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Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


1

Biodegradation of Triclosan by Aerobic Microorganisms  

E-Print Network [OSTI]

and limited. To bridge this knowledge gap, this dissertation characterized cultivable triclosan-degrading microorganisms, identified uncultivable triclosan-utilizing bacteria, and elucidated triclosan biodegradation pathways. Furthermore, two treatment...

Lee, Do Gyun

2012-10-19T23:59:59.000Z

2

Aerobic microorganism for the degradation of chlorinated aliphatic hydrocarbons  

DOE Patents [OSTI]

A chlorinated aliphatic hydrocarbon-degrading microorganism, having American Type Culture Collection accession numbers ATCC 53570 and 53571, in a biologically pure culture aseptically collected from a deep subsurface habitat and enhanced, mineralizes trichloroethylene and tetrachloroethylene to HCl, H.sub.2 O and Co.sub.2 under aerobic conditions stimulated by methane, acetate, methanol, tryptone-yeast extract, propane and propane-methane.

Fliermans, Carl B. (Augusta, GA)

1989-01-01T23:59:59.000Z

3

E-Print Network 3.0 - aerobic gram-negative bacteria Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Alcaligenes and Serratia, and a very small Gram-negative... , but aerobic, submerged cultivation of bacteria on the mesquite wood increases the digestible protein... each of...

4

Aerobic mineralization of MTBE and tert-butyl alcohol by stream-bed sediment microorganisms  

SciTech Connect (OSTI)

Microorganisms indigenous to the stream-bed sediments at two gasoline-contaminated groundwater sites demonstrated significant mineralization of the fuel oxygenates, methyl tert-butyl ether (MTBE) and tert-butyl alcohol (TBA). Up to 73% of [U-{sup 14}C]-MTBE and 84% of [U-{sup 14}C]-TBA were degraded to {sup 14}CO{sub 2} under mixed aerobic/anaerobic conditions. No significant mineralization was observed under strictly anaerobic conditions. The results indicate that, under the mixed aerobic/anaerobic conditions characteristic of stream-bed sediments, microbial processes may provide a significant environmental sink for MTBE and TBA delivered to surface water bodies by contaminated groundwater or by other sources.

Bradley, P.M.; Landmeyer, J.E.; Chapelle, F.H. [Geological Survey, Columbia, SC (United States)] [Geological Survey, Columbia, SC (United States)

1999-06-01T23:59:59.000Z

5

(Catalytic mechanism of hydrogenase from aerobic N sub 2 -fixing microorganisms)  

SciTech Connect (OSTI)

Hydrogenases are enzymes which catalyze reactions involving dihydrogen. They serve integral roles in a number of microbial metabolic pathways. Our research is focussed on investigations of the catalytic mechanism of the hydrogenases found in aerobic, N{sub 2}-fixing microorganisms such as Azotobacter vinelandii and the agronomically important Bradyrhizobium japonicum as well as microorganisms with similar hydrogenases. The hydrogenases isolated from these microorganisms are Ni- and Fe-containing heterodimers. Our work has focussed on three areas during the last grant period. In all cases, a central theme has been the role of inhibitors in the characteristics under investigation. In addition, a number of collaborative efforts have yielded interesting results. In metalloenzymes such as hydrogenase, inhibitors often influence the activity of the enzyme through ligand interactions with redox centers, often metals, within the enzyme. Therefore, investigations of the ability of various compounds to inhibit an enzyme's activity, as well as the mechanism of inhibition, can provide insight into the catalytic mechanism of the enzyme as well as the role of various redox centers in catalysis. We have investigated in detail four inhibitors of A. vinelandii and the results are summarized here. The influence of these inhibitors on the spectral properties of the enzyme are summarized. Electron paramagnetic resonance and ultraviolet spectra investigations are discussed. 9 figs.

Arp, D.J.

1990-01-01T23:59:59.000Z

6

Aerobic uranium (VI) bioprecipitation by metal-resistant bacteria isolated from radionuclide-  

E-Print Network [OSTI]

Aerobic uranium (VI) bioprecipitation by metal-resistant bacteria isolated from radionuclide uranium [U(VI)] mediated by the intrinsic phosphatase acti- vities of naturally occurring bacteria leaks occur, these wastes come into contact with surrounding geologic media, allowing for migration

Skolnick, Jeff

7

aerobic hydrogen-consuming bacteria: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

aerobic hydrogen-consuming bacteria First Page Previous Page 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 Diversity of...

8

E-Print Network 3.0 - aerobic hydrogenotroph bacteria Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

creating microenvi- ronments of aerobic conditions (Wigand et al., 1997; Jackson and Armstrong, 1999... of hydrogenotrophic methanogens (archaea that utilize H2 and CO2 as energy...

9

E-Print Network 3.0 - aerobic sporeforming bacteria Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

have spore-forming bacteria that killed... the bacteria found in the soil on bacterial agar and feeding this diverse ... Source: USDA, Forestry Service, Northern Research Station...

10

Culturing Aerobic and Anaerobic Bacteria and Mammalian Cells with a Microfluidic Differential Oxygenator  

E-Print Network [OSTI]

In this manuscript, we report on the culture of anaerobic and aerobic species within a disposable multilayer polydimethylsiloxane (PDMS) microfluidic device with an integrated differential oxygenator. A gas-filled microchannel ...

Lam, Raymond H. W.

11

Engineered microorganisms capable of producing target compounds under anaerobic conditions  

DOE Patents [OSTI]

The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

Buelter, Thomas (Denver, CO); Meinhold, Peter (Denver, CO); Feldman, Reid M. Renny (San Francisco, CA); Hawkins, Andrew C. (Parker, CO); Urano, Jun (Irvine, CA); Bastian, Sabine (Pasadena, CA); Arnold, Frances (La Canada, CA)

2012-01-17T23:59:59.000Z

12

Aerobic enhanced oil recovery: analysis of the mechanisms and a pilot study  

E-Print Network [OSTI]

The technique that uses microorganisms to improve oil production in petroleum reservoirs is known as microbial enhanced oil recovery (MEOR). Aerobic microbial enhanced oil recovery is a method which is based on stimulating indigenous oil degrading...

Eide, Karen

1998-01-01T23:59:59.000Z

13

Enhancement of Bacterial Transport in Aerobic and Anaerobic Environments: Assessing the Effect of Metal Oxide Chemical Heterogeneities  

SciTech Connect (OSTI)

The goal of our research was to understand the fundamental processes that control microbial transport in physically and chemically heterogeneous aquifers and from this enhanced understanding determine the requirements for successful, field-scale delivery of microorganisms to metal contaminated subsurface sites. Our specific research goals were to determine; (1) the circumstances under which the preferential adsorption of bacteria to Fe, Mn, and Al oxyhydroxides influences field-scale bacterial transport, (2) the extent to which the adhesion properties of bacterial cells affect field-scale bacterial transport, (3) whether microbial Fe(III) reduction can enhance field-scale transport of Fe reducing bacteria (IRB) and other microorganisms and (4) the effect of field-scale physical and chemical heterogeneity on all three processes. Some of the spin-offs from this basic research that can improve biostimulation and bioaugmentation remediation efforts at contaminated DOE sites have included; (1) new bacterial tracking tools for viable bacteria; (2) an integrated protocol which combines subsurface characterization, laboratory-scale experimentation, and scale-up techniques to accurately predict field-scale bacterial transport; and (3) innovative and inexpensive field equipment and methods that can be employed to enhance Fe(III) reduction and microbial transport and to target microbial deposition under both aerobic and anaerobic conditions.

T.C. Onstott

2005-09-30T23:59:59.000Z

14

Autoheated thermophilic aerobic digestion  

SciTech Connect (OSTI)

Autothermal thermophilic aerobic digestion (ATAD) is first and foremost a digestion process, the primary purpose of which is to decompose a portion of the waste organic solids generated from wastewater treatment. As a result of the high operating temperature, digestion is expected to occur within a short time period (6 days) and accomplish a high degree of pathogen reduction. ATAD systems are two-stage aerobic digestion processes that operate under thermophilic temperature conditions (40 to 80C) without supplemental heat. Like composting, the systems rely on the conservation of heat released during digestion itself to attain and sustain the desired operating temperature. Typical ATAD systems operate at 55C and may reach temperatures of 60 to 65C in the second-stage reactor. Perhaps because of the high operating temperature, this process has been referred to as Liquid Composting.' Major advantages associated with thermophilic operation include high biological reaction rates and a substantial degree of pathogen reduction.

Deeny, K. (Junkins Engineering, Morgantown, PA (United States)); Hahn, H.; Leonhard, D. (Univ. Karlsruhe (West Germany)); Heidman, J. (Environmental Protection Agency, Cincinnati, OH (United States))

1991-10-01T23:59:59.000Z

15

Bacteria recovered from endometritis and pyometra in the beef cow  

E-Print Network [OSTI]

One hundred and one uteri from beef cows with pyometra were collected from a slaughterhouse. Samples of uterine exudate were cultured for aerobic, microaerophilic, and anaerobic bacteria, and also tested for Trichomonas spp. A section of uterine...

Mikulec, Rashel Thi

1999-01-01T23:59:59.000Z

16

Aerobic landfill bioreactor  

DOE Patents [OSTI]

The present invention includes a method of decomposing municipal solid waste (MSW) within a landfill by converting the landfill to aerobic degradation in the following manner: (1) injecting air via the landfill leachate collection system (2) injecting air via vertical air injection wells installed within the waste mass; (3) applying leachate to the waste mass using a pressurized drip irrigation system; (4) allowing landfill gases to vent; and (5) adjusting air injection and recirculated leachate to achieve a 40% to 60% moisture level and a temperature between 120.degree. F. and 140.degree. F. in steady state.

Hudgins, Mark P (Aiken, SC); Bessette, Bernard J (Aiken, SC); March, John (Winterville, GA); McComb, Scott T. (Andersonville, SC)

2000-01-01T23:59:59.000Z

17

Aerobic landfill bioreactor  

DOE Patents [OSTI]

The present invention includes a system of decomposing municipal solid waste (MSW) within a landfill by converting the landfill to aerobic degradation in the following manner: (1) injecting air via the landfill leachate collection system (2) injecting air via vertical air injection wells installed within the waste mass; (3) applying leachate to the waste mass using a pressurized drip irrigation system; (4) allowing landfill gases to vent; and (5) adjusting air injection and recirculated leachate to achieve a 40% to 60% moisture level and a temperature between 120.degree. F. and 140.degree. F. in steady state.

Hudgins, Mark P (Aiken, SC); Bessette, Bernard J (Aiken, SC); March, John C (Winterville, GA); McComb, Scott T. (Andersonville, SC)

2002-01-01T23:59:59.000Z

18

Use of Disinfectants and Cleaners to Reduce Bacteria on Poultry Transportation Coops with a Compressed Air Foam System  

E-Print Network [OSTI]

and evaluated the treatments ability to reduce aerobic bacteria from the manure. The second study added a HPWR step to determine whether this technique would reduce bacteria. In the third study, Salmonella Typhimurium was added to the homogenized fecal slurry...

Hinojosa-Garza, Carolee A.

2013-05-24T23:59:59.000Z

19

Response of Prochlorococcus ecotypes to co-culture with diverse marine bacteria  

E-Print Network [OSTI]

Interactions between microorganisms shape microbial ecosystems. Systematic studies of mixed microbes in co-culture have revealed widespread potential for growth inhibition among marine heterotrophic bacteria, but similar ...

Sher, Daniel

20

Microorganisms for producing organic acids  

DOE Patents [OSTI]

Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.

Pfleger, Brian Frederick; Begemann, Matthew Brett

2014-09-30T23:59:59.000Z

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

Results an data on the growth of the microorganisms  

SciTech Connect (OSTI)

The study of biocorrosion of aluminum and its alloy was performed under conditions of continuous fermentation of thermophilic anaerobic microorganisms of different groups. This allowed us to examine the effect of various types of metabolic reactions of reduction-oxidation proceeding at different pH and temperatures under highly reduced conditions on aluminum corrosion. Besides, the experiments were performed where the part of the standard sample was exposed under strictly anaerobic conditions with an active microbiological process, and the second half was exposed under aerobic conditions. Thus the sample was exposed in gradient of oxidized-reduced conditions.

Laurinavichius, K.S.

1995-10-09T23:59:59.000Z

22

E-Print Network 3.0 - aerobic gammaproteobacterium congregibacter...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

behavior may be supported by ei- ther aerobic or anaerobic metabolism. Aerobic... demand and aerobic energy supply. In addition, each functional system in- volved in oxygen...

23

Expression of Genes Linked to NOx Detoxification in Aerobic Bacteria  

E-Print Network [OSTI]

operons referred to as the nif cluster. This suite of genesgenes not found in the nif clusters of K. pneumoniae (i.e.is high conservation among nif gene organization based on

Cua, Lynnie

2010-01-01T23:59:59.000Z

24

Introduction Many microorganisms including fungi and bacteria had been  

E-Print Network [OSTI]

and other plant cell wall fibers. In nature, degradation of cellulosic biomass is performed by mixtures, EGs) and exo-acting (cellobiohydrolases, CBH) enzymes, which act in a synergistic manner in biomass was targeted, (2) assays in which the reduction in substrate quantity was monitored, and (3) assays in which

Qin, Wensheng

25

E-Print Network 3.0 - anoxygenic phototrophic bacteria Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

oxygen in the atmosphere. D) anoxygenic... ) Both a and c 7. When bacteria began evolving, Earth was A) cool and anaerobic. B) warm and aerobic. C... animals. D) microbes make...

26

METHANE OXIDATION (AEROBIC) Helmut Brgmann  

E-Print Network [OSTI]

METHANE OXIDATION (AEROBIC) Helmut Bürgmann Eawag, Swiss Federal Institute of Aquatic Science and Technology, Kastanienbaum, Switzerland Synonyms Methanotrophy Definition Methane oxidation is a microbial metabolic process for energy generation and carbon assimilation from methane that is carried out by specific

Wehrli, Bernhard

27

(Catalytic mechanism of hydrogenase from aerobic N sub 2 -fixing microorganisms)  

SciTech Connect (OSTI)

The results of this DOE-sponsored project have contributed to our understanding of the catalytic mechanism of A. vinelandii hydrogenase. A group of inhibitors have been characterized. These provide information about the different types of redox clusters involved in catalysis and the roles of each. One group has already used acetylene in a study of three desulfovibrian hydrogenases and shown that only the NiFe hydrogenases are inhibited. We have characterized a number of spectral properties of A. vinelandii hydrogenase. The EPR signals associated with this hydrogenase in the reduced state are reminiscent of other NiFe dimeric hydrogenases such as A. eutrophus, but distinctly difference from others such as D. gigas and Chromatium vinosum. Thus, while the NiFe dimeric hydrogenases are now recognized as a large group of similar enzymes, there are differences in the spectral and catalytic properties which are not explained by their similar redox inventories, identical subunit structures, immunological cross reactivity and conserved sequences. The inhibitors we have characterized are also proving of value in the spectral characterizations. Surprisingly, we only see a significant EP signal attributable to Ni after the enzyme has been inactivated with O{sub 2} and then reduced (though not reactivated). No spectral perterbations (EPR or UV-V is) of active enzyme can be attributed to binding of H{sub 2}, even though H{sub 2} clearly binds to this form of the enzyme. Acetylene, which does not substantially perterb the EPR signal of active hydrogenase, does result in a new absorption envelope in the UV-V is spectrum. Overall, the results of this project have revealed the complex interactions of the redox clusters in catalysis through studies of inhibitor mechanisms and spectral properties. 14 refs., 9 figs.

Arp, D.J.

1991-01-01T23:59:59.000Z

28

Bioelectricity Aware of bacteria  

E-Print Network [OSTI]

(translation by Google Translate) Bacteria of the genus Geobacter and electrical nanowires. #12;Bioelectricity Aware of bacteria Bacteria of the genus Geobacter carry out anaerobic respiration the mechanism that makes these bacteria conductors of electricity. Researchers have studied

Lovley, Derek

29

Engineering and Coordination of Regulatory Networks and Intracellular Complexes to Maximize Hydrogen Production by Phototrophic Microorganisms  

SciTech Connect (OSTI)

This project is a collaboration with F. R. Tabita of Ohio State. Our major goal is to understand the factors and regulatory mechanisms that influence hydrogen production. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Our part of the project was to develop a modeling technique to investigate the metabolic network in connection to hydrogen production and regulation. Organisms must balance the pathways that generate and consume reducing power in order to maintain redox homeostasis to achieve growth. Maintaining this homeostasis in the nonsulfur purple photosynthetic bacteria is a complex feat with many avenues that can lead to balance, as these organisms possess versatile metabolic capabilities including anoxygenic photosynthesis, aerobic or anaerobic respiration, and fermentation. Growth is achieved by using H{sub 2} as an electron donor and CO{sub 2} as a carbon source during photoautotrophic and chemoautotrophic growth, where CO{sub 2} is fixed via the Calvin-Benson-Bassham (CBB) cycle. Photoheterotrophic growth can also occur when alternative organic carbon compounds are utilized as both the carbon source and electron donor. Regardless of the growth mode, excess reducing equivalents generated as a result of oxidative processes, must be transferred to terminal electron acceptors, thus insuring that redox homeostasis is maintained in the cell. Possible terminal acceptors include O{sub 2}, CO{sub 2}, organic carbon, or various oxyanions. Cells possess regulatory mechanisms to balance the activity of the pathways which supply energy, such as photosynthesis, and those that consume energy, such as CO{sub 2} assimilation or N{sub 2} fixation. The major route for CO{sub 2} assimilation is the CBB reductive pentose phosphate pathway, whose key enzyme is ribulose 1,5-biphosphate carboxylase/oxygenase (RubisCO). In addition to providing virtually all cellular carbon during autotrophic metabolism, RubisCO-mediated CO{sub 2} assimilation is also very important for nonsulfur purple photosynthetic bacteria under photoheterotrophic growth conditions since CO{sub 2} becomes the major electron sink under these conditions. In this work, Ensemble Modeling (EM) was developed to examine the behavior of CBB-compromised RubisCO knockout mutant strains of the nonsulfur purple photosynthetic bacterium Rhodobacter sphaeroides. Mathematical models of metabolism can be a great aid in studying the effects of large perturbations to the system, such as the inactivation of RubisCO. Due to the complex and highly-interconnected nature of these networks, it is not a trivial process to understand what the effect of perturbations to the metabolic network will be, or vice versa, what enzymatic perturbations are necessary to yield a desired effect. Flux distribution is controlled by multiple enzymes in the network, often indirectly linked to the pathways of interest. Further, depending on the state of the cell and the environmental conditions, the effect of a perturbation may center around how it effects the carbon flow in the network, the balancing of cofactors, or both. Thus, it is desirable to develop mathematical models to describe, understand, and predict network behavior. Through the development of such models, one may gain the ability to generate a set of testable hypotheses for system behavior.

James C. Liao

2012-05-22T23:59:59.000Z

30

A combined microfluidic/dielectrophoretic microorganism concentrator  

E-Print Network [OSTI]

This thesis presents the development of a high-throughput microfluidic microorganism concentrator for pathogen detection applications. Interdigitated electrodes lining the bottom of the channel use positive dielectrophoretic ...

Gadish, Nitzan

2005-01-01T23:59:59.000Z

31

Onsite Wastewater Treatment Systems: Aerobic Treatment Unit  

E-Print Network [OSTI]

wastewater treatment systems use. They remove 85 to 98 percent of the organic matter and solids from the wastewater, producing effluent as clean as that from munici- pal wastewater treatment plants, and cleaner than that from conventional septic tanks.... Onsite wastewater treatment systems Single-compartment trash tank Chlorinator Aerobic treatment unit Spray heads Pump tank Bruce Lesikar Professor and Extension Agricultural Engineer The Texas A&M System Aerobic treatment units, which are certified...

Lesikar, Bruce J.

2008-10-31T23:59:59.000Z

32

Oil Production by a Consortium of Oleaginous Microorganisms grown on primary effluent wastewater  

SciTech Connect (OSTI)

Municipal wastewater could be a potential growth medium that has not been considered for cultivating oleaginous microorganisms. This study is designed to determine if a consortium of oleaginous microorganism can successfully compete for carbon and other nutrients with the indigenous microorganisms contained in primary effluent wastewater. RESULTS: The oleaginous consortium inoculated with indigenous microorganisms reached stationary phase within 24 h, reaching a maximum cell concentration of 0.58 g L -1. Water quality post-oleaginous consortium growth reached a maximum chemical oxygen demand (COD) reduction of approximately 81%, supporting the consumption of the glucose within 8 h. The oleaginous consortium increased the amount of oil produced per gram by 13% compared with indigenous microorganisms in raw wastewater. Quantitative polymerase chain reaction (qPCR) results show a substantial population increase in bacteria within the first 24 h when the consortium is inoculated into raw wastewater. This result, along with the fatty acid methyl esters (FAMEs) results, suggests that conditions tested were not sufficient for the oleaginous consortium to compete with the indigenous microorganisms.

Hall, Jacqueline; Hetrick, Mary; French, Todd; Hernandez, Rafael; Donaldson, Janet; Mondala, Andro; Holmes, William

2011-01-01T23:59:59.000Z

33

Cellulase producing microorganism ATCC 55702  

DOE Patents [OSTI]

Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

Dees, H.C.

1997-12-30T23:59:59.000Z

34

Cellulase producing microorganism ATCC 55702  

DOE Patents [OSTI]

Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

Dees, H. Craig (Lenoir City, TN)

1997-01-01T23:59:59.000Z

35

E-Print Network 3.0 - aerobic reactions abbau Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

supported by ei- ther aerobic or anaerobic metabolism. Aerobic... demand and aerobic energy supply. In addition, each functional system in- volved in oxygen ... Source: Bennett,...

36

Emerging contaminants and microorganisms into the environment  

E-Print Network [OSTI]

Workshop Emerging contaminants and microorganisms into the environment: contamination pathways Environmental Engineering Division Surname _____________________Name____________________ Job Environmental Engineering Division (SEED) Department of Civil Engineering University of Salerno Via Ponte don

Costagliola, Gennaro

37

A microfabricated dielectrophoretic micro-organism concentrator  

E-Print Network [OSTI]

This project focuses on the development of a micro-organism concentrator. Pathogen detection, particularly MEMS based detection, is often limited by sample concentration. The proposed concentrator will interface with a ...

Muller, Rikky, 1980-

2004-01-01T23:59:59.000Z

38

AEROBIC BIOTRANSFORMATION OF TOXIC ORGANICS IN WASTEWATER  

E-Print Network [OSTI]

#12;AEROBIC BIOTRANSFORMATION OF TOXIC ORGANICS IN WASTEWATER DOE FRAP 1997-15 Prepared for in both domestic and industrial wastewater. The release of these compounds during wastewater treatment to predict the mass of the VOCs in the wastewater treated by biotransformation and the mass stripped

39

Bacteria isolated from amoebae/bacteria consortium  

DOE Patents [OSTI]

New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

Tyndall, Richard L. (Clinton, TN)

1995-01-01T23:59:59.000Z

40

Effects of microorganisms growth on the long-term stability of cement and bitumen  

SciTech Connect (OSTI)

Cement is used as a coating matrix for nuclear waste or as an engineered barrier of waste repositories situated in geological formations. The effect of mineral acids excreted by bacteria (Thiobacillus) or organic acids produced by fungi, on the biodegradation of cement is discussed. Organic acids are quantitatively and qualitatively determined during growth of fungi over a two-year period. Even with high pH conditions, pH of the cement {approx} 11, growth of microorganisms occurs. Biodeterioration of cement is expressed in terms of bioleaching velocity of calcium and is observed by electron microscopy. Bitumen is commonly used as a matrix for the long-term storage of radioactive wastes. Long-term biodegrability of bitumen is discussed as a function of its chemical composition and various studied microorganisms.

Libert, M.F.; Sellier, R.; Jouquet, G.; Trescinski, M.; Spor, H. [Nuclear Research Center of Cadarache, St.Paul-Lez-Durance (France)

1993-12-31T23:59:59.000Z

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

Recombinant microorganisms for increased production of organic acids  

DOE Patents [OSTI]

Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

Yi, Jian; Kleff, Susanne; Guettler, Michael V

2013-04-30T23:59:59.000Z

42

Recombinant microorganisms for increased production of organic acids  

DOE Patents [OSTI]

Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

Yi, Jian (East Lansing, MI); Kleff, Susanne (East Lansing, MI); Guettler, Michael V. (Holt, MI)

2012-02-21T23:59:59.000Z

43

aerobic anoxygenic photosynthesis: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and belowground production, and litterfall; (2) aerobic and anaerobic decomposition of peat; (3) production, oxidation was generally biased toward net ecosystem respiration...

44

aerobic anoxygenic phototrophy: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and electricity producing using MFCs in order to achieve both wastewater treatment and bioenergy). Aerobic wastewater treatment costs can be very high for these wastewaters, and...

45

aerober mikrobieller abbau: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and electricity producing using MFCs in order to achieve both wastewater treatment and bioenergy). Aerobic wastewater treatment costs can be very high for these wastewaters, and...

46

aerobic phototrophic alphaproteobacterium: Topics by E-print...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

capacity than uninfected males, but only for small males. Discriminant function analysis suggested that aerobic capacity and display behaviour, together with male ornaments...

47

aerobic anoxygenic phototrophic: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

capacity than uninfected males, but only for small males. Discriminant function analysis suggested that aerobic capacity and display behaviour, together with male ornaments...

48

aerobic anoxygenic phototrophs: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

capacity than uninfected males, but only for small males. Discriminant function analysis suggested that aerobic capacity and display behaviour, together with male ornaments...

49

Formation of aerobic granular sludge biofilms for sustainable wastewater treatment  

E-Print Network [OSTI]

ENAC/ Formation of aerobic granular sludge biofilms for sustainable wastewater treatment David G Research, Microbiology of Interfaces, Magdeburg (Germany) EDCE 2011 / From activated sludge flocs

50

aerobic bacterial consortium: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

biology of its aerobic physiology is well studied. Our deer mice grew throughout life, and, as is typical for mammals, their basal metabolic rate (BMR) and maximal oxygen...

51

Electric DNA chips for determination of pathogenic microorganisms  

E-Print Network [OSTI]

Electric DNA chips for determination of pathogenic microorganisms Yanling Liu Doctoral thesis Liu (2008): Electric DNA chips for determination of pathogenic microorganisms. School of Biotechnology, Royal Institute of Technology (KTH), Stockholm, Sweden Abstract Silicon-based electric DNA chip arrays

Enfors, Sven-Olof

52

aerobic energy turnover: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

aerobic energy turnover First Page Previous Page 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 Misconceptions about Aerobic...

53

E-Print Network 3.0 - aerobic respiratory chain Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

with the supe- rior mass-specific aerobic function of these muscles. aerobic metabolism; capillary; experimental... evolution; selective breed- ing; skeletal muscle THE MAXIMAL...

54

E-Print Network 3.0 - aerobic exercise capacity Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

to the aerobic capacity of skeletal muscles (21, 25, 50). Histori- cally, a denser capillary... from animals adapted for high aerobic capacity, ... Source: Garland Jr.,...

55

E-Print Network 3.0 - aerobic efficiency measuring Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Method for Determining the Aerobic Biodegradation of Plastic Materials in the Presence of Municipal... Sewage Sludge D 5209 2. Standard Test Method for Determining the Aerobic...

56

aerobic capacity co-segregates: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

methyl-triclosan in activated sludge under aerobic Keywords: Triclosan Triclosan methyl Waste water treatment Aerobic transformation a b s t r a c t Triclosan (WWTPs) and...

57

E-Print Network 3.0 - aerobic tellurite-resistant marine Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

MA 01930... incubation at 20e. For the aerobic plate count, standard methods agar was fortified with 5 g bacto peptone... and Discussion The aerobic plate counts (APC)...

58

E-Print Network 3.0 - aerobic physical activity Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

activity whereby har- vesting led to decreased aerobic and anaerobic CO2 production rates, presumably due... for carbon dioxide (CO2) production under aerobic and...

59

E-Print Network 3.0 - aerobic kinetic resolution Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

(MAS, maximal aerobic speed). Temperature affected the kinematics... consumption; MAS maximum aerobic speed; C i, minimum cost of locomotion; te,,endurance time * To whom... -...

60

E-Print Network 3.0 - aerobic heterotrophic bacteria Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

utilise 10to 50% of carbon fixed by ... Source: Vallino, Joseph J. - Ecosystems Center, Marine Biological Laboratory, Woods Hole Collection: Environmental Sciences and...

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

Bacteria-Mineral Interactions on the Surfaces of Metal-Resistant Bacteria  

SciTech Connect (OSTI)

The extraordinary ability of indigenous microorganisms, like metal-resistant bacteria, for biotransformation of toxic compounds is of considerable interest for the emerging area of environmental bioremediation. However, the underlying mechanisms by which metal-resistant bacteria transform toxic compounds are currently unknown and await elucidation. The project's objective was to study stress-induced responses of metal-resistant bacteria to environmental changes and chemical stimulants. This project involved a multi-institutional collaboration of our LLNL group with the group of Dr. H.-Y. Holman (Lawrence Berkeley National Laboratory). In this project, we have utilized metal-resistant bacteria Arthrobacter oxydans as a model bacterial system. We have utilized atomic force microscopy (AFM) to visualize for the first time at the nanometer scale formation of stress-induced structures on bacterial surfaces in response to Cr (VI) exposure. We have demonstrated that structure, assembly, and composition of these stress-induced structures are dependent on Cr (VI) concentrations. Our AFM observations of the appearance and development of stress-induced layers on the surfaces of Arthrobacter oxydans bacteria exposed to Cr (VI) were confirmed by Dr. Holman's biochemical, electron microscopy, and synchrotron infrared spectromicroscopy studies. In general, in vitro imaging of live microbial and cellular systems represents one of the most challenging issues in application of AFM. Various approaches for immobilization of bacteria on the substrate for in vitro imaging were tested in this project. Imaging of live bacteria was achieved, however further optimization of experimental methods are needed for high-resolution visualization of the cellular environmental structural dynamics by AFM. This project enhanced the current insight into molecular architecture, structural and environmental variability of bacterial systems. The project partially funded research for two book chapters (1,2), and we anticipate one more publication (3). The publications describe development of methods and results of studies of structural dynamics of metal-resistant bacteria that contribute to more comprehensive understanding of the architecture, function, and environmental dynamics of bacterial and cellular systems. The results of this LDRD were presented in invited talks and contributed presentations at five national and international conferences and five seminar presentations at the external institutions. These included invited talks at the conferences of Gordon Research, Materials Research and American Chemical Societies. Our scientific results and methodologies developed in this project enabled us to receive new funding for the multiyear project 'Chromium transformation pathways in metal-reducing bacteria' funded by the University of California Lab Fees Program ($500,000, 5/1/09 - 4/30/2012), with our proposal being ranked 1st from a total of 138 in the Earth, Energy, Environmental & Space Sciences panel.

Malkin, A J

2010-03-24T23:59:59.000Z

62

Bacteria in shear flow  

E-Print Network [OSTI]

Bacteria are ubiquitous and play a critical role in many contexts. Their environment is nearly always dynamic due to the prevalence of fluid flow: creeping flow in soil, highly sheared flow in bodily conduits, and turbulent ...

Marcos, Ph.D. Massachusetts Institute of Technology

2011-01-01T23:59:59.000Z

63

aerobic bacterium tetrathiobacter: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

H. Kwan; We Thank J. Decruyenaere; C. Hayes; T. Kim; T. Maclarty; S. Popma For Their 1997-01-01 10 Mate choice and aerobic capacity in red junglefowl CiteSeer Summary: In 'good...

64

Aerobic Glycolysis: Meeting the Metabolic Requirements of Cell Proliferation  

E-Print Network [OSTI]

Warburg's observation that cancer cells exhibit a high rate of glycolysis even in the presence of oxygen (aerobic glycolysis) sparked debate over the role of glycolysis in normal and cancer cells. Although it has been ...

Vander Heiden, Matthew G.

65

Apparatus and method for the desulfurization of petroleum by bacteria  

DOE Patents [OSTI]

A method for treating petroleum with anaerobic microorganisms acting as biocatalysts that can remove sulfur atoms from hydrocarbon molecules, under anaerobic conditions, and then convert the sulfur atoms to hydrogen sulfide. The microorganisms utilized are from the family known as the "Sulfate Reducing Bacteria." These bacteria generate metabolic energy from the oxidation of organic compounds, but use oxidized forms of sulfur as an electron acceptor. Because the biocatalyst is present in the form of bacteria in an aqueous suspension, whereas the reacting substrate consists of hydrocarbon molecules in an organic phase, the actual desulfurization reaction takes place at the aqueous-organic interphase. To ensure adequate interfacial contacting and mass transfer, a biphasic electrostatic bioreactor system is utilized. The bioreactor is utilized to disperse and recoalesce a biocatalyst contained in the aqueous liquid phase into the organic liquid phase containing the sulfur. High-intensity electrical fields rupture the aqueous drops into a plurality of microdroplets and induce continuous coalescence and redispersion as the microdroplets travel through the organic phase, thus increasing surface area. As the aqueous microdroplets progress through the organic phase, the biocatalyst then reacts with the sulfur to produce hydrogen sulfide which is then removed from the bioreactor. The organic liquid, now free of the sulfur, is ready for immediate use or further processing.

Lizama, Hector M. (Knoxville, TN); Scott, Timothy C. (Knoxville, TN); Scott, Charles D. (Oak Ridge, TN)

1995-01-01T23:59:59.000Z

66

Apparatus and method for the desulfurization of petroleum by bacteria  

DOE Patents [OSTI]

A method is described for treating petroleum with anaerobic microorganisms acting as biocatalysts that can remove sulfur atoms from hydrocarbon molecules, under anaerobic conditions, and then convert the sulfur atoms to hydrogen sulfide. The microorganisms utilized are from the family known as the ``Sulfate Reducing Bacteria``. These bacteria generate metabolic energy from the oxidation of organic compounds, but use oxidized forms of sulfur as an electron acceptor. Because the biocatalyst is present in the form of bacteria in an aqueous suspension, whereas the reacting substrate consists of hydrocarbon molecules in an organic phase, the actual desulfurization reaction takes place at the aqueous-organic interphase. To ensure adequate interfacial contacting and mass transfer, a biphasic electrostatic bioreactor system is utilized. The bioreactor is utilized to disperse and recoalesce a biocatalyst contained in the aqueous liquid phase into the organic liquid phase containing the sulfur. High-intensity electrical fields rupture the aqueous drops into a plurality of microdroplets and induce continuous coalescence and redispersion as the microdroplets travel through the organic phase, thus increasing surface area. As the aqueous microdroplets progress through the organic phase, the biocatalyst then reacts with the sulfur to produce hydrogen sulfide which is then removed from the bioreactor. The organic liquid, now free of the sulfur, is ready for immediate use or further processing. 5 figs.

Lizama, H.M.; Scott, T.C.; Scott, C.D.

1995-10-17T23:59:59.000Z

67

Evaluation of terrestrial microcosms for assessing the fate and effects of genetically engineered microorganisms on ecological processes  

SciTech Connect (OSTI)

This project evaluates and modifies the existing US Environmental Protection Agency's Office of Pesticides and Toxic Substances (EPA/OPTS) terrestrial microcosm test system and test protocols such that they can be used to determine the environmental fate and ecological hazards of genetically engineered microorganisms (GEMs). The intact soil-core microcosm represents terrestrial ecosystems, and when coupled with appropriate test protocols, such microcosms may be appropriate to define and limit risks associated with the intentional release of GEMs. The terrestrial microcosm test system was used to investigate the survival and transport of two model GEMs (Azospirillum lipoferum and Pseudomonas sp. Tn5 mutants) to various trophic levels and niches and through intact soil cores. Subsequent effects on nutrient cycling and displacement of indigenous microorganisms were evaluated. The model organisms were a diazotrophic root-colonizing bacterium (A. lipoferum) and a wheat root growth-inhibiting rhizobacterium (Pseudomonas sp.). The transposable element Tn5 was used as a genetic marker for both microorganisms in two separate experiments. The organisms were subjected to transposon mutagenesis using a broad host-range-mobilizable suicide plasmid. The transposon Tn5 conferred levels of kanamycin resistance up to 500 ..mu..g/ml (Pseudomonas sp.), which allowed for selection of the bacteria from environmental samples. The presence of Tn5 DNA in the genome of the model GEMs also allowed the use of Tn5 gene probes to confirm and enumerate the microorganisms in different samples from the microcosms. Two types of root growth-inhibiting Pseudomonas sp. Tn5 mutants were obtained and used in microcosm studies: those that lacked the ability to inhibit either wheat root growth or the growth of other microorganisms in vitro (tox/sup /minus//) and those which retained these properties (tox/sup +/). 53 refs., 7 figs., 6 tabs.

Fredrickson, J.K.; Bentjen, S.A.; Bolton, H. Jr.; Li, S.W.; Ligotke, M.W.; McFadden, K.M.; Van Voris, P.

1989-04-01T23:59:59.000Z

68

Laboratory and Field Evidence for Long-Term Starvation Survival of Microorganisms in Subsurface Terrestrial Environments  

SciTech Connect (OSTI)

BIOGEOCHEMICAL MODELING OF GROUNDWATER FLOW AND NUTRIENT FLUX IN SUBSURFACE ENVIRONMENTS INDICATES THAT INHABITANT MICROORGANISMS EXPERIENCE SEVERE NUTRIENT LIMITATION. USING LABORATORY AND FIELD METHODS, WE HAVE BEEN TESTING STARVATION SURVIVAL IN SUBSURFACE MICROORGANISMS. IN MICROCOSM EXPERIMENTS, WE HAVE SHOWN THAT STRAINS OF TWO COMMONLY ISOLATED SUBSURFACE GENERA, ARTHROBACTER AND PSEUDOMONAS, ARE ABLE TO MAINTAIN VIABILITY IN LOW-NUTRIENT, NATURAL SUBSURFACE SEDIMENTS FOR OVER ONE YEAR. THESE NON-SPORE-FORMING BACTERIA UNDERGO RAPID INITIAL MINIATURIZATION FOLLOWED BY A STABILIZATION OF CELL SIZE. MEMBRANE LIPID PHOSPHOLIPID FATTY ACID (PLFA) PROFILES OF THE PSEUDOMONAS ARE CONSISTENT WITH ADAPTATION TO NUTRIENT STRESS; ARTHROBACTER APPARENTLY RESPONDS TO NUTRIENT DEPRIVATION WITHOUT ALTERING MEMBRANE PLFA. TO TEST SURVIVABILITY OF MICROORGANISMS OVER A GEOLOGIC TIME SCALE, WE CHARACTERIZED MICROBIAL COMMUNITIES IN A SEQUENCE OF UNSATURATED SEDIMENTS RANGING IN AGE FROM MODEM TO {gt}780,000 years. Sediments were relatively uniform silts in Eastern Washington State. Porewater ages at depth (measured by the chloride mass-balance approach) were as old as 3,600 years. Microbial abundance, biomass, and activities (measured by direct counts, culture counts, total PLFAs, and radiorespirometry) declined with sediment age. The pattern is consistent with laboratory microcosm studies of Microbial survival: rapid short-term change followed by long-term survival of a proportion of cells. Even the oldest sediments evinced a small but viable Microbial community. Microbial survival appeared to be a function of sediment age. Porewater age appeared to influence the markup of surviving communities, as indicated by PLFA profiles. Sites with different Porewater recharge rates and patterns of Pleistocene flooding had different communities.

Kieft, T.L. [Biology Dept., New Mexico Inst. of Mining and Technology, Socorro, NM (United States); Murphy, E.M. [Pacific Northwest National Lab., Richland, WA (United States); Amy, P.S.; Haldeman, D.L. [Department of Biological Sciences, University of Nevada, Las Vegas, NV (United States); Ringelberg, D. B. [Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN (United States)]|[Oak Ridge National Lab., TN (United States)

1997-12-31T23:59:59.000Z

69

Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism  

DOE Patents [OSTI]

A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H.sub.2 O and/or CO.sub.2 and H.sub.2 in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate.

Gaddy, James L. (Fayetteville, AR); Clausen, Edgar C. (Fayetteville, AR)

1992-01-01T23:59:59.000Z

70

Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism  

DOE Patents [OSTI]

A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H[sub 2]O and/or CO[sub 2] and H[sub 2] in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate. 3 figs.

Gaddy, J.L.; Clausen, E.C.

1992-12-22T23:59:59.000Z

71

E-Print Network 3.0 - aerobe und anaerobe Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

of GI, tissue, cavity or wound anaerobes. Aerobic and fungal... to avoid loss of oxygen-excluding gas cap. Blood Culture Bottles for Aerobic and Anaerobic Culture... are used...

72

E-Print Network 3.0 - aerobic biological processes Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

area's trenches or beds, and (2) on the top surface of an aerobic... to receive wastewater and be maintained in an oxygen rich (aerobic) condition, have become wet and oxygen...

73

Abiotic Aerobic Methane Release from Plant Material Dan Bruhn, Per Ambus & Teis N Mikkelsen  

E-Print Network [OSTI]

Abiotic Aerobic Methane Release from Plant Material Dan Bruhn, Per Ambus & Teis N Mikkelsen)1 reported a new finding of aerobic methane (CH4) release by living plant and even dead tissue. Keppler et al

74

Selective microorganism concentration using a dielectrophoresis-based microfabricated device  

E-Print Network [OSTI]

Detection of pathogenic microorganisms is a significant challenge in medicine, environmental protection and biological threat safety because samples are often contaminated. This work presents a method of separating bacterial ...

Pucha?a, Katarzyna Anna

2007-01-01T23:59:59.000Z

75

Microorganisms having enhanced resistance to acetate and methods of use  

SciTech Connect (OSTI)

The present invention provides isolated or genetically modified strains of microorganisms that display enhanced resistance to acetate as a result of increased expression of a sodium proton antiporter. The present invention also provides methods for producing such microbial strains, as well as related promoter sequences and expression vectors. Further, the present invention provides methods of producing alcohol from biomass materials by using microorganisms with enhanced resistance to acetate.

Brown, Steven D; Yang, Shihui

2014-10-21T23:59:59.000Z

76

Microorganisms having enhanced tolerance to inhibitors and stress  

DOE Patents [OSTI]

The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.

