- COPAmulti v1 (for plate mean control)
- Genome-wide RNAi screening identifies protein damage as a regulator of osmoprotective
- doi:10.1152/physiolgenomics.00106.2009 40:8-14, 2009. First published Sep 15, 2009;Physiol Genomics
- Worm Genomic DNA prep Materials needed
- doi:10.1152/ajpcell.00514.2006 292:1867-1873, 2007. First published Jan 17, 2007;Am J Physiol Cell Physiol
- The Journal of Molecular Biology Research, Protocols, Reviews, and Applications Biotechnology
- doi:10.1152/ajpcell.00451.2004 288:467-474, 2005. First published Oct 20, 2004;Am J Physiol Cell Physiol
- doi:10.1152/ajpcell.00381.2003 286:785-791, 2004. First published Nov 26, 2003;Am J Physiol Cell Physiol
- ENU F2 Mutagenesis Day 1 Prepare ENU stock solutions in fresh 100% ethanol (40 mM = 6.5 mg/ml (it's
- Live/Dead Staining of C. elegans with Sytox Green and Sytox 1. Prepare a 10M solution of Sytox Green in 1X M9
- Microscopy on anesthetized worms Reagents needed
- Bacterial Transformation of DH5a Materials Needed
- Materials needed Primer A Gene specific sense (21-24bp); ~2-3Kb from start ATG
- Cracking Procedure for Rapid analysis of plasmid Materials Needed
- You will need: MATLAB (at minimum, version 7.0.1.24704, service pack 1)
- COPAcompare v1 (for comparing to plate mean)
- Instructions for the use of COPAcompare
- Instructions for the use of Elizabeth Morton and Todd Lamitina
- University of Pennsylvania DNA SEQUENCING FACILITY Tel: (215) 573-7407 Mailing Address
- One-Tube BP and LR Gateway reaction Reagents needed
- Instructions for the use of COPAcompare
- Gateway clone primer design The proper design of attB-attached primer is a crucial point for the BP and LR reaction of
- PCR confirmation of C. elegans single-copy insertions Reagents needed
- Lifespan Assay Reagents needed
- Gateway BP reaction Reagents needed
- Protocol for C. elegans cell culture and RNA isolation Reagents needed
- Instructions for the use of Elizabeth Morton and Todd Lamitina
- TheJournalofGeneralPhysiology J. Gen. Physiol. The Rockefeller University Press $8.00
- qRT-PCR setup Reagents Needed
- Growing RNAi bacteria Reagents Needed
- Instructions for the use of Elizabeth Morton and Todd Lamitina
- BCA protein assay for glycerol assays Reagents needed
- UV integration of extrachromosomal arrays Reagents needed
- Total RNA Isolation from C. elegans for Affymetrix Microarrays Using TRIzol
- Whole worm glycerol assay Reagents needed
- The Caenorhabditis elegans Mucin-Like Protein OSM-8 Negatively Regulates Osmosensitive Physiology Via the
- Detailed Instructions for the use of BMP Predrag Krajacic1
- Instructions for the use of BMP Predrag Krajacic1, Xiaoning Shen2, Prashant Purohit2,
