the nature of the protonic conduction, activation of the protonic motion being associated with a glass transition.

  6. Fluosorbent injection by-products. Final report, January 1997 through December 1999

    SciTech Connect (OSTI)

    Nelson, Sid

    2000-02-29

    Few, if any, economical alternatives exist for small coal-fired boilers that require a flue-gas desulfurization (FGD) system which does not generate wastes. A new duct-injection technology, called "Fluesorbent," was developed to help fill this gap. Fluesorbent was intentionally designed so that the saturated S02-sorbent materials can be used as beneficial soil amendments after they were used for FGD. A. Project Objective: The objective of this project was to demonstrate in the field that saturated Fluesorbent materials can be utilized beneficially on agricultural and grass lands. B. Project Results: The results of this project suggest that, indeed, saturated Fluesorbent has excellent potential as a commercial soil amendment for crops, such as alfalfa and soybeans, and for turf. Yields of alfalfa and turf were substantially increased in field testing on acidic soils by one-time applications of Fluesorbent FGD by-products. In the first two years of field testing, alfalfa yields on field plots with the FGD by-products were approximately 40% greater than on plots treated with an equivalent amount of agricultural lime. In a third, drought-influenced year, the gains were smaller. Turf grass growth was fully twice that of untreated plots and more than 10% greater than with ag-lime. A small farm trial with a modified version of the Fluesorbent by-product increased soybean yield by 25%. A small trial with corn, however, indicated no significant improvement. Even though the Fluesorbent contained fly ash, the alfalfa and turf grown in FGD-treated plots contained significantly lower levels of heavy metals than that grown in untreated or lime-treated plots. In a project greenhouse experiment, the fly ashes from five different coal boilers from around Ohio produced equivalent yields when mixed with Fluesorbent, indicating wide potential applicability of the new technology. The Fluesorbent materials were also found to be easy to extrude into pellets for use with mixed fertilizers. The by-product FGD materials also showed good potential as a granular substrate for turning volatile liquid herbicides into a dry, spreadable form. It was also shown that a significant amount of other fertilizer compounds, such as elemental sulfur, could be successfully incorporated into the pelleted products, if desired.

  7. Tuning the Spectrum for Plant Growth

    Energy Savers [EERE]

    S E L A E R P O L Y T E C H N I C I N S T I T U T E 19 N o v e m b e r 1 7 , 2 0 1 5 CWF Lactuca sativa L. LED 1 LED 2 LED 3 Green lettuce R E N S S E L A E R P O L Y T E C H N I...

  8. Agroecology of corn production in Tlaxcala, Mexico

    SciTech Connect (OSTI)

    Altieri, M.A.; Trujillo, J.

    1987-06-01

    The primary components of Tlaxcalan corn agriculture are described, including cropping patterns employed, resource management strategies, and interactions of human and biological factors. Tlaxcalan farmers grow corn in an array of polyculture and agroforestry designs that result in a series of ecological processes important for insect pest and soil fertility management. Measurements derived from a few selected fields show that trees integrated into cropping systems modify the aerial and soil environment of associated understory corn plants, influencing their growth and yields. With decreasing distance from trees, surface concentrations of most soil nutrients increase. Certain tree species affect corn yields more than others. Arthropod abundance also varies depending on their degree of association with one or more of the vegetational components of the system. Densities of predators and the corn pest Macrodactylus sp. depend greatly on the presence and phenology of adjacent alfalfa strips. Although the data were derived from nonreplicated fields, they nevertheless point out some important trends, information that can be used to design new crop association that will achieve sustained soil fertility and low pest potentials.

  9. Agricultural Industry Advanced Vehicle Technology: Benchmark Study for Reduction in Petroleum Use

    SciTech Connect (OSTI)

    Roger Hoy

    2014-09-01

    Diesel use on farms in the United States has remained relatively constant since 1985, decreasing slightly in 2009, which may be attributed to price increases and the economic recession. During this time, the United States’ harvested area also has remained relatively constant at roughly 300 million acres. In 2010, farm diesel use was 5.4% of the total United States diesel use. Crops accounting for an estimated 65% of United States farm diesel use include corn, soybean, wheat, hay, and alfalfa, respectively, based on harvested crop area and a recent analysis of estimated fuel use by crop. Diesel use in these cropping systems primarily is from tillage, harvest, and various other operations (e.g., planting and spraying) (Figure 3). Diesel efficiency is markedly variable due to machinery types, conditions of operation (e.g., soil type and moisture), and operator variability. Farm diesel use per acre has slightly decreased in the last two decades and diesel is now estimated to be less than 5% of farm costs per acre. This report will explore current trends in increasing diesel efficiency in the farm sector. The report combines a survey of industry representatives, a review of literature, and data analysis to identify nascent technologies for increasing diesel efficiency

  10. Functional and structural diversity in GH62 α-L-arabinofuranosidases from the thermophilic fungus Scytalidium thermophilum

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Kaur, Amrit Pal; Nocek, Boguslaw P.; Xu, Xiaohui; Lowden, Michael J.; Leyva, Juan Francisco; Stogios, Peter J.; Cui, Hong; Leo, Rosa Di; Powlowski, Justin; Tsang, Adrian; et al

