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Sample records for agri culture forestry

  1. Agri Source Fuels | Open Energy Information

    Open Energy Info (EERE)

    search Name: Agri-Source Fuels Place: Pensacola, Florida Zip: 32505 Product: Biodiesel producer located in Florida that owns a plant in Dade City. References: Agri-Source...

  2. Agri Energy Inc | Open Energy Information

    Open Energy Info (EERE)

    search Name: Agri-Energy Inc Place: Nashville, Tennessee Zip: 37201 Product: Biodiesel producer, located in Nashville, Tennessee. References: Agri-Energy Inc1 This...

  3. Sunrise Agri Fuels | Open Energy Information

    Open Energy Info (EERE)

    Agri Fuels Jump to: navigation, search Name: Sunrise Agri Fuels Place: Bird Island, Minnesota Zip: 55310 Sector: Biomass Product: Manufacturer of Biomass Fuel Pellets for Pellet...

  4. East Kansas Agri Energy | Open Energy Information

    Open Energy Info (EERE)

    Kansas Agri Energy Jump to: navigation, search Name: East Kansas Agri-Energy Place: Garnett, Kansas Zip: 66032 Product: Dry-mill bioethanol producer Coordinates: 32.609607,...

  5. American Agri diesel LLC | Open Energy Information

    Open Energy Info (EERE)

    American Agri diesel LLC Jump to: navigation, search Name: American Agri-diesel LLC Place: Colorado Springs, Colorado Product: Biodiesel producer in Colorado. References: American...

  6. AgriFuel Company | Open Energy Information

    Open Energy Info (EERE)

    to: navigation, search Name: AgriFuel Company Place: Cranford, New Jersey Sector: Biofuels Product: AgriFuel produces and markets biofuels refined from waste vegetable oil,...

  7. Forestry | Open Energy Information

    Open Energy Info (EERE)

    Forestry Jump to: navigation, search Forestry is "the science of planting and caring for forests and the management of growing timber." References Retrieved from "http:...

  8. Agri Energy LLC | Open Energy Information

    Open Energy Info (EERE)

    Name: Agri-Energy LLC Place: Luverne, Minnesota Zip: 56156 Product: Corn trader and bioethanol producer. References: Agri-Energy LLC1 This article is a stub. You can help OpenEI...

  9. Commonwealth AgriEnergy | Open Energy Information

    Open Energy Info (EERE)

    Name: Commonwealth AgriEnergy Place: Hopkinsville, Kentucky Zip: 42241 Product: Bioethanol producer using corn as feedstock Coordinates: 36.867275, -87.487699 Show Map...

  10. Mid America Agri Products | Open Energy Information

    Open Energy Info (EERE)

    Products Jump to: navigation, search Name: Mid America Agri Products Place: Madrid, Nebraska Zip: 69150 Product: Ethanol producer located in Madrid, Nebraska. Coordinates:...

  11. East Kansas Agri-Energy, LLC

    SciTech Connect (OSTI)

    2007-12-01

    This is a combined heat and power (CHP) project profile on a 1.6 MW CHP application at East Kansas Agri-Energy, LLC in Garnett, Kansas.

  12. Agri Energy Funding Solutions | Open Energy Information

    Open Energy Info (EERE)

    Place: Omaha, Nebraska Zip: 68137-2495 Sector: Biomass, Wind energy Product: AGRI-ENERGY FUNDING SOLUTIONS is a market consultant for BioDiesel, Ethanol as well as Biomass...

  13. Agri capital GmbH | Open Energy Information

    Open Energy Info (EERE)

    capital GmbH Jump to: navigation, search Name: agri.capital GmbH Place: Muenster, North Rhine-Westphalia, Germany Zip: 48155 Product: Muenster-based agri.capital develops and...

  14. Forestry in Tanzania

    SciTech Connect (OSTI)

    Dykstra, D.P.

    1983-01-01

    Forest types and plantations, and associated forest industries are described. Forests occupy 47% of the total land area, mostly open miombo woodland dominated by Julbernardia and Brachystegia, with small areas of tropical high forest, mangroves and plantations. About 97% of the total roundwood consumed is used as fuelwood or for charcoal. Early results from village forestry programmes (partially financed by SIDA), the less successful communal village plantations, and agroforestry practices are described briefly. Education, training and the importance of wildlife are discussed.

  15. Agri Ethanol Products LLC AEPNC | Open Energy Information

    Open Energy Info (EERE)

    Ethanol Products LLC AEPNC Jump to: navigation, search Name: Agri-Ethanol Products LLC (AEPNC) Place: Raleigh, North Carolina Zip: 27615 Product: Ethanol producer and project...

  16. AgriKomp GmbH | Open Energy Information

    Open Energy Info (EERE)

    Zip: D-91732 Product: A major German and international group specializing in biogas plants. Subdidiaries France, Italy, Czech Rep, Poland References: agriKomp GmbH1 This...

  17. Center for International Forestry Research | Open Energy Information

    Open Energy Info (EERE)

    International Forestry Research Jump to: navigation, search Logo: Center for International Forestry Research Name: Center for International Forestry Research Address: Jalan CIFOR...

  18. Living with trees. Policies for forestry management in Zimbabwe

    SciTech Connect (OSTI)

    Bradley, E.P.; McNamara, K.

    1993-09-01

    This technical paper provides a broad analysis of forestry policy in Zimbabwe. Some two dozen contributors look at forestry as a provider of livelihood, homes and workplaces, and industrial commodities. The first section of the paper concentrates on the social and macroeconomic goals of forestry in Zimbabwe and on its place in rural agriculture. Contributors examine the issues of land tenure and the culture of common property, the practice of woodland management, and control of and participation in management and policy development. The second section focuses on the status of the forest industry in Zimbabwe and on its capacity to manage and expand existing commercial plantations. The paper explores the main issues the sector faces, including growing concentration of ownership versus the government`s objective of wider participation in the industry. They discuss the effects of trade liberalization on its competitive position, both among other agricultural sectors within Zimbabwe and with competitors outside the country. Included is a color map of Zimbabwe`s woodlands. Many forestry programs worldwide face the issues discussed in this work. It will be of interest to planners, policymakers, teachers, and students of rural development and forestry.

  19. Integration of Biodiversity into National Forestry Planning:...

    Open Energy Info (EERE)

    Biodiversity into National Forestry Planning: An Annotated Bibliography of Web-Based Resources, Methods, Experiences, and Case Studies Jump to: navigation, search Tool Summary...

  20. USAID-Forestry Conflict Management Training | Open Energy Information

    Open Energy Info (EERE)

    Forestry Conflict Management Training Jump to: navigation, search Tool Summary LAUNCH TOOL Name: USAID-Forestry Conflict Management Training AgencyCompany Organization: United...

  1. Hawaii Department of Land and Natural Resources Division of Forestry...

    Open Energy Info (EERE)

    of Forestry and Wildlife Jump to: navigation, search Name: Hawaii Department of Land and Natural Resources Division of Forestry and Wildlife Address: Kalanimoku Building...

  2. Utah Division of Forestry, Fire and State Lands | Open Energy...

    Open Energy Info (EERE)

    of Forestry, Fire and State Lands Address: 1594 W. North Temple, Ste 3520 Place: Salt Lake City, Utah Zip: 84114-5703 Phone Number: 801.538.5555 Website: forestry.utah.gov...

  3. Carbon Market Opportunities for the Forestry Sector of Africa...

    Open Energy Info (EERE)

    of the United Nations, Winrock International Sector: Land Focus Area: Renewable Energy, Forestry Topics: Implementation, Policiesdeployment programs Resource Type:...

  4. Before the Senate Agriculture, Nutrition, and Forestry | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Agriculture, Nutrition, and Forestry Before the Senate Agriculture, Nutrition, and Forestry Before the Senate Agriculture, Nutrition, and Forestry By: Richard Newell, Administrator Energy Information Administration Subject: Development in Energy Markets and their possible implications on Agriculture Final_Testimony(22).pdf (32.7 KB) More Documents & Publications Before the Committee on Agriculture Subcommittee on General Farm Commodities and Risk Management Hearing Before the

  5. Carbon Offsets for Forestry and Bioenergy: Researching Opportunities...

    Open Energy Info (EERE)

    Researching Opportunities for Poor Rural Communities Jump to: navigation, search Name Carbon Offsets for Forestry and Bioenergy: Researching Opportunities for Poor Rural...

  6. FORESTRY COLORADO WESTERN POWER ADMIN POC Cheryl Drake Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    FORESTRY COLORADO WESTERN POWER ADMIN POC Cheryl Drake Telephone (720) 962-7154 Email drake@wapa.gov Timber tract operations 113110 Cutting and transporting timber 113310 GEORGIA ...

  7. Global Timber Market and Forestry Data Project | Open Energy...

    Open Energy Info (EERE)

    data has been used in analysis should visit the Forests, Economics and Global Climate Change website." References "Global Timber Market and Forestry Data Project" Retrieved...

  8. Forestry-based Carbon Sequestration Projects in Africa: Potential...

    Open Energy Info (EERE)

    Abstract "Carbon sequestration through forestry and agroforestry can help mitigate global warming. For Africa, carbon sequestration also represents an opportunity to fund...

  9. Climate Change Mitigation in the Energy and Forestry Sectors...

    Open Energy Info (EERE)

    of Developing Countries Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Climate Change Mitigation in the Energy and Forestry Sectors of Developing Countries...

  10. EIS-0300: Minnesota Agri-Power Project: Biomass for Rural Development, Granite Falls, Minnesota

    Broader source: Energy.gov [DOE]

    This EIS analyzes DOE and the Minnesota Environmental Quality Boards' [MEQB, a Minnesota State agency] decision to support a proposal by the Minnesota Valley Alfalfa Producers (MnVAP) to construct and operate a 75–103 megawatt biomass fueled gasifier and electric generating facility, known as the Minnesota Agri-Power Plant (MAPP), and associated transmission lines and alfalfa processing facilities.

  11. USDA National Urban and Community Forestry Challenge Cost Share...

    Broader source: Energy.gov (indexed) [DOE]

    5 5:00PM EST U.S. Department of Agriculture The U.S. Department of Agriculture is accepting proposals for the National Urban and Community Forestry Challenge Cost Share Grant...

  12. Biomass Support for the China Renewable Energy Law: Feasibility Report -- Agricultural and Forestry Solid Wastes Power Generation Demonstration, December 2005

    SciTech Connect (OSTI)

    Not Available

    2006-10-01

    Subcontractor report on feasibility of using agricultural and forestry wastes for power generation in China

  13. Summary of current state nonpoint source control practices for forestry. Final report

    SciTech Connect (OSTI)

    Craig, J.; Parcher, M.A.; Wright, J.; Townsend, G.; Cannell, J.

    1993-08-01

    Forestry practices have been implicated by many states as a contributor to nonpoint source (NPS) pollution of streams, rivers, lakes, and other waterbodies. Many states, recognizing the forestry contribution to water pollution, have developed programs to address forestry NPS. The recent development of the Management Measures Guidance, as required by section 6217 of the Coastal Zone Reauthorization Amendments (CZARA), provided the motivation to develop a summary of the current state forestry NPS programs. The document provides a synopsis of the BMPs currently used by states to address the nonpoint source (NPS) impacts on water quality caused by forestry activities. Summaries of over 41 existing state BMP manuals or regulations that include BMPs are presented. The information presented in the document is intended to provide an increased understanding of the types of forestry activities commonly addressed in existing state NPS programs and to serve as a reference for the type and nature of BMPs included in current state BMP manuals.

  14. The role of forestry development in China in alleviating greenhouse effects

    SciTech Connect (OSTI)

    Liu Hong

    1996-12-31

    Forestry development in China has gained great achievements and made great progress in realizing sustainable forest management and alleviating global climate change. The main measures to mitigate greenhouse effects through the means of forestry development include afforestation to increase the forested area, fuel wood forest development, management improvement, wise utilization, international cooperation, investment increase, forest related scientific research, strengthening the forest law enforcement system. Climate change as well as how to alleviate the greenhouse effects is a hot topic at present. This paper describes the achievements of China`s forestry development and its role to alleviate the greenhouse effects, and puts forward the measures to mitigate greenhouse effects through the means of forestry development.

  15. Scenarios of forestry carbon sequestration measures in the Russian Federation and priorities for action plan

    SciTech Connect (OSTI)

    Kokorin, A.O.

    1996-12-31

    Development of forestry mitigation strategy under Russian transition economy conditions has many difficulties and specific features. The most important factors are: shortage in funds; absence of well defined legislation, rules and standards; absence of adequate control systems; weak transport infrastructure and export problems. Assessment of economic possibilities, potential, short- and middle-term measures show that strategies have to be focused on improvement and promotion of current carbon sequestration activity. Five baseline forestry scenario (No. 1) and four other scenarios (No. 2 - No. 5) for 2000-2040 were developed. Each scenario covers all forested area but provides separate analysis of 30 `forestry ecoregions`. Three types of forestry management were included in scenarios: clear-cut logging and reforestation (by scenarios No. 2 and No. 3); selective logging and thinning (No. 4); measures to prevent and manage fires (No. 5). The baseline scenario results in a constant net-sink of about 150 MtC/yr. An increase in clear-cut logging on the basis of current forestry practice will cause a rapid drop of net-sink. Implementation of a modest increase in clear-cut logging with active forest fire and selective logging measures could provide with a slight increase of net-sink. Consideration of scenarios helps identify regional forestry priorities for Russian Climate Change Action Plan. The priorities by region include: European-Ural: (1) creation of economy mechanism to increase forestry effectiveness on the same cutting areas, (2) assistance to natural reforestation. Central and North-East Siberia: promotion of forest fire protection system. South Siberia and Primorie and Priamurie: limit of clear-cut logging and creation market situation for better forestry efficiency. The proposed Joint Implementation Vologda reforestation project which is being considered now by special bodies of the USA and the Russian Federation is in good agreement with these priorities.

  16. Overview of mitigation policies and measures in the forestry sector

    SciTech Connect (OSTI)

    Sathaye, J.

    1996-12-31

    In this paper the author addresses questions on how the forestry sector can make a contribution to the general problem of greenhouse gases in the environment. Primarily this is in the form of carbon conservation and sequestering. There is a potential land area for conservation and sequestration estimated to be 700 Mha. The total carbon that could be sequestered and conserved globally by 2050 on this land is 60 - 87 GtC. Slowing deforestation, assisting regeneration, forestation and agroforestry are the primary mitigation measures for carbon conservation and sequestration. For long term success, enforcement to halt deforestation has to be accompained by economic and/or other benefits to the deforesters that equal or exceed their current remuneration. Making plantations a significant fuel for utility electricity generation will require higher biomass yields and thermal efficiency matching that of conventional plants. Significant reduction of global carbon emissions requires national governments to institute measures that provide local, national, economic and other benefits while conserving and sequestering carbon.

  17. Special Issue On Estimation Of Baselines And Leakage In CarbonMitigation Forestry Projects

    SciTech Connect (OSTI)

    Sathaye, Jayant A.; Andrasko, Kenneth

    2006-06-01

    There is a growing acceptance that the environmentalbenefits of forests extend beyond traditional ecological benefits andinclude the mitigation of climate change. Interest in forestry mitigationactivities has led to the inclusion of forestry practices at the projectlevel in international agreements. Climate change activities place newdemands on participating institutions to set baselines, establishadditionality, determine leakage, ensure permanence, and monitor andverify a project's greenhouse gas benefits. These issues are common toboth forestry and other types of mitigation projects. They demandempirical evidence to establish conditions under which such projects canprovide sustained long term global benefits. This Special Issue reportson papers that experiment with a range of approaches based on empiricalevidence for the setting of baselines and estimation of leakage inprojects in developing Asia and Latin America.

  18. USDA National Urban and Community Forestry Challenge Cost Share Grant Program

    Broader source: Energy.gov [DOE]

    The U.S. Department of Agriculture is accepting proposals for the National Urban and Community Forestry Challenge Cost Share Grant Program to assist the U.S. Forest Service in establishing the grant categories and recommendations of final proposals for the Forest Service to consider.

  19. Global Climate Change: Some Implications, Opportunities, and Challenges for US Forestry

    DOE R&D Accomplishments [OSTI]

    Marland, G.

    1991-06-01

    It is widely agreed that the concentration of greenhouse gases in the earth`s atmosphere is increasing, that this increase is a consequence of man`s activities, and that there is significant risk that this will lead to changes in the earth`s climate. The question is now being discussed what, if anything, we should be doing to minimize and/or adapt to changes in climate. Virtually every statement on this matter; from the US Office of Technology Assessment, to the National Academy of Science, to the Nairobi Declaration on Climatic Change, includes some recommendation for planting and protecting forests. In fact, forestry is intimately involved in the climate change debate for several reasons: changing climate patterns will affect existing forests, tropical deforestation is one of the major sources of greenhouse gases to the atmosphere, reforestation projects could remove additional carbon dioxide from the atmosphere and there is renewed interest in wood-based or other renewable fuels to replace fossil fuels. Part of the enthusiasm for forestry-related strategies in a greenhouse context is the perception that forests not only provide greenhouse benefits but also serve other desirable social objectives. This discussion will explore the current range of thinking in this area and try to stimulate additional thinking on the rationality of the forestry-based approaches and the challenges posed for US forestry.

  20. Estimating the greenhouse gas benefits of forestry projects: A Costa Rican Case Study

    SciTech Connect (OSTI)

    Busch, Christopher; Sathaye, Jayant; Sanchez Azofeifa, G. Arturo

    2000-09-01

    If the Clean Development Mechanism proposed under the Kyoto Protocol is to serve as an effective means for combating global climate change, it will depend upon reliable estimates of greenhouse gas benefits. This paper sketches the theoretical basis for estimating the greenhouse gas benefits of forestry projects and suggests lessons learned based on a case study of Costa Rica's Protected Areas Project, which is a 500,000 hectare effort to reduce deforestation and enhance reforestation. The Protected Areas Project in many senses advances the state of the art for Clean Development Mechanism-type forestry projects, as does the third-party verification work of SGS International Certification Services on the project. Nonetheless, sensitivity analysis shows that carbon benefit estimates for the project vary widely based on the imputed deforestation rate in the baseline scenario, e.g. the deforestation rate expected if the project were not implemented. This, along with a newly available national dataset that confirms other research showing a slower rate of deforestation in Costa Rica, suggests that the use of the 1979--1992 forest cover data originally as the basis for estimating carbon savings should be reconsidered. When the newly available data is substituted, carbon savings amount to 8.9 Mt (million tones) of carbon, down from the original estimate of 15.7 Mt. The primary general conclusion is that project developers should give more attention to the forecasting land use and land cover change scenarios underlying estimates of greenhouse gas benefits.

  1. Greenhouse gas mitigation options in the forestry sector of The Gambia: Analysis based on COMAP model

    SciTech Connect (OSTI)

    Jallow, B.P.

    1996-12-31

    Results of the 1993 Greenhouse Gas Emissions Inventory of The Gambia showed net CO{sub 2} emissions of over (1.66 x 10{sup 6} tons) and 1% was due to uptake by plantations (0.01 x 10{sup 6} tons). This is a clear indication that there is need to identify changes in the land-use policy, law and tenure that discourages forest clearing at the same time significantly influencing the sustainable distribution of land among forestry, rangeland and livestock, and agriculture. About 11% of the total area of The Gambia is either fallow or barren flats that once supported vegetation and hence is still capable of supporting vegetation. The US Country Study Programme has provided the Government of The Gambia through the National Climate Committee funds to conduct Assessment of Mitigation Options to Reduce Greenhouse Gas Emissions. The Forestry Sector is one area for which assessment is being conducted. The assessment is expected to end in September 1996. The Comprehensive Mitigation Analysis Process (COMAP) is one of the Models supplied to the National Climate Committee by the Lawrence Berkeley Laboratory, on behalf of the US Country Study Programme, and is being used to conduct the analysis in The Gambia.

  2. Mitigation Options in Forestry, Land-Use, Change and Biomass Burning in Africa

    SciTech Connect (OSTI)

    Makundi, Willy R.

    1998-06-01

    Mitigation options to reduce greenhouse gas emissions and sequester carbon in land use sectors are describe in some detail. The paper highlights those options in the forestry sector, which are more relevant to different parts of Africa. It briefly outlines a bottom-up methodological framework for comprehensively assessing mitigation options in land use sectors. This method emphasizes the application of end-use demand projections to construct a baseline and mitigation scenarios and explicitly addresses the carbon storage potential on land and in wood products, as well as use of wood to substitute for fossil fuels. Cost-effectiveness indicators for ranking mitigation options are proposed, including those, which account for non-carbon monetary benefits such as those derived from forest products, as well as opportunity cost of pursuing specific mitigation option. The paper finally surveys the likely policies, barriers and incentives to implement such mitigation options in African countries .

  3. Global and regional potential for bioenergy from agricultural and forestry residue biomass

    SciTech Connect (OSTI)

    Gregg, Jay S.; Smith, Steven J.

    2010-02-11

    As co-products, agricultural and forestry residues represent a potential low cost, low carbon, source for bioenergy. A method is developed method for estimating the maximum sustainable amount of energy potentially available from agricultural and forestry residues by converting crop production statistics into associated residue, while allocating some of this resource to remain on the field to mitigate erosion and maintain soil nutrients. Currently, we estimate that the world produces residue biomass that could be sustainably harvested and converted into over 50 EJ yr-1 of energy. The top three countries where this resource is estimated to be most abundant are currently net energy importers: China, the United States (US), and India. The global potential from residue biomass is estimated to increase to approximately 80-95 EJ yr-1 by mid- to late- century, depending on physical assumptions such as of future crop yields and the amount of residue sustainably harvestable. The future market for biomass residues was simulated using the Object-Oriented Energy, Climate, and Technology Systems Mini Climate Assessment Model (ObjECTS MiniCAM). Utilization of residue biomass as an energy source is projected for the next century under different climate policy scenarios. Total global use of residue biomass is estimated to increase to 70-100 EJ yr-1 by mid- to late- century in a central case, depending on the presence of a climate policy and the economics of harvesting, aggregating, and transporting residue. Much of this potential is in developing regions of the world, including China, Latin America, Southeast Asia, and India.

  4. Safeguards Culture

    SciTech Connect (OSTI)

    Frazar, Sarah L.; Mladineo, Stephen V.

    2012-07-01

    The concepts of nuclear safety and security culture are well established; however, a common understanding of safeguards culture is not internationally recognized. Supported by the National Nuclear Security Administration, the authors prepared this report, an analysis of the concept of safeguards culture, and gauged its value to the safeguards community. The authors explored distinctions between safeguards culture, safeguards compliance, and safeguards performance, and evaluated synergies and differences between safeguards culture and safety/security culture. The report concludes with suggested next steps.

  5. GHG Mitigation potential and cost in tropical forestry - Relative role for agroforestry

    SciTech Connect (OSTI)

    Makundi, Willy R.; Sathaye, Jayant A.

    2004-01-01

    This paper summarizes studies of carbon mitigation potential (MP) and costs of forestry options in seven developing countries with a focus on the role of agroforestry. A common methodological approach known as comprehensive mitigation assessment process (COMAP) was used in each study to estimate the potential and costs between 2000 and 2030. The approach requires the projection of baseline and mitigation land-use scenarios derived from the demand for forest products and forestland for other uses such as agriculture and pasture. By using data on estimated carbon sequestration, emission avoidance, costs and benefits, the model enables one to estimate cost effectiveness indicators based on monetary benefit per t C, as well as estimates of total mitigation costs and potential when the activities are implemented at equilibrium level. The results show that about half the MP of 6.9 Gt C (an average of 223 Mt C per year) between 2000 and 2030 in the seven countries could be achieved at a negative cost, and the other half at costs not exceeding $100 per t C. Negative cost indicates that non-carbon revenue is sufficient to offset direct costs of about half of the options. The agroforestry options analyzed bear a significant proportion of the potential at medium to low cost per t C when compared to other options. The role of agroforestry in these countries varied between 6% and 21% of the MP, though the options are much more cost effective than most due to the low wage or opportunity cost of rural labor. Agroforestry options are attractive due to the large number of people and potential area currently engaged in agriculture, but they pose unique challenges for carbon and cost accounting due to the dispersed nature of agricultural activities in the tropics, as well as specific difficulties arising from requirements for monitoring, verification, leakage assessment and the establishment of credible baselines.

  6. Mitigation potential and cost in tropical forestry - relative role for agroforestry

    SciTech Connect (OSTI)

    Makundi, Willy R.; Sathaye, Jayant A.

    2004-01-01

    This paper summarizes studies of carbon mitigation potential (MP) and costs of forestry options in seven developing countries with a focus on the role of agroforestry. A common methodological approach known as comprehensive mitigation assessment process (COMAP) was used in each study to estimate the potential and costs between 2000 and 2030. The approach requires the projection of baseline and mitigation land-use scenarios derived from the demand for forest products and forestland for other uses such as agriculture and pasture. By using data on estimated carbon sequestration, emission avoidance, costs and benefits, the model enables one to estimate cost effectiveness indicators based on monetary benefit per t C, as well as estimates of total mitigation costs and potential when the activities are implemented at equilibrium level. The results show that about half the MP of 6.9 Gt C (an average of 223 Mt C per year) between 2000 and 2030 in the seven countries could be achieved at a negative cost, and the other half at costs not exceeding $100 per t C. Negative cost indicates that non-carbon revenue is sufficient to offset direct costs of about half of the options. The agroforestry options analyzed bear a significant proportion of the potential at medium to low cost per t C when compared to other options. The role of agroforestry in these countries varied between 6% and 21% of the MP, though the options are much more cost effective than most due to the low wage or opportunity cost of rural labor. Agroforestry options are attractive due to the large number of people and potential area currently engaged in agriculture, but they pose unique challenges for carbon and cost accounting due to the dispersed nature of agricultural activities in the tropics, as well as specific difficulties arising from requirements for monitoring, verification, leakage assessment and the establishment of credible baselines.

  7. Forestry herbicide influences on biodiversity and wildlife habitats in Southern forests.

    SciTech Connect (OSTI)

    Miller, Karl V.

    2004-01-01

    Abstract In the southern United States, herbicide use continues to increase for timber management in commercial pine (Pinus spp.) plantations, for modifying wildlife habitats, and for invasive plant control. Several studies have reported that single applications of forestry herbicides at stand initiation have minor and temporary impacts on plant communities and wildlife habitat conditions, with some reports of enhanced habitat conditions for both game and nongame species. Due to the high resiliency of floral communities, plant species richness and diversity rebound rapidly after single herbicide treatments, with short- and long-term compositional shifts according to the selectivity and efficacy of the herbicide used. Recently, however, a shift to the Southeast in North American timber supplies has resulted in increased forest management intensity. Current site-preparation techniques rely on herbicide combinations, often coupled with mechanical treatments and >1 years of post-planting applications to enhance the spectrum and duration of vegetation control. This near-total control of associated vegetation at establishment and more rapid pine canopy closure, coupled with shortened and repeated rotations, likely will affect plant diversity and wildlife habitat quality. Development of mitigation methods at the stand and landscape levels will be required to minimize vegetative and wildlife impacts while allowing continued improvement in pine productivity. More uncertain are long-term impacts of increasing invasive plant occupation and the projected increase in herbicide use that will be needed to reverse this worsening situation. In addition, the potential of herbicides to meet wildlife management objectives in areas where traditional techniques have high social costs (e.g., prescribed fire) should be fully explored.

  8. Forestry herbicide influences on biodiversity and wildlife habitat in Southern forests.

    SciTech Connect (OSTI)

    Miller, Karl V.; Miller, James, H.

    2004-07-01

    Abstract In the southern United States, herbicide use continues to increase for timber management in commercial pine (Pinus spp.) plantations, for modifying wildlife habitats, and for invasive plant control. Several studies have reported that single applications of forestry herbicides at stand initiation have minor and temporary impacts on plant communities and wildlife habitat conditions, with some reports of enhanced habitat conditions for both game and nongame species. Due to the high resiliency of floral communities, plant species richness and diversity rebound rapidly after single herbicide treatments, with short- and long-term compositional shifts according to the selectivity and efficacy of the herbicide used. Recently, however, a shift to the Southeast in North American timber supplies has resulted in increased forest management intensity. Current site-preparation techniques rely on herbicide combinations, often coupled with mechanical treatments and >1 years of post-planting applications to enhance the spectrum and duration of vegetation control. This near-total control of associated vegetation at establishment and more rapid pine canopy closure, coupled with shortened and repeated rotations, likely will affect plant diversity and wildlife habitat quality. Development of mitigation methods at the stand and landscape levels will be required to minimize vegetative and wildlife impacts while allowing continued improvement in pine productivity. More uncertain are long-term impacts of increasing invasive plant occupation and the projected increase in herbicide use that will be needed to reverse this worsening situation. In addition, the potential of herbicides to meet wildlife management objectives in areas where traditional techniques have high social costs (e.g., prescribed fire) should be fully explored.

  9. Cultural Preservation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    environmental protection program, LANL specialists oversee and manage the Laboratory's cultural resources programs. Several laws, including the National Historic Preservation...

  10. I think that I shall never see {hor_ellipsis} a lovely forestry policy: Land use programs for conservation of forests

    SciTech Connect (OSTI)

    Rayner, S.F.; Richards, K.R.

    1994-01-01

    Forestry programs are frequently invoked as having potential for mitigation of greenhouse gas emissions. Most studies have attempted to quantify the potential impact of forest programs on carbon uptake and the potential costs of such programs. In this paper, we will attempt instead to focus on the institutional issues of the implementation of forestry programs for carbon sequestration. In particular, we explore the challenges for implementing forest programs that are: of increasing technological complexity; and in settings that depart significantly from the idealized conditions of economic models. We start in Section 1 by examining a suite of instruments that are commonly employed to implement a given policy. Section 2 examines a relatively simple case -- a tree-planting program in the US -- and demonstrates that there are significant difficulties involved in implementing a carbon sequestration program, even in a well-developed market economy. Section 3 focuses on other technologies in the US and why the choice of policy instruments and program design is more difficult than for the simple tree-planting case. Section 4 considers implementation of forestry policies in other countries where the economies may bear less resemblance to the ideal market economy than the US. In those settings, the choice of policy instruments may be very sensitive to non-market considerations that are often missed in conventional policy and cost analysis.

  11. Carbon mitigation potential and costs of forestry options in Brazil, China, India, Indonesia, Mexico, the Philippines and Tanzania

    SciTech Connect (OSTI)

    Sathaye, J.; Makundi, W.; Andrasko, K.; Boer, R.; Ravindranath, N.; Sudha, P.; Rao, S.; Lasco, R.; Pulhin, F.; Masera, O.; Ceron, A.; Ordonez, J.; Deying, X.; Zhang, X.; Zuomin, S.

    2001-01-01

    This paper summarizes studies of carbon (C) mitigation potential and costs of about 40 forestry options in seven developing countries. Each study uses the same methodological approach - Comprehensive Mitigation Assessment Process (COMAP) - to estimate the above parameters between 2000 and 2030. The approach requires the projection of baseline and mitigation land-use scenarios. Coupled with data on a per ha basis on C sequestration or avoidance, and costs and benefits, it allows the estimation of monetary benefit per Mg C, and the total costs and carbon potential. The results show that about half (3.0 Pg C) the cumulative mitigation potential of 6.2 Petagram (Pg) C between 2000 and 2030 in the seven countries (about 200 x 106 Mg C yr-1) could be achieved at a negative cost and the remainder at costs ranging up to $100 Mg C-1. About 5 Pg C could be achieved, at a cost less than $20 per Mg C. Negative cost potential indicates that non-carbon revenue is sufficient to offset direct costs of these options. The achievable potential is likely to be smaller, however, due to market, institutional, and sociocultural barriers that can delay or prevent the implementation of the analyzed options.

  12. Analysis Of Leakage In Carbon Sequestration Projects In Forestry:A Case Study Of Upper Magat Watershed, Philippines

    SciTech Connect (OSTI)

    Lasco, Rodel D.; Pulhin, Florencia B.; Sales, Renezita F.

    2007-06-01

    The role of forestry projects in carbon conservation andsequestration is receiving much attention because of their role in themitigation of climate change. The main objective of the study is toanalyze the potential of the Upper Magat Watershed for a carbonsequestration project. The three main development components of theproject are forest conservation, tree plantations, and agroforestry farmdevelopment. At Year 30, the watershed can attain a net carbon benefit of19.5 M tC at a cost of US$ 34.5 M. The potential leakage of the projectis estimated using historical experience in technology adoption inwatershed areas in the Philippines and a high adoption rate. Two leakagescenarios were used: baseline and project leakage scenarios. Most of theleakage occurs in the first 10 years of the project as displacement oflivelihood occurs during this time. The carbon lost via leakage isestimated to be 3.7 M tC in the historical adoption scenario, and 8.1 MtC under the enhanced adoption scenario.

  13. Anaerobic thermophilic culture

    DOE Patents [OSTI]

    Ljungdahl, Lars G.; Wiegel, Jurgen K. W.

    1981-01-01

    A newly discovered thermophilic anaerobe is described that was isolated in a biologically pure culture and designated Thermoanaerobacter ethanolicus ATCC 3/550. T. Ethanolicus is cultured in aqueous nutrient medium under anaerobic, thermophilic conditions and is used in a novel process for producing ethanol by subjecting carbohydrates, particularly the saccharides, to fermentation action of the new microorganism in a biologically pure culture.

  14. Safeguards Culture: Lessons Learned

    SciTech Connect (OSTI)

    Frazar, Sarah L.; Mladineo, Stephen V.

    2010-06-01

    Today, safeguards culture can be a useful tool for measuring nonproliferation postures, but so far its impact on the international safeguards regime has been underappreciated. There is no agreed upon definition for safeguards culture nor agreement on how it should be measured. This paper argues that safeguards culture as an indicator of a countrys nonproliferation posture can be a useful tool.

  15. Safeguards Culture: Lessons Learned

    SciTech Connect (OSTI)

    Mladineo, Stephen V.

    2009-05-27

    Abstract: At the 2005 INMM/ESARDA Workshop in Santa Fe, New Mexico, I presented a paper entitled Changing the Safeguards Culture: Broader Perspectives and Challenges. That paper described a set of theoretical models that can be used as a basis for evaluating changes to safeguards culture. This paper builds on that theoretical discussion to address practical methods for influencing culture. It takes lessons from methods used to influence change in safety culture and security culture, and examines the applicability of these lessons to changing safeguards culture. Paper: At the 2005 INMM/ESARDA Workshop on Changing the Safeguards Culture: Broader Perspectives and Challenges, in Santa Fe, New Mexico, I presented a paper entitled Changing the Safeguards Culture: Broader Perspectives and Challenges. That paper, coauthored by Karyn R. Durbin and Andrew Van Duzer, described a set of theoretical models that can be used as a basis for evaluating changes to safeguards culture. This paper updates that theoretical discussion, and seeks to address practical methods for influencing culture. It takes lessons from methods used to influence change in safety culture and security culture, and examines the applicability of these lessons to changing safeguards culture. Implicit in this discussion is an understanding that improving a culture is not an end in itself, but is one method of improving the underlying discipline, that is safety, security, or safeguards. Culture can be defined as a way of life, or general customs and beliefs of a particular group of people at a particular time. There are internationally accepted definitions of safety culture and nuclear security culture. As yet, there is no official agreed upon definition of safeguards culture. At the end of the paper I will propose my definition. At the Santa Fe Workshop the summary by the Co-Chairs of Working Group 1, The Further Evolution of Safeguards, noted: It is clear that safeguards culture needs to be

  16. ORISE: Our Culture

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Our Culture The Oak Ridge Institute for Science and Education (ORISE) cares about its employees, and this is evident through the abundance of benefits-both tangible and...

  17. Anaerobic thermophilic culture system

    DOE Patents [OSTI]

    Ljungdahl, Lars G.; Wiegel, Jurgen K. W.

    1981-01-01

    A mixed culture system of the newly discovered microorganism Thermoanaerobacter ethanolicus ATCC31550 and the microorganism Clostridium thermocellum ATCC31549 is described. In a mixed nutrient culture medium that contains cellulose, these microorganisms have been coupled and cultivated to efficiently ferment cellulose to produce recoverable quantities of ethanol under anaerobic, thermophilic conditions.

  18. Bootheel Agri Energy | Open Energy Information

    Open Energy Info (EERE)

    Zip: 63801 Product: Developer of a now-postponed 100m gallon (378m litre) per year bioethanol plant in Sikeston, Missouri. Coordinates: 36.876525, -89.588284 Show Map Loading...

  19. Safety Culture in Nuclear Installations

    Broader source: Energy.gov [DOE]

    IAEA-TECDOC-1329 Safety Culture in Nuclear Installations, Guidance for use in the Enhancement of Safety Culture, International Atomic Energy Agency IAEA, December 2002.

  20. Hanford Cultural Resources - Hanford Site

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    About Us Hanford Site Wide Programs Hanford's Tribal Program Hanford Cultural Resources About Us Hanford's Tribal Program Home Hanford Cultural Resources DOE American Indian Tribal...

  1. Mass algal culture system

    DOE Patents [OSTI]

    Raymond, Lawrence P.

    1981-01-01

    An apparatus and process for the culture of algae in a liquid medium is disclosed. The medium circulates through an open trough and is exposed to an atmosphere which is temperature regulated. The nutrient content of the liquid medium is regulated to control the chemical composition growth and reproduction characteristics of the cultured algae. Before it is allowed to strike the medium, sunlight is passed through a filter to remove wavelengths which are not photosynthetically active. Heat energy can be recovered from the filter.

  2. Mass algal culture system

    DOE Patents [OSTI]

    Raymond, Lawrence P.

    1982-01-01

    An apparatus and process for the culture of algae in a liquid medium is disclosed. The medium circulates through an open trough and is exposed to an atmosphere which is temperature regulated. The nutrient content of the liquid medium is regulated to control the chemical composition growth and reproduction characteristics of the cultured algae. Before it is allowed to strike the medium, sunlight is passed through a filter to remove wavelengths which are not photosynthetically active. Heat energy can be recovered from the filter.

  3. Measuring Safeguards Culture

    SciTech Connect (OSTI)

    Frazar, Sarah L.; Mladineo, Stephen V.

    2011-07-19

    As the International Atomic Energy Agency (IAEA) implements a State Level Approach to its safeguards verification responsibilities, a number of countries are beginning new nuclear power programs and building new nuclear fuel cycle faculties. The State Level approach is holistic and investigatory in nature, creating a need for transparent, non-discriminatory judgments about a state's nonproliferation posture. In support of this need, the authors previously explored the value of defining and measuring a state's safeguards culture. We argued that a clear definition of safeguards culture and an accompanying set of metrics could be applied to provide an objective evaluation and demonstration of a country's nonproliferation posture. As part of this research, we outlined four high-level metrics that could be used to evaluate a state's nuclear posture. We identified general data points. This paper elaborates on those metrics, further refining the data points to generate a measurable scale of safeguards cultures. We believe that this work could advance the IAEA's goals of implementing a safeguards system that is fully information driven, while strengthening confidence in its safeguards conclusions.

  4. DOE - NNSA/NFO -- Cultural Resources

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cultural Resources NNSANFO Language Options U.S. DOENNSA - Nevada Field Office Click to subscribe to NNSS News Cultural Resources Environmental Cultural photo Prehistoric ...

  5. Poeh Cultural Center wins grant

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Poeh Cultural Center wins grant, educates public Community Connections: Your link to news and opportunities from Los Alamos National Laboratory Latest Issue: September 1, 2016 all issues All Issues » submit Poeh Cultural Center wins grant Native American Venture Acceleration Fund money helps increase education and tourism. May 2, 2016 Poeh Cultural Center and Museum received a grant through the Native American Venture Acceleration Fund in January to develop training programs for artists and to

  6. Cultural Resources | Open Energy Information

    Open Energy Info (EERE)

    source History View New Pages Recent Changes All Special Pages Semantic SearchQuerying Get Involved Help Apps Datasets Community Login | Sign Up Search Page Edit History Cultural...

  7. Surveys of organizational culture and safety culture in nuclear power

    SciTech Connect (OSTI)

    Brown, Walter S.

    2000-07-30

    The results of a survey of organizational culture at a nuclear power plant are summarized and compared with those of a similar survey which has been described in the literature on ''high-reliability organizations''. A general-purpose cultural inventory showed a profile of organizational style similar to that reported in the literature; the factor structure for the styles was also similar to that of the plant previously described. A specialized scale designed to measure ''safety culture'' did not distinguished among groups within the organization that would be expected to differ.

  8. Organizational Culture and Safety Culture: Are they one and the...

    Office of Environmental Management (EM)

    upward information flow won't exist in the safety aspect for very long. If employee involvement in planning is not a norm for the organizational culture - employee involvement in...

  9. Hanford cultural resources management plan

    SciTech Connect (OSTI)

    Chatters, J.C.

    1989-06-01

    As a federal agency, the US Department of Energy (DOE) has been directed by Congress and the President to provide leadership in the preservation of prehistoric, historical, and cultural resources on lands it administers, to manage these in a spirit of stewardship for future generations, and to protect and preserve the rights of Native Americans to religious freedom. The purpose of this document is to describe how the DOE-Richland Operations (DOE-RL) will meet those responsibilities on the Hanford Site, pursuant to guidelines for Agency Responsibilities under the Historic Preservation Act (FR 53:31, February 17, 1988). This document is intended for multiple uses. Among other things, the text is designed as a manual for cultural resource managers to follow and as an explanation of the process of cultural resource regulatory compliance for the DOE-RL and Site contractors. 10 refs., 17 figs., 11 tabs.

  10. Perspectives on Changing Safeguards Culture

    SciTech Connect (OSTI)

    Mladineo, Stephen V.; Durbin, Karyn R.; Van Duzer, Andrew

    2005-12-01

    The importance of culture in the nuclear field has become widely recognized. In the wake of the 9-11 attacks in the United States, and terrorist attacks worldwide, the international community has become interested in strengthening nuclear security culture for much of the same reasons that it became interested in strengthening the nuclear safety culture in the 1980s. The accidents that occurred at Three Mile Island and Chernobyl led to a realization that nuclear operations in one country can directly affect other countries. The accidents also led to the realization that technology alone cannot guarantee safety and that the human element has a key role to play in the safety operation of nuclear power plans.

  11. Introduction to Safety Culture Advice

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Safety Culture Advice Thursday, June 7, 2012 As expressed in past advice, and in draft advice being proposed today, the safe and effective treatment of Hanford's tank waste through vitrification, is a priority for the Hanford Advisory Board. The cornerstone of vitrification is the Waste Treatment Plant. We all want the WTP to work safely and effectively. The Tank Waste Committee and the Health Safety and Environmental Protection Committee are bringing this advice forward today in response to

  12. Cultural Resource Conservation | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Services » Environment » Environmental Policy and Assistance » Cultural Resource Conservation Cultural Resource Conservation DOE is committed to responsible stewardship of cultural resources on its lands and has an obligation to protect these resources for future generations. Many rich and varied natural and cultural resources are present on the Departments lands and waters that contain archaeological and historical sites, threatened and endangered species, marine mammals, Native American

  13. "Berkeley Lab Cultural Festival," October 26, 2011

    ScienceCinema (OSTI)

    Lee, Soo; Simon, Horst; Grasz, Erna; Carl, Rachel; Ezeife, Loretta; Serafino, Adel;

    2013-05-29

    This is the First Annual Berkeley Lab Cultural Festival, sponsored by the Lab-Wide Diversity and Inclusion Council subcommittee.

  14. Word Pro - S2

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Table 2.7 U.S. Government Energy Consumption by Agency, Fiscal Years (Trillion Btu) Fiscal Year a Agri- culture Defense Energy GSA b HHS c Interior Justice NASA d Postal Service ...

  15. A literature review of safety culture.

    SciTech Connect (OSTI)

    Cole, Kerstan Suzanne; Stevens-Adams, Susan Marie; Wenner, Caren A.

    2013-03-01

    Workplace safety has been historically neglected by organizations in order to enhance profitability. Over the past 30 years, safety concerns and attention to safety have increased due to a series of disastrous events occurring across many different industries (e.g., Chernobyl, Upper Big-Branch Mine, Davis-Besse etc.). Many organizations have focused on promoting a healthy safety culture as a way to understand past incidents, and to prevent future disasters. There is an extensive academic literature devoted to safety culture, and the Department of Energy has also published a significant number of documents related to safety culture. The purpose of the current endeavor was to conduct a review of the safety culture literature in order to understand definitions, methodologies, models, and successful interventions for improving safety culture. After reviewing the literature, we observed four emerging themes. First, it was apparent that although safety culture is a valuable construct, it has some inherent weaknesses. For example, there is no common definition of safety culture and no standard way for assessing the construct. Second, it is apparent that researchers know how to measure particular components of safety culture, with specific focus on individual and organizational factors. Such existing methodologies can be leveraged for future assessments. Third, based on the published literature, the relationship between safety culture and performance is tenuous at best. There are few empirical studies that examine the relationship between safety culture and safety performance metrics. Further, most of these studies do not include a description of the implementation of interventions to improve safety culture, or do not measure the effect of these interventions on safety culture or performance. Fourth, safety culture is best viewed as a dynamic, multi-faceted overall system composed of individual, engineered and organizational models. By addressing all three components of

  16. Alcohol production from agricultural and forestry residues

    SciTech Connect (OSTI)

    Opilla, R.; Dale, L.; Surles, T.

