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Soderling, Scott - Department of Cell Biology, Duke University
Updated 1/24/05 Calcium Phosphate Transfection page 1 of 1 Calcium Phosphate Transfection
Updated 2/11/05 Expression and Purification of GST-Fusion Proteins page 1 of 2 v. II (Procedure Version 2)
Lab Recipes 1 liter TTBS (aka TBST)
Updated 10/11/05 Preparation and Transformation of Highly Efficient page 1 of 3 Chemically Competent E. coli
Short TechnicalReports Two-Stage PCR Protocol
Lipofectamine 2000 transfect neurons: For 24 well plate
Updated 3/28/05 Affinity Purification of Antibody From Antiserum page 1 of 2 Affinity Purification of Antibody From Antiserum
Soderling Lab Recombineering Protocol (Copied from Liu, P., et.al., Genome Research, 13:476-484; 2003 with some changes.)
Simultaneous Introduction of Multiple Mutations
