Raines, Ronald T. - Departments of Biochemistry & Chemistry, University of Wisconsin at Madison

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Raines, Ronald T. - Departments of Biochemistry & Chemistry, University of Wisconsin at Madison

Water-Soluble Phosphinothiols for Traceless Staudinger Ligation and Integration with Expressed Protein Ligation
Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. MECHANISTIC STUDIES, OPTIMIZATION, AND APPLICATIONS
A Ribonuclease A Variant with Low Catalytic Activity but High
Supporting Information Reciprocity of Steric and Stereoelectronic Effects in the Collagen Triple Helix
Tam, Arnold, Soellner, and Raines Supporting Information Protein Prosthesis: 1,5-Disubstituted[1,2,3]Triazoles as cis-Peptide Bond Surrogates
Disruption of Shape-Complementarity Markers to Create Cytotoxic Variants of Ribonuclease A
Tam et al. Supporting Information Water-Soluble Phosphinothiols for Traceless Staudinger Ligation
nature CHeMICaL BIOLOGY | vol 6 | august 2010 | www.nature.com/naturechemicalbiology 615 puBLIsHed OnLIne: 11 juLY 2010 | dOI: 10.1038/nCHeMBIO.406
PROTEIN ASSEMBLY TO MINE THE HUMAN GENOME BRADLEY L. NILSSON, MATTHEW B. SOELLNER
Proceedings of the 31st European Peptide Symposium
Staudinger Ligation of Peptides at Non-Glycyl Residues Matthew B. Soellner,, Annie Tam, and Ronald T. Raines*,,
Stereoelectronic and Steric Effects in the Collagen Triple Helix: Toward a Code for Strand Association
Supporting Information Tuning the pKa of Fluorescein to Optimize Binding Assays
General Acid/Base Catalysis in the Active Site of Escherichia coli Thioredoxin Peter T. Chivers and Ronald T. Raines*
Solvent Effects on the Energetics of Prolyl Peptide Bond Isomerization Supplementary Material
The Ribonucleolytic Activity of Angiogenin Peter A. Leland, Kristine E. Staniszewski, Chiwook Park,, Bradley R. Kelemen,,| and Ronald T. Raines*,,
New Concepts in Biochemistry The CXXC Motif: A Rheostat in the Active Site
AIDS RESEARCH AND HUMAN RETROVIRUSES Volume 24, Number 11, 2008
INFORMATION TO USERS The negative microfilm copy ofthis dissertation was prepared and inspected by the
Kotch, Guzei, and Raines Supporting Information Stabilization of the Collagen Triple Helix by O-Methylation of Hydroxyproline Residues
The Aberrance of the 4S Diastereomer of 4-Hydroxyproline Matthew D. Shoulders,,
Binder, Blank, and Raines Supporting Information Olefin Metathesis in
THE ROLE OF PROTEIN DISULFIDE ISOMERASE
Altering Substrate Specificity and Detecting Processivity in Nucleases
Biochemistry 1981, 20, 7509-7519 7509 Duine, J. A,, & Frank, J.(1980b) Biochem. J. 187,213-219.
Protein Expression and Purification 22, 174179 (2001) doi:10.1006/prep.2001.1422, available online at http://www.idealibrary.com on
Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. NOTE TO USERS
Supporting Information Wiley-VCH 2008
Ribonuclease S redux Rex W. Watkins, Ulrich Arnold, and Ronald T. Raines*
Stereoelectronic effects on polyproline conformation JIA-CHERNG HORNG1
Using Measurements of Anchoring Energies of Liquid Crystals on Surfaces To Quantify Proteins Captured by
ALTERING THE RESIDUES OF RIBONUCLEASE A R. T. Raines
Intraspecies Regulation of Ribonucleolytic Activity R. Jeremy Johnson, Luke D. Lavis, and Ronald T. Raines*,,
Pathway for Polyarginine Entry into Mammalian Cells Stephen M. Fuchs and Ronald T. Raines*,,
Native disulfide bond formation is critical for the proper folding of many proteins. Recent studies using newly identified protein
ALTERING THE RESIDUES OF RIBONUCLEASE A RAINES R.T.