    1980-05-01

    A variety of carbohydrate sources can be used as raw material for the production of ethanol. Section 1 is a review of technologies available for the production of ethanol from whole corn. Particular emphasis is placed on the environmental aspects of the process, including land utilization and possible air and water pollutants. Suggestions are made for technological changes intended to improve the economics of the process as well as to reduce some of the pollution from by-product disposal. Ethanol may be derived from renewable cellulosic substances by either enzymatic or acid hydrolysis of cellulose to sugar, followed by conventional fermentation and distillation. Section 2 is a review of the use of two agricultural residues - corn stover (field stalks remaining after harvest) and straw from wheat crops - as a cellulosic feedstock. Two processes have been evaluated with regard to environmental impact - a two-stage acid process developed by G.T. Tsao of Purdue University and an enzymatic process based on the laboratory findings of C.R. Wilke of the University of California, Berkeley. Section 3 deals with the environmental residuals expected from the manufacture of methyl and ethyl alcohols from woody biomass. The methanol is produced in a gasification process, whereas ethanol is produced by hydrolysis and fermentation processes similar to those used to derive ethanol from cellulosic materials.

  17. Alcohol production from agricultural and forestry residues

    SciTech Connect (OSTI)

    Dale, L; Opilla, R; Surles, T

    1980-09-01

    Technologies available for the production of ethanol from whole corn are reviewed. Particular emphasis is placed on the environmental aspects of the process, including land utilization and possible air and water pollutants. Suggestions are made for technological changes intended to improve the economics of the process as well as to reduce some of the pollution from by-product disposal. Ethanol may be derived from renewable cellulosic substances by either enzymatic or acid hydrolysis of cellulose to sugar, followed by conventional fermentation and distillation. The use of two agricultural residues - corn stover (field stalks remaining after harvest) and straw from wheat crops - is reviewed as a cellulosic feedstock. Two processes have been evaluated with regard to environmental impact - a two-stage acid process developed by G.T. Tsao of Purdue University and an enzymatic process based on the laboratory findings of C.R. Wilke of the University of California, Berkeley. The environmental residuals expected from the manufacture of methyl and ethyl alcohols from woody biomass are covered. The methanol is produced in a gasification process, whereas ethanol is produced by hydrolysis and fermentation processes similar to those used to derive ethanol from cellulosic materials.

  18. Algal Culture Management and Strain Selection Workshop

    Office of Energy Efficiency and Renewable Energy (EERE)

    ATP3 (Algae Testbed Public-Private Partnership) will be hosting the Microalgal Culture Management and Strain Selection Workshop August 24–28, 2015, at The University of Texas at Austin. Topics will include isolating and identifying microalgae, handling and managing microalgal cultures, screening for desirable characteristics, genetically improving strains, and analyzing lipids and higher-value products. Workshop modules will include hands-on bioprospecting, performing sample measurements, monitoring cultures for contaminants, and analyzing algal biomass composition.

  19. Montana Cultural Records Webpage | Open Energy Information

    Open Energy Info (EERE)

    LibraryAdd to library Web Site: Montana Cultural Records Webpage Abstract Provides access to the Montana Antiquities Database and provides information about the structure and...

  20. Cultural Roadmap Meeting | OpenEI Community

    Open Energy Info (EERE)

    Team met with members of the geothermal permitting community who had experience and involvement in navigating the tribal and cultural process. During the afternoon workshop,...

  1. MOWII Webinar: Wind Development Cultural Resource Management

    Broader source: Energy.gov [DOE]

    During the planning stages, wind energy development can be affected by the regulatory process relative to cultural resource management issues. Section 106 of the National Historic Preservation Act ...

  2. Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices

    DOE Patents [OSTI]

    An, Yuehuei H.; Mironov, Vladimir A.; Gutowska, Anna

    2000-01-01

    A gelling cell culture medium useful for forming a three dimensional matrix for cell culture in vitro is prepared by copolymerizing an acrylamide derivative with a hydrophilic comonomer to form a reversible (preferably thermally reversible) gelling linear random copolymer in the form of a plurality of linear chains having a plurality of molecular weights greater than or equal to a minimum gelling molecular weight cutoff, mixing the copolymer with an aqueous solvent to form a reversible gelling solution and adding a cell culture medium to the gelling solution to form the gelling cell culture medium. Cells such as chondrocytes or hepatocytes are added to the culture medium to form a seeded culture medium, and temperature of the medium is raised to gel the seeded culture medium and form a three dimensional matrix containing the cells. After propagating the cells in the matrix, the cells may be recovered by lowering the temperature to dissolve the matrix and centrifuging.

  3. Cultural intelligence support for military operations

    SciTech Connect (OSTI)

    Guthormsen, Amy M.; MacKerrow, Edward P; Merritt, Terence M; Morgart, Ruth E

    2010-04-08

    It has long been recognized that military success relies on knowledge of the enemy. In the context of standard warfare, adequate knowledge of the enemy may be gained by analyzing observable, measurable data. In the context of modern counterinsurgency operations and the global war on terror, the task of predicting the behavior of the enemy is vastly more complex and difficult. Without an understanding of the ways individuals in the host nation interpret and react to events, no amount of objective information can provide the insight required to accurately predict behavior. US military doctrine has begun to recognize the importance of the many ways that local culture can affect operation success. Increasingly military decision makers use cultural information in the service of operation planning, and troops are provided with pre-deployment cultural training. However, no amount of training can cover the breadth and depth of potentially useful cultural information, and no amount of careful planning can avoid the need to adapt as situations develop. Therefore, a critical challenge is to provide useful tools to US personnel in their efforts to collect, analyze, and utilize cultural information. Essential functions for cultural support tools include the following: (1) to narrow down a broad range of available data and focus the user's attention on context-relevant information, (2) to present cultural information in an easily understood form, (3) to prompt the user to seek relevant information in the environment, (4) to synthesize information, and (5) to predict outcomes based on possible courses of operation. In this paper, we begin by reviewing the ways in which military operations can benefit from cultural intelligence. We then discuss frameworks for analyzing cultural information in the context of a military operation. We conclude with a demonstration of our current efforts to develop a tool that meets the aforementioned functional challenges.

  4. Sector-specific issues and reporting methodologies supporting the General Guidelines for the voluntary reporting of greenhouse gases under Section 1605(b) of the Energy Policy Act of 1992. Volume 2: Part 4, Transportation sector; Part 5, Forestry sector; Part 6, Agricultural sector

    SciTech Connect (OSTI)

    Not Available

    1994-10-01

    This volume, the second of two such volumes, contains sector-specific guidance in support of the General Guidelines for the voluntary reporting of greenhouse gas emissions and carbon sequestration. This voluntary reporting program was authorized by Congress in Section 1605(b) of the Energy Policy Act of 1992. The General Guidelines, bound separately from this volume, provide the overall rationale for the program, discuss in general how to analyze emissions and emission reduction/carbon sequestration projects, and address programmatic issues such as minimum reporting requirements, time parameters, international projects, confidentiality, and certification. Together, the General Guidelines and the guidance in these supporting documents will provide concepts and approaches needed to prepare the reporting forms. This second volume of sector-specific guidance covers the transportation sector, the forestry sector, and the agricultural sector.

  5. Department of Energy Management of Cultural Resources

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2001-05-02

    The purpose of this Policy is to ensure that Department of Energy (DOE) programs, including the National Nuclear Security Administration (NNSA), and field elements integrate cultural resources management into their missions and activities. Certified 1-28-11. No cancellation.

  6. Cultural Artifacts Cross Eras at the NNSS

    Broader source: Energy.gov [DOE]

    It is well known that the Nevada National Security Site (NNSS) is home to many artifacts from the Cold War. But few people may be aware of the older important cultural resources that exist throughout the site.

  7. Integrated Safety Management Safety Culture Resources | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Safety Culture Resources Integrated Safety Management Safety Culture Resources A collection of resources available in implementing ISM safety culture activities Safety from the Operator's Perspective: We are All in This Together (2005) Transcript, Keeping the Edge: Enhancing Performance Through Managing Culture (2003), Edgar H. Schein, Ph.D. Proceedings of the Advisory Committee on Reactor Safeguards Safety Culture Workshop (2003) Safety Culture in Nuclear Installations: Guidance for

  8. Idaho National Laboratory Cultural Resource Management Plan

    SciTech Connect (OSTI)

    Lowrey, Diana Lee

    2009-02-01

    As a federal agency, the U.S. Department of Energy has been directed by Congress, the U.S. president, and the American public to provide leadership in the preservation of prehistoric, historic, and other cultural resources on the lands it administers. This mandate to preserve cultural resources in a spirit of stewardship for the future is outlined in various federal preservation laws, regulations, and guidelines such as the National Historic Preservation Act, the Archaeological Resources Protection Act, and the National Environmental Policy Act. The purpose of this Cultural Resource Management Plan is to describe how the Department of Energy, Idaho Operations Office will meet these responsibilities at the Idaho National Laboratory. This Laboratory, which is located in southeastern Idaho, is home to a wide variety of important cultural resources representing at least 13,500 years of human occupation in the southeastern Idaho area. These resources are nonrenewable; bear valuable physical and intangible legacies; and yield important information about the past, present, and perhaps the future. There are special challenges associated with balancing the preservation of these sites with the management and ongoing operation of an active scientific laboratory. The Department of Energy, Idaho Operations Office is committed to a cultural resource management program that accepts these challenges in a manner reflecting both the spirit and intent of the legislative mandates. This document is designed for multiple uses and is intended to be flexible and responsive to future changes in law or mission. Document flexibility and responsiveness will be assured through annual reviews and as-needed updates. Document content includes summaries of Laboratory cultural resource philosophy and overall Department of Energy policy; brief contextual overviews of Laboratory missions, environment, and cultural history; and an overview of cultural resource management practices. A series of

  9. Idaho National Laboratory Cultural Resource Management Plan

    SciTech Connect (OSTI)

    Lowrey, Diana Lee

    2011-02-01

    As a federal agency, the U.S. Department of Energy has been directed by Congress, the U.S. president, and the American public to provide leadership in the preservation of prehistoric, historic, and other cultural resources on the lands it administers. This mandate to preserve cultural resources in a spirit of stewardship for the future is outlined in various federal preservation laws, regulations, and guidelines such as the National Historic Preservation Act, the Archaeological Resources Protection Act, and the National Environmental Policy Act. The purpose of this Cultural Resource Management Plan is to describe how the Department of Energy, Idaho Operations Office will meet these responsibilities at the Idaho National Laboratory. This Laboratory, which is located in southeastern Idaho, is home to a wide variety of important cultural resources representing at least 13,500 years of human occupation in the southeastern Idaho area. These resources are nonrenewable; bear valuable physical and intangible legacies; and yield important information about the past, present, and perhaps the future. There are special challenges associated with balancing the preservation of these sites with the management and ongoing operation of an active scientific laboratory. The Department of Energy, Idaho Operations Office is committed to a cultural resource management program that accepts these challenges in a manner reflecting both the spirit and intent of the legislative mandates. This document is designed for multiple uses and is intended to be flexible and responsive to future changes in law or mission. Document flexibility and responsiveness will be assured through annual reviews and as-needed updates. Document content includes summaries of Laboratory cultural resource philosophy and overall Department of Energy policy; brief contextual overviews of Laboratory missions, environment, and cultural history; and an overview of cultural resource management practices. A series of

  10. Idaho National Laboratory Cultural Resource Management Plan

    SciTech Connect (OSTI)

    Julie Braun Williams

    2013-02-01

    As a federal agency, the U.S. Department of Energy has been directed by Congress, the U.S. president, and the American public to provide leadership in the preservation of prehistoric, historic, and other cultural resources on the lands it administers. This mandate to preserve cultural resources in a spirit of stewardship for the future is outlined in various federal preservation laws, regulations, and guidelines such as the National Historic Preservation Act, the Archaeological Resources Protection Act, and the National Environmental Policy Act. The purpose of this Cultural Resource Management Plan is to describe how the Department of Energy, Idaho Operations Office will meet these responsibilities at Idaho National Laboratory in southeastern Idaho. The Idaho National Laboratory is home to a wide variety of important cultural resources representing at least 13,500 years of human occupation in the southeastern Idaho area. These resources are nonrenewable, bear valuable physical and intangible legacies, and yield important information about the past, present, and perhaps the future. There are special challenges associated with balancing the preservation of these sites with the management and ongoing operation of an active scientific laboratory. The Department of Energy, Idaho Operations Office is committed to a cultural resource management program that accepts these challenges in a manner reflecting both the spirit and intent of the legislative mandates. This document is designed for multiple uses and is intended to be flexible and responsive to future changes in law or mission. Document flexibility and responsiveness will be assured through regular reviews and as-needed updates. Document content includes summaries of Laboratory cultural resource philosophy and overall Department of Energy policy; brief contextual overviews of Laboratory missions, environment, and cultural history; and an overview of cultural resource management practices. A series of appendices

  11. Integration of Safety Culture Attributes into EFCOG Work Planning...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Planning and Control Guidance Document Integration of Safety Culture Attributes into EFCOG ... Topics Covered: Integration of Safety Culture (SC) Attributes into EFCOG Work Planning and ...

  12. Assessment of Nuclear Safety Culture at the Salt Waste Processing...

    Office of Environmental Management (EM)

    Oversight Assessment of Nuclear Safety Culture at the Salt Waste Processing Facility ... of Nuclear Safety Culture at the Salt Waste Processing Facility Project Table of ...

  13. Independent Oversight Follow-up Assessment of Safety Culture...

    Office of Environmental Management (EM)

    Independent Oversight Follow-up Assessment of Safety Culture at the Waste Treatment and ... Assessment of Safety Culture at the Waste Treatment and Immobilization Plant Table ...

  14. Wyoming Department of State Parks and Cultural Resources | Open...

    Open Energy Info (EERE)

    Cultural Resources Jump to: navigation, search Name: Wyoming Department of State Parks and Cultural Resources Abbreviation: SPCR Address: 2301 Central Avenue Place: Cheyenne,...

  15. Self-Reliability and Motivation in a Nuclear Security Culture...

    Office of Scientific and Technical Information (OSTI)

    and Motivation in a Nuclear Security Culture Enhancement Program Citation Details In-Document Search Title: Self-Reliability and Motivation in a Nuclear Security Culture ...

  16. BLM Manual 8140 - Protecting Cultural Resources | Open Energy...

    Open Energy Info (EERE)

    section provides general guidance for protecting cultural resources from natural or human-caused deterioration; for making decisions about recovering significant cultural...

  17. Independent Oversight Follow-up Assessment of Safety Culture...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Assessment of Safety Culture at the Waste Treatment and Immobilization Plant - June 2014 Independent Oversight Follow-up Assessment of Safety Culture at the Waste Treatment and...

  18. Enterprise Assessments Follow-up Assessment of Safety Culture...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Assessment of Safety Culture at the Hanford Site Waste Treatment and Immobilization Plant - June 2015 Enterprise Assessments Follow-up Assessment of Safety Culture at the Hanford...

  19. Build Replication into Corporate Culture | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Build Replication into Corporate Culture Build Replication into Corporate Culture This presentation addresses how to build replication into the corporate culture. Build Replication into Corporate Culture (August 23, 2011) (1.87 MB) More Documents & Publications Implementing a Corporate Energy Management System 3M's Model Rewards and Recognition Program Engages Employees and Drives Energy Savings Efforts Replicate Best Practices

  20. Cultural Resource Program and Curation Services - Hanford Site

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Public Safety and Resource Protection (PSRP) Cultural Resource Program and Curation Services Public Safety and Resource Protection (PSRP) Public Safety and Resource Protection Home Cultural Resource Program and Curation Services Ecological Monitoring Environmental Surveillance Meteorology and Climatology Services Seismic Monitoring Cultural Resource Program and Curation Services Email Email Page | Print Print Page | Text Increase Font Size Decrease Font Size Cultural Resource Program and

  1. Increasing cell culture population doublings for long-term growth of finite life span human cell cultures

    DOE Patents [OSTI]

    Stampfer, Martha R; Garbe, James C

    2015-02-24

    Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.

  2. Corporate Culture | National Nuclear Security Administration | (NNSA)

    National Nuclear Security Administration (NNSA)

    Corporate Culture NNSA's annual budget is more than $10 billion. Our employees use their scientific, technical and professional expertise to oversee world-class science and engineering programs that use the world's largest and fastest computers, along with other state-of-the-art equipment that push the envelope of creativity and innovation. NNSA's annual budget is more than $10 billion. Our employees use their scientific, technical and professional expertise to oversee world-class science and

  3. Hanford Site, Tribes Raise Awareness of Culturally Significant...

    Office of Environmental Management (EM)

    volcanic glass, which is part of a simulated fire hearth at the cultural test beds site. ... volcanic glass, which is part of a simulated fire hearth at the cultural test beds site. ...

  4. Track 1: Safety Culture- Taking ISMS to the Next Level

    Broader source: Energy.gov [DOE]

    ISM Workshop Presentations Knoxville Convention Center, Knoxville, TN August 2009 Track 1: Safety Culture - Taking ISMS to the Next Level

  5. Making Energy Efficiency Part of Corporate Culture | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Making Energy Efficiency Part of Corporate Culture Making Energy Efficiency Part of Corporate Culture This presentation discusses how Nissan Motor Company incorporated energy efficiency into its corporate environment through executive management support and cross-functional corporate teams. Making Energy Efficiency Part of Corporate Culture (June 12, 2012) (6.61 MB) More Documents & Publications Energy Management and Financing Nissan Showcases the Results of an Energy-Wise Corporate Culture

  6. Sponsoring, Mentoring and Guiding Cultural Diverse Young Scientists...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Sponsoring, Mentoring and Guiding Cultural Diverse Young Scientists and Engineers Presentation on successful mentoring. PDF icon Partnership meeting presentation 020711...

  7. Hanford Advisory Board Advice: Testing Safety Culture in Practice,

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hanford Advisory Board Advice: Testing Safety Culture in Practice, Draft 3, Feb 3, 2016 Lead Issue Managers: Dirk Dunning and Liz Mattson Background The Hanford Advisory Board (HAB, Board) has been focused on reviewing, discussing, and issuing advice on safety culture since the Defense Nuclear Facilities Safety Board issued its Recommendation 2011-1: Safety Culture at the Waste Treatment and Immobilization Plant in June 2011. Safety culture terminology has created confusion in our discussions,

  8. Welcome to the Cultural Resources Newsletter | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Welcome to the Cultural Resources Newsletter Welcome to the Cultural Resources Newsletter Welcome to the Cultural Resources Newsletter (446.29 KB) More Documents & Publications Secretary Directs FPO to Prepare Strategic Plan Strategic Plan Submitted to the Secretary Microsoft Word - August06.doc

  9. Safety Culture at the WTP White Paper: Potential Attachment for Advice on Waste Treatment Plant Safety Culture

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    29/2011 Page 1 of 6 Safety Culture at the WTP White Paper: Potential Attachment for Advice on Waste Treatment Plant Safety Culture Introduction This white paper provides context for the Hanford Advisory Board's (HAB) concerns regarding safety culture at the Waste Treatment Plant (WTP). This document is intended to clarify terminology associated with "safety culture" and to provide background about its conception, application, and development. The HAB has advised that a rigorous safety

  10. Training Framework to Improve the DOE Performance-Based Culture |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Training Framework to Improve the DOE Performance-Based Culture Training Framework to Improve the DOE Performance-Based Culture Guidance Memorandum for implementing the Secretaries initiative for improving the Performanced-Based Culture of the Department. Training Framework Improve Performance (239.56 KB) Responsible Contacts N. Tony Nguyen PROGRAM ANALYST E-mail tony.nguyen@hq.doe.gov Phone 202-586-4533 More Documents & Publications Audit Report: IG-0510 Annual

  11. DOE - NNSA/NFO -- Photo Library Cultural Resources

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cultural Resources NNSA/NFO Language Options U.S. DOE/NNSA - Nevada Field Office Photo Library - Cultural Resources Prehistoric cultural resources are abundant at the Nevada National Security Site, indicating Native Americans have occupied the region for more than 10,000 years. Historic artifacts of more recent origin are also present, reflecting use by miners, ranchers and settlers who traveled through the area. Instructions: Click the photograph THUMBNAIL to view the photograph details Click

  12. Integration of Safety Culture Attributes into EFCOG Work Planning and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Control Guidance Document | Department of Energy Safety Culture Attributes into EFCOG Work Planning and Control Guidance Document Integration of Safety Culture Attributes into EFCOG Work Planning and Control Guidance Document May 15, 2013 Presenters: Steele Coddington, NSTec, Las Vegas, and John McDonald, WRPS, Hanford Topics Covered: Integration of Safety Culture (SC) Attributes into EFCOG Work Planning and Control Guidance Document Linking SC to WP&C CRADS EFCOG and DOE SC and WP&C

  13. Indoctrinating Subcontractors into the DOE Safety Culture and...

    Office of Environmental Management (EM)

    Many of WCH subcontracts target small businesses with limited experience in the DOE safety culture. Safety Performance is factored into the incentive fee structure of the RCCC ...

  14. Wyoming Department of State Parks and Cultural Resources and...

    Open Energy Info (EERE)

    and Historic Sites - Rules and Regulations, Chapter 1Legal Abstract This chapter sets forth the rules and regulations of the Wyoming Department of State Parks and Cultural...

  15. CSB Investigations and Safety Culture | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    from occurring again. Analysis of Safety Systems CSB Investigations and Safety Culture More Documents & Publications Nuclear Safety Workshop Summary Operating Experience...

  16. BLM Manual 8120 - Tribal Consultation Under Cultural Resources...

    Open Energy Info (EERE)

    search OpenEI Reference LibraryAdd to library PermittingRegulatory Guidance - GuideHandbook: BLM Manual 8120 - Tribal Consultation Under Cultural ResourcesPermitting...

  17. BLM Manual 8110 - Identifying and Evaluating Cultural Resources...

    Open Energy Info (EERE)

    search OpenEI Reference LibraryAdd to library PermittingRegulatory Guidance - GuideHandbook: BLM Manual 8110 - Identifying and Evaluating Cultural ResourcesPermitting...

  18. Key Practical Issues in Strengthening Safety Culture, INSAG-15

    Office of Energy Efficiency and Renewable Energy (EERE)

    Key Pratical Issues in Strengthening Safety Culture, INSAG-15. A report by the International Nuclear Safety Advisory Gorup, International Atomic Energy Agency, Vienna, 2002.

  19. Self-Reliability and Motivation in a Nuclear Security Culture...

    Office of Scientific and Technical Information (OSTI)

    Conference: Self-Reliability and Motivation in a Nuclear Security Culture Enhancement Program Citation Details In-Document Search Title: Self-Reliability and Motivation in a ...

  20. Pueblo De San Ildefonso Department of Environmental and Cultural...

    Energy Savers [EERE]

    Environmental and Cultural Preservation Program Overview 2015 Topic: Director Raymond Martinez, and Governor Mountain, provided the membership with an update on the Pueblo de San...

  1. "Violent Intent Modeling: Incorporating Cultural Knowledge into the Analytical Process

    SciTech Connect (OSTI)

    Sanfilippo, Antonio P.; Nibbs, Faith G.

    2007-08-24

    While culture has a significant effect on the appropriate interpretation of textual data, the incorporation of cultural considerations into data transformations has not been systematic. Recognizing that the successful prevention of terrorist activities could hinge on the knowledge of the subcultures, Anthropologist and DHS intern Faith Nibbs has been addressing the need to incorporate cultural knowledge into the analytical process. In this Brown Bag she will present how cultural ideology is being used to understand how the rhetoric of group leaders influences the likelihood of their constituents to engage in violent or radicalized behavior, and how violent intent modeling can benefit from understanding that process.

  2. Essential Innovations Ekistics Town Planning Jiangsu Sifang Culture...

    Open Energy Info (EERE)

    Geothermal energy Product: Announced in October 2006, this is a JV between Essential Innovation of Canada, Ekistics Town Planning also of Canada, and Jiangsu Sifang Culture...

  3. Sponsoring, Mentoring and Guiding Cultural Diverse Young Scientists and

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Engineers | Argonne National Laboratory Sponsoring, Mentoring and Guiding Cultural Diverse Young Scientists and Engineers Presentation on successful mentoring. PDF icon Partnership meeting presentation 020711

  4. Independent Oversight Assessment of the Nuclear Safety Culture...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Health, Safety and Security HSS Independent Oversight Assessment of Nuclear Safety Culture and Management of Nuclear Safety ... EM Office of Environmental Management EM-1 ...

  5. A GIS approach to cultural resources management and NEPA compliance

    SciTech Connect (OSTI)

    Moeller, K.

    1996-06-01

    Cultural resources management and historic preservation compliance are best approached within the broader framework of natural resources planning and land management. Argonne National Laboratory is currently assisting federal agencies with the development of computer- based resource management systems for large facilities, and cultural resources management and preservation are components of these systems. In the area of cultural resources, Argonne is using the GIS tool to demonstrate how federal facilities can manage large, complex databases, integrate cultural resource data with other environmental variables, model distributions of resources to aid in inventory and evaluation, link the data to quantitative and impact modes, and effectively manage and monitor resource planning activities and environmental compliance.

  6. Fluorescent tracking of nickel ions in human cultured cells ...

    Office of Scientific and Technical Information (OSTI)

    Fluorescent tracking of nickel ions in human cultured cells Citation Details In-Document ... B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International ...

  7. Characterization of anaerobic microbial culture with high acidogenic activity

    SciTech Connect (OSTI)

    De la Torre, I.; Goma, G.

    1981-01-01

    The mixed cultures which were used were isolated from municipal sludge digestors, and the production of organic acids (acetic, propionic, butyric, etc.) from carbohydrates was tested. The behavior of the reference population (culture R) obtained directly from the sewage treatment plant, is compared to that obtained after three months in a plug-flow reactor (Gradostat fermentor) without pH control (culture A) and after six months with pH control (culture B). For culture B, the specific rate of acid production is related to the cell growth rate by (1/X)rp equals 17 mu plus 1.6 with a 0.36 g/g for the initial culture (R) to 0.72 g/g for culture B after six months in continuous culture, and 0.8 g/g in plug-flow continuous culture. The productivity of organic acids reaches 1.7 g/liter/hour. It is suggested that the acidogenic fermentation, the first step of methanogenesis, is a potential process to produce acetic, propionic, and butyric acids.

  8. DOE Policy 141.1: Management of Cultural Resources

    Broader source: Energy.gov [DOE]

    The purpose of DOE Policy 141.1 is to ensure that Department of Energy (DOE) programs and field elements integrate cultural resources management into their missions and activities and to raise the level of awareness and accountability among DOE contractors concerning the importance of the Department’s cultural resource-related legal and trust responsibilities.

  9. Preservation technologies; tools for enhanced cultural resource management

    SciTech Connect (OSTI)

    Culbertson, J.D.

    1995-12-01

    Legislation enacted since the mid sixties has defined requirements for cultural resource management. This is an important area of environmental management that has received only limited attention. Cultural resources are integral to environmental systems; they need to be considered in any resource management activities. They also provide important information about long term changes in environmental systems and the effects of human activity.

  10. Idaho National Laboratory Cultural Resource Monitoring Report for FY 2009

    SciTech Connect (OSTI)

    Brenda R. Pace; Julie B. Braun

    2009-10-01

    This report describes the cultural resource monitoring activities of the Idaho National Laboratory’s (INL) Cultural Resource Management (CRM) Office during fiscal year 2009 (FY 2009). Throughout the year, thirty-eight cultural resource localities were revisited including: two locations with Native American human remains, one of which is a cave, two additional caves, twenty-two prehistoric archaeological sites, six historic homesteads, two historic stage stations, two historic trails, and two nuclear resources, including Experimental Breeder Reactor-I, which is a designated National Historic Landmark. Several INL project areas were also monitored in FY 2009 to assess project compliance with cultural resource recommendations and monitor the effects of ongoing project activities. Although impacts were documented at a few locations and trespassing citations were issued in one instance, no significant adverse effects that would threaten the National Register eligibility of any resources were observed. Monitoring also demonstrated that several INL projects generally remain in compliance with recommendations to protect cultural resources.

  11. Reducing Forestry Emissions in Indonesia | Open Energy Information

    Open Energy Info (EERE)

    critically at the trade-offs between development pathways based on land-intensive enterprises and climate change mitigation. Without a coordinated approach to multiple...

  12. Third world applications of pyrolysis of agricultural and forestry wastes

    SciTech Connect (OSTI)

    Tatom, J.W.; Wellborn, H.W.; Harahap, F.; Sasmojo, S.

    1980-01-01

    The development of an appropriate technology for the conversion of agricultural and wood wastes into fuels in underdeveloped nations is discussed. Low temperature pyrolysis offers a promising means of conversion since the char and oil products are storable and easily transportable. The steady-flow, vertical packed bed, partial oxidation pyrolysis process is described and the appropriate technology pyrolytic converter basic design concept is presented. The current status of program in the US and in Papua New Guinea is described. The operation, test results, and economics of the converter are discussed.

  13. Forestry management for sustainable development. EDI Policy Seminar Report 32

    SciTech Connect (OSTI)

    D`Silva, E.; Appanah, S.

    1993-09-01

    Forests will continue to disappear rapidly, the authors contend, until they are recognized as a valuable economic resource. This paper examines the causes of deforestation in Asia and suggests practical ways to achieve sustainable forest management. The report focuses on commercial logging, demand for firewood and fodder, and clearing forest land for farming. Economic policies and forest institutions have failed to protect natural forests. The authors point out technical problems that hinder forest management, such as improper tree harvesting. They describe conflicting goals among forest users and government investments that deplete forests. The authors argue that sustainable forest management calls for sound pricing policies and strong institutions to enforce them. They discuss benefit-sharing schemes that give local people incentives to protect forests and new ways to manage tree plantations to serve many different users. Detailed case studies look at effective forest management programs in Indonesia, Malaysia, Papua New Guinea, and the Philippines. The paper examines profitable opportunities for trade in environmental services. Such trade would involve industrial countries paying developing nations not to clear their natural forests. The protected forests would help reduce global carbon emissions and preserve biodiversity.

  14. FINAL TECHNICAL REPORT FOR FORESTRY BIOFUEL STATEWIDE COLLABORATION CENTER (MICHIGAN)

    SciTech Connect (OSTI)

    LaCourt, Donna M.; Miller, Raymond O.; Shonnard, David R.

    2012-04-24

    A team composed of scientists from Michigan State University (MSU) and Michigan Technological University (MTU) assembled to better understand, document, and improve systems for using forest-based biomass feedstocks in the production of energy products within Michigan. Work was funded by a grant (DE-EE-0000280) from the U.S. Department of Energy (DOE) and was administered by the Michigan Economic Development Corporation (MEDC). The goal of the project was to improve the forest feedstock supply infrastructure to sustainably provide woody biomass for biofuel production in Michigan over the long-term. Work was divided into four broad areas with associated objectives: • TASK A: Develop a Forest-Based Biomass Assessment for Michigan – Define forest-based feedstock inventory, availability, and the potential of forest-based feedstock to support state and federal renewable energy goals while maintaining current uses. • TASK B: Improve Harvesting, Processing and Transportation Systems – Identify and develop cost, energy, and carbon efficient harvesting, processing and transportation systems. • TASK C: Improve Forest Feedstock Productivity and Sustainability – Identify and develop sustainable feedstock production systems through the establishment and monitoring of a statewide network of field trials in forests and energy plantations. • TASK D: Engage Stakeholders – Increase understanding of forest biomass production systems for biofuels by a broad range of stakeholders. The goal and objectives of this research and development project were fulfilled with key model deliverables including: 1) The Forest Biomass Inventory System (Sub-task A1) of feedstock inventory and availability and, 2) The Supply Chain Model (Sub-task B2). Both models are vital to Michigan’s forest biomass industry and support forecasting delivered cost, as well as carbon and energy balance. All of these elements are important to facilitate investor, operational and policy decisions. All other sub-tasks supported the development of these two tools either directly or by building out supporting information in the forest biomass supply chain. Outreach efforts have, and are continuing to get these user friendly models and information to decision makers to support biomass feedstock supply chain decisions across the areas of biomass inventory and availability, procurement, harvest, forwarding, transportation and processing. Outreach will continue on the project website at http://www.michiganforestbiofuels.org/ and http://www.michiganwoodbiofuels.org/

  15. Agriculture, Forestry and Other Land Use Mitigation Project Database...

    Open Energy Info (EERE)

    countries. The database currently holds 497 project entries from 11 different databases, including formal crediting scheme registries and third party compilations."...

  16. Regional Community Forestry Training Center for Asia and the...

    Open Energy Info (EERE)

    is that strong capacities are crucial for successfully engaging local people in forest management. This concept forms the basis of RECOFTC's strategic and program plans for the...

  17. BioenergizeME Virtual Science Fair: Bioenegy Benefits Environmental Forestry

    Broader source: Energy.gov [DOE]

    This infographic was created by students from Sun Valley High School in Aston, PA, as part of the U.S. Department of Energy-BioenergizeME Virtual Science Fair.

  18. Forestry and Poverty Data in Vietnam: Status, Gaps, and Potential...

    Open Energy Info (EERE)

    Type: Dataset, Publications Website: recoftc.orgsitefileadmindocspublicationsTheGreyZone2009Forest Country: Vietnam UN Region: South-Eastern Asia Coordinates:...

  19. Forestry mitigation potential and costs in developing countries - Preface

    SciTech Connect (OSTI)

    Sathaye, Jayant A.; Makundi, Willy; Andrasko, Kenneth

    2001-01-01

    The forest sector in Tanzania offers ample opportunities to reduce greenhouse gas emissions (GHG) and sequestered carbon (C) in terrestrial ecosystems. More than 90% of the country's demand for primary energy is obtained from biomass mostly procured unsustainably from natural forests. This study examines the potential to sequester C through expansion of forest plantations aimed at reducing the dependence on natural forest for wood fuel production, as well as increase the country's output of industrial wood from plantations. These were compared ton conservation options in the tropical and miombo ecosystems. Three sequestration options were analyzed, involving the establishment of short rotation and long rotation plantations on about 1.7 x 106 hectares. The short rotation community forest option has a potential to sequester an equilibrium amount of 197.4 x 106 Mg C by 2024 at a net benefit of $79.5 x 106, while yielding a NPV of $0.46 Mg-1 C. The long rotation options for softwood and hardwood plantations will reach an equilibrium sequestration of 5.6 and 11.8 x 106 Mg C at a negative NPV of $0.60 Mg-1 C and $0.32 Mg-1 C. The three options provide cost competitive opportunities for sequestering about 7.5 x 106 Mg C yr -1 while providing desired forest products and easing the pressure on the natural forests in Tanzania. The endowment costs of the sequestration options were all found to be cheaper than the emission avoidance cost for conservation options which had an average cost of $1.27 Mg-1 C, rising to $7.5 Mg-1 C under some assumptions on vulnerability to encroachment. The estimates shown here may represent the upper bound, because the actual potential will be influenced by market prices for inputs and forest products, land use policy constraints and the structure of global C transactions.

  20. Idaho National Laboratory Cultural Resource Monitoring Report for FY 2008

    SciTech Connect (OSTI)

    Brenda R. Pace

    2009-01-01

    This report describes the cultural resource monitoring activities of the Idaho National Laboratory’s (INL) Cultural Resource Management (CRM) Office during fiscal year 2008 (FY 2008). Throughout the year, 45 cultural resource localities were revisited including: two locations of heightened Shoshone-Bannock tribal sensitivity, four caves, one butte, twenty-eight prehistoric archaeological sites, three historic homesteads, two historic stage stations, one historic canal construction camp, three historic trails, and Experimental Breeder Reactor-I, which is a designated National Historic Landmark. Several INL project areas were also monitored in FY 2008 to assess project compliance with cultural resource recommendations, confirm the locations of previously recorded cultural resources in relation to project activities, to assess the damage caused by fire-fighting efforts, and to watch for cultural materials during ground disturbing activities. Although impacts were documented at a few locations, no significant adverse effects that would threaten the National Register eligibility of any resource were observed. Monitoring also demonstrated that INL projects generally remain in compliance with recommendations to protect cultural resources

  1. Building a Strong Culture That Produces Sustainable Performance - 13444

    SciTech Connect (OSTI)

    McDonald, John A. Jr

    2013-07-01

    Washington River Protection Solutions LLC (WRPS) has been involved with culture improvement for a number of years which has included co-chairing the industry effort to develop the EFCOG safety culture guidance documents [1, 2], and integration of this guidance into organizational processes and behavior expectations, described in more detail below. As various organizational cultural assessments have been periodically performed, and subsequent actions implemented to address improvement opportunities, organizational performance has shown improvement. Culture improvement is evident in the company's industrial safety statistics, event rates, safety culture survey results, employee morale, productivity, leadership effectiveness, and employee engagement. There does appear to be a relationship between striving to demonstrate behaviors consistent with excellent safety culture and good organizational performance over the past couple of years at WRPS. As performance continues to be evaluated, an improvement opportunity was identified to further enhance performance through field oriented behavioral/cultural improvement activities. WRPS recently conducted a three month effort to improve consistent implementation of management expectations by increasing management field presence with a focus on interacting real-time with workers and first line supervisors, and changing behaviors as appropriate. (authors)

  2. Testing the cultural theory of risk in France

    SciTech Connect (OSTI)

    Brenot, J.; Bonnefous, S.; Marris, C.

    1998-12-01

    Cultural Theory, as developed by Mary Douglas, argues that differing risk perceptions can be explained by reference to four distinct cultural biases: hierarchy, egalitarianism, individualism, and fatalism. This paper presents empirical results from a quantitative survey based on a questionnaire devised by Karl Dake to measure these cultural biases. A large representative sample was used to test this instrument in the French social context. Correlations between cultural biases and perceptions of 20 social and environmental risks were examined. These correlations were very weak, but were statistically significant: cultural biases explained 6%, at most, of the variance in risk perceptions. Standard socio-demographic variables were also weakly related to risk perceptions (especially gender, social class, and education), and cultural biases and socio-demographic variables were themselves intercorrelated (especially with age, social class, and political outlook). The authors compare these results with surveys conducted in other countries using the same instrument and conclude that new methods, more qualitative and contextual, still need to be developed to investigate the cultural dimensions of risk perceptions. The paper also discusses relationships between perceptions of personal and residual risk, and between perceived risk and demand for additional safety measures. These three dimensions were generally closely related, but interesting differences were observed for some risk issues. Included in the list of risk perceptions were pollution, hazardous materials, and radioactive wastes.

  3. Idaho National Laboratory Cultural Resource Monitoring Report for 2013

    SciTech Connect (OSTI)

    Julie B. Williams; Brenda Pace

    2013-10-01

    This report describes the cultural resource monitoring activities of the Idaho National Laboratory’s (INL) Cultural Resource Management (CRM) Office during 2013. Throughout the year, thirty-eight cultural resource localities were revisited including: two locations with Native American human remains, one of which is also a cave; fourteen additional caves; seven prehistoric archaeological sites ; four historic archaeological sites; one historic trail; one nuclear resource (Experimental Breeder Reactor-I, a designated National Historic Landmark); and nine historic structures located at the Central Facilities Area. Of the monitored resources, thirty-three were routinely monitored, and five were monitored to assess project compliance with cultural resource recommendations along with the effects of ongoing project activities. On six occasions, ground disturbing activities within the boundaries of the Power Burst Facility/Critical Infrastructure Test Range Complex (PBF/CITRC) were observed by INL CRM staff prepared to respond to any additional finds of Native American human remains. In addition, two resources were visited more than once as part of the routine monitoring schedule or to monitor for additional damage. Throughout the year, most of the cultural resources monitored had no visual adverse changes resulting in Type 1determinations. However, Type 2 impacts were noted at eight sites, indicating that although impacts were noted or that a project was operating outside of culturally cleared limitations, cultural resources retained integrity and noted impacts did not threaten National Register eligibility. No new Type 3 or any Type 4 impacts that adversely impacted cultural resources and threatened National Register eligibility were observed at cultural resources monitored in 2013.

  4. Operational Pause at Savannah River Site Benefits Safety Culture, Operations

    Broader source: Energy.gov [DOE]

    AIKEN, S.C. – EM and the Savannah River Site (SRS) management and operations contractor are seeing positive impacts on safety culture as the site works to restore operations following last year’s operational pause.

  5. Developing safety culture-rocket science or common sense?

    SciTech Connect (OSTI)

    Mahn, J.A.

    1998-08-01

    Despite evidence of significant management contributions to the causes of major accidents, recent events at Millstone Nuclear Power Station in the US and Ontario Hydro in Canada might lead one to conclude that the significance of safety culture, and the role of management in developing and maintaining an appropriate safety culture, is either not being understood or not being taken serious as integral to the safe operation of some complex, high-reliability operations. It is the purpose of this paper to address four aspects of management that are particularly important to safety culture, and to illustrate how development of an appropriate safety culture is more a matter of common sense than rocket science.

  6. Best Seller Cites Westinghouse Safety Culture at WIPP as "World...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Westinghouse TRU Solutions LLC. "We are proud of our safety culture, and we continuously work to enhance it." Westinghouse is the management and operating contractor for the U.S....

  7. AN OVERVIEW OF CULTURAL RESOURCES ON PAHUTE AND RAINIER MESAS...

    National Nuclear Security Administration (NNSA)

    AN OVERVIEW OF CULTURAL RESOURCES ON PAHUTE AND RAINIER MESAS ON THE NEVADA TEST SITE, NYE ... Officer. Mr. Robert Bivona, Nevada Test Site Support Office, and his predecessor Mr. ...

  8. Idaho National Laboratory Cultural Resource Monitoring Report for FY 2010

    SciTech Connect (OSTI)

    INL Cultural Resource Management Office

    2010-10-01

    This report describes the cultural resource monitoring activities of the Idaho National Laboratory’s (INL) Cultural Resource Management (CRM) Office during fiscal year 2010 (FY 2010). Throughout the year, thirty-three cultural resource localities were revisited, including somethat were visited more than once, including: two locations with Native American human remains, one of which is a cave, two additional caves, twenty-six prehistoric archaeological sites, two historic stage stations, and Experimental Breeder Reactor-I, which is a designated National Historic Landmark. The resources that were monitored included seventeen that are routinely visited and sixteen that are located in INL project areas. Although impacts were documented at a few locations and one trespassing incident (albeit sans formal charges) was discovered, no significant adverse effects that would threaten the National Register eligibility of any resources were observed. Monitoring also demonstrated that several INL projects generally remain in compliance with recommendations to protect cultural resources.

  9. Best Practices Workshop for Safety Culture | Y-12 National Security...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    from 20 organizations recently met at Y-12 to share safety culture best practices and lessons learned. The two-day workshop featured more than two dozen presentations on...

  10. New culturing tool reveals a full genome from single cells

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New culturing tool reveals New culturing tool reveals a full genome from single cells A new technique for genetic analysis, "gel microdroplets," helps scientists generate complete genomes from a single cell. March 15, 2013 Two GMD containing gut-community microcolonies are shown, with green fluorescence marking the DNA. Two GMD containing gut-community microcolonies are shown, with green fluorescence marking the DNA. Photo credit A. Dichosa, Los Alamos National Laboratory. Contact

  11. Creating a Culture of Risk Assessment | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Creating a Culture of Risk Assessment Creating a Culture of Risk Assessment Decisions regarding how to secure and invest in the Nation's energy infrastructure are often complex. Limited resources and investment returns, tight budgets, and lack of information can hinder the process of how to best maintain or improve existing infrastructure or build new energy facilities and systems. Threats or hazards that can impact energy infrastructure and the consequences of those impacts must be known to

  12. Annual training event instrumental in region's safety culture |

    Energy Savers [EERE]

    Department of Energy Annual training event instrumental in region's safety culture Annual training event instrumental in region's safety culture September 8, 2014 - 10:00am Addthis Participants receive hands-on demonstrations for protective equipment. This year’s event offers 45 safety courses and seminars. Participants receive hands-on demonstrations for protective equipment. This year's event offers 45 safety courses and seminars. Oak Ridge, TN - This week, the Oak Ridge Office of

  13. Minnesota Agri-Power Project. Quarterly report, January--March, 1998

    SciTech Connect (OSTI)

    Wilbur, D.

    1998-05-01

    The Minnesota Valley Alfalfa Producers propose to build an alfalfa processing plant integrated with an advanced power plant system at the Granite Falls, Minnesota industrial park to provide 75 MW of base load electric power and a competitively priced source of value added alfalfa based products. This project utilizes air blown fluidized bed gasification technology to process alfalfa stems and another biomass to produce a hot, clean, low heating value gas that will be used in a gas turbine. Exhaust heat from the gas turbine will be used to generate steam to power a steam turbine and provide steam for the processing of the alfalfa leaf into a wide range of products including alfalfa leaf meal, a protein source for livestock. This progress report describes feedstock testing, feedstock supply system, performance guarantees, sales contracts, environmental permits, education, environment, economy, and project coordination and control.

  14. PRELIMINARY SURVEY OF U.S. STEEL CORPORATION--AGRI-CHEMICAL

    Office of Legacy Management (LM)

    status of those facilities utilized under Atomic Energy Commission (AEC) raw materials ... The 2 measurements consisted of garrma-ray exposure rates at a height of 1 m above the ...