    2015-05-01

    The genome of the thermophilic fungus Scytalidium thermophilum (strain CBS 625.91) harbours a wide range of genes involved in carbohydrate degradation, including three genes, abf62A, abf62B and abf62C, predicted to encode glycoside hydrolase family 62 (GH62) enzymes. Transcriptome analysis showed that only abf62A and abf62C are actively expressed during growth on diverse substrates including straws from barley, alfalfa, triticale and canola. The abf62A and abf62C genes were expressed in Escherichia coli and the resulting recombinant proteins were characterized. Calcium-free crystal structures of Abf62C in apo and xylotriose bound forms were determined to 1.23 and 1.48 Å resolution respectively. Site-directed mutagenesismore » confirmed Asp55, Asp171 and Glu230 as catalytic triad residues, and revealed the critical role of non-catalytic residues Asp194, Trp229 and Tyr338 in positioning the scissile α-L-arabinofuranoside bond at the catalytic site. Further, the +2R substrate-binding site residues Tyr168 and Asn339, as well as the +2NR residue Tyr226, are involved in accommodating long-chain xylan polymers. Overall, our structural and functional analysis highlights characteristic differences between Abf62A and Abf62C, which represent divergent subgroups in the GH62 family.« less

  11. Measurement of directional thermal infrared emissivity of vegetation and soils

    SciTech Connect (OSTI)

    Norman, J.M. [Wisconsin Univ., Madison, WI (United States). Dept. of Soil Science; Balick, L.K. [EG and G Energy Measurements, Inc., Las Vegas, NV (United States)

    1995-10-01

    A new method has been developed for measuring directional thermal emissivity as a function of view angle for plant canopies and soils using two infrared thermometers each sensitive to a different wavelength band. By calibrating the two infrared thermometers to 0.1C consistency, canopy directional emissivity can be estimated with typical errors less than 0.005 in the 8--14 um wavelength band, depending on clarity of the sky and corrections for CO{sub 2} absorption by the atmosphere. A theoretical justification for the method is developed along with an error analysis. Laboratory measurements were used to develop corrections for CO{sub 2}, absorption and a field calibration method is used to obtain the necessary 0.1C consistency for relatively low cost infrared thermometers. The emissivity of alfalfa (LAI=2.5) and corn (LAI=3.2) was near 0.995 and independent of view angle. Individual corn leaves had an emissivity of 0.97. A wheat (LAI=3.0) canopy had an emissivity of 0.985 at nadir and 0.975 at 75 degree view angle. The canopy emissivity values tend to be higher than values in the literature, and are useful for converting infrared thermometer measurements to kinetic temperature and interpreting satellite thermal observations.

  12. Economic feasibility analysis of water-harvesting techniques for mined-land reclamation

    SciTech Connect (OSTI)

    Nieves, L.A.; Marti, M.H.

    1981-07-01

    A water harvesting, agricultural production system, field tested as a means of reclaiming strip-mined land is described. Though the technical feasibility of the system is becoming increasingly apparent, economic feasibility and legal issues may determine its potential application. The purpose of this study is to explore the economic feasibility of the system and to provide information for use in assessing whether further investigation of water harvesting reclamation techniques is warranted. The economic feasibility of the PNL reclamation system hinges on whether its net benefits exceed those of conventional reclamation. This preliminary feasibility study assesses the net private benefits of each system using data for the Peabody Coal Company's Kayenta mine on the Black Mesa in Arizona. To compare the alternative reclamation systems, the present value of direct net benefits (income minus production and reclamation costs) is calculated for grazing (conventional reclamation) or for cropping (PNL reclamation). Three of the PNL system slope treatments have lower estimated total costs than conventional reclamation. The difference is $3895/acre for compacted slope, $3025/acre for salt-compacted slope and $2310/acre for crop-on-slope. These differences constitute a substantial cost advantage for the system on the basis of the present value of land reclamation and maintenance costs. The system also has advantages based on the estimated value of agricultural production capacity. Even the lowest yield levels considered for alfalfa, corn, and pinto beans had higher net present values than grazing.

  13. Fuel alcohol production from agricultural lignocellulosic feedstocks

    SciTech Connect (OSTI)

    Farina, G.E.; Barrier, J.W.; Forsythe, M.L. )

    1988-01-01

    A two-stage, low-temperature, ambient pressure, acid hydrolysis process that utilizes separate unit operations to convert hemicellulose and cellulose in agricultural residues and crops to fermentable sugars is being developed and tested. Based on the results of the bench-scale tests, an acid hydrolysis experimental plant to demonstrate the concepts of low-temperature acid hydrolysis on a much larger scale was built. Plant tests using corn stover have been conducted for more that a year and conversion efficiences have equaled those achieved in the laboratory. Laboratory tests to determine the potential for low-temperature acid hydrolysis of other feedstocks - including red clover, alfalfa, kobe lespedeza, winter rape, and rye grass - are being conducted. Where applicable, process modifications to include extraction before or after hydrolysis also are being studied. This paper describes the experimental plant and process, results obtained in the plant, results of alternative feedstocks testing in the laboratory, and a plan for an integrated system that will produce other fuels, feed, and food from crops grown on marginal land.

  14. Transfer Factors for Contaminant Uptake by Fruit and Nut Trees

    SciTech Connect (OSTI)

    Napier, Bruce A.; Fellows, Robert J.; Minc, Leah D.

    2013-11-20

    Transfer of radionuclides from soils into plants is one of the key mechanisms for long-term contamination of the human food chain. Nearly all computer models that address soil-to-plant uptake of radionuclides use empirically-derived transfer factors to address this process. Essentially all available soil-to-plant transfer factors are based on measurements in annual crops. Because very few measurements are available for tree fruits, samples were taken of alfalfa and oats and the stems, leaves, and fruits and nuts of almond, apple, apricot, carob, fig, grape, nectarine, pecan, pistachio (natural and grafted), and pomegranate, along with local surface soil. The samples were dried, ground, weighed, and analyzed for trace constituents through a combination of induction-coupled plasma mass spectrometry and instrumental neutron activation analysis for a wide range of naturally-occurring elements. Analysis results are presented and converted to soil-to-plant transfer factors. These are compared to commonly used and internationally recommended values. Those determined for annual crops are very similar to commonly-used values; those determined for tree fruits show interesting differences. Most macro- and micronutrients are slightly reduced in fruits; non-essential elements are reduced further. These findings may be used in existing computer models and may allow development of tree-fruit-specific transfer models.

