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Sample records for ad alzheimer disease

  1. New Clues in Predicting Alzheimer's Disease

    ScienceCinema (OSTI)

    Jagust, William

    2013-05-29

    Theres a new clue in the search to identify the harbingers of Alzheimers disease. More info: http://newscenter.lbl.gov/feature-stories/2008/12/16/predict-alzheimers-disease/

  2. Reductions in (/sup 3/H)nicotinic acetylcholine binding in Alzheimer's disease and Parkinson's disease: an autoradiographic study

    SciTech Connect (OSTI)

    Whitehouse, P.J.; Martino, A.M.; Wagster, M.V.; Price, D.L.; Mayeux, R.; Atack, J.R.; Kellar, K.J.

    1988-05-01

    In Alzheimer's disease (AD) and Parkinson's disease (PD), dysfunction in the basal forebrain cholinergic system is accompanied by a consistent loss of presynaptic cholinergic markers in cortex, but changes in cholinergic receptor binding sites are poorly understood. In the present study, we used receptor autoradiography to map the distribution of nicotinic (/sup 3/H)acetylcholine binding sites in cortices of individuals with AD and PD and matched control subjects. In both diseases, a profound loss of nicotinic receptors occurs in all cortical layers, particularly the deepest layers.

  3. Nanoimaging to Prevent and Treat Alzheimers and Parkinsons Diseases. Scientific/Technical report

    SciTech Connect (OSTI)

    Yuri L. Lyubchenko, PhD, DSc

    2012-12-20

    This project will develop innovative approaches to characterization of the very early stages of protein aggregation that eventually can be translated to the development of early diagnostic tools and efficient treatments for Alzheimers, Parkinsons and Huntingtons diseases. Funding will be used to acquire nanoimaging technology for nanoscale imaging, manipulation and analysis of biomedical materials to develop treatments that will repair disabled proteins and cure diseases that result from protein malfunction, specifically Alzheimers and Parkinsons diseases. Expected outcomes include tests for early diagnosis and therapeutic treatments for these devastating neurological diseases. To elucidate the mechanisms of protein misfolding, we will establish an extensive program of experimental studies using a broad arsenal of advanced nanoscale and traditional techniques that will be integrated with molecular-scale modeling of protein misfolding and the nucleation of aggregate structures. To identify intracellular machinery or/and multicomponent complexes critically involved in protein misfolding, we will characterize interactions between targeted proteins and specific intracellular components or metabolites that impact on protein conformational pathways leading to protein misfolding accompanied by formation of toxic aggregated morphologies. To design innovative nanotechnology tools for the control of intracellular protein misfolding and aggregation processes, we will develop a predictive molecular scale model for intracellular protein misfolding and the formation of toxic aggregates. Verified through experimental studies, the objective is to establish an enabling foundation for the engineering of novel molecular diagnostics and therapeutics for various cellular pathologies.

  4. DEVELOPMENT OF AUTOMATED SOFTWARE PROGRAM FOR THE ANALYSIS OF ALZHEIMER'S DISEASE BETA-AMYLOID SCANS

    SciTech Connect (OSTI)

    Mariotti, Jack; Zubal, George

    2013-12-18

    Study goal: A Phase 1 evaluation of the kinetics, clearance and cerebral distribution of one novel peripheral benzodiazepine receptors(PBR)positron emission tomography (PET) imaging agent, 18F-PBR-111 following intravenous administration in healthy volunteers and Alzheimer's disease (AD) patients. Short title: Evaluation of PET imaging with PBR-111 in HV and AD subjects Proof of Mechanism. Primary Objective: To evaluate the cerebral distribution of PBR-111 positron emission tomography (PET) for detection/exclusion of microglial activation in patients with Alzheimer's disease subjects compared to healthy volunteers. Secondary objectives: - To assess the dynamic uptake and washout of [18F]PBR-111, a potential imaging bio-marker for inflammatory changes in brain, using positron emission tomography in subjects with Alzheimer's disease (AD) and healthy volunteers (HV). - To perform blood metabolite characterization of [18F]PBR-111 in subjects with AD and HV to determine the nature of metabolites in assessment of [18F]-PBR-111 as a PET brain imaging agent. Name of radioactive drug substance: PBR-111 Dose(s): The applied PBR-111 radioactive dose will be up to 5.0 mCi, diluted in a maximum of 10 ml of saline. The radioligand will be administered as a slow intravenous bolus injection (i.e., 6 sec/ml) into a large vein (e.g., antecubital vein). Route of administration: Intravenous injection Duration of treatment: Single administration of a diagnostic agent Indication: PBR-111 positron emission tomography (PET) imaging has the potential to detect microglial activation. In the presence of PBR-111 uptake (representative of microglial activation), inflammation in the brain can be detected. Diagnosis and main criteria for inclusion: Study participants will be HVs and patients diagnosed with probable AD. HVs must be 18 years of age (at least four subjects 50 years of age) and have no evidence of cognitive impairment or other neurologic disease by medical history. The lack of

  5. Metabonomic Profiling of TASTPM Transgenic Alzheimer's Disease Mouse Model

    SciTech Connect (OSTI)

    Hu, Zeping; Browne, Edward R.; Liu, Tao; Angel, Thomas E.; Ho, Paul C.; Chun Yong Chan, Eric

    2012-12-07

    Identification of molecular mechanisms underlying early stage Alzheimer’s disease (AD) is important for the development of new therapies against and diagnosis of AD. In this study, non-targeted metabotyping of TASTPM transgenic AD mice was performed. The metabolic profiles of both brain and plasma of TASTPM mice were characterized using gas chromatography-mass spectrometry and compared to those of wild type C57BL/6J mice. TASTPM mice were metabolically distinct compared to wild type mice (Q28 Y = 0.587 and 0.766 for PLS-DA models derived from brain and plasma, respectively). A number of metabolites were found to be perturbed in TASTPM mice in both brain (D11 fructose, L-valine, L-serine, L-threonine, zymosterol) and plasma (D-glucose, D12 galactose, linoleic acid, arachidonic acid, palmitic acid and D-gluconic acid). In addition, enzyme immunoassay confirmed that selected endogenous steroids were significantly perturbed in brain (androstenedione and 17-OH-progesterone) and plasma (cortisol and testosterone) of TASTPM mice. Ingenuity pathway analysis revealed that perturbations related to amino acid metabolism (brain), steroid biosynthesis (brain), linoleic acid metabolism (plasma) and energy metabolism (plasma) accounted for the differentiation of TASTPM and wild-type

  6. Fourier Transform Infrared Imaging Showing Reduced Unsaturated Lipid Content in the Hippocampus of a mouse Model of Alzheimer's Disease

    SciTech Connect (OSTI)

    Leskovjan, A.C.; Kretlow, A.; Miller, L.M.

    2010-04-01

    Polyunsaturated fatty acids are essential to brain functions such as membrane fluidity, signal transduction, and cell survival. It is also thought that low levels of unsaturated lipid in the brain may contribute to Alzheimer's disease (AD) risk or severity. However, it is not known how accumulation of unsaturated lipids is affected in different regions of the hippocampus, which is a central target of AD plaque pathology, during aging. In this study, we used Fourier transform infrared imaging (FTIRI) to visualize the unsaturated lipid content in specific regions of the hippocampus in the PSAPP mouse model of AD as a function of plaque formation. Specifically, the unsaturated lipid content was imaged using the olefinic {double_bond}CH stretching mode at 3012 cm{sup -1}. The axonal, dendritic, and somatic layers of the hippocampus were examined in the mice at 13, 24, 40, and 56 weeks old. Results showed that lipid unsaturation in the axonal layer was significantly increased with normal aging in control (CNT) mice (p < 0.01) but remained low and relatively constant in PSAPP mice. Thus, these findings indicate that unsaturated lipid content is reduced in hippocampal white matter during amyloid pathogenesis and that maintaining unsaturated lipid content early in the disease may be critical in avoiding progression of the disease.

  7. A State-Wide Research Network for Alzheimer's Disease

    SciTech Connect (OSTI)

    Mintzer, Jacobo E.; Bachman, D. L.; Stuckey, M.; Ebeling, M.; Wagner, M. T.; Evans, W. J.; Hirth, V.; Walker, A.; Joglekar, R.; Faison, W.

    2014-03-13

    The Specific Aim of the proposal was to develop an administrative structure that will facilitate the development of AD research across the state of SC by providing key services such as (but not limited to) seeking funding research opportunities, financial tracking, regulatory management, central recruitment, training for investigators and coordinators, data collection, data storing, and data processing to researchers across the state.

  8. Key residues for the oligomerization of A{beta}42 protein in Alzheimer's disease

    SciTech Connect (OSTI)

    Ngo, Sam; Guo, Zhefeng

    2011-10-28

    Highlights: Black-Right-Pointing-Pointer A{beta} oligomers are neurotoxins and likely the causing agents for Alzheimer's disease. Black-Right-Pointing-Pointer A{beta}42 fusion protein form globular oligomers. Black-Right-Pointing-Pointer A{beta}42 fusion protein oligomers contain SDS-resistant tetramers and hexamers. Black-Right-Pointing-Pointer Cysteine substitutions at residues 31, 32, 34, 39-41 disrupt A{beta}42 oligomerization. -- Abstract: Deposition of amyloid fibrils consisting of amyloid {beta} (A{beta}) protein as senile plaques in the brain is a pathological hallmark of Alzheimer's disease. However, a growing body of evidence shows that soluble A{beta} oligomers correlate better with dementia than fibrils, suggesting that A{beta} oligomers may be the primary toxic species. The structure and oligomerization mechanism of these A{beta} oligomers are crucial for developing effective therapeutics. Here we investigated the oligomerization of A{beta}42 in the context of a fusion protein containing GroES and ubiquitin fused to the N-terminus of A{beta} sequence. The presence of fusion protein partners, in combination with a denaturing buffer containing 8 M urea at pH 10, is unfavorable for A{beta}42 aggregation, thus allowing only the most stable structures to be observed. Transmission electron microscopy showed that A{beta}42 fusion protein formed globular oligomers, which bound weakly to thioflavin T and Congo red. SDS-PAGE shows that A{beta}42 fusion protein formed SDS-resistant hexamers and tetramers. In contrast, A{beta}40 fusion protein remained as monomers on SDS gel, suggesting that the oligomerization of A{beta}42 fusion protein is not due to the fusion protein partners. Cysteine scanning mutagenesis at 22 residue positions further revealed that single cysteine substitutions of the C-terminal hydrophobic residues (I31, I32, L34, V39, V40, and I41) led to disruption of hexamer and tetramer formation, suggesting that hydrophobic interactions between these

  9. OSTIblog Articles in the Alzheimer's Topic | OSTI, US Dept of Energy Office

    Office of Scientific and Technical Information (OSTI)

    of Scientific and Technical Information Alzheimer's Topic Sharing Data Leads to Progress by Nena Moss 19 Aug, 2010 in Personal Perspectives My mother died in March 2010 after a 15-year battle with Alzheimer's, so I pay particular attention to news about this dreadful disease. A recent New York Times article caught my eye: "Sharing of Data Leads to Progress on Alzheimer's." How did sharing data lead to progress on Alzheimer's? A collaborative effort, the Alzheimer's Disease

  10. Noggin and BMP4 co-modulate adult hippocampal neurogenesis in the APP{sub swe}/PS1{sub {Delta}E9} transgenic mouse model of Alzheimer's disease

    SciTech Connect (OSTI)

    Tang, Jun; Song, Min; Wang, Yanyan; Fan, Xiaotang; Xu, Haiwei; Bai, Yun

    2009-07-31

    In addition to the subventricular zone, the dentate gyrus of the hippocampus is one of the few brain regions in which neurogenesis continues into adulthood. Perturbation of neurogenesis can alter hippocampal function, and previous studies have shown that neurogenesis is dysregulated in Alzheimer disease (AD) brain. Bone morphogenetic protein-4 (BMP4) and its antagonist Noggin have been shown to play important roles both in embryonic development and in the adult nervous system, and may regulate hippocampal neurogenesis. Previous data indicated that increased expression of BMP4 mRNA within the dentate gyrus might contribute to decreased hippocampal cell proliferation in the APP{sub swe}/PS1{sub {Delta}E9} mouse AD model. However, it is not known whether the BMP antagonist Noggin contributes to the regulation of neurogenesis. We therefore studied the relative expression levels and localization of BMP4 and its antagonist Noggin in the dentate gyrus and whether these correlated with changes in neurogenesis in 6-12 mo old APP{sub swe}/PS1{sub {Delta}E9} transgenic mice. Bromodeoxyuridine (BrdU) was used to label proliferative cells. We report that decreased neurogenesis in the APP/PS1 transgenic mice was accompanied by increased expression of BMP4 and decreased expression of Noggin at both the mRNA and protein levels; statistical analysis showed that the number of proliferative cells at different ages correlated positively with Noggin expression and negatively with BMP4 expression. Intraventricular administration of a chimeric Noggin/Fc protein was used to block the action of endogenous BMP4; this resulted in a significant increase in the number of BrdU-labeled cells in dentate gyrus subgranular zone and hilus in APP/PS1 mice. These results suggest that BMP4 and Noggin co-modulate neurogenesis.

  11. Comparative mapping of DNA markers from the familial Alzheimer disease and Down syndrome regions of human chromosome 21 to mouse chromosomes 16 and 17

    SciTech Connect (OSTI)

    Cheng, S.V.; Nadeau, J.H.; Tanzi, R.E.; Watkins, P.C.; Jagadesh, J.; Taylor, B.A.; Haines, J.L.; Sacchi, N.; Gusella, J.F. )

    1988-08-01

    Mouse trisomy 16 has been proposed as an animal model of Down syndrome (DS), since this chromosome contains homologues of several loci from the q22 band of human chromosome 21. The recent mapping of the defect causing familial Alzheimer disease (FAD) and the locus encoding the Alzheimer amyloid {beta} precursor protein (APP) to human chromosome 21 has prompted a more detailed examination of the extent of conservation of this linkage group between the two species. Using anonymous DNA probes and cloned genes from human chromosome 21 in a combination of recombinant inbred and interspecific mouse backcross analyses, the authors have established that the linkage group shared by mouse chromosome 16 includes not only the critical DS region of human chromosome 21 but also the APP gene and FAD-linked markers. Extending from the anonymous DNA locus D21S52 to ETS2, the linkage map of six loci spans 39% recombination in man but only 6.4% recombination in the mouse. A break in synteny occurs distal to ETS2, with the homologue of the human marker D21S56 mapping to mouse chromosome 17. Conservation of the linkage relationships of markers in the FAD region suggests that the murine homologue of the FAD locus probably maps to chromosome 16 and that detailed comparison of the corresponding region in both species could facilitate identification of the primary defect in this disorder. The break in synteny between the terminal portion of human chromosome 21 and mouse chromosome 16 indicates, however, that mouse trisomy 16 may not represent a complete model of DS.

  12. Beta-Amyloid Deposition and Alzheimer's Type Changes Induced by Borrelia Spirochetes

    SciTech Connect (OSTI)

    Miklossy,J.; Kis, A.; Radenovic, A.; Miller, L.; Forro, L.; Martins, R.; Reiss, K.; Darbinian, N.; Darekar, P.; et al.

    2006-01-01

    The pathological hallmarks of Alzheimer's disease (AD) consist of {beta}-amyloid plaques and neurofibrillary tangles in affected brain areas. The processes, which drive this host reaction are unknown. To determine whether an analogous host reaction to that occurring in AD could be induced by infectious agents, we exposed mammalian glial and neuronal cells in vitro to Borrelia burgdorferi spirochetes and to the inflammatory bacterial lipopolysaccharide (LPS). Morphological changes analogous to the amyloid deposits of AD brain were observed following 2-8 weeks of exposure to the spirochetes. Increased levels of {beta}-amyloid presursor protein (A{beta}PP) and hyperphosphorylated tau were also detected by Western blots of extracts of cultured cells that had been treated with spirochetes or LPS. These observations indicate that, by exposure to bacteria or to their toxic products, host responses similar in nature to those observed in AD may be induced.

  13. A New F-18 Labeled PET Agent For Imaging Alzheimer's Plaques

    SciTech Connect (OSTI)

    Kulkarni, Padmakar V.; Hao Guiyang; Arora, Veera; Long, Michael; Slavine, Nikolai; Chiguru, Srinivas; Qu Baoxi; Sun Xiankai; Bennett, Michael; Antich, Peter P.; Bonte, Frederick J.; Vasdev, Neil

    2011-06-01

    Amyloid plaques and neurofibrillary tangles are hallmarks of Alzheimer's disease (AD). Advances in development of imaging agents have focused on targeting amyloid plaques. Notable success has been the development of C-11 labeled PIB (Pittsburgh Compound) and a number of studies have demonstrated the utility of this agent. However, the short half life of C-11 (t1/2: 20 min), is a limitation, thus has prompted the development of F-18 labeled agents. Most of these agents are derivatives of amyloid binding dyes; Congo red and Thioflavin. Some of these agents are in clinical trials with encouraging results. We have been exploring new class of agents based on 8-hydroxy quinoline, a weak metal chelator, targeting elevated levels of metals in plaques. Iodine-123 labeled clioquinol showed affinity for amyloid plaques however, it had limited brain uptake and was not successful in imaging in intact animals and humans. We have been successful in synthesizing F-18 labeled 8-hydroxy quinoline. Small animal PET/CT imaging studies with this agent showed high (7-10% ID/g), rapid brain uptake and fast washout of the agent from normal mice brains and delayed washout from transgenic Alzheimer's mice. These promising results encouraged us in further evaluation of this class of compounds for imaging AD plaques.

  14. Multimodal Imaging of Alzheimer Pathophysiology in the Brain's Default Mode Network

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Shin, Jonghan; Kepe, Vladimir; Small, Gary W.; Phelps, Michael E.; Barrio, Jorge R.

    2011-01-01

    The spatial correlations between the brain's default mode network (DMN) and the brain regions known to develop pathophysiology in Alzheimer's disease (AD) have recently attracted much attention. In this paper, we compare results of different functional and structural imaging modalities, including MRI and PET, and highlight different patterns of anomalies observed within the DMN. Multitracer PET imaging in subjects with and without dementia has demonstrated that [C-11]PIB- and [F-18]FDDNP-binding patterns in patients with AD overlap within nodes of the brain's default network including the prefrontal, lateral parietal, lateral temporal, and posterior cingulate cortices, with the exception of the medial temporalmore » cortex (especially, the hippocampus) where significant discrepancy between increased [F-18]FDDNP binding and negligible [C-11]PIB-binding was observed. [F-18]FDDNP binding in the medial temporal cortex—a key constituent of the DMN—coincides with both the presence of amyloid and tau pathology, and also with cortical areas with maximal atrophy as demonstrated by T1-weighted MR imaging of AD patients.« less

  15. OSTIblog Articles in the disease Topic | OSTI, US Dept of Energy Office of

    Office of Scientific and Technical Information (OSTI)

    Scientific and Technical Information disease Topic Sharing Data Leads to Progress by Nena Moss 19 Aug, 2010 in Personal Perspectives My mother died in March 2010 after a 15-year battle with Alzheimer's, so I pay particular attention to news about this dreadful disease. A recent New York Times article caught my eye: "Sharing of Data Leads to Progress on Alzheimer's." How did sharing data lead to progress on Alzheimer's? A collaborative effort, the Alzheimer's Disease Neuroimaging

  16. Ad Lucem Workshop Welcome

    Broader source: Energy.gov [DOE]

    This presentation summarizes the information given by DOE during the DOE Ad Lucem workshop, Feb. 17, 2012.

  17. Retiree AD&D Insurance

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AD&D Insurance Retiree AD&D Insurance AD&D coverage during retirement. Contact Retiree Insurance Providers Accidental death & dismemberment (AD&D) benefits for retirees The AD&D...

  18. Ad Lucem Workshop Welcome

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    I was a sorry witness of such doings, knowing that a little theory and calculation would have saved him ninety per cent of his labor." - Nikola Tesla (1931) Ad Lucem Modeling ...

  19. Fermilab Today - Classified Ad Policy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Fermilab Classified Ad Policy Fermilab Today now requires that people placing classified ads must belong to one of these categories: a. active and retired employees of Fermi...

  20. Kaolin AD | Open Energy Information

    Open Energy Info (EERE)

    Kaolin AD Jump to: navigation, search Name: Kaolin AD Place: Senovo, Bulgaria Zip: 7038 Sector: Solar Product: Kaolin is Europe's fourth largest producer of silica sands and owns a...

  1. Ad Hoc Meetings | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Ad Hoc Meetings Ad Hoc Meetings Ad Hoc Meetings 43.pdf (44.36 KB) More Documents & Publications Radio and Spectrum Management ICAM Workshop PKI Workshop

  2. Fermilab Today | Classifieds Ad Form

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    will be posted on the Internet and can lead to responses from outside the lab. Some ads posted in Fermilab Today in the past have received responses from scammers who, for...

  3. Gravitational charges of transverse asymptotically AdS spacetimes

    SciTech Connect (OSTI)

    Cebeci, Hakan; Sarioglu, Oezguer; Tekin, Bayram

    2006-12-15

    Using Killing-Yano symmetries, we construct conserved charges of spacetimes that asymptotically approach to the flat or anti-de Sitter spaces only in certain directions. In D dimensions, this allows one to define gravitational charges (such as mass and angular momenta densities) of p-dimensional branes/solitons or any other extended objects that curve the transverse space into an asymptotically flat or AdS one. Our construction answers the question of what kind of charges the antisymmetric Killing-Yano tensors lead to.

  4. A State-Wide Research Network for Alzheimer's Disease (Technical...

    Office of Scientific and Technical Information (OSTI)

    Contributing Orgs: MUSC & Ralph H. Johnson VAMC, Charleston, SC Senior Primary Care, Columbia, SC Comprehensive Neurological Services, Florence, SC Hospital Medicine Consultants, ...

  5. Systematics of Coupling Flows in AdS Backgrounds

    SciTech Connect (OSTI)

    Goldberger, Walter D.; Rothstein, Ira Z.

    2003-03-18

    We give an effective field theory derivation, based on the running of Planck brane gauge correlators, of the large logarithms that arise in the predictions for low energy gauge couplings in compactified AdS}_5 backgrounds, including the one-loop effects of bulk scalars, fermions, and gauge bosons. In contrast to the case of charged scalars coupled to Abelian gauge fields that has been considered previously in the literature, the one-loop corrections are not dominated by a single 4D Kaluza-Klein mode. Nevertheless, in the case of gauge field loops, the amplitudes can be reorganized into a leading logarithmic contribution that is identical to the running in 4D non-Abelian gauge theory, and a term which is not logarithmically enhanced and is analogous to a two-loop effect in 4D. In a warped GUT model broken by the Higgs mechanism in the bulk,we show that the matching scale that appears in the large logarithms induced by the non-Abelian gauge fields is m_{XY}^2/k where m_{XY} is the bulk mass of the XY bosons and k is the AdS curvature. This is in contrast to the UV scale in the logarithmic contributions of scalars, which is simply the bulk mass m. Our results are summarized in a set of simple rules that can be applied to compute the leading logarithmic predictions for coupling constant relations within a given warped GUT model. We present results for both bulk Higgs and boundary breaking of the GUT gauge

  6. The Ad Lucem Research Network

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The Ad Lucem Research Network Lada Adamic Associate Professor, School of Information & Center for the Study of Complex Systems University of Michigan Research interests: structure and dynamics of social and information networks, with a particular emphasis on information diffusion, expertise sharing, and online communities D. Lazer et al. "Computational Social Science." Science 323, 5915 (2009). J. Leskovec, L. A. Adamic, and B. A. Huberman. "The dynamics of viral

  7. OSTIblog Articles in the Alzheimer's Topic | OSTI, US Dept of...

    Office of Scientific and Technical Information (OSTI)

    John Q. Trojanowski, an Alzheimer's researcher at the University of Pennsylvania, "It's not science the way most of us have practiced it in our careers. But we all realized that we ...

  8. Doppler Lidar Wind Value-Added Product

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    8 Doppler Lidar Wind Value-Added Product RK Newsom C Sivaraman TR Shippert LD Riihimaki ... DOESC-ARMTR-148 Doppler Lidar Wind Value-Added Product Version 1.0 RK Newsom C Sivaraman ...

  9. Ad Lucem: Modeling Market Transformation Pathways Workshop

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AD LUCEM ... TOWARD THE LIGHT! 1 Ad Lucem: Modeling Market Transformation Pathways Workshop Berkeley, California | Friday, February 17, 2012 Ad lucem - Latin for toward the light - captures the essence of the SunShot Initiative. We seek to accelerate the global transition to clean, abundant solar energy by expanding our understanding of the technology-society-economics nexus. The ability to control energy resources is the enabler for growth, development, and opportunity. The diffusion of

  10. Ad-Venta | Open Energy Information

    Open Energy Info (EERE)

    190 alle de Bourgogne Place: Bourg de Page Country: France Zip: 26300 Sector: Hydrogen Product: Hydrogen storage (on board) Year Founded: 2006 Website: www.ad-venta.com...

  11. Transportation Plan Ad Hoc Working Group | Department of Energy

    Office of Environmental Management (EM)

    Plan Ad Hoc Working Group Transportation Plan Ad Hoc Working Group PDF icon Transportation Plan Ad Hoc Working Group More Documents & Publications Nuclear Fuel Storage and...

  12. Stability of Hořava-Lifshitz black holes in the context of AdS...

    Office of Scientific and Technical Information (OSTI)

    Stability of Hoava-Lifshitz black holes in the context of AdSCFT Citation Details In-Document Search Title: Stability of Hoava-Lifshitz black holes in the context of AdSCFT ...

  13. ARM - Value-Added Product Status Reports

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Status Reports Publications Journal Articles Conference Documents Program Documents & Technical Reports Publications Database Public Information Materials Image Library Videos Publication Resources Submit a Publication Publishing Procedures ARM Style Guide (PDF, 448KB) Acronyms Glossary Logos Contacts RSS for Publications Value-Added Product Status Reports ARM Climate Research Facility Quarterly Value-Added Product Report July 1-September 30, 2015 (PDF, 1MB) ARM Climate Research Facility

  14. Reveal for Adding OpenMP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reveal for Adding OpenMP Reveal for Adding OpenMP Reveal is an integrated performance analysis and code optimization tool. Reveal provides: Source code navigation using the analysis data of the entire program Performance data collected during program execution Dependency information for targeted loops Helps users in optimizing code by providing variable scoping feedback and suggested compiler directives For information on how to use Cray Reveal and how to use it on NERSC systems, refer to the

  15. QCD Condensates and Holographic Wilson Loops for Asymptotically AdS Spaces

    SciTech Connect (OSTI)

    Quevedo, R. Carcasses; Goity, Jose L.; Trinchero, Roberto C.

    2014-02-01

    The minimization of the Nambu-Goto (NG) action for a surface whose contour defines a circular Wilson loop of radius a placed at a finite value of the coordinate orthogonal to the border is considered. This is done for asymptotically AdS spaces. The condensates of dimension n = 2, 4, 6, 8, and 10 are calculated in terms of the coefficients in the expansion in powers of the radius a of the on-shell subtracted NG action for small a->0. The subtraction employed is such that it presents no conflict with conformal invariance in the AdS case and need not introduce an additional infrared scale for the case of confining geometries. It is shown that the UV value of the gluon condensates is universal in the sense that it only depends on the first coefficients of the difference with the AdS case.

  16. Ad Lucem: Modeling of Market Transformation Pathways Workshop...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Ad Lucem: Modeling of Market Transformation Pathways Workshop Agenda This document details the agenda for the DOE Ad Lucem: Modeling of Market Transformation Pathways Workshop on ...

  17. Building America Expert Meeting: Key Innovations for Adding Energy...

    Energy Savers [EERE]

    Key Innovations for Adding Energy Efficiency to Maintenance Projects Building America Expert Meeting: Key Innovations for Adding Energy Efficiency to Maintenance Projects This ...

  18. ARM Climate Research Facility Quarterly Value-Added Product Report...

    Office of Scientific and Technical Information (OSTI)

    Program Document: ARM Climate Research Facility Quarterly Value-Added Product Report Citation Details In-Document Search Title: ARM Climate Research Facility Quarterly Value-Added ...

  19. ARM Climate Research Facility Quarterly Value-Added Product Report...

    Office of Scientific and Technical Information (OSTI)

    ARM Climate Research Facility Quarterly Value-Added Product Report Citation Details In-Document Search Title: ARM Climate Research Facility Quarterly Value-Added Product Report ...

  20. ARM Climate Research Facility Quarterly Value-Added Product Report...

    Office of Scientific and Technical Information (OSTI)

    Technical Report: ARM Climate Research Facility Quarterly Value-Added Product Report Citation Details In-Document Search Title: ARM Climate Research Facility Quarterly Value-Added ...

  1. Shuchi Energy Ad Promotions P Ltd | Open Energy Information

    Open Energy Info (EERE)

    Shuchi Energy Ad Promotions P Ltd Jump to: navigation, search Name: Shuchi Energy Ad Promotions (P) Ltd Place: Hyderabad, Andhra Pradesh, India Zip: 500062 Sector: Solar Product:...

  2. The Ad Lucem Research Network | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ...lucem2012attendees.pdf More Documents & Publications Big Data Projects on Solar Tech Evolution and Diffusion Ad Lucem Workshop Welcome Ad Lucem Workshop: Modeling Breakout Session

  3. Addendum Added to Innovative Demonstration of Geothermal Energy...

    Energy Savers [EERE]

    Addendum Added to Innovative Demonstration of Geothermal Energy Production FOA Addendum Added to Innovative Demonstration of Geothermal Energy Production FOA July 2, 2010 - 2:13pm ...

  4. Supporting Dynamic Ad hoc Collaboration Capabilities

    SciTech Connect (OSTI)

    Agarwal, Deborah A.; Berket, Karlo

    2003-07-14

    Modern HENP experiments such as CMS and Atlas involve as many as 2000 collaborators around the world. Collaborations this large will be unable to meet often enough to support working closely together. Many of the tools currently available for collaboration focus on heavy-weight applications such as videoconferencing tools. While these are important, there is a more basic need for tools that support connecting physicists to work together on an ad hoc or continuous basis. Tools that support the day-to-day connectivity and underlying needs of a group of collaborators are important for providing light-weight, non-intrusive, and flexible ways to work collaboratively. Some example tools include messaging, file-sharing, and shared plot viewers. An important component of the environment is a scalable underlying communication framework. In this paper we will describe our current progress on building a dynamic and ad hoc collaboration environment and our vision for its evolution into a HENP collaboration environment.

  5. ARM - Value-Added Product (VAP) Reports

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (VAP) Reports Publications Journal Articles Conference Documents Program Documents & Technical Reports Publications Database Public Information Materials Image Library Videos Publication Resources Submit a Publication Publishing Procedures ARM Style Guide (PDF, 448KB) Acronyms Glossary Logos Contacts RSS for Publications Value-Added Product (VAP) Reports For proper viewing, technical reports should be viewed with Adobe Acrobat Reader. Download the latest version from the Adobe Reader

  6. ARM - Value-Added Products - Status

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    - Status Report Expand Orange | Expand Blue | Expand Green | Collapse All See Legend for Data Availability explanation. ARM - Value-Added Products - Status Last Update: March 21 2016 19:00:50 +/- Vap Name Translator Developer Frequency Tier Producer Data Availability ACRED (ARM Cloud Retrieval Ensemble Data) Shaocheng Xie Chuanfeng Zhao, Renata Mc Coy Periodically Evaluation Developer ARM Overview: Developer Description: The ARM Cloud Retrieval Ensemble Dataset (ACRED) is a multi-year cloud

  7. Merged Sounding Value-Added Product

    SciTech Connect (OSTI)

    Troyan, D

    2010-03-03

    The Merged Sounding value-added product (VAP) uses a combination of observations from radiosonde soundings, the microwave radiometer (MWR), surface meteorological instruments, and European Centre for Medium-Range Weather Forecasts (ECMWF) model output with a sophisticated scaling/interpolation/smoothing scheme in order to define profiles of the atmospheric thermodynamic state at one-minute temporal intervals and a total of 266 altitude levels.

  8. ARM KAZR-ARSCL Value Added Product

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Jensen, Michael

    The Ka-band ARM Zenith Radars (KAZRs) have replaced the long-serving Millimeter Cloud Radars, or MMCRs. Accordingly, the primary MMCR Value Added Product (VAP), the Active Remote Sensing of CLouds (ARSCL) product, is being replaced by a KAZR-based version, the KAZR-ARSCL VAP. KAZR-ARSCL provides cloud boundaries and best-estimate time-height fields of radar moments.

  9. ARM KAZR-ARSCL Value Added Product

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Jensen, Michael

    2012-09-28

    The Ka-band ARM Zenith Radars (KAZRs) have replaced the long-serving Millimeter Cloud Radars, or MMCRs. Accordingly, the primary MMCR Value Added Product (VAP), the Active Remote Sensing of CLouds (ARSCL) product, is being replaced by a KAZR-based version, the KAZR-ARSCL VAP. KAZR-ARSCL provides cloud boundaries and best-estimate time-height fields of radar moments.

  10. Association between environmental exposure to pesticides and neurodegenerative diseases

    SciTech Connect (OSTI)

    Parron, Tesifon; Requena, Mar; Hernandez, Antonio F.; Alarcon, Raquel

    2011-11-15

    Preliminary studies have shown associations between chronic pesticide exposure in occupational settings and neurological disorders. However, data on the effects of long-term non-occupational exposures are too sparse to allow any conclusions. This study examines the influence of environmental pesticide exposure on a number of neuropsychiatric conditions and discusses their underlying pathologic mechanisms. An ecological study was conducted using averaged prevalence rates of Alzheimer's disease, Parkinson's disease, multiple sclerosis, cerebral degeneration, polyneuropathies, affective psychosis and suicide attempts in selected Andalusian health districts categorized into areas of high and low environmental pesticide exposure based on the number of hectares devoted to intensive agriculture and pesticide sales per capita. A total of 17,429 cases were collected from computerized hospital records (minimum dataset) between 1998 and 2005. Prevalence rates and the risk of having Alzheimer's disease, Parkinson's disease, multiple sclerosis and suicide were significantly higher in districts with greater pesticide use as compared to those with lower pesticide use. The multivariate analyses showed that the population living in areas with high pesticide use had an increased risk for Alzheimer's disease and suicide attempts and that males living in these areas had increased risks for polyneuropathies, affective disorders and suicide attempts. In conclusion, this study supports and extends previous findings and provides an indication that environmental exposure to pesticides may affect the human health by increasing the incidence of certain neurological disorders at the level of the general population. -- Highlights: Black-Right-Pointing-Pointer Environmental exposure to pesticides and neurodegenerative-psychiatric disorders. Black-Right-Pointing-Pointer Increased risk for Alzheimer's disease and suicide attempts in high exposure areas. Black-Right-Pointing-Pointer Males from

  11. Entropy of near-extremal black holes in AdS5

    SciTech Connect (OSTI)

    Simon, Joan; Balasubramanian, Vijay; de Boer, Jan; Jejjala, Vishnu; Simon, Joan

    2007-07-24

    We construct the microstates of near-extremal black holes in AdS_5 x S5 as gases of defects distributed in heavy BPS operators in the dual SU(N) Yang-Mills theory. These defects describe open strings on spherical D3-branes in the S5, and we show that they dominate the entropy by directly enumerating them and comparing the results with a partition sum calculation. We display new decoupling limits in which the field theory of the lightest open strings on the D-branes becomes dual to a near-horizon region of the black hole geometry. In the single-charge black hole we find evidence for an infrared duality between SU(N) Yang-Mills theories that exchanges the rank of the gauge group with an R-charge. In the two-charge case (where pairs of branes intersect on a line), the decoupled geometry includes an AdS_3 factor with a two-dimensional CFT dual. The degeneracy in this CFT accounts for the black hole entropy. In the three-charge case (where triples of branes intersect at a point), the decoupled geometry contains an AdS_2 factor. Below a certain critical mass, the two-charge system displays solutions with naked timelike singularities even though they do not violate a BPS bound. We suggest a string theoretic resolution of these singularities.

  12. Interpolated Sounding Value-Added Product

    SciTech Connect (OSTI)

    Troyan, D

    2013-04-01

    The Interpolated Sounding (INTERPSONDE) value-added product (VAP) uses a combination of observations from radiosonde soundings, the microwave radiometer (MWR), and surface meteorological instruments in order to define profiles of the atmospheric thermodynamic state at one-minute temporal intervals and a total of at least 266 altitude levels. This VAP is part of the Merged Sounding (MERGESONDE) suite of VAPs. INTERPSONDE is the profile of the atmospheric thermodynamic state created using the algorithms of MERGESONDE without including the model data from the European Centre for Medium-range Weather Forecasting (ECMWF). More specifically, INTERPSONDE VAP represents an intermediate step within the larger MERGESONDE process.

  13. Ad Lucem: Modeling of Market Transformation Pathways Workshop Agenda |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy of Market Transformation Pathways Workshop Agenda Ad Lucem: Modeling of Market Transformation Pathways Workshop Agenda This document details the agenda for the DOE Ad Lucem: Modeling of Market Transformation Pathways Workshop on Feb. 17, 2012. adlucem2012_agenda.pdf (295.01 KB) More Documents & Publications Ad Lucem Workshop Welcome Ad Lucem: Modeling Market Transformation Pathways Workshop The Ad Lucem Research Network

  14. Adding Insulation to an Existing Home | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Weatherize » Insulation » Adding Insulation to an Existing Home Adding Insulation to an Existing Home Adding insulation in an existing home saves money and improves comfort. | Photo courtesy of Dennis Schroeder, NREL. Adding insulation in an existing home saves money and improves comfort. | Photo courtesy of Dennis Schroeder, NREL. Unless your home was specially constructed for energy efficiency, you can probably reduce your energy bills by adding more insulation. Many older homes have less

  15. POSSIBLE CHROMOSPHERIC ACTIVITY CYCLES IN AD LEO

    SciTech Connect (OSTI)

    Buccino, Andrea P.; Petrucci, Romina; Mauas, Pablo J. D.; Jofr, Emiliano

    2014-01-20

    AD Leo (GJ388) is an active dM3 flare star that has been extensively observed both in the quiescent and flaring states. Since this active star is near the fully convective boundary, studying its long-term chromospheric activity in detail could be an appreciable contribution to dynamo theory. Here, using the Lomb-Scargle periodogram, we analyze the Ca IIK line-core fluxes derived from CASLEO spectra obtained between 2001 and 2013 and the V magnitude from the ASAS database between 2004 and 2010. From both of these totally independent time series, we obtain a possible activity cycle with a period of approximately seven years and a less significant shorter cycle of approximately two years. A tentative interpretation is that a dynamo operating near the surface could be generating the longer cycle, while a second dynamo operating in the deep convection zone could be responsible for the shorter one. Based on the long duration of our observing program at CASLEO and the fact that we observe different spectral features simultaneously, we also analyze the relation between simultaneous measurements of the Na I index (R{sub D}{sup ?}), H?, and Ca IIK fluxes at different activity levels of AD Leo, including flares.

  16. Quantum compositeness of gravity: black holes, AdS and inflation

    SciTech Connect (OSTI)

    Dvali, Gia; Gomez, Cesar E-mail: cesar.gomez@uam.es

    2014-01-01

    Gravitational backgrounds, such as black holes, AdS, de Sitter and inflationary universes, should be viewed as composite of N soft constituent gravitons. It then follows that such systems are close to quantum criticality of graviton Bose-gas to Bose-liquid transition. Generic properties of the ordinary metric description, including geodesic motion or particle-creation in the background metric, emerge as the large-N limit of quantum scattering of constituent longitudinal gravitons. We show that this picture correctly accounts for physics of large and small black holes in AdS, as well as reproduces well-known inflationary predictions for cosmological parameters. However, it anticipates new effects not captured by the standard semi-classical treatment. In particular, we predict observable corrections that are sensitive to the inflationary history way beyond last 60 e-foldings. We derive an absolute upper bound on the number of e-foldings, beyond which neither de Sitter nor inflationary Universe can be approximated by a semi-classical metric. However, they could in principle persist in a new type of quantum eternity state. We discuss implications of this phenomenon for the cosmological constant problem.

  17. Quantum compositeness of gravity: black holes, AdS and inflation

    SciTech Connect (OSTI)

    Dvali, Gia; Gomez, Cesar

    2014-01-14

    Gravitational backgrounds, such as black holes, AdS, de Sitter and inflationary universes, should be viewed as composite of N soft constituent gravitons. It then follows that such systems are close to quantum criticality of graviton Bose-gas to Bose-liquid transition. Generic properties of the ordinary metric description, including geodesic motion or particle-creation in the background metric, emerge as the large-N limit of quantum scattering of constituent longitudinal gravitons. We show that this picture correctly accounts for physics of large and small black holes in AdS, as well as reproduces well-known inflationary predictions for cosmological parameters. However, it anticipates new effects not captured by the standard semi-classical treatment. In particular, we predict observable corrections that are sensitive to the inflationary history way beyond last 60 e-foldings. We derive an absolute upper bound on the number of e-foldings, beyond which neither de Sitter nor inflationary Universe can be approximated by a semi-classical metric. However, they could in principle persist in a new type of quantum eternity state. We discuss implications of this phenomenon for the cosmological constant problem.

  18. AD Hydro Power Ltd ADHPL | Open Energy Information

    Open Energy Info (EERE)

    AD Hydro Power Ltd ADHPL Jump to: navigation, search Name: AD Hydro Power Ltd. (ADHPL) Place: Noida, Uttar Pradesh, India Zip: 201301 Sector: Hydro Product: Noida-based small hydro...

  19. Ad Lucem Workshop: Modeling Breakout Session | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Workshop: Modeling Breakout Session Ad Lucem Workshop: Modeling Breakout Session This presentation summarizes the information discussed during the modeling breakout session of the DOE Ad Lucem Workshop on Feb. 17, 2012. adlucem2012_modeling_breakout.pdf (1.66 MB) More Documents & Publications Ad Lucem: Modeling of Market Transformation Pathways Workshop Agenda Ad Lucem: Modeling Market Transformation Pathways Workshop Break-out Discussion i: Modeling Consumer Behavior Residential S

  20. Ad Lucem: Modeling Market Transformation Pathways Workshop | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Market Transformation Pathways Workshop Ad Lucem: Modeling Market Transformation Pathways Workshop This white paper summarizes the information discussed during the Ad Lucem: Modeling Market Transformation Pathways Workshop, Feb. 17, 2012, in Berkeley, California. adlucem2012_whitepaper.pdf (547.71 KB) More Documents & Publications Ad Lucem: Modeling of Market Transformation Pathways Workshop Agenda Ad Lucem Workshop Welcome Materials from 2014 SunShot Summit BREAKOUT SESSION: THE

  1. Ads-tec GmbH | Open Energy Information

    Open Energy Info (EERE)

    Raiffeisenstrasse 14 Place: Leinfelden Echterdingen Zip: 70771 Sector: Services Product: Energy Storage Systems Number of Employees: 51-200 Website: www.ads-tec.de Coordinates:...

  2. Recently Added or Revised "Slice" Information (contracts/slice...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Product Recently Added or Revised Information (updated November 2, 2011) Applicable Interest Rates for Slice Product (updated November 2, 2011) Slice Product Review Meeting...

  3. ARM Climate Research Facility Quarterly Value-Added Product Report...

    Office of Scientific and Technical Information (OSTI)

    Climate Research Facility Quarterly Value-Added Product Report Fourth Quarter: July 1-September 30, 2012 Citation Details In-Document Search Title: ARM Climate Research Facility ...

  4. Adding Insulation to an Existing Home | Department of Energy

    Office of Environmental Management (EM)

    existing home saves money and improves comfort. | Photo courtesy of Dennis Schroeder, NREL. Adding insulation in an existing home saves money and improves comfort. | Photo...

  5. Bioenergy Demonstration Project: Value-Added Products from Renewable...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bioenergy Demonstration Project: Value-Added Products from Renewable Fuels May 23, 2013 Technology Area Review: Biochemical Conversion Paul Blum University of Nebraska 2 Goal ...

  6. ARM Climate Research Facility Quarterly Value-Added Product Report

    SciTech Connect (OSTI)

    Sivaraman, Chitra

    2014-11-21

    The purpose of this report is to provide a concise status update for value-added products (VAP) implemented by the Atmospheric Radiation Measurement (ARM) Climate Research Facility.

  7. DOE Announces Webinars on Adding Solar to Your Building Portfolio...

    Office of Environmental Management (EM)

    DOE Announces Webinars on Adding Solar to Your Building ... from adopting the latest energy efficiency and renewable ... January 28: How to Utilize the National Geothermal Data ...

  8. Added Public Promotional Page! | OpenEI Community

    Open Energy Info (EERE)

    Added Public Promotional Page Home > Groups > Linked Open Data Workshop in Washington, D.C. Jweers's picture Submitted by Jweers(88) Contributor 27 September, 2012 - 18:10 Event...

  9. Adding Nanocavities to Catalyst Surfaces Enhances Chemical Selectivity...

    Office of Science (SC) Website

    made by adding a template during the atomic layer deposition process that is used to ... Publications C. P. Canlas, J. Lu, N. A. Ray, N. A. Grosso-Giordano, J. W. Elam, S. Lee, R. ...

  10. Doppler Lidar Vertical Velocity Statistics Value-Added Product

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    49 Doppler Lidar Vertical Velocity Statistics Value-Added Product RK Newsom C Sivaraman TR Shippert LD Riihimaki July 2015 DISCLAIMER This report was prepared as an account of work...

  11. Value-added agriculture offers small agribusinesses additional income

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    potential Value-added agriculture Community Connections: Your link to news and opportunities from Los Alamos National Laboratory Latest Issue: September 1, 2016 all issues All Issues » submit Value-added agriculture offers small agribusinesses additional income potential Closing the gap between raw product and end user September 1, 2015 Las Nueve Niñas Winery wine label. Las Nueve Niñas Winery wine label. Contact Community Programs Director Kathy Keith Email Editor Ute Haker Email Instead

  12. Anaerobic Digestion (AD): not only methane | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Anaerobic Digestion (AD): not only methane Anaerobic Digestion (AD): not only methane Breakout Session 1: New Developments and Hot Topics Session 1-C: Beyond Biofuels Larry Baresi, Professor of Biology, California State University, Northridge b13_baresi_1-C.pdf (980.78 KB) More Documents & Publications Electrobiocommodities from Carbon Dioxide: Enhancing Microbial Electrosynthesis with Synthetic Electromicrobiology and System Design Hydrogen, Hydrocarbons, and Bioproduct Precursors from

  13. Comments on Cosmic Censorship in AdS/CFT

    SciTech Connect (OSTI)

    Hubeny, Veronika E.; Liu, Xiao; Rangamani, Mukund; Shenker, Stephen

    2004-03-21

    Recently Hertog, Horowitz, and Maeda (HHM) (hep-th/0310054) have proposed that cosmic censorship can be violated in the AdS/CFT context. They argue that for certain initial data there is insufficient energy available to make a black hole whose horizon is big enough to cloak the singularity that forms. We have investigated this proposal in the models HHM discuss and have thus far been unable to find initial data that provably satisfy this criterion, despite our development of an improved lower bound on the size of the singular region. This is consistent with recent numerical results (hep-th/0402109). For certain initial data, the energies of our configurations are not far above the lower bound on the requisite black hole mass, and so it is possible that in the exact time development naked singularities do form. We go on to argue that the finite radius cut-off AdS_5 situation discussed by HHM displays instabilities when the full 10D theory is considered. We propose an AdS_3 example that may well be free of this instability.

  14. Comments on Cosmic Censorship in AdS/CFT

    SciTech Connect (OSTI)

    Hubeny, V.

    2004-04-06

    Recently Hertog, Horowitz, and Maeda (HHM) (hep-th/0310054) have proposed that cosmic censorship can be violated in the AdS/CFT context. They argue that for certain initial data there is insufficient energy available to make a black hole whose horizon is big enough to cloak the singularity that forms. We have investigated this proposal in the models HHM discuss and have thus far been unable to find initial data that provably satisfy this criterion, despite our development of an improved lower bound on the size of the singular region. This is consistent with recent numerical results (hep-th/0402109). For certain initial data, the energies of our configurations are not far above the lower bound on the requisite black hole mass, and so it is possible that in the exact time development naked singularities do form. We go on to argue that the finite radius cut-off AdS5 situation discussed by HHM displays instabilities when the full 10D theory is considered. We propose an AdS3 example that may well be free of this instability.

  15. No-carrier-added [1.sup.11 c]putrescine

    DOE Patents [OSTI]

    McPherson, Daniel W.; Fowler, Joanna S.; Wolf, Alfred P.

    1989-01-01

    The invention relates to a new radiolabeled imaging agent, no-carrier-added [1-.sup.11 C]putrescine, and to the use of this very pure material as a radiotracer with positron emission tomography for imaging brain tumors. The invention further relates to the synthesis of no-carrier-added [1-.sup.11 C]putrescine based on the Michael addition of potassium .sup.11 C-labeled cyanide to acrylonitrile followed by reduction of the .sup.11 C-labeled dinitrile. The new method is rapid and efficient and provides radiotracer with a specific activity greater than 1.4 curies per millimol and in a purity greater than 95%.

  16. Raman Lidar Profiles–Temperature (RLPROFTEMP) Value-Added Product

    SciTech Connect (OSTI)

    Newsom, RK; Sivaraman, C; McFarlane, SA

    2012-10-31

    The purpose of this document is to describe the Raman Lidar Profiles–Temperature (RLPROFTEMP) value-added product (VAP) and the procedures used to derive atmospheric temperature profiles from the raw RL measurements. Sections 2 and 4 describe the input and output variables, respectively. Section 3 discusses the theory behind the measurement and the details of the algorithm, including calibration and overlap correction.

  17. Top Value-Added Chemicals from Biomass - Volume II„Results of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Value-Added Chemicals from Biomass - Volume IIResults of Screening for Potential Candidates from Biorefinery Lignin Top Value-Added Chemicals from Biomass - Volume IIResults of ...

  18. Doppler Lidar Wind Value-Added Product (Technical Report) | SciTech...

    Office of Scientific and Technical Information (OSTI)

    Wind Value-Added Product Citation Details In-Document Search Title: Doppler Lidar Wind Value-Added Product Wind speed and direction, together with pressure, temperature, and ...

  19. Neutron Instruments Added at Oak Ridge (Journal Article) | SciTech...

    Office of Scientific and Technical Information (OSTI)

    Neutron Instruments Added at Oak Ridge Citation Details In-Document Search Title: Neutron Instruments Added at Oak Ridge The neutron scattering facilities at Oak Ridge National ...

  20. Evaluation of the Ad Council/DOE "What's Your Excuse" Public...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Ad CouncilDOE "What's Your Excuse" Public Service Advertising Campaign Evaluation of the Ad CouncilDOE "What's Your Excuse" Public Service Advertising Campaign Evaluation of the ...

  1. Organic Aerosol Component (OACOMP) Value-Added Product Report

    SciTech Connect (OSTI)

    Fast, J; Zhang, Q; Tilp, A; Shippert, T; Parworth, C; Mei, F

    2013-08-23

    Significantly improved returns in their aerosol chemistry data can be achieved via the development of a value-added product (VAP) of deriving OA components, called Organic Aerosol Components (OACOMP). OACOMP is primarily based on multivariate analysis of the measured organic mass spectral matrix. The key outputs of OACOMP are the concentration time series and the mass spectra of OA factors that are associated with distinct sources, formation and evolution processes, and physicochemical properties.

  2. Linear Fresnel Technology added to System Advisor Model's Capabilities -

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    News Releases | NREL Linear Fresnel Technology added to System Advisor Model's Capabilities Now utilities can get detailed information on siting, performance and finances February 8, 2012 A promising Concentrating Solar Power (CSP) technology that uses a stationary receiver tube and an array of mirrors mounted near the ground can now be accessed within the System Advisor Model (SAM), which predicts annual energy production, hourly performance and return on investment. The U.S. Department of

  3. New Atmospheric Profiling Instrument Added to SGP CART Suite

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    3 New Atmospheric Profiling Instrument Added to SGP CART Suite A new atmospheric profiling instrument at the SGP CART site is giving researchers an additional useful data stream. The new instrument is a microwave radiometer profiler (MWRP) developed by Radiometrics Corporation. One ARM Program focus is improving the quality of simulations by global climate models, particularly models that deal with interactions between sunlight (solar radiation) and clouds. To support this improvement, ARM needs

  4. ARM Climate Research Facility Quarterly Value-Added Product Report

    SciTech Connect (OSTI)

    Sivaraman, Chitra

    2013-07-31

    The purpose of this report is to provide a concise status update for value-added products (VAP) implemented by the Atmospheric Radiation Measurement Climate Research Facility. The report is divided into the following sections: (1) new VAPs for which development has begun, (2) progress on existing VAPs, (3) future VAPs that have been recently approved, (4) other work that leads to a VAP, and (5) top requested VAPs from the archive.

