Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

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Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

NATURE BIOTECHNOLOGY VOL 17 NOVEMBER 1999 http://biotech.nature.com 1109 Conventional fluorescence-activated cell sorters (FACSs) are widely
Engineered bidirectional communication mediates a consensus in a microbial biofilm consortium
Accelerated Articles An Integrated Microfabricated Cell Sorter
Library analysis of SCHEMA-guided protein recombination
Advances in Complex Systems, Vol. 6, No. 1 (2003) 19 c World Scientific Publishing Company
Engineering microbial consortia: a new frontier in synthetic biology
Synthetic Gene Circuits: Design with Directed
In the light of directed evolution: Pathways of adaptive protein evolution
Computational method to reduce the search space for directed protein evolution
r XXXX American Chemical Society A dx.doi.org/10.1021/ja107936d |J. Am. Chem. Soc. XXXX, XXX, 000000 COMMUNICATION
JOURNAL OF BACTERIOLOGY, Dec. 2002, p. 66906699 Vol. 184, No. 23 0021-9193/02/$04.00 0 DOI: 10.1128/JB.184.23.66906699.2002
Supporting Information for "Implications of Rewiring Bacterial Quorum Sensing"
subsequent O-to-N rearrangement via 1,4-addition of the serine nitrogen to the activated cyclohex-
A synthetic Escherichia coli predatorprey ecosystem Frederick K Balagadde1,2,5
Protein Dynamics in a Family of Laboratory Evolved Thermophilic Enzymes
Metabolomics is a rapidly growing field of study that has traditionally focused on map-
Preparation of Human Metabolites of Propranolol Using Laboratory-Evolved
Supporting Information Wiley-VCH 2007
UNIT 26.2Combinatorial Recombination of Gene Fragments to Construct a Library of
A diverse family of thermostable cytochrome P450s created by recombination of stabilizing fragments
Protein stability promotes evolvability Jesse D. Bloom*
The genome sequence of T. parva shows remarkable differences from the other apicom-
Site-directed protein recombination as a shortest-path problem
Chemo-enzymatic fluorination of unactivated organic compounds
Journal of Cellular Biochemistry Supplement 37:5863 (2001) Computationally Focusing the Directed Evolution
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb. 2011, p. 14361442 Vol. 77, No. 4 0099-2240/11/$12.00 doi:10.1128/AEM.01802-10
Efficient screening of fungal cellobiohydrolase class I enzymes for thermostabilizing sequence blocks by
264 VOLUME 28 NUMBER 3 MARCH 2010 nature biotechnology A rt i c l e s
In making his case for the role of natural selection in evolution, Darwin started by pointing to the enormous
DOI: 10.1002/chem.200900643 A Panel of Cytochrome P450 BM3 Variants to Produce Drug Metabolites
SCHEMA Recombination of a Fungal Cellulase Uncovers a
Available online at www.sciencedirect.com Directed enzyme evolution: climbing fitness peaks one amino acid
A family of thermostable fungal cellulases created by structure-guided recombination
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Jan. 2009, p. 337344 Vol. 75, No. 2 0099-2240/09/$08.00 0 doi:10.1128/AEM.01758-08
5 Diggle, S.P. et al. (2007) Evolutionary theory of bacterial quorum sensing: when is a signal not a signal? Philos. Trans. R. Soc. Lond. B
Evolutionary History of a Specialized P450 Propane Monooxygenase
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Jan. 2008, p. 437445 Vol. 74, No. 2 0099-2240/08/$08.00 0 doi:10.1128/AEM.01688-07
Directed Evolution DOI: 10.1002/anie.200702616
BioMed Central Page 1 of 20
Structure-guided SCHEMA recombination of distantly related b-lactamases
and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org The Author 2006. Published by Oxford University Press on behalf of the Society for Molecular Biology
Biosynthesis of novel carotenoid families based on unnatural carbon backbones: A model for diversification of natural product pathways
Protein-Surfactant Film Voltammetry of Wild-Type and Mutant Cytochrome P450 BM3
Chemoenzymatic elaboration of monosaccharides using engineered cytochrome P450BM3 demethylases
COMMUNICATION Consensus Protein Design without Phylogenetic Bias
Directed evolution of a genetic circuit Yohei Yokobayashi*, Ron Weiss
Long-term Monitoring of Bacteria Undergoing Programmed Population Control in a Microchemostat
DOI: 10.1002/cbic.201000565 Combinatorial Alanine Substitution Enables Rapid Optimization of
Recent in vitro recombination or "DNA shuf-fling" experiments have demonstrated that
COBIOT-658; NO OF PAGES 6 Please cite this article in press as: Dougherty MJ, Arnold FH. Directed evolution: new parts and optimized function, Curr Opin Biotechnol (2009), doi:10.1016/j.copbio.2009.08.005
COBIOT-866; NO. OF PAGES 9 Please cite this article in press as: Jung ST, et al. Cytochrome P450: taming a wild type enzyme, Curr Opin Biotechnol (2011), doi:10.1016/j.copbio.2011.02.008
Self-Organization, Layered Structure, and Aggregation Enhance Persistence of a Synthetic Biofilm Consortium
A structural study of Hypocrea jecorina Cel5A Journal: Protein Science
Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in
Available online at www.sciencedirect.com Optimizing non-natural protein function with directed evolution
The Library of Maynard-Smith: My Search for Meaning in the Protein