J. Am. Chem. SOC.1992,114, 5437-5439 5437 of the surface covered by the adsorbate. Thus, plots of -In (1 -
S-Tag Rapid Assay The SeTag rapid assay is based on the
Comparative Biochemistry and Physiology Part C 123 (1999) 103111 Structural changes to ribonuclease A and their effects on
COMMUNICATION www.rsc.org/obc | Organic & Biomolecular Chemistry Electronic and steric effects on the rate of the traceless Staudinger
Reprinted from Journal of Chemical Education, Vol. 67, Page 483, June 1990. Copyright 1990 by the Division ofChemical Education of.the American Chemical Society, and reprinted by permission of
Supporting Information Selenocysteine in Native Chemical Ligation and Expressed Protein Ligation
Functional and structural analyses of N-acylsulfonamide-linked dinucleoside inhibitors of RNase A
Yatzeck et al. Supporting Information A highly sensitive fluorogenic probe
Effect of fluoro-substituted proline residues on the conformational stability of triple-helical collagen mimics
Subscriber access provided by UNIV OF WISCONSIN -MADISON Journal of the American Chemical Society is published by the American Chemical
THEJOURNALOF BIOLOGICALCHEMISTRY 0 1993by The American Society for Biochemistry and Molecular Biology, Inc
100 ." ________ - O ~~~~~~~~~
Catalysis of Protein Disulfide Bond Isomerization in a Homogeneous Substrate Elizabeth A. Kersteen,, Seth R. Barrows, and Ronald T. Raines*,,|
Hodges and Raines Supporting Information Stereoelectronic and Steric Effects in the Collagen Triple Helix
Author's personal copy Practical syntheses of 4-fluoroprolines
Soellner et al. Supporting Information Reaction Mechanism and Kinetics
Supporting Information Protein Prosthesis: A Nonnatural Residue Accelerates Folding and Increases Stability
Imaging the Binding Ability of Proteins Immobilized on Surfaces with Different Orientations by Using Liquid Crystals
Reconstitution of a Defunct Glycolytic Pathway via Recruitment of Ambiguous Sugar Kinases
Supporting Online Material n* Interactions in Proteins
INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the
Supporting Information Staudinger Ligation: A Peptide from a Thioester and Azide
Conjugate Stability DOI: 10.1002/anie.200802651
Contrast Agents for Magnetic Resonance Imaging Synthesized with Ring-Opening Metathesis Polymerization
AWARD ADDRESS 2005 Emil Thomas Kaiser Award
PROTEIN ENGINEERING OF RmONUCLEASE A RAINES R.T., RUTTER W.J.