  5. 2D Gridded Surface Data Value-Added Product

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2 2D Gridded Surface Data Value-Added Product Q Tang S Xie July 2015 DISCLAIMER This report was prepared as an account of work sponsored by the U.S. Government. Neither the United States nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned

  6. A Natural Language for AdS/CFT Correlators

    SciTech Connect (OSTI)

    Fitzpatrick, A.Liam; Kaplan, Jared; Penedones, Joao; Raju, Suvrat; van Rees, Balt C.; /YITP, Stony Brook

    2012-02-14

    We provide dramatic evidence that 'Mellin space' is the natural home for correlation functions in CFTs with weakly coupled bulk duals. In Mellin space, CFT correlators have poles corresponding to an OPE decomposition into 'left' and 'right' sub-correlators, in direct analogy with the factorization channels of scattering amplitudes. In the regime where these correlators can be computed by tree level Witten diagrams in AdS, we derive an explicit formula for the residues of Mellin amplitudes at the corresponding factorization poles, and we use the conformal Casimir to show that these amplitudes obey algebraic finite difference equations. By analyzing the recursive structure of our factorization formula we obtain simple diagrammatic rules for the construction of Mellin amplitudes corresponding to tree-level Witten diagrams in any bulk scalar theory. We prove the diagrammatic rules using our finite difference equations. Finally, we show that our factorization formula and our diagrammatic rules morph into the flat space S-Matrix of the bulk theory, reproducing the usual Feynman rules, when we take the flat space limit of AdS/CFT. Throughout we emphasize a deep analogy with the properties of flat space scattering amplitudes in momentum space, which suggests that the Mellin amplitude may provide a holographic definition of the flat space S-Matrix.

  7. Process and targets for production of no-carrier-added radiotin

    DOE Patents [OSTI]

    Srivastava, Suresh C; Zhuikov, Boris Leonidovich; Ermolaev, Stanislav Victorovich; Konyakhin, Nikolay Alexandrovich; Kokhanyuk, Vladimir Mikhailovich; Khamyanov, Stepan Vladimirovich; Togaeva, Natalya Roaldovna

    2014-04-22

    One embodiment of the present invention includes a process for production and recovery of no-carrier-added radioactive tin (NCA radiotin). An antimony target can be irradiated with a beam of accelerated particles forming NCA radiotin, followed by separation of the NCA radiotin from the irradiated target. The target is metallic Sb in a hermetically sealed shell. The shell can be graphite, molybdenum, or stainless steel. The irradiated target can be removed from the shell by chemical or mechanical means, and dissolved in an acidic solution. Sb can be removed from the dissolved irradiated target by extraction. NCA radiotin can be separated from the remaining Sb and other impurities using chromatography on silica gel sorbent. NCA tin-117m can be obtained from this process. NCA tin-117m can be used for labeling organic compounds and biological objects to be applied in medicine for imaging and therapy of various diseases.

  8. Vocational Rehabilitation -Value Added: Explaining What We Do,

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Vocational Rehabilitation -Value Added: Explaining What We Do, Craig Bock, MA, CRC Washington State IARP Quarterly Newsletter - June 2009 If you have an injury at work, do you know what happens next or how you would navigate the Workers' Compensation system should you need to? What does RCW 51.32.095 (state law) and WAC 296-19A-070 (administrative rules) mean to you? If you could not return to your job and had permanent physical or cognitive restrictions who would help you explore your return to

  9. Commercial Demonstration of Wood Recovery, Recycling, and Value Adding Technologies

    SciTech Connect (OSTI)

    Auburn Machinery, Inc.

    2004-07-15

    This commercial demonstration project demonstrated the technical feasibility of converting low-value, underutilized and waste stream solid wood fiber material into higher valued products. With a growing need to increase product/production yield and reduce waste in most sawmills, few recovery operations and practically no data existed to support the viability of recovery operations. Prior to our efforts, most all in the forest products industry believed that recovery was difficult, extremely labor intensive, not cost effective, and that recovered products had low value and were difficult to sell. This project provided an opportunity for many within the industry to see through demonstration that converting waste stream material into higher valued products does in fact offer a solution. Our work, supported by the U.S. Department of Energy, throughout the project aimed to demonstrate a reasonable approach to reducing the millions of recoverable solid wood fiber tons that are annually treated as and converted into low value chips, mulch and fuel. Consequently sawmills continue to suffer from reduced availability of forest resources, higher raw material costs, growing waste disposal problems, increased global competition, and more pressure to operate in an Environmentally Friendly manner. It is our belief (based upon the experience of this project) that the successful mainstreaming of the recovery concept would assist in alleviating this burden as well as provide for a realistically achievable economic benefit to those who would seriously pursue the concept and tap into the rapidly growing ''GREEN'' building marketplace. Ultimately, with participation and aggressive pursuit of the recovery concept, the public would benefit in that: (1) Landfill/disposal waste volume could be reduced adding greater life to existing municipal landfill sites thereby minimizing the need to prematurely license and open added facilities. Also, there would be a cost avoidance benefit associated

  10. Some engineering properties of heavy concrete added silica fume

    SciTech Connect (OSTI)

    Akka?, Ay?e; Ba?yi?it, Celalettin; Esen, Serap

    2013-12-16

    Many different types of building materials have been used in building construction for years. Heavy concretes can be used as a building material for critical building as it can contain a mixture of many heavy elements. The barite itself for radiation shielding can be used and also in concrete to produce the workable concrete with a maximum density and adequate structural strength. In this study, some engineering properties like compressive strength, elasticity modules and flexure strength of heavy concretes added Silica fume have been investigated.

  11. Adding kinetics and hydrodynamics to the CHEETAH thermochemical code

    SciTech Connect (OSTI)

    Fried, L.E., Howard, W.M., Souers, P.C.

    1997-01-15

    In FY96 we released CHEETAH 1.40, which made extensive improvements on the stability and user friendliness of the code. CHEETAH now has over 175 users in government, academia, and industry. Efforts have also been focused on adding new advanced features to CHEETAH 2.0, which is scheduled for release in FY97. We have added a new chemical kinetics capability to CHEETAH. In the past, CHEETAH assumed complete thermodynamic equilibrium and independence of time. The addition of a chemical kinetic framework will allow for modeling of time-dependent phenomena, such as partial combustion and detonation in composite explosives with large reaction zones. We have implemented a Wood-Kirkwood detonation framework in CHEETAH, which allows for the treatment of nonideal detonations and explosive failure. A second major effort in the project this year has been linking CHEETAH to hydrodynamic codes to yield an improved HE product equation of state. We have linked CHEETAH to 1- and 2-D hydrodynamic codes, and have compared the code to experimental data. 15 refs., 13 figs., 1 tab.

  12. Matching the Hagedorn temperature in AdS/CFT correspondence

    SciTech Connect (OSTI)

    Harmark, Troels; Orselli, Marta

    2006-12-15

    We match the Hagedorn/deconfinement temperature of planar N=4 super Yang-Mills (SYM) on RxS{sup 3} to the Hagedorn temperature of string theory on AdS{sub 5}xS{sup 5}. The match is done in a near-critical region where both gauge theory and string theory are weakly coupled. The near-critical region is near a point with zero temperature and critical chemical potential. On the gauge-theory side we are taking a decoupling limit found in Ref. 7 in which the physics of planar N=4 SYM is given exactly by the ferromagnetic XXX{sub 1/2} Heisenberg spin chain. We find moreover a general relation between the Hagedorn/deconfinement temperature and the thermodynamics of the Heisenberg spin chain and we use this to compute it in two distinct regimes. On the string-theory side, we identify the dual limit for which the string tension and string coupling go to zero. This limit is taken of string theory on a maximally supersymmetric pp-wave background with a flat direction, obtained from a Penrose limit of AdS{sub 5}xS{sup 5}. We compute the Hagedorn temperature of the string theory and find agreement with the Hagedorn/deconfinement temperature computed on the gauge-theory side.

  13. 2D Gridded Surface Data Value-Added Product

    SciTech Connect (OSTI)

    Tang, Q; Xie, S

    2015-08-30

    This report describes the Atmospheric Radiation Measurement (ARM) Best Estimate (ARMBE) 2-dimensional (2D) gridded surface data (ARMBE2DGRID) value-added product. Spatial variability is critically important to many scientific studies, especially those that involve processes of great spatial variations at high temporal frequency (e.g., precipitation, clouds, radiation, etc.). High-density ARM sites deployed at the Southern Great Plains (SGP) allow us to observe the spatial patterns of variables of scientific interests. The upcoming megasite at SGP with its enhanced spatial density will facilitate the studies at even finer scales. Currently, however, data are reported only at individual site locations at different time resolutions for different datastreams. It is difficult for users to locate all the data they need and requires extra effort to synchronize the data. To address these problems, the ARMBE2DGRID value-added product merges key surface measurements at the ARM SGP sites and interpolates the data to a regular 2D grid to facilitate the data application.

  14. Zero added oxygen for high quality sputtered ITO. A data science investigation of reduced Sn-content and added Zr

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Peshek, Timothy J.; Burst, James M.; Coutts, Timothy J.; Gessert, Timothy A.

    2016-01-19

    Here, we demonstrate mobilities of >45 cm2/V s for sputtered tin-doped indium oxide (ITO) films at zero added oxygen. All films were deposited with 5 wt. % SnO2, instead of the more conventional 8–10 wt. %, and had varying ZrO2 content from 0 to 3 wt. %, with a subsequent reduction in In2O3 content. Moreover, these films were deposited by radio-frequency magnetron sputtering from nominally stoichiometric targets with varying oxygen partial pressure in the sputter ambient. Anomalous behavior was discovered for films with no Zr-added, where a bimodality of high and low mobilities was discovered for nominally similar growth conditions.more » However, all films showed the lowest resistivity and highest mobilities when the oxygen partial pressure in the sputter ambient was zero. This result is contrasted with several other reports of ITO transport performance having a maximum for small but nonzero oxygen partial pressure. Our result is attributed to the reduced concentration of SnO2. The addition of ZrO2 yielded the highest mobilities at >55 cm2/V s and the films showed a modest increase in optical transmission with increasing Zr-content.« less

  15. Perfect fluidity of a dissipative system: Analytical solution for the Boltzmann equation in AdS2 Ⓧ S2

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Noronha, Jorge; Denicol, Gabriel S.

    2015-12-30

    In this paper we obtain an analytical solution of the relativistic Boltzmann equation under the relaxation time approximation that describes the out-of-equilibrium dynamics of a radially expanding massless gas. This solution is found by mapping this expanding system in flat spacetime to a static flow in the curved spacetime AdS2 Ⓧ S2. We further derive explicit analytic expressions for the momentum dependence of the single-particle distribution function as well as for the spatial dependence of its moments. We find that this dissipative system has the ability to flow as a perfect fluid even though its entropy density does not matchmore » the equilibrium form. The nonequilibrium contribution to the entropy density is shown to be due to higher-order scalar moments (which possess no hydrodynamical interpretation) of the Boltzmann equation that can remain out of equilibrium but do not couple to the energy-momentum tensor of the system. Furthermore, in this system the slowly moving hydrodynamic degrees of freedom can exhibit true perfect fluidity while being totally decoupled from the fast moving, nonhydrodynamical microscopic degrees of freedom that lead to entropy production.« less

  16. No-carrier-added (/sup 18/F)-N-methylspiroperidol

    DOE Patents [OSTI]

    Shiue, C.Y.; Fowler, J.S.; Wolf, A.P.

    1985-10-04

    The present invention is directed to the synthesis of a radioligand, labeled with a positron emitting radionuclide which is suitable for dynamic studies in humans using positron emission transaxial tomography. No-carrier-added (NCA) (/sup 18/F)-N-methylspiroperiodl is prepared from four different sustrates: p-nitrobenzonitrile, cyclopropyl p-nitrophenyl ketone, p-cyclopropanoyl-N,N,N-trimethylanilinium iodide and p-cyclopropanoyl-N,N,N-trimethylanilinium perchlorate. The process for the production of NCA (/sup 18/F)-N-methylspiroperidol is a nucleophilic aromatic substitution reaction. Furthermore, the compound of this invention is shown to be effective as a new drug of choice for in vivo examination of dopamine binding sites in a human brain. In particular, this drug is primarily useful in the noninvasive technique of positron emission transaxial tomography (PETT).

  17. Doppler Lidar Vertical Velocity Statistics Value-Added Product

    SciTech Connect (OSTI)

    Newsom, R. K.; Sivaraman, C.; Shippert, T. R.; Riihimaki, L. D.

    2015-07-01

    Accurate height-resolved measurements of higher-order statistical moments of vertical velocity fluctuations are crucial for improved understanding of turbulent mixing and diffusion, convective initiation, and cloud life cycles. The Atmospheric Radiation Measurement (ARM) Climate Research Facility operates coherent Doppler lidar systems at several sites around the globe. These instruments provide measurements of clear-air vertical velocity profiles in the lower troposphere with a nominal temporal resolution of 1 sec and height resolution of 30 m. The purpose of the Doppler lidar vertical velocity statistics (DLWSTATS) value-added product (VAP) is to produce height- and time-resolved estimates of vertical velocity variance, skewness, and kurtosis from these raw measurements. The VAP also produces estimates of cloud properties, including cloud-base height (CBH), cloud frequency, cloud-base vertical velocity, and cloud-base updraft fraction.

  18. Stabilization of lead lithium iron tungstate with adding barium titanate

    SciTech Connect (OSTI)

    Lu, C.; Fang, B.

    1997-01-01

    The kinetics of formation, phase stabilities, and dielectric constants of Pb(Li{sub 1/4}Fe{sub 1/4}W{sub 1/2})O{sub 3} and BaTiO{sub 3}-added Pb(Li{sub 1/4}Fe{sub 1/4}W{sub 1/2})O{sub 3} have been compared. The addition of 2 mol{percent} BaTiO{sub 3} in Pb(Li{sub 1/4}Fe{sub 1/4}W{sub 1/2})O{sub 3} was confirmed to promote the complete formation of the perovskite phase at 700{degree}C. Also the thermal stability of the perovskite phase was significantly enhanced, which results in an increase of the dielectric permittivity. {copyright} {ital 1997 Materials Research Society.}

  19. Station-based Surface Data Value-Added Product

    SciTech Connect (OSTI)

    Tang, Q.; Xie, S.

    2015-07-01

    This report describes the Atmospheric Radiation Measurement (ARM) Best Estimate (ARMBE) station-based surface data (ARMBESTNS) value-added product. It is a twin data product of the ARMBE 2-Dimensional gridded (ARMBE2DGRID) data set. Unlike the ARMBE2DGRID data set, ARMBESTNS data are reported at the original site locations and show the original information (except for the interpolation over time). Therefore, the users have the flexibility to process the data with the approach more suitable for their applications. This document provides information about the input data, quality control (QC) method, and output format of this data set. As much of the information is identical to that of the ARMBE2DGRID data, this document will emphasize more on the different aspects of these two data sets.

  20. Sonde Adjust Value-Added Product Technical Report

    SciTech Connect (OSTI)

    Troyan, D

    2012-01-09

    The Sonde Adjust (SONDEADJUST) value-added product (VAP) creates a file that includes all fields from original Atmospheric Radiation Measurement Climate Research Facility (ARM Facility) radiosonde files and contains several value-added fields that provide adjustments related to well-known humidity issues. SONDEADJUST produces data that correct documented biases in radiosonde humidity measurements. Previous efforts towards applying some of these corrections are available via the discontinued PI product sgpsondecorr1miloC1. Unique fields contained within this datastream include smoothed original relative humidity, dry bias corrected relative humidity, and final corrected relative humidity. The smoothed RH field refines the relative humidity from integers-the resolution of the instrument-to fractions of a percent. This profile is then used to calculate the dry bias corrected field. The final correction fixes the time-lag problem and uses the dry-bias field as input into the algorithm. In addition to dry bias, solar heating is another correction that is encompassed in the final corrected RH field. Output from SONDEADJUST differs from the previous RH-corrected datastreams in important ways. First, all three types of ARM radiosondes-Vaisala RS-80, RS-90, and RS-92-are corrected using dedicated procedures and/or parameters. Second, the output variables include all of those found in the original radiosonde file: dry bulb temperature, dewpoint temperature, wind speed, wind direction, eastward wind component, northward wind component, wind status (a Vaisala-produced field used in conjunction with the Loran system), ascent rate, and original relative humidity. Additional humidity fields are smoothed relative humidity, dry biased corrected relative humidity, final ambient relative humidity, and scaled adjusted relative humidity. Third, quality control (QC) flags of the fields from the original radiosonde datastream are brought into the SONDEADJUST output file. Additional QC

  1. Aerosol Optical Depth Value-Added Product for the SAS-He Instrument...

    Office of Scientific and Technical Information (OSTI)

    Aerosol Optical Depth Value-Added Product for the SAS-He Instrument Citation Details In-Document Search Title: Aerosol Optical Depth Value-Added Product for the SAS-He Instrument ...

  2. Top Value Added Chemicals from Biomass: Volume I--Results of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Value Added Chemicals from Biomass: Volume I--Results of Screening for Potential Candidates from Sugars and Synthesis Gas Top Value Added Chemicals from Biomass: Volume I--Results ...

  3. Differential Network Analyses of Alzheimer’s Disease Identify Early Events in Alzheimer’s Disease Pathology

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Xia, Jing; Rocke, David M.; Perry, George; Ray, Monika

    2014-01-01

    In late-onset Alzheimer’s disease (AD), multiple brain regions are not affected simultaneously. Comparing the gene expression of the affected regions to identify the differences in the biological processes perturbed can lead to greater insight into AD pathogenesis and early characteristics. We identified differentially expressed (DE) genes from single cell microarray data of four AD affected brain regions: entorhinal cortex (EC), hippocampus (HIP), posterior cingulate cortex (PCC), and middle temporal gyrus (MTG). We organized the DE genes in the four brain regions into region-specific gene coexpression networks. Differential neighborhood analyses in the coexpression networks were performed to identify genes with lowmore » topological overlap (TO) of their direct neighbors. The low TO genes were used to characterize the biological differences between two regions. Our analyses show that increased oxidative stress, along with alterations in lipid metabolism in neurons, may be some of the very early events occurring in AD pathology. Cellular defense mechanisms try to intervene but fail, finally resulting in AD pathology as the disease progresses. Furthermore, disease annotation of the low TO genes in two independent protein interaction networks has resulted in association between cancer, diabetes, renal diseases, and cardiovascular diseases.« less

  4. Prototype indicators of value added through public involvement

    SciTech Connect (OSTI)

    Lach, D.; Hixson, P.; Ramonas, L.

    1995-12-01

    As more managers realize that public input in public sector decision making is a given in the current political and social climate, many are turning to public involvement as a way to manage input so that it is beneficial to their decisions and projects. Public involvement is starting to become a familiar way of doing business for many Federal agencies and its contractors. Yet, many, if not most agency and contractor managers are still unclear about the value and costs that public involvement adds to their projects. Proponents claim that public involvement increases the acceptability of project goals by increasing stakeholders` knowledge about and involvement in decisions of importance to them. In spite of these assertions avowing the benefits of public involvement, proponents have not generated methods that demonstrate or provide evidence of such value added through incorporating public involvement into projects. As these questions about the value and costs of public involvement efforts increase, there is a pressing need to document the value and costs of public involvement for the participants in these processes--the stakeholders--and to present this information to decision makers in ways that help them assess the value and costs of managing public input through a public involvement program.

  5. No-carrier-added (18F)-N-methylspiroperidol

    DOE Patents [OSTI]

    Shiue, Chyng-Yann; Fowler, Joanna S.; Wolf, Alfred P.

    1993-07-06

    There is disclosed a radioligand labeled with a positron emitting radionuclide suitable for dynamic study in living humans with positron emission transaxial tomography. [.sup.18 F]-N-methylspiroperidol, exhibiting extremely high affinity for the dopamine receptors, provides enhanced uptake and retention in the brain concomitant with reduced radiation burden. These characteristics all combine to provide [.sup.18 F]-N-methylspiroperidol as a radioligand superior to known radioligands for mapping dopamine receptors in normal and disease states in the living brain. Additionally, a new synthetic procedure for this material is disclosed.

  6. No-carrier-added (18F)-N-methylspiroperidol

    DOE Patents [OSTI]

    Shiue, Chyng-Yann; Fowler, Joanna S.; Wolf, Alfred P.

    1993-01-01

    There is disclosed a radioligand labeled with a positron emitting radionuclide suitable for dynamic study in living humans with positron emission transaxial tomography. [.sup.18 F]-N-methylspiroperidol, exhibiting extremely high affinity for the dopamine receptors, provides enhanced uptake and retention in the brain concomitant with reduced radiation burden. These characteristics all combine to provide [.sup.18 F]-N-methylspiroperidol as a radioligand superior to known radioligands for mapping dopamine receptors in normal and disease states in the living brain. Additionally, a new synthetic procedure for this material is disclosed.

  7. Beryllium disease

    SciTech Connect (OSTI)

    Not Available

    1991-12-20

    After two workers at the nuclear weapons plant at Oak Ridge National Laboratory in Tennessee were diagnosed earlier this year with chronic beryllium disease (CBD), a rare and sometimes fatal scarring of the lungs, the Department of Energy ordered up a 4-year probe. Now, part of that probe has begun - tests conducted by the Oak Ridge Associated Universities' Center for Epidemiological Research measuring beryllium sensitivity in 3,000 people who've been exposed to the metal's dust since Manhattan Project managers opened the Y-12 plant at Oak Ridge in 1943. Currently, 119 Y-12 employees process beryllium, which has a number of industrial uses, including rocket heat shields and nuclear weapon and electrical components. The disease often takes 20 to 25 years to develop, and the stricken employees haven't worked with beryllium for years. There is no cure for CBD, estimated to strike 2% of people exposed to the metal. Anti-inflammatory steroids alleviate such symptoms as a dry cough, weight loss, and fatigue. Like other lung-fibrosis diseases that are linked to lung cancer, some people suspect CBD might cause some lung cancer. While difficult to diagnose, about 900 cases of CBD have been reported since a Beryllium Case Registry was established in 1952. The Department of Energy (DOE) estimates that about 10,000 DOE employees and 800,000 people in private industry have worked with beryllium.

  8. Exact solutions with AdS asymptotics of Einstein and Einstein-Maxwell gravity minimally coupled to a scalar field

    SciTech Connect (OSTI)

    Cadoni, Mariano; Serra, Matteo; Mignemi, Salvatore

    2011-10-15

    We propose a general method for solving exactly the static field equations of Einstein and Einstein-Maxwell gravity minimally coupled to a scalar field. Our method starts from an ansatz for the scalar field profile, and determines, together with the metric functions, the corresponding form of the scalar self-interaction potential. Using this method we prove a new no-hair theorem about the existence of hairy black-hole and black-brane solutions and derive broad classes of static solutions with radial symmetry of the theory, which may play an important role in applications of the AdS/CFT correspondence to condensed matter and strongly coupled QFTs. These solutions include: (1) four- or generic (d+2)-dimensional solutions with planar, spherical or hyperbolic horizon topology; (2) solutions with anti-de Sitter, domain wall and Lifshitz asymptotics; (3) solutions interpolating between an anti-de Sitter spacetime in the asymptotic region and a domain wall or conformal Lifshitz spacetime in the near-horizon region.

  9. No-carrier-added [.sup.18 F]-N-fluoroalkylspiroperidols

    DOE Patents [OSTI]

    Shiue, Chyng-Yann; Wolf, Alfred P.; Bai, Lan-Qin; Teng, Ren-Tui

    1989-01-01

    There is disclosed radioligands labeled with the position emitting radionuclide [.sup.18 F] suitable for dynamic study in living humans with position emission transaxial tomography. These new [.sup.18 F]-N-fluoroalkylspiroperidols, wherein the alkyl group contains from 2-6 carbon atoms, exhibit extremely high affinity for the dopamine receptors and provide enhanced uptake and retention in the brain concomitant with reduced radiation burden. These characteristics all combine to make these new radioligands useful for mapping dopamine receptors in normal and disease states in the living brain. Additionally, a new synthetic procedure for these radioligands as well as a new procedure for preparing the radiolabeled alkyl halide alkylating reagents are also disclosed.

  10. Minimal unitary representation of 5d superconformal algebra F(4) and AdS6/CFT5 higher spin (super)-algebras

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Fernando, Sudarshan; Gnaydin, Murat

    2014-11-28

    We study the minimal unitary representation (minrep) of SO(5, 2), obtained by quantization of its geometric quasiconformal action, its deformations and supersymmetric extensions. The minrep of SO(5, 2) describes a massless conformal scalar field in five dimensions and admits a unique deformation which describes a massless conformal spinor. Scalar and spinor minreps of SO(5, 2) are the 5d analogs of Diracs singletons of SO(3, 2). We then construct the minimal unitary representation of the unique 5d supercon-formal algebra F(4) with the even subalgebra SO(5, 2) SU(2). The minrep of F(4) describes a massless conformal supermultiplet consisting of two scalar andmoreone spinor fields. We then extend our results to the construction of higher spin AdS6/CFT5 (super)-algebras. The Joseph ideal of the minrep of SO(5, 2) vanishes identically as operators and hence its enveloping algebra yields the AdS6/CFT5 bosonic higher spin algebra directly. The enveloping algebra of the spinor minrep defines a deformed higher spin algebra for which a deformed Joseph ideal vanishes identically as operators. These results are then extended to the construction of the unique higher spin AdS6/CFT5 superalgebra as the enveloping algebra of the minimal unitary realization of F(4) obtained by the quasiconformal methods.less

  11. MWRRET Value-Added Product: The Retrieval of Liquid Water Path...

    Office of Scientific and Technical Information (OSTI)

    public from the National Technical Information Service, Springfield, VA at www.ntis.gov. ... microwave radiometer (MWR) Retrieval (MWRRET) value-added product (VAP) algorithm. ...

  12. MWRRET Value-Added Product: The Retrieval of Liquid Water Path...

    Office of Scientific and Technical Information (OSTI)

    MWRRET Value-Added Product: The Retrieval of Liquid Water Path and Precipitable Water Vapor from Microwave Radiometer (MWR) Data Sets (Revision 2) Citation Details In-Document ...

  13. Evaluation of the Ad Council/DOE "What's Your Excuse" Public Service

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Advertising Campaign | Department of Energy Ad Council/DOE "What's Your Excuse" Public Service Advertising Campaign Evaluation of the Ad Council/DOE "What's Your Excuse" Public Service Advertising Campaign Evaluation of the Ad Council/DOE "What's Your Excuse" Public Service Advertising Campaign, June 2010, a document prepared for the U.S. Department of Energy's Office of Energy Efficiency and Renewable Energy. Ad Council Campaign Evaluation (1.88 MB) More

  14. Methods for chemical recovery of non-carrier-added radioactive tin from irradiated intermetallic Ti-Sb targets

    DOE Patents [OSTI]

    Lapshina, Elena V.; Zhuikov, Boris L.; Srivastava, Suresh C.; Ermolaev, Stanislav V.; Togaeva, Natalia R.

    2012-01-17

    The invention provides a method of chemical recovery of no-carrier-added radioactive tin (NCA radiotin) from intermetallide TiSb irradiated with accelerated charged particles. An irradiated sample of TiSb can be dissolved in acidic solutions. Antimony can be removed from the solution by extraction with dibutyl ether. Titanium in the form of peroxide can be separated from tin using chromatography on strong anion-exchange resin. In another embodiment NCA radiotin can be separated from iodide solution containing titanium by extraction with benzene, toluene or chloroform. NCA radiotin can be finally purified from the remaining antimony and other impurities using chromatography on silica gel. NCA tin-117m can be obtained from this process. NCA tin-117m can be used for labeling organic compounds and biological objects to be applied in medicine for imaging and therapy of various diseases.

  15. High resolution A/D conversion based on piecewise conversion at lower resolution

    DOE Patents [OSTI]

    Terwilliger, Steve

    2012-06-05

    Piecewise conversion of an analog input signal is performed utilizing a plurality of relatively lower bit resolution A/D conversions. The results of this piecewise conversion are interpreted to achieve a relatively higher bit resolution A/D conversion without sampling frequency penalty.

  16. ADS genes for reducing saturated fatty acid levels in seed oils

    DOE Patents [OSTI]

    Heilmann, Ingo H; Shanklin, John

    2014-03-18

    The present invention relates to enzymes involved in lipid metabolism. In particular, the present invention provides coding sequences for Arabidopsis Desaturases (ADS), the encoded ADS polypeptides, and methods for using the sequences and encoded polypeptides, where such methods include decreasing and increasing saturated fatty acid content in plant seed oils.

  17. ADS genes for reducing saturated fatty acid levels in seed oils

    DOE Patents [OSTI]

    Heilmann, Ingo H.; Shanklin, John

    2010-02-02

    The present invention relates to enzymes involved in lipid metabolism. In particular, the present invention provides coding sequences for Arabidopsis Desaturases (ADS), the encoded ADS polypeptides, and methods for using the sequences and encoded polypeptides, where such methods include decreasing and increasing saturated fatty acid content in plant seed oils.

  18. Scattering Amplitudes, the AdS/CFT Correspondence, Minimal Surfaces, and Integrability

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Alday, Luis F.

    2010-01-01

    We focus on the computation of scattering amplitudes of planar maximally supersymmetric Yang-Mill in four dimensions at strong coupling by means of the AdS/CFT correspondence and explain how the problem boils down to the computation of minimal surfaces in AdS in the first part of this paper. In the second part of this review we explain how integrability allows to give a solution to the problem in terms of a set of integral equations. The intention of the review is to give a pedagogical, rather than very detailed, exposition.

  19. CYBERSECURITY AND USER ACCOUNTABILITY IN THE C-AD CONTROL SYSTEM

    SciTech Connect (OSTI)

    MORRIS,J.T.; BINELLO, S.; D OTTAVIO, T.; KATZ, R.A.

    2007-10-15

    A heightened awareness of cybersecurity has led to a review of the procedures that ensure user accountability for actions performed on the computers of the Collider-Accelerator Department (C-AD) Control System. Control system consoles are shared by multiple users in control rooms throughout the C-AD complex. A significant challenge has been the establishment of procedures that securely control and monitor access to these shared consoles without impeding accelerator operations. This paper provides an overview of C-AD cybersecurity strategies with an emphasis on recent enhancements in user authentication and tracking methods.

  20. EIA Energy Efficiency-Table 3d. Value Added by Selected Industries...

    Gasoline and Diesel Fuel Update (EIA)

    d Page Last Modified: May 2010 Table 3d. Value Added1 by Selected Industries, 1998, 2002, and 2006 (Current Brillion Dollars) MECS Survey Years NAICS Subsector and Industry 1998...

  1. EIA Energy Efficiency-Table 4d. Value Added by Selected Industries...

    Gasoline and Diesel Fuel Update (EIA)

    d Page Last Modified: May 2010 Table 4d. Value Added1 by Selected Industries, 1998, 2002, and 2006 (Billion 2000 Dollars 2) MECS Survey Years NAICS Subsector and Industry 1998 2002...

  2. Toward the AdS/CFT gravity dual for high energy collisions. II...

    Office of Scientific and Technical Information (OSTI)

    II. The stress tensor on the boundary Citation Details In-Document Search Title: Toward the AdSCFT gravity dual for high energy collisions. II. The stress tensor on the boundary ...

  3. DOE/SC-ARM/TR-100 Raman Lidar Profiles Best Estimate Value-Added...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    0 Raman Lidar Profiles Best Estimate Value-Added Product Technical Report R Newsom January 2012 DISCLAIMER This report was prepared as an account of work sponsored by the U.S. ...

  4. Top Value Added Chemicals from Biomass: Volume I--Results of Screening for

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Potential Candidates from Sugars and Synthesis Gas | Department of Energy Value Added Chemicals from Biomass: Volume I--Results of Screening for Potential Candidates from Sugars and Synthesis Gas Top Value Added Chemicals from Biomass: Volume I--Results of Screening for Potential Candidates from Sugars and Synthesis Gas This report identifies twelve building block chemicals that can be produced from sugars via biological or chemical conversions. 35523.pdf (1.41 MB) More Documents &

  5. Top Value-Added Chemicals from Biomass - Volume II„Results of Screening

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    for Potential Candidates from Biorefinery Lignin | Department of Energy Value-Added Chemicals from Biomass - Volume II„Results of Screening for Potential Candidates from Biorefinery Lignin Top Value-Added Chemicals from Biomass - Volume II„Results of Screening for Potential Candidates from Biorefinery Lignin This report evaluates lignins role as a renewable raw material resource. pnnl-16983.pdf (1006.18 KB) More Documents & Publications Low Cost Carbon Fiber from Renewable Resources

  6. AdS/QCD and Applications of Light-Front Holography

    SciTech Connect (OSTI)

    Brodsky, Stanley J.; Cao, Fu-Guang; de Teramond, Guy F.; /Costa Rica U.

    2012-02-16

    Light-Front Holography leads to a rigorous connection between hadronic amplitudes in a higher dimensional anti-de Sitter (AdS) space and frame-independent light-front wavefunctions of hadrons in 3 + 1 physical space-time, thus providing a compelling physical interpretation of the AdS/CFT correspondence principle and AdS/QCD, a useful framework which describes the correspondence between theories in a modified AdS5 background and confining field theories in physical space-time. To a first semiclassical approximation, where quantum loops and quark masses are not included, this approach leads to a single-variable light-front Schroedinger equation which determines the eigenspectrum and the light-front wavefunctions of hadrons for general spin and orbital angular momentum. The coordinate z in AdS space is uniquely identified with a Lorentz-invariant coordinate {zeta} which measures the separation of the constituents within a hadron at equal light-front time. The internal structure of hadrons is explicitly introduced and the angular momentum of the constituents plays a key role. We give an overview of the light-front holographic approach to strongly coupled QCD. In particular, we study the photon-to-meson transition form factors (TFFs) F{sub M{gamma}}(Q{sup 2}) for {gamma}{gamma}* {yields} M using light-front holographic methods. The results for the TFFs for the {eta} and {eta}' mesons are also presented. Some novel features of QCD are discussed, including the consequences of confinement for quark and gluon condensates. A method for computing the hadronization of quark and gluon jets at the amplitude level is outlined.

  7. Expert Meeting Report: Key Innovations for Adding Energy Efficiency to Maintenance Projects

    SciTech Connect (OSTI)

    Wood, A.; Wiehagen, J.

    2012-09-01

    This report describes an expert meeting hosted by the Building America research team NAHB Research Center, which was held on February 8, 2012, in Orlando, Florida. The topic, Key Innovations for Adding Energy Efficiency to Maintenance Projects, was intended to provide direction to more focused efforts to increase the efficiency of existing homes; in this meeting, the focus was specifically for re-roofing and the opportunities for adding energy efficiency upgrades during this major home repair activity.

  8. Building America Expert Meeting: Key Innovations for Adding Energy Efficiency to Maintenance Projects

    Office of Energy Efficiency and Renewable Energy (EERE)

    This report describes an expert meeting hosted by the Building America research team NAHB Research Center, which was held on February 8, 2012, in Orlando, Florida. The topic, Key Innovations for Adding Energy Efficiency to Maintenance Projects, was intended to provide direction to more focused efforts to increase the efficiency of existing homes; in this meeting, the focus was specifically for re-roofing and the opportunities for adding energy efficiency upgrades during this major home repair activity.

  9. DOE Announces Webinars on Adding Solar to Your Building Portfolio, How to

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Use DOE's Newest Facilities, and More | Department of Energy Adding Solar to Your Building Portfolio, How to Use DOE's Newest Facilities, and More DOE Announces Webinars on Adding Solar to Your Building Portfolio, How to Use DOE's Newest Facilities, and More February 27, 2014 - 4:48pm Addthis EERE offers webinars to the public on a range of subjects, from adopting the latest energy efficiency and renewable energy technologies to training for the clean energy workforce. Webinars are free;

  10. Role of paraoxonase 1 (PON1) in organophosphate metabolism: Implications in neurodegenerative diseases

    SciTech Connect (OSTI)

    Androutsopoulos, Vasilis P.; Kanavouras, Konstantinos; Tsatsakis, Aristidis M.

    2011-11-15

    Organophosphate pesticides are a class of compounds that are widely used in agricultural and rural areas. Paraoxonase 1 (PON1) is a phase-I enzyme that is involved in the hydrolysis of organophosphate esters. Environmental poisoning by organophosphate compounds has been the main driving force of previous research on PON1 enzymes. Recent discoveries in animal models have revealed the important role of the enzyme in lipid metabolism. However although PON1 function is well established in experimental models, the contribution of PON1 in neurodegenerative diseases remains unclear. In this minireview we summarize the involvement of PON1 genotypes in the occurrence of Parkinson's disease, Alzheimer's disease and amyotrophic lateral sclerosis. A brief overview of latest epidemiological studies, regarding the two most important PON1 coding region polymorphisms PON1-L55M and PON1-Q192R is presented. Positive and negative associations of PON1 with disease occurrence are reported. Notably the MM and RR alleles contribute a risk enhancing effect for the development of some neurodegenerative diseases, which may be explained by the reduced lipoprotein free radical scavenging activity that may give rise to neuronal damage, through distinct mechanism. Conflicting findings that fail to support this postulate may represent the human population ethnic heterogeneity, different sample size and environmental parameters affecting PON1 status. We conclude that further epidemiological studies are required in order to address the exact contribution of PON1 genome in combination with organophosphate exposure in populations with neurodegenerative diseases.

  11. Nonasymptotically AdS/dS solutions and their higher dimensional origins

    SciTech Connect (OSTI)

    Cai Ronggen; Wang Anzhong

    2004-10-15

    We look for and analyze in some details some exact solutions of Einstein-Maxwell-dilaton gravity with one or two Liouville-type dilaton potential(s) in an arbitrary dimension. Such a theory could be obtained by dimensionally reducing Einstein-Maxwell theory with a cosmological constant to a lower dimension. These (neutral/magnetic/electric charged) solutions can have a (two) black hole horizon(s), cosmological horizon, or a naked singularity. Black hole horizon or cosmological horizon of these solutions can be a hypersurface of positive, zero or negative constant curvature. These exact solutions are neither asymptotically flat, nor asymptotically AdS/dS. But some of them can be uplifted to a higher dimension, and those higher dimensional solutions are either asymptotically flat, or asymptotically AdS/dS with/without a compact constant curvature space. This observation is useful to better understand holographic properties of these nonasymptotically AdS/dS solutions.

  12. Adding Links in Energy.gov Content Management System | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy.gov Content Management System » Adding Links in Energy.gov Content Management System Adding Links in Energy.gov Content Management System For Office of Energy Efficiency and Renewable Energy (EERE) websites, to add a link in the Energy.gov Drupal content management system (CMS), follow these steps: Edit the page (also called a node) that you want to add a link to. Select the text you want to link. Click the "Link" button. Then: If you are linking to a page on Energy.gov, select

  13. Adding "Utilization" to Carbon Capture and Storage | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Adding "Utilization" to Carbon Capture and Storage Adding "Utilization" to Carbon Capture and Storage May 1, 2012 - 4:23pm Addthis Greater application of CO2-EOR could yield a significant boost to the U.S. economy, including increased economic activity, improved balance of trade, job creation, and reduced oil imports. Greater application of CO2-EOR could yield a significant boost to the U.S. economy, including increased economic activity, improved balance of trade,

  14. Process and apparatus for adding and removing particles from pressurized reactors

    DOE Patents [OSTI]

    Milligan, John D.

    1983-01-01

    A method for adding and removing fine particles from a pressurized reactor is provided, which comprises connecting the reactor to a container, sealing the container from the reactor, filling the container with particles and a liquid material compatible with the reactants, pressurizing the container to substantially the reactor pressure, removing the seal between the reactor and the container, permitting particles to fall into or out of the reactor, and resealing the container from the reactor. An apparatus for adding and removing particles is also disclosed.

  15. I INSTRUCTIONS: FROM: Accountability Station Energy Library, AD-622.1/GTN

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    I INSTRUCTIONS: FROM: Accountability Station Energy Library, AD-622.1/GTN U.S. Department of Energy P.O. Box A Germantown, MD 20874 Document Number Copy Series Classi- fication Date Due TO: I have personally received the material identified above and assume full responsibility for its safe handling in accordance with existing regulations. Date Signature Postal Registry No. AD F 1430.1 (11-92) U.S. DEPARTMENT OF ENERGY WASHINGTON, D.C. ENERGY LIBRARY CLASSIFIED RECEIPT NO. T 1. Original of this

  16. Interim Chronic Beryllium Disease

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1999-12-08

    Establishes Departmental expectations for addressing chronic beryllium disease throughout the Department until a Departmental rule on beryllium is promulgated. This Notice was replaced by final rule 10 CFR Part 850, Chronic Beryllium Disease Prevention Program, published December 8, 1999.

  17. G-Band Vapor Radiometer Precipitable Water Vapor (GVRPWV) Value-Added Product

    SciTech Connect (OSTI)

    Koontz, A; Cadeddu, M

    2012-12-05

    The G-Band Vapor Radiometer Precipitable Water Vapor (GVRPWV) value-added product (VAP) computes precipitable water vapor using neural network techniques from data measured by the GVR. The GVR reports time-series measurements of brightness temperatures for four channels located at 183.3 ± 1, 3, 7, and 14 GHz.

  18. Superstrings on AdS{sub 4}xCP{sup 3} from supergravity

    SciTech Connect (OSTI)

    D'Auria, Riccardo; Trigiante, Mario; Fre, Pietro; Grassi, Pietro Antonio

    2009-04-15

    We derive from a general formulation of pure spinor string theory on type IIA backgrounds the specific form of the action for the AdS{sub 4}xCP{sup 3} background. We provide a complete geometrical characterization of the structure of the superfields involved in the action.

  19. Expert Meeting Report. Key Innovations for Adding Energy Efficiency to Maintenance Projects

    SciTech Connect (OSTI)

    Wood, A.; Wiehagen, J.

    2012-09-01

    This report describes an expert meeting hosted by the Building America research team NAHB Research Center, which was held on February 8, 2012, in Orlando, Florida. The topic focused on efforts to increase the efficiency of existing homes, specifically for re-roofing and the opportunities for adding energy efficiency upgrades during this major home repair activity.

  20. Evaluation of the Ad Council/DOE Whats Your ExcuseŽ Public...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Across all four outcomes, one or both of the control variables (predilection to save energy and perceived difficulty of saving energy) were statistically significant. Ad ...

  1. PNNI routing support for ad hoc mobile networking: A flat architecture

    SciTech Connect (OSTI)

    Martinez, L.; Sholander, P.; Tolendino, L.

    1997-12-01

    This contribution extends the Outside Nodal Hierarchy List (ONHL) procedures described in ATM Form Contribution 97-0766. These extensions allow multiple mobile networks to form either an ad hoc network or an extension of a fixed PNNI infrastructure. This contribution covers the simplest case where the top-most Logical Group Nodes (LGNs), in those mobile networks, all reside at the same level in a PNNI hierarchy. Future contributions will cover the general case where those top-most LGNs reside at different hierarchy levels. This contribution considers a flat ad hoc network architecture--in the sense that each mobile network always participates in the PNNI hierarchy at the preconfigured level of its top-most LGN.

  2. First measurement of ADS parameters using $B^- \\to D^0K^-$ decays in hadron collisions

    SciTech Connect (OSTI)

    Garosi, Paola; /Siena U. /INFN, Pisa

    2011-06-01

    Measurements of branching fractions and CP-asymmetries of B{sup -} {yields} D{sup 0}K{sup -} modes allow a theoretically-clean extraction of the CKM angle {gamma}. The method proposed by Atwood, Dunietz and Soni (ADS) makes use of a decay chain where color and Cabibbo suppression interfere, which produces large CP-violating asymmetries. The CDF experiment reports the first measurement at a hadron collider of branching fractions and CP-asymmetries of suppressed B{sup -} {yields} D{sup 0}h{sup -} signals, where h is {pi} or K. Using 5.0 fb{sup -1} of data we found a combined significance exceeding 5{sigma} and we determined the ADS parameters with accuracy comparable with B-factories.

  3. Dilatonic repulsons and confinement via the AdS/CFT correspondence

    SciTech Connect (OSTI)

    Bak, Dongsu; Gutperle, Michael; Hirano, Shinji; Ohta, Nobuyoshi

    2004-10-15

    We study a class of dilatonic deformations of asymptotically AdS{sub 5}xS{sup 5} geometry analytically and numerically. The spacetime is nonsupersymmetric and suffers from a naked singularity. We propose that the causality bound may serve as a criterion for such a geometry with a naked singularity to still make sense in the AdS/CFT correspondence. We show that the static string, the one corresponding to a large Wilson loop in the dual gauge theory, reveals confinement in a certain range of parameters of our solutions, where the singularity exhibits the repulsion that can well cloak the singularity from the static string probe. In particular, we find the exact expression for the tension of the QCD strings. We also discuss a possible interpretation of our solution in terms of unstable branes and their tachyon matter.

  4. Added Value of Reliability to a Microgrid: Simulations of Three California Buildings

    SciTech Connect (OSTI)

    Marnay, Chris; Lai, Judy; Stadler, Michael; Siddiqui, Afzal

    2009-05-07

    The Distributed Energy Resources Customer Adoption Model is used to estimate the value an Oakland nursing home, a Riverside high school, and a Sunnyvale data center would need to put on higher electricity service reliability for them to adopt a Consortium for Electric Reliability Technology Solutions Microgrid (CM) based on economics alone. A fraction of each building's load is deemed critical based on its mission, and the added cost of CM capability to meet it added to on-site generation options. The three sites are analyzed with various resources available as microgrid components. Results show that the value placed on higher reliability often does not have to be significant for CM to appear attractive, about 25 $/kWcdota and up, but the carbon footprint consequences are mixed because storage is often used to shift cheaper off-peak electricity to use during afternoon hours in competition with the solar sources.

  5. Exact quantization of a superparticle in AdS{sub 5}xS{sup 5}

    SciTech Connect (OSTI)

    Horigane, Tetsuo; Kazama, Yoichi

    2010-02-15

    As a step toward deeper understanding of the AdS/CFT correspondence, exact quantization of a Brink-Schwarz superparticle in the AdS{sub 5}xS{sup 5} background with Ramond-Ramond flux is performed from the first principle in the phase space formulation. It includes the construction of the quantum Noether charges for the psu(2,2|4) superconformal symmetry and by solving the superconformal primary conditions we obtain the complete physical spectrum of the system with the explicit wave functions. The spectrum agrees precisely with the supergravity results, including all the Kaluza-Klein excitations. Our method and the result are expected to shed light on the eventual quantization of a superstring in this important background.

  6. Methods for conversion of carbohydrates in ionic liquids to value-added chemicals

    DOE Patents [OSTI]

    Zhao, Haibo; Holladay, Johnathan E.

    2011-05-10

    Methods are described for converting carbohydrates including, e.g., monosaccharides, disaccharides, and polysaccharides in ionic liquids to value-added chemicals including furans, useful as chemical intermediates and/or feedstocks. Fructose is converted to 5-hydroxylmethylfurfural (HMF) in the presence of metal halide and acid catalysts. Glucose is effectively converted to HMF in the presence of chromium chloride catalysts. Yields of up to about 70% are achieved with low levels of impurities such as levulinic acid.

  7. Quality improvement of pyrolysis oil from waste rubber by adding sawdust

    SciTech Connect (OSTI)

    Wang, Wen-liang; Chang, Jian-min; Cai, Li-ping; Shi, Sheldon Q.

    2014-12-15

    Highlights: • Rubber-pyrolysis oil is difficult to be fuel due to high proportion of PAHs. • The efficiency of pyrolysis was increased as the percentage of sawdust increased. • The adding of sawdust improved pyrolysis oil quality by reducing the PAHs content. • Adding sawdust reduced nitrogen/sulfur in oil and was easier to convert to diesel. - Abstract: This work was aimed at improving the pyrolysis oil quality of waste rubber by adding larch sawdust. Using a 1 kg/h stainless pyrolysis reactor, the contents of sawdust in rubber were gradually increased from 0%, 50%, 100% and 200% (wt%) during the pyrolysis process. Using a thermo-gravimetric (TG) analyzer coupled with Fourier transform infrared (FTIR) analysis of evolving products (TG–FTIR), the weight loss characteristics of the heat under different mixtures of sawdust/rubber were observed. Using the pyrolysis–gas chromatography (GC)–mass spectrometry (Py–GC/MS), the vapors from the pyrolysis processes were collected and the compositions of the vapors were examined. During the pyrolysis process, the recovery of the pyrolysis gas and its composition were measured in-situ at a reaction temperature of 450 °C and a retaining time of 1.2 s. The results indicated that the efficiency of pyrolysis was increased and the residual carbon was reduced as the percentage of sawdust increased. The adding of sawdust significantly improved the pyrolysis oil quality by reducing the polycyclic aromatic hydrocarbons (PAHs) and nitrogen and sulfur compounds contents, resulting in an improvement in the combustion efficiency of the pyrolysis oil.

  8. ARM Climate Research Facility Quarterly Value-Added Product Report, Fourth Quarter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    72 ARM Climate Research Facility Quarterly Value-Added Product Report C Sivaraman October 2015 DISCLAIMER This report was prepared as an account of work sponsored by the U.S. Government. Neither the United States nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not

  9. DOE/SC-ARM-TR-140 Droplet Number Concentration Value-Added Product

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    0 Droplet Number Concentration Value-Added Product L Riihimaki S McFarlane C Sivaraman June 2014 Version 1.0 DISCLAIMER This report was prepared as an account of work sponsored by the U.S. Government. Neither the United States nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its

  10. DOE/SC-ARM/TR-087 Merged Sounding Value-Added Product D Troyan

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    87 Merged Sounding Value-Added Product D Troyan March 2012 DISCLAIMER This report was prepared as an account of work sponsored by the U.S. Government. Neither the United States nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights.

  11. DOE/SC-ARM/TR-098 Micropulse Lidar Cloud Mask Value-Added Product Technical Report

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    8 Micropulse Lidar Cloud Mask Value-Added Product Technical Report C Sivaraman J Comstock July 2011 DISCLAIMER This report was prepared as an account of work sponsored by the U.S. Government. Neither the United States nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would

  12. DOE/SC-ARM/TR-115 Aerosol Best Estimate (AEROSOLBE) Value-Added Product

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    5 Aerosol Best Estimate (AEROSOLBE) Value-Added Product C Flynn D Turner A Koontz D Chand C Sivaraman July 2012 DISCLAIMER This report was prepared as an account of work sponsored by the U.S. Government. Neither the United States nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that

  13. DOE/SC-ARM/TR-129 Aerosol Optical Depth Value-Added Product

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    9 Aerosol Optical Depth Value-Added Product A Koontz C Flynn G Hodges J Michalsky J Barnard March 2013 DISCLAIMER This report was prepared as an account of work sponsored by the U.S. Government. Neither the United States nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use

  14. Microsoft Word - ARM Value-Added Product_tech_rpt_v2.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    77 An Atmospheric Radiation Measurement Value-Added Product to Retrieve Optically Thin Cloud Visible Optical Depth using Micropulse Lidar October 2006 Chaomei Lo Jennifer M. Comstock Connor Flynn Pacific Northwest National Laboratory Richland, Washington Work supported by the U.S. Department of Energy, Office of Energy Research, Office of Biological and Environmental Research C Lo, JM Comstock, C Flynn, October 2006, ARM TR-077 iii Contents 1 Introduction

  15. Catalytic Conversion of Biomass-derived Feedstock (HMF) into Value Added

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Chemicals and Biofuels - Energy Innovation Portal Industrial Technologies Industrial Technologies Biomass and Biofuels Biomass and Biofuels Find More Like This Return to Search Catalytic Conversion of Biomass-derived Feedstock (HMF) into Value Added Chemicals and Biofuels Colorado State University Contact CSU About This Technology Technology Marketing Summary A catalytic reaction system by which the biomass-derived feedstock chemical HMF can be upgraded into a higher carbon content

  16. Recent Developments on the Broadband Heating Rate Profile Value-Added Product

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Recent Developments on the Broadband Heating Rate Profile Value-Added Product E. J. Mlawer, J. S. Delamere, and S. A. Clough Atmospheric and Environmental Research, Inc. Cambridge, Massachusetts M. A. Miller and K. L. Johnson Brookhaven National Laboratory Upton, New York T. R. Shippert and C. N. Long Pacific Northwest National Laboratory Richland, Washington R. G. Ellingson Florida State University Tallahassee, Florida M. H. Zhang State University of New York - Stony Brook Albany, New York R.

  17. Method for conversion of carbohydrate polymers to value-added chemical products

    DOE Patents [OSTI]

    Zhang, Zongchao C.; Brown, Heather M.; Su, Yu

    2012-02-07

    Methods are described for conversion of carbohydrate polymers in ionic liquids, including cellulose, that yield value-added chemicals including, e.g., glucose and 5-hydroxylmethylfurfural (HMF) at temperatures below 120.degree. C. Catalyst compositions that include various mixed metal halides are described that are selective for specified products with yields, e.g., of up to about 56% in a single step process.