  15. DOE STTR Phase I Final Technical Report For Agri-Tech Producers...

    Office of Scientific and Technical Information (OSTI)

    ... Product Output: .5 Tons per Hour Torrefied Product * Energy Content-lO,OOO BTUlb 5,500 kCalkg ( 10%) * Moisture Content < 10% * Input to Output Ratio: Approx. 3 tons of "green...

  16. An Independent Evaluation of Safety Culture at the U. S. Department...

    Office of Environmental Management (EM)

    ... and execute the actions that will result in improved safe and reliable performance. The safety culture components important for the existence of a healthy safety culture ...

  17. UTEX The Culture Collection of Algae at The University of Texas...

    Open Energy Info (EERE)

    UTEX The Culture Collection of Algae at The University of Texas at Austin Jump to: navigation, search Name: University of Texas at Austin The Culture Collection of Algae...

  18. Idaho National Laboratory Cultural Resource Management Annual Report FY 2006

    SciTech Connect (OSTI)

    Clayton F. Marler; Julie Braun; Hollie Gilbert; Dino Lowrey; Brenda Ringe Pace

    2007-04-01

    The Idaho National Laboratory Site is home to vast numbers and a wide variety of important cultural resources representing at least a 13,500-year span of human occupation in the region. As a federal agency, the Department of Energy Idaho Operations Office has legal responsibility for the management and protection of those resources and has delegated these responsibilities to its primary contractor, Battelle Energy Alliance (BEA). The INL Cultural Resource Management Office, staffed by BEA professionals, is committed to maintaining a cultural resource management program that accepts these challenges in a manner reflecting the resources importance in local, regional, and national history. This annual report summarizes activities performed by the INL Cultural Resource Management Office staff during Fiscal Year 2006. This work is diverse, far-reaching and though generally confined to INL cultural resource compliance, also includes a myriad of professional and voluntary community activities. This document is intended to be both informative to internal and external stakeholders, and to serve as a planning tool for future cultural resource management work to be conducted on the INL.

  19. Idaho National Laboratory Cultural Resource Management Annual Report FY 2007

    SciTech Connect (OSTI)

    Julie Braun; Hollie Gilbert; Dino Lowrey; Clayton Marler; Brenda Pace

    2008-03-01

    The Idaho National Laboratory (INL) Site is home to vast numbers and a wide variety of important cultural resources representing at least a 13,500-year span of human land use in the region. As a federal agency, the Department of Energy Idaho Operations Office has legal responsibility for the management and protection of those resources and has delegated these responsibilities to its primary contractor, Battelle Energy Alliance (BEA). The BEA professional staff is committed to maintaining a cultural resource management program that accepts these challenges in a manner reflecting the resources’ importance in local, regional, and national history. This annual report summarizes activities performed by the INL Cultural Resource Management Office (CRMO) staff during fiscal year 2007. This work is diverse, far-reaching and though generally confined to INL cultural resource compliance, also includes a myriad of professional and voluntary community activities. This document is intended to be both informative to internal and external stakeholders, and to serve as a planning tool for future cultural resource management work to be conducted on the INL.

  20. Low hardness organisms: Culture methods, sensitivities, and practical applications

    SciTech Connect (OSTI)

    DaCruz, A.; DaCruz, N.; Bird, M.

    1995-12-31

    EPA Regulations require biomonitoring of permitted effluent and stormwater runoff. Several permit locations were studied, in Virginia, that have supply water and or stormwater runoff which ranges in hardness from 5--30 mg/L. Ceriodaphnia dubia (dubia) and Pimephales promelas (fathead minnow) were tested in reconstituted water with hardnesses from 5--30 mg/L. Results indicated osmotic stresses present in the acute tests with the fathead minnow as well as chronic tests for the dubia and the fathead minnow. Culture methods were developed for both organism types in soft (30 mg) reconstituted freshwater. Reproductivity and development for each organisms type meets or exceeds EPA testing requirements for moderately hard organisms. Sensitivities were measured over an 18 month interval using cadmium chloride as a reference toxicant. Additionally, sensitivities were charted in contrast with those of organisms cultured in moderately hard water. The comparison proved that the sensitivities of both the dubia and the fathead minnow cultured in 30 mg water increased, but were within two standard deviations of the organism sensitivities of those cultured in moderately hard water. Latitude for use of organisms cultured in 30 mg was documented for waters ranging in hardness from 10--100 mg/L with no acclimation period required. The stability of the organism sensitivity was also validated. The application was most helpful in stormwater runoff and in effluents where the hardness was 30 mg/L or less.

  1. Hanford Cultural Resources Laboratory annual report for fiscal year 1989

    SciTech Connect (OSTI)

    Chatters, J.C.; Cadoret, N.A.; Minthorn, P.E.

    1990-06-01

    This report summarizes activities of the Hanford Cultural Resources Laboratory (HCRL) during fiscal year 1989. The HCRL provides support for managing the archaeological, historical, and cultural resources of the Hanford Site, Washington, in a manner consistent with the National Historic Preservation Act of 1966, the Archaeological Resources Protection Act of 1979, and the American Indian Religious Freedom Act of 1978. A major task in FY 1989 was completion and publication of the Hanford Cultural Resources Management Plan, which prioritizes tasks to be undertaken to bring the US Department of Energy -- Richland Operations into compliance with federal statutes, relations, and guidelines. During FY 1989, six tasks were performed. In order of priority, these were conducting 107 cultural resource reviews, monitoring the condition of 40 known prehistoric archaeological sites, assessing the condition of artifact collections from the Hanford Site, evaluating three sites and nominating two of those to the National Register of Historic Places, developing an education program and presenting 11 lectures to public organizations, and surveying approximately 1 mi{sup 2} of the Hanford Site for cultural resources. 7 refs., 4 figs., 4 tabs.

  2. Identifying Differences in Cultural Behavior in Online Groups

    SciTech Connect (OSTI)

    Gregory, Michelle L.; Engel, David W.; Bell, Eric B.; Mcgrath, Liam R.

    2012-07-23

    We have developed methods to identify online communities, or groups, using a combination of structural information variables and content information variables from weblog posts and their comments to build a characteristic footprint for groups. We have worked with both explicitly connected groups and 'abstract' groups, in which the connection between individuals is in interest (as determined by content based features) and behavior (metadata based features) as opposed to explicit links. We find that these variables do a good job at identifying groups, placing members within a group, and helping determine the appropriate granularity for group boundaries. The group footprint can then be used to identify differences between the online groups. In the work described here we are interested in determining how an individual's online behavior is influenced by their membership in more than one group. For example, individuals belong to a certain culture; they may belong as well to a demographic group, and other 'chosen' groups such as churches or clubs. There is a plethora of evidence surrounding the culturally sensitive adoption, use, and behavior on the Internet. In this work we begin to investigate how culturally defined internet behaviors may influence behaviors of subgroups. We do this through a series of experiments in which we analyze the interaction between culturally defined behaviors and the behaviors of the subgroups. Our goal is to (a) identify if our features can capture cultural distinctions in internet use, and (b) determine what kinds of interaction there are between levels and types of groups.

  3. INTEGRATED SAFETY MANAGEMENT SYSTEM SAFETY CULTURE IMPROVEMENT INITIATIVE

    SciTech Connect (OSTI)

    MCDONALD JA JR

    2009-01-16

    In 2007, the Department of Energy (DOE) identified safety culture as one of their top Integrated Safety Management System (ISMS) related priorities. A team was formed to address this issue. The team identified a consensus set of safety culture principles, along with implementation practices that could be used by DOE, NNSA, and their contractors. Documented improvement tools were identified and communicated to contractors participating in a year long pilot project. After a year, lessons learned will be collected and a path forward determined. The goal of this effort was to achieve improved safety and mission performance through ISMS continuous improvement. The focus of ISMS improvement was safety culture improvement building on operating experience from similar industries such as the domestic and international commercial nuclear and chemical industry.

  4. Hanford Cultural Resources Laboratory annual report for fiscal year 1994

    SciTech Connect (OSTI)

    Nickens, P.R.; Wright, M.K.; Cadoret, N.A.; Dawson, M.V.; Harvey, D.W.; Simpson, E.M.

    1995-09-01

    The Hanford Site occupies 560 sq. miles of land along the Columbia River in SE Washington. The Hanford Reach of the river is one of the most archaeologically rich areas in the western Columbia Plateau. To manage the Hanford Site`s archaeological, historical, and cultural resources, the Hanford Cultural Resources Laboratory (HCRL) was established in 1987. HCRL ensures DOE complies with federal statutes, regulations, and guidelines. In FY 1994, HCRL conducted cultural resource reviews, conducted programs to identify and monitor historic and archaeological sites, etc. HCRL staff conducted 511 reviews, 29 of which required archaeological surveys and 10 of which required building documentation. Six prehistoric sites, 23 historic sites, one paleontological site, and two sites with historic and prehistoric components were discovered.

  5. INEEL Cultural Resource Management Program Annual Report - 2004

    SciTech Connect (OSTI)

    Clayton F. Marler

    2005-01-01

    As a federal agency, the U.S. Department of Energy has been directed by Congress, the U.S. president, and the American public to provide leadership in the preservation of prehistoric, historic, and other cultural resources on the lands it administers. This mandate to preserve cultural resources in a spirit of stewardship for the future is outlined in various federal preservation laws, regulations, and guidelines such as the National Historic Preservation Act, the Archaeological Resources Protection Act, and the National Environmental Policy Act. The Idaho National Engineering and Environmental Laboratory Site is located in southeastern Idaho, and is home to vast numbers and a wide variety of important cultural resources representing at least 13,000-year span of human occupation in the region. These resources are nonrenewable, bear valuable physical and intangible legacies, and yield important information about the past, present, and perhaps the future. There are special challenges associated with balancing the preservation of these resources with the management and ongoing operation of an active scientific laboratory, while also cleaning up the waste left by past programs and processes. The Department of Energy Idaho Operations Office has administrative responsibility for most of the Site, excluding lands and resources managed by the Naval Reactors Facility and (in 2004) Argonne National Laboratory-West. The Department of Energy is committed to a cultural resource program that accepts these challenges in a manner reflecting both the spirit and intent of the legislative requirements. This annual report is an overview of Cultural Resource Management Program activities conducted during Fiscal Year 2004 and is intended to be both informative to external stakeholders and to serve as a planning tool for future cultural resource management work to be conducted on the Site.

  6. 7th Workshop on Risk Informed Regulation and Safety Culture

    Office of Energy Efficiency and Renewable Energy (EERE)

    The 7th Workshop on Risk Informed Regulation and Safety Culture was one of a series of workshops designed by the Office of Nuclear Energy in concert with experts from the Idaho National Laboratory to assist China in developing a comprehensive, successful and efficient Risk-Informed Regulatory framework.

  7. Guidelines for Evaluating and Documenting Traditional Cultural Properties

    Broader source: Energy.gov [DOE]

    This National Park Service bulletin is meant to assist federal agencies, State Historic Preservation Officers, local governments, Indian tribes, and other historic preservation practitioners in determining whether properties thought to have traditional cultural significance are eligible for inclusion in the National Register of Historic Places.

  8. Method and system of culturing an algal mat

    DOE Patents [OSTI]

    Das, Keshav C; Cannon, Benjamin R; Bhatnagar, Ashish; Chinnasamy, Senthil

    2014-05-13

    A system and method for culturing algae are presented. The system and method utilize a fog of growth medium that is delivered to an algal mat generator along with a stream of CO.sub.2 to promote growth of algal cells contained in the generator.

  9. Guidelines for Evaluating and Documenting Traditional Cultural Properties (NPS, 1998)

    Broader source: Energy.gov [DOE]

    This National Park Service bulletin is meant to assist federal agencies, State Historic Preservation Officers, local governments, Indian tribes, and other historic preservation practitioners in determining whether properties thought to have traditional cultural significance are eligible for inclusion in the National Register of Historic Places.

  10. CULTURAL RESOURCE MANAGEMENT PLAN FOR BROOKHAVEN NATIONAL LABORATORY.

    SciTech Connect (OSTI)

    DAVIS, M.

    2005-04-01

    The Cultural Resource Management Plan (CRMP) for Brookhaven National Laboratory (BNL) provides an organized guide that describes or references all facets and interrelationships of cultural resources at BNL. This document specifically follows, where applicable, the format of the U.S. Department of Energy (DOE) Environmental Guidelines for Development of Cultural Resource Management Plans, DOE G 450.1-3 (9-22-04[m1]). Management strategies included within this CRMP are designed to adequately identify the cultural resources that BNL and DOE consider significant and to acknowledge associated management actions. A principal objective of the CRMP is to reduce the need for additional regulatory documents and to serve as the basis for a formal agreement between the DOE and the New York State Historic Preservation Officer (NYSHPO). The BNL CRMP is designed to be a ''living document.'' Each section includes identified gaps in the management plan, with proposed goals and actions for addressing each gap. The plan will be periodically revised to incorporate new documentation.

  11. Sandia National Laboratories: Careers: Life at Sandia: People and Culture

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    People and Culture Solar cell picture Quality people, quality work Integrity, excellence, and exceptional service in the national interest. At Sandia, creativity, multidisciplinary thought, and diverse ideas are encouraged. Our unique work requires the collective minds of the nation's top scientists, engineers, and support staff. Each year, Sandians are recognized for developing a range of breakthrough technologies with commercial applications of global importance. Sandia embraces diversity,

  12. Cultural analysis: The missing factor in root-cause evaluation

    SciTech Connect (OSTI)

    Lamb, H.

    1989-01-01

    The purpose of this paper is to provide a model that can focus attention on appropriate cultural targets of inquiry, provide a completion criterion for root-cause completeness, and illustrate results. The illustration provided is as follows: Discover the root causes(s) related to issues of a nuclear reactor operator sleeping, inattention to duties, failure to adhere to procedures, and management inaction or adequate action.

  13. Co-cultured Synechococcus and Shewanella Produce Hydrocarbons without

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cellulosic Feedstock - Energy Innovation Portal Co-cultured Synechococcus and Shewanella Produce Hydrocarbons without Cellulosic Feedstock DOE Grant Recipients University of Minnesota Contact University of Minnesota About This Technology <span id="Caption"><span id="ctl00_MainContentHolder_zoomimage_defaultCaption">Shewanella Oneidensis naturally produces hydrocarbons without cellulosic feedstock.</span></span> Shewanella Oneidensis naturally

  14. Nissan Showcases the Results of an Energy-Wise Corporate Culture

    SciTech Connect (OSTI)

    2010-06-11

    The corporate leadership at Nissan cultivates a culture of energy efficiency, encouraging employees to practice good energy management at work and in every part of their lives. Read about Nissan's energy-conscience culture.

  15. Microsoft PowerPoint - January Safety Culture.pptx [Read-Only]

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Culture Presentation to Hanford Advisory Board Steve Pfaff February 9, 2012 Safety Culture * Safety culture is an organization's values and behaviors modeled by its leaders and internalized by its members, which serve to make safe performance of work the to make safe performance of work the overriding priority to protect the workers, public, and the environment. (DOE ISMS Guide, DOE G 450.4-1C) ORP is Not Alone! Safety is Everyone's responsibility * Who helps us in our safety culture efforts? -

  16. Microsoft Word - 2015 12 7 Organizational Safety Culture - HAB Advice first draft Rev 1.....docx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    COMMITTEE DISCUSSION Hanford Advisory Board Advice: Testing Safety Culture in Practice Lead Issue Managers: Dirk Dunning and Liz Mattson Background The Hanford Advisory Board (HAB, Board) has been focused on reviewing, discussing, and issuing advice on safety culture since the Defense Nuclear Facilities Safety Board issued its Recommendation 2011-1: Safety Culture at the Waste Treatment and Immobilization Plant in June 2011. Safety culture terminology has created confusion in our discussions, as

  17. Establishing and Promoting a Culture of Safety in Chemistry Laboratory Research

    SciTech Connect (OSTI)

    Fryberger, Teresa

    2014-12-23

    Final technical/scientific report for the project, Establishing and Promoting a Culture of Safety in Chemistry Laboratory Research.

  18. INL Cultural Resource Monitoring Report for FY 2015

    SciTech Connect (OSTI)

    Pace, Brenda Ringe; Olson, Christina Liegh; Gilbert, Hollie Kae; Holmer, Marie Pilkington

    2015-10-01

    This report describes the cultural resource monitoring activities of the Idaho National Laboratory’s (INL) Cultural Resource Management (CRM) Office during fiscal year (FY) 2015. Throughout the year, 67 total monitoring visits were completed, with several especially sensitive resources visited on more than one occasion. Overall, FY 2015 monitoring included surveillance of the following 49 individual cultural resource localities: three locations with human remains, one of which is also a cave; nine additional caves; twenty prehistoric archaeological sites; five historic archaeological sites; two historic trails; Experimental Breeder Reactor I (EBR-I), a National Historic Landmark; Aircraft Nuclear Propulsion (ANP) objects located at EBR-I; and eight Arco Naval Proving Ground (NPG) property types. Several INL work processes and projects were also monitored to confirm compliance with original INL CRM recommendations and assess the effects of ongoing work. On two occasions, ground disturbing activities within the boundaries of the Power Burst Facility/Critical Infrastructure Test Range Complex (PBF/CITRC) were observed by INL CRM staff prepared to respond to any additional finds of Native American human remains. Finally, the current location housing INL Archives and Special Collections was evaluated once. Most of the cultural resources monitored in FY 2015 exhibited no adverse impacts, resulting in Type 1 impact assessments. However, Type 2 impacts were noted 13 times. In one case, a portion of a historic trail was graded without prior review or coordination with the INL CRM Office, resulting in impacts to the surface of the trail and one archaeological site. Evidence of unauthorized artifact collection/ looting was also documented at three archaeological sites located along INL powerlines. Federal agents concluded a FY 2012 investigation by filing civil charges and levying fine under the Archaeological Resource Protection Act against one INL employee for this kind

  19. Why is it so difficult to grow fuelwood

    SciTech Connect (OSTI)

    Noronha, R.

    1981-01-01

    Examples of successful and unsuccessful woodlot programmes are cited from China, Korea, India, Tanzania and Niger and the role of social factors examined. Effective village forestry involves social, cultural, economic and local political factors. (Refs. 15).

  20. Practitioners, professional cultures, and perceptions of impact assessment

    SciTech Connect (OSTI)

    Morgan, Richard K.; Hart, Andrew; Freeman, Claire; Coutts, Brian; Colwill, David; Hughes, Andrew

    2012-01-15

    The very nature of impact assessment (IA) means that it often involves practitioners from a very wide range of disciplinary and professional backgrounds, which open the possibility that how IA is perceived and practised may vary according to the professional background of the practitioner. The purpose of this study is to investigate the extent to which a practitioner's professional background influences their perceptions of the adequacy of impact assessment in New Zealand under the Resource Management Act (RMA). Information gathered concerned professional affiliations, training, understanding of impact assessment practise, and perceptions of adequacy in relation to impact assessment. The results showed a dominance of a legalistic, operational perspective of impact assessment under the Resource Management Act, across all the main professions represented in the study. However, among preparers of impact assessments there was clear evidence of differences between the four main professional groups - surveyors, planners, engineers and natural scientists - in the way they see the nature and purpose of impact assessment, the practical steps involved, and what constitutes adequacy. Similarly, impact assessment reviewers - predominantly planners and lawyers - showed variations in their expectations of impact assessment depending on their respective professional affiliation. Although in many cases the differences seem to be more of a matter of emphasis, rather than major disputes on what constitutes a good process, even those differences can add up to rather distinct professional cultures of impact assessment. The following factors are seen as leading to the emergence of such professional cultures: different professions often contribute in different ways to an impact assessment, affecting their perception of the nature and purpose of the process; impact assessment training will usually be a secondary concern, compared with the core professional training, which will be

  1. Spectroradiometric monitoring for open outdoor culturing of algae and cyanobacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Reichardt, Thomas A.; Collins, Aaron M.; McBride, Robert C.; Behnke, Craig A.; Timlin, Jerilyn A.

    2014-08-20

    We assess the measurement of hyperspectral reflectance for the outdoor monitoring of green algae and cyanobacteria cultures with a multi-channel, fiber-coupled spectroradiometer. Reflectance data acquired over a four-week period are interpreted via numerical inversion of a reflectance model, in which the above-water reflectance is expressed as a quadratic function of the single backscattering albedo, dependent on the absorption and backscatter coefficients. The absorption coefficient is treated as the sum of component spectra consisting of the cultured species (green algae or cyanobacteria), dissolved organic matter, and water (including the temperature dependence of the water absorption spectrum). The backscatter coefficient is approximatedmore » as the scaled Hilbert transform of the culture absorption spectrum with a wavelength-independent vertical offset. Additional terms in the reflectance model account for the pigment fluorescence features and the water surface reflection of sunlight and skylight. For both the green algae and cyanobacteria, the wavelength-independent vertical offset of the backscatter coefficient is found to scale linearly with daily dry weight measurements, providing the capability for a non-sampling measurement of biomass in outdoor ponds. Other fitting parameters in the reflectance model are compared to auxiliary measurements and physics-based calculations. The magnitudes of the sunlight and skylight water-surface contributions derived from the reflectance model compare favorably with Fresnel reflectance calculations, while the reflectance-derived quantum efficiency of Chl-a fluorescence is found to be in agreement with literature values. To conlclude, the water temperature derived from the reflectance model exhibits excellent agreement with thermocouple measurements during the morning hours and highlights significantly elevated temperatures in the afternoon hours.« less

  2. Spectroradiometric monitoring for open outdoor culturing of algae and cyanobacteria

    SciTech Connect (OSTI)

    Reichardt, Thomas A.; Collins, Aaron M.; McBride, Robert C.; Behnke, Craig A.; Timlin, Jerilyn A.

    2014-08-20

    We assess the measurement of hyperspectral reflectance for the outdoor monitoring of green algae and cyanobacteria cultures with a multi-channel, fiber-coupled spectroradiometer. Reflectance data acquired over a four-week period are interpreted via numerical inversion of a reflectance model, in which the above-water reflectance is expressed as a quadratic function of the single backscattering albedo, dependent on the absorption and backscatter coefficients. The absorption coefficient is treated as the sum of component spectra consisting of the cultured species (green algae or cyanobacteria), dissolved organic matter, and water (including the temperature dependence of the water absorption spectrum). The backscatter coefficient is approximated as the scaled Hilbert transform of the culture absorption spectrum with a wavelength-independent vertical offset. Additional terms in the reflectance model account for the pigment fluorescence features and the water surface reflection of sunlight and skylight. For both the green algae and cyanobacteria, the wavelength-independent vertical offset of the backscatter coefficient is found to scale linearly with daily dry weight measurements, providing the capability for a non-sampling measurement of biomass in outdoor ponds. Other fitting parameters in the reflectance model are compared to auxiliary measurements and physics-based calculations. The magnitudes of the sunlight and skylight water-surface contributions derived from the reflectance model compare favorably with Fresnel reflectance calculations, while the reflectance-derived quantum efficiency of Chl-a fluorescence is found to be in agreement with literature values. To conlclude, the water temperature derived from the reflectance model exhibits excellent agreement with thermocouple measurements during the morning hours and highlights significantly elevated temperatures in the afternoon hours.

  3. WTP Safety Culture Advice Joint Topic (HSEP/TWC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    January 2012 v1 Page 1 of 2 Note: The views expressed in committee meetings should not be considered a substitute for full HAB consensus on any particular issue. WTP Safety Culture Advice Joint Topic (HSEP/TWC) Framing questions for discussion regarding DOE's recently released Implementation Plan: Re: Secretary Chu's response to the DNFSB with the Implementation Plan for Recommendation 2011-1 (December 27, 2011) Note: The ORP coordinator for the DOE Response to DNFSB 2011-1 is Steve Pfaff. o

  4. Culture, and a Metrics Methodology for Biological Countermeasure Scenarios

    SciTech Connect (OSTI)

    Simpson, Mary J.

    2007-03-15

    Outcome Metrics Methodology defines a way to evaluate outcome metrics associated with scenario analyses related to biological countermeasures. Previous work developed a schema to allow evaluation of common elements of impacts across a wide range of potential threats and scenarios. Classes of metrics were identified that could be used by decision makers to differentiate the common bases among disparate scenarios. Typical impact metrics used in risk calculations include the anticipated number of deaths, casualties, and the direct economic costs should a given event occur. There are less obvious metrics that are often as important and require more intensive initial work to be incorporated. This study defines a methodology for quantifying, evaluating, and ranking metrics other than direct health and economic impacts. As has been observed with the consequences of Hurricane Katrina, impacts to the culture of specific sectors of society are less obvious on an immediate basis but equally important over the ensuing and long term. Culture is used as the example class of metrics within which • requirements for a methodology are explored • likely methodologies are examined • underlying assumptions for the respective methodologies are discussed • the basis for recommending a specific methodology is demonstrated. Culture, as a class of metrics, is shown to consist of political, sociological, and psychological elements that are highly valued by decision makers. In addition, cultural practices, dimensions, and kinds of knowledge offer complementary sets of information that contribute to the context within which experts can provide input. The quantification and evaluation of sociopolitical, socio-economic, and sociotechnical impacts depend predominantly on subjective, expert judgment. Epidemiological data is limited, resulting in samples with statistical limits. Dose response assessments and curves depend on the quality of data and its relevance to human modes of exposure

  5. Ion beam analysis in cultural heritage studies: Milestones and perspectives

    SciTech Connect (OSTI)

    Dran, Jean-Claude; Calligaro, Thomas

    2013-07-18

    For three decades, ion beam analysis (IBA) in external mode was considered as the best choice for the characterisation of cultural heritage materials, as it combines excellent analytical performance and non-invasive character. However, in recent years, other analytical techniques arose as serious competitors, such as those based on synchrotron radiation (X-ray absorption, fluorescence or diffraction) or those using portable instruments (XRF, micro-Raman). It is shown that nevertheless IBA remains unmatched thanks to two unique features, namely the analysis of light elements and the high-resolution 3D chemical imaging.

  6. American Material Culture: Investigating a World War II Trash Dump

    SciTech Connect (OSTI)

    Julie Braun

    2005-10-01

    The Idaho National Laboratory: An Historical Trash Trove Historians and archaeologists love trash, the older the better. Sometimes these researchers find their passion in unexpected places. In this presentation, the treasures found in a large historic dump that lies relatively untouched in the middle of the Idaho National Laboratory (INL) will be described. The U.S. military used the central portion of the INL as one of only six naval proving grounds during World War II. They dumped trash in dry irrigation canals during and after their wartime activities and shortly before the federal government designated this arid and desolate place as the nation’s nuclear reactor testing station in 1949. When read critically and combined with memories and photographs, the 60-year old trash provides a glimpse into 1940s’ culture and the everyday lives of ordinary people who lived and worked during this time on Idaho’s desert. Thanks to priceless stories, hours of research, and the ability to read the language of historic artifacts, the dump was turned from just another trash heap into a treasure trove of 1940s memorabilia. Such studies of American material culture serve to fire our imaginations, enrich our understanding of past practices, and humanize history. Historical archaeology provides opportunities to integrate inanimate objects with animated narrative and, the more recent the artifacts, the more human the stories they can tell.

  7. American Material Culture: Investigating a World War II Trash Dump

    SciTech Connect (OSTI)

    Julie Braun

    2005-10-01

    The Idaho National Laboratory: An Historical Trash Trove Historians and archaeologists love trash, the older the better. Sometimes these researchers find their passion in unexpected places. In this presentation, the treasures found in a large historic dump that lies relatively untouched in the middle of the Idaho National Laboratory (INL) will be described. The U.S. military used the central portion of the INL as one of only six naval proving grounds during World War II. They dumped trash in dry irrigation canals during and after their wartime activities and shortly before the federal government designated this arid and desolate place as the nations nuclear reactor testing station in 1949. When read critically and combined with memories and photographs, the 60-year old trash provides a glimpse into 1940s culture and the everyday lives of ordinary people who lived and worked during this time on Idahos desert. Thanks to priceless stories, hours of research, and the ability to read the language of historic artifacts, the dump was turned from just another trash heap into a treasure trove of 1940s memorabilia. Such studies of American material culture serve to fire our imaginations, enrich our understanding of past practices, and humanize history. Historical archaeology provides opportunities to integrate inanimate objects with animated narrative and, the more recent the artifacts, the more human the stories they can tell.

  8. Safety Culture and Best Practices at Japan's Fusion Research Facilities

    SciTech Connect (OSTI)

    Rule, Keith

    2014-05-01

    The Safety Monitor Joint Working Group (JWG) is one of the magnetic fusion research collaborations between the US Department of Energy and the government of Japan. Visits by occupational safety personnel are made to participating institutions on a biennial basis. In the 2013 JWG visit of US representatives to Japan, the JWG members noted a number of good safety practices in the safety walkthroughs. These good practices and safety culture topics are discussed in this paper. The JWG hopes that these practices for worker safety can be adopted at other facilities. It is a well-known, but unquantified, safety principle that well run, safe facilities are more productive and efficient than other facilities (Rule, 2009). Worker safety, worker productivity, and high quality in facility operation all complement each other (Mottel, 1995).

  9. Safety Culture And Best Practices At Japan's Fusion Research Facilities

    SciTech Connect (OSTI)

    Rule, K.; King, M.; Takase, Y.; Oshima, Y.; Nishimura, K.; Sukegawa, A.

    2014-04-01

    The Safety Monitor Joint Working Group (JWG) is one of the magnetic fusion research collaborations between the US Department of Energy and the government of Japan. Visits by occupational safety personnel are made to participating institutions on a biennial basis. In the 2013 JWG visit of US representatives to Japan, the JWG members noted a number of good safety practices in the safety walkthroughs. These good practices and safety culture topics are discussed in this paper. The JWG hopes that these practices for worker safety can be adopted at other facilities. It is a well-known, but unquantified, safety principle that well run, safe facilities are more productive and efficient than other facilities (Rule, 2009). Worker safety, worker productivity, and high quality in facility operation all complement each other (Mottel, 1995).

  10. Getting on the same page-Safety Culture | Y-12 National Security Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Getting on the same ... Getting on the same page-Safety Culture Posted: July 21, 2015 - 5:27pm Safety culture encompasses our core values, attitudes and behaviors that emphasize safety - the protection of people and the environment - as the overriding priority of our work. The National Nuclear Security Administration Production Office and Consolidated Nuclear Security are committed to having a strong safety culture. The combined core values of NPO and CNS - trust, excellence, teamwork,

  11. Review of Nuclear Safety Culture at the Hanford Site Waste Treatment and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Immobilization Plant Project, October 2010 | Department of Energy Review of Nuclear Safety Culture at the Hanford Site Waste Treatment and Immobilization Plant Project, October 2010 Review of Nuclear Safety Culture at the Hanford Site Waste Treatment and Immobilization Plant Project, October 2010 October 2010 Report for independent review of the nuclear safety culture at the Waste Treatment and Immobilization Plant (WTP) project at DOE's Hanford Site. This report provides the results of a

  12. Using Operational Security (OPSEC) to Support a Cyber Security Culture in

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Control Systems Environments | Department of Energy Using Operational Security (OPSEC) to Support a Cyber Security Culture in Control Systems Environments Using Operational Security (OPSEC) to Support a Cyber Security Culture in Control Systems Environments This document reviews several key operational cyber security elements that are important for control systems and industrial networks and how those elements can drive the creation of a cyber security-sensitive culture. Using Operational

  13. DISPELLING MYTHS AND MISCONCEPTIONS TO IMPLEMENT A SAFETY CULTURE

    SciTech Connect (OSTI)

    Potts, T. Todd; Smith, Ken; Hylko, James M.

    2003-02-27

    Industrial accidents are typically reported in terms of technological malfunctions, ignoring the human element in accident causation. However, over two-thirds of all accidents are attributable to human and organizational factors (e.g., planning, written procedures, job factors, training, communication, and teamwork), thereby affecting risk perception, behavior and attitudes. This paper reviews the development of WESKEM, LLC's Environmental, Safety, and Health (ES&H) Program that addresses human and organizational factors from a top-down, bottom-up approach. This approach is derived from the Department of Energy's Integrated Safety Management System. As a result, dispelling common myths and misconceptions about safety, while empowering employees to ''STOP work'' if necessary, have contributed to reducing an unusually high number of vehicle, ergonomic and slip/trip/fall incidents successfully. Furthermore, the safety culture that has developed within WESKEM, LLC's workforce consists of three common characteristics: (1) all employees hold safety as a value; (2) each individual feels responsible for the safety of their co-workers as well as themselves; and (3) each individual is willing and able to ''go beyond the call of duty'' on behalf of the safety of others. WESKEM, LLC as a company, upholds the safety culture and continues to enhance its existing ES&H program by incorporating employee feedback and lessons learned collected from other high-stress industries, thereby protecting its most vital resource - the employees. The success of this program is evident by reduced accident and injury rates, as well as the number of safe work hours accrued while performing hands-on field activities. WESKEM, LLC (Paducah + Oak Ridge) achieved over 800,000 safe work hours through August 2002. WESKEM-Paducah has achieved over 665,000 safe work hours without a recordable injury or lost workday case since it started operations on February 28, 2000.

  14. DOE Policy 141.1: Management of Cultural Resources (DOE, 2001...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    to raise the level of awareness and accountability among DOE contractors concerning the importance of the Department's cultural resource-related legal and trust responsibilities. ...

  15. Nissan Showcases the Results of an Energy-Wise Corporate Culture |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Showcases the Results of an Energy-Wise Corporate Culture Nissan Showcases the Results of an Energy-Wise Corporate Culture This case study describes the benefits of Nissan instilling a deep-rooted, company-wide culture of energy efficiency and how it informed long-term success in energy management and plant-wide energy performance. Nissan Showcases the Results of an Energy-Wise Corporate Culture (June 2010) (1.3 MB) More Documents & Publications Nissan North America:

  16. Environmental Guidelines for Development of Cultural Resource Management Plans--Update

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2004-09-22

    This Guide provides guidelines for the development of an individual Cultural Resource Management Plan for each DOE facility and program. Canceled by DOE N 251.82.

  17. NMSA 18-6-9 - Cultural Properties Act | Open Energy Information

    Open Energy Info (EERE)

    Properties ActLegal Abstract Governs excavation of, injury or destruction to, and criminal damage to cultural property. Published NA Year Signed or Took Effect 1993 Legal...

  18. Integrating community perceptions and cultural diversity in social impact assessment in Nigeria

    SciTech Connect (OSTI)

    Nzeadibe, Thaddeus Chidi; Ajaero, Chukwuedozie Kelechukwu; Okonkwo, Emeka Emmanuel; Okpoko, Patrick Uche; Akukwe, Thecla Iheoma; Njoku-Tony, Roseline Feechi

    2015-11-15

    The Environmental Impact Assessment (EIA) Act of 1992 aimed to make the environment a central theme in development in Nigeria. Nevertheless, the extent of engagement with local cultures in the Nigerian EIA process is not statutorily guaranteed. While most EIAs in Nigeria have been for oil and gas projects in the Niger Delta, and have focused strongly on the biophysical environment, socio-economic and cultural aspects have remained marginal. The palpable neglect of community perceptions and cultural diversity in social impact assessment (SIA) in this region prone to conflict has tended to alienate the people in the decision-making process. Thus, despite claims to compliance with regulatory requirements for EIAs, and numerous purported sustainable development initiatives by international oil companies (IOCs), the region continues to face multiple sustainability challenges. This paper situates local perceptions and cultural diversity in participatory development and canvasses the integration of community perceptions and cultural diversity into SIA in the Niger Delta region. It is argued that doing this would be critical to ensuring acceptance and success of development actions within the context of local culture while also contributing to sustainable development policy in the region. - Highlights: • Nigeria EIA Act aimed to make the environment central to development in Nigeria. • Engagement with local communities in the process is not statutorily guaranteed. • SIAs in Nigeria neglect community perceptions and cultural diversity. • Article canvasses integrating community perceptions and cultural diversity in SIA. • Local acceptance in context of culture would yield sustainable development outcomes.

  19. Use of co-cultures in the production of ethanol by the fermentation of biomass

    SciTech Connect (OSTI)

    Zeikus, J.G.; Ben-Bassat, A.; Lamed, R.J.; Ng, T.K.

    1983-08-23

    Production of ethanol and enzymes is disclosed by fermentation of biomass with co-cultures of C. thermocellum and C. thermohydrosulfuricum.

  20. Integration of Safety Culture Attributes into the EFCOG WP&C Program Guideline Document

    Broader source: Energy.gov [DOE]

    Slide Presentation by Steele Coddington, NSTec, Las Vegas and John McDonald, WRPS, Hanford. Integration of Safety Culture Attributes into EFCOG Work Planning and Control Guidance Document.

  1. DOE Policy 141.1: Management of Cultural Resources (2001) | Department...

    Broader source: Energy.gov (indexed) [DOE]

    (DOE) programs and field elements integrate cultural resources management into their missions and activities and to raise the level of awareness and accountability among DOE ...

  2. Review of Nuclear Safety Culture at the Hanford Site Waste Treatment...

    Energy Savers [EERE]

    Treatment and Immobilization Plant Project, October 2010 Review of Nuclear Safety Culture at the Hanford Site Waste Treatment and Immobilization Plant Project, October 2010 October ...

  3. Apical polarity in three-dimensional culture systems: where to now?

    SciTech Connect (OSTI)

    Inman, J.L.; Bissell, Mina

    2010-01-21

    Delineation of the mechanisms that establish and maintain the polarity of epithelial tissues is essential to understanding morphogenesis, tissue specificity and cancer. Three-dimensional culture assays provide a useful platform for dissecting these processes but, as discussed in a recent study in BMC Biology on the culture of mammary gland epithelial cells, multiple parameters that influence the model must be taken into account.

  4. Bone morphogenetic protein-induced cartilage development in tissue culture

    SciTech Connect (OSTI)

    Sato, K.; Urist, M.R.

    1984-03-01

    Outgrowths of mesenchyme-type cells from explants of allogeneic rat muscle onto a substratum of bone matrix containing bone morphogenetic protein (BMP) differentiate into cartilage. When BMP is chemically extracted from the bone matrix, the explanted cells develop only into fibrous tissue. When exogenous bovine BMP is introduced into the culture medium, either as a microsuspension or as a layer of particles between the matrix and the muscle cell tissue, cartilage develops at the interface between the matrix and the mesenchymal cell outgrowth. The chondrogenetic response is induced by as little as 2 micrograms of BMP; the optimum dose is 10 micrograms/40 mg (wet weight) of explant. The endogenous BMP equivalent for a comparable chondrogenetic response is about 0.6 micrograms/mg of allogeneic matrix. The minimum time for transfer of BMP to mesenchymal cell receptors is 1.0 hour, adequate time is 2.5 hours, and optimum time is approximately 5.0 hours. Measured in terms of incorporation of /sup 3/H-thymidine into DNA and of /sup 35/S sulfate into glycosaminoglycan, there is a latent period of one to three days preceeding the differentiation of mesenchyme-type cells into cartilage. During this latent period BMP-modulated mesenchymal cells disaggregate, migrate, reaggregate, and proliferate on new surfaces and constitute the morphogenetic phase of bone development. By the fourth day cells simultaneously undergo mitotic division, synthesize extracellular cartilage matrix, and establish the cytodifferentiation phase of development.

  5. Specific albumin binding to microvascular endothelium in culture

    SciTech Connect (OSTI)

    Schnitzer, J.E.; Carley, W.W.; Palade, G.E. )

    1988-03-01

    The specific binding of rat serum albumin (RSA) to confluent microvascular endothelial cells in culture derived from the vasculature of the rat epididymal fat pad was studied at 4{degree}C by radioassay and immunocytochemistry. Radioiodinated RSA ({sup 125}I-RSA) binding to the cells reached equilibrium at {approximately} 20 min incubation. Albumin binding was a slowly saturating function over concentrations ranging from 0.01 to 50 mg/ml. Specific RSA binding with a moderate apparent affinity constant of 1.0 mg/ml and with a maximum binding concentration of 90 ng/cm{sup 2} was immunolocalized with anti-RSA antibody to the outer (free) side of the enothelium. Scatchard analysis of the binding yielded a nonlinear binding curve with a concave-upward shape. Dissociation rate analysis supports negative cooperativity of albumin binding, but multiple binding sites may also be present. Albumin binding fulfilled many requirements for ligand specificity including saturability, reversibility, competibility, and dependence on both cell type and cell number. The results are discussed in terms of past in situ investigations on the localization of albumin binding to vascular endothelium and its effect on transendothelial molecular transport.

  6. Phenylpropanoid metabolism in suspension cultures of Vanilla planifolia Andr

    SciTech Connect (OSTI)

    Funk, C.; Brodelius, P.E. )

    1990-09-01

    Feeding of cinnamic acid and ferulic acid to non-treated and chitosan-treated cell suspension cultures of Vanilla planifolia resulted in the formation of trace amounts of p-hydroxybenzoic acid (5.2 micrograms per gram fresh weight of cells) and vanillic acid (6.4 micrograms per gram fresh weight of cells), respectively. Addition of a 4-hydroxycinnamate: CoA-ligase inhibitor, 3,4-(methylenedioxy)-cinnamic acid (MDCA), resulted in a reduced biosynthesis of ligneous material with a simultaneous significant increased vanillic acid formation (around 75 micrograms per gram fresh weight of cells). A K{sub i} of 100 micromolar for 4-hydroxycinnamate: CoA-ligase in a crude preparation was estimated for this inhibitor. It is suggested that the conversion of cinnamic acids into benzoic acids does not involve cinnamoyl CoA esters as intermediates. Feeding of {sup 14}C-cinnamic acid and {sup 14}C-ferulic acid to cells treated with MDCA indicate that cinnamic acid, but not ferulic acid, is a precursor of vanillic acid in these cultivated cells of V. planifolia.

  7. Safety culture management: The importance of organizational factors

    SciTech Connect (OSTI)

    Haber, S.B.; Shurberg, D.A.; Jacobs, R.; Hofmann, D.

    1995-05-01

    The concept of safety culture has been used extensively to explain the underlying causes of performance based events, both positive and negative, across the nuclear industry. The work described in this paper represents several years of effort to identify, define and assess the organizational factors important to safe performance in nuclear power plants (NPPs). The research discussed in this paper is primarily conducted in support of the US Nuclear Regulatory Commission`s (NRC) efforts in understanding the impact of organizational performance on safety. As a result of a series of research activities undertaken by numerous NRC contractors, a collection of organizational dimensions has been identified and defined. These dimensions represent what is believed to be a comprehensive taxonomy of organizational elements that relate to the safe operation of nuclear power plants. Techniques were also developed by which to measure these organizational dimensions, and include structured interview protocols, behavioral checklists, and behavioral anchored rating scales (BARS). Recent efforts have focused on devising a methodology for the extraction of information related to the identified organizational dimensions from existing NRC documentation. This type of effort would assess the applicability of the organizational dimensions to existing NRC inspection and evaluation reports, refine the organizational dimensions previously developed so they are more relevant to the task of retrospective analysis, and attempt to rate plants based on the review of existing NRC documentation using the techniques previously developed for the assessment of organizational dimensions.

  8. Quantifying Stochastic Noise in Cultured Circadian Reporter Cells

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    John, Peter C.; Doyle, III, Francis J.

    2015-11-20

    We report that stochastic noise at the cellular level has been shown to play a fundamental role in circadian oscillations, influencing how groups of cells entrain to external cues and likely serving as the mechanism by which cell-autonomous rhythms are generated. Despite this importance, few studies have investigated how clock perturbations affect stochastic noise—even as increasing numbers of high-throughput screens categorize how gene knockdowns or small molecules can change clock period and amplitude. This absence is likely due to the difficulty associated with measuring cell-autonomous stochastic noise directly, which currently requires the careful collection and processing of single-cell data. Inmore » this study, we show that the damping rate of population-level bioluminescence recordings can serve as an accurate measure of overall stochastic noise, and one that can be applied to future and existing high-throughput circadian screens. Using cell-autonomous fibroblast data, we first show directly that higher noise at the single-cell results in faster damping at the population level. Next, we show that the damping rate of cultured cells can be changed in a dose-dependent fashion by small molecule modulators, and confirm that such a change can be explained by single-cell noise using a mathematical model. We further demonstrate the insights that can be gained by applying our method to a genome-wide siRNA screen, revealing that stochastic noise is altered independently from period, amplitude, and phase. Finally, we hypothesize that the unperturbed clock is highly optimized for robust rhythms, as very few gene perturbations are capable of simultaneously increasing amplitude and lowering stochastic noise. Ultimately, this study demonstrates the importance of considering the effect of circadian perturbations on stochastic noise, particularly with regard to the development of small-molecule circadian therapeutics.« less

  9. Algae culture for cattle feed and water purification. Final report

    SciTech Connect (OSTI)

    Varani, F.T.; Schellenbach, S.; Veatch, M.; Grover, P.; Benemann, J.