Fluorophore Solvent max (nm) (M-1
Semisynthesis and Characterization of Mammalian Thioredoxin Reductase Brian Eckenroth, Katharine Harris, Anton A. Turanov, Vadim N. Gladyshev, Ronald T. Raines,| and
Genetic Selection for Critical Residues in Ribonucleases
Mol. Biol. Evol. 19(6):959963. 2002 2002 by the Society for Molecular Biology and Evolution. ISSN: 0737-4038
Self-assembly of synthetic collagen triple helices Frank W. Kotch and Ronald T. Raines*
Nature Macmillan Publishers Ltd 1998 Each polypeptide chain of collagen is
PROTEIN ENGINEERING TO EXPLOIT AND EXPLORE BOVINE SECRETORY RIBONUCLEASES
Supporting Information Protein Assembly by Orthogonal Chemical Ligation Methods
Tam & Raines Supporting Information Coulombic Effects on the Traceless Staudinger Ligation in Water
Macrocyclic Scaffold for the Collagen Triple Helix
Molbank 2009, M642 ISSN 1422-8599
Fluorescence Assay for the Binding of Ribonuclease A to the Ribonuclease Inhibitor Protein
SUPPORTING INFORMATION Effect of 3-Hydroxyproline Residues on Collagen Stability
Molecules 2008, 13, 204211 ISSN 1420-3049
n f * Interaction and n)( Pauli Repulsion Are Antagonistic for Protein Charles E. Jakobsche,
Catalysis by Ribonuclease A Is Limited by the Rate of Substrate Association Chiwook Park, and Ronald T. Raines*,,||
Allen et al. Supporting Information ROMP from ROMP: A New Approach to Graft Copolymer Synthesis
Hydrogen Bonds in Peptides and Proteins: Studies of Proline-Containing Peptides and Proteins
Substrate Binding and Turnover by the Highly Specific I-PpoI Endonuclease Paula K. Wittmayer and Ronald T. Raines*
ProteinProtein Interactions
Substituted 2-Azabicyclo[2.1.1]hexanes as Constrained Proline Analogues: Implications for Collagen Stability
Cytotoxic Ribonucleases: The Dichotomy of Coulombic Forces R. Jeremy Johnson, Tzu-Yuan Chao, Luke D. Lavis, and Ronald T. Raines*,,
PROTEIN EXPRESSION AND PURIFICATION 6,700-706 (1995) Production of Rat Protein Disulfide Isomerase
Biochem. J. (1995) 308, 547-550 (Printed in Great Britain) Catalytic activity of bovine seminal ribonuclease is essential for its
Supporting Information Binder et al. 10.1073/pnas.0912073107
KFERQ Sequence in Ribonuclease A-mediated Cytotoxicity* Received for publication, December 20, 2001
Activation of the Prolyl Hydroxylase Oxygen-sensor Results in Induction of GLUT1, Heme Oxygenase-1, and Nitric-oxide
Coulombic Forces in Protein-RNA Interactions: Binding and Cleavage by Ribonuclease A and Variants at Lys7, Arg10, and Lys66
A New Remote Subsite in Ribonuclease A* (Received for publication, September 28, 1998)
Walsh,Firestone, et al. 1 Mechanism-Based Enzyme Inactivation 4241 A. L.; Hruska, F. E.; Ogilvie,K. K.; Holy, A. Can. J. Chem. 1978, 56,
8606 Biochemistry 1987, 26, 8606-861 3 Nucleic Acids Res. 13, rl-r49.
Biochemistry 1989, 28, 9293-9305 9293 Evolutionary Optimization of the Catalytic Effectiveness of an Enzyme?
NOTES & TIPS 165 Peptide Tags for a Dual Affinity
Ca Copyright 1994 by the American Chemical Society Volume 33, Number 20 Accelerated Publications
CORRECTION: The "4(S)" stereochemistry herein is actually "4(R)". im. J. Peptide Protein Rn. 44. 19'N. 161-269
176 NOTES & TIPS The success of all crosslinking reagents is idiosyn-
Protein Engineering vol.8 no,3 pp,261-273, 1995 Engineering ribonuclease A: production, purification and
VOLUME117,NUMBER31 AUGUST9, 1995
J. Mol. Biol. (1995) 252, 328336 A Residue to Residue Hydrogen Bond Mediates the