ChemBioChem 2003, 4, 891 893 DOI: 10.1002/cbic.200300660 2003 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim 891 Thermostabilization of a Cytochrome
Elastomeric Microfabricated Fluorescence-Activated Cell Sorters
750 NATURE BIOTECHNOLOGY VOL 18 JULY 2000 http://biotech.nature.com RESEARCH ARTICLES
Strategies for the in vitro Evolution of Protein Function: Enzyme Evolution by Random
Tryptophan Phosphorescence Study of Enzyme Flexibility and Unfolding in Laboratory-Evolved Thermostable Esterases
Global Incorporation of Norleucine in Place of Methionine in Cytochrome P450
Millions of years of life's struggle for survival in different environments have resulted in proteins providing
nature biotechnology VOLUME 19 MAY 2001 http://biotech.nature.com RESEARCH ARTICLE
Directed Evolution of a Cytochrome P450 Monooxygenase for Alkane Oxidation
Stability and the Evolvability of Function in a Model Protein Jesse D. Bloom,*y
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, Mar. 2005, p. 5178 Vol. 69, No. 1 1092-2172/05/$08.00 0 doi:10.1128/MMBR.69.1.5178.2005
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb. 2003, p. 987995 Vol. 69, No. 2 0099-2240/03/$08.00 0 DOI: 10.1128/AEM.69.2.987995.2003
at very negative potentials, the lock-open currents were normalized to the current level in the control case for comparison. To estimate the K1/2 of the blockers, the amount of block
Biotransformations CHIMIA 2009, 63, No. 6 309 doi:10.2533/chimia.2009.309 Chimia 63 (2009) 309312 Schweizerische Chemische Gesellschaft
Laboratory Evolution of Cytochrome P450 BM-3 Monooxygenase for
Proc. Natl. Acad. Sci. USA Vol. 95, pp. 1280912813, October 1998
ChemBioChem 2002, No. 8 WILEY-VCH-Verlag GmbH, 69451 Weinheim, Germany, 2002 1439-4227/02/03/08 $ 20.00+.50/0 781 Modification of Galactose Oxidase to
DOI: 10.1002/cbic.200500261 Direct Conversion of Ethane to
NATURE BIOTECHNOLOGY VOL 18 MARCH 2000 http://biotech.nature.com 317 The fine chemicals industry is turning increasingly to biological syn-
Engineering proteins that bind, move, make and break DNA Cynthia H Collins
19. Gaston, A. J. The ecology and behaviour of the long-tailed tit. Ibis (Lond.) 115, 330351 (1973). 20. Sharp, S. P. Kin Recognition in the Cooperative Breeding System of the Long-Tailed Tit, Aegithalos
Structure-Guided Recombination Creates an Artificial Family of Cytochromes P450
Formation of Indigo by Recombinant Mammalian Cytochrome P4501
Molecular Microbiology (2005) 55(3), 712723 doi:10.1111/j.1365-2958.2004.04437.x 2004 Blackwell Publishing Ltd
1998 Oxford University Press 681683Nucleic Acids Research, 1998, Vol. 26, No. 2 Random-priming in vitro recombination: an effective
Laboratory evolutionists continue to generate better enzymes for industrial and research applications. Exciting developments include
Research Roundup JCB VOLUME 171 NUMBER 1 200510
Thermodynamic prediction of protein neutrality Jesse D. Bloom*
A dual selection module for directed evolution of genetic YOHEI YOKOBAYASHI
A high-throughput digital imaging screen for the discovery and directed evolution of oxygenases
A structural view of evolutionary divergence Ben Spiller*, Anne Gershenson, Frances H. Arnold, and Raymond C. Stevens
Improved Product-Per-Glucose Yields in P450-Dependent Propane Biotransformations
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, June 2003, p. 35733579 Vol. 69, No. 6 0099-2240/03/$08.00 0 DOI: 10.1128/AEM.69.6.35733579.2003
This article was published as part of the C---H functionalisation in organic
Regio-and Enantioselective Alkane Hydroxylation with Engineered Cytochromes P450 BM-3
Functional Expression and Stabilization of Horseradish Peroxidase by Directed
Why High-error-rate Random Mutagenesis Libraries are Enriched in Functional and Improved Proteins
Alkene epoxidation catalyzed by cytochrome P450 BM-3 139-3 Edgardo T. Farinas,
Chemistry & Biology Diversification of Catalytic Function in a
Proc. Natl. Acad. Sci. USA Vol. 95, pp. 20352036, March 1998
nature methods | VOL.8 NO.8 | AUGUST 2011 | 623 technology feature
JOURNAL OF BACTERIOLOGY, Mar. 2004, p. 15311536 Vol. 186, No. 5 0021-9193/04/$08.00 0 DOI: 10.1128/JB.186.5.15311536.2004
Why highly expressed proteins evolve slowly D. Allan Drummond*
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 0099-2240/01/$04.00 0 DOI: 10.1128/AEM.67.9.38823887.2001
Analysis of Shuffled Gene Libraries John M. Joern, Peter Meinhold and Frances H. Arnold*
PLoS Biology | www.plosbiology.org 0663 From the bacterial colonies, each
Directed Evolution Study of Temperature Adaptation in a Psychrophilic Enzyme
NEWS AND VIEWS Breaking proteins with mutations: threads and
Structure-guided SCHEMA recombination of distantly related b-lactamases
Enantioselective r-Hydroxylation of 2-Arylacetic Acid Derivatives and Buspirone Catalyzed by Engineered Cytochrome P450 BM-3
Method to protect a targeted amino acid residue during random mutagenesis
Reduction of Dioxygen Catalyzed by Pyrene-Wired Heme Domain Cytochrome P450 BM3 Electrodes
insight review articles NATURE | VOL 409 | 11 JANUARY 2001 | www.nature.com 253
Comparison of random mutagenesis and semi-rational designed libraries for improved cytochrome P450