  15. GASIFICATION FOR DISTRIBUTED GENERATION

    SciTech Connect (OSTI)

    Ronald C. Timpe; Michael D. Mann; Darren D. Schmidt

    2000-05-01

    A recent emphasis in gasification technology development has been directed toward reduced-scale gasifier systems for distributed generation at remote sites. The domestic distributed power generation market over the next decade is expected to be 5-6 gigawatts per year. The global increase is expected at 20 gigawatts over the next decade. The economics of gasification for distributed power generation are significantly improved when fuel transport is minimized. Until recently, gasification technology has been synonymous with coal conversion. Presently, however, interest centers on providing clean-burning fuel to remote sites that are not necessarily near coal supplies but have sufficient alternative carbonaceous material to feed a small gasifier. Gasifiers up to 50 MW are of current interest, with emphasis on those of 5-MW generating capacity. Internal combustion engines offer a more robust system for utilizing the fuel gas, while fuel cells and microturbines offer higher electric conversion efficiencies. The initial focus of this multiyear effort was on internal combustion engines and microturbines as more realistic near-term options for distributed generation. In this project, we studied emerging gasification technologies that can provide gas from regionally available feedstock as fuel to power generators under 30 MW in a distributed generation setting. Larger-scale gasification, primarily coal-fed, has been used commercially for more than 50 years to produce clean synthesis gas for the refining, chemical, and power industries. Commercial-scale gasification activities are under way at 113 sites in 22 countries in North and South America, Europe, Asia, Africa, and Australia, according to the Gasification Technologies Council. Gasification studies were carried out on alfalfa, black liquor (a high-sodium waste from the pulp industry), cow manure, and willow on the laboratory scale and on alfalfa, black liquor, and willow on the bench scale. Initial parametric tests evaluated through reactivity and product composition were carried out on thermogravimetric analysis (TGA) equipment. These tests were evaluated and then followed by bench-scale studies at 1123 K using an integrated bench-scale fluidized-bed gasifier (IBG) which can be operated in the semicontinuous batch mode. Products from tests were solid (ash), liquid (tar), and gas. Tar was separated on an open chromatographic column. Analysis of the gas product was carried out using on-line Fourier transform infrared spectroscopy (FT-IR). For selected tests, gas was collected periodically and analyzed using a refinery gas analyzer GC (gas chromatograph). The solid product was not extensively analyzed. This report is a part of a search into emerging gasification technologies that can provide power under 30 MW in a distributed generation setting. Larger-scale gasification has been used commercially for more than 50 years to produce clean synthesis gas for the refining, chemical, and power industries, and it is probable that scaled-down applications for use in remote areas will become viable. The appendix to this report contains a list, description, and sources of currently available gasification technologies that could be or are being commercially applied for distributed generation. This list was gathered from current sources and provides information about the supplier, the relative size range, and the status of the technology.

  16. Biomass Biorefinery for the production of Polymers and Fuels

    SciTech Connect (OSTI)

    Dr. Oliver P. Peoples

    2008-05-05

    The conversion of biomass crops to fuel is receiving considerable attention as a means to reduce our dependence on foreign oil imports and to meet future energy needs. Besides their use for fuel, biomass crops are an attractive vehicle for producing value added products such as biopolymers. Metabolix, Inc. of Cambridge proposes to develop methods for producing biodegradable polymers polyhydroxyalkanoates (PHAs) in green tissue plants as well as utilizating residual plant biomass after polymer extraction for fuel generation to offset the energy required for polymer extraction. The primary plant target is switchgrass, and backup targets are alfalfa and tobacco. The combined polymer and fuel production from the transgenic biomass crops establishes a biorefinery that has the potential to reduce the nations dependence on foreign oil imports for both the feedstocks and energy needed for plastic production. Concerns about the widespread use of transgenic crops and the growers ability to prevent the contamination of the surrounding environment with foreign genes will be addressed by incorporating and expanding on some of the latest plant biotechnology developed by the project partners of this proposal. This proposal also addresses extraction of PHAs from biomass, modification of PHAs so that they have suitable properties for large volume polymer applications, processing of the PHAs using conversion processes now practiced at large scale (e.g., to film, fiber, and molded parts), conversion of PHA polymers to chemical building blocks, and demonstration of the usefulness of PHAs in large volume applications. The biodegradability of PHAs can also help to reduce solid waste in our landfills. If successful, this program will reduce U.S. dependence on imported oil, as well as contribute jobs and revenue to the agricultural economy and reduce the overall emissions of carbon to the atmosphere.

  17. Catalytic gasification studies in a pressurized fluid-bed unit

    SciTech Connect (OSTI)

    Mudge, L.K.; Baker, E.G.; Mitchell, D.H.; Robertus, R.J.; Brown, M.D.

