- Isolation of total DNA (Goyon and Lichten (1993) Mol. Cell. Biol. 13, 373-382. -take 40 ODU cells (e.g. 40 ml of OD600=1), optional: fix in 50 % ethanol at
- The DNA damage checkpoint pathways exert multiple controls on the efficiency and outcome of the repair
- YEAST VOL. 13: 15351545 (1997) Green Fluorescent Protein as a Reporter for the DNA
- Miniprep (alkaline lysis) -solutions: -solution 1: 50 mM glucose
- Dephosphorylation of Plasmid DNA During ligation in vitro, T4 DNA ligase will catalyze the formation of a
- MOLECULAR AND CELLULAR BIOLOGY, 0270-7306/99/$04.00 0
- Who's who in human recombination: BRCA2 and RAD52 and Wolf-Dietrich Heyera,b,1
- Protein gel electrophoresis (Lmmli) -solutions: -Tris gel buffer: 375 mM Tris pH 8.8
- Denaturing purification of Insoluble Proteins Buffer A 500 ml 1 L
- Western Blot (wet transfer) -solution: -transfer buffer: 12.1 g Tris base
- Alternate pathways involving Sgs1 Top3, Mus81 Mms4, and Srs2 prevent formation of toxic
- DNA Repair 3 (2004) 769776 The Hog1 MAP kinase pathway and the Mec1 DNA damage
- Blunt-ended Ligation The maximum number of "correct" clones can generally be obtained from ligation
- Molecular Cell, Vol. 10, 11751188, November, 2002, Copyright 2002 by Cell Press Rad54, a Swi2/Snf2-like Recombinational Repair
- ATP/NADH coupled assay for Rad54 ATPase The assay is based on a reaction in which the regeneration of hydrolyzed ATP is
- MOLECULAR AND CELLULAR BIOLOGY, Feb. 2003, p. 14411452 Vol. 23, No. 4 0270-7306/03/$08.00 0 DOI: 10.1128/MCB.23.4.14411452.2003
- Rad54 Protein Exerts Diverse Modes of ATPase Activity on Duplex DNA Partially and Fully Covered with Rad51 Protein*
- Media for S. cereviseae -YPD (yeast extract peptone dextrose)
- Yeast 15, 721740 (1999) Specific Negative Effects Resulting from Elevated Levels
- DNA Repair 2 (2003) 10411064 Amino acid changes in Xrs2p, Dun1p, and Rfa2p that remove the
- E. coli competent cells and transformation (Inoue et al. 1990 Gene 96, 23-28) How to prepare TB buffer?
- DNA EXTRACTION USING CTAB 1. A saturated ( 1.6 x 10e9 cells in 8 ml) overnight culture was pelleted at 3000g for
- How to handle MMS ! MMS (=Methyl-methansulfonate, CH3SO3CH3, Mr 110.13) is used in the
- Top Curr Genet Springer-Verlag Berlin Heidelberg 2006
- Two-dimensional Analysis of Meiotic Recombination Intermediates at HIS4LEU2 1. Day 1. Digest 2g DNA to completion. Typically 2 g in 80 L final volume with
- www.cell-research.com | Cell Research Xuan Li and Wolf-Dietrich Heyer
- For general laboratory use. FOR IN VITRO USE ONLY.
- For use if you have compatible sticky ends. Buffers and Solutions
- Recombination: Holliday Junction Resolution and Crossover Formation
- Psoralen=Guanidine DNA preparation 1. To15 mL meiotic sample (~6 x 108
- RESEARCH ARTICLE Lindsey Walsh Jacqueline Schmuckli-Maurer
- Running a CHEF Gel (S. cerevisiae full genome spread) 1) Acquire gel casting tray and comb.
- Molecular Cell, Vol. 6, 583592, September, 2000, Copyright 2000 by Cell Press Rad54 Protein Is Targeted to Pairing Loci
- Rad54 Protein Stimulates Heteroduplex DNA Formation in the Synaptic Phase of DNA Strand
- Molecular Cell, Volume 36 Supplemental Data
- How to archive strains and plasmids? All yeast and E.coli strains and plasmids that are received or constructed in
- Terminal association of Rad54 protein with the Rad51dsDNA filament
- ORIGINAL PAPER H. Interthal W.-D. Heyer
- Restriction digest: 2 U restriction enzyme
- Notes & Tips NADH-coupled microplate photometric assay for kinetic studies of
- ORIGINAL PAPER J. Schmuckli-Maurer W.-D. Heyer
- New Cross-linking Protocol (1 X-link/50-60bp) 1. Harvest approx. 6 x 108
- DNA Polyacrylamide Gel Electrophoresis How to pour and run a neutral polyacrylamide gel.