  18. Flying across Galaxy Clusters with Google Earth: additional imagery from SDSS co-added data

    SciTech Connect (OSTI)

    Hao, Jiangang; Annis, James; /Fermilab

    2010-10-01

    Galaxy clusters are spectacular. We provide a Google Earth compatible imagery for the deep co-added images from the Sloan Digital Sky Survey and make it a tool for examing galaxy clusters. Google Earth (in sky mode) provides a highly interactive environment for visualizing the sky. By encoding the galaxy cluster information into a kml/kmz file, one can use Google Earth as a tool for examining galaxy clusters and fly across them freely. However, the resolution of the images provided by Google Earth is not very high. This is partially because the major imagery google earth used is from Sloan Digital Sky Survey (SDSS) (SDSS collaboration 2000) and the resolutions have been reduced to speed up the web transferring. To have higher resolution images, you need to add your own images in a way that Google Earth can understand. The SDSS co-added data are the co-addition of {approx}100 scans of images from SDSS stripe 82 (Annis et al. 2010). It provides the deepest images based on SDSS and reach as deep as about redshift 1.0. Based on the co-added images, we created color images in a way as described by Lupton et al. (2004) and convert the color images to Google Earth compatible images using wcs2kml (Brewer et al. 2007). The images are stored at a public server at Fermi National Accelerator Laboratory and can be accessed by the public. To view those images in Google Earth, you need to download a kmz file, which contains the links to the color images, and then open the kmz file with your Google Earth. To meet different needs for resolutions, we provide three kmz files corresponding to low, medium and high resolution images. We recommend the high resolution one as long as you have a broadband Internet connection, though you should choose to download any of them, depending on your own needs and Internet speed. After you open the downloaded kmz file with Google Earth (in sky mode), it takes about 5 minutes (depending on your Internet connection and the resolution of images you

  19. Adventures in Infectious Diseases

    ScienceCinema (OSTI)

    Fisher-Hoch, Susan [University of Texas School of Public Health

    2014-06-25

    Dr. Susan Fisher-Hoch, Virologist and Epidemiologist, will discuss her research and travels associated with viral hemorrhagic fevers. From the Ebola outbreak in Reston, Virginia to outbreaks of Crimean Congo Hemorrhagic Fever in South Africa, Senegal, and Saudi Arabia, Dr. Fisher-Hoch has studied and tracked the pathophysiology of these viral diseases. These studies have led her from the Center for Disease Control in the United States, to Lyon, France where she was instrumental in designing, constructing, and rendering operational a laboratory capable of containing some of the world's most dangerous diseases.

  20. DOE/SC-ARM/TR-133 Aerosol Optical Depth Value-Added

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    3 Aerosol Optical Depth Value-Added Product for the SAS-He Instrument B Ermold CJ Flynn J Barnard September 2013 Version 1.0 DISCLAIMER This report was prepared as an account of work sponsored by the U.S. Government. Neither the United States nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or

  1. System for adding sulfur to a fuel cell stack system for improved fuel cell stability

    DOE Patents [OSTI]

    Mukerjee, Subhasish; Haltiner, Jr., Karl J; Weissman, Jeffrey G

    2013-08-13

    A system for adding sulfur to a reformate stream feeding a fuel cell stack, having a sulfur source for providing sulfur to the reformate stream and a metering device in fluid connection with the sulfur source and the reformate stream. The metering device injects sulfur from the sulfur source to the reformate stream at a predetermined rate, thereby providing a conditioned reformate stream to the fuel cell stack. The system provides a conditioned reformate stream having a predetermined sulfur concentration that gives an acceptable balance of minimal drop in initial power with the desired maximum stability of operation over prolonged periods for the fuel cell stack.

  2. Reconstruction of spatial patterns of climatic anomalies during the medieval warm period (AD 900-1300)

    SciTech Connect (OSTI)

    Diaz, H.F.; Hughes, M.K.

    1992-12-31

    The workshop will focus on climatic variations during the Medieval Warm Period or Little Climatic Optimum. The nominal time interval assigned to this period is AD 900--1300, but climate information available during the century or two preceding and following this episode is welcome. The aims of the workshop will be to: examine the available evidence for the existence of this episode; assess the spatial and temporal synchronicity of the climatic signals; discuss possible forcing mechanisms; and identify areas and paleoenvironmental records where additional research efforts are needed to improve our knowledge of this period. This document consists of abstracts of eighteen papers presented at the meeting.

  3. Functionally gradient material for membrane reactors to convert methane gas into value-added products

    DOE Patents [OSTI]

    Balachandran, U.; Dusek, J.T.; Kleefisch, M.S.; Kobylinski, T.P.

    1996-11-12

    A functionally gradient material for a membrane reactor for converting methane gas into value-added-products includes an outer tube of perovskite, which contacts air; an inner tube which contacts methane gas, of zirconium oxide, and a bonding layer between the perovskite and zirconium oxide layers. The bonding layer has one or more layers of a mixture of perovskite and zirconium oxide, with the layers transitioning from an excess of perovskite to an excess of zirconium oxide. The transition layers match thermal expansion coefficients and other physical properties between the two different materials. 7 figs.

  4. Functionally gradient material for membrane reactors to convert methane gas into value-added products

    DOE Patents [OSTI]

    Balachandran, Uthamalingam; Dusek, Joseph T.; Kleefisch, Mark S.; Kobylinski, Thadeus P.

    1996-01-01

    A functionally gradient material for a membrane reactor for converting methane gas into value-added-products includes an outer tube of perovskite, which contacts air; an inner tube which contacts methane gas, of zirconium oxide, and a bonding layer between the perovskite and zirconium oxide layers. The bonding layer has one or more layers of a mixture of perovskite and zirconium oxide, with the layers transitioning from an excess of perovskite to an excess of zirconium oxide. The transition layers match thermal expansion coefficients and other physical properties between the two different materials.

  5. Atmospheric Radiation Measurement (ARM) Value-Added Data Products (Including Evaluated Data Sets)

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Many of the scientific needs of the ARM Program are met through the analysis and processing of existing data products into "value-added" products or VAPs. Despite extensive instrumentation deployed at the ARM sites, there will always be quantities of interest that are either impractical or impossible to measure directly or routinely. Physical models using ARM instrument data as inputs are implemented as VAPs and can help fill some of the unmet measurement needs of the Program. Conversely, ARM produces some VAPs not in order to fill unmet measurement needs, but instead to improve the quality of existing measurements. In addition, when more than one measurement is available, ARM also produces "best estimate" VAPs. A special class of VAP called a Quality Measurement Experiment (QME) adds value to the input data streams by providing for continuous assessment of the quality of the input data. [taken from http://www.arm.gov/data/vaps_all.php] One of the ARM data centers, the External Data Center or XDC at Brookhaven National Laboratory, also adds value to ARM information by identifying sources and acquiring external data to augment the data being generated within the program. These external data sets are converted, processed, and carefully evaluated for their value to the overall ARM program. /. Data Plots are also value-added products from ARM.

  6. Combustion process and nitrogen oxides emission of Shenmu coal added with sodium acetate

    SciTech Connect (OSTI)

    Yang Weijuan; Zhou Junhu; Liu Maosheng; Zhou Zhijun; Liu Jianzhong; Cen Kefa

    2007-09-15

    Shenmu bituminous coal with 4% sodium acetate added was used to investigate the characteristics of combustion and nitrogen oxide (NOx) release in a fixed bed reactor heated by a tube furnace. The composition of the flue gas was analyzed to investigate the effects of sodium acetate on the combustion process and NOx emission. The experiments were carried out in a partial reductive atmosphere and a strong oxidative atmosphere. The O{sub 2} valley value in the partial reductive atmosphere was reduced by the added sodium acetate. Sodium acetate accelerated the combustion and shortened the combustion process. The experimental results showed that the emissions of NO, NO{sub 2}, and N{sub 2}O were affected by the reacting atmosphere and the combustion temperature. In the strong oxidative atmosphere, sodium acetate resulted in a slight NOx reduction. In the partial reductive atmosphere, sodium acetate reduced both the peak value of NO concentration and the total NO emission significantly. An over 30% NOx reduction efficiency was achieved at 900{sup o}C in the partial reductive atmosphere, which decreased with the increase in temperature. Sodium acetate was decomposed into hydrocarbon radicals and sodium hydroxide, which can both reduce NOx emissions due to their special reactions with the nitrogen component. 17 refs., 11 figs., 2 tabs.

  7. Value Added Products from Hemicellulose Utilization in Dry Mill Ethanol Plants

    SciTech Connect (OSTI)

    Rodney Williamson, ICPB; John Magnuson, PNNL; David Reed, INL; Marco Baez, Dyadic; Marion Bradford, ICPB

    2007-03-30

    The Iowa Corn Promotion Board is the principal contracting entity for this grant funded by the US Department of Agriculture and managed by the US Department of Energy. The Iowa Corn Promotion Board subcontracted with New Jersey Institute of Technology, KiwiChem, Pacific Northwest National Lab and Idaho National Lab to conduct research for this project. KiwiChem conducted the economic engineering assessment of a dry-mill ethanol plant. New Jersey Institute of Technology conducted work on incorporating the organic acids into polymers. Pacific Northwest National Lab conducted work in hydrolysis of hemicellulose, fermentation and chemical catalysis of sugars to value-added chemicals. Idaho National Lab engineered an organism to ferment a specific organic acid. Dyadic, an enzme company, was a collaborator which provided in-kind support for the project. The Iowa Corn Promotion Board collaborated with the Ohio Corn Marketing Board and the Minnesota Corn Merchandising Council in providing cost share for the project. The purpose of this diverse collaboration was to integrate the hydrolysis, the conversion and the polymer applications into one project and increase the likelihood of success. This project had two primary goals: (1) to hydrolyze the hemicellulose fraction of the distillers grain (DG) coproduct coming from the dry-mill ethanol plants and (2) convert the sugars derived from the hemicellulose into value-added co-products via fermentation and chemical catalysis.

  8. Flat space S matrix from the AdS/CFT correspondence?

    SciTech Connect (OSTI)

    Gary, Michael; Giddings, Steven B.

    2009-08-15

    We investigate recovery of the bulk S matrix from the AdS/CFT correspondence, at large radius. It was recently argued that some of the elements of the S matrix might be read from CFT correlators, given a particular singularity structure of the latter, but leaving the question of more general S matrix elements. Since in AdS/CFT, data must be specified on the boundary, we find certain limitations on the corresponding bulk wave packets and on their localization properties. In particular, those we have found that approximately localize have low-energy tails, and corresponding power-law tails in position space. When their scattering is compared to that of 'sharper' wave packets typically used in scattering theory, one finds apparently significant differences, suggesting a possible lack of resolution via these wave packets. We also give arguments that construction of the sharper wave packets may require nonperturbative control of the boundary theory, and particularly of the N{sup 2} matrix degrees of freedom. These observations thus raise interesting questions about what principle would guarantee the appropriate control, and about how a boundary CFT can accurately approximate the flat space S matrix.

  9. Value-Added Products from FGD Sulfite-Rich Scrubber Materials

    SciTech Connect (OSTI)

    Vivak Malhotra

    2010-01-31

    According to the American Coal Ash Association, about 29.25 million tons of flue gas desulfurization (FGD) byproducts were produced in the USA in 2003. Out of 29.25 million tons, 17.35 million tons were sulfite-rich scrubber materials. At present, unlike its cousin FGD gypsum, the prospect for effective utilization of sulfite-rich scrubber materials is not bright. In fact, almost 16.9 million tons are leftover every year. In our pursuit to mitigate the liability of sulfite-rich FGD scrubber materials' disposal, we are attempting to develop value-added products that can commercially compete. More specifically, for this Innovative Concept Phase I project, we have the following objectives: to characterize the sulfite-rich scrubber material for toxic metals; to optimize the co-blending and processing of scrubber material and natural byproducts; to formulate and develop structural composites from sulfite-rich scrubber material; and to evaluate the composites' mechanical properties and compare them with current products on the market. After successfully demonstrating the viability of our research, a more comprehensive approach will be proposed to take these value-added materials to fruition.

  10. EA-0896: Finding of No Significant Impact

    Broader source: Energy.gov [DOE]

    Center for Nuclear Medicine Research in Alzheimer's Disease Health Sciences Center- West Virginia University

  11. EA-0896: Final Environmental Assessment

    Broader source: Energy.gov [DOE]

    Center for Nuclear Medicine Research in Alzheimer's Disease Health Sciences Center- West Virginia University

  12. Energy-efficient distributed constructions of miniumum spanning tree for wireless ad-hoc networks

    SciTech Connect (OSTI)

    Kumar, V. S. A.; Pandurangan, G.; Khan, M.

    2004-01-01

    The Minimum Spanning Tree (MST) problem is one of the most important and commonly occurring primitive in the design and operation of data and communication networks. While there a redistributed algorithms for the MST problem these require relatively large number of messages and time, and are fairly involved, require synchronization and a lot of book keeping; this makes these algorithms impractical for emerging technologies such as ad hoc and sensor networks. In such networks, a sensor has very limited power, and any algorithm needs to be simple, local and energy efficient for being practical. Motivated by these considerations, we study the performance of a class of simple and local algorithms called Nearest Neighbor Tree (NNT) algorithms for energy-efficient construction of MSTs in a wireless ad hoc setting. These employ a very simple idea to eliminate the work involved in cycle detection in other MST algorithms: each node chooses a distinct rank, and connects to the closest node of higher rank. We consider two variants of the NNT algorithms, obtained by two ways of choosing the ranks: (i) Random NNT, in which each node chooses a rank randomly, and (ii) Directional NNT, in which each node uses directional information for choosing the rank. We show provable bounds on the performance of these algorithms in instances obtained by uniformly distributed points in the unit square. Finally, we perform extensive simulations of our algorithms. We tested our algorithms on both uniformly random distributions of points, and on realistic distributions of points in an urban setting. The cost of the tree found by the NNT algorithms is within a factor of 2 of the MST, but there is more than a ten-fold saving on the energy and about a five fold saving on the number of messages sent. Also, our algorithms are significantly simpler to implement compared to, for instance, the GHS algorithm, which is essentially optimal with regards to the message complexity. Thus, our results

  13. ARM Climate Research Facility Spectral Surface Albedo Value-Added Product (VAP) Report

    SciTech Connect (OSTI)

    McFarlane, S; Gaustad, K; Long, C; Mlawer, E

    2011-07-15

    This document describes the input requirements, output data products, and methodology for the Spectral Surface Albedo (SURFSPECALB) value-added product (VAP). The SURFSPECALB VAP produces a best-estimate near-continuous high spectral resolution albedo data product using measurements from multifilter radiometers (MFRs). The VAP first identifies best estimates for the MFR downwelling and upwelling shortwave irradiance values, and then calculates narrowband spectral albedo from these best-estimate irradiance values. The methodology for finding the best-estimate values is based on a simple process of screening suspect data and backfilling screened and missing data with estimated values when possible. The resulting best-estimate MFR narrowband spectral albedos are used to determine a daily surface type (snow, 100% vegetation, partial vegetation, or 0% vegetation). For non-snow surfaces, a piecewise continuous function is used to estimate a high spectral resolution albedo at 1 min temporal and 10 cm-1 spectral resolution.

  14. Hamiltonian Light-front Field Theory Within an AdS/QCD Basis

    SciTech Connect (OSTI)

    Vary, J.P.; Honkanen, H.; Li, Jun; Maris, P.; Brodsky, S.J.; Harindranath, A.; de Teramond, G.F.; Sternberg, P.; Ng, E.G.; Yang, C.; /LBL, Berkeley

    2009-12-16

    Non-perturbative Hamiltonian light-front quantum field theory presents opportunities and challenges that bridge particle physics and nuclear physics. Fundamental theories, such as Quantum Chromodynamics (QCD) and Quantum Electrodynamics (QED) offer the promise of great predictive power spanning phenomena on all scales from the microscopic to cosmic scales, but new tools that do not rely exclusively on perturbation theory are required to make connection from one scale to the next. We outline recent theoretical and computational progress to build these bridges and provide illustrative results for nuclear structure and quantum field theory. As our framework we choose light-front gauge and a basis function representation with two-dimensional harmonic oscillator basis for transverse modes that corresponds with eigensolutions of the soft-wall AdS/QCD model obtained from light-front holography.

  15. System for adding sulfur to a fuel cell stack system for improved fuel cell stability

    DOE Patents [OSTI]

    Mukerjee, Subhasish; Haltiner, Jr., Karl J; Weissman, Jeffrey G.

    2012-03-06

    A system for adding sulfur to a fuel cell stack, having a reformer adapted to reform a hydrocarbon fuel stream containing sulfur contaminants, thereby providing a reformate stream having sulfur; a sulfur trap fluidly coupled downstream of the reformer for removing sulfur from the reformate stream, thereby providing a desulfurized reformate stream; and a metering device in fluid communication with the reformate stream upstream of the sulfur trap and with the desulfurized reformate stream downstream of the sulfur trap. The metering device is adapted to bypass a portion of the reformate stream to mix with the desulfurized reformate stream, thereby producing a conditioned reformate stream having a predetermined sulfur concentration that gives an acceptable balance of minimal drop in initial power with the desired maximum stability of operation over prolonged periods for the fuel cell stack.

  16. Value-Added Products From FGD Sulfite-Rich Scrubber Materials

    SciTech Connect (OSTI)

    Vivak M. Malhotra

    2006-09-30

    Massive quantities of sulfite-rich flue gas desulfurization (FGD) scrubber materials are produced every year in the USA. In fact, at present, the production of wet sulfite-rich scrubber cake outstrips the production of wet sulfate-rich scrubber cake by about 6 million tons per year. However, most of the utilization focus has centered on FGD gypsum. Therefore, we have recently initiated research on developing new strategies for the economical, but environmentally-sound, utilization of sulfite-rich scrubber material. In this exploratory project (Phase I), we attempted to ascertain whether it is feasible to develop reconstituted wood replacement products from sulfite-rich scrubber material. In pursuit of this goal, we characterized two different wet sulfite-rich scrubber materials, obtained from two power plants burning Midwestern coal, for their suitability for the development of value-added products. The overall strategy adopted was to fabricate composites where the largest ingredient was scrubber material with additional crop materials as additives. Our results suggested that it may be feasible to develop composites with flexural strength as high as 40 MPa (5800 psi) without the addition of external polymers. We also attempted to develop load-bearing composites from scrubber material, natural fibers, and phenolic polymer. The polymer-to-solid ratio was limited to {le} 0.4. The formulated composites showed flexural strengths as high as 73 MPa (10,585 psi). We plan to harness the research outcomes from Phase I to develop parameters required to upscale our value-added products in Phase II.

  17. Gas bubble disease: introduction

    SciTech Connect (OSTI)

    Fickeisen, D.H.; Schneider, M.J.; Wedemeyer, G.A.

    1980-11-01

    In 1970, gas bubble disease was identified as a serious problem affecting salmonids in the Columbia and Snake river systems. The source of supersaturation was entrainment of air into water spilling over hydroelectric dams. Regional research projects focusing on tolerance bioassays were immediately implemented. Since then, the scope of gas bubble disease research has broadened to include problems in other aquatic systems, with other species. Emphasis has shifted from defining tolerance limits in bioassay systems to exploring behavioral and physiological aspects. Various methods of degasifying supersaturated water have been developed.

  18. Using Wikipedia to forecast diseases

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Using Wikipedia to forecast diseases Using Wikipedia to forecast diseases Scientists can now monitor and forecast diseases around the globe more effectively by analyzing views of Wikipedia articles. November 13, 2014 Del Valle and her team observe findings from their research on disease patterns from analyzing Wikipedia articles. Del Valle and her team observe findings from their research on disease patterns from analyzing Wikipedia articles. Contact Nancy Ambrosiano Communications Office (505)

  19. Virtual machine-based simulation platform for mobile ad-hoc network-based cyber infrastructure

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Yoginath, Srikanth B.; Perumalla, Kayla S.; Henz, Brian J.

    2015-09-29

    In modeling and simulating complex systems such as mobile ad-hoc networks (MANETs) in de-fense communications, it is a major challenge to reconcile multiple important considerations: the rapidity of unavoidable changes to the software (network layers and applications), the difficulty of modeling the critical, implementation-dependent behavioral effects, the need to sustain larger scale scenarios, and the desire for faster simulations. Here we present our approach in success-fully reconciling them using a virtual time-synchronized virtual machine(VM)-based parallel ex-ecution framework that accurately lifts both the devices as well as the network communications to a virtual time plane while retaining full fidelity. At themore » core of our framework is a scheduling engine that operates at the level of a hypervisor scheduler, offering a unique ability to execute multi-core guest nodes over multi-core host nodes in an accurate, virtual time-synchronized manner. In contrast to other related approaches that suffer from either speed or accuracy issues, our framework provides MANET node-wise scalability, high fidelity of software behaviors, and time-ordering accuracy. The design and development of this framework is presented, and an ac-tual implementation based on the widely used Xen hypervisor system is described. Benchmarks with synthetic and actual applications are used to identify the benefits of our approach. The time inaccuracy of traditional emulation methods is demonstrated, in comparison with the accurate execution of our framework verified by theoretically correct results expected from analytical models of the same scenarios. In the largest high fidelity tests, we are able to perform virtual time-synchronized simulation of 64-node VM-based full-stack, actual software behaviors of MANETs containing a mix of static and mobile (unmanned airborne vehicle) nodes, hosted on a 32-core host, with full fidelity of unmodified ad-hoc routing protocols, unmodified

  20. Virtual machine-based simulation platform for mobile ad-hoc network-based cyber infrastructure

    SciTech Connect (OSTI)

    Yoginath, Srikanth B.; Perumalla, Kayla S.; Henz, Brian J.

    2015-09-29

    In modeling and simulating complex systems such as mobile ad-hoc networks (MANETs) in de-fense communications, it is a major challenge to reconcile multiple important considerations: the rapidity of unavoidable changes to the software (network layers and applications), the difficulty of modeling the critical, implementation-dependent behavioral effects, the need to sustain larger scale scenarios, and the desire for faster simulations. Here we present our approach in success-fully reconciling them using a virtual time-synchronized virtual machine(VM)-based parallel ex-ecution framework that accurately lifts both the devices as well as the network communications to a virtual time plane while retaining full fidelity. At the core of our framework is a scheduling engine that operates at the level of a hypervisor scheduler, offering a unique ability to execute multi-core guest nodes over multi-core host nodes in an accurate, virtual time-synchronized manner. In contrast to other related approaches that suffer from either speed or accuracy issues, our framework provides MANET node-wise scalability, high fidelity of software behaviors, and time-ordering accuracy. The design and development of this framework is presented, and an ac-tual implementation based on the widely used Xen hypervisor system is described. Benchmarks with synthetic and actual applications are used to identify the benefits of our approach. The time inaccuracy of traditional emulation methods is demonstrated, in comparison with the accurate execution of our framework verified by theoretically correct results expected from analytical models of the same scenarios. In the largest high fidelity tests, we are able to perform virtual time-synchronized simulation of 64-node VM-based full-stack, actual software behaviors of MANETs containing a mix of static and mobile (unmanned airborne vehicle) nodes, hosted on a 32-core host, with full fidelity of unmodified ad-hoc routing protocols, unmodified application

  1. Semiclassical circular strings in AdS{sub 5} and 'long' gauge field strength operators

    SciTech Connect (OSTI)

    Park, I.Y.; Tirziu, A.; Tseytlin, A.A.

    2005-06-15

    We consider circular strings rotating with equal spins S{sub 1}=S{sub 2}=S in two orthogonal planes in AdS{sub 5} and suggest that they may be dual to long gauge-theory operators built out of self-dual components of gauge field strength. As was found in hep-th/0404187, the one-loop anomalous dimensions of the such gauge-theory operators are described by an antiferromagnetic XXX{sub 1} spin chain and scale linearly with length L>>1. We find that in the case of rigid rotating string both the classical energy E{sub 0} and the 1-loop string correction E{sub 1} depend linearly on the spin S (within the stability region of the solution). This supports the identification of the rigid rotating string with the gauge-theory operator corresponding to the maximal-spin (ferromagnetic) state of the XXX{sub 1} spin chain. The energy of more general rotating and pulsating strings also happens to scale linearly with both the spin and the oscillation number. Such solutions should be dual to other lower-spin states of the spin chain, with the antiferromagnetic ground state presumably corresponding to the string pulsating in two planes with no rotation.

  2. A Time-Slotted On-Demand Routing Protocol for Mobile Ad Hoc Unmanned Vehicle Systems

    SciTech Connect (OSTI)

    Hope Forsmann; Robert Hiromoto; John Svoboda

    2007-04-01

    The popularity of UAVs has increased dramatically because of their successful deployment in military operations, their ability to preserve human life, and the continual improvements in wireless communication that serves to increase their capabilities. We believe the usefulness of UAVs would be dramatically increased if formation flight were added to the list of capabilities. Currently, sustained formation flight with a cluster of UAVs has only been achieved with two nodes by the Multi-UAV Testbed at the Massachusetts Institute of Technology. (Park, 2004) Formation flight is a complex operation requiring the ability to adjust the flight patterns on the fly and correct for wind gusts, terrain, and differences in node equipment. All of which increases the amount of inner node communication. Since one of the problems with MANET communication is network congestion, we believe a first step towards formation flight can be made through improved inner node communication. We have investigated current communication routing protocols and developed an altered hybrid routing protocol in order to provide communication with less network congestion.

  3. Docket # EERE-2014-BT-STD-0051, and/or RIN 1904-AD40 Ex Parte Meeting 24th

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    February 2015 | Department of Energy 4-BT-STD-0051, and/or RIN 1904-AD40 Ex Parte Meeting 24th February 2015 Docket # EERE-2014-BT-STD-0051, and/or RIN 1904-AD40 Ex Parte Meeting 24th February 2015 Thanks for the invitation to discuss and explain comments to a new proposed gas compressor efficiency standard. DoE GasCompressors Efficiency HOERBIGER 9-1-2015 AdditionalResponse (83.97 KB) More Documents & Publications Natural Gas Infrastructure R&D and Methane Emissions Mitigation

  4. ARM Climate Research Facility Quarterly Value-Added Product Report January 1–March 30, 2011

    SciTech Connect (OSTI)

    Sivaraman, C

    2011-06-14

    The purpose of this report is to provide a concise status update for value-added products (VAP) implemented by the Atmospheric Radiation Measurement Climate Research Facility. The report is divided into the following sections: (1) new VAPs for which development has begun, (2) progress on existing VAPs, and (3) future VAPs that have been recently approved.

  5. Strings on AdS wormholes

    SciTech Connect (OSTI)

    Ali, Mir; Ruiz, Frenny; Saint-Victor, Carlos; Vazquez-Poritz, Justin F.

    2009-08-15

    We consider the behavior of open strings on anti-de Sitter wormholes in Gauss-Bonnet theory, which are the Gauss-Bonnet gravity duals of a pair of field theories. A string with both endpoints on the same side of the wormhole describes two charges within the same field theory, which exhibit Coulomb interaction for small separation. On the other hand, a string extending through the wormhole describes two charges which live in different field theories, and they exhibit a springlike confining potential. A transition occurs when there is a pair of charges present within each field theory: for small separation each pair of charges exhibits Coulomb interaction, while for large separation the charges in the different field theories pair up and exhibit confinement. Two steadily-moving charges in different field theories can occupy the same location provided that their speed is less than a critical speed, which also plays the role of a subluminal speed limit. However, for some wormhole backgrounds, charges moving at the critical speed cannot occupy the same location and energy is transferred from the leading charge to the lagging one. We also show that strings on anti-de Sitter wormholes in supergravity theories without higher-derivative curvature terms can exhibit these properties as well.

  6. ARM Climate Research Facility Quarterly Value-Added Product Report First Quarter: October 01-December 31, 2011

    SciTech Connect (OSTI)

    Sivaraman, C

    2012-02-28

    The purpose of this report is to provide a concise status update for value-added products (VAP) implemented by the Atmospheric Radiation Measurement (ARM) Climate Research Facility. The report is divided into the following sections: (1) new VAPs for which development has begun, (2) progress on existing VAPs, (3) future VAPs that have been recently approved, (4) other work that leads to a VAP, and (5) top requested VAPs from the archive.

  7. ARM Climate Research Facility Quarterly Value-Added Product Report Fourth Quarter: July 1–September 30, 2012

    SciTech Connect (OSTI)

    Sivaraman, C

    2012-11-13

    The purpose of this report is to provide a concise status update for value-added products (VAP) implemented by the Atmospheric Radiation Measurement (ARM) Climate Research Facility. The report is divided into the following sections: (1) new VAPs for which development has begun, (2) progress on existing VAPs, (3) future VAPs that have been recently approved, (4) other work that leads to a VAP, and (5) top requested VAPs from the archive.

  8. ARM Climate Research Facility Quarterly Value-Added Product Report Third Quarter: April 01–June 30, 2011

    SciTech Connect (OSTI)

    Sivaraman, C

    2011-08-18

    The purpose of this report is to provide a concise status update for value-added products (VAP) implemented by the Atmospheric Radiation Measurement Climate Research Facility. The report is divided into the following sections: (1) new VAPs for which development has begun, (2) progress on existing VAPs, (3) future VAPs that have been recently approved, (4) other work that leads to a VAP, and (5) top requested VAPs from the archive

  9. DOE/SC-ARM/TR-095 The Microbase Value-Added Product: A Baseline Retrieval of Cloud

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    5 The Microbase Value-Added Product: A Baseline Retrieval of Cloud Microphysical Properties M Dunn K Johnson M Jensen May 2011 DISCLAIMER This report was prepared as an account of work sponsored by the U.S. Government. Neither the United States nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or

  10. Top Value Added Chemicals from Biomass: Volume I--Results of Screening for Potential Candidates from Sugars and Synthesis Gas

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Top Value Added Chemicals from Biomass Volume I-Results of Screening for Potential Candidates from Sugars and Synthesis Gas Produced by the Staff at Pacific Northwest National Laboratory (PNNL) National Renewable Energy Laboratory (NREL) Office of Biomass Program (EERE) For the Office of the Biomass Program T. Werpy and G. Petersen, Editors U.S. Department of Energy Energy Efficiency and Renewable Energy Bringing you a prosperous future where energy is clean, abundant, reliable, and affordable

  11. Effect of unmodulated laser light on the nanostructure of a thin solid AD-1 azo dye film

    SciTech Connect (OSTI)

    Dubrovkin, A M; Ezhov, A A; Kozenkov, V M; Magnitskiy, Sergey A; Nagorskiy, Nikolay M; Panov, Vladimir I

    2010-06-23

    Exposure to light uniform in intensity and polarisation causes marked changes in the surface topography of a thin (320 nm) nanostructured AD-1 low molecular weight azo dye film. Linearly polarised incoherent light with a wavelength of 470 nm and intensity of 1 mW cm{sup -2} produces numerous teardrop-shaped hillocks of the order of 200 nm in radius over most of the film surface. (letters)

  12. Chapter 4: Advancing Clean Electric Power Technologies | Carbon Dioxide and Storage Value-Added Options Technology Assessment

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Storage Value-Added Options Carbon Dioxide Capture for Natural Gas and Industrial Applications Carbon Dioxide Capture Technologies Carbon Dioxide Storage Technologies Crosscutting Technologies in Carbon Dioxide Capture and Storage Fast-spectrum Reactors Geothermal Power High Temperature Reactors Hybrid Nuclear-Renewable Energy Systems Hydropower Light Water Reactors Marine and Hydrokinetic Power Nuclear Fuel Cycles Solar Power Stationary Fuel Cells Supercritical Carbon Dioxide Brayton Cycle

  13. Possible mechanisms for arsenic-induced proliferative diseases

    SciTech Connect (OSTI)

    Wetterhahn, K.E.; Dudek, E.J.; Shumilla, J.A.

    1996-12-31

    Possible mechanisms for cardiovascular diseases and cancers which have been observed on chronic exposure to arsenic have been investigated. We tested the hypothesis that nonlethal levels of arsenic are mitogenic, cause oxidative stress, increase nuclear translocation of trans-acting factors, and increase expression of genes involved in proliferation. Cultured porcine vascular (from aorta) endothelial cells were used as a model cell system to study the effects of arsenic on the target cells for cardiovascular diseases. Treatment of postconfluent cell cultures with nonovertly toxic concentrations of arsenite increased DNA synthesis, similar to the mitogenic response observed with hydrogen peroxide. Within 1 hour of adding noncytotoxic concentrations of arsenite, cellular levels of oxidants increased relative to control levels, indicating that arsenite promotes cellular oxidations. Arsenite treatment increased nuclear translocation of NF-{kappa}B, an oxidative stress-responsive transcription factor, in a manner similar to that observed with hydrogen peroxide. Pretreatment of intact cells with the antioxidants N-acetylcysteine and dimethylfumarate prevented the arsenite-induced increases in cellular oxidant formation and NF-KB translocation. Arsenite had little or no effect on binding of NF-KB to its DNA recognition sequence in vitro, indicating that it is unlikely that arsenite directly affects NF-KB. The steady-state mRNA levels of intracellular adhesion molecule and urokinase-like plasminogen activator, genes associated with the active endothelial phenotype in arteriosclerosis and cancer metastasis, were increased by nontoxic concentrations of arsenite. These data suggest that arsenite promotes proliferative diseases like heart disease and cancer by activating oxidant-sensitive endothelial cell signaling and gene expression. It is possible that antioxidant therapy would be useful in preventing arsenic-induced cardiovascular disease and cancer.

  14. OSTIblog Articles in the disease Topic | OSTI, US Dept of Energy...

    Office of Scientific and Technical Information (OSTI)

    John Q. Trojanowski, an Alzheimer's researcher at the University of Pennsylvania, "It's not science the way most of us have practiced it in our careers. But we all realized that we ...

  15. Adding Erlotinib to Chemoradiation Improves Overall Survival but Not Progression-Free Survival in Stage III Non-Small Cell Lung Cancer

    SciTech Connect (OSTI)

    Komaki, Ritsuko; Allen, Pamela K.; Wei, Xiong; Blumenschein, George R.; Tang, Ximing; Lee, J. Jack; Welsh, James W.; Wistuba, Ignacio I.; Liu, Diane D.; Hong, Waun Ki

    2015-06-01

    Purpose: To test, in a single-arm, prospective, phase 2 trial, whether adding the epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor erlotinib to concurrent chemoradiotherapy for previously untreated, locally advanced, inoperable non-small cell lung cancer would improve survival and disease control without increasing toxicity. Methods and Materials: Forty-eight patients with previously untreated non-small cell lung cancer received intensity modulated radiation therapy (63 Gy/35 fractions) on Monday through Friday, with chemotherapy (paclitaxel 45 mg/m², carboplatin area under the curve [AUC] = 2) on Mondays, for 7 weeks. All patients also received the EGFR tyrosine kinase inhibitor erlotinib (150 mg orally 1/d) on Tuesday-Sunday for 7 weeks, followed by consolidation paclitaxel–carboplatin. The primary endpoint was time to progression; secondary endpoints were overall survival (OS), toxicity, response, and disease control and whether any endpoint differed by EGFR mutation status. Results: Of 46 patients evaluable for response, 40 were former or never-smokers, and 41 were evaluable for EGFR mutations (37 wild-type [WT] and 4 mutated [all adenocarcinoma]). Median time to progression was 14.0 months and did not differ by EGFR status. Toxicity was acceptable (no grade 5, 1 grade 4, 11 grade 3). Twelve patients (26%) had complete responses (10 WT, 2 mutated), 27 (59%) partial (21 WT, 2 mutated, 4 unknown), and 7 (15%) none (6 WT, 2 mutated, 1 unknown) (P=.610). At 37.0 months' follow-up (range, 3.6-76.5 months) for all patients, median OS time was 36.5 months, and 1-, 2-, and 5-year OS rates were 82.6%, 67.4%, and 35.9%, respectively; none differed by mutation status. Twelve patients had no progression, and 34 had local and/or distant failure. Eleven of 27 distant failures were in the brain (7 WT, 3 mutated, 1 unknown). Conclusions: Toxicity and OS were promising, but time to progression did not meet expectations. The prevalence of distant

  16. Atomic-resolution 3D structure of amyloid β fibrils: The Osaka mutation

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Schutz, Anne K.; Wall, Joseph; Vagt, Toni; Huber, Matthias; Ovchinnikova, Oxana Y.; Cadalbert, Riccardo; Guntert, Peter; Bockmann, Anja; Glockshuber, Rudi; Meier, Beat H.

    2014-11-13

    Despite its central importance for understanding the molecular basis of Alzheimer's disease (AD), high-resolution structural information on amyloid β-peptide (Aβ) fibrils, which are intimately linked with AD, is scarce. We report an atomic-resolution fibril structure of the Aβ 1-40 peptide with the Osaka mutation (E22Δ), associated with early-onset AD. The structure, which differs substantially from all previously proposed models, is based on a large number of unambiguous intra- and intermolecular solid-state NMR distance restraints

  17. Atomic-resolution 3D structure of amyloid ? fibrils: The Osaka mutation

    SciTech Connect (OSTI)

    Schutz, Anne K.; Wall, Joseph; Vagt, Toni; Huber, Matthias; Ovchinnikova, Oxana Y.; Cadalbert, Riccardo; Guntert, Peter; Bockmann, Anja; Glockshuber, Rudi; Meier, Beat H.

    2014-11-13

    Despite its central importance for understanding the molecular basis of Alzheimer's disease (AD), high-resolution structural information on amyloid ?-peptide (A?) fibrils, which are intimately linked with AD, is scarce. We report an atomic-resolution fibril structure of the A? 1-40 peptide with the Osaka mutation (E22?), associated with early-onset AD. The structure, which differs substantially from all previously proposed models, is based on a large number of unambiguous intra- and intermolecular solid-state NMR distance restraints

  18. Investigating the Influence of the Added Mass Effect to Marine Hydrokinetic Horizontal-Axis Turbines Using a General Dynamic Wake Wind Turbine Code: Preprint

    SciTech Connect (OSTI)

    Maniaci, D. C.; Li, Y.

    2012-04-01

    This paper describes a recent study to investigate the applicability of a horizontal-axis wind turbine (HAWT) structural dynamics and unsteady aerodynamics analysis program (FAST and AeroDyn respectively) to modeling the forces on marine hydrokinetic (MHK) turbines. It summarizes the added mass model that has been added to AeroDyn. The added mass model only includes flow acceleration perpendicular to the rotor disc, and ignores added mass forces caused by blade deflection. A model of the National Renewable Energy Laboratory's (NREL) Unsteady Aerodynamics Experiment (UAE) Phase VI wind turbine was analyzed using FAST and AeroDyn with sea water conditions and the new added mass model. The results of this analysis exhibited a 3.6% change in thrust for a rapid pitch case and a slight change in amplitude and phase of thrust for a case with 30 degrees of yaw.

  19. Investigating the Influence of the Added Mass Effect to Marine Hydrokinetic Horizontal-Axis Turbines Using a General Dynamic Wake Wind Turbine Code

    SciTech Connect (OSTI)

    Maniaci, D. C.; Li, Y.

    2011-10-01

    This paper describes a recent study to investigate the applicability of a horizontal-axis wind turbine (HAWT) structural dynamics and unsteady aerodynamics analysis program (FAST and AeroDyn respectively) to modeling the forces on marine hydrokinetic (MHK) turbines. This paper summarizes the added mass model that has been added to AeroDyn. The added mass model only includes flow acceleration perpendicular to the rotor disc, and ignores added mass forces caused by blade deflection. A model of the National Renewable Energy Laboratory's (NREL) Unsteady Aerodynamics Experiment (UAE) Phase VI wind turbine was analyzed using FAST and AeroDyn with sea water conditions and the new added mass model. The results of this analysis exhibited a 3.6% change in thrust for a rapid pitch case and a slight change in amplitude and phase of thrust for a case with 30{sup o} of yaw.

  20. Early detection of contagious diseases

    DOE Patents [OSTI]

    Colston, Jr., Billy W.; Milanovich, Fred P.; Estacio, Pedro; Chang, John

    2011-08-09

    This invention provides an electronic proximity apparatus and a surveillance method using such an apparatus for alerting individuals that are exposed to a contagious disease. When a person becomes symptomatic and is diagnosed as positive for a given contagious agent, individuals that have recently maintained a threshold proximity with respect to an infected individual are notified and advised to seek immediate medial care. Treatment of individuals in the very early phases of infection (pre-symptomatic) significantly reduces contagiousness of the infected population first exposed to the contagious disease, thus preventing spread of the disease throughout the general population.

  1. ARM Climate Research Facility Quarterly Value-Added Product Report Fourth Quarter: July 01–September 30, 2011

    SciTech Connect (OSTI)

    Sivaraman, C

    2011-11-02

    The purpose of this report is to provide a concise status update for value-added products (VAP) implemented by the Atmospheric Radiation Measurement Climate Research Facility. The report is divided into the following sections: (1) new VAPs for which development has begun, (2) progress on existing VAPs, (3) future VAPs that have been recently approved, (4) other work that leads to a VAP, and (5) top requested VAPs from the archive. New information is highlighted in blue text. New information about processed data by the developer is highlighted in red text.

  2. On N = 2 compactifications of M-theory to AdS{sub 3} using geometric algebra techniques

    SciTech Connect (OSTI)

    Babalic, E. M.; Coman, I. A.; Condeescu, C.; Micu, A.; Lazaroiu, C. I.

    2013-11-13

    We investigate the most general warped compactification of eleven-dimensional supergravity on eight-dimensional manifolds to AdS{sub 3} spaces (in the presence of non-vanishing four-form flux) which preserves N = 2 supersymmetry in three dimensions. Without imposing any restrictions on the chirality of the internal part of the supersymmetry generators, we use geometric algebra techniques to study some implications of the supersymmetry constraints. In particular, we discuss the Lie bracket of certain vector fields constructed as pinor bilinears on the compactification manifold.

  3. DOE/SC-ARM/P-07-005.1 ARM Value-Added Product (VAP) Monthly Status Report

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 ARM Value-Added Product (VAP) Monthly Status Report ARM Translator Team J. Comstock C. Flynn M. Jensen C. Long D. Turner S. Xie March 13, 2007 Work supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research DISCLAIMER This report was prepared as an account of work sponsored by the U.S. Government. Neither the United States nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal

  4. No-carrier-added (NCA) aryl (18E) fluorides via the nucleophilic aromatic substitution of electron rich aromatic rings

    DOE Patents [OSTI]

    Ding, Yu-Shin; Fowler, Joanna S.; Wolf, Alfred P.

    1993-01-01

    A method for synthesizing no-carrier-added (NCA) aryl [.sup.18 F] fluoride substituted aromatic aldehyde compositions bearing an electron donating group is described. The method of the present invention includes the step of reacting aromatic nitro aldehydes having a suitably protected hydroxyl substitutent on an electron rich ring. The reaction is The U.S. Government has rights in this invention pursuant to Contract Number DE-AC02-76CH00016, between the U.S. Department of Energy and Associated Universities Inc.

  5. No-carrier-added (NCA) aryl ([sup 18]F) fluorides via the nucleophilic aromatic substitution of electron rich aromatic rings

    DOE Patents [OSTI]

    Yushin Ding; Fowler, J.S.; Wolf, A.P.

    1993-10-19

    A method for synthesizing no-carrier-added (NCA) aryl [.sup.18 F] fluoride substituted aromatic aldehyde compositions bearing an electron donating group is described. The method of the present invention includes the step of reacting aromatic nitro aldehydes having a suitably protected hydroxyl substitutent on an electron rich ring. The reaction is The U.S. Government has rights in this invention pursuant to Contract Number DE-AC02-76CH00016, between the U.S. Department of Energy and Associated Universities Inc.

  6. Top Value-Added Chemicals from Biomass - Volume II„Results of Screening for Potential Candidates from Biorefinery Lignin

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Top Value-Added Chemicals from Biomass Volume II-Results of Screening for Potential Candidates from Biorefinery Lignin 1 JE Holladay 2 JJ Bozell 1 JF White 3 D Johnson 1 Pacific Northwest National Laboratory 2 University of Tennessee 3 National Renewable Energy Laboratory October 2007 Prepared for the U.S. Department of Energy under Contract DE-AC05-76RL01830 PNNL-16983 DISCLAIMER This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the

  7. Broad Temperature Pinning Study of 15 mol.% Zr-Added (Gd, Y)-Ba-Cu-O MOCVD Coated Conductors

    SciTech Connect (OSTI)

    Xu, AX; Khatri, N; Liu, YH; Majkic, G; Galstyan, E; Selvamanickam, V; Chen, YM; Lei, CH; Abraimov, D; Hu, XB; Jaroszynski, J; Larbalestier, D

    2015-06-01

    BaZrO3 (BZO) nanocolumns have long been shown to be very effective for raising the pinning force F-p of REBa2Cu3Ox (REBCO, where RE = rare earth) films at high temperatures and recently at low temperatures too. We have successfully incorporated a high density of BZO nanorods into metal organic chemical vapor deposited (MOCVD) REBCO coated conductors via Zr addition. We found that, compared to the 7.5% Zr-added coated conductor, dense BZO nanorod arrays in the 15% Zr-added conductor are effective over the whole temperature range from 77 K down to 4.2 K. We attribute the substantially enhanced J(c) at 30 K to the weak uncorrelated pinning as well as the strong correlated pinning. Meanwhile, by tripling the REBCO layer thickness to similar to 2.8 mu m, the engineering critical current density J(e) at 30 K exceeds J(e) of optimized Nb-Ti wires at 4.2 K.

  8. Microalgae as a feedstock for biofuel precursors and value-added products: Green fuels and golden opportunities

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Tang, Yuting; Rosenberg, Julian N.; Bohutskyi, Pavlo; Yu, Geng; Betenbaugh, Michael J.; Wang, Fei

    2015-11-16

    In this study, the prospects of biofuel production from microalgal carbohydrates and lipids coupled with greenhouse gas mitigation due to photosynthetic assimilation of CO2 have ushered in a renewed interest in algal feedstock. Furthermore, microalgae (including cyanobacteria) have become established as commercial sources of value-added biochemicals such as polyunsaturated fatty acids and carotenoid pigments used as antioxidants in nutritional supplements and cosmetics. This article presents a comprehensive synopsis of the metabolic basis for accumulating lipids as well as applicable methods of lipid and cellulose bioconversion and final applications of these natural or refined products from microalgal biomass. For lipids, one-stepmore » in situ transesterification offers a new and more accurate approach to quantify oil content. As a complement to microalgal oil fractions, the utilization of cellulosic biomass from microalgae to produce bioethanol by fermentation, biogas by anaerobic digestion, and bio-oil by hydrothermal liquefaction are discussed. Collectively, a compendium of information spanning green renewable fuels and value-added nutritional compounds is provided.« less

  9. Microalgae as a feedstock for biofuel precursors and value-added products: Green fuels and golden opportunities

    SciTech Connect (OSTI)

    Tang, Yuting; Rosenberg, Julian N.; Bohutskyi, Pavlo; Yu, Geng; Betenbaugh, Michael J.; Wang, Fei

    2015-11-16

    In this study, the prospects of biofuel production from microalgal carbohydrates and lipids coupled with greenhouse gas mitigation due to photosynthetic assimilation of CO2 have ushered in a renewed interest in algal feedstock. Furthermore, microalgae (including cyanobacteria) have become established as commercial sources of value-added biochemicals such as polyunsaturated fatty acids and carotenoid pigments used as antioxidants in nutritional supplements and cosmetics. This article presents a comprehensive synopsis of the metabolic basis for accumulating lipids as well as applicable methods of lipid and cellulose bioconversion and final applications of these natural or refined products from microalgal biomass. For lipids, one-step in situ transesterification offers a new and more accurate approach to quantify oil content. As a complement to microalgal oil fractions, the utilization of cellulosic biomass from microalgae to produce bioethanol by fermentation, biogas by anaerobic digestion, and bio-oil by hydrothermal liquefaction are discussed. Collectively, a compendium of information spanning green renewable fuels and value-added nutritional compounds is provided.

  10. The Y-12 Plant No Rad-Added Program for off-site shipment of nonradioactive hazardous waste

    SciTech Connect (OSTI)

    Cooper, K.H.; Mattie, B.K.; Williams, J.L.; Jacobs, D.G.; Roberts, K.A.

    1994-02-14

    On May 17, 1991, the US Department of Energy (DOE) issued a directive for DOE operations to cease off-site shipments of non-radioactive hazardous waste pending further clarification and approvals. A DOE Performance Objective for Certification of Non-Radioactive Hazardous Waste was issued in November 1991. In response to these directives, the Waste Management Division of Oak Ridge Y-12 Plant, with assistance from Roy F. Weston, Inc., has developed a No Rad-Added Program to provide small programmatic guidance and a set of procedures, approved by DOE, which will permit hazardous waste to be shipped from the Y-12 Plant to commercial treatment, storage, or disposal facilities after ensuring and certifying that hazardous waste has no radioactivity added as a result of DOE operations. There are serious legal and financial consequences of shipping waste containing radioactivity to an off-site facility not licensed to receive radioactive materials. Therefore, this program is designed with well-defined responsibilities and stringent documentation requirements.

  11. Data:5277c4c2-0b8b-4179-ad2c-59139826c6f2 | Open Energy Information

    Open Energy Info (EERE)

    ad2c-59139826c6f2 No revision has been approved for this page. It is currently under review by our subject matter experts. Jump to: navigation, search Loading... 1. Basic...

  12. Data:9f33a9f9-21ad-47b2-9b1f-4b3771f914ac | Open Energy Information

    Open Energy Info (EERE)

    ad-47b2-9b1f-4b3771f914ac No revision has been approved for this page. It is currently under review by our subject matter experts. Jump to: navigation, search Loading... 1. Basic...

  13. Preclinical pharmacology and toxicology study of Ad-hTERT-E1a-Apoptin, a novel dual cancer-specific oncolytic adenovirus

    SciTech Connect (OSTI)

    Qi, Yanxin; Guo, Huanhuan; Hu, Ningning; He, Dongyun; Zhang, Shi; Chu, Yunjie; Huang, Yubin; Li, Xiao; Sun, LiLi; Jin, Ningyi

    2014-10-15

    Clinical studies have demonstrated that conditionally replicating adenovirus is safe. We constructed an oncolytic adenovirus, Ad-hTERT-E1a-Apoptin, using a cancer-specific promoter (human telomerase reverse transcriptase promoter, hTERTp) and a cancer cell-selective apoptosis-inducing gene (Apoptin). Ad-hTERT-E1a-Apoptin was proven effective both in vitro and in vivo in our previous study. In this study, the preclinical safety profiles of Ad-hTERT-E1a-Apoptin in animal models were investigated. At doses of 5.0 × 10{sup 8}, 2.5 × 10{sup 9}, and 1.25 × 10{sup 10} viral particles (VP)/kg, Ad-hTERT-E1a-Apoptin had no adverse effects on mouse behavior, muscle cooperation, sedative effect, digestive system, and nervous systems, or on beagle cardiovascular and respiratory systems at 5.0 × 10{sup 8}, 2.5 × 10{sup 9}, and 1.25 × 10{sup 10} VP/kg doses. In acute toxicity tests in mice, the maximum tolerated dose > 5 × 10{sup 10} VP/kg. There was no inflammation or ulceration at the injection sites within two weeks. In repeat-dose toxicological studies, the no observable adverse effect levels of Ad-hTERT-E1a-Apoptin in rats (1.25 × 10{sup 10} VP/kg) and beagles (2.5 × 10{sup 9} VP/kg) were 62.5- and 12.5-fold of the proposed clinical dose, respectively. The anti-virus antibody was produced in animal sera. Bone marrow examination revealed no histopathological changes. Guinea pigs sensitized by three repeated intraperitoneal injections of 1.35 × 10{sup 10} VP/mL Ad-hTERT-E1a-Apoptin each and challenged by one intravenous injection of 1.67 × 10{sup 8} VP/kg Ad-hTERT-E1a-Apoptin did not exhibit any sign of systemic anaphylaxis. Our data from different animal models suggest that Ad-hTERT-E1a-Apoptin is a safe anti-tumor therapeutic agent. - Highlights: • We use the rodents and non-rodents animal models to evaluation Ad-hTERT-E1a-Apoptin. • Ad-hTERT-E1a-Apoptin is a safe anti-tumor therapeutic agent. • Demonstrate the safety and feasibility dose of injected Ad

  14. Power production and ADS

    SciTech Connect (OSTI)

    Raja, Rajendran; /Fermilab

    2010-03-01

    We describe the power production process in Accelerator Driven Sub-critical systems employing Thorium-232 and Uranium-238 as fuel and examine the demands on the power of the accelerator required.