Brown, Steven D.; Yang, Shihui

2014-07-29T23:59:59.000Z

77

Bioengineering and Coordination of Regulatory Networks and Intracellular Complexes to Maximize Hydrogen Production by Phototrophic Microorganisms  

SciTech Connect (OSTI)

In this study, the Principal Investigator, F.R. Tabita has teemed up with J. C. Liao from UCLA. This project's main goal is to manipulate regulatory networks in phototrophic bacteria to affect and maximize the production of large amounts of hydrogen gas under conditions where wild-type organisms are constrained by inherent regulatory mechanisms from allowing this to occur. Unrestrained production of hydrogen has been achieved and this will allow for the potential utilization of waste materials as a feed stock to support hydrogen production. By further understanding the means by which regulatory networks interact, this study will seek to maximize the ability of currently available “unrestrained” organisms to produce hydrogen. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Moreover, due to their great metabolic versatility, such organisms highly regulate these processes in the cell and since virtually all such capabilities are dispensable, excellent experimental systems to study aspects of molecular control and biochemistry/physiology are available.

Tabita, F. Robert [The Ohio State University] [The Ohio State University

2013-07-30T23:59:59.000Z

78

Beyond Bacteria | EMSL  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth (AOD)ProductssondeadjustsondeadjustAboutScienceCareers Apply for a JobBernardthe AlcatorBeverlyBeyond Bacteria

79

Evaluation of terrestrial microcosms for detection, fate, and survival analysis of genetically engineered microorganisms and their recombinant genetic material  

SciTech Connect (OSTI)

The research included in this document represents the current scientific information available regarding the applicability of terrestrial microcosms and related methodologies for evaluating detection methods and the fate and survival of microorganisms in the environment. The three terrestrial microcosms described in this document were used to evaluate the survival and fate of recombinant bacteria in soils and in association with plant surfaces and insects and their transport through soil with percolating water and root systems, and to test new methods and procedures to improve detection and enumeration of bacteria in soil. Simple (potting soil composed of peat mix and perlite, lacking environmental control and monitoring) and complex microcosms (agricultural soil with partial control and monitoring of environmental conditions) were demonstrated to be useful tools for preliminary assessments of microbial viability in terrestrial ecosystems. These studies evaluated the survival patterns of Enterobacter cloacae (pBR322) in soil and on plant surfaces and the ingestion of this same microorganism by cutworms and survival in the foregut and frass. The Versacore microcosm design was used to monitor the fate and competitiveness of genetically engineered bacteria in soil. Both selective media and gene probes were used successfully to follow the fate of two recombinant Pseudomonas sp. introduced into Versacore microcosms. Intact soil-core microcosms were employed to evaluate the fate and transport of genetically altered Azospirillum sp. and Pseudomonas sp. in soil and the plant rhizosphere. The usefulness of these various microcosms as a tool for risk assessment is underscored by the ease in obtaining soil from a proposed field release site to evaluate subsequent GEM fate and survival.

Fredrickson, J.K.; Seidler, R.J.

1989-02-01T23:59:59.000Z

80

Effects of heavy metals on microorganisms application to process design  

E-Print Network [OSTI]

of Domestic Sewage. " Water Pollution Control, 1970, pp. 100-110. 3. Banerji, Shankha K. , discussion of "Toxicity Measurements in Activated Sludge. " by L. Hartmann and G, Laubenberger, Journal of the Sanitar En ineerin Division, ASCE, Vol. 95, No. SA4... in Aerobic Biolo ical Treatment of Waste Water Princi le and Practice. Oligodynamic Press, Houston, Texas, 1971, p. 130. 10. Hartmann, Ludwig, and Laubenberger, G. , "Toxicity Measure- ments in Activated Sludge. " Journal of the Sanita En i- neerin...

Heck, Robert Paul

1972-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

E-Print Network 3.0 - aerobic fixed bed Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Refuse Derived Fuel (RDF) incineration. The objective... . The overall plant consists of MSW reception , separation and recycling, aerobic composting of the organic... the...

82

E-Print Network 3.0 - aerobic yeast yarrowia Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

search results for: aerobic yeast yarrowia Page: << < 1 2 3 4 5 > >> 1 Evolutionary Genomics: Yeasts Accelerate beyond BLAST Summary: hansenii, and Yarrowia lipolytica. Yeast 20,...

83

Mechanism of the Aerobic Oxidation of Alcohols by Palladium Complexes of N-Heterocyclic Carbenes  

E-Print Network [OSTI]

reductive elimination of acetic acid, which yields a slightly higher calculated activation barrier-catalyzed aerobic oxidations of benzylic and allylic secondary alcohols, routinely fostering selectivity factors (k

Goddard III, William A.

84

Synthesis of poly-(P-aryleneethynylene)s in neat water under aerobic conditions  

DOE Patents [OSTI]

Provided are ethyne synthons comprising boron and related methods. Also provided are related water-soluble arylethynylene polymers capable of being synthesized in neat water under aerobic conditions.

Kang, Youn K; Deria, Pravas; Therien, Michael J

2012-10-16T23:59:59.000Z

85

E-Print Network 3.0 - aerobic hypolimnia relative Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Topic List Advanced Search Sample search results for: aerobic hypolimnia relative Page: << < 1 2 3 4 5 > >> 1 Biogeochemistry 38: 281301, 1997. c 1997 Kluwer Academic...

86

E-Print Network 3.0 - aerobic deterioration influences Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

is a quantitative trait influenced... ; thus, axiomatically the efficiency of aerobic energy transfer is pivotal to cellular metabolism... by the inter- action of multiple genetic...

87

Geobiology of marine magnetotactic bacteria  

E-Print Network [OSTI]

Magnetotactic bacteria (MTB) biomineralize intracellular membrane-bound crystals of magnetite (Fe3O4) or greigite (Fe3S4), and are abundant in the suboxic to anoxic zones of stratified marine environments worldwide. Their ...

Simmons, Sheri Lynn

2006-01-01T23:59:59.000Z

88

Prokaryotic microorganisms in uranium mining waste piles and their interactions with uranium and other heavy metals.  

E-Print Network [OSTI]

??The influence of uranyl and sodium nitrate under aerobic and anaerobic conditions on the microbial community structure of a soil sample from the uranium mining… (more)

Geißler, Andrea

2007-01-01T23:59:59.000Z

89

Ecovation Inc formerly AnAerobics | Open Energy Information  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 NoPublic Utilities Address:011-DNA Jump37. It is classified asThisEcoGrid EU (SmartEcovation Inc formerly AnAerobics

90

2004 Sensory Transduction in Microorganisms Gordon Research Conference-January 11-16, 2004  

SciTech Connect (OSTI)

Research into the mechanisms involved in the sensing and responses of microorganisms to changes in their environment is currently very active in a large number of laboratories in the US, Europe, Japan, and Israel. A wide range of eukaryotic and prokaryotic species are being studies with regard to their sensing of chemical changes, light and redox signal and intercellular signaling, leading either to changes in motile behavior, gene expression or development. It has become increasingly apparent that the mechanisms involved in development have application in higher organisms while the sensing systems in bacteria are involved in a very wide range of physiological traits, from pathogenicity, through to biofilm formation. This is an area where a wide range of state of the art tools have been used and developed over the past few decades. Approaches include behavioral studies, electro-physiology, genetics, molecular biology, structural biology, biophysics and single molecule microscopy, immunocytochemistry and molecular and mathematical modeling, all of this helped by the large number of bacterial and eukaryotic microbial genome sequences now available. The central goal of this meeting is to bring together investigators using this wide range of approaches and different systems to compare data, share ideas and approaches and seeks to understand the fundamental principles underlying these responses.

Judith Armitage Carlyle Storm

2005-01-07T23:59:59.000Z

91

Studies on the activities of rumen microorganisms in vitro  

E-Print Network [OSTI]

to the functional state of the rumen. The methods used by various investigators for measuring rumen microbial activity includei (a) cellulose digestion, (b) utilization of non-protein nitrogen, (c) production of volatile fatty acids, (d) production of carbon... EXTRACTS ON THE DIGESTION OF CELLULOSE BE RUMEN MICROORGANISMS INTRODUCTION Page Review of Literature Collection of Numen Samples Processing of Rumen Samples Measurement of the Activity of Rurren Yiicroorgan~ s'". s Procedure for ~vt-, c Incubation...

Johnson, Carl Edward

1957-01-01T23:59:59.000Z

92

Location and chemical composition of microbially induced phosphorus precipitates in anaerobic and aerobic granular sludge  

E-Print Network [OSTI]

and aerobic granular sludge A. Mañasa,b,c,e , M. Spérandioa,b,c , F. Deckerd and B. Biscanse aINSA, UPS, INP X-ray analysis (SEM-EDX) applied to granular sludge used for biological treatment of high sludge blanket (UASB) reactors and two aerobic granular sequenced batch reactors (GSBR) were evaluated

Boyer, Edmond

93

Studies of Copper Nanoparticles Effects on Micro-organisms  

E-Print Network [OSTI]

We discuss about the antibacterial activities of copper nanoparticles on both Gram negative and Gram positive bacteria in this investigation. First time, we increase its antibacterial activities by using electrical power while on electrolysis synthesis and it is confirmed from its more antibacterial activities (For Escherichia coli bacteria). We investigate the changes of surface area to volume ratio of copper nanoparticles prepared in two different methods and its effects on antibacterial activities. We note that slight change of surface area to volume ratio results in the enhancement of its antibacterial activities.

T. Theivasanthi; M. Alagar

2011-10-05T23:59:59.000Z

94

A laboratory study of the activities of dried rumen microorganisms  

E-Print Network [OSTI]

. 01736 gm. /ml. By all of the criteria used, (except the digestion of carboxyzethyl cellulose) the activities of dried rumen preparations were very much lover than those of fresh rumen liquid in the "artificial rumen". This may be attributed to nne... have little or no ~ ~ act1vity vith respect to cellulose digestion, non-protein nitrogen utilisation, or vitamin synthesis. The failure of the bacteria to be reactivated may bs due to one or more of the following possible causes& (s) the bacteria...

Harbers, Leniel Henry

1958-01-01T23:59:59.000Z

95

DEVELOPMENT OF MICROORGANISMS WITH IMPROVED TRANSPORT AND BIOSURFACTANT ACTIVITY FOR ENHANCED OIL RECOVERY  

SciTech Connect (OSTI)

Diverse microorganisms were screened for biosurfactant production and anaerobic growth at elevated salt concentrations to obtain candidates most suitable for microbial oil recovery. Seventy percent of the 205 strains tested, mostly strains of Bacillus mojavensis, Bacillus subtilis, Bacillus licheniformis, and Bacillus sonorensis, produced biosurfactants aerobically and 41% of the strains had biosurfactant activity greater than Bacillus mojavensis JF-2, the current candidate for oil recovery. Biosurfactant activity varied with the percentage of the 3-hydroxy-tetradecanoate isomers in the fatty acid portion of the biosurfactant. Changing the medium composition by incorporation of different precursors of 3-hydroxy tetradecanoate increased the activity of biosurfactant. The surface tension and critical micelle concentration of 15 different, biosurfactant-producing Bacillus strains was determined individually and in combination with other biosurfactants. Some biosurfactant mixtures were found to have synergistic effect on surface tension (e.g. surface tension was lowered from 41 to 31 mN/m in some cases) while others had a synergistic effect on CMD-1 values. We compared the transport abilities of spores from three Bacillus strains using a model porous system to study spore recovery and transport. Sand-packed columns were used to select for spores or cells with the best transport abilities through brine-saturated sand. Spores of Bacillus mojavensis strains JF-2 and ROB-2 and a natural recombinant, strain C-9, transported through sand at very high efficiencies. The earliest cells/spores that emerged from the column were re-grown, allowed to sporulate, and applied to a second column. This procedure greatly enhanced the transport of strain C-9. Spores with enhanced transport abilities can be easily obtained and that the preparation of inocula for use in MEOR is feasible. Tertiary oil recovery experiments showed that 10 to 40 mg/l of JF-2 biosurfactant in the presence of 0.1 mM 2,3-butanediol and 1 g/l of partially hydrolyzed polyacrylamide (PHPA) recovered 10-40% of residual oil from Berea sandstone cores. When PHPA was used alone, about 10% of the residual oil was recovered. Interfacial tension (IFT) decreased in a stepwise manner as biosurfactant concentration increased with marked reductions in IFT occurring at biosurfactant concentrations of 10 and 40 mg/l. When the biosurfactant concentration was greater than 10 mg/l, residual oil recovery linearly increased with biosurfactant concentration. A mathematical model that relates oil recovery to biosurfactant concentration was modified to include the stepwise changes in IFT as biosurfactant concentrations changes. This model adequately predicted the experimentally observed changes in IFT as a function of biosurfactant concentration. Our work shows that (1) diverse microorganisms produce biosurfactants, (2) nutrient manipulation may provide a mechanism to increase biosurfactant activity, (3) biosurfactant concentrations in excess of the critical micelle concentration recover substantial amounts of residual oil, and (4) equations that describe the effect of the biosurfactant on IFT adequately predict residual oil recovery in sandstone cores.

M.J. McInerney; N. Youssef; T. Fincher; S.K. Maudgalya; M.J. Folmsbee; R. Knapp; D. Nagle

2004-05-31T23:59:59.000Z

96

Microorganisms to Speed Production of Biofuels - Energy Innovation Portal  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: VegetationEquipment Surfaces andMapping theEnergyInnovationMichaelGE ResearchersIndustrialMicroorganisms

97

Effects of remediation amendments on vadose zone microorganisms  

SciTech Connect (OSTI)

Surfactant-based foam delivery technology has been studied to remediate Hanford 200 area deep vadose zone sediment. However, the surfactants and remediation amendments have an unknown effect on indigenous subsurface microorganisms. Microbial populations are important factors to consider in remediation efforts due to their potential to alter soil geochemistry. This project focuses on measuring microbial metabolic responses to remediation amendments in batch and column studies using Deep Vadose Zone Sediments. Initial studies of the microbes from Hanford 200 area deep vadose zone sediment showed surfactants sodium dodecyl sulfate (SDS) and cocamidopropyl betaine (CAPB) and remediation amendment calcium polysulfide (CPS) had no affect on microbial growth using BiologTM Ecoplates. To move towards a more realistic field analog, soil columns were packed with Hanford 200 Area sediment. Once microbial growth in the column was verified by observing growth of the effluent solution on tryptic soy agar plates, remedial surfactants were injected into the columns, and the resulting metabolic diversity was measured. Results suggest surfactant sodium dodecyl sulfate (SDS) stimulates microbial growth. The soil columns were also visualized using X-ray microtomography to inspect soil packing and possibly probe for evidence of biofilms. Overall, BiologTM Ecoplates provide a rapid assay to predict effects of remediation amendments on Hanford 200 area deep vadose zone microorganisms.

Miller, Hannah M.; Tilton, Fred A.

2012-08-10T23:59:59.000Z

98

Re-engineering bacteria for ethanol production  

DOE Patents [OSTI]

The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.

Yomano, Lorraine P; York, Sean W; Zhou, Shengde; Shanmugam, Keelnatham; Ingram, Lonnie O

2014-05-06T23:59:59.000Z

99

Organic acid-tolerant microorganisms and uses thereof for producing organic acids  

DOE Patents [OSTI]

Organic acid-tolerant microorganisms and methods of using same. The organic acid-tolerant microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid (3HP), acrylic acid, and propionic acid. Further modifications to the microorganisms such as increasing expression of malonyl-CoA reductase and/or acetyl-CoA carboxylase provide or increase the ability of the microorganisms to produce 3HP. Methods of generating an organic acid with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers include replacing acsA or homologs thereof in cells with genes of interest and selecting for the cells comprising the genes of interest with amounts of organic acids effective to inhibit growth of cells harboring acsA or the homologs.

Pfleger, Brian Frederick; Begemann, Matthew Brett

2014-05-06T23:59:59.000Z

100

E-Print Network 3.0 - airborne moisture-indicating microorganisms...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

surface. In order to investigate the microorganisms present... obtained on the Marine Agar plates indicating a possible marine source for some of these airborne Source: Rainey,...

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


101

E-Print Network 3.0 - acidophilic microorganisms isolated Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Thermo-acidophilic Cyanidiophyceae (Cyanidiales)" Summary: AND DEBASHISH BHATTACHARYA Johnson, D.B. (1998) Biodiversity and ecology of acidophilic microorganisms. FEMS... Biodata...

102

E-Print Network 3.0 - aerobic bio-oxicdation combined Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

(contain oxygen) for treatment to occur. According to current state law... (Rule 410 IAC 6-8.1), the soil aerobic zone below the soil absorption field must be at least 2...

103

E-Print Network 3.0 - aerobic methane release Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

by Explorit Topic List Advanced Search Sample search results for: aerobic methane release Page: << < 1 2 3 4 5 > >> 1 I.Vigano1, T.Rckmann1, R.Holzinger1, A. van Dijk2, F.Keppler3,...

104

Aerobic capacities in the heart, kidneys, and splanchnic organs of harbor seals: adaptations to diving hypoxia  

E-Print Network [OSTI]

Pinnipeds (seals and sea lions) have an elevated mitochondrial volume density [Vv(mt)] and citrate synthase (CS) and ?-hydroxyacyl-CoA dehydrogenase (HOAD) activity in their swimming muscles, to maintain aerobic metabolism, as an adaptation...

Fuson, Amanda Lynn

2002-01-01T23:59:59.000Z

105

Typical College Student Diet Food Derived Microorganisms and Their Relation to the Human Gastrointestinal Microflora  

E-Print Network [OSTI]

of the food samples were plated on Tryptic Soy Agar media and Brucella Blood Agar to determine the aerobic and anaerobic bacterial loads respectively. The total microbioial communities were extracted from defined amounts of the different food samples...

Haselhorst, Alexandria J.

2011-08-08T23:59:59.000Z

106

Sensory Transduction in Microorganisms 2008 Gordon Research Conference (January 2008)  

SciTech Connect (OSTI)

Research into the mechanisms involved in the sensing and responses of microorganisms to changes in their environments is currently very active in a large number of laboratories worldwide. An increasingly wide range of prokaryotic and eukaryotic species are being studied with regard to their sensing of diverse chemical and physical stimuli, including nutrients, toxins, intercellular signaling molecules, redox indicators, light, pressure, magnetic fields, and surface contact, leading to adaptive responses affecting motile behavior, gene expression and/or development. The ease of manipulation of microorganisms has facilitated application of a broad range of techniques that have provided comprehensive descriptions of cellular behavior and its underlying molecular mechanisms. Systems and their molecular components have been probed at levels ranging from the whole organism down to atomic resolution using behavioral analyses; electrophysiology; genetics; molecular biology; biochemical and biophysical characterization; structural biology; single molecule, fluorescence and cryo-electron microscopy; computational modeling; bioinformatics and genomic analyses. Several model systems such as bacterial chemotaxis and motility, fruiting body formation in Myxococcus xanthus, and motility and development in Dictyostelium discoideum have traditionally been a focus of this meeting. By providing a basis for assessment of similarities and differences in mechanisms, understanding of these pathways has advanced the study of many other microbial sensing systems. This conference aims to bring together researchers investigating different prokaryotic and eukaryotic microbial systems using diverse approaches to compare data, share methodologies and ideas, and seek to understand the fundamental principles underlying sensory responses. Topic areas include: (1) Receptor Sensing and Signaling; (2) Intracellular Signaling (two-component, c-di-GMP, c-AMP, etc.); (3) Intracellular Localization and the Cytoskeleton; (4) Motors and Motility; (5) Differentiation and Development; (6) Host/Pathogen and Host/Symbiont Interactions; (7) Intercellular Communication; (8) Microbes and the Environment; and (9) Modeling Signaling Pathways.

Ann M. Stock

2009-04-08T23:59:59.000Z

107

Transformation of gram positive bacteria by sonoporation  

SciTech Connect (OSTI)

The present invention provides a sonoporation-based method that can be universally applied for delivery of compounds into Gram positive bacteria. Gram positive bacteria which can be transformed by sonoporation include, for example, Bacillus, Streptococcus, Acetobacterium, and Clostridium. Compounds which can be delivered into Gram positive bacteria via sonoporation include nucleic acids (DNA or RNA), proteins, lipids, carbohydrates, viruses, small organic and inorganic molecules, and nano-particles.

Yang, Yunfeng; Li, Yongchao

2014-03-11T23:59:59.000Z

108

Managing Bacteria Pollution in Texas Waters  

E-Print Network [OSTI]

Story by Kathy Wythe tx H2O | pg. 2 BACTERIA MANAGING tx H2O | pg. 3 IN TEXAS WATERS POLLUTION Managing Bacteria Pollution in Texas Waters tx H2O | pg. 4 W ith 310 water bodies in Texas failing to meetwater quality standards because... of bacteria,managing bacteria pollution is commanding the attention of water agencies, researchers and stake- holders across Texas. These water bodies are listed in the 2006 Texas Water Quality Inventory and 303(d) List for failing to meet the standards...

Wythe, Kathy

2007-01-01T23:59:59.000Z

109

Development of Microorganisms with Improved Transport and Biosurfactant Activity for Enhanced Oil Recovery  

SciTech Connect (OSTI)

The project had three objectives: (1) to develop microbial strains with improved biosurfactant properties that use cost-effective nutrients, (2) to obtain biosurfactant strains with improved transport properties through sandstones, and (3) to determine the empirical relationship between surfactant concentration and interfacial tension and whether in situ reactions kinetics and biosurfactant concentration meets appropriate engineering design criteria. Here, we show that a lipopeptide biosurfactant produced by Bacillus mojavensis strain JF-2 mobilized substantial amounts of residual hydrocarbon from sand-packed columns and Berea sandstone cores when a viscosifying agent and a low molecular weight alcohol were present. The amount of residual hydrocarbon mobilized depended on the biosurfactant concentration. Tertiary oil recovery experiments showed that 10 to 40 mg/l of JF-2 biosurfactant in the presence of 0.1 mM 2,3-butanediol and 1 g/l of partially hydrolyzed polyacrylamide (PHPA) recovered 10-40% of residual oil from Berea sandstone cores. Even low biosurfactant concentrations (16 mg/l) mobilized substantial amounts of residual hydrocarbon (29%). The bio-surfactant lowered IFT by nearly 2 orders of magnitude compared to typical IFT values of 28-29 mN/m. Increasing the salinity increased the IFT with or without 2,3-butanediol present. The lowest interfacial tension observed was 0.1 mN/m. A mathematical model that relates oil recovery to biosurfactant concentration was modified to include the stepwise changes in IFT as biosurfactant concentrations changes. This model adequately predicted the experimentally observed changes in IFT as a function of biosurfactant concentration. Theses data show that lipopeptide biosurfactant systems may be effective in removing hydrocarbon contamination sources in soils and aquifers and for the recovery of entrapped oil from low production oil reservoirs. Diverse microorganisms were screened for biosurfactant production and anaerobic growth at elevated salt concentrations to obtain candidates most suitable for microbial oil recovery. Seventy percent of the 205 strains tested, mostly strains of Bacillus mojavensis, Bacillus subtilis, Bacillus licheniformis, and Bacillus sonorensis, produced biosurfactants aerobically and 41% of the strains had biosurfactant activity greater than Bacillus mojavensis JF-2, the current candidate for oil recovery. Biosurfactant activity varied with the percentage of the 3-hydroxy-tetradecanoate isomers in the fatty acid portion of the biosurfactant. Changing the medium composition by incorporation of different precursors of 3-hydroxy tetradecanoate increased the activity of biosurfactant. The surface tension and critical micelle concentration of 15 different, biosurfactant-producing Bacillus strains was determined individually and in combination with other biosurfactants. Some biosurfactant mixtures were found to have synergistic effect on surface tension (e.g. surface tension was lowered from 41 to 31 mN/m in some cases) while others had a synergistic effect on CMD-1 values. We compared the transport abilities of spores from three Bacillus strains using a model porous system to study spore recovery and transport. Sand-packed columns were used to select for spores or cells with the best transport abilities through brine-saturated sand. Spores of Bacillus mojavensis strains JF-2 and ROB-2 and a natural recombinant, strain C-9, transported through sand at very high efficiencies. The earliest cells/spores that emerged from the column were regrown, allowed to sporulate, and applied to a second column. This procedure greatly enhanced the transport of strain C-9. Spores with enhanced transport abilities can be easily obtained and that the preparation of inocula for use in MEOR is feasible. We conducted a push-pull test to study in-situ biosurfactant production by exogenous biosurfactant producers to aid in oil recovery from depleted reservoirs. Five wells from the same formation were used. Two wells received cells and nutrients, two wells were treated with nutrients onl

M.J. McInerney; K.E. Duncan; N. Youssef; T. Fincher; S.K. Maudgalya; M.J. Folmsbee; R. Knapp; Randy R. Simpson; N.Ravi; D. Nagle

2005-08-15T23:59:59.000Z

110

Final Technical Report: Viral Infection of Subsurface Microorganisms and Metal/Radionuclide Transport  

SciTech Connect (OSTI)

Microbially mediated metabolisms have been identified as a significant factor either directly or indirectly impacting the fate and transport of heavy metal/radionuclide contaminants. To date microorganisms have been isolated from contaminated environments. Examination of annotated finished genome sequences of many of these subsurface isolates from DOE sites, revealed evidence of prior viral infection. To date the role that viruses play influencing microbial mortality and the resulting community structure which directly influences biogeochemical cycling in soils and sedimentary environments remains poorly understood. The objective of this exploratory study was to investigate the role of viral infection of subsurface bacteria and the formation of contaminant-bearing viral particles. This objective was approached by examining the following working hypotheses: (i) subsurface microorganisms are susceptible to viral infections by the indigenous subsurface viral community, and (ii) viral surfaces will adsorb heavy metals and radionuclides. Our results have addressed basic research needed to accomplish the BER Long Term Measure to provide sufficient scientific understanding such that DOE sites would be able to incorporate coupled physical, chemical and biological processes into decision making for environmental remediation or natural attenuation and long-term stewardship by establishing viral-microbial relationships on the subsequent fate and transport of heavy metals and radionuclides. Here we demonstrated that viruses play a significant role in microbial mortality and community structure in terrestrial subsurface sedimentary systems. The production of viral-like particles within subsurface sediments in response to biostimulation with dissolved organic carbon and a terminal electron acceptor resulted in the production of viral-like particles. Organic carbon alone did not result in significant viral production and required the addition of a terminal electron acceptor (nitrate), indicating that nutrients are not limiting viral production, but rather substrates that can be converted into energy for host metabolism. Our results also revealed that cell abundance was not correlated to the mineralization of organic carbon, but rather viruses were positively correlated with carbon mineralization. This is a result of viral-mediated cell lysis and demonstrates that viruses are sensitive indicators of microbial activity. Viruses as an indicator of microbial activity was not unique to batch culture studies as results obtained from an in situ field experiment conducted at the DOE Old Rifle Field site. This study revealed that viral abundance increased in response to the injection of oxygenated groundwater and influx of dissolved organic carbon whereas cell abundance changes were minimal. However, the extent to which viral-mediated cell lysis alters organic matter pools subsequently influencing microbial community structure and biogeochemical function remains a critical question in subsurface biogeochemical cycling. The production of significant numbers of viruses in groundwater has implications for nanoparticulate metal as well as carbon transport in groundwater. We have demonstrated that the virus surface is reactive and will adsorb heavy metals. Thus viruses can promote colloidal contaminant mobility. Interestingly, the presence of heavy metals has a positive effect on infectivity of the phage, increasing phage infection which could lead to further production of viruses. Together, the results indicate that the sorption of metals to the surface of viruses could not only contribute to nanoparticulate metal as well as carbon transport but could also enhance infectivity further contributing to cell lysis which could subsequently influence biogeochemical cycling. As more viruses infect host microbial populations the high concentration of metals would enhance infection, resulting in cell lysis, and decreasing the metabolically active host population while yielding greater numbers of viruses capable of transporting contaminats. Additional studie

Weber, Karrie A.; Bender, Kelly S.; Li, Yusong

2013-09-28T23:59:59.000Z

111

Aerobic treatability of waste effluent from the leather finishing industry. Master's thesis  

SciTech Connect (OSTI)

The Seton Company supplies finished leather products exclusively for the automotive industry. In the process of finishing leather, two types of wastewaters are generated. The majority of the wastewater is composed of water-based paint residuals while the remainder is composed of solvent-based coating residuals. Aerobic treatability studies were conducted using water-based and solvent-based waste recirculatory waters from the Seton Company's Saxton, Pennsylvania processing plant. The specific objective was to determine the potential for using aerobic biological processes to biodegrade the industry's wastes and determine the potential for joint treatment at the local publicly owned treatment works (POTW). This study was accomplished in two phases. Phase I was conducted during the Spring Semester 1993 and consisted of aerobic respirometer tests of the raw wastes and mass balance analysis. The results of Phase I were published in a report to the Seton Company as Environmental Resources Research Institute project number 92C.II40R-1. Phase II was conducted during the Summer Semester 1993 and consisted of bench-scale reactor tests and additional aerobic respirometer tests. The aerobic respirometer batch tests and bench-scale reactor tests were used to assess the treatability of solvent-based and water-based wastewaters and determine the degree of biodegradability of the wastewaters. Mass balance calculations were made using measured characteristics.

Vinger, J.A.

1993-12-01T23:59:59.000Z

112

Aggregation Patterns in Stressed Bacteria  

SciTech Connect (OSTI)

We study the formation of spot patterns seen in bacterial colonies when the bacteria are subjected to oxidative stress due to hazardous by-products of respiration. The cell density is coupled to a chemoattractant concentration as well as to nutrient and waste fields. The model combines the propagation of a front of motile bacterial radially outward from an initial site, a Turing instability of the uniformly dense state, and a reduction of motility for cells sufficiently far behind the front. The wide variety of patterns seen in the experiments is reproduced by the model by varying the details of the initiation of the chemoattractant emission as well as the transition to a nonmotile phase.

Tsimring, L.; Levine, H. [Institute for Nonlinear Science, University of California at San Diego, La Jolla, California 92093-0402 (United States)] [Institute for Nonlinear Science, University of California at San Diego, La Jolla, California 92093-0402 (United States); Aranson, I. [Deptartment of Physics, Bar-Ilan University, Ramat Gan (Israel)] [Deptartment of Physics, Bar-Ilan University, Ramat Gan (Israel); Ben-Jacob, E.; Cohen, I.; Shochet, O. [School of Physics and Astronomy, Raymond & Beverly Sackler Faculty of Exact Sciences, Tel-Aviv University, Tel-Aviv 69978 (Israel)] [School of Physics and Astronomy, Raymond & Beverly Sackler Faculty of Exact Sciences, Tel-Aviv University, Tel-Aviv 69978 (Israel); Reynolds, W.N. [Complex Systems Division, Los Alamos National Laboratory, Los Alamos New Mexico (United States)] [Complex Systems Division, Los Alamos National Laboratory, Los Alamos New Mexico (United States)

1995-08-28T23:59:59.000Z

113

Identification of subsurface microorganisms at Yucca Mountain; Fourth quarterly report  

SciTech Connect (OSTI)

Bacteria isolated from water samples collected in a series of ground water springs have been isolated, enumerated, and identified from twenty six sites. Ten sites were sampled in Death Valley, California and sixteen sites were sampled in Ash Meadows, Nevada. Replicate samples were collected and tested from four locations. All water samples were collected in conjunction with the HRC chemistry group conducting ground water fingerprinting studies. The protocol for collection of samples, as described in the 3rd quarterly report, specified aseptic collection in sterile screw-capped containers and transportation on ice to the HRC microbiology laboratory. All samples were inoculated by spread plating onto R2A (Difco Laboratories, Detroit, MI) bacterial culture medium. the R2A plates were then incubated at 28{degrees} for 5--7 days and colonies wee counted with the aid of a grid template and magnifying lens.

Stetzenbach, L.D.

1993-08-01T23:59:59.000Z

114

Method of dispersing a hydrocarbon using bacteria  

DOE Patents [OSTI]

New protozoan derived microbial consortia and method for their isolation are provided. Consortia and bacteria isolated therefrom are useful for treating wastes such as trichloroethylene and trinitrotoluene. Consortia, bacteria isolated therefrom, and dispersants isolated therefrom are useful for dispersing hydrocarbons such as oil, creosote, wax, and grease.

Tyndall, Richard L. (Clinton, TN)

1996-01-01T23:59:59.000Z

115

Removal of polychlorinated phenols in sequential anaerobic-aerobic biofilm reactors packed with tire chips  

SciTech Connect (OSTI)

Scrap vehicle tire chips were used as packing material for sequential anaerobic-aerobic biofilm reactors to remove persistent chlorinated hydrocarbons. Adsorption capacity of scrap tires was greater under acidic conditions than under basic conditions. However, it was only approximately 0.04 to 0.3% of that of activated carbon. The amount of biomass that attached to the surface of scrap tires was 3.16 and 3.72 mg volatile suspended solids/cm{sup 2} after 14 and 37 days, respectively. Two laboratory-scale, down-flow anaerobic-aerobic biofilm reactors packed with tire chips were operated to remove 2,4-dichlorophenol (DCP) and 4-chlorophenol (CP). More than 98% of DCP was dehalogenated to CP in the anaerobic reactor, 70 to 98% of which was subsequently degraded in the aerobic reactor. Scrap tires did not cause any operational problems when used as biofilter media.

Shin, H.S.; Yoo, K.S.; Park, J.K.

1999-05-01T23:59:59.000Z

116

HYDROCARBON-DEGRADING BACTERIA AND SURFACTANT ACTIVITY  

SciTech Connect (OSTI)

Fate of benzene ethylbenzene toluene xylenes (BTEX) compounds through biodegradation was investigated using two different bacteria, Ralstonia picketti (BP-20) and Alcaligenes piechaudii (CZOR L-1B). These bacteria were isolated from extremely polluted petroleum hydrocarbon contaminated soils. PCR and Fatty Acid Methyl Ester (FAME) were used to identify the isolates. Biodegradation was measured using each organism individually and in combination. Both bacteria were shown to degrade each of the BTEX compounds. Alcaligenes piechaudii biodegraded BTEXs more efficiently while mixed with BP-20 and individually. Biosurfactant production was observed by culture techniques. In addition 3-hydroxy fatty acids, important in biosurfactant production, was observed by FAME analysis. In the all experiments toluene and m+p- xylenes were better growth substrates for both bacteria than the other BTEX compounds. In addition, the test results indicate that the bacteria could contribute to bioremediation of aromatic hydrocarbons (BTEX) pollution increase biodegradation through the action by biosurfactants.

Brigmon, R; Topher Berry, T; Grazyna A. Plaza, G; jacek Wypych, j

2006-08-15T23:59:59.000Z

117

Chemotactic selection of pollutant degrading soil bacteria  

DOE Patents [OSTI]

A method is described for identifying soil microbial strains which may be bacterial degraders of pollutants. This method includes: Placing a concentration of a pollutant in a substantially closed container; placing the container in a sample of soil for a period of time ranging from one minute to several hours; retrieving the container and collecting its contents; microscopically determining the identity of the bacteria present. Different concentrations of the pollutant can be used to determine which bacteria respond to each concentration. The method can be used for characterizing a polluted site or for looking for naturally occurring biological degraders of the pollutant. Then bacteria identified as degraders of the pollutant and as chemotactically attracted to the pollutant are used to innoculate contaminated soil. To enhance the effect of the bacteria on the pollutant, nutrients are cyclicly provided to the bacteria then withheld to alternately build up the size of the bacterial colony or community and then allow it to degrade the pollutant.

Hazen, T.C.

1991-03-04T23:59:59.000Z

118

Structural model and spectroscopic characteristics of the FMO antenna protein from the aerobic chlorophototroph, Candidatus Chloracidobacterium thermophilum  

SciTech Connect (OSTI)

The Fenna–Matthews–Olson protein (FMO) binds seven or eight bacteriochlorophyll a (BChl a) molecules and is an important model antenna system for understanding pigment-protein interactions and mechanistic aspects of photosynthetic light harvesting. FMO proteins of green sulfur bacteria (Chlorobiales) have been extensively studied using a wide range of spectroscopic and theoretical approaches because of their stability, the spectral resolution of their pigments, their water-soluble nature, and the availability of high-resolution structural data. We obtained new structural and spectroscopic insights by studying the FMO protein from the recently discovered, aerobic phototrophic acidobacterium, Candidatus Chloracidobacterium thermophilum. Native C. thermophilum FMO is a trimer according to both analytical gel filtration and native-electrospray mass spectrometry. Furthermore, the mass of intact FMO trimer is consistent with the presence of 21–24 BChl a in each. Homology modeling of the C. thermophilum FMO was performed by using the structure of the FMO protein from Chlorobaculum tepidum as a template. C. thermophilum FMO differs from C. tepidum FMO in two distinct regions: the baseplate, CsmA-binding region and a region that is proposed to bind the reaction center subunit, PscA. C. thermophilum FMO has two fluorescence emission peaks at room temperature but only one at 77 K. Temperature-dependent fluorescence spectroscopy showed that the two room-temperature emission peaks result from two excited-state BChl a populations that have identical fluorescence lifetimes. Modeling of the data suggests that the two populations contain 1–2 BChl and 5–6 BChl a molecules and that thermal equilibrium effects modulate the relative population of the two emitting states.

Wen, Jianzhong; Tsukatani, Yusuke; Cui, Weidong; Zhang, Hao; Gross, Michael L; Bryant, Donald A; Blankenship, R. E.

2011-01-01T23:59:59.000Z

119

In mammals, aerobic exercise is fuelled mainly by carbohydrates and lipids (Weber, 1992). In the second paper  

E-Print Network [OSTI]

In mammals, aerobic exercise is fuelled mainly by carbohydrates and lipids (Weber, 1992 and mitochondrial lipid oxidation are both scaled with aerobic capacity (Weber et al. 1993; McClelland et al. 1994 JEAN-MICHEL WEBER1,2,*, GÃ?RARD BRICHON1,, GEORGES ZWINGELSTEIN1,, GRANT MCCLELLAND1,2, CHRISTOPHER

McClelland, Grant B.

120

Perchlorate Reduction by Autotrophic Bacteria in the  

E-Print Network [OSTI]

-limiting step with complete conversion to chloride (7). Perchlorate reducing microorganisms (PRMs) are ubiq as the result of growth of heterotrophic microbes in the aquifer, not just PRMs (9). Thus, an important factor). As an alternative, H2 gas has significant advantages as an electron donor; it minimizes biomass clogging and can

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


121

Bioremediation of the organophosphate pesticide, coumaphos, using microorganisms immobilized in calcium-alginate gel beads  

E-Print Network [OSTI]

and diethylthiophosphate (DETP), using Ca-alginate immobilized cells was the focus of this research. Consortia of indigenous microorganisms capable of degrading chlorferon and DETP were isolated separately. Since chlorferon inhibited both chlorferon-degrading and DETP-degrading...

Ha, Jiyeon

2007-04-25T23:59:59.000Z

122

Technical Note: Evaluation of Effective Microorganisms (EM) In Solid Waste Management  

E-Print Network [OSTI]

Microorganisms (EM) In Solid Waste Management V. Sekeran C.is the treatment, disposal and/or recycling of solid wastes.Generally solid waste from a municipality consists of

Sekeran, V.; Balaji, C.; Bhagavathipushpa, T.

2005-01-01T23:59:59.000Z

123

E-Print Network 3.0 - anaerobic sediment microorganisms Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

microorganisms are also capable of transferring electrons to other metals and metalloids. Micro- bial reduction... of Fe(III) and other metals can influence the fate of metals in...

124

Nucleic acid molecules conferring enhanced ethanol tolerance and microorganisms having enhanced tolerance to ethanol  

DOE Patents [OSTI]

The present invention provides isolated nucleic acid molecules which encode a mutant acetaldehyde-CoA/alcohol dehydrogenase or mutant alcohol dehydrogenase and confer enhanced tolerance to ethanol. The invention also provides related expression vectors, genetically engineered microorganisms having enhanced tolerance to ethanol, as well as methods of making and using such genetically modified microorganisms for production of biofuels based on fermentation of biomass materials.

Brown, Steven; Guss, Adam; Yang, Shihui; Karpinets, Tatiana; Lynd, Lee; Shao, Xiongjun

2014-01-14T23:59:59.000Z

125

Anaerobic Redox Cycling of Iron by Freshwater Sediment Microorganisms  

SciTech Connect (OSTI)

The potential for microbially-mediated anaerobic redox cycling of iron (Fe) was examined in a first-generation enrichment culture of freshwater wetland sediment microorganisms. MPN enumerations revealed the presence of significant populations of Fe(III)-reducing (ca. 108 cells mL-1) and Fe(II)-oxidizing, nitrate-reducing organisms (ca. 105 cells mL-1) in the sediment used to inoculate the enrichment cultures. Nitrate reduction commenced immediately following inoculation of acetate-containing (ca. 1 mM) medium with a small quantity (1% vol/vol) of wetland sediment, and resulted in the transient accumulation of NO2- and production of a mixture of end-products including NH4+. Fe(III) oxide (high surface area goethite) reduction took place - after NO3- was depleted and continued until all the acetate was utilized. Addition of NO3 after Fe(III) reduction ceased resulted in the immediate oxidation of Fe(II) coupled to reduction of + NO3-to NH4 . No significant NO2- accumulation was observed during nitrate-dependent Fe(II) oxidation. No Fe(II) oxidation occurred in pasteurized controls. Microbial community structure in the enrichment was monitored by DGGE analysis of PCR amplified 16s rDNA and RT-PCR amplified 16S rRNA, as well as by construction of 16S rDNA clone libraries for four different time points during the experiment. Strong similarities in dominant members of the microbial community were observed in the Fe(III) reduction and nitrate-dependent Fe(II) oxidation phases of the experiment, specifically the common presence of organisms closely related (= 95% sequence similarity) to the genera Geobacter and Dechloromonas. These results indicate that the wetland sediments contained organisms such as Geobacter sp. which are capable of both + dissimilatory Fe(III) reduction and oxidation of Fe(II) with reduction of NO3-reduction to NH4 . Our findings suggest that microbially-catalyzed nitrate-dependent Fe(II) oxidation has the potential to contribute to a dynamic anaerobic Fe redox cycle in freshwater sediments.

Weber, Karrie A.; Urrutia, Matilde M.; Churchill, Perry F.; Kukkadapu, Ravi K.; Roden, Eric E.

