- 34. Cortright, R. N., Collins, H. L., Rodenbaugh, D. W. & DiCarlo, S. E. Student retention of course content is
- Leading Edge Cell 132, January 25, 2008 2008 Elsevier Inc. 185
- Embryonic Dorsal-Ventral Signaling: Secreted Frizzled-Related Proteins
- Developmental Expression Pattern Identification of a second Xenopus Twisted Gastrulation gene
- Crossveinless-2 is required for the relocalization of Chordin protein within the vertebral field in mouse embryos
- Development of the vertebral morphogenetic field in the mouse: Interactions between Crossveinless-2 and Twisted Gastrulation
- Introduction Bone Morphogenetic Proteins (BMPs) are growth factors
- Mad Is Required for Wingless Signaling in Wing Development and Segment Patterning in Drosophila
- The opposing homeobox genes Goosecoid and Vent1/2 self-regulate Xenopus patterning
- Animal Cap Assay (AC assay) -Hiroki Kuroda A Basic Technique for Embryo Manipulation
- BDA lineage tracing on sections -Hiroki Kuroda Kuroda et al., PLoS 2, 0623-0634 (2004)
- Dissociation-and-reaggregation of animal cap cells Hiroki Kuroda Kuroda et al. Genes Dev. 19, 1022-1027 (2005)
- In situ hybridization on Sections -Hiroki Kuroda Note. Embed sample in paraffin in HE staining case.
- In Situ Hybridization to detect miRNA expression in Xenopus using Locked Nucleic Acid Probes
- Keller sandwich explant -Hiroki Kuroda Kuroda et al., PLoS 2, 0623-0634 (2004)
- Natural and Artificial Fertilization of Frog Eggs -Hiroki Kuroda A) Natural Fertilization of Frog Eggs
- Northern Blot -Hiroki Kuroda Sometimes useful to check amount and size of RNA
- Western Blot for phosphoprotein samples -Lucho Fuentealba (Samples of phosphorylated proteins)
- Alcian Blue Alizarin Red Skeletal Staining October 2003
- Whole Mount In Situ Hybridization On Mouse Embryos
- Fly whole mount immunostaining Peggy Vorwald and Ed Eivers 2008
- Drosophila Primers for Quantitative RT-PCR Veronika Sander 2009
- Dual Luciferase Assay Lucho Fuentealba 2008
- Synchronization of Cell Division Lucho Fuentealba 2008
- Wnt Signaling Requires Sequestration of Glycogen Synthase Kinase 3
- Drosophila Smad2 Opposes Mad Signaling during Wing Vein Development
- How do these cells die? They perish through a specialized form
- Integration of BMP and Wnt signaling via vertebrate Smad1/5/8 and Drosophila Mad
- COGEDE-509; NO OF PAGES 7 Please cite this article in press as: Eivers E, et al., Integrating positional information at the level of Smad1/5/8, Curr Opin Genet Dev (2008), doi:10.1016/j.gde.2008.06.001
- ARTICLE IN PRESS xBtg-x regulates Wnt/h-Catenin signaling during early
- Original Article Exploration of the extracellular space by a large-scale
- Neural Induction in Xenopus: Requirement for Ectodermal and Endomesodermal Signals
- Annu. Rev. Cell Dev. Biol. 2004. 20:285308 doi: 10.1146/annurev.cellbio.20.011403.154124
- Developmental Cell, Vol. 4, 219230, February, 2003, Copyright 2003 by Cell Press Chordin Is Required for the Spemann Organizer
- Gene expression pattern Neuralin-1 is a novel Chordin-related molecule expressed in the mouse
- Gene expression pattern Identication and expression of the mammalian homologue of Bicaudal-C
- In 1924 an article appeared in a German scientific journal describing the most important experiment in all the history of Embryology and probably one of the most influential pieces of research in the whole field of Biology.
- 2001 European Molecular Biology Organization EMBO reports vol. 2 | no. 8 | pp 661665 | 2001 661 New twists on embryonic patterning
- Mallory's Tetrachrome Staining Of Mouse Sections Lise Zakin 2008
- Neural Induction in the Absence of Mesoderm: -Catenin-Dependent Expression of Secreted BMP
- Xenopus Whole Mount In-Situ Hybridization Protocol Jack Greenan and Carrie Metzinger 2008
- The molecular ancestry of segmentation mechanisms E. M. De Robertis1
- Neuron, Vol. 33, 489494, February 14, 2002, Copyright 2002 by Cell Press tif of as yet unknown function is found in manyNeural Plate Patterning
- RNA extraction, cDNA synthesis and Quantitative PCR for Xenopus and Drosophila samples
- Darmin is a novel secreted protein expressed during endoderm development in Xenopus
- Cell Immunostaining Lucho Fuentealba 2008
- Activity-Induced Protocadherin Arcadlin Regulates Dendritic Spine Number by Triggering N-Cadherin
- RT-PCR Hiroki Kuroda/Hojoon Lee Most Common Technique for detecting molecular markers
- Spemann's organizer transplantation -Hiroki Kuroda Note: All surgery steps are done with forceps.