The EMBO Journal vol.15 no.11 pp.2659-2667, 1996 The CXXC motif: imperatives for the formation of
rtUrientpe' effect of IOl1g
His,,,Asp Catalytic Dyad of Ribonuclease A: Structure and Function of the Wild-Type, D121N, and D121A Enzymes
Coulombic Effects of Remote Subsites on the Active Site of Ribonuclease A Barbra M. Fisher, L. Wayne Schultz, and Ronald T. Raines*
FOR THE RECORD Variants of ribonuclease inhibitor that resist oxidation
His . . . Asp Catalytic Dyad of Ribonuclease A: Histidine pKa Values in the Wild-Type, D121N, and D121A Enzymes
A small-molecule catalyst of protein folding in vitro and in vivo Kenneth J Woycechowsky1, K Dane Wittrup2* and Ronald T Raines1,3
Origin of the `inactivation' of ribonuclease A at low salt concentration Chiwook Parka
A synapomorphic disulde bond is critical for the conformational stability and cytotoxicity of an amphibian ribonuclease
Decavanadate Inhibits Catalysis by Ribonuclease A1 June M. Messmore*,2
Conformational Stability of Collagen Relies on a Stereoelectronic Effect
Contribution of Tertiary Amides to the
Selenocysteine in Native Chemical Ligation and Expressed Protein Ligation
Quantitative Analysis of the Effect of Salt Concentration on Enzymatic Catalysis
Collagen Stability: Insights from NMR Spectroscopic and Hybrid Density Functional Computational Investigations of the
Supporting Information Staudinger Ligation of -Azido Acids Retains Stereochemistry
Introduction The internalization of proteins by cells often occurs via a
Ribonuclease inhibitor as an intracellular sentry Marcia C. Haigis1
Protein Assembly by Orthogonal Chemical Ligation Methods Bradley L. Nilsson, Robert J. Hondal,, Matthew B. Soellner, and Ronald T. Raines*,,
The CXC Motif: A Functional Mimic of Protein Disulfide Isomerase Kenneth J. Woycechowsky, and Ronald T. Raines*,,|
Supplemental Material An electronic effect on protein structure
Stereoelectronic Effects on Collagen Stability: The Dichotomy of 4-Fluoroproline Diastereomers
Supporting Information Stereoelectronic Effects on Collagen Stability
Site-Specific Protein Immobilization by Staudinger Ligation Matthew B. Soellner, Kimberly A. Dickson, Bradley L. Nilsson, and Ronald T. Raines*,,
Supporting Information Site-Specific Protein Immobilization by Staudinger Ligation
Zinc(II)-mediated inhibition of ribonuclease Sa by an N-hydroxyurea nucleotide and its basis
SUPPORTING INFORMATION Substituted 2-Azabicyclo[2.1.1]hexanes
Synthetic Surfaces for Ribonuclease Adsorption Bryan D. Smith, Matthew B. Soellner, and Ronald T. Raines*,,
O-Acylation of Hydroxyproline Residues
SUPPORTING INFORMATION Cara L. Jenkins
Latent Fluorophore Based on the Trimethyl Lock Sunil S. Chandran,, Kimberly A. Dickson, and Ronald T. Raines*,,
Supporting Information Latent Fluorophore Based on the Trimethyl Lock
Jenkins et al. Supporting Information Peptide Bond Isosteres: Ester or
Cytotoxicity of Bovine Seminal Ribonuclease: Monomer versus Dimer J. Eugene Lee and Ronald T. Raines*,,
Lavis et al. Supporting Information Fluorogenic Label for Biomolecular Imaging
Reciprocity of Steric and Stereoelectronic Effects in the Collagen Triple Helix Matthew D. Shoulders, Jonathan A. Hodges,, and Ronald T. Raines*,,
Synthesis and characterization of a novel class of reducing agents that are highly neuroprotective for retinal ganglion cells
Hodges & Raines Supporting Information Energetics of an n * Interaction
Is glycine a surrogate for a D-amino acid in the collagen triple helix?