2006-01-01T23:59:59.000Z

126

Recovery of uranium by using new microorganisms isolated from North American uranium deposits  

SciTech Connect (OSTI)

Some attempts were made to remove uranium that may be present in refining effluents, mine tailings by using new microorganisms isolated from uranium deposits and peculiar natural environments. To screen microorganisms isolated from uranium deposits and peculiar natural environments in North America and Japan for maximal accumulation of uranium, hundreds of microorganisms were examined. Some microorganisms can accumulate about 500 mg (4.2 mEq) of uranium per gram of Microbial cells within 1 h. The uranium accumulation capacity of the cells exceeds that of commercially available chelating agents (2-3 mEq/g adsorbent). We attempted to recover uranium from uranium refining waste water by using new microorganisms. As a result, these microbial cells can recover trace amounts of uranium from uranium waste water with high efficiency. These strains also have a high accumulating ability for thorium. Thus, these new microorganisms can be used as an adsorbing agent for the removal of nuclear elements may be present in metallurgical effluents, mine tailings and other waste sources.

Sakaguchi, T.; Nakajima, A.; Tsuruta, T. [Miyazaki Medical College (Japan)

1995-12-31T23:59:59.000Z

127

Assessing the Efficacy of the Aerobic Methanotrophic Biofilter in Methane Hydrate Environments  

SciTech Connect (OSTI)

In October 2008 the University of California at Santa Barbara (UCSB) initiated investigations of water column methane oxidation in methane hydrate environments, through a project funded by the National Energy Technology Laboratory (NETL) entitled: assessing the efficacy of the aerobic methanotrophic biofilter in methane hydrate environments. This Final Report describes the scientific advances and discoveries made under this award as well as the importance of these discoveries in the broader context of the research area. Benthic microbial mats inhabit the sea floor in areas where reduced chemicals such as sulfide reach the more oxidizing water that overlies the sediment. We set out to investigate the role that methanotrophs play in such mats at locations where methane reaches the sea floor along with sulfide. Mats were sampled from several seep environments and multiple sets were grown in-situ at a hydrocarbon seep in the Santa Barbara Basin. Mats grown in-situ were returned to the laboratory and used to perform stable isotope probing experiments in which they were treated with 13C-enriched methane. The microbial community was analyzed, demonstrating that three or more microbial groups became enriched in methane?s carbon: methanotrophs that presumably utilize methane directly, methylotrophs that presumably consume methanol excreted by the methanotrophs, and sulfide oxidizers that presumably consume carbon dioxide released by the methanotrophs and methylotrophs. Methanotrophs reached high relative abundance in mats grown on methane, but other bacterial processes include sulfide oxidation appeared to dominate mats, indicating that methanotrophy is not a dominant process in sustaining these benthic mats, but rather a secondary function modulated by methane availability. Methane that escapes the sediment in the deep ocean typically dissolved into the overlying water where it is available to methanotrophic bacteria. We set out to better understand the efficacy of this process as a biofilter by studying the distribution of methane oxidation and disposition of methanotrophic populations in the Pacific Ocean. We investigated several environments including the basins offshore California, the continental margin off Central America, and the shallow waters around gas seeps. We succeeded in identifying the distributions of activity in these environments, identified potential physical and chemical controls on methanotrophic activity, we further revealed details about the methanotrophic communities active in these settings, and we developed new approaches to study methanotrophic communities. These findings should improve our capacity to predict the methanotrophic response in ocean waters, and further our ability to generate specific hypotheses as to the ecology and efficacy of pelagic methanotrophic communites. The discharge of methane and other hydrocarbons to Gulf of Mexico that followed the sinking of the Deepwater Horizon provided a unique opportunity to study the methanotorphic biofilter in the deep ocean environment. We set out to understand the consumption of methane and the bloom of methanotrophs resulting from this event, as a window into the regional scale release of gas hydrate under rapid warming scenarios. We found that other hydrocarbon gases, notably propane and ethane, were preferred for consumption over methane, but that methane consumption accelerated rapidly and drove the depletion of methane within a matter of months after initial release. These results revealed the identity of the responsible community, and point to the importance of the seed population in determining the rate at which a methanotrophic community is able to respond to an input of methane. Collectively, these results provide a significant advance in our understanding of the marine methanotrohic biofilter, and further provide direction and context for future investigations of this important phenomenon. This project has resulted in fourteen publications to date, with five more circulating in draft form, and several others planned.

Valentine, David

2012-09-30T23:59:59.000Z

128

REVIEW ARTICLE Aerobic rice for water-saving agriculture. A review  

E-Print Network [OSTI]

experience "economic water scarcity" by 2025 (Tuong and Bouman 2003). The increasing shortage of waterREVIEW ARTICLE Aerobic rice for water-saving agriculture. A review Lixiao Nie & Shaobing Peng /Published online: 7 October 2011 # INRA ­ DIB / Springer-Verlag France 2011 Abstract The increasing shortage

Boyer, Edmond

129

The Effect of Slowly Biodegradable Carbon on the Morphology, Integrity and Performance of Aerobic Granular Sludge  

E-Print Network [OSTI]

and phosphorus. For both experimental phases, the hydraulic loading rate was 1.32 kg COD/m3/day. For the 1st phase, the three reactors were fed soluble organic matter (sodium acetate) as the sole carbon source with an anaerobic-aerobic SBR cycle. To achieve...

Faraj, Rasha Attwan

2014-08-31T23:59:59.000Z

130

Water Research 36 (2002) 37393746 Effect of ethanol on BTEX biodegradation kinetics: aerobic  

E-Print Network [OSTI]

Water Research 36 (2002) 3739­3746 Effect of ethanol on BTEX biodegradation kinetics: aerobic November 2001; received in revised form 1 February 2002 Abstract The use of ethanol as an automotive fuel oxygenate represents potential economic and air-quality benefits. However, little is known about how ethanol

Alvarez, Pedro J.

131

Biodegradation of triclosan and formation of methyl-triclosan in activated sludge under aerobic conditions  

E-Print Network [OSTI]

Biodegradation of triclosan and formation of methyl-triclosan in activated sludge under aerobic in activated sludge from a standard activated sludge WWTP equipped with enhanced biological phosphorus removal) and anaerobic conditions rather low removal rates were determined. In a laboratory-scale activated sludge

Kolaei, Alireza Rezania

132

Aerobic Capacity, Activity Levels and Daily Energy Expenditure in Male and Female Adolescents of the  

E-Print Network [OSTI]

. The highly active and energy-demanding lifestyle of rural Kenyan adolescents may accountAerobic Capacity, Activity Levels and Daily Energy Expenditure in Male and Female Adolescents travelled to school and daily energy expenditure in 15 habitually active male (13.961.6 years) and 15

Lieberman, Daniel E.

133

aerobic energy metabolism1woa: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

aerobic energy metabolism1woa First Page Previous Page 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 Misconceptions about...

134

Determination of kinetic coefficients for the simultaneous reduction of sulfate and uranium by Desulfovibrio desulfuricans bacteria  

SciTech Connect (OSTI)

Uranium contamination of groundwaters and surface waters near abandoned mill tailings piles is a serious concern in many areas of the western United States. Uranium usually exists in either the U(IV) or the U(VI) oxidation state. U(VI) is soluble in water and, as a result, is very mobile in the environment. U(IV), however, is generally insoluble in water and, therefore, is not subject to aqueous transport. In recent years, researchers have discovered that certain anaerobic microorganisms, such as the sulfate-reducing bacteria Desulfovibrio desulfuricans, can mediate the reduction of U(VI) to U(IV). Although the ability of this microorganism to reduce U(VI) has been studied in some detail by previous researchers, the kinetics of the reactions have not been characterized. The purpose of this research was to perform kinetic studies on Desulfovibrio desulficans bacteria during simultaneous reduction of sulfate and uranium and to determine the phase in which uranium exists after it has been reduced and precipitated from solution. The studies were conducted in a laboratory-scale chemostat under substrate-limited growth conditions with pyruvate as the substrate. Kinetic coefficients for substrate utilization and cell growth were calculated using the Monod equation. The maximum rate of substrate utilization (k) was determined to be 4.70 days{sup {minus}1} while the half-velocity constant (K{sub s}) was 140 mg/l COD. The yield coefficient (Y) was determined to be 0.17 mg cells/mg COD while the endogenous decay coefficient (k{sub d}) was calculated as 0.072 days{sup {minus}1}. After reduction, U(IV) Precipitated from solution in the uraninite (UO{sub 2}) phase. Uranium removal efficiency as high as 90% was achieved in the chemostat.

Tucker, M.D.

1995-05-01T23:59:59.000Z

135

Reverse and flick: Hybrid locomotion in bacteria  

E-Print Network [OSTI]

Many bacteria are motile. They use one or more helical flagella as propellers, rotating them like the corkscrew on a wine bottle opener. Despite the limited morphological repertoire of the propulsive system, radically ...

Stocker, Roman

136

Probiotic Bacteria Induce a ‘Glow of Health’  

E-Print Network [OSTI]

Radiant skin and hair are universally recognized as indications of good health. However, this ‘glow of health’ display remains poorly understood. We found that feeding of probiotic bacteria to aged mice induced integumentary ...

Levkovich, Tatiana

137

Fuel from Bacteria: Bioconversion of Carbon Dioxide to Biofuels by Facultatively Autotrophic Hydrogen Bacteria  

SciTech Connect (OSTI)

Electrofuels Project: Ohio State is genetically modifying bacteria to efficiently convert carbon dioxide directly into butanol, an alcohol that can be used directly as a fuel blend or converted to a hydrocarbon, which closely resembles a gasoline. Bacteria are typically capable of producing a certain amount of butanol before it becomes too toxic for the bacteria to survive. Ohio State is engineering a new strain of the bacteria that could produce up to 50% more butanol before it becomes too toxic for the bacteria to survive. Finding a way to produce more butanol more efficiently would significantly cut down on biofuel production costs and help make butanol cost competitive with gasoline. Ohio State is also engineering large tanks, or bioreactors, to grow the biofuel-producing bacteria in, and they are developing ways to efficiently recover biofuel from the tanks.

None

2010-07-01T23:59:59.000Z

138

Agencies Approve Bacteria TMDL Task Force Recommendations  

E-Print Network [OSTI]

their agencies to update their TMDL guidance documents to reflect these recommendations. They also authorized establishing a multi-agency bacteria TMDL work group to examine the research and development needs identified in the task force report. Both TCEQ... activities needed to strengthen the scientific tools available for TMDL and I-Plan development. The report and related documents are available at twri.tamu.edu/bacteriatmdl/. Tier 1 Analysis (T1) (one-year) Required for all bacteria TMDLs. ? Form TMDL...

Wythe, Kathy

2007-01-01T23:59:59.000Z

139

Phase Preference by Active, Acetate-Utilizing Bacteria at the Rifle, CO Integrated Field Research Challenge Site  

SciTech Connect (OSTI)

Uranium contaminated groundwaters are a legacy concern for the U.S. Department of Energy. Previous experiments at the Rifle, Colorado Integrated Field Challenge (IFC) site have demonstrated that field-scale addition of acetate to groundwater reduces the ambient soluable uranium concentration, sequestering the radionuclide as uraninite. However, questions remain regarding which microorganism(s) are consuming this acetate and if active groundwater microorganisms are different from active particle-associated bacteria. In this report, 13-C acetate was used to assess the active microbes that synthesize DNA on 3 size fractions [coarse sand, fines (8-approximately 150 micron), groundwater (0.2-8 micron)] over a 24 -day time frame. Results indicated a stronger signal from 13-C acetate associated with the “fines” fraction compared with smaller amounts of 13-C uptake on the sand fraction and groundwater samples during the SIP incubations. TRFLP analysis of this 13-C-labeled DNA, indicated 31+ 9 OTU's with 6 peaks dominating the active profiles (166, 187, 210, 212, and 277 bp peaks using MnlI). Cloning/sequencing of the amplification products indicated a Geobacter-like group (187, 210, 212 bp) primarily synthesized DNA from acetate in the groundwater phase, an alpha Proteobacterium (166 bp) primarily grew on the fines/sands, and an Acinetobacter sp. (277 bp) utilized much of the 13C acetate in both groundwater and particle-associated phases. These findings will help to delineate the acetate utilization patterns of bacteria during field-scale acetate addition and can lead to improved methods for stimulating distinct microbial populations in situ.

Kerkhoff, Lee; Williams, Kenneth H.; Long, Philip E.; McGuinness, L.

2011-02-15T23:59:59.000Z

140

E-Print Network 3.0 - aerobic wastewater biofilms Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Membrane... biofilm reactor Introduction One of the major challenges in wastewater treatment is achieving effective... et al. 2004). Nitrifying bacteria grow in the deep,...

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


141

E-Print Network 3.0 - aerobic alcohol oxidation Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

enzymes in Gram-positive methylotrophic bacteria Summary: in vivo role is in oxidation of methanol and other primary alcohols. B. methanolicus displays activity... -producing and...

142

Chemotactic selection of pollutant degrading soil bacteria  

DOE Patents [OSTI]

A method for identifying soil microbial strains which may be bacterial degraders of pollutants comprising the steps of placing a concentration of a pollutant in a substantially closed container, placing the container in a sample of soil for a period of time ranging from one minute to several hours, retrieving the container, collecting the contents of the container, and microscopically determining the identity of the bacteria present. Different concentrations of the pollutant can be used to determine which bacteria respond to each concentration. The method can be used for characterizing a polluted site or for looking for naturally occurring biological degraders of the pollutant. Then bacteria identified as degraders of the pollutant and as chemotactically attracted to the pollutant are used to inoculate contaminated soil. To enhance the effect of the bacteria on the pollutant, nutrients are cyclicly provided to the bacteria then withheld to alternately build up the size of the bacterial colony or community and then allow it to degrade the pollutant.

Hazen, Terry C. (Augusta, GA)

1994-01-01T23:59:59.000Z

143

Wastewater treatment by aerobic granular biofilmWastewater treatment by aerobic granular biofilmaste ate t eat e t by ae ob c g a u a b o Aeration pulses to improve N eliminationAeration pulses to improve N-eliminationAeration pulses to improve N eliminat  

E-Print Network [OSTI]

Wastewater treatment by aerobic granular biofilmWastewater treatment by aerobic granular wastewater treatment p p denitrification Nitrification is the oxidation from ammonium (NH +) first activated sludge for biological N-elimination is a two step process: aerobic nitrification and anoxicp g g g

144

RexDalton,SanFrancisco Plans to use marine microorganisms as a  

E-Print Network [OSTI]

weaken the argument that oceanic carbon sequestration can act as a significant component in controlling weather in a region chosen for its significant impact on natural sequestration of carbon dioxide fromRexDalton,SanFrancisco Plans to use marine microorganisms as a sink for atmospheric carbon dioxide

Cai, Long

145

(SEMI-) AUTOMATIC RECOGNITION OF MICROORGANISMS IN WATER Karsten Rodenacker, Peter Gais1  

E-Print Network [OSTI]

of biocenosis in natural waters or investiga- tions on biocenosis in artificial, selective affected waters(SEMI-) AUTOMATIC RECOGNITION OF MICROORGANISMS IN WATER Karsten Rodenacker, Peter Gais1 , Uta J The structure of biocenosis is a powerful indicator for the condition of and changes in quality of the ecosystem

Rodenacker, Karsten

146

BIODEGRADATION OF MACONDO OIL BY AEROBIC HYDROCARBON?DEGRADING BACTERIA IN THE WATER COLUMN AND DEEPSEA SEDIMENTS OF THE NORTHERN GULF OF MEXICO.  

E-Print Network [OSTI]

??Previous studies have come to contrasting conclusions regarding nutrient limitation of hydrocarbon biodegradation in the Gulf of Mexico, and rate measurements are needed to support… (more)

Sun, Xiaoxu

2014-01-01T23:59:59.000Z

147

Human Occupancy as a Source of Indoor Airborne Bacteria  

E-Print Network [OSTI]

Exposure to specific airborne bacteria indoors is linked to infectious and noninfectious adverse health outcomes. However, the sources and origins of bacteria suspended in indoor air are not well understood. This study ...

Hospodsky, Denina

148

Hypermutation and adaptation of experimentally evolved marine Vibrio bacteria  

E-Print Network [OSTI]

Environmental bacteria display tremendous genetic diversity, but we are still learning how this diversity arises and relates to their wide range of habitats. Investigating how bacteria adapt helps us understand their ...

Clarke, Sean Aidan

2013-01-01T23:59:59.000Z

149

Raman activity in synchronously dividing bacteria  

SciTech Connect (OSTI)

Using a spectrometer equipped with an optical-multichannel analyzer as the detector (OMA), we have observed the Stokes laser-Raman spectra of metabolically active Escherichia coli and Bacillus megaterium from 100 - 2100 cm/sup -1/. After lengthy investigation, no Raman lines attributable to the metabolic process nor the cells themselves were found. Previous Raman spectra of active bacteria cannot be used to support nonlinear theories in biology. 34 refs., 9 figs.

Layne, S.P.

1985-01-01T23:59:59.000Z

150

Natural Oil Production from Microorganisms: Bioprocess and Microbe Engineering for Total Carbon Utilization in Biofuel Production  

SciTech Connect (OSTI)

Electrofuels Project: MIT is using carbon dioxide (CO2) and hydrogen generated from electricity to produce natural oils that can be upgraded to hydrocarbon fuels. MIT has designed a 2-stage biofuel production system. In the first stage, hydrogen and CO2 are fed to a microorganism capable of converting these feedstocks to a 2-carbon compound called acetate. In the second stage, acetate is delivered to a different microorganism that can use the acetate to grow and produce oil. The oil can be removed from the reactor tank and chemically converted to various hydrocarbons. The electricity for the process could be supplied from novel means currently in development, or more proven methods such as the combustion of municipal waste, which would also generate the required CO2 and enhance the overall efficiency of MIT’s biofuel-production system.

None

2010-07-15T23:59:59.000Z

151

Biochemistry and physiology of anaerobic bacteria  

SciTech Connect (OSTI)

We welcome you to The Power of Anaerobes. This conference serves two purposes. One is to celebrate the life of Harry D. Peck, Jr.,who was born May 18, 1927 and would have celebrated his 73rd birthday at this conference. He died November 20, 1998. The second is to gather investigators to exchange views within the realm of anaerobic microbiology, an area in which tremendous progress has been seen during recent years. It is sufficient to mention discoveries of a new form of life (the archaea), hyper or extreme thermophiles, thermophilic alkaliphiles and anaerobic fungi. With these discoveries has come a new realization about physiological and metabolic properties of microorganisms, and this in turn has demonstrated their importance for the development, maintenance and sustenance of life on Earth.

NONE

2000-05-18T23:59:59.000Z

152

3rd ASM Conference on Cell-Cell Communication in Bacteria  

SciTech Connect (OSTI)

This report summarizes the final program and provides the abstracts presented at the fourth American Society of Microbiology-sponsored conference on Cell-cell Communication in Bacteria, held November 6-9, 2011 in Miami, Florida. Bacteria are the paradigm for unicellular life, yet they also exhibit elaborate coordinated behaviors that often defy unicellularity. Research over the past two decades has revealed that a wide range of microbes communicate by diverse mechanisms. In most cases these microbial conversations occur through the exchange of diffusible signals, although there are also clear examples of contact-dependent communication. Many microbes use these signaling mechanisms to monitor and respond to population density, a process often described as quorum sensing. Interbacterial communication is not, however restricted to quorum sensing mechanisms, and there is mounting evidence that signaling can function in a range of different capacities. Communication between microorganisms has profound impacts on host interactions, as pathogens and commensals often regulate factors critical for interaction with their hosts via signal production and perception. The CCCB-4 conference provided a unique forum for the discussion, dissemination and exchange of new information and ideas among researchers working within this rapidly developing, yet mature field. Sessions were arranged around topics such as: the diversity of signal generation and identity; mechanisms of signal transduction and interference; cell-cell communication in bacterial development and antibiotic synthesis; host-microbe signaling and pathogenesis; symbiosis, mutualism, and microbe-microbe communication; ecology and evolution; advancements in the technological tool-kit for studying cell-cell communication. The conference served as a conduit for the exchange and synthesis of new ideas among leading US and international scientists working on bacterial communication.

Nalker, Lisa K. [ASM

2011-11-06T23:59:59.000Z

153

Biofilter for removal of nitrogen oxides from contaminated gases under aerobic conditions  

DOE Patents [OSTI]

A biofilter is described for reducing concentrations of gaseous nitrogen oxides in a polluted gas comprises a porous organic filter bed medium disposed in a housing, the filter bed medium including a mixed culture of naturally occurring denitrifying bacteria for converting the nitrogen oxides to nitrogen gas, carbon dioxide, and water. A method is described of reducing concentrations of nitrogen oxides in polluted gas comprises conducting the polluted gas through the biofilter so that the denitrifying bacteria can degrade the nitrogen oxides. A preferred filter medium is wood compost, however composts of other organic materials are functional. Regulation of pH, moisture content, exogenous carbon sources, and temperature are described. 6 figs.

Apel, W.A.

1998-08-18T23:59:59.000Z

154

Biofilter for removal of nitrogen oxides from contaminated gases under aerobic conditions  

DOE Patents [OSTI]

A biofilter for reducing concentrations of gaseous nitrogen oxides in a polluted gas comprises a porous organic filter bed medium disposed in a housing, the filter bed medium including a mixed culture of naturally occurring denitrifying bacteria for converting the nitrogen oxides to nitrogen gas, carbon dioxide, and water. A method of reducing concentrations of nitrogen oxides in polluted gas comprises conducting the polluted gas through the biofilter so that the denitrifying bacteria can degrade the nitrogen oxides. A preferred filter medium is wood compost, however composts of other organic materials are functional. Regulation of pH, moisture content, exogenous carbon sources, and temperature are described.

Apel, William A. (Idaho Falls, ID)

1998-01-01T23:59:59.000Z

155

Detection of phenols using engineered bacteria  

DOE Patents [OSTI]

Detection of phenols using engineered bacteria. A biosensor can be created by placing a reporter gene under control of an inducible promoter. The reporter gene produces a signal when a cognate transcriptional activator senses the inducing chemical. Creation of bacterial biosensors is currently restricted by limited knowledge of the genetic systems of bacteria that catabolize xenobiotics. By using mutagenic PCR to change the chemical specificity of the Pseudomonas species CF600 DmpR protein, the potential for engineering novel biosensors for detection of phenols has been demonstrated. DmpR, a well-characterized transcriptional activator of the P. CF600's dmp operon mediates growth on simple phenols. Transcription from Po, the promoter heading the dmp operon, is activated when the sensor domain of DmpR interacts with phenol and mono-substituted phenols. By altering the sensor domain of the DmpR, a group of DmpR derivatives that activate transcription of a Po-lacZ fusion in response to eight of the EPA's eleven priority pollutant phenols has been created. The assays and the sensor domain mutations that alter the chemical specificity of DmpR is described.

Wise, Arlene A. (Philadelphia, PA); Kuske, Cheryl R. (Los Alamos, NM); Terwilliger, Thomas C. (Santa Fe, NM)

2007-12-04T23:59:59.000Z

156

acid bacteria reveals: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Diversity and characterization of sulfate-reducing bacteria in groundwater at a uranium mill tailings site CiteSeer Summary: plays a role in both natural attenuation and...

157

acid bacteria inducing: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Summary: The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Clark of amino acid in soil using radioactive isotopes, however many experiments...

158

acid bacteria enhance: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

de 4 The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Environmental Sciences and Ecology Websites Summary: The Fate of Amino Acid in...

159

Leachability of salmonella and fecal pollution indicator bacteria through soil  

E-Print Network [OSTI]

24 6. Percentage of bacteria adsorbed onto soil particles greater than 1 um in diameter 25 7. lhe proportion of bacteria at a given depth that leached thro gh an Arenosa loamy sand 31 8. The proportion of bacteria at a given depth that leached... of the physical regime permitting bacterial movement in soils. They were: bacteria depend on a continuous water pathway where the water filled pores have a greater pore neck diameter than 2 to 3 um, and the lense of water in very large pores must...

Fehrmann, Robert Clinton

2012-06-07T23:59:59.000Z

160

anaerobic phototrophic bacteria: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

bacteria convert organic matter into methane and carbon dioxide (a mixture called biogas) in the absence of air. It is a widely used biological process for treating wastewater...

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

Energy conversion in Purple Bacteria Photosynthesis  

E-Print Network [OSTI]

The study of how photosynthetic organisms convert light offers insight not only into nature's evolutionary process, but may also give clues as to how best to design and manipulate artificial photosynthetic systems -- and also how far we can drive natural photosynthetic systems beyond normal operating conditions, so that they can harvest energy for us under otherwise extreme conditions. In addition to its interest from a basic scientific perspective, therefore, the goal to develop a deep quantitative understanding of photosynthesis offers the potential payoff of enhancing our current arsenal of alternative energy sources for the future. In the following Chapter, we consider the trade-off between dynamics, structure and function of light harvesting membranes in Rps. Photometricum purple bacteria, as a model to highlight the priorities that arise when photosynthetic organisms adapt to deal with the ever-changing natural environment conditions.

Felipe Caycedo-Soler; Ferney J. Rodriguez; Luis Quiroga; Guannan Zhao; Neil F. Johnson

2011-07-01T23:59:59.000Z

162

Energy conversion in Purple Bacteria Photosynthesis  

E-Print Network [OSTI]

The study of how photosynthetic organisms convert light offers insight not only into nature's evolutionary process, but may also give clues as to how best to design and manipulate artificial photosynthetic systems -- and also how far we can drive natural photosynthetic systems beyond normal operating conditions, so that they can harvest energy for us under otherwise extreme conditions. In addition to its interest from a basic scientific perspective, therefore, the goal to develop a deep quantitative understanding of photosynthesis offers the potential payoff of enhancing our current arsenal of alternative energy sources for the future. In the following Chapter, we consider the trade-off between dynamics, structure and function of light harvesting membranes in Rps. Photometricum purple bacteria, as a model to highlight the priorities that arise when photosynthetic organisms adapt to deal with the ever-changing natural environment conditions.

Caycedo-Soler, Felipe; Quiroga, Luis; Zhao, Guannan; Johnson, Neil F

2011-01-01T23:59:59.000Z

163

Field application of a genetically engineered microorganism for polycyclic aromatic hydrocarbon bioremediation process monitoring and control  

SciTech Connect (OSTI)

On October 30, 1996, the US Environmental Protection Agency (EPA) commenced the first test release of genetically engineered microorganisms (GEMs) for use in bioremediation. The specific objectives of the investigation were multifaceted and include (1) testing the hypothesis that a GEM can be successfully introduced and maintained in a bioremediation process, (2) testing the concept of using, at the field scale, reporter organisms for direct bioremediation process monitoring and control, and (3) acquiring data that can be used in risk assessment decision making and protocol development for future field release applications of GEMs. The genetically engineered strain under investigation is Pseudomonas fluorescens strain HK44 (King et al., 1990). The original P. fluorescens parent strain was isolated from polycyclic aromatic hydrocarbon (PAH) contaminated manufactured gas plant soil. Thus, this bacterium is able to biodegrade naphthalene (as well as other substituted naphthalenes and other PAHs) and is able to function as a living bioluminescent reporter for the presence of naphthalene contamination, its bioavailability, and the functional process of biodegradation. A unique component of this field investigation was the availability of an array of large subsurface soil lysimeters. This article describes the experience associated with the release of a genetically modified microorganism, the lysimeter facility and its associated instrumentation, as well as representative data collected during the first eighteen months of operation.

Sayler, G.S.; Cox, C.D.; Ripp, S.; Nivens, D.E.; Werner, C.; Ahn, Y.; Matrubutham, U. [Univ. of Tennessee, Knoxville, TN (United States); Burlage, R. [Oak Ridge National Lab., TN (United States). Environmental Sciences Div.

1998-11-01T23:59:59.000Z

164

E-Print Network 3.0 - aeruginosa biofilm bacteria Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

of bacteria that are held... and secretion of EPSs, but although some bacteria switch on EPS production at high cell densities in biofilms... , other bacteria switch it off. Nadell...

165

The Stabilisation Potential of Individual and Mixed Assemblages of Natural Bacteria and Microalgae  

E-Print Network [OSTI]

The Stabilisation Potential of Individual and Mixed Assemblages of Natural Bacteria and Microalgae components (heterotrophic bacteria and autotrophic microalgae) in terms of their individual contribution benthic assemblages (prokaryotic, eukaryotic and mixed cultures) of bacteria and microalgae. Mag

Paris-Sud XI, Université de

166

Uptake and utilization of dissolved glycine by the polychaete Capitella capitata (Fabricius) under aerobic and anaerobic conditions  

E-Print Network [OSTI]

Results of regression analyses on uptake data, with a Student's t test on the equality of slopes, H : B PB II Results of regression analyses on release data III Per cent distribution of C in C 02 14 . 14 production experiment. Page 26 30 32 IV... UPTAKE AND UTILIZATION OF DISSOLVED GLYCINE BY THE POLYCHAETE CAPITELLA CAPITATA (FABRICIUS) UNDER AEROBIC AND ANAEROBIC CONDITIONS A Thesis by DUANE DESMOND SANDERS Submitted to the Graduate College of Texas A(M University in partial...

Sanders, Duane Desmond

1981-01-01T23:59:59.000Z

167

How sulphate-reducing microorganisms cope with stress: Lessons from systems biology  

SciTech Connect (OSTI)

Sulphate-reducing microorganisms (SRMs) are a phylogenetically diverse group of anaerobes encompassing distinct physiologies with a broad ecological distribution. As SRMs have important roles in the biogeochemical cycling of carbon, nitrogen, sulphur and various metals, an understanding of how these organisms respond to environmental stresses is of fundamental and practical importance. In this Review, we highlight recent applications of systems biology tools in studying the stress responses of SRMs, particularly Desulfovibrio spp., at the cell, population, community and ecosystem levels. The syntrophic lifestyle of SRMs is also discussed, with a focus on system-level analyses of adaptive mechanisms. Such information is important for understanding the microbiology of the global sulphur cycle and for developing biotechnological applications of SRMs for environmental remediation, energy production, biocorrosion control, wastewater treatment and mineral recovery.

Zhou, J.; He, Q.; Hemme, C.L.; Mukhopadhyay, A.; Hillesland, K.; Zhou, A.; He, Z.; Nostrand, J.D. Van; Hazen, T.C.; Stahl, D.A.; Wall, J.D.; Arkin, A.P.

2011-04-01T23:59:59.000Z

168

Environmental assessment of the potential effects of aquifer thermal energy storage systems on microorganisms in groundwater  

SciTech Connect (OSTI)

The primary objective of this study was to evaluate the potential environmental effects (both adverse and beneficials) of aquifer thermal energy storage (ATES) technology pertaining to microbial communities indigenous to subsurface environments (i.e., aquifers) and the propagation, movement, and potential release of pathogenic microorganisms (specifically, Legionella) within ATES systems. Seasonal storage of thermal energy in aquifers shows great promise to reduce peak demand; reduce electric utility load problems; contribute to establishing favorable economics for district heating and cooling systems; and reduce pollution from extraction, refining, and combustion of fossil fuels. However, concerns that the widespread implementation of this technology may have adverse effects on biological systems indigeneous to aquifers, as well as help to propagate and release pathogenic organisms that enter thee environments need to be resolved. 101 refs., 2 tabs.

Hicks, R.J.; Stewart, D.L.

1988-03-01T23:59:59.000Z

169

Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels  

SciTech Connect (OSTI)

The ability to generate microorganisms that can produce biofuels similar to petroleum-based transportation fuels would allow the use of existing engines and infrastructure and would save an enormous amount of capital required for replacing the current infrastructure to accommodate biofuels that have properties significantly different from petroleum-based fuels. Several groups have demonstrated the feasibility of manipulating microbes to produce molecules similar to petroleum-derived products, albeit at relatively low productivity (e.g. maximum butanol production is around 20 g/L). For cost-effective production of biofuels, the fuel-producing hosts and pathways must be engineered and optimized. Advances in metabolic engineering and synthetic biology will provide new tools for metabolic engineers to better understand how to rewire the cell in order to create the desired phenotypes for the production of economically viable biofuels.

Kuk Lee, Sung; Chou, Howard; Ham, Timothy S.; Soon Lee, Taek; Keasling, Jay D.

2009-12-02T23:59:59.000Z

170

Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity  

DOE Patents [OSTI]

This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70.degree. C. to 90.degree. C., at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%.

Premuzic, Eugene T. (East Moriches, NY); Lin, Mow (Rocky Point, NY)

1996-02-20T23:59:59.000Z

171

Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity  

DOE Patents [OSTI]

This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70 C to 90 C, at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%. 68 figs.

Premuzic, E.T.; Lin, M.

1996-02-20T23:59:59.000Z

172

The Aerobic Oxidation of Bromide to Dibromine Catalyzed by Homogeneous Oxidation Catalysts and Initiated by Nitrate in Acetic Acid  

SciTech Connect (OSTI)

A small amount of nitrate, ~0.002 molal, initiates the Co/Mn catalyzed aerobic oxidation of bromide compounds (HBr,NaBr,LiBr) to dibromine in acetic acid at room temperature. At temperatures 40oC or less , the reaction is autocatalytic. Co(II) and Mn(II) themselves and mixed with ionic bromide are known homogeneous oxidation catalysts. The reaction was discovered serendipitously when a Co/Br and Co/Mn/Br catalyst solution was prepared for the aerobic oxidation of methyaromatic compounds and the Co acetate contained a small amount of impurity i.e. nitrate. The reaction was characterized by IR, UV-VIS, MALDI and EXAFS spectroscopies and the coordination chemistry is described. The reaction is inhibited by water and its rate changed by pH. The change in these variables, as well as others, are identical to those observed during homogeneous, aerobic oxidation of akylaromatics. A mechanism is proposed. Accidental addition of a small amount of nitrate compound into a Co/Mn/Br/acetic acid mixture in a large, commercial feedtank is potentially dangerous.

Partenheimer, Walt; Fulton, John L.; Sorensen, Christina M.; Pham, Van Thai; Chen, Yongsheng

2014-06-01T23:59:59.000Z

173

Determination of kinetic coefficients for the reduction and removal of uranium from water by the Desulfovibrio desulfuricans bacteria  

SciTech Connect (OSTI)

Uranium contamination of groundwater and surface water from abandoned uranium mill tailings piles is a serious concern in many areas of the western United States. U(VI) is soluble in water and, as a result, is relatively mobile in the environment. U(IV), however, is generally insoluble in water and, therefore, is not subject to aqueous transport. In recent years, researchers have discovered that certain microorganisms, such as the sulfate-reducing bacteria Desuffiovibrio desulfricans, can mediate the reduction of U(VI) to U(IV) by anaerobic respiration. Although the ability of this microorganism to reduce U(VI) has been studied in some detail by previous researchers, the kinetics of the reaction have not been characterized. The purpose of this research was to perform kinetic studies on Desuffiovibrio desulfricans during simultaneous reduction of sulfate and uranium and to determine the mineral phase of uranium after it has been reduced. The studies were conducted in a laboratory-scale chemostat under substrate-limited growth conditions with pyruvate as the substrate. The maximum rate of substrate utilization (k) was determined to be 4.70 days{sup -1} while the half-velocity constant (Ks) was 140 mg CODA. The yield coefficient (Y) was determined to be 0. 17 mg cells/mg COD while the endogenous decay coefficient (kd) was found to be 0.072 days{sup -1}. After reduction, U(IV) precipitated from solution in the uraninite (UO{sub 2}) phase as predicted by thermodynamics. Uranium removal efficiency as high as 90% was achieved in the chemostat.

Tucker, M.D.; Barton, L.L.; Thomson, B.M. [Sandia National Labs., Albuquerque, NM (United States)

1996-12-31T23:59:59.000Z

174

Characterizing the transformation and transfer of nitrogen during the aerobic treatment of organic wastes and digestates  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer Ammonia emissions varied depending on the nature of wastes and the treatment conditions. Black-Right-Pointing-Pointer Nitrogen losses resulted from ammonia emissions and nitrification-denitrification. Black-Right-Pointing-Pointer Ammonification can be estimated from biodegradable carbon and carbon/nitrogen ratio. Black-Right-Pointing-Pointer Ammonification was the main process contributing to N losses. Black-Right-Pointing-Pointer Nitrification rate was negatively correlated to stripping rate of ammonia nitrogen. - Abstract: The transformation and transfer of nitrogen during the aerobic treatment of seven wastes were studied in ventilated air-tight 10-L reactors at 35 Degree-Sign C. Studied wastes included distinct types of organic wastes and their digestates. Ammonia emissions varied depending on the kind of waste and treatment conditions. These emissions accounted for 2-43% of the initial nitrogen. Total nitrogen losses, which resulted mainly from ammonia emissions and nitrification-denitrification, accounted for 1-76% of the initial nitrogen. Ammonification was the main process responsible for nitrogen losses. An equation which allows estimating the ammonification flow of each type of waste according to its biodegradable carbon and carbon/nitrogen ratio was proposed. As a consequence of the lower contribution of storage and leachate rates, stripping and nitrification rates of ammonia nitrogen were negatively correlated. This observation suggests the possibility of promotingnitrification in order to reduce ammonia emissions.

Zeng Yang, E-mail: yang.zeng@irstea.fr [Irstea, UR GERE, 17 avenue de Cucille, CS 64427, F-35044 Rennes Cedex (France); Universite Europeenne de Bretagne, F-35000 Rennes (France); Guardia, Amaury de; Daumoin, Mylene; Benoist, Jean-Claude [Irstea, UR GERE, 17 avenue de Cucille, CS 64427, F-35044 Rennes Cedex (France)

2012-12-15T23:59:59.000Z

175

Intrinsic bioremediation of a BTEX and MTBE plume under mixed aerobic/denitrifying conditions  

SciTech Connect (OSTI)

A shallow Coastal Plain aquifer in rural Sampson Country, North Carolina, has been contaminated with petroleum hydrocarbon from a leaking underground storage tank containing gasoline.An extensive field characterization has been performed to define the horizontal and vertical distribution of soluble gasoline components and indicator parameters. A plume of dissolved methyl tert-butyl ether (MTBE) and the aromatic hydrocarbons benzene, toluene, ethylbenzene, and xylene isomers (BTEX) is present in the aquifer and has migrated over 600 ft from the source area. Background dissolved oxygen concentrations range from 7 to 8 mg/L, and nitrate concentrations range from 5 to 22 mg/L as N due to extensive fertilization of fields surrounding the spill. In the center of the BTEX plume, oxygen concentrations decline to less than 1 mg/L while nitrate concentrations remain high. The total mass flux of MTBE and all BTEX components decline with distance downgradient relative to a conservative tracer (chloride). At the source, the total BTEX concentration exceeds 75 mg/L while 130 ft downgradient, total BTEX concentrations are less than 4.9 mg/L, a 15-fold reduction. Toluene and ethylbenzene decline most rapidly followed by m-p-xylene, o-xylene and finally benzene. Biodegradation of TEX appears to be enhanced by the excess nitrate present in the aquifer while benzene biodegradation appears to be due to strictly aerobic processes.

Borden, R.C.; Daniel, R.A. [North Carolina State Univ., Raleigh, NC (United States). Civil Engineering Dept.

1995-09-01T23:59:59.000Z

176

Bacteria Total Maximum Daily Load Task Force Final Report  

E-Print Network [OSTI]

for TMDL and Watershed Studies at Virginia Tech (http://www.tmdl.bse.vt.edu/outreach/C85/). Bacteria Indicator Tool (BIT) Another MB tool is the BIT provided by EPA (http://www.epa.gov/waterscience/ftp/basins/system/BASINS3/bit.htm). The BIT is a... spreadsheet that can be used to estimate the monthly accumulation rate of fecal coliform bacteria on four land uses (cropland, forested, built-up and pastureland). The tool also estimates the direct input of fecal coliform bacteria to streams from grazing...

Jones, C. Allan; Wagner, Kevin; Di Giovanni, George; Hauck, Larry; Mott, Joanna; Rifai, Hanadi; Srinivasan, Raghavan; Ward, George; Wythe, Kathy

177

Beating bacteria: Scientists work to understand and track bacteria in water  

E-Print Network [OSTI]

to Tier 2. Tier 2 is a one- to two-year effort with the follow- ing steps: 1) Collect targeted monitoring data to fill gaps in previously collected data 2) Conduct qualitative library-independent BST and limited library-dependent BST analysis... to determine whether humans and/or a few major classes of animals are sources 3) Develop simple spatially explicit or mass balance models of bacteria in the watershed 4) Analyze data After analysis of Tier 1 and Tier 2 data, the group may chose...

Wythe, Kathy

2010-01-01T23:59:59.000Z

178

E-Print Network 3.0 - acid-tolerant thermophilic bacteria Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Homoacetogenic bacteria - often called acetogenic' bacteria... .1.4 Selection of methanol as electron donor 6 1.1.5 Selection ... Source: Groningen, Rijksuniversiteit -...

179

Thermophilic and hyperthermophilic microorganisms in 3^30C hydrothermal uids following a deep-sea volcanic eruption  

E-Print Network [OSTI]

25 (1998) 33^41 #12;£oor. Hyperthermophilic microorganisms have also recently been isolated from oil reservoirs in the North Sea, in the north slope of Alaska, and in continental reservoirs in France [5^7]. The lack of exogenous seawater in the French reservoirs, which could act as a source of hyperthermophile

Holden, James F.

180

Advanced Biofuels: How Scientists are Engineering Bacteria to...  

Energy Savers [EERE]

- 2:12pm Addthis Strains of E. coli bacteria were engineered to digest switchgrass biomass and synthesize its sugars into gasoline, diesel and jet fuel. | Image courtesy of...

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

Micro-scale interactions between chemotactic bacteria and algae  

E-Print Network [OSTI]

Traditional views of marine environments describe the ocean pelagic zone as a homogeneous nutrient-poor environment. Heterotrophic marine bacteria that have evolved high-energy mechanisms for swimming abilities and sensing ...

Vahora, Nisha

2010-01-01T23:59:59.000Z

182

Biodegradation of bisphenol a and ibuprofen by ammonia oxidizing bacteria  

E-Print Network [OSTI]

might be responsible for the removal of these compounds. Several bacterial strains, isolated from waste water, are known to degrade BPA and ibuprofen. No studies, however, have reported using ammonia oxidizing bacteria for this purpose. Ammonia oxidizing...

Subramanya, Nethra T.

2007-09-17T23:59:59.000Z

183

Foodborne Sources of Bacteria Associated With Human Obesity  

E-Print Network [OSTI]

Recently published research has suggested that the microbial ecology of the digestive system may play a role in obesity. Obese people have been shown to have a higher proportion of bacteria from the Firmicutes division and a lower proportion...