- [10] DNA DamageInduced Phosphorylation of Rad55 Protein as a Sentinel for DNA Damage Checkpoint
- The Rockefeller University Press, 0021-9525/97/02/761/13 $2.00 The Journal of Cell Biology, Volume 136, Number 4, February 24, 1997 761773 761
- Copyright 1999 by the Genetics Society of America A New Recombinational DNA Repair Gene From Schizosaccharomyces pombe
- Sporulation of S.cerevisiae Production of diploid strains
- Molecular Cell Short Article
- 638646 Nucleic Acids Research, 2009, Vol. 37, No. 2 Published online 11 December 2008 doi:10.1093/nar/gkn980
- Regulation of Homologous Recombination in Eukaryotes
- nature structural & molecular biology advance online publication 1 Human BRCA2 protein promotes
- A truncated DNA-damage-signaling response is activated after DSB formation in the G1 phase
- 20262036 Nucleic Acids Research, 2009, Vol. 37, No. 6 Published online 11 February 2009 doi:10.1093/nar/gkp038
- Revised supplemental data Zhang et al. 1 Supplemental data for Zhang et al.
- Genome Dyn Stab (3) R. Egel, D.-H. Lankenau: Recombination and Meiosis
- 21822195 Nucleic Acids Research, 2008, Vol. 36, No. 7 Published online 16 February 2008 doi:10.1093/nar/gkm1152
- Supplemental Materials SM Figure 1. Genomic Southern blot analysis of MUS81 in rice. Total DNA (10 g each)
- MOLECULAR AND CELLULAR BIOLOGY, Nov. 2006, p. 83968409 Vol. 26, No. 22 0270-7306/06/$08.00 0 doi:10.1128/MCB.01317-06
- dna repair 5 ( 2 0 0 6 ) 618628 available at www.sciencedirect.com
- Molecular Cell, Vol. 16, 19, October 8, 2004, Copyright 2004 by Cell Press Meeting ReviewRecombination Mechanisms
- Damage Signaling: RecQ Sends an Wolf-Dietrich Heyer
- Genome instability in rad54 mutants of Saccharomyces cerevisiae
- Holliday junctions in the eukaryotic nucleus: resolution in sight?
- Rad54 protein stimulates the postsynaptic phase of Rad51 protein-mediated DNA strand exchange
- DNA repair: CPR for the DNA Damage and Repair: Vol. III Advances
- Bioelectromagnetics 21:515^523 (2000) No Mutagenic or Recombinogenic Effects of
- Biosensors & Bioelectronics 13 (1998) 831838 Development of a green fluorescent protein reporter for a yeast
- Bioelectromagnetics 18:142155 (1997) Extremely High Frequency
- S.10 1357-4817/98/$ see front matter. 1998 Published by Elsevier Science Ltd. All rights reserved. Genetic Nomenclature Guide 1998 SACCHAROMYCES CEREVISIAE OVERVIEW EXAMPLES
- PCR-based gene disruption in Saccharomyces cerevisiae using SFH-or LFH-PCR and kanMX selectable marker
- Yeast Genomic DNA Prep ("10-minute protocol")
- Restriction analysis of plasmids -Solutions: -1x TBE: make from 10xTBE
- CHEF GEL WITH EMBEDDED YEAST DNA 1) Inoculate a single colony into 50 ml YPD. Grow in shaker to an OD600 of > 1.0
- IMMUNOPRECIPITATION (IP) 1. Grow cells up to OD600=1.0 (for haploid 1n strain), or =2.0 (for 2n
- DNA strand exchange reaction -solutions: -5x strand exchange buffer
- Nucleoprotein gel assay for DNA-Rad51 complexes The assay is based on the difference in electrophoretic mobility of glutaraldehyde-fixed DNA-
- Abstract. The Rad54 protein is an important component of the recombinational DNA repair pathway in vegeta-
- MOLECULAR AND CELLULAR BIOLOGY, 0270-7306/00/$04.00 0
- The EMBO Journal Vol.16 No.9 pp.25352544, 1997 Recombinational repair in yeast: functional
- ORIGINAL PAPER Abstract The Schizosaccharomyces pombe rhp51+
- MOLECULAR AND CELLULAR BIOLOGY, 0270-7306/00/$04.00 0
- 10.1101/gad.1477106Access the most recent version at doi: 2006 20: 2479-2486Genes & Dev.
- Short Communication Two Alternatively Spliced Transcripts Generated from OsMUS81, a Rice
- Galactose induction for analytical purposes -solutions: -5x buffer A: 0.1 M Tris pH 7.5
- Yeast Transformation Protocol (~ 300,000 transformants/microgram DNA) 1-Start a 50 ml YPD culture at a 1:100 dilution from o/n culture, grow at 30C until
- Functions of the Snf2/Swi2 family Rad54 motor protein in homologous recombination
- LETTER doi:10.1038/nature10522 Rad51 paralogues Rad55Rad57 balance the