DOI: 10.1002/adsc.200600264 Salicylaldimine Ruthenium Alkylidene Complexes: Metathesis
Arginine Grafting to Endow Cell Permeability Stephen M. Fuchs,
Fuchs & Raines Supporting Information Arginine Grafting to Endow Cell-Permeability
General Method for Site-Specific Protein Immobilization by Staudinger Ligation Jeet Kalia,
Supporting Information for: Using Measurements of Anchoring Energies of Liquid Crystals on Surfaces to
Protein Prosthesis: 1,5-Disubstituted[1,2,3]triazoles as cis-Peptide Bond Annie Tam, Ulrich Arnold, Matthew B. Soellner,, and Ronald T. Raines*,,||
Genetic selection for peptide inhibitors of angiogenin Bryan D. Smith1,3 and Ronald T. Raines1,2,4
Supplementary material (ESI) for Journal of Materials Chemistry This journal is The Royal Society of Chemistry 2008
Conformational Preferences of Substrates for Human Prolyl 4-Hydroxylase Kelly L. Gorres,
Author's personal copy A highly sensitive fluorogenic probe for cytochrome P450 activity in live cells
Supporting Information Ribonuclease Inhibitor Regulates Neovascularization by Human Angiogenin
R. F. Turcotte et al. Supporting Information Onconase cytotoxicity relies on the distribution
PAPER www.rsc.org/obc | Organic & Biomolecular Chemistry Fluorogenic affinity label for the facile, rapid imaging of proteins in live cells
Stereoelectronic and steric effects in side chains preorganize a protein main chain
Supporting Text Stereoelectronic and steric effects in side chains
ISSN 1754-5692 Environmental Science
A Stereoelectronic Effect in Prebiotic Nucleotide Synthesis
Issue in Honor of Drs. Cynthia A. and Bruce E. Maryanoff ARKIVOC 2010 (viii) 251-262 Modulation of an n* interaction with -fluoro groups
Supporting Information The Aberrance of the 4S Diastereomer of 4-Hydroxyproline
Antitumor Activity of Ribonuclease Multimers Created by Site-Specific Covalent Tethering
Supporting Information Antitumor Activity of Ribonuclease Multimers
Oligomers of a 5-Carboxy-methanopyrrolidine -Amino
DOI: 10.1002/cssc.201000397 Conversion of Fructose into 5-(Hydroxymethyl)furfural in Sulfolane
SUPPLEMENTARY INFORMATION for: Tunable, posttranslational hydroxylation of collagen domains in Escherichia coli
Potentiation of ribonuclease cytotoxicity by a poly(amidoamine) dendrimer Gregory A. Ellis a
Ellis, Hornung, and Raines Supplementary Data Potentiation of ribonuclease cytotoxicity by a poly(amidoamine)
IN THIS ISSUE 1060 Crystal structure of the Leishmania major MIX protein: A scaffold
Choudhary & Raines Supporting Information CCDC Accession Number Residue No. Residue Type ! () d () " () # ()
Reprinted from Journal of Chemical Education,Yol. 65, PI!-ge 757,.September 1~88 . . Copyright 1988 by the Division of Chemical Education of the AmerIcan Chemical Society, and reprmted by permiSSIOn of
Ribonuclease A Ronald T. Raines
Cancer chemotherapy ^ ribonucleases to the rescue Peter A. Leland a
Insights on the conformational stability of collagen Cara L. Jenkins and Ronald T. Raines*
INTRODUCTION PROTEIN DISULFIDE ISOMERASE (PDI; EC 5.3.4.1) was first
Annu. Rev. Biophys. Biomol. Struct. 2005. 34:91118 doi: 10.1146/annurev.biophys.34.040204.144700
Ribonuclease Inhibitor: Structure and Function
Abstract. Facilitating the entry of molecules into mam-malian cells is of great interest to fields as diverse as
Symbiosis: Chemical Biology at Wisconsin Laura L. Kiessling*, Sally Garbo Wedde, and Ronald T. Raines