McElhany, Katherine Grace

2009-09-30T23:59:59.000Z

184

Rapid identification of bacteria using an umbelliferone fluorescent assay  

E-Print Network [OSTI]

RAPID IDENTIFICATION OF BACTERIA USING AN UMBELLIFERONE FLUORESCENT ASSAY A Thesis by RICHARD THOMAS CHAMBLIN, JR, Submitted to the Graduate College of' Texas A&M University in partial fulfillment of the requirement for the degree of MASTER... OF SCIENCE August 1983 Major Subject: Veterinary Microbiology RAPID IDENTIFICATION OF BACTERIA USING AN UMBELLIFERONE FLUORESCENT ASSAY A Thesis by RICHARD THOMAS CHAMBLIN, JR ~ Approved as to style and content by ( -Chairman of Committee) John M...

Chamblin, Richard Thomas

1983-01-01T23:59:59.000Z

185

Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702  

DOE Patents [OSTI]

Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

Dees, H.C.

1998-05-26T23:59:59.000Z

186

Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702  

DOE Patents [OSTI]

Bacteria which produce large amounts of cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

Dees, H.C.

1997-12-16T23:59:59.000Z

187

Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702  

DOE Patents [OSTI]

Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

Dees, H. Craig (Lenoir City, TN)

1998-01-01T23:59:59.000Z

188

Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702  

DOE Patents [OSTI]

Bacteria which produce large amounts of cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

Dees, H. Craig (Lenoir City, TN)

1997-12-16T23:59:59.000Z

189

Nutrient leaching following land application of aerobically digested municipal sewage sludge in a northern hardwood forest  

SciTech Connect (OSTI)

Concentrations of selected cations and anions were determined in soil solutions from a forested site in southern New Hampshire during the first growing season after surface application of an aerobically digested, limed, liquid municipal sludge. Sludge was applied in June 1989 at 0, 3.3, 6.9, and 14.5 Mg ha{sup -1}, which corresponded to 199, 396, and 740 kg N ha{sup -1} as total Kjeldahl N (TKN). Porous, suction-cup lysimeters were used to sample soil solutions below the rooting zone ({approximately}60 cm) within subplots designed to include (untrenched) or exclude (trenched) uptake by vegetation. Following sludge application, measured solute concentrations remained low until September 1989, when NO{sub 3}, Cl, Ca, Mg, Na, and K in trenched subplots increased simultaneously to maximum values in October or November 1989, just before the soil froze for the winter. Nitrate was the dominant anion in soil solutions from trenched subplots and averaged in excess of 0.71 mmol L{sup -1} (10 mg L{sup -1} NO{sub 3}-N) at all loading rates. Highest concentrations of NO{sub 3} occurred on subplots with the highest sludge application rates. In the entrenched areas, NO{sub 3} concentrations rarely exceeded 0.001 mmol L{sup -1}; Cl increased in treated areas and was the dominant anion by the end of the season. Soil solution NH{sub 4}, PO{sub 4}, SO{sub 4} K, and pH did not change significantly for any sludge application rate. Comparison of results from trenched and untrenched areas suggests that, at application rates of up to 14.5 Mg ha{sup -1 }(799 kg TKN ha{sup -1}), a combination of physical, chemical, and biological factors (most likely plant uptake) limited the movement of sludge or sludge-derived constituents from the sites of application. 37 refs., 8 figs., 3 tabs.

Medalie, L.; Bowden, W.B.; Smith, C.T. [Univ. of New Hampshire, Durham, NH (United States)

1994-01-01T23:59:59.000Z

190

A study of the localized humoral immune response to implicated microorganisms in juvenile periodontitis  

SciTech Connect (OSTI)

A study was undertaken using an in vitro explant culture system to determine the presence of immunoglobulins (IgG, IgA, and IgM) in the supernatant fluids (SF) of disease gingival tissue explant cultures. Studies were also undertaken to determine if the de novo biosynthesis of {sup 14}C-immunoglobulins could be observed in the explant cultures of diseased tissues from juvenile periodontitis (JP) patients. Radiolabeled proteins were detected in the SF and immunodiffusion studies using goat antihuman gamma, alpha or mu chain serum revealed the presence of IgG and IgA but no IgM present in the SF of the JP gingival tissue explant cultures. Immunodiffusion studies using goat anti-human gamma chain serum with Staph protein A isolated IgG fractions of the SF, followed by autoradiography of the IgG precipitation lines demonstrated the biosynthesis of IgG by the JP gingival tissue explant cultures. The serological studies suggested that local immune response in JP was to a polymicrobic infection. The SF of JP showed significantly higher levels of antibody reactivity to B. intermedius, C. ochracea, E. nodatum and P. micros as compared to healthy tissues. The local antibody response to the microorganisms tested differed from that observed in the sera of the patients.

Hall, E.R.

1988-01-01T23:59:59.000Z

191

An Approach for Assessing the Signature Quality of Various Chemical Assays when Predicting the Culture Media Used to Grow Microorganisms  

SciTech Connect (OSTI)

We demonstrate an approach for assessing the quality of a signature system designed to predict the culture medium used to grow a microorganism. The system was comprised of four chemical assays designed to identify various ingredients that could be used to produce the culture medium. The analytical measurements resulting from any combination of these four assays can be used in a Bayesian network to predict the probabilities that the microorganism was grown using one of eleven culture media. We evaluated combinations of the signature system by removing one or more of the assays from the Bayes network. We measured and compared the quality of the various Bayes nets in terms of fidelity, cost, risk, and utility, a method we refer to as Signature Quality Metrics

Holmes, Aimee E.; Sego, Landon H.; Webb-Robertson, Bobbie-Jo M.; Kreuzer, Helen W.; Anderson, Richard M.; Unwin, Stephen D.; Weimar, Mark R.; Tardiff, Mark F.; Corley, Courtney D.

2013-02-01T23:59:59.000Z

192

Influence of microorganisms on the oxidation state distribution of multivalent actinides under anoxic conditions  

SciTech Connect (OSTI)

The fate and potential mobility of multivalent actinides in the subsurface is receiving increased attention as the DOE looks to cleanup the many legacy nuclear waste sites and associated subsurface contamination. Plutonium, uranium and neptunium are the near-surface multivalent contaminants of concern and are also key contaminants for the deep geologic disposal of nuclear waste. Their mobility is highly dependent on their redox distribution at their contamination source as well as along their potential migration pathways. This redox distribution is often controlled, especially in the near-surface where organic/inorganic contaminants often coexist, by the direct and indirect effects of microbial activity. Under anoxic conditions, indirect and direct bioreduction mechanisms exist that promote the prevalence of lower-valent species for multivalent actinides. Oxidation-state-specific biosorption is also an important consideration for long-term migration and can influence oxidation state distribution. Results of ongoing studies to explore and establish the oxidation-state specific interactions of soil bacteria (metal reducers and sulfate reducers) as well as halo-tolerant bacteria and Archaea for uranium, neptunium and plutonium will be presented. Enzymatic reduction is a key process in the bioreduction of plutonium and uranium, but co-enzymatic processes predominate in neptunium systems. Strong sorptive interactions can occur for most actinide oxidation states but are likely a factor in the stabilization of lower-valent species when more than one oxidation state can persist under anaerobic microbiologically-active conditions. These results for microbiologically active systems are interpreted in the context of their overall importance in defining the potential migration of multivalent actinides in the subsurface.

Reed, Donald Timothy [Los Alamos National Laboratory; Borkowski, Marian [Los Alamos National Laboratory; Lucchini, Jean - Francois [Los Alamos National Laboratory; Ams, David [Los Alamos National Laboratory; Richmann, M. K. [Los Alamos National Laboratory; Khaing, H. [Los Alamos National Laboratory; Swanson, J. S. [Los Alamos National Laboratory

2010-12-10T23:59:59.000Z

193

Biodesulfurization techniques: Application of selected microorganisms for organic sulfur removal from coals. Final report  

SciTech Connect (OSTI)

As an alternative to post-combustion desulfurization of coal and pre-combustion desulfurization using physicochemical techniques, the microbial desulfurization of coal may be accomplished through the use of microbial cultures that, in an application of various microbial species, may remove both the pyritic and organic fractions of sulfur found in coal. Organisms have been isolated that readily depyritize coal but often at prohibitively low rates of desulfurization. Microbes have also been isolated that may potentially remove the organic-sulfur fraction present in coal (showing promise when acting on organic sulfur model compounds such as dibenzothiophene). The isolation and study of microorganisms demonstrating a potential for removing organic sulfur from coal has been undertaken in this project. Additionally, the organisms and mechanisms by which coal is microbially depyritized has been investigated. Three cultures were isolated that grew on dibenzothiophene (DBT), a model organic-sulfur compound, as the sole sulfur source. These cultures (UMX3, UMX9, and IGTS8) also grew on coal samples as the sole sulfur source. Numerous techniques for pretreating and ``cotreating`` coal for depyritization were also evaluated for the ability to improve the rate or extent of microbial depyritization. These include prewashing the coal with various solvents and adding surfactants to the culture broth. Using a bituminous coal containing 0.61% (w/w) pyrite washed with organic solvents at low slurry concentrations (2% w/v), the extent of depyritization was increased approximately 25% in two weeks as compared to controls. At slurry concentrations of 20% w/v, a tetrachloroethylene treatment of the coal followed by depyritization with Thiobacillus ferrooxidans increased both the rate and extent of depyritization by approximately 10%.

Elmore, B.B.

1993-08-01T23:59:59.000Z

194

Bacteria transport through porous media. Annual report, December 31, 1984  

SciTech Connect (OSTI)

The following five chapters in this report have been processed separately for inclusion in the Energy Data Base: (1) theoretical model of convective diffusion of motile and non-motile bacteria toward solid surfaces; (2) interfacial electrochemistry of oxide surfaces in oil-bearing sands and sandstones; (3) effects of sodium pyrophosphate additive on the ''huff and puff''/nutrient flooding MEOR process; (4) interaction of Escherichia coli B, B/4, and bacteriophage T4D with Berea sandstone rock in relation to enhanced oil recovery; and (5) transport of bacteria in porous media and its significance in microbial enhanced oil recovery.

Yen, T.F.

1986-09-01T23:59:59.000Z

195

Energy transfer in an LH4-like light harvesting complex from the aerobic purple photosynthetic bacterium Roseobacter denitrificans  

SciTech Connect (OSTI)

A peripheral light-harvesting complex from the aerobic purple bacterium Roseobacter (R.) denitrificans was purified and its photophysical properties characterized. The complex contains two types of pigments, bacteriochlorophyll (BChl) a and the carotenoid (Car) spheroidenone and possesses unique spectroscopic properties. It appears to lack the B850 bacteriochlorophyll a Q{sub y} band that is typical for similar light-harvesting complex 2 antennas. Circular dichroism and low temperature steady-state absorption spectroscopy revealed that the B850 band is present but is shifted significantly to shorter wavelengths and overlaps with the B800 band at room temperature. Such a spectral signature classifies this protein as a member of the light-harvesting complex 4 class of peripheral light-harvesting complexes, along with the previously known light-harvesting complex 4 from Rhodopseudomonas palustris. The influence of the spectral change on the light-harvesting ability was studied using steady-state absorption, fluorescence, circular dichroism, femtosecond and microsecond time-resolved absorption and time-resolved fluorescence spectroscopies. The results were compared to the properties of the similar (in pigment composition) light-harvesting complex 2 from aerobically grown Rhodobacter sphaeroides and are understood within the context of shared similarities and differences and the putative influence of the pigments on the protein structure and its properties.

Niedzwiedzki, Dariusz; Fuciman, Marcel; Frank, Harry A; Blankenship, R. E.

2011-01-01T23:59:59.000Z

196

Use of Advanced Oxidation and Aerobic Degradation for Remediation of Various Hydrocarbon Contaminates  

SciTech Connect (OSTI)

Western Research Institute in conjunction with Sierra West Consultants, Inc., Tetra Tech, Inc., and the U.S. Department of Energy conducted laboratory and field studies to test different approaches to enhance degradation of hydrocarbons and associated contaminants. WRI in conjunction with Sierra West Consultants, Inc., conducted a laboratory and field study for using ozone to treat a site contaminated with MTBE and other hydrocarbons. Results from this study demonstrate that a TOD test can be used to resolve the O{sub 3} dosage problem by establishing a site-specific benchmark dosage for field ozone applications. The follow-up testing of the laboratory samples provided indications that intrinsic biodegradation could be stimulated by adding oxygen. Laboratory studies also suggests that O3 dosage in the full-scale field implementation could be dialed lower than stoichiometrically designed to eliminate the formation of Cr(VI). WRI conducted a study involving a series of different ISCO oxidant applications to diesel-contaminated soil and determined the effects on enhancing biodegradation to degrade the residual hydrocarbons. Soils treated with permanganate followed by nutrients and with persulfate followed by nutrients resulted in the largest decrease in TPH. The possible intermediates and conditions formed from NOM and TPH oxidation by permanganate and activated persulfate favors microbial TPH degrading activity. A 'passive-oxidation' method using microbial fuel cell (MFC) technology was conducted by WRI in conjunction with Tetra Tech, Inc., to degrade MTBE in groundwater. These experiments have demonstrated that a working MFC (i.e., one generating power) could be established in the laboratory using contaminated site water or buffered media inoculated with site water and spiked with MTBE, benzene, or toluene. Electrochemical methods were studied by WRI with goal of utilizing low voltage and amperage electrical sources for 'geo-oxidation' of organic contaminants. The results from a study with TCE contaminated-clay indicate that electrochemically inducing reductive dechlorination of TCE in a saturated matrix may offer an effective and viable alternative to remediation TCE and other contaminants with potential of being reduced. Another study focused on steel wool oxidation to electrochemically increase population of hydrocarbon-degrading denitrifying bacteria. Significantly larger denitrifying activity was observed in the cathode chamber of a treatment unit setup like an MFC with steel wool as the anode. This enhanced nitrate reduction could be due to direct electron utilization by denitrifying bacteria on the cathode, thereby stimulating microbial denitrification or a combination of electron transfer directly to NO{sub 3}{sup -} and electron transfer to nitrate reducing bacteria, which may serve as a type of bio-catalyst on the cathode for nitrate reduction. Overall, the studies conducted under Task 72 demonstrated different innovative methods to enhance petroleum hydrocarbon degradation and associated contaminants.

Paul Fallgren

2009-03-06T23:59:59.000Z

197

Films of bacteria at interfaces: three stages of behaviour  

E-Print Network [OSTI]

Bacterial attachment to a fluid interface can lead to the formation of a film with physicochemical properties that evolve with time. We study the time evolution of interface (micro)mechanics for interfaces between oil and bacterial suspensions by following the motion of colloidal probes trapped by capillarity to determine the interface microrheology. Initially, active bacteria at and near the interface drive superdiffusive motion of the colloidal probes. Over timescales of minutes, the bacteria form a viscoelastic film which we discuss as a quasi-two-dimensional, active, glassy system. To study late stage mechanics of the film, we use pendant drop elastometry. The films, grown over tens of hours on oil drops, are expanded and compressed by changing the drop volume. For small strains, by modeling the films as 2D Hookean solids, we estimate the film elastic moduli, finding values similar to those reported in the literature for the bacteria themselves. For large strains, the films are highly hysteretic. Finally, from wrinkles formed on highly compressed drops, we estimate film bending energies. The dramatic restructuring of the interface by such robust films has broad implications, e.g. in the study of active colloids, in understanding the community dynamics of bacteria, and in applied settings including bioremediation.

Liana Vaccari; Daniel Allan; Nima Sharifi-Mood; Aayush Singh; Robert Leheny; Kathleen Stebe

2015-03-25T23:59:59.000Z

198

Magnetic Torque of Microfabricated Elements and Magnetotactic Bacteria  

E-Print Network [OSTI]

We present a thorough theoretical analysis of the magnetic torque on microfabricated elements with dimensions in the range of 100 to 500 {\\mu}m and magneto-somes of magnetotactic bacteria of a few {\\mu}m length. We derive simple equations for field dependent torque and magnetic shape anisotropy that can be readily used to replace the crude approximations commonly used. We illustrate and verify the theory on microfabricated elements and magnetotactic bacteria, by field depedent torque magnetometry and by observing their rotation in water under application of a rotating magnetic field. The maximum rotation frequency of the largest microfabricated elements agrees within error boundaries with theory. For smaller, and especially thinner, elements the measured frequencies are a factor of three to four too low. We suspect this is caused by incomplete saturation of the magnetisation in the elements, which is not incorporated in our model. The maximum rotation frequency of magnetotactic bacteria agrees with our model within error margins, which are however quite big due to the large spread in bacteria morphology. The model presented provides a solid basis for the analysis of experiments with magnetic objects in liquid, which is for instance the case in the field of medical microrobotics.

Lars Zondervan; Özlem Sardan Sukas; Islam S. M. Khalil; Marc P. Pichel; Sarthak Misra; Leon Abelmann

2014-08-07T23:59:59.000Z

199

CARD-FISH and Microautoradiography Protocol for Bacteria and Archaea  

E-Print Network [OSTI]

1 CARD-FISH and Microautoradiography Protocol for Bacteria and Archaea by Gerhard Herndl Lab @ www FISH Hybridization www.microbial-oceanography.eu 2007 2 Sample Fixation 1. Fix samples in Ethanol 95% 7. Dry and mount in DAPI mix #12;CARD FISH Buffers and Chemicals www

Herndl, Gerhard

200

Methods for targetted mutagenesis in gram-positive bacteria  

SciTech Connect (OSTI)

The present invention provides a method of targeted mutagenesis in Gram-positive bacteria. In particular, the present invention provides a method that effectively integrates a suicide integrative vector into a target gene in the chromosome of a Gram-positive bacterium, resulting in inactivation of the target gene.

Yang, Yunfeng

2014-05-27T23:59:59.000Z

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

Method for establishing the presence of salmonella bacteria in eggs  

DOE Patents [OSTI]

Measurement of the acoustical resonances in eggs is shown to provide a rapid, noninvasive technique for establishing the presence of Salmonella bacteria. The technique is also sensitive to yolk puncture, shell cracks, and may be sensitive to other yolk properties and to egg freshness. Remote characterization, potentially useful for characterizing large numbers of eggs, has been demonstrated.

Johnston, Roger G. (Los Alamos, NM); Sinha, Dipen N. (Los Alamos, NM)

1995-01-01T23:59:59.000Z

202

Protein signaling via type III secretion pathways in phytopathogenic bacteria  

E-Print Network [OSTI]

-5274 Abbreviations HR hypersensitive response Pv pathovar NLS nuclear localization signals Introduction Plants secretion pathway has revealed new mechanisms by which phytopathogenic bacteria infect plants. The suggestion that bacterial gene products are `delivered to' and `perceived by' plants cells has fundamentally

Mudgettt, Mary Beth

203

Bacteria beneath the West Antarctic Ice Sheet Brian Lanoil,1  

E-Print Network [OSTI]

Bacteria beneath the West Antarctic Ice Sheet Brian Lanoil,1 * Mark Skidmore,1 John C. Priscu,2, particularly those that lie beneath polar ice sheets, are beginning to be recog- nized as an important part Vostok, Antarctica, no sub-ice sheet environments have been shown to support microbial ecosystems. Here

Priscu, John C.

204

Introduction The use of immobilized bacteria for ex situ bioremedi-  

E-Print Network [OSTI]

). Foam embedded bacteria (0.1 g) or equivalent mass of free cells (0.59 ml) were transferred to glass polyurethane-based foams to immobilize algae for metal removal (Wilde et al., 1997), and, more recently et al., 1989) to appropriate concentrations. Foams consisted of 20 g hydrophilic polyurethane pre

Hazen, Terry

205

Physiologically anaerobic microorganisms of the deep subsurface. Final performance report, June 1, 1990--August 31, 1993  

SciTech Connect (OSTI)

Anaerobic bacteria were isolated from deep subsurface sediment samples taken at study sites in Idaho (INEL) and Washington (HR) by culturing on dilute and concentrated medium. Morphologically distinct colonies were purified, and their responses to 21 selected physiological tests were determined. Although the number of isolates was small (18 INEL, 27 HR) some general patterns could be determined. Most strains could utilize all the carbon sources, however the glycerol and melizitose utilization was positive for 50% or less of the HR isolates. Catalase activity (27.78% at INEL, 74.07% at HR) and tryptophan metabolism (11.12% at INEL, 40.74% at HR) were significantly different between the two study sites. MPN and viable counts indicate that sediments near the water table yield the greatest numbers of anaerobes. Deeper sediments also appear to be more selective with the greatest number of viable counts on low-nutrient mediums. Likewise, only strictly obligate anaerobes were found in the deepest sediment samples. Selective media indicated the presence of methanogens, acetogens, and sulfate reducers at only the HR site.

Stevens, S.E. Jr.; Chung, K.T.

1993-10-01T23:59:59.000Z

206

Inhibition of X-ray-induced potentially lethal damage (PLD) repair in aerobic plateau-phase Chinese hamster cells by misonidazole  

SciTech Connect (OSTI)

The effect of the 2-nitroimidazole radiosensitizer misonidazole (MISO) and the hydrophilic analog SR-2508 on the repair of X-ray-induced potentially lethal damage (PLD) was studied in plateau-phase Chinese Hamster ovary (HA-1) cells. It was found that although MISO does not radiosensitize aerobic cells, it inhibits the repair of PLD. However, under hypoxic conditions, MISO has no effect on PLD repair. The major portion of the inhibition of PLD repair in aerobic cells requires the presence of MISO only during irradiation; little or no additional inhibition occurs when MISO is present during the postirradiation repair period. Also, treatment of aerobic cells with 5 mM MISO for either 5 or 30 min prior to irradiation is equally inhibitory. This suggests that the presence of MISO in some way modifies the initial lesion under aerobic conditions since it does not increase cell killing as determined by immediate plating but inhibits subsequent repair. The inhibition is concentration dependent; 0.5 mM MISO inhibits PLD repair by one-half while 5-10 mM totally inhibits the repair measured 6 hr postirradiation. This phenomenon suggests that radiosensitization of tissue in vivo by MISO and other 2-nitroimidazoles may not be unequivocal proof of the presence of hypoxic cells.

Brown, D.M.

1984-01-01T23:59:59.000Z

207

Biofilms as sources of fecal bacteria contamination in the stormwater drainage system in Singapore  

E-Print Network [OSTI]

A study was performed to examine a possible source of fecal bacteria contamination originating from within the stormwater drainage system in Singapore. The extent of fecal bacteria presence in storm drain biofilms was ...

Burkhart, Tsung Hwa (Tsung Hwa Sophia)

2013-01-01T23:59:59.000Z

208

E-Print Network 3.0 - alimentary tract bacteria Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Administered Non-Pathogenic Bacteria 12:20-12:35 Presentation 5 - Dawn Kelly: ,,Optimising... -positive bacteria 12;15:45-16:15 Coffee and Posters 16:15- 17:55 Gut Health...

209

Neem extract as an inhibitor for biocorrosion influenced by sulfate reducing bacteria: A preliminary investigation  

E-Print Network [OSTI]

-reducing bacteria (SRB), iron and CO2 reducing bacteria and iron and manganese oxidizing bacteria [1,2]. Among these were characterized with field emission scanning electron microscopy (FE-SEM) coupled with energy of water for cellular maintenance and growth [4], (5) couple sulfate reduction to the intracellular

210

Reduction of trichloroethylene in a model aquifer with methanotrophic bacteria  

E-Print Network [OSTI]

REDUCTION OF TRICHLOROETHYLENE IN A MODEL AQUIFER WITH METHANOTROPHIC BACTERIA A Thesis by Duane Dee Hicks Submitted to the Office of Graduate Studies of Texas ARM University in partial fullfillment of the requirements for thc degree... of MASTER OF SCIENCE December 1990 Major Subject: Civil Engineering REDUCTION OF TRICHLOROETHYLENE IN A MODEL AQUIFER WITH METHANOTROPHIC BACTEPslA A Thesis by Duane Dec Hicks Approved as to style and content by Bill Batchclor (Chair of Committee...

Hicks, Duane Dee

1990-01-01T23:59:59.000Z

211

Effect of electrical stimulation on bacteria of meats  

E-Print Network [OSTI]

1979 ABSTRACT Effect of Electrical Stimulation on Bacteria of meats. (December 1979) Boosya Mrigadat, B. S. , Kasetsart University Chairman of Advisory Committee: Dr. C. Vanderzant Electrostimulation of rabbit muscles caused a slight reduction... stimulation and after storage for up to 45 min. (CON = open bars, ES = shaded bars; muscles with identical numbers were from the same rabbit). Effect of electrical stimulation on the bacterial count of inoculated (Lacto- b 'll . ) bb't 1 (LD=~L dorsi, BF...

Mrigadat, Boosya

1979-01-01T23:59:59.000Z

212

Oxidation and methylation of dissolved elemental mercury by anaerobic bacteria  

SciTech Connect (OSTI)

Methylmercury is a neurotoxin that poses significant health risks to humans. Some anaerobic sulphate- and iron-reducing bacteria can methylate oxidized forms of mercury, generating methylmercury1-4. One strain of sulphate-reducing bacteria (Desulfovibrio desulfuricans ND132) can also methylate elemental mercury5. The prevalence of this trait among different bacterial strains and species remains unclear, however. Here, we compare the ability of two strains of the sulphate-reducing bacterium Desulfovibrio and one strain of the iron-reducing bacterium Geobacter to oxidise and methylate elemental mercury in a series of laboratory incubations. Experiments were carried out under dark, anaerobic conditions, in the presence of environmentally-relevant concentrations of elemental mercury. We report differences in the ability of these organisms to oxidise and methylate elemental mercury. In line with recent findings5, we show that Desulfovibrio desulfuricans ND132 can both oxidise and methylate elemental mercury. However, the rate of methylation of elemental mercury is only about one third the rate of methylation of oxidized mercury. We also show that Desulfovibrio alaskensis G20 can oxidise, but not methylate, elemental mercury. Geobacter sulfurreducens PCA is able to oxidise and methylate elemental mercury in the presence of cysteine. We suggest that the activity of methylating and non-methylating bacteria may together enhance the formation of methylmercury in anaerobic environments.

Hu, Haiyan [ORNL] [ORNL; Lin, Hui [ORNL] [ORNL; Zheng, Wang [ORNL] [ORNL; Tomanicek, Stephen J [ORNL] [ORNL; Johs, Alexander [ORNL] [ORNL; Feng, Xinbin [ORNL] [ORNL; Elias, Dwayne A [ORNL] [ORNL; Liang, Liyuan [ORNL] [ORNL; Liang, Liyuan [ORNL] [ORNL; Gu, Baohua [ORNL] [ORNL

2013-01-01T23:59:59.000Z

213

The future is now: single-cell genomics of bacteria and archaea  

E-Print Network [OSTI]

Interest in the expanding catalog of uncultivated microorganisms, increasing recognition of heterogeneity among seemingly similar cells, and technological advances in whole-genome amplification and single-cell manipulation ...

Blainey, Paul C.

214

Emergent patterns of diversity and dynamics in natural populations of planktonic Vibrio bacteria  

E-Print Network [OSTI]

Despite the importance of microorganisms for global and engineering processes, currently lacking is a theoretical framework to describe how the structure of a microbial assemblage translates an environmental condition into ...

Thompson, Janelle Renée, 1976-

2005-01-01T23:59:59.000Z

215

Interactions between nitrifying bacteria and hydrocarbon-degrading bacteria during detoxification of oil sands process affected water  

SciTech Connect (OSTI)

Large quantities of process water are produced during the extraction of bitumen from oil sands by the Syncrude and Suncor operations in northern Alberta. Freshly produced tailings water is acutely toxic, but it has been shown to slowly detoxify over time. As detoxification proceeds, there is also a precipitous decrease in ammonia concentrations. The present study examines these two microbially-mediated processes in relation to levels of bacteria and toxicants in mixtures of fresh and aged (detoxified) tailings water. Detoxification of tailings water was greatly accelerated when equal volumes of fresh and detoxified (natural aging for one year) tailings water were mixed. Addition of phosphorus further stimulated detoxification, causing levels of ammonia and naphthenic acids (toxic organic acids leached during bitumen extraction) to decrease to those of detoxified water within two months. Such changes were not observed when phosphorus was not added, or when it was added to less diluted (10-.1 or 3-.1) fresh tailings water. Populations of nitrifying bacteria and naphthenic acid degraders increased markedly in the phosphorus-amended mixtures, but not in its absence. Addition of CS{sub 2} (a specific inhibitor of nitrification) to these mixtures prevented ammonia oxidation. Surprisingly, it also prevented the increase in naphthenic acid-degraders and retarded the loss of naphthenic acids. These results suggest the existence of interactions in fresh tailings water between nitrifying bacteria, naphthenic acid degraders and toxicants. The activity of naphthenic acid-degraders apparently remains low until ammonia is oxidized, whereas that of nitrifying bacteria remains low until concentrations of naphthenic acids or other toxicants decrease below some threshold level. Understanding these interactions may lead to more efficient and effective processes to detoxify oil sands process water.

Sobolewski, A. [Microbial Technologies, Vancouver, British Columbia (Canada); MacKinnon, M. [Syncrude Research, Edmonton, Alberta (Canada)

1995-12-31T23:59:59.000Z

216

Fuel from Bacteria, CO2, Water, and Solar Energy: Engineering a Bacterial Reverse Fuel Cell  

SciTech Connect (OSTI)

Electrofuels Project: Harvard is engineering a self-contained, scalable Electrofuels production system that can directly generate liquid fuels from bacteria, carbon dioxide (CO2), water, and sunlight. Harvard is genetically engineering bacteria called Shewanella, so the bacteria can sit directly on electrical conductors and absorb electrical current. This current, which is powered by solar panels, gives the bacteria the energy they need to process CO2 into liquid fuels. The Harvard team pumps this CO2 into the system, in addition to water and other nutrients needed to grow the bacteria. Harvard is also engineering the bacteria to produce fuel molecules that have properties similar to gasoline or diesel fuel—making them easier to incorporate into the existing fuel infrastructure. These molecules are designed to spontaneously separate from the water-based culture that the bacteria live in and to be used directly as fuel without further chemical processing once they’re pumped out of the tank.

None

2010-07-01T23:59:59.000Z

217

Engineering Bacteria for Efficient Fuel Production: Novel Biological Conversion of Hydrogen and Carbon Dioxide Directly into Free Fatty Acids  

SciTech Connect (OSTI)

Electrofuels Project: OPX Biotechnologies is engineering a microorganism currently used in industrial biotechnology to directly produce a liquid fuel from hydrogen and carbon dioxide (CO2). The microorganism has the natural ability to use hydrogen and CO2 for growth. OPX Biotechnologies is modifying the microorganism to divert energy and carbon away from growth and towards the production of liquid fuels in larger, commercially viable quantities. The microbial system will produce a fuel precursor that can be chemically upgraded to various hydrocarbon fuels.

None

2010-07-12T23:59:59.000Z

218

Dynamic Modeling of Aerobic Growth of Shewanella oneidensis. Predicting Triauxic Growth, Flux Distributions and Energy Requirement for Growth  

SciTech Connect (OSTI)

A model-based analysis is conducted to investigate metabolism of Shewanella oneidensis MR-1 strain in aerobic batch culture, which exhibits an intriguing growth pattern by sequentially consuming substrate (i.e., lactate) and by-products (i.e., pyruvate and acetate). A general protocol is presented for developing a detailed network-based dynamic model for S. oneidensis based on the Lumped Hybrid Cybernetic Model (LHCM) framework. The L-HCM, although developed from only limited data, is shown to accurately reproduce exacting dynamic metabolic shifts, and provide reasonable estimates of energy requirement for growth. Flux distributions in S. oneidensis predicted by the L-HCM compare very favorably with 13C-metabolic flux analysis results reported in the literature. Predictive accuracy is enhanced by incorporating measurements of only a few intracellular fluxes, in addition to extracellular metabolites. The L-HCM developed here for S. oneidensis is consequently a promising tool for the analysis of intracellular flux distribution and metabolic engineering.

Song, Hyun-Seob; Ramkrishna, Doraiswami; Pinchuk, Grigoriy E.; Beliaev, Alex S.; Konopka, Allan; Fredrickson, Jim K.

2013-01-01T23:59:59.000Z

219

Removal of Heavy Metals from Industrial Effluent Using Bacteria  

E-Print Network [OSTI]

Industrial development results in the generation of industrial effluents, and if untreated results in water, sediment and soil pollution. (Fakayode and Onianwa, 2002 ? Fakayode, 2005). Industrial wastes and emission contain toxic and hazardous substances, most of which are detrimental to human health (Jimena et al.,2008 ? Ogunfowokan et al.,2005 ? Rajaram et al.,2008). The key pollutants include heavy metals, chemical wastes and oil spills etc. Heavy metal resistant bacteria have significant role in bioremediation of heavy metals in wastewater. The objective of this work is to study the role of bacteria in removing the heavy metals present in the industrial effluent.Five effluent samples out of nine were selected for this study due to high content of heavy metals. The heavy metals Hg and Cu were removed by Bacillus sp. The average Hg reduction was 45 % and Cu reduction was recorded as 62%. The heavy metals Cd, As and Co were removed by Pseudomonas sp. The average Cd reduction was 56%, average As reduction was 34 % and average Co reduction was recorded as 53%. The heavy metals Cd and Cu were removed by Staphylococcus sp. The average Cd reduction was 44 % and average Cu reduction was recorded as 34 %.

Manisha N; Dinesh Sharma; Arun Kumar

220

Process for generation of hydrogen gas from various feedstocks using thermophilic bacteria  

DOE Patents [OSTI]

A method for producing hydrogen gas is provided comprising selecting a bacteria from the Order Thermotogales, subjecting the bacteria to a feedstock and to a suitable growth environment having an oxygen concentration below the oxygen concentration of water in equilibrium with air; and maintaining the environment at a predetermined pH and at a temperature of at least approximately 45.degree. C. for a time sufficient to allow the bacteria to metabolize the feedstock.

Ooteghem, Suellen Van (Morgantown, WV)

2005-09-13T23:59:59.000Z

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

Process for Generation of Hydrogen Gas from Various Feedstocks Using Thermophilic Bacteria  

SciTech Connect (OSTI)

A method for producing hydrogen gas is provided comprising selecting a bacteria from the Order Thermotogales, subjecting the bacteria to a feedstock and to a suitable growth environment having an oxygen concentration below the oxygen concentration of water in equilibrium with air; and maintaining the environment at a predetermined pH and at a temperature of at least approximately 45 degrees C. for a time sufficient to allow the bacteria to metabolize the feedstock.

Ooteghem Van, Suellen

2005-09-13T23:59:59.000Z

222

E-Print Network 3.0 - atypical bacteria accompanying Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Swine Flu and Common Infections to Prepare For Summary: Other common causes - Whooping cough (pertussis) - Atypical bacteria Symptoms - Severe cough - Runny... :...

223

Contribution of Iron-Reducing Bacteria to Mercury Methylation in Marine Sediments  

E-Print Network [OSTI]

in continental margin sediments off central Chile. Limnologyof microbial iron reduction in sediments of the Baltic-Northreducing bacteria from sediments of an acid stressed lake.

Fleming, Emily J.; Nelson, D C

2006-01-01T23:59:59.000Z

224

Purple Bacteria Develops Its Own Form of Sunscreen | U.S. DOE...  

Office of Science (SC) Website

Yellowstone National Park, is due to carotenoid molecules, produced by huge mats of algae and bacteria (Photo from http:en.wikipedia.orgwikiCarotenoid External link )...

225

E-Print Network 3.0 - animal-associated bacteria warfare Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

pheromones by bacteria in the female's gut. Attraction pheromones dissipate from Source: Colorado at Boulder, University of - CIRES, Center for Science and Technology Policy...

226

E-Print Network 3.0 - acidophilic bioleaching-associated bacteria...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

for: acidophilic bioleaching-associated bacteria Page: << < 1 2 3 4 5 > >> 1 Biodiversity II: Kingdoms Eubacteria and Archaebacteria Summary: 1 Biodiversity II: Kingdoms...

227

E-Print Network 3.0 - acidophilic heterotrophic bacteria Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

search results for: acidophilic heterotrophic bacteria Page: << < 1 2 3 4 5 > >> 1 Biodiversity II: Kingdoms Eubacteria and Archaebacteria Summary: beneficial genes Bacterial...

228

E-Print Network 3.0 - acidophilic bacteria kosansei Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

search results for: acidophilic bacteria kosansei Page: << < 1 2 3 4 5 > >> 1 Biodiversity II: Kingdoms Eubacteria and Archaebacteria Summary: 1 Biodiversity II: Kingdoms...

229

E-Print Network 3.0 - anaerobic bacteria regulation Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

regu-lationcontributestothetranscriptionalregula- Summary: , 35043 Marburg, Germany. E-mail: thauer@ mpi-marburg.mpg.de Gram-positive bacteria 3 In strict...

230

E-Print Network 3.0 - anaerobic ammonium-oxidizing bacteria Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

organic waste, converting... of anaerobic bacteria, these compounds decompose to yield a biogas that comprises of methane (CH.) and carbon... not processed anaerobically. The...

231

E-Print Network 3.0 - anaerobic bacteria thermoanaerobacter Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

organic waste, converting... of anaerobic bacteria, these compounds decompose to yield a biogas that comprises of methane (CH.) and carbon... not processed anaerobically. The...

232

E-Print Network 3.0 - anaerobic selenate-respiring bacteria Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

organic waste, converting... of anaerobic bacteria, these compounds decompose to yield a biogas that comprises of methane (CH.) and carbon... not processed anaerobically. The...

233

E-Print Network 3.0 - anaerobic bacteria isolated Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

(White) isolate. The signicance of bacteria for development... by Pearson cor- relation test (Minitab 1997). Results Isolation ... Source: Schal, Coby - Department of Entomology,...

234

E-Print Network 3.0 - abundant culturable bacteria Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Sample search results for: abundant culturable bacteria Page: << < 1 2 3 4 5 > >> 1 ria and Virus Like Particles (VLPs) abundance were monitored in enriched seawater batch...

235

E-Print Network 3.0 - agricultural soil bacteria Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

ies from... for reducing bacteria runoff. With almost 400 water bodies impaired by bacte- ria, a variety of resources... is providing a coordinated and comprehen- sive education...

236

E-Print Network 3.0 - acid bacteria inhibiting Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

while the activity of isolate C0593... those concentrations that enhance or inhibit the test bacteria or consortium. Conclusions The use... .V. All rights reserved 0167 - 7012...

237

E-Print Network 3.0 - anaerobic sulfate-reducing bacteria Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

USA EVGENYA S. SHELOBOLINA Summary: .J.P. and Woodward, J.C. (1993) Enzymatic iron and uranium reduction by sulfate- reducing bacteria. Marine Geology... . These results suggest...

238

Detergent composition comprising a cellulase containing cell-free fermentate produced from microorganism ATCC 55702 or mutant thereof  

DOE Patents [OSTI]

Bacteria which produce large amounts of a cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

Dees, H.C.

1998-07-14T23:59:59.000Z

239

Detergent composition comprising a cellulase containing cell-free fermentate produced from microorganism ATCC 55702 or mutant thereof  

DOE Patents [OSTI]

Bacteria which produce large amounts of a cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

Dees, H. Craig (Lenoir City, TN)

1998-01-01T23:59:59.000Z

240

Nitrogen availability and indirect measurements of greenhouse gas emissions from aerobic and anaerobic biowaste digestates applied to agricultural soils  

SciTech Connect (OSTI)

Highlights: • Nitrogen release in digestate-amended soil depends on the digestate type. • Overall N release is modulated by digestate mineral and mineralisable N contents. • Microbial immobilisation does not influence overall release of digestate N in soil. • Digestate physical properties and soil type interact to affect overall N recovery. • High labile C inputs in digestate may promote denitrification in fine-textured soil. - Abstract: Recycling biowaste digestates on agricultural land diverts biodegradable waste from landfill disposal and represents a sustainable source of nutrients and organic matter (OM) to improve soil for crop production. However, the dynamics of nitrogen (N) release from these organic N sources must be determined to optimise their fertiliser value and management. This laboratory incubation experiment examined the effects of digestate type (aerobic and anaerobic), waste type (industrial, agricultural and municipal solid waste or sewage sludge) and soil type (sandy loam, sandy silt loam and silty clay) on N availability in digestate-amended soils and also quantified the extent and significance of the immobilisation of N within the soil microbial biomass, as a possible regulatory mechanism of N release. The digestate types examined included: dewatered, anaerobically digested biosolids (DMAD); dewatered, anaerobic mesophilic digestate from the organic fraction of municipal solid waste (DMADMSW); liquid, anaerobic co-digestate of food and animal slurry (LcoMAD) and liquid, thermophilic aerobic digestate of food waste (LTAD). Ammonium chloride (NH{sub 4}Cl) was included as a reference treatment for mineral N. After 48 days, the final, maximum net recoveries of mineral N relative to the total N (TN) addition in the different digestates and unamended control treatments were in the decreasing order: LcoMAD, 68%; LTAD, 37%, DMAD, 20%; and DMADMSW, 11%. A transient increase in microbial biomass N (MBN) was observed with LTAD application, indicating greater microbial activity in amended soil and reflecting the lower stability of this OM source, compared to the other, anaerobic digestate types, which showed no consistent effects on MBN compared to the control. Thus, the overall net release of digestate N in different soil types was not regulated by N transfer into the soil microbial biomass, but was determined primarily by digestate properties and the capacity of the soil type to process and turnover digestate N. In contrast to the sandy soil types, where nitrate (NO{sub 3}{sup -}) concentrations increased during incubation, there was an absence of NO{sub 3}{sup -} accumulation in the silty clay soil amended with LTAD and DMADMSW. This provided indirect evidence for denitrification activity and the gaseous loss of N, and the associated increased risk of greenhouse gas emissions under certain conditions of labile C supply and/or digestate physical structure in fine-textured soil types. The significance and influence of the interaction between soil type and digestate stability and physical properties on denitrification processes in digestate-amended soils require urgent investigation to ensure management practices are appropriate to minimise greenhouse gas emissions from land applied biowastes.

Rigby, H.; Smith, S.R., E-mail: s.r.smith@imperial.ac.uk

2013-12-15T23:59:59.000Z

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


241

Coliphages and bacteria in ground water from Tehran, Iran  

SciTech Connect (OSTI)

The purpose of this study was to examine the microbial quality of Tehran's ground water and selected springs, using coliphages and selected bacteria as indicator organisms. The water table in Tehran varies from approximately 160 meters in the north to approximately 5 meters in the south. Individual wells and subterranean man-made aqueducts (qanate) tap the ground water. Since Tehran lacks municipal sewage facilities, waste disposal is by means of seepage pits, privies and leaching cesspools. There is potential for waste from these sites to leach into the ground water, particularly in the south where the water table is near the surface and the clay content of the soil holds moisture during periods of heavy rainfall.