    1980-05-16

    The feasibility of algae growth on centrate from anaerobic digester effluent and the refeed of both effluent solids and the algae to feedlot cattle were investigated. The digester was operated with dirt feedlot manure. The study serves as a supplement for the work to design a utility sized digester for the City of Lamar to convert local feedlot manure into a fuel gas. The biogas produced would power the electrical generation plant already in service. Previous studies have established techniques of digester operation and the nutritional value for effluent solids as fed to cattle. The inclusion of a single-strain of algae, Chlorella pyrenidosa in the process was evaluated here for its capability (1) to be grown in both open and closed ponds of the discharge water from the solids separation part of the process, (2) to purify the discharge water, and (3) to act as a growth stimulant for cattle feed consumption and conversion when fed at a rate of 6 grams per head per day. Although it was found that the algae could be cultured and grown on the discharge water in the laboratory, the study was unable to show that algae could accomplish the other objectives successfully. However, the study yielded supplementary information useful to the overall process design of the utility plant. This was (1) measurement of undried digester solids fed to cattle in a silage finishing ration (without algae) at an economic value of $74.99 per dry ton based on nutritional qualities, (2) development of a centrate treatment system to decolorize and disinfect centrate to allow optimum algae growth, and (3) information on ionic and mass balances for the digestion system. It is the recommendation of this study that algae not be used in the process in the Lamar bioconversion plant.

  10. Environmental resources of selected areas of Hawaii: Cultural environment and aesthetic resources

    SciTech Connect (OSTI)

    Trettin, L.D.; Petrich, C.H.; Saulsbury, J.W.

    1996-01-01

    This report has been prepared to make available and archive the background scientific data and related information collected on the cultural environment and aesthetic resources during the preparation of the environmental impact statement (EIS) for Phases 3 and 4 of the Hawaii Geothermal Project (HGP) as defined by the state of Hawaii in its April 1989 proposal to Congress. The cultural environment in the Geothermal Resource Zone (GRZ) and associated study area consists of Native Hawaiian cultural and religious practices and both Native Hawaiian and non-Native Hawaiian cultural resources. This report consists of three sections: (1) a description of Native Hawaiian cultural and religious rights, practices, and values; (2) a description of historic, prehistoric, and traditional Native Hawaiian sites; and (3) a description of other (non-native) sites that could be affected by development in the study area. Within each section, the level of descriptive detail varies according to the information currently available. The description of the cultural environment is most specific in its coverage of the Geothermal Resource Subzones in the Puna District of the island of Hawaii and the study area of South Maui. Ethnographic and archaeological reports by Cultural Advocacy Network Developing Options and International Archaeological Research Institute, Inc., respectively, supplement the descriptions of these two areas with new information collected specifically for this study. Less detailed descriptions of additional study areas on Oahu, Maui, Molokai, and the island of Hawaii are based on existing archaeological surveys.

  11. Cultural Resource Protection Plan for the Remote-Handled Low-Level Waste Disposal Facility at the Idaho National Laboratory

    SciTech Connect (OSTI)

    Pace, Brenda Ringe; Gilbert, Hollie Kae

    2015-05-01

    This plan addresses cultural resource protection procedures to be implemented during construction of the Remote Handled Low Level Waste project at the Idaho National Laboratory. The plan proposes pre-construction review of proposed ground disturbing activities to confirm avoidance of cultural resources. Depending on the final project footprint, cultural resource protection strategies might also include additional survey, protective fencing, cultural resource mapping and relocation of surface artifacts, collection of surface artifacts for permanent curation, confirmation of undisturbed historic canal segments outside the area of potential effects for construction, and/or archaeological test excavations to assess potential subsurface cultural deposits at known cultural resource locations. Additionally, all initial ground disturbing activities will be monitored for subsurface cultural resource finds, cultural resource sensitivity training will be conducted for all construction field personnel, and a stop work procedure will be implemented to guide assessment and protection of any unanticipated discoveries after initial monitoring of ground disturbance.

  12. EIS-0238: Withdrawal of Notice of Intent to Prepare an Environmental...

    Broader source: Energy.gov (indexed) [DOE]

    Proposed Minnesota Agri-Power Plant and Associated Facilities On October 7, 1998 (63 FR ... facility, known as the Minnesota Agri- Power Plant (MAPP), and associated transmission ...

  13. Stabilization of gene expression and cell morphology after explant recycling during fin explant culture in goldfish

    SciTech Connect (OSTI)

    Chenais, Nathalie; Lareyre, Jean-Jacques; Le Bail, Pierre-Yves; Labbe, Catherine

    2015-07-01

    The development of fin primary cell cultures for in vitro cellular and physiological studies is hampered by slow cell outgrowth, low proliferation rate, poor viability, and sparse cell characterization. Here, we investigated whether the recycling of fresh explants after a first conventional culture could improve physiological stability and sustainability of the culture. The recycled explants were able to give a supplementary cell culture showing faster outgrowth, cleaner cell layers and higher net cell production. The cells exhibited a highly stabilized profile for marker gene expression including a low cytokeratin 49 (epithelial marker) and a high collagen 1a1 (mesenchymal marker) expression. Added to the cell spindle-shaped morphology, motility behavior, and actin organization, this suggests that the cells bore stable mesenchymal characteristics. This contrast with the time-evolving expression pattern observed in the control fresh explants during the first 2 weeks of culture: a sharp decrease in cytokeratin 49 expression was concomitant with a gradual increase in col1a1. We surmise that such loss of epithelial features for the benefit of mesenchymal ones was triggered by an epithelial to mesenchymal transition (EMT) process or by way of a progressive population replacement process. Overall, our findings provide a comprehensive characterization of this new primary culture model bearing mesenchymal features and whose stability over culture time makes those cells good candidates for cell reprogramming prior to nuclear transfer, in a context of fish genome preservation. - Highlights: • Recycled fin explants outgrow cells bearing stable mesenchymal traits. • Cell production and quality is enhanced in the recycled explant culture system. • Fresh fin primary culture is highly variable and loose epithelial traits over time.

  14. Safety Reports Series No. 11, Developing Safety Culture in Nuclear Activities: Practical Suggestions to Assist Progress, International Atomic Energy Agency

    Broader source: Energy.gov [DOE]

    Safety Reports Series No. 11, Developing Safety Culture in Nuclear Activities: Practical Suggestions to Assist Progress, International Atomic Energy Agency

  15. Environmental guidelines for Development of Cultural Resource Management plans. Working draft for comment

    SciTech Connect (OSTI)

    Not Available

    1994-04-01

    DOE has stewardship responsibilities for managing the cultural resources remaining on DOE-owned and other lands impacted by DOE programs. Goal of the DOE-wide Cultural Resource Management (CRM) program is to identify and consolidate compliance actions associated with statutory and regulatory requirements. This document is to provide guidelines to DOE field managers; its implementation is intended to assure that each DOE facility and program complies with executive orders, statutes, and regulations governing the management of cultural resources. It covers CRM goals, existing conditions, CRM methods, CRM procedures and administration, and plan attachments. Glossary, legislation, and documents are covered in appendices.

  16. Environmental guidelines for development of Cultural Resource Management plans. Final report

    SciTech Connect (OSTI)

    1995-08-01

    The purpose of this document is to provide guidelines to the DOE field managements with responsibility for the development of an individual Cultural Resource Management Plan for each DOE facility and program.

  17. Proceedings of the Advisory Committee on Reactor Safeguards Safety Culture Workshop

    Office of Energy Efficiency and Renewable Energy (EERE)

    NUREG/CP-0183, Proceedings of the Advisory Committee on Reactor Safeguards Safety Culture Workshop, June 12, 2003 U.S. Nuclear Regulatory Commission Advisory Committee on Reactor Safeguards Washington, DC 20555-0001.

  18. Reconstitution activity of hypoxic cultured human cord blood CD34-positive cells in NOG mice

    SciTech Connect (OSTI)

    Shima, Haruko; Takubo, Keiyo; Iwasaki, Hiroko; Yoshihara, Hiroki; Gomei, Yumiko; Hosokawa, Kentaro; Arai, Fumio; Takahashi, Takao; Suda, Toshio

    2009-01-16

    Hematopoietic stem cells (HSCs) reside in hypoxic areas of the bone marrow. However, the role of hypoxia in the maintenance of HSCs has not been fully characterized. We performed xenotransplantation of human cord blood cells cultured in hypoxic or normoxic conditions into adult NOD/SCID/IL-2R{gamma}{sup null} (NOG) mice. Hypoxic culture (1% O{sub 2}) for 6 days efficiently supported the maintenance of HSCs, although cell proliferation was suppressed compared to the normoxic culture. In contrast, hypoxia did not affect in vitro colony-forming ability. Upregulation of a cell cycle inhibitor, p21, was observed in hypoxic culture. Immunohistochemical analysis of recipient bone marrow revealed that engrafted CD34{sup +}CD38{sup -} cord blood HSCs were hypoxic. Taken together, these results demonstrate the significance of hypoxia in the maintenance of quiescent human cord blood HSCs.

  19. DOE P 141.1 Department of Energy Management of Cultural Resources

    Energy Savers [EERE]

    U.S. Department of Energy POLICY Washington, D.C. Approved: 5-2-01 SUBJECT: DEPARTMENT OF ENERGY MANAGEMENT OF CULTURAL RESOURCES PURPOSE AND SCOPE The purpose of this Policy is- *...

  20. Enhanced growth medium and method for culturing human mammary epithelial cells

    DOE Patents [OSTI]

    Stampfer, Martha R.; Smith, Helene S.; Hackett, Adeline J.

    1983-01-01

    Methods are disclosed for isolating and culturing human mammary epithelial cells of both normal and malignant origin. Tissue samples are digested with a mixture including the enzymes collagenase and hyaluronidase to produce clumps of cells substantially free from stroma and other undesired cellular material. Growing the clumps of cells in mass culture in an enriched medium containing particular growth factors allows for active cell proliferation and subculture. Clonal culture having plating efficiencies of up to 40% or greater may be obtained using individual cells derived from the mass culture by plating the cells on appropriate substrates in the enriched media. The clonal growth of cells so obtained is suitable for a quantitative assessment of the cytotoxicity of particular treatment. An exemplary assay for assessing the cytotoxicity of the drug adriamycin is presented.

  1. Pueblo De San Ildefonso Department of Environmental and Cultural Preservation Program Overview 2015

    Broader source: Energy.gov [DOE]

    At the September 30, 2015 meeting: Director Raymond Martinez, and Governor Mountain, provided the membership with an update on the Pueblo de San Ildefonso's perspectives on: environmental clean up and cultural preservation.

  2. Characteristics of enriched cultures for bio-huff-`n`-puff tests at Jilin oil field

    SciTech Connect (OSTI)

    Xiu-Yuan Wang; Gang Dai; Yan-Fen Xue; Shu-Hua Xie

    1995-12-31

    Three enriched cultures (48, 15a, and 26a), selected from more than 80 soil and water samples, could grow anaerobically in the presence of crude oil at 30{degrees}C and could ferment molasses to gases and organic acids. Oil recovery by culture 48 in the laboratory model experiment was enhanced by 25.2% over the original reserves and by 53.7% over the residual reserves. Enriched culture 48 was composed of at least 4 species belonging to the genera Eubacterium, Fusobacterium, and Bacteroides. This enriched culture was used as inoculum for MEOR field trials at Jilin oil field with satisfactory results. The importance of the role of these isolates in EOR was confirmed by their presence and behavior in the fluids produced from the microbiologically treated reservoir.

  3. Hanford Site, Tribes Raise Awareness of Culturally Significant Resources With Training Site

    Broader source: Energy.gov [DOE]

    RICHLAND, Wash. – About seven acres in size, the cultural resources test beds site is a small area of the 586-square-mile Hanford site. But its impact is big.

  4. EM’s Safety Chief Talks Safety Culture Improvements With EM Update

    Office of Energy Efficiency and Renewable Energy (EERE)

    WASHINGTON, D.C. – EM Safety, Security, and Quality Programs Deputy Assistant Secretary James Hutton recently spoke with EM Update about the EM program’s improvements in safety culture.

  5. DOE P 141.1 – Department of Energy Management of Cultural Resources

    Broader source: Energy.gov [DOE]

    The purpose of this Policy is (1) to ensure that U.S. Department of Energy (DOE) programs, including the National Nuclear Security Administration (NNSA), and field elements integrate cultural resources management into their missions and activities, and (2) to raise the level of awareness and accountability among DOE (including NNSA) contractors concerning the importance of DOE's cultural resource-related legal and trust responsibilities.

  6. DOE P 141.1 – Department of Energy Management of Cultural Resources (DOE, 2001)

    Broader source: Energy.gov [DOE]

    The purpose of this Policy is (1) to ensure that U.S. Department of Energy (DOE) programs, including the National Nuclear Security Administration (NNSA), and field elements integrate cultural resources management into their missions and activities, and (2) to raise the level of awareness and accountability among DOE (including NNSA) contractors concerning the importance of DOE's cultural resource-related legal and trust responsibilities.

  7. Idaho National Laboratory Cultural Resource Management Office FY 2010 Activity Report

    SciTech Connect (OSTI)

    Hollie K. Gilbert; Clayton F. Marler; Christina L. Olson; Brenda R. Pace; Julie Braun Williams

    2011-09-01

    The Idaho National Laboratory (INL) Site is home to vast numbers and a wide variety of important cultural resources representing at least a 13,500 year span of human land use in the region. As a federal agency, the Department of Energy, Idaho Operations Office (DOE-ID) has legal responsibility for the management and protection of the resources and has contracted these responsibilities to Battelle Energy Alliance (BEA). The BEA professional staff is committed to maintaining a cultural resource management program that accepts the challenge of preserving INL cultural resources in a manner reflecting their importance in local, regional, and national history. This report summarizes activities performed by the INL Cultural Resource Management Office (CRMO) staff during fiscal year 2010. This work is diverse, far-reaching and though generally confined to INL cultural resource compliance, also includes a myriad of professional and voluntary community activities. This document is intended to be informative to both internal and external stakeholders and to serve as a planning tool for future INL cultural resource management work.

  8. Idaho National Laboratory Cultural Resource Management Office FY 2011 Activity Report

    SciTech Connect (OSTI)

    Julie Braun Williams; Brenda R. Pace; Hollie K. Gilbert; Christina L. Olson

    2012-09-01

    The Idaho National Laboratory (INL) Site is home to vast numbers and a wide variety of important cultural resources representing at least a 13,500 year span of human land use in the region. As a federal agency, the Department of Energy, Idaho Operations Office (DOE-ID) has legal responsibility for the management and protection of the resources and has contracted these responsibilities to Battelle Energy Alliance (BEA). The BEA professional staff is committed to maintaining a cultural resource management program that accepts the challenge of preserving INL cultural resources in a manner reflecting their importance in local, regional, and national history. This report is intended as a stand-alone document that summarizes activities performed by the INL Cultural Resource Management Office (CRMO) staff during fiscal year 2011. This work is diverse, far-reaching and though generally confined to INL cultural resource compliance, also includes a myriad of professional and voluntary community activities. This document is intended to be informative to both internal and external stakeholders, serve as a planning tool for future INL cultural resource management work, and meet an agreed upon legal requirement.

  9. Relationship between substrate concentration and fermentation product ratios in Clostridium thermocellum cultures

    SciTech Connect (OSTI)

    Brener, D.; Johnson, B.F.

    1984-05-01

    Growth of Clostridium thermocellum in batch cultures was studied over a broad range of cellobiose concentrations. Cultures displayed important differences in their substrate metabolism as determined by the end product yields. Bacterial growth was severly limited when the initial cellobiose concentration was 0.2 (wt/vol), was maximal at substrate concentrations between 0.5 and 2.0%, and did not occur at 5.0% cellobiose. Ethanol accumulated maximally (38.3% ..mu..mol/10/sup 9/ cells) in cultures with an initial cellobiose concentration of 0.8%, whereas cultures in 2.0% cellobiose accumulated on 17.3 ..mu..mol, and substrate-limited cultures (0.2% cellobiose) accumulated little, if any, ethanol beyond that initially detected (8.3 ..mu..mol/10/sup 9/ cells). In a medium with 0.8% cellobiose, ethanol was produced at a constant rate of approximately 1.1 ..mu..mol/10/sup 9/ cells per h from late-logarithmic phase (16 h) of growth well into stationary phase (44 h). When ethanol was added exogenously at levels more than twice the maximum produced by the cultures themselves (0.5% vol/vol), neither the extent of growth (maximum Klett units, 150) nor the amounts of ethanol produced (approximately 0.17%) by the culture was affected. The ratio of ethanol to acetate was highest (2.8) when cells were grown in 0.8% cellobiose and lowest (1.2) when cells were grown in 0.2% cellobiose. 18 references.

  10. Development of a cell culture surface conversion technique using alginate thin film for evaluating effect upon cellular differentiation

    SciTech Connect (OSTI)

    Nakashima, Y.; Tsusu, K.; Minami, K.; Nakanishi, Y.

    2014-06-15

    Here, we sought to develop a cell culture surface conversion technique that would not damage living cells. An alginate thin film, formed on a glass plate by spin coating of sodium alginate solution and dipping into calcium chloride solution, was used to inhibit adhesion of cells. The film could be removed by ethylenediaminetetraacetate (EDTA) at any time during cell culture, permitting observation of cellular responses to conversion of the culture surface in real time. Additionally, we demonstrated the validity of the alginate thin film coating method and the performance of the film. The thickness of the alginate thin film was controlled by varying the rotation speed during spin coating. Moreover, the alginate thin film completely inhibited the adhesion of cultured cells to the culture surface, irrespective of the thickness of the film. When the alginate thin film was removed from the culture surface by EDTA, the cultured cells adhered to the culture surface, and their morphology changed. Finally, we achieved effective differentiation of C2C12 myoblasts into myotube cells by cell culture on the convertible culture surface, demonstrating the utility of our novel technique.

  11. Solutions from the Land: Developing a New Vision for United States Agriculture, Forestry, and Conservation

    Broader source: Energy.gov [DOE]

    Opening Plenary Session: Bioenergy Sustainability—Charting the Path toward a Viable Future Nathan Rudgers, Senior Vice President, Farm Credit East

  12. Environmental and economic evaluation of energy recovery from agricultural and forestry residues

    SciTech Connect (OSTI)

    1980-09-01

    Four conversion methods and five residues are examined in this report, which describes six model systems: hydrolysis of corn residues, pyrolysis of corn residues, combustion of cotton-ginning residues, pyrolysis of wheat residues, fermentation of molasses, and combustion of pulp and papermill wastes. Estimates of material and energy flows for those systems are given per 10/sup 12/ Btu of recovered energy. Regional effects are incorporated by addressing the regionalized production of the residues. A national scope cannot be provided for every residue considered because of the biological and physical constraints of crop production. Thus, regionalization of the model systems to the primary production region for the crop from which the residue is obtained has been undertaken. The associated environmental consequences of residue utilization are then assessed for the production region. In addition, the environmental impacts of operating the model systems are examined by quantifying the residuals generated and the land, water, and material requirements per 10/sup 12/ Btu of energy generated. On the basis of estimates found in the literature, capital, operating, and maintenance cost estimates are given for the model systems. These data are also computed on the basis of 10/sup 12/ Btu of energy recovered. The cost, residual, material, land, and water data were then organized into a format acceptable for input into the SEAS data management program. The study indicates that the most serious environmental impacts arise from residue removal rather than from conversion.

  13. Effects of propionate and acetate additions on solvent production in batch cultures of clostridium acetobutylicum

    SciTech Connect (OSTI)

    Huesemann, M.H.W. ); Papoutsakis, E.T. )

    1990-05-01

    Addition of acetate or propionate to uncontrolled-pH batch cultures does not affect the initiation of solventogenesis but does enhance final solvent concentrations compared with those of unchallenged cultures. This observation can be explained in terms of the increased buffering capacity of the medium brought about by the added acids, resulting in protection against premature growth inhibition due to low culture pH values at the end of the fermentation. The uptake of propionic acid from the medium does not proceed solely via the coenzyme A-transferase pathway, since less acetone than propanol is formed. Therefore, at least 50% of the propionic acid is taken up through the reversed kinase-phosphotransbutyrylase reaction pathway.

  14. Concentration-dependent gene expression responses to flusilazole in embryonic stem cell differentiation cultures

    SciTech Connect (OSTI)

    Dartel, Dorien A.M. van; Pennings, Jeroen L.A.; Fonteyne, Liset J.J. de la; Brauers, Karen J.J.; Claessen, Sandra; Delft, Joost H. van; Kleinjans, Jos C.S.; Piersma, Aldert H.

    2011-03-01

    The murine embryonic stem cell test (EST) is designed to evaluate developmental toxicity based on compound-induced inhibition of embryonic stem cell (ESC) differentiation into cardiomyocytes. The addition of transcriptomic evaluation within the EST may result in enhanced predictability and improved characterization of the applicability domain, therefore improving usage of the EST for regulatory testing strategies. Transcriptomic analyses assessing factors critical for risk assessment (i.e. dose) are needed to determine the value of transcriptomic evaluation in the EST. Here, using the developmentally toxic compound, flusilazole, we investigated the effect of compound concentration on gene expression regulation and toxicity prediction in ESC differentiation cultures. Cultures were exposed for 24 h to multiple concentrations of flusilazole (0.54-54 {mu}M) and RNA was isolated. In addition, we sampled control cultures 0, 24, and 48 h to evaluate the transcriptomic status of the cultures across differentiation. Transcriptomic profiling identified a higher sensitivity of development-related processes as compared to cell division-related processes in flusilazole-exposed differentiation cultures. Furthermore, the sterol synthesis-related mode of action of flusilazole toxicity was detected. Principal component analysis using gene sets related to normal ESC differentiation was used to describe the dynamics of ESC differentiation, defined as the 'differentiation track'. The concentration-dependent effects on development were reflected in the significance of deviation of flusilazole-exposed cultures from this transcriptomic-based differentiation track. Thus, the detection of developmental toxicity in EST using transcriptomics was shown to be compound concentration-dependent. This study provides further insight into the possible application of transcriptomics in the EST as an improved alternative model system for developmental toxicity testing.

  15. Co-culture of 3D tumor spheroids with fibroblasts as a model for epithelial–mesenchymal transition in vitro

    SciTech Connect (OSTI)

    Kim, Sun-Ah; Lee, Eun Kyung; Kuh, Hyo-Jeong

    2015-07-15

    Epithelial–mesenchymal transition (EMT) acts as a facilitator of metastatic dissemination in the invasive margin of malignant tumors where active tumor–stromal crosstalks take place. Co-cultures of cancer cells with cancer-associated fibroblasts (CAFs) are often used as in vitro models of EMT. We established a tumor–fibroblast proximity co-culture using HT-29 tumor spheroids (TSs) with CCD-18co fibroblasts. When co-cultured with TSs, CCD-18co appeared activated, and proliferative activity as well as cell migration increased. Expression of fibronectin increased whereas laminin and type I collagen decreased in TSs co-cultured with fibroblasts compared to TSs alone, closely resembling the margin of in vivo xenograft tissue. Active TGFβ1 in culture media significantly increased in TS co-cultures but not in 2D co-cultures of cancer cells–fibroblasts, indicating that 3D context-associated factors from TSs may be crucial to crosstalks between cancer cells and fibroblasts. We also observed in TSs co-cultured with fibroblasts increased expression of α-SMA, EGFR and CTGF; reduced expression of membranous β-catenin and E-cadherin, together suggesting an EMT-like changes similar to a marginal region of xenograft tissue in vivo. Overall, our in vitro TS–fibroblast proximity co-culture mimics the EMT-state of the invasive margin of in vivo tumors in early metastasis. - Highlights: • An adjacent co-culture of tumor spheroids and fibroblasts is presented as EMT model. • Activation of fibroblasts and increased cell migration were shown in co-culture. • Expression of EMT-related factors in co-culture was similar to that in tumor tissue. • Crosstalk between spheroids and fibroblasts was demonstrated by secretome analysis.

  16. Guidelines for Evaluating and Documenting Traditional Cultural Properties (NPS, 1990, revised 1998)

    Broader source: Energy.gov [DOE]

    This National Park Service bulletin is meant to assist federal agencies, State Historic Preservation Officers, local governments, Indian tribes, and other historic preservation practitioners in determining whether properties thought to have traditional cultural significance are eligible for inclusion in the National Register of Historic Places.

  17. LIPID PRODUCTION BY DUNALIELLA SALINA IN BATCH CULTURE: EFFECTS OF NITROGEN LIMITATION AND LIGHT INTENSITY

    SciTech Connect (OSTI)

    Weldy, C.S.; Huesemann, M.

    2007-01-01

    Atmospheric carbon dioxide (CO2) concentrations are increasing and may cause unknown deleterious environmental effects if left unchecked. The Intergovernmental Panel on Climate Change (IPCC) has predicted in its latest report a 2C to 4C increase in global temperatures even with the strictest CO2 mitigation practices. Global warming can be attributed in large part to the burning of carbon-based fossil fuels, as the concentration of atmospheric CO2 is directly related to the burning of fossil fuels. Biofuels which do not add CO2 to the atmosphere are presently generated primarily from terrestrial plants, i.e., ethanol from corn grain and biodiesel from soybean oil. The production of biofuels from terrestrial plants is severely limited by the availability of fertile land. Lipid production from microalgae and its corresponding biodiesel production have been studied since the late 1970s but large scale production has remained economically infeasible due to the large costs of sterile growing conditions required for many algal species. This study focuses on the potential of the halophilic microalgae species Dunaliella salina as a source of lipids and subsequent biodiesel production. The lipid production rates under high light and low light as well as nitrogen suffi cient and nitrogen defi cient culture conditions were compared for D. salina cultured in replicate photobioreactors. The results show (a) cellular lipid content ranging from 16 to 44% (wt), (b) a maximum culture lipid concentration of 450mg lipid/L, and (c) a maximum integrated lipid production rate of 46mg lipid/L culture*day. The high amount of lipids produced suggests that D. salina, which can be mass-cultured in non-sterile outdoor ponds, has strong potential to be an economically valuable source for renewable oil and biodiesel production.

  18. U.S. assistance enhancing safety culture in countries operating Soviet-designed reactors

    SciTech Connect (OSTI)

    Guppy, J.G.; Horak, W.C.; Reisman, A.W.

    1995-05-01

    The United States Department of Energy (USDOE) is managing the International Nuclear Safety Program (INSP), which is aimed at providing assistance to enhance safety at commercial nuclear power plants (NPPS) in Russia and Ukraine, as well as Central European countries (CEC). The funding for this program has been provided by the US Agency for International Development (AID). Brookhaven National Laboratory has been assisting DOE in certain portions of this program. The enhancement of safety culture is one of the most important goals of the joint International Nuclear Safety Program. In terms of the INSP, safety culture is comprised of two major components; (1) an environment that is a function of regulations, management sensitivity and structure; and (2) an individual commitment to safety in the day to day execution of activities in terms of thought and accountability. The long term impact of the INSP activities can only be measured by the effectiveness of strengthening safety culture within our partner counties. The strengthening of this culture will manifest in reduced risk of a nuclear accident long after other evidence of the INSP activities has disappeared. One area within the INSP, which has already led to a number of successful specific projects, is under the plant safety upgrade activities. Here, the US and the partner countries jointly identify specific target areas for the INSP efforts. Each identified area has a major component involving safety culture enhancement. With any direct involvement in the particular assistance activities, areas are identified to include a need for training. As technical experts and management from the partner country are assisted in addressing the identified needs, the training programs are provided which will not only address the specific need at hand, but will also teach skills which can be applied to different, but related needs that may exist or develop.

  19. Tombs, tunnels, and terraces a cultural resources survey of a former ammunition supply point in Okinawa, Japan

    SciTech Connect (OSTI)

    Verhaaren, B. T.; Levenson, J. B.; Komine, G.

    2000-02-09

    U.S. forces serving at military bases on foreign soil are obligated to act as good stewards of the cultural and natural resources under their control. However, cultural resources management presents special challenges at U.S. bases in other countries where cultural properties laws differ in emphasis and detail from those in the United States and issues of land ownership and occupancy are not always clear. Where status of forces agreements (SOFAs) exist, environmental governing standards bridge the gap between U.S. and host nation cultural priorities. In Japan, the Department of Defense Japan Environmental Governing Standards (JEGS) fill this function. Under Criteria 12-4.2 and 12-4.3 of the JEGS, U.S. Forces Japan commit themselves to inventory and protect cultural properties found on the lands they control or use. Cultural properties include archaeological sites, tombs, historic buildings, and shrines. Natural monuments, such as landscape features or plant and animal species, may also be designated as cultural properties. As part of this commitment, in February 1999 a cultural resources inventory was conducted in Area 1, part of Kadena Air Base (AB), Okinawa, Japan. Area 1, the former U.S. army Ammunition Supply Point 1, is currently used primarily for training exercises and recreational paint ball.

  20. A chemically defined culture medium containing Rho kinase inhibitor Y-27632 for the fabrication of stratified squamous epithelial cell grafts

    SciTech Connect (OSTI)

    Aslanova, Afag; Takagi, Ryo; Yamato, Masayuki; Okano, Teruo; Yamamoto, Masakazu

    2015-05-01

    With the development of a culture method for stratified squamous epithelial cells, tissue-engineered epithelial cell sheets have been successfully applied as clinical cell grafts. However, the implementation of these cell sheets without the use of any animal-derived materials is highly desirable. In this study, Rho-associated protein kinase inhibitor Y-27632 was used to develop a chemically defined culture medium for the fabrication of stratified epithelial cell grafts consisting of human epidermal and oral keratinocytes, and the proliferation activity, cell morphology, and gene expressions of the keratinocytes were analyzed. The results of a colorimetric assay indicated that Y-27632 significantly promoted the proliferation of the keratinocytes in culture media both with and without fetal bovine serum (FBS), although there were no indications of Y-27632 efficacy on cell morphology and stratification of the keratinocytes in culture medium without any animal-derived materials. The results of quantitative RT-PCR revealed that gene expressions correlated with cell adhesion, cell–cell junction, proliferation markers, and stem/progenitor markers in cultured keratinocytes were not strongly affected by the addition of Y-27632 to the culture medium. Moreover, gene expressions of differentiation markers in stratified keratinocytes cultured in medium without FBS were nearly identical to those of keratinocytes co-cultured with 3T3 feeder cells. Interestingly, the expressions of differentiation markers in cultured stratified keratinocytes were suppressed by FBS, whereas they were reconstructed by either co-culture of a 3T3 feeder layer or addition of Y-27632 into the culture medium containing FBS. These findings indicate that Y-27632 is a useful supplement for the development of a chemically defined culture medium for fabrication of stratified epithelial cell grafts for clinical applications for the purpose of developing the culture medium with a lower risk of pathogen

  1. Comparison of Marine Microalgae Culture Systems for Fuels Production and Carbon Sequestration

    SciTech Connect (OSTI)

    Weissman, Joseph C; Polle, Juergen

    2006-05-30

    The dual problems of global fossil fuels supplies and global warming focus attention on the need to develop technologies that can provide large amounts of renewable fuels without contributing to global warming. The capture of power plant flue gas CO2 using microalgae cultures is one potential technology that could meet this objective. The central R&D issues are the design and operation of low-cost algal mass culture systems and the development of algal strains and cultivation techniques that can achieve very high biomass productivities. The major objective of this project was to develop mass culture techniques that could result in greatly increased biomass productivities, well above the about 50 metric tons per hectare per year (mt/ha/y) currently achievable. In this project, two marine microalgae species, the diatom Cyclotella sp.. and the green alga Tetraselmis sp., were cultivated on seawater in both open ponds and closed photo bioreactors, under a variety of different cultivation conditions. Simultaneous operation of the closed photo bioreactors and open ponds demonstrated similar productivities, under the same operating conditions. Thus the very expensive closed systems do not provide any major or inherent advantages in microalgae production over open ponds. Mutants of Cyclotella sp. were developed that exhibited reduced pigment content, which theoretically would result in greatly increased productivities when grown under full sunlight. However, in open ponds, these mutant strains exhibited similar productivities as the parental strains. The mutant strains all grew relatively slowly, suggesting that additional mutations masked whatever inherent potential for increased productivities may have resulted from the reduced pigment content. Research is still required to develop improved low pigment strains. When open pond cultures were exposed to intermittent sunlight, by partially covering the ponds with slats, solar conversion efficiencies increased dramatically

  2. Quality of Cultural Heritage in EIA; twenty years of experience in Norway

    SciTech Connect (OSTI)

    Lindblom, Inge

    2012-04-15

    The aim of this paper is to clarify and discuss how quality, relevance, attitudes, beliefs and transfer value act as underlying driving forces in the development of the Cultural Heritage theme in EIAs. One purpose is to identify and discuss some conditions that can better environmental assessment in order to increase the significance of EIA in decision-making with regard to Cultural Heritage. The main tools used are different research methods designed for analyses of quality and quality changes, primarily based on the relevant opinions of 160 people occupied with Cultural Heritage in EIA in Norway. The study is based on a review of 40 types of EIAs from 1991 to 2000, an online questionnaire to 319 (160 responded) individuals from 14 different backgrounds, and interviews with three institutions in Sweden and Denmark. The study confirms a steadily increasing quality on EIRs over time, parallel with an improvement of the way in which Cultural Heritage is treated in EIA. This is supported by both the interviews and the qualitative comments regarding the survey. Potential for improvements is shown to be a need for more detailed background material as well as more use of adequate methods. The survey shows the existence of a wide variety of negative views, attitudes and beliefs, but the consequences of this are difficult to evaluate. However, most certainly, negative attitudes and beliefs have not been powerful enough to be detrimental to the quality of Cultural Heritage component, as nothing in the study indicates that negative attitudes and myths are undermining the system of EIA. The study shows the importance of having on-going discussions on quality and quality change over time by people involved in EIA, and how this is a necessary condition for successful implementation and acceptance. Beliefs and negative attitudes can also be a catalyst for developing better practice and advancing new methodology. In addition, new EIA countries must be prepared for several years

  3. Cultural Resources

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Expand Projects & Initiatives Finance & Rates Expand Finance & Rates Involvement & Outreach Expand Involvement & Outreach Doing Business Expand Doing Business...

  4. Cultural Preservation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Counterterrorism NNSA provides expertise, practical tools, and technically informed policy recommendations required to advance U.S. nuclear counterterrorism and counterproliferation objectives. It executes a unique program of work focused solely on these missions and builds partnerships with U.S. government agencies and key foreign governments on these issues. Learn More Counterterrorism Policy and Cooperation Nuclear Threat Science Learn More About Counterterrorism Related News A snapshot of

  5. Open-access databases as unprecedented resources and drivers of cultural change in fisheries science

    SciTech Connect (OSTI)

    McManamay, Ryan A; Utz, Ryan

    2014-01-01

    Open-access databases with utility in fisheries science have grown exponentially in quantity and scope over the past decade, with profound impacts to our discipline. The management, distillation, and sharing of an exponentially growing stream of open-access data represents several fundamental challenges in fisheries science. Many of the currently available open-access resources may not be universally known among fisheries scientists. We therefore introduce many national- and global-scale open-access databases with applications in fisheries science and provide an example of how they can be harnessed to perform valuable analyses without additional field efforts. We also discuss how the development, maintenance, and utilization of open-access data are likely to pose technical, financial, and educational challenges to fisheries scientists. Such cultural implications that will coincide with the rapidly increasing availability of free data should compel the American Fisheries Society to actively address these problems now to help ease the forthcoming cultural transition.

  6. Self-Reliability and Motivation in a Nuclear Security Culture Enhancement Program

    SciTech Connect (OSTI)

    Rogers,E.; deBoer,G.; Crawford, C.; De Castro, K.; Landers, J.

    2009-10-19

    The threat of nuclear terrorism has become a global concern. Many countries continue to make efforts to strengthen nuclear security by enhancing systems of nuclear material protection, control, and accounting (MPC&A). Though MPC&A systems can significantly upgrade nuclear security, they do not eliminate the "human factor." Gen. Eugene Habiger, a former "Assistant Secretary for Safeguards and Security" at the U.S. Department of Energy’s (DOE) nuclear-weapons complex and a former commander of U.S. strategic nuclear forces, has observed that "good security is 20% equipment and 80% people." Although eliminating the "human factor" is not possible, accounting for and mitigating the risk of the insider threat is an essential element in establishing an effective nuclear security culture. This paper will consider the organizational role in mitigating the risk associated with the malicious insider through monitoring and enhancing human reliability and motivation as well as enhancing the nuclear security culture.

  7. Culturally relevant science: An approach to math science education for hispanics. Final technical report

    SciTech Connect (OSTI)

    Montellano, B.O. de

    1996-11-14

    This progress report summarizes results of a teacher workshop. A letter sent to 17 teachers who had participated in the workshop requested a report of any activities undertaken and copies of lesson plans and materials developed. Only nine responses were received, and not all of them demonstrated a satisfactory level of activity. Teachers who submitted materials showing the most promise were invited to participate in the Summer Writing Workshop. A partial first draft of a companion volume for the teacher`s manual was written which provides a rationale for culturally relevant science and presents the cultural and scientific background needed. The outline of the book is presented in Appendix 1. Appendix 2 is a sample chapter from the book.

  8. Self-Reliability and Motivation in a Nuclear Security Culture Enhancement Program

    SciTech Connect (OSTI)

    Crawford, Cary E.; de Boer, Gloria; De Castro, Kara; Landers, John; Rogers, Erin

    2010-10-01

    The threat of nuclear terrorism has become a global concern. Many countries continue to make efforts to strengthen nuclear security by enhancing systems of nuclear material protection, control, and accounting (MPC&A). Though MPC&A systems can significantly upgrade nuclear security, they do not eliminate the human factor. Gen. Eugene Habiger, a former Assistant Secretary for Safeguards and Security at the U.S. Department of Energys (DOE) nuclear-weapons complex and a former commander of U.S. strategic nuclear forces, has observed that good security is 20% equipment and 80% people.1 Although eliminating the human factor is not possible, accounting for and mitigating the risk of the insider threat is an essential element in establishing an effective nuclear security culture. This paper will consider the organizational role in mitigating the risk associated with the malicious insider through monitoring and enhancing human reliability and motivation as well as enhancing the nuclear security culture.

  9. Independent Oversight Follow-up Assessment of Safety Culture at the Waste

    Office of Environmental Management (EM)

    - January 2012 | Department of Energy Waste Treatment and Immobilization Plant - January 2012 Independent Oversight Assessment, Waste Treatment and Immobilization Plant - January 2012 January 2012 Assessment of the Nuclear Safety Culture and Management of Nuclear Safety Concerns at the Hanford Site Waste Treatment and Immobilization Plant The U.S. Department of Energy (DOE) Office of Enforcement and Oversight (Independent Oversight), within the Office of Health, Safety and Security (HSS),

  10. Bile Culture and Susceptibility Testing of Malignant Biliary Obstruction via PTBD

    SciTech Connect (OSTI)

    Yu Haipeng; Guo Zhi Xing Wenge; Guo Xiuying; Liu Fang; Li Baoguo

    2012-10-15

    Purpose: To assess the information obtained by bile culture and susceptibility testing for malignant biliary obstruction by a retrospective one-center study. Methods: A total of 694 patients with malignant biliary obstruction received percutaneous transhepatic biliary drainage during the period July 2003 to September 2010, and subsequently, bile specimens were collected during the procedure. Among the 694 patients, 485 were men and 209 were women, ranging in age from 38 to 78 years (mean age 62 years). Results: A total of 42.9% patients had a positive bile culture (298 of 694). Further, 57 species of microorganisms and 342 strains were identified; gram-positive bacteria accounted for 50.9% (174 of 342) and gram-negative bacteria accounted for 41.5% (142 of 342) of these strains. No anaerobes were obtained by culture during this study. The most common microorganisms were Enterococcus faecalis (41 of 342, 11.9%), Escherichia coli (34 of 342, 9.9%), Klebsiella pneumoniae (28 of 342, 8.2%), Staphylococcus epidermidis (19 of 342, 5.5%), Enterococcus (18 of 342, 5.3%), and Enterobacter cloacae (16 of 342, 4.7%). The percentage of {beta}-lactamase-producing gram-positive bacteria was 27.6% (48 of 174), and the percentage of gram-negative bacteria was 19.7% (28 of 142). The percentage of enzyme-producing Escherichia coli was 61.7% (21 of 34). Conclusion: The bile cultures in malignant biliary obstruction are different from those in the Tokyo Guidelines and other benign biliary obstruction researches, which indicates that a different antibacterial therapy should be applied. Thus, knowledge of the antimicrobial susceptibility data could aid in the better use of antibiotics for the empirical therapy of biliary infection combined with malignant biliary obstruction.

  11. PROMOTING THE ECONOMY AND CULTURAL WELFARE OF NOGALES AND SANTA CRUZ COUNTY

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Kino Park Nogales, Arizona 85621 Phone Number: (520) 287-3685 Fax Number: (520) 287-3687 Email: info@thenogaleschamber.org Website: www.thenogaleschamber.org PROMOTING THE ECONOMY AND CULTURAL WELFARE OF NOGALES AND SANTA CRUZ COUNTY June 20 th , 2016 Christopher Lawrence United States Department of Energy 1000 Independence Avenue, SW Washington, DC 20585 Re: Nogales Interconnection Project Presidential Permit Application Dear Mr. Lawrence: We are writing this letter in urgency for the U.S.

  12. Fermentation of cellulosic substrates in batch and continuous culture by Clostridium thermocellum

    SciTech Connect (OSTI)

    Lynd, L.R.; Grethlein, H.E.; Wolkin, R.H. )

    1989-12-01

    Fermentation of dilute-acid-pretreated mixed hardwood and Avicel by Clostridium thermocellum was compared in batch and continuous cultures. Results indicate that fermentation parameters, with the exception of free cellulase activity, are essentially the same for pretreated mixed hardwood and Avicel under a variety of conditions. Hydrolysis yields obtained with C. thermocellum cellulase acting either in vitro or in vivo were comparable to those previously reported for Trichoderma reesei on the same substrates.

  13. Holding Mother Earth Sacred: Photo Journal Through an American Indian and Canadian Aboriginal Cultural Lens

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    HOLDING MOTHER EARTH SACRED Developing Energy Resources * Creating Sustainable Jobs * Honoring Indigenous Beliefs A Photo Journal Through an American Indian and Canadian Aboriginal Cultural Lens Vision Intention / Intersecting Points of Focus * To highlight the important role Native American and Aboriginal workers have in conventional energy production, sustainable energy development and green job creation on tribal lands in the US and Canada * To raise awareness about OH & S and

  14. Idaho National Laboratory Cultural Resource Monitoring Report for Fiscal Year 2007

    SciTech Connect (OSTI)

    Brenda R. Pace

    2007-10-01

    This report describes the cultural resource monitoring activities of the Idaho National Laboratory’s (INL) Cultural Resource Management (CRM) Office during fiscal year 2007 (FY 2007). In FY 2007, 40 localities were revisited: two locations of heightened Shoshone-Bannock tribal sensitivity, four caves, three butte/craters, twelve prehistoric archaeological sites, two historic stage stations, nine historic homesteads, a portion of Goodale’s Cutoff of the Oregon Trail, a portion of historic trail T-16, one World War II dump, four buildings from the World War II period, and Experimental Breeder Reactor –I, a modern scientific facility and National Historic Landmark. Several INL project areas were also monitored in FY 2007. This included direct observation of ground disturbing activities within the Power Burst Facility (PBF, now designated as the Critical Infrastructure Test Range Complex – CITRC), backfilling operations associated with backhoe trenches along the Big Lost River, and geophysical surveys designed to pinpoint subsurface unexploded ordnance in the vicinity of the Naval Ordnance Disposal Area. Surprise checks were also made to three ongoing INL projects to ensure compliance with INL CRM Office recommendations to avoid impacts to cultural resources. Although some impacts were documented, no significant adverse effects that would threaten the National Register eligibility of any resource were observed at any location.

  15. Dereplicating and spatial mapping of secondary metabolites from fungal cultures in situ

    SciTech Connect (OSTI)

    Kertesz, Vilmos; Van Berkel, Gary J.; Sica, Vincent P.; Raja, Huzefa A.; El-Elimat, Tamam; Oberlies, Nicholas H.; Pearce, Cedric J.

    2015-07-30

    Ambient ionization techniques coupled to mass spectrometry have recently become prevalent in natural product research due to their ability to examine secondary metabolites in situ. Identifying, mapping, and monitoring secondary metabolites directly on an organism provides invaluable spatial and temporal details that are lost through traditional extraction processes. Most ambient ionization techniques do not collect mutually supportive data, such as chromatographic retention times and/or UV/VIS spectra, and this can limit the ability to identify certain metabolites, such as differentiating isomers. To overcome this, the droplet liquid microjunction surface sampling probe (droplet LMJ SSP) was coupled with UPLC PDA HRMS MS/MS, thus providing separation, retention times, and UV/VIS data used in traditional dereplication protocols. By capturing these mutually supportive data, the identity of secondary metabolites could be confidently and rapidly assigned in situ. Using the droplet LMJ SSP, a protocol was constructed to analyze the secondary metabolite profile of fungal cultures directly without any sample preparation. The results demonstrate that fungal cultures can be dereplicated from the Petri dish, thus identifying secondary metabolites, including isomers, and confirming them against reference standards. As a result, heat maps, similar to mass spectrometry imaging, can be used to ascertain the location and relative concentration of secondary metabolites directly on the surface and/or surroundings of a fungal culture.