    1983-07-01

    The purpose of the project is to evaluate the technical and economic feasibility of producing specific gas products via the catalytic gasification of biomass. This report presents the results of research conducted from October 1980 to November 1982. In the laboratory scale studis, active catalysts were developed for generation of synthesis gases from wood by steam gasification. A trimetallic catalyst, Ni-Co-Mo on silica-alumina doped with 2 wt % Na, was found to retain activity indefinitely for generation of a methanol synthesis gas from wood at 1380/sup 0/F (750/sup 0/C) and 1 atm (100 kPa) absolute pressure. Catalysts for generation of a methane-rich gas were deactivated rapidly and could not be regenerated as required for economic application. Sodium carbonate and potassium carbonate were effective as catalysts for conversion of wood to synthesis gases and methane-rich gas and should be economically viable. Catalytic gasification conditions were found to be suitable for processing of alternative feedstocks: bagasse, alfalfa, rice hulls, and almond hulls. The PDU was operated successfully at absolute pressures of up to 10 atm (1000 kPa) and temperatures of up to 1380/sup 0/F (750/sup 0/C). Yields of synthesis gases at elevated pressure were greater than those used for previous economic evaluations. A trimetallic catalyst, Ni-Cu-Mo on silica-alumina, did not display a long life as did the doped trimetallic catalyst used in laboratory studies. A computer program for a Radio Shack TRS-80 Model I microcomputer was developed to evaluate rapidly the economics of producing either methane or methanol from wood. The program is based on economic evaluations reported in previous studies. Improved yields from the PDU studies were found to result in a reduction of about 9 cents/gal in methanol cost.

  18. Functional and structural diversity in GH62 ?-L-arabinofuranosidases from the thermophilic fungus Scytalidium thermophilum

    SciTech Connect (OSTI)

    Kaur, Amrit Pal; Nocek, Boguslaw P.; Xu, Xiaohui; Lowden, Michael J.; Leyva, Juan Francisco; Stogios, Peter J.; Cui, Hong; Leo, Rosa Di; Powlowski, Justin; Tsang, Adrian; Savchenko, Alexei

    2015-05-01

    The genome of the thermophilic fungus Scytalidium thermophilum (strain CBS 625.91) harbours a wide range of genes involved in carbohydrate degradation, including three genes, abf62A, abf62B and abf62C, predicted to encode glycoside hydrolase family 62 (GH62) enzymes. Transcriptome analysis showed that only abf62A and abf62C are actively expressed during growth on diverse substrates including straws from barley, alfalfa, triticale and canola. The abf62A and abf62C genes were expressed in Escherichia coli and the resulting recombinant proteins were characterized. Calcium-free crystal structures of Abf62C in apo and xylotriose bound forms were determined to 1.23 and 1.48 resolution respectively. Site-directed mutagenesis confirmed Asp55, Asp171 and Glu230 as catalytic triad residues, and revealed the critical role of non-catalytic residues Asp194, Trp229 and Tyr338 in positioning the scissile ?-L-arabinofuranoside bond at the catalytic site. Further, the +2R substrate-binding site residues Tyr168 and Asn339, as well as the +2NR residue Tyr226, are involved in accommodating long-chain xylan polymers. Overall, our structural and functional analysis highlights characteristic differences between Abf62A and Abf62C, which represent divergent subgroups in the GH62 family.

  19. Soil-to-Plant Concentration Ratios for Assessing Food Chain Pathways in Biosphere Models

    SciTech Connect (OSTI)

    Napier, Bruce A.; Fellows, Robert J.; Krupka, Kenneth M.

    2007-10-01

    This report describes work performed for the U.S. Nuclear Regulatory Commissions project Assessment of Food Chain Pathway Parameters in Biosphere Models, which was established to assess and evaluate a number of key parameters used in the food-chain models used in performance assessments of radioactive waste disposal facilities. Section 2 of this report summarizes characteristics of samples of soils and groundwater from three geographical regions of the United States, the Southeast, Northwest, and Southwest, and analyses performed to characterize their physical and chemical properties. Because the uptake and behavior of radionuclides in plant roots, plant leaves, and animal products depends on the chemistry of the water and soil coming in contact with plants and animals, water and soil samples collected from these regions of the United States were used in experiments at Pacific Northwest National Laboratory to determine radionuclide soil-to-plant concentration ratios. Crops and forage used in the experiments were grown in the soils, and long-lived radionuclides introduced into the groundwater provide the contaminated water used to water the grown plants. The radionuclides evaluated include 99Tc, 238Pu, and 241Am. Plant varieties include alfalfa, corn, onion, and potato. The radionuclide uptake results from this research study show how regional variations in water quality and soil chemistry affect radionuclide uptake. Section 3 summarizes the procedures and results of the uptake experiments, and relates the soil-to-plant uptake factors derived. In Section 4, the results found in this study are compared with similar values found in the biosphere modeling literature; the studys results are generally in line with current literature, but soil- and plant-specific differences are noticeable. This food-chain pathway data may be used by the NRC staff to assess dose to persons in the reference biosphere (e.g., persons who live and work in an area potentially affected by radionuclide releases) of waste disposal facilities and decommissioning sites.

  20. Integrated Assessment of Hadley Centre (HadCM2) Climate-Change Impacts on Agricultural Productivity and Irrigation Water Supply in the Conterminous United States. Part II. Regional Agricultural Production in 2030 and 2095.

    SciTech Connect (OSTI)

    Izaurralde, R Cesar C.; Rosenberg, Norman J.; Brown, Robert A.; Thomson, Allison M.