Shariatpanahi, M.; Anderson, A.C.

1987-07-01T23:59:59.000Z

242

Algal Research1 Screening and selection of growth-promoting bacteria for Dunaliella cultures2  

E-Print Network [OSTI]

and potential applications for microalgae, which include food,44 animal feed, healthcare, energy microalgae species and identified using 16S rRNA phylogenetic analysis. The recovered bacteria were27 found38 accumulation in Dunaliella sp. cells.39 Keywords: Microalgae, Dunaliella, bacteria, interaction

Paris-Sud XI, Université de

243

A simple bioclogging model that accounts for spatial spreading of bacteria  

E-Print Network [OSTI]

is presented that accounts for spatial expansion of the bacterial popu- lation in the soil. The bacteria move: 35K65, 35M10, 68U20, 76S05, 92D25 1 Introduction In soils, aquifers, and many other porous harmful environ- mental factors and mechanical washout. Naturally occurring bacteria are a major

Demaret, Laurent

244

Nano scale devices for plasmonic nanolithography and rapid sensing of bacteria  

E-Print Network [OSTI]

NANO SCALE DEVICES FOR PLASMONIC NANOLITHOGRAPHY AND RAPID SENSING OF BACTERIA A Dissertation by SUNGKYU SEO Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements... for the degree of DOCTOR OF PHILOSOPHY December 2007 Major Subject: Electrical Engineering NANO SCALE DEVICES FOR PLASMONIC NANOLITHOGRAPHY AND RAPID SENSING OF BACTERIA A Dissertation by SUNGKYU SEO...

Seo, Sungkyu

2008-10-10T23:59:59.000Z

245

Clay enhancement of methane, low molecular weight hydrocarbon and halocarbon conversion by methanotrophic bacteria  

DOE Patents [OSTI]

An apparatus and method for increasing the rate of oxidation of toxic vapors by methanotrophic bacteria. The toxic vapors of interest are methane and trichloroethylene. The apparatus includes a gas phase bioreactor within a closed loop pumping system or a single pass system. The methanotrophic bacteria include Methylomonas methanica, Methylosinus trichosporium, and uncharacterized environmental enrichments.

Apel, William A. (Idaho Falls, ID); Dugan, Patrick R. (Idaho Falls, ID)

1995-01-01T23:59:59.000Z

246

Impact of carbon nanotubes on the ingestion and digestion of bacteria by  

E-Print Network [OSTI]

Impact of carbon nanotubes on the ingestion and digestion of bacteria by ciliated protozoa PARNIAN,4 by ecotoxicologists because of its role in the regulation of microbial populations through the ingestion and digestion to aggregate, which impedes their ability to ingest and digest their prey bacteria species, although it might

Le Roy, Robert J.

247

Novel Methane, Ethane, and Propane Oxidizing Bacteria at Marine Hydrocarbon Seeps Identified by Stable Isotope Probing  

E-Print Network [OSTI]

Novel Methane, Ethane, and Propane Oxidizing Bacteria at Marine Hydrocarbon Seeps Identified by Stable Isotope Probing Running Title: Novel Methane, Ethane, and Propane Oxidizing Bacteria Section incubating sediment with 13 C-labeled methane, ethane, or propane, we5 confirmed the incorporation of 13 C

Sessions, Alex L.

248

Chromatic acclimation and population dynamics of green sulfur bacteria grown with spectrally tailored light  

E-Print Network [OSTI]

Living organisms have to adjust to their surrounding in order to survive in stressful conditions. We study this mechanism in one of most primitive creatures - photosynthetic green sulfur bacteria. These bacteria absorb photons very efficiently using the chlorosome antenna complexes and perform photosynthesis in extreme low-light environments. How the chlorosomes in green sulfur bacteria are acclimated to the stressful light conditions, for instance, if the spectrum of light is not optimal for absorption, is unknown. Studying Chlorobaculum tepidum cultures with far-red to near-infrared light-emitting diodes, we found that these bacteria react to changes in energy flow by regulating the amount of light-absorbing pigments and the size of the chlorosomes. Surprisingly, our results indicate that the bacteria can survive in near-infrared lights capturing low-frequency photons by the intermediate units of the light-harvesting complex. The latter strategy may be used by the species recently found near hydrothermal ve...

Saikin, Semion K; Huh, Joonsuk; Hannout, Moataz; Wang, Yaya; Zare, Farrokh; Aspuru-Guzik, Alan; Tang, Joseph Kuo-Hsiang

2014-01-01T23:59:59.000Z

249

Dynamic estimation of specific growth rates and concentrations of bacteria for the anaerobic digestion  

E-Print Network [OSTI]

by microorganisms into biogas (methane and carbon dioxide) and digestate (natural manure) in the absence of oxygen digestion S. Diop1 and I. Simeonov2 Abstract-- The paper proposes an observability anal- ysis and estimation measured quantities ­ the dilution rate and the flow rates of methane and carbon dioxide in the biogas

Boyer, Edmond

250

Final technical report for project titled Quantitative Characterization of Cell Aggregation/Adhesion as Predictor for Distribution and Transport of Microorganisms in Subsurface Environment  

SciTech Connect (OSTI)

This project aims to explore and develop enabling methodology and techniques for nano-scale characterization of microbe cell surface contact mechanics, interactions and adhesion quantities that allow for identification and quantification of indicative properties related to microorganism migration and transport behavior in porous media and in subsurface environments. Microbe transport has wide impact and therefore is of great interest in various environmental applications such as in situ or enhanced subsurface bioremediation,filtration processes for water and wastewater treatments and protection of drinking water supplies. Although great progress has been made towards understanding the identities and activities of these microorganisms in the subsurface, to date, little is known of the mechanisms that govern the mobility and transport of microorganisms in DOE’s contaminated sites, making the outcomes of in situ natural attenuation or contaminant stability enhancement unpredictable. Conventionally, movement of microorganisms was believed to follows the rules governing solute (particle) transport. However, recent studies revealed that cell surface properties, especially those pertaining to cell attachment/adhesion and aggregation behavior, can cause the microbe behavior to deviate from non-viable particles and hence greatly influence the mobility and distribution of microorganisms in porous media.This complexity highlights the need to obtain detailed information of cell-cell and cell-surface interactions in order to improve and refine the conceptual and quantitative model development for fate and transport of microorganisms and contaminant in subsurface. Traditional cell surface characterization methods are not sufficient to fully predict the deposition rates and transport behaviors of microorganism observed. A breakthrough of methodology that would allow for quantitative and molecular-level description of intrinsic cell surface properties indicative for cell-surface interactions is essential for the field. To tackle this, we have developed a number of new Bio-nanomechanical techniques, including reflection interference contrast microscopy (RICM) and bio-AFM (Atomic Force Microscopy), for cell adhesion-detachment measurement of the long-range surface interactions, in combination with mathematical modeling, which would allow us to characterize the mechanical behavior from single cell to multi-cell aggregate, critical thresholds for large scale coaggregation and transportation of cells and aggregates in the presence of long range inter-surface forces etc. Although some technical and mathematical challenges remain, the preliminary results promise great breakthrough potential. In this study, we investigated the cellular surface characteristics of representative bio-remediating microorganisms relevant to DOE IFRC (Integrated Field-Scale Subsurface Research Challenges) sites and their transport behaviors in porous media, aiming to draw a groundbreaking correlation between the micro-scale genetic and biological origin-based cell surface properties, the consequent mechanical adhesion and aggregation behaviors, and the macro-scale microbial mobility and retention in porous media, which are unavailable in the literature. The long-term goal is to significantly improve the mechanistic and quantitative understanding of microbial mobility, sorption, and transport within reactive transport models as needed to manipulate subsurface contaminant fate and transport predictions.

Gu, April Z [Northeastern University; Wan, Kai-tak [Northeastern Univeristy

2014-09-02T23:59:59.000Z

251

Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips  

DOE Patents [OSTI]

The present invention relates to methods and compositions for using nucleotide sequence variations of 16S and 23S rRNA within the B. cereus group to discriminate a highly infectious bacterium B. anthracis from closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations and discriminate B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed samples, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

Bavykin, Sergei G. (Darien, IL); Mirzabekova, legal representative, Natalia V. (Westmont, IL); Mirzabekov, deceased, Andrei D. (Westmont, IL)

2007-12-04T23:59:59.000Z

252

Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips  

DOE Patents [OSTI]

The present invention is directed to a novel method of discriminating a highly infectious bacterium Bacillus anthracis from a group of closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations. The identification and analysis of these sequence variations enables positive discrimination of isolates of the B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed probes, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

Bavykin, Sergei G. (Darien, IL); Mirzabekov, Andrei D. (Moscow, RU)

2007-10-30T23:59:59.000Z

253

Genome-Assisted Analysis of Dissimilatory Metal-Reducing Bacteria  

SciTech Connect (OSTI)

Whole genome sequence for Shewanella oneidensis and Geobacter sulfurreducens has provided numerous new biological insights into the function of these model dissimilatory metal-reducing bacteria. Many of the discoveries, including the identification of a high number of c-type cytochromes in both organisms, have been the result of comparative genomic analyses including several that were experimentally confirmed. Genome sequence has also aided the identification of genes important for the reduction of metal ions and other electron acceptors utilized by these organisms during anaerobic growth by facilitating the identification of genes disrupted by random insertions. Technologies for assaying global expression patterns for genes (mRNA) and proteins have also been enabled by the availability of genome sequence but their application has been limited mainly to the analysis of the role of global regulatory genes and to identifying genes expressed or repressed in response to specific electron acceptors. It is anticipated that details regarding the mechanisms of metal ion respiration, and metabolism in general, will eventually be revealed by comprehensive, systems-level analyses enabled by functional genomic analyses.

Fredrickson, Jim K.; Romine, Margaret F.

2005-06-01T23:59:59.000Z

254

Ribulose-1,5-bisphosphate carboxylase/oxygenase gene expression and diversity of Lake Erie planktonic microorganisms  

SciTech Connect (OSTI)

Carbon dioxide fixation is carried out primarily through the Calvin-Benson-Bassham reductive pentose phosphate cycle, in which rubulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) is the key enzyme. The primary structure of the large subunit of form I RubisCO is well conserved; however, four distinct types, A, B, C, and D, may be distinguished. To better understand the environmental regulation of RubisCO in Lake Erie phytoplanktonic microorganisms, we have isolated total RNA and DNA from four Lake Erie sampling sites. Probes prepared from RubisCO large-subunit genes (rbcL) of the freshwater cyanobacterium Synechococcus sp. strain PCC6301 (representative of type IB) and the diatom Cylindrotheca sp. strain N1 (representative of type ID) was determined. It appeared that type ID (diatom) rbcL gene expression per gene dose decreased as the sampling sites shifted toward open water. By contrast, a similar trend was not observed for cyanobacterial (type IB) rbcL gene expression per gene dose. Thus far, a total of 21 clones of rbcL genes derived from mRNA have been obtained and completely sequenced from the Ballast Island site. For surface water samples, deduced amino acid sequences of five of six clones appeared to be representative of green algae. In contrast, six of nine sequenced rbcL clones from 10-m-deep samples were a chromophytic and rhodophytic lineages. At 5 m deep, the active CO{sub 2}-fixing planktonic organisms represented a diverse group, including organisms related to Chlorella ellipsoidea, Cylindrotheca sp. strain N1, and Olisthodiscus luteus. Although many more samplings at diverse sites must be accomplished, the discovery of distinctly different sequences of rbcL mRNA at different water depths suggests that there is a stratification of active CO{sub 2}-fixing organisms in western Lake Erie. 54 refs., 7 figs.

Xu, H.H.; Tabita, F.R. [Ohio State Univ., Columbus, OH (United States)

1996-06-01T23:59:59.000Z

255

Radiation-resistant microorganism  

DOE Patents [OSTI]

An isolated and purified bacterium is provided which was isolated from a high-level radioactive waste site of mixed waste. The isolate has the ability to degrade a wide variety of organic contaminants while demonstrating high tolerance to ionizing radiation. The organism is uniquely suited to bioremediation of a variety or organic contaminants while in the presence of ionizing radiation.

Fliermans, Carl B.

2010-06-15T23:59:59.000Z

256

Genetically modified microorganisms for  

E-Print Network [OSTI]

-proteins. Thus far, Shewanella oneidensis and Geobacter sulfurreducens have been extensively studied into electricity in much more efficient ways. This chapter will discuss recent work on the investigation be possible in the near future. 6.2 EXTRACELLULAR RESPIRATION IN SHEWANELLA ONEIDENSIS AND GEOBACTER

Angenent, Lars T.

257

Population Genomics of Early Events in the Ecological Differentiation of Bacteria  

E-Print Network [OSTI]

Genetic exchange is common among bacteria, but its effect on population diversity during ecological differentiation remains controversial. A fundamental question is whether advantageous mutations lead to selection of clonal ...

Shapiro, B. J.

258

E-Print Network 3.0 - acid bacteria elicit Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Zosteric Acid and Their Potential Application as Summary: . putida and Lake Erie bacteria, respectively. Zosteric acid EC50 values were 167 6 3.9 and 375 6 10 mg... )....

259

E-Print Network 3.0 - acid bacteria revisited Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Zosteric Acid and Their Potential Application as Summary: . putida and Lake Erie bacteria, respectively. Zosteric acid EC50 values were 167 6 3.9 and 375 6 10 mg... )....

260

E-Print Network 3.0 - acid bacteria structure Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Zosteric Acid and Their Potential Application as Summary: . putida and Lake Erie bacteria, respectively. Zosteric acid EC50 values were 167 6 3.9 and 375 6 10 mg... )....

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

E-Print Network 3.0 - acid bacteria affect Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Zosteric Acid and Their Potential Application as Summary: . putida and Lake Erie bacteria, respectively. Zosteric acid EC50 values were 167 6 3.9 and 375 6 10 mg... )....

262

Amoebae/bacteria consortia and uses for degrading wastes and contaminants  

DOE Patents [OSTI]

A method of altering trinitrotoluene includes the steps of: providing an amoeba/bacteria consortium, particularly ATCC 40908 or a mutant thereof possessing all the identifying characteristics thereof; and contacting the consortium with trinitrotoluene to alter the trinitrotoluene.

Tyndall, Richard L. (Clinton, TN)

1996-01-01T23:59:59.000Z

263

Caenorhabditis elegans NPR-1–mediated behaviors are suppressed in the presence of mucoid bacteria  

E-Print Network [OSTI]

Caenorhabditis elegans exhibits a diverse range of behaviors in response to bacteria. The presence of bacterial food influences C. elegans aerotaxis, aggregation, locomotion, and pathogen avoidance behaviors through the ...

Reddy, Kirthi C.

264

Engineering Bacteria for Production of Rhamnolipid as an Agent for Enhanced  

E-Print Network [OSTI]

ARTICLE Engineering Bacteria for Production of Rhamnolipid as an Agent for Enhanced Oil Recovery applications, including enhanced oil recovery (EOR), biodegradation, and bio- remediation. Rhamnolipid; transposome; chromosomal insertion; interfacial tension; enhanced oil recovery Introduction Surfactants

Goddard III, William A.

265

Nano scale devices for plasmonic nanolithography and rapid sensing of bacteria  

E-Print Network [OSTI]

This dissertation contains two different research topics. One is a ‘Nano Scale Device for Plasmonic Nanolithography – Optical Antenna’ and the other is a ‘Nano Scale Device for Rapid Sensing of Bacteria – SEPTIC’. Since these two different research...

Seo, Sungkyu

2009-05-15T23:59:59.000Z

266

The battle of bacteria: Agencies, stakeholders focusing on restoring water quality  

E-Print Network [OSTI]

to watershed stakeholders, who will determine the next steps in managing water quality in the tributaries. The TMDL task force was also charged with developing a roadmap for scientific research on how bacteria behave under different conditions. Tailored...

Foust, Margaret

2011-01-01T23:59:59.000Z

267

The battle of bacteria: Agencies, stakeholders focusing on restoring water quality  

E-Print Network [OSTI]

to watershed stakeholders, who will determine the next steps in managing water quality in the tributaries. The TMDL task force was also charged with developing a roadmap for scientific research on how bacteria behave under different conditions. Tailored...

Foust, Margaret

2010-01-01T23:59:59.000Z

268

Biotemplated Synthesis of Gold NanoparticleBacteria Cellulose Nanofiber Nanocomposites and Their  

E-Print Network [OSTI]

produced by acetic acid bacteria Acetobacter xylinum using D-glucose as the carbon source, which usually of sisal fibers by in situ fermentation and obtained a new class of completely renewable and biodegradable

Qi, Limin

269

Cargo delivery into gram-negative bacteria via enterobactin uptake machinery  

E-Print Network [OSTI]

Chapter 1. Introduction to Iron Homeostasis and Siderophores Iron is an essential nutrient for almost all living organisms. This Chapter presents an overview of iron homeostasis in human and bacteria, as well as the biology ...

Zheng, Tengfei

2014-01-01T23:59:59.000Z

270

Bacteria Harnessing Complexity Eshel Ben Jacob, Yakir Aharonov and Yoash Shapira  

E-Print Network [OSTI]

1 Bacteria Harnessing Complexity By Eshel Ben Jacob, Yakir Aharonov and Yoash Shapira School, exchange of meaning-bearing chemical messages, and dialogues. The meaning-based communication permits

Jacob, Eshel Ben

271

The incidence and significance of anaerobic bacteria in the equine uterus  

E-Print Network [OSTI]

that anaerobic bacteria may participate in inflammatory processes of the equine uterus (Kenney 1978a, Reynolds 1978, Ricketts 1981, Brook 1984). Elliott et al. (1971) conducted a study to obtain uterine cultures under anaerobic conditions but failed to obtain... not definitively been proven whether the normal equine uterine environment is sterile (Peterson et al. 1969, Hughes 1978) or if bacteria may normally be present in the non-inflamed uterus (Farrelly and Mullaney 1964, Kenney 1978a, Samuel et al. 1979). Knudsen...

Bolinger, Dean Roger

2012-06-07T23:59:59.000Z

272

Effects of UV Light Disinfection on Tetracycline Resistant Bacteria in Wastewater Effluents  

E-Print Network [OSTI]

EFFECTS OF UV LIGHT DISINFECTION ON TETRACYCLINE RESISTANT BACTERIA IN WASTEWATER EFFLUENTS A Thesis by HANNAH CHILDRESS Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment... of the requirements for the degree of MASTER OF SCIENCE August 2010 Major Subject: Biological and Agricultural Engineering EFFECTS OF UV LIGHT DISINFECTION ON TETRACYCLINE RESISTANT BACTERIA IN WASTEWATER EFFLUENTS A Thesis by HANNAH...

Childress, Hannah

2011-10-21T23:59:59.000Z

273

Identification of Bacteria in Biofilm and Bulk Water Samples from a Nonchlorinated Model Drinking Water Distribution System: Detection of a Large Nitrite-Oxidizing Population Associated with Nitrospira spp.  

E-Print Network [OSTI]

Identification of Bacteria in Biofilm and Bulk Water SamplesNo. 12 Identification of Bacteria in Biofilm and Bulk Water

Martiny, A. C; Albrechtsen, H.-J.; Arvin, E.; Molin, S.

2005-01-01T23:59:59.000Z

274

The effect of polyacrylamide polymers and formaldehyde on selected strains of oilfield related bacteria  

SciTech Connect (OSTI)

A preliminary study of the effect of two polyacrylamide polymers and formaldehyde on certain strains of oil field related bacteria was performed. Since at this stage of the development of planning the proposed North Sea polymer flood field samples of native bacteria populations and fluid samples were not available, the preliminary study was made using pure strains of bacteria associated with the oilfield environment. The tests were run using ideal growth conditions of temperature and culture media. The purpose of the study was to determine the effect of proposed polyacrylamide polymer flooding materials on the growth of selected strains of bacteria belonging to families common to the oilfield environment. The effect of 100 ppm of formaldehyde on these organisms also was investigated. The results of the tests showed that these bacteria responded differently to exposure to formaldehyde, polyacrylamide polymers and mixtures of both. These results indicate another possible mechanism for the occurrence of high bacteria related corrosion rates reported to occur on the producing side of polymer floods.

Farquhar, G.B. [Texaco E.P.T.D., Houston, TX (United States)

1996-08-01T23:59:59.000Z

275

L\\'evy Fluctuations and Tracer Diffusion in Dilute Suspensions of Algae and Bacteria  

E-Print Network [OSTI]

Swimming microorganisms rely on effective mixing strategies to achieve efficient nutrient influx. Recent experiments, probing the mixing capability of unicellular biflagellates, revealed that passive tracer particles exhibit anomalous non-Gaussian diffusion when immersed in a dilute suspension of self-motile Chlamydomonas reinhardtii algae. Qualitatively, this observation can be explained by the fact that the algae induce a fluid flow that may occasionally accelerate the colloidal tracers to relatively large velocities. A satisfactory quantitative theory of enhanced mixing in dilute active suspensions, however, is lacking at present. In particular, it is unclear how non-Gaussian signatures in the tracers' position distribution are linked to the self-propulsion mechanism of a microorganism. Here, we develop a systematic theoretical description of anomalous tracer diffusion in active suspensions, based on a simplified tracer-swimmer interaction model that captures the typical distance scaling of a microswimmer'...

Zaid, Irwin M; Yeomans, Julia M

2010-01-01T23:59:59.000Z

276

Development of a Multiplexed, Bead-Based Assessment Tool for Rapid Identification and Quantitation of Microorganisms in Field Samples. Final Report  

SciTech Connect (OSTI)

This was the final report for DOE NABIR grant DE-FG02-01ER63264 (PI Mary Lowe). The grant was entitled ''Development of a Multiplexed Bead-Based Assessment Tool for Rapid Identification and Quantitation of Microorganisms in Field Samples.'' The grant duration was one year. The purpose was to develop a bead-based assay for measuring analyte DNAs in environmental PCR products and to apply the method to a field experiment. The primary experiment was located at the UMTRA Old Rifle site.

Lowe, M.; Halden, R.

2002-10-09T23:59:59.000Z

277

Turning Bacteria into Biofuel: Development of an Integrated Microbial Electrocatalytic (MEC) System for Liquid Biofuel Production from CO2  

SciTech Connect (OSTI)

Electrofuels Project: LBNL is improving the natural ability of a common soil bacteria called Ralstonia eutropha to use hydrogen and carbon dioxide for biofuel production. First, LBNL is genetically modifying the bacteria to produce biofuel at higher concentrations. Then, LBNL is using renewable electricity obtained from solar, wind, or wave power to produce high amounts of hydrogen in the presence of the bacteria—increasing the organism’s access to its energy source and improving the efficiency of the biofuel-creation process. Finally, LBNL is tethering electrocatalysts to the bacteria’s surface which will further accelerate the rate at which the organism creates biofuel. LBNL is also developing a chemical method to transform the biofuel that the bacteria produce into ready-to-use jet fuel.

None

2010-08-01T23:59:59.000Z

278

Interactions of microbes in aquatic systems Uncultured populations of bacteria were analyzed in aquatic systems and populations related  

E-Print Network [OSTI]

and quantitative analysis of Archaea in anaerobic sediments of Lake Rotsee (Lucerne growing by sulfur disproportionation and the purple sulfur bacteria acting as biotic

Upchurch, Gary - Department of Biology, Texas State University

279

The calcification of staphylococcus aureus bacteria by the mineralization by inhibitor exclusion mechanism : a potential defense mechanism against bacterial infections  

E-Print Network [OSTI]

to all life forms and phosphorous is a necessary componentof calcium and phosphorous suggests that calcificationpeaks for carbon and phosphorous in the calcified bacteria

Truong, Linh Y.

2011-01-01T23:59:59.000Z

280

Understanding the Role of the Bacteria, Isolated from the Hanford Site Soil, on the Fate and Transport of Uranium.  

E-Print Network [OSTI]

?? Bacteria are known for their abilities to influence the geochemical processes and affect the mobility of contaminants in the subsurface. Arthrobacter strain G975 was… (more)

Carvajal, Denny A

2011-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

Genomics Encyclopedia of Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB): a resource for microsymbiont genomes (2013 DOE JGI Genomics of Energy and Environment 8th Annual User Meeting)  

SciTech Connect (OSTI)

Wayne Reeve of Murdoch University on "Genomics Encyclopedia of Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB): a resource for microsymbiont genomes" at the 8th Annual Genomics of Energy & Environment Meeting on March 27, 2013 in Walnut Creek, Calif.

Reeve, Wayne [Murdoch University

2013-03-01T23:59:59.000Z

282

ENZYME ACTIVITY PROBE AND GEOCHEMICAL ASSESSMENT FOR POTENTIAL AEROBIC COMETABOLISM OF TRICHLOROETHENE IN GROUNDWATER OF THE NORTHWEST PLUME, PADUCAH GASEOUS DIFFUSION PLANT, KENTUCKY  

SciTech Connect (OSTI)

The overarching objective of the Paducah Gaseous Diffusion Plant (PGDP) enzyme activity probe (EAP) effort is to determine if aerobic cometabolism is contributing to the attenuation of trichloroethene (TCE) and other chlorinated solvents in the contaminated groundwater beneath PGDP. The site-specific objective for the EAP assessment is to identify if key metabolic pathways are present and expressed in the microbial community--namely the pathways that are responsible for degradation of methane and aromatic (e.g. toluene, benzene, phenol) substrates. The enzymes produced to degrade methane and aromatic compounds also break down TCE through a process known as cometabolism. EAPs directly measure if methane and/or aromatic enzyme production pathways are operating and, for the aromatic pathways, provide an estimate of the number of active organisms in the sampled groundwater. This study in the groundwater plumes at PGDP is a major part of a larger scientific effort being conducted by Interstate Technology and Regulatory Council (ITRC), U.S. Department of Energy (DOE) Office of Environmental Management (EM), Savannah River National Laboratory (SRNL), and North Wind Inc. in which EAPs are being applied to contaminated groundwater from diverse hydrogeologic and plume settings throughout the U.S. to help standardize their application as well as their interpretation. While EAP data provide key information to support the site specific objective for PGDP, several additional lines of evidence are being evaluated to increase confidence in the determination of the occurrence of biodegradation and the rate and sustainability of aerobic cometabolism. These complementary efforts include: (1) Examination of plume flowpaths and comparison of TCE behavior to 'conservative' tracers in the plume (e.g., {sup 99}Tc); (2) Evaluation of geochemical conditions throughout the plume; and (3) Evaluation of stable isotopes in the contaminants and their daughter products throughout the plume. If the multiple lines of evidence support the occurrence of cometabolism and the potential for the process to contribute to temporal and spatial attenuation of TCE in PGDP groundwater, then a follow-up enzyme probe microcosm study to better estimate biological degradation rate(s) is warranted.

Looney, B; M. Hope Lee, M; S. K. Hampson, S

2008-06-27T23:59:59.000Z

283

Transformations of 14C lignin cell walls of wheat by a fungus and by bacteria from the rumen  

E-Print Network [OSTI]

Transformations of 14C lignin cell walls of wheat by a fungus and by bacteria from the rumen MA but little is known about the fate of lignins. The aim of this work was to study the transformation of 14C lignins of wheat straw by ruminal bacteria and fungi. Cell walls of wheat straw apical internodes

Paris-Sud XI, Université de

284

ORNL researchers are studying how bacteria found in Yellowstone hot springs  

E-Print Network [OSTI]

deconstruction--a major roadblock to the production of advanced biofuels," Giannone said. --Morgan Mc in the development of a cheaper biofuel production process. (Image: Jennifer Morrell-Falvey/ORNL) SCIENCE ORNL explores proteins in Yellowstone bacteria for biofuel inspiration substrates such as pure cellulose

Pennycook, Steve

285

Molecular Diversity of Legume Root-Nodule Bacteria in Kakadu National Park, Northern Territory, Australia  

E-Print Network [OSTI]

, Australia Be´ne´dicte Lafay¤ *, Jeremy J. Burdon Commonwealth Scientific and Industrial Research Organisation (CSIRO) Plant Industry, Centre for Plant Biodiversity Research, Canberra, Australia Background. Symbiotic relationships between leguminous plants (family Fabaceae) and nodule-forming bacteria in Australia

Boyer, Edmond

286

GROWTH OF A POPULATION OF BACTERIA IN A DYNAMICAL HOSTILE ENVIRONMENT  

E-Print Network [OSTI]

´EGINE MARCHAND Abstract. We study the growth of a population of bacteria in a dynami- cal hostile environment´EGINE MARCHAND · We put an oriented edge from (z1, n1) to (z2, n2) if and only if n2 = n1 + 1 and z2 - z1 1 1

287

ADENOSINE TRIPHOSPHATE (ATP) AND DEOXYRIBONUCLEIC ACID (DNA) CONTENT OF MARINE MICROALGAE AND BACTERIA WITH  

E-Print Network [OSTI]

ADENOSINE TRIPHOSPHATE (ATP) AND DEOXYRIBONUCLEIC ACID (DNA) CONTENT OF MARINE MICROALGAE the relationship between DNA and ATP content of marine bacteria and microalgae. This relationship was used. Laboratory-derived DNA:ATP ratios ranged from 8.5 to 33 (wt:wt) for cultures of marine microalgae, and from

Luther, Douglas S.

288

The Role of Fish as Sources and Vectors of Bacteria and  

E-Print Network [OSTI]

The Role of Fish as Sources and Vectors of Bacteria and Influence of Bat Colonies on Indicator) #12;Acknowledgments Dr. Robin Brinkmeyer ­ Bat colony studies Jenny Wrast ­ Fish and Bat study & sediments #12;But what about fish???? #12;But what about bats???? #12;Outline Fish Study Bat Study #12

289

Bacteria can sprout webs of electrical wiring that transform the soil into a geological  

E-Print Network [OSTI]

cells into a web-like electrical circuit, they report (D. Ntarlagiannis et al. Geophys. Res. Lett. 34, L in La Jolla, California. As the bacteria consume nutrients for energy, they spit out unwanted electrons sand infiltrated with a nutrient compound (lactate), and allowed S. oneidensis to grow in this `fake

Lovley, Derek

290

Microfluidic capture and release of bacteria in a conical nanopore array Peng Guo,ab  

E-Print Network [OSTI]

Microfluidic capture and release of bacteria in a conical nanopore array Peng Guo,ab Eric W. Hall a microfluidic device. As an example, we demonstrate that cyanobacteria can be captured, one bacterium per pore, in a conical nanoporous membrane (CNM) integrated into a microfluidic chip. This study, to our knowledge

Zare, Richard N.

291

Winery waste makes fuel Electricity, bacteria break organics in wastewater into hydrogen gas  

E-Print Network [OSTI]

MSNBC.com Winery waste makes fuel Electricity, bacteria break organics in wastewater into hydrogen method for generating hydrogen fuel from wastewater is now operating at a California winery material in the wastewater into hydrogen gas. There is a lot more energy locked in the wastewater than

292

Dynamic estimation of specific growth rates of bacteria for a wastewater treatment process  

E-Print Network [OSTI]

Dynamic estimation of specific growth rates of bacteria for a wastewater treatment process S. Diop1 for specific growth rates for a wastewater treatment process. A 2-stage model of 6 dynamic states is assumed. Steyer, Dynamic model develop- ment and parameter identification for an anaerobic wastewater treatment

Paris-Sud XI, Université de

293

A dynamic estimation scheme of specific growth rates of bacteria for an anaerobic wastewater treatment process  

E-Print Network [OSTI]

A dynamic estimation scheme of specific growth rates of bacteria for an anaerobic wastewater anal- ysis and estimation schemes for specific growth rates for an anaerobic wastewater treatment the organic and inorganic materials) of municipal or industrial wastewater often needs to be highly reduced

Paris-Sud XI, Université de

294

Methanobactin: a copper binding compound having antibiotic and antioxidant activity isolated from methanotrophic bacteria  

DOE Patents [OSTI]

A means and method for treating bacterial infection, providing antioxidant activity, and chelating copper using a copper binding compound produced by methanotrophic bacteria is described. The compound, known as methanobactin, is the first of a new class of antibiotics having gram-positive activity. Methanobactin has been sequenced, and its structural formula determined.

DiSpirito, Alan A. (Ames, IA); Zahn, James A. (Harbor Beach, MI); Graham, David W. (Lawrence, KS); Kim, Hyung J. (St. Paul, MN); Alterman, Michail (Lawrence, KS); Larive, Cynthia (Lawrence, KS)

2007-04-03T23:59:59.000Z

295

Methylation of Mercury by Bacteria Exposed to Dissolved, Nanoparticulate, and Microparticulate Mercuric Sulfides  

E-Print Network [OSTI]

Methylation of Mercury by Bacteria Exposed to Dissolved, Nanoparticulate, and Microparticulate in the environment is partly controlled by the bioavailability of inorganic divalent mercury (Hg(II)) to anaerobic matter to form chemical species that include organic-coated mercury sulfide nanoparticles as reaction

296

Structure-based Inhibitor Discovery against Adenylyl Cyclase Toxins from Pathogenic Bacteria That Cause Anthrax and  

E-Print Network [OSTI]

That Cause Anthrax and Whooping Cough* Received for publication, February 4, 2003, and in revised form, March bacteria that cause anthrax and whooping cough, respectively. Using the structure of the catalytic site pathogenesis and to fight against anthrax and whooping cough. The 2001 anthrax attacks in the United States

Mrksich, Milan

297

Lévy Fluctuations and Tracer Diffusion in Dilute Suspensions of Algae and Bacteria  

E-Print Network [OSTI]

Swimming microorganisms rely on effective mixing strategies to achieve efficient nutrient influx. Recent experiments, probing the mixing capability of unicellular biflagellates, revealed that passive tracer particles exhibit anomalous non-Gaussian diffusion when immersed in a dilute suspension of self-motile Chlamydomonas reinhardtii algae. Qualitatively, this observation can be explained by the fact that the algae induce a fluid flow that may occasionally accelerate the colloidal tracers to relatively large velocities. A satisfactory quantitative theory of enhanced mixing in dilute active suspensions, however, is lacking at present. In particular, it is unclear how non-Gaussian signatures in the tracers' position distribution are linked to the self-propulsion mechanism of a microorganism. Here, we develop a systematic theoretical description of anomalous tracer diffusion in active suspensions, based on a simplified tracer-swimmer interaction model that captures the typical distance scaling of a microswimmer's flow field. We show that the experimentally observed non-Gaussian tails are generic and arise due to a combination of truncated L\\'evy statistics for the velocity field and algebraically decaying time correlations in the fluid. Our analytical considerations are illustrated through extensive simulations, implemented on graphics processing units to achieve the large sample sizes required for analyzing the tails of the tracer distributions.

Irwin M. Zaid; Jörn Dunkel; Julia M. Yeomans

2010-09-20T23:59:59.000Z

298

From Nanowires to Biofilms: An Exploration of Novel Mechanisms of Uranium Transformation Mediated by Geobacter Bacteria  

SciTech Connect (OSTI)

One promising strategy for the in situ bioremediation of radioactive groundwater contaminants that has been identified by the SBR Program is to stimulate the activity of dissimilatory metal-reducing microorganisms to reductively precipitate uranium and other soluble toxic metals. The reduction of U(VI) and other soluble contaminants by Geobacteraceae is directly dependent on the reduction of Fe(III) oxides, their natural electron acceptor, a process that requires the expression of Geobacter’s conductive pili (pilus nanowires). Expression of conductive pili by Geobacter cells leads to biofilm development on surfaces and to the formation of suspended biogranules, which may be physiological closer to biofilms than to planktonic cells. Biofilm development is often assumed in the subsurface, particularly at the matrix-well screen interface, but evidence of biofilms in the bulk aquifer matrix is scarce. Our preliminary results suggest, however, that biofilms develop in the subsurface and contribute to uranium transformations via sorption and reductive mechanisms. In this project we elucidated the mechanism(s) for uranium immobilization mediated by Geobacter biofilms and identified molecular markers to investigate if biofilm development is happening in the contaminated subsurface. The results provided novel insights needed in order to understand the metabolic potential and physiology of microorganisms with a known role in contaminant transformation in situ, thus having a significant positive impact in the SBR Program and providing novel concept to monitor, model, and predict biological behavior during in situ treatments.

REGUERA, GEMMA [Michigan State University

2014-01-16T23:59:59.000Z

299

ORGANIC GEOCHEMICAL STUDIES. II. THE DISTRIBUTION OF ALIPHATIC HYDROCARBONS IN ALGAE, BACTERIA, AND IN A RECENT LAKE SEDIMENT: A PRELIMINARY REPORT  

E-Print Network [OSTI]

ALIPHATIC HYDROCARBONS IN ALGAE, BACTERIA, AND IN A RECENTH F A PRELIMINARY REPORT IN ALGAE, BACTERIA, AKD IN A RECENTrests on the finding that algae have less cellulose and a

Han, Jerry; McCarthy, E.D.; Van Hoeven Jr., William; Calvin, Melvin; Bradley, W. H.

2008-01-01T23:59:59.000Z

300

Biofuel from Bacteria and Sunlight: Shewanella as an Ideal Platform for Producing Hydrocarbons  

SciTech Connect (OSTI)

Broad Funding Opportunity Announcement Project: The University of Minnesota is developing clean-burning, liquid hydrocarbon fuels from bacteria. The University is finding ways to continuously harvest hydrocarbons from a type of bacteria called Shewanella by using a photosynthetic organism to constantly feed Shewanella the sugar it needs for energy and hydrocarbon production. The two organisms live and work together as a system. Using Shewanella to produce hydrocarbon fuels offers several advantages over traditional biofuel production methods. First, it eliminates many of the time-consuming and costly steps involved in growing plants and harvesting biomass. Second, hydrocarbon biofuels resemble current petroleum-based fuels and would therefore require few changes to the existing fuel refining and distribution infrastructure in the U.S.

None

2010-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

Modeling the role of bacteria in leaching of low-grade ores  

SciTech Connect (OSTI)

A robustious structural model is developed to describe the role of bacteria in the leaching process of low-grade ores under conditions controlled by intraparticle diffusion. The main impetus behind developing this model is to provide an insight into such systems, together with a suitable framework for interpreting experimental data. The model is derived in detail with respect to reaction chemistry and the role of bacteria in catalyzing these reactions, specifically the synergism of chemistry, physics and biology in determining the overall behavior of the system. The model is used to simulate the atmospheric oxidation of iron disulfide contained in porous solids in the presence of Thiobacillus ferrooxidans (T. ferrooxidans). The experimental data are predicted well by the model, which demonstrates its applicability and supports the view that the rate of intraparticle diffusion is the controlling mechanism for this system.

Batarseh, K.I.; Stiller, A.H. (West Virginia Univ., Morgantown, WV (United States). Dept. of Chemical Engineering)

1994-10-01T23:59:59.000Z

302

Data Gathering in Networks of Bacteria Colonies: Collective Sensing and Relaying Using Molecular Communication  

E-Print Network [OSTI]

The prospect of new biological and industrial applications that require communication in micro-scale, encourages research on the design of bio-compatible communication networks using networking primitives already available in nature. One of the most promising candidates for constructing such networks is to adapt and engineer specific types of bacteria that are capable of sensing, actuation, and above all, communication with each other. In this paper, we describe a new architecture for networks of bacteria to form a data collecting network, as in traditional sensor networks. The key to this architecture is the fact that the node in the network itself is a bacterial colony; as an individual bacterium (biological agent) is a tiny unreliable element with limited capabilities. We describe such a network under two different scenarios. We study the data gathering (sensing and multihop communication) scenario as in sensor networks followed by the consensus problem in a multi-node network. We will explain as to how th...

Einolghozati, Arash; Beirami, Ahmad; Fekri, Faramarz

2012-01-01T23:59:59.000Z

303

Improved oil recovery using bacteria isolated from North Sea petroleum reservoirs  

SciTech Connect (OSTI)

During secondary oil recovery, water is injected into the formation to sweep out the residual oil. The injected water, however, follows the path of least resistance through the high-permeability zones, leaving oil in the low-permeability zones. Selective plugging of these their zones would divert the waterflood to the residual oil and thus increase the life of the well. Bacteria have been suggested as an alternative plugging agent to the current method of polymer injection. Starved bacteria can penetrate deeply into rock formations where they attach to the rock surfaces, and given the right nutrients can grow and produce exo-polymer, reducing the permeability of these zones. The application of microbial enhanced oil recovery has only been applied to shallow, cool, onshore fields to date. This study has focused on the ability of bacteria to enhance oil recovery offshore in the North Sea, where the environment can be considered extreme. A screen of produced water from oil reservoirs (and other extreme subterranean environments) was undertaken, and two bacteria were chosen for further work. These two isolates were able to grow and survive in the presence of saline formation waters at a range of temperatures above 50{degrees}C as facultative anaerobes. When a solution of isolates was passed through sandpacks and nutrients were added, significant reductions in permeabilities were achieved. This was confirmed in Clashach sandstone at 255 bar, when a reduction of 88% in permeability was obtained. Both isolates can survive nutrient starvation, which may improve penetration through the reservoir. Thus, the isolates show potential for field trials in the North Sea as plugging agents.

Davey, R.A.; Lappin-Scott, H. [Univ. of Exeter (United Kingdom)

1995-12-31T23:59:59.000Z

304

Diffusive transport without detailed balance in motile bacteria: Does microbiology need statistical physics?  

E-Print Network [OSTI]

Microbiology is the science of microbes, particularly bacteria. Many bacteria are motile: they are capable of self-propulsion. Among these, a significant class execute so-called run-and-tumble motion: they follow a fairly straight path for a certain distance, then abruptly change direction before repeating the process. This dynamics has something in common with Brownian motion (it is diffusive at large scales), and also something in contrast. Specifically, motility parameters such as the run speed and tumble rate depend on the local environment and hence can vary in space. When they do so, even if a steady state is reached, this is not generally invariant under time-reversal: the principle of detailed balance, which restores the microscopic time-reversal symmetry of systems in thermal equilibrium, is mesoscopically absent in motile bacteria. This lack of detailed balance (allowed by the flux of chemical energy that drives motility) creates pitfalls for the unwary modeller. Here I review some statistical mecha...

Cates, M E

2012-01-01T23:59:59.000Z

305

Diffusive transport without detailed balance in motile bacteria: Does microbiology need statistical physics?  