  15. Poster_AD.ppt

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    radiative transfer (RT) model with an unconventional propagation kernel, i.e., the term in ... Barker, H. W., B. A. Wielicki, and L. Parker, 1996: A parameterization for computing ...

  16. Del Genio-AD

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Simulated Vertical Structure of Tropical Convective Storms: Implications for GCM Parameterization A. D. Del Genio National Aeronautics and Space Administration Goddard Institute for Space Studies New York Introduction General circulation model (GCM) simulations of equilibrium greenhouse gas-induced climate change suggest that the single largest contributor to uncertainty in cloud feedback is due to changes in the areal extent and cloud water contents (CWC) of mesoscale convective systems (Yao

  17. Adding New Functionality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    If an error dialog box appears, try logging in again. Contact Kelle Smith at kelle.smith@pnl.gov if you need assistance. Readiness Review Requests Pre-Deployment Readiness Review...

  18. 201008ad.cdr

    Office of Legacy Management (LM)

    Environmental Assessment for a Proposed Solar Energy Project near Durango The U.S. ... related to installing a photovoltaic solar electrical-generation system of ...

  19. 201004ad.cdr

    Office of Legacy Management (LM)

    Public to Attend Public Scoping Meeting on Proposed Solar Energy Project Near Durango U.S. ... related to installing a photovoltaic solar electrical generation system of up to 4.5 ...

  20. Patching genes to fight disease

    SciTech Connect (OSTI)

    Holzman, D.

    1990-09-03

    The National Institutes of Health has approved the first gene therapy experiments, one of which will try to cure cancer by bolstering the immune system. The applications of such therapy are limited, but the potential aid to people with genetic diseases is great.

  1. Canonical and kinetic forms of the electromagnetic momentum in an ad hoc quantization scheme for a dispersive dielectric

    SciTech Connect (OSTI)

    Garrison, J.C.; Chiao, R.Y.

    2004-11-01

    An ad hoc quantization scheme for the electromagnetic field in a weakly dispersive, transparent dielectric leads to the definition of canonical and kinetic forms for the momentum of the electromagnetic field in a dispersive medium. The canonical momentum is uniquely defined as the operator that generates spatial translations in a uniform medium, but the quantization scheme suggests two possible choices for the kinetic momentum operator, corresponding to the Abraham or the Minkowski momentum in classical electrodynamics. Another implication of this procedure is that a wave packet containing a single dressed photon travels at the group velocity through the medium. The physical significance of the canonical momentum has already been established by considerations of energy and momentum conservation in the atomic recoil due to spontaneous emission, the Cerenkov effect, the Doppler effect, and phase matching in nonlinear optical processes. In addition, the data of the Jones and Leslie radiation pressure experiment is consistent with the assignment of one ({Dirac_h}/2{pi})k unit of canonical momentum to each dressed photon. By contrast, experiments in which the dielectric is rigidly accelerated by unbalanced electromagnetic forces require the use of the Abraham momentum.

  2. Luminescence and electrical properties of solution-processed ZnO thin films by adding fluorides and annealing atmosphere

    SciTech Connect (OSTI)

    Choi, Sungho; Park, Byung-Yoon; Jung, Ha-Kyun

    2011-06-15

    Highlights: {yields} Systematic study of the fluorides doped solution-processed ZnO thin films via the luminescence and electrical behaviors. {yields} Defect-related visible emission bands are affected by annealing ambient and fluoride addition. {yields} Adding lithium fluoride followed by annealing in oxygen ambient leads to a controlled defect density with proper TFT performance. -- Abstract: To develop an efficient channel layer for thin film transistors (TFTs), understanding the defect-related luminescence and electrical property is crucial for solution-processed ZnO thin films. Film growth with the fluorides addition, especially using LiF, followed by the oxygen ambient post-annealing leads to decreased defect-related emission as well as enhanced switching property. The saturation mobility and current on/off ratio are 0.31 cm{sup 2} V{sup -1} s{sup -1} and 1.04 x 10{sup 3}. Consequently, we can visualize an optimized process condition and characterization method for solution-processed TFT based on the fluorine-doped ZnO film channel layer by considering the overall emission behavior.

  3. Extended Virtual Spring Mesh (EVSM): The Distributed Self-Organizing Mobile Ad Hoc Network for Area Exploration

    SciTech Connect (OSTI)

    Kurt Derr

    2011-12-01

    Mobile Ad hoc NETworks (MANETs) are distributed self-organizing networks that can change locations and configure themselves on the fly. This paper focuses on an algorithmic approach for the deployment of a MANET within an enclosed area, such as a building in a disaster scenario, which can provide a robust communication infrastructure for search and rescue operations. While a virtual spring mesh (VSM) algorithm provides scalable, self-organizing, and fault-tolerant capabilities required by aMANET, the VSM lacks the MANET's capabilities of deployment mechanisms for blanket coverage of an area and does not provide an obstacle avoidance mechanism. This paper presents a new technique, an extended VSM (EVSM) algorithm that provides the following novelties: (1) new control laws for exploration and expansion to provide blanket coverage, (2) virtual adaptive springs enabling the mesh to expand as necessary, (3) adapts to communications disturbances by varying the density and movement of mobile nodes, and (4) new metrics to assess the performance of the EVSM algorithm. Simulation results show that EVSM provides up to 16% more coverage and is 3.5 times faster than VSM in environments with eight obstacles.

  4. A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids

    SciTech Connect (OSTI)

    Zipper, Lauren E.; Aristide, Xavier; Bishop, Dylan P.; Joshi, Ishita; Kharzeev, Julia; Patel, Krishna B.; Santiago, Brianna M.; Joshi, Karan; Dorsinvil, Kahille; Sweet, Robert M.; Soares, Alexei S.

    2014-11-28

    A method is described for using plate lids to reduce evaporation in low-volume vapor-diffusion crystallization experiments. The plate lids contain apertures through which the protein and precipitants were added to different crystallization microplates (the reservoir was filled before fitting the lids). Plate lids were designed for each of these commonly used crystallization microplates. This system minimizes the dehydration of crystallization droplets containing just a few nanolitres of protein and precipitant, and results in more reproducible diffraction from the crystals. For each lid design, changes in the weight of the plates were used to deduce the rate of evaporation under different conditions of temperature, air movement, droplet size and precipitant. For comparison, the state of dehydration was also visually assessed throughout the experiment. Finally, X-ray diffraction methods were used to compare the diffraction of protein crystals that were conventionally prepared against those that were prepared on plates with plate lids. The measurements revealed that the plate lids reduced the rate of evaporation by 63–82%. Crystals grown in 5 nl drops that were set up with plate lids diffracted to higher resolution than similar crystals from drops that were set up without plate lids. Ultimately, the results demonstrate that plate lids can be instrumental for improving few-nanolitre crystallizations.

  5. A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zipper, Lauren E.; Aristide, Xavier; Bishop, Dylan P.; Joshi, Ishita; Kharzeev, Julia; Patel, Krishna B.; Santiago, Brianna M.; Joshi, Karan; Dorsinvil, Kahille; Sweet, Robert M.; et al

    2014-11-28

    A method is described for using plate lids to reduce evaporation in low-volume vapor-diffusion crystallization experiments. The plate lids contain apertures through which the protein and precipitants were added to different crystallization microplates (the reservoir was filled before fitting the lids). Plate lids were designed for each of these commonly used crystallization microplates. This system minimizes the dehydration of crystallization droplets containing just a few nanolitres of protein and precipitant, and results in more reproducible diffraction from the crystals. For each lid design, changes in the weight of the plates were used to deduce the rate of evaporation under differentmore » conditions of temperature, air movement, droplet size and precipitant. For comparison, the state of dehydration was also visually assessed throughout the experiment. Finally, X-ray diffraction methods were used to compare the diffraction of protein crystals that were conventionally prepared against those that were prepared on plates with plate lids. The measurements revealed that the plate lids reduced the rate of evaporation by 63–82%. Crystals grown in 5 nl drops that were set up with plate lids diffracted to higher resolution than similar crystals from drops that were set up without plate lids. Ultimately, the results demonstrate that plate lids can be instrumental for improving few-nanolitre crystallizations.« less

  6. Etiology of gas bubble disease

    SciTech Connect (OSTI)

    Bouck, G.R.

    1980-11-01

    Gas bubble disease is a noninfectious, physically induced process caused by uncompensated hyperbaric pressure of total dissolved gases. When pressure compensation is inadequate, dissolved gases may form emboli (in blood) and emphysema (in tissues). The resulting abnormal physical presence of gases can block blood vessels (hemostasis) or tear tissues, and may result in death. Population mortality is generally skewed, in that the median time to death occurs well before the average time to death. Judged from mortality curves, three stages occur in gas bubble disease: (1) a period of gas pressure equilibrium, nonlethal cavitation, and increasing morbidity; (2) a period of rapid and heavy mortality; and (3) a period of protracted survival, despite lesions, and dysfunction that eventually terminates in total mortality. Safe limits for gas supersaturation depend on species tolerance and on factors that differ among hatcheries and rivers, between continuous and intermittent exposures, and across ranges of temperature and salinity.

  7. RNA simulations boost understanding of retroviral diseases

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    RNA simulations boost understanding of retroviral diseases RNA simulations boost understanding of retroviral diseases New molecular dynamics research into how RNA folds into hairpin-shaped structures called tetraloops could provide important insights into new treatments for retroviral diseases. May 31, 2016 An example of RNA folded into a tetraloop, in this case the "Z-form duplex" under study that could provide medical treatment opportunities for retroviral diseases such as Zika and

  8. Protein Instability and Lou Gehrig's Disease

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Protein Instability and Lou Gehrig's Disease Protein Instability and Lou Gehrig's Disease Print Wednesday, 25 March 2015 00:00 A new study links protein instability with the progression of a lethal degenerative disease: amyotrophic lateral sclerosis (ALS), also known as Lou Gehrig's disease. Using several biophysical techniques as well as small-angle x-ray scattering (SAXS) at the Advanced Light Source, researchers focused on the effects of mutations to a gene coding for a protein called

  9. A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids

    SciTech Connect (OSTI)

    Zipper, Lauren E.; Aristide, Xavier; Bishop, Dylan P.; Joshi, Ishita; Kharzeev, Julia; Patel, Krishna B.; Santiago, Brianna M.; Joshi, Karan; Dorsinvil, Kahille; Sweet, Robert M.; Soares, Alexei S.

    2014-11-28

    This article describes the use of evaporation control lids that are fitted to crystallization plates to improve the reproducibility of trials using as little as 5 nl. The plate lids contain apertures which are large enough for the transfer of protein containing droplets, but small enough to greatly reduce the rate of evaporation during the time needed to prepare the plate. A method is described for using plate lids to reduce evaporation in low-volume vapor-diffusion crystallization experiments. The plate lids contain apertures through which the protein and precipitants were added to different crystallization microplates (the reservoir was filled before fitting the lids). Plate lids were designed for each of these commonly used crystallization microplates. This system minimizes the dehydration of crystallization droplets containing just a few nanolitres of protein and precipitant, and results in more reproducible diffraction from the crystals. For each lid design, changes in the weight of the plates were used to deduce the rate of evaporation under different conditions of temperature, air movement, droplet size and precipitant. For comparison, the state of dehydration was also visually assessed throughout the experiment. Finally, X-ray diffraction methods were used to compare the diffraction of protein crystals that were conventionally prepared against those that were prepared on plates with plate lids. The measurements revealed that the plate lids reduced the rate of evaporation by 63–82%. Crystals grown in 5 nl drops that were set up with plate lids diffracted to higher resolution than similar crystals from drops that were set up without plate lids. The results demonstrate that plate lids can be instrumental for improving few-nanolitre crystallizations.

  10. Computational and experimental study of the effects of adding dimethyl ether and ethanol to nonpremixed ethylene/air flames

    SciTech Connect (OSTI)

    Bennett, Beth Anne V.; McEnally, Charles S.; Pfefferle, Lisa D.; Smooke, Mitchell D.; Colket, Meredith B.

    2009-06-15

    Two sets of axisymmetric laminar coflow flames, each consisting of ethylene/air nonpremixed flames with various amounts (up to 10%) of either dimethyl ether (CH{sub 3}-O-CH{sub 3}) or ethanol (CH{sub 3}-CH{sub 2}-OH) added to the fuel stream, have been examined both computationally and experimentally. Computationally, the local rectangular refinement method, which incorporates Newton's method, is used to solve the fully coupled nonlinear conservation equations on solution-adaptive grids for each flame in two spatial dimensions. The numerical model includes C6 chemical kinetic mechanisms with up to 59 species, detailed transport, and an optically thin radiation submodel. Experimentally, thermocouples are used to measure gas temperatures, and mass spectrometry is used to determine concentrations of over 35 species along the flame centerline. Computational results are examined throughout each flame, and validation of the model occurs through comparison with centerline measurements. Very good agreement is observed for temperature, major species, and several minor species. As the level of additive is increased, temperatures, some major species (CO{sub 2}, C{sub 2}H{sub 2}), flame lengths, and residence times are essentially unchanged. However, peak centerline concentrations of benzene (C{sub 6}H{sub 6}) increase, and this increase is largest when dimethyl ether is the additive. Computational and experimental results support the hypothesis that the dominant pathway to C{sub 6}H{sub 6} formation begins with the oxygenates decomposing into methyl radical (CH{sub 3}), which combines with C2 species to form propargyl (C{sub 3}H{sub 3}), which reacts with itself to form C{sub 6}H{sub 6}. (author)

  11. Iron and aluminum interaction with amyloid-beta peptides associated with Alzheimer’s disease

    SciTech Connect (OSTI)

    Drochioiu, Gabi; Ion, Laura; Murariu, Manuela; Habasescu, Laura

    2014-10-06

    An elevation in the concentration of heavy metal ions in Alzheimer’s disease (AD) brain has been demonstrated in many studies. Aβ precipitation and toxicity in AD brains seem to be caused by abnormal interactions with neocortical metal ions, especially iron, copper, zinc, and aluminum [1–3]. There is increasing evidence that iron and aluminum ions are involved in the mechanisms that underlie the neurodegenerative diseases [4,5]. However, evidence was brought to demonstrate that some Aβ fragments, at physiological pH, are not able to form binary complexes with Fe(III) ions of sufficient stability to compete with metal hydroxide precipitation [6]. On the contrary, multiple metal ions are known to interact with Aβ peptides [7]. Consequently, we investigated here the interaction of Fe(II/III) and Al(III) ions with some amyloid-β peptides and fragments that results in peptide aggregation and fibrillation [8,9]. Infrared spectroscopy, atomic force microscopy, scanning electron microscopy, electrophoresis and mass spectrometry demonstrated conformational changes of peptides in the presence of such metals.

  12. Draft Genome Sequences for Clostridium thermocellum Wild-Type Strain YS and Derived Cellulose Adhesion-Defective Mutant Strain AD2

    SciTech Connect (OSTI)

    Brown, Steven D; Lamed, Raphael; Morag, Ely; Borovok, Ilya; Shoham, Yuval; Klingeman, Dawn Marie; Johnson, Courtney M; Yang, Zamin; Land, Miriam L; Utturkar, Sagar M; Keller, Martin; Bayer, Edward A

    2012-01-01

    Clostridium thermocellum wild-type strain YS is an anaerobic, thermophilic, cellulolytic bacterium capable of directly converting cellulosic substrates into ethanol. Strain YS and a derived cellulose adhesion-defective mutant strain AD2 played pivotal roles in describing the original cellulosome concept. We present their draft genome sequences.

  13. Therapeutic target for protozoal diseases

    DOE Patents [OSTI]

    Rathore, Dharmendar; Jani, Dewal; Nagarkatti, Rana

    2008-10-21

    A novel Fasciclin Related Adhesive Protein (FRAP) from Plasmodium and related parasites is provided as a target for therapeutic intervention in diseases caused by the parasites. FRAP has been shown to play a critical role in adhesion to, or invasion into, host cells by the parasite. Furthermore, FRAP catalyzes the neutralization of heme by the parasite, by promoting its polymerization into hemozoin. This invention provides methods and compositions for therapies based on the administration of protein, DNA or cell-based vaccines and/or antibodies based on FRAP, or antigenic epitopes of FRAP, either alone or in combination with other parasite antigens. Methods for the development of compounds that inhibit the catalytic activity of FRAP, and diagnostic and laboratory methods utilizing FRAP are also provided.

  14. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Alzheimer's disease, macular degeneration of the retina in diabetes, and mitochondrial diseases in children. In addition, specific processes like protein glycation can also be...

  15. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    these projects will target Alzheimer's disease, macular degeneration of the retina in diabetes, and mitochondrial diseases in children. In addition, specific processes like protein...

  16. Protein Instability and Lou Gehrig's Disease

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Protein Instability and Lou Gehrig's Disease Print A new study links protein instability with the progression of a lethal degenerative disease: amyotrophic lateral sclerosis (ALS), also known as Lou Gehrig's disease. Using several biophysical techniques as well as small-angle x-ray scattering (SAXS) at the Advanced Light Source, researchers focused on the effects of mutations to a gene coding for a protein called superoxide dismutase (SOD). The study provides evidence that those proteins linked

  17. Protein Instability and Lou Gehrig's Disease

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Protein Instability and Lou Gehrig's Disease Print A new study links protein instability with the progression of a lethal degenerative disease: amyotrophic lateral sclerosis (ALS), also known as Lou Gehrig's disease. Using several biophysical techniques as well as small-angle x-ray scattering (SAXS) at the Advanced Light Source, researchers focused on the effects of mutations to a gene coding for a protein called superoxide dismutase (SOD). The study provides evidence that those proteins linked

  18. Protein Instability and Lou Gehrig's Disease

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Protein Instability and Lou Gehrig's Disease Print A new study links protein instability with the progression of a lethal degenerative disease: amyotrophic lateral sclerosis (ALS), also known as Lou Gehrig's disease. Using several biophysical techniques as well as small-angle x-ray scattering (SAXS) at the Advanced Light Source, researchers focused on the effects of mutations to a gene coding for a protein called superoxide dismutase (SOD). The study provides evidence that those proteins linked

  19. Protein Instability and Lou Gehrig's Disease

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Protein Instability and Lou Gehrig's Disease Print A new study links protein instability with the progression of a lethal degenerative disease: amyotrophic lateral sclerosis (ALS), also known as Lou Gehrig's disease. Using several biophysical techniques as well as small-angle x-ray scattering (SAXS) at the Advanced Light Source, researchers focused on the effects of mutations to a gene coding for a protein called superoxide dismutase (SOD). The study provides evidence that those proteins linked

  20. OSTIblog Articles in the ADNI Topic | OSTI, US Dept of Energy Office of

    Office of Scientific and Technical Information (OSTI)

    Scientific and Technical Information ADNI Topic Sharing Data Leads to Progress by Nena Moss 19 Aug, 2010 in Personal Perspectives My mother died in March 2010 after a 15-year battle with Alzheimer's, so I pay particular attention to news about this dreadful disease. A recent New York Times article caught my eye: "Sharing of Data Leads to Progress on Alzheimer's." How did sharing data lead to progress on Alzheimer's? A collaborative effort, the Alzheimer's Disease Neuroimaging

  1. OSTIblog Articles in the biomarkers Topic | OSTI, US Dept of Energy Office

    Office of Scientific and Technical Information (OSTI)

    of Scientific and Technical Information biomarkers Topic Sharing Data Leads to Progress by Nena Moss 19 Aug, 2010 in Personal Perspectives My mother died in March 2010 after a 15-year battle with Alzheimer's, so I pay particular attention to news about this dreadful disease. A recent New York Times article caught my eye: "Sharing of Data Leads to Progress on Alzheimer's." How did sharing data lead to progress on Alzheimer's? A collaborative effort, the Alzheimer's Disease

  2. OSTIblog Articles in the corollary Topic | OSTI, US Dept of Energy Office

    Office of Scientific and Technical Information (OSTI)

    of Scientific and Technical Information corollary Topic Sharing Data Leads to Progress by Nena Moss 19 Aug, 2010 in Personal Perspectives My mother died in March 2010 after a 15-year battle with Alzheimer's, so I pay particular attention to news about this dreadful disease. A recent New York Times article caught my eye: "Sharing of Data Leads to Progress on Alzheimer's." How did sharing data lead to progress on Alzheimer's? A collaborative effort, the Alzheimer's Disease

  3. Imaging of occupational and environmental lung diseases

    SciTech Connect (OSTI)

    Akira, M.

    2008-03-15

    The chest radiograph is the basic tool for identifying occupational and environmental lung diseases; however, its sensitivity and specificity for the diagnosis of occupational and environmental lung diseases are low. High-resolution CT is the optimal method of recognizing parenchymal abnormalities in occupational and environmental disease. With the exception of pleural plaques, the CT findings of occupational and environmental lung diseases are nonspecific. Therefore, correlation of imaging features with history of exposure, other clinical features, and sometimes pathology is needed for the diagnosis of pneumoconiosis.

  4. Protein Instability and Lou Gehrig's Disease

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    called superoxide dismutase (SOD). The study provides evidence that those proteins linked to more severe forms of the disease are less stable structurally and more prone to...

  5. A Security Solution for IEEE 802.11's Ad-hoc Mode:Password-Authentication and Group Diffie-Hellman Key Exchange

    SciTech Connect (OSTI)

    Emmanuel, Bresson; Olivier, Chevassut; David, Pointcheval

    2005-10-01

    The IEEE 802 standards ease the deployment of networkinginfrastructures and enable employers to accesscorporate networks whiletraveling. These standards provide two modes of communication calledinfrastructure and ad-hoc modes. A security solution for the IEEE802.11's infrastructure mode took several years to reach maturity andfirmware are still been upgraded, yet a solution for the ad-hoc modeneeds to be specified. The present paper is a first attempt in thisdirection. It leverages the latest developments in the area ofpassword-based authentication and (group) Diffie-Hellman key exchange todevelop a provably-secure key-exchange protocol for IEEE 802.11's ad-hocmode. The protocol allows users to securely join and leave the wirelessgroup at time, accommodates either a single-shared password orpairwise-shared passwords among the group members, or at least with acentral server; achieves security against dictionary attacks in theideal-hash model (i.e. random-oracles). This is, to the best of ourknowledge, the first such protocol to appear in the cryptographicliterature.

  6. Disease Ontology 2015 update: An expanded and updated database of human diseases for linking biomedical knowledge through disease data

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Kibbe, Warren A.; Arze, Cesar; Felix, Victor; Mitraka, Elvira; Bolton, Evan; Fu, Gang; Mungall, Christopher J.; Binder, Janos X.; Malone, James; Vasant, Drashtti; et al

    2014-10-27

    The current version of the Human Disease Ontology (DO) (http://www.disease-ontology.org) database expands the utility of the ontology for the examination and comparison of genetic variation, phenotype, protein, drug and epitope data through the lens of human disease. DO is a biomedical resource of standardized common and rare disease concepts with stable identifiers organized by disease etiology. The content of DO has had 192 revisions since 2012, including the addition of 760 terms. Thirty-two percent of all terms now include definitions. DO has expanded the number and diversity of research communities and community members by 50+ during the past two years.more » These community members actively submit term requests, coordinate biomedical resource disease representation and provide expert curation guidance. Since the DO 2012 NAR paper, there have been hundreds of term requests and a steady increase in the number of DO listserv members, twitter followers and DO website usage. DO is moving to a multi-editor model utilizing Protégé to curate DO in web ontology language. In conclusion, this will enable closer collaboration with the Human Phenotype Ontology, EBI's Ontology Working Group, Mouse Genome Informatics and the Monarch Initiative among others, and enhance DO's current asserted view and multiple inferred views through reasoning.« less

  7. A Principal Component Analysis Noise Filter Value-Added Procedure to Remove Uncorrelated Noise from Atmospheric Emitted Radiance Interferometer (AERI) Observations

    SciTech Connect (OSTI)

    C. Lo D. D. Turner R. O. Knuteson

    2006-01-31

    This technical report provide a short description of the application of the principle component analysis techniques to remove uncorrelated random noise from ground-based high spectral resolution infrared radiance observations collected by the atmospheric emitted radiance interferometers (AERIs) deployed by the Atmospheric Radiation Measurement (ARM) Program. A general overview of the technique, the input, and output datastreams of the newly generated value-added product, and the data quality checks used are provided. A more complete discussion of the theory and results is given in Turner et al. (2006).

  8. Protein Instability and Lou Gehrig's Disease

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1941 at the age of only 38. Recently, an award-winning movie about the famous physicist, Stephen Hawking, depicted the disease's devastating physical toll. ALS kills by destroying...

  9. Zika Virus Disease and Prevention Presentation

    Broader source: Energy.gov [DOE]

    A “Zika Virus Disease and Prevention” presentation was made by Bonnie S. Richter, MPH, PhD, at the May 19 Office of Environment, Health, Safety and Security (EHSS) All Hands Meeting.

  10. Metabolomics reveals metabolic biomarkers of Crohn's disease

    SciTech Connect (OSTI)

    Jansson, J.K.; Willing, B.; Lucio, M.; Fekete, A.; Dicksved, J.; Halfvarson, J.; Tysk, C.; Schmitt-Kopplin, P.

    2009-06-01

    The causes and etiology of Crohn's disease (CD) are currently unknown although both host genetics and environmental factors play a role. Here we used non-targeted metabolic profiling to determine the contribution of metabolites produced by the gut microbiota towards disease status of the host. Ion Cyclotron Resonance Fourier Transform Mass Spectrometry (ICR-FT/MS) was used to discern the masses of thousands of metabolites in fecal samples collected from 17 identical twin pairs, including healthy individuals and those with CD. Pathways with differentiating metabolites included those involved in the metabolism and or synthesis of amino acids, fatty acids, bile acids and arachidonic acid. Several metabolites were positively or negatively correlated to the disease phenotype and to specific microbes previously characterized in the same samples. Our data reveal novel differentiating metabolites for CD that may provide diagnostic biomarkers and/or monitoring tools as well as insight into potential targets for disease therapy and prevention.

  11. Counting small RNA in disease-causing organisms

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in disease-causing organisms Los Alamos researchers demonstrated improved technical methods capable of directly counting small RNA molecules in pathogenic (disease-causing)...

  12. Transgenic Mouse Model of Chronic Beryllium Disease

    SciTech Connect (OSTI)

    Gordon, Terry

    2009-05-26

    Animal models provide powerful tools for dissecting dose-response relationships and pathogenic mechanisms and for testing new treatment paradigms. Mechanistic research on beryllium exposure-disease relationships is severely limited by a general inability to develop a sufficient chronic beryllium disease animal model. Discovery of the Human Leukocyte Antigen (HLA) - DPB1Glu69 genetic susceptibility component of chronic beryllium disease permitted the addition of this human beryllium antigen presentation molecule to an animal genome which may permit development of a better animal model for chronic beryllium disease. Using FVB/N inbred mice, Drs. Rubin and Zhu, successfully produced three strains of HLA-DPB1 Glu 69 transgenic mice. Each mouse strain contains a haplotype of the HLA-DPB1 Glu 69 gene that confers a different magnitude of odds ratio (OR) of risk for chronic beryllium disease: HLA-DPB1*0401 (OR = 0.2), HLA-DPB1*0201 (OR = 15), HLA-DPB1*1701 (OR = 240). In addition, Drs. Rubin and Zhu developed transgenic mice with the human CD4 gene to permit better transmission of signals between T cells and antigen presenting cells. This project has maintained the colonies of these transgenic mice and tested the functionality of the human transgenes.

  13. Chronic beryllium disease: Diagnosis and management

    SciTech Connect (OSTI)

    Rossman, M.D.

    1996-10-01

    Chronic beryllium disease is predominantly a pulmonary granulomatosis that was originally described in 1946. Symptoms usually include dyspnea and cough. Fever, anorexia, and weight loss are common. Skin lesions are the most common extrathoracic manifestation. Granulomatous hepatitis, hypercalcemia, and kidney stones can also occur. Radiographic and physiologic abnormalities are similar to those in sarcoidosis. While traditionally the pathologic changes included granulomas and cellular interstitial changes, the hallmark of the disease today is the well-formed granuloma. Immunologic studies have demonstrated a cell-mediated response to beryllium that is due to an accumulation of CD4{sup +} T cells at the site of disease activity. Diagnosis depends on the demonstration of pathologic changes (i.e., granuloma) and evidence that the granuloma was caused by a hypersensitivity to beryllium (i.e., positive lung proliferative response to beryllium). Using these criteria, the diagnosis of chronic beryllium disease can now be made before the onset of clinical symptoms. Whether, with early diagnosis, the natural course of this condition will be the same as when it was traditionally diagnosed is not known. Currently, corticosteroids are used to treat patients with significant symptoms or evidence of progressive disease. 21 refs.

  14. Global disease monitoring and forecasting with Wikipedia

    SciTech Connect (OSTI)

    Generous, Nicholas; Fairchild, Geoffrey; Deshpande, Alina; Del Valle, Sara Y.; Priedhorsky, Reid; Salathé, Marcel

    2014-11-13

    Infectious disease is a leading threat to public health, economic stability, and other key social structures. Efforts to mitigate these impacts depend on accurate and timely monitoring to measure the risk and progress of disease. Traditional, biologically-focused monitoring techniques are accurate but costly and slow; in response, new techniques based on social internet data, such as social media and search queries, are emerging. These efforts are promising, but important challenges in the areas of scientific peer review, breadth of diseases and countries, and forecasting hamper their operational usefulness. We examine a freely available, open data source for this use: access logs from the online encyclopedia Wikipedia. Using linear models, language as a proxy for location, and a systematic yet simple article selection procedure, we tested 14 location-disease combinations and demonstrate that these data feasibly support an approach that overcomes these challenges. Specifically, our proof-of-concept yields models with up to 0.92, forecasting value up to the 28 days tested, and several pairs of models similar enough to suggest that transferring models from one location to another without re-training is feasible. Based on these preliminary results, we close with a research agenda designed to overcome these challenges and produce a disease monitoring and forecasting system that is significantly more effective, robust, and globally comprehensive than the current state of the art.

  15. Global disease monitoring and forecasting with Wikipedia

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Generous, Nicholas; Fairchild, Geoffrey; Deshpande, Alina; Del Valle, Sara Y.; Priedhorsky, Reid; Salathé, Marcel

    2014-11-13

    Infectious disease is a leading threat to public health, economic stability, and other key social structures. Efforts to mitigate these impacts depend on accurate and timely monitoring to measure the risk and progress of disease. Traditional, biologically-focused monitoring techniques are accurate but costly and slow; in response, new techniques based on social internet data, such as social media and search queries, are emerging. These efforts are promising, but important challenges in the areas of scientific peer review, breadth of diseases and countries, and forecasting hamper their operational usefulness. We examine a freely available, open data source for this use: accessmore » logs from the online encyclopedia Wikipedia. Using linear models, language as a proxy for location, and a systematic yet simple article selection procedure, we tested 14 location-disease combinations and demonstrate that these data feasibly support an approach that overcomes these challenges. Specifically, our proof-of-concept yields models with up to 0.92, forecasting value up to the 28 days tested, and several pairs of models similar enough to suggest that transferring models from one location to another without re-training is feasible. Based on these preliminary results, we close with a research agenda designed to overcome these challenges and produce a disease monitoring and forecasting system that is significantly more effective, robust, and globally comprehensive than the current state of the art.« less

  16. Cholesterol accumulation in Niemann Pick type C (NPC) model cells causes a shift in APP localization to lipid rafts

    SciTech Connect (OSTI)

    Kosicek, Marko; Malnar, Martina; Goate, Alison; Hecimovic, Silva

    2010-03-12

    It has been suggested that cholesterol may modulate amyloid-{beta} (A{beta}) formation, a causative factor of Alzheimer's disease (AD), by regulating distribution of the three key proteins in the pathogenesis of AD ({beta}-amyloid precursor protein (APP), {beta}-secretase (BACE1) and/or presenilin 1 (PS1)) within lipid rafts. In this work we tested whether cholesterol accumulation upon NPC1 dysfunction, which causes Niemann Pick type C disease (NPC), causes increased partitioning of APP into lipid rafts leading to increased CTF/A{beta} formation in these cholesterol-rich membrane microdomains. To test this we used CHO NPC1{sup -/-} cells (NPC cells) and parental CHOwt cells. By sucrose density gradient centrifugation we observed a shift in fl-APP/CTF compartmentalization into lipid raft fractions upon cholesterol accumulation in NPC vs. wt cells. Furthermore, {gamma}-secretase inhibitor treatment significantly increased fl-APP/CTF distribution in raft fractions in NPC vs. wt cells, suggesting that upon cholesterol accumulation in NPC1-null cells increased formation of APP-CTF and its increased processing towards A{beta} occurs in lipid rafts. Our results support that cholesterol overload, such as in NPC disease, leads to increased partitioning of APP/CTF into lipid rafts resulting in increased amyloidogenic processing of APP in these cholesterol-rich membranes. This work adds to the mechanism of the cholesterol-effect on APP processing and the pathogenesis of Alzheimer's disease and supports the role of lipid rafts in these processes.

  17. Ad Building demolition, recycling completed

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ARSEC Environmental, LLC was the general contractor for the demolition of the structure-four stories plus a basement-which opened in 1956 and closed in September 2008. Norris ...

  18. Adding Students and Multiplying Scientists

    Office of Energy Efficiency and Renewable Energy (EERE)

    This month, students across the country will begin competing in regional contests for the chance to attend the National Science Bowl in Washington, D.C.

  19. Treatment of Anthrax Disease Frequently Asked Questions

    SciTech Connect (OSTI)

    Judd, Kathleen S.; Young, Joan E.; Lesperance, Ann M.; Malone, John D.

    2010-05-14

    This document provides a summary of Frequently Asked Questions (FAQs) on the treatment of anthrax disease caused by a wide-area release of Bacillus anthracis spores as an act bioterrorism. These FAQs are intended to provide the public health and medical community, as well as others, with guidance and communications to support the response and long-term recovery from an anthrax event.

  20. Stanford Synchrotron Radiation Lightsource

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    melanoma, and degenerative diseases like multiple sclerosis, Alzheimer's and Type 2 diabetes. Understanding of how Wnt proteins bind and activate Frizzled receptors is important...

  1. Accident Investigation of the June 1, 2013 Stairway Fall Resulting...

    Broader source: Energy.gov (indexed) [DOE]

    show that examinations were conducted for employees with various illnesses, such as diabetes and Alzheimer's disease, which occurred off the job 9 . The Board believes that if a...

  2. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    groups are not removed in needed situations) is associated with multiple human cancers, Alzheimer's Disease, and increased susceptibility to pathogen infections. To shed light on...

  3. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    view of how promising drugs act within specific cells. Some of these projects will target Alzheimer's disease, macular degeneration of the retina in diabetes, and mitochondrial...

  4. New Crystal Structures Lift Fog around Protein Folding

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    incorrect or "misfolding" of proteins has been linked to many diseases, including Alzheimer's, Parkinson's, and some forms of cancer. So far, however, a complete...

  5. Combined state-adding and state-deleting approaches to type III multi-step rationally extended potentials: Applications to ladder operators and superintegrability

    SciTech Connect (OSTI)

    Marquette, Ian; Quesne, Christiane

    2014-11-15

    Type III multi-step rationally extended harmonic oscillator and radial harmonic oscillator potentials, characterized by a set of k integers m{sub 1}, m{sub 2}, ⋯, m{sub k}, such that m{sub 1} < m{sub 2} < ⋯ < m{sub k} with m{sub i} even (resp. odd) for i odd (resp. even), are considered. The state-adding and state-deleting approaches to these potentials in a supersymmetric quantum mechanical framework are combined to construct new ladder operators. The eigenstates of the Hamiltonians are shown to separate into m{sub k} + 1 infinite-dimensional unitary irreducible representations of the corresponding polynomial Heisenberg algebras. These ladder operators are then used to build a higher-order integral of motion for seven new infinite families of superintegrable two-dimensional systems separable in cartesian coordinates. The finite-dimensional unitary irreducible representations of the polynomial algebras of such systems are directly determined from the ladder operator action on the constituent one-dimensional Hamiltonian eigenstates and provide an algebraic derivation of the superintegrable systems whole spectrum including the level total degeneracies.

  6. YALINA facility a sub-critical Accelerator- Driven System (ADS) for nuclear energy research facility description and an overview of the research program (1997-2008).

    SciTech Connect (OSTI)

    Gohar, Y.; Smith, D. L.; Nuclear Engineering Division

    2010-04-28

    The YALINA facility is a zero-power, sub-critical assembly driven by a conventional neutron generator. It was conceived, constructed, and put into operation at the Radiation Physics and Chemistry Problems Institute of the National Academy of Sciences of Belarus located in Minsk-Sosny, Belarus. This facility was conceived for the purpose of investigating the static and dynamic neutronics properties of accelerator driven sub-critical systems, and to serve as a neutron source for investigating the properties of nuclear reactions, in particular transmutation reactions involving minor-actinide nuclei. This report provides a detailed description of this facility and documents the progress of research carried out there during a period of approximately a decade since the facility was conceived and built until the end of 2008. During its history of development and operation to date (1997-2008), the YALINA facility has hosted several foreign groups that worked with the resident staff as collaborators. The participation of Argonne National Laboratory in the YALINA research programs commenced in 2005. For obvious reasons, special emphasis is placed in this report on the work at YALINA facility that has involved Argonne's participation. Attention is given here to the experimental program at YALINA facility as well as to analytical investigations aimed at validating codes and computational procedures and at providing a better understanding of the physics and operational behavior of the YALINA facility in particular, and ADS systems in general, during the period 1997-2008.

  7. Adding flexibility to the particles-on-a-sphere model for large-amplitude motion: POSflex force field for protonated methane

    SciTech Connect (OSTI)

    Uhl, Felix; Walewski, ?ukasz; Forbert, Harald; Marx, Dominik

    2014-09-14

    The so-called particles-on-a-sphere (POS) model has been introduced a while ago in order to describe in simple terms large-amplitude motion of polyatomic hydrides, XH{sub n}. The POS model of protonated methane, CH{sub 5}{sup +}, has been shown to capture well the essence of the fluxional nature of this enigmatic floppy molecule. Here, we extend this model to the POSflex force field by adding flexibility to the CH bonds, which are constrained to a common fixed bond length in the original model. This makes the present model extremely efficient for computer simulation, including path integral molecular dynamics in order to assess the crucial quantum effects on nuclear motion at low temperatures. Moreover, the POSflex force field can be conveniently used to study microsolvation effects upon combining it with intermolecular pair potentials to account for solute-solvent interactions. Upon computing static properties as well as thermal and quantum fluctuation effects at ambient and low temperatures, respectively, it is shown that the POSflex model is very well suited to describe the structural properties of bare CH{sub 5}{sup +}, including hydrogen scrambling and thus fluxionality in the first place. The far- to mid-infrared spectrum up to the bending band is roughly described, whereas the model fails to account for the well-structured stretching band by construction.

  8. Logarithmic correction in the deformed AdS{sub 5} model to produce the heavy quark potential and QCD beta function

    SciTech Connect (OSTI)

    He Song; Huang Mei; Yan Qishu

    2011-02-15

    We study the holographic QCD model, which contains a quadratic term -{sigma}z{sup 2} and a logarithmic term -c{sub 0}log[(z{sub IR}-z)/z{sub IR}] with an explicit infrared cutoff z{sub IR} in the deformed AdS{sub 5} warp factor. We investigate the heavy-quark potential for three cases, i.e., with only a quadratic correction, with both quadratic and logarithmic corrections, and with only a logarithmic correction. We solve the dilaton field and dilation potential from the Einstein equation and investigate the corresponding beta function in the Guersoy-Kiritsis-Nitti framework. Our studies show that in the case with only a quadratic correction, a negative {sigma} or the Andreev-Zakharov model is favored to fit the heavy-quark potential and to produce the QCD beta function at 2-loop level; however, the dilaton potential is unbounded in the infrared regime. One interesting observation for the case of positive {sigma} is that the corresponding beta function exists in an infrared fixed point. In the case with only a logarithmic correction, the heavy-quark Cornell potential can be fitted very well, the corresponding beta function agrees with the QCD beta function at 2-loop level reasonably well, and the dilaton potential is bounded from below in the infrared. At the end, we propose a more compact model which has only a logarithmic correction in the deformed warp factor and has less free parameters.

  9. Perfect fluidity of a dissipative system: Analytical solution for the Boltzmann equation in AdS2 Ⓧ S2

    SciTech Connect (OSTI)

    Noronha, Jorge; Denicol, Gabriel S.

    2015-12-30

    In this paper we obtain an analytical solution of the relativistic Boltzmann equation under the relaxation time approximation that describes the out-of-equilibrium dynamics of a radially expanding massless gas. This solution is found by mapping this expanding system in flat spacetime to a static flow in the curved spacetime AdS2 Ⓧ S2. We further derive explicit analytic expressions for the momentum dependence of the single-particle distribution function as well as for the spatial dependence of its moments. We find that this dissipative system has the ability to flow as a perfect fluid even though its entropy density does not match the equilibrium form. The nonequilibrium contribution to the entropy density is shown to be due to higher-order scalar moments (which possess no hydrodynamical interpretation) of the Boltzmann equation that can remain out of equilibrium but do not couple to the energy-momentum tensor of the system. Furthermore, in this system the slowly moving hydrodynamic degrees of freedom can exhibit true perfect fluidity while being totally decoupled from the fast moving, nonhydrodynamical microscopic degrees of freedom that lead to entropy production.

  10. Science On Tap - Understanding HIV and Infectious Diseases

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Science On Tap - Understanding HIV and Infectious Diseases Science On Tap - Understanding HIV and Infectious Diseases WHEN: Sep 17, 2015 5:30 PM - 7:00 PM WHERE: UnQuarked Wine...

  11. Software speeds detection of diseases and cancer-treatment targets

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Software speeds detection of diseases Software speeds detection of diseases and cancer-treatment targets The Lab has released an updated version of software that is now capable of ...

  12. NIOSH Alert-Preventing Sentization and Disease from Beryllium | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy NIOSH Alert-Preventing Sentization and Disease from Beryllium NIOSH Alert-Preventing Sentization and Disease from Beryllium February 3, 2011 Preventing Sensitization and Disease from Beryllium Exposure The National Institute for Occupational Safety and Health (NIOSH) requests assistance in preventing beryllium sensitization and chronic beryllium disease. Development of these conditions requires exposure to beryllium and is affected by both job tasks and genetic factors. Some jobs

  13. Presentation, Zika Virus Disease and Prevention | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Presentation, Zika Virus Disease and Prevention Presentation, Zika Virus Disease and Prevention May 19, 2016 Dr. Richter presented information about the rapid evolution of the Zika virus, the regions reporting active mosquito transmission of the Zika virus, the disease symptoms and preventative steps. Presentation, Zika Virus Disease and Prevention (678.7 KB) More Documents & Publications Worker Health Summary, 1995-2004 CAIRS Direct Data Entry (CDDE) Online Training Package FAQS Reference

  14. A customizable model for chronic disease coordination: Lessons learned from the coordinated chronic disease program

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Voetsch, Karen; Sequeira, Sonia; Chavez, Amy Holmes

    2016-03-31

    In 2012, the Centers for Disease Control and Prevention provided funding and technical assistance to all states and territories to implement the Coordinated Chronic Disease Program, marking the first time that all state health departments had federal resources to coordinate chronic disease prevention and control programs. This article describes lessons learned from this initiative and identifies key elements of a coordinated approach. We analyzed 80 programmatic documents from 21 states and conducted semistructured interviews with 7 chronic disease directors. Six overarching themes emerged: 1) focused agenda, 2) identification of functions, 3) comprehensive planning, 4) collaborative leadership and expertise, 5) managedmore » resources, and 6) relationship building. Furthermore, these elements supported 4 essential activities: 1) evidence-based interventions, 2) strategic use of staff, 3) consistent communication, and 4) strong program infrastructure. On the basis of these elements and activities, we propose a conceptual model that frames overarching concepts, skills, and strategies needed to coordinate state chronic disease prevention and control programs.« less

  15. Evaluation of the deposition, translocation and pathological response of brake dust with and without added chrysotile in comparison to crocidolite asbestos following short-term inhalation: Interim results

    SciTech Connect (OSTI)

    Bernstein, David M.; Rogers, Rick; Sepulveda, Rosalina; Kunzendorf, Peter; Bellmann, Bernd; Ernst, Heinrich; Phillips, James I.

    2014-04-01

    Chrysotile has been frequently used in the past in manufacturing brakes and continues to be used in brakes in many countries. This study was designed to provide an understanding of the biokinetics and potential toxicology following inhalation of brake dust following short term exposure in rats. The deposition, translocation and pathological response of brake dust derived from brake pads manufactured with chrysotile were evaluated in comparison to the amphibole, crocidolite asbestos. Rats were exposed by inhalation 6 h/day for 5 days to either brake dust obtained by sanding of brake-drums manufactured with chrysotile, a mixture of chrysotile and the brake dust or crocidolite asbestos. No significant pathological response was observed at any time point in either the brake dust or chrysotile/brake dust exposure groups. The long chrysotile fibers (> 20 μm) cleared quickly with T{sub 1/2} estimated as 30 and 33 days, respectively in the brake dust and the chrysotile/brake dust exposure groups. In contrast, the long crocidolite fibers had a T{sub 1/2} > 1000 days and initiated a rapid inflammatory response in the lung following exposure resulting in a 5-fold increase in fibrotic response within 91 days. These results provide support that brake dust derived from chrysotile containing brake drums would not initiate a pathological response in the lung following short term inhalation. - Highlights: • We evaluated brake dust w/wo added chrysotile in comparison to crocidolite asbestos. • Persistence, translocation, pathological response in the lung and pleural cavity. • Chrysotile cleared rapidly from the lung while the crocidolite asbestos persisted. • No significant pathology observed at any time point in the brake-dust groups. • Crocidolite produced pathological response - Wagner 4 interstitial fibrosis by 32d.

  16. Visualizing Brain Metals in Health and Disease

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Visualizing Brain Metals in Health and Disease figure 1 Fig. 1. Rapid-scanning x-ray fluorescence mapping ex perimental setup. Synchrotron x-rays at 11 keV passed through a 50 µm aperture (Ap). The beam intensity was monitored with a N2-filled ion chamber (I0). The brain slice was mounted vertically on a motorized stage (St) at 45° to the incident x-ray beam and raster scanned in the beam. A 13-element Ge detector (Ge) was positioned at a 90° angle to the beam. We all require iron, copper and

  17. Analyzing and modeling the kinetics of amyloid beta pores associated with Alzheimer’s disease pathology

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ullah, Ghanim; Demuro, Angelo; Parker, Ian; Pearson, John E.; Xu, Shang -Zhong

    2015-09-08

    Amyloid beta (Aβ) oligomers associated with Alzheimer’s disease (AD) form Ca2+-permeable plasma membrane pores, leading to a disruption of the otherwise well-controlled intracellular calcium (Ca2+) homeostasis. The resultant up-regulation of intracellular Ca2+ concentration has detrimental implications for memory formation and cell survival. The gating kinetics and Ca2+ permeability of Aβ pores are not well understood. We have used computational modeling in conjunction with the ability of optical patch-clamping for massively parallel imaging of Ca2+ flux through thousands of pores in the cell membrane of Xenopus oocytes to elucidate the kinetic properties of Aβ pores. The fluorescence time-series data from individualmore » pores were idealized and used to develop data-driven Markov chain models for the kinetics of the Aβ pore at different stages of its evolution. Our study provides the first demonstration of developing Markov chain models for ion channel gating that are driven by optical-patch clamp data with the advantage of experiments being performed under close to physiological conditions. As a result, we demonstrate the up-regulation of gating of various Ca2+ release channels due to Aβ pores and show that the extent and spatial range of such up-regulation increases as Aβ pores with low open probability and Ca2+ permeability transition into those with high open probability and Ca2+ permeability.« less

  18. Chronic Beryllium Disease Prevention Program Report

    SciTech Connect (OSTI)

    Lee, S

    2012-03-29

    This document describes how Lawrence Livermore National Laboratory (LLNL) meets the requirements and management practices of federal regulation 10 CFR 850, 'Chronic Beryllium Disease Prevention Program (CBDPP).' This revision of the LLNL CBDPP incorporates clarification and editorial changes based on lessons learned from employee discussions, observations and reviews of Department of Energy (DOE) Complex and commercial industry beryllium (Be) safety programs. The information is used to strengthen beryllium safety practices at LLNL, particularly in the areas of: (1) Management of small parts and components; and (2) Communication of program status to employees. Future changes to LLNL beryllium activities and on-going operating experience will be incorporated into the program as described in Section S, 'Performance Feedback.'

  19. Respiratory disease in Utah coal miners

    SciTech Connect (OSTI)

    Rom, W.N.; Kanner, R.E.; Renzetti, A.D. Jr.; Shigeoka, J.W.; Barkman, H.W.; Nichols, M.; Turner, W.A.; Coleman, M.; Wright, W.E.

    1981-04-01

    Two hundred forty-two Utah underground coal miners volunteered to participate in a respiratory disease study. They were an older group (mean, 56 years of age) and had spent a mean of 29 years in the coal-mining industry. The prevalence of chronic bronchitis was 57%, and that of coal worker's pneumoconiosis, 25%; only one worker had progressive massive fibrosis. Significant impairment of pulmonary function was found among those with a history of cigarette smoking. Chronic bronchitis or coal worker's penumoconiosis among nonsmokers did not impair pulmonary function. There was a significant association among the nonsmokers between increasing exposure to coal dust and coal worker's pneumoconiosis, but not for changes in pulmonary function. Coal mine dust had a significant influence in causing the symptom complex of chronic cough and sputum production, and coal worker's pneumoconiosis.

  20. Respiratory disease in Utah coal miners

    SciTech Connect (OSTI)

    Rom, W.N.; Kanner, R.E.; Renzetti, A.D. Jr.; Shigeoka, J.W.; Barkman, H.W.; Nichols, M.; Turner, W.A.; Coleman, M.; Wright, W.E.

    1981-04-01

    Two hundred forty-two Utah underground coal miners volunteered to participate in a respiratory disease study. They were an older group (mean, 56 years of age) and had spent a mean of 29 years in the coal-mining industry. The prevalence of chronic bronchitis was 57%, and that of coal worker's pneumoconiosis, 25%; only one worker had progressive massive fibrosis. Significant impairment of pulmonary function was found among those with a history of cigarette smoking. Chronic bronchitis or coal worker's pneumoconiosis among nonsmokers did not impair pulmonary function. There was a significant association among the nonsmokers between increasing exposure to coal dust and coal worker's pneumoconiosis, but not for changes in pulmonary function. Coal mine dust had a significant influence in causing the symptom complex of chronic cough and sputum production, and coal worker's pneumoconiosis.

  1. Optical detection dental disease using polarized light

    DOE Patents [OSTI]

    Everett, Matthew J.; Colston, Jr., Billy W.; Sathyam, Ujwal S.; Da Silva, Luiz B.; Fried, Daniel

    2003-01-01

    A polarization sensitive optical imaging system is used to detect changes in polarization in dental tissues to aid the diagnosis of dental disease such as caries. The degree of depolarization is measured by illuminating the dental tissue with polarized light and measuring the polarization state of the backscattered light. The polarization state of this reflected light is analyzed using optical polarimetric imaging techniques. A hand-held fiber optic dental probe is used in vivo to direct the incident beam to the dental tissue and collect the reflected light. To provide depth-resolved characterization of the dental tissue, the polarization diagnostics may be incorporated into optical coherence domain reflectometry and optical coherence tomography (OCDR/OCT) systems, which enables identification of subsurface depolarization sites associated with demineralization of enamel or bone.

  2. Automated diagnostic kiosk for diagnosing diseases

    SciTech Connect (OSTI)

    Regan, John Frederick; Birch, James Michael

    2014-02-11

    An automated and autonomous diagnostic apparatus that is capable of dispensing collection vials and collections kits to users interesting in collecting a biological sample and submitting their collected sample contained within a collection vial into the apparatus for automated diagnostic services. The user communicates with the apparatus through a touch-screen monitor. A user is able to enter personnel information into the apparatus including medical history, insurance information, co-payment, and answer a series of questions regarding their illness, which is used to determine the assay most likely to yield a positive result. Remotely-located physicians can communicate with users of the apparatus using video tele-medicine and request specific assays to be performed. The apparatus archives submitted samples for additional testing. Users may receive their assay results electronically. Users may allow the uploading of their diagnoses into a central databank for disease surveillance purposes.