    2003-06-30

    This study used scenarios of the HadCM2 GCM and the EPIC agroecosystem model to evaluate climate change impacts on crop yields and ecosystem processes. Baseline climate data were obtained from records for 1961-1990. The scenario runs for 2025-2034 and 2090-2099 were extracted from a HadCM2 run. EPIC was run on 204 representative farms under current climate and two 10-y periods centered on 2030 and 2095, each at CO2 concentrations of 365 and 560 ppm. Texas, New Mexico, Colorado, Utah, Arizona, and California are projected to experience significant temperature increases by 2030. Slight cooling is expected by 2030 in Alabama, Florida, Maine, Montana, Idaho, and Utah. Larger areas are projected to experience increased warming by 2095. Uniform precipitation increases are expected by 2030 in the NE. These increases are predicted to expand to the eastern half of the country by 2095. EPIC simulated yield increases for the Great Lakes, Corn Belt and Northeast regions. Simulated yields of irrigated corn yields were predicted to increase in almost all regions. Soybean yields could decrease in the Northern and Southern Plains, the Corn Belt, Delta, Appalachian, and Southeast regions and increase in the Lakes and Northeast regions. Simulated wheat yields exhibited upward yield trends under scenarios of climate change. National corn production in 2030 and 2095 could be affected by changes in three major producing regions. In 2030, corn production could increase in the Corn Belt and Lakes regions but decrease in the Northern Plains leading to an overall decrease in national production. National wheat production is expected to increase during both future periods. A proxy indicator was developed to provide a sense of where in the country, and when water would be available to satisfy change in irrigation demand for corn and alfalfa production as these are influenced by the HadCM2 scenarios and CO2-fertilization.

  1. Analysis of Factors Controlling Cell Cycle that Can Be Synchronized Nondestructively During Root Cap Development

    SciTech Connect (OSTI)

    Martha Hawes

    2011-02-04

    Publications and presentations during the final funding period, including progress in defining the substrate specificity, the primary goal of the project, are listed below. Both short-term and long-term responses mediated by PsUGT1 have been characterized in transgenic or mutant pea, alfalfa, and Arabidopsis with altered expression of PsUGT1. Additional progress includes evaluation of the relationship between control of the cell cycle by PsUGT1 and other glycosyltransferase and glycosidase enzymes that are co-regulated in the legume root cap during the onset of mitosis and differentiation. Transcriptional profiling and multidimensional protein identification technology ('MudPIT') have been used to establish the broader molecular context for the mechanism by which PsUGT1 controls cell cycle in response to environmental signals. A collaborative study with the Norwegian Forest Research Institute (who provided $10,000.00 in supplies and travel funds for collaborator Dr. Toril Eldhuset to travel to Arizona and Dr. H. H. Woo to travel to Norway) made it possible to establish that the inducible root cap system for studying carbohydrate synthesis and solubilization is expressed in gymnosperm as well as angiosperm species. This discovery provides an important tool to amplify the potential applications of the research in defining conserved cell cycle machinery across a very broad range of plant species and habitats. The final work, published during 2009, revealed an additional surprising parallel with mammalian immune responses: The cells whose production is controlled by PsUGT1 appear to function in a manner which is analogous to that of white blood cells, by trapping and killing in an extracellular manner. This may explain why mutation within the coding region of PsUGT1 and its homolog in humans (UGT1) is lethal to plants and animals. The work has been the subject of invited reviews. A postdoctoral fellow, eight undergraduate students, four M.S. students and three Ph.D. students have been supported.

  2. Geothermal source potential and utilization for methane generation and alcohol production

    SciTech Connect (OSTI)

    Austin, J.C.

    1981-11-01

    A study was conducted to assess the technical and economic feasibility of integrating a geothermally heated anaerobic digester with a fuel alcohol plant and cattle feedlot. Thin stillage produced from the alcohol production process and manure collected from the cattle feedlot would be digested in anaerobic digesters to produce biogas, a mixture of methane and carbon dioxide, and residue. The energy requirements to maintain proper digester temperatures would be provided by geothermal water. The biogas produced in the digesters would be burned in a boiler to produce low-pressure steam which would be used in the alcohol production process. The alcohol plant would be sized so that the distiller's grains byproduct resulting from the alcohol production would be adequate to supply the daily cattle feed requirements. A portion of the digester residue would substitute for alfalfa hay in the cattle feedlot ration. The major design criterion for the integrated facilty was the production of adequate distiller's grain to supply the daily requirements of 1700 head of cattle. It was determined that, for a ration of 7 pounds of distiller's grain per head per day, a 1 million gpy alcohol facility would be required. An order-of-magnitude cost estimate was prepared for the proposed project, operating costs were calculated for a facility based on a corn feedstock, the economic feasibility of the proposed project was examined by calculating its simple payback, and an analysis was performed to examine the sensitivity of the project's economic viability to variations in feedstock costs and alcohol and distiller's grain prices.

  3. HIghMass-high H I mass, H I-rich galaxies at z ? 0 sample definition, optical and H? imaging, and star formation properties

    SciTech Connect (OSTI)

    Huang, Shan; Matsushita, Satoki; Haynes, Martha P.; Giovanelli, Riccardo; Hallenbeck, Gregory; Jones, Michael G.; Adams, Elizabeth A. K.; Brinchmann, Jarle; Chengalur, Jayaram N.; Hunt, Leslie K.; Masters, Karen L.; Saintonge, Amelie; Spekkens, Kristine

    2014-09-20

    We present first results of the study of a set of exceptional H I sources identified in the 40% ALFALFA extragalactic H I survey catalog ?.40 as both being H I massive (M{sub HI}>10{sup 10} M{sub ?}) and having high gas fractions for their stellar masses: the HIghMass galaxy sample. We analyze UV- and optical-broadband and H? images to understand the nature of their relatively underluminous disks in optical and to test whether their high gas fractions can be tracked to higher dark matter halo spin parameters or late gas accretion. Estimates of their star formation rates (SFRs) based on spectral energy distribution fitting agree within uncertainties with the H? luminosity inferred current massive SFRs. The H II region luminosity functions, parameterized as dN/dlog L?L {sup ?}, have standard slopes at the luminous end (? ? 1). The global SFRs demonstrate that the HIghMass galaxies exhibit active ongoing star formation (SF) with moderate SF efficiency but, relative to normal spirals, a lower integrated SFR in the past. Because the SF activity in these systems is spread throughout their extended disks, they have overall lower SFR surface densities and lower surface brightness in the optical bands. Relative to normal disk galaxies, the majority of HIghMass galaxies have higher H? equivalent widths and are bluer in their outer disks, implying an inside-out disk growth scenario. Downbending double exponential disks are more frequent than upbending disks among the gas-rich galaxies, suggesting that SF thresholds exist in the downbending disks, probably as a result of concentrated gas distribution.