  16. Columbia River System Operation Review : Final Environmental Impact Statement, Appendix D: Cultural Resources.

    SciTech Connect (OSTI)

    Columbia River System Operation Review

    1995-11-01

    This study attempts to identify and analyze the impacts of the System Operating Strategy (SOS) alternatives on cultural resources. The impacts include effects on Native American traditional cultural values, properties and practices. They also include effects on archeological or historic properties meeting the criteria of the National Register of Historic Places. In addition to responding to the requirements of the National Environmental Policy Act (NEPA), this analysis addresses the requirements of the National Historic Preservation Act (NHPA), the Archeological Resources Protection Act (ARPA), the Native American Graves Protection and Repatriation Act (NAGPRA), the Native American Religious Freedom Act (NARFA), and other relevant legislation. To meet their legally mandated cultural resources requirements, the SOR agencies will develop agreements and Implementation Plans with the appropriate State Historic Preservation Officers (SHPOs), Tribes, and the Advisory Council on Historic Preservation (ACHP) detailing the measures necessary to best manage the resource. The planning and implementation activities will be staged over a number of years in consultation with affected Tribes.

  17. Research and development of shallow algal mass culture systems for the production of oils

    SciTech Connect (OSTI)

    Laws, E.A.

    1984-10-01

    The major accomplishment of the past nine months' work was the identification of a microalgal species which can be grown in the system on a 12-month basis without temperature control. The most promising species identified to date is a strain of platymonas sp. This strain grows rapidly at temperatures from 20/sup 0/ to 34/sup 0/C, and at salinities from 1.5 to 3.5%. Neither the lower temperature limit nor the lower salinity limit of the strain are known at this time. A factorial experiment designed to determine optimum growth conditions indicated that the optimum culture depth was 10 cm, the optimum pH about 7.5, and the optimum flow rate about 30 cm/s. A major discovery was that diluting the culture every third day greatly enhanced production. In this dilution mode daily yields averaged 46 g/m/sup 2/ ash-free dry weight (AFDW) over a one-month period, and photosynthetic efficiencies averaged 11% (based on visible light energy). The former figure is over twice the best long-term yields achieved in microalgal mass culture systems grown exclusively on inorganic nutrients.

  18. Ethanol-induced cell damage in cultured rat antral mucosa assessed by chromium-51 release

    SciTech Connect (OSTI)

    Sewell, R.B.; Ling, T.S.; Yeomans, N.D.

    1986-08-01

    We have developed an in vitro method for studying ethanol-induced injury to gastric mucosa using organ culture of rat antrum. Cell damage was assessed by measurement of the release of (/sup 51/Cr)sodium chromate from preloaded cells, a method adapted from a standard immunologic technique. This system provided rapid and highly reproducible quantitation of tissue injury as assessed by /sup 51/Cr release into the culture medium. The threshold concentration for ethanol-induced damage was between 10 and 15% v/v, similar to in vivo thresholds observed by others. /sup 51/Cr release could also be induced by very short exposure to ethanol (5-15 min), and then continued despite ethanol removal. Interestingly, after continuous ethanol exposure, a plateau of maximum /sup 51/Cr release was reached 60 min after exposure to ethanol over the concentration range 20-50%, suggesting tissue adaptation to ethanol damage. This organ culture system, which allows precise control of experimental conditions, may be useful for studying mechanisms of gastric mucosal injury and protection.

  19. Dereplicating and spatial mapping of secondary metabolites from fungal cultures in situ

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Kertesz, Vilmos; Van Berkel, Gary J.; Sica, Vincent P.; Raja, Huzefa A.; El-Elimat, Tamam; Oberlies, Nicholas H.; Pearce, Cedric J.

    2015-07-30

    Ambient ionization techniques coupled to mass spectrometry have recently become prevalent in natural product research due to their ability to examine secondary metabolites in situ. Identifying, mapping, and monitoring secondary metabolites directly on an organism provides invaluable spatial and temporal details that are lost through traditional extraction processes. Most ambient ionization techniques do not collect mutually supportive data, such as chromatographic retention times and/or UV/VIS spectra, and this can limit the ability to identify certain metabolites, such as differentiating isomers. To overcome this, the droplet liquid microjunction surface sampling probe (droplet LMJ SSP) was coupled with UPLC PDA HRMS MS/MS,more » thus providing separation, retention times, and UV/VIS data used in traditional dereplication protocols. By capturing these mutually supportive data, the identity of secondary metabolites could be confidently and rapidly assigned in situ. Using the droplet LMJ SSP, a protocol was constructed to analyze the secondary metabolite profile of fungal cultures directly without any sample preparation. The results demonstrate that fungal cultures can be dereplicated from the Petri dish, thus identifying secondary metabolites, including isomers, and confirming them against reference standards. As a result, heat maps, similar to mass spectrometry imaging, can be used to ascertain the location and relative concentration of secondary metabolites directly on the surface and/or surroundings of a fungal culture.« less

  20. On-Chip Dielectrophoretic Separation and Concentration of Viable, Non-Viable and Viable but Not Culturable (VBNC) Escherichia coli

    SciTech Connect (OSTI)

    Packard, M M; Shusteff, M; Alocilja, E C

    2012-04-12

    Although bacterial culture remains the gold standard for detection of viable bacteria in environmental specimens, the typical time requirement of twenty-four hours can delay and even jeopardize appropriate public health intervention. In addition, culture is incapable of detecting viable but not culturable (VBNC) species. Conversely, nucleic acid and antibody-based methods greatly decrease time to detection but rarely characterize viability of the bacteria detected. Through selection by membrane permeability, the method described in this work employs positive dielectrophoresis (pDEP) for separation and purification of viable and VBNC species from water and allows concentration of bacteria for downstream applications.

  1. Nearly Finished Genomes Produced Using Gel Microdroplet Culturing (Seventh Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting 2012)

    ScienceCinema (OSTI)

    Fitzsimmons, Michael [LANL

    2013-01-25

    Michael Fitzsimmons from Los Alamos National Laboratory gives a talk titled "Nearly Finished Genomes Produced Using Gel Microdroplet Culturing" at the 7th Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting held in June, 2012 in Santa Fe, NM.

  2. Ecological and Cultural Importance of a Species at Risk of Extinction, Pacific Lamprey, 1964-2002 Technical Report.

    SciTech Connect (OSTI)

    Close, David A.

    2002-07-01

    The cultural and ecological values of Pacific lamprey (Lampetra tridentata) have not been understood by Euro-Americans and thus their great decline has almost gone unnoticed except by Native Americans, who elevated the issue and initiated research to restore its populations, at least in the Columbia Basin. They regard Pacific lamprey as a highly valued resource and as a result ksuyas (lamprey) has become one of their cultural icons. Ksuyas are harvested to this day as a subsistence food by various tribes along the Pacific coast and are highly regarded for their cultural value. Interestingly, our review suggests that the Pacific lamprey plays an important role in the food web, may have acted as a buffer for salmon from predators, and may have been an important source of marine nutrients to oligotrophic watersheds. This is very different from the Euro-American perception that lampreys are pests. We suggest that cultural biases affected management policies.

  3. Presentation, Safety from the Operator's Perspective: We are All in This Together, Safety Culture in All We Do

    Broader source: Energy.gov [DOE]

    A presentation by Jim Ellis, President and CEO, Institute of Nuclear Power Operators (INPO), Safety from the Operator’s Perspective: We Are All in this Together, Safety Culture in All We Do.

  4. Design, Fabrication, and Operation of Innovative Microalgae Culture Experiments for the Purpose of Producing Fuels: Final Report, Phase I

    SciTech Connect (OSTI)

    Not Available

    1985-01-01

    A conceptual design was developed for a 1000-acre (water surface) algae culture facility for the production of fuels. The system is modeled after the shallow raceway system with mixing foils that is now being operated at the University of Hawaii. A computer economic model was created to calculate the discounted breakeven price of algae or fuels produced by the culture facility. A sensitivity analysis was done to estimate the impact of changes in important biological, engineering, and financial parameters on product price.

  5. ADAPTIVE MANAGEMENT AND PLANNING MODELS FOR CULTURAL RESOURCES IN OIL & GAS FIELDS IN NEW MEXICO AND WYOMING

    SciTech Connect (OSTI)

    Peggy Robinson

    2005-07-01

    This report summarizes activities that have taken place in the last six (6) months (January 2005-June 2005) under the DOE-NETL cooperative agreement ''Adaptive Management and Planning Models for Cultural Resources in Oil and Gas Fields, New Mexico and Wyoming'' DE-FC26-02NT15445. This project examines the practices and results of cultural resource investigation and management in two different oil and gas producing areas of the United States: southeastern New Mexico and the Powder River Basin of Wyoming. The project evaluates how cultural resource investigations have been conducted in the past and considers how investigation and management could be pursued differently in the future. The study relies upon full database population for cultural resource inventories and resources and geomorphological studies. These are the basis for analysis of cultural resource occurrence, strategies for finding and evaluating cultural resources, and recommendations for future management practices. Activities can be summarized as occurring in either Wyoming or New Mexico. Gnomon as project lead, worked in both areas.

  6. Endangered species and cultural resources program, Naval Petroleum Reserves in California: Annual report FY95

    SciTech Connect (OSTI)

    1996-04-01

    In FY95, EG and G Energy Measurements, Inc. (EG and G/EM) continued to support efforts to protect endangered species and cultural resources at the Naval Petroleum Reserves in California (NPRC). These efforts are conducted to ensure NPRC compliance with regulations regarding the protection of listed species and cultural resources on Federal properties. Population monitoring activities are conducted annually for San Joaquin kit foxes, giant kangaroo rats, blunt-nosed leopard lizards, and Hoover`s wooly-star. To mitigate impacts of oil field activities on listed species, 674 preactivity surveys covering approximately 211 hectares (521 acres) were conducted in FY95. EG and G/EM also assisted with mitigating effects from third-party projects, primarily by conducting biological and cultural resource consultations with regulatory agencies. EG and G/EM has conducted an applied habitat reclamation program at NPRC since 1985. In FY95, an evaluation of revegetation rates on reclaimed and non-reclaimed disturbed lands was completed, and the results will be used to direct future habitat reclamation efforts at NPRC. In FY95, reclamation success was monitored on 50 sites reclaimed in 1985. An investigation of factors influencing the distribution and abundance of kit foxes at NPRC was initiated in FY94. Factors being examined include habitat disturbance, topography, grazing, coyote abundance, lagomorph abundance, and shrub density. This investigation continued in FY95 and a manuscript on this topic will be completed in FY96. Also, Eg and G/EM completed collection of field data to evaluate the effects of a well blow-out on plant and animal populations. A final report will be prepared in FY96. Finally, EG and G/EM completed a life table analysis on San Joaquin kit foxes at NPRC.

  7. The Biofuels Revolution: Understanding the Social, Cultural and Economic Impacts of Biofuels Development on Rural Communities

    SciTech Connect (OSTI)

    Selfa, Theresa L; Goe, Richard; Kulcsar, Laszlo; Middendorf, Gerad; Bain, Carmen

    2013-02-11

    The aim of this research was an in-depth analysis of the impacts of biofuels industry and ethanol plants on six rural communities in the Midwestern states of Kansas and Iowa. The goal was to provide a better understanding of the social, cultural, and economic implications of biofuels development, and to contribute to more informed policy development regarding bioenergy.Specific project objectives were: 1. To understand how the growth of biofuel production has affected and will affect Midwestern farmers and rural communities in terms of economic, demographic, and socio-cultural impacts; 2. To determine how state agencies, groundwater management districts, local governments and policy makers evaluate or manage bioenergy development in relation to competing demands for economic growth, diminishing water resources, and social considerations; 3. To determine the factors that influence the water management practices of agricultural producers in Kansas and Iowa (e.g. geographic setting, water management institutions, competing water-use demands as well as producers attitudes, beliefs, and values) and how these influences relate to bioenergy feedstock production and biofuel processing; 4. To determine the relative importance of social-cultural, environmental and/or economic factors in the promotion of biofuels development and expansion in rural communities; The research objectives were met through the completion of six detailed case studies of rural communities that are current or planned locations for ethanol biorefineries. Of the six case studies, two will be conducted on rural communities in Iowa and four will be conducted on rural communities in Kansas. A multi-method or mixed method research methodology was employed for each case study.

  8. Culturally relevant science: An approach to math science education for Hispanics. Final technical report

    SciTech Connect (OSTI)

    Ortiz de Montellano, B.

    1996-11-14

    As planned a letter was sent out to 17 teachers who had participated in a Summer 1994 workshop on ``Culturally Relevant Science for Hispanics`` at Michigan State. These teachers were supposed to have spent the intervening time developing lesson plans and curricula. The letter requested a report of any activities undertaken and copies of lesson plans and materials developed by February 1996 with a stipend of $400 for satisfactory reports. It was a disappointment to only get 9 responses and not all of them demonstrating a satisfactory level of activity. Diana Marinez, Dean of Science at Texas A and M University, Corpus Christi, who is the other developer of this curriculum and the author reviewed the submitted materials and chose those showing the most promise to be invited to participate in the Summer Writing Workshop. Spring of 1996 and particularly in May--June, the author wrote a partial first draft of a companion volume for the teacher`s manual which would provide a rationale for doing culturally relevant science, present the cultural and the scientific background that teachers would need in order to be able to teach. One of the goals of this curriculum is that it should be off-the-shelf ready to teach and that teachers would not have to do extra research to encourage its adoption. The outline of the book is appendix 1. The Writing Workshop was held at Texas A and M University, Corpus Christi from July 14 to July 27, 1996. Participating teachers chose topics that they were interested in developing and wrote first drafts. These were distributed to all participants and critiqued by the workshop directors before being rewritten. Some teachers were more productive than others depending on their science background. In total an impressive number of lesson plans were written. These lesson plans are listed in Appendix 3. Appendix 4 is a sample lesson. Work still needs to be done on both the source book and the teachers` manual.

  9. Psychosocial and Cultural Modeling in Human Computation Systems: A Gamification Approach

    SciTech Connect (OSTI)

    Sanfilippo, Antonio P.; Riensche, Roderick M.; Haack, Jereme N.; Butner, R. Scott

    2013-11-20

    “Gamification”, the application of gameplay to real-world problems, enables the development of human computation systems that support decision-making through the integration of social and machine intelligence. One of gamification’s major benefits includes the creation of a problem solving environment where the influence of cognitive and cultural biases on human judgment can be curtailed through collaborative and competitive reasoning. By reducing biases on human judgment, gamification allows human computation systems to exploit human creativity relatively unhindered by human error. Operationally, gamification uses simulation to harvest human behavioral data that provide valuable insights for the solution of real-world problems.

  10. Influence of ethanol on fatty acid composition of phospholipids in cultured neurons

    SciTech Connect (OSTI)

    Morrisson, M.; Wilce, P.A.; Shanley, B.C.

    1984-07-31

    Animals chronically exposed to ethanol show changes in neural membrane lipids which may underlie the development of tolerance and physical dependence. The object of this study was to investigate changes in the fatty acid composition of neuronal phospholipids cultured in the presence of ethanol (55 or 110 mM) for periods up to 7 days. Decreases were observed in the percentage of individual and total saturated fatty acids, while the double bond index: total saturated fatty acid ratio, increased. These changes do not support the hypothesis that neural membrane lipid composition changes to counteract the fluidizing action of ethanol. 13 references, 2 tables.

  11. Culturally relevant science: An approach to math science education for Hispanics

    SciTech Connect (OSTI)

    Montellano, B.O. de

    1996-11-14

    This report describes later stages of a program to develop culturally relevant science and math programs for Hispanic students. Part of this effort was follow-up with 17 teachers who participated in early stages of the program. Response was not very good. Included with the report is a first draft effort for curriculum materials which could be used as is in such a teaching effort. Several of the participating teachers were invited to a writing workshop, where lesson plans were drafted, and critiqued and following rework are listed in this publication. Further work needs to be completed and is ongoing.

  12. Endangered species and cultural resources program Naval petroleum Reserves in California. Annual report FY96

    SciTech Connect (OSTI)

    1997-07-01

    In FY96, Enterprise Advisory Services, Inc. (EASI) continued to support efforts to protect endangered species and cultural resources at the Naval Petroleum Reserves in California (NPRC). These efforts are conducted to ensure NPRC compliance with regulations regarding the protection of listed species and cultural resources on federal properties. Population monitoring activities were conducted for San Joaquin kit foxes, giant kangaroo rats, blunt-nosed leopard lizards, and Hoover`s wooly-star. Kit fox abundance and distribution was assessed by live-trapping over a 329-km{sup 2} area. Kit fox reproduction and mortality were assessed by radiocollaring and monitoring 22 adults and two pups. Reproductive success and litter size were determined through live-trapping and den observations. Rates and sources of kit fox mortality were assessed by recovering dead radiocollared kit foxes and conducting necropsies to determine cause of death. Abundance of coyotes and bobcats, which compete with kit foxes, was determined by conducting scent station surveys. Kit fox diet was assessed through analysis of fecal samples collected from live-trapped foxes. Abundance of potential prey for kit foxes was determined by conducting transect surveys for lagornorphs and live-trapping small mammals.

  13. Historic, enthnohistoric and prehistoric cultural resource inventory. Final technical report, November 1980-May 1982

    SciTech Connect (OSTI)

    Not Available

    1982-01-01

    The goal of this study is to provide a literature search and write a historical narrative of the cultural significance of the study area for the proposed WyCoalGas Inc., pipeline, railroad, well fields, and coal gasification plant. The request for a cultural resource investigation states at a minimum the study shall be a literature search on the narrow one mile corridor along the proposed pipelines, areas included within the various facilities plus a one mile buffer surrounding these facilities. In addition, the study must be tied into appropriate local, state, and national history. The writer of this history has felt a responsibility for providing a realistic assessment of the themes of the study area's historical development. Several ideas have been concentrated upon: its American Indian heritage; the Euro-American's exploitive relationship with the region; and the overriding fragile, arid nature of its land. It is hoped that the government agencies and ultimately the energy company will feel a similiar responsibility toward the study area's historical integrity.

  14. Annual review of cultural resource investigations by the Savannah River Archaeological Research Program. Fiscal year 1995

    SciTech Connect (OSTI)

    Brooks, M.J.; Brooks, R.D.; Sassaman, K.E.; Crass, D.C.

    1995-10-01

    The Savannah River Archaeological Research Program (SRARP) continued through FY95 with the United States Department of Energy to fulfill a threefold mission of cultural resource management, research, and public education at the Savannah River Site. Over 2,300 acres of land on the SRS came under cultural resources review in FY95. This activity entailed 30 field surveys, resulting in the recording of 86 new sites. Twenty-two existing sites within survey tract boundaries were revisited to update site file records. Research conducted by SRARP was reported in 11 papers and monographs published during FY95. SRARP staff also presented research results in 18 papers at professional meetings. Field research included several testing programs, excavations, and remote sensing at area sites, as well as data collection abroad. Seven grants were acquired by SRARP staff to support off-site research. In the area of heritage education, the SRARP expanded its activities in FY95 with a full schedule of classroom education, public outreach, and on-site tours. Volunteer excavations at the Tinker Creek site were continued with the Augusta Archaeological Society and other avocational groups, and other off-site excavations provided a variety of opportunities for field experience. Some 80 presentations, displays and tours were provided for schools, historical societies, civic groups, and environmental and historical awareness day celebrations. Additionally, SRARP staff taught four anthropology courses at area colleges.

  15. Functional dissection of the Hox protein Abdominal-B in Drosophila cell culture

    SciTech Connect (OSTI)

    Zhai, Zongzhao [Key Laboratory of the Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beichen West Road, Chaoyang, Beijing 100101 (China) [Key Laboratory of the Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beichen West Road, Chaoyang, Beijing 100101 (China); CellNetworks - Cluster of Excellence, Centre for Organismal Studies (COS) Heidelberg, University of Heidelberg, D-69120 Heidelberg (Germany); Graduate School of Chinese Academy of Sciences, Beijing 100039 (China); Yang, Xingke, E-mail: yangxk@ioz.ac.cn [Key Laboratory of the Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beichen West Road, Chaoyang, Beijing 100101 (China)] [Key Laboratory of the Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beichen West Road, Chaoyang, Beijing 100101 (China); Lohmann, Ingrid, E-mail: ilohmann@flydev.org [CellNetworks - Cluster of Excellence, Centre for Organismal Studies (COS) Heidelberg, University of Heidelberg, D-69120 Heidelberg (Germany)] [CellNetworks - Cluster of Excellence, Centre for Organismal Studies (COS) Heidelberg, University of Heidelberg, D-69120 Heidelberg (Germany)

    2011-11-04

    Highlights: Black-Right-Pointing-Pointer ct340 CRM was identified to be the posterior spiracle enhancer of gene cut. Black-Right-Pointing-Pointer ct340 is under the direct transcriptional control of Hox protein Abd-B. Black-Right-Pointing-Pointer An efficient cloning system was developed to assay protein-DNA interaction. Black-Right-Pointing-Pointer New features of Abd-B dependent target gene regulation were detected. -- Abstract: Hox transcription factors regulate the morphogenesis along the anterior-posterior (A/P) body axis through the interaction with small cis-regulatory modules (CRMs) of their target gene, however so far very few Hox CRMs are known and have been analyzed in detail. In this study we have identified a new Hox CRM, ct340, which guides the expression of the cell type specification gene cut (ct) in the posterior spiracle under the direct control of the Hox protein Abdominal-B (Abd-B). Using the ct340 enhancer activity as readout, an efficient cloning system to generate VP16 activation domain fusion protein was developed to unambiguously test protein-DNA interaction in Drosophila cell culture. By functionally dissecting the Abd-B protein, new features of Abd-B dependent target gene regulation were detected. Due to its easy adaptability, this system can be generally used to map functional domains within sequence-specific transcriptional factors in Drosophila cell culture, and thus provide preliminary knowledge of the protein functional domain structure for further in vivo analysis.

  16. Biohydrogen production from rotten orange with immobilized mixed culture: Effect of immobilization media for various composition of substrates

    SciTech Connect (OSTI)

    Damayanti, Astrilia; Sarto,; Syamsiah, Siti; Sediawan, Wahyudi B.

    2015-12-29

    Enriched–immobilized mixed culture was utilized to produce biohydrogen in mesophilic condition under anaerobic condition using rotten orange as substrate. The process was conducted in batch reactors for 100 hours. Microbial cultures from three different sources were subject to a series of enrichment and immobilized in two different types of media, i.e. calcium alginate (CA, 2%) and mixture of alginate and activated carbon (CAC, 1:1). The performance of immobilized culture in each media was tested for biohydrogen production using four different substrate compositions, namely orange meat (OM), orange meat added with peel (OMP), orange meat added with limonene (OML), and mixture of orange meat and peel added with limonene (OMPL). The results show that, with immobilized culture in CA, the variation of substrate composition gave significant effect on the production of biohydrogen. The highest production of biohydrogen was detected for substrate containing only orange meet, i.e. 2.5%, which was about 3-5 times higher than biohydrogen production from other compositions of substrate. The use of immobilized culture in CAC in general has increased the hydrogen production by 2-7 times depending on the composition of substrate, i.e. 5.4%, 4.8%, 5.1%, and 4.4% for OM, OMP, OML, and OMPL, respectively. The addition of activated carbon has eliminated the effect of inhibitory compounds in the substrate. The major soluble metabolites were acetic acid, propionic acid, and butyric acid.

  17. An Approach for Assessing the Signature Quality of Various Chemical Assays when Predicting the Culture Media Used to Grow Microorganisms

    SciTech Connect (OSTI)

    Holmes, Aimee E.; Sego, Landon H.; Webb-Robertson, Bobbie-Jo M.; Kreuzer, Helen W.; Anderson, Richard M.; Unwin, Stephen D.; Weimar, Mark R.; Tardiff, Mark F.; Corley, Courtney D.

    2013-02-01

    We demonstrate an approach for assessing the quality of a signature system designed to predict the culture medium used to grow a microorganism. The system was comprised of four chemical assays designed to identify various ingredients that could be used to produce the culture medium. The analytical measurements resulting from any combination of these four assays can be used in a Bayesian network to predict the probabilities that the microorganism was grown using one of eleven culture media. We evaluated combinations of the signature system by removing one or more of the assays from the Bayes network. We measured and compared the quality of the various Bayes nets in terms of fidelity, cost, risk, and utility, a method we refer to as Signature Quality Metrics

  18. Cultural Resource Assessment of the Test Area North Demolition Landfill at the Idaho National Engineering and Environmental Laboratory

    SciTech Connect (OSTI)

    Brenda R. Pace

    2003-07-01

    The proposed new demolition landfill at Test Area North on the Idaho National Engineering and Environmental Laboratory (INEEL) will support ongoing demolition and decontamination within the facilities on the north end of the INEEL. In June of 2003, the INEEL Cultural Resource Management Office conducted archival searches, field surveys, and coordination with the Shoshone-Bannock Tribes to identify all cultural resources that might be adversely affected by the project and to provide recommendations to protect those listed or eligible for listing on the National Register of Historic Places. These investigations showed that landfill construction and operation would affect two significant cultural resources. This report outlines protective measures to ensure that these effects are not adverse.

  19. Algal Testbed Public Private Partnerships Workshop on Principles and Processes: Algae Culture Management, Production and Downstream Harvesting

    Broader source: Energy.gov [DOE]

    The Spring 2016 ATP3 workshop will occur May 16th-20th at Santa Fe Community College (SFCC) and the Los Alamos National Lab's New Mexico Consortium (LANL NMC). These unique facilities will give participants incredible insights into aspects across the algae value chain and the food, energy and water nexus. Lectures will cover the fundamentals of managing microalgal cultures, culturing techniques, measuring and analyzing biomass, harvesting and processing technologies, as well as life cycle analysis and operations at the production scale. Participants will have opportunities to work in the laboratory and learn how to measure culture density (cell counting and optical density), use a light and fluorescence microscope, use flow cytometry, and perform gravimetric analyses (dry weight and ash-free dry weight), and techniques necessary to analyze biomass compounds.

  20. Algae Testbed Public-Private Partnership Workshop on Principles and Processes: Algae Culture Management, Production and Downstream Harvesting

    Office of Energy Efficiency and Renewable Energy (EERE)

    The spring 2016 Algae Testbed Public-Private Partnership (ATP3) workshop will occur May 16–20, 2016, at Santa Fe Community College and Los Alamos National Laboratory's New Mexico Consortium Biological Laboratory. These unique facilities will give participants incredible insights into aspects across the algae value chain and the food, energy, and water nexus. Lectures will cover the fundamentals of managing microalgal cultures, culturing techniques, measuring and analyzing biomass, harvesting and processing technologies, and life-cycle analysis and operations at the production scale. Participants will have opportunities to work in the laboratory and learn how to measure culture density (cell counting and optical density), use a light and fluorescence microscope, use flow cytometry, and perform gravimetric analyses (dry weight and ash-free dry weight) and techniques necessary to analyze biomass compounds.

  1. ADAPTIVE MANAGEMENT AND PLANNING MODELS FOR CULTURAL RESOURCES IN OIL & GAS FIELDS IN NEW MEXICO AND WYOMING

    SciTech Connect (OSTI)

    Peggy Robinson

    2004-07-01

    This report contains a summary of activities of Gnomon, Inc. and five subcontractors that have taken place during the first six months of 2004 (January 1, 2004-June 30, 2004) under the DOE-NETL cooperative agreement: ''Adaptive Management and Planning Models for Cultural Resources in Oil & Gas Fields in New Mexico and Wyoming'', DE-FC26-02NT15445. Although Gnomon and all five subcontractors completed tasks during these six months, most of the technical experimental work was conducted by the subcontractor, SRI Foundation (SRIF). SRIF created a sensitivity model for the Azotea Mesa area of southeastern New Mexico that rates areas as having a very good chance, a good chance, or a very poor chance of containing cultural resource sites. SRIF suggested that the results of the sensitivity model might influence possible changes in cultural resource management (CRM) practices in the Azote Mesa area of southeastern New Mexico.

  2. Cell culture arrays using micron-sized ferromagnetic ring-shaped thin films

    SciTech Connect (OSTI)

    Huang, Chen-Yu; Wei, Zung-Hang; Lai, Mei-Feng; Ger, Tzong-Rong

    2015-05-07

    Cell patterning has become an important technology for tissue engineering. In this research, domain walls are formed at the two ends of a ferromagnetic ring thin film after applying a strong external magnetic field, which can effectively attract magnetically labeled cells and control the position for biological cell. Magnetophoresis experiment was conducted to quantify the magnetic nanoparticle inside the cells. A ring-shaped magnetic thin films array was fabricated through photolithography. It is observed that magnetically labeled cells can be successfully attracted to the two ends of the ring-shaped magnetic thin film structure and more cells were attracted and further attached to the structures. The cells are co-cultured with the structure and kept proliferating; therefore, such ring thin film can be an important candidate for in-vitro biomedical chips or tissue engineering.

  3. Production of bio-based materials using photobioreactors with binary cultures

    DOE Patents [OSTI]

    Beliaev, Alex S; Pinchuk, Grigoriy E; Hill, Eric A; Fredrickson, Jim K

    2013-08-27

    A method, device and system for producing preselected products, (either finished products or preselected intermediary products) from biobased precursors or CO.sub.2 and/or bicarbonate. The principal features of the present invention include a method wherein a binary culture is incubated with a biobased precursor in a closed system to transform at least a portion of the biobased precursor to a preselected product. The present invention provides a method of cultivation that does not need sparging of a closed bioreactor to remove or add a gaseous byproduct or nutrient from a liquid medium. This improvement leads to significant savings in energy consumption and allows for the design of photobioreactors of any desired shape. The present invention also allows for the use of a variety of types of waste materials to be used as the organic starting material.

  4. DOE Tribal Intern Focuses on Integrating Energy Policy and Navajo Cultural Values

    Office of Energy Efficiency and Renewable Energy (EERE)

    My interest in energy planning on the Navajo Nation stemmed from the desire to improve energy resource development in my own community. The legacy of environmental and health impacts from uranium and coal mining motivated me to find ways of developing energy resources in a manner more consistent with cultural values and the visions of the Navajo Nation. While I have developed many valuable technical analysis skills and tools in my Ph.D. program, most of the knowledge about Indian energy issues requires significant on-the-ground engagement with communities and their leaders. As a Tribal Energy Program intern, I have developed important connections in the area of tribal energy working with tribal leaders and my fellow interns.

  5. Cultural Resource Investigations for the Resumption of Transient Testing of Nuclear Fuels and Material at the Idaho National Laboratory

    SciTech Connect (OSTI)

    Brenda R. Pace; Julie B. Williams

    2013-11-01

    The U. S. Department of Energy (DOE) has a need to test nuclear fuels under conditions that subject them to short bursts of intense, high-power radiation called ‘transient testing’ in order to gain important information necessary for licensing new nuclear fuels for use in U.S. nuclear power plants, for developing information to help improve current nuclear power plant performance and sustainability, for improving the affordability of new generation reactors, for developing recyclable nuclear fuels, and for developing fuels that inhibit any repurposing into nuclear weapons. To meet this mission need, DOE is considering alternatives for re-use and modification of existing nuclear reactor facilities to support a renewed transient testing program. One alternative under consideration involves restarting the Transient Reactor Test (TREAT) reactor located at the Materials and Fuels Complex (MFC) on the Idaho National Laboratory (INL) site in southeastern Idaho. This report summarizes cultural resource investigations conducted by the INL Cultural Resource Management Office in 2013 to support environmental review of activities associated with restarting the TREAT reactor at the INL. These investigations were completed in order to identify and assess the significance of cultural resources within areas of potential effect associated with the proposed action and determine if the TREAT alternative would affect significant cultural resources or historic properties that are eligible for nomination to the National Register of Historic Places. No archaeological resources were identified in the direct area of potential effects for the project, but four of the buildings proposed for modifications are evaluated as historic properties, potentially eligible for nomination to the National Register of Historic Places. This includes the TREAT reactor (building #), control building (building #), guardhouse (building #), and warehouse (building #). The proposed re-use of these historic

  6. Safety Series No. 75-INSAG-4, Safety Culture: A report by the International Nuclear Safety Advisory Group, International Atomic Energy Agency

    Broader source: Energy.gov [DOE]

    Safety Series No. 75-INSAG-4, Safety Culture: A report by the International Nuclear Safety Advisory Group, International Atomic Energy Agency, IAEA, 1991

  7. A Framework Approach to Evaluate Cross-Cultural Adaptation of Public Engagement Strategies for Radioactive Waste Management - 13430

    SciTech Connect (OSTI)

    Hermann, Laura

    2013-07-01

    The complex interplay of politics, economics and culture undermines attempts to define universal best practices for public engagement in the management of nuclear materials. In the international context, communicators must rely on careful adaptation and creative execution to make standard communication techniques succeed in their local communities. Nuclear professionals need an approach to assess and adapt culturally specific public engagement strategies to meet the demands of their particular political, economic and social structures. Using participant interviews and public sources, the Potomac Communications Group reviewed country-specific examples of nuclear-related communication efforts to provide insight into a proposed approach. The review considered a spectrum of cultural dimensions related to diversity, authority, conformity, proximity and time. Comparisons help to identify cross-cultural influences of various public engagement tactics and to inform a framework for communicators. While not prescriptive in its application, the framework offers a way for communicators to assess the salience of outreach tactics in specific situations. The approach can guide communicators to evaluate and tailor engagement strategies to achieve localized public outreach goals. (authors)

  8. Method for sustaining microorganism culture in syngas fermentation process in decreased concentration or absence of various substrates

    DOE Patents [OSTI]

    Adams, Stephen S.; Scott, Syrona; Ko, Ching-Whan

    2015-05-19

    The present invention relates to methods for sustaining microorganism culture in a syngas fermentation reactor in decreased concentration or absence of various substrates comprising: adding carbon dioxide and optionally alcohol; maintaining free acetic acid concentrations; and performing the above mentioned steps within specified time.

  9. The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Davis, Ryan W.; Carvalho, Benjamin J.; Jones, Howland D. T.; Singh, Seema

    2014-05-13

    Great efforts have been made to elucidate the phenotypic responses of alga to varying levels of nutrients, osmotic environments, and photosynthetically active radiation intensities, though the role of interactions among these variables is largely nebulous. We also describe a general method for establishing and maintaining semi-continuous cultures of the halophilic microalgal production strain, Dunaliella viridis, that is independent of variations in salinity and illumination intensity. Using this method, the cultures were evaluated to elucidate the overlapping roles of photosynthetic and osmotic adaptation on the accumulation and compositional variation of the biomass, photosynthetic productivity, and physiological biomarkers, as well as spectroscopicmore » and morphological details at the single-cell level. Correlation matrices defining the relationships among the observables and based on variation of the illumination intensity and salinity were constructed for predicting bioproduct yields for varying culture conditions. Following maintenance of stable cultures for 6-week intervals, phenotypic responses to photo-osmotic drift were explored using a combination of single-cell hyperspectral fluorescence imaging and flow cytometry. In addition to morphological changes, release of lipid microparticles from the cells that is disproportionate to cell lysis was observed under hypotonic drift, indicating the existence of a reversible membrane permeation mechanism in Dunaliella. Furthermore, this phenomenon introduces the potential for low-cost strategies for recovering lipids and pigments from the microalgae by minimizing the requirement for energy intensive harvesting and dewatering of the biomass. The results should be applicable to outdoor culture, where seasonal changes resulting in variable solar flux and precipitation and evaporation rates are anticipated.« less

  10. The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis

    SciTech Connect (OSTI)

    Davis, Ryan W.; Carvalho, Benjamin J.; Jones, Howland D. T.; Singh, Seema

    2014-05-13

    Great efforts have been made to elucidate the phenotypic responses of alga to varying levels of nutrients, osmotic environments, and photosynthetically active radiation intensities, though the role of interactions among these variables is largely nebulous. We also describe a general method for establishing and maintaining semi-continuous cultures of the halophilic microalgal production strain, Dunaliella viridis, that is independent of variations in salinity and illumination intensity. Using this method, the cultures were evaluated to elucidate the overlapping roles of photosynthetic and osmotic adaptation on the accumulation and compositional variation of the biomass, photosynthetic productivity, and physiological biomarkers, as well as spectroscopic and morphological details at the single-cell level. Correlation matrices defining the relationships among the observables and based on variation of the illumination intensity and salinity were constructed for predicting bioproduct yields for varying culture conditions. Following maintenance of stable cultures for 6-week intervals, phenotypic responses to photo-osmotic drift were explored using a combination of single-cell hyperspectral fluorescence imaging and flow cytometry. In addition to morphological changes, release of lipid microparticles from the cells that is disproportionate to cell lysis was observed under hypotonic drift, indicating the existence of a reversible membrane permeation mechanism in Dunaliella. Furthermore, this phenomenon introduces the potential for low-cost strategies for recovering lipids and pigments from the microalgae by minimizing the requirement for energy intensive harvesting and dewatering of the biomass. The results should be applicable to outdoor culture, where seasonal changes resulting in variable solar flux and precipitation and evaporation rates are anticipated.

  11. National Register of Historic Places multiple property documentation form -- Historic, archaeological, and traditional cultural properties of the Hanford Site, Washington

    SciTech Connect (OSTI)

    Nickens, P.R.

    1997-08-01

    The US Department of Energy`s Hanford Site encompasses an area of 560 square miles on the Columbia River in southeastern Washington. Since 1943, the Hanford Site has existed as a protected area for activities primarily related to the production of radioactive materials for national defense uses. For cultural resources on the Hanford Site, establishment of the nuclear reservation as a high security area, with public access restricted, has resulted in a well-protected status, although no deliberate resource protection measures were in effect to mitigate effects of facilities construction and associated activities. Thus, the Hanford Site contains an extensive record of aboriginal archaeological sites and Native American cultural properties, along with pre-Hanford Euro-American sites (primarily archaeological in nature with the removal of most pre-1943 structures), and a considerable number of Manhattan Project/Cold War era buildings and structures. The recent mission change from production to clean up and disposal of DOE lands created a critical need for development and implementation of new and different cultural resource management strategies. DOE-RL has undertaken a preservation planning effort for the Hanford Site. The intent of this Plan is to enable DOE-RL to organize data and develop goals, objectives, and priorities for the identification, evaluation, registration, protection, preservation, and enhancement of the Site`s historical and cultural properties. Decisions made about the identification, evaluation, registration and treatment of historic properties are most aptly made when relationships between individual properties and other similar properties are considered. The historic context and the multiple property documentation (NTD) process provides DOE-RL the organizational framework for these decisions. Once significant patterns are identified, contexts developed, and expected properties are defined, the NTD process provides the foundation for future

  12. DOE - Office of Legacy Management -- Armour Fertilizer Works...

    Office of Legacy Management (LM)

    Designated Name: Not Designated Alternate Name: U. S. S. Agri-Chemical U.S. Steel ... - Report; Preliminary Survey of U.S. Steel Corporation--Agri-Chemical (Former Armour ...

  13. Endangered species and cultural resources program, Naval Petroleum Reserves in California, annual report FY97

    SciTech Connect (OSTI)

    1998-05-01

    The Naval Petroleum Reserves in California (NPRC) are oil fields administered by the DOE in the southern San Joaquin Valley of California. Four federally endangered animal species and one federally threatened plant species are known to occur on NPRC: San Joaquin kit fox (Vulpes macrotis mutica), blunt-nosed leopard lizard (Gambelia silus), giant kangaroo rat (Dipodomys ingens), Tipton kangaroo rat (Dipodomys nitratoides), and Hoover`s wooly-star (Eriastrum hooveri). All five are protected under the Endangered Species Act (ESA) of 1973. The DOE/NPRC is obliged to determine whether actions taken by their lessees on Naval Petroleum Reserve No. 2 (NPR-2) will have any effects on endangered species or their habitats. The primary objective of the Endangered Species and Cultural Resources Program is to provide NPRC with the scientific expertise necessary for compliance with the ESA, the National Environmental Policy Act (NEPA), and the National Historic Preservation Act (NHPA). The specific objective of this report is to summarize progress, results, and accomplishments of the program during fiscal year 1997 (FY97).

  14. Estrogen inhibits cell cycle progression and retinoblastoma phosphorylation in rhesus ovarian surface epithelial cell culture

    SciTech Connect (OSTI)

    Wright, Jay W.; Stouffer, Richard L.; Rodland, Karin D.

    2003-10-31

    Estrogen promotes the growth of some ovarian cancer cells at nanomolar concentrations, but has been shown to inhibit growth of normal ovarian surface epithelial (OSE) cells at micromolar concentrations (1?g/ml). OSE cells express the estrogen receptor (ER)-?, and are the source of 90% of various cancers. The potential sensitivity of OSE cells to estrogen stresses the importance of understanding the estrogen-dependent mechanisms at play in OSE proliferation and transformation, as well as in anticancer treatment. We investigated the effects of estradiol on cell proliferation in vitro, and demonstrate an intracellular locus of action of estradiol in cultured rhesus ovarian surface epithelial (RhOSE) cells. We show that ovarian and breast cells are growth-inhibited by micromolar concentration of estradiol and that this inhibition correlates with estrogen receptor expression. We further show that normal rhesus OSE cells do not activate ERK or Akt in response to estradiol nor does estradiol block the ability of serum to stimulate ERK or induce cyclin D expression. Contrarily, estradiol inhibits serum-dependent retinoblastoma protein (Rb) phosphorylation and blocks DNA synthesis. This inhibition does not formally arrest cells and is reversible within hours of estrogen withdrawal. Our data are consistent with growth inhibition by activation of Rb and indicate that sensitivity to hormone therapy in anticancer treatment can be modulated by cell cycle regulators downstream of the estrogen receptor.

  15. Molecular Predictors of 3D Morphogenesis by Breast Cancer Cell Lines in 3D Culture

    SciTech Connect (OSTI)

    Han, Ju; Chang, Hang; Giricz, Orsi; Lee, Genee; Baehner, Frederick; Gray, Joe; Bissell, Mina; Kenny, Paraic; Parvin, Bahram

    2010-02-01

    Correlative analysis of molecular markers with phenotypic signatures is the simplest model for hypothesis generation. In this paper, a panel of 24 breast cell lines was grown in 3D culture, their morphology was imaged through phase contrast microscopy, and computational methods were developed to segment and represent each colony at multiple dimensions. Subsequently, subpopulations from these morphological responses were identified through consensus clustering to reveal three clusters of round, grape-like, and stellate phenotypes. In some cases, cell lines with particular pathobiological phenotypes clustered together (e.g., ERBB2 amplified cell lines sharing the same morphometric properties as the grape-like phenotype). Next, associations with molecular features were realized through (i) differential analysis within each morphological cluster, and (ii) regression analysis across the entire panel of cell lines. In both cases, the dominant genes that are predictive of the morphological signatures were identified. Specifically, PPAR? has been associated with the invasive stellate morphological phenotype, which corresponds to triple-negative pathobiology. PPAR? has been validated through two supporting biological assays.

  16. Transcriptional and metabolic flux profiling of triadimefon effects on cultured hepatocytes

    SciTech Connect (OSTI)

    Iyer, Vidya V.; Ovacik, Meric A.; Androulakis, Ioannis P.; Roth, Charles M.; Ierapetritou, Marianthi G.

    2010-11-01

    Conazoles are a class of azole fungicides used to prevent fungal growth in agriculture, for treatment of fungal infections, and are found to be tumorigenic in rats and/or mice. In this study, cultured primary rat hepatocytes were treated to two different concentrations (0.3 and 0.15 mM) of triadimefon, which is a tumorigenic conazole in rat and mouse liver, on a temporal basis with daily media change. Following treatment, cells were harvested for microarray data ranging from 6 to 72 h. Supernatant was collected daily for three days, and the concentrations of various metabolites in the media and supernatant were quantified. Gene expression changes were most significant following exposure to 0.3 mM triadimefon and were characterized mainly by metabolic pathways related to carbohydrate, lipid and amino acid metabolism. Correspondingly, metabolic network flexibility analysis demonstrated a switch from fatty acid synthesis to fatty acid oxidation in cells exposed to triadimefon. It is likely that fatty acid oxidation is active in order to supply energy required for triadimefon detoxification. In 0.15 mM triadimefon treatment, the hepatocytes are able to detoxify the relatively low concentration of triadimefon with less pronounced changes in hepatic metabolism.

  17. Utilisation of single added fatty acids by consortia of digester sludge in batch culture

    SciTech Connect (OSTI)

    Wagner, Andreas Otto; Gstrauntaler, Gudrun; Illmer, Paul

    2010-10-15

    Inocula derived from an anaerobic digester were used to study (i) their potential for methane production and (ii) the utilisation rates of different short chain fatty acids (SCFAs) by the microbial community in defined media with mono-carbon sources (formic-, acetetic-, propionic-, butyric acid) in batch culture. It could be demonstrated that the microbial reactor population could be transferred successfully to the lab, and its ability to build up methane was present even with deteriorating biogas plant performance. Therefore, this reduction in performance of the biogas plant was not due to a decrease in abundance, but due to an inactivity of the microbial community. Generally, the physico-chemical properties of the biogas plant seemed to favour hydrogenotrophic methanogens, as seen by the high metabolisation rates of formate compared with all other carbon sources. In contrast, acetoclastic methanogenesis could be shown to play a minor role in the methane production of the investigated biogas plant, although the origin of up to 66% of methane is generally suggested to be generated through acetoclastic pathway.

  18. Towards breaking the silence between the two cultures: Engineering and the other humanities

    SciTech Connect (OSTI)

    Prausnitz, John M.

    2003-01-01

    Over the years, I have attended numerous meetings like this one at the Center for the Study of Higher Education. I have noticed that most of the attendees, and certainly the speakers, tend to come from the social sciences or humanities. Only rarely do I see anyone here from Berkeley's College of Chemistry or College of Engineering. I come from the College of Chemistry that includes Berkeley's Department of Chemical Engineering. I mention this background to indicate that my remarks here are necessarily less abstract, less theoretical and less philosophical than those of most previous seminar speakers. My remarks are probably somewhat simplistic because, as a result of my engineering background, I tend to focus less on generalities and principles, giving more attention to possible solutions of limited practical problems. About seven weeks ago, I was invited to attend a conference sponsored by the Berlin Academy of Sciences where ''Sciences'' is not confined to natural sciences but includes also humanities and social sciences. The topic of the Conference was ''Sprachlosigkeit'', a German word that roughly translated means inability to speak. The subtitle was ''Silence Between the Disciplines''. The German universities are worried about the increasing gulf between what is often called ''the two cultures''. This gulf is a problem everywhere, including Berkeley, but it is my impression that it is much worse in Europe than in America. The International Conference in Berlin was attended by some big names including the presidents of the Humboldt University in Berlin, the University of Uppsala in Sweden and the Central European University of Budapest, as well as some distinguished academics from a variety of institutions including Harvard and Stanford, and the presidents of three major funding organizations: The Volkswagen Foundation, The German National Science Foundation and the Max Planck Society. The speakers were primarily from the humanities and social sciences but

  19. Recent developments in atomic/nuclear methodologies used for the study of cultural heritage objects

    SciTech Connect (OSTI)

    Appoloni, Carlos Roberto

    2013-05-06

    Archaeometry is an area established in the international community since the 60s, with extensive use of atomic-nuclear methods in the characterization of art, archaeological and cultural heritage objects in general. In Brazil, however, until the early '90s, employing methods of physics, only the area of archaeological dating was implemented. It was only after this period that Brazilian groups became involved in the characterization of archaeological and art objects with these methodologies. The Laboratory of Applied Nuclear Physics, State University of Londrina (LFNA/UEL) introduced, pioneered in 1994, Archaeometry and related issues among its priority lines of research, after a member of LFNA has been involved in 1992 with the possibilities of tomography in archaeometry, as well as the analysis of ancient bronzes by EDXRF. Since then, LFNA has been working with PXRF and Portable Raman in several museums in Brazil, in field studies of cave paintings and in the laboratory with material sent by archaeologists, as well as carrying out collaborative work with new groups that followed in this area. From 2003/2004 LAMFI/DFN/IFUSP and LIN/COPPE/UFRJ began to engage in the area, respectively with methodologies using ion beams and PXRF, then over time incorporating other techniques, followed later by other groups. Due to the growing number of laboratories and institutions/archaeologists/conservators interested in these applications, in may 2012 was created a network of available laboratories, based at http://www.dfn.if.usp.br/lapac. It will be presented a panel of recent developments and applications of these methodologies by national groups, as well as a sampling of what has been done by leading groups abroad.

  20. Highly purified hexachlorobenzene induces cytochrome P4501A in primary cultures of chicken embryo hepatocytes

    SciTech Connect (OSTI)

    Mundy, Lukas J.; Jones, Stephanie P.; Crump, Doug; Herve, Jessica C.; Konstantinov, Alex; Utley, Fiona; Potter, David; Kennedy, Sean W.

    2010-11-01

    Some uncertainty exists regarding the purity of hexachlorobenzene (HCB) used in past toxicity studies. It has been suggested that reported toxic and biochemical effects initially attributed to HCB exposure may have actually been elicited by contamination of HCB by polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs). Herein, primary cultures of chicken embryo hepatocytes (CEH) were used to compare the potencies of two lots of reagent-grade hexachlorobenzene (HCB-old [HCB-O] and HCB-new [HCB-N]), highly purified HCB (HCB-P) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as inducers of ethoxyresorufin O-deethylase (EROD) activity, cytochrome P4501A4 (CYP1A4) messenger ribonucleic acid (mRNA) and CYP1A5 mRNA. The study also compared the EROD- and CYP1A4/5 mRNA-inducing potencies of HCB to the potencies of two mono-ortho substituted polychlorinated biphenyls (PCBs), 2,3,3',4,4'-pentachlorobiphenyl (PCB 105) and 2,3'4,4',5-pentachlorobiphenyl (PCB 118). HCB-O, HCB-N and HCB-P all induced EROD activity and up-regulated CYP1A4 and CYP1A5 mRNAs. Induction was not caused by contamination of HCB with PCDDs or PCDFs. Based upon a comparison of the EC{sub 50} and EC{sub threshold} values for EROD and CYP1A4/5 mRNA concentration-response curves, the potency of HCB relative to the potency of TCDD was 0.0001, and was similar to that of PCB 105 and PCB 118. The maximal EROD activity and CYP1A4/5 mRNA expression differed greatly between HCB and TCDD, and may contribute to an overestimation of the ReP value calculated for highly purified HCB.

  1. Double trisomy mosaic (47,XXX/48,XXX,+13) confirmed by FISH and skin fibroblast culture

    SciTech Connect (OSTI)

    Lieber, E.; Grady, V.; Dosik, H.

    1994-09-01

    A 4 lb 8 oz female was born to a 49-year-old woman (P1200G12) at 40 weeks. The baby had tetralogy of Fallot, polydactyly, microcephaly, low set simple ears, posterior cleft of the soft palate and overlapping flexion deformities of both hands. The eyes were deep set. The clinical impression was trisomy 13. The baby is not doing well and needs a gastrotomy tube for feeding. Sucking is allright but swallowing is impeded. An MRI showed an anomaly of the corpus callosum. The ophthalmological examination showed no abnormalities. A chromosome study on a 2-day peripheral blood sample resulted in poor growth and poor morphology; however, 20 Giemsa-banded cells revealed a 47,XXX karyotype. A second specimen was obtained to search for mosaicism and a blood smear revealed nuclear projections on the neutrophils. FISH analysis using whole chromosome painting probe (Life Technologies) first identified the extra chromosome number 13, the final results showing five of sixty metaphase cells (8.3%) with trisomy 13. Cytogenetic analysis using Giemsa-banding technique revealed four cells in fifty examined (8.0%) with a 48,XXX,+13 karyotype. In order to further evaluate the mosaicism, cytogenetic analysis of a skin fibroblast culture was performed. Twenty one of twenty three cells examined (91.3%) showed the 48,XXX,+13 karyotype. FISH analysis of the skin biopsy revealed eighteen of twenty cells (90.9%) with the trisomy 13. The FISH technique is an important enhancement to routine cytogenetic studies when they do not immediately correlate with clinical impressions.

  2. FROM POLLUTER TO PROTECTOR: THE CHALLENGES OF CHANGING CULTURE, OPERATIONS AND IMAGE

    SciTech Connect (OSTI)

    ZIMMERMAN,E.A.

    2000-07-25

    Brookhaven National Laboratory is a US Department of Energy (DOE) multi-program research facility, located in Suffolk County in Long Island, New York. In 1997, groundwater monitoring revealed significant levels of tritium contamination from a reactor fuel pool. The public reaction was immediate and intense. In an unprecedented move, DOE terminated the contractor and rebid the Laboratory management contract. Brookhaven Science Associates (BSA), a partnership between Battelle and the State University of New York at Stony Brook, won the contract. BSA faced enormous challenges in the environmental area. One was changing the culture and mindset of staff and management with regard to environmental protection. Another was changing operations to fully integrate environmental stewardship into all facets of the Laboratory's missions. And finally, BSA needed to change the Laboratory's public image. This paper describes how BSA faced those challenges. DOE and BSA entered into a voluntary agreement with the US Environmental Protection Agency to conduct an in-depth evaluation of the environmental aspects and impacts of all activities onsite. A project was initiated to explore environmental problems associated with historical activities. BSA also has made significant investments in developing and implementing an Environmental Management System that is consistent with the ISO 14001 standard, with enhancements in the area of compliance assurance. Finally, BSA improved its community involvement program to develop and maintain a positive, proactive and constructive relationship with stakeholders. This paper discusses the approach and results of these efforts. For example, one of major facilities at Brookhaven National Laboratory was recently certified to the ISO 14001 standard, becoming the first Long Island-based organization and the first DOE Office of Science facility to achieve registration.

  3. Effect of acetic acid on lipid accumulation by glucose-fed activated sludge cultures

    SciTech Connect (OSTI)

    Mondala, Andro; Hernandez, Rafael; French, Todd; McFarland, Linda; Sparks, Darrell; Holmes, William; Haque, Monica

    2012-01-01

    The effect of acetic acid, a lignocellulose hydrolysis by-product, on lipid accumulation by activated sludge cultures grown on glucose was investigated. This was done to assess the possible application of lignocellulose as low-cost and renewable fermentation substrates for biofuel feedstock production. Results: Biomass yield was reduced by around 54% at a 2 g L -1 acetic acid dosage but was increased by around 18% at 10 g L -1 acetic acid dosage relative to the control run. The final gravimetric lipid contents at 2 and 10 g L -1 acetic acid levels were 12.5 ± 0.7% and 8.8 ± 3.2% w/w, respectively, which were lower than the control (17.8 ± 2.8% w/w). However, biodiesel yields from activated sludge grown with acetic acid (5.6 ± 0.6% w/w for 2 g L -1 acetic acid and 4.2 ± 3.0% w/w for 10 g L -1 acetic acid) were higher than in raw activated sludge (1-2% w/w). The fatty acid profiles of the accumulated lipids were similar with conventional plant oil biodiesel feedstocks. Conclusions: Acetic acid enhanced biomass production by activated sludge at high levels but reduced lipid production. Further studies are needed to enhance acetic acid utilization by activated sludge microorganisms for lipid biosynthesis.

  4. Breckinridge Project, initial effort. Report VII, Volume III. Cultural resource assessment socioeconomic background data

    SciTech Connect (OSTI)

    Macfarlane, Heather; Janzen, Donald E.

    1980-11-26

    This report has been prepared in conjunction with an environmental baseline study for a commercial coal conversion facility being conducted by Ashland Synthetic Fuels, Inc. (ASFI) and Airco Energy Company (AECO). This report represents a cultural resource assessment for the proposed plant site and two potential solid waste disposal areas. This assessment presents data collected by Dames and Moore during a recent archaeological reconnaissance of the unsurveyed southeastern portion of the proposed plant site and two potential solid waste disposal areas. Also, results of two previous surveys on the northern and southwestern portion of the plant site for American Smelting and Refining Company (ASARCO) and Kentucky Utilities are included. The Dames and Moore survey of the southeastern portion of the plant site identified one archaeological site, three standing structures and one historic cemetery. In addition 47 archaeological sites and six standing structures are known from two previous surveys of the remainder of the plant site (Cowan 1975 and Turnbow et al 1980). Eleven of the previously recorded archaeological sites were recommended for further assessment to evaluate their potential for inclusion within the Holt Bottoms Archaeological District currently listed on the National Register of Historic Places. None of the archaeological sites or standing structures located within the plant site during the Dames and Moore survey were recommended for further assessment. A total of eight archaeological sites were located during the Dames and Moore survey of the two potential solid waste disposal areas. Of this total only two sites were recommended for further assessment. Also, one previously unknown historic cemetry was located in the southernmost potential waste disposal area.

  5. Cultural Resource Investigation for the Materials and Fuels Complex Wastewater System Upgrade at the Idaho National Laboratory

    SciTech Connect (OSTI)

    Brenda R. Pace; Julie B raun Williams; Hollie Gilbert; Dino Lowrey; Julie Brizzee

    2010-05-01

    The Materials and Fuels Complex (MFC) located in Bingham County at the Idaho National Laboratory (INL) in southeastern Idaho is considering several alternatives to upgrade wastewater systems to meet future needs at the facility. In April and May of 2010, the INL Cultural Resource Management Office conducted archival searches, archaeological field surveys, and coordination with the Shoshone-Bannock Tribes to identify cultural resources that may be adversely affected by the proposed construction and to provide recommendations to protect any resources listed or eligible for listing on the National Register of Historic Places. These investigations showed that one National Register-eligible archaeological site is located on the boundary of the area of potential effects for the wastewater upgrade. This report outlines protective measures to help ensure that this resource is not adversely affected by construction.

  6. Construction of an in vitro primary lung co-culture platform derived from New Zealand white rabbits

    SciTech Connect (OSTI)

    Powell, Joshua D.; Hess, Becky M.; Hutchison, Janine R.; Straub, Tim M.

    2015-05-01

    We report the construction of an in vitro three dimensional (3D) co-culture platform consisting of differentiated lung epithelial cells and monocytes from New Zealand white rabbits. Rabbit lung epithelial cells were successfully grown at air-liquid interface, produced mucus, and expressed both sialic acid alpha-2,3 and alpha-2,6. Blood-derived CD14+ monocytes were deposited above the epithelial layer resulting in the differentiation of a subset of monocytes into CD11c+ cells within the co-culture. These proof-of-concept findings provide a convenient means to comparatively study in vitro versus in vivo rabbit lung responses as they relate to inhalation or lung-challenge studies.

  7. Adaptive Management and Planning Models for Cultural Resources in Oil and Gas Fields in New Mexico and Wyoming

    SciTech Connect (OSTI)

    Eckerle, William; Hall, Stephen

    2005-12-30

    In 2002, Gnomon, Inc., entered into a cooperative agreement with the U.S. Department of Energy (DOE) National Energy Technology Laboratory (NETL) for a project entitled, Adaptive Management and Planning Models for Cultural Resources in Oil and Gas Fields in New Mexico and Wyoming (DE-FC26-02NT15445). This project, funded through DOE’s Preferred Upstream Management Practices grant program, examined cultural resource management practices in two major oil- and gas-producing areas, southeastern New Mexico and the Powder River Basin of Wyoming (Figure 1). The purpose of this project was to examine how cultural resources have been investigated and managed and to identify more effective management practices. The project also was designed to build information technology and modeling tools to meet both current and future management needs. The goals of the project were described in the original proposal as follows: Goal 1. Create seamless information systems for the project areas. Goal 2. Examine what we have learned from archaeological work in the southeastern New Mexico oil fields and whether there are better ways to gain additional knowledge more rapidly or at a lower cost. Goal 3. Provide useful sensitivity models for planning, management, and as guidelines for field investigations. Goal 4. Integrate management, investigation, and decision- making in a real-time electronic system. Gnomon, Inc., in partnership with the Wyoming State Historic Preservation Office (WYSHPO) and Western GeoArch Research, carried out the Wyoming portion of the project. SRI Foundation, in partnership with the New Mexico Historic Preservation Division (NMHPD), Statistical Research, Inc., and Red Rock Geological Enterprises, completed the New Mexico component of the project. Both the New Mexico and Wyoming summaries concluded with recommendations how cultural resource management (CRM) processes might be modified based on the findings of this research.

  8. Toxicity of polychlorinated diphenyl ethers in hydra attenuata and in rat whole-embryo culture. Master's thesis

    SciTech Connect (OSTI)

    Becker, M.C.

    1991-05-01

    Polychlorinated diphenyl ethers (PCDEs) are a class of biaryl compounds that have little commercial application, but appear to be widespread in the environment. They have been found in wood preservative waste dumpsites and in fly ash from municipal waste incinerators. They have been detected in bird eggs and tissues, fish, and other edible marine organisms in the United States, Canada, and Europe. There are limited reports in the extant literature on the toxicity of PCDEs. This study was designed to evaluate the toxicity of selected PCDEs in cultures of Hydra attenuata and post-implantation rat whole embryos. The toxicity of several closely related polychlorinated biphenyls (PCBs) was evaluated in both cultures and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was evaluated in whole embryo culture. Embryonic growth and development parameters (yolk sac diameter, crown-rump length, somite count, and DNA and protein content) and gross morphology were determined. Findings indicated that these chemicals were neither embryotoxic nor teratogenic. Thus, the PCDEs, which elicit other diverse toxic and biochemical responses in rodents, are relatively inactive in these bioassays for developmental toxicity.

  9. The usefulness of three-dimensional cell culture in induction of cancer stem cells from esophageal squamous cell carcinoma cell lines

    SciTech Connect (OSTI)

    Fujiwara, Daisuke; Kato, Kazunori; Department of Atopy Research Center, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421 ; Nohara, Shigeo; Iwanuma, Yoshimi; Kajiyama, Yoshiaki

    2013-05-17

    Highlights: Spheroids were created from esophageal carcinoma cells using NanoCulture Plates. The proportion of strongly ALDH-positive cells increased in 3-D culture. Expression of cancer stem cell-related genes was enhanced in 3-D culture. CA-9 expression was enhanced, suggesting hypoxia had been induced in 3-D culture. Drug resistance was increased. 3-D culture is useful for inducing cancer stem cells. -- Abstract: In recent years, research on resistance to chemotherapy and radiotherapy in cancer treatment has come under the spotlight, and researchers have also begun investigating the relationship between resistance and cancer stem cells. Cancer stem cells are assumed to be present in esophageal cancer, but experimental methods for identification and culture of these cells have not yet been established. To solve this problem, we created spheroids using a NanoCulture Plate (NCP) for 3-dimensional (3-D) cell culture, which was designed as a means for experimentally reproducing the 3-D structures found in the body. We investigated the potential for induction of cancer stem cells from esophageal cancer cells. Using flow cytometry we analyzed the expression of surface antigen markers CD44, CD133, CD338 (ABCG2), CD318 (CDCP1), and CD326 (EpCAM), which are known cancer stem cell markers. None of these surface antigen markers showed enhanced expression in 3-D cultured cells. We then analyzed aldehyde dehydrogenase (ALDH) enzymatic activity using the ALDEFLUOR reagent, which can identify immature cells such as stem cells and precursor cells. 3-D-cultured cells were strongly positive for ALDH enzyme activity. We also analyzed the expression of the stem cell-related genes Sox-2, Nanog, Oct3/4, and Lin28 using RT-PCR. Expression of Sox-2, Nanog, and Lin28 was enhanced. Analysis of expression of the hypoxic surface antigen marker carbonic anhydrase-9 (CA-9), which is an indicator of cancer stem cell induction and maintenance, revealed that CA-9 expression was enhanced

  10. Hepatocyte-derived cultured cells with unusual cytoplasmic keratin-rich spheroid bodies

    SciTech Connect (OSTI)

    Delavalle, Pierre-Yves; Alsaleh, Khaled; Pillez, Andre; Cocquerel, Laurence; Universite Lille Nord de France, F-59000 Lille; Institut Pasteur de Lille, F-59019 Lille ; Allet, Cecile; Dumont, Patrick; Institut Pasteur de Lille, F-59019 Lille; CNRS UMR 8161, F-59021 Lille ; Loyens, Anne; Leteurtre, Emmanuelle; Omary, M. Bishr; Dubuisson, Jean; Rouille, Yves; Universite Lille Nord de France, F-59000 Lille; Institut Pasteur de Lille, F-59019 Lille ; Wychowski, Czeslaw; Universite Lille Nord de France, F-59000 Lille; Institut Pasteur de Lille, F-59019 Lille

    2011-11-01

    Cytoplasmic inclusions are found in a variety of diseases that are characteristic morphological features of several hepatic, muscular and neurodegenerative disorders. They display a predominantly filamentous ultrastructure that is also observed in malignant rhabdoid tumor (MRT). A cellular clone containing an intracytoplasmic body was isolated from hepatocyte cell culture, and in the present study we examined whether this body might be related or not to Mallory-Denk body (MDB), a well characterized intracytoplasmic inclusion, or whether this cellular clone was constituted by malignant rhabdoid tumor cells. The intracytoplasmic body was observed in electron microscopy (EM), confocal immunofluorescence microscopy and several proteins involved in the formation of its structure were identified. Using light microscopy, a spheroid body (SB) described as a single regular-shaped cytoplasmic body was observed in cells. During cytokinesis, the SB was disassembled and reassembled in a way to reconstitute a unique SB in each progeny cell. EM examination revealed that the SB was not surrounded by a limiting membrane. However, cytoplasmic filaments were concentrated in a whorled array. These proteins were identified as keratins 8 and 18 (K8/K18), which formed the central core of the SB surrounded by a vimentin cage-like structure. This structure was not related to Mallory-Denk body or aggresome since no aggregated proteins were located in SB. Moreover, the structure of SB was not due to mutations in the primary sequence of K8/K18 and vimentin since no difference was observed in the mRNA sequence of their genes, isolated from Huh-7 and Huh-7w7.3 cells. These data suggested that cellular factor(s) could be responsible for the SB formation process. Aggregates of K18 were relocated in the SB when a mutant of K18 inducing disruption of K8/K18 IF network was expressed in the cellular clone. Furthermore, the INI1 protein, a remodeling-chromatin factor deficient in rhabdoid cells, which

  11. Pond Crash Forensics: Presumptive identification of pond crash agents by next generation sequencing in replicate raceway mass cultures of Nannochloropsis salina

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Carney, Laura T.; Wilkenfeld, Joshua S.; Lane, Pam D.; Solberg, Owen D.; Fuqua, Zachary B.; Cornelius, Nina G.; Gillespie, Shaunette; Williams, Kelly P.; Samocha, Tzachi M.; Lane, Todd W.

    2016-06-02

    Productivity of algal mass culture can be severely reduced by contaminating organisms. It is, therefore, important to identify contaminants, determine their effect on productivity and, ultimately, develop countermeasures against such contamination. In this paper, we utilized microbiome analysis by second-generation sequencing of small subunit rRNA genes to characterize the predator and pathogen burden of open raceway cultures of Nannochloropsis salina. Samples were analyzed from replicate raceways before and after crashes. In one culture cycle, we identified two algivorous species, the rotifer Brachionus and gastrotrich Chaetonotus, the presence of which may have contributed to the loss of algal biomass. In themore » second culture cycle, the raceways were treated with hypochlorite in an unsuccessful attempt to interdict the crash. Finally, our analyses were shown to be an effective strategy for the identification of the biological contaminants and the characterization of intervention strategies.« less

  12. Supplemnental Volume - Independent Oversight Assessment of the Nuclear Safety Culture and Management of Nuclear Safety Concerns at the Hanford Site Waste Treatment and Immobilization Plant, January 2012

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Supplemental Volume Independent Oversight Assessment of Nuclear Safety Culture and Management of Nuclear Safety Concerns at the Hanford Site Waste Treatment and Immobilization Plant January 2012 Office of Enforcement and Oversight Office of Health, Safety and Security U.S. Department of Energy Office of Health, Safety and Security HSS i Independent Oversight Assessment of Safety Culture and Management of Nuclear Safety Concerns at the Hanford Site Waste Treatment and Immobilization Plant

  13. Class I cultural resource overview for oil shale and tar sands areas in Colorado, Utah and Wyoming.

    SciTech Connect (OSTI)

    O'Rourke, D.; Kullen, D.; Gierek, L.; Wescott, K.; Greby, M.; Anast, G.; Nesta, M.; Walston, L.; Tate, R.; Azzarello, A.; Vinikour, B.; Van Lonkhuyzen, B.; Quinn, J.; Yuen, R.; Environmental Science Division

    2007-11-01

    In August 2005, the U.S. Congress enacted the Energy Policy Act of 2005, Public Law 109-58. In Section 369 of this Act, also known as the 'Oil Shale, Tar Sands, and Other Strategic Unconventional Fuels Act of 2005', Congress declared that oil shale and tar sands (and other unconventional fuels) are strategically important domestic energy resources that should be developed to reduce the nation's growing dependence on oil from politically and economically unstable foreign sources. The Bureau of Land Management (BLM) is developing a Programmatic Environmental Impact Statement (PEIS) to evaluate alternatives for establishing commercial oil shale and tar sands leasing programs in Colorado, Wyoming, and Utah. This PEIS evaluates the potential impacts of alternatives identifying BLM-administered lands as available for application for commercial leasing of oil shale resources within the three states and of tar sands resources within Utah. The scope of the analysis of the PEIS also includes an assessment of the potential effects of future commercial leasing. This Class I cultural resources study is in support of the Draft Oil Shale and Tar Sands Resource Management Plan Amendments to Address Land Use Allocations in Colorado, Utah, and Wyoming and Programmatic Environmental Impact Statement and is an attempt to synthesize archaeological data covering the most geologically prospective lands for oil shale and tar sands in Colorado, Utah, and Wyoming. This report is based solely on geographic information system (GIS) data held by the Colorado, Utah, and Wyoming State Historic Preservation Offices (SHPOs). The GIS data include the information that the BLM has provided to the SHPOs. The primary purpose of the Class I cultural resources overview is to provide information on the affected environment for the PEIS. Furthermore, this report provides recommendations to support planning decisions and the management of cultural resources that could be impacted by future oil shale and tar

  14. Effects of FGF-2 on human adipose tissue derived adult stem cells morphology and chondrogenesis enhancement in Transwell culture

    SciTech Connect (OSTI)

    Kabiri, Azadeh; Esfandiari, Ebrahim; Hashemibeni, Batool; Kazemi, Mohammad; Mardani, Mohammad; Esmaeili, Abolghasem

    2012-07-27

    Highlights: Black-Right-Pointing-Pointer We investigated effects of FGF-2 on hADSCs. Black-Right-Pointing-Pointer We examine changes in the level of gene expressions of SOX-9, aggrecan and collagen type II and type X. Black-Right-Pointing-Pointer FGF-2 induces chondrogenesis in hADSCs, which Bullet Increasing information will decrease quality if hospital costs are very different. Black-Right-Pointing-Pointer The result of this study may be beneficial in cartilage tissue engineering. -- Abstract: Injured cartilage is difficult to repair due to its poor vascularisation. Cell based therapies may serve as tools to more effectively regenerate defective cartilage. Both adult mesenchymal stem cells (MSCs) and human adipose derived stem cells (hADSCs) are regarded as potential stem cell sources able to generate functional cartilage for cell transplantation. Growth factors, in particular the TGF-b superfamily, influence many processes during cartilage formation, including cell proliferation, extracellular matrix synthesis, maintenance of the differentiated phenotype, and induction of MSCs towards chondrogenesis. In the current study, we investigated the effects of FGF-2 on hADSC morphology and chondrogenesis in Transwell culture. hADSCs were obtained from patients undergoing elective surgery, and then cultured in expansion medium alone or in the presence of FGF-2 (10 ng/ml). mRNA expression levels of SOX-9, aggrecan and collagen type II and type X were quantified by real-time polymerase chain reaction. The morphology, doubling time, trypsinization time and chondrogenesis of hADSCs were also studied. Expression levels of SOX-9, collagen type II, and aggrecan were all significantly increased in hADSCs expanded in presence of FGF-2. Furthermore FGF-2 induced a slender morphology, whereas doubling time and trypsinization time decreased. Our results suggest that FGF-2 induces hADSCs chondrogenesis in Transwell culture, which may be beneficial in cartilage tissue engineering.

  15. Changing Safety Culture, One Step at a Time: The Value of the DOE-VPP Program at PNNL

    SciTech Connect (OSTI)

    Wright, Patrick A.; Isern, Nancy G.

    2005-02-01

    The primary value of the Pacific Northwest National Laboratory (PNNL) Voluntary Protection Program (VPP) is the ongoing partnership between management and staff committed to change Laboratory safety culture one step at a time. VPP enables PNNL's safety and health program to transcend a top-down, by-the-book approach to safety, and it also raises grassroots safety consciousness by promoting a commitment to safety and health 24 hours a day, 7 days a week. PNNL VPP is a dynamic, evolving program that fosters innovative approaches to continuous improvement in safety and health performance at the Laboratory.

  16. International School of Innovative Technology for Cleaning the Environment, Ettore Majorana Centre for Scientific Culture: Erice, Sicily, Italy

    SciTech Connect (OSTI)

    Ragaini, R.C.

    1994-06-01

    The International School of Innovative Technology for Cleaning the Environment was founded at the Ettore Majorana Centre for Scientific Culture (EMCSC), the seat of the World Laboratory Mediterranean Branch, in 1989. The School primarily organizes and hosts training courses and advanced study courses addressing state-of-the-art technologies to clean the environment, minimize waste generation, prevent pollution, and identify strategies to choose environmentally resilient sites and processes for new industrial installations. The School also participates in facilitating multi-national research projects for developing countries under the auspices of the World Laboratory and other sponsoring agencies.

  17. Alginate based 3D hydrogels as an in vitro co-culture model platform for the toxicity screening of new chemical entities

    SciTech Connect (OSTI)

    Lan, Shih-Feng; Starly, Binil

    2011-10-01

    Prediction of human response to potential therapeutic drugs is through conventional methods of in vitro cell culture assays and expensive in vivo animal testing. Alternatives to animal testing require sophisticated in vitro model systems that must replicate in vivo like function for reliable testing applications. Advancements in biomaterials have enabled the development of three-dimensional (3D) cell encapsulated hydrogels as in vitro drug screening tissue model systems. In this study, we have developed an in vitro platform to enable high density 3D culture of liver cells combined with a monolayer growth of target breast cancer cell line (MCF-7) in a static environment as a representative example of screening drug compounds for hepatotoxicity and drug efficacy. Alginate hydrogels encapsulated with serial cell densities of HepG2 cells (10{sup 5}-10{sup 8} cells/ml) are supported by a porous poly-carbonate disc platform and co-cultured with MCF-7 cells within standard cell culture plates during a 3 day study period. The clearance rates of drug transformation by HepG2 cells are measured using a coumarin based pro-drug. The platform was used to test for HepG2 cytotoxicity 50% (CT{sub 50}) using commercially available drugs which further correlated well with published in vivo LD{sub 50} values. The developed test platform allowed us to evaluate drug dose concentrations to predict hepatotoxicity and its effect on the target cells. The in vitro 3D co-culture platform provides a scalable and flexible approach to test multiple-cell types in a hybrid setting within standard cell culture plates which may open up novel 3D in vitro culture techniques to screen new chemical entity compounds. - Graphical abstract: Display Omitted Highlights: > A porous support disc design to support the culture of desired cells in 3D hydrogels. > Demonstrated the co-culture of two cell types within standard cell-culture plates. > A scalable, low cost approach to toxicity screening involving

  18. Predictivity of dog co-culture model, primary human hepatocytes and HepG2 cells for the detection of hepatotoxic drugs in humans

    SciTech Connect (OSTI)

    Atienzar, Franck A.; Novik, Eric I.; Gerets, Helga H.; Parekh, Amit; Delatour, Claude; Cardenas, Alvaro; MacDonald, James; Yarmush, Martin L.; Dhalluin, Stphane

    2014-02-15

    Drug Induced Liver Injury (DILI) is a major cause of attrition during early and late stage drug development. Consequently, there is a need to develop better in vitro primary hepatocyte models from different species for predicting hepatotoxicity in both animals and humans early in drug development. Dog is often chosen as the non-rodent species for toxicology studies. Unfortunately, dog in vitro models allowing long term cultures are not available. The objective of the present manuscript is to describe the development of a co-culture dog model for predicting hepatotoxic drugs in humans and to compare the predictivity of the canine model along with primary human hepatocytes and HepG2 cells. After rigorous optimization, the dog co-culture model displayed metabolic capacities that were maintained up to 2 weeks which indicates that such model could be also used for long term metabolism studies. Most of the human hepatotoxic drugs were detected with a sensitivity of approximately 80% (n = 40) for the three cellular models. Nevertheless, the specificity was low approximately 40% for the HepG2 cells and hepatocytes compared to 72.7% for the canine model (n = 11). Furthermore, the dog co-culture model showed a higher superiority for the classification of 5 pairs of close structural analogs with different DILI concerns in comparison to both human cellular models. Finally, the reproducibility of the canine system was also satisfactory with a coefficient of correlation of 75.2% (n = 14). Overall, the present manuscript indicates that the dog co-culture model may represent a relevant tool to perform chronic hepatotoxicity and metabolism studies. - Highlights: Importance of species differences in drug development. Relevance of dog co-culture model for metabolism and toxicology studies. Hepatotoxicity: higher predictivity of dog co-culture vs HepG2 and human hepatocytes.

  19. A validated model to predict microalgae growth in outdoor pond cultures subjected to fluctuating light intensities and water temperatures

    SciTech Connect (OSTI)

    Huesemann, Michael H.; Crowe, Braden J.; Waller, Peter; Chavis, Aaron R.; Hobbs, Samuel J.; Edmundson, Scott J.; Wigmosta, Mark S.

    2015-12-11

    Here, a microalgae biomass growth model was developed for screening novel strains for their potential to exhibit high biomass productivities under nutrient-replete conditions in outdoor ponds subjected to fluctuating light intensities and water temperatures. Growth is modeled by first estimating the light attenuation by biomass according to a scatter-corrected Beer-Lambert Law, and then calculating the specific growth rate in discretized culture volume slices that receive declining light intensities due to attenuation. The model requires the following experimentally determined strain-specific input parameters: specific growth rate as a function of light intensity and temperature, biomass loss rate in the dark as a function of temperature and average light intensity during the preceding light period, and the scatter-corrected biomass light absorption coefficient. The model was successful in predicting the growth performance and biomass productivity of three different microalgae species (Chlorella sorokiniana, Nannochloropsis salina, and Picochlorum sp.) in raceway pond cultures (batch and semi-continuous) subjected to diurnal sunlight intensity and water temperature variations. Model predictions were moderately sensitive to minor deviations in input parameters. To increase the predictive power of this and other microalgae biomass growth models, a better understanding of the effects of mixing-induced rapid light dark cycles on photo-inhibition and short-term biomass losses due to dark respiration in the aphotic zone of the pond is needed.

  20. A validated model to predict microalgae growth in outdoor pond cultures subjected to fluctuating light intensities and water temperatures

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Huesemann, Michael H.; Crowe, Braden J.; Waller, Peter; Chavis, Aaron R.; Hobbs, Samuel J.; Edmundson, Scott J.; Wigmosta, Mark S.

    2015-12-11

    Here, a microalgae biomass growth model was developed for screening novel strains for their potential to exhibit high biomass productivities under nutrient-replete conditions in outdoor ponds subjected to fluctuating light intensities and water temperatures. Growth is modeled by first estimating the light attenuation by biomass according to a scatter-corrected Beer-Lambert Law, and then calculating the specific growth rate in discretized culture volume slices that receive declining light intensities due to attenuation. The model requires the following experimentally determined strain-specific input parameters: specific growth rate as a function of light intensity and temperature, biomass loss rate in the dark as amore » function of temperature and average light intensity during the preceding light period, and the scatter-corrected biomass light absorption coefficient. The model was successful in predicting the growth performance and biomass productivity of three different microalgae species (Chlorella sorokiniana, Nannochloropsis salina, and Picochlorum sp.) in raceway pond cultures (batch and semi-continuous) subjected to diurnal sunlight intensity and water temperature variations. Model predictions were moderately sensitive to minor deviations in input parameters. To increase the predictive power of this and other microalgae biomass growth models, a better understanding of the effects of mixing-induced rapid light dark cycles on photo-inhibition and short-term biomass losses due to dark respiration in the aphotic zone of the pond is needed.« less

  1. Predominant induction of kinetochore-containing micronuclei by extracts of diesel exhaust particulates in cultured human lymphocytes

    SciTech Connect (OSTI)

    Odagiri, Youichi; Uchida, Hiroyuki; Kawamura, Ken; Adachi, Shuichi; Takemoto, Kazuo ); Jian-Xin Zhang )

    1994-01-01

    The aneuploidy-inducing activity of extracts of diesel exhaust particulates from light duty (LD) and heavy duty (HD) engines was investigated in cultured peripheral blood lymphocytes of 8 healthy donors using the cytokinesis-block micronucleus test with the kinetochore labelling modification. A majority of the subjects tested showed a significant kinetochore-positive micronucleus induction after treatment with the highest dose (150 [mu]g/ml) of LD extract, although some subjects also showed induction of kinetochore-negative micronuclei. Only one subject had significantly increased numbers of kinetochore-positive micronuclei at a dose of 400 [mu]g/ml of HD extract. These results suggest that diesel extract, at least LD extract, possesses the ability to induce whole chromosome loss (aneuploidy) preferentially, although there are also chromosome breaks. 21 refs., 2 figs., 3 tabs.

  2. Differences in growth properties of endometrial cancer in three dimensional (3D) culture and 2D cell monolayer

    SciTech Connect (OSTI)

    Chitcholtan, Kenny; Asselin, Eric; Parent, Sophie; Sykes, Peter H.; Evans, John J.

    2013-01-01

    Three-dimensional (3D) in vitro models have an invaluable role in understanding the behaviour of tumour cells in a well defined microenvironment. This is because some aspects of tumour characteristics cannot be fully recapitulated in a cell monolayer (2D). In the present study, we compared growth patterns, expression of signalling molecules, and metabolism-associated proteins of endometrial cancer cell lines in 3D and 2D cell cultures. Cancer cells formed spherical structures in 3D reconstituted basement membrane (3D rBM), and the morphological appearance was cell line dependent. Cell differentiation was observed after 8 days in the 3D rBM. There was reduced proliferation, detected by less expression of PCNA in 3D rBM than in 2D cell monolayers. The addition of exogenous epidermal growth factor (EGF) to cancer cells induced phosphorylation of EGFR and Akt in both cell culture conditions. The uptake of glucose was selectively altered in the 3D rBM, but there was a lack of association with Glut-1 expression. The secretion of vascular endothelial growth factor (VEGF) and prostaglandin E{sub 2} (PGE{sub 2}) was selectively altered in 3D rBM, and it was cell line dependent. Our data demonstrated that 3D rBM as an in vitro model can influence proliferation and metabolism of endometrial cancer cell behaviour compared to 2D cell monolayer. Changes are specific to individual cell types. The use of 3D rBM is, therefore, important in the in vitro study of targeted anticancer therapies.

  3. Raman chemical imaging of the rhizosphere bacterium Pantoea sp. YR343 and its co-culture with Arabidopsis thaliana

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Polisetti, Sneha; Bible, Amber N.; Morrell-Falvey, Jennifer L.; Bohn, Paul W.

    2016-02-29

    Chemical imaging of plant-bacteria co-cultures renders it possible to characterize bacterial populations and behaviors and their interactions with proximal organisms, under conditions closest to the environment in the rhizosphere. Here Raman micro-spectroscopy and confocal Raman imaging are used as minimally invasive probes to study the rhizosphere bacterial isolate, Pantoea sp. YR343, and its co-culture with model plant Arabidopsis thaliana by combining enhanced Raman spectroscopies with electron microscopy and principal component analysis (PCA). The presence of carotenoid pigments in the wild type Pantoea sp. YR343 was characterized using resonance Raman scattering, which was also used to confirm successful disruption of themore » crtB gene in an engineered carotenoid mutant strain. Other components of the Pantoea sp. YR343 cells were imaged in the presence of resonantly enhanced pigments using a combination of surface enhanced Raman imaging and PCA. Pantoea sp. YR343 cells decorated with Ag colloid synthesized ex situ gave spectra dominated by carotenoid scattering, whereas colloids synthesized in situ produced spectral signatures characteristic of flavins in the cell membrane. Scanning electron microscopy (SEM) of whole cells and transmission electron microscopy (TEM) images of thinly sliced cross-sections were used to assess structural integrity of the coated cells and to establish the origin of spectral signatures based on the position of Ag nanoparticles in the cells. Finally, raman imaging was also used to characterize senescent green Arabidopsis thaliana plant roots inoculated with Pantoea sp. YR343, and PCA was used to distinguish spectral contributions from plant and bacterial cells, thereby establishing the potential of Raman imaging to visualize the distribution of rhizobacteria on plant roots.« less

  4. Characterization and response of newly developed high-grade glioma cultures to the tyrosine kinase inhibitors, erlotinib, gefitinib and imatinib

    SciTech Connect (OSTI)

    Kinsella, Paula; Howley, Rachel; Doolan, Padraig; Clarke, Colin; Madden, Stephen F.; Clynes, Martin; Farrell, Michael; Amberger-Murphy, Verena; All Ireland Co-operative, Oncology Research Group, 60 Fitzwilliam Square, Dublin 2

    2012-03-10

    High-grade gliomas (HGG), are the most common aggressive brain tumours in adults. Inhibitors targeting growth factor signalling pathways in glioma have shown a low clinical response rate. To accurately evaluate response to targeted therapies further in vitro studies are necessary. Growth factor pathway expression using epidermal growth factor receptor (EGFR), mutant EGFR (EGFRvIII), platelet derived growth factor receptor (PDGFR), C-Kit and C-Abl together with phosphatase and tensin homolog (PTEN) expression and downstream activation of AKT and phosphorylated ribosomal protein S6 (P70S6K) was analysed in 26 primary glioma cultures treated with the tyrosine kinase inhibitors (TKIs) erlotinib, gefitinib and imatinib. Response to TKIs was assessed using 50% inhibitory concentrations (IC{sub 50}). Response for each culture was compared with the EGFR/PDGFR immunocytochemical pathway profile using hierarchical cluster analysis (HCA) and principal component analysis (PCA). Erlotinib response was not strongly associated with high expression of the growth factor pathway components. PTEN expression did not correlate with response to any of the three TKIs. Increased EGFR expression was associated with gefitinib response; increased PDGFR-{alpha} expression was associated with imatinib response. The results of this in vitro study suggest gefitinib and imatinib may have therapeutic potential in HGG tumours with a corresponding growth factor receptor expression profile. -- Highlights: Black-Right-Pointing-Pointer Non-responders had low EGFR expression, high PDGFR-{beta}, and a low proliferation rate. Black-Right-Pointing-Pointer PTEN is not indicative of response to a TKI. Black-Right-Pointing-Pointer Erlotinib response was not associated with expression of the proteins examined. Black-Right-Pointing-Pointer Imatinib-response correlated with expression of PDGFR-{alpha}. Black-Right-Pointing-Pointer Gefitinib response correlated with increased expression of EGFR.

  5. Di(2-ethylhexyl) phthalate inhibits antral follicle growth, induces atresia, and inhibits steroid hormone production in cultured mouse antral follicles

    SciTech Connect (OSTI)

    Hannon, Patrick R. Brannick, Katherine E. Wang, Wei Gupta, Rupesh K. Flaws, Jodi A.

