- PLASMID MINI PREP Inoculate 5 ml YT + Amp with single bacterial colony. Incubate at 37 C over night with
- The Snf1 kinase and proteasome-associated Rad23 regulate UV-responsive gene expression
- Snf2/Swi2-related ATPase Mot1 drives displacement of TATA-binding protein
- Total Yeast RNA Prep Grow ~ 100 ml cells to OD600 ~1.0. Heat shock or treat if desired. Streak cells
- PCR primers for KO, tagging, detection amplification of pRS400a (KanMX) or pRS403 (HIS3) for KO
- MOLECULAR AND CELLULAR BIOLOGY, Apr. 2007, p. 28862896 Vol. 27, No. 8 0270-7306/07/$08.00 0 doi:10.1128/MCB.00054-07
- Whole Cell Extract Prep of Gal-Induced Yeast Cells Inoculate 3 X 5 ml YEP + 2% raffinose with single yeast colony harboring EE-MOT1-
- MOLECULAR AND CELLULAR BIOLOGY, 0270-7306/99/$04.00 0
- Mot1-mediated control of transcription complex assembly and activity
- Coupling Py antibody to agarose beads Use Pharmacia GammaBind Plus Sepharose (cat # 17-0886-02). Binding
- MOLECULAR AND CELLULAR BIOLOGY, 0270-7306/99/$04.00 0
- Sprouse et al. Supplemental Data Supplementary Figure 1: Comparison of mot1-14 microarray datasets from Dasgupta et al.
- Purification of EE-Mot1 Using Antibody-Coupled Beads Perform all steps except quick spins and peptide elution at 4 degrees. Use about 100 mg of
- SUPPLEMENTARY FIGURES Wade et al, "The Snf1 kinase and the proteasome-associated Rad23 regulate UV-
- Genome-wide Analysis of ARS (Autonomously Replicating Sequence) Binding Factor 1 (Abf1p)-mediated Transcriptional Regulation in
- 10.1101/gr.109504.110Access the most recent version at doi: 2010 20: 1679-1688 originally published online September 20, 2010Genome Res.
- JOURNAL OF VIROLOGY, Feb. 2009, p. 18811891 Vol. 83, No. 4 0022-538X/09/$08.00 0 doi:10.1128/JVI.01805-08
- Research Focus The dynamic personality of TATA-binding protein
- Supporting Information Sprouse et al. 10.1073/pnas.0801901105
- Function and Structural Organization of Mot1 Bound to a Natural Target Promoter*S
- MOLECULAR AND CELLULAR BIOLOGY, July 2004, p. 63626378 Vol. 24, No. 14 0270-7306/04/$08.00 0 DOI: 10.1128/MCB.24.14.63626378.2004
- Sir Antagonist 1 (San1) Is a Ubiquitin Ligase* Received for publication, January 27, 2004, and in revised form, April 9, 2004
- Mot1 activates and represses transcription by direct, ATPase-dependent mechanisms
- Mot1 ATPase assay Each reaction contains
- Chromatin Immunoprecipitation Make Whole Cell Extract
- Gel Shift Assay for Detection of TBP-DNA Complexes and Mot1 activity 6% Polyacrylamide Gel (14 cm X 16 cm X 0.2 cm)
- Northern Blots (based on protocol from Mitch Smith Lab)
- Yeast Genomic DNA Prep Sterile distilled water
- TEN-MINUTE GENOMIC DNA PREPARATION -Sterile water
- Yeast Transformation Inoculate 100 ml YPD with 1 single (reasonably fat) yeast colony at about 5
- Regulation of TATA-binding protein dynamics in living yeast cells
- TATA-binding Protein Variants That Bypass the Requirement for Mot1 in Vivo*S
- In Vitro NER Assay Yeast extraction Buffer (200 ml): 0.2 M Tris-acetate (pH 7.5) (40 ml), 0.39 M
- Mot1 Regulates the DNA Binding Activity of Free TATA-binding Protein in an ATP-dependent Manner*
- 10.1101/gad.2050311Access the most recent version at doi: 2011 25: 1306-1319Genes Dev.
- This document briefly describes the data file types available from the Auble Lab web site pertaining to
- Poorey et al Supplemental Methods and Figures