  4. Defective Pollen Wall is Required for Anther and Microspore Development in Rice and Encodes a Fatty Acyl Carrier Protein Reductase

    SciTech Connect (OSTI)

    Shi, J.; Shanklin, J.; Tan, H.; Yu, X.-H.; Liu, Y.; Liang, W.; Ranathunge, K.; Franke, R. B.; Schreiber, L.; Wang, Y.; Kai, G.; Ma, H.; Zhang, D.

    2011-06-01

    Aliphatic alcohols naturally exist in many organisms as important cellular components; however, their roles in extracellular polymer biosynthesis are poorly defined. We report here the isolation and characterization of a rice (Oryza sativa) male-sterile mutant, defective pollen wall (dpw), which displays defective anther development and degenerated pollen grains with an irregular exine. Chemical analysis revealed that dpw anthers had a dramatic reduction in cutin monomers and an altered composition of cuticular wax, as well as soluble fatty acids and alcohols. Using map-based cloning, we identified the DPW gene, which is expressed in both tapetal cells and microspores during anther development. Biochemical analysis of the recombinant DPW enzyme shows that it is a novel fatty acid reductase that produces 1-hexadecanol and exhibits >270-fold higher specificity for palmiltoyl-acyl carrier protein than for C16:0 CoA substrates. DPW was predominantly targeted to plastids mediated by its N-terminal transit peptide. Moreover, we demonstrate that the monocot DPW from rice complements the dicot Arabidopsis thaliana male sterile2 (ms2) mutant and is the probable ortholog of MS2. These data suggest that DPWs participate in a conserved step in primary fatty alcohol synthesis for anther cuticle and pollen sporopollenin biosynthesis in monocots and dicots.

  5. The structure of the catalytic domain of a plant cellulose synthase and its assembly into dimers

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Olek, Anna T.; Rayon, Catherine; Makowski, Lee; Kim, Hyung Rae; Ciesielski, Peter; Badger, John; Paul, Lake N.; Ghosh, Subhangi; Kihara, Daisuke; Crowley, Michael; et al

    2014-07-10

    Cellulose microfibrils are para-crystalline arrays of several dozen linear (1→4)-β-d-glucan chains synthesized at the surface of the cell membrane by large, multimeric complexes of synthase proteins. Recombinant catalytic domains of rice (Oryza sativa) CesA8 cellulose synthase form dimers reversibly as the fundamental scaffold units of architecture in the synthase complex. Specificity of binding to UDP and UDP-Glc indicates a properly folded protein, and binding kinetics indicate that each monomer independently synthesizes single glucan chains of cellulose, i.e., two chains per dimer pair. In contrast to structure modeling predictions, solution x-ray scattering studies demonstrate that the monomer is a two-domain, elongatedmore » structure, with the smaller domain coupling two monomers into a dimer. The catalytic core of the monomer is accommodated only near its center, with the plant-specific sequences occupying the small domain and an extension distal to the catalytic domain. This configuration is in stark contrast to the domain organization obtained in predicted structures of plant CesA. As a result, the arrangement of the catalytic domain within the CesA monomer and dimer provides a foundation for constructing structural models of the synthase complex and defining the relationship between the rosette structure and the cellulose microfibrils they synthesize.« less

  6. Functional properties and structural characterization of rice δ1-pyrroline-5-carboxylate reductase

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Forlani, Giuseppe; Bertazzini, Michele; Zarattini, Marco; Funck, Dietmar; Ruszkowski, Milosz; Nocek, Bogusław

    2015-07-28

    The majority of plant species accumulate high intracellular levels of proline to cope with hyperosmotic stress conditions. Proline synthesis from glutamate is tightly regulated at both the transcriptional and the translational levels, yet little is known about the mechanisms for post-translational regulation of the enzymatic activities involved. The gene coding in rice (Oryza sativa L.) for δ1-pyrroline-5-carboxylate (P5C) reductase, the enzyme that catalyzes the second and final step in this pathway, was isolated and expressed in Escherichia coli. The structural and functional properties of the affinity-purified protein were characterized. As for most species, rice P5C reductase was able to usemore » in vitro either NADH or NADPH as the electron donor. However, strikingly different effects of cations and anions were found depending on the pyridine nucleotide used, namely inhibition of NADH-dependent activity and stimulation of NADPH-dependent activity. Moreover, physiological concentrations of proline and NADP+ were strongly inhibitory for the NADH-dependent reaction, whereas the NADPH-dependent activity was mildly affected. Our results suggest that only NADPH may be used in vivo and that stress-dependent variations in ion homeostasis and NADPH/NADP+ ratio could modulate enzyme activity, being functional in promoting proline accumulation and potentially also adjusting NADPH consumption during the defense against hyperosmotic stress. The apparent molecular weight of the native protein observed in size exclusion chromatography indicated a high oligomerization state. We also report the first crystal structure of a plant P5C reductase at 3.40-Å resolution, showing a decameric quaternary assembly. It was possible to identify dynamic structural differences among rice, human, and bacterial enzymes.« less

  7. Functional properties and structural characterization of rice ?1-pyrroline-5-carboxylate reductase

    SciTech Connect (OSTI)