  3. IMPACT OF SB4 TANK 40 DECANT AND ARP/MCU ADDITIONS WITH/WITHOUT ADDED CAUSTIC ON DWPF CPC PERFORMANCE

    SciTech Connect (OSTI)

    Koopman, D; David Best, D; Frances Williams, F

    2008-04-18

    cases. Relatively insignificant rheological changes were noted other than the previously established thinning from higher acid additions. SRAT and SME processing at 130% acid stoichiometry with and without ARP/MCU additions were very similar. MCU nitric acid content, however, shifted the normal acid addition prior to boiling toward more formic acid and less nitric acid than without MCU. SRNL found no technical issues processing the proposed Tank 40 decant (with or without added NaOH) from the standpoint of the impact on the DWPF SRAT and SME cycles subject to the inherent uncertainties in modeling the ARP noble metal concentrations. The noble metals in the ARP were set to the same weight percents in the total solids that they were measured at in the SB4 waste, which was considered likely to be bounding on the actual noble metal content of the ARP transfer to DWPF. Assumptions for formate loss and nitrite to nitrate conversion can be left at the current levels.

  4. Stanford Geothermal Workshop

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Added

  5. Chronic Beryllium Disease Prevention Program (10 CFR 850) | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Chronic Beryllium Disease Prevention Program (10 CFR 850) Chronic Beryllium Disease Prevention Program (10 CFR 850) The DOE established a chronic beryllium disease prevention program (CBDPP) to reduce the number of workers currently exposed to beryllium in the course of their work at DOE facilities managed by DOE or its contractors, minimize the levels of, and potential for, exposure to beryllium, and establish medical surveillance requirements to ensure early detection of the

  6. Analyzing genetic tree sheds new light on disease outbreaks

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Analyzing genetic tree sheds new light on disease outbreaks Analyzing genetic tree sheds new light on disease outbreaks The team used computational phylogenetic analysis to examine how strains of HIV, both in computer modeling and compared with real-life case studies, would be transmitted. February 25, 2016 Using computational techniques, scientists at Los Alamos National Laboratory are working to more clearly understand how diseases such as HIV are spread. In this image, arrows indicate actual

  7. Recombinant herpes simplex virus useful for treating neoplastic disease

    DOE Patents [OSTI]

    Whitley, Richard J.; Roizman, Bernard

    2010-06-29

    Recombinant herpes simplex viruses comprising DNA encoding cytokines and methods for treating neoplastic diseases using the inventive recombinant viruses are disclosed.

  8. LANL spinoff receives NIH grant for respiratory disease diagnostic...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    LANL spinoff receives NIH grant LANL spinoff receives NIH grant for respiratory disease diagnostic device Mesa Tech has been awarded a grant to develop an inexpensive, ...

  9. Indoor air quality & airborne disease control in healthcare facilities...

    Office of Scientific and Technical Information (OSTI)

    Subject: 32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION; MEDICAL ESTABLISHMENTS; INDOOR AIR POLLUTION; CONTROL SYSTEMS; DISEASES; THERMAL COMFORT; SPACE HVAC SYSTEMS Word ...

  10. AND NUCLEAR MEDICINE; DIAGNOSIS; DISEASES; GAMMA CAMERAS; GENETICS...

    Office of Scientific and Technical Information (OSTI)

    Converting energy to medical progress nuclear medicine NONE 62 RADIOLOGY AND NUCLEAR MEDICINE; DIAGNOSIS; DISEASES; GAMMA CAMERAS; GENETICS; NUCLEAR MEDICINE; PATIENTS; RADIATION...

  11. Update on the Hanford Site Chronic Beryllium Disease Prevention...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Chronic Beryllium Disease Prevention Program (CBDPP) Stan Branch Richland Operations Office Hanford Advisory Board Health, Safety and Environmental Protection Committee January 7,...

  12. Methods of treating parkinson's disease using viral vectors

    DOE Patents [OSTI]

    Bankiewicz, Krys; Cunningham, Janet

    2009-05-19

    Methods of delivering viral vectors, particularly recombinant AAV virions, to the CNS are provided. Also provided are methods of treating Parkinson's Disease.

  13. Structure of the Newcastle disease virus hemagglutinin-neuraminidase...

    Office of Scientific and Technical Information (OSTI)

    virus hemagglutinin-neuraminidase (HN) ectodomain reveals a four-helix bundle stalk Citation Details In-Document Search Title: Structure of the Newcastle disease virus ...

  14. Impact of Cranial Irradiation Added to Intrathecal Conditioning in Hematopoietic Cell Transplantation in Adult Acute Myeloid Leukemia With Central Nervous System Involvement

    SciTech Connect (OSTI)

    Mayadev, Jyoti S.; Douglas, James G.; Storer, Barry E.; Appelbaum, Frederick R.; Storb, Rainer

    2011-05-01

    Purpose: Neither the prognostic importance nor the appropriate management of central nervous system (CNS) involvement is known for patients with acute myeloid leukemia (AML) undergoing hematopoietic cell transplantation (HCT). We examined the impact of a CNS irradiation boost to standard intrathecal chemotherapy (ITC). Methods and Materials: From 1995 to 2005, a total of 648 adult AML patients received a myeloablative HCT: 577 patients were CNS negative (CNS-), and 71 were CNS positive (CNS+). Of the 71 CNS+ patients, 52 received intrathecal chemotherapy alone (CNS+ITC), and 19 received ITC plus an irradiation boost (CNS+RT). Results: The CNS-, CNS+ITC, and CNS+RT patients had 1- and 5-year relapse-free survivals (RFS) of 43% and 35%, 15% and 6%, and 37% and 32%, respectively. CNS+ITC patients had a statistically significant worse RFS compared with CNS- patients (hazard ratio [HR], 2.65; 95% confidence interval [CI], 2.0-3.6; p < 0.0001). CNS+RT patients had improved relapse free survival over that of CNS+ITC patients (HR, 0.45; 95% CI, 0.2-0.8; p = 0.01). The 1- and 5-year overall survivals (OS) of patients with CNS-, CNS+ITC, and CNS+RT, were 50% and 38%, 21% and 6%, and 53% and 42%, respectively. The survival of CNS+RT were significantly better than CNS+ITC patients (p = 0.004). After adjusting for known risk factors, CNS+RT patients had a trend toward lower relapse rates and reduced nonrelapse mortality. Conclusions: CNS+ AML is associated with a poor prognosis. The role of a cranial irradiation boost to intrathecal chemotherapy appears to mitigate the risk of CNS disease, and needs to be further investigated to define optimal treatment strategies.

  15. Asbestos-related pulmonary disease in boilermakers

    SciTech Connect (OSTI)

    Demers, R.Y.; Neale, A.V.; Robins, T.; Herman, S.C. )

    1990-01-01

    Boilermakers are skilled building tradesmen who construct, repair, and dismantle boilers. The present study reports on the evaluation of members of a Michigan boilermaker's union for the presence of signs and symptoms of chronic pulmonary disease. Study variables included standardized evaluations of chest x-ray findings, pulmonary function testing, physical examination, and respiratory symptoms. An overall participation rate of 69% was achieved. A non-participant survey identified no significant differences between participants and non-participants in dyspnea, cough, age, or smoking history. Among participants with greater than 20 years experience in the trade, the mean FVC was 91% of predicted; the FEV1 was 86% of predicted; 25% showed at least a 1/0 profusion of interstitial markings on chest x-ray; 30% had bilateral pleural abnormalities; and 52% had audible inspiratory rales on physical examination. Forced vital capacity and forced expiratory volume at one second both decreased with years in the trade. Chest x-ray findings of interstitial fibrosis and pleural plaques were related to ten or more years in the trade, as were respiratory symptoms of pulmonary rales, wheeze, and dyspnea.

  16. Polymorphic microsatellites and Wilson disease (WD)

    SciTech Connect (OSTI)

    Stewart, E.A.; Cavalli-Sforza, L.L. ); White, A.; Tomfohrde, J.; Osborne-Lawrence, S.; Prestidge, L.; Bowcock, A.M. ); Bonne-Tamir, B.; Scheinberg, I.H.; George-Hyslop, P.; Giagheddu, M.; Kim, J.W.; Seo, J.K.; Lo, W.H.Y.; Ivanova-Smolenskaya, I.A.; Limborska, S.A.; Farrer, L.A.

    1993-10-01

    Wilson disease (WD), an autosomal recessive disorder of copper metabolism, has been previously mapped to chromosome 13q. Highly informative PCR-based polymorphic microsatellites closely linked to the WD locus (WND) at 13q14.3, as well as sequence-tagged sites for closely linked loci, are described. Two polymorphic microsatellite markers at D13S118 and D13S119 lie within 3 cM of WND. Two others (D13S227 and D13S228) were derived from a yeast artificial chromosome containing D13S31. These were placed on a genetic linkage map of chromosome 13 and were typed in 74 multiplex WD families from a variety of geographic origins (166 affected members). Multipoint analysis provides very high odds that the location of WND is between D13S31/D13S227/D13S228 and D13S59. Previous odds with RFLP-based markers were only 7:1 more likely than any other location. Current odds are 5,000:1. Preclinical testing of three cases of WD by using the highly informative polymorphic microsatellite markers is described. The markers described ensure that 95% of predictive tests using DNA from both parents and from at least one affected sib will have an accuracy >99%. 29 refs., 2 figs., 3 tabs.

  17. OSTI, US Dept of Energy Office of Scientific and Technical Information |

    Office of Scientific and Technical Information (OSTI)

    Speeding access to science information from DOE and Beyond Sharing Data Leads to Progress by Nena Moss on Thu, August 19, 2010 My mother died in March 2010 after a 15-year battle with Alzheimer's, so I pay particular attention to news about this dreadful disease. A recent New York Times article caught my eye: "Sharing of Data Leads to Progress on Alzheimer's." How did sharing data lead to progress on Alzheimer's? A collaborative effort, the Alzheimer's Disease Neuroimaging

  18. Analyzing and modeling the kinetics of amyloid beta pores associated with Alzheimer’s disease pathology

    SciTech Connect (OSTI)

    Ullah, Ghanim; Demuro, Angelo; Parker, Ian; Pearson, John E.; Xu, Shang -Zhong

    2015-09-08

    Amyloid beta (Aβ) oligomers associated with Alzheimer’s disease (AD) form Ca2+-permeable plasma membrane pores, leading to a disruption of the otherwise well-controlled intracellular calcium (Ca2+) homeostasis. The resultant up-regulation of intracellular Ca2+ concentration has detrimental implications for memory formation and cell survival. The gating kinetics and Ca2+ permeability of Aβ pores are not well understood. We have used computational modeling in conjunction with the ability of optical patch-clamping for massively parallel imaging of Ca2+ flux through thousands of pores in the cell membrane of Xenopus oocytes to elucidate the kinetic properties of Aβ pores. The fluorescence time-series data from individual pores were idealized and used to develop data-driven Markov chain models for the kinetics of the Aβ pore at different stages of its evolution. Our study provides the first demonstration of developing Markov chain models for ion channel gating that are driven by optical-patch clamp data with the advantage of experiments being performed under close to physiological conditions. As a result, we demonstrate the up-regulation of gating of various Ca2+ release channels due to Aβ pores and show that the extent and spatial range of such up-regulation increases as Aβ pores with low open probability and Ca2+ permeability transition into those with high open probability and Ca2+ permeability.

  19. "Economic","per Employee","of Value Added","of Shipments" "Characteristic(a)","(million Btu)","(thousand Btu)","(thousand Btu)"

    U.S. Energy Information Administration (EIA) Indexed Site

    2 Relative Standard Errors for Table 6.2;" " Unit: Percents." ,,,"Consumption" " ",,"Consumption","per Dollar" " ","Consumption","per Dollar","of Value" "Economic","per Employee","of Value Added","of Shipments" "Characteristic(a)","(million Btu)","(thousand Btu)","(thousand Btu)" ,"Total United States" "Value

  20. "Economic","per Employee","of Value Added","of Shipments" "Characteristic(a)","(million Btu)","(thousand Btu)","(thousand Btu)"

    U.S. Energy Information Administration (EIA) Indexed Site

    2 Relative Standard Errors for Table 6.2;" " Unit: Percents." ,,,"Consumption" ,,"Consumption","per Dollar" ,"Consumption","per Dollar","of Value" "Economic","per Employee","of Value Added","of Shipments" "Characteristic(a)","(million Btu)","(thousand Btu)","(thousand Btu)" ,"Total United States" "Value of Shipments and

  1. Timing of testing and treatment for asymptomatic diseases

    SciTech Connect (OSTI)

    K?rk?zlar, Eser; Faissol, Daniel M.; Griffin, Paul M.; Swann, Julie L.

    2010-07-01

    Many papers in the medical literature analyze the cost-effectiveness of screening for diseases by comparing a limited number of a priori testing policies under estimated problem parameters. However, this may be insufficient to determine the best timing of the tests or incorporate changes over time. In this paper, we develop and solve a Markov Decision Process (MDP) model for a simple class of asymptomatic diseases in order to provide the building blocks for analysis of a more general class of diseases. We provide a computationally efficient method for determining a cost-effective dynamic intervention strategy that takes into account (i) the results of the previous test for each individual and (ii) the change in the individuals behavior based on awareness of the disease. We demonstrate the usefulness of the approach by applying the results to screening decisions for Hepatitis C (HCV) using medical data, and compare our findings to current HCV screening recommendations.

  2. Update on the Hanford Site Chronic Beryllium Disease Prevention...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by DOE- EM on March 13, 2013 2 Be CAP Status * Rev. 1 of the Hanford site wide Chronic Beryllium Disease Prevention Program (CBDPP) was approved by DOE March 7, 2012 * Rev....

  3. Array-based detection of genetic alterations associated with disease

    DOE Patents [OSTI]

    Pinkel, Daniel; Albertson, Donna G.; Gray, Joe W.

    2007-09-11

    The present invention relates to DNA sequences from regions of copy number change on chromosome 20. The sequences can be used in hybridization methods for the identification of chromosomal abnormalities associated with various diseases.

  4. Inexpensive handheld tests for diseases are in sight

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cai has a passion: to use her expertise in biochemistry and genetics to create a non-invasive, point-of-care diagnostic device for disease detection. In following her passion, she...

  5. Science On Tap - Understanding HIV and Infectious Diseases

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Science On Tap - Understanding HIV and Infectious Diseases Science On Tap - Understanding HIV and Infectious Diseases WHEN: Sep 17, 2015 5:30 PM - 7:00 PM WHERE: UnQuarked Wine Room 145 Central Park Square, Los Alamos, New Mexico, 87544 USA SPEAKER: Ruy Ribeiro, Los Alamos National Laboratory CONTACT: Jessica Privette 505 667-0375 CATEGORY: Bradbury INTERNAL: Calendar Login Science On Tap - Every Third Thursday Event Description Science On Tap happens every third Thursday of the month, featuring

  6. Engineering disease resistance with pectate lyase-like genes

    DOE Patents [OSTI]

    Vogel, John; Somerville, Shauna

    2005-03-08

    A mutant gene coding for pectate lyase and homologs thereof is provided, which when incorporated in transgenic plants effect an increased level disease resistance in such plants. Also is provided the polypeptide sequence for the pectate lyase of the present invention. Methods of obtaining the mutant gene, producing transgenic plants which include the nucleotide sequence for the mutant gene and producing improved disease resistance in a crop of such transgenic plants are also provided.

  7. LANL spinoff receives NIH grant for respiratory disease diagnostic device

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    LANL spinoff receives NIH grant LANL spinoff receives NIH grant for respiratory disease diagnostic device Mesa Tech has been awarded a grant to develop an inexpensive, instrument-free, nucleic-acid testing device to diagnose various respiratory diseases in record time. October 19, 2011 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable

  8. Software speeds detection of diseases and cancer-treatment targets

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Software speeds detection of diseases Software speeds detection of diseases and cancer-treatment targets The Lab has released an updated version of software that is now capable of identifying DNA from viruses and all parts of the Tree of Life. December 1, 2014 With Sequedex, a laptop computer can analyze DNA sequences faster than any current DNA sequencer can create them. With Sequedex, a laptop computer can analyze DNA sequences faster than any current DNA sequencer can create them. Contact

  9. Cerebrospinal Fluid Proteome of Patients with Acute Lyme Disease

    SciTech Connect (OSTI)

    Angel, Thomas E.; Jacobs, Jon M.; Smith, Robert P.; Pasternack, Mark S.; Elias, Susan; Gritsenko, Marina A.; Shukla, Anil K.; Gilmore, Edward C.; McCarthy, Carol; Camp, David G.; Smith, Richard D.

    2012-10-05

    Acute Lyme disease results from transmission of and infection by the bacterium Borrelia burgdorferi following a tick bite. During acute infection, bacteria can disseminate to the central nervous system (CNS) leading to the development of Lyme meningitis. Here we have analyzed pooled cerebrospinal fluid (CSF) allowing for a deep view into the proteome for a cohort of patients with early-disseminated Lyme disease and CSF inflammation leading to the identification of proteins that reflect host responses, which are distinct for subjects with acute Lyme disease. Additionally, we analyzed individual patient samples and quantified changes in protein abundance employing label-free quantitative mass spectrometry based methods. The measured changes in protein abundances reflect the impact of acute Lyme disease on the CNS as presented in CSF. We have identified 89 proteins that differ significantly in abundance in patients with acute Lyme disease. A number of the differentially abundant proteins have been found to be localized to brain synapse and thus constitute important leads for better understanding of the neurological consequence of disseminated Lyme disease.

  10. Airborne spread of foot-and-mouth disease - model intercomparison

    SciTech Connect (OSTI)

    Gloster, J; Jones, A; Redington, A; Burgin, L; Sorensen, J H; Turner, R; Dillon, M; Hullinger, P; Simpson, M; Astrup, P; Garner, G; Stewart, P; D'Amours, R; Sellers, R; Paton, D

    2008-09-04

    Foot-and-mouth disease is a highly infectious vesicular disease of cloven-hoofed animals caused by foot-and-mouth disease virus. It spreads by direct contact between animals, by animal products (milk, meat and semen), by mechanical transfer on people or fomites and by the airborne route - with the relative importance of each mechanism depending on the particular outbreak characteristics. Over the years a number of workers have developed or adapted atmospheric dispersion models to assess the risk of foot-and-mouth disease virus spread through the air. Six of these models were compared at a workshop hosted by the Institute for Animal Health/Met Office during 2008. A number of key issues emerged from the workshop and subsequent modelling work: (1) in general all of the models predicted similar directions for 'at risk' livestock with much of the remaining differences strongly related to differences in the meteorological data used; (2) determination of an accurate sequence of events is highly important, especially if the meteorological conditions vary substantially during the virus emission period; and (3) differences in assumptions made about virus release, environmental fate, and subsequent infection can substantially modify the size and location of the downwind risk area. Close relationships have now been established between participants, which in the event of an outbreak of disease could be readily activated to supply advice or modelling support.

  11. Remote sensing, global warming, and vector-borne disease

    SciTech Connect (OSTI)

    Wood, B.; Beck, L.; Dister, S.; Lobitz, B.

    1997-12-31

    The relationship between climate change and the pattern of vector-borne disease can be viewed at a variety of spatial and temporal scales. At one extreme are changes such as global warming, which are continental in scale and occur over periods of years, decades, or longer. At the opposite extreme are changes associated with severe weather events, which can occur at local and regional scales over periods of days, weeks, or months. Key ecological factors affecting the distribution of vector-borne diseases include temperature, precipitation, and habitat availability, and their impact on vectors, pathogens, reservoirs, and hosts. Global warming can potentially alter these factors, thereby affecting the spatial and temporal patterns of disease.

  12. Investigation of saliva of patients with periodontal disease using NAA

    SciTech Connect (OSTI)

    Zamboni, C. B.; Metairon, S.; Medeiros, I. M. M. A.

    2013-05-06

    In this study the non-stimulated whole saliva of 26 healthy subjects (mean age 33.9 {+-} 11.0 years, range: 26 to 49 years) and 11 patients with periodontal disease (mean age 41.7 {+-} 11.5 years; range 29 to 55 years) was investigated using Neutron Activation Analysis (NAA) technique. The samples were obtained from donors at Sao Paulo city (Brazil). The analyses were performed in the nuclear reactor IEA-R1 (3.5-4.5MW, pool type) at IPEN/CNEN-SP (Brazil). Considerable changes in Ca and S saliva's level were identified in patients with periodontal disease suggesting they can be used as monitors of periodontal diseases.

  13. Disease Diagnostics Research at Los Alamos National Laboratory

    SciTech Connect (OSTI)

    Mukundan, Harshini

    2015-04-10

    Human evolution and persistent diseases have existed side-by-side. A recent concern is the re-emergence of tuberculosis, one of the oldest and most challenging diseases known to man. Effective diagnosis can save lives and prevent its spread. This talk will cover how our immune system discriminates between itself and foreign entities and how a new laboratory and nature inspired strategy can detect tuberculosis equally well in human and animal populations. The approach is being extended to other applications such as the identification of strep throat and respiratory infections.

  14. Device, array, and methods for disease detection and analysis

    DOE Patents [OSTI]

    Rao, Rupa S.; Lane, Stephen M.; Matthews, Dennis L.; Coleman, Matthew A.

    2016-06-14

    A device and array coupled to capture molecules are provided. Specifically, the device and array can be used for detecting the presence and concentration of biomarkers in a sample from a subject. The device and array can also allow the use of a method for scoring a sample for, e.g., the purpose of diagnosing a disease. The method can also be advantageous to applications where there is a need to accurately determine the disease stage of a subject for the purpose of making therapeutic decisions.

  15. Compositions and Methods for the Treatment of Pierce's Disease

    DOE Patents [OSTI]

    Gupta, Goutam

    2008-10-07

    Chimeric anti-microbial proteins, compositions, and methods for the therapeutic and prophylactic treatment of plant diseases caused by the bacterial pathogen Xylella fastidiosa are provided. The anti-microbial proteins of the invention generally comprise a surface recognition domain polypeptide, capable of binding to a bacterial membrane component, fused to a bacterial lysis domain polypeptide, capable of affecting lysis or rupture of the bacterial membrane, typically via a fused polypeptide linker. In particular, methods and compositions for the treatment or prevention of Pierce's disease of grapevines are provided. Methods for the generation of transgenic Vitus vinefera plants expressing xylem-secreted anti-microbial chimeras are also provided.

  16. Frequency adding lasers and optical amplifiers

    SciTech Connect (OSTI)

    Goldstone, J.A.

    1989-02-21

    A method is described for creating population inversions for use in stimulated emission devices which beam combine, beam cleanup, or frequency up-convert electromagnetic radiation, comprising: (a) providing a multilevel quantum medium; (b) providing pump beams for near resonantly enhanced multiphoton pumping of the multilevel quantum medium, the multilevel quantum medium having atoms or molecules with three or more energy levels whose transition energies are near resonant with the pump beams, the near resonantly enhanced multiphoton pumping being defined such that each pump beam photon energy is close to but not equal to a transition energy between two energy levels of the multilevel quantum medium, thus producing small single photon detunings between the pump beams and the energy levels, these small single photon detunings thereby defining interacting energy levels of the multilevel quantum medium; and directing the pump beams into the multilevel quantum medium having decay rates into an intermediate energy level greater than decay rates out of the intermediate energy level, the intermediate energy level lying between the lowest and highest of the interacting energy levels.

  17. GS-AD-0010-001.PDF

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    10-001.doc Forklift Date: 2001/01/17 This directive concerns the use of the CAMD forklift by individuals who have not received training by CAMD (for example a vendor) but who need to operate the CAMD forklift to ensure completion their work (contract) at CAMD. This option is available only to outside contractors. Reference to this exception is detailed in GS-PO-0009- 001.doc under section 8, entitled Exemptions to the rule. All individuals described above, must sign a release waiver. A sample

  18. ARM - Value-Added Products (VAP)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    VAPs, which have been updated to the ARM quality control standards, produce both c1 and s1 output files (see ARM file naming conventions). The s1 files are summary files that...

  19. Adding International Rates | OpenEI Community

    Open Energy Info (EERE)

    is this possible ? Submitted by Simonberry on 17 March, 2014 - 06:24 1 answer Points: 0 Hello, I provide user support for SAM and was asked by the OpenEI team to post a response to...

  20. Ad Lucem Workshop: Modeling Breakout Session

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    "In the very general and abstract use of the term to date, there has been little necessity to understand what a 'market' might actually consist of or what a 'transformation' might look like.... When viewed through the lens of scientific theory and empirical research, we find that markets are complex human systems of exchange, deeply embedded in a variety of socio-political and cultural systems, all supported by long-lived technical infrastructure (which has been shaped historically by a

  1. Doppler Lidar Wind Value-Added Product

    SciTech Connect (OSTI)

    Newsom, R. K.; Sivaraman, C.; Shippert, T. R.; Riihimaki, L. D.

    2015-07-01

    Wind speed and direction, together with pressure, temperature, and relative humidity, are the most fundamental atmospheric state parameters. Accurate measurement of these parameters is crucial for numerical weather prediction. Vertically resolved wind measurements in the atmospheric boundary layer are particularly important for modeling pollutant and aerosol transport. Raw data from a scanning coherent Doppler lidar system can be processed to generate accurate height-resolved measurements of wind speed and direction in the atmospheric boundary layer.

  2. GS-AD-0009-001.PDF

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    09-001.doc Crane Date: 20010130 This directive concerns the use of the CAMD crane by individuals who have not received training by CAMD (for example a vendor) but who need to ...

  3. ARM - Value-Added Products (VAP)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ASI, AWR, ENA, FKB, GRW, HFE, MAO, NIM, NSA, PGH, PVC, PYE, SGP, SMT, TWP 2005.03.23 ... Properties AWR, FKB, GRW, HFE, MAO, NIM, NSA, PGH, PVC, PYE, SGP 1996.07.02 2016.09.30 ...

  4. ADDING REALISM TO NUCLEAR MATERIAL DISSOLVING ANALYSIS

    SciTech Connect (OSTI)

    Williamson, B.

    2011-08-15

    Two new criticality modeling approaches have greatly increased the efficiency of dissolver operations in H-Canyon. The first new approach takes credit for the linear, physical distribution of the mass throughout the entire length of the fuel assembly. This distribution of mass is referred to as the linear density. Crediting the linear density of the fuel bundles results in using lower fissile concentrations, which allows higher masses to be charged to the dissolver. Also, this approach takes credit for the fact that only part of the fissile mass is wetted at a time. There are multiple assemblies stacked on top of each other in a bundle. On average, only 50-75% of the mass (the bottom two or three assemblies) is wetted at a time. This means that only 50-75% (depending on operating level) of the mass is moderated and is contributing to the reactivity of the system. The second new approach takes credit for the progression of the dissolving process. Previously, dissolving analysis looked at a snapshot in time where the same fissile material existed both in the wells and in the bulk solution at the same time. The second new approach models multiple consecutive phases that simulate the fissile material moving from a high concentration in the wells to a low concentration in the bulk solution. This approach is more realistic and allows higher fissile masses to be charged to the dissolver.

  5. Microsoft Word - Rapp-AD.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hampton, Virginia P. Minnis, W. L. Smith, Jr., L. Nguyen, and M. P. Haeffelin NASA Langley ... This proceedings. Haeffelin, M., S. Kato, A. M. Smith, C. K. Rutledge, T. P. Charlock, and ...

  6. Value-Added Stock Loan Participation Program

    Broader source: Energy.gov [DOE]

    Like Minnesota's Agricultural Improvement Loan Program, this is a "participation loan" program, where loans are made by individual financial institutions working with the Rural Finance Authority ...

  7. Azasugar inhibitors as pharmacological chaperones for Krabbe disease

    SciTech Connect (OSTI)

    Hill, Chris H.; Viuff, Agnete H.; Spratley, Samantha J.; Salamone, Stéphane; Christensen, Stig H.; Read, Randy J.; Moriarty, Nigel W.; Jensen, Henrik H.; Deane, Janet E.

    2015-03-23

    Krabbe disease is a devastating neurodegenerative disorder characterized by rapid demyelination of nerve fibers. This disease is caused by defects in the lysosomal enzyme β-galactocerebrosidase (GALC), which hydrolyzes the terminal galactose from glycosphingolipids. These lipids are essential components of eukaryotic cell membranes: substrates of GALC include galactocerebroside, the primary lipid component of myelin, and psychosine, a cytotoxic metabolite. Mutations of GALC that cause misfolding of the protein may be responsive to pharmacological chaperone therapy (PCT), whereby small molecules are used to stabilize these mutant proteins, thus correcting trafficking defects and increasing residual catabolic activity in cells. Here we describe a new approach for the synthesis of galacto-configured azasugars and the characterization of their interaction with GALC using biophysical, biochemical and crystallographic methods. We identify that the global stabilization of GALC conferred by azasugar derivatives, measured by fluorescence-based thermal shift assays, is directly related to their binding affinity, measured by enzyme inhibition. X-ray crystal structures of these molecules bound in the GALC active site reveal which residues participate in stabilizing interactions, show how potency is achieved and illustrate the penalties of aza/iminosugar ring distortion. The structure–activity relationships described here identify the key physical properties required of pharmacological chaperones for Krabbe disease and highlight the potential of azasugars as stabilizing agents for future enzyme replacement therapies. This work lays the foundation for new drug-based treatments of Krabbe disease.

  8. Ventilation assessment of an infectious disease ward housing TB patients

    SciTech Connect (OSTI)

    Crandall, M.S.; Hughes, R.T.

    1996-05-01

    The National Institute for Occupational Safety and Health (NIOSH) assisted the National Center for Infectious Diseases and the National Center for Prevention Services, Centers for Disease Control (CDC), in their investigation of nosocomial transmission of tuberculosis (TB) at a Veterans Administration Medical Center. NIOSH was asked to determine whether ventilation requirements expected of TB patient isolation facilities were being met. In the Infectious Disease ward (513), 24 staff were given a tuberculin skin test (TST) in the summer of 1991. Eleven (46%) were positive then, and 13 were negative. Ten of the 13 testing negative in 1991 were retested within a year, and 5 (50%) converted to a positive TST. NIOSH investigators made ventilation measurements on Ward 5B, an infectious diseases ward housing patients with acquired immune deficiency syndrome (AIDS), two of them with infectious TB, to determine the status of the systems serving the area. Airflow measurements showed that in all the single-patient rooms, exhaust airflow was essentially zero. The average supply airflow varied above and below the designed value. These rooms were all positively pressurized, which would be recommended for the isolation of infectious patients. Based on the measurements made during this evaluation, it was recommended that a separate isolation facility be constructed in the hospital to house infectious patients. Interim corrective measures for the systems in place were also recommended.

  9. Azasugar inhibitors as pharmacological chaperones for Krabbe disease

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hill, Chris H.; Viuff, Agnete H.; Spratley, Samantha J.; Salamone, Stéphane; Christensen, Stig H.; Read, Randy J.; Moriarty, Nigel W.; Jensen, Henrik H.; Deane, Janet E.

    2015-03-23

    Krabbe disease is a devastating neurodegenerative disorder characterized by rapid demyelination of nerve fibers. This disease is caused by defects in the lysosomal enzyme β-galactocerebrosidase (GALC), which hydrolyzes the terminal galactose from glycosphingolipids. These lipids are essential components of eukaryotic cell membranes: substrates of GALC include galactocerebroside, the primary lipid component of myelin, and psychosine, a cytotoxic metabolite. Mutations of GALC that cause misfolding of the protein may be responsive to pharmacological chaperone therapy (PCT), whereby small molecules are used to stabilize these mutant proteins, thus correcting trafficking defects and increasing residual catabolic activity in cells. Here we describe amore » new approach for the synthesis of galacto-configured azasugars and the characterization of their interaction with GALC using biophysical, biochemical and crystallographic methods. We identify that the global stabilization of GALC conferred by azasugar derivatives, measured by fluorescence-based thermal shift assays, is directly related to their binding affinity, measured by enzyme inhibition. X-ray crystal structures of these molecules bound in the GALC active site reveal which residues participate in stabilizing interactions, show how potency is achieved and illustrate the penalties of aza/iminosugar ring distortion. The structure–activity relationships described here identify the key physical properties required of pharmacological chaperones for Krabbe disease and highlight the potential of azasugars as stabilizing agents for future enzyme replacement therapies. This work lays the foundation for new drug-based treatments of Krabbe disease.« less

  10. Small heat shock proteins protect against {alpha}-synuclein-induced toxicity and aggregation

    SciTech Connect (OSTI)

    Outeiro, Tiago Fleming [Alzheimer's Research Unit, MassGeneral Institute for Neurodegenerative Disease, MGH, Harvard Medical School, CNY 114, 16th Street, Charlestown, MA 02129 (United States); Klucken, Jochen [Alzheimer's Research Unit, MassGeneral Institute for Neurodegenerative Disease, MGH, Harvard Medical School, CNY 114, 16th Street, Charlestown, MA 02129 (United States); Strathearn, Katherine E. [Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907-2091 (United States); Liu Fang [Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907-2091 (United States); Nguyen, Paul [Alzheimer's Research Unit, MassGeneral Institute for Neurodegenerative Disease, MGH, Harvard Medical School, CNY 114, 16th Street, Charlestown, MA 02129 (United States); Rochet, Jean-Christophe [Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907-2091 (United States); Hyman, Bradley T. [Alzheimer's Research Unit, MassGeneral Institute for Neurodegenerative Disease, MGH, Harvard Medical School, CNY 114, 16th Street, Charlestown, MA 02129 (United States); McLean, Pamela J. [Alzheimer's Research Unit, MassGeneral Institute for Neurodegenerative Disease, MGH, Harvard Medical School, CNY 114, 16th Street, Charlestown, MA 02129 (United States)]. E-mail: touteiro@partners.org

    2006-12-22

    Protein misfolding and inclusion formation are common events in neurodegenerative diseases, such as Parkinson's disease (PD), Alzheimer's disease (AD) or Huntington's disease (HD). {alpha}-Synuclein (aSyn) is the main protein component of inclusions called Lewy bodies (LB) which are pathognomic of PD, Dementia with Lewy bodies (DLB), and other diseases collectively known as LB diseases. Heat shock proteins (HSPs) are one class of the cellular quality control system that mediate protein folding, remodeling, and even disaggregation. Here, we investigated the role of the small heat shock proteins Hsp27 and {alpha}B-crystallin, in LB diseases. We demonstrate, via quantitative PCR, that Hsp27 messenger RNA levels are {approx}2-3-fold higher in DLB cases compared to control. We also show a corresponding increase in Hsp27 protein levels. Furthermore, we found that Hsp27 reduces aSyn-induced toxicity by {approx}80% in a culture model while {alpha}B-crystallin reduces toxicity by {approx}20%. In addition, intracellular inclusions were immunopositive for endogenous Hsp27, and overexpression of this protein reduced aSyn aggregation in a cell culture model.

  11. The Human Phenotype Ontology: Semantic Unification of Common and Rare Disease

    SciTech Connect (OSTI)

    Groza, Tudor; Khler, Sebastian; Moldenhauer, Dawid; Vasilevsky, Nicole; Baynam, Gareth; Zemojtel, Tomasz; Schriml, LynnMarie; Kibbe, WarrenAlden; Schofield, PaulN.; Beck, Tim; Vasant, Drashtti; Brookes, AnthonyJ.; Zankl, Andreas; Washington, NicoleL.; Mungall, ChristopherJ.; Lewis, SuzannaE.; Haendel, Melissa A.; Parkinson, Helen; Robinson, PeterN.

    2015-06-25

    The Human Phenotype Ontology (HPO) is widely used in the rare disease community for differential diagnostics, phenotype-driven analysis of next-generation sequence-variation data, and translational research, but a comparable resource has not been available for common disease. Here, we have developed a concept-recognition procedure that analyzes the frequencies of HPO disease annotations as identified in over five million PubMed abstracts by employing an iterative procedure to optimize precision and recall of the identified terms. We derived disease models for 3,145 common human diseases comprising a total of 132,006 HPO annotations. The HPO now comprises over 250,000 phenotypic annotations for over 10,000 rare and common diseases and can be used for examining the phenotypic overlap among common diseases that share risk alleles, as well as between Mendelian diseases and common diseases linked by genomic location. The annotations, as well as the HPO itself, are freely available.

  12. Conversion efficiency improvement of inverted CH{sub 3}NH{sub 3}PbI{sub 3} perovskite solar cells with room temperature sputtered ZnO by adding the C{sub 60} interlayer

    SciTech Connect (OSTI)

    Lai, Wei-Chih Chen, Peter; Lin, Kun-Wei; Wang, Yuan-Ting; Guo, Tzung-Fang

    2015-12-21

    We have demonstrated the performance of inverted CH{sub 3}NH{sub 3}PbI{sub 3} perovskite-based solar cells (SCs) with a room temperature (RT) sputtered ZnO electron transport layer by adding fullerene (C{sub 60}) interlayer. ZnO exhibits a better matched conduction band level with perovskite and Al work function and around energy offset of 2.2 eV between highest occupied molecular orbital level of CH{sub 3}NH{sub 3}PbI{sub 3} perovskite and valance band level of ZnO. However, the CH{sub 3}NH{sub 3}PbI{sub 3} perovskite layer will be damaged during direct RT sputtering deposition of ZnO. Therefore, the C{sub 60} interlayer having matched conduction band level with ZnO and CH{sub 3}NH{sub 3}PbI{sub 3} perovskite added between the CH{sub 3}NH{sub 3}PbI{sub 3} perovskite and RT sputtered ZnO layers for protection prevents sputtering damages on the CH{sub 3}NH{sub 3}PbI{sub 3} perovskite layer. The short-circuit current density (J{sub SC}, 19.41 mA/cm{sup 2}) and open circuit voltage (V{sub OC}, 0.91 V) of the SCs with glass/ITO/poly(3,4-ethylenedioxythiophene):poly(styrene-sulfonate) (PEDOT:PSS)/perovskite/C{sub 60}/RT sputtered ZnO/Al structure is higher than the J{sub SC} (16.23 mA/cm{sup 2}) and V{sub OC} (0.90 V) of the reference SC with glass/ITO/PEDOT:PSS/perovskite/C{sub 60}/bathocuproine (BCP)/Al structure. Although the SCs with the former structure has a lower fill factor (FF%) than the SCs with the latter structure, its conversion efficiency η% (10.93%) is higher than that (10.6%) of the latter.

  13. Using Genomics to Study Human Biology and Disease

    SciTech Connect (OSTI)

    Myers, Ricard M.

    2005-04-06

    The Human Genome Project culminated in April 2003 with the finished DNA sequence of all of the human chromosomes. This book of information, particularly in conjunction with the genome sequences of many other organisms, has already begun to revolutionize the way that biomedical scientists study our species. The identification of essentially all of our genes has provided a template upon which researchers can discover basic processes that govern cells, organs, and the whole organism, and to understand the fundamental causes of the diseases that occur when something goes wrong with a gene or a set of genes. The Genome Project has already made it possible to identify the genes that are defective in more than 1,000 rare inherited diseases, and these discoveries have helped to understand the mechanisms of the more common forms of these disorders. This understanding of primary defects in diseases - which is translated as mutations in genes that encode proteins that serve specific functions - is transforming the way that biotechnology and pharmaceutical companies identify drug targets, and a few notable cases have already had a striking impact on specific diseases. In addition, it has become clear that the differential response to drugs in human populations is heavily influenced by genes, and a whole field called pharmacogenetics has begun to identify these genetic factors. Such knowledge will allow physicians to prescribe drugs targeted to each individual, with the potential to increase efficacy and decrease side-effects. Determining the DNA sequence of the human genome and identifying the genes has been an exciting endeavor, but we are only just beginning to understand the treasures present in all of our DNA. My presentation will briefly describe the road we took to get the sequence, as well as the tools that we are developing to unlock its secrets.

  14. Modeling the Molecular Basis of Parkinson's Disease | Argonne Leadership

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Computing Facility Alpha-synuclein pentamer constructed with 4ns molecular dynamics (MD) conformers after equilibration on the membrane with MD. Alpha-synuclein pentamer constructed with 4ns molecular dynamics (MD) conformers after equilibration on the membrane with MD. Modeling the Molecular Basis of Parkinson's Disease PI Name: Igor Tsigelny PI Email: itsigeln@ucsd.edu Institution: University of California-San Diego/SDSC Allocation Program: INCITE Allocation Hours at ALCF: 1.2 Million

  15. Radiological approaches in the evaluation of joint disease in children

    SciTech Connect (OSTI)

    Poznanski, A.K.; Conway, J.J.; Shkolnik, A.; Pachman, L.M.

    1987-04-01

    In summary, the newer technologies in radiology have allowed us to visualize more clearly the manifestation of joint disease in children. The presence of small erosions and cartilage damage can be seen much better with magnetic resonance imaging than with any other modality short of arthrography, a much more invasive examination. Joint effusion, although sometimes visualized with conventional radiography, is probably best recognized with ultrasound or magnetic resonance imaging, although it can be detected with computed tomography as well. For the evaluation of avascular necrosis that can be associated with steroid use in joint disease, bone scintigraphy is a simple, sensitive method. Magnetic resonance may be as or more sensitive and gives additional information as well. In the detection of change with time, conventional radiography probably will remain the standard as it is still the simplest, least expensive examination; however, it has many limitations in specific cases. Bone scintigraphy may be of value in selected cases. Although we have still not had enough experience with magnetic resonance imaging to use it as a way of evaluating progress of joint disease, it promises to be the most sensitive radiologic measure of evaluating progress as small anatomical changes can be detected within the cartilage, which cannot be done easily with other means. 27 references.

  16. Conductance control at the LaAlO{sub 3}/SrTiO{sub 3}-interface by a multiferroic BiFeO{sub 3} ad-layer

    SciTech Connect (OSTI)

    Mix, Christian; Finizio, Simone; Kläui, Mathias; Jakob, Gerhard

    2014-06-30

    Multilayered BiFeO{sub 3} (BFO)/LaAlO{sub 3} (LAO) thin film samples were fabricated on SrTiO{sub 3} (STO) substrates by pulsed laser deposition. In this work, the ferroelectric polarization of a multiferroic BFO ad-layer on top of the quasi-two-dimensional electron gas (2DEG) at the LAO/STO interface is used to manipulate the conductivity of the quasi-2DEG. By microstructuring the conductive area of the LAO/STO-interface, a four-point geometry for the measurement of the resistivity was achieved. Piezo force microscopy allows for imaging and poling the spontaneous ferroelectric polarization of the multiferroic layer. The resistance changes showed a linear dependence on the area scanned and a hysteretic behavior with respect to the voltages applied in the scanning process. This is evidence for the ferroelectric polarization of the multiferroic causing the resistance changes. Coupling the antiferromagnetic BFO layer to another ferromagnetic layer could enable a magnetic field control of the conductance of the quasi-2DEG at the LAO/STO interface.

  17. Vesicular Disease in 9-Week-Old Pigs Experimentally Infected with Senecavirus A

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Montiel, Nestor; Buckley, Alexandra; Guo, Baoqing; Kulshreshtha, Vikas; VanGeelen, Albert; Hoang, Hai; Rademacher, Christopher; Yoon, Kyoung-Jin; Lager, Kelly

    2016-07-01

    Senecavirus A has been infrequently associated with vesicular disease in swine since 1988. However, clinical disease has not been reproduced after experimental infection with this virus. Here we report vesicular disease in 9-week-old pigs after Sencavirus A infection by the intranasal route under experimental conditions.

  18. Evaluation of the fate and pathological response in the lung and pleura of brake dust alone and in combination with added chrysotile compared to crocidolite asbestos following short-term inhalation exposure

    SciTech Connect (OSTI)

    Bernstein, D.M.; Rogers, R.A.; Sepulveda, R.; Kunzendorf, P.; Bellmann, B.; Ernst, H.; Creutzenberg, O.; Phillips, J.I.

    2015-02-15

    This study was designed to provide an understanding of the biokinetics and potential toxicology in the lung and pleura following inhalation of brake dust following short term exposure in rats. The deposition, translocation and pathological response of brake-dust derived from brake pads manufactured with chrysotile were evaluated in comparison to the amphibole, crocidolite asbestos. Rats were exposed by inhalation 6 h/day for 5 days to either brake-dust obtained by sanding of brake-drums manufactured with chrysotile, a mixture of chrysotile and the brake-dust or crocidolite asbestos. The chrysotile fibers were relatively biosoluble whereas the crocidolite asbestos fibers persisted through the life-time of the animal. This was reflected in the lung and the pleura where no significant pathological response was observed at any time point in the brake dust or chrysotile/brake dust exposure groups through 365 days post exposure. In contrast, crocidolite asbestos produced a rapid inflammatory response in the lung parenchyma and the pleura, inducing a significant increase in fibrotic response in both of these compartments. Crocidolite fibers were observed embedded in the diaphragm with activated mesothelial cells immediately after cessation of exposure. While no chrysotile fibers were found in the mediastinal lymph nodes, crocidolite fibers of up to 35 μm were observed. These results provide support that brake-dust derived from chrysotile containing brake drums would not initiate a pathological response in the lung or the pleural cavity following short term inhalation. - Highlights: • Evaluated brake dust w/wo added chrysotile in comparison to crocidolite asbestos. • Persistence, translocation, pathological response in the lung and pleural cavity. • Chrysotile cleared rapidly from the lung while the crocidolite asbestos persisted. • No significant pathology in lung or pleural cavity observed at any time point in the brake-dust groups. • Crocidolite quickly

  19. Supratentorial Ependymoma: Disease Control, Complications, and Functional Outcomes After Irradiation

    SciTech Connect (OSTI)

    Landau, Efrat; Boop, Frederick A.; Conklin, Heather M.; Wu, Shengjie; Xiong, Xiaoping; Merchant, Thomas E.

    2013-03-15

    Purpose: Ependymoma is less commonly found in the supratentorial brain and has known clinical and molecular features that are unique. Our single-institution series provides valuable information about disease control for supratentorial ependymoma and the complications of supratentorial irradiation in children. Methods and Materials: A total of 50 children with newly diagnosed supratentorial ependymoma were treated with adjuvant radiation therapy (RT); conformal methods were used in 36 after 1996. The median age at RT was 6.5 years (range, 1-18.9 years). The entire group was characterized according to sex (girls 27), race (white 43), extent of resection (gross-total 46), and tumor grade (anaplastic 28). The conformal RT group was prospectively evaluated for neurologic, endocrine, and cognitive effects. Results: With a median follow-up time of 9.1 years from the start of RT for survivors (range, 0.2-23.2 years), the 10-year progression-free and overall survival were 73% + 7% and 76% + 6%, respectively. None of the evaluated factors was prognostic for disease control. Local and distant failures were evenly divided among the 16 patients who experienced progression. Eleven patients died of disease, and 1 of central nervous system necrosis. Seizure disorders were present in 17 patients, and 4 were considered to be clinically disabled. Clinically significant cognitive effects were limited to children with difficult-to-control seizures. The average values for intelligence quotient and academic achievement (reading, spelling, and math) were within the range of normal through 10 years of follow-up. Central hypothyroidism was the most commonly treated endocrinopathy. Conclusion: RT may be administered with acceptable risks for complications in children with supratentorial ependymoma. These results suggest that outcomes for these children are improving and that complications may be limited by use of focal irradiation methods.

  20. Development of Biomarkers for Chronic Beryllium Disease in Mice

    SciTech Connect (OSTI)

    Gordon, Terry

    2013-01-25

    Beryllium is a strategic metal, indispensable for national defense programs in aerospace, telecommunications, electronics, and weaponry. Exposure to beryllium is an extensively documented occupational hazard that causes irreversible, debilitating granulomatous lung disease in as much as 3 - 5% of exposed workers. Mechanistic research on beryllium exposure-disease relationships has been severely limited by a general lack of a sufficient CBD animal model. We have now developed and tested an animal model which can be used for dissecting dose-response relationships and pathogenic mechanisms and for testing new diagnostic and treatment paradigms. We have created 3 strains of transgenic mice in which the human antigen-presenting moiety, HLA-DP, was inserted into the mouse genome. Each mouse strain contains HLA-DPB1 alleles that confer different magnitude of risk for chronic beryllium disease (CBD): HLA-DPB1*0401 (odds ratio = 0.2), HLA-DPB1*0201 (odds ratio = 15), HLA-DPB1*1701 (odds ratio = 240). Our preliminary work has demonstrated that the *1701 allele, as predicted by human studies, results in the greatest degree of sensitization in a mouse ear swelling test. We have also completed dose-response experiments examining beryllium-induced lung granulomas and identified susceptible and resistant inbred strains of mice (without the human transgenes) as well as quantitative trait loci that may contain gene(s) that modify the immune response to beryllium. In this grant application, we propose to use the transgenic and normal inbred strains of mice to identify biomarkers for the progression of beryllium sensitization and CBD. To achieve this goal, we propose to compare the sensitivity and accuracy of the lymphocyte proliferation test (blood and bronchoalveolar lavage fluid) with the ELISPOT test in the three HLA-DP transgenic mice strains throughout a 6 month treatment with beryllium particles. Because of the availability of high-throughput proteomics, we will also identify

  1. Hanford Site Chronic Beryllium Disease Prevention Program (CBDPP) Contact List

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Chronic Beryllium Disease Prevention Program (CBDPP) Contact List Page 1 of 2 Updated 5/31/16 Primary and Alternate Membership Organization Primary Office Number Cell Number Alternate Office Number Cell Number BAG Fisher, Mark N/A 539-5588 Artzer, Josh 376-2463 430-5062 CHPRC/HAMTC Sherman, Larry 376-6436 438-2171 Spier, Tracy 373-1993 440-9118 CHPRC Bean, Tonya 376-6503 (303) 709-3047 Robinson, Roby Seydel, Scott (509) 699-9307 373-4860 (509) 699-9307 430-0184 CWB&CTC Park, Rick 372-9941

  2. Full-spectrum disease response : beyond just the flu.

    SciTech Connect (OSTI)

    Knazovich, Michael Ward; Cox, Warren B.; Henderson, Samuel Arthur

    2010-04-01

    Why plan beyond the flu: (1) the installation may be the target of bioterrorism - National Laboratory, military base collocated in large population center; and (2) International Airport - transport of infectious agents to the area - Sandia is a global enterprise and staff visit many foreign countries. In addition to the Pandemic Plan, Sandia has developed a separate Disease Response Plan (DRP). The DRP addresses Category A, B pathogens and Severe Acute Respiratory Syndrome (SARS). The DRP contains the Cities Readiness Initiative sub-plan for disbursement of Strategic National Stockpile assets.

  3. AdS Black Disk Model for Small-x Deep Inelastic Scattering

    SciTech Connect (OSTI)

    Cornalba, Lorenzo; Costa, Miguel S.; Penedones, Joao

    2010-08-13

    Using the approximate conformal invariance of QCD at high energies we consider a simple anti-de Sitter black disk model to describe saturation in deep inelastic scattering. Deep inside saturation the structure functions have the same power law scaling, F{sub T}{approx}F{sub L}{approx}x{sup -{omega}}, where {omega} is related to the expansion rate of the black disk with energy. Furthermore, the ratio F{sub L}/F{sub T} is given by the universal value (1+{omega}/3+{omega}), independently of the target. For {gamma}*-{gamma}* scattering at high energies we obtain explicit expressions and ratios for the total cross sections of transverse and longitudinal photons in terms of the single parameter {omega}.

  4. Osteogenic Sarcoma of the Maxilla: Neutron Therapy for Unresectable Disease

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Smoron, Geoffrey L.; Lennox, Arlene J.; Mcgee, James L.