Liquid Crystal-based Analytic Technology Enabling a Molecular View of Cancer
138 Current Organic Chemistry, 2010, 14, 138-147 1385-2728/10 $55.00+.00 2010 Bentham Science Publishers Ltd.
Introduction Most polypeptides are built from 20 amino-acid building
[16) Green Fluorescent Protein Chimeras to Probe Protein-Protein Interactions
[7] Semisynthesis of Proteins Containing Selenocysteine
Jeremy R. Knowles (1935 2008) Ronald T. Raines*
Available online at www.sciencedirect.com Peptides and peptidomimetics as prototypes
Protein Chemistry DOI: 10.1002/anie.201005946
Author's personal copy Direct and continuous assay for prolyl 4-hydroxylase
Stronger and (now) Longer Synthetic Collagen Ronald T. Raines
Modulating Collagen Triple-Helix Stability with 4-Chloro, 4-Fluoro, and 4-Methylprolines
CATALYSIS IN BIOPOLYMER CHEMISTRY Joseph Bartholomew Binder
INFORMAnON TO USERS This manuscript has been reproduced from the microfilm master. UMI films
Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. RIBONUCLEASES: STRUCTURAL AND BIOCHEMICAL
INFORMAnON TO USERS The negative microfilm of this dissertation was prepared and inspected by the
Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. RIBONUCLEASE A HOMOLOGS
Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. PEPTIDE AND PROTEIN ASSEMBLY USING
Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. ENGINEERING RIBONUCLEASE-BASED
Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. RIBONUCLEASE ACTIVITY
Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. PROTEIN BACKBONES: AQUEOUS STAUDINGER LIGATIONS
Proc. Natl. Acad. Sci. USA Vol. 82, pp. 2272-2276, April 1985
7014 Biochemistry 1987, 26. 7014-7020 tralization of the positivecharge, e.g., [o-N02-Args]GnRH,
1158 Biochemistry 1988, 27, 1158-1 167 Triosephosphate Isomerase Catalysis Is Diffusion Controlled+
Protein Science (1993). 2, 348-356. Cambridge University Press. Printed in the USA. Copyright 01993The Protein Society
J. Am. Chem. SOC.1994,116,2149-2150 Amide-Amide and Amide-Water Hydrogen Bonds
J. Am. Chem. SOC.1994,116, 5467-5468 Communications to the Editor
7408 Biochemistry 1994, 33, 7408-7414 Energetics of Catalysis by Ribonucleases: Fate of the 2',3'-Cyclic Phosphodiester
174 NOTES & TIPS ~g of plasmid DNA, which is comparable to the effi-
THE JOURNAL OF BIOLOGICAL CHEMISTRY 1995 by The American Society for Biochemistry and Molecular Biology, Inc.
69SO J.Org. Chem. 1995,60,6930-6936 Mechanism-BasedInactivation of Ribonuclease A
The Essential Function of Protein-disulfide Isomerase
PROTEIN EXPRESSION AND PURIFICATION 7, 253261 (1996) ARTICLE NO. 0036
Immunobiol., 195, pp. 271-285 (1996) 1996 by Gustav Fischer Verlag, lDepartment of Physiology, Institute of Hematology and Blood Transfusion,
Protein Science (1996),5:1697-1703. Cambridge University Press. Printed in the USA. Copyright 0 1996 The Protein Society
Inductive Effects on the Energetics of Prolyl Peptide Bond Isomerization: Implications for Collagen Folding and Stability
Gene 206 (1998) 1121 Degenerate DNA recognition by I-PpoI endonuclease
Proc. Natl. Acad. Sci. USA Vol. 95, pp. 1040710412, September 1998
No Role for Pepstatin-A-Sensitive Acidic Proteinases in Reovirus Infections of L or MDCK Cells
His,,,Asp Catalytic Dyad of Ribonuclease A: Conformational Stability of the Wild-Type, D121N, D121A, and H119A Enzymes
A hyperstable collagen mimic Steven K Holmgren*, Lynn E Bretscher, Kimberly M Taylor and Ronald T Raines
Extending the Limits to Enzymatic Catalysis: Diffusion of Ribonuclease A in One Bradley R. Kelemen and Ronald T. Raines*,,
Chemical Mechanism of DNA Cleavage by the Homing Endonuclease I-PpoI Stephen J. Mannino, Cara L. Jenkins, and Ronald T. Raines*,,
