- Transfection of [T47D] Cells Day 1 (8/20/02)
- Iruela-Arispe Lab University of California, Los Angeles
- Iruela-Arispe Lab University of California, Los Angeles
- Iruela-Arispe Lab University of California, Los Angeles
- Cutaneous Wound Healing Assay (from Monika Krampert) 1. anesthetize the mice, shave the back and wipe it with ethanol
- Modified Matrigel Assay Lee, Sunyoung
- Protocol for isolation of Endothelial cells from embryos 1) Use sterile conditions; autoclave forceps. Work in hood.
- Protocol for LacZ Staining: List of Reagents
- KDR Phosphorylation Assay 2-17-06 Lee, Sunyoung
- Whole mount In Situ Hybridization protocol All reagents prior to hybridization should be RNASE free: Use DEPC treated ddH2O for
- LaserSharp2000 Confocal Operations (Adapted from BioRad LaserSharp 2000 Software User Guide)
- Iruela-Arispe Lab University of California, Los Angeles
- Iruela-Arispe Lab University of California, Los Angeles
- Protocol for isolation of EC from adult liver/heart (AL) (Protocol originally from Jackie) Lee, Sunyoung
- Updated on 11/13/2008 STAINING PROTOCOL FOR FACS
- Protocol for evaluating vascular permeability in wildtype and PRTg mice
- 25 ml 10 ml Xgal wash buffer 24.1 ml 9.64 ml
- Arnaud Monvoisin/mp/cb Bone Marrow Transplantation Protocol
- OVARIAN TRANSPLANT (Need dissecting scope) Use FVB/N x black or agouti F1 hybrids as recipients. Not only are hybrids better mothers that inbred strain
- The DLAM pharmacy does not normally keep PMSG and hCG stocked. However, I have aliquots (each aliquot is enough for 10 mice) of PMSG and hCG that I can bring over from the rederivation
- Ruchi Shrivastava, Winter 2004 Metabolic Labeling and Immunoprecipitation of VEGF Isoforms
- Protocol for Zymogram 2-17-06 Lee, Sunyoung
- Lee, Sunyoung VEGF Western on Tricine Gel
- PCR genotyping for ADAMTS1 KO mice Stephanie Lauw
- Iruela-Arispe Lab University of California, Los Angeles
- Citrate Buffer Epitope Retrieval Method Description: Formalin or other aldehyde fixation forms protein cross-links that
- IHC CD31 PARAFFIN SECTIONS 6. 1X PBS 5'
- Immunocytochemistry for Myosin Light Chain For Cells in Suspension
- Reverse Transcription Taqman RT-PCR reagents (Applied Biosystems) were used for the generation of cDNA for all samples.
- Reverse Transcription for Standard RNA (AB Taqman) Total RNA (best from liver) (2.0 g) Xl
- Iruela-Arispe Lab University of California, Los Angeles
- *dioctadecyl-6,6'-di(4-sulfophenyl)-3,3,3',3'-tetramethylindocarbocyanine (Molecular Probes)
- *dioctadecyl-6,6'-di(4-sulfophenyl)-3,3,3',3'-tetramethylindocarbocyanine (Molecular Probes)
- Iruela-Arispe Lab University of California, Los Angeles
- ADAMTS-1 purification 1. Growth cells expressing ADAMTS-1 in 150 mm plate with DMEM 10% FBS or FCS.
- Created by Tom Chen Last printed 11/14/08 3:51 PM -1 -
- ISOLATION OF ENDOTHELIAL CELLS FROM MOUSE EMBRYOS Pregnant mouse is sacrificed by cervical dislocation 9.5dpc, the uterus is removed
- Media Recipes A. Zovein 4-3-04 MAEC media for Fetal liver
- S-Methionine Metabolic Labeling 1. Seed cells (250,000 cells/well for 293T) in 6-well plate. Put each time point on
- Immunocytochemistry Prep: -Warm several hundred mls of 1X PBS to 37C
- Matrigel (BD Biosciences, Fisher cat# 354234)(maybe less $ directly from BD) DMEM (low glucose) with D-Valine (Specialty Media cat#: D-087-B)
- Isolating endothelial cells from bovine aortas Sterilize scissors and forceps the night before by autoclaving.
- Tomoatsu Kaneko & Elisabeta Karl (Nor's lab) Lentiviral Infection of Endothelial Cells (HDMEC)
- Shell-less embryo culture Fertilized white leghorn chicken eggs (SPAFAS Inc., Norwich, CT) were received at day 0 and
- General Matrigel Angiogenesis Assay Each animal will have two Matrigel implants of 250ul each. One with Matrigel/heparin and the
- Labeling Cells with Live Dyes This protocol provides a way to stain live cells by using the following three dyes from
- Iodination and Injection of 125 I. Column Preparation (Sephadex G-25 Column)
- Iruela-Arispe Lab University of California, Los Angeles
- Iruela-Arispe Lab University of California, Los Angeles
- BONE MARROW TRANSPLANTATION (adapted from Lusis lab protocol and Arnaud Monvoisin)
- DNA Genotyping Protocol A. Zovein Lysis Buffer
- Iruela-Arispe Lab University of California, Los Angeles
- Iruela-Arispe Lab University of California, Los Angeles
- Polyoma middle T PRODUCTION FROM THE PACKAGING CELL LINE Polyoma middle T PRODUCTION
- Iruela-Arispe Lab University of California, Los Angeles
- For research use only; not for use as a diagnostic. 2003-2005: CHEMICON International, Inc. -By CHEMICON International, Inc. All rights reserved. No part of these works may be reproduced in any form without permissions in writing.
- Whole Mount Retina IHC 1. Fix eyes in 4% PFA for 45-60
- PCR genotyping ADAMTS1 KO mice Stephanie Lauw Reaction Mix
- Protocol for LacZ Staining (mouse VR26): Stock: 0.5M EGTA pH8.0