    1999-01-01

    Purpose. To present a case study involving the use of fast neutron therapy to treat an extensive unresectable osteogenic sarcoma arising from the left maxilla. Patient. A 14-year-old male presented with a massive tumor producing severe distortion of his facial structures. He had already received six courses of chemotherapy, which had reduced his pain, but had not measurably reduced the tumor. Methods. The patient was treated with 66 MeV fast neutrons to a dose of 20.4 Gy in 13 fractions over 35 days. Results. CT assessments indicate gradually increasing calcification and noticeable reduction of soft-tissue disease in the frontalmore » sinus, orbit and maxillary antrum.There has been some recontouring of the facial structures.The boy conducts an active life, has no pain, and feels well. He was 17 years old at the last follow-up. Discussion. Fast neutrons have a greater biological effectiveness than conventional photon beams. Their use has been associated with improved chance for local control of unresectable disease.This case illustrates their effectiveness in controlling an unusual and aggressive osteogenic sarcoma of the facial bone and sinuses.« less

  5. Model Catalysis of Ammonia Synthesis ad Iron-Water Interfaces - ASum Frequency Generation Vibrational Spectroscopic Study of Solid-GasInterfaces and Anion Photoelectron Spectroscopic Study of Selected Anionclusters

    SciTech Connect (OSTI)

    Ferguson, Michael James

    2005-12-15

    The ammonia synthesis reaction has been studied using single crystal model catalysis combined with sum frequency generation (SFG) vibrational spectroscopy. The adsorption of gases N{sub 2}, H{sub 2}, O{sub 2} and NH{sub 3} that play a role in ammonia synthesis have been studied on the Fe(111) crystal surface by sum frequency generation vibrational spectroscopy using an integrated Ultra-High Vacuum (UHV)/high-pressure system. SFG spectra are presented for the dissociation intermediates, NH{sub 2} ({approx}3325 cm{sup -1}) and NH ({approx}3235 cm{sup -1}) under high pressure of ammonia or equilibrium concentrations of reactants and products on Fe(111) surfaces. Special attention was paid to understand how potassium promotion of the iron catalyst affects the intermediates of ammonia synthesis. An Fe(111) surface promoted with 0.2 monolayers of potassium red shifts the vibrational frequencies of the reactive surface intermediates, NH and NH{sub 2}, providing evidence for weakened the nitrogen-hydrogen bonds relative to clean Fe(111). Spectral features of these surface intermediates persisted to higher temperatures for promoted iron surfaces than for clean Fe(111) surfaces implying that nitrogen-iron bonds are stronger for the promoted surface. The ratio of the NH to NH{sub 2} signal changed for promoted surfaces in the presence of equilibrium concentrations of reactants and products. The order of adding oxygen and potassium to promoted surfaces does not alter the spectra indicating that ammonia induces surface reconstruction of the catalyst to produce the same surface morphology. When oxygen is co-adsorbed with nitrogen, hydrogen, ammonia or potassium on Fe(111), a relative phase shift of the spectra occurs as compared to the presence of adsorbates on clean iron surfaces. Water adsorption on iron was also probed using SFG vibrational spectroscopy. For both H{sub 2}O and D{sub 2}O, the only spectral feature was in the range of the free OH or free OD. From the absence of

  6. Bead-based microfluidic immunoassay for diagnosis of Johne's disease

    SciTech Connect (OSTI)

    Wadhwa, Ashutosh [University of Tennessee, Center for Wildlife Health, Department of Forestry; Foote, Robert [ORNL; Shaw, Robert W [ORNL; Eda, Shigetoshi [ORNL

    2012-01-01

    Microfluidics technology offers a platform for development of point-of-care diagnostic devices for various infectious diseases. In this study, we examined whether serodiagnosis of Johne s disease (JD) can be conducted in a bead-based microfluidic assay system. Magnetic micro-beads were coated with antigens of the causative agent of JD, Mycobacterium avium subsp. paratuberculosis. The antigen-coated beads were incubated with serum samples of JD-positive or negative serum samples and then with a fluorescently-labeled secondary antibody (SAB). To confirm binding of serum antibodies to the antigen, the beads were subjected to flow cytometric analysis. Different conditions (dilutions of serum and SAB, types of SAB, and types of magnetic beads) were optimized for a great degree of differentiation between the JD-negative and JD-positive samples. Using the optimized conditions, we tested a well-classified set of 155 serum samples from JD negative and JD-positive cattle by using the bead-based flow cytometric assay. Of 105 JD-positive samples, 63 samples (60%) showed higher antibody binding levels than a cut-off value determined by using antibody binding levels of JD-negative samples. In contrast, only 43-49 JD-positive samples showed higher antibody binding levels than the cut-off value when the samples were tested by commercially-available immunoassays. Microfluidic assays were performed by magnetically immobilizing a number of beads within a microchannel of a glass microchip and detecting antibody on the collected beads by laser-induced fluorescence. Antigen-coated magnetic beads treated with bovine serum sample and fluorescently-labeled SAB were loaded into a microchannel to measure the fluorescence (reflecting level of antibody binding) on the beads in the microfluidic system. When the results of five bovine serum samples obtained with the system were compared to those obtained with the flow cytometer, a high level of correlation (linear regression, r2 = 0.994) was

  7. Ulcerative colitis and steroid-responsive, diffuse interstitial lung disease

    SciTech Connect (OSTI)

    Balestra, D.J.; Balestra, S.T.; Wasson, J.H.

    1988-07-01

    The authors describe a patient with ulcerative colitis and extracolonic manifestations in whom diffuse interstitial pulmonary disease developed that was responsive to glucocorticoid therapy one year after total proctocolectomy. The patient presented in December 1983 with a subacute course marked by cough and progressive exertional dyspnea, abnormal chest examination results, and a chest roentgenogram that revealed diffuse interstitital and alveolar infiltrates. A transbronchial biopsy specimen revealed a polymorphic interstitial infiltrate, mild interstitial fibrosis without apparent intraluminal fibrosis, and no vasculitis, granulomas, or significant eosinophilic infiltration. Within one week of the initiation of daily high-dose steroid therapy, the patient's symptoms dramatically improved; chest roentgenogram and forced vital capacity (60%) improved at a slower rate. All three measures deteriorated when alternate-day prednisone therapy was started but once again improved until the patient was totally asymptomatic, chest roentgenograms were normal, and forced vital capacity was 80% of the predicted value 2 1/2 years later.

  8. Resource Requirements Planning for Hospitals Treating Serious Infectious Disease Cases.

    SciTech Connect (OSTI)

    Vugrin, Eric D.; Verzi, Stephen Joseph; Finley, Patrick D.; Turnquist, Mark A.; Wyte-Lake, Tamar; Griffin, Ann R.; Ricci, Karen J.; Plotinsky, Rachel

    2015-02-01

    This report presents a mathematical model of the way in which a hospital uses a variety of resources, utilities and consumables to provide care to a set of in-patients, and how that hospital might adapt to provide treatment to a few patients with a serious infectious disease, like the Ebola virus. The intended purpose of the model is to support requirements planning studies, so that hospitals may be better prepared for situations that are likely to strain their available resources. The current model is a prototype designed to present the basic structural elements of a requirements planning analysis. Some simple illustrati ve experiments establish the mo del's general capabilities. With additional inve stment in model enhancement a nd calibration, this prototype could be developed into a useful planning tool for ho spital administrators and health care policy makers.

  9. Amyloid-β-Anti-Amyloid-β Complex Structure Reveals an Extended Conformation in the Immunodominant B-Cell Epitope

    SciTech Connect (OSTI)

    Miles, Luke A; Wun, Kwok S; Crespi, Gabriela A.N.; Fodero-Tavoletti, Michelle T; Galatis, Denise; Bagley, Christopher J; Beyreuther, Konrad; Masters, Colin L; Cappai, Roberto; McKinstry, William J; Barnham, Kevin J; Parker, Michael W

    2012-04-17

    Alzheimer's disease (AD) is the most common form of dementia. Amyloid-β (Aβ) peptide, generated by proteolytic cleavage of the amyloid precursor protein, is central to AD pathogenesis. Most pharmaceutical activity in AD research has focused on Aβ, its generation and clearance from the brain. In particular, there is much interest in immunotherapy approaches with a number of anti-Aβ antibodies in clinical trials. We have developed a monoclonal antibody, called WO2, which recognises the Aβ peptide. To this end, we have determined the three-dimensional structure, to near atomic resolution, of both the antibody and the complex with its antigen, the Aβ peptide. The structures reveal the molecular basis for WO2 recognition and binding of Aβ. The Aβ peptide adopts an extended, coil-like conformation across its major immunodominant B-cell epitope between residues 2 and 8. We have also studied the antibody-bound Aβ peptide in the presence of metals known to affect its aggregation state and show that WO2 inhibits these interactions. Thus, antibodies that target the N-terminal region of Aβ, such as WO2, hold promise for therapeutic development.

  10. Edge effects, not connectivity, determine the incidence and development of a foliar fungal plant disease.

    SciTech Connect (OSTI)

    Johnson, Brenda, L.; Haddad, Nick, M.

    2011-08-01

    Using a model plant-pathogen system in a large-scale habitat corridor experiment, we found that corridors do not facilitate the movement of wind-dispersed plant pathogens, that connectivity of patches does not enhance levels of foliar fungal plant disease, and that edge effects are the key drivers of plant disease dynamics. Increased spread of infectious disease is often cited as a potential negative effect of habitat corridors used in conservation, but the impacts of corridors on pathogen movement have never been tested empirically. Using sweet corn (Zea mays) and southern corn leaf blight (Cochliobolus heterostrophus) as a model plant-pathogen system, we tested the impacts of connectivity and habitat fragmentation on pathogen movement and disease development at the Savannah River Site, South Carolina, USA. Over time, less edgy patches had higher proportions of diseased plants, and distance of host plants to habitat edges was the greatest determinant of disease development. Variation in average daytime temperatures provided a possible mechanism for these disease patterns. Our results show that worries over the potentially harmful effects of conservation corridors on disease dynamics are misplaced, and that, in a conservation context, many diseases can be better managed by mitigating edge effects.

  11. The Human Phenotype Ontology: Semantic Unification of Common and Rare Disease

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Groza, Tudor; Köhler, Sebastian; Moldenhauer, Dawid; Vasilevsky, Nicole; Baynam, Gareth; Zemojtel, Tomasz; Schriml, Lynn Marie; Kibbe, Warren Alden; Schofield, Paul N.; Beck, Tim; et al

    2015-06-25

    The Human Phenotype Ontology (HPO) is widely used in the rare disease community for differential diagnostics, phenotype-driven analysis of next-generation sequence-variation data, and translational research, but a comparable resource has not been available for common disease. Here, we have developed a concept-recognition procedure that analyzes the frequencies of HPO disease annotations as identified in over five million PubMed abstracts by employing an iterative procedure to optimize precision and recall of the identified terms. We derived disease models for 3,145 common human diseases comprising a total of 132,006 HPO annotations. The HPO now comprises over 250,000 phenotypic annotations for over 10,000more »rare and common diseases and can be used for examining the phenotypic overlap among common diseases that share risk alleles, as well as between Mendelian diseases and common diseases linked by genomic location. The annotations, as well as the HPO itself, are freely available.« less

  12. Peroxisome proliferator-activated receptor {gamma} is expressed in hippocampal neurons and its activation prevents {beta}-amyloid neurodegeneration: role of Wnt signaling

    SciTech Connect (OSTI)

    Inestrosa, Nibaldo C. . E-mail: ninestr@genes.bio.puc.cl; Godoy, Juan A.; Quintanilla, Rodrigo A.; Koenig, Cecilia S.; Bronfman, Miguel

    2005-03-10

    The molecular pathogenesis of Alzheimer's disease (AD) involves the participation of the amyloid-{beta}-peptide (A{beta}), which plays a critical role in the neurodegeneration that triggers the disease. Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors, which are members of the nuclear receptor family. We report here that (1) PPAR{gamma} is present in rat hippocampal neurons in culture. (2) Activation of PPAR{gamma} by troglitazone and rosiglitazone protects rat hippocampal neurons against A{beta}-induced neurodegeneration, as shown by the 3-[4,5 -2yl]-2,5-diphenyltetrazolium bromide (MTT) reduction assay, immunofluorescence using an anti-heavy neurofilament antibody, and quantitative electron microscopy. (3) Hippocampal neurons treated with several PPAR{gamma} agonists, including troglitazone, rosiglitazone, and ciglitazone, prevent the excitotoxic A{beta}-induced rise in bulk-free Ca{sup 2+}. (4) PPAR{gamma} activation results in the modulation of Wnt signaling components, including the inhibition of glycogen synthase kinase-3{beta} (GSK-3{beta}) and an increase of the cytoplasmic and nuclear {beta}-catenin levels. We conclude that the activation of PPAR{gamma} prevents A{beta}-induced neurodegeneration by a mechanism that may involve a cross talk between neuronal PPAR{gamma} and the Wnt signaling pathway. More important, the fact that the activation of PPAR{gamma} attenuated A{beta}-dependent neurodegeneration opens the possibility to fight AD from a new therapeutic perspective.

  13. Toxicological and pharmacological concerns on oxidative stress and related diseases

    SciTech Connect (OSTI)

    Saeidnia, Soodabeh; Abdollahi, Mohammad

    2013-12-15

    Although reactive oxygen species (ROS) such as superoxide, hydrogen peroxide and hydroxyl radical are generated as the natural byproduct of normal oxygen metabolism, they can create oxidative damage via interaction with bio-molecules. The role of oxidative stress as a remarkable upstream part is frequently reported in the signaling cascade of inflammation as well as chemo attractant production. Even though hydrogen peroxide can control cell signaling and stimulate cell proliferation at low levels, in higher concentrations it can initiate apoptosis and in very high levels may create necrosis. So far, the role of ROS in cellular damage and death is well documented with implicating in a broad range of degenerative alterations e.g. carcinogenesis, aging and other oxidative stress related diseases (OSRDs). Reversely, it is cleared that antioxidants are potentially able to suppress (at least in part) the immune system and to enhance the normal cellular protective responses to tissue damage. In this review, we aimed to provide insights on diverse OSRDs, which are correlated with the concept of oxidative stress as well as its cellular effects that can be inhibited by antioxidants. Resveratrol, angiotensin converting enzyme inhibitors, angiotensin receptor blockers, statins, nebivolol and carvedilol, pentaerythritol tetranitrate, mitochondria-targeted antioxidants, and plant-derived drugs (alone or combined) are the potential medicines that can be used to control OSRD.

  14. The temporal patterns of disease severity and prevalence in schistosomiasis

    SciTech Connect (OSTI)

    Ciddio, Manuela; Gatto, Marino Casagrandi, Renato

    2015-03-15

    Schistosomiasis is one of the most widespread public health problems in the world. In this work, we introduce an eco-epidemiological model for its transmission and dynamics with the purpose of explaining both intra- and inter-annual fluctuations of disease severity and prevalence. The model takes the form of a system of nonlinear differential equations that incorporate biological complexity associated with schistosome's life cycle, including a prepatent period in snails (i.e., the time between initial infection and onset of infectiousness). Nonlinear analysis is used to explore the parametric conditions that produce different temporal patterns (stationary, endemic, periodic, and chaotic). For the time-invariant model, we identify a transcritical and a Hopf bifurcation in the space of the human and snail infection parameters. The first corresponds to the occurrence of an endemic equilibrium, while the latter marks the transition to interannual periodic oscillations. We then investigate a more realistic time-varying model in which fertility of the intermediate host population is assumed to seasonally vary. We show that seasonality can give rise to a cascade of period-doubling bifurcations leading to chaos for larger, though realistic, values of the amplitude of the seasonal variation of fertility.

  15. Insight into Alzheimer's, cancer, anemia gleaned from ribosome...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and (B) computer-generated atomic-resolution model ... Color bar indicates the amount of motion during rolling. ... for modeling the structure and dynamic aspects of ...

  16. Insight into Alzheimer's, cancer, anemia gleaned from ribosome research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Inside the Crystal Ball: New Approaches to Predicting the Gasoline Price at the Pump Christiane Baumeister, Bank of Canada Lutz Kilian, University of Michigan Thomas K. Lee, U.S. Energy Information Administration April 2015 Independent Statistics & Analysis www.eia.gov U.S. Energy Information Administration Washington, DC 20585 This paper is released to encourage discussion and critical comment. The analysis and conclusions expressed here are those of the authors and not necessarily those of

  17. Multi-analyte analysis of saliva biomarkers as predictors of periodontal and pre-implant disease

    DOE Patents [OSTI]

    Braun, Thomas; Giannobile, William V; Herr, Amy E; Singh, Anup K; Shelburne, Charlie

    2015-04-07

    The present invention relates to methods of measuring biomarkers to determine the probability of a periodontal and/or peri-implant disease. More specifically, the invention provides a panel of biomarkers that, when used in combination, can allow determination of the probability of a periodontal and/or peri-implant disease state with extremely high accuracy.

  18. Milk production and distribution in low-dose counties for the Hanford Thyroid Disease Study

    SciTech Connect (OSTI)

    Schimmel, J.G. . Social and Economic Sciences Research Center); Beck, D.M. )

    1992-06-01

    This report identifies sources of milk consumed by residents of Ferry, Okanogan, and Stevens Counties. This information will be used by the Hanford thyroid Disease Study to determine whether thyroid disease has been increased among people exposed to past iodine--131 emissions from Hanford Site Facilities.

  19. Proceedings of the 2nd workshop on lyme disease in the Southeast

    SciTech Connect (OSTI)

    Apperson, C.S.; Levine, J.F.; Snoddy, E.L.

    1993-12-31

    This volume provides author prepared abstracts of oral presentation at the Second Workshop on Lyme Disease in the Southeast head in Raleigh, North Carolina September 7-9, 1993. The 33 presentations covered various aspects of the epidemic including geographical distribution of various species of ticks, transmission risks, Lyme Disease epidemiology, and taxonomic aspects.

  20. Delays in Reducing Waterborne and Water-related Infectious Diseases in China under Climate Change

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hodges, Maggie; Belle, Jessica; Carlton, Elizabeth; Liang, Song; Li, Huazhong; Luo, Wei; Freeman, Matthew C.; Liu, Yang; Gao, Yang; Hess, Jeremy; et al

    2014-11-02

    Despite China’s rapid progress improving water, sanitation and hygiene (WSH) infrastructure and access, in 2011, 471 million people lacked access to improved sanitation, and 401 million people lacked access to household piped water. Infectious diseases are sensitive to changes in climate, particularly temperature, and WSH conditions. To explore possible impacts of climate change on these diseases in China in 2020 and 2030, we coupled estimates of the temperature sensitivity of diarrheal disease and three vector-borne diseases, temperature projections from global climate models using four emissions pathways, WSH-infrastructure development scenarios and projected demographic changes. By 2030, the projected impacts would delaymore » China’s historically rapid progress toward reducing the burden of WSH-attributable infectious disease by 8-85 months. This developmental delay provides a key summary measure of the impact of climate change in China, and in other societies undergoing rapid social, economic, and environmental change.« less

  1. Delays in Reducing Waterborne and Water-related Infectious Diseases in China under Climate Change

    SciTech Connect (OSTI)

    Hodges, Maggie; Belle, Jessica; Carlton, Elizabeth; Liang, Song; Li, Huazhong; Luo, Wei; Freeman, Matthew C.; Liu, Yang; Gao, Yang; Hess, Jeremy; Remais, Justin V.

    2014-11-02

    Despite China’s rapid progress improving water, sanitation and hygiene (WSH) infrastructure and access, in 2011, 471 million people lacked access to improved sanitation, and 401 million people lacked access to household piped water. Infectious diseases are sensitive to changes in climate, particularly temperature, and WSH conditions. To explore possible impacts of climate change on these diseases in China in 2020 and 2030, we coupled estimates of the temperature sensitivity of diarrheal disease and three vector-borne diseases, temperature projections from global climate models using four emissions pathways, WSH-infrastructure development scenarios and projected demographic changes. By 2030, the projected impacts would delay China’s historically rapid progress toward reducing the burden of WSH-attributable infectious disease by 8-85 months. This developmental delay provides a key summary measure of the impact of climate change in China, and in other societies undergoing rapid social, economic, and environmental change.

  2. Delays in Reducing Waterborne and Water-related Infectious Diseases in China under Climate Change

    SciTech Connect (OSTI)

    Hodges, Maggie; Belle, Jessica; Carlton, Elizabeth; Liang, Song; Li, Huazhong; Luo, Wei; Freeman, Matthew C.; Liu, Yang; Gao, Yang; Hess, Jeremy; Remais, Justin V.

    2014-12-01

    Despite China’s rapid progress improving water, sanitation and hygiene (WSH) infrastructure and access, in 2011, 471 million people lacked access to improved sanitation, and 401 million people lacked access to household piped water. Infectious diseases are sensitive to changes in climate, particularly temperature, and WSH conditions. To explore possible impacts of climate change on these diseases in China in 2020 and 2030, we coupled estimates of the temperature sensitivity of diarrheal disease and three vector-borne diseases, temperature projections from global climate models using four emissions pathways, WSH-infrastructure development scenarios and projected demographic changes. By 2030, the projected impacts would delay China’s historically rapid progress toward reducing the burden of WSH-attributable infectious disease by 8-85 months. This developmental delay provides a key summary measure of the impact of climate change in China, and in other societies undergoing rapid social, economic, and environmental change.

  3. Health Detectives: Uncovering the Mysteries of Disease (LBNL Science at the Theater)

    ScienceCinema (OSTI)

    Bissell, Mina; Canaria, Christie; Celnicker, Susan; Karpen, Gary

    2014-05-06

    In this April 23, 2012 Science at the Theater event, Berkeley Lab scientists discuss how they uncover the mysteries of disease in unlikely places. Speakers and topics include: World-renowned cancer researcher Mina Bissell's pioneering research on the role of the cellular microenvironment in breast cancer has changed the conversation about the disease. How does DNA instability cause disease? To find out, Christie Canaria images neural networks to study disorders such as Huntington's disease. Fruit flies can tell us a lot about ourselves. Susan Celniker explores the fruit fly genome to learn how our genome works. DNA is not destiny. Gary Karpen explores how environmental factors shape genome function and disease through epigenetics.

  4. Mineral density volume gradients in normal and diseased human tissues

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Djomehri, Sabra I.; Candell, Susan; Case, Thomas; Browning, Alyssa; Marshall, Grayson W.; Yun, Wenbing; Lau, S. H.; Webb, Samuel; Ho, Sunita P.; Aikawa, Elena

    2015-04-09

    Clinical computed tomography provides a single mineral density (MD) value for heterogeneous calcified tissues containing early and late stage pathologic formations. The novel aspect of this study is that, it extends current quantitative methods of mapping mineral density gradients to three dimensions, discretizes early and late mineralized stages, identifies elemental distribution in discretized volumes, and correlates measured MD with respective calcium (Ca) to phosphorus (P) and Ca to zinc (Zn) elemental ratios. To accomplish this, MD variations identified using polychromatic radiation from a high resolution micro-computed tomography (micro-CT) benchtop unit were correlated with elemental mapping obtained from a microprobe X-raymore » fluorescence (XRF) using synchrotron monochromatic radiation. Digital segmentation of tomograms from normal and diseased tissues (N=5 per group; 40-60 year old males) contained significant mineral density variations (enamel: 2820-3095mg/cc, bone: 570-1415mg/cc, cementum: 1240-1340mg/cc, dentin: 1480-1590mg/cc, cementum affected by periodontitis: 1100-1220mg/cc, hypomineralized carious dentin: 345-1450mg/cc, hypermineralized carious dentin: 1815-2740mg/cc, and dental calculus: 1290-1770mg/cc). A plausible linear correlation between segmented MD volumes and elemental ratios within these volumes was established, and Ca/P ratios for dentin (1.49), hypomineralized dentin (0.32-0.46), cementum (1.51), and bone (1.68) were observed. Furthermore, varying Ca/Zn ratios were distinguished in adapted compared to normal tissues, such as in bone (855-2765) and in cementum (595-990), highlighting Zn as an influential element in prompting observed adaptive properties. Hence, results provide insights on mineral density gradients with elemental concentrations and elemental footprints that in turn could aid in elucidating mechanistic processes for pathologic formations.« less

  5. Mineral density volume gradients in normal and diseased human tissues

    SciTech Connect (OSTI)

    Djomehri, Sabra I.; Candell, Susan; Case, Thomas; Browning, Alyssa; Marshall, Grayson W.; Yun, Wenbing; Lau, S. H.; Webb, Samuel; Ho, Sunita P.; Aikawa, Elena

    2015-04-09

    Clinical computed tomography provides a single mineral density (MD) value for heterogeneous calcified tissues containing early and late stage pathologic formations. The novel aspect of this study is that, it extends current quantitative methods of mapping mineral density gradients to three dimensions, discretizes early and late mineralized stages, identifies elemental distribution in discretized volumes, and correlates measured MD with respective calcium (Ca) to phosphorus (P) and Ca to zinc (Zn) elemental ratios. To accomplish this, MD variations identified using polychromatic radiation from a high resolution micro-computed tomography (micro-CT) benchtop unit were correlated with elemental mapping obtained from a microprobe X-ray fluorescence (XRF) using synchrotron monochromatic radiation. Digital segmentation of tomograms from normal and diseased tissues (N=5 per group; 40-60 year old males) contained significant mineral density variations (enamel: 2820-3095mg/cc, bone: 570-1415mg/cc, cementum: 1240-1340mg/cc, dentin: 1480-1590mg/cc, cementum affected by periodontitis: 1100-1220mg/cc, hypomineralized carious dentin: 345-1450mg/cc, hypermineralized carious dentin: 1815-2740mg/cc, and dental calculus: 1290-1770mg/cc). A plausible linear correlation between segmented MD volumes and elemental ratios within these volumes was established, and Ca/P ratios for dentin (1.49), hypomineralized dentin (0.32-0.46), cementum (1.51), and bone (1.68) were observed. Furthermore, varying Ca/Zn ratios were distinguished in adapted compared to normal tissues, such as in bone (855-2765) and in cementum (595-990), highlighting Zn as an influential element in prompting observed adaptive properties. Hence, results provide insights on mineral density gradients with elemental concentrations and elemental footprints that in turn could aid in elucidating mechanistic processes for pathologic formations.

  6. Disease mapping based on stochastic SIR-SI model for Dengue and Chikungunya in Malaysia

    SciTech Connect (OSTI)

    Samat, N. A.; Ma'arof, S. H. Mohd Imam

    2014-12-04

    This paper describes and demonstrates a method for relative risk estimation which is based on the stochastic SIR-SI vector-borne infectious disease transmission model specifically for Dengue and Chikungunya diseases in Malaysia. Firstly, the common compartmental model for vector-borne infectious disease transmission called the SIR-SI model (susceptible-infective-recovered for human populations; susceptible-infective for vector populations) is presented. This is followed by the explanations on the stochastic SIR-SI model which involve the Bayesian description. This stochastic model then is used in the relative risk formulation in order to obtain the posterior relative risk estimation. Then, this relative estimation model is demonstrated using Dengue and Chikungunya data of Malaysia. The viruses of these diseases are transmitted by the same type of female vector mosquito named Aedes Aegypti and Aedes Albopictus. Finally, the findings of the analysis of relative risk estimation for both Dengue and Chikungunya diseases are presented, compared and displayed in graphs and maps. The distribution from risk maps show the high and low risk area of Dengue and Chikungunya diseases occurrence. This map can be used as a tool for the prevention and control strategies for both diseases.

  7. Using social media for actionable disease surveillance and outbreak management. A systematic literature review

    SciTech Connect (OSTI)

    Charles-Smith, Lauren E.; Reynolds, Tera L.; Cameron, Mark A.; Conway, Mike; Lau, Eric H. Y.; Olsen, Jennifer M.; Pavlin, Julie A.; Shigematsu, Mika; Streichert, Laura C.; Suda, Katie J.; Corley, Courtney D.; Braunstein, Lidia Adriana

    2015-10-05

    Here, research studies show that social media may be valuable tools in the disease surveillance toolkit used for improving public health professionals’ ability to detect disease outbreaks faster than traditional methods and to enhance outbreak response. A social media work group, consisting of surveillance practitioners, academic researchers, and other subject matter experts convened by the International Society for Disease Surveillance, conducted a systematic primary literature review using the PRISMA framework to identify research, published through February 2013, answering either of the following questions: 1) Can social media be integrated into disease surveillance practice and outbreak management to support and improve public health? 2) Can social media be used to effectively target populations, specifically vulnerable populations, to test an intervention and interact with a community to improve health outcomes? Examples of social media included are Facebook, MySpace, microblogs (e.g., Twitter), blogs, and discussion forums. For Question 1, 33 manuscripts were identified, starting in 2009 with topics on Influenza-like Illnesses (n=15), Infectious Diseases (n = 6), Non-infectious Diseases (n=4), Medication and Vaccines (n=3), and Other (n=5). For Question 2, 32 manuscripts were identified, the first in 2000 with topics on Health Risk Behaviors (n=10), Infectious Diseases (n = 3), Non-infectious Diseases (n=9), and Other (n=10). The literature on the use of social media to support public health practice has identified many gaps and biases in current knowledge. Despite the potential for success identified in exploratory studies, there are limited studies on interventions and little use of social media in practice. However, information gleaned from the articles demonstrates the effectiveness of social media in supporting and improving public health and in identifying target populations for intervention. A primary recommendation resulting from the review is to

  8. Using social media for actionable disease surveillance and outbreak management. A systematic literature review

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Charles-Smith, Lauren E.; Reynolds, Tera L.; Cameron, Mark A.; Conway, Mike; Lau, Eric H. Y.; Olsen, Jennifer M.; Pavlin, Julie A.; Shigematsu, Mika; Streichert, Laura C.; Suda, Katie J.; et al

    2015-10-05

    Here, research studies show that social media may be valuable tools in the disease surveillance toolkit used for improving public health professionals’ ability to detect disease outbreaks faster than traditional methods and to enhance outbreak response. A social media work group, consisting of surveillance practitioners, academic researchers, and other subject matter experts convened by the International Society for Disease Surveillance, conducted a systematic primary literature review using the PRISMA framework to identify research, published through February 2013, answering either of the following questions: 1) Can social media be integrated into disease surveillance practice and outbreak management to support and improvemore » public health? 2) Can social media be used to effectively target populations, specifically vulnerable populations, to test an intervention and interact with a community to improve health outcomes? Examples of social media included are Facebook, MySpace, microblogs (e.g., Twitter), blogs, and discussion forums. For Question 1, 33 manuscripts were identified, starting in 2009 with topics on Influenza-like Illnesses (n=15), Infectious Diseases (n = 6), Non-infectious Diseases (n=4), Medication and Vaccines (n=3), and Other (n=5). For Question 2, 32 manuscripts were identified, the first in 2000 with topics on Health Risk Behaviors (n=10), Infectious Diseases (n = 3), Non-infectious Diseases (n=9), and Other (n=10). The literature on the use of social media to support public health practice has identified many gaps and biases in current knowledge. Despite the potential for success identified in exploratory studies, there are limited studies on interventions and little use of social media in practice. However, information gleaned from the articles demonstrates the effectiveness of social media in supporting and improving public health and in identifying target populations for intervention. A primary recommendation resulting from the review

  9. Using Social Media for Actionable Disease Surveillance and Outbreak Management: A Systematic Literature Review

    SciTech Connect (OSTI)

    Charles-Smith, Lauren E.; Reynolds, Tera; Cameron, Mark; Conway, Mike; Lau, Eric H.; Olsen, Jennifer; Pavlin, Julie A.; Shigematsu, Mika; Streichert, Laura; Suda, Katie; Corley, Courtney D.

    2015-10-05

    Research studies show that social media may be valuable tools in the disease surveillance toolkit used for improving public health professionals ability to detect disease outbreaks faster than traditional methods and to enhance outbreak response. A social media work group, consisting of surveillance practitioners, academic researchers, and other subject matter experts convened by the International Society for Disease Surveillance, conducted a systematic primary literature review using the PRISMA framework to identify research, published through February 2013, answering either of the following questions: 1) Can social media be integrated into disease surveillance practice and outbreak management to support and improve public health? 2) Can social media be used to effectively target populations, specifically vulnerable populations, to test an intervention and interact with a community to improve health outcomes? Examples of social media included are Facebook, MySpace, microblogs (e.g., Twitter), blogs, and discussion forums. For Question 1, 33 manuscripts were identified, starting in 2009 with topics on Influenza-like Illnesses (n=15), Infectious Diseases (n = 6), Non-infectious Diseases (n=4), Medication and Vaccines (n=3), and Other (n=5). For Question 2, 32 manuscripts were identified, the first in 2000 with topics on Health Risk Behaviors (n=10), Infectious Diseases (n = 3), Non-infectious Diseases (n=9), and Other (n=10). The literature on the use of social media to support public health practice has identified many gaps and biases in current knowledge. Despite the potential for success identified in exploratory studies, there are limited studies on interventions and little use of social media in practice. However, information gleaned from the articles demonstrates the effectiveness of social media in supporting and improving public health and in identifying target populations for intervention. A primary recommendation resulting from the review is to identify

  10. Whole-Genome Sequences of Two Borrelia afzelii and Two Borrelia garinii Lyme Disease Agent Isolates

    SciTech Connect (OSTI)

    Casjens, S.R.; Dunn, J.; Mongodin, E. F.; Qiu, W.-G.; Luft, B. J.; Fraser-Liggett, C. M.; Schutzer, S. E.

    2011-12-01

    Human Lyme disease is commonly caused by several species of spirochetes in the Borrelia genus. In Eurasia these species are largely Borrelia afzelii, B. garinii, B. burgdorferi, and B. bavariensis sp. nov. Whole-genome sequencing is an excellent tool for investigating and understanding the influence of bacterial diversity on the pathogenesis and etiology of Lyme disease. We report here the whole-genome sequences of four isolates from two of the Borrelia species that cause human Lyme disease, B. afzelii isolates ACA-1 and PKo and B. garinii isolates PBr and Far04.

  11. Integrating Heterogeneous Healthcare Datasets and Visual Analytics for Disease Bio-surveillance and Dynamics

    SciTech Connect (OSTI)

    Ramanathan, Arvind; Pullum, Laura L; Steed, Chad A; Quinn, Shannon; Chennubhotla, Chakra; Parker, Tara L

    2013-01-01

    n this paper, we present an overview of the big data chal- lenges in disease bio-surveillance and then discuss the use of visual analytics for integrating data and turning it into knowl- edge. We will explore two integration scenarios: (1) combining text and multimedia sources to improve situational awareness and (2) enhancing disease spread model data with real-time bio-surveillance data. Together, the proposed integration methodologies can improve awareness about when, where and how emerging diseases can affect wide geographic regions.

  12. Speaker for Nov. 1 Lecture to Discuss Deadliest Viral Diseases | Jefferson

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Lab Speaker for Nov. 1 Lecture to Discuss Deadliest Viral Diseases Speaker for Nov. 1 Lecture to Discuss Deadliest Viral Diseases NEWPORT NEWS, Va., Oct. 27, 2011 - A noted scientist will discuss some of the world's deadliest viruses as guest speaker at Jefferson Lab's Fall Science Series lecture to be held at the lab on Tuesday, Nov. 1. Susan Fisher-Hoch, a virologist and epidemiologist from the University of Texas School of Public Health will present: Studying Deadly Diseases. She will

  13. Global warming and the potential spread of vector-borne diseases

    SciTech Connect (OSTI)

    Patz, J.

    1996-12-31

    Climatic factors influence many vector-borne infectious diseases, in addition to demographic, biological, and ecological determinants. The United Nation`s Intergovernmental Panel on Climate Change (IPCC) estimates an unprecedented global rise of 2.0 C by the year 2100. Of major concern is that these changes can affect the spread of many serious infectious diseases, including malaria and dengue fever. Global warming would directly affect disease transmission by shifting the mosquito`s geographic range, increasing reproductive and biting rates, and shortening pathogen incubation period. Human migration and damage to health infrastructures from the projected increase in climate variability and sea level rise could indirectly contribute to disease transmission. A review of this literature, as well as preliminary data from ongoing studies will be presented.

  14. "NAICS",,"per Employee","of Value Added","of Shipments" "Code(a)","Economic Characteristic(b)","(million Btu)","(thousand Btu)","(thousand Btu)"

    U.S. Energy Information Administration (EIA) Indexed Site

    3 Relative Standard Errors for Table 6.3;" " Unit: Percents." " "," ",,,"Consumption" " "," ",,"Consumption","per Dollar" " "," ","Consumption","per Dollar","of Value" "NAICS",,"per Employee","of Value Added","of Shipments" "Code(a)","Economic Characteristic(b)","(million Btu)","(thousand

  15. "NAICS",,"per Employee","of Value Added","of Shipments" "Code(a)","Economic Characteristic(b)","(million Btu)","(thousand Btu)","(thousand Btu)"

    U.S. Energy Information Administration (EIA) Indexed Site

    4 Relative Standard Errors for Table 6.4;" " Unit: Percents." " "," ",,,"Consumption" " "," ",,"Consumption","per Dollar" " "," ","Consumption","per Dollar","of Value" "NAICS",,"per Employee","of Value Added","of Shipments" "Code(a)","Economic Characteristic(b)","(million Btu)","(thousand

  16. "NAICS",,"per Employee","of Value Added","of Shipments" "Code(a)","Economic Characteristic(b)","(million Btu)","(thousand Btu)","(thousand Btu)"

    U.S. Energy Information Administration (EIA) Indexed Site

    3 Relative Standard Errors for Table 6.3;" " Unit: Percents." ,,,,"Consumption" ,,,"Consumption","per Dollar" ,,"Consumption","per Dollar","of Value" "NAICS",,"per Employee","of Value Added","of Shipments" "Code(a)","Economic Characteristic(b)","(million Btu)","(thousand Btu)","(thousand Btu)" ,,"Total United States" "

  17. "NAICS",,"per Employee","of Value Added","of Shipments" "Code(a)","Economic Characteristic(b)","(million Btu)","(thousand Btu)","(thousand Btu)"

    U.S. Energy Information Administration (EIA) Indexed Site

    4 Relative Standard Errors for Table 6.4;" " Unit: Percents." ,,,,"Consumption" ,,,"Consumption","per Dollar" ,,"Consumption","per Dollar","of Value" "NAICS",,"per Employee","of Value Added","of Shipments" "Code(a)","Economic Characteristic(b)","(million Btu)","(thousand Btu)","(thousand Btu)" ,,"Total United States" "

  18. In utero and early life arsenic exposure in relation to long-term health and disease

    SciTech Connect (OSTI)

    Farzan, Shohreh F.; Karagas, Margaret R.; Chen, Yu

    2013-10-15

    Background: There is a growing body of evidence that prenatal and early childhood exposure to arsenic from drinking water can have serious long-term health implications. Objectives: Our goal was to understand the potential long-term health and disease risks associated with in utero and early life exposure to arsenic, as well as to examine parallels between findings from epidemiological studies with those from experimental animal models. Methods: We examined the current literature and identified relevant studies through PubMed by using combinations of the search terms “arsenic”, “in utero”, “transplacental”, “prenatal” and “fetal”. Discussion: Ecological studies have indicated associations between in utero and/or early life exposure to arsenic at high levels and increases in mortality from cancer, cardiovascular disease and respiratory disease. Additional data from epidemiologic studies suggest intermediate effects in early life that are related to risk of these and other outcomes in adulthood. Experimental animal studies largely support studies in humans, with strong evidence of transplacental carcinogenesis, atherosclerosis and respiratory disease, as well as insight into potential underlying mechanisms of arsenic's health effects. Conclusions: As millions worldwide are exposed to arsenic and evidence continues to support a role for in utero arsenic exposure in the development of a range of later life diseases, there is a need for more prospective studies examining arsenic's relation to early indicators of disease and at lower exposure levels. - Highlights: • We review in utero and early-life As exposure impacts on lifelong disease risks. • Evidence indicates that early-life As increases risks of lung disease, cancer and CVD. • Animal work largely parallels human studies and may lead to new research directions. • Prospective studies and individual exposure assessments with biomarkers are needed. • Assessing intermediary endpoints may

  19. Independent Oversight Inspection of the Hanford Site Chronic Beryllium Disease Prevention Program

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Independent Oversight Inspection of the Hanford Site Chronic Beryllium Disease Prevention Program June 2010 Office of Independent Oversight Office of Health, Safety and Security Office of Health, Safety and Security HSS x Hanford Site Chronic Beryllium Disease Prevention Program | i Abbreviations iii Executive Summary v 1 Introduction 1 2 Management and Oversight 3 3 Findings Requiring Corrective Action 14 4 Conclusions and Cross-Cutting Opportunities for Improvement 17 Appendix A - Supplemental

  20. Periostin differentially induces proliferation, contraction and apoptosis of primary Dupuytren's disease and adjacent palmar fascia cells

    SciTech Connect (OSTI)

    Vi, Linda; Feng, Lucy; Zhu, Rebecca D.; Wu, Yan; Lawson Health Research Institute, London, Ontario ; Satish, Latha; Gan, Bing Siang; Lawson Health Research Institute, London, Ontario; Department of Surgery, University of Western Ontario, London, Ontario; Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario; Department of Medical Biophysics, University of Western Ontario, London, Ontario ; O'Gorman, David B.

    2009-12-10

    Dupuytren's disease, (DD), is a fibroproliferative condition of the palmar fascia in the hand, typically resulting in permanent contracture of one or more fingers. This fibromatosis is similar to scarring and other fibroses in displaying excess collagen secretion and contractile myofibroblast differentiation. In this report we expand on previous data demonstrating that POSTN mRNA, which encodes the extra-cellular matrix protein periostin, is up-regulated in Dupuytren's disease cord tissue relative to phenotypically normal palmar fascia. We demonstrate that the protein product of POSTN, periostin, is abundant in Dupuytren's disease cord tissue while little or no periostin immunoreactivity is evident in patient-matched control tissues. The relevance of periostin up-regulation in DD was assessed in primary cultures of cells derived from diseased and phenotypically unaffected palmar fascia from the same patients. These cells were grown in type-1 collagen-enriched culture conditions with or without periostin addition to more closely replicate the in vivo environment. Periostin was found to differentially regulate the apoptosis, proliferation, {alpha} smooth muscle actin expression and stressed Fibroblast Populated Collagen Lattice contraction of these cell types. We hypothesize that periostin, secreted by disease cord myofibroblasts into the extra-cellular matrix, promotes the transition of resident fibroblasts in the palmar fascia toward a myofibroblast phenotype, thereby promoting disease progression.

  1. Percutaneous Cholangioscopy in the Management of Biliary Disease: Experience in 25 Patients

    SciTech Connect (OSTI)

    Hatzidakis, Adam A.; Alexandrakis, George; Kouroumalis, Helias; Gourtsoyiannis, Nicholas C.

    2000-11-15

    Purpose: To present our experience performing percutaneous cholangioscopy in the management of 25 patients with biliary disease.Methods: During the last 3 years, 26 percutaneous cholangioscopies were performed in 25 patients with common bile duct disease (n = 16), intrahepatic ducts disease (n = 6), and gallbladder disease (n = 4). Our patient population group included seven with common bile duct stones, three with intrahepatic lithiasis, and eight with benign strictures (six iatrogenic and two postinflammatory). In four patients malignancy was to be excluded, in two the tumor extent was to be evaluated, whereas in one case the correct placement of a metallic stent needed to be controlled. A 9.9 Fr flexible endoscope URF-P (Olympus, 1.2 mm working channel, 70-cm length) was used.Results: In total, percutaneous cholangioscopy answered 30 diagnostic questions, was technically helpful in 19 cases (performing lithotripsy or biopsy or guiding a wire), and of therapeutic help in 12 (performing stone retrieval). In 24 of 26 cases the therapeutic decision and the patient management changed because of the findings or because of the help of the method. In two cases biliary intervention failed to treat the cause of the disease. No major complication due to the use of the endoscopy was noted.Conclusions: Percutaneous cholangioscopy is a very useful tool in the management of patients with biliary disease. The method can help in diagnosis, in performing complex interventional procedures, and in making or changing therapeutic decisions.

  2. Solid-state NMR analysis of the {beta}-strand orientation of the protofibrils of amyloid {beta}-protein

    SciTech Connect (OSTI)

    Doi, Takashi; Masuda, Yuichi; Irie, Kazuhiro; Akagi, Ken-ichi; Monobe, Youko; Imazawa, Takayoshi; Takegoshi, K.

    2012-11-30

    Highlights: Black-Right-Pointing-Pointer The supramolecular structure of A{beta}42 protofibrils was analyzed by solid-state NMR. Black-Right-Pointing-Pointer The Ala-21 residue in the A{beta}42 protofibrils is included in a slightly disordered {beta}-strand. Black-Right-Pointing-Pointer The A{beta}42 protofibrils do not form intermolecular in-register parallel {beta}-sheets. -- Abstract: Alzheimer's disease (AD) is caused by abnormal deposition (fibrillation) of a 42-residue amyloid {beta}-protein (A{beta}42) in the brain. During the process of fibrillation, the A{beta}42 takes the form of protofibrils with strong neurotoxicity, and is thus believed to play a crucial role in the pathogenesis of AD. To elucidate the supramolecular structure of the A{beta}42 protofibrils, the intermolecular proximity of the Ala-21 residues in the A{beta}42 protofibrils was analyzed by {sup 13}C-{sup 13}C rotational resonance experiments in the solid state. Unlike the A{beta}42 fibrils, an intermolecular {sup 13}C-{sup 13}C correlation was not found in the A{beta}42 protofibrils. This result suggests that the {beta}-strands of the A{beta}42 protofibrils are not in an in-register parallel orientation. A{beta}42 monomers would assemble to form protofibrils with the {beta}-strand conformation, then transform into fibrils by forming intermolecular parallel {beta}-sheets.

  3. 17β-trenbolone, an anabolic–androgenic steroid as well as an environmental hormone, contributes to neurodegeneration

    SciTech Connect (OSTI)

    Ma, Fucui; Liu, Daicheng

    2015-01-01

    Both genetic and environmental factors contribute to neurodegenerative disorders. In a large number of neurodegenerative diseases (for example, Alzheimer's disease (AD)), patients do not carry the mutant genes. Other risk factors, for example the environmental factors, should be evaluated. 17β-trenbolone is a kind of environmental hormone as well as an anabolic–androgenic steroid. 17β-trenbolone is used as a growth promoter for livestock in the USA. Also, a large portion of recreational exercisers inject 17β-trenbolone in large doses and for very long time to increase muscle and strength. 17β-trenbolone is stable in the environment after being excreted. In the present study, 17β-trenbolone was administered to adult and pregnant rats and the primary hippocampal neurons. 17β-trenbolone's distribution and its effects on serum hormone levels and Aβ42 accumulation in vivo and its effects on AD related parameters in vitro were assessed. 17β-trenbolone accumulated in adult rat brain, especially in the hippocampus, and in the fetus brain. It altered Aβ42 accumulation. 17β-trenbolone induced apoptosis of primary hippocampal neurons in vitro and resisted neuroprotective function of testosterone. Presenilin-1 protein expression was down-regulated while β-amyloid peptide 42 (Aβ42) production and caspase-3 activities were increased. Both androgen and estrogen receptors mediated the processes. 17β-trenbolone played critical roles in neurodegeneration. Exercisers who inject large doses of trenbolone and common people who are exposed to 17β-trenbolone by various ways are all influenced chronically and continually. Identification of such environmental risk factors will help us take early prevention measure to slow down the onset of neurodegenerative disorders. - Highlights: • The widely used anabolic–androgenic steroid 17β-trenbolone has neurotoxicity. • 17β-trenbolone crosses the blood brain barrier and placental barrier. • Rat has high level of 17

  4. HepatoProteomics: Applying Proteomic Technologies to the Study of Liver Function and Disease

    SciTech Connect (OSTI)

    Diamond, Deborah L.; Proll, Sean; Jacobs, Jon M.; Chan, Eric Y.; Camp, David G.; Smith, Richard D.; Katze, Michael G.

    2006-08-01

    The wealth of human genome sequence information now available, coupled with technological advances in robotics, nanotechnology, mass spectrometry, and information systems, has given rise to a method of scientific inquiry known as functional genomics. By using these technologies to survey gene expression and protein production on a near global scale, the goal of functional genomics is to assign biological function to genes with currently unknown roles in physiology. This approach carries particular appeal in disease research, where it can uncover the function of previously unknown genes and molecular pathways that are directly involved in disease progression. With this knowledge may come improved diagnostic techniques, prognostic capabilities, and novel therapeutic approaches. In this regard, the continuing evolution of proteomic technologies has resulted in an increasingly greater impact of proteome studies in many areas of research and hepatology is no exception. Our laboratory has been extremely active in this area, applying both genomic and proteomic technologies to the analysis of virus-host interactions in several systems, including the study of hepatitis C virus (HCV) infection and HCV-associated liver disease. Since proteomic technologies are foreign to many hepatologists (and to almost everyone else), this article will provide an overview of proteomic methods and technologies and describe how they're being used to study liver function and disease. We use our studies of HCV infection and HCV-associated liver disease to present an operational framework for performing high throughput proteome analysis and extracting biologically meaningful information.

  5. The Biosurveillance Analytics Resource Directory (BARD): Facilitating the use of epidemiological models for infectious disease surveillance

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Margevicius, Kristen J.; Generous, Nicholas; Abeyta, Esteban; Althouse, Ben; Burkom, Howard; Castro, Lauren; Daughton, Ashlynn; Del Valle, Sara Y.; Fairchild, Geoffrey; Hyman, James M.; et al

    2016-01-28

    Epidemiological modeling for infectious disease is important for disease management and its routine implementation needs to be facilitated through better description of models in an operational context. A standardized model characterization process that allows selection or making manual comparisons of available models and their results is currently lacking. A key need is a universal framework to facilitate model description and understanding of its features. Los Alamos National Laboratory (LANL) has developed a comprehensive framework that can be used to characterize an infectious disease model in an operational context. The framework was developed through a consensus among a panel of subjectmore » matter experts. In this paper, we describe the framework, its application to model characterization, and the development of the Biosurveillance Analytics Resource Directory (BARD; http://brd.bsvgateway.org/brd/), to facilitate the rapid selection of operational models for specific infectious/communicable diseases. We offer this framework and associated database to stakeholders of the infectious disease modeling field as a tool for standardizing model description and facilitating the use of epidemiological models.« less

  6. Multiplexed Molecular Assays for Rapid Rule-Out of Foot-and-Mouth Disease

    SciTech Connect (OSTI)

    Lenhoff, R; Naraghi-Arani, P; Thissen, J; Olivas, J; Carillo, C; Chinn, C; Rasmussen, M; Messenger, S; Suer, L; Smith, S M; Tammero, L; Vitalis, E; Slezak, T R; Hullinger, P J; Hindson, B J; Hietala, S; Crossley, B; Mcbride, M

    2007-06-26

    A nucleic acid-based multiplexed assay was developed that combines detection of foot-and-mouth disease virus (FMDV) with rule-out assays for two other foreign animal diseases and four domestic animal diseases that cause vesicular or ulcerative lesions indistinguishable from FMDV infection in cattle, sheep and swine. The FMDV 'look-alike' diagnostic assay panel contains five PCR and twelve reverse transcriptase PCR (RT-PCR) signatures for a total of seventeen simultaneous PCR amplifications for seven diseases plus incorporating four internal assay controls. It was developed and optimized to amplify both DNA and RNA viruses simultaneously in a single tube and employs Luminex{trademark} liquid array technology. Assay development including selection of appropriate controls, a comparison of signature performance in single and multiplex testing against target nucleic acids, as well of limits of detection for each of the individual signatures is presented. While this assay is a prototype and by no means a comprehensive test for FMDV 'look-alike' viruses, an assay of this type is envisioned to have benefit to a laboratory network in routine surveillance and possibly for post-outbreak proof of freedom from foot-and-mouth disease.

  7. Respiratory diseases research at NIOSH: reviews of research programs of the National Institute for Occupational Safety and Health

    SciTech Connect (OSTI)

    2008-07-01

    Respiratory diseases caused by exposures to dangerous materials in the workplace have tremendous implications for worker health and, by extension, the national economy. The National Institute for Occupational Safety and Health (NIOSH) estimates that deaths from work-related respiratory diseases and cancers account for about 70% of all occupational disease deaths. NIOSH conducts research in order to detect and reduce work-related hazardous exposures, injuries, and diseases; its Respiratory Disease Research Program (RDRP) focuses on respiratory diseases. This National Research Council book reviews the RDRP to evaluate the 1) relevance of its work to improvements in occupational safety and health and 2) the impact of research in reducing workplace respiratory illnesses. The assessment reveals that the program has made essential contributions to preventing occupational respiratory disease. The National Research Council has rated the Program a 5 out of 5 for relevance, and a 4 out of 5 for impact. To further increase its effectiveness, the Respiratory Disease Research Program should continue and expand its current efforts, provide resources for occupational disease surveillance, and include exposure assessment scientists in its activities. There are numerous references to respiratory systems diseases caused by coal mining. 4 apps.

  8. Introduction to Focus Issue: Rhythms and Dynamic Transitions in Neurological Disease: Modeling, Computation, and Experiment

    SciTech Connect (OSTI)

    Kaper, Tasso J. Kramer, Mark A.; Rotstein, Horacio G.

    2013-12-15

    Rhythmic neuronal oscillations across a broad range of frequencies, as well as spatiotemporal phenomena, such as waves and bumps, have been observed in various areas of the brain and proposed as critical to brain function. While there is a long and distinguished history of studying rhythms in nerve cells and neuronal networks in healthy organisms, the association and analysis of rhythms to diseases are more recent developments. Indeed, it is now thought that certain aspects of diseases of the nervous system, such as epilepsy, schizophrenia, Parkinson's, and sleep disorders, are associated with transitions or disruptions of neurological rhythms. This focus issue brings together articles presenting modeling, computational, analytical, and experimental perspectives about rhythms and dynamic transitions between them that are associated to various diseases.

  9. A Systems Biology Approach to Infectious Disease Research: Innovating the Pathogen-Host Research Paradigm

    SciTech Connect (OSTI)

    Aderem, Alan; Adkins, Joshua N.; Ansong, Charles; Galagan, James; Kaiser, Shari; Korth, Marcus J.; Law, G. L.; McDermott, Jason E.; Proll, Sean; Rosenberger, Carrie; Schoolnik, Gary; Katze, Michael G.