Contribution of Individual Disulfide Bonds to the Oxidative Folding of Ribonuclease A
tions with hazardous organic solvents. A simple vortex adapter (like that available from Sigma) could aid in
Dimer formation by a "monomeric" protein CHIWOOK PARK1
Supporting Information High-Yielding Staudinger Ligation of a Phosphinothioester and Azide
Contribution of the Active Site Histidine Residues of Ribonuclease A to Nucleic Acid Binding
Cleavage of 3,5-Pyrophosphate-Linked Dinucleotides by Ribonuclease A and Angiogenin,
Endowing Human Pancreatic Ribonuclease with Toxicity for Cancer Cells*
Translocation of a -Peptide Across Cell Membranes Naoki Umezawa
Supporting Information Protein Prosthesis: A Semisynthetic Enzyme with a -Peptide Reverse Turn
X-ray Structure of Two Crystalline Forms of a Streptomycete Ribonuclease with Cytotoxic Activity*
An electronic effect on protein structure MATTHEW P. HINDERAKER1
Effect of 3-Hydroxyproline Residues on Collagen Stability Cara L. Jenkins, Lynn E. Bretscher,, Ilia A. Guzei,| and Ronald T. Raines*,,
Potent Inhibition of Ribonuclease A by Oligo(vinylsulfonic Acid)* Received for publication, February 21, 2003
Availableonline at www.sciencedirect.com PRESS Archives of Biochemistry and Biophysics 415 (2003) 172-177
Contribution of Active-Site Residues to the Function of Onconase, a Ribonuclease with Antitumoral Activity
Glycosylation of onconase increases its conformational stability and toxicity for cancer cells
Polyarginine as a multifunctional fusion tag STEPHEN M. FUCHS1
Peptide Bond Isosteres: Ester or (E)-Alkene in the Backbone of the
Allen, Raines, and Kiessling Supporting Information Contrast Agents for Magnetic Resonance Imaging Synthesized with Ring-
Fluorogenic Label for Biomolecular Imaging Luke D. Lavis
DOI: 10.1002/cbic.200500559 Latent Blue and Red Fluorophores
Energetics of an n f * Interaction that Impacts Protein Structure
Horng et al. Supporting Information A Macrocyclic Scaffold for the
A ribonuclease zymogen activated by the NS3 protease of the hepatitis C virus
Multilayered Films Fabricated from an Oligoarginine-Conjugated Protein Promote Efficient Surface-Mediated Protein Transduction
Genetic selection reveals the role of a buried, conserved polar residue
Tuning the pKa of Fluorescein to Optimize Binding Luke D. Lavis, Thomas J. Rutkoski, and Ronald T. Raines*,,
Author's personal copy Catalysis of imido group hydrolysis in a maleimide conjugate
Olefin Metathesis in Homogeneous Aqueous Media Catalyzed by
Increasing the potency of a cytotoxin with an arginine graft
Author's personal copy Coulombic effects on the traceless Staudinger ligation in water
Binder & Raines Supporting Information Simple Chemical Transformation of Lignocellulosic Biomass
Diazo Compounds DOI: 10.1002/anie.200804689
Supporting Information Wiley-VCH 2008
Ribonuclease Inhibitor Regulates Neovascularization by Human Angiogenin Kimberly A. Dickson,,#
Nature of Amide Carbonyl-Carbonyl Interactions in Proteins Amit Choudhary,
Supporting Information Nature of Amide CarbonylCarbonyl Interactions in Proteins
pubs.acs.org/BiochemistryPublished on Web 04/29/2009r 2009 American Chemical Society Biochemistry 2009, 48, 50515053 5051
Supporting Information Silencing an Inhibitor Unleashes a Cytotoxic Enzyme
Author's personal copy Origin of the stability conferred upon collagen by fluorination
Supporting Information Fluorogenic affinity label for the facile, rapid imaging
pubs.acs.org/Biochemistry Published on Web 08/04/2009 r 2009 American Chemical Society 8664 Biochemistry 2009, 48, 86648671
Supplementary Information S-1 5(6)-anti-Substituted-2-azabicyclo[2.1.1]hexanes. The Nucleophilic Displacement Route.