    Forlani, Giuseppe; Bertazzini, Michele; Zarattini, Marco; Funck, Dietmar; Ruszkowski, Milosz; Nocek, Bogus?aw

    2015-07-28

    The majority of plant species accumulate high intracellular levels of proline to cope with hyperosmotic stress conditions. Proline synthesis from glutamate is tightly regulated at both the transcriptional and the translational levels, yet little is known about the mechanisms for post-translational regulation of the enzymatic activities involved. The gene coding in rice (Oryza sativa L.) for ?1-pyrroline-5-carboxylate (P5C) reductase, the enzyme that catalyzes the second and final step in this pathway, was isolated and expressed in Escherichia coli. The structural and functional properties of the affinity-purified protein were characterized. As for most species, rice P5C reductase was able to use in vitro either NADH or NADPH as the electron donor. However, strikingly different effects of cations and anions were found depending on the pyridine nucleotide used, namely inhibition of NADH-dependent activity and stimulation of NADPH-dependent activity. Moreover, physiological concentrations of proline and NADP+ were strongly inhibitory for the NADH-dependent reaction, whereas the NADPH-dependent activity was mildly affected. Our results suggest that only NADPH may be used in vivo and that stress-dependent variations in ion homeostasis and NADPH/NADP+ ratio could modulate enzyme activity, being functional in promoting proline accumulation and potentially also adjusting NADPH consumption during the defense against hyperosmotic stress. The apparent molecular weight of the native protein observed in size exclusion chromatography indicated a high oligomerization state. We also report the first crystal structure of a plant P5C reductase at 3.40- resolution, showing a decameric quaternary assembly. It was possible to identify dynamic structural differences among rice, human, and bacterial enzymes.

  8. Coal fly ash and phospho-gypsum mixture as an amendment to improve rice paddy soil fertility

    SciTech Connect (OSTI)

    Lee, Y.B.; Ha, H.S.; Lee, C.H.; Kim, P.J.

    2008-04-15

    Rice is a plant that requires high levels of silica (Si). As a silicate NOD source to rice, coal fly ash (hereafter, fly ash), which has an alkaline pH and high available silicate and boron (B) contents, was mixed with phosphor-gypsum (hereafter, gypsum, 50%, wt wt{sup -1}), a by-product from the production of phosphate fertilizer, to improve the fly ash limitation. Field experiments were carried out to evaluate the effect of the mixture on soil properties and rice (Oryza sativa) productivity in silt loam (SiL) and loamy sand (LS) soils to which 0 (FG 0), 20 (FG 20), 40 (FG 40), and 60 (FG 60) Mg ha{sup -1} were added. The mixture increased the amount of available silicate and exchangeable calcium (Ca) contents in the soils and the uptake of silicate by rice plant. The mixture did not result in accumulation of heavy metals in soil and an excessive uptake of heavy metals by the rice grain. The available boron content in soil increased with the mixture application levels up to 1.42 mg kg{sup -1} following the application of 60 Mg ha{sup -1} but did not show toxicity. The mixture increased significantly rice yield and showed the highest yields following the addition of 30-40 Mg ha{sup -1} in two soils. It is concluded that the fly ash and gypsum mixture could be a good source of inorganic soil amendments to restore the soil nutrient balance in rice paddy soil.

  9. Root-Uptake of C-14 Acetic Acid by Various Plants and C-14 Dynamics Surrounding the Experimental Tessera

    SciTech Connect (OSTI)

    Ogiyama, S.; Takeda, H.; Uchida, S.; Suzuki, H.; Inubushi, K.

    2008-07-01

    Carbon-14 (C-14, t{sub 1/2} = 5.73x10{sup 3} yrs) from radioactive waste is one of the most important radioactive nuclides for environmental assessment in the context of geological disposal, and understanding the transfer of radioactive elements to plants is essential for public health safety. In order to obtain fundamental knowledge, culture experiments using marigold (Tagetes patula L.), tall fescue (Festuca arundinacea S.), paddy rice (Oryza sativa L.), radish (Raphanus sativus L.), and carrot (Daucus carota L.) plants were conducted to examine root-uptake and dynamics of C-14 in the laboratory. The C-14 radioactivity in each plant part (e.g. shoot, root, edible part, etc.), medium (e.g. culture solution, sand, etc.), and air was determined. The distribution of C-14 in the plants was visualized using autoradiography. For a comparison, autoradiography was also done using Na-22. Results of the present study indicated that C-14 labeled CO{sub 2} gas was released from the culture solution to the atmosphere. Clear autoradiography images were observed in plants for the shoots and lower roots which were soaked in the culture solution. The upper roots which were not soaked in the culture solution were not clearly imaged. In the radiotracer experiment using Na-22, a clear image was observed for the whole carrot seedling, even including the upper root, on the autoradiography. However, the amounts of C-14 acetic acid absorbed by all the plants through their roots were considered to be very small. Inorganic carbon transformed from C-14 acetic acid would be taken up by plants through the roots, and some fraction of C-14 would be assimilated into the shoots by photosynthesis. (authors)

  10. SUB-MILLIMETER TELESCOPE CO (2-1) OBSERVATIONS OF NEARBY STAR-FORMING GALAXIES

    SciTech Connect (OSTI)

