- Immunoprecipitations 1. Use 300-400 g protein per IP
- Targeting the Dimerization of Epidermal Growth Factor Receptors with Small-Molecule Inhibitors
- SDS Polyacrylamide Gel Electrophoresis Gel Recipes
- Functional Characterization of an Epidermal Growth Factor Receptor/RET Chimera*
- PC PE PS PI SPM GangChol
- Triton X-100 Caveolae Prep Solutions needed
- ASP-960/GLU-961 CONTROL THE MOVEMENT OF THE C-TERMINAL TAIL OF THE EGF RECEPTOR TO REGULATE ASYMMETRIC DIMER FORMATION
- Palmitoylation of the EGF Receptor Impairs Signal Transduction and Abolishes High-Affinity Ligand Binding
- In Vivo PI Turnover Assay Labeling Cells and Assaying
- Cell Growth on Soft Agar Plates Buffers Needed
- Incorporation of Sterols into Cells Using Cholesterol/Cyclodextrin Complexes
- Smart Prep of Caveolae (Smart, E.J., Ying, Y.-S., Mineo, C. and Anderson, R.G.W. (1995) A Detergent-Free Method for
- Neutral Lipid Extraction by the Method of Bligh-Dyer (Bligh and Dyer, Can. J. Biochem. Physiol. 37, 922 (1959))
- Labeling Cells and Analyzing Inositol Phospholipids Reagents Required
- Making Tissue Culture Medium 1. Autoclave either 12 one-liter or 20 half-liter bottles.
- Freezing Cells Make sure cells are in log phase of growth, ie. subconfluent
- EGF Receptor Purification from A431 Cells Solutions/Materials Needed
- Copyright 2005 by the American Society for Biochemistry and Molecular Biology, Inc. This article is available online at http://www.jlr.org Journal of Lipid Research Volume 46, 2005 1061
- Modulation of nucleotide sensitivity of ATP-sensitive potassium channels by phosphatidylinositol-4-
- The challenge of lipid rafts Linda J. Pike1
- Iodination of EGF 1. 1 mCi 125
- 125I-EGF Binding to Whole Cells Solutions Needed
- Cholesterol Depletion Results in Site-specific Increases in Epidermal Growth Factor Receptor Phosphorylation due to
- Western Blotting A. Electrophoretic transfer of proteins to nitrocellulose
- Arg-Arg-Src Peptide Kinase Activity Assay For Tyrosine Kinases
- Rafts defined: a report on the Keystone symposium on lipid rafts and cell function
- Dynamic Sensitivity of ATP-sensitive K Channels to ATP* Received for publication, March 16, 2001, and in revised form, April 17, 2001
- Biochem. J. (2004) 378, 281292 (Printed in Great Britain) 281 REVIEW ARTICLE
- Determination of Cellular Cholesterol Content Reagents Needed
- Luciferase Fragment Complementation Imaging of Conformational Changes in the Epidermal Growth
- Localization and Turnover of Phosphatidylinositol 4,5-
- Oligomerization of the EGF Receptor Investigated by Live Cell Fluorescence Intensity Distribution Analysis
- Making Caveolae by the Alkaline Detergent-Free Procedure (modified from Song et al. (1996) J. Biol. Chem. 271:9690-9697)
- Cholesterol Depletion Delocalizes Phosphatidylinositol Bisphosphate and Inhibits Hormone-stimulated
- The Intracellular Juxtamembrane Domain of the Epidermal Growth Factor (EGF) Receptor Is Responsible for the
- Plasma Membrane Phospholipid Scramblase 1 Is Enriched in Lipid Rafts and Interacts with the Epidermal Growth Factor Receptor
- Phosphoamino Acid Analysis of 32 P-Labeled Proteins
- Detergent-Free Caveolae Prep (from: Liu, Casey and Pike, BBRC 245: 684-690 (1998)
- Lipid Rafts Are Enriched in Arachidonic Acid and Plasmenylethanolamine and Their Composition Is Independent of Caveolin-1 Expression: A Quantitative
- Precipitation Lowry Protein Assay (Peterson, Anal. Biochem. 83, 346 (1977))
- Making Hanks' Balanced Salt Solution 1. Autoclave either 12 one-liter or 20 half-liter bottles.
- The membrane proximal disulfides of the EGF receptor extracellular domain are required for high affinity binding and signal transduction but do not
- RIPA Lysates of Cells Solutions Needed
- Heterogeneity in EGF-binding affinities arises from negative cooperativity in an aggregating system
- Copyright 2003 by Lipid Research, Inc. This article is available online at http://www.jlr.org Journal of Lipid Research Volume 44, 2003 655
- Correlating EGFR Expression with Receptor-Binding Properties and Internalization of
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 245, 684690 (1998) ARTICLE NO. RC988329
- Splitting Cells For a 150 mm dish of 3T3 cells
- In Vitro EGF Receptor Autophosphorylation Buffers Needed
- THE TETHERING ARM OF THE EGF RECEPTOR IS REQUIRED FOR NEGATIVE COOPERATIVITY AND SIGNAL TRANSDUCTION
- Cholesterol Levels Modulate EGF Receptor-Mediated Signaling by Altering Receptor Function and Trafficking