    2015-04-01

    Di(2-ethylhexyl) phthalate (DEHP) is a ubiquitous environmental toxicant found in consumer products that causes ovarian toxicity. Antral follicles are the functional ovarian units and must undergo growth, survival from atresia, and proper regulation of steroidogenesis to ovulate and produce hormones. Previous studies have determined that DEHP inhibits antral follicle growth and decreases estradiol levels in vitro; however, the mechanism by which DEHP elicits these effects is unknown. The present study tested the hypothesis that DEHP directly alters regulators of the cell cycle, apoptosis, and steroidogenesis to inhibit antral follicle functionality. Antral follicles from adult CD-1 mice were cultured with vehicle control or DEHP (1–100 μg/ml) for 24–96 h to establish the temporal effects of DEHP on the follicle. Following 24–96 h of culture, antral follicles were subjected to gene expression analysis, and media were subjected to measurements of hormone levels. DEHP increased the mRNA levels of cyclin D2, cyclin dependent kinase 4, cyclin E1, cyclin A2, and cyclin B1 and decreased the levels of cyclin-dependent kinase inhibitor 1A prior to growth inhibition. Additionally, DEHP increased the mRNA levels of BCL2-associated agonist of cell death, BCL2-associated X protein, BCL2-related ovarian killer protein, B-cell leukemia/lymphoma 2, and Bcl2-like 10, leading to an increase in atresia. Further, DEHP decreased the levels of progesterone, androstenedione, and testosterone prior to the decrease in estradiol levels, with decreased mRNA levels of side-chain cleavage, 17α-hydroxylase-17,20-desmolase, 17β-hydroxysteroid dehydrogenase, and aromatase. Collectively, DEHP directly alters antral follicle functionality by inhibiting growth, inducing atresia, and inhibiting steroidogenesis. - Highlights: • DEHP inhibits antral follicle growth by dysregulating cell cycle regulators. • DEHP induces antral follicle atresia by dysregulating apoptosis regulators. • DEHP

  6. Molecular Dissection of Induced Platinum Resistance through Functional and Gene Expression Analysis in a Cell Culture Model of Bladder Cancer

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wang, Sisi; Zhang, Hongyong; Scharadin, Tiffany M.; Zimmermann, Maike; Hu, Bin; Pan, Amy Wang; Vinall, Ruth; Lin, Tzu-yin; Cimino, George; Chain, Patrick; et al

    2016-01-22

    Here, we report the development, functional and molecular characterization of an isogenic, paired bladder cancer cell culture model system for studying platinum drug resistance. The 5637 human bladder cancer cell line was cultured over ten months with stepwise increases in oxaliplatin concentration to generate a drug resistant 5637R sub cell line. The MTT assay was used to measure the cytotoxicity of several bladder cancer drugs. Liquid scintillation counting allowed quantification of cellular drug uptake and efflux of radiolabeled oxaliplatin and carboplatin. The impact of intracellular drug inactivation was assessed by chemical modulation of glutathione levels. Oxaliplatin- and carboplatin-DNA adduct formationmore » and repair was measured using accelerator mass spectrometry. Resistance factors including apoptosis, growth factor signaling and others were assessed with RNAseq of both cell lines and included confirmation of selected transcripts by RT-PCR. Oxaliplatin, carboplatin, cisplatin and gemcitabine were significantly less cytotoxic to 5637R cells compared to the 5637 cells. In contrast, doxorubicin, methotrexate and vinblastine had no cell line dependent difference in cytotoxicity. Upon exposure to therapeutically relevant doses of oxaliplatin, 5637R cells had lower drug-DNA adduct levels than 5637 cells. This difference was partially accounted for by pre-DNA damage mechanisms such as drug uptake and intracellular inactivation by glutathione, as well as faster oxaliplatin-DNA adduct repair. In contrast, both cell lines had no significant differences in carboplatin cell uptake, efflux and drug-DNA adduct formation and repair, suggesting distinct resistance mechanisms for these two closely related drugs. The functional studies were augmented by RNAseq analysis, which demonstrated a significant change in expression of 83 transcripts, including 50 known genes and 22 novel transcripts. Most of the transcripts were not previously associated with bladder cancer

  7. A long-term three dimensional liver co-culture system for improved prediction of clinically relevant drug-induced hepatotoxicity

    SciTech Connect (OSTI)

    Kostadinova, Radina; Boess, Franziska; Suter, Laura; Weiser, Thomas; Singer, Thomas; Roth, Adrian

    2013-04-01

    Drug-induced liver injury (DILI) is the major cause for liver failure and post-marketing drug withdrawals. Due to species-specific differences in hepatocellular function, animal experiments to assess potential liabilities of drug candidates can predict hepatotoxicity in humans only to a certain extent. In addition to animal experimentation, primary hepatocytes from rat or human are widely used for pre-clinical safety assessment. However, as many toxic responses in vivo are mediated by a complex interplay among different cell types and often require chronic drug exposures, the predictive performance of hepatocytes is very limited. Here, we established and characterized human and rat in vitro three-dimensional (3D) liver co-culture systems containing primary parenchymal and non-parenchymal hepatic cells. Our data demonstrate that cells cultured on a 3D scaffold have a preserved composition of hepatocytes, stellate, Kupffer and endothelial cells and maintain liver function for up to 3 months, as measured by the production of albumin, fibrinogen, transferrin and urea. Additionally, 3D liver co-cultures maintain cytochrome P450 inducibility, form bile canaliculi-like structures and respond to inflammatory stimuli. Upon incubation with selected hepatotoxicants including drugs which have been shown to induce idiosyncratic toxicity, we demonstrated that this model better detected in vivo drug-induced toxicity, including species-specific drug effects, when compared to monolayer hepatocyte cultures. In conclusion, our results underline the importance of more complex and long lasting in vitro cell culture models that contain all liver cell types and allow repeated drug-treatments for detection of in vivo-relevant adverse drug effects. - Highlights: ? 3D liver co-cultures maintain liver specific functions for up to three months. ? Activities of Cytochrome P450s remain drug- inducible accross three months. ? 3D liver co-cultures recapitulate drug-induced liver toxicity observed

  8. An Independent Evaluation of Safety Culture at the U.S. Department of Energy Office of Health, Safety and Security– Headquarters

    Broader source: Energy.gov [DOE]

    This report describes the results of an independent evaluation of the existing safety culture at the U.S. Department of Energy Office of Health, Safety, and Security (HSS). This evaluation was conducted at the request of the Department’s Chief Health, Safety and Security Officer.

  9. Annual report on the U.S. Department of Energy`s cultural resource activities at Colorado UMTRA Project sites for October 1995--September 1996

    SciTech Connect (OSTI)

    1996-09-01

    This report summarizes the results of cultural resource activities conducted by the U.S. Department of Energy (DOE) at Uranium Mill Tailings Remedial Action (UMTRA) Project sites in Colorado for the period of October 1, 1995 through September 30, 1996. The inactive uranium mill tailings sites in Colorado are at Durango, Grand Junction, Gunnison, Maybell, Naturita, Rifle, and Slick Rock. On December 6, 1984, the DOE, the Advisory Council on Historic Preservation, and the Colorado State Historic Preservation Officer (SHPO) entered into a programmatic memorandum of understanding (PMOU). This PMOU requires the DOE to fulfillment of its obligations under various state and federal regulations for the protection and preservation of cultural resources. This report provides the state of Colorado with an annual report on the cultural resource activities performed for all UMTRA Project sites in Colorado. Due to the completion of surface activities at the UMTRA Project sites, this will be the last annual report to the state of Colorado. Cultural resources activities subsequent to this report will be reported to the state through site-specific correspondence.

  10. Induction of stable p53 oncoprotein and of c-myc overexpression in cultured normal human uroepithelium by radiation and N-nitrosodiethanolamine

    SciTech Connect (OSTI)

    Mothersill, C.; Seymour, C.B. ); Harney, J. )

    1994-04-01

    Uroepithelium cultured from normal patients without cancer (60 individuals) was found to segregate into four subtypes based on the level of carcinogen treatment needed to induce abnormal p53 and c-myc. Twenty-two percent of patient cultures never showed abnormal p53 expression, even after chronic exposure to nitrosamines, while a further 26% required only a single dose of radiation to induce the abnormal protein. The remaining patients had tissues which, while initially negative for stable p53, became positive when put into culture and stimulated to grow. The c-myc protein was overexpressed in all cultures with abnormal p53. It would appear that elevated expression of conformationally inactive p53 and of high levels of c-myc represents an early response of normal uroepithelial cells to carcinogen challenge. It also appears that a relatively high number of patients without cancer express these proteins when their cells are challenged to grow; a pre-exposure to environmental carcinogens such as nitrosamines in cigarette smoke is likely to be involved. 30 refs., 3 figs., 3 tabs.

  11. Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Therien, Jesse B.; Zadvornyy, Oleg A.; Posewitz, Matthew C.; Bryant, Donald A.; Peters, John W.

    2014-10-18

    The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. We demonstrate the growth of C. reinhardtii on acetate provided by mutant strains of the cyanobacterium Synechococcus sp. PCC7002.

  12. The US Geological Survey-Bureau of Land Management cultural resources program in the National Petroleum Reserve in Alaska, 1977-1981

    SciTech Connect (OSTI)

    Hall, E.S. Jr.; Gal, R.

    1989-01-01

    Utilization of northern Alaska's riches long predates the recent oil exploration program in the National Petroleum Reserve in Alaska (NPRA). Though the earliest known archaeological site in the reserve dates back only 7600 yr, most archaeologists believe human groups first occupied the area at least 4000 yr earlier. The as-yet-undiscovered physical remains left behind by these first inhabitants of the area, as well as the known and unknown traces of the peoples who succeeded them through time, constitute the cultural resources of the NPRA. First among the laws protecting cultural resources is the Antiquities Act of 1906, which provides for the establishment of national monuments by Presidential proclamation, sets up a permit system for the scientific investigation of cultural resources on Federal land, and details penalities for unauthorized disturbance of archaeological remains. The Archaeological and Historic Preservation Act of 1974, which extended the earlier Reservoir Salvage Act of 1960, authorizes funds for the preservation of historical and archaeological data that otherwise might be lost through any Federal construction project or federally licensed or assisted activity or program. The National Historic Preservation Act of 1966 established the National Register of Historic Places and a National Advisory Council to assist all Federal agencies in evaluating the effects of their actions on properties included, or eligible for inclusion, in the National Register. Finally, Executive Order 11593 of May 13, 1971, requires all Federal agencies to inventory cultural resources on lands they manage or affect in order to determine eligibility for the National Register, and to use due caution in regard to those resources until the inventory, evaluation, and nomination processes are completed. The oil exploration program in the NPRA is subject to this body of law for cultural resource protection.

  13. Changes in Cell Wall Properties Coincide with Overexpression of Extensin Fusion Proteins in Suspension Cultured Tobacco Cells

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Tan, Li; Pu, Yunqiao; Pattathil, Sivakumar; Avci, Utku; Qian, Jin; Arter, Allison; Chen, Liwei; Hahn, Michael G.; Ragauskas, Arthur J.; Kieliszewski, Marcia J.

    2014-12-23

    Extensins are one subfamily of the cell wall hydroxyproline-rich glycoproteins, containing characteristic SerHyp4 glycosylation motifs and intermolecular cross-linking motifs such as the TyrXaaTyr sequence. Extensins are believed to form a cross-linked network in the plant cell wall through the tyrosine-derivatives isodityrosine, pulcherosine, and di-isodityrosine. Overexpression of three synthetic genes encoding different elastin-arabinogalactan protein-extensin hybrids in tobacco suspension cultured cells yielded novel cross-linking glycoproteins that shared features of the extensins, arabinogalactan proteins and elastin. The cell wall properties of the three transgenic cell lines were all changed, but in different ways. One transgenic cell line showed decreased cellulose crystallinity and increasedmore » wall xyloglucan content; the second transgenic cell line contained dramatically increased hydration capacity and notably increased cell wall biomass, increased di-isodityrosine, and increased protein content; the third transgenic cell line displayed wall phenotypes similar to wild type cells, except changed xyloglucan epitope extractability. In conclusion, these data indicate that overexpression of modified extensins may be a route to engineer plants for bioenergy and biomaterial production.« less

  14. Changes in Cell Wall Properties Coincide with Overexpression of Extensin Fusion Proteins in Suspension Cultured Tobacco Cells

    SciTech Connect (OSTI)

    Tan, Li; Pu, Yunqiao; Pattathil, Sivakumar; Avci, Utku; Qian, Jin; Arter, Allison; Chen, Liwei; Hahn, Michael G.; Ragauskas, Arthur J.; Kieliszewski, Marcia J.

    2014-12-23

    Extensins are one subfamily of the cell wall hydroxyproline-rich glycoproteins, containing characteristic SerHyp4 glycosylation motifs and intermolecular cross-linking motifs such as the TyrXaaTyr sequence. Extensins are believed to form a cross-linked network in the plant cell wall through the tyrosine-derivatives isodityrosine, pulcherosine, and di-isodityrosine. Overexpression of three synthetic genes encoding different elastin-arabinogalactan protein-extensin hybrids in tobacco suspension cultured cells yielded novel cross-linking glycoproteins that shared features of the extensins, arabinogalactan proteins and elastin. The cell wall properties of the three transgenic cell lines were all changed, but in different ways. One transgenic cell line showed decreased cellulose crystallinity and increased wall xyloglucan content; the second transgenic cell line contained dramatically increased hydration capacity and notably increased cell wall biomass, increased di-isodityrosine, and increased protein content; the third transgenic cell line displayed wall phenotypes similar to wild type cells, except changed xyloglucan epitope extractability. In conclusion, these data indicate that overexpression of modified extensins may be a route to engineer plants for bioenergy and biomaterial production.

  15. High density culture of white bass X striped bass fingerlings in raceways using power plant heated effluent

    SciTech Connect (OSTI)

    Collins, C.M.; Burton, G.L.; Schweinforth, R.L.

    1983-06-01

    White bass (Morone chrysops) X striped bass (M. saxatilis) hybrids weighing 1691/lb were initially stocked in five 24 ft/sup 3/ floating screen cages for 20 days. Hybrids averaging one inch in total length and 361 fish/lb were released in four 614 ft/sup 3/ concrete raceways. Two stocking densities, 2.6 and 5.1 fish/ft/sup 3/, were evaluated in the 94-day study using a flow rate of 300 gpm/raceway. Water temperatures averaged 79/sup 0/F and water quality was adequate throughout the production period. Fish were hand fed to satiation daily. Columnaris and Aeromonas hydrophila caused the most serious disease problems. Gas supersaturation was suspect in high mortality levels during cage culture of hybrid bass fry. Cannibalism may have been responsible for unaccountable losses prior to raceway stocking and at harvest. The study yielded 5773 hybrids weighing 658 lb. The high density treatment showed greater weight gain, average weight, average length and percent survival as well as improved food conversion. Results suggest that higher stocking densities and periodic grading may increase production and suppress cannibalism. 10 references, 3 figures, 3 tables.

  16. Calculating the Cultural Significance...

    National Nuclear Security Administration (NNSA)

    ... In the latter region, Nancy Turner and colleagues have made extensive contributions (Turner 1973, 1974, 1975, 1978, 1979, 1987, 1988a; Turner and Bell 1971, 1973; Turner, Bouchard, ...

  17. Cell culture compositions

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel; Goedegebuur, Frits; Ward, Michael; Yiao, Jian

    2014-03-18

    The present invention provides a novel endoglucanase nucleic acid sequence, designated egl6 (SEQ ID NO:1 encodes the full length endoglucanase; SEQ ID NO:4 encodes the mature form), and the corresponding endoglucanase VI amino acid sequence ("EGVI"; SEQ ID NO:3 is the signal sequence; SEQ ID NO:2 is the mature sequence). The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVI, recombinant EGVI proteins and methods for producing the same.

  18. Semi-synthetic preparation of 1-O-(1'-/sup 14/C)hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor) using plant cell cultures

    SciTech Connect (OSTI)

    Weber, N.; Mangold, H.K.

    1985-04-01

    Incubation of photomixotrophic cell suspension cultures of rape (Brassica napus) and heterotrophic cell suspension cultures of soya (Glycine max) with 1-O-(1'-/sup 14/C)hexadecyl-sn-glycerol or rac-1-O-(1'-/sup 14/C)hexadecylglycerol leads in high yield (up to 78%) to labeled 1-O-hexadecyl-2-acyl-sn-glycero-3-phosphocholines. Alkaline hydrolysis of the choline glycerophospholipids yields pure 1-O-(1'-/sup 14/C)hexadecyl-sn-glycero-3-phosphocholine. 1-O-(1'-14C)Hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor) is obtained by acetylating the lyso compound. The semi-synthetic preparation described leads to labeled platelet activating factor in an overall yield of 50-60% without loss of specific activity.

  19. Independent Oversight Assessment of the Nuclear Safety Culture and Management of Nuclear Safety Concerns at the Hanford Site Waste Treatment and Immobilization Plant, January 2012

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Health, Safety and Security HSS Independent Oversight Assessment of Nuclear Safety Culture and Management of Nuclear Safety Concerns at the Hanford Site Waste Treatment and Immobilization Plant January 2012 Office of Enforcement and Oversight Office of Health, Safety and Security U.S. Department of Energy Enforcement and Oversight Abbreviations Used in this Report i Executive Summary iii Recommendations xi 1.0 Introduction 1 1.1 Background 2 1.2 Scope and Methodology 6 2.0 Current Safety

  20. A Cultural Resources Inventory and Historical Evaluation of the Smoky Atmospheric Nuclear Test, Areas 8, 9, and 10, Nevada National Security Site, Nye County, Nevada

    SciTech Connect (OSTI)

    Jones, Robert C.; King, Maureen L.; Beck, Colleen M.; Falvey, Lauren W.; Menocal, Tatianna M.

    2014-09-01

    This report presents the results of a National Historic Preservation Act Section 106 cultural resources inventory and historical evaluation of the 1957 Smoky atmospheric test location on the Nevada National Security Site (NNSS). The Desert Research Institute (DRI) was tasked to conduct a cultural resources study of the Smoky test area as a result of a proposed undertaking by the Department of Energy Environmental Management. This undertaking involves investigating Corrective Action Unit (CAU) 550 for potential contaminants of concern as delineated in a Corrective Action Investigation Plan. CAU 550 is an area that spatially overlaps portions of the Smoky test location. Smoky, T-2c, was a 44 kt atmospheric nuclear test detonated at 5:30 am on August 31, 1957, on top of a 213.4 m (700 ft) 200 ton tower (T-2c) in Area 8 of the NNSS. Smoky was a weapons related test of the Plumbbob series (number 19) and part of the Department of Defense Exercise Desert Rock VII and VIII. The cultural resources effort involved the development of a historic context based on archival documents and engineering records, the inventory of the cultural resources in the Smoky test area and an associated military trench location in Areas 9 and 10, and an evaluation of the National Register eligibility of the cultural resources. The inventory of the Smoky test area resulted in the identification of structures, features, and artifacts related to the physical development of the test location and the post-test remains. The Smoky test area was designated historic district D104 and coincides with a historic archaeological site recorded as 26NY14794 and the military trenches designed for troop observation, site 26NY14795. Sites 26NY14794 and 26NY14795 are spatially discrete with the trenches located 4.3 km (2.7 mi) southeast of the Smoky ground zero. As a result, historic district D104 is discontiguous and in total it covers 151.4 hectares (374 acres). The Smoky test location, recorded as historic

  1. Thyroid organotypic rat and human cultures used to investigate drug effects on thyroid function, hormone synthesis and release pathways

    SciTech Connect (OSTI)

    Vickers, Alison E.M.; Heale, Jason; Sinclair, John R.; Morris, Stephen; Rowe, Josh M.; Fisher, Robyn L.

    2012-04-01

    Drug induced thyroid effects were evaluated in organotypic models utilizing either a rat thyroid lobe or human thyroid slices to compare rodent and human response. An inhibition of thyroid peroxidase (TPO) function led to a perturbation in the expression of key genes in thyroid hormone synthesis and release pathways. The clinically used thiourea drugs, methimazole (MMI) and 6-n-propyl-2-thioruacil (PTU), were used to evaluate thyroid drug response in these models. Inhibition of TPO occurred early as shown in rat thyroid lobes (2 h) and was sustained in both rat (24–48 h) and human (24 h) with ≥ 10 μM MMI. Thyroid from rats treated with single doses of MMI (30–1000 mg/kg) exhibited sustained TPO inhibition at 48 h. The MMI in vivo thyroid concentrations were comparable to the culture concentrations (∼ 15–84 μM), thus demonstrating a close correlation between in vivo and ex vivo thyroid effects. A compensatory response to TPO inhibition was demonstrated in the rat thyroid lobe with significant up-regulation of genes involved in the pathway of thyroid hormone synthesis (Tpo, Dio1, Slc5a5, Tg, Tshr) and the megalin release pathway (Lrp2) by 24 h with MMI (≥ 10 μM) and PTU (100 μM). Similarly, thyroid from the rat in vivo study exhibited an up-regulation of Dio1, Slc5a5, Lrp2, and Tshr. In human thyroid slices, there were few gene expression changes (Slc5a5, ∼ 2-fold) and only at higher MMI concentrations (≥ 1500 μM, 24 h). Extended exposure (48 h) resulted in up-regulation of Tpo, Dio1 and Lrp2, along with Slc5a5 and Tshr. In summary, TPO was inhibited by similar MMI concentrations in rat and human tissue, however an increased sensitivity to drug treatment in rat is indicated by the up-regulation of thyroid hormone synthesis and release gene pathways at concentrations found not to affect human tissue. -- Highlights: ► Novel model of rat thyroid or human thyroid slices to evaluate pathways of injury. ► TPO inhibition by MMI or PTU altered

  2. Bioenergy systems report: The AID (Agency for International Development) approach. Using agricultural and forestry wastes for the production of energy in support of rural development

    SciTech Connect (OSTI)

    Not Available

    1989-04-01

    The Biomass Energy Systems and Technology project (BEST) seeks to integrate natural resources, private sector expertise, and financial support in order to convert biomass into marketable energy products at existing agro-processing facilities. This report documents BEST's approach to biomass promotion and includes sections on: the rationale for the project's commodity focus (sugar cane, rice, and wood); the relevant U.S. biomass experience with rice, cane, and wood residues, etc., which BEST draws upon; A.I.D.'s experience in the field application of rice, wood, and cane residue bioenergy systems; economic analyses of biomass systems (using examples from Indonesia and Costa Rica); research initiatives to develop off-season fuels for sugar mills, advanced biomass conversion systems, and energy efficiency in sugar factories; and the environmental aspects of biomass (including its ability to be used without increasing global warming).

  3. Tennessee's 5th congressional district: Energy Resources | Open...

    Open Energy Info (EERE)

    Tennessee's 5th congressional district Agri Energy Inc Nashville Electric Service NES Universal Lighting Technologies Retrieved from "http:en.openei.orgwindex.php?titleTenness...

  4. Davidson County, Tennessee: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    in Davidson County, Tennessee Agri Energy Inc Nashville Electric Service NES Universal Lighting Technologies Energy Generation Facilities in Davidson County, Tennessee MM...

  5. Nashville, Tennessee: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Companies in Nashville, Tennessee Agri Energy Inc Nashville Electric Service NES Universal Lighting Technologies References US Census Bureau Incorporated place and minor...

  6. Christian County, Kentucky: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Commonwealth AgriEnergy Places in Christian County, Kentucky Crofton, Kentucky Fort Campbell North, Kentucky Hopkinsville, Kentucky LaFayette, Kentucky Oak Grove, Kentucky...

  7. Nebraska's 3rd congressional district: Energy Resources | Open...

    Open Energy Info (EERE)

    Nebraska. Registered Energy Companies in Nebraska's 3rd congressional district Chief Ethanol Fuels Chief Ethanol Fuels Inc Husker Ag LLC Kaapa Ethanol LLC Mid America Agri...

  8. Tennessee's 7th congressional district: Energy Resources | Open...

    Open Energy Info (EERE)

    Companies in Tennessee's 7th congressional district Agri Energy Inc Biofuels America Inc Eco Energy Inc Memphis Biofuels LLC Nashville Electric Service NES Ocean Motion...

  9. West Central Cooperative | Open Energy Information

    Open Energy Info (EERE)

    Cooperative Jump to: navigation, search Name: West Central Cooperative Place: Ralston, Iowa Zip: 51459 Product: Iowa-based agri-business with operations across the United States....

  10. Escambia County, Florida: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    County, Florida Agri Source Fuels Places in Escambia County, Florida Bellview, Florida Brent, Florida Century, Florida Ensley, Florida Ferry Pass, Florida Gonzalez, Florida...

  11. Renville County, Minnesota: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Falls Energy Minnesota Energy Sunrise Agri Fuels Places in Renville County, Minnesota Bird Island, Minnesota Buffalo Lake, Minnesota Danube, Minnesota Fairfax, Minnesota...

  12. STTR Phase 1 Final Technical Report for Project Entitled "Developing...

    Office of Scientific and Technical Information (OSTI)

    The goal of this project, sponsored by Agri-Tech Producers, LLC (ATP), the small business ... Country of Publication: United States Language: English Subject: 09 BIOMASS FUELS ...

  13. Colorado's 5th congressional district: Energy Resources | Open...

    Open Energy Info (EERE)

    Energy Companies in Colorado's 5th congressional district AC Solar Inc American Agri diesel LLC American Electric Vehicles Inc American Solar Technology BBI International Diamond...

  14. El Paso County, Colorado: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Zone Subtype B. Registered Energy Companies in El Paso County, Colorado American Agri diesel LLC American Electric Vehicles Inc American Solar Technology Diamond Wire Technology...

  15. Colorado Springs, Colorado: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Registered Energy Companies in Colorado Springs, Colorado American Agri diesel LLC American Solar Technology Diamond Wire Technology LLC Larankelo Mobile Energy...

  16. Minnesota's 1st congressional district: Energy Resources | Open...

    Open Energy Info (EERE)

    1st congressional district Agra Resources Cooperative EXOL Agri Energy LLC Corn Plus High Country Energy Juhl Wind Inc MinnErgy LLC Minwind Energy LLC Next Generation...

  17. Kansas's 1st congressional district: Energy Resources | Open...

    Open Energy Info (EERE)

    district Conestoga Energy Partners LLC ESE Alcohol Gateway Ethanol LLC formerly Wildcat Bio Energy LLC Kansas Ethanol LLC Nesika Energy LLC Orion Ethanol Reeve Agri Energy Inc...

  18. Mono-hydroxy methoxychlor alters levels of key sex steroids and steroidogenic enzymes in cultured mouse antral follicles

    SciTech Connect (OSTI)

    Craig, Zelieann R.; Leslie, Traci C.; Hatfield, Kimberly P.; Gupta, Rupesh K.; Flaws, Jodi A.

    2010-12-01

    Methoxychlor (MXC) is an organochlorine pesticide that reduces fertility in female rodents by decreasing antral follicle numbers and increasing follicular death. MXC is metabolized in the body to mono-hydroxy MXC (mono-OH). Little is known about the effects of mono-OH on the ovary. Thus, this work tested the hypothesis that mono-OH exposure decreases production of 17{beta}-estradiol (E{sub 2}) by cultured mouse antral follicles. Antral follicles were isolated from CD-1 mice (age 35-39 days) and exposed to dimethylsulfoxide (DMSO), or mono-OH (0.1-10 {mu}g/mL) for 96 h. Media and follicles were collected for analysis of sex steroid levels and mRNA expression, respectively. Mono-OH treatment (10 {mu}g/mL) decreased E{sub 2} (DMSO: 3009.72 {+-} 744.99 ng/mL; mono-OH 0.1 {mu}g/mL: 1679.66 {+-} 461.99 ng/mL; 1 {mu}g/mL: 1752.72 {+-} 532.41 ng/mL; 10 {mu}g/mL: 45.89 {+-} 33.83 ng/mL), testosterone (DMSO: 15.43 {+-} 2.86 ng/mL; mono-OH 0.1 {mu}g/mL: 17.17 {+-} 4.71 ng/mL; 1 {mu}g/mL: 13.64 {+-} 3.53 ng/mL; 10 {mu}g/mL: 1.29 {+-} 0.23 ng/mL), androstenedione (DMSO: 1.92 {+-} 0.34 ng/mL; mono-OH 0.1 {mu}g/mL: 1.49 {+-} 0.43 ng/mL; 1 {mu}g/mL: 0.64 {+-} 0.31 ng/mL; 10 {mu}g/mL: 0.12 {+-} 0.06 ng/mL) and progesterone (DMSO: 24.11 {+-} 4.21 ng/mL; mono-OH 0.1 {mu}g/mL: 26.77 {+-} 4.41 ng/mL; 1 {mu}g/mL: 20.90 {+-} 3.75 ng/mL; 10 {mu}g/mL: 9.44 {+-} 2.97 ng/mL) levels. Mono-OH did not alter expression of Star, Hsd3b1, Hsd17b1 and Cyp1b1, but it did reduce levels of Cyp11a1, Cyp17a1 and Cyp19a1 mRNA. Collectively, these data suggest that mono-OH significantly decreases levels of key sex steroid hormones and the expression of enzymes required for steroidogenesis.

  19. CIFOR/ICRAF Forests and Climate Training | Open Energy Information

    Open Energy Info (EERE)

    Company Organization: United States Agency for International Development, Center for International Forestry Research Sector: Land Focus Area: - Biofuels, Forestry, Offsets and...

  20. Gateway:Amrica Latina/Aprender ms sobre las ERNC/Seleccion...

    Open Energy Info (EERE)

    Fuente: Forestry Webinar Portal Idioma: Ingls The South Rises Again: Industrial Forest Management in Chile Fuente: Forestry Webinar Portal Idioma: Ingls Residuos Orgnicos An...

  1. Climate Change Mitigation Through Land-Use Measures in the Agriculture...

    Open Energy Info (EERE)

    and Forestry Sectors Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Climate Change Mitigation Through Land-Use Measures in the Agriculture and Forestry...

  2. Utility of four strains of white-rot fungi for the detoxification of 2,4,6-trinitrotoluene in liquid culture

    SciTech Connect (OSTI)

    Donnelly, K.C.; Chen, J.C.; Huebner, H.J.; Brown, K.W.; Autenrieth, R.L.; Bonner, J.S.

    1997-06-01

    The purpose of this study was to investigate the potential of four different strains of white-rot fungi (Phanerochaete chrysosporium, Phanerochaete sordida, Phlebia brevispora, and Cyathus stercoreus) to degrade 2,4,6-trinitrotoluene (TNT) in liquid medium. Loss of TNT from the culture medium was determined using high-performance liquid chromatography (HPLC), while the mutagenicity of the medium residues were evaluated using the Salmonella/microsome bioassay. The data indicate that within 21 d of incubation, all fungi were able to reduce the TNT concentration in the liquid medium to below detection limits. In this study, P. sordida showed a relatively high growth rate and the fastest rate of TNT degradation. The fungal treatment also produced a significant reduction of TNT mutagenicity. Treatment with C. stercoreus, P. brevispora, P. sordida, and P. chrysosporium resulted in the elimination of 94%, 90%, 87%, and 67% of the initial TNT-amended medium mutagenicity, respectively. The data also demonstrate that during incubation, TNT was eliminated from the culture medium two to eight times faster than the reduction in mutagenic potential. These results suggest that TNT disappearance alone cannot be used as the sole criterion in TNT remediation. Chemical analysis revealed that the major metabolites in the initial transformation of TNT were the monoamino-dinitrotoluenes, which were also degraded by the selected white-rot fungi. The study demonstrated that the white-rot fungi are capable of metabolizing and detoxifying TNT under aerobic conditions in nonligninolytic liquid medium.

  3. Effect of long-term culturing on the potential of mouse embryonic stem cells for in vitro and in vivo development

    SciTech Connect (OSTI)

    Mitalipov, Sh.M.; Mitalipova, M.M.; Ivanov, V.I.

    1994-11-01

    We performed comparative analysis of in vitro and in vivo pluripotency for two clones of ES-D3 cells subjected to different number of passages after the beginning of subcloning. Both clones of ES cells produced characteristically shaped colonies and embryoid bodies during culturing in suspension. High activity of alkaline phosphatase was demonstrated in ES cells by cytochemical staining. The proportion of aneuploid ES cells increased with the increase in the number of passages, as shown by karyotyping. Experiments on producing chimeric mice using ES cells have shown that clone D3W (passage 17) exceeds clone D3M (passage 42) both in terms of chimera proportion among the offspring and in terms of the extent of coat chimerism (proportion of agouti coat color (ES component) in the coat of chimeras). 26 refs., 4 figs., 3 tabs.

  4. Breast Cancer Cells in Three-dimensional Culture Display an Enhanced Radioresponse after Coordinate Targeting of Integrin ?5?1 and Fibronectin

    SciTech Connect (OSTI)

    Nam, Jin-Min; Onodera, Yasuhito; Bissell, Mina J; Park, Catherine C

    2010-04-07

    Tactics to selectively enhance cancer radioresponse are of great interest. Cancer cells actively elaborate and remodel their extracellular matrix (ECM) to aid in survival and progression. Previous work has shown that {beta}1-integrin inhibitory antibodies can enhance the growth-inhibitory and apoptotic responses of human breast cancer cell lines to ionizing radiation, either when cells are cultured in three-dimensional laminin-rich ECM (3D lrECM) or grown as xenografts in mice. Here, we show that a specific {alpha} heterodimer of {beta}1-integrin preferentially mediates a prosurvival signal in human breast cancer cells that can be specifically targeted for therapy. 3D lrECM culture conditions were used to compare {alpha}-integrin heterodimer expression in malignant and nonmalignant cell lines. Under these conditions, we found that expression of {alpha}5{beta}1-integrin was upregulated in malignant cells compared with nonmalignant breast cells. Similarly, we found that normal and oncofetal splice variants of fibronectin, the primary ECM ligand of {alpha}5{beta}1-integrin, were also strikingly upregulated in malignant cell lines compared with nonmalignant acini. Cell treatment with a peptide that disrupts the interactions of {alpha}5{beta}1-integrin with fibronectin promoted apoptosis in malignant cells and further heightened the apoptotic effects of radiation. In support of these results, an analysis of gene expression array data from breast cancer patients revealed an association of high levels of {alpha}5-integrin expression with decreased survival. Our findings offer preclinical validation of fibronectin and {alpha}5{beta}1-integrin as targets for breast cancer therapy.

  5. Potential role of 20S proteasome in maintaining stem cell integrity of human bone marrow stromal cells in prolonged culture expansion

    SciTech Connect (OSTI)

    Lu, Li; Song, Hui-Fang; Zhang, Wei-Guo; Liu, Xue-Qin; Zhu, Qian; Cheng, Xiao-Long; Yang, Gui-Jiao; Li, Ang; Xiao, Zhi-Cheng; Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Melbourne 3800

    2012-05-25

    Highlights: Black-Right-Pointing-Pointer Prolonged culture expansion retards proliferation and induces senescence of hBMSCs. Black-Right-Pointing-Pointer Reduced 20S proteasomal activity and expression potentially contribute to cell aging. Black-Right-Pointing-Pointer MG132-mediated 20S proteasomal inhibition induces senescence-like phenotype. Black-Right-Pointing-Pointer 18{alpha}-GA stimulates proteasomal activity and restores replicative senescence. Black-Right-Pointing-Pointer 18{alpha}-GA retains differentiation without affecting stem cell characterizations. -- Abstract: Human bone marrow stromal cells (hBMSCs) could be used in clinics as precursors of multiple cell lineages following proper induction. Such application is impeded by their characteristically short lifespan, together with the increasing loss of proliferation capability and progressive reduction of differentiation potential after the prolonged culture expansion. In the current study, we addressed the possible role of 20S proteasomes in this process. Consistent with prior reports, long-term in vitro expansion of hBMSCs decreased cell proliferation and increased replicative senescence, accompanied by reduced activity and expression of the catalytic subunits PSMB5 and PSMB1, and the 20S proteasome overall. Application of the proteasome inhibitor MG132 produced a senescence-like phenotype in early passages, whereas treating late-passage cells with 18{alpha}-glycyrrhetinic acid (18{alpha}-GA), an agonist of 20S proteasomes, delayed the senescence progress, enhancing the proliferation and recovering the capability of differentiation. The data demonstrate that activation of 20S proteasomes assists in counteracting replicative senescence of hBMSCs expanded in vitro.

  6. Blood cell oxidative stress precedes hemolysis in whole blood-liver slice co-cultures of rat, dog, and human tissues

    SciTech Connect (OSTI)

    Vickers, Alison E.M.; Sinclair, John R.; Fisher, Robyn L.; Morris, Stephen R.; Way, William

    2010-05-01

    A novel in vitro model to investigate time-dependent and concentration-dependent responses in blood cells and hemolytic events is studied for rat, dog, and human tissues. Whole blood is co-cultured with a precision-cut liver slice. Methimazole (MMI) was selected as a reference compound, since metabolism of its imidazole thione moiety is linked with hematologic disorders and hepatotoxicity. An oxidative stress response occurred in all three species, marked by a decline in blood GSH levels by 24 h that progressed, and preceded hemolysis, which occurred at high MMI concentrations in the presence of a liver slice with rat (>= 1000 muM at 48 h) and human tissues (>= 1000 muM at 48 h, >= 750 muM at 72 h) but not dog. Human blood-only cultures exhibited a decline of GSH levels but minimal to no hemolysis. The up-regulation of liver genes for heme degradation (Hmox1 and Prdx1), iron cellular transport (Slc40a1), and GSH synthesis and utilization (mGST1 and Gclc) were early markers of the oxidative stress response. The up-regulation of the Kupffer cell lectin Lgals3 gene expression indicated a response to damaged red blood cells, and Hp (haptoglobin) up-regulation is indicative of increased hemoglobin uptake. Up-regulation of liver IL-6 and IL-8 gene expression suggested an activation of an inflammatory response by liver endothelial cells. In summary, MMI exposure led to an oxidative stress response in blood cells, and an up-regulation of liver genes involved with oxidative stress and heme homeostasis, which was clearly separate and preceded frank hemolysis.

  7. The generation of 4-hydroxynonenal, an electrophilic lipid peroxidation end product, in rabbit cornea organ cultures treated with UVB light and nitrogen mustard

    SciTech Connect (OSTI)

    Zheng, Ruijin; Po, Iris; Mishin, Vladimir; Black, Adrienne T.; Heck, Diane E.; Laskin, Debra L.; Sinko, Patrick J.; Gerecke, Donald R.; Gordon, Marion K.; Laskin, Jeffrey D.

    2013-10-15

    The cornea is highly sensitive to oxidative stress, a process that can lead to lipid peroxidation. Ultraviolet light B (UVB) and nitrogen mustard (mechlorethamine) are corneal toxicants known to induce oxidative stress. Using a rabbit air-lifted corneal organ culture model, the oxidative stress responses to these toxicants in the corneal epithelium was characterized. Treatment of the cornea with UVB (0.5 J/cm{sup 2}) or nitrogen mustard (100 nmol) resulted in the generation of 4-hydroxynonenal (4-HNE), a reactive lipid peroxidation end product. This was associated with increased expression of the antioxidant, heme oxygenase-1 (HO-1). In human corneal epithelial cells in culture, addition of 4-HNE or 9-nitrooleic acid, a reactive nitrolipid formed during nitrosative stress, caused a time-dependent induction of HO-1 mRNA and protein; maximal responses were evident after 10 h with 30 μM 4-HNE or 6 h with 10 μM 9-nitrooleic acid. 4-HNE and 9-nitrooleic acid were also found to activate Erk1/2, JNK and p38 MAP kinases, as well as phosphoinositide-3-kinase (PI3)/Akt. Inhibition of p38 blocked 4-HNE- and 9-nitrooleic acid-induced HO-1 expression. Inhibition of Erk1/2, and to a lesser extent, JNK and PI3K/Akt, suppressed only 4-HNE-induced HO-1, while inhibition of JNK and PI3K/Akt, but not Erk1/2, partly reduced 9-nitrooleic acid-induced HO-1. These data indicate that the actions of 4-HNE and 9-nitrooleic acid on corneal epithelial cells are distinct. The sensitivity of corneal epithelial cells to oxidative stress may be an important mechanism mediating tissue injury induced by UVB or nitrogen mustard. - Highlights: • UVB or nitrogen mustard causes rabbit corneal epithelial injury. • 4-Hydroxynonenal (4-HNE) was formed and heme oxygenase-1 (HO-1) was increased. • 4-HNE induced HO-1 mRNA and protein expression in human corneal epithelial cells. • The induction of HO-1 by 4-HNE was through MAP kinase activation.

  8. Kruppel-like factor 2 inhibit the angiogenesis of cultured human liver sinusoidal endothelial cells through the ERK1/2 signaling pathway

    SciTech Connect (OSTI)

    Zeng, Xiao-Qing; Li, Na; Pan, Du-Yi; Miao, Qing; Ma, Gui-Fen; Liu, Yi-Mei; Tseng, Yu-Jen; Li, Feng; Xu, Li-Li; Chen, Shi-Yao

    2015-09-04

    Kruppel-like factor 2 (KLF2) is a crucial anti-angiogenic factor. However, its precise role in hepatic angiogenesis induced by liver sinusoidal endothelial cells (LSECs) remain unclear. This study was aimed to evaluate the effect of KLF2 on angiogenesis of LSECs and to explore the corresponding mechanism. Cultured human LSECs were infected with different lentiviruses to overexpress or suppress KLF2 expression. The CCK-8 assay, transwell migration assay and tube formation test, were used to investigate the roles of KLF2 in the proliferation, migration and vessel tube formation of LSECs, respectively. The expression and phosphorylation of ERK1/2 were detected by western blot. We discovered that the up-regulation of KLF2 expression dramatically inhibited proliferation, migration and tube formation in treated LSECs. Correspondingly, down-regulation of KLF2 expression significantly promoted proliferation, migration and tube formation in treated LSECs. Additionally, KLF2 inhibited the phosphorylation of ERK1/2 pathway, followed by the function of KLF2 in the angiogenesis of LSECs disrupted. In conclusion, KLF2 suppressed the angiogenesis of LSECs through inhibition of cell proliferation, migration, and vessel tube formation. These functions of KLF2 may be mediated through the ERK1/2 signaling pathway. - Highlights: • Overexpression of KLF2 inhibits the proliferation and migration of LSECs. • Overexpression of KLF2 inhibits the angiogenesis of LSECs. • ERK1/2 signaling pathway involved in the anti-angiogenic process of KLF2 on LSECs.

  9. High-quality draft genome sequence of Gracilimonas tropica CL-CB462T (DSM 19535T), isolated from a Synechococcus culture

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Choi, Dong Han; Ahn, Chisang; Jang, Gwang Il; Lapidus, Alla; Han, James; Reddy, T. B. K.; Huntemann, Marcel; Pati, Amrita; Ivanova, Natalia; Markowitz, Victor; et al

    2015-11-11

    Gracilimonas tropica Choi et al. 2009 is a member of order Sphingobacteriales, class Sphingobacteriia. Three species of the genus Gracilimonas have been isolated from marine seawater or a salt mine and showed extremely halotolerant and mesophilic features, although close relatives are extremely halophilic or thermophilic. The type strain of the type species of Gracilimonas, G. tropica DSM19535T, was isolated from a Synechococcus culture which was established from the tropical sea-surface water of the Pacific Ocean. The genome of the strain DSM19535T was sequenced through the Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes project. Here, wemore » describe the genomic features of the strain. The 3,831,242 bp long draft genome consists of 48 contigs with 3373 protein-coding and 53 RNA genes. Finally, the strain seems to adapt to phosphate limitation and requires amino acids from external environment. In addition, genomic analyses and pasteurization experiment suggested that G. tropica DSM19535T did not form spore.« less

  10. High-quality draft genome sequence of Gracilimonas tropica CL-CB462T (DSM 19535T), isolated from a Synechococcus culture

    SciTech Connect (OSTI)

    Choi, Dong Han; Ahn, Chisang; Jang, Gwang Il; Lapidus, Alla; Han, James; Reddy, T. B. K.; Huntemann, Marcel; Pati, Amrita; Ivanova, Natalia; Markowitz, Victor; Rohde, Manfred; Tindall, Brian; Göker, Markus; Woyke, Tanja; Klenk, Hans-Peter; Kyrpides, Nikos C.; Cho, Byung Cheol

    2015-11-11

    Gracilimonas tropica Choi et al. 2009 is a member of order Sphingobacteriales, class Sphingobacteriia. Three species of the genus Gracilimonas have been isolated from marine seawater or a salt mine and showed extremely halotolerant and mesophilic features, although close relatives are extremely halophilic or thermophilic. The type strain of the type species of Gracilimonas, G. tropica DSM19535T, was isolated from a Synechococcus culture which was established from the tropical sea-surface water of the Pacific Ocean. The genome of the strain DSM19535T was sequenced through the Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes project. Here, we describe the genomic features of the strain. The 3,831,242 bp long draft genome consists of 48 contigs with 3373 protein-coding and 53 RNA genes. Finally, the strain seems to adapt to phosphate limitation and requires amino acids from external environment. In addition, genomic analyses and pasteurization experiment suggested that G. tropica DSM19535T did not form spore.

  11. FermiCulture Subscription Form

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    This is a private, announcement-only mail list and will never be used for spamming or discussions. Subscribers receive approximately five emails per month. Use the form below to ...

  12. Leaders Influence Culture and Performance

    Broader source: Energy.gov [DOE]

    Presenter: Melvin G. Williams, Jr., Vice Admiral, US Navy (retired), Associate Deputy Secretary, US Department of Energy

  13. Cultural Alimentation in Latin America

    ScienceCinema (OSTI)

    None

    2011-04-25

    Le Prof. Paolo Freire(nom?) a dirigé en Brésil un plan national d'alphabétisatation d'adultes. La base de sa méthode est d'essayer de ne pas rester sur la mécanique du mot, mais de le relier avec la réalité sociale et donner un réveillement critique de la conscience populaire en face de la réalité historique du pays. Il était professeur d'histoire et de philosophie de Récife, puis exilé et depuis il était prof. à Harvard, a travaillé à l'Unesco et est maintenant conseiller spécial à l'Office d'Education du centre oecuménique des églises

  14. "The Success of Captive Broodstock Programs Depends on High In-Culture Survival, ..." [from the Abstract], 2006-2007 Progress Report.

    SciTech Connect (OSTI)

    Berejikian, Barry A.

    2009-04-08

    The success of captive broodstock programs depends on high in-culture survival, appropriate development of the reproductive system, and the behavior and survival of cultured salmon after release, either as adults or juveniles. Continuing captive broodstock research designed to improve technology is being conducted to cover all major life history stages of Pacific salmon. Accomplishments detailed in this report are listed below by major objective. Objective 1: This study documented that captively reared Chinook exhibited spawn timing similar to their founder anadromous population. An analysis of spawn timing data of captively reared Chinook salmon that had received different levels of antibiotic treatment did not suggest that antibiotic treatments during the freshwater or seawater phase of the life cycle affects final maturation timing. No effect of rearing density was found with respect to spawn timing or other reproductive behaviors. Objective 2: This study investigated the critical period(s) for imprinting for sockeye salmon by exposing juvenile salmon to known odorants at key developmental stages. Molecular assessments of imprinting-induced changes in odorant receptor gene expression indicated that regulation of odorant expression differs between coho and sockeye salmon. While temporal patterns differ between these species, exposure to arginine elicited increases in odorant receptor mRNA expression in sockeye salmon. Objective 3: This study: (i) identified the critical period when maturation is initiated in male spring Chinook salmon and when body growth affects onset of puberty, (ii) described changes in the reproductive endocrine system during onset of puberty and throughout spermatogenesis in male spring Chinook salmon, (iii) found that the rate of oocyte development prior to vitellogenesis is related to body growth in female spring Chinook, and (iv) demonstrated that growth regimes which reduce early (age 2) male maturation slow the rate of primary and early

  15. Immunosuppressive effect of the anti-IL-2-receptor monoclonal antibody, AMT-13, on organ-cultured fetal pancreas allograft survival

    SciTech Connect (OSTI)

    Burkhardt, K.; Loughnan, M.S.; Diamantstein, T.; Mandel, T.E.