E-Print Network [OSTI]

Microbiology is the science of microbes, particularly bacteria. Many bacteria are motile: they are capable of self-propulsion. Among these, a significant class execute so-called run-and-tumble motion: they follow a fairly straight path for a certain distance, then abruptly change direction before repeating the process. This dynamics has something in common with Brownian motion (it is diffusive at large scales), and also something in contrast. Specifically, motility parameters such as the run speed and tumble rate depend on the local environment and hence can vary in space. When they do so, even if a steady state is reached, this is not generally invariant under time-reversal: the principle of detailed balance, which restores the microscopic time-reversal symmetry of systems in thermal equilibrium, is mesoscopically absent in motile bacteria. This lack of detailed balance (allowed by the flux of chemical energy that drives motility) creates pitfalls for the unwary modeller. Here I review some statistical mechanical models for bacterial motility, presenting them as a paradigm for exploring diffusion without detailed balance. I also discuss the extent to which statistical physics is useful in understanding real or potential microbiological experiments.

M. E. Cates

2012-08-20T23:59:59.000Z

306

Reprogramming Bacteria to Seek and Destroy Small Molecules (JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)  

ScienceCinema (OSTI)

Justin Gallivan, of Emory University presents a talk titled "Reprogramming Bacteria to Seek and Destroy Small Molecules" at the JGI User 7th Annual Genomics of Energy & Environment Meeting on March 21, 2012 in Walnut Creek, Calif

Gallivan, Justin [Emory University

2013-01-22T23:59:59.000Z

307

Reprogramming Bacteria to Seek and Destroy Small Molecules (JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)  

SciTech Connect (OSTI)

Justin Gallivan, of Emory University presents a talk titled "Reprogramming Bacteria to Seek and Destroy Small Molecules" at the JGI User 7th Annual Genomics of Energy & Environment Meeting on March 21, 2012 in Walnut Creek, Calif

Gallivan, Justin [Emory University] [Emory University

2012-03-21T23:59:59.000Z

308

Liquid Fuel From Bacteria: Engineering Ralstonia eutropha for Production of Isobutanol (IBT) Motor Fuel from CO2, Hydrogen, and Oxygen  

SciTech Connect (OSTI)

Electrofuels Project: MIT is using solar-derived hydrogen and common soil bacteria called Ralstonia eutropha to turn carbon dioxide (CO2) directly into biofuel. This bacteria already has the natural ability to use hydrogen and CO2 for growth. MIT is engineering the bacteria to use hydrogen to convert CO2 directly into liquid transportation fuels. Hydrogen is a flammable gas, so the MIT team is building an innovative reactor system that will safely house the bacteria and gas mixture during the fuel-creation process. The system will pump in precise mixtures of hydrogen, oxygen, and CO2, and the online fuel-recovery system will continuously capture and remove the biofuel product.

None

2010-07-15T23:59:59.000Z

309

Experimental Test Of Whether Electrostatically Charged Micro-organisms And Their Spores Contribute To The Onset Of Arcs Across Vacuum Gaps  

SciTech Connect (OSTI)

Recently it was proposed [L.R. Grisham, A. vonHalle, A.F. Carpe, Guy Rossi, K.R. Gilton, E.D. McBride, E.P. Gilson, A. Stepanov, T.N. Stevenson, Physics of Plasma 19 023107 (2012)] that one of the initiators of vacuum voltage breakdown between condu cting electrodes might be micro-organisms and their spores, previously deposited during exposure to air, which tnen become electrostatically charged when an electric potential is applied across the vacuum gap. The note describes a simple experiment to compare the number of voltage-conditioning pulses required to reach the nominal maxium operating voltage across a gap between two metallic conductors in a vacuum, comparing cases in which biological cleaning was done just prior to pump-down with cases where this was not done, with each preceded by exposure to ambient air for three days. Based upon these results, it does not appear that air-deposited microbes and their spores constitute a major pathway for arc initiation, at least for exposure periods of a few days, and for vacuum gaps of a few millimeters, in the regime where voltage holding is usually observed to vary linearly with gap distance

none,; Grisham, Larry R.

2014-02-24T23:59:59.000Z

310

Hormetic effect of ionic liquid 1-ethyl-3-methylimidazolium acetate on bacteria  

DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

The biological effect of ionic liquids (ILs) is one of the highly debated topics as they are being contemplated for various industrial applications. 1-ethyl-2-methylimidazolium acetate ([EMIM][Ac]) showed remarkable hormesis on anaerobic Clostridium sp. and aerobic Psueudomonas putida. Bacterial growth was stimulated at up to 2.5 g L-1 and inhibited at > 2.5 g L-1 of ([EMIM][Ac]). The growth of Clostridium sp. and P. putida were higher by 0.4 and 4-fold respectively, in the presense of 0.5 g L-1 of ([EMIM][Ac]). Assessment of the effect of [EMIM][Ac] under different growth conditions showed that the hormesis of [EMIM][Ac] was mediated via regulation of medium pH. Hormetic effect of [EMIM][Ac] was evident only in medium with poor buffering capacity and in the presence of a fermentable substrate as the carbon source. The hormetic effect of [EMIM][Ac] on bacterial growth is most likely associated with the buffering capacity of acetate anion. These observations have implications in ILs toxicity studies and ecological risk assessment.

Nancharaiah, Y. V. [Bhabha Atomic Research Center, Kalpakkam (India). Biofouling and Biofilm Processes Sect.; Francis, A. J. [Brookhaven National Laboratory (BNL), Upton, NY (United States). Environmental Sciences Dept.; POSTECH, Pohang (Korea, Rep. of). Div. of Advanced Nuclear Engineering

2015-06-01T23:59:59.000Z

311

Effects of drilling fluids on marine bacteria from a Nigerian offshore oilfield  

SciTech Connect (OSTI)

Two marine bacterial isolates from drill mud cuttings obtained from Agbara oilfield, Staphylococcus sp. and Bacillus sp., were cultured aerobically in the presence of varying concentrations (0, 25, 50, and 75 {mu}g/ml) of drilling fluids to determine the effects of concentration of toxicants on their growth. With the exception of Clairsol, Enviromul, and Bariod mineral oil, which had little or no effect, the exponential growth of Bacillus sp. was depressed by all other test chemicals. Additionally, all test chemicals except Clairsol had no effect on lag phase of growth of Bacillus sp. With Staphylococcus sp. the depressive effect on the exponential phase of growth was shown by almost all test chemicals. There was enhancement of both growth rate and generation times of Staphylococcus sp. and decrease of those of Bacillus sp. with increasing concentrations of drilling fluids. These results show that while some drilling fluids may be stimulatory or depressive to bacterial growth, others may be without effect. 23 refs., 6 figs., 3 tabs.

Okpokwasil, G.C.; Nnubia, C. [Univ. of Prot Harcourt (Nigeria)

1995-11-01T23:59:59.000Z

312

MOLECULAR APPROACHES FOR IN SITU IDENTIFCIATION OF NITRATE UTILIZATION BY MARINE BACTERIA AND PHYTOPLANKTON  

SciTech Connect (OSTI)

Traditionally, the importance of inorganic nitrogen (N) for the nutrition and growth of marine phytoplankton has been recognized, while inorganic N utilization by bacteria has received less attention. Likewise, organic N has been thought to be important for heterotrophic organisms but not for phytoplankton. However, accumulating evidence suggests that bacteria compete with phytoplankton for nitrate (NO3-) and other N species. The consequences of this competition may have a profound effect on the flux of N, and therefore carbon (C), in ocean margins. Because it has been difficult to differentiate between N uptake by heterotrophic bacterioplankton versus autotrophic phytoplankton, the processes that control N utilization, and the consequences of these competitive interactions, have traditionally been difficult to study. Significant bacterial utilization of DIN may have a profound effect on the flux of N and C in the water column because sinks for dissolved N that do not incorporate inorganic C represent mechanisms that reduce the atmospheric CO2 drawdown via the ?biological pump? and limit the flux of POC from the euphotic zone. This project was active over the period of 1998-2007 with support from the DOE Biotechnology Investigations ? Ocean Margins Program (BI-OMP). Over this period we developed a tool kit of molecular methods (PCR, RT-PCR, Q-PCR, QRT-PCR, and TRFLP) and combined isotope mass spectrometry and flow-cytometric approaches that allow selective isolation, characterization, and study of the diversity and genetic expression (mRNA) of the structural gene responsible for the assimilation of NO3- by heterotrophic bacteria (nasA). As a result of these studies we discovered that bacteria capable of assimilating NO3- are ubiquitous in marine waters, that the nasA gene is expressed in these environments, that heterotrophic bacteria can account for a significant fraction of total DIN uptake in different ocean margin systems, that the expression of nasA is differentially regulated in genetically distinct NO3- assimilating bacteria, and that the best predictors of nasA gene expression are either NO3- concentration or NO3- uptake rates. These studies provide convincing evidence of the importance of bacterial utilization of NO3-, insight into controlling processes, and provide a rich dataset that are being used to develop linked C and N modeling components necessary to evaluate the significance of bacterial DIN utilization to global C cycling. Furthermore, as a result of BI-OMP funding we made exciting strides towards institutionalizing a research and education based collaboration between the Skidaway Institute of Oceanography (SkIO) and Savannah State University (SSU), an historically black university within the University System of Georgia with undergraduate and now graduate programs in marine science. The BI-OMP program, in addition to supporting undergraduate (24) graduate (10) and postdoctoral (2) students, contributed to the development of a new graduate program in Marine Sciences at SSU that remains an important legacy of this project. The long-term goals of these collaborations are to increase the capacity for marine biotechnology research and to increase representation of minorities in marine, environmental and biotechnological sciences.

Frischer, Marc E. [Skidaway Institute of Oceanography; Verity, Peter G.; Gilligan, Mathew R.; Bronk, Deborah A.; Zehr, Jonathan P.; Booth, Melissa G.

2013-09-12T23:59:59.000Z

313

Exploration of Simple Analytical Approaches for Rapid Detection of Pathogenic Bacteria  

SciTech Connect (OSTI)

Many of the current methods for pathogenic bacterial detection require long sample-preparation and analysis time, as well as complex instrumentation. This dissertation explores simple analytical approaches (e.g., flow cytometry and diffuse reflectance spectroscopy) that may be applied towards ideal requirements of a microbial detection system, through method and instrumentation development, and by the creation and characterization of immunosensing platforms. This dissertation is organized into six sections. In the general Introduction section a literature review on several of the key aspects of this work is presented. First, different approaches for detection of pathogenic bacteria will be reviewed, with a comparison of the relative strengths and weaknesses of each approach, A general overview regarding diffuse reflectance spectroscopy is then presented. Next, the structure and function of self-assembled monolayers (SAMs) formed from organosulfur molecules at gold and micrometer and sub-micrometer patterning of biomolecules using SAMs will be discussed. This section is followed by four research chapters, presented as separate manuscripts. Chapter 1 describes the efforts and challenges towards the creation of imunosensing platforms that exploit the flexibility and structural stability of SAMs of thiols at gold. 1H, 1H, 2H, 2H-perfluorodecyl-1-thiol SAM (PFDT) and dithio-bis(succinimidyl propionate)-(DSP)-derived SAMs were used to construct the platform. Chapter 2 describes the characterization of the PFDT- and DSP-derived SAMs, and the architectures formed when it is coupled to antibodies as well as target bacteria. These studies used infrared reflection spectroscopy (IRS), X-ray photoelectron spectroscopy (XPS), and electrochemical quartz crystal microbalance (EQCM), Chapter 3 presents a new sensitive, and portable diffuse reflection based technique for the rapid identification and quantification of pathogenic bacteria. Chapter 4 reports research efforts in the construction and evaluation of a prototype flow cytometry based cell detector and enumerator. This final research chapter is followed by a general summation and future prospectus section that concludes this dissertation.

Salma Rahman

2005-12-17T23:59:59.000Z

314

Soft inertial microfluidics for high throughput separation of bacteria from human blood cells  

SciTech Connect (OSTI)

We developed a new approach to separate bacteria from human blood cells based on soft inertial force induced migration with flow defined curved and focused sample flow inside a microfluidic device. This approach relies on a combination of an asymmetrical sheath flow and proper channel geometry to generate a soft inertial force on the sample fluid in the curved and focused sample flow segment to deflect larger particles away while the smaller ones are kept on or near the original flow streamline. The curved and focused sample flow and inertial effect were visualized and verified using a fluorescent dye primed in the device. First the particle behavior was studied in detail using 9.9 and 1.0 {micro}m particles with a polymer-based prototype. The prototype device is compact with an active size of 3 mm{sup 2}. The soft inertial effect and deflection distance were proportional to the fluid Reynolds number (Re) and particle Reynolds number (Re{sub p}), respectively. We successfully demonstrated separation of bacteria (Escherichia coli) from human red blood cells at high cell concentrations (above 10{sup 8}/mL), using a sample flow rate of up to 18 {micro}L/min. This resulted in at least a 300-fold enrichment of bacteria at a wide range of flow rates with a controlled flow spreading. The separated cells were proven to be viable. Proteins from fractions before and after cell separation were analyzed by gel electrophoresis and staining to verify the removal of red blood cell proteins from the bacterial cell fraction. This novel microfluidic process is robust, reproducible, simple to perform, and has a high throughput compared to other cell sorting systems. Microfluidic systems based on these principles could easily be manufactured for clinical laboratory and biomedical applications.

Wu, Zhigang; Willing, Ben; Bjerketorp, Joakim; Jansson, Janet K.; Hjort, Klas

2009-01-05T23:59:59.000Z

315

A technique for quantitative and qualitative viewing of aquatic bacteria using scanning electron microscopy  

E-Print Network [OSTI]

microscopic enumeration techniques. Water samples are concentrated on pre-wetted (Triton X-100) Nuclepore filters (0. 2 um pore size) to prov1de a uniform distri- bution of bacteria on the filter surface and vacuum filtered (660 Torr). The filter... is transferred to a petri dish containing filter paper soaked 1n 2% glutaraldehyde and the bacter1a are fixed for one hour. Dehydration 1s performed by transferr1ng the filters through a series of petri dishes conta1ning filter paper saturated with 25, 50, 75...

Dreier, Thomas Michael

2012-06-07T23:59:59.000Z

316

Light-harvesting in bacteria exploits a critical interplay between transport and trapping dynamics  

E-Print Network [OSTI]

Light-harvesting bacteria Rhodospirillum Photometricum were recently found to adopt strikingly different architectures depending on illumination conditions. We present analytic and numerical calculations which explain this observation by quantifying a dynamical interplay between excitation transfer kinetics and reaction center cycling. High light-intensity membranes (HLIM) exploit dissipation as a photo-protective mechanism, thereby safeguarding a steady supply of chemical energy, while low light-intensity membranes (LLIM) efficiently process unused illumination intensity by channelling it to open reaction centers. More generally, our analysis elucidates and quantifies the trade-offs in natural network design for solar energy conversion.

Felipe Caycedo-Soler; Ferney J. Rodriguez; Luis Quiroga; Neil F. Johnson

2010-03-11T23:59:59.000Z

317

Chemotactic behavior of deep subsurface bacteria toward carbohydrates, amino acids and a chlorinated alkene  

SciTech Connect (OSTI)

The chemotactic behavior of deep terrestrial subsurface bacteria toward amino acids, carbohydrates and trichloroethylene was assayed using a modification of the capillary method and bacterial enumeration by acridine orange direct counts. Eleven isolates of bacteria isolated from six different geological formations were investigated. A bimodal response rather than an absolute positive or negative response was observed in most assays. Most of the isolates were positively chemotactic to low concentrations of substrates and were repelled by high concentrations of the same substrate. However, this was not the case for trichloroethylene (TCE) which was mostly an attractant and elicited the highest responses in all the isolates when compared with amino acids and carbohydrates. The movement rates of these isolates in aseptic subsurface sediments in the absence and presence of TCE were also determined using a laboratory model. All of the isolates showed distinct response range, peak, and threshold concentrations when exposed to the same substrates suggesting that they are possibly different species as has been inferred from DNA homology studies. 101 refs., 4 figs., 57 tabs.

Lopez de Victoria, G. (Puerto Rico Univ., Rio Piedras (Puerto Rico). Dept. of Biology)

1989-02-01T23:59:59.000Z

318

Structural similarities between biogenic uraninites produced by phylogenetically and metabolically diverse bacteria.  

SciTech Connect (OSTI)

While the product of microbial uranium reduction is often reported to be“UO2”, a comprehensive characterization including stoichiometry and unit cell determination is available for only one Shewanella species. Here, we compare the products of batch uranyl reduction by a collection of dissimilatory metal- and sulfate-reducing bacteria of the genera Shewanella, Geobacter, Anaeromyxobacter, and Desulfovibrio under similar laboratory conditions. Our results demonstrate that U(VI) bioreduction by this assortment of commonly studied, environmentally relevant bacteria leads to the precipitation of uraninite with a composition between UO2.00 and UO2.075, regardless of phylogenetic or metabolic diversity. Coupled analyses, including electron microscopy, X-ray absorption spectroscopy, and powder diffraction, confirm that structurally and chemically analogous uraninite solids are produced. These biogenic uraninites have particle diameters of about 2-3 nm and lattice constants consistent with UO2.0 and exhibit a high degree of intermediate-range order. Results indicate that phylogenetic and metabolic variability within delta- and gamma-proteobacteria has little effect on nascent biouraninite structure or crystal size under the investigated conditions.

Sharp, Jonathan; Schofield, Eleanor J.; Veeramani, Harish; Suvorova, Elena; Kennedy, David W.; Marshall, Matthew J.; Mehta, Apurva; Bargar, John R.; Bernier-Latmani, Rizlan

2009-11-01T23:59:59.000Z

319

The Mannitol Operon Repressor MTIR belongs to a new class of transcription regulators in bacteria.  

SciTech Connect (OSTI)

Many bacteria express phosphoenolpyruvate-dependent phosphotransferase systems (PTS). The mannitol-specific PTS catalyze the uptake and phosphorylation of d-mannitol. The uptake system comprises several genes encoded in the single operon. The expression of the mannitol operon is regulated by a proposed transcriptional factor, mannitol operon repressor (MtlR) that was first studied in Escherichia coli. Here we report the first crystal structures of MtlR from Vibrio parahemeolyticus (Vp-MtlR) and its homolog YggD protein from Shigella flexneri (Sf-YggD). MtlR and YggD belong to the same protein family (Pfam05068). Although Vp-MtlR and Sf-YggD share low sequence identity (22%), their overall structures are very similar, representing a novel all {alpha}-helical fold, and indicate similar function. However, their lack of any known DNA-binding structural motifs and their unfavorable electrostatic properties imply that MtlR/YggD are unlikely to bind a specific DNA operator directly as proposed earlier. This structural observation is further corroborated by in vitro DNA-binding studies of E. coli MtlR (Ec-MtlR), which detected no interaction of Ec-MtlR with the well characterized mannitol operator/promoter region. Therefore, MtlR/YggD belongs to a new class of transcription factors in bacteria that may regulate gene expression indirectly as a part of a larger transcriptional complex.

Tan, K.; Borovilos, M.; Zhou, M; Horer, S; Clancy, S; Moy, S; Volkart, LL; Sassoon, J; Baumann, U; Joachimiak, A (Biosciences Division); (Univ. of Berne)

2009-12-25T23:59:59.000Z

320

Iron-reducing bacteria accumulate ferric oxyhydroxide nanoparticle aggregates that may support planktonic growth  

SciTech Connect (OSTI)

Iron-reducing bacteria (FeRB) play key roles in anaerobic metal and carbon cycling and carry out biogeochemical transformations that can be harnessed for environmental bioremediation. A subset of FeRB require direct contact with Fe(III) bearing minerals for dissimilatory growth, yet these bacteria must move between mineral particles. Further, they proliferate in planktonic consortia during biostimulation experiments. Thus, a key question is how such organisms can sustain growth under these conditions. Here we characterized planktonic microbial communities sampled from an aquifer in Rifle, Colorado, USA close to the peak of iron reduction following in situ acetate amendment. Samples were cryo-plunged on site and subsequently examined using correlated 2- and 3- dimensional cryogenic transmission electron microscopy (cryo-TEM) and scanning transmission X-ray microscopy (STXM). Most cells had their outer membranes decorated with up to 150 nm diameter aggregates composed of a few nm wide amorphous, Fe-rich nanoparticles. Fluorescent in situ hybridization of lineage-specific probes applied to rRNA of cells subsequently imaged via cryo-TEM identified Geobacter spp., a well studied group of FeRB. STXM results at the Fe L2,3 absorption edges indicate that nanoparticle aggregates contain a variable mixture of Fe(II)-Fe(III), and are generally enriched in Fe(III). Geobacter bemidjiensis cultivated anaerobically in the laboratory on acetate and hydrous ferric oxyhydroxides also accumulated mixed valence nanoparticle aggregates. In field-collected samples, FeRB with a wide variety of morphologies were associated with nano-aggregates, indicating that cell-surface Fe(III) accumulation may be a general mechanism by which FeRB can grow while in planktonic suspension.

Luef, Birgit; Fakra, Sirine C.; Csencsits, Roseann; Wrighton, Kelly C.; Williams, Kenneth H.; Wilkins, Michael J.; Downing, Kenneth H.; Long, Philip E.; Comolli, Luis R.; Banfield, Jillian F.

2013-02-04T23:59:59.000Z

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

How do microbial fuel cells (MFCs) work? Bacteria need energy to survive, in the same way that humans need food to  

E-Print Network [OSTI]

much does it cost to treat wastewater? In the U.S. alone, the cost of treating 33 billion gallonsHow do microbial fuel cells (MFCs) work? Bacteria need energy to survive, in the same way that humans need food to live. Bacteria get this energy in a two-step process. The first step requires

Lee, Dongwon

322

Protecting Our Water: Tracking Sources of Bacterial Contamination Numerous surface waterbodies in Texas are classified as having high levels of fecal coliform bacteria, an indicator of fecal pollution.  

E-Print Network [OSTI]

in Texas are classified as having high levels of fecal coliform bacteria, an indicator of fecal pollution. coli and other fecal coliform bacteria do not provide information on whether the source of pollution of pollution need to be identified to implement effective pollution control strategies to improve water quality

323

Excitonic energy transfer in light-harvesting complexes in purple bacteria Jun Ye, Kewei Sun, Yang Zhao, Yunjin Yu, Chee Kong Lee et al.  

E-Print Network [OSTI]

Excitonic energy transfer in light-harvesting complexes in purple bacteria Jun Ye, Kewei Sun, Yang-harvesting complexes in purple bacteria Jun Ye,1 Kewei Sun,1 Yang Zhao,1,a) Yunjin Yu,1,2 Chee Kong Lee,1 and Jianshu

Cao, Jianshu

324

Bugs boost Cold War clean-up: Bacteria could scrub uranium from sites contaminated decades ago. updated at midnight GMTtoday is friday, november 14  

E-Print Network [OSTI]

2003 · Fungus catches radioactive fallout 8 May 2002 · Depleted uranium soils battlefields 12 MarchBugs boost Cold War clean-up: Bacteria could scrub uranium from sites contaminated decades ago boost Cold War clean-up Bacteria could scrub uranium from sites contaminated decades ago. 13 October

Lovley, Derek

325

Digestion of milk protein and methanol-grown bacteria protein in the preruminant calf. I. Kinetics and balance in the terminal  

E-Print Network [OSTI]

Digestion of milk protein and methanol-grown bacteria protein in the preruminant calf. I. Kinetics the digestion of two milk replacers (control and bacteria), containing different protein sources, in the end of the small intestine and of the digestive tract in the preruminant calf. The protein in the control diet

Paris-Sud XI, Université de

326

Corrosion Protection due to Bacteria /Metal Interactions E. Kus1, K. H. Nealson2 and F. Mansfeld1  

E-Print Network [OSTI]

Corrosion Protection due to Bacteria /Metal Interactions E. Kus1, K. H. Nealson2 and F. Mansfeld1 1. Corrosion and Environmental Effects Laboratory (CEEL) The Mork Family Department of Chemical Engineering Microbiologically influenced corrosion (MIC) refers to the increase of the corrosion rate of metals due to microbial

Southern California, University of

327

University of Minnesota study of exposure of animal manure applicators to bacteria (S. aureus, MRSA) of swine origin  

E-Print Network [OSTI]

University of Minnesota study of exposure of animal manure applicators to bacteria (S. aureus, MRSA to certain antibiotics, and those resistant to the drug methicillin (known as MRSA ­ methicillin resistant S be a reservoir of MRSA. Furthermore, people with direct swine contact have an increased high likelihood

Netoff, Theoden

328

Digestion of milk protein and methanol-grown bacteria protein in the preruminant calf. II. Amino acid composition of  

E-Print Network [OSTI]

Digestion of milk protein and methanol-grown bacteria protein in the preruminant calf. II. Amino Beaumont, France. Summary. The aim of this trial was to study the balance of the amino acid digestion digestibility of all the amino acids assayed was lower in the terminal small intestine than in the whole

Paris-Sud XI, Université de

329

When fish die, bacteria or the enzymes they produce invade the flesh of fish. This process produces toxic  

E-Print Network [OSTI]

ABSTRACT When fish die, bacteria or the enzymes they produce invade the flesh of fish. This process produces toxic compounds in the fish and the fish becomes spoiled. Fourier Transform Infrared spectroscopy neural network (ANN) for the development of an ANN based FT-IR Screening System for fish

Michel, Howard E.

330

Detecting bacteria and Determining Their Susceptibility to Antibiotics by Stochastic Confinement in Nanoliter Droplets using Plug-Based Microfluidics  

SciTech Connect (OSTI)

This article describes plug-based microfluidic technology that enables rapid detection and drug susceptibility screening of bacteria in samples, including complex biological matrices, without pre-incubation. Unlike conventional bacterial culture and detection methods, which rely on incubation of a sample to increase the concentration of bacteria to detectable levels, this method confines individual bacteria into droplets nanoliters in volume. When single cells are confined into plugs of small volume such that the loading is less than one bacterium per plug, the detection time is proportional to plug volume. Confinement increases cell density and allows released molecules to accumulate around the cell, eliminating the pre-incubation step and reducing the time required to detect the bacteria. We refer to this approach as stochastic confinement. Using the microfluidic hybrid method, this technology was used to determine the antibiogram - or chart of antibiotic sensitivity - of methicillin-resistant Staphylococcus aureus (MRSA) to many antibiotics in a single experiment and to measure the minimal inhibitory concentration (MIC) of the drug cefoxitin (CFX) against this strain. In addition, this technology was used to distinguish between sensitive and resistant strains of S. aureus in samples of human blood plasma. High-throughput microfluidic techniques combined with single-cell measurements also enable multiple tests to be performed simultaneously on a single sample containing bacteria. This technology may provide a method of rapid and effective patient-specific treatment of bacterial infections and could be extended to a variety of applications that require multiple functional tests of bacterial samples on reduced timescales.

Boedicker, J.; Li, L; Kline, T; Ismagilov, R

2008-01-01T23:59:59.000Z

331

Dissimilatory Metabolism of Nitrogen Oxides in Bacteria:Comparative Reconstruction of Transcriptional Networks  

SciTech Connect (OSTI)

Bacterial response to nitric oxide (NO) is of major importance since NO is an obligatory intermediate of the nitrogen cycle. Transcriptional regulation of the dissimilatory nitric oxides metabolism in bacteria is diverse and involves FNR-like transcription factors HcpR, DNR and NnrR, two-component systems NarXL and NarQP, NO-responsive activator NorR, and nitrite sensitive repressor NsrR. Using comparative genomics approaches we predict DNA-binding signals for these transcriptional factors and describe corresponding regulons in available bacterial genomes. Within the FNR family of regulators, we observed a correlation of two specificity-determining amino acids and contacting bases in corresponding DNA signal. Highly conserved regulon HcpR for the hybrid cluster protein and some other redox enzymes is present in diverse anaerobic bacteria including Clostridia, Thermotogales and delta-proteobacteria. NnrR and DNR control denitrification in alpha- and beta-proteobacteria, respectively. Sigma-54-dependent NorR regulon found in some gamma- and beta-proteobacteria contains various enzymes involved in the NO detoxification. Repressor NsrR, which was previously known to control only nitrite reductase operon in Nitrosomonas spp., appears to be the master regulator of the nitric oxides metabolism not only in most gamma- and beta-proteobacteria (including well-studied species like Escherichia coli), but also in Gram-positive Bacillus and Streptomyces species. Positional analysis and comparison of regulatory regions of NO detoxification genes allows us to propose the candidate NsrR-binding signal. The most conserved member of the predicted NsrR regulon is the NO-detoxifying flavohemoglobin Hmp. In enterobacteria, the regulon includes also two nitrite-responsive loci, nipAB (hcp-hcr) and nipC(dnrN), thus confirming the identity of the effector, i.e., nitrite. The proposed NsrR regulons in Neisseria and some other species are extended to include denitrification genes. As the result, we demonstrate considerable interconnection between various nitrogen-oxides-responsive regulatory systems for the denitrification and NO detoxification genes and evolutionary plasticity of this transcriptional network.

Rodionov, Dmitry A.; Dubchak, Inna L.; Arkin, Adam P.; Alm, EricJ.; Gelfand, Mikhail S.

2005-09-01T23:59:59.000Z

332

In-Situ Survival Mechanisms of U and Tc Reducing Bacteria in Contaminated Sediments  

SciTech Connect (OSTI)

Desulfovibrio desulfuricans G20 and Shewanella oneidensis MR-1 are model subsurface organisms for studying genes involving in situ radionuclide transformation and sediment survival. Our research objective for this project has been to develop a signature-tagged mutagenesis (STM) procedure and use it to identify mutants in genes of these subsurface bacteria involved in sediment survival and radionuclide reduction. The mutant genes identified in these studies allow us for the first time to describe at the genetic level microbial processes that are actually being used by environmental bacteria while growing in their natural ecosystems. Identification of these genes revealed facets of microbial physiology and ecology that are not accessible through laboratory studies. Ultimately, this information may be used to optimize bioremediation or other engineered microbial processes. Furthermore, the identification of a mutant in a gene conferring multidrug resistance in strain MR-1 shows that this widespread mechanism of antibiotic resistance, likely has its origins as a mechanism of bacterial defense against naturally occurring toxins. Studies with D. desulfuricans G20: The STM procedure first involved generating a library of 5760 G20 mutants and screening for potential non-survivors in subsurface sediment microcosms. After two rounds of screening, a total of 117 mutants were confirmed to be true non-survivors. 97 transposon insertion regions have been sequenced to date. Upon further analysis of these mutants, we classified the sediment survival genes into COG functional categories. STM mutant insertions were located in genes encoding proteins related to metabolism (33%), cellular processes (42%), and information storage and processing (17%). We also noted 8% of STM mutants identified had insertions in genes for hypothetical proteins or unknown functions. Interestingly, at least 64 of these genes encode cytoplasmic proteins, 46 encode inner membrane proteins, and only 7 encode periplasmic space and outer membrane associated proteins. Through blast search analysis, we also showed that 81 out of 94 proteins shown to be important in sediment survival have homologs in D. vulgaris, 70 have homologs in Geobacter metallireducens, and 69 have homologs in Geobacter sulfurreducens PCA. Some interesting proteins include ribonucleotide reductase and chemotaxis related proteins. Ribonucleotide reductase catalyzes the reductive synthesis of deoxyribonucleotides from their corresponding ribonucleotides, providing the precursors necessary for DNA synthesis. Two ribonucleotide reductase genes (nrdE, nrdD) were found to be essential for G20 survival in the sediment, but not essential for growth in the lactate-sulfate medium. Bacterial methyl-accepting chemotaxis proteins (MCP) respond to changes in the concentration of attractants and repellents in the environment.

Krumholz, Lee R.

2005-06-01T23:59:59.000Z

333

Diversity and characterization of sulfate-reducing bacteria in groundwater at a uranium mill tailings site  

E-Print Network [OSTI]

plays a role in both natural attenuation and accelerated bioremediation of uranium-contaminated sites. To realize bioremediation potential and accurately predict natural attenuation, it is important to first understand the microbial diversity of such sites. In this paper, the distribution of sulfate-reducing bacteria (SRB) in contaminated groundwater associated with a uranium mill tailings disposal site at Shiprock, N.Mex., was investigated. Two culture-independent analyses were employed: sequencing of clone libraries of PCR-amplified dissimilatory sulfite reductase (DSR) gene fragments and phospholipid fatty acid (PLFA) biomarker analysis. A remarkable diversity among the DSR sequences was revealed, including sequences from ?-Proteobacteria, gram-positive organisms, and the Nitrospira division. PLFA analysis detected at least 52 different mid-chain-branched saturate PLFA and included a high proportion of 10me16:0. Desulfotomaculum and Desulfotomaculum-like sequences were the most dominant DSR genes detected. Those belonging to SRB within ?-Proteobacteria were mainly recovered from low-uranium (1,500 ppb) sites. Logistic regression showed a significant influence of uranium concentration over the dominance of this cluster of sequences (P ? 0.0001). This strong association indicates that Desulfotomaculum has remarkable

Yun-juan Chang; Aaron D. Peacock; Philip E. Long; John R. Stephen; James P. Mckinley; Sarah J. Macnaughton; A. K. M. Anwar Hussain; Arnold M. Saxton; David C. White

2001-01-01T23:59:59.000Z

334

Stuttering Min oscillations within E. coli bacteria: A stochastic polymerization model  

E-Print Network [OSTI]

We have developed a 3D off-lattice stochastic polymerization model to study subcellular oscillation of Min proteins in the bacteria Escherichia coli, and used it to investigate the experimental phenomenon of Min oscillation stuttering. Stuttering was affected by the rate of immediate rebinding of MinE released from depolymerizing filament tips (processivity), protection of depolymerizing filament tips from MinD binding, and fragmentation of MinD filaments due to MinE. Each of processivity, protection, and fragmentation reduces stuttering, speeds oscillations, and reduces MinD filament lengths. Neither processivity or tip-protection were, on their own, sufficient to produce fast stutter-free oscillations. While filament fragmentation could, on its own, lead to fast oscillations with infrequent stuttering; high levels of fragmentation degraded oscillations. The infrequent stuttering observed in standard Min oscillations are consistent with short filaments of MinD, while we expect that mutants that exhibit higher stuttering frequencies will exhibit longer MinD filaments. Increased stuttering rate may be a useful diagnostic to find observable MinD polymerization in experimental conditions.

Supratim Sengupta; Julien Derr; Anirban Sain; Andrew D. Rutenberg

2012-07-23T23:59:59.000Z

335

Active transport, substrate specificity, and methylation of Hg(II) in anaerobic bacteria  

SciTech Connect (OSTI)

The formation of methylmercury (MeHg), which is biomagnified in aquatic food chains and poses a risk to human health, is effected by some iron- and sulfate-reducing bacteria (FeRB and SRB) in anaerobic environments. However, very little is known regarding the mechanism of uptake of inorganic Hg by these organisms, in part because of the inherent difficulty in measuring the intracellular Hg concentration. By using the FeRB Geobacter sulfurreducens and the SRB Desulfovibrio desulfuricans ND132 as model organisms, we demonstrate that Hg(II) uptake occurs by active transport. We also establish that Hg(II) uptake by G. sulfurreducens is highly dependent on the characteristics of the thiols that bind Hg(II) in the external medium, with some thiols promoting uptake and methylation and others inhibiting both. The Hg(II) uptake system of D. desulfuricans has a higher affinity than that of G. sulfurreducens and promotes Hg methylation in the presence of stronger complexing thiols. We observed a tight coupling between Hg methylation and MeHg export from the cell, suggesting that these two processes may serve to avoid the build up and toxicity of cellular Hg. Our results bring up the question of whether cellular Hg uptake is specific for Hg(II) or accidental, occurring via some essential metal importer. Our data also point at Hg(II) complexation by thiols as an important factor controlling Hg methylation in anaerobic environments.

Schasfer, Jeffra [Princeton University; Rocks, Sara [Princeton University; Zheng, Wang [ORNL; Liang, Liyuan [ORNL; Gu, Baohua [ORNL; Morel, Francois M [ORNL

2011-01-01T23:59:59.000Z

336

Composition, Reactivity, and Regulations of Extracellular Metal-Reducing Structures (Bacterial Nanowires) Produced by Dissimilatory Metal Reducing Bacteria  

SciTech Connect (OSTI)

This research proposal seeks to describe the composition and function of electrically conductive appendages known as bacterial nanowires. This project targets bacterial nanowires produced by dissimilatory metal reducing bacteria Shewanella and Geobacter. Specifically, this project will investigate the role of these structures in the reductive transformation of iron oxides as solid phase electron acceptors, as well as uranium as a dissolved electron acceptor that forms nanocrystalline particles of uraninite upon reduction.

Scholten, Johannes

2006-06-01T23:59:59.000Z

337

Deduction and Analysis of the Interacting Stress Response Pathways of Metal/Radionuclide-reducing Bacteria  

SciTech Connect (OSTI)

Project Title: Deduction and Analysis of the Interacting Stress Response Pathways of Metal/Radionuclide-reducing Bacteria DOE Grant Number: DE-FG02-06ER64205 Principal Investigator: Jizhong (Joe) Zhou (University of Oklahoma) Key members: Zhili He, Aifen Zhou, Christopher Hemme, Joy Van Nostrand, Ye Deng, and Qichao Tu Collaborators: Terry Hazen, Judy Wall, Adam Arkin, Matthew Fields, Aindrila Mukhopadhyay, and David Stahl Summary Three major objectives have been conducted in the Zhou group at the University of Oklahoma (OU): (i) understanding of gene function, regulation, network and evolution of Desulfovibrio vugaris Hildenborough in response to environmental stresses, (ii) development of metagenomics technologies for microbial community analysis, and (iii) functional characterization of microbial communities with metagenomic approaches. In the past a few years, we characterized four CRP/FNR regulators, sequenced ancestor and evolved D. vulgaris strains, and functionally analyzed those mutated genes identified in salt-adapted strains. Also, a new version of GeoChip 4.0 has been developed, which also includes stress response genes (StressChip), and a random matrix theory-based conceptual framework for identifying functional molecular ecological networks has been developed with the high throughput functional gene array hybridization data as well as pyrosequencing data from 16S rRNA genes. In addition, GeoChip and sequencing technologies as well as network analysis approaches have been used to analyze microbial communities from different habitats. Those studies provide a comprehensive understanding of gene function, regulation, network, and evolution in D. vulgaris, and microbial community diversity, composition and structure as well as their linkages with environmental factors and ecosystem functioning, which has resulted in more than 60 publications.

Zhou, Jizhong [University of Oklahoma; He, Zhili [University of Oklahoma

2010-02-28T23:59:59.000Z

338

Diversity and characterization of sulfate-reducing bacteria in groundwater at a uranium mill tailings site  

SciTech Connect (OSTI)

Microbially mediated reduction and immobilization of U(VI) to U(TV) plays a role in both natural attenuation and accelerated bioremediation of uranium contaminated sites. To realize bioremediation potential and accurately predict natural attenuation, it is important to first understand the microbial diversity of such sites. In this paper, the distribution of sulfate-reducing bacteria (SRB) in contaminated groundwater associated with a uranium mill tailings disposal site at Shiprock, N.Mex,, was investigated. Two culture-independent analyses were employed: sequencing of clone libraries of PCR-amplified dissimilatory sulfite reductase (DSR) gene fragments and phospholipid fatty acid (PLFA) biomarker analysis. A remarkable diversity among the DSR sequences was revealed, including sequences from F-Proteobacteria, gram-positive organisms, and the Nitrospira division. PLFA analysis detected at least,52 different mid-chain-branched saturate PLFA and included a high proportion of 10me16:0, Desulfotomaculum and Desulfotomaculum-like sequences were the most dominant DSR genes detected. Those belonging to SRB within F-Proteobacteria were mainly recovered from low-uranium (less than or equal to 302 ppb) samples. One Desulfotomaculum like sequence cluster overwhelmingly dominated high-U (> 1,500 ppb) sites. Logistic regression showed a significant influence of uranium concentration over the dominance of this cluster of sequences (P= 0.0001), This strong association indicates that Desulfotomaculum has remarkable tolerance and adaptation to high levels of uranium and suggests the organism's possible involvement in natural attenuation of uranium. The in situ activity level of Desulfotomaculum in uranium-contaminated environments and its comparison to the activities of other SRB and other functional groups should be an important area for future research.

Chang, Yun-Juan (Unknown); Peacock, A D. (Tennessee, Univ Of); Long, Philip E. (BATTELLE (PACIFIC NW LAB)); Stephen, John R. (Unknown); McKinley, James P. (BATTELLE (PACIFIC NW LAB)); Mcnaughton, Sarah J. (Unknown); Hussain, A K M A.; Saxton, A M.; White, D C. (Unknown)

2000-12-01T23:59:59.000Z

339

Lithotrophic iron-oxidizing bacteria produce organic stalks to control mineral growth: implications for biosignature formation  

SciTech Connect (OSTI)

Neutrophilic Fe-oxidizing bacteria (FeOB) are often identified by their distinctive morphologies, such as the extracellular twisted ribbon-like stalks formed by Gallionella ferruginea or Mariprofundus ferrooxydans. Similar filaments preserved in silica are often identified as FeOB fossils in rocks. Although it is assumed that twisted iron stalks are indicative of FeOB, the stalk's metabolic role has not been established. To this end, we studied the marine FeOB M. ferrooxydans by light, X-ray and electron microscopy. Using time-lapse light microscopy, we observed cells excreting stalks during growth (averaging 2.2 {micro}m h(-1)). Scanning transmission X-ray microscopy and near-edge X-ray absorption fine structure (NEXAFS) spectroscopy show that stalks are Fe(III)-rich, whereas cells are low in Fe. Transmission electron microscopy reveals that stalks are composed of several fibrils, which contain few-nanometer-sized iron oxyhydroxide crystals. Lepidocrocite crystals that nucleated on the fibril surface are much larger ({approx}100 nm), suggesting that mineral growth within fibrils is retarded, relative to sites surrounding fibrils. C and N 1s NEXAFS spectroscopy and fluorescence probing show that stalks primarily contain carboxyl-rich polysaccharides. On the basis of these results, we suggest a physiological model for Fe oxidation in which cells excrete oxidized Fe bound to organic polymers. These organic molecules retard mineral growth, preventing cell encrustation. This model describes an essential role for stalk formation in FeOB growth. We suggest that stalk-like morphologies observed in modern and ancient samples may be correlated confidently with the Fe-oxidizing metabolism as a robust biosignature.

