GEOTHERMAL TECHNOLOGIES LEGACY COLLECTION - Bibliographic Citation


Bibliographic Citation


Full Text: This document is not available electronically. More about document availability.
Title: Identification of Novel Positive-Strand RNA Viruses by Metagenomic Analysis of Archaea-Dominated Yellowstone Hot Springs
Creator/Author: Benjamin Bolduc ; Daniel P. Shaughnessy ; Yuri I. Wolf ; Eugene V. Koonin ; Francisco F. Roberto ; Mark Young
Publication Date:2012 May 01
OSTI Identifier:OSTI 1044918
Report Number(s):INL/JOU-12-26485
DOE Contract Number:DE-AC07-05ID14517
Document Type:Journal Article
Specific Type:
Coverage:
Resource Relation:Journal Name: Journal of Virology; Journal Volume: 86; Journal Issue: 10
Other Number(s):
Research Org:Idaho National Laboratory (INL)
Sponsoring Org:DOE - NE
Subject:15 GEOTHERMAL ENERGY; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; BACTERIA; COMMUNITIES; CONTIGS; GENES; HOT SPRINGS; NUCLEOTIDES; ORIGIN; PROTEINS; RNA; RNA POLYMERASES; VIRUSES; YELLOWSTONE NATIONAL PARK
Keywords:RNA Viruses
Description/Abstract:There are no known RNA viruses that infect Archaea. Filling this gap in our knowledge of viruses will enhance our understanding of the relationships between RNA viruses from the three domains of cellular life and, in particular, could shed light on the origin of the enormous diversity of RNA viruses infecting eukaryotes. We describe here the identification of novel RNA viral genome segments from high-temperature acidic hot springs in Yellowstone National Park in the United States. These hot springs harbor low-complexity cellular communities dominated by several species of hyperthermophilic Archaea. A viral metagenomics approach was taken to assemble segments of these RNA virus genomes from viral populations isolated directly from hot spring samples. Analysis of these RNA metagenomes demonstrated unique gene content that is not generally related to known RNA viruses of Bacteria and Eukarya. However, genes for RNA-dependent RNA polymerase (RdRp), a hallmark of positive-strand RNA viruses, were identified in two contigs. One of these contigs is approximately 5,600 nucleotides in length and encodes a polyprotein that also contains a region homologous to the capsid protein of nodaviruses, tetraviruses, and birnaviruses. Phylogenetic analyses of the RdRps encoded in these contigs indicate that the putative archaeal viruses form a unique group that is distinct from the RdRps of RNA viruses of Eukarya and Bacteria. Collectively, our findings suggest the existence of novel positive-strand RNA viruses that probably replicate in hyperthermophilic archaeal hosts and are highly divergent from RNA viruses that infect eukaryotes and even more distant from known bacterial RNA viruses. These positive-strand RNA viruses might be direct ancestors of RNA viruses of eukaryotes.
Publisher:
Country of Publication:US
Language:English
Size/Format:Medium: X; Size: 5562 - 5573
Rights:
Availability:
System Entry Date:2012 Dec 05
 View Non-Empty Fields Only

Top

Some links on this page may take you to non-federal websites. Their policies may differ from this site.