    1988-11-01

    Recently, prolongation of cardiac allograft survival in mice was reported using a rat anti-IL-2R mAb (AMT-13). However, its immunosuppressive action in vivo, alone and in combination with other immunosuppressants, and its effect on other organ transplants has not been extensively studied. We grafted cultured fetal pancreas from CBA (H-2k) donors to Balb/c (H-2d) mice. Recipients were treated with 10 consecutive daily injections each of 20 micrograms AMT-13 only, or with an additional mild immunosuppression of 350 rads irradiation. Control groups received rat immunoglobulin or 350 rads irradiation. Graft survival and the phenotype of infiltrating cells were assessed histologically and immunocytochemically on days 12, 17, and 21, and soluble IL-2R levels were measured in the serum with a quantitative ELISA in all recipients. Two of five grafts in the AMT-13-treated group had islets on day 12 posttransplantation despite lymphocytic infiltration in all grafts, while at this time all grafts of rat Ig treated control mice were completely rejected with only scar tissue and a few lymphocytes remaining. Additional immunosuppression with 350 rads irradiation had a marked additive effect with AMT-13. Soluble IL-2R levels in the serum of untreated recipients were not elevated compared with normal serum levels, but recipients injected with AMT-13 had multifold increased soluble IL-2R levels. The percentage of IL-2R+ cells in the grafts of AMT-13-treated animals was either normal (less than 5%) or increased (20%) in the additionally irradiated mice, providing strong evidence that the immunosuppressive effect of AMT-13 is not due to a depletion of activated IL-2R+ lymphocytes.

  16. Comparison of 20 nm silver nanoparticles synthesized with and without a gold core. Structure, dissolution in cell culture media, and biological impact on macrophages

    SciTech Connect (OSTI)

    Munusamy, Prabhakaran; Wang, Chongmin; Engelhard, Mark H.; Baer, Donald R.; Smith, Jordan N.; Liu, Chongxuan; Kodali, Vamsi K.; Thrall, Brian D.; Chen, Shu; Porter, Alexandra E.; Ryan, Mary P.

    2015-07-15

    Widespread use of silver nanoparticles raises questions of environmental impact and toxicity. Both silver particles and silver ions formed by particle dissolution may impact biological systems. Therefore it is important to understand the characteristics of silver nanoparticles and their stability in relevant media. The synthesis route can impact physical and chemical characteristics of the particles and we report the characterization and solution stability of three types of silver nanoparticles (20 nm particles with and without gold cores and 110 nm particles with gold cores) in cell culture media with serum proteins: FBS10%/RPMI. These nanoparticles were synthesized in aqueous solution and characterized using both in situ and ex situ analysis methods. Dissolution studies were carried at particle concentrations from 1 µg/ml to 50 µg/ml. Particles with gold cores had smaller crystallite size and higher apparent solubility than pure silver particles. A dissolution model was found to describe the time variation of particle size and amount of dissolved silver for particle loadings above 9 µg/ml. An effective solubility product obtained from fitting the data was higher for the 20 nm gold core particles in comparison to the pure silver or 110 nm particles. Dissolution of the nanoparticles was enhanced by presence of serum proteins contained in fetal bovine serum. In addition, the protocol of the dispersion in the medium was found to influence particle agglomeration and dissolution. Results show that particle structure can impact the concentration of dissolved silver and the dose to which cells would be exposed during in vitro studies.

  17. Comparison of 20 nm silver nanoparticles synthesized with and without a gold core. Structure, dissolution in cell culture media, and biological impact on macrophages

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Munusamy, Prabhakaran; Wang, Chongmin; Engelhard, Mark H.; Baer, Donald R.; Smith, Jordan N.; Liu, Chongxuan; Kodali, Vamsi K.; Thrall, Brian D.; Chen, Shu; Porter, Alexandra E.; et al

    2015-07-15

    Widespread use of silver nanoparticles raises questions of environmental impact and toxicity. Both silver particles and silver ions formed by particle dissolution may impact biological systems. Therefore it is important to understand the characteristics of silver nanoparticles and their stability in relevant media. The synthesis route can impact physical and chemical characteristics of the particles and we report the characterization and solution stability of three types of silver nanoparticles (20 nm particles with and without gold cores and 110 nm particles with gold cores) in cell culture media with serum proteins: FBS10%/RPMI. These nanoparticles were synthesized in aqueous solution andmore » characterized using both in situ and ex situ analysis methods. Dissolution studies were carried at particle concentrations from 1 µg/ml to 50 µg/ml. Particles with gold cores had smaller crystallite size and higher apparent solubility than pure silver particles. A dissolution model was found to describe the time variation of particle size and amount of dissolved silver for particle loadings above 9 µg/ml. An effective solubility product obtained from fitting the data was higher for the 20 nm gold core particles in comparison to the pure silver or 110 nm particles. Dissolution of the nanoparticles was enhanced by presence of serum proteins contained in fetal bovine serum. In addition, the protocol of the dispersion in the medium was found to influence particle agglomeration and dissolution. Results show that particle structure can impact the concentration of dissolved silver and the dose to which cells would be exposed during in vitro studies.« less

  18. Improving in vitro Sertoli cell/gonocyte co-culture model for assessing male reproductive toxicity: Lessons learned from comparisons of cytotoxicity versus genomic responses to phthalates

    SciTech Connect (OSTI)

    Yu Xiaozhong; Hong, Sung Woo; Moreira, Estefania G.; Faustman, Elaine M.

    2009-09-15

    Gonocytes exist in the neonatal testis and represent a transient population of male germ-line stem cells. It has been shown that stem cell self-renewal and progeny production is probably controlled by the neighboring differentiated cells and extracellular matrix (ECM) in vivo known as niches. Recently, we developed an in vitro three-dimensional (3D) Sertoli cell/gonocyte co-culture (SGC) model with ECM overlay, which creates an in vivo-like niche and supports germ-line stem cell functioning within a 3D environment. In this study, we applied morphological and cytotoxicity evaluations, as well as microarray-based gene expression to examine the effects of different phthalate esters (PE) on this model. Known in vivo male developmentally toxic PEs (DTPE) and developmentally non-toxic PEs (DNTPE) were evaluated. We observed that DTPE induced significantly greater dose-dependent morphological changes, a decrease in cell viability and an increase in cytotoxicity compared to those treated with DNTPE. Moreover, the gene expression was more greatly altered by DTPE than by DNTPE and non-supervised cluster analysis allowed the discrimination of DTPE from the DNTPE. Our systems-based GO-Quant analysis showed significant alterations in the gene pathways involved in cell cycle, phosphate transport and apoptosis regulation with DTPE but not with DNTPE treatment. Disruptions of steroidogenesis related-gene expression such as Star, Cyp19a1, Hsd17b8, and Nr4a3 were observed in the DTPE group, but not in the DNTPE group. In summary, our observation on cell viability, cytotoxicity, and microarray-based gene expression analysis induced by PEs demonstrate that our in vitro 3D-SGC system mimicked in vivo responses for PEs and suggests that the 3D-SGC system might be useful in identifying developmental reproductive toxicants.

  19. Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002

    SciTech Connect (OSTI)

    Therien, Jesse B.; Zadvornyy, Oleg A.; Posewitz, Matthew C.; Bryant, Donald A.; Peters, John W.

    2014-10-18

    The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. We demonstrate the growth of C. reinhardtii on acetate provided by mutant strains of the cyanobacterium Synechococcus sp. PCC7002.

  20. Bisphenol A down-regulates rate-limiting Cyp11a1 to acutely inhibit steroidogenesis in cultured mouse antral follicles

    SciTech Connect (OSTI)

    Peretz, Jackye; Flaws, Jodi A.

    2013-09-01

    Bisphenol A (BPA) is the backbone of polycarbonate plastic products and the epoxy resin lining of aluminum cans. Previous studies have shown that exposure to BPA decreases sex steroid hormone production in mouse antral follicles. The current study tests the hypothesis that BPA first decreases the expression levels of the steroidogenic enzyme cytochrome P450 side-chain cleavage (Cyp11a1) and steroidogenic acute regulatory protein (StAR) in mouse antral follicles, leading to a decrease in sex steroid hormone production in vitro. Further, the current study tests the hypothesis that these effects are acute and reversible after removal of BPA. Exposure to BPA (10 μg/mL and 100 μg/mL) significantly decreased expression of Cyp11a1 and StAR beginning at 18 h and 72 h, respectively, compared to controls. Exposure to BPA (10 μg/mL and 100 μg/mL) significantly decreased progesterone levels beginning at 24 h and decreased androstenedione, testosterone, and estradiol levels at 72 h and 96 h compared to controls. Further, after removing BPA from the culture media at 20 h, expression of Cyp11a1 and progesterone levels were restored to control levels by 48 h and 72 h, respectively. Additionally, expression of StAR and levels of androstenedione, testosterone, and estradiol never decreased compared to controls. These data suggest that BPA acutely decreases expression of Cyp11a1 as early as 18 h and this reduction in Cyp11a1 may lead to a decrease in progesterone production by 24 h, followed by a decrease in androstenedione, testosterone, and estradiol production and expression of StAR at 72 h. Therefore, BPA exposure likely targets Cyp11a1 and steroidogenesis, but these effects are reversible with removal of BPA exposure. - Highlights: • BPA may target Cyp11a1 to inhibit steroidogenesis in antral follicles. • BPA may decrease the expression of Cyp11a1 prior to inhibiting steroidogenesis. • The adverse effects of BPA on steroidogenesis in antral follicles are reversible.

  1. Application of the Accurate Mass and Time Tag Approach to the Proteome Analysis of Sub-cellular Fractions Obtained from Rhodobacter sphaeroides 2.4.1 Aerobic and Photosynthetic Cell Cultures

    SciTech Connect (OSTI)

    Callister, Stephen J.; Dominguez, Migual; Nicora, Carrie D.; Zeng, Xiaohua; Tavano, Christine; Kaplan, Samuel; Donohue, Timothy; Smith, Richard D.; Lipton, Mary S.

    2006-08-04

    Abstract The high-throughput accurate mass and time tag (AMT) proteomic approach was utilized to characterize the proteomes for cytoplasm, cytoplasmic membrane, periplasm, and outer membrane fractions from aerobic and photosynthetic cultures of the gram-nagtive bacterium Rhodobacter sphaeroides 2.4.1. In addition, we analyzed the proteins within purified chromatophore fractions that house the photosynthetic apparatus from photosynthetically grown cells. In total, 8300 peptides were identified with high confidence from at least one sub-cellular fraction from either cell culture. These peptides were derived from 1514 genes or 35% percent of proteins predicted to be encoded by the genome. A significant number of these proteins were detected within a single sub-cellular fraction and their localization was compared to in-silico predictions. However, the majority of proteins were observed in multiple sub-cellular fractions, and the most likely sub-cellular localization for these proteins was investigated using a Z-score analysis of peptide abundance along with clustering techniques. Good (81%) agreement was observed between the experimental results and in-silico predictions. The AMT tag approach provides localization evidence for those proteins that have no predicted localization information, those annotated as putative proteins, and/or for those proteins annotated as hypothetical and conserved hypothetical.

  2. ESV Group | Open Energy Information

    Open Energy Info (EERE)

    ESV Group Jump to: navigation, search Name: ESV Group Place: London, England, United Kingdom Zip: W1K 4QH Sector: Biofuels Product: UK-based investment agri-business involved in...

  3. CX-001910: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Lincolnland Agri-Energy, LLCCX(s) Applied: B5.1Date: 03/12/2010Location(s): Palestine, IllinoisOffice(s): Energy Efficiency and Renewable Energy, Golden Field Office

  4. EIS-0300: Withdrawal of Notice of Intent to Prepare an Environmental...

    Broader source: Energy.gov (indexed) [DOE]

    Proposed Minnesota Agri-Power Plant and Associated Facilities EIS-0300-WdNOI-1999.pdf (20.67 KB) More Documents & Publications EIS-0238: Withdrawal of Notice of Intent to Prepare ...

  5. GS Global Biodiesel JV | Open Energy Information

    Open Energy Info (EERE)

    Global Biodiesel JV Jump to: navigation, search Name: GS Global Biodiesel JV Place: Iowa Product: JV between GS AgriFuels and Global Ethanol set-up to develop a plant that will...

  6. Rose Energy | Open Energy Information

    Open Energy Info (EERE)

    Kingdom Sector: Biomass Product: Backed by a consortium of three players in our agri-food industry, Rose Energy has proposed a 30MW biomass plant in Northern Ireland....

  7. Kentucky's 1st congressional district: Energy Resources | Open...

    Open Energy Info (EERE)

    in Kentucky's 1st congressional district Commonwealth AgriEnergy Four Rivers BioEnergy Retrieved from "http:en.openei.orgwindex.php?titleKentucky%27s1stcongressiona...

  8. CX-003880: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Energy Independent Agri-Business OutreachCX(s) Applied: A9, B5.1Date: 09/01/2010Location(s): Cortland County, New YorkOffice(s): Energy Efficiency and Renewable Energy, Golden Field Office

  9. FORMERLY UTILIZED SITES REMEDIAL ACTION PROGRAM ELIMINATION REPORT

    Office of Legacy Management (LM)

    - ELIMINATION REPORT FOR USS AGRI-CHEMICALS (THE FORMER ARMOUR FERTILIZER ' WORKS) BARTOW, FLORIDA NOv 26 1985 Department of Energy Office of Nuclear Energy Office of Remedial Action and Waste Technology Division of Facility and Site Decommissioning Projects -.. ." __.-__.._ ..- --- CONTENTS INTRODUCTION BACKGROUND Site Function Site Description Radiological History and Status ELIMINATION ANALYSIS REFERENCES ii Page 4 _ 5 _-_".-. .-.. ELIMINATION REPORT USS AGRI-CHEMICALS (THE FORMER

  10. Papua New Guinea-Pilot Program for Climate Resilience (PPCR)...

    Open Energy Info (EERE)

    Development (IISD) Sector Climate, Energy, Land, Water Focus Area Renewable Energy, Agriculture, Biomass, - Waste to Energy, Economic Development, Forestry, Greenhouse...

  11. LBNL International Energy Studies | Open Energy Information

    Open Energy Info (EERE)

    Lawrence Berkeley National Laboratory Sector Energy, Land Focus Area Energy Efficiency, Forestry Topics Implementation, GHG inventory, Policiesdeployment programs,...

  12. BETO Project Management Review

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... livestock operations * Organic wastes from industrial operations, including but ... biomass potential supply to 2040 * Agricultural, forestry, algal, and waste resources ...

  13. Rethinking Forest Partnerships and Benefit Sharing | Open Energy...

    Open Energy Info (EERE)

    Land Focus Area: Forestry Topics: Implementation, Resource assessment Resource Type: Lessons learnedbest practices Website: www.profor.infoproforsitesprofor.infofiles...

  14. How Communities Manage Forests | Open Energy Information

    Open Energy Info (EERE)

    FORZA Sector: Land Focus Area: Forestry Topics: Background analysis Resource Type: Lessons learnedbest practices Website: www.rightsandresources.orgdocumentsfiles...

  15. Brazil LULUCF Modeling | Open Energy Information

    Open Energy Info (EERE)

    Forestry, Transportation Topics: Background analysis, Market analysis Resource Type: Lessons learnedbest practices Website: www.esmap.orgesmapsitesesmap.orgfiles...

  16. Poverty and Forests Linkages | Open Energy Information

    Open Energy Info (EERE)

    Forestry Topics: Co-benefits assessment, Background analysis Resource Type: Publications, Lessons learnedbest practices Website: www.profor.infoproforDocumentspdflivelihoods...

  17. Building REDD Capacity in Developing Countries | Open Energy...

    Open Energy Info (EERE)

    Land Focus Area: Forestry Topics: Policiesdeployment programs Resource Type: Workshop, Lessons learnedbest practices Website: www.iisd.orgclimatelanduseredd Country:...

  18. Governance of Forests Initiative | Open Energy Information

    Open Energy Info (EERE)

    Focus Area Forestry Topics Implementation, Policiesdeployment programs Resource Type Lessons learnedbest practices Website http:www.wri.orgprojectgov Country Brazil,...

  19. A Nested Approach to REDD+: Structuring Effective and Transparent...

    Open Energy Info (EERE)

    Focus Area: Forestry Topics: Implementation, Policiesdeployment programs Resource Type: Lessons learnedbest practices Website: www.forestcarbonportal.comresource...

  20. Salish & Kootenai Holding Company - Biomass Feasibility Analysis...

    Office of Environmental Management (EM)

    Project Overview Resources Demand Analysis Technology Characterization Regulatory Permitting Economics Business Case 18 Potential Thermal Loads Tribal forestry greenhouse Public ...

  1. Cultural Resources Review for Closure of the nonradioactive Dangerous Waste Landfill and Solid Waste Landfill in the 600 Area, Hanford Site, Benton County, Washington, HCRC# 2010-600-018R

    SciTech Connect (OSTI)

    Gutzeit, Jennifer L.; Kennedy, Ellen P.; Bjornstad, Bruce N.; Sackschewsky, Michael R.; Sharpe, James J.; DeMaris, Ranae; Venno, M.; Christensen, James R.

    2011-02-02

    The U.S. Department of Energy Richland Operations Office is proposing to close the Nonradioactive Dangerous Waste Landfill (NRDWL) and Solid Waste Landfill (SWL) located in the 600 Area of the Hanford Site. The closure of the NRDWL/SWL entails the construction of an evapotranspiration cover over the landfill. This cover would consist of a 3-foot (1-meter) engineered layer of fine-grained soil, modified with 15 percent by weight pea gravel to form an erosion-resistant topsoil that will sustain native vegetation. The area targeted for silt-loam borrow soil sits in Area C, located in the northern central portion of the Fitzner/Eberhardt Arid Lands Ecology (ALE) Reserve Unit. The pea gravel used for the mixture will be obtained from both off-site commercial sources and an active gravel pit (Pit #6) located just west of the 300 Area of the Hanford Site. Materials for the cover will be transported along Army Loop Road, which runs from Beloit Avenue (near the Rattlesnake Barricade) east-northeast to the NRDWL/SWL, ending at State Route 4. Upgrades to Army Loop Road are necessary to facilitate safe bidirectional hauling traffic. This report documents a cultural resources review of the proposed activity, conducted according to Section 106 of the National Historic Preservation Act of 1966.

  2. External Assessment of Nuclear Safety Culture

    Broader source: Energy.gov [DOE]

    Presenter: Ward Sproat, Principal Vice President and Deputy Project Director for Design and Operations Waste Treatment Plant Project Bechtel National, Inc.

  3. Culturing photosynthetic bacteria through surface plasmon resonance

    SciTech Connect (OSTI)

    Ooms, Matthew D.; Bajin, Lauren; Sinton, David

    2012-12-17

    In this work, cultivation of photosynthetic microbes in surface plasmon enhanced evanescent fields is demonstrated. Proliferation of Synechococcus elongatus was obtained on gold surfaces excited with surface plasmons. Excitation over three days resulted in 10 {mu}m thick biofilms with maximum cell volume density of 20% vol/vol (2% more total accumulation than control experiments with direct light). Collectively, these results indicate the ability to (1) excite surface-bound cells using plasmonic light fields, and (2) subsequently grow thick biofilms by coupling light from the surface. Plasmonic light delivery presents opportunities for high-density optofluidic photobioreactors for microalgal analysis and solar fuel production.

  4. OPSEC and Culture in Control Systems

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    or implied, or assumes any legal liability or responsibility ... 21 3.3 Training and Awareness ... is brought back online in a consistent and stable state. ...

  5. Welcome to the Cultural Resources Newsletter

    Broader source: Energy.gov (indexed) [DOE]

    ... From l to r in the photo above are Weisskopf, Mitchell, Hartman, and Gosling. Other DOE-related sessions included presentations by Art Wolf, Director of the soon-to-be-dedicated ...

  6. Tips on Dealing with Culture Shock

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Energy Tips for Running an Air Conditioner Without Breaking the Bank Tips for Running an Air Conditioner Without Breaking the Bank July 22, 2014 - 3:15pm Addthis Cooling your home doesn't have to break the bank, with these tips you can save money and stay comfortable.| Photo courtesy of ©iStockphoto.com/galinast Cooling your home doesn't have to break the bank, with these tips you can save money and stay comfortable.| Photo courtesy of ©iStockphoto.com/galinast Elizabeth Spencer

  7. Making Energy Efficiency Part of Corporate Culture

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Nissan Green Program 7 Nissan Green Program - CO2 Reduction 8 Cultivating the Corporate ... Team leaders represent primary consumers 11 Cross-Functional Energy Team ...

  8. Fermilab Cultural Events in Batavia, IL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Eugene Cheng, Scientist in Residence, School of the Art Institute, Chicago and Professor, University of Sheffield, UK - July 15, 2016 @ 8 pm - 7 Lecture by Vanessa Hill of Brain ...

  9. Bogor Barat, Indonesia: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    a city in Indonesia. Registered Policy Organizations in Bogor Barat, Indonesia Center for International Forestry Research References "NGA Geonames Search" Retrieved from...

  10. Word Pro - Untitled1

    U.S. Energy Information Administration (EIA) Indexed Site

    of carbon that is not fully recaptured in subsequent use of the land for agriculture. ... category "agriculture, forestry, and other land use," usually based on estimates of net ...

  11. USDA and DOE Biomass Research And Development Technical Advisory...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Science, Technology and Environmental Policy Program, Woodrow Wilson School of Public ... University of New York College of Environmental Science and Forestry, Syracuse, N.Y. Read ...

  12. 2014 Race to Zero Student Design Competition: Montage Builders...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Montage Builders - Northern Forest, SUNY College of Environmental Science and Forestry, Syracuse University, Onondaga Community College. rtzmontagesummary.pdf (910.1 KB) More ...

  13. Measurement and Monitoring of the World's Forests: A Review and...

    Open Energy Info (EERE)

    Technical Capability, 2009-2015 AgencyCompany Organization: Resources for the Future Sector: Land Focus Area: Forestry Topics: Technology characterizations Resource Type:...

  14. Hae In Corp Haein Corp | Open Energy Information

    Open Energy Info (EERE)

    137-130 Product: Korean manufacturer of heavy equipments for the construction, mining, civil engineering, agriculture, fishery, forestry, livestock and power generation sectors....

  15. Forest Cover and Deforestation in Belize: 1980-2010 | Open Energy...

    Open Energy Info (EERE)

    of Latin America & the Caribbean (CATHALAC), National Aeronautics and Space Administration Sector Land Focus Area Forestry Topics Co-benefits assessment, GHG inventory,...

  16. Browse by Discipline -- E-print Network Subject Pathways: Power...

    Office of Scientific and Technical Information (OSTI)

    ... Columbia Pereira, Henrique Miguel (Henrique Miguel Pereira) - Centro de Biologia Ambiental, Universidade de Lisboa Perera, Priyan (Priyan Perera) - Department of Forestry and ...

  17. Carbon Capital | Open Energy Information

    Open Energy Info (EERE)

    Capital Jump to: navigation, search Name: Carbon Capital Place: United Kingdom Sector: Carbon Product: Manages a carbon fund specialised in forestry projects References: Carbon...

  18. Bibliography, Bioenergy Technologies Office Multi-Year Program...

    Broader source: Energy.gov (indexed) [DOE]

    ... Lindstr.m , E.; Larsson, S.; Bostrom, D.; Ohman, M. (2010). "Slagging Characteristics During Combustion of Woody Biomass Pellets Made from a Range of Different Forestry ...

  19. Bioenergy Technologies Office Multi-Year Program Plan, March...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... to an expanding market for American and Canadian wood pellets and raw biomass feedstock. ... During Combustion of Woody Biomass Pellets Made from a Range of Different Forestry ...

  20. Bioenergy Technologies Office Multi-Year Program Plan: July 2014...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... to an expanding market for American and Canadian wood pellets and raw biomass feedstock. ... Combustion of Woody Biomass Pellets Made from a Range of Different Forestry Assortments. ...

  1. Microsoft PowerPoint - Biomass Resource Assessments and What...

    Office of Environmental Management (EM)

    contracts * Handshake, purchase order * One-year contract * Prices * FOB 135-150ton Pellets * Sources * Forestry residual * Commercial logging * Fuels reduction * Restoration * ...

  2. Solutions from the Land: Developing a New Vision for United States...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Solutions from the Land: Developing a New Vision for United States Agriculture, Forestry, and Conservation Solutions from the Land: Developing a New Vision for United States ...

  3. ArborGen | Open Energy Information

    Open Energy Info (EERE)

    in research, development and commercialization of applications and solutions in tree genetics, including varietal forestry, that improve wood growth and quality for the forest...

  4. Developing Effective Forest Policy-A Guide | Open Energy Information

    Open Energy Info (EERE)

    Developing Effective Forest Policy-A Guide AgencyCompany Organization: Food and Agriculture Organization of the United Nations Sector: Land Focus Area: Forestry Topics:...

  5. TERRASTAT | Open Energy Information

    Open Energy Info (EERE)

    Tool Summary LAUNCH TOOL Name: TERRASTAT AgencyCompany Organization: Food and Agriculture Organization of the United Nations Sector: Land Focus Area: Forestry, Agriculture...

  6. Uganda-USAID Climate Activities | Open Energy Information

    Open Energy Info (EERE)

    U.S. Agency for International Development Sector Land Focus Area Forestry, Agriculture Topics Background analysis Website http:www.usaid.govourwork Country Uganda...

  7. AquaStat | Open Energy Information

    Open Energy Info (EERE)

    Tool Summary LAUNCH TOOL Name: AquaStat AgencyCompany Organization: Food and Agriculture Organization of the United Nations Sector: Land Focus Area: Forestry, Agriculture...

  8. Guinea-USAID Climate Activities | Open Energy Information

    Open Energy Info (EERE)

    International Development Sector Energy, Land Focus Area Renewable Energy, Forestry, Agriculture Topics Background analysis Website http:www.usaid.govourwork Country Guinea...

  9. Grupo Plantar | Open Energy Information

    Open Energy Info (EERE)

    Plantar Jump to: navigation, search Name: Grupo Plantar Place: Gutierrez, Minas Gerais, Brazil Product: Brazil-based forestry developer that runs a pig iron production plant on...

  10. Biomass: Wood as Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Coordinator USDA Forest Service State & Private Forestry ... habitat and forest health Modern Woody Biomass ... Requires manual fuel delivery & stoking Pellets Meter ...

  11. Ecotrust | Open Energy Information

    Open Energy Info (EERE)

    Name: Ecotrust Place: Portland, Oregon Zip: 97209 Product: Leading conversation organization with focus on forestry, fisheries and other projects primarily in the Pacific...

  12. Bioenergy Sustainability Analysis | Bioenergy | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... The conservation of agricultural soils and afforestation have the potential to increase ... are part of agriculture, forestry, and management of rural and urban residues and wastes. ...

  13. REDD Glossary | Open Energy Information

    Open Energy Info (EERE)

    AgencyCompany Organization: Pact Sector: Land Focus Area: Forestry Resource Type: Guidemanual, Training materials Website: pactworld.orgcsreddglossary REDD Glossary...

  14. Colorado Renewable Resource Cooperative | Open Energy Information

    Open Energy Info (EERE)

    Product: Colorado-based cooperative and forestry producer, that targets the use of woody biomass to generate heat or electricity. References: Colorado Renewable Resource...

  15. Property:OpenEI/Tool/RelatedTo | Open Energy Information

    Open Energy Info (EERE)

    Forestry and Other Land Use Mitigation Project Database + Mitigation of Climate Change in Agriculture (MICCA) Project + Applied Dynamic Analysis of the Global Economy...

  16. The REDD Opportunities Scoping Exercise (ROSE) | Open Energy...

    Open Energy Info (EERE)

    Tanzania, and Uganda AgencyCompany Organization The Katoomba Group, Forest Trends, Nature Conservation Research Centre Sector Land Focus Area Forestry Topics Implementation,...

  17. Program on Forests | Open Energy Information

    Open Energy Info (EERE)

    the Japanese International Forestry Cooperation Office, the Ministry of Agriculture, Nature and Food Quality of the Netherlands, and the Swiss Development Cooperation (SDC). The...

  18. Costa Rica-Mitigation of Greenhouse Gas Emissions through Avoided...

    Open Energy Info (EERE)

    Value Areas AgencyCompany Organization Government of Costa Rica, Peace with Nature Sector Land Focus Area Forestry Topics Co-benefits assessment, Implementation,...

  19. REDD+ Training Materials | Open Energy Information

    Open Energy Info (EERE)

    for International Cooperation (GIZ), World Wildlife Fund, The Rainforest Alliance, The Nature Conservancy, Conservation International Sector: Land Focus Area: Forestry Resource...

  20. Legal Frameworks for REDD: Design and Implementation at the National...

    Open Energy Info (EERE)

    National Level AgencyCompany Organization: International Union for Conservation of Nature Sector: Land Focus Area: Forestry Topics: Implementation Resource Type: Publications...

  1. Democratic Republic of Congo-Forest Investment Program (FIP)...

    Open Energy Info (EERE)

    World Bank, African Development Bank (AfDB) Partner Ministry of Environment, Nature Conservation and Tourism Sector Land Focus Area Biomass, Forestry, Greenhouse Gas,...

  2. UNDP/GEF-Cambodia-Sustainable Forest Management | Open Energy...

    Open Energy Info (EERE)

    be carried out through community forestry activities to engage rural communities in nature conservation and create markets for sustainable bio-energy technologies to help curb...

  3. SREL Reprint #3188

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA 2Department of Environmental Health Science, University of Georgia, Athens, GA 30602,...

  4. SREL Reprint #3172

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA 2Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802,...

  5. International Forest Policy Database | Open Energy Information

    Open Energy Info (EERE)

    Database Jump to: navigation, search Tool Summary LAUNCH TOOL Name: International Forest Policy Database AgencyCompany Organization: GTZ Sector: Land Focus Area: Forestry...

  6. Brazil-Low Carbon Growth Studies Program | Open Energy Information

    Open Energy Info (EERE)

    and forestry (LULUCF), including deforestation; (ii) transport systems; (iii) energy production and use, particularly electricity, oil and gas and bio-fuels; and (iv) solid...

  7. Environmental Programs

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Los Alamos County Fire Department provides wildfire suppression support. The New Mexico Joint Powers Agreement, between the state Forestry Division, DOE, the Department of...

  8. General Equilibrium Emissions Model (GEEM) | Open Energy Information

    Open Energy Info (EERE)

    Development (IISD) Sector: Climate, Energy Focus Area: Renewable Energy, Non-renewable Energy, Agriculture, Buildings, Economic Development, Energy Efficiency, Forestry, Goods...

  9. Bangladesh-NAMA Concepts | Open Energy Information

    Open Energy Info (EERE)

    for Sustainable Development (IISD) Sector Climate, Energy, Land Focus Area Non-renewable Energy, Agriculture, Buildings, Energy Efficiency, Forestry, Greenhouse Gas, Industry,...

  10. Pathways for Implementing REDD+: Experiences from Carbon Markets...

    Open Energy Info (EERE)

    Centre Sector: Land Focus Area: Forestry Topics: Finance, Implementation Resource Type: Lessons learnedbest practices Website: www.acp-cd4cdm.orgmedia237951...

  11. IPCC Inventory Guidelines LULUCF | Open Energy Information

    Open Energy Info (EERE)

    Land Focus Area: Forestry, Agriculture Topics: GHG inventory Resource Type: Guidemanual, Lessons learnedbest practices Website: www.ipcc-nggip.iges.or.jppublicgpglulucf...

  12. Forest Tenure Reform in Vietnam | Open Energy Information

    Open Energy Info (EERE)

    Land Focus Area Forestry Topics Resource assessment, Background analysis Resource Type Lessons learnedbest practices Website http:recoftc.orgsitefilead Country Vietnam UN...

  13. Forest Carbon Portal | Open Energy Information

    Open Energy Info (EERE)

    Forest Trends Sector: Land Focus Area: Forestry Topics: GHG inventory Resource Type: Lessons learnedbest practices Website: www.forestcarbonportal.com Forest Carbon Portal...

  14. REDD Collaborative Online Workspace | Open Energy Information

    Open Energy Info (EERE)

    Nations Environment Programme Sector: Land Focus Area: Biomass, Forestry Resource Type: Lessons learnedbest practices Website: www.unredd.netindex.php?optioncomdocman&taskca...

  15. Green Economy: Developing Country Success Stories | Open Energy...

    Open Energy Info (EERE)

    Solar, Buildings, Forestry, Agriculture Topics: Background analysis Resource Type: Lessons learnedbest practices Website: www.unep.orgpdfGreenEconomySuccessStories.pdf...

  16. Action Plan for Forest Law Enforcement Governance and Trade ...

    Open Energy Info (EERE)

    Union Sector Land Focus Area Forestry Topics Policiesdeployment programs Resource Type Lessons learnedbest practices Website http:eur-lex.europa.eusmart Country Belgium,...

  17. Cameroon-Forest Sector Development in a Difficult Political Economy...

    Open Energy Info (EERE)

    Bank Sector Land Focus Area Forestry Topics Implementation, Market analysis Resource Type Lessons learnedbest practices Website http:lnweb90.worldbank.orgo Country Cameroon UN...

  18. Sustainable Nanomaterials Industry Perspective

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Industry Perspective U.S. Department of Energy Advanced Manufacturing Office Sustainable ... Uses renewable resources grown with sustainable forestry practices Encourages ...

  19. Emily Therese Cloyd | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Emily holds a Master's degree in Conservation Biology (State University of New York College of Environmental Science and Forestry) and a Bachelor's degree in Plant Biology ...

  20. Maisah Khan | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    the Yale School of Forestry and Environmental Studies. She graduated summa cum laude from Arizona State University with a B.S. in Conservation Biology and Ecological Sustainability

  1. Brazil Timber | Open Energy Information

    Open Energy Info (EERE)

    Timber Jump to: navigation, search Name: Brazil Timber Place: Sao Paulo, Brazil Zip: 04562-030 Product: Brazil-based forestry industry consultancy. References: Brazil Timber1...

  2. Hdom | Open Energy Information

    Open Energy Info (EERE)

    Hdom Jump to: navigation, search Name: Hdom Place: Manaus, Amazonas, Brazil Product: Brazil based forestry consultancy company. References: Hdom1 This article is a stub. You can...

  3. Hybrid Poplar Research

    SciTech Connect (OSTI)

    2006-09-01

    This Congressionally-mandated project focuses on characterizing and improving hybrid poplar plantation forestry systems with the ultimate goal of using poplars as a dedicated energy crop.

  4. Enviva Materials LLC | Open Energy Information

    Open Energy Info (EERE)

    LLC Place: Richmond, Virginia Zip: 23219 Sector: Biomass Product: Recovering of agricultural, forestry and industrial byproducts in order to supply the biomass power industry....

  5. Singapore-Low Carbon Asia Research Network (LoCARNet) | Open...

    Open Energy Info (EERE)

    Energy, Land Focus Area Agriculture, Biomass, Buildings, Economic Development, Energy Efficiency, Forestry, Geothermal, Greenhouse Gas, Hydrogen, Industry, Land Use, People and...

  6. Myanmar-Low Carbon Asia Research Network (LoCARNet) | Open Energy...

    Open Energy Info (EERE)

    Energy, Land Focus Area Agriculture, Biomass, Buildings, Economic Development, Energy Efficiency, Forestry, Geothermal, Greenhouse Gas, Hydrogen, Industry, Land Use, People and...

  7. Cambodia-Low Carbon Asia Research Network (LoCARNet) | Open Energy...

    Open Energy Info (EERE)

    Energy, Land Focus Area Agriculture, Biomass, Buildings, Economic Development, Energy Efficiency, Forestry, Geothermal, Greenhouse Gas, Hydrogen, Industry, Land Use, People and...

  8. Brunei-Low Carbon Asia Research Network (LoCARNet) | Open Energy...

    Open Energy Info (EERE)

    Energy, Land Focus Area Agriculture, Biomass, Buildings, Economic Development, Energy Efficiency, Forestry, Geothermal, Greenhouse Gas, Hydrogen, Industry, Land Use, People and...

  9. ClimateTechWiki | Open Energy Information

    Open Energy Info (EERE)

    Climate, Energy Focus Area: Non-renewable Energy, Agriculture, Biomass, Buildings, Energy Efficiency, Forestry, Geothermal, Greenhouse Gas, Ground Source Heat Pumps, Hydrogen,...

  10. Vietnam-Low Carbon Asia Research Network (LoCARNet) | Open Energy...

    Open Energy Info (EERE)

    Energy, Land Focus Area Agriculture, Biomass, Buildings, Economic Development, Energy Efficiency, Forestry, Geothermal, Greenhouse Gas, Hydrogen, Industry, Land Use, People and...

  11. Malaysia-Low Carbon Asia Research Network (LoCARNet) | Open Energy...

    Open Energy Info (EERE)

    Energy, Land Focus Area Agriculture, Biomass, Buildings, Economic Development, Energy Efficiency, Forestry, Geothermal, Greenhouse Gas, Hydrogen, Industry, Land Use, People and...

  12. India-Low Carbon Asia Research Network (LoCARNet) | Open Energy...

    Open Energy Info (EERE)

    Energy, Land Focus Area Agriculture, Biomass, Buildings, Economic Development, Energy Efficiency, Forestry, Geothermal, Greenhouse Gas, Hydrogen, Industry, Land Use, People and...

  13. Sandbag Carbon Offset Map | Open Energy Information

    Open Energy Info (EERE)

    Organization: Sandbag Sector: Energy, Land Focus Area: Renewable Energy, Biomass, Energy Efficiency, Forestry, Geothermal, Hydrogen, Industry, Solar, Wind Topics: Market analysis...

  14. Japan-Low Carbon Asia Research Network (LoCARNet) | Open Energy...

    Open Energy Info (EERE)

    Energy, Land Focus Area Agriculture, Biomass, Buildings, Economic Development, Energy Efficiency, Forestry, Geothermal, Greenhouse Gas, Hydrogen, Industry, Land Use, People and...

  15. China-Low Carbon Asia Research Network (LoCARNet) | Open Energy...

    Open Energy Info (EERE)

    Energy, Land Focus Area Agriculture, Biomass, Buildings, Economic Development, Energy Efficiency, Forestry, Geothermal, Greenhouse Gas, Hydrogen, Industry, Land Use, People and...

  16. Laos-Low Carbon Asia Research Network (LoCARNet) | Open Energy...

    Open Energy Info (EERE)

    Energy, Land Focus Area Agriculture, Biomass, Buildings, Economic Development, Energy Efficiency, Forestry, Geothermal, Greenhouse Gas, Hydrogen, Industry, Land Use, People and...

  17. Indonesia-Low Carbon Asia Research Network (LoCARNet) | Open...

    Open Energy Info (EERE)

    Energy, Land Focus Area Agriculture, Biomass, Buildings, Economic Development, Energy Efficiency, Forestry, Geothermal, Greenhouse Gas, Hydrogen, Industry, Land Use, People and...

  18. Philippines-Low Carbon Asia Research Network (LoCARNet) | Open...

    Open Energy Info (EERE)

    Energy, Land Focus Area Agriculture, Biomass, Buildings, Economic Development, Energy Efficiency, Forestry, Geothermal, Greenhouse Gas, Hydrogen, Industry, Land Use, People and...

  19. Thailand-Low Carbon Asia Research Network (LoCARNet) | Open Energy...

    Open Energy Info (EERE)

    Energy, Land Focus Area Agriculture, Biomass, Buildings, Economic Development, Energy Efficiency, Forestry, Geothermal, Greenhouse Gas, Hydrogen, Industry, Land Use, People and...

  20. Impact of the Global Forest Industry on Atmospheric Greenhouse...

    Open Energy Info (EERE)

    or for non wood forest products may also have a considerable role in the global carbon balance, but these are beyond the scope of this publication." References "Forestry...

  1. RAPID/Roadmap/3-UT-b | Open Energy Information

    Open Energy Info (EERE)

    Toolkit BETA About Bulk Transmission Geothermal Hydropower Solar Tools Contribute Contact Us State Land Easement (3-UT-b) The Utah Division of Forestry, Fire and State Lands...

  2. Global Trade and Environmental Model (GTEM) | Open Energy Information

    Open Energy Info (EERE)

    Australia Department of Agriculture, Fisheries, and Forestry (ABARES) Sector: Climate, Energy Topics: Analysis Tools ComplexityEase of Use: Advanced Website: www.daff.gov.au...

  3. documenttemplate

    Office of Legacy Management (LM)

    ... The State is the surface operator; the Mississippi Forestry Commission is its agent. The federal government owns the monitoring wells, the monument at surface ground zero (SGZ), ...

  4. SREL Reprint #3310

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Abstract: Land at Fort Benning is used for multiple purposes. Use for military training ... These military and forestry land uses occur over a heterogeneous environment. The ...

  5. Before the Committee on Agriculture Subcommittee on General Farm...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    (69.78 KB) More Documents & Publications Before the Senate Agriculture, Nutrition, and Forestry Hearing Before the Senate Environment and Public Works Committee Before ...

  6. Low-Carbon Growth Country Studies: Getting Started Experience...

    Open Energy Info (EERE)

    as improved fiscal incentives in forestry, are also considered. Mexico's study provides a body of knowledge about prospective low carbon "wedges," specific low carbon projects, and...

  7. Feasibility for Wood Heat - Collaborative Integrated Wood Energy...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    for Wood Heat * Non-Profit Consortium of Ten Tribal ... Forestry, Fire Management, Self- Governance, ... coordination's across organizations 2 boilers and one ...

  8. Gabon-World Bank Climate Activities | Open Energy Information

    Open Energy Info (EERE)

    World Bank Climate Activities Jump to: navigation, search Name Gabon-World Bank Climate Activities AgencyCompany Organization World Bank Sector Land Focus Area Forestry Topics...

  9. Democratic Republic of Congo-ClimateWorks Low Carbon Growth Planning...

    Open Energy Info (EERE)

    Organization ClimateWorks, Project Catalyst, McKinsey and Company Sector Energy, Land Focus Area Forestry, Greenhouse Gas Topics Background analysis, Low emission...

  10. Des Moines Area Community College | OSTI, US Dept of Energy Office of

    Office of Scientific and Technical Information (OSTI)

    Scientific and Technical Information Des Moines Area Community College Spotlights Home DOE Applauds Des Moines Area Community College Science and Technical Programs Des Moines Area Community College Des Moines Area Community College Des Moines, Iowa Agri/Natural Resources Biology Biomass Operations Biotechnology Environmental Science Information Technology Manufacturing Technology Microcomputers Civil Engineering Pre-Medical Telecommunications Wind Turbines Resource Links About Library

  11. Fermilab Cultural Events in Chicago's Far West Side

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Chicago, invited her to draw and paint at the pre-history excavation in Eastern Turkey. In the 1970's she created a series of works inspired by electric circuitry and...

  12. BISfuel Collaborative Culture | Center for Bio-Inspired Solar...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Center is very collaborative. EFRC provided two things that I came to ASU for: the energy related research and the program that encourages the multidisciplinary research. It ...

  13. Northern New Mexican pueblo preserves cultural history through

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    North Carolina A&T State University Fall Career Fair North Carolina A&T State University Fall Career Fair September 14, 2016 9:00AM to 5:00PM EDT Location: 1601 E. Market Street, Greensboro, NC, 27411 Attendees: FE and EERE

    Dakota

    North Dakota and Texas help boost U.S. oil reserves to highest level since 1975 U.S. proved oil reserves have topped 36 billion barrels for the first time in nearly four decades...with North Dakota and Texas accounting for 90% of the increase

  14. Best Practices Workshop for Safety Culture | Y-12 National Security...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NPO and CNS sponsored the event, which drew participants from DOE, NNSA, the national labs (Lawrence Livermore, Oak Ridge, Sandia and Idaho), production and environmental ...

  15. Fermilab Cultural Events in Chicago's Far West Side

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2012 Artist Reception April 27, 2012 * Waterstreet Studios will present work referencing kinetic energy, the energy possessed by an object due to its motion.on display April 20,...

  16. Fermilab Cultural Events in Chicago's Far West Side

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Film Series Update The Fermilab International Film Society has come to an end. Many thanks to all who have shown their support and donated their time throughout the years....

  17. Operational Pause at Savannah River Site Benefits Safety Culture...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The K Area Complex exited deliberate operations on Jan. 25 this year. AIKEN, S.C. - EM and the Savannah River Site (SRS) management and operations contractor are seeing positive ...

  18. Fermilab Cultural Events in Chicago's Far West Side

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ticketing now available Click here to purchase tickets or call our box office at 630840.ARTS (2787). All Chamber Series concerts begin at 2:30 p.m. in the 2nd Floor Art Gallery...

  19. Northern New Mexican pueblo preserves cultural history through...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    to bring the youth academic enrichment through emphasis on science, math and language arts in addition to honoring tradition. Through the program, which offers Tewa classes in...

  20. Fermilab Cultural Events in Chicago's Far West Side

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Arts Series Presents: July 11, 2009 Ashley Lewis and Ashton Gap 16 (8 ages 18 and under) August 1, 2009 Ma Xiaohui Magic Erhu Recital 18 (9 ages 18 and under) September 19,...