    Jiang, Xue-Jian; Gu, Qiusheng; Wang, Zhong; Wang, Junzhi; Zhang, Zhi-Yu

    2015-01-20

    We present CO J = 2-1 observations toward 32 nearby gas-rich star-forming galaxies selected from the ALFALFA and Wide-field Infrared Survey Explorer (WISE) catalogs, using the Sub-millimeter Telescope (SMT). Our sample is selected to be dominated by intermediate-M {sub *} galaxies. The scaling relations between molecular gas, atomic gas, and galactic properties (stellar mass, NUV – r, and WISE color W3 – W2) are examined and discussed. Our results show the following. (1) In the galaxies with stellar mass M {sub *} ≤10{sup 10} M {sub ☉}, the H I fraction (f {sub H} {sub I} ≡ M {sub H} {sub I}/M {sub *}) is significantly higher than that of more massive galaxies, while the H{sub 2} gas fraction (f{sub H{sub 2}} ≡ M{sub H{sub 2}}/M {sub *}) remains nearly unchanged. (2) Compared to f{sub H{sub 2}}, f {sub H} {sub I} correlates better with both M {sub *} and NUV – r. (3) A new parameter, WISE color W3 – W2 (12-4.6 μm), is introduced, which is similar to NUV – r in tracing star formation activity, and we find that W3 – W2 has a tighter anti-correlation with log f{sub H{sub 2}} than the anti-correlation of (NUV – r)-f {sub H} {sub I}, (NUV – r)-f{sub H{sub 2}}, and (W3 – W2)-f {sub H} {sub I}. This indicates that W3 – W2 can trace the H{sub 2} fraction in galaxies. For the gas ratio M{sub H{sub 2}}/M {sub H} {sub I} , only in the intermediate-M {sub *} galaxies it appears to depend on M {sub *} and NUV – r. We find a tight correlation between the molecular gas mass M{sub H{sub 2}} and 12 μm (W3) luminosities (L {sub 12} {sub μm}), and the slope is close to unity (1.03 ± 0.06) for the SMT sample. This correlation may reflect that the cold gas and dust are well mixed on a global galactic scale. Using the all-sky 12 μm (W3) data available in WISE, this correlation can be used to estimate CO flux for molecular gas observations and can even predict H{sub 2} mass for star-forming galaxies.

  11. TASK 3.4--IMPACTS OF COFIRING BIOMASS WITH FOSSIL FUELS

    SciTech Connect (OSTI)

    Christopher J. Zygarlicke; Donald P. McCollor; Kurt E. Eylands; Melanie D. Hetland; Mark A. Musich; Charlene R. Crocker; Jonas Dahl; Stacie Laducer

    2001-08-01

    With a major worldwide effort now ongoing to reduce greenhouse gas emissions, cofiring of renewable biomass fuels at conventional coal-fired utilities is seen as one of the lower-cost options to achieve such reductions. The Energy & Environmental Research Center has undertaken a fundamental study to address the viability of cofiring biomass with coal in a pulverized coal (pc)-fired boiler for power production. Wheat straw, alfalfa stems, and hybrid poplar were selected as candidate biomass materials for blending at a 20 wt% level with an Illinois bituminous coal and an Absaloka subbituminous coal. The biomass materials were found to be easily processed by shredding and pulverizing to a size suitable for cofiring with pc in a bench-scale downfired furnace. A literature investigation was undertaken on mineral uptake and storage by plants considered for biomass cofiring in order to understand the modes of occurrence of inorganic elements in plant matter. Sixteen essential elements, C, H, O, N, P, K, Ca, Mg, S, Zn, Cu, Fe, Mn, B, Mo, and Cl, are found throughout plants. The predominant inorganic elements are K and Ca, which are essential to the function of all plant cells and will, therefore, be evenly distributed throughout the nonreproductive, aerial portions of herbaceous biomass. Some inorganic constituents, e.g., N, P, Ca, and Cl, are organically associated and incorporated into the structure of the plant. Cell vacuoles are the repository for excess ions in the plant. Minerals deposited in these ubiquitous organelles are expected to be most easily leached from dry material. Other elements may not have specific functions within the plant, but are nevertheless absorbed and fill a need, such as silica. Other elements, such as Na, are nonessential, but are deposited throughout the plant. Their concentration will depend entirely on extrinsic factors regulating their availability in the soil solution, i.e., moisture and soil content. Similarly, Cl content is determined less by the needs of the plant than by the availability in the soil solution; in addition to occurring naturally, Cl is present in excess as the anion complement in K fertilizer applications. An analysis was performed on existing data for switchgrass samples from ten different farms in the south-central portion of Iowa, with the goal of determining correlations between switchgrass elemental composition and geographical and seasonal changes so as to identify factors that influence the elemental composition of biomass. The most important factors in determining levels of various chemical compounds were found to be seasonal and geographical differences related to soil conditions. Combustion testing was performed to obtain deposits typical of boiler fouling and slagging conditions as well as fly ash. Analysis methods using computer-controlled scanning electron microscopy and chemical fractionation were applied to determine the composition and association of inorganic materials in the biomass samples. Modified sample preparation techniques and mineral quantification procedures using cluster analysis were developed to characterize the inorganic material in these samples. Each of the biomass types exhibited different inorganic associations in the fuel as well as in the deposits and fly ash. Morphological analyses of the wheat straw show elongated 10-30-{micro}m amorphous silica particles or phytoliths in the wheat straw structure. Alkali such as potassium, calcium, and sodium is organically bound and dispersed in the organic structure of the biomass materials. Combustion test results showed that the blends fed quite evenly, with good burnout. Significant slag deposit formation was observed for the 100% wheat straw, compared to bituminous and subbituminous coals burned under similar conditions. Although growing rapidly, the fouling deposits of the biomass and coal-biomass blends were significantly weaker than those of the coals. Fouling was only slightly worse for the 100% wheat straw fuel compared to the coals. The wheat straw ash was found to show the greatest similarity from the fuel to the ash analyzed. A high percentage of particles from both fuel and ash samples contained both Si and K. While Cl was a significant component in the fuel, very little was detected in the ash sample.