- The Arabidopsis AAA ATPase SKD1 Is Involved in Multivesicular Endosome Function and Interacts with Its
- University Buildings 1. Administrative and Research Center--East Campus (J-2)
- High-efficiency transformation of yeast with a plasmid library*** 1. Inoculate 100 mL YPD with an overnight culture of cells so that the final
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- Preparation of genomic DNA for PCR 1. Centrifuge 5 mL of a saturated overnight culture of yeast cells at 5000 x g for 5 min at RT.
- Molecular mechanisms of late endosome morphology, identity and sorting
- Dual mechanisms specify Doa4-mediated deubiquitination at multivesicular bodies
- FM 4-64 labeling of yeast vacuole membranes FM 4-64 is a lipophilic styryl dye sold by Molecular Probes (product no. T-3166;
- Developmental Cell, Vol. 8, 937947, June, 2005, Copyright 2005 by Elsevier Inc. DOI 10.1016/j.devcel.2005.04.001 Structural Basis for Endosomal Targeting
- Prepared by Greg Odorizzi Last updated 11/16/03 Preparation of Yeast Spheroplasts
- Yeast Genomic Library Genomic DNA Sau3AI partial digestion
- Plasmid DNA minipreps (alkaline lysis method) 1. Centrifuge 1 mL of overnight bacterial culture in eppi tube for 2 min at 10,000 x g
- Molecular Biology of the Cell Vol. 21, 000000, March 15, 2010
- Author's personal copy Available online at www.sciencedirect.com
- Molecular Biology of the Cell Vol. 18, 697706, February 2007
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- TheJournalofCellBiology The Rockefeller University Press, 0021-9525/2004/08/717/13 $8.00
- TOPO-cloning of PCR products 1. Perform PCR
- Preparation of competent bacteria -Inoue method Day 1: Streak bacteria from frozen stock onto LB agar medium; incubate O/N at 37C
- Pulldown of GST fusion mixed with extract of BL21(DE3)/codon+ expressing protein of interest The following protocol is for mixing a bacterial lysate with up to 4 GST proteins; scale accordingly
- Transformation of Yeast 1. Grow cells in liquid culture to mid-logarithmic stage (0.5-0.8 ODU/ml)
- Glass bead lysis of whole yeast cells The following protocol is suitable for preparation of 5 OD600 units of whole-cell extract at a final
- Immunofluorescence Microscopy of Yeast A. Spheroplasting Cells
- EMBO reports VOL 8 | NO 7 | 2007 2007 EUROPEAN MOLECULAR BIOLOGY ORGANIZATION644 conceptconcept