DOI: 10.1002/cssc.201000181 Synthesis of Furfural from Xylose and Xylan
Current Pharmaceutical Biotechnology, 2008, 9, 185-199 185 1389-2010/08 $55.00+.00 2008 Bentham Science Publishers Ltd.
Collagen Structure and Stability
[23J The S Tag Fusion System for Protein Purification By RONALD T. RAINES, MARK MCCORMICK, THOMAS R. VAN OOSBREE,
(5] ASSAYS FOR RIBONUCLEOLYTIC ACTIVITY [5] Fast, Facile, Hypersensitive Assays for
Provided for non-commercial research and educational use only. Not for reproduction, distribution or commercial use.
TRIOSEPHOSpJiATE ISOMERASE AND PROLINE RACEMASE: REVELATION OF REACTION ENERGETICS
Ribonuclease A: from Model System to Cancer Chemotherapeutic
THESE PROTOCOLS WERE CONTRIBUTED BY Sang-Hyun Park (Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California) and Ronald T.
ProteinProtein Interactions
Active Site of Ribonuclease A R.T. Raines
The Mechanistic Pathway of a Mutant Triosephosphate Isomerasea
Protein Assembly Using the Staudinger Ligation Bradley L. Nilsson1
Effect of 4-Fluoroproline in the X-Position on the Stability of the Collagen Triple Helix
Breaking Away: Proceedings of the 21st American Peptide Symposium
Protein Science (1998), 7:804-805. Cambridge University Press. Printed In the USA. Copyright 1998 The Protein Society
Catalysis by Ribonuclease A: Specificity, Processivity, and Mechanism
SUBSTRATE RECOGNITION BY PROLYL 4-HYDROXYLASE Kelly Lynn Gorres
Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. I. INSIGHTS INTO THE DETERMINANTS OF COLLAGEN TRIPLE HELIX
INFORMATION TO USERS The negative microfilm of this dissertation was prepared and inspected by the
INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films
A dissertation entitled Role of the HisAsp Catalytic Dyad ofRibonuclease A
CATALYSIS BY BOVINE PANCREATIC RmONUCLEASE A-ENERGETICS AND CONTRIBUTIONS FROM THE ACTIVE-SITE
RIBONUCLEASES AS CANCER AND VIRAL THERAPEUTICS: ROLE OF COULOMBIC INTERACTIONS
Biochemical Studies of the Site-Specific I-Ppol Endonuclease
INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films
Microscopic pKa Values of Escherichia coli Thioredoxin Peter T. Chivers,, Kenneth E. Prehoda,,| Brian F. Volkman,, Byung-Moon Kim, John L. Markley,, and
8232 J. Org. Chem. 2009, 74, 82328242 Published on Web 10/02/2009 DOI: 10.1021/jo901725k r 2009 American Chemical Society
Electronic Supplementary Information Synthesis and utility of fluorogenic acetoxymethyl ethers
Short Communication TheScientificWorldJOURNAL (2002) 2, 18231827
Supporting Information Copyright Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, 2011
Stringency of the 2-His1-Asp Active-Site Motif in Prolyl 4-Hydroxylase
Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. PROTEIN FOLDING ENZYMES
Inhibition of Human Pancreatic Ribonuclease by the Human Ribonuclease Inhibitor Protein
Biochemistry Supporting Information
Translocation of a -Peptide Across Cell Membranes Naoki Umezawa, Michael A. Gelman, Marcia C. Haigis, Ronald T. Raines,*,, and
Reaction Mechanism and Kinetics of the Traceless Staudinger Matthew B. Soellner,,| Bradley L. Nilsson,, and Ronald T. Raines*,,
4-Chloroprolines: Synthesis, Conformational Analysis, and Effect on the Collagen Triple Helix
Protein Prosthesis: A Semisynthetic Enzyme with a -Peptide Reverse Turn Ulrich Arnold,, Matthew P. