- Primer Extension Promega Primer Extension system AMV Reverse Transcriptase Cat.# E 3030. Includes
- Parasitology (1975), 71, 311-326 311 With 6 figures in the text
- Transposon Mutagenesis of Trypanosoma brucei Identifies Glycosylation Mutants Resistant to Concanavalin A*
- Telomere structure and shortening in telomerase-deficient Trypanosoma brucei
- Molecular and Biochemical Parasitology 91 (1998) 7791 Regulation of 6sg expression site transcription and switching in
- protocols buffers et#31F4C7.doc 1/26/06 1 General-purpose solutions
- Molecular & Biochemical Parasitology 156 (2007) 4150 Histone modifications in Trypanosoma brucei
- Instructions for Corning Model 220 pH Meter The electrode tip is a fragile glass bulb. Be careful or you will break it with a
- Introduction The basic chromatin unit the nucleosome is composed of
- Immunofluorescence There are two ways of fixing parasites for IF or FISH. Either the cells are settled onto
- Molecular & Biochemical Parasitology 114 (2001) 169181 Telomere shortening and cell cycle arrest in Trypanosoma brucei
- COMPETENT CELLS (based on Hanahan 1983 J Mol Biol 166, 557580) STREAK a fresh plate the day before inoculation from parent stock (NOT previous
- There are two ways of fixing parasites for IF or FISH. Either the cells are settled onto non-silanized coverslips (Fisher 12mm, #15) and then fixed (faster but harsher), or the
- MOLECULAR AND CELLULAR BIOLOGY, Aug. 2005, p. 73147322 Vol. 25, No. 16 0270-7306/05/$08.00 0 doi:10.1128/MCB.25.16.73147322.2005
- Molecular and Biochemical Parasitology 111 (2000) 261273 The molecular karyotype of the megabase chromosomes of
- Molecular & Biochemical Parasitology 160 (2008) 171174 Contents lists available at ScienceDirect
- Introduction Many eukaryotic cell surface proteins, including receptors,
- Available online at www.sciencedirect.com Molecular & Biochemical Parasitology 157 (2008) 7382
- (triplicates) Cell # -SM 2.0 0.035* (-) control
- Molecular and Biochemical Parasitology 99 (1999) 89101 A tightly regulated inducible expression system for conditional
- The architecture of variant surface glycoprotein gene expression sites in Trypanosoma brucei
- Tools for genetic analysis in Trypanosoma brucei Last updated 4 December 2008
- Parasitology (1973), 67, 315-331 With 6 figures in the text
- Molecular and Biochemical Parasitology 103 (1999) 3548 Conditional expression of glycosylphosphatidylinositol
- SDS-PAGE PROTOCOL Adapted from Current Protocols, Ch. 10
- 7/9/10 2:34 PMWHO | Human African trypanosomiasis: number of new cases drops to historically low level in 50 years Page 1 of 2http://www.who.int/neglected_diseases/integrated_media/integrated_media_hat_june_2010/en/index.html
- Affinity purification of antibodies Additional materials
- Genomic and episomal DNA isolation protocols Large scale using DNAzolTM
- Preparation of electro-competent DH10B cells The day before inoculation, prepare following
- Electrophoretic Mobility Shift Assay (EMSA: gel shift) for VSG ES promoter
- Luciferase and -galactosidase assays in procyclic forms Transient transfection
- Rotating Field Gel Electrophoresis (RAGE) is a variation on Pulsed Field Gel Electrophoresis (PFGE) and gives similar results. We use equipment that was only briefly marketed by
- Transfection of T. brucei Lister 427 and its derivatives PLEASE NOTE: The Amaxa Nucleofector transfection technology, which we have been using since
- RT-PCR cloning of expressed VSGs Harvesting cells
- Growth of bloodstream and procyclic T. brucei colonies on agarose plates Vern B. Carruthers & Kevin Tan, Laboratory of Molecular Parasitology, Rockefeller University.
- Proc. Nati. Acad. Sci. USA Vol. 89, pp. 8818-8821, September 1992
- MOLECULAR AND CELLULAR BIOLOGY, July 2006, p. 49114919 Vol. 26, No. 13 0270-7306/06/$08.00 0 doi:10.1128/MCB.00212-06
- Molecular Cell 23, 497507, August 18, 2006 2006 Elsevier Inc. DOI 10.1016/j.molcel.2006.06.027 Selective Di-or Trimethylation of Histone H3
- MOLECULAR AND CELLULAR BIOLOGY, Nov. 2005, p. 95869594 Vol. 25, No. 21 0270-7306/05/$08.00 0 doi:10.1128/MCB.25.21.95869594.2005
- An organism-specic method to rank predicted coding regions in Trypanosoma brucei
- Molecular & Biochemical Parasitology 128 (2003) 135145 Structural features affecting variant surface glycoprotein
- Trypanosoma brucei MRE11 is non-essential but influences growth, homologous recombination and DNA double-strand break repair
- Molecular & Biochemical Parasitology 117 (2001) 211215 Short communication
- Articledoi:10.1006/geno.2001.6616, available online at http://www.idealibrary.com on IDEAL INTRODUCTION
- Molecular and Biochemical Parasitology 111 (2000) 309317 Genetic manipulation indicates that ARD1 is an essential
- Molecular and Biochemical Parasitology 94 (1998) 5366 In situ analysis of a variant surface glycoprotein expression-site
- Molecular and Biochemical Parasitology 84 (1997) 189201 Analysis of Trypanosoma brucei 6sg expression site switching
- IF of living cells (in suspension) for microscopy/FACS Starting material > 106
- Parasite Dot-Blot for analysis of VSG expression This protocol is a derivative of the classical Western blot.
- Continuous Cultivation of Trypanosoma brucei Blood Stream Forms in a Medium Containing a Low Concentration of Serum Protein without Feeder Cell Layers
- Molecular & Biochemical Parasitology 114 (2001) 245247 Short communication
- Nicolai Siegel Page 1 updated: 6/4/09 CHIP-Seq protocol
- Telomere length regulation and transcriptional silencing in KU80-deficient Trypanosoma brucei
- Trypanosoma brucei expression-site-associated-gene-8 protein interacts with a Pumilio family protein
- EMSA for T. brucei TRF Gel preparation
- media preparation protocols Revised on 5/23/08 Trypanosome Media Preparation Protocols
- Introduction The structure and function of a nucleosome can be dramatically
- Molecular & Biochemical Parasitology 149 (2006) 102107 Short communication
- Transfection of bloodstream T. brucei Lister 427 using the AMAXA Nucleofector apparatus