- Introduction Sirenomelia is a human developmental malformation that has
- Enzymatic regulation of pattern: BMP4 binds CUB domains of Tolloids
- Analysis of Spemann organizer formation in Xenopus embryos by cDNA macroarrays
- Regulation of ADMP and BMP2/4/7 at Opposite Embryonic Poles Generates a
- In Vitro Fertilization of Xenopus Laevis Frog Eggs Doug Geissert and Eddy De Robertis 2009
- INTRODUCTION Dorsoventral patterning in vertebrates and invertebrates is
- List of RT-PCR primers of the De Robertis laboratory all PCR reactions were performed using the following cycle conditions: 95C denaturation, 55C annealing, 72C elongation
- Secreted antagonists pattern the gastrula 189 Molecular mechanisms of cell-cell signaling by
- Cuticle Preparations Ed Eivers 2008
- Xenopus Whole Mount Immunostaining David Greenberg, Lucho Fuentealba, and Eddy M. De Robertis
- Introduction Growth factors of the bone morphogenetic protein (Bmp)
- Expression of Siamois and Twin in the blastula Chordin/Noggin signaling center is required for brain formation in Xenopus
- Mouse Crossveinless-2 is the vertebrate homolog of a Drosophila extracellular regulator of BMP signaling
- Gene expression pattern Isthmin is a novel secreted protein expressed as part of the Fgf-8
- Chordin-like CR domains and the regulation of evolutionarily conserved extracellular signaling systems
- Systems biology of embryonic morphogens Jean-Louis Plouhinec and E. M. De Robertis*
- In Situ Hybridization On Mouse Cryostat sections Modified by Lise Zakin and Eddy De Robertis 2008
- 1107RESEARCH ARTICLE INTRODUCTION
- Integrating Patterning Signals: Wnt/GSK3 Regulates the Duration
- scientificreport 2003 EUROPEAN MOLECULAR BIOLOGY ORGANIZATION EMBO reports VOL 4 | NO 8 | 2003
- Paraxial protocadherin coordinates cell polarity during convergent extension via Rho A and JNK
- INTRODUCTION The establishment of the vertebrate body plan requires
- OBTAINING OOCYTES FROM FROGS BY SURGERY Thomas Brglin, Michael Mueller and Eddy De Robertis
- RESEARCH COMMUNICATION Integration of IGF, FGF, and
- Current Biology Vol 18 No 8 bees at two sites where hives were
- Overexpression of the Xenopus Tight-Junction Protein Claudin Causes Randomization
- Available online at www.sciencedirect.com Integrating positional information at the level of Smad1/5/8
- brief communications NATURE CELL BIOLOGY ADVANCE ONLINE PUBLICATION http://cellbio.nature.com 1
- Developmental Cell, Vol. 1, 655665, November, 2001, Copyright 2001 by Cell Press Neural and Head Induction
- Spemann's organizer and the self-regulation of embryonic fields
- Developmental Cell Crossveinless-2 Is a BMP Feedback Inhibitor
- Immunohistochemistry on Mouse Sections Carrie Metzinger and Ellen Chang 2009
- INTRODUCTION Dorsoventral patterning in developing embryos is established
- Alcian Blue Cartilage Staining Beatrice Jegalian & Eddy M. De Robertis
- INTRODUCTION One of the most influential experiments in amphibian
- With its rapid embryonic development, large egg size (12 mm in diameter) and high numbers of embryos
- Hematoxylin & Eosin (HE) staining -Hiroki Kuroda (Sectioning)
- Neural induction in the absence of organizer in salamanders is mediated by MAPK
- In situ hybridization on mouse paraffin sections Modified by Ellen Chang 2009
- Wholemount immunostaining with Alkaline Phosphatase Antibodies Hiroki Kuroda
- Pulse and chase labeling with [35S]-Methionine Lucho Fuentealba 2008
- RESEARCH COMMUNICATION Default neural induction
- Asymmetric mitosis: Unequal segregation of proteins destined for degradation
- NATURE |VOL 405 |15 JUNE 2000 |www.nature.com 757 The evolutionarily conserved
- Xenopus laevis Morpholinos De Robertis Lab
- Wnt Signaling Requires Sequestration of Glycogen Synthase Kinase 3
- DorsalVentral patterning: Crescent is a dorsally secreted Frizzled-related protein that competitively inhibits Tolloid proteases
- Temporal pattern of the posterior expression of Wingless in Drosophila blastoderm Peggy P. Vorwald-Denholtz, Edward M. De Robertis
- COGEDE-840; NO. OF PAGES 8 Please cite this article in press as: Plouhinec J-L, et al. Systems control of BMP morphogen flow in vertebrate embryos, Curr Opin Genet Dev (2011), doi:10.1016/j.gde.2011.09.001
- D E V E L O P M E N T A L B I O L O G Y Phosphorylation of Mad Controls Competition