Chan, Clara S; Fakra, Sirine C; Emerson, David; Fleming, Emily J; Edwards, Katrina J

2011-07-01T23:59:59.000Z

340

The effects of petroleum residues and the associated degrading bacteria on the development and survival of the larvae of Penaeus aztecus ives  

E-Print Network [OSTI]

THE EFFECTS OF PETROLEUM RESIDUES AND THE ASSOCIATED DEGRADING BACTERIA ON THE DEVELOPMENT AND SURVIVAL OF THE LARVAE OF PENAEUS AZTECUS IVES A Thesis by JOHN CURTIS GLARY III Submitted to the Graduate College of Texas A&M University... in partial fulfillment of the requirement for the degree of MASTER OF SCIENCE December 1983 Major Subject: Biology THE EFFECTS OF PETROLEUM RESIDUES AND THE ASSOCIATED DEGRADING BACTERIA ON THE DEVELOPMENT AND SURVIVAL OF THE LARVAE OF PENAEUS AZTECUS...

Clary, John Curtis

1983-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


341

Overcoming the anaerobic hurdle in phenotypic microarrays: Generation andvisualization of growth curve data for Desulfovibrio vulgaris Hildenborough  

SciTech Connect (OSTI)

Growing anaerobic microorganisms in phenotypic microarrays (PM) and 96-well microtiter plates is an emerging technology that allows high throughput survey of the growth and physiology and/or phenotype of cultivable microorganisms. For non-model bacteria, a method for phenotypic analysis is invaluable, not only to serve as a starting point for further evaluation, but also to provide a broad understanding of the physiology of an uncharacterized wild-type organism or the physiology/phenotype of a newly created mutant of that organism. Given recent advances in genetic characterization and targeted mutations to elucidate genetic networks and metabolic pathways, high-throughput methods for determining phenotypic differences are essential. Here we outline challenges presented in studying the physiology and phenotype of a sulfate reducing anaerobic delta proteobacterium, Desulfovibrio vulgaris Hildenborough. Modifications of the commercially available OmniLog(TM) system (Hayward, CA) for experimental setup, and configuration, as well as considerations in PM data analysis are presented. Also highlighted here is data viewing software that enables users to view and compare multiple PM data sets. The PM method promises to be a valuable strategy in our systems biology approach to D. vulgaris studies and is readily applicable to other anaerobic and aerobic bacteria.

Borglin, Sharon E; Joyner, Dominique; Jacobsen, Janet; Mukhopadhyay, Aindrila; Hazen, Terry C.

2008-10-04T23:59:59.000Z

342

Use of bromodeoxyuridine immunocapture to identify psychrotolerant phenanthrene-degrading bacteria in phenanthrene-enriched polluted Baltic Sea sediments  

SciTech Connect (OSTI)

The aim of this study was to enrich and identify psychrotolerant phenanthrenedegrading bacteria from polluted Baltic Sea sediments. Polyaromatic hydrocarbon (PAH)-contaminated sediments were spiked with phenanthrene and incubated for 2 months in the presence of bromodeoxyuridine that is incorporated into the DNA of replicating cells. The bromodeoxyuridine-incorporated DNA was extracted by immunocapture and analyzed by terminal-restriction fragment length polymorphism and 16S rRNA gene cloning and sequencing to identify bacterial populations that were growing. In addition, degradation genes were quantified in the bromodeoxyuridine-incorporated DNA by real-time PCR. Phenanthrene concentrations decreased after 2 months of incubation in the phenanthrene-enriched sediments and this reduction correlated to increases in copy numbers of xylE and phnAc dioxygenase genes. Representatives of Exiguobacterium, Schewanella,Methylomonas, Pseudomonas, Bacteroides and an uncultured Deltaproteobacterium and a Gammaproteobacterium dominated the growing community in the phenanthrene spiked sediments. Isolates that were closely related to three of these bacteria (two pseudomonads and an Exiguobacterium sp.) could reduce phenanthrene concentrations in pure cultures and they all harbored phnAc dioxygenase genes. These results confirm that this combination of culture-based and molecular approaches was useful for identification of actively growing bacterial species with a high potential for phenanthrene degradation.

Edlund, A.; Jansson, J.

2008-05-01T23:59:59.000Z

343

E-Print Network 3.0 - active biopolymer network Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

in the blood flow, active swimming of microorganisms (e.g. sperm, bacteria ... Source: Frey, Erwin - Arnold Sommerfeld Center for Theoretical Physics, Ludwig-Maximilians-Univers...

344

Aerobic Biodegradation of N-Nitrosodimethylamine (NDMA)  

E-Print Network [OSTI]

DHS, 2002; US EPA, 2001). Its presence in the environment has been linked to aerospace facilities generally to the discharge of water and wastewater disinfected with chlorine (Cal DHS, 2002; Mitch et al

Wood, Thomas K.

345

Processing of cellulosic material by a cellulase-containing cell-free fermentate produced from cellulase-producing bacteria, ATCC 55702  

DOE Patents [OSTI]

Bacteria which produce large amounts of a cellulase-containing cell-free fermentate, have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase degrading bacterium ATCC 55702, which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic materials.

Dees, H. Craig (Lenoir City, TN)

1998-01-01T23:59:59.000Z

346

Twenty-One Genome Sequences from Pseudomonas Species and 19 Genome Sequences from Diverse Bacteria Isolated from the Rhizosphere and Endosphere of Populus deltoides  

SciTech Connect (OSTI)

To aid in the investigation of the Populus deltoides microbiome we generated draft genome sequences for twenty one Pseudomonas and twenty one other diverse bacteria isolated from Populus deltoides roots. Genome sequences for isolates similar to Acidovorax, Bradyrhizobium, Brevibacillus, Burkholderia, Caulobacter, Chryseobacterium, Flavobacterium, Herbaspirillum, Novosphingobium, Pantoea, Phyllobacterium, Polaromonas, Rhizobium, Sphingobium and Variovorax were generated.

Brown, Steven D [ORNL; Utturkar, Sagar M [ORNL; Klingeman, Dawn Marie [ORNL; Johnson, Courtney M [ORNL; Martin, Stanton [ORNL; Land, Miriam L [ORNL; Lu, Tse-Yuan [ORNL; Schadt, Christopher Warren [ORNL; Doktycz, Mitchel John [ORNL; Pelletier, Dale A [ORNL

2012-01-01T23:59:59.000Z

347

USATODAY.com -Bacteria-powered battery runs on a sweet tooth Page 1 Close|EMAIL THIS|SAVE THIS  

E-Print Network [OSTI]

|SAVE THIS Bacteria-powered battery runs on a sweet tooth By Elizabeth Weise USA TODAY Scientists at the University in carbohydrates and turn them into electricity. Previous research has shown it is possible to use microbes to turn organic matter into electricity, but the process required the use of added materials to shuttle

Lovley, Derek

348

A soil microscale study to reveal the heterogeneity of Hg(II) impact on indigenous bacteria by quantication of adapted phenotypes and  

E-Print Network [OSTI]

toxicants. Heavy metals in soil are known to have a deleterious e¡ect on the num- bers of bacteria; RISA; Indirect enumeration 1. Introduction Bacterial response to heavy metal contamination in soil,2]). Several studies reported an impact of heavy metals at the community level using phenotypic or genetic

Thioulouse, Jean

349

Making more efficient fuel cells 08.09.2009 -Bacteria that generate significant amounts of electricity could be used in microbial fuel cells to provide  

E-Print Network [OSTI]

of electricity could be used in microbial fuel cells to provide power in remote environments or to convert waste to electricity. Professor Derek Lovley from the University of Massachusetts, USA isolated bacteria with large. The researchers isolated a strain of Geobacter sulfurreducens which they called KN400 that grew prolifically

Lovley, Derek

350

Bacteria that generate significant amounts of electricity could be used in microbial fuel cells to provide power in remote environments or to convert  

E-Print Network [OSTI]

Bacteria that generate significant amounts of electricity could be used in microbial fuel cells to provide power in remote environments or to convert waste to electricity. Professor Derek Lovley from at Heriot-Watt University, Edinburgh. The researchers isolated a strain of Geobacter sulfurreducens which

Lovley, Derek

351

Rust-Breathing Bacteria: Miracle Microbes? Search news.nationalgeographic.com Sign up for our free e-mail newsletter Also see: Today's Top Stories  

E-Print Network [OSTI]

colleagues sequenced the genome of a Geobacter species that can generate electricity and cleanup groundwater up polluted groundwater, generate electricity, and may harbor clues to the origins of life. That with another wonder from the Geobacter genus of bacteria. "When we think we have hit the last of the big

Lovley, Derek

352

Bacteria power may be possible http://www.masslive.com/hampfrank/republican/index.ssf?/base/news... 1 of 2 5/12/2006 2:33 PM  

E-Print Network [OSTI]

fuel cells powered by so-called geobacter, tiny bacteria that convert iron to electricity, to the point geobacter) to change that trash to electricity," Lovley said. A typical fuel cell converts fuels that conduct electricity to the outside for use as power. The UMass researchers found that when the geobacter

Lovley, Derek

353

Pentanol isomer synthesis in engineered microorganisms  

E-Print Network [OSTI]

potential application as biofuels. They are found as natural3-Methyl-1-butanol . Biofuels 32 MJ/L for gasoline) and aabout microbial production of biofuels in general have been

Cann, Anthony F.; Liao, James C.

2010-01-01T23:59:59.000Z

354

Microorganism genomics, compositions and methods related thereto  

DOE Patents [OSTI]

The present invention provides methods and compositions for accessing, in a generally unbaised manner, a diverse genetic pool for genes involved in biosynthetic pathways. The invention also provides compounds which can be identified by cloning biosynthetic pathways.

Handelsman, Jo (Madison, WI); Goodman, Robert M. (Madison, WI); Rondon, Michelle R. (Madison, WI)

2001-01-01T23:59:59.000Z

355

METHANE INCORPORATION BY PROCARYOTIC PHOTOSYNTHETIC MICROORGANISMS  

E-Print Network [OSTI]

blue-green alga. Low-density nuclear material is abundant inobtained from the New England Nuclear Corpor- ation (NEN) inin C H The New England Nuclear Corporation was unable to

Norton, Charles J.; Kirk, Martha; Calvin, Melvin

1970-01-01T23:59:59.000Z

356

Search for ancient microorganisms in Lake Baikal  

SciTech Connect (OSTI)

Lake Baikal in Russia, the world's oldest and deepest continental lake lies in south central Siberia, near the border to Mongolia. The lake is 1,643 m deep and has an area of about 46,000 km2. It holds one-fifth of all the terrestrial fresh water on Earth. Lake Baikal occupies the deepest portion of the Baikal Rift Zone. It was formed some 30-45 million years ago. The isolated Lake Baikal ecosystem represents a unique niche in nature based on its historical formation. The microbial diversity present in this environment has not yet been fully harvested or examined for products and processes of commercial interest and value. Thus, the collection of water, soil, and sub-bottom sediment samples was decided to characterize the microbial diversity of the isolated strains and to screen the isolates for their biotechnological value.

Hunter-Cevera, Jennie C.; Repin, Vladimir E.; Torok, Tamas

2000-06-14T23:59:59.000Z

357

Single-cell analysis of growth in budding yeast and bacteria reveals a common size regulation strategy  

E-Print Network [OSTI]

Unicellular organism from various kingdoms of life face the challenge of regulating their size. Despite decades of research, we still do not have a good understanding of the molecular mechanisms involved in this regulation, and how cells coordinate the different events of the cell cycle, such as growth, division and DNA replication is still unclear. Here, we report on experimental results for the budding yeast Saccharomyces cerevisiae and the bacterium Escherichia coli, showing that, remarkably, they share a common strategy for cell size control. We collected data on single-cell growth and cell cycle progression in S. cerevisiae in several growth media and estimated the distributions of size at birth and interdivision time as well as their correlations throughout cell lineages. We also performed the same analysis on previously collected data on single-cell growth and division in E. coli. The results are in quantitative agreement with the predictions of the incremental model, which leads to the addition of a constant volume (up to fluctuations), independent of size at birth, between birth and division; we show that in both organisms size at birth and size at division exhibit a linear relationship with slope one. This result, together with extended additional analysis supporting the incremental model, argues against the existing "critical size" paradigm for cell size control in bacteria and yeast.

Ilya Soifer; Lydia Robert; Naama Barkai; Ariel Amir

2014-10-23T23:59:59.000Z

358

Phase Preference by Active, Acetate-Utilizing Bacteria at the Rifle, CO Integrated Field Research Challenge Site  

SciTech Connect (OSTI)

Previous experiments at the Rifle, Colorado Integrated Field Research Challenge (IFRC) site demonstrated that field-scale addition of acetate to groundwater reduced the ambient soluble uranium concentration. In this report, sediment samples collected before and after acetate field addition were used to assess the active microbes via {sup 13}C acetate stable isotope probing on 3 phases [coarse sand, fines (8-approximately 150 {micro}m), groundwater (0.2-8 {micro}m)] over a 24-day time frame. TRFLP results generally indicated a stronger signal in {sup 13}C-DNA in the 'fines' fraction compared to the sand and groundwater. Before the field-scale acetate addition, a Geobacter-like group primarily synthesized {sup 13}C-DNA in the groundwater phase, an alpha Proteobacterium primarily grew on the fines/sands, and an Acinetobacter sp. and Decholoromonas-like OTU utilized much of the {sup 13}C acetate in both groundwater and particle-associated phases. At the termination of the field-scale acetate addition, the Geobacter-like species was active on the solid phases rather than the groundwater, while the other bacterial groups had very reduced newly synthesized DNA signal. These findings will help to delineate the acetate utilization patterns of bacteria in the field and can lead to improved methods for stimulating distinct microbial populations in situ.

Kerkhof, L.; Williams, K.H.; Long, P.E.; McGuinness, L.

2011-02-21T23:59:59.000Z

359

Single-cell genomics reveal metabolic strategies for microbial growth and survival in an oligotrophic aquifer  

SciTech Connect (OSTI)

Bacteria from the genus Pedobacter are a major component of microbial assemblages at Hanford Site and have been shown to significantly change in abundance in response to the subsurface intrusion of Columbia River water. Here we employed single cell genomics techniques to shed light on the physiological niche of these microorganisms. Analysis of four Pedobacter single amplified genomes (SAGs) from Hanford Site sediments revealed a chemoheterotrophic lifestyle, with the potential to exist under both aerobic and microaerophilic conditions via expression of both aa3­?type and cbb3-type cytochrome c oxidases. These SAGs encoded a wide-range of both intra-and extra­-cellular carbohydrate-active enzymes, potentially enabling the degradation of recalcitrant substrates such as xylan and chitin, and the utilization of more labile sugars such as mannose and fucose. Coupled to these enzymes, a diversity of transporters and sugar-binding molecules were involved in the uptake of carbon from the extracellular local environment. The SAGs were enriched in TonB-dependent receptors (TBDRs), which play a key role in uptake of substrates resulting from degradation of recalcitrant carbon. CRISPR-Cas mechanisms for resisting viral infections were identified in all SAGs. These data demonstrate the potential mechanisms utilized for persistence by heterotrophic microorganisms in a carbon-limited aquifer, and hint at potential linkages between observed Pedobacter abundance shifts within the 300 Area subsurface and biogeochemical shifts associated with Columbia River water intrusion.

Wilkins, Michael J.; Kennedy, David W.; Castelle, Cindy; Field, Erin; Stepanauskas, Ramunas; Fredrickson, Jim K.; Konopka, Allan

2014-02-01T23:59:59.000Z

360

Competitive Reduction of Pertechnetate (99TcO4?) by Dissimilatory Metal Reducing Bacteria and Biogenic Fe(II)  

SciTech Connect (OSTI)

The fate of pertechnetate (99Tc(VII)O4 -) during bioreduction was investigated in the presence of 2-line ferrihydrite (Fh) and various dissimilatory metal reducing bacteria (DMRB) (Geobacter, Anaeromyxobacter, Shewanella) in comparison with TcO4 - bioreduction in the absence of Fh. In the presence of Fh, Tc was present primarily as a fine-grained Tc(IV)/Fe precipitate that was distinct from the Tc(IV)O2 ·nH2O solids produced by direct biological Tc(VII) reduction. Aqueous Tc concentrations (<0.2 ?m) in the bioreduced Fh suspensions (1.7 to 3.2 × 10-9 mol L-1) were over 1 order of magnitude lower than when TcO4 - was biologically reduced in the absence of Fh (4.0 × 10-8 to 1.0 × 10-7 mol L-1). EXAFS analyses of the bioreduced Fh-Tc products were consistent with variable chain length Tc-O octahedra bonded to Fe-O octahedra associated with the surface of the residual or secondary Fe(III) oxide. In contrast, biogenic TcO2 ·nH2O had significantly more Tc-Tc second neighbors and a distinct long-range order consistent with small particle polymers of TcO2. In Fe-rich subsurface sediments, the reduction of Tc(VII) by Fe(II) may predominate over direct microbial pathways, potentially leading to lower concentrations of aqueous 99Tc(IV).

Plymale, Andrew E.; Fredrickson, Jim K.; Zachara, John M.; Dohnalkova, Alice; Heald, Steve M.; Moore, Dean A.; Kennedy, David W.; Marshall, Matthew J.; Wang, Chong M.; Resch, Charles T.; Nachimuthu, Ponnusamy

2011-02-01T23:59:59.000Z

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


361

CORROSION SCIENCE SECTION CORROSION--Vol. 58, No. 5 417  

E-Print Network [OSTI]

CORROSION SCIENCE SECTION CORROSION--Vol. 58, No. 5 417 0010-9312/02/000095/$5.00+$0.50/0 © 2002 of California­Irvine, Irvine, CA 92697-2575. Corrosion Control of Mild Steel by Aerobic Bacteria Under.J. Arps, and J.C. Earthman****** ABSTRACT Corrosion of mild steel under aerobic conditions in the pres

Wood, Thomas K.

362

Significant Association between Sulfate-Reducing Bacteria and Uranium-Reducing Microbial Communities as Revealed by a Combined Massively Parallel Sequencing-Indicator Species Approach  

SciTech Connect (OSTI)

Massively parallel sequencing has provided a more affordable and high-throughput method to study microbial communities, although it has mostly been used in an exploratory fashion. We combined pyrosequencing with a strict indicator species statistical analysis to test if bacteria specifically responded to ethanol injection that successfully promoted dissimilatory uranium(VI) reduction in the subsurface of a uranium contamination plume at the Oak Ridge Field Research Center in Tennessee. Remediation was achieved with a hydraulic flow control consisting of an inner loop, where ethanol was injected, and an outer loop for flow-field protection. This strategy reduced uranium concentrations in groundwater to levels below 0.126 M and created geochemical gradients in electron donors from the inner-loop injection well toward the outer loop and downgradient flow path. Our analysis with 15 sediment samples from the entire test area found significant indicator species that showed a high degree of adaptation to the three different hydrochemical-created conditions. Castellaniella and Rhodanobacter characterized areas with low pH, heavy metals, and low bioactivity, while sulfate-, Fe(III)-, and U(VI)-reducing bacteria (Desulfovibrio, Anaeromyxobacter, and Desulfosporosinus) were indicators of areas where U(VI) reduction occurred. The abundance of these bacteria, as well as the Fe(III) and U(VI) reducer Geobacter, correlated with the hydraulic connectivity to the substrate injection site, suggesting that the selected populations were a direct response to electron donor addition by the groundwater flow path. A false-discovery-rate approach was implemented to discard false-positive results by chance, given the large amount of data compared.

Cardenas, Erick [Michigan State University, East Lansing; Leigh, Mary Beth [Michigan State University, East Lansing; Marsh, Terence [Michigan State University, East Lansing; Tiedje, James M. [Michigan State University, East Lansing; Wu, Wei-min [Stanford University; Luo, Jian [Stanford University; Ginder-Vogel, Matthew [Stanford University; Kitanidis, Peter K. [Stanford University; Criddle, Craig [Stanford University; Carley, Jack M [ORNL; Carroll, Sue L [ORNL; Gentry, Terry J [ORNL; Watson, David B [ORNL; Gu, Baohua [ORNL; Jardine, Philip M [ORNL; Zhou, Jizhong [ORNL

2010-10-01T23:59:59.000Z

363

A combined massively parallel sequencing indicator species approach revealed significant association between sulfate-reducing bacteria and uranium-reducing microbial communities  

SciTech Connect (OSTI)

Massively parallel sequencing has provided a more affordable and high throughput method to study microbial communities, although it has been mostly used in an exploratory fashion. We combined pyrosequencing with a strict indicator species statistical analysis to test if bacteria specifically responded to ethanol injection that successfully promoted dissimilatory uranium (VI) reduction in the subsurface of a uranium contamination plume at the Oak Ridge Field Research Center in Tennessee, USA. Remediation was achieved with a hydraulic flow control consisting of an inner loop, where ethanol was injected, and an outer loop for flow field protection. This strategy reduced uranium concentrations in groundwater to levels below 0.126 {micro}M, and created geochemical gradients in electron donors from the inner loop injection well towards the outer loop and down-gradient flow path. Our analysis with 15 sediment samples from the entire test area found significant indicator species that showed a high degree of adaptation to the three different hydrochemical created conditions. Castellaniella, and Rhodanobacter characterized areas with low pH, heavy metals, and low bioactivity; while sulfate-, Fe(III)-, and U(VI)-reducing bacteria (Desulfovibrio, Anaeromyxobacter, and Desulfosporosinus) were indicators of areas where U(VI) reduction occurred. Abundance of these bacteria as well as the Fe(III)- and U(VI)-reducer Geobacter correlated with the hydraulic connectivity to the substrate injection site, suggesting that the selected populations were a direct response to the electron donor addition and by the groundwater flow path. A false discovery rate approach was implemented to discard false positives by chance given the large amount of data compared.

Cardenas, Erick [Michigan State University, East Lansing; Wu, Wei-min [Stanford University; Leigh, Mary Beth [Michigan State University, East Lansing; Carley, Jack M [ORNL; Carroll, Sue L [ORNL; Gentry, Terry [Texas A& M University; Luo, Jian [Georgia Institute of Technology; Watson, David B [ORNL; Gu, Baohua [ORNL; Ginder-Vogel, Matthew A. [Stanford University; Kitanidis, Peter K. [Stanford University; Jardine, Philip [University of Tennessee; Kelly, Shelly D [Argonne National Laboratory (ANL); Zhou, Jizhong [University of Oklahoma, Norman; Criddle, Craig [Stanford University; Marsh, Terence [Michigan State University, East Lansing; Tiedje, James [Michigan State University, East Lansing

2010-08-01T23:59:59.000Z

364

Chromium(VI) bioremoval by pseudomonas bacteria: role of microbial exudates for natural attenuation and biotreatment of Cr(VI) contamination  

SciTech Connect (OSTI)

Laboratory batch and column experiments were conducted to investigate the role of microbial exudates, e.g., exopolymeric substance (EPS) and alginic acid, on microbial Cr(VI) reduction by two different Pseudomonas strains (P. putida P18 and P. aeuroginosa P16) as a method for treating subsurface environment contaminated with Cr(VI). Our results indicate that microbial exudates significantly enhanced microbial Cr(VI) reduction rates by forming less toxic and highly soluble organo-Cr(III) complexes despite the fact Cr(III) has a very low solubility under the experimental conditions studied (e.g., pH 7). The formation of soluble organo-Cr(III) complexes led to the protection of the cells and chromate reductases from inactivation. In systems with no organic ligands, soluble organo-Cr(III) end products were formed between Cr(III) and the EPS directly released by bacteria due to cell lysis. Our results also provide evidence that cell lysis played an important role in microbial Cr(VI) reduction by Pseudomonas bacteria due to the release of constitutive reductases that intracellularly and/or extracellularly catalyzed the reduction of Cr(VI) to Cr(III). The overall results highlight the need for incorporation of the release and formation of organo-Cr(III) complexes into reactive transport models to more accurately design and monitor in situ microbial remediation techniques for the treatment of subsurface systems contaminated with Cr(VI).

Dogan, N.M.; Dodge, C.; Kantar, C.; Gulcan, S.; Yilmaz, B.C.; Mazmanci, M.A.

2011-02-14T23:59:59.000Z

365

Chromium(VI) Bioremoval by Pseudomonas Bacteria: Role of Microbial Exudates for Natural Attenuation and Biotreatment of Cr(VI) Contamination  

SciTech Connect (OSTI)

Laboratory batch and column experiments were conducted to investigate the role of microbial exudates, e.g., exopolymeric substance (EPS) and alginic acid, on microbial Cr(VI) reduction by two different Pseudomonas strains (P. putida P18 and P. aeuroginosa P16) as a method for treating subsurface environment contaminated with Cr(VI). Our results indicate that microbial exudates significantly enhanced microbial Cr(VI) reduction rates by forming less toxic and highly soluble organo-Cr(III) complexes despite the fact Cr(III) has a very low solubility under the experimental conditions studied (e.g., pH 7). The formation of soluble organo-Cr(III) complexes led to the protection of the cells and chromate reductases from inactivation. In systems with no organic ligands, soluble organo-Cr(III) end products were formed between Cr(III) and the EPS directly released by bacteria due to cell lysis. Our results also provide evidence that cell lysis played an important role in microbial Cr(VI) reduction by Pseudomonas bacteria due to the release of constitutive reductases that intracellularly and/or extracellularly catalyzed the reduction of Cr(VI) to Cr(III). The overall results highlight the need for incorporation of the release and formation of organo-Cr(III) complexes into reactive transport models to more accurately design and monitor in situ microbial remediation techniques for the treatment of subsurface systems contaminated with Cr(VI).

N Mercan Dogan; C Kantar; S Gulcan; C Dodge; B Coskun Yilmaz; M Ali Mazmanci

2011-12-31T23:59:59.000Z

366

Use of the high-energy x-ray microprobe at the Advanced Photon Source to investigate the interactions between metals and bacteria.  

SciTech Connect (OSTI)

Understanding the fate of heavy-metal contaminants in the environment is of fundamental importance in the development and evaluation of effective remediation and sequestration strategies. Among the factors influencing the transport of these contaminants are their chemical separation and the chemical and physical attributes of the surrounding medium. Bacteria and the extracellular material associated with them are thought to play a key role in determining a contaminant's speciation and thus its mobility in the environment. In addition, the microenvironment at and adjacent to actively metabolizing cell surfaces can be significantly different from the bulk environment. Thus, the spatial distribution and chemical separation of contaminants and elements that are key to biological processes must be characterized at micron and submicron resolution in order to understand the microscopic physical, geological, chemical, and biological interfaces that determine a contaminant's macroscopic fate. Hard X-ray microimaging is a powerful technique for the element-specific investigation of complex environmental samples at th needed micron and submicron resolution. An important advantage of this technique results from the large penetration depth of hard X-rays in water. This advantage minimizes the requirements for sample preparation and allows the detailed study of hydrated samples. This paper presents results of studies of the spatial distribution of naturally occurring metals and a heavy-metal contaminant (Cr) in and near hydrated bacteria (Pseudomonas fluorescens) in the early stages of biofilm development, performed at the Advanced Photon Source Sector 2 X-ray microscopy beamline.

Kemner, K. M.; Lai, B.; Maser, J.; Schneegurt, M. A.; Cai, Z.; Ilinski, P. P.; Kulpa, C. F.; Legnini, D. G.; Nealson, K. H.; Pratt, S. T.; Rodrigues, W.; Tischler, M. L.; Yun, W.

1999-09-30T23:59:59.000Z

367

Assessing the Role of Iron Sulfides in the Long Term Sequestration of Uranium by Sulfate-Reducing Bacteria  

SciTech Connect (OSTI)

This overarching aim of this project was to identify the role of biogenic and synthetic iron-sulfide minerals in the long-term sequestration of reduced U(IV) formed under sulfate-reducing conditions when subjected to re-oxidizing conditions. The work reported herein was achieved through the collaborative research effort conducted at Arizona State University (ASU) and the University of Michigan (UM). Research at ASU, focused on the biogenesis aspects, examined the biogeochemical bases for iron-sulfide production by Desulfovibrio vulgaris, a Gram-negative bacterium that is one of the most-studied strains of sulfate-reducing bacteria. A series of experimental studies were performed to investigate comprehensively important metabolic and environmental factors that affect the rates of sulfate reduction and iron-sulfide precipitation, the mineralogical characteristics of the iron sulfides, and how uranium is reduced or co-reduced by D. vulagaris. FeS production studies revealed that controlling the pH affected the growth of D. vulgaris and strongly influenced the formation and growth of FeS solids. In particular, lower pH produced larger-sized mackinawite (Fe1+xS). Greater accumulation of free sulfide, from more sulfate reduction by D. vulgaris, also led to larger-sized mackinawite and stimulated mackinawite transformation to greigite (Fe3S4) when the free sulfide concentration was 29.3 mM. On the other hand, using solid Fe(III) (hydr)oxides as the iron source led to less productivity of FeS due to their slow and incomplete dissolution and scavenging of sulfide. Furthermore, sufficient free Fe2+, particularly during Fe(III) (hydr)oxide reductions, led to the additional formation of vivianite [Fe3(PO4)2•8(H2O)]. The U(VI) reduction studies revealed that D. vulgaris reduced U(VI) fastest when accumulating sulfide from concomitant sulfate reduction, since direct enzymatic and sulfide-based reductions of U(VI) occurred in parallel. The UO2 produced in presence of ferrous iron was poorly crystalline. At UM, laboratory-scale reactor studies were performed to assess the potential for the predominant abiotic reductants formed under sulfate reducing conditions (SRCs) to: (1) reduce U(VI) in contaminated groundwater sediments), and (2) inhibit the re-oxidation of U(IV) species, and in particular, uraninite (UO2(s)). Under SRCs, mackinawite and aqueous sulfide are the key reductants expected to form. To assess their potential for abiotic reduction of U(VI) species, a series of experiments were performed in which either FeS or S(-II) was added to solutions of U(VI), with the rates of conversion to U(IV) solids monitored as a function of pH, and carbonate and calcium concentration. In the presence of FeS and absence of oxygen or carbonate, U(IV) was completely reduced uraninite. S(-II) was also found to be an effective reductant of aqueous phase U(VI) species and produced uraninite, with the kinetics and extent of reduction depending on geochemical conditions. U(VI) reduction to uraninite was faster under higher S(-II) concentrations but was slowed by an increase in the dissolved Ca or carbonate concentration. Rapid reduction of U(VI) occurred at circumneutral pH but virtually no reduction occurred at pH 10.7. In general, dissolved Ca and carbonate slowed abiotic U(VI) reduction by forming stable Ca-U(VI)-carbonate soluble complexes that are resistant to reaction with aqueous sulfide. To investigate the stability of U(IV) against re-oxidation in the presence of iron sulfides by oxidants in simulated groundwater environments, and to develop a mechanistic understanding the controlling redox processes, continuously-mixed batch reactor (CMBR) and flow-through reactor (CMFR) studies were performed at UM. In these studies a series of experiments were conducted under various oxic groundwater conditions to examine the effectiveness of FeS as an oxygen scavenger to retard UO2 dissolution. The results indicate that FeS is an effective oxygen scavenger, and can lower the rate of oxidative dissolution of UO2 by over an order of magnitude compared to

Hayes, Kim F.; Bi, Yuqiang; Carpenter, Julian; Hyng, Sung Pil; Rittmann, Bruce E.; Zhou, Chen; Vannela, Raveender; Davis, James A.

2014-01-01T23:59:59.000Z

368

UPI.com: Bacteria might prove to be energy source http://license.icopyright.net/user/viewFreeUse.act?fuid=Mzk4NDQ= 1 of 1 6/5/2006 12:31 PM  

E-Print Network [OSTI]

fuel cells show promise for conversion of organic wastes and renewable biomass to electricity by allowing bacteria in microbial fuel cells to grow on biofilms in the electrodes of a fuel cell. This week, but further optimization is required for most applications," said Derek Lovley of the University

Lovley, Derek

369

23/01/2008 20:56Null Hypothesis | Straight Talking Page 1 of 2http://www.null-hypothesis.co.uk/science/straight-talking/item/selfish_bacteria_threaten_slime_cities  

E-Print Network [OSTI]

and even cheat on each other," Diggle said. Sound like anyone you know? As Diggle hints, such human Experiment: Speed of Light Candiru: The Willy Fish For The Love Of PCR Video Games & Sick Leave 10 Rules.com Liquid Zeolite 100% Pure Zeolite 30ml bottles Lab- Certified 100% Bacteria-Free. www.liquid

West, Stuart

370

Effects of diesel exhaust on the microbiota within a tuffaceous tunnel system  

SciTech Connect (OSTI)

The abundance and distribution of microbiota that may be impacted by diesel and diesel exhaust were investigated from three depths into the walls and invert (floor) of U12n tunnel at Rainier Mesa, Nevada Test Site, a potential geological analog of Yucca Mountain. Enumerations included total cell counts, and numbers of aerobic heterotrophic, sulfate-reducing, nitrate-reducing, and diesel-degrading bacteria. Additionally, the disappearance of total petroleum hydrocarbons was determined in microcosms containing subsurface materials that were amended with diesel fuel. Results revealed that microbes capable of utilizing diesel and diesel combustion products were present in the subsurface in both the walls and the invert of the tunnel. The abundance of specific bacterial types in the tunnel invert, a perturbed environment, was greater than that observed in the tunnel wall. Few trends of microbial distribution either into the tunnel wall or the invert were noted with the exception of aerobic heterotrophic abundance which increased with depth into the wall and decreased with depth into the invert. No correlation between microbiota and a specific introduced chemical species have yet been determined. The potential for microbial contamination of the tunnel wall during sampling was determined to be negligible by the use of fluorescently labeled latex spheres (1{mu}m in dia.) as tracers. Results indicate that additional investigations might be needed to examine the microbiota and their possible impacts on the geology and geochemistry of the subsurface, both indigenous microbiota and those microorganisms that will likely be introduced by anthropogenic activity associated with the construction of a high-level waste repository.

Haldeman, D.L.; Lagadinos, T.; Amy, P.S. [Univ. of Nevada, Las Vegas, NV (United States); Hersman, L. [Los Alamos National Lab., NM (United States); Meike, A. [Lawrence Livermore National Lab., Livermore, CA (United States)

1996-08-01T23:59:59.000Z

371

Impact of EPS on Digestion of Waste Activate Sludge Thomas Gostanian  

E-Print Network [OSTI]

Impact of EPS on Digestion of Waste Activate Sludge Thomas Gostanian Faculty Mentor: Professor Chul Park, Civil & Environmental Engineering Digestion by bacteria is a widely used method for organic is by either aerobic or anaerobic self-digestion, in which the bacteria consume their own mass. Currently

Mountziaris, T. J.

372

Molecular analysis of deep subsurface Cretaceous rock indicates abundant Fe(III)- and S°-reducing bacteria in a sulfate-rich environment  

SciTech Connect (OSTI)

A multi-level sampler (MLS) was emplaced in a borehole straddling anaerobic, sulfate-rich Cretaceous-era shale and sandstone rock formations {approx}200 m below ground surface at Cerro Negro, New Mexico. Sterile quartzite sand contained in chambers in the sampler allowed in situ colonization and recovery of nucleic acids for molecular analyses. DGGE and 16S rRNA gene cloning results indicated a homogeneously distributed bacterial community across the shale/sandstone interface. ?-Proteobacteria sequences were common at all depths, and were dominated by members of the Geobacteraceae family (Pelobacter, Desulfuromonas, and Geobacter). Other members of this group are capable of dissimilatory Fe(III) and/or S0 reduction, but not sulfate reduction. RNA hybridization data also suggested that Fe(III)/S0 reducing bacteria were predominant. These findings are striking considering the lack of significant concentrations of these electron acceptors in this environment. The next most abundant bacterial group indicated was the sulfate reducers, including Desulfobacterium, Desulfocapsa and Desulfobulbus. Sequences related to fermenters, denitrifiers and acetogens were also recovered. The presence of a phylogenetically and functionally diverse microbial community in this deep subsurface environment likely reflects the complex nature of the primary energy and carbon sources, kerogen associated with the shale.

Kovacik, William P.; Takai, Ken; Mormile, Melanie R.; McKinley, James P.; Brockman, Fred J.; Fredrickson, Jim K.; Holben, William E.

2006-01-01T23:59:59.000Z

373

Test of electron beam technology on Savannah River Laboratory low-activity aqueous waste for destruction of benzene, benzene derivatives, and bacteria  

SciTech Connect (OSTI)

High energy radiation was studied as a means for destroying hazardous organic chemical wastes. Tests were conducted at bench scale with a {sup 60}Co source, and at full scale (387 l/min) with a 1.5 MV electron beam source. Bench scale tests for both benzene and phenol included 32 permutations of water quality factors. For some water qualities, as much as 99.99% of benzene or 90% of phenol were removed by 775 krads of {sup 60}Co irradiation. Full scale testing for destruction of benzene in a simulated waste-water mix showed loss of 97% of benzene following an 800 krad dose and 88% following a 500 krad dose. At these loss rates, approximately 5 Mrad of electron beam irradiation is required to reduce concentrations from 100 g/l to drinking water quality (5 {mu}g/l). Since many waste streams are also inhabited by bacterial populations which may affect filtering operations, the effect of irradiation on those populations was also studied. {sup 60}Co and electron beam irradiation were both lethal to the bacteria studied at irradiation levels far lower than were necessary to remove organic contaminants.

Dougal, R.A. [Univ. of South Carolina, Columbia, SC (United States). Dept. of Electrical and Computer Engineering

1993-08-01T23:59:59.000Z

374

Assessment of microbial processes on radionuclide mobility in shallow land burial. [West Valley, NY; Beatty, Nevada; Maxey Flats, Kentucky  

SciTech Connect (OSTI)

The impact of microbial metabolism of the organic substituents of low level radioactive wastes on radionuclide mobility in disposal sites, the nature of the microbial transformations involved in this metabolism and the effect of the prevailing environmental parameters on the quantities and types of metabolic intermediates accumulated were examined. Since both aerobic and anaerobic periods can occur during trench ecosystem development, oxidation capacities of the microbial community in the presence and absence of oxygen were analyzed. Results of gas studies performed at three commercial low level radioactive waste disposal sites were reviewed. Several deficiencies in available data were determined. Further research needs are suggested. This assessment has demonstrated that the biochemical capabilities expressed within the low level radioactive waste disposal site are common to a wide variety of soil bacteria. Hence, assuming trenches would not be placed in sites with such extreme abiotic conditions that all microbial activity is precluded, the microbial populations needed for colonization and decomposition of the organic waste substances are readily provided from the waste itself and from the soil of existing and any proposed disposal sites. Indeed, considering the ubiquity of occurrence of the microorganisms responsible for waste decomposition and the chemical nature of the organic waste material, long-term prevention of biodecomposition is difficult, if not impossible.

Colombo, P.; Tate, R.L. III; Weiss, A.J.

1982-07-01T23:59:59.000Z

375

Production and preliminary characterization of a recombinant triheme cytochrome c7 from Geobacter sulfurreducens in Escherichia coli.  

SciTech Connect (OSTI)

Multiheme cytochromes c have been found in a number of sulfate- and metal ion-reducing bacteria. Geobacter sulfurreducens is one of a family of microorganisms that oxidize organic compounds, with Fe(III) oxide as the terminal electron acceptor. A triheme 9.6 kDa cytochrome c{sub 7} from G. sulfurreducens is a part of the metal ion reduction pathway. We cloned the gene for cytochrome c{sub 7} and expressed it in Escherichia coli together with the cytochrome c maturation gene cluster, ccmABCDEFGH, on a separate plasmid. We designed two constructs, with and without an N-terminal His-tag. The untagged version provided a good yield (up to 6 mg/l of aerobic culture) of the fully matured protein, with all three hemes attached, while the N-terminal His-tag appeared to be detrimental for proper heme incorporation. The recombinant protein (untagged) is properly folded, it has the same molecular weight and displays the same absorption spectra, both in reduced and in oxidized forms, as the protein isolated from G. sulfurreducens and it is capable of reducing metal ions in vitro. The shape parameters for the recombinant cytochrome c{sub 7} determined by small angle X-ray scattering are in good agreement with the ones calculated from a homologous cytochrome c{sub 7} of known structure.

Londer, Y. Y.; Pokkuluri, P. R.; Tiede, D. M.; Schiffer, M.

2002-07-01T23:59:59.000Z

376

Microbial studies of compost: bacterial identification, and their potential for turfgrass pathogen suppression  

E-Print Network [OSTI]

Microbial studies of compost: bacterial identification, and their potential for turfgrass pathogen; accepted 17 April 2002 Keywords: Bacteria, compost, biocontrol disease suppression, grey snow mould Composting is the degradation of organic materials through the activities of diverse microorganisms

Boland, Greg J.

377

The Centre for Microbial Innovation (CMI) is a unique cross disciplinary environment for theoretical and practical research and  

E-Print Network [OSTI]

, health, energy and the environment. The CMI is harnessing the vast capabilities of microorganisms the presence of 12 undetermined genera and 30 known genera including 37 bacteria, 4 fungi and 1 microalga

Auckland, University of

378

GENETIC OPTIMIZATION FOR ALKALINE pH OF A CYANIDE DIHYDRATASE FROM PSEUDOMONAS STUTZERI  

E-Print Network [OSTI]

in the respiratory pathway. Surprisingly, several microorganisms can degrade and even survive cyanide’s presence. These microbes, fungi and bacteria, count on a variety of cyanide degrading enzymes that break cyanide into less toxic compounds. An example...

Rodriguez Mendoza, Alvaro

2011-08-08T23:59:59.000Z

379

Bioremediation of nanomaterials  

DOE Patents [OSTI]

The present invention provides a method comprising the use of microorganisms for nanotoxicity study and bioremediation. In some embodiment, the microorganisms are bacterial organisms such as Gram negative bacteria, which are used as model organisms to study the nanotoxicity of the fullerene compounds: E. coli W3110, a human related enterobacterium and Shewanella oneidensis MR-1, an environmentally important bacterium with versatile metabolism.

Chen, Frank Fanqing; Keasling, Jay D; Tang, Yinjie J

2013-05-14T23:59:59.000Z

380

Hydrol. Earth Syst. Sci., 11, 15811592, 2007 www.hydrol-earth-syst-sci.net/11/1581/2007/  

E-Print Network [OSTI]

for bathing and rowing activ- ities, as they were in the past. A model, appended to the hydro Polluted river waters can contain a large variety of pathogenic micro-organisms: viruses, bacteria and protozoa. The main origin of these micro-organisms is the direct and indirect release of men and warm

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


381

aerobic chinese hamster: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

backscatter coefficient quantitative estimates from Chinese hamster ovary cell pellet biophantoms Engineering Websites Summary: Ultrasonic backscatter coefficient...

