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Citation URL: http://www.osti.gov/geothermal/product.biblio.jsp?osti_id=1050725
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| Title: |
Phase Preference by Active, Acetate-Utilizing Bacteria at the Rifle, CO Integrated
Field Research Challenge Site |
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| Creator/Author: |
Kerkhof, L.
;
Williams, K.H.
;
Long, P.E.
;
McGuinness, L.
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| Publication Date: | 2011 Feb 21 |
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| OSTI Identifier: | OSTI 1050725 |
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| Report Number(s): | LBNL-4853E |
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| DOE Contract Number: | DE-AC02-05CH11231 |
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| Document Type: | Journal Article |
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| Specific Type: | |
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| Coverage: | |
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| Resource Relation: | Journal Name: Environmental Science and Technology; Journal Volume: 45; Journal Issue: 4; Related Information: Journal Publication Date: 2011 |
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| Other Number(s): | |
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| Research Org: | Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US) |
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| Sponsoring Org: | Earth Sciences Division |
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| Subject: | 15 GEOTHERMAL ENERGY; 54 ENVIRONMENTAL SCIENCES; ACETATES; BACTERIA; DNA; SAND; SEDIMENTS; STABLE ISOTOPES; URANIUM |
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| Keywords: | |
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| Description/Abstract: | Previous experiments at the Rifle, Colorado Integrated Field Research Challenge (IFRC) site demonstrated that field-scale addition of acetate to groundwater reduced the ambient soluble uranium concentration. In this report, sediment samples collected before and after acetate field addition were used to assess the active microbes via {sup 13}C acetate stable isotope probing on 3 phases [coarse sand, fines (8-approximately 150 {micro}m), groundwater (0.2-8 {micro}m)] over a 24-day time frame. TRFLP results generally indicated a stronger signal in {sup 13}C-DNA in the 'fines' fraction compared to the sand and groundwater. Before the field-scale acetate addition, a Geobacter-like group primarily synthesized {sup 13}C-DNA in the groundwater phase, an alpha Proteobacterium primarily grew on the fines/sands, and an Acinetobacter sp. and Decholoromonas-like OTU utilized much of the {sup 13}C acetate in both groundwater and particle-associated phases. At the termination of the field-scale acetate addition, the Geobacter-like species was active on the solid phases rather than the groundwater, while the other bacterial groups had very reduced newly synthesized DNA signal. These findings will help to delineate the acetate utilization patterns of bacteria in the field and can lead to improved methods for stimulating distinct microbial populations in situ. |
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| Publisher: | |
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| Country of Publication: | US |
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| Language: | English |
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| Size/Format: | Medium: ED; Size: 1250 |
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| Rights: | |
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| Availability: | |
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| System Entry Date: | 2012 Dec 05 |
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