Lamond, Angus I. - Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee

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Lamond, Angus I. - Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee

UNIT 12.10Detecting Protein-Protein Interactions In Vivo with FRET using Multiphoton
retarded, phenotype in which many normal cell fate decisions are
Chromatin Isolation by small-scale biochemical Fractionation (Source: Wysocka J et al.: Loss of HCF-1-Chromatin Association Precedes Temperature-
INTRODUCTION Reversible protein phosphorylation catalysed by protein
Identication of peptide inhibitors of pre-mRNA splicing derived from the essential interaction
A Proteomic Study of SUMO-2 Target Proteins*S Received for publication, April 15, 2004, and in revised form, June 1, 2004
Cellular Fractionation Lamond Lab Protocol 2007
TheJournalofCellBiology The Rockefeller University Press, 0021-9525/2004/09/787/14 $8.00
Fluorophore Conjugation of Antibodies (Lamond Lab) (here Alexa conjugation of IgG antibodies)
The Rockefeller University Press, 0021-9525/2002/05/615/15 $5.00 The Journal of Cell Biology, Volume 157, Number 4, May 13, 2002 615629
Quick guide The nucleolus
Newly assembled snRNPs associate with coiled bodies before speckles, suggesting a nuclear snRNP maturation pathway
Nucleolar Proteome Database (http://www.lamondlab.com/NoPDB) August 2004 Acc # Acc # IPI Protein
4. Isotopically-Labeled Amino Acids (for R6K6, R6K4 and R10K8 media) Source: Cambridge Isotope Lab (CIL; North America; www.isotope.com). UK distributor of CIL is: CK GAS Products Ltd. (3 Murrell Green Business Park,
SPF30 Is an Essential Human Splicing Factor Required for Assembly of the U4/U5/U6 Tri-small Nuclear Ribonucleoprotein into
3D3/lyric: a novel transmembrane protein of the endoplasmic reticulum and nuclear envelope, which is also present in the nucleolus
Cell, Vol. 109, 285296, May 3, 2002, Copyright 2002 by Cell Press Splicing Regulation at the Second Catalytic Step
Characterization of hCINAP, a Novel Coilin-interacting Protein Encoded by a Transcript from the Transcription
10.1101/gr.473902Access the most recent version at doi: 2002 12: 1231-1245; originally published online Jul 19, 2002;Genome Res.
In-gel digestion of proteins separated by (Lamond Lab / May 2007)
THEJOURNALOFCELLBIOLOGY JCB: ARTICLE
Covalently Conjugate Antibody to Beads A Lamond Lab protocol (www.lamondlab.com; 2007)
Distinct and Overlapping Sets of SUMO-1 and SUMO-2 Target Proteins Revealed by Quantitative Proteomics
Pre-mRNA Splicing Protocols (Lamond Lab) www.lamondlab.com
FRET measurement between YFP and CFP EYFP and ECFP function as a donor-acceptor pair for fluorescence resonance
Nucleolar Proteome Database (http://www.lamondlab.com/NoPDB) August 2004 Acc # Acc # IPI Protein
The Rockefeller University Press, 0021-9525/2000/12/1561/14 $5.00 The Journal of Cell Biology, Volume 151, Number 7, December 25, 2000 15611574
Mitotic phosphatases: no longer silent partners Laura Trinkle-Mulcahy and Angus I Lamond
THEJOURNALOFCELLBIOLOGY JCB: ARTICLE
L E T T E R S NATURE CELL BIOLOGY VOLUME 8 | NUMBER 10 | OCTOBER 2006 1133
Molecular Biology of the Cell Vol. 16, 53045315, November 2005
10.1261/rna.7277705Access the most recent version at doi: 2005 11: 1201-1214RNA
The nucleolus Yun Wah Lam*, Laura Trinkle-
THEJOURNALOFCELLBIOLOGY JCB: ARTICLE
Biochem. J. (2005) 388, 593603 (Printed in Great Britain) 593 Interaction of U-box E3 ligase SNEV with PSMB4, the 7 subunit
Biochem. J. (2003) 376, 553569 (Printed in Great Britain) 553 REVIEW ARTICLE
Introduction The survival of motor neurons protein (SMN) is part of a
Introduction Spinal muscular atrophy (SMA) is an autosomal recessive
Molecular Biology of the Cell Vol. 14, 107117, January 2003
2Molecular Biology of the Cell Vol. 13, 24612473, July 2002
NATURE CELL BIOLOGY VOL 4 JULY 2002 http://cellbio.nature.com 502 Cajal Body dynamics and association
Yeast Pescadillo is required for multiple activities during 60S ribosomal subunit synthesis
A Direct Interaction between the Carboxyl-terminal Region of CDC5L and the WD40 Domain of PLRG1 Is Essential for
INTRODUCTION Hundreds of protein kinases and protein phosphatases are
http://genomebiology.com/2001/2/3/reviews/0002.1 Nuclear dynamics: where genes are and how they got there
PRCC, the commonest TFE3 fusion partner in papillary renal carcinoma is associated with pre-mRNA splicing factors
In-gel digestion of proteins separated by (Lamond Lab, January 2009)
PFA Fixation for Immunofluorescence * This fixation protocol works well for most antibodies and fusion proteins. We use it at
Labelling transcription sites in mammalian cells (Adapted from Koberna et al (2000) Acta Histochem 102: 15-20)
SPLICING ASSAYS (20 ul volume) 2.0 ul 20 mM MgCl2
Phosphatase Assays (using 32P-Phosphorylase a as substrate) 50 mM Tris, pH 7.5
Immunostaining: Fix cells as desired (downloadable paraformaldehyde and methanol fixation
Nucleolar Isolation Protocol We recommend that you first download and read this page as a . Using that as your
Cajal Body Isolation Protocol A step-by-step protocol and solution recipes are provided below. We have also included higher
ANRV255-CB21-05 ARI 8 September 2005 16:14 Cajal Bodies: A Long
hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing
Nuclear organization of pre-mRNA splicing factors Judith E Sleeman and Angus I Lamond*
The Rockefeller University Press, 0021-9525/2002/10/17/5 $5.00 The Journal of Cell Biology, Volume 159, Number 1, October 14, 2002 1721
Molecular Biology of the Cell Vol. 16, 260269, January 2005
INTRODUCTION Cajal bodies (also called coiled bodies) are nuclear structures
GLT1 activity and immunoblotting Levels of GLT1 protein were quantified by immunoblots3
BrUTP Labeling 100 mM KOAc (0.15 g)
Paul Ajuh, Janet Chusainow, Ursula Ryder and Angus I.Lamond1
Visualization of Intracellular PP1 Targeting 133 From: Methods in Molecular Biology, Volume 365: Protein Phosphatase Protocols
Zupfkuchen (some kind of cheese cake) What you need...
Jumbo Jaffa Cake 4 navel (seedless) oranges, small-medium size
PURIFICATION OF GST-TAGGED PROTEINS Materials/Solutions
Preparation of Nuclear Extract from Plated Cells Solution NE1
Silver staining protocol Compatible with mass spectrometry, excellent signal-to-noise ratio
LYSING CELLS FOR NUCLEAR PROTEINS Wash 10 cm dish of cells with PBS.
Susan's Lemon Poppy Seed Cake 2 oz. Black poppy seeds (4 tbs.)*
PURIFICATION OF HIS-TAGGED PROTEINS Materials/Solutions
Calcium Phosphate Transfection Works very well for 293 cells (up to 95% transfection efficiencies). Grow cells in