382

aerobic methanotrophic communities: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and government. Forest Renewal BC funding- from stumpage fees and royalties that forest companies pay for the right to harvest timber on Crown lands- is reinvested in the forest,...

383

EFFECT OF AEROBIC EXERCISE INTERVENTION ON PAINFUL DIABETIC NEUROPATHY  

E-Print Network [OSTI]

to Linda D’Silva, Katherine Martin and Rupali Rupali, whom I have greatly enjoyed working with this year, for their exceptional contributions to the ENRGy2 project. I would like to thank Dr. Won Choi, Dr. Theresa Shireman, Dr. Dianne Durham, and Anita... the most out of life. Take charge of your diabetes. 26 Appendix C: Phone Screening Form PHONE SCREENING Exercise and Neuropathy Research Group version 2 (ENRGy2) Project Greetings: “We are conducting a study to see if exercise will improve nerve...

Yoo, Min

2013-05-31T23:59:59.000Z

384

activity aerobic capacity: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

of bovine and human endothelial cells that are inhibited by anti-FGF-2 antibody. Heat-inactivated FGF-2 retains its cell-adhesive activity despite its incapacity to bind...

385

Selective aerobic oxidation of hydrocarbons over supported gold catalysts.  

E-Print Network [OSTI]

??The selective oxidation of hydrocarbons is of vital importance for the production of valuable chemicals from crude oil and natural gas resources. Unfortunately, when using… (more)

Hereijgers, B.P.C.

2011-01-01T23:59:59.000Z

386

On-Site Wastewater Treatment Systems: Aerobic Treatment Unit (Spanish)  

E-Print Network [OSTI]

. Las personas que hagan la instalaci?n deben excavar un s?lo pozo con fondo nivelado, lo que reduce el tiempo de preparaci?n para la instalaci?n. El tanque de fibra de vidrio es lo suficientemente liviano como para que lo cargue una excavadora al lugar...

Lesikar, Bruce J.; Enciso, Juan

2000-08-29T23:59:59.000Z

387

aerobic room temperature: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

superconducting quantum interference device SQUID-temperature RT sample scanning superconduct- ing quantum interference device SQUID microscopy SSM is a very Weiss, Benjamin P....

388

SUPPLEMENTARY MATERIALS High current densities enable exoelectrogens to outcompete aerobic  

E-Print Network [OSTI]

, USA b State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 73, and the filled areas are that lost to oxygen leakage. The numbers above the stacked columns are the CE values. 0 for the measurement of oxygen mass transfer coefficient of air-cathode. #12;6 Oxygen transfer calculation In order

389

aerobic sludge digestion: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

of organic wastes) and digestion (anaerobic treatment of organic wastes combined with biogas production) are important waste management strategies with increasing significance...

390

aerobic nicotinic acid: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

g magnesium chloride, 3.24 g magnesium sulfate bromide, 34 mg strontium chloride, 22 mg boric acid, 4 mg sodium silicate, 2.4 mg sodium fluoride, 1.6 mg Bae, Jin-Woo 189 XYLARIC...

391

DNA UPTAKE BY TRANSFORMABLE BACTERIA  

SciTech Connect (OSTI)

The various processes of DNA uptake by cells can be categorized as: viral DNA entry, conjugation, or transformation. Within each category, a variety of mechanisms have been found. However, considerable similarities occur among the different mechanisms of conjugation and, especially, transformation. All of these natural mechanisms of DNA transfer are quite elaborate and involve multiple protein components, as the case may be, of the virus, the donor cell, and the recipient cell. The mechanisms of viral infection and conjugation will be discussed mainly with respect to their relevance to transformation.

LACKS,S.A.

1999-09-07T23:59:59.000Z

392

Sampling for Bacteria in Wells  

E-Print Network [OSTI]

This publication will instruct you on the proper procedures for collecting a sample from a water well for bacteriological analysis....

Lesikar, Bruce J.

2001-11-15T23:59:59.000Z

393

Bacteria Photosynthesis | ornl.gov  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth (AOD)ProductssondeadjustsondeadjustAboutScience ProgramBackground High Energy Physics (HEP) HEP Home'Low tech'

394

Bacteria to the Rescue | EMSL  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth (AOD)ProductssondeadjustsondeadjustAboutScience ProgramBackground High Energy Physics (HEP) HEP

395

HOST MICROBE INTERACTIONS Microorganisms Associated with Feathers of Barn Swallows  

E-Print Network [OSTI]

Contaminated Areas Around Chernobyl Gábor Árpád Czirják & Anders Pape Møller & Timothy A. Mousseau & Philipp Science+Business Media, LLC 2010 Abstract The Chernobyl catastrophe provides a rare opportunity to study migratory passerine, around Chernobyl in relation to levels of ground level environmental radiation. After

Mousseau, Timothy A.

396

Investigation of Interfacial Chemistry of Microorganisms Jani C. Ingram,1  

E-Print Network [OSTI]

Berkeley National Laboratory, CA 3 Shawnee High School, NJ INTRODUCTION Remediation of Department of Energy-Ying Holman,2 and Matthew Downing3 1 Idaho National Energy and Environmental Laboratory, ID 2 Lawrence Secondary ion mass spectrometry (SIMS) and synchrotron radiation Fourier transform infrared spectroscopy (SR

397

Combinatorial search strategies for the metabolic engineering of microorganisms  

E-Print Network [OSTI]

Although the field of microbial metabolic engineering has traditionally been dominated by rational and knowledge-driven approaches, recent advances in genetic engineering have led to the emergence of a new methodology based ...

Santos, Christine Nicole S. (Christine Nicole San Diego)

2010-01-01T23:59:59.000Z

398

Deciphering Active Estrogen-Degrading Microorganisms in Bioreactors  

E-Print Network [OSTI]

in biological wastewater treatment processes. This dissertation investigated factors affecting estrogen biodegradation in bioreactors. Specifically, research efforts were placed on characterization of several bacterial estrogen degraders (model strains...

Roh, Hyung Keun

2010-10-12T23:59:59.000Z

399

Physics 3, 84 (2010) A quantitative look into microorganism hydrodynamics  

E-Print Network [OSTI]

), are present in ev- ery part of the biosphere: giant plankton blooms in the oceans, protozoa in our lakes, microphytes (tiny plants such as microscopic algae), and protists (a di- verse group of single cell organisms

Bou-Zeid, Elie

400

airborne microorganisms laboratory: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the greatest potential of the Gulf to 2000 ft), which effectively traps most of the air pollution emitted from the surface1 Air Chemistry in the Gulf of Mexico Oil Spill Area...

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


401

TECHNICAL REPORTS Microorganisms are an integral part of the biogeochemical  

E-Print Network [OSTI]

proven to be effective in wastewater treatment, particularly for total suspended solids, organic matter to the small number of species that may be cultured. This CW was designed to treat rural eutrophic water processes have been used to treat the wastewater from large and small cities to protect the water

Zhang, Minghua

402

Material to Efficiently and Economically Obtain Microorganism and  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: VegetationEquipment Surfaces andMapping the Nanoscale LandscapeImports 5.90 4.86 4.77ofMaterial

403

Cofermentation with Cooperative Microorganisms for More Efficient Biomass  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation Proposed New SubstationClean Communities ofCellulosic Feedstock - EnergyCoal2

404

N e w E n g l a n d W a t e r T r e a t m e n t Technology Assistance Center  

E-Print Network [OSTI]

extraction wells in New Hampshire. 2. Characterize the treatment effectiveness of the slow sand filter.8-1.97 log Aerobic spore Forming Bacteria (CFU/100mL) 48 >2.4 log 8 2.3 log NA Turbidity (NTU) 48 82.8% 8 82 filtration extraction well water, even after eliminating the dilution effects with groundwater. The male

405

Tomitella biformata gen. nov., sp. nov., a new member of the suborder Corynebacterineae  

E-Print Network [OSTI]

, Hokkaido University, N9 W9, Kita-ku, Hokkaido 060-8589, Japan 2 Cold Regions Research and Engineering-reaction-positive, aerobic, non-spore-forming, irregular rod-shaped bacteria, designated AHU1821T and AHU1820, were isolated Corynebacterineae. The cell wall of strains AHU1821T and AHU1820 contained meso-diaminopimelic acid, arabinose

Douglas, Thomas A.

406

Glaciibacter superstes gen. nov., sp. nov., a novel member of the family Microbacteriaceae isolated  

E-Print Network [OSTI]

, Hokkaido University, N9 W9, Kita-ku, Hokkaido 060-8589, Japan 2 Cold Regions Research and Engineering-reaction-positive, aerobic, non-spore-forming, irregular rod-shaped bacteria, designated AHU1821T and AHU1820, were isolated Corynebacterineae. The cell wall of strains AHU1821T and AHU1820 contained meso-diaminopimelic acid, arabinose

Douglas, Thomas A.

407

Harvesting Energy from Wastewater in a 2-Chamber  

E-Print Network [OSTI]

Harvesting Energy from Wastewater in a 2-Chamber Microbial Fuel Cell Sikandar Present day wastewater treatment plants utilize high amounts of energy and are costly to operate. These conventional wastewater treatment plants utilize aerobic bacteria. Organic material in wastewater contains energy that can

408

Bacteria-targeting nanoparticles for managing infections  

E-Print Network [OSTI]

Bacterial infections continue to be a significant concern particularly in healthcare settings and in the developing world. Current challenges include the increasing spread of drug resistant (DR) organisms, the side effects ...

Radovic-Moreno, Aleksandar Filip

2013-01-01T23:59:59.000Z

409

Toxin-eating bacteria and bioremediation  

SciTech Connect (OSTI)

Methods are provided for reducing a level of one or more antibiotics from an antibiotic-contaminated substance. An organism that can utilize the one or more antibiotics as a carbon source is cultured in the presence of the antibiotic-contaminated substance for a sufficient amount of time to reduce the level of one or more antibiotics from the antibiotic-contaminated substance.

Church, George M; Dantas, Gautam; Sommer, Morten O

2014-02-25T23:59:59.000Z

410

Cloning systems for Rhodococcus and related bacteria  

DOE Patents [OSTI]

A plasmid transformation system for Rhodococcus was developed using an Escherichia coli-Rhodococcus shuttle plasmid. Rhodococcus sp. H13-A contains three cryptic indigenous plasmids, designated pMVS100, pMVS200 and pMVS300, of 75, 19.5 and 13.4 kilobases (Kb), respectively. A 3.8 Kb restriction fragment of pMVS300 was cloned into pIJ30, a 6.3 Kb pBR322 derivative, containing the E. coli origin of replication (ori) and ampicillin resistance determinant (bla) as well as a Streptomyces gene for thiostrepton resistance, tsr. The resulting 10.1 Kb recombinant plasmid, designated pMVS301, was isolated from E. coli DH1 (pMVS301) and transformed into Rhodococcus sp. AS-50, a derivative of strain H13-A, by polyethylene glycol-assisted transformation of Rhodococcus protoplasts and selection for thiostrepton-resistant transformants. This strain was deposited with the ATCC on Feb. 1, 1988 and assigned ATCC 53719. The plasmid contains the Rhodococcus origin of replication. The plasmid and derivatives thereof can therefore be used to introduce nucleic acid sequences to and from Rhodococcus for subsequent expression and translation into protein. The isolated origin of replication can also be used in the construction of new vectors.

Finnerty, William R. (Athens, GA); Singer, Mary E. (Decatur, GA)

1990-01-01T23:59:59.000Z

411

Cloning systems for Rhodococcus and related bacteria  

DOE Patents [OSTI]

A plasmid transformation system for Rhodococcus was developed using an Escherichia coli-Rhodococcus shuttle plasmid. Rhodococcus sp. H13-A contains three cryptic indigenous plasmids, designated pMVS100, pMVS200 and pMVS300, of 75, 19.5 and 13.4 kilobases (Kb), respectively. A 3.8 Kb restriction fragment of pMVS300 was cloned into pIJ30, a 6.3 Kb pBR322 derivative, containing the E. coli origin of replication (ori) and ampicillin resistance determinant (bla) as well as a Streptomyces gene for thiostrepton resistance, tsr. The resulting 10.1 Kb recombinant plasmid, designated pMVS301, was isolated from E. coli DH1 (pMVS301) and transformed into Rhodococcus sp. AS-50, a derivative of strain H13-A, by polyethylene glycol-assisted transformation of Rhodococcus protoplasts and selection for thiostrepton-resistant transformants. This strain was deposited with the ATCC on Feb. 1, 1988 and assigned ATCC 53719. The plasmid contains the Rhodococcus origin of replication. The plasmid and derivatives thereof can therefore be used to introduce nucleic acid sequences to and from Rhodococcus for subsequent expression and translation into protein. The isolated origin of replication can also be used in the construction of new vectors. 2 figs.

Finnerty, W.R.; Singer, M.E.

1990-08-28T23:59:59.000Z

412

Culturing photosynthetic bacteria through surface plasmon resonance  

SciTech Connect (OSTI)

In this work, cultivation of photosynthetic microbes in surface plasmon enhanced evanescent fields is demonstrated. Proliferation of Synechococcus elongatus was obtained on gold surfaces excited with surface plasmons. Excitation over three days resulted in 10 {mu}m thick biofilms with maximum cell volume density of 20% vol/vol (2% more total accumulation than control experiments with direct light). Collectively, these results indicate the ability to (1) excite surface-bound cells using plasmonic light fields, and (2) subsequently grow thick biofilms by coupling light from the surface. Plasmonic light delivery presents opportunities for high-density optofluidic photobioreactors for microalgal analysis and solar fuel production.

Ooms, Matthew D.; Bajin, Lauren; Sinton, David [Department of Mechanical and Industrial Engineering and Centre for Sustainable Energy, University of Toronto, Toronto M5S 3G8 (Canada)

2012-12-17T23:59:59.000Z

413

(Carbon monoxide metabolism by photosynthetic bacteria)  

SciTech Connect (OSTI)

Research continued on the metabolism of carbon monoxide by Rhodospirillum rubrum. This report discusses progress on the activity, induction, inhibition, and spectroscopic analysis of the enzyme Carbon Monoxide Dehydrogenase. (CBS)

Not Available

1989-01-01T23:59:59.000Z

414

Filling an emulsion drop with motile bacteria  

E-Print Network [OSTI]

We have measured the spatial distribution of motile Escherichia coli inside spherical water droplets emulsified in oil. At low cell concentrations, the cell density peaks at the water-oil interface; at increasing concentration, the bulk of each droplet fills up uniformly while the surface peak remains. Simulations and theory show that the bulk density results from a `traffic' of cells leaving the surface layer, increasingly due to cell-cell scattering as the surface coverage rises above $\\sim 10\\%$. Our findings show similarities with the physics of a rarefied gas in a spherical cavity with attractive walls.

I. D. Vladescu; E. J. Marsden; J. Schwarz-Linek; V. A. Martinez; J. Arlt; A. N. Morozov; D. Marenduzzo; M. E. Cates; W. C. K. Poon

2014-07-25T23:59:59.000Z

415

Sampling for Bacteria in Wells (Spanish)  

E-Print Network [OSTI]

C?mo Tomar Muestras de Pozos (para an?lisis bacteriol?gico) E-126S 11/01 Las muestras de agua para an?lisis bacteriol?gico siempre deben ser tomadas en envases esterilizados. El procedimiento para tomar las muestras de agua es el siguiente: 1...

Lesikar, Bruce J.

2001-11-15T23:59:59.000Z

416

How Bacteria Make Magnets | The Ames Laboratory  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation ProposedUsingFun withconfinement plasmas inPortalAllBPA addresses EMF B O N N E V I L L

417

Engineering Biofuels from Photosynthetic Bacteria - Energy Innovation  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation ProposedUsing Zirconia NanoparticlesSmart GrocerDepartment ofEngineer

418

Engineering Biofuels from Photosynthetic Bacteria | Argonne National  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation ProposedUsing Zirconia NanoparticlesSmart GrocerDepartment ofEngineerLaboratory

419

Sandia National Laboratories: measure bacteria attachment  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear Security Administration the1developmentturbine bladelifetime is the cumulative timemaximize energy3-D

420

Molecular probe technology detects bacteria without culture  

E-Print Network [OSTI]

EA, Miranda M, Aparicio AM, Fukushima M, Davis RW: MultiplexCN, Jiang H, Palm C, Fukushima M, Bernstein D, Vo KC,Page 10 of 10 12. Hyman RW, Fukushima M, Diamond L, Kumm J,

2012-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


421

On Ants, Bacteria and Dynamic Environments  

E-Print Network [OSTI]

while darker ones represent deep ..... deep valley and one peak, while F0b his the opposite. .... on the web address of a recent book from the same author.

Vitorino Ramos

2005-09-06T23:59:59.000Z

422

Final Project Report - Coupled Biogeochemical Process Evaluation for Conceptualizing Trichloriethylene Co-Metabolism: Co-Metabolic Enzyme Activity Probes and Modeling Co-Metabolism and Attenuation  

SciTech Connect (OSTI)

Trichloroethene (TCE) (also known as trichloroethylene) is a common contaminant in groundwater. TCE is regulated in drinking water at a concentration of 5 µg/L, and a small mass of TCE has the potential to contaminant large volumes of water. The physical and chemical characteristics of TCE allow it to migrate quickly in most subsurface environments, and thus large plumes of contaminated groundwater can form from a single release. The migration and persistence of TCE in groundwater can be limited by biodegradation. TCE can be biodegraded via different processes under either anaerobic or aerobic conditions. Anaerobic biodegradation is widely recognized, but aerobic degradation is less well recognized. Under aerobic conditions, TCE can be oxidized to non hazardous conditions via cometabolic pathways. This study applied enzyme activity probes to demonstrate that cometabolic degradation of TCE occurs in aerobic groundwater at several locations, used laboratory microcosm studies to determine aerobic degradation rates, and extrapolated lab-measured rates to in situ rates based on concentrations of microorganisms with active enzymes involved in cometabolic TCE degradation. Microcosms were constructed using basalt chips that were inoculated with microorganisms to groundwater at the Idaho National Laboratory Test Area North TCE plume by filling a set of Flow-Through In Situ Reactors (FTISRs) with chips and placing the FTISRs into the open interval of a well for several months. A parametric study was performed to evaluate predicted degradation rates and concentration trends using a competitive inhibition kinetic model, which accounts for competition for enzyme active sites by both a growth substrate and a cometabolic substrate. The competitive inhibition kinetic expression was programmed for use in the RT3D reactive transport package. Simulations of TCE plume evolution using both competitive inhibition kinetics and first order decay were performed.

Starr, Robert C; Orr, Brennon R; Lee, M Hope; Delwiche, Mark

2010-02-26T23:59:59.000Z

423

This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research  

E-Print Network [OSTI]

and endophyte were collected. The extract was inactive to the human parasites Trypanosoma cruzi, Leishmania reserved. Introduction Endophytes are microorganisms including bacteria or fungi that live within,2 Endophytic microbes have been recognized as important sources of structurally novel and biologically active

Coley, Phyllis

424

Volatile organic acids and microbial processes in the Yegua formation, east-central Texas  

E-Print Network [OSTI]

and Geophysics, Texas A&M University, College Station, TX, 77843, USA b Institute for Energy and the EnvironmentVolatile organic acids and microbial processes in the Yegua formation, east-central Texas Joyanto production of VOAs by microorganisms in mudstones, and net consumption of VOAs by SO4 reducing bacteria (SRB

Grossman, Ethan L.

425

Application of cyclic voltammetry to investigate enhanced catalytic current generation by biofilm-modified anodes of Geobacter sulfurreducens strain  

E-Print Network [OSTI]

Microbial fuel cells (MFCs) exploit the ability of some bacteria to couple oxidation of organic matter Microbial fuels cells (MFCs) rely on the ability of certain microorganisms to transfer electrons to anodes is broadly applicable and may be useful to develop strategies for optimizing power generation by MFCs. 896

Lovley, Derek

426

257Agron. Sustain. Dev. 26 (2006) 257262 INRA, EDP Sciences, 2006  

E-Print Network [OSTI]

JAVAID* Department of Mycology and Plant Pathology, University of the Punjab, Quaid-e-Azam Campus Lahore. INTRODUCTION Fertilizers as a source of plant nutrients and pesticides as plant protection measures are being of co-existing beneficial microorganisms, the main ones being the species of photosynthetic bacteria

Boyer, Edmond

427

Refreshments will be served For more information contact Kim Coleman at kcole@wustl.edu  

E-Print Network [OSTI]

of thousands of sites in the US alone. About 15 years ago, bacteria that were able to "breathe" some substances in sediment, groundwater, and wastewater. She is particularly interested in which organisms microorganisms to treat wastewater. Dr. Novak works both in the laboratory and in the field. determining whether

Subramanian, Venkat

428

Bulletin of Mathematical Biology (2010) DOI 10.1007/s11538-010-9536-1  

E-Print Network [OSTI]

in global warming. The metabolism of microorganisms found in the oceans is a significant Corresponding. This behavior induces a fluid flow that, in turn, causes the bacteria to aggregate leading to the formation integral method 1. Introduction Understanding the underlying processes which influence our global climate

Wolgemuth, Charles

429

Fax +41 61 306 12 34 E-Mail karger@karger.ch  

E-Print Network [OSTI]

- lulosic biomass [Schneider, 1989]. There are many re- ports of microorganisms such as bacteria and fungi treated for 21 days with these 5 strains showed a preference for consumption of hemicelluloses over lignin and me- chanical wounds. Lignocellulosic biomasses such as agricultural resi- dues and energy crops

Qin, Wensheng

430

Original article Utilization of algal polysaccharides by human  

E-Print Network [OSTI]

microorganisms, the large increase in acetate produc- tion observed in the coculture with acetogenic bacteria was mainly due to acetogenic fermentation of sugars released during laminaran hydrolysis. human colon'acétate observée dans la coculture avec la bactérie acétogène s'explique principalement par la fermentation

Paris-Sud XI, Université de

431

Heterotrophic microbial activity in lake sediments: effects of organic electron donors  

E-Print Network [OSTI]

by competition for fermentation products such as H2, CO2 and acetate. Microorganisms derive energy production and further break down monomers to alcohols, fatty acids, and hydrogen (H2) through fermentation. Alcohols and fatty acids are then further degraded by syntrophic bacteria (secondary ferment- ers

Florida, University of

432

Temperature-driven decoupling of key phases of organic matter degradation in marine sediments  

E-Print Network [OSTI]

organic matter is further hydrolyzed and fermented to LMW-DOC, such as volatile fatty acids (VFAs) (2 (9­12). The organic carbon consumed by sulfate- reducing bacteria is often VFAs, such as acetic acid is recycled or buried. A diverse consor- tium of microorganisms that hydrolyze, ferment, and terminally

Weston, Nathaniel B.

433

Biogenic gas nanostructures as ultrasonic molecular reporters  

E-Print Network [OSTI]

Biogenic gas nanostructures as ultrasonic molecular reporters Mikhail G. Shapiro1,2,3 *, Patrick W on the nanoscale. Here, we introduce a new class of reporters for ultrasound based on genetically encoded gas nanostructures from microorganisms, including bacteria and archaea. Gas vesicles are gas-filled protein

Schaffer, David V.

434

Removal of mercury from coal via a microbial pretreatment process  

SciTech Connect (OSTI)

A process for the removal of mercury from coal prior to combustion is disclosed. The process is based on use of microorganisms to oxidize iron, sulfur and other species binding mercury within the coal, followed by volatilization of mercury by the microorganisms. The microorganisms are from a class of iron and/or sulfur oxidizing bacteria. The process involves contacting coal with the bacteria in a batch or continuous manner. The mercury is first solubilized from the coal, followed by microbial reduction to elemental mercury, which is stripped off by sparging gas and captured by a mercury recovery unit, giving mercury-free coal. The mercury can be recovered in pure form from the sorbents via additional processing.

Borole, Abhijeet P. (Knoxville, TN); Hamilton, Choo Y. (Knoxville, TN)

2011-08-16T23:59:59.000Z

435

E-Print Network 3.0 - aerobic microbial cometabolism Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Solvents... .D., Environmental Engineering and Science 1991 Stanford University, Palo Alto, CA Thesis Research: Cometabolic... biological processes, and environmental...

436

E-Print Network 3.0 - aerobic exercise deconditioning Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Collection: Engineering ; Geosciences 19 Page Mill YMCA 755 Page Mill Rd. Bldg B, Palo Alto Summary: permission for exercise specialist to call physician or person giving...

437

A KINETIC STUDY OF AEROBIC PROPANE UPTAKE AND COMETABOLIC DEGRADATION OF CHLOROFORM,  

E-Print Network [OSTI]

and LEWIS SEMPRINI2 1Department of Chemical and Mining Engineering and Environmental Technologies., 1994; Wilcox et al., 1995; Wackett et al., 1989). These studies report longer lag Water, Air, and Soil Pollution: Focus 3: 285­298, 2003. © 2003 Kluwer Academic Publishers. Printed in the Netherlands. #12;286 D

Semprini, Lewis

438

Biogeochemical modelling of anaerobic vs. aerobic methane oxidation in a meromictic crater lake (Lake Pavin, France)  

E-Print Network [OSTI]

Géosciences, 1A rue de la Férolerie, 45071 Orléans Cedex 2, France Abstract Methane is a powerful greenhouse gas and its concentration in the atmosphere has increased over the past decades. Methane produced

Boyer, Edmond

439

E-Print Network 3.0 - aerobic nonsulfur photosynthetic Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

& Universit Pierre-et-Marie-Curie, Paris 6 Collection: Biology and Medicine ; Renewable Energy 5 Research Focus Coloring in the tree of life Summary: characterized bacterial...

440

E-Print Network 3.0 - aerobic photosynthetic bacterium Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

& Universit Pierre-et-Marie-Curie, Paris 6 Collection: Biology and Medicine ; Renewable Energy 3 Research Focus Coloring in the tree of life Summary: -green photosynthetic...

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

E-Print Network 3.0 - aerobic anoxygenic photosynthetic Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

& Universit Pierre-et-Marie-Curie, Paris 6 Collection: Biology and Medicine ; Renewable Energy 9 Research Focus Coloring in the tree of life Summary: characterized bacterial...

442

E-Print Network 3.0 - aerobic common ancestor Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Environmental Sciences and Ecology 8 An Assessment of the Impacts of Molecular Oxygen on the Evolution of Sara Vieira-Silva* and Eduardo P. C. Rocha* Summary: the...

443

E-Print Network 3.0 - acute aerobic exercise Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the heart rate) What are some examples of ... Source: Wilkins, Neal - Institute of Renewable Natural Resources & Department of Wildlife and Fisheries Sciences, Texas A&M...

444

E-Print Network 3.0 - aerobic exercise resistance Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the heart rate) What are some examples of ... Source: Wilkins, Neal - Institute of Renewable Natural Resources & Department of Wildlife and Fisheries Sciences, Texas A&M...

445

E-Print Network 3.0 - aerobic exercise training Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

California State University, Fullerton Collection: Chemistry 43 Evidence ReportTechnology Assessment Effectiveness of Behavioral Interventions to Modify Summary: , with...

446

E-Print Network 3.0 - aerobic exercise program Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

that like the biceps, the heart is a muscle, and ... Source: Wilkins, Neal - Institute of Renewable Natural Resources & Department of Wildlife and Fisheries Sciences, Texas A&M...

447

E-Print Network 3.0 - aerobic physical exercise Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

or drawing paper, markers, tape Activity: Remind ... Source: Wilkins, Neal - Institute of Renewable Natural Resources & Department of Wildlife and Fisheries Sciences, Texas A&M...

448

E-Print Network 3.0 - aerobic exercise parameters Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the heart rate) What are some examples of ... Source: Wilkins, Neal - Institute of Renewable Natural Resources & Department of Wildlife and Fisheries Sciences, Texas A&M...

449

E-Print Network 3.0 - aerobic exercise combined Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the heart rate) What are some examples of ... Source: Wilkins, Neal - Institute of Renewable Natural Resources & Department of Wildlife and Fisheries Sciences, Texas A&M...

450

E-Print Network 3.0 - aerobic exercising program Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

that like the biceps, the heart is a muscle, and ... Source: Wilkins, Neal - Institute of Renewable Natural Resources & Department of Wildlife and Fisheries Sciences, Texas A&M...

451

E-Print Network 3.0 - aerobic training resistance Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Columbia University - Waste-to-Energy Research and Technology Council (WTERT) Collection: Renewable Energy 28 p y ich s ia tat vc il yp y ich s ia tat vc il y The university...

452

E-Print Network 3.0 - aerobic exercise acutely Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the heart rate) What are some examples of ... Source: Wilkins, Neal - Institute of Renewable Natural Resources & Department of Wildlife and Fisheries Sciences, Texas A&M...

453

E-Print Network 3.0 - aerobically growing pseudomonas Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Yale University Collection: Environmental Management and Restoration Technologies 17 Water Research 38 (2004) 673680 Chlorate and nitrate reduction pathways are separately...

454

E-Print Network 3.0 - aerobic microcosms collected Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Microbial response was evaluated as a function of microcosm mineralogy, geochemical environment... inclusions were added to the microcosm, while consumption of carbon...

455

E-Print Network 3.0 - aerobic conditions Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

that received biosolids (anaerobic and aerobiclime stabilized... soils containing sewage sludge under laboratory conditions (Topp and Starratt, 2000), but within 2-cm......

456

E-Print Network 3.0 - aerobic microbial enhanced Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Persistence and Migration of Alkylphenol Ethoxylate Degradation Products Associated with Land-applied Biosolids Summary: during runoff events from pasturelands that received...

457

E-Print Network 3.0 - aerobically-poised hepg2 cells Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

a distinct manner Summary: (ERh) transfected into the human HepG2 hepatoma cell line and expanded the study in vivo to examine... -estrogen; Receptor; Bisphenol A; HepG2 cells;...

458

E-Print Network 3.0 - aerobic treatment units Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Summary: 1355770X0600341X Printed in the United Kingdom Optimization model for integrated municipal solid waste... for the United Kingdom refused derived fuel was found to be the...

459

E-Print Network 3.0 - aerobic biological treatment Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Environmental Engineering, University of Hawai'i at Manoa Collection: Engineering ; Energy Storage, Conversion and Utilization 51 Phenotypic flexibility in passerine birds:...

460

E-Print Network 3.0 - aerobic training program Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

of water per year. A new training program on chemigation... participants. The rainwater harvesting program continued to grow and develop. Trainings included...

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


461

E-Print Network 3.0 - aerobic interval training Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

on the proper operation and maintenance of on-site wastewater treatment systems... , rainwater harvesting, and bacterial sources in water. Provided a homeowner training...

462

Redox and ATP control of photosynthetic cyclic electron flow in Chlamydomonas reinhardtii (I) aerobic conditions  

E-Print Network [OSTI]

Redox and ATP control of photosynthetic cyclic electron flow in Chlamydomonas reinhardtii (I, requires the production of ATP and NADPH in a ratio of 3:2. The oxygenic photosynthetic chain can function following two different modes: the linear electron flow which produces reducing power and ATP

463

Aerobic Exercise Training Reduces Cannabis Craving and Use in Non-Treatment Seeking Cannabis-Dependent  

E-Print Network [OSTI]

,4 , Ronald L. Cowan2,3,5,6 , Peter R. Martin2 1 Energy Balance Laboratory, Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, Vanderbilt University, Nashville, Tennessee, United States University, Nashville, Tennessee, United States of America, 3 Department of Psychology, Vanderbilt University

Palmeri, Thomas

464

aerobic vinyl chloride-assimilating: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

organic sulfides,6 and perfluorinated polyesters,7,8 have been used to create release coatings Chaudhury, Manoj K. 425 1283J. Blumel, F. H. Kohler Metallated...

465

E-Print Network 3.0 - aerobic spore formers Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

heat resistance of Clostridium sporogenes Jae-Hyung Mah a Summary: , the addition of CaCl2 showed a slightly, but not significantly, greater increase in spore levels within 3...

466

E-Print Network 3.0 - aerobic nonylphenol degradation Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

et al. 2006) has led to an intense scientific and public debate... . Emissions from UV irradiation are almost instantaneous, indicating a direct degradating photochemical......

467

E-Print Network 3.0 - aerobic vanillate degradation Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

et al. 2006) has led to an intense scientific and public debate... . Emissions from UV irradiation are almost instantaneous, indicating a direct degradating photochemical......

468

E-Print Network 3.0 - aerobic microbial degradation Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

et al. 2006) has led to an intense scientific and public debate... . Emissions from UV irradiation are almost instantaneous, indicating a direct degradating photochemical......

469

E-Print Network 3.0 - aerobic mitochondrial energy Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

exercise ... Source: Leary, Scot - Department of Biochemistry, University of Saskatchewan Collection: Biology and Medicine 4 Mitochondrial biogenesis: which part of NO''...

470

A comparison of the histamine production in fish packaged aerobically and anaerobically  

E-Print Network [OSTI]

seafood caught in favorable areas is shipped, either processed or unprocessed, to distant markets. Experience has shown that food-poisoning problems may arise when preservation procedures are instituted in preparing and storing foods. Thus when new... products are introduced, potential food- poisoning must be investigated. The citations on the following pages follow the style of the Journal of Food Science. Recent incidences of histamine poisoning in canned tuna have in- creased the awareness...

Lu, Shin

2012-06-07T23:59:59.000Z

471

E-Print Network 3.0 - aerobic microbial metabolism Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

or an added artificial mediator that facilitates electron transfer... in the microbial fuel cells at the time, in which the reduced end products of microbial metabolism or...

472

E-Print Network 3.0 - aerobic co-oxidizing thermophile Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Life LUCA may be a Thermophile Possibly more complex than some existing life 12;The Tree of Life 12... ) Alkalophiles - inhabit alkaline lakes (pH10) Barophiles -...

473

E-Print Network 3.0 - autoheated thermophilic aerobic Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Life LUCA may be a Thermophile Possibly more complex than some existing life 12;The Tree of Life 12... ) Alkalophiles - inhabit alkaline lakes (pH10) Barophiles -...

474

E-Print Network 3.0 - aerobic granular sewage Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

from... -lot sewage systems concern the soil. For the soil to properly renovate the treatment tank's effluent, two Source: Boyer, Elizabeth W. - School of Forest Resources,...

475

E-Print Network 3.0 - aerobic mtbe biodegradation Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

27 ACCEPTED BY WATER ENVIRONMENT RESEARCH ODOR AND VOC REMOVAL FROM WASTEWATER TREATMENT PLANT Summary: capable of MTBE biodegradation. 12;Effective treatment of a mixture...

476

E-Print Network 3.0 - aerobic granular sbr Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Biodegradation of 4-nitrophenol in a two-phase sequencing Summary: operating in wastewater treatment plants. Keywords TPPBSBR bioreactor. Xenobiotic biodegradation . 4... ....

477

E-Print Network 3.0 - aerobic membrane bioreactor Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Bioreactor: a Multi... . Childress, "The Novel Osmotic Membrane Bioreactor for Wastewater Treatment", WEFTEC Conference, October 18... Osmotic Membrane Bioreactor: a Dual...

478

E-Print Network 3.0 - aerobic activated sludge Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Effects on Waste Activated Sludge Digestion Thomas... activated sludge (WAS) from wastewater treatment plants (WWTP) demands extensive sludge dewatering... structure and are...

479

E-Print Network 3.0 - aerobic granular sludge Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

71(11):7523-7527. Delong EF (1992) Archaea in coastal marine... in a Municipal Wastewater Sludge Daniel Williams1 and James W. Brown2* 1 Department of Biology, North...

480

E-Print Network 3.0 - aerobic nitrogen cycle Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

biological nitrogen fixation of gaseous nitrogen. The water cycle is important to ecosystem... and Nitrogen Cycles As ... Source: Barboza, Perry - Institute of Arctic Biology,...

Note: This page contains sample records for the topic "aerobic bacteria microorganisms" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


481

Serum phospholipid response to aerobic exercise training in pre- and postmenopausal women  

E-Print Network [OSTI]

. . . . . . . . . . . . . . . . . 48 50 56 62 81 . 83 LIST OF TABLES Page TABLE 1. Physical Characteristics of Subjects in the Exercise Group. . . . . . . . . . . . . . . 32 TABLE 2. Statistical Analysis of Subjects' Physical Charactenstics. . . . TABLE 3. Cholesterol... Phospholipids Based on Menopausal Status. . . . 42 TABLE 9. Statistical Companson of P-Values of the Sub-Groups. . . . . . . . . . . 43 CHAPTER I INTRODUCTION Cardiovascular diseases (CVD) remain the number one cause of death for both men and women...

Kamath, Adrian Elise

1995-01-01T23:59:59.000Z

482

E-Print Network 3.0 - aerobic microbial ecosystems Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Starch Glucose Complex anaerobic... 12;Microbial Systems as Model Ecosystems P C R Energy Heat ... Source: Vallino, Joseph J. - Ecosystems Center, Marine Biological...

483

E-Print Network 3.0 - aerobic actinomycetes epidemiology Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Evolution, Collection: Biology and Medicine ; Environmental Sciences and Ecology 88 Bioenergy Initiative Directory 2008 Areas of Research Expertise Summary: - Zoology Prof Ian...

484

E-Print Network 3.0 - aerobic bacteriochlorophyll a-producing...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

; Environmental Sciences and Ecology 36 pubs.acs.orgBiochemistry Published on Web 08212009 r 2009 American Chemical Society 9132 Biochemistry 2009, 48, 91329139...

485

E-Print Network 3.0 - aerobic nitrogen fixation Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Collection: Mathematics 85 Nitrogen isotope dynamics of the Cariaco Basin, Venezuela Robert C. Thunell,1 Summary: . In recent budgets of oceanic fixed nitrogen, N2...

486

E-Print Network 3.0 - aerobic shallow aquifer Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Geological Survey, National Research Program Collection: Multidisciplinary Databases and Resources ; Environmental Sciences and Ecology ; Geosciences 33 2, 917938, 2005 Nonlinear...

487

E-Print Network 3.0 - aerobic endurance training Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

sport throughout the competitive season is difficult; more so when your squad only trains on a part Source: Howie, Jim - Department of Mathematics, Heriot-Watt University...

488

E-Print Network 3.0 - aerobic wwtps sited Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

plants (WWTPs). The amounts of methane or methane potential that are recovered by biogas... is an estimate of the actual amount of emitted methane during wastewater treatment...

489

E-Print Network 3.0 - aerobic digestion Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Council (WTERT) Collection: Renewable Energy 8 www.manuremanagement.cornell.edu Biogas Casebook Summary: Riverhead, NY 11901 Corwin Duck FarmCorwin Duck Farm Digester type...

490

E-Print Network 3.0 - autothermal aerobic digestion Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Council (WTERT) Collection: Renewable Energy 12 www.manuremanagement.cornell.edu Biogas Casebook Summary: Riverhead, NY 11901 Corwin Duck FarmCorwin Duck Farm Digester type...

491

E-Print Network 3.0 - aerobic hydrogen accumulation Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

accumulation in the system (9). If high concentrations... to be measured as total biogas pro- duction. Hydrogen gas does not ordinarily ... Source: Logan, Bruce E.- Department...

492

E-Print Network 3.0 - aerobic digestion atad Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Council (WTERT) Collection: Renewable Energy 11 www.manuremanagement.cornell.edu Biogas Casebook Summary: Riverhead, NY 11901 Corwin Duck FarmCorwin Duck Farm Digester type...

493

E-Print Network 3.0 - aerobic digestion process Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Technologies ; Environmental Sciences and Ecology 9 www.manuremanagement.cornell.edu Biogas Casebook Summary: of gas handling equipment for an anaerobic digester system....

494

E-Print Network 3.0 - aerobic gram-negative bacilli Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the trophic interaction between free-living protozoa and Gram-negative bacte- ria... in the training range soil ...8 Figure 6. PLFA biomarkers for Gram-negative...

495

aerobic packed-bed biofilm: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Formation Dietrich, Lars 98 Application of a moving bed biofilm reactor for tertiary ammonia treatment in high temperature industrial wastewater Environmental Management and...

496

E-Print Network 3.0 - aerobic batch fermentations Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Program Collection: Energy Storage, Conversion and Utilization ; Renewable Energy 55 Troubleshooting Silage Problems: How to Identify Potential Problems Summary: Active...

497

Biological enhancement of hydrocarbon extraction  

DOE Patents [OSTI]

A method of microbial enhanced oil recovery for recovering oil from an oil-bearing rock formation is provided. The methodology uses a consortium of bacteria including a mixture of surfactant producing bacteria and non-surfactant enzyme producing bacteria which may release hydrocarbons from bitumen containing sands. The described bioprocess can work with existing petroleum recovery protocols. The consortium microorganisms are also useful for treatment of above oil sands, ground waste tailings, subsurface oil recovery, and similar materials to enhance remediation and/or recovery of additional hydrocarbons from the materials.

Brigmon, Robin L. (North Augusta, SC); Berry, Christopher J. (Aiken, SC)

2009-01-06T23:59:59.000Z

498

A model for gyrotactic pattern formation of motile micro-organisms in turbulence  

E-Print Network [OSTI]

Recent studies show that the dynamics of motile organisms subject to gravitational torques in turbulence gives rise to patchiness. Spherical motile organisms gather in down-welling regions of the turbulent flow. We determine how shape affects preferential sampling and small-scale spatial clustering (determining local encounter rates) by analysing a statistical model in two and three spatial dimensions. By recursively refining approximations for the paths the organisms take through the flow we determine analytically how preferential sampling and small-scale clustering in the model depend upon the dimensionless parameters of the problem. We show that singularities ("caustics") occur in the dynamics and discuss how these singularities affect spatial patterns.

Gustavsson, K; Jonsson, P R; Mehlig, B

2015-01-01T23:59:59.000Z

499

NUMERICAL MODELLING OF MICROORGANISMS DISPERSION IN URBAN AREA: APPLICATION TO LEGIONELLA.  

E-Print Network [OSTI]

with transmission of an infectious agent from cooling towers (CT). During the episode that occured in Pas to contaminated cooling towers system may occur over distance larger than 10km. In addition, most cooling towers dispersion from a virtual cooling tower at the same location. The biological model has been activated

Paris-Sud XI, Université de

500

Development of systematic and combinatorial approaches for the metabolic engineering of microorganisms  

E-Print Network [OSTI]

Explorations and optimizations through the genomic space are a daunting undertaking given the complexity and size of the possible search space. To approach this problem, systematic and combinatorial approaches were employed ...

Alper, Hal (Hal Samuel)

2006-01-01T23:59:59.000Z