    2011-02-01

    The 20th century was marked by extraordinary advances in our understanding of microbes and infectious disease, but pandemics remain, food and water borne illnesses are frequent, multi-drug resistant microbes are on the rise, and the needed drugs and vaccines have not been developed. The scientific approaches of the pastincluding the intense focus on individual genes and proteins typical of molecular biologyhave not been sufficient to address these challenges. The first decade of the 21st century has seen remarkable innovations in technology and computational methods. These new tools provide nearly comprehensive views of complex biological systems and can provide a correspondingly deeper understanding of pathogen-host interactions. To take full advantage of these innovations, the National Institute of Allergy and Infectious Diseases recently initiated the Systems Biology Program for Infectious Disease Research. As participants of the Systems Biology Program we think that the time is at hand to redefine the pathogen-host research paradigm.

  10. Severe gas bubble disease in a warmwater fishery in the midwestern united states

    SciTech Connect (OSTI)

    Crunkilton, R.L.; Czarnezki, J.M.; Trial, L.

    1980-11-01

    Supersaturation of the water of the Osage River below the Harry S. Truman Dam resulted in an epidemic of gas bubble disease that caused the largest fish kill in the history of Missouri. This is the first recorded evidence of serious supersaturation in the midwestern U.S. Total gas saturation levels up to 139% killed nearly 500,000 fish in the Osage River and the Lake of the Ozarks during April-May 1978. Nitrogen was the primary gas responsible for gas bubble disease mortalities. Instream cage bioassays defined the zone of lethal supersaturation. Significant mortality occurred in pelagic and near-shore species, deepwater species, and bottom-dwelling species. Susceptibility to disease was related to fish size.

  11. Severe gas bubble disease in a warmwater fishery in the midwestern United States

    SciTech Connect (OSTI)

    Crunkilton, R.L.; Czarnezki, J.M.; Trial, L.

    1980-11-01

    Gas bubble disease below Harry S. Truman Dam, sited on the upper Osage River and spilling into Lake of the Ozarks, caused the largest fish kill on record in Missouri. This is the first recorded evidence of serious supersaturation in the Midwest. Total gas saturation levels up to 139% killed nearly a half million fish in the upper 85 km of the Osage Arm, Lake of the Ozarks, during April to June, 1978 and 1979. Gas supersaturation occurred throughout the 150 km of this main-stem reservoir. Nitrogen was the primary gas responsible for gas bubble disease mortalities. Pelagic and near-shore species suffered the earliest and heaviest mortalities, but fish characteristic of deeper waters were increasingly killed as supersaturation persisted. Instream cage bioassays defined the zone of lethal supersaturation. Significant mortality occurred in bottom-dwelling fish of several species, due to long-term intermittent exposure. Susceptibility to gas bubble disease was related to fish size.

  12. Linkage disequilibrium in the region of the autosomal dominant polycystic kidney disease gene (PKD1)

    SciTech Connect (OSTI)

    Snarey, A. ); Thomas, S.; Harris, P.C. ); Schneider, M.C. ); Pound, S.E.; Wright, A.F. ); Barton, N.; Somlo, S.; Germino, G.G.; Reeders, S.T.

    1994-08-01

    The gene for autosomal dominant polycystic kidney disease (PKD1) is located on chromosome 16p, between the flanking markers D16S84 and D16S125 (26.6 prox). This region is 750 kb long and has been cloned. The authors have looked at the association of 10 polymorphic markers from the region, with the disease and with each other. This was done in a set of Scottish families that had previously shown association with D16S94, a marker proximal to the PKD1 region. They report significant association between two CA repeat markers and the disease but have not found evidence for a single founder haplotype in these families, indicating the presence of several mutations in this population. Their results favor a location of the PKD1 gene in the proximal part of the candidate region. 25 refs., 1 fig., 4 tabs.

  13. Serum Proteome Profiles in Stricturing Crohn’s Disease: A pilot study.

    SciTech Connect (OSTI)

    Townsend, Peter; Zhang, Qibin; Shapiro, Jason; Webb-Robertson, Bobbie-Jo M.; Bramer, Lisa M.; Schepmoes, Athena A.; Weitz, Karl K.; Mallette, Meaghan; Moniz, Heather; Bright, Renee; Merrick, Marjorie; Shah, Samir A.; Sands, Bruce E.; Leleiko, Neal

    2015-08-01

    Background: Crohn’s disease (CD) is a form of inflammatory bowel disease (IBD) with different described behaviors, including stricture. At present, there are no laboratory studies that can differentiate stricturing CD from other phenotypes of IBD. We performed a pilot study to examine differences in the proteome among patients with stricturing Crohn’s disease, non-stricturing Crohn’s disease, and ulcerative colitis (UC). Methods: Serum samples were selected from the Ocean State Crohn’s and Colitis Area Registry (OSCCAR), an established cohort of patients with IBD. Crohn’s disease patients with surgically-resected stricture were matched with similar patients with Crohn’s disease without known stricture, and with UC. Serum samples from each patient were digested and analyzed using liquid chromatography-mass spectrometry to characterize the proteome. Statistical analyses were performed to identify peptides and proteins that can differentiate CD with stricture. Results: Samples from 9 patients in each group (27 total patients) were analyzed. Baseline demographic characteristics were similar among the three groups. We quantified 7668 peptides and 897 proteins for analysis. ROC analysis identified a subset of peptides with an area under the curve greater than 0.9, indicating greater separation potential. Partial least squares discriminant analysis was able to distinguish among the three groups with up to 70% accuracy by peptides, and up to 80% accuracy by proteins. We identified the significantly different proteins and peptides, and determined their function based on previously published literature. Conclusions: The serum of patients with stricturing CD, non-stricturing CD, and UC are distinguishable via proteomic analysis. Some of the proteins that differentiate the stricturing phenotype have been implicated in complement activation, fibrinolytic pathways, and lymphocyte adhesion.

  14. Gas Bubble Disease Monitoring and Research of Juvenile Salmonids : Annual Report 1996.

    SciTech Connect (OSTI)

    Maule, Alec G.; Beeman, John W.; Hans, Karen M.; Mesa, M.G.; Haner, P.; Warren, J.J.

    1997-10-01

    This document describes the project activities 1996--1997 contract year. This report is composed of three chapters which contain data and analyses of the three main elements of the project: field research to determine the vertical distribution of migrating juvenile salmonids, monitoring of juvenile migrants at dams on the Snake and Columbia rivers, and laboratory experiments to describe the progression of gas bubble disease signs leading to mortality. The major findings described in this report are: A miniature pressure-sensitive radio transmitter was found to be accurate and precise and, after compensation for water temperature, can be used to determine the depth of tagged-fish to within 0.32 m of the true depth (Chapter 1). Preliminary data from very few fish suggest that depth protects migrating juvenile steelhead from total dissolved gas supersaturation (Chapter 1). As in 1995, few fish had any signs of gas bubble disease, but it appeared that prevalence and severity increased as fish migrated downstream and in response to changing gas supersaturation (Chapter 2). It appeared to gas bubble disease was not a threat to migrating juvenile salmonids when total dissolved gas supersaturation was < 120% (Chapter 2). Laboratory studies suggest that external examinations are appropriate for determining the severity of gas bubble disease in juvenile salmonids (Chapter 3). The authors developed a new method for examining gill arches for intravascular bubbles by clamping the ventral aorta to reduce bleeding when arches were removed (Chapter 3). Despite an outbreak of bacterial kidney disease in the experimental fish, the data indicate that gas bubble disease is a progressive trauma that can be monitored (Chapter 3).

  15. Geek-Up[04.01.2011]: A Discovery to Fight Cancer and Other Diseases |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 4.01.2011]: A Discovery to Fight Cancer and Other Diseases Geek-Up[04.01.2011]: A Discovery to Fight Cancer and Other Diseases April 1, 2011 - 5:52pm Addthis Two structures of the Mre11-Rad50 complex were solved independently and overlaid, further revealing a flexible hinge in Rad50 near the Mre11 binding site | Courtesy of Lawrence Berkeley National Laboratory Two structures of the Mre11-Rad50 complex were solved independently and overlaid, further revealing a flexible

  16. Suppression of somatic expansion delays the onset of pathophysiology in a mouse model of Huntington’s Disease

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Budworth, Helen; Harris, Faye R.; Williams, Paul; Lee, Do Yup; Holt, Amy; Pahnke, Jens; Szczesny, Bartosz; Acevedo-Torres, Karina; Ayala-Peña, Sylvette; McMurray, Cynthia T.; et al

    2015-08-06

    Huntington’s Disease (HD) is caused by inheritance of a single disease-length allele harboring an expanded CAG repeat, which continues to expand in somatic tissues with age. The inherited disease allele expresses a toxic protein, and whether further somatic expansion adds to toxicity is unknown. We have created an HD mouse model that resolves the effects of the inherited and somatic expansions. We show here that suppressing somatic expansion substantially delays the onset of disease in littermates that inherit the same disease-length allele. Furthermore, a pharmacological inhibitor, XJB-5-131, inhibits the lengthening of the repeat tracks, and correlates with rescue of motormore » decline in these animals. The results provide evidence that pharmacological approaches to offset disease progression are possible.« less

  17. Suppression of somatic expansion delays the onset of pathophysiology in a mouse model of Huntington’s Disease

    SciTech Connect (OSTI)

    Budworth, Helen; Harris, Faye R.; Williams, Paul; Lee, Do Yup; Holt, Amy; Pahnke, Jens; Szczesny, Bartosz; Acevedo-Torres, Karina; Ayala-Peña, Sylvette; McMurray, Cynthia T.; McKinnon, Peter

    2015-08-06

    Huntington’s Disease (HD) is caused by inheritance of a single disease-length allele harboring an expanded CAG repeat, which continues to expand in somatic tissues with age. The inherited disease allele expresses a toxic protein, and whether further somatic expansion adds to toxicity is unknown. We have created an HD mouse model that resolves the effects of the inherited and somatic expansions. We show here that suppressing somatic expansion substantially delays the onset of disease in littermates that inherit the same disease-length allele. Furthermore, a pharmacological inhibitor, XJB-5-131, inhibits the lengthening of the repeat tracks, and correlates with rescue of motor decline in these animals. The results provide evidence that pharmacological approaches to offset disease progression are possible.

  18. ARSCL Cloud Statistics - A Value-Added Product

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    data from active remote sensors to produce an objective determination of cloud location, radar reflectivity, vertical velocity, and Doppler spectral width. Information about the...

  19. Adding a MOAB Geometry Interface to SHARP Structural Mechanics

    SciTech Connect (OSTI)

    Ferencz, R M; Hodge, N E

    2012-05-28

    The authors briefly summarize the development of, and test experience with, an initial data interface between the structural mechanics code Diablo and the SHARP reactor simulation system data hub MOAB. That interface has been exercised both to write MOAB databases from Diablo, and then also to use such a database to read in part of a simulation definition for a subsequent Diablo execution. All enhancements are integrated into the central Diablo source repository. The SHARP software system for advanced simulation of nuclear reactors and power plant systems is sponsored by DOE's Nuclear Energy Advanced Modeling and Simulation (NEAMS) program. SHARP has been architected as a federation of single-physics simulation tools to permit flexibility in programming langugages and leveraging of past and on-going investments. Solution of multi-physics problems will be coordinated by, and data passed through, a central 'hub'. SHARP's hub implementation is utilizing MOAB: a Mesh-Oriented datABase. This same data hub approach is also intended to enable multi-resolution simulations, e.g, lower-dimension plant-scale simulations can be informed by high-fidelity 3D models of particular critical components.

  20. New methods for identifying value added by a regional climate...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Brian Grabowski at media@anl.gov or (630) 252-1232. Connect Find an Argonne expert by subject. Follow Argonne on Twitter, Facebook, Google+ and LinkedIn. For inquiries on...

  1. USDA Webinar: Value-Added Producer Grants for Tribal Entities

    Broader source: Energy.gov [DOE]

    Tuesday, January 149:00 – 10:30 AM Alaska Standard Time10:00 – 11:30 AM Pacific Standard Time11:00 AM-12:30 PM Mountain Standard Time12:00 PM – 1:30 PM Central Standard Time1:00 PM-2:30 PM Eastern...

  2. ARM Evaluation Product : Droplet Number Concentration Value-Added...

    Office of Scientific and Technical Information (OSTI)

    They show that the magnitude of the aerosol-cloud interactions (ACI) varies with a range of factors, including the relative value of the cloud liquid water path (LWP), the aerosol ...

  3. Report of ad hoc OTEC cold water pipe committee

    SciTech Connect (OSTI)

    Barr, R.; Giannotti, J.; Deuchler, W.; Scotti, R.; Stadter, J.; Walsh, J. P.; Weiss, R.

    1980-02-01

    Now that the design work on the pilot plant is scheduled to start in the near future, DOE has considered it essential that an overall look be taken at the cold water pipe design process. The VSE Corporation, in its role as a support contractor to DOE, was tasked to organize a small study group to answer the question, Where do we stand on the verification of the computer models of the cold water pipe response by experimental measurements. The committee has studied all the available results of the cold water pipe development program. This report summarizes those results. The development and present capabilities of the computer programs used to calculate the response of a cold water pipe attached to a platform under known at-sea conditions are discussed. The various cold water pipe designs that have been done using the computer programs are summarized. The experiments that have been conducted up to the present time to measure the response of cold water pipes at-sea and in experimental tanks are described. The results of these experiments are presented. The experimental results are compared with the predictions made with the analytical computer programs. Conclusions drawn as a result of this analysis are presented and some recommendations are made. (WHK)

  4. Issues in Value-Added Products from Biomass

    SciTech Connect (OSTI)

    Elliott, Douglas C.

    2001-11-01

    While biomass conversion to energy products has been the primary focus of most development efforts over the past 30 years, process development for chemical products is an area of increasing effort more recently. Although the fuels market is likely to allow the largest impact for renewable resources in the world economy, chemical products can also be more than just niche market applications. However, the specific chemical processing required for refined chemical products requires improved chemical handling methods for separations and purifications, as well as improved catalyst systems. Development of these unit operations has lagged behind the process research focused on the finished products. This paper will describe some of the critical processing issues that need to be addressed to allow biomass feedstocks to make a real impact in the chemicals market. The paper will also describe some of the process research which has been performed or is now underway in our laboratory and others'. Areas to be discussed include biomass component separation, catalyst development for aqueous processing, and trace component effects in catalytic processing of biomass feedstocks.

  5. Microsoft Word - FDI A&D 2007.doc

    U.S. Energy Information Administration (EIA) Indexed Site

    XXI (Bermuda) - oil & gas assets oil & gas production Pogo Producing 410 Repsol YPF (Spain) Repsol Energy North America oil & gas assets (28 percent of Genghis Khan) oil & gas...

  6. ARM KAZR-ARSCL Value Added Product (Dataset) | Data Explorer

    Office of Scientific and Technical Information (OSTI)

    Country of Publication: United States Availability: ORNL Language: English Subject: 54 Environmental Sciences ARM, KAZR, ARSCL,KAZR-ARSCL Dataset File size NAView Dataset ...

  7. Section 180(c) Ad Hoc Working Group | Department of Energy

    Office of Environmental Management (EM)

    Continuing Appropriations Act | Department of Energy 29 of the Consolidated Security, Disaster Assistance, and Continuing Appropriations Act Section 129 of the Consolidated Security, Disaster Assistance, and Continuing Appropriations Act Section 129 of the Consolidated Security, Disaster Assistance, and Continuing Appropriations Act (480.1 KB) More Documents & Publications Energy Policy Act of 2005 NATIONAL DEFENSE AUTHORIZATION ACT FOR FISCAL YEAR 2000 Energy Policy Act of 2005

    512

  8. Security Communications Protocol Ad Hoc Working Group | Department of

    Office of Environmental Management (EM)

    Renewable Energy (EERE) | Department of Energy Securing America's Clean Energy Future (Fact Sheet), Energy Efficiency & Renewable Energy (EERE) Securing America's Clean Energy Future (Fact Sheet), Energy Efficiency & Renewable Energy (EERE) This two-page fact sheet provides an overview of the activities and programs in DOE's Office of Energy Efficiency and Renewable Energy. 51111.pdf (656.28 KB) More Documents & Publications Office of the Biomass Program Educational Opportunities

  9. Aerosol Optical Depth Value-Added Product Report

    SciTech Connect (OSTI)

    Koontz, A; Hodges, G; Barnard, J; Flynn, C; Michalsky, J

    2013-03-17

    This document describes the process applied to retrieve aerosol optical depth (AOD) from multifilter rotating shadowband radiometers (MFRSR) and normal incidence multifilter radiometers (NIMFR) operated at the ARM Climate Research Facility’s ground-based facilities.

  10. WIPP Exhibit: Message to 12,000 A.D.

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    place is not a place of honor. No highly esteemed deed is commemorated here. Nothing valued is here. This place is a message and part of a system of messages. Pay attention to it! Sending this message was important to us. We considered ourselves to be a powerful culture. Excerpts from Expert Judgment on Markers to Deter Inadvertent Human Intrusion into the Waste Isolation Pilot Plant, Sandia National Laboratories report SAND92-1382 / UC-721, p. F-49 Team A: * Dieter G. Ast (Cornell University) *

  11. Organic Aerosol Component (OACOMP) Value-Added Product Report...

    Office of Scientific and Technical Information (OSTI)

    The key outputs of OACOMP are the concentration time series and the mass spectra of OA factors that are associated with distinct sources, formation and evolution processes, and ...

  12. AD&D insurance offers three coverage options

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Only: covers only you Employee + Child(ren) or Modified Family: covers you and your children* Employee + Family: covers you, your spousedomestic partner and your children* *The...

  13. Expert Meeting Report: Key Innovations for Adding Energy Efficiency...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    or implied, or assumes any legal liability or responsibility ... orders@ntis.fedworld.gov online ordering: http:... upgrades and what training is necessary to use the ...

  14. Droplet Number Concentration Value-Added Product (Technical Report...

    Office of Scientific and Technical Information (OSTI)

    LWP is from the adiabatic case. quality control (QC) flags (qcdropnumberconc), an ... Sponsoring Org: USDOE Office of Science (SC), Biological and Environmental Research (BER) ...

  15. Best Estimate Radiation Flux Value-Added Procedure: Algorithm...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the U.S. Department of Energy, Office of Energy Research, Office of Health and ......... 1 3. Configuration Files......

  16. Adding Data Management Services to Parallel File Systems

    SciTech Connect (OSTI)

    Brandt, Scott

    2015-03-04

    The objective of this project, called DAMASC for “Data Management in Scientific Computing”, is to coalesce data management with parallel file system management to present a declarative interface to scientists for managing, querying, and analyzing extremely large data sets efficiently and predictably. Managing extremely large data sets is a key challenge of exascale computing. The overhead, energy, and cost of moving massive volumes of data demand designs where computation is close to storage. In current architectures, compute/analysis clusters access data in a physically separate parallel file system and largely leave it scientist to reduce data movement. Over the past decades the high-end computing community has adopted middleware with multiple layers of abstractions and specialized file formats such as NetCDF-4 and HDF5. These abstractions provide a limited set of high-level data processing functions, but have inherent functionality and performance limitations: middleware that provides access to the highly structured contents of scientific data files stored in the (unstructured) file systems can only optimize to the extent that file system interfaces permit; the highly structured formats of these files often impedes native file system performance optimizations. We are developing Damasc, an enhanced high-performance file system with native rich data management services. Damasc will enable efficient queries and updates over files stored in their native byte-stream format while retaining the inherent performance of file system data storage via declarative queries and updates over views of underlying files. Damasc has four key benefits for the development of data-intensive scientific code: (1) applications can use important data-management services, such as declarative queries, views, and provenance tracking, that are currently available only within database systems; (2) the use of these services becomes easier, as they are provided within a familiar file-based ecosystem; (3) common optimizations, e.g., indexing and caching, are readily supported across several file formats, avoiding effort duplication; and (4) performance improves significantly, as data processing is integrated more tightly with data storage. Our key contributions are: SciHadoop which explores changes to MapReduce assumption by taking advantage of semantics of structured data while preserving MapReduce’s failure and resource management; DataMods which extends common abstractions of parallel file systems so they become programmable such that they can be extended to natively support a variety of data models and can be hooked into emerging distributed runtimes such as Stanford’s Legion; and Miso which combines Hadoop and relational data warehousing to minimize time to insight, taking into account the overhead of ingesting data into data warehousing.

  17. NREL: Energy Systems Integration - NREL Assesses Costs of Adding...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    to Existing Power Systems September 29, 2015 Much effort has been made to assess the costs associated with integrating variable generation (VG) such as wind and solar into an...

  18. ARM Climate Research Facility Quarterly Value-Added Product Report...

    Office of Scientific and Technical Information (OSTI)

    (VAP) implemented by the Atmospheric Radiation Measurement Climate Research Facility. ... approved, (4) other work that leads to a VAP, and (5) top requested VAPs from the archive. ...

  19. Station-based Surface Data Value-Added Product

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... It is a twin data product of the ARMBE 2-Dimensional gridded (ARMBE2DGRID) data set. Unlike the ARMBE2DGRID data set, ARMBESTNS data are reported at the original site locations and ...

  20. Adding Fault Tolerance to NPB Benchmarks Using ULFM

    SciTech Connect (OSTI)

    Parchman, Zachary W; Vallee, Geoffroy R; Naughton III, Thomas J; Engelmann, Christian; Bernholdt, David E; Scott, Stephen L

    2016-01-01

    In the world of high-performance computing, fault tolerance and application resilience are becoming some of the primary concerns because of increasing hardware failures and memory corruptions. While the research community has been investigating various options, from system-level solutions to application-level solutions, standards such as the Message Passing Interface (MPI) are also starting to include such capabilities. The current proposal for MPI fault tolerant is centered around the User-Level Failure Mitigation (ULFM) concept, which provides means for fault detection and recovery of the MPI layer. This approach does not address application-level recovery, which is currently left to application developers. In this work, we present a mod- ification of some of the benchmarks of the NAS parallel benchmark (NPB) to include support of the ULFM capabilities as well as application-level strategies and mechanisms for application-level failure recovery. As such, we present: (i) an application-level library to checkpoint and restore data, (ii) extensions of NPB benchmarks for fault tolerance based on different strategies, (iii) a fault injection tool, and (iv) some preliminary results that show the impact of such fault tolerant strategies on the application execution.

  1. Doppler Lidar Vertical Velocity Statistics Value-Added Product...

    Office of Scientific and Technical Information (OSTI)

    Citation Details In-Document Search Title: Doppler Lidar Vertical Velocity Statistics ... Facility operates coherent Doppler lidar systems at several sites around the globe. ...

  2. Ion beam collimating grid to reduce added defects

    DOE Patents [OSTI]

    Lindquist, Walter B. (Oakland, CA); Kearney, Patrick A. (Livermore, CA)

    2003-01-01

    A collimating grid for an ion source located after the exit grid. The collimating grid collimates the ion beamlets and disallows beam spread and limits the beam divergence during transients and steady state operation. The additional exit or collimating grid prevents beam divergence during turn-on and turn-off and prevents ions from hitting the periphery of the target where there is re-deposited material or from missing the target and hitting the wall of the vessel where there is deposited material, thereby preventing defects from being deposited on a substrate to be coated. Thus, the addition of a collimating grid to an ion source ensures that the ion beam will hit and be confined to a specific target area.

  3. Addendum Added to Innovative Demonstration of Geothermal Energy Production FOA

    Broader source: Energy.gov [DOE]

    The U.S. Department of Energy’s (DOE) Geothermal Technologies Program (GTP) has released a Funding Opportunity Announcement (FOA) that seeks innovative demonstration of energy production from non-conventional geothermal resources.

  4. Cloud Condensation Nuclei Profile Value-Added Product (Technical...

    Office of Scientific and Technical Information (OSTI)

    Authors: McFarlane, S ; Sivaraman, C ; Ghan, S Publication Date: 2012-10-08 OSTI Identifier: 1052588 Report Number(s): DOESC-ARMTR-103 PNNL-21877 DOE Contract Number: ...

  5. Aerosol Optical Depth Value-Added Product Report (Technical Report...

    Office of Scientific and Technical Information (OSTI)

    Authors: Koontz, A ; Hodges, G ; Barnard, J ; Flynn, C ; Michalsky, J Publication Date: 2013-03-17 OSTI Identifier: 1092419 Report Number(s): DOESC-ARMTR-129 PNNL-22260 DOE ...

  6. Raman Lidar Profiles-Temperature (RLPROFTEMP) Value-Added Product...

    Office of Scientific and Technical Information (OSTI)

    Authors: Newsom, RK ; Sivaraman, C ; McFarlane, SA Publication Date: 2012-10-31 OSTI Identifier: 1053989 Report Number(s): DOESC-ARMTR-120 PNNL-21965 DOE Contract Number: ...

  7. Interpolated Sounding Value-Added Product (Technical Report)...

    Office of Scientific and Technical Information (OSTI)

    OSTI Identifier: 1226794 Report Number(s): DOESC-ARMTR-124 DOE Contract Number: ... Full Text preview image File size NAView Full Text View Full Text DOI: 10.21721226794

  8. PEAC{trademark} value-added project. Final report

    SciTech Connect (OSTI)

    Sheesley, D.

    1997-09-01

    Chemical spill emergencies are exacerbated by a lack of information at the scene with which to quickly assess the situation and determine the appropriate protective action. WRI has concluded that the most successful emergency tools (models) for predicting toxic cloud behavior are those that use field test data as part of their development. After analyzing more than 100 real-world chemical spill accidents and field test releases, WRI has also concluded that information from dense gas/toxic models must be communicated in a fast, simple way to emergency responders or it will not be used. In an effort to address this technology gap, WRI has developed PEAC{trademark} (Palmtop Emergency Action for Chemicals). PEAC is an innovative tool that combines hardware, software, data bases developed by WRI and other organizations, and a unique approach to modeling protective action distances tested with actual field data. PEAC brings modeling data and other reference sources together in a single piece of portable equipment to help emergency responders evaluate a chemical spill incident and quickly determine protective action distances.

  9. Method for adding nodes to a quantum key distribution system

    DOE Patents [OSTI]

    Grice, Warren P

    2015-02-24

    An improved quantum key distribution (QKD) system and method are provided. The system and method introduce new clients at intermediate points along a quantum channel, where any two clients can establish a secret key without the need for a secret meeting between the clients. The new clients perform operations on photons as they pass through nodes in the quantum channel, and participate in a non-secret protocol that is amended to include the new clients. The system and method significantly increase the number of clients that can be supported by a conventional QKD system, with only a modest increase in cost. The system and method are compatible with a variety of QKD schemes, including polarization, time-bin, continuous variable and entanglement QKD.

  10. Catalyst added to Claus furnace reduces sulfur losses

    SciTech Connect (OSTI)

    Luinstra, E.A.; d'Haene, P.E. (Shell Canada Ltd., Toronto, ON (Canada). Oakville Research Centre)

    1989-07-01

    Several substances effectively catalyze the reduction of carbon disulfide in Claus gas streams at Claus reaction furnace conditions (about 1,000{sup 0}C). Some conversion of carbonyl sulfide also occurs. Carbon disulfide and carbonyl sulfide as well-known problem compounds that reduce sulfur recovery efficiency in many sulfur recovery plants. Installation of a suitable catalytic material in the reaction furnace promises significant improvement of Claus plant efficiency, and prolonged life of the catalytic converters. Almost every Claus sulfur recovery plant makes some carbon disulfide (CS/sub 2/) and carbonyl sulfide (COS) in the reaction furnace, and in many of these plants, these compounds constitute a significant problem. CS/sub 2/ and COS often comprise more than 50% of sulfur losses in the tail gas. This article reexamines the issue of CS/sub 2/ and COS in the Claus plant. The relative importance of these two troublesome components is explored with data accumulated from Shell Canada Claus plants. The authors discuss which factors tend to produce these components. Then a method for reducing CS/sub 2/ and COS virtually at the source will be introduced.

  11. How Energy Efficiency is Adding Jobs in St. Paul, Minnesota

    Broader source: Energy.gov [DOE]

    Saint Paul, Minnesota is using an energy efficiency grant to provide commercial retrofits that will allow a local produce distribution company to dramatically reduce its energy costs and add dozens...

  12. Interpolated Sounding and Gridded Sounding Value-Added Products

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... or sondewnpn.b1 vwind vwind T Toto and M Jensen, March 2016, DOESC-ARM-TR-183 4 Note that GRIDDEDSONE is not provided by ARM as an available data stream; however, the data ...

  13. ARM Evaluation Product : Droplet Number Concentration Value-Added Product

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Riihimaki, Laura

    Cloud droplet number concentration is an important factor in understanding aerosol-cloud interactions. As aerosol concentration increases, it is expected that droplet number concentration, Nd, will increase and droplet size decrease, for a given liquid water path (Twomey 1977), which will greatly affect cloud albedo as smaller droplets reflect more shortwave radiation. However, the magnitude and variability of these processes under different environmental conditions is still uncertain. McComiskey et al. (2009) have implemented a method, based on Boers and Mitchell (1994), for calculating Nd from ground-based remote sensing measurements of optical depth and liquid water path. They show that the magnitude of the aerosol-cloud interactions (ACI) varies with a range of factors, including the relative value of the cloud liquid water path (LWP), the aerosol size distribution, and the cloud updraft velocity. Estimates of Nd under a range of cloud types and conditions and at a variety of sites are needed to further quantify the impacts of aerosol cloud interactions.

  14. Task 4.9 -- Value-added products from syngas

    SciTech Connect (OSTI)

    Olson, E.S.; Sharma, R.K.

    1997-02-01

    The work on advanced fuel forms in 1996 focused on the synthesis of higher alcohols from mixtures of hydrogen and carbon dioxide (syngas) from coal gasification. The conversion of coal gasification products to commercially valuable alcohols will provide an important new market for current and future gasification plants. Initial work in this project utilized a novel molybdenum sulfide catalyst previously shown to be active for hydrodesulfurization reactions of coal liquids. The support for the active metal sulfide is a layered mixed oxide (hydrotalcite) capable of interaction with the metal sites for catalysis of carbon monoxide reductions. These catalysts have a high surface area, are highly porous, and have basic and acidic functionality. A pressurized fixed-bed flow-through reactor was constructed, and the MoS{sub 2} catalysts were tested with syngas under a variety of conditions. Unfortunately, the catalysts, even with higher molybdenum loading and addition of promoters, failed to give alcohol products. A batch reactor test of the catalyst was also conducted, but did not produce alcohol products. Group 8 metals have been used previously in catalysts for syngas reactions. Ruthenium and rhodium catalysts were prepared by impregnation of a hydrotalcite support. Tests with these catalysts in flow-through reactors also did not produce the desired alcohol products. The formation of higher alcohols from smaller ones, such as methanol and ethanol, could be commercially important if high selectivity could be achieved. The methanol and ethanol would be derived from syngas and fermentation, respectively. Based on previous work in other laboratories, it was hypothesized that the hydrotalcite supported MoS{sub 2} or Ru or Rh catalysts could catalyze the formation of butyl alcohols. Although the desired 1-butanol was obtained in batch reactions with the promoted Ru catalyst, the reaction was not as selective as desired. Product suitable for a lower-vapor-pressure gasoline oxygenate additive was obtained, but it may not be economical to market such products in competition with methyl tertiary-butyl-ether. Flow-through catalytic bed reactions were not successful.

  15. Value-Added Attributes of the QA Requirements

    Broader source: Energy.gov [DOE]

    1. Senior Management Ownership Senior management must take full ownership of the quality assurance program. These managers should establish policies and objectives focused on achieving the organization's mission while improving the quality of the organization's products and services. They must create an environment that promotes quality and the improvement of quality throughout the entire organization.

  16. ARM Evaluation Product : Droplet Number Concentration Value-Added Product

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Riihimaki, Laura

    2014-05-15

    Cloud droplet number concentration is an important factor in understanding aerosol-cloud interactions. As aerosol concentration increases, it is expected that droplet number concentration, Nd, will increase and droplet size decrease, for a given liquid water path (Twomey 1977), which will greatly affect cloud albedo as smaller droplets reflect more shortwave radiation. However, the magnitude and variability of these processes under different environmental conditions is still uncertain. McComiskey et al. (2009) have implemented a method, based on Boers and Mitchell (1994), for calculating Nd from ground-based remote sensing measurements of optical depth and liquid water path. They show that the magnitude of the aerosol-cloud interactions (ACI) varies with a range of factors, including the relative value of the cloud liquid water path (LWP), the aerosol size distribution, and the cloud updraft velocity. Estimates of Nd under a range of cloud types and conditions and at a variety of sites are needed to further quantify the impacts of aerosol cloud interactions.

  17. AdS/CFT and Large-N Volume Independence

    SciTech Connect (OSTI)

    Poppitz, Erich; Unsal, Mithat; /SLAC /Stanford U., Phys. Dept.

    2010-08-26

    We study the Eguchi-Kawai reduction in the strong-coupling domain of gauge theories via the gravity dual of N=4 super-Yang-Mills on R{sup 3} x S{sup 1}. We show that D-branes geometrize volume independence in the center-symmetric vacuum and give supergravity predictions for the range of validity of reduced large-N models at strong coupling.

  18. Value Added Energy Information Systems VAEIS | Open Energy Information

    Open Energy Info (EERE)

    Solar, Wind energy Product: Provides turn-key monitoring systems for the performance of solar, wind, fuel cell and other distributed generation installations. Coordinates:...

  19. ARM Value-Added Cloud Products: Description and Status

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    This VAP combines the data from the millimeter cloud radar (MMCR), micropulse lidar (MPL), laser ceilometer, microwave radiometer (MWR), and surface measurements. It produces a ...

  20. How Energy Efficiency is Adding Jobs in St. Paul, Minnesota

    ScienceCinema (OSTI)

    Hannigan, Jim; Coleman, Chris; Oliver, LeAnn; Jambois, Louis

    2013-05-29

    Saint Paul, Minnesota is using an energy efficiency grant to provide commercial retrofits that will allow a local produce distribution company to dramatically reduce its energy costs and add dozens of new workers.

  1. Atmospheric Radiation Measurement (ARM) Value-Added Data Products...

    Office of Scientific and Technical Information (OSTI)

    of the quality of the input data. taken from http:www.arm.govdatavapsall.php One of the ARM data centers, the External Data Center or XDC at Brookhaven National Laboratory, ...

  2. Organic Aerosol Component (OACOMP) Value-Added Product (Technical...

    Office of Scientific and Technical Information (OSTI)

    But atmospheric OA is poorly characterized and its life cycle insufficiently represented in models. As a result, current models are unable to simulate OA concentrations and ...

  3. Ad Lucem: Modeling of Market Transformation Pathways Workshop...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    energy technology market dynamics. 2. Modeling solar energy technology evolution ... market, as well as institutional behavioral influences in the context of U.S. society. ...

  4. New Gapminder Visualizations Added! | OpenEI Community

    Open Energy Info (EERE)

    You must login in order to post into this group. Latest blog post comments Mike o spectacular dataset Posted: 9 Aug 2015 - 14:46 by: Mike o Calum Residential idf Files Posted:...

  5. Hydropower Upgrades to Yield Added Generation at Average Costs...

    Office of Environmental Management (EM)

    ... Air pollution would be reduced in a non-attainment air basin as the debris has been previously burned for removal. Following negotiation of final funding amounts, projects are ...

  6. Small particle aerosol inoculation of cowpox Brighton Red in rhesus monkeys results in a severe respiratory disease

    SciTech Connect (OSTI)

    Johnson, Reed F.; Hammoud, Dima A.; Lackemeyer, Matthew G.; Yellayi, Srikanth; Solomon, Jeffrey; Bohannon, Jordan K.; Janosko, Krisztina B.; Jett, Catherine; Cooper, Kurt; Blaney, Joseph E.; Jahrling, Peter B.

    2015-07-15

    Cowpox virus (CPXV) inoculation of nonhuman primates (NHPs) has been suggested as an alternate model for smallpox (Kramski et al., 2010, PLoS One, 5, e10412). Previously, we have demonstrated that intrabronchial inoculation of CPXV-Brighton Red (CPXV-BR) into cynomolgus monkeys resulted in a disease that shared many similarities to smallpox; however, severe respiratory tract disease was observed (Smith et al., 2011, J. Gen. Virol.). Here we describe the course of disease after small particle aerosol exposure of rhesus monkeys using computed tomography (CT) to monitor respiratory disease progression. Subjects developed a severe respiratory disease that was uniformly lethal at 5.7 log{sub 10} PFU of CPXV-BR. CT indicated changes in lung architecture that correlated with changes in peripheral blood monocytes and peripheral oxygen saturation. While the small particle aerosol inoculation route does not accurately mimic human smallpox, the data suggest that CT can be used as a tool to monitor real-time disease progression for evaluation of animal models for human diseases. - Highlights: • Small particle aerosol exposure of rhesus results in a severe respiratory disease. • CT findings correlated with peripheral oxygen saturation and monocyte increases. • Virus dissemination was limited and mainly confined to the respiratory tract. • CT provides insight into pathogenesis to aid development of animal models of disease.

  7. Higher coronary heart disease and heart attack morbidity in Appalachian coal mining regions

    SciTech Connect (OSTI)

    Hendryx, M.; Zullig, K.J.

    2009-11-15

    This study analyzes the U.S. 2006 Behavioral Risk Factor Surveillance System survey data (N = 235,783) to test whether self-reported cardiovascular disease rates are higher in Appalachian coal mining counties compared to other counties after control for other risks. Dependent variables include self-reported measures of ever (1) being diagnosed with cardiovascular disease (CVD) or with a specific form of CVD including (2) stroke, (3) heart attack, or (4) angina or coronary heart disease (CHD). Independent variables included coal mining, smoking, BMI, drinking, physician supply, diabetes co-morbidity, age, race/ethnicity, education, income, and others. SUDAAN Multilog models were estimated, and odds ratios tested for coal mining effects. After control for covariates, people in Appalachian coal mining areas reported significantly higher risk of CVD (OR = 1.22, 95% CI = 1.14-1.30), angina or CHO (OR = 1.29, 95% C1 = 1.19-1.39) and heart attack (OR = 1.19, 95% C1 = 1.10-1.30). Effects were present for both men and women. Cardiovascular diseases have been linked to both air and water contamination in ways consistent with toxicants found in coal and coal processing. Future research is indicated to assess air and water quality in coal mining communities in Appalachia, with corresponding environmental programs and standards established as indicated.

  8. Implementation Guide for use with 10 CFR Part 850, Chronic Beryllium Disease Prevention Program

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2001-01-04

    The Department of Energy (DOE) has established regulatory requirements for the Chronic Beryllium Disease Prevention Program (CBDPP) in Title 10 of the Code of Federal Regulations (CFR), Part 850 (10 CFR 850) [64 Federal Register (FR) 68854]. Supersedes DOE G 440.1-7. Certified 9-23-10.

  9. Genomics-enabled sensor platform for rapid detection of viruses related to disease outbreak.

    SciTech Connect (OSTI)

    Brozik, Susan Marie; Manginell, Ronald Paul; Moorman, Matthew Wallace; Xiao, Xiaoyin; Edwards, Thayne L.; Anderson, John Moses; Pfeifer, Kent Bryant; Branch, Darren W.; Wheeler, David Roger; Polsky, Ronen; Lopez, DeAnna M.; Ebel, Gregory D.; Prasad, Abhishek N.; Brozik, James A.; Rudolph, Angela R.; Wong, Lillian P.

    2013-09-01

    Bioweapons and emerging infectious diseases pose growing threats to our national security. Both natural disease outbreak and outbreaks due to a bioterrorist attack are a challenge to detect, taking days after the outbreak to identify since most outbreaks are only recognized through reportable diseases by health departments and reports of unusual diseases by clinicians. In recent decades, arthropod-borne viruses (arboviruses) have emerged as some of the most significant threats to human health. They emerge, often unexpectedly, from cryptic transmission foci causing localized outbreaks that can rapidly spread to multiple continents due to increased human travel and trade. Currently, diagnosis of acute infections requires amplification of viral nucleic acids, which can be costly, highly specific, technically challenging and time consuming. No diagnostic devices suitable for use at the bedside or in an outbreak setting currently exist. The original goals of this project were to 1) develop two highly sensitive and specific diagnostic assays for detecting RNA from a wide range of arboviruses; one based on an electrochemical approach and the other a fluorescent based assay and 2) develop prototype microfluidic diagnostic platforms for preclinical and field testing that utilize the assays developed in goal 1. We generated and characterized suitable primers for West Nile Virus RNA detection. Both optical and electrochemical transduction technologies were developed for DNA-RNA hybridization detection and were implemented in microfluidic diagnostic sensing platforms that were developed in this project.

  10. Black holes in a box: Toward the numerical evolution of black holes in AdS space-times

    SciTech Connect (OSTI)

    Witek, Helvi; Nerozzi, Andrea; Cardoso, Vitor; Herdeiro, Carlos; Sperhake, Ulrich; Zilhao, Miguel

    2010-11-15

    The evolution of black holes in ''confining boxes'' is interesting for a number of reasons, particularly because it mimics the global structure of anti-de Sitter geometries. These are nonglobally hyperbolic space-times and the Cauchy problem may only be well defined if the initial data are supplemented by boundary conditions at the timelike conformal boundary. Here, we explore the active role that boundary conditions play in the evolution of a bulk black hole system, by imprisoning a black hole binary in a box with mirrorlike boundary conditions. We are able to follow the post-merger dynamics for up to two reflections off the boundary of the gravitational radiation produced in the merger. We estimate that about 15% of the radiation energy is absorbed by the black hole per interaction, whereas transfer of angular momentum from the radiation to the black hole is observed only in the first interaction. We discuss the possible role of superradiant scattering for this result. Unlike the studies with outgoing boundary conditions, both of the Newman-Penrose scalars {Psi}{sub 4} and {Psi}{sub 0} are nontrivial in our setup, and we show that the numerical data verifies the expected relations between them.

  11. Severity of liver disease affects HCV kinetics in patients treated with intravenous silibinin monotherapy

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Canini, Laetitia; DebRoy, Swati; Mariño, Zoe; Conway, Jessica M.; Crespo, Gonzalo; Navasa, Miquel; D’Amato, Massimo; Ferenci, Peter; Cotler, Scott J.; Forns, Xavier; et al

    2014-06-10

    HCV kinetic analysis and modeling during antiviral therapy have not been performed in decompensated cirrhotic patients awaiting liver transplantation. Here, viral and host parameters were compared in patients treated with daily intravenous silibinin (SIL) monotherapy for 7 days according to the severity of their liver disease. Data were obtained from 25 patients, 12 non-cirrhotic, 8 with compensated cirrhosis and 5 with decompensated cirrhosis. The standard-biphasic model with time-varying SIL effectiveness (from 0 to εmax) was fit to viral kinetic data. Our results show that baseline viral load and age were significantly associated with the severity of liver disease (p<0.0001). Amore » biphasic viral decline was observed in most patients with a higher first phase decline patients with less severe liver disease. The maximal effectiveness, εmax, was significantly (p≤0.032) associated with increasing severity of liver disease (εmax[s.e.]=0.86[0.05], εmax=0.69[0.06] and εmax=0.59[0.1]). The 2nd phase decline slope was not significantly different among groups (mean 1.88±0.15 log10IU/ml/wk, p=0.75) as was the rate of change of SIL effectiveness (k=2.12/day[standard error, SE=0.18/day]). HCV-infected cell loss rate (δ[SE]=0.62/day[0.05/day]) was high and similar among groups. We conclude that the high loss rate of HCV-infected cells suggests that sufficient dose and duration of SIL might achieve viral suppression in advanced liver disease.« less

  12. TU-C-12A-12: Differentiating Bone Lesions and Degenerative Joint Disease in NaF PET/CT Scans Using Machine Learning

    SciTech Connect (OSTI)

    Perk, T; Bradshaw, T; Muzahir, S; Jeraj, R; Meyer, E

    2014-06-15

    Purpose: [F-18]NaF PET can be used to image bone metastases; however, tracer uptake in degenerative joint disease (DJD) often appears similar to metastases. This study aims to develop and compare different machine learning algorithms to automatically identify regions of [F-18]NaF scans that correspond to DJD. Methods: 10 metastatic prostate cancer patients received whole body [F-18]NaF PET/CT scans prior to treatment. Image segmentation resulted in 852 ROIs, 69 of which were identified by a nuclear medicine physician as DJD. For all ROIs, various PET and CT textural features were computed. ROIs were divided into training and testing sets used to train eight different machine learning classifiers. Classifiers were evaluated based on receiver operating characteristics area under the curve (AUC), sensitivity, specificity, and positive predictive value (PPV). We also assessed the added value of including CT features in addition to PET features for training classifiers. Results: The training set consisted of 37 DJD ROIs with 475 non-DJD ROIs, and the testing set consisted of 32 DJD ROIs with 308 non-DJD ROIs. Of all classifiers, generalized linear models (GLM), decision forests (DF), and support vector machines (SVM) had the best performance. AUCs of GLM (0.929), DF (0.921), and SVM (0.889) were significantly higher than the other models (p<0.001). GLM and DF, overall, had the best sensitivity, specificity, and PPV, and gave a significantly better performance (p<0.01) than all other models. PET/CT GLM classifiers had higher AUC than just PET or just CT. GLMs built using PET/CT information had superior or comparable sensitivities, specificities and PPVs to just PET or just CT. Conclusion: Machine learning algorithms trained with PET/CT features were able to identify some cases of DJD. GLM outperformed the other classification algorithms. Using PET and CT information together was shown to be superior to using PET or CT features alone. Research supported by the Prostate

  13. Milk production and distribution in low-dose counties for the Hanford Thyroid Disease Study. Hanford Environmental Dose Reconstruction Project

    SciTech Connect (OSTI)

    Schimmel, J.G.; Beck, D.M.

    1992-06-01

    This report identifies sources of milk consumed by residents of Ferry, Okanogan, and Stevens Counties. This information will be used by the Hanford thyroid Disease Study to determine whether thyroid disease has been increased among people exposed to past iodine--131 emissions from Hanford Site Facilities.

  14. Septal myocardial perfusion imaging with thallium-201 in the diagnosis of proximal left anterior descending coronary artery disease

    SciTech Connect (OSTI)

    Pichard, A.D.; Wiener, I.; Martinez, E.; Horowitz, S.; Patterson, R.; Meller, J.; Goldsmith, S.J.; Gorlin, R.; Herman, M.V.

    1981-07-01

    The use of myocardial perfusion imaging (MPI) to identify obstructive coronary disease of the left anterior descending coronary artery proximal to the first septal perforator (prox LAD) was studied in 60 patients. Perfusion of the septum and anteroapical areas with thallium-201 injected during exercise was compared to results of coronary arteriography. Septal MPI defect was found in 92.3% of patients with obstruction of the proximal LAD, 27.7% of patients with obstruction of LAD distal to first septal perforator, 0% in patients with obstructions involving right or circumflex arteries, and in 10.5% of patients without coronary disease. Anteroapical MPI defects were found with similar frequency in the three groups with obstructive coronary disease. Septal MPI defect had a sensitivity of 92.3% and specificity of 85.4% in the diagnosis of proximal LAD disease. Normal septal perfusion with thallium-201 virtually excluded proximal LAD disease.

  15. Portable microfluidic raman system for rapid, label-free early disease signature detection

    SciTech Connect (OSTI)

    Wu, Meiye; Davis, Ryan Wesley; Hatch, Anson

    2015-09-01

    In the early stages of infection, patients develop non-specific or no symptoms at all. While waiting for identification of the infectious agent, precious window of opportunity for early intervention is lost. The standard diagnostics require affinity reagents and sufficient pathogen titers to reach the limit of detection. In the event of a disease outbreak, triaging the at-risk population rapidly and reliably for quarantine and countermeasure is more important than the identification of the pathogen by name. To expand Sandia's portfolio of Biological threat management capabilities, we will utilize Raman spectrometry to analyze immune subsets in whole blood to rapidly distinguish infected from non-infected, and bacterial from viral infection, for the purpose of triage during an emergency outbreak. The goal of this one year LDRD is to determine whether Raman spectroscopy can provide label-free detection of early disease signatures, and define a miniaturized Raman detection system meeting requirements for low- resource settings.

  16. Occupational obstructive airway diseases in Germany: Frequency and causes in an international comparison

    SciTech Connect (OSTI)

    Latza, U.; Baur, X.

    2005-08-01

    Occupational inhalative exposures contribute to a significant proportion of obstructive airway diseases (OAD), namely chronic obstructive pulmonary disease (COPD) and asthma. The number of occupational OAD in the German industrial sector for the year 2003 are presented. Other analyses of surveillance data were retrieved from Medline. Most confirmed reports of OAD are cases of sensitizer induced occupational asthma (625 confirmed cases) followed by COPD in coal miners (414 cases), irritant induced occupational asthma (156 cases), and isocyanate asthma (54 cases). Main causes of occupational asthma in Germany comprise flour/flour constituents (35.9%), food/feed dust (9.0%), and isocyanates (6.5%). Flour and grain dust is a frequent cause of occupational asthma in most European countries and South Africa. Isocyanates are still a problem worldwide. Although wide differences in the estimated incidences between countries exist due to deficits in the coverage of occupational OAD, the high numbers necessitate improvement of preventive measures.

  17. Determining the risk of cardiovascular disease using ion mobility of lipoproteins

    DOE Patents [OSTI]

    Benner, W. Henry; Krauss, Ronald M.; Blanche, Patricia J.

    2010-05-11

    A medical diagnostic method and instrumentation system for analyzing noncovalently bonded agglomerated biological particles is described. The method and system comprises: a method of preparation for the biological particles; an electrospray generator; an alpha particle radiation source; a differential mobility analyzer; a particle counter; and data acquisition and analysis means. The medical device is useful for the assessment of human diseases, such as cardiac disease risk and hyperlipidemia, by rapid quantitative analysis of lipoprotein fraction densities. Initially, purification procedures are described to reduce an initial blood sample to an analytical input to the instrument. The measured sizes from the analytical sample are correlated with densities, resulting in a spectrum of lipoprotein densities. The lipoprotein density distribution can then be used to characterize cardiac and other lipid-related health risks.

  18. Mechanisms of disease: epithelial-mesenchymal transition and back again: does cellular plasticity fuel neoplastic progression?

    SciTech Connect (OSTI)

    Bissell, Mina J; Turley, Eva A.; Veiseh, Mandana; Radisky, Derek C.; Bissell, Mina J.

    2008-02-13

    Epithelial-mesenchymal transition (EMT) is a conversion that facilitates organ morphogenesis and tissue remodeling in physiological processes such as embryonic development and wound healing. A similar phenotypic conversion is also detected in fibrotic diseases and neoplasia, which is associated with disease progression. EMT in cancer epithelial cells often seems to be an incomplete and bi-directional process. In this Review, we discuss the phenomenon of EMT as it pertains to tumor development, focusing on exceptions to the commonly held rule that EMT promotes invasion and metastasis. We also highlight the role of the RAS-controlled signaling mediators, ERK1, ERK2 and PI3-kinase, as microenvironmental responsive regulators of EMT.

  19. Disease Mutations in Rab7 Result in Unregulated Nucleotide Exchange and Inappropriate Activation

    SciTech Connect (OSTI)

    B McCray; E Skordalakes; J Taylor

    2011-12-31

    Rab GTPases are molecular switches that orchestrate vesicular trafficking, maturation and fusion by cycling between an active, GTP-bound form, and an inactive, GDP-bound form. The activity cycle is coupled to GTP hydrolysis and is tightly controlled by regulatory proteins. Missense mutations of the GTPase Rab7 cause a dominantly inherited axonal degeneration known as Charcot-Marie-Tooth type 2B through an unknown mechanism. We present the 2.8 A crystal structure of GTP-bound L129F mutant Rab7 which reveals normal conformations of the effector binding regions and catalytic site, but an alteration to the nucleotide binding pocket that is predicted to alter GTP binding. Through extensive biochemical analysis, we demonstrate that disease-associated mutations in Rab7 do not lead to an intrinsic GTPase defect, but permit unregulated nucleotide exchange leading to both excessive activation and hydrolysis-independent inactivation. Consistent with augmented activity, mutant Rab7 shows significantly enhanced interaction with a subset of effector proteins. In addition, dynamic imaging demonstrates that mutant Rab7 is abnormally retained on target membranes. However, we show that the increased activation of mutant Rab7 is counterbalanced by unregulated, GTP hydrolysis-independent membrane cycling. Notably, disease mutations are able to rescue the membrane cycling of a GTPase-deficient mutant. Thus, we demonstrate that disease mutations uncouple Rab7 from the spatial and temporal control normally imposed by regulatory proteins and cause disease not by a gain of novel toxic function, but by misregulation of native Rab7 activity.

  20. "Artificial" brains, electrical grids, and disease modeling: Los Alamos

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    science discoveries unveiled September 15 Science discoveries unveiled "Artificial" brains, electrical grids, and disease modeling: Los Alamos science discoveries unveiled September 15 The event is an opportunity for business leaders and community members to learn about where science is heading, as well as for students to discover potential new career directions. September 8, 2009 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez

  1. Decreased hepatotoxic bile acid composition and altered synthesis in progressive human nonalcoholic fatty liver disease

    SciTech Connect (OSTI)

    Lake, April D.; Novak, Petr; Shipkova, Petia; Aranibar, Nelly; Robertson, Donald; Reily, Michael D.; Lu, Zhenqiang; Lehman-McKeeman, Lois D.; Cherrington, Nathan J.

    2013-04-15

    Bile acids (BAs) have many physiological roles and exhibit both toxic and protective influences within the liver. Alterations in the BA profile may be the result of disease induced liver injury. Nonalcoholic fatty liver disease (NAFLD) is a prevalent form of chronic liver disease characterized by the pathophysiological progression from simple steatosis to nonalcoholic steatohepatitis (NASH). The hypothesis of this study is that the classical (neutral) and alternative (acidic) BA synthesis pathways are altered together with hepatic BA composition during progression of human NAFLD. This study employed the use of transcriptomic and metabolomic assays to study the hepatic toxicologic BA profile in progressive human NAFLD. Individual human liver samples diagnosed as normal, steatosis, and NASH were utilized in the assays. The transcriptomic analysis of 70 BA genes revealed an enrichment of downregulated BA metabolism and transcription factor/receptor genes in livers diagnosed as NASH. Increased mRNA expression of BAAT and CYP7B1 was observed in contrast to decreased CYP8B1 expression in NASH samples. The BA metabolomic profile of NASH livers exhibited an increase in taurine together with elevated levels of conjugated BA species, taurocholic acid (TCA) and taurodeoxycholic acid (TDCA). Conversely, cholic acid (CA) and glycodeoxycholic acid (GDCA) were decreased in NASH liver. These findings reveal a potential shift toward the alternative pathway of BA synthesis during NASH, mediated by increased mRNA and protein expression of CYP7B1. Overall, the transcriptomic changes of BA synthesis pathway enzymes together with altered hepatic BA composition signify an attempt by the liver to reduce hepatotoxicity during disease progression to NASH. - Highlights: ? Altered hepatic bile acid composition is observed in progressive NAFLD. ? Bile acid synthesis enzymes are transcriptionally altered in NASH livers. ? Increased levels of taurine and conjugated bile acids are observed in

  2. Activation of farnesoid X receptor attenuates hepatic injury in a murine model of alcoholic liver disease

    SciTech Connect (OSTI)

    Wu, Weibin; Institutes of Biomedical Science, Fudan University, Shanghai 200032 ; Zhu, Bo; Peng, Xiaomin; Zhou, Meiling; Jia, Dongwei; Gu, Jianxin; Institutes of Biomedical Science, Fudan University, Shanghai 200032

    2014-01-03

    Highlights: FXR activity was impaired by chronic ethanol ingestion in a murine model of ALD. Activation of FXR attenuated alcohol-induced liver injury and steatosis. Activation of FXR attenuated cholestasis and oxidative stress in mouse liver. -- Abstract: Alcoholic liver disease (ALD) is a common cause of advanced liver disease, and considered as a major risk factor of morbidity and mortality worldwide. Hepatic cholestasis is a pathophysiological feature observed in all stages of ALD. The farnesoid X receptor (FXR) is a member of the nuclear hormone receptor superfamily, and plays an essential role in the regulation of bile acid, lipid and glucose homeostasis. However, the role of FXR in the pathogenesis and progression of ALD remains largely unknown. Mice were fed Lieber-DeCarli ethanol diet or an isocaloric control diet. We used a specific agonist of FXR WAY-362450 to study the effect of pharmacological activation of FXR in alcoholic liver disease. In this study, we demonstrated that FXR activity was impaired by chronic ethanol ingestion in a murine model of ALD. Activation of FXR by specific agonist WAY-362450 protected mice from the development of ALD. We also found that WAY-362450 treatment rescued FXR activity, suppressed ethanol-induced Cyp2e1 up-regulation and attenuated oxidative stress in liver. Our results highlight a key role of FXR in the modulation of ALD development, and propose specific FXR agonists for the treatment of ALD patients.

  3. A support vector machine classifier reduces interscanner variation in the HRCT classification of regional disease pattern in diffuse lung disease: Comparison to a Bayesian classifier

    SciTech Connect (OSTI)

    Chang, Yongjun; Lim, Jonghyuck; Kim, Namkug; Seo, Joon Beom; Lynch, David A.

    2013-05-15

    Purpose: To investigate the effect of using different computed tomography (CT) scanners on the accuracy of high-resolution CT (HRCT) images in classifying regional disease patterns in patients with diffuse lung disease, support vector machine (SVM) and Bayesian classifiers were applied to multicenter data. Methods: Two experienced radiologists marked sets of 600 rectangular 20 Multiplication-Sign 20 pixel regions of interest (ROIs) on HRCT images obtained from two scanners (GE and Siemens), including 100 ROIs for each of local patterns of lungs-normal lung and five of regional pulmonary disease patterns (ground-glass opacity, reticular opacity, honeycombing, emphysema, and consolidation). Each ROI was assessed using 22 quantitative features belonging to one of the following descriptors: histogram, gradient, run-length, gray level co-occurrence matrix, low-attenuation area cluster, and top-hat transform. For automatic classification, a Bayesian classifier and a SVM classifier were compared under three different conditions. First, classification accuracies were estimated using data from each scanner. Next, data from the GE and Siemens scanners were used for training and testing, respectively, and vice versa. Finally, all ROI data were integrated regardless of the scanner type and were then trained and tested together. All experiments were performed based on forward feature selection and fivefold cross-validation with 20 repetitions. Results: For each scanner, better classification accuracies were achieved with the SVM classifier than the Bayesian classifier (92% and 82%, respectively, for the GE scanner; and 92% and 86%, respectively, for the Siemens scanner). The classification accuracies were 82%/72% for training with GE data and testing with Siemens data, and 79%/72% for the reverse. The use of training and test data obtained from the HRCT images of different scanners lowered the classification accuracy compared to the use of HRCT images from the same scanner. For

  4. Changes in Gas Bubble Disease Signs for Migrating Juvenile Salmonids Experimentally Exposed to Supersaturated Gasses, 1996-1997 Progress Report.

    SciTech Connect (OSTI)

    Absolon, Randall F.

    1999-03-01

    This study was designed to answer the question of whether gas bubble disease (GBD) signs change as a result of the hydrostatic conditions juvenile salmonids encounter when they enter the turbine intake of hydroelectric projects during their downstream migration.

  5. Cadmium exposure and cardiovascular disease in the 2005 Korea National Health and Nutrition Examination Survey

    SciTech Connect (OSTI)

    Lee, Mi-Sun; Park, Sung Kyun; Hu, Howard; Department of Environmental Health Sciences, University of Michigan School of Public Health, Ann Arbor, MI ; Lee, Sundong; Department of Preventive Medicine, School of Oriental Medicine, Sangji University, Wonju, Kangwon

    2011-01-15

    Background: Limited epidemiologic data are available concerning the cardiovascular effects of cadmium exposure, although recent studies suggest associations with myocardial infarction and peripheral arterial disease. We examined the associations of cadmium exposure with cardiovascular disease in nationally representative general Korean adults. Methods: We used cross-sectional data on blood cadmium and self-reported diagnoses of ischemic heart disease (IHD), stroke, and hypertension in a sub-sample of 1908 adults, aged 20 years and older, who participated in the 2005 Korea National Health and Nutrition Examination Survey (KNHANES). We used survey logistic regression models accounting for the complex sampling design to estimate the odds ratios (OR), adjusting for age, education, income, alcohol, smoking, body mass index, waist circumference, family history of hypertension, blood pressure, and blood lead. Results: The geometric mean of blood cadmium was 1.53 {mu}g/L. After adjusting for potential confounders, an interquartile range (IQR) increase in blood cadmium (0.91 {mu}g/L) was found to be associated with an increased risk for IHD (OR 2.1, 95% confidence interval (CI) 1.3-3.4). An IQR increase in blood cadmium was found to be associated with an elevated risk for hypertension only among men (OR 1.4, 95% CI 1.1-1.8) but not among women. No association was observed with stroke in both genders. Conclusions: These findings suggest that cadmium in blood may be associated with an increased risk for IHD and hypertension in the general Korean adult population.

  6. Rapid Disease Progression With Delay in Treatment of Non-Small-Cell Lung Cancer

    SciTech Connect (OSTI)

    Mohammed, Nasiruddin [Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, MI (United States); Kestin, Larry Llyn, E-mail: lkestin@beaumont.ed [Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, MI (United States); Grills, Inga Siiner; Battu, Madhu; Fitch, Dwight Lamar [Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, MI (United States); Wong, Ching-yee Oliver [Department of Nuclear Medicine, William Beaumont Hospital, Royal Oak, MI (United States); Margolis, Jeffrey Harold [Department of Medical Oncology, William Beaumont Hospital, Royal Oak, MI (United States); Chmielewski, Gary William; Welsh, Robert James [Department of Thoracic Surgery, William Beaumont Hospital, Royal Oak, MI (United States)

    2011-02-01

    Purpose: To assess rate of disease progression from diagnosis to initiation of treatment for Stage I-IIIB non-small-cell lung cancer (NSCLC). Methods and Materials: Forty patients with NSCLC underwent at least two sets of computed tomography (CT) and 18-fluorodeoxyglucose positron emission tomography (PET) scans at various time intervals before treatment. Progression was defined as development of any new lymph node involvement, site of disease, or stage change. Results: Median time interval between first and second CT scans was 13.4 weeks, and between first and second PET scans was 9.0 weeks. Median initial primary maximum tumor dimension (MTD) was 3.5 cm (0.6-8.5 cm) with a median standardized uptake value (SUV) of 13.0 (1.7-38.5). The median MTD increased by a median of 1.0 cm (mean, 1.6 cm) between scans for a median relative MTD increase of 35% (mean, 59%). Nineteen patients (48%) progressed between scans. Rate of any progression was 13%, 31%, and 46% at 4, 8, and 16 weeks, respectively. Upstaging occurred in 3%, 13%, and 21% at these intervals. Distant metastasis became evident in 3%, 13%, and 13% after 4, 8, and 16 weeks, respectively. T and N stage were associated with progression, whereas histology, grade, sex, age, and maximum SUV were not. At 3 years, overall survival for Stage III patients with vs. without progression was 18% vs. 67%, p = 0.05. Conclusions: With NSCLC, treatment delay can lead to disease progression. Diagnosis, staging, and treatment initiation should be expedited. After 4-8 weeks of delay, complete restaging should be strongly considered.

  7. Discovery of Disease Co-occurrence Patterns from Electronic Healthcare Reimbursement Claims Data

    SciTech Connect (OSTI)

    Ramanathan, Arvind; Pullum, Laura L; Hobson, Tanner C; Quinn, Shannon; Chennubhotla, Chakra; Valkova, Silvia

    2014-01-01

    Effective public health surveillance is important for national secu- rity. With novel emerging infectious diseases being reported across different parts of the world, there is a need to build effective bio- surveillance systems that can track, monitor and report such events in a timely manner. Additionally, there is a need to identify sus- ceptible geographic regions/populations where these diseases may have a significant impact and design preemptive strategies to tackle them. With the digitization of health related information through electronic health records (EHR) and electronic healthcare claim re- imbursements (eHCR), there is a tremendous opportunity to ex- ploit these datasets for public health surveillance. In this paper, we present our analysis on the use of eHCR data for bio-surveillance by studying the 2009-2010 H1N1 pandemic flu season. We present a novel approach to extract spatial and temporal patterns of flu in- cidence across the United States (US) from eHCRs and find that a small, but distinct set of break-out patterns govern the flu and asthma incidence rates across the entire country. Further, we ob- serve a distinct temporal lag in the onset of flu when compared to asthma across geographic regions in the US. The patterns extracted from the data collectively indicate how these break-out patterns are coupled, even though the flu represents an infectious disease whereas asthma represents a typical chronic condition. Taken to- gether, our approach demonstrates how mining eHCRs can provide novel insights in tackling public health concerns.

  8. Tracers for monitoring the activity of sodium/glucose cotransporters in health and disease

    DOE Patents [OSTI]

    Wright, Ernest M; Barrio, Jorge R; Hirayama, Bruce A; Kepe, Vladimir

    2014-09-30

    Radiolabeled tracers for sodium/glucose cotransporters (SGLTs), their synthesis, and their use are provided. The tracers are methyl or ethyl pyranosides having an equatorial hydroxyl group at carbon-2 and a C 1 preferred conformation, radiolabeled with .sup.18F, .sup.123I, or .sup.124I, or free hexoses radiolabeled with .sup.18F, .sup.123I, or .sup.124. Also provided are in vivo and in vitro techniques for using these and other tracers as analytical and diagnostic tools to study glucose transport, in health and disease, and to evaluate therapeutic interventions.

  9. Epidemiology and Control of Infectious Diseases of Salmonids in the Columbia River Basin, 1987 Annual Report.

    SciTech Connect (OSTI)

    Fryer, John L.

    1989-01-01

    The Department of Microbiology at Oregon State University with funding from the Bonneville Power Administration has been conducting a study concerning the epidemiology and control of three fish pathogens which cause major disease problems in salmonids of the Columbia River basin. The pathogens studied include Cera to myxa Shasta, the myxosporean parasite which causes ceratomyxosis; Renibacterium salmoninarum, the bacterium which is the etiological agent of bacterial kidney disease; and the rhabdovirus which causes infectious hematopoietic necrosis (IHN). During this project, the host and geographic range of C. Shasta have been more precisely determined and the known geographic range has been significantly expanded. The effects of the parasite on fish migrating through the Columbia River and on their introduction into salt water have been examined. Similar studies have been conducted with R. salmoninarum and it has been shown that bacterial kidney disease occurs at all life stages of salmonids and is responsible for mortality in both fresh and salt water. It has also been demonstrated that different isolates of R. salmoninarum have different antigenic composition. Results of demonstration projects designed to control IHN by using UV treated water for early rearing of salmonid fry were equivocal. The scope of the project was considerably narrowed and focused during the past two years The project has concentrated on a study concerning the biology of C. Shasta and the identification of potential chemotherapeutants for control of bacterial kidney disease. The emphasis of work on C. Shasta has been its pathogenesis. This aspect of the parasite has been investigated using histopathologic and immunologic methodology. Mode of transmission, the nature of the infectious stage, and potential intermediate hosts of the parasite have also been areas of active research. Classes of chemotherapeutants with the highest potential for efficacy against R. salmoninarum have been

  10. Gas bubble disease in smallmouth bass and northern squawfish from the Snake and Columbia Rivers

    SciTech Connect (OSTI)

    Montgomery, J.C.; Becker, C.D.

    1980-11-01

    Supersaturation of the Columbia and Snake River systems was caused by entrainment of air into water spilling over hydroelectric dams. Total gas saturations of 100% or more have occurred during the spring in each river system. External signs of gas bubble diseases were noted in adult Smallmouth bass and northern squawfish collected from the lower Snake and mid-Columbia rivers during 1975-76. Emboli occurred beneath membranes of the opercula body, and fins of 72% of the smallmouth bass and 84% of the northern squawfish. Hemorrhage was also noted on the caudal, anal, and pectoral fins of several fish.

  11. Root Diseases and Exotic Ecosystems: Implications for Long-Term Site Productivity

    SciTech Connect (OSTI)

    Otrosina, W. J.; Garbelotto, M.

    1997-09-01

    Management activities and various land uses have taken place recently that have dramatically altered edaphic and environmental conditions under which forest tree species and ecosystems have evolved. Sequoia giganteum stands, fire suppression in this fire dependent ecosystem has resulted in increased mortality due to Heterobasidion annosum. On hypothesis is that fire suppression results in increased encroachment of true firs, easily infected by S-group Heterobasidion annosum, thereby transferring the disease via root contacts with S. giganteum. Existence of a hybrid with S and P ISG's of H. annosum may be evidence for anthropogenic influences on evolutionary pathways in this pathogen.

  12. Twin studies reveal specific imbalances in the mucosa-associated microbiota of patients with ileal Crohn's disease

    SciTech Connect (OSTI)

    Willing, B.; Halfvarson, J.; Dicksved, J.; Rosenquist, M.; Jarnerot, G.; Engstrand, L.; Tysk, C.; Jansson, J. K

    2008-08-15

    Large inter-individual variation in the composition of the intestinal microbiota between unrelated individuals has made it challenging to identify specific aspects of dysbiosis that lead to Crohn's disease. To reduce variations in exposure during establishment of the gut flora and influence of genotype, we studied the mucosaassociated microbiota of monozygotic twin pairs that were discordant (n=6) or concordant (n=4) for Crohn's disease. DNA was extracted from biopsies collected from 5 locations between the ileum and rectum. Bacterial 16S ribosomal RNA genes were amplified and community composition assessed by terminal-restriction fragment length polymorphism, cloning and sequencing and quantitative real-time PCR. The microbial compositions at all biopsy locations for each individual were similar, regardless of disease state, but there were differences between individuals. In particular, individuals with predominantly ileal Crohn's had a dramatically lower abundance (P<0.001) of Faecalibacterium prausnitzii and increased abundance (P<0.03) of Escherichia coli compared to healthy co-twins and those with Crohn's localized in the colon. This dysbiosis was significantly correlated to the disease phenotype rather than genotype. The reduced abundance of F. prausnitzii and increased abundance of E. coli are indicative of an ileal Crohn's disease phenotype, distinct from colonic Crohn's disease and the relative abundances of these specific bacterial populations are promising biomarker candidates for differential diagnosis of Crohn's and eventually customized treatment.

  13. Evolution of a genetic disease in an ethnic isolate:. beta. -Thalassemia in the Jews of Kurdistan

    SciTech Connect (OSTI)

    Rund, D.; Cohen, T.; Filon, D.; Rachmilewitz, E.; Oppenheim, A. ); Dowling, C.E.; Warren T.C.; Kazazian, H.H. Jr. ); Barak, I. )

    1991-01-01

    {beta}-Thalassemia is a hereditary disease caused by any of 90 different point mutations in the {beta}-globin gene. Specific populations generally carry a small number of mutations, the most common of which are those that are widely distributed regionally. The present study constitutes an extensive molecular characterization of this disease in a small, highly inbred ethnic group with a high incidence of {beta}-thalassemia-the Jews of Kurdistan. An unusual mutational diversity was observed. In 42 sibships 13 different mutations were identified, of which 3 are newly discovered. Four of the mutations are unique to Kurdish Jews and have not been discovered in any other population. A fifth was found outside Kurdish Jews only in an Iranian from Khuzistan, a region bordering Kurdistan. Two-thirds of the mutant chromosomes carry the mutations unique to Kurdish Jews. The authors traced the origin of the mutations to specific geographic regions within Kurdistan. This information, supported by haplotype analysis, suggests that thalassemia in central Kurdistan (northern Iraq) has evolved primarily from multiple mutational events. They conclude that several evolutionary mechanisms contributed to the evolution of {beta}-thalassemia in this small ethnic isolate.

  14. Proteomic Analysis of Lyme Disease: Global Protein Comparison of Three Strains of Borrelia burgdorferi

    SciTech Connect (OSTI)

    Jacobs, Jon M.; Yang, Xiaohua; Luft, Benjamin J.; Dunn, John J.; Camp, David G.; Smith, Richard D.

    2005-04-01

    The Borrelia burgdorferi spirochete is the causative agent of Lyme disease, the most common tick-borne disease in the United States. It has been studied extensively to help understand its pathogenicity of infection and how it can persist in different mammalian hosts. We report the proteomic analysis of the archetype B. burgdorferi B31 strain and two other strains (ND40, and JD-1) having different Borrelia pathotypes using strong cation exchange fractionation of proteolytic peptides followed by high-resolution, reversed phase capillary liquid chromatography coupled with ion trap tandem mass spectrometric (LC-MS/MS) analysis. Protein identification was facilitated by the availability of the complete B31 genome sequence. A total of 665 Borrelia proteins were identified representing ~38 % coverage of the theoretical B31 proteome. A significant overlap was observed between the identified proteins in direct comparisons between any two strains (>72%), but distinct differences were observed among identified hypothetical and outer membrane proteins of the three strains. Such a concurrent proteomic overview of three Borrelia strains based upon only the B31 genome sequence is shown to provide significant insights into the presence or absence of specific proteins and a broad overall comparison among strains.

  15. Evidence for increased recombination near the human insulin gene: implication for disease association studies

    SciTech Connect (OSTI)

    Chakravarti, A.; Elbein, S.C.; Permutt, M.A.

    1986-02-01

    Haplotypes for four new restriction site polymorphisms (detected by Rsa I, Taq I, HincII, and Sac I) and a previously identified DNA length polymorphism (5'FP), all at the insulin locus, have been studied in US Blacks, African Blacks, Caucasians, and Pima Indians. Black populations are polymorphic for all five markers, whereas the other groups are polymorphic for Rsa I, Taq I, and 5'FP only. The data suggest that approx. = 1 in 550 base pairs is variant in this region. The polymorphisms, even though located within 20 kilobases, display low levels of nonrandom association. Population genetic analysis suggests that recombination within this 20-kilobase segment occurs 24 times more frequently than expected if crossing-over occurred uniformly throughout the human genome. These findings suggest that population association between DNA polymorphisms and disease susceptibility genes near the insulin gene or structural mutations in the insulin gene will be weak. Thus, population studies would probably require large sample sizes to detect association. However, the low levels of nonrandom association increase the information content of the locus for linkage studies, which is the best alternative for discovering disease susceptibility genes.

  16. A Semiautomated Framework for Integrating Expert Knowledge into Disease Marker Identification

    SciTech Connect (OSTI)

    Wang, Jing; Webb-Robertson, Bobbie-Jo M.; Matzke, Melissa M.; Varnum, Susan M.; Brown, Joseph N.; Riensche, Roderick M.; Adkins, Joshua N.; Jacobs, Jon M.; Hoidal, John R.; Scholand, Mary Beth; Pounds, Joel G.; Blackburn, Michael R.; Rodland, Karin D.; McDermott, Jason E.

    2013-10-01

    Background. The availability of large complex data sets generated by high throughput technologies has enabled the recent proliferation of disease biomarker studies. However, a recurring problem in deriving biological information from large data sets is how to best incorporate expert knowledge into the biomarker selection process. Objective. To develop a generalizable framework that can incorporate expert knowledge into data-driven processes in a semiautomated way while providing a metric for optimization in a biomarker selection scheme. Methods. The framework was implemented as a pipeline consisting of five components for the identification of signatures from integrated clustering (ISIC). Expert knowledge was integrated into the biomarker identification process using the combination of two distinct approaches; a distance-based clustering approach and an expert knowledge-driven functional selection. Results. The utility of the developed framework ISIC was demonstrated on proteomics data from a study of chronic obstructive pulmonary disease (COPD). Biomarker candidates were identified in a mouse model using ISIC and validated in a study of a human cohort. Conclusions. Expert knowledge can be introduced into a biomarker discovery process in different ways to enhance the robustness of selected marker candidates. Developing strategies for extracting orthogonal and robust features from large data sets increases the chances of success in biomarker identification.

  17. Climate, Environmental, and Socioeconomic Change Weighing up the Balance in Vector-Borne Disease Transmission

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Parham, Paul; Waldock, Johanna; Christophides, George; Hemming, Deborah; Agusto, Folashade; Evans, Katherine J; Fefferman, Nina; Gaff, Holly; Gumel, Abba; LaDeau, Shannon; et al

    2015-01-01

    Arguably one of the most important effects of climate change is the potential impact on human health. While this is likely to take many forms, the implications for future transmission of vector-borne diseases (VBDs), given their ongoing contribution to global disease burden, are both extremely important and highly uncertain. In part, this is due not only to data limitations and methodological challenges when integrating climate-driven VBD models and climate change projections, but, perhaps most crucially, the multitude of epidemiological, ecological, and socioeconomic factors that drive VBD transmission, and this complexity has generated considerable debate over the last 10-15 years. Inmore » this article, and Theme Issue, we seek to elucidate current knowledge around this topic, identify key themes and uncertainties, evaluate ongoing challenges and open research questions, and, crucially, offer some solutions for the field moving forwards. Although many of these challenges are ubiquitous across multiple VBDs, more specific issues also arise in different vector-pathogen systems. This Theme Issue seeks to cover both, reflected in the breadth and depth of the topics and VBD-systems considered, itself strongly indicative of the challenging, but necessary, multidisciplinary nature of this research field.« less

  18. Climate, Environmental, and Socioeconomic Change Weighing up the Balance in Vector-Borne Disease Transmission

    SciTech Connect (OSTI)

    Parham, Paul; Waldock, Johanna; Christophides, George; Hemming, Deborah; Agusto, Folashade; Evans, Katherine J; Fefferman, Nina; Gaff, Holly; Gumel, Abba; LaDeau, Shannon; Lenhart, Suzanne; Mickens, Ronald; Naumova, Elena; Ostfeld, Richard; Ready, Paul; Thomas, Matthew; Velasco-Hernandez, Jorge; Edwin, Michael

    2015-01-01

    Arguably one of the most important effects of climate change is the potential impact on human health. While this is likely to take many forms, the implications for future transmission of vector-borne diseases (VBDs), given their ongoing contribution to global disease burden, are both extremely important and highly uncertain. In part, this is due not only to data limitations and methodological challenges when integrating climate-driven VBD models and climate change projections, but, perhaps most crucially, the multitude of epidemiological, ecological, and socioeconomic factors that drive VBD transmission, and this complexity has generated considerable debate over the last 10-15 years. In this article, and Theme Issue, we seek to elucidate current knowledge around this topic, identify key themes and uncertainties, evaluate ongoing challenges and open research questions, and, crucially, offer some solutions for the field moving forwards. Although many of these challenges are ubiquitous across multiple VBDs, more specific issues also arise in different vector-pathogen systems. This Theme Issue seeks to cover both, reflected in the breadth and depth of the topics and VBD-systems considered, itself strongly indicative of the challenging, but necessary, multidisciplinary nature of this research field.

  19. Hydrogen peroxide impairs autophagic flux in a cell model of nonalcoholic fatty liver disease

    SciTech Connect (OSTI)

    Jiang, Pengtao; University of Chinese Academy of Sciences, 19 Yuquan Road, Shijingshan District, Beijing 100049 ; Huang, Zhen; Zhao, Hong; Wei, Taotao

    2013-04-19

    Highlights: Free fatty acids exposure induces elevated autophagy. H{sub 2}O{sub 2} inhibits autophagic flux through impairing the fusion between autophagosomes and lysosomes. Inhibition of autophagy potentiates H{sub 2}O{sub 2}-induced cell death. -- Abstract: Nonalcoholic fatty liver disease (NAFLD) has become the leading cause of chronic liver disease, but the pathogenesis of NAFLD is not fully clear. The aim of this study was to determine whether autophagy plays a role in the pathogenesis of NAFLD. We found that the levels of autophagy were elevated in hepatoma cells upon exposure to free fatty acids, as confirmed by the increase in the number of autophagosomes. However, exposure of hepatoma cells to H{sub 2}O{sub 2} and TNF-?, two typical second hit factors, increased the initiation of autophagy but inhibited the autophagic flux. The inhibition of autophagy sensitized cells to pro-apoptotic stimuli. Taken together, our results suggest that autophagy acts as a protective mechanism in the pathogenesis of NAFLD and that impairment of autophagy might induce more severe lesions of the liver. These findings will be a benefit to the understanding of the pathogenesis of NAFLD and might suggest a strategy for the prevention and cure of NAFLD.

  20. A Semiautomated Framework for Integrating Expert Knowledge into Disease Marker Identification

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wang, Jing; Webb-Robertson, Bobbie-Jo M.; Matzke, Melissa M.; Varnum, Susan M.; Brown, Joseph N.; Riensche, Roderick M.; Adkins, Joshua N.; Jacobs, Jon M.; Hoidal, John R.; Scholand, Mary Beth; et al

    2013-01-01

    Background . The availability of large complex data sets generated by high throughput technologies has enabled the recent proliferation of disease biomarker studies. However, a recurring problem in deriving biological information from large data sets is how to best incorporate expert knowledge into the biomarker selection process. Objective . To develop a generalizable framework that can incorporate expert knowledge into data-driven processes in a semiautomated way while providing a metric for optimization in a biomarker selection scheme. Methods . The framework was implemented as a pipeline consisting of five components for the identification of signatures from integrated clustering (ISIC).more » Expert knowledge was integrated into the biomarker identification process using the combination of two distinct approaches; a distance-based clustering approach and an expert knowledge-driven functional selection. Results . The utility of the developed framework ISIC was demonstrated on proteomics data from a study of chronic obstructive pulmonary disease (COPD). Biomarker candidates were identified in a mouse model using ISIC and validated in a study of a human cohort. Conclusions . Expert knowledge can be introduced into a biomarker discovery process in different ways to enhance the robustness of selected marker candidates. Developing strategies for extracting orthogonal and robust features from large data sets increases the chances of success in biomarker identification.« less

  1. Proton CCQE data release page

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | Department of Energy Proteins' Amazing Origami Powers: Insight for Potential Disease Treatments Proteins' Amazing Origami Powers: Insight for Potential Disease Treatments October 4, 2011 - 12:46pm Addthis This is a visualization of drug molecules ("parade day-like balloons") in a simulated attack of the ribbon-like protein fibrils that are believed to be the cause of Alzheimer’s disease. <a

  2. Method for detection of dental caries and periodontal disease using optical imaging

    DOE Patents [OSTI]

    Nathel, H.; Kinney, J.H.; Otis, L.L.

    1996-10-29

    A method is disclosed for detecting the presence of active and inactive caries in teeth and diagnosing periodontal disease uses non-ionizing radiation with techniques for reducing interference from scattered light. A beam of non-ionizing radiation is divided into sample and reference beams. The region to be examined is illuminated by the sample beam, and reflected or transmitted radiation from the sample is recombined with the reference beam to form an interference pattern on a detector. The length of the reference beam path is adjustable, allowing the operator to select the reflected or transmitted sample photons that recombine with the reference photons. Thus radiation scattered by the dental or periodontal tissue can be prevented from obscuring the interference pattern. A series of interference patterns may be generated and interpreted to locate dental caries and periodontal tissue interfaces. 7 figs.

  3. Method for detection of dental caries and periodontal disease using optical imaging

    DOE Patents [OSTI]

    Nathel, Howard; Kinney, John H.; Otis, Linda L.

    1996-01-01

    A method for detecting the presence of active and inactive caries in teeth and diagnosing periodontal disease uses non-ionizing radiation with techniques for reducing interference from scattered light. A beam of non-ionizing radiation is divided into sample and reference beams. The region to be examined is illuminated by the sample beam, and reflected or transmitted radiation from the sample is recombined with the reference beam to form an interference pattern on a detector. The length of the reference beam path is adjustable, allowing the operator to select the reflected or transmitted sample photons that recombine with the reference photons. Thus radiation scattered by the dental or periodontal tissue can be prevented from obscuring the interference pattern. A series of interference patterns may be generated and interpreted to locate dental caries and periodontal tissue interfaces.

  4. Low Prevalence of Chronic Beryllium Disease among Workers at a Nuclear Weapons Research and Development Facility

    SciTech Connect (OSTI)

    Arjomandi, M; Seward, J P; Gotway, M B; Nishimura, S; Fulton, G P; Thundiyil, J; King, T E; Harber, P; Balmes, J R

    2010-01-11

    To study the prevalence of beryllium sensitization (BeS) and chronic beryllium disease (CBD) in a cohort of workers from a nuclear weapons research and development facility. We evaluated 50 workers with BeS with medical and occupational histories, physical examination, chest imaging with HRCT (N=49), and pulmonary function testing. Forty of these workers also underwent bronchoscopy for bronchoalveolar lavage (BAL) and transbronchial biopsies. The mean duration of employment at the facility was 18 yrs and the mean latency (from first possible exposure) to time of evaluation was 32 yrs. Five of the workers had CBD at the time of evaluation (based on histology or HRCT); three others had evidence of probable CBD. These workers with BeS, characterized by a long duration of potential Be exposure and a long latency, had a low prevalence of CBD.

  5. Method and system for the diagnosis of disease using retinal image content and an archive of diagnosed human patient data

    DOE Patents [OSTI]

    Tobin, Kenneth W; Karnowski, Thomas P; Chaum, Edward

    2013-08-06

    A method for diagnosing diseases having retinal manifestations including retinal pathologies includes the steps of providing a CBIR system including an archive of stored digital retinal photography images and diagnosed patient data corresponding to the retinal photography images, the stored images each indexed in a CBIR database using a plurality of feature vectors, the feature vectors corresponding to distinct descriptive characteristics of the stored images. A query image of the retina of a patient is obtained. Using image processing, regions or structures in the query image are identified. The regions or structures are then described using the plurality of feature vectors. At least one relevant stored image from the archive based on similarity to the regions or structures is retrieved, and an eye disease or a disease having retinal manifestations in the patient is diagnosed based on the diagnosed patient data associated with the relevant stored image(s).

  6. Disease Control After Reduced Volume Conformal and Intensity Modulated Radiation Therapy for Childhood Craniopharyngioma

    SciTech Connect (OSTI)

    Merchant, Thomas E.; Kun, Larry E.; Hua, Chia-Ho; Wu, Shengjie; Xiong, Xiaoping; Sanford, Robert A.; Boop, Frederick A.

    2013-03-15

    Purpose: To estimate the rate of disease control after conformal radiation therapy using reduced clinical target volume (CTV) margins and to determine factors that predict for tumor progression. Methods and Materials: Eighty-eight children (median age, 8.5 years; range, 3.2-17.6 years) received conformal or intensity modulated radiation therapy between 1998 and 2009. The study group included those prospectively treated from 1998 to 2003, using a 10-mm CTV, defined as the margin surrounding the solid and cystic tumor targeted to receive the prescription dose of 54 Gy. The CTV margin was subsequently reduced after 2003, yielding 2 groups of patients: those treated with a CTV margin greater than 5 mm (n=26) and those treated with a CTV margin less than or equal to 5 mm (n=62). Disease progression was estimated on the basis of additional variables including sex, race, extent of resection, tumor interventions, target volume margins, and frequency of weekly surveillance magnetic resonance (MR) imaging during radiation therapy. Median follow-up was 5 years. Results: There was no difference between progression-free survival rates based on CTV margins (>5 mm vs ≤5 mm) at 5 years (88.1% ± 6.3% vs 96.2% ± 4.4% [P=.6386]). There were no differences based on planning target volume (PTV) margins (or combined CTV plus PTV margins). The PTV was systematically reduced from 5 to 3 mm during the time period of the study. Factors predictive of superior progression-free survival included Caucasian race (P=.0175), no requirement for cerebrospinal fluid shunting (P=.0066), and number of surveillance imaging studies during treatment (P=.0216). Patients whose treatment protocol included a higher number of weekly surveillance MR imaging evaluations had a lower rate of tumor progression. Conclusions: These results suggest that targeted volume reductions for radiation therapy using smaller margins are feasible and safe but require careful monitoring. We are currently investigating

  7. The plant decapeptide OSIP108 prevents copper-induced toxicity in various models for Wilson disease

    SciTech Connect (OSTI)

    Spincemaille, Pieter; Pham, Duc-Hung; Chandhok, Gursimran; Verbeek, Jef; Zibert, Andree; Libbrecht, Louis; Schmidt, Hartmut; Esguerra, Camila V.; Witte, Peter A.M. de; Cammue, Bruno P.A.; Cassiman, David; Thevissen, Karin

    2014-10-15

    Background: Wilson disease (WD) is caused by accumulation of excess copper (Cu) due to a mutation in the gene encoding the liver Cu transporter ATP7B, and is characterized by acute liver failure or cirrhosis and neuronal cell death. We investigated the effect of OSIP108, a plant derived decapeptide that prevents Cu-induced apoptosis in yeast and human cells, on Cu-induced toxicity in various mammalian in vitro models relevant for WD and in a Cu-toxicity zebrafish larvae model applicable to WD. Methods: The effect of OSIP108 was evaluated on viability of various cell lines in the presence of excess Cu, on liver morphology of a Cu-treated zebrafish larvae strain that expresses a fluorescent reporter in hepatocytes, and on oxidative stress levels in wild type AB zebrafish larvae. Results: OSIP108 increased not only viability of Cu-treated CHO cells transgenically expressing ATP7B and the common WD-causing mutant ATP7B{sup H1069Q}, but also viability of Cu-treated human glioblastoma U87 cells. Aberrancies in liver morphology of Cu-treated zebrafish larvae were observed, which were further confirmed as Cu-induced hepatotoxicity by liver histology. Injections of OSIP108 into Cu-treated zebrafish larvae significantly increased the amount of larvae with normal liver morphology and decreased Cu-induced production of reactive oxygen species. Conclusions: OSIP108 prevents Cu-induced toxicity in in vitro models and in a Cu-toxicity zebrafish larvae model applicable to WD. General significance: All the above data indicate the potential of OSIP108 as a drug lead for further development as a novel WD treatment. - Highlights: • Wilson disease (WD) is characterized by accumulation of toxic copper (Cu). • OSIP108 increases viability of Cu-treated cellular models applicable to WD. • OSIP108 injections preserve liver morphology of Cu-treated zebrafish larvae. • OSIP108 injections into zebrafish larvae abrogates Cu-induced oxidative stress.

  8. Modeling oscillatory dynamics in brain microcircuits as a way to help uncover neurological disease mechanisms: A proposal

    SciTech Connect (OSTI)

    Skinner, F. K.; Department of Medicine , University of Toronto, 200 Elizabeth Street, Toronto, Ontario M5G 2C4; Department of Physiology, University of Toronto Medical Sciences Building, 3rd Floor, 1 King's College Circle, Toronto, Ontario M5S 1A8 ; Ferguson, K. A.; Department of Physiology, University of Toronto Medical Sciences Building, 3rd Floor, 1 King's College Circle, Toronto, Ontario M5S 1A8

    2013-12-15

    There is an undisputed need and requirement for theoretical and computational studies in Neuroscience today. Furthermore, it is clear that oscillatory dynamical output from brain networks is representative of various behavioural states, and it is becoming clear that one could consider these outputs as measures of normal and pathological brain states. Although mathematical modeling of oscillatory dynamics in the context of neurological disease exists, it is a highly challenging endeavour because of the many levels of organization in the nervous system. This challenge is coupled with the increasing knowledge of cellular specificity and network dysfunction that is associated with disease. Recently, whole hippocampus in vitro preparations from control animals have been shown to spontaneously express oscillatory activities. In addition, when using preparations derived from animal models of disease, these activities show particular alterations. These preparations present an opportunity to address challenges involved with using models to gain insight because of easier access to simultaneous cellular and network measurements, and pharmacological modulations. We propose that by developing and using models with direct links to experiment at multiple levels, which at least include cellular and microcircuit, a cycling can be set up and used to help us determine critical mechanisms underlying neurological disease. We illustrate our proposal using our previously developed inhibitory network models in the context of these whole hippocampus preparations and show the importance of having direct links at multiple levels.

  9. Cardiovascular and Interventional Radiological Society of Europe Guidelines on Endovascular Treatment in Aortoiliac Arterial Disease

    SciTech Connect (OSTI)

    Rossi, Michele; Iezzi, Roberto

    2013-11-06

    PurposeThese guidelines are intended for use in assessing the standard for technical success and safety in aorto-iliac percutaneous endovascular interventions.MethodsAny recommendation contained in the text comes from the highest level and extension of literature review available to date.ResultsThe success of endovascular procedures is strictly related to an accurate planning based mainly on CT- or MR-angiography. TASC II A through C lesions have an endovascular-first option Pre-procedure ASA antiplatelet therapy is advisable in all cases. The application of stents improves the immediate hemodynamic and most likely long-term clinical results. Cumulative mean complication rate is 7.51% according to the most relevant literature. Most of the complications can be managed by means of percutaneous techniques.ConclusionThe design and quality of devices, as well as the easy and accuracy of performing these procedures, have improved over the last decades, leading to the preferential treatment of aorto-iliac steno-obstructive disease via endovascular means, often as first-line therapy, with high technical success rate and low morbidity. This is mirrored by the decreasing number of patients undergoing surgical grafts over the last years with patency, limb salvage, and survival rates equivalent to open reconstruction.

  10. Etiology of Early Lifestage Diseases, Project 84-44, 1985 Final Report.

    SciTech Connect (OSTI)

    Sauter, R.W.

    1986-10-01

    Each year hatcheries experience loss of eggs, fry and fingerlings due to a group of poorly defined diseases called White Spot and Coagulated Yolk. Samples of maternal blood and ovarian fluid (coelomic fluid), as well as unfertilized eggs, were collected at spawning and subsequently tested for the presence of bacteria. Our tests reveal that there is a wide range in mortality rates experienced by the progeny of different brood salmon. Microbiologic tests revealed that these eggs contained a variety of both Gram positive and Gram negative bacteria within their yolk and that the fluids from the females who produced these lots were contaminated with a variety of bacteria. In contrast the eggs and maternal fluids from the six egg lots which experienced the lowest mortalities did not contain high numbers of Gram positive bacteria and contributed only 5% of the total mortalities observed within the 30 egg lots tested. From the 60 egg lots tested over two brood years we have isolated 18 different bacterial genera containing 32 different species from within the yolk surface sterilized, unfertilized eggs. Our tests suggest that Aeromonas hydrophila, Pseudomonas (3 species) Staphylococcus aureus, Vibrio sp., Corynebacterium hoffmanii, Listeria sp. and Bacillus sp. when detected within the yolk of eggs sampled from egg lots prior to fertilization will be associated with higher than normal mortality rates when the remainder of the egg lots containing these bacteria are incubated and reared. 36 refs., 16 figs.

  11. Thermodynamics imprinting reveals differential binding of metals to {alpha}-synuclein: Relevance to parkinson's disease

    SciTech Connect (OSTI)

    Bharathi; Rao, K.S.J. . E-mail: kjr5n@yahoo.co.in

    2007-07-20

    The aggregation of {alpha}-synuclein is a hallmark feature of Parkinson's disease (PD) and other synucleinopathies. Metals are the significant etiological factors in PD, and their interaction with {alpha}-synuclein affect dramatically the kinetics of fibrillation in vitro and are proposed to play an important and potential neurodegenerative role in vivo. In the present study, we investigated the stoichiometry of binding of copper [Cu (II)] and iron [Fe (III)] with {alpha}-synuclein (wild recombinant type and A30P, A53T, E46K mutant forms) using isothermal titration calorimetry (ITC). {alpha}-Synuclein monomer (wild and mutant forms) titrated by Cu (II), showed two binding sites, with an apparent K {sub B} of 10{sup 5} M and 10{sup 4} M, respectively. But, {alpha}-synuclein (wild type and mutant forms) titrated with Fe (III) revealed a K {sub B} of 10{sup 5} M with single binding site. The present investigation uncovers the detailed binding propensities between metals and {alpha}-synuclein and has biological implications in PD.

  12. Analysis of HLA-DP association with beryllium disease susceptibility in pooled exposed populations

    SciTech Connect (OSTI)

    Cesare Saltini, Massimo Amicosante

    2009-12-19

    Berylliosis or Chronic Beryllium Disease is a chronic granulomatous disorder primarily involving the lung associated with the exposition to low doses of Beryllium (Be) in the workplace. Berylliosis risk has been associated with the presence of a glutamate at position 69 of the HLA-DP beta chain (HLA-DPbetaGlu69) that is expressed in about 97% of disease cases and in 27% of the unaffected Be-exposed controls (p<0.0001) (Richeldi et al. Science 1993; 262: 242-244.12). Since this first observation of an immunogenetic association between berylliosis and HLA-DPbetaGlu69 a number of studies have confirmed the role of this marker as the primary gene of susceptibility of berylliosis (Richeldi et al Am J Ind Med. 1997; 32:337-40; Wang et al J. Immunol. 1999; 163: 1647-53; Saltini et al Eur Respir J. 2001 18:677-84; Rossman et al Am J Respir Crit Care Med. 2002 165:788-94). Moreover, a structure/function interaction between HLA-DP molecules carrying Glu69 and beryllium in driving and developing the immune response against beryllium itself has been observed as: (1) Be-specific T-cells clones obtained from berylliosis patients recognize beryllium as antigen only when presented in the context of the HLA-DP{beta}Glu69 molecules but not in the context of HLA-DP allelic variants carrying Lys69 (Lombardi G et al. J Immunol 2001; 166: 3549-3555), and (2) beryllium presents an affinity for the HLA-DP2, carrying the berylliosis marker of susceptibility HLA-DPGlu69, from 40 to 100 times higher that the HLA-DP molecule carrying Lys69 (Amicosante M. et al Hum. Immunol. 2001; 62: 686-93). However, although the immunogenetic studies performed have been addressed a number of different questions about the genetic association between berylliosis and/or beryllium sensitization, exposure levels to beryllium and HLA markers, a number of questions are still open in the field mainly due to the limitation imposed by the low number of subjects carrying berylliosis or beryllium sensitization enrolled

  13. Structure of the Newcastle disease virus F protein in the post-fusion conformation

    SciTech Connect (OSTI)

    Swanson, Kurt; Wen, Xiaolin; Leser, George P.; Paterson, Reay G.; Lamb, Robert A.; Jardetzky, Theodore S. (Stanford-MED); (NWU); (HHMI)

    2010-11-17

    The paramyxovirus F protein is a class I viral membrane fusion protein which undergoes a significant refolding transition during virus entry. Previous studies of the Newcastle disease virus, human parainfluenza virus 3 and parainfluenza virus 5 F proteins revealed differences in the pre- and post-fusion structures. The NDV Queensland (Q) F structure lacked structural elements observed in the other two structures, which are key to the refolding and fusogenic activity of F. Here we present the NDV Australia-Victoria (AV) F protein post-fusion structure and provide EM evidence for its folding to a pre-fusion form. The NDV AV F structure contains heptad repeat elements missing in the previous NDV Q F structure, forming a post-fusion six-helix bundle (6HB) similar to the post-fusion hPIV3 F structure. Electrostatic and temperature factor analysis of the F structures points to regions of these proteins that may be functionally important in their membrane fusion activity.

  14. Human borna disease virus infection impacts host proteome and histone lysine acetylation in human oligodendroglia cells

    SciTech Connect (OSTI)

    Liu, Xia; Zhao, Libo; Yang, Yongtao; Bode, Liv; Huang, Hua; Liu, Chengyu; Huang, Rongzhong; Zhang, Liang; and others

    2014-09-15

    Background: Borna disease virus (BDV) replicates in the nucleus and establishes persistent infections in mammalian hosts. A human BDV strain was used to address the first time, how BDV infection impacts the proteome and histone lysine acetylation (Kac) of human oligodendroglial (OL) cells, thus allowing a better understanding of infection-driven pathophysiology in vitro. Methods: Proteome and histone lysine acetylation were profiled through stable isotope labeling for cell culture (SILAC)-based quantitative proteomics. The quantifiable proteome was annotated using bioinformatics. Histone acetylation changes were validated by biochemistry assays. Results: Post BDV infection, 4383 quantifiable differential proteins were identified and functionally annotated to metabolism pathways, immune response, DNA replication, DNA repair, and transcriptional regulation. Sixteen of the thirty identified Kac sites in core histones presented altered acetylation levels post infection. Conclusions: BDV infection using a human strain impacted the whole proteome and histone lysine acetylation in OL cells. - Highlights: • A human strain of BDV (BDV Hu-H1) was used to infect human oligodendroglial cells (OL cells). • This study is the first to reveal the host proteomic and histone Kac profiles in BDV-infected OL cells. • BDV infection affected the expression of many transcription factors and several HATs and HDACs.

  15. Similarities of host defense mechanisms against pulmonary infectious disease in animals and man

    SciTech Connect (OSTI)

    Green, G.M.

    1984-01-01

    Evidence linking exposure to air pollutants with increased susceptibility to infectious diseases in humans comes from epidemiological, clinical, and experimental laboratory studies. The data suggest that the most common, and perhaps the most sensitive, index of the pulmonary effect of air pollutant exposure is on post upper respiratory infection, prolonged cough, phlegm, and purulent sputum. Experimental models of these relationships for extrapolation to humans should be able to measure such minor changes in symptomatology and physiology rather than require major lethal events. The bacterial aerosol model for quantifying nonspecific defense mechanisms of the bronchopulmonary tree utilizing nonpathogenic organisms fulfills this criterion. The function of the six major components of pulmonary antimicrobial defense mechanisms - including aerodynamic filtration, secretory respiratory tract fluid, fluid transport at the alveolar and bronchial levels, the phagocytic function of alveolar macrophages, the augmenting mechanisms of blood-derived inflammatory cells, and the secretory and cellular-specific immune mechanisms and their mediator products - can all be quantified by this experimental animal model system. The defensive functions are remarkably similar across animal species, and available human data suggest that findings obtained using the model may be extrapolatred to humans.

  16. Memorandum, Clarification of Title 10 of the Code of Federal Regulations, Part 850 (10 CPR 850), Chronic Beryllium Disease Prevention Program, Paragraph 850.34(g)

    Broader source: Energy.gov [DOE]

    The purpose of this memorandum is to clarification regarding the reporting of beryllium sensitization (BeS) and chronic beryllium disease (CBD) as required by paragraph 850.34(g) of the Chronic Beryllium Disease Prevention Program rule (10 CFR 850).

  17. The Impact of Preradiation Residual Disease Volume on Time to Locoregional Failure in Cutaneous Merkel Cell CarcinomaA TROG Substudy

    SciTech Connect (OSTI)

    Finnigan, Renee; Hruby, George; Wratten, Chris; Keller, Jacqui; Tripcony, Lee; Dickie, Graeme; Rischin, Danny; Poulsen, Michael

    2013-05-01

    Purpose: This study evaluated the impact of margin status and gross residual disease in patients treated with chemoradiation therapy for high-risk stage I and II Merkel cell cancer (MCC). Methods and Materials: Data were pooled from 3 prospective trials in which patients were treated with 50 Gy in 25 fractions to the primary lesion and draining lymph nodes and 2 schedules of carboplatin based chemotherapy. Time to locoregional failure was analyzed according to the burden of disease at the time of radiation therapy, comparing patients with negative margins, involved margins, or macroscopic disease. Results: Analysis was performed on 88 patients, of whom 9 had microscopically positive resection margins and 26 had macroscopic residual disease. The majority of gross disease was confined to nodal regions. The 5-year time to locoregional failure, time to distant failure, time to progression, and disease-specific survival rates for the whole group were 73%, 69%, 62%, and 66% respectively. The hazard ratio for macroscopic disease at the primary site or the nodes was 1.25 (95% confidence interval 0.57-2.77), P=.58. Conclusions: No statistically significant differences in time to locoregional failure were identified between patients with negative margins and those with microscopic or gross residual disease. These results must, however, be interpreted with caution because of the limited sample size.

  18. Genome Stability of Lyme Disease Spirochetes: Comparative Genomics of Borrelia burgdorferi Plasmids

    SciTech Connect (OSTI)

    Casjens S. R.; Dunn J.; Mongodin, E. F.; Qiu, W.-G.; Luft, B. J.; Schutzer, S. E.; Gilcrease, E. B.; Huang, W. M.; Vujadinovic, M.; Aron, J. K.; Vargas, L. C.; Freeman, S.; Radune, D.; Weidman, J. F.; Dimitrov, G. I.; Khouri, H. M.; Sosa, J. E.; Halpin, R. A.; Fraser, C. M.

    2012-03-14

    Lyme disease is the most common tick-borne human illness in North America. In order to understand the molecular pathogenesis, natural diversity, population structure and epizootic spread of the North American Lyme agent, Borrelia burgdorferi sensu stricto, a much better understanding of the natural diversity of its genome will be required. Towards this end we present a comparative analysis of the nucleotide sequences of the numerous plasmids of B. burgdorferi isolates B31, N40, JD1 and 297. These strains were chosen because they include the three most commonly studied laboratory strains, and because they represent different major genetic lineages and so are informative regarding the genetic diversity and evolution of this organism. A unique feature of Borrelia genomes is that they carry a large number of linear and circular plasmids, and this work shows that strains N40, JD1, 297 and B31 carry related but non-identical sets of 16, 20, 19 and 21 plasmids, respectively, that comprise 33-40% of their genomes. We deduce that there are at least 28 plasmid compatibility types among the four strains. The B. burgdorferi {approx}900 Kbp linear chromosomes are evolutionarily exceptionally stable, except for a short {le}20 Kbp plasmid-like section at the right end. A few of the plasmids, including the linear lp54 and circular cp26, are also very stable. We show here that the other plasmids, especially the linear ones, are considerably more variable. Nearly all of the linear plasmids have undergone one or more substantial inter-plasmid rearrangements since their last common ancestor. In spite of these rearrangements and differences in plasmid contents, the overall gene complement of the different isolates has remained relatively constant.

  19. Raaometry C. D. Whiteman, J. M. Alzheimer, G. A. Anderson, and...

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    leveling is encountered during clear daytime conditions. In these conditions, the mean tilt of the radiometer must be less than 1 0 with oscillations less than about 20 in...

  20. Data:1fff44fd-9121-43ad-ad10-1fe40e158c93 | Open Energy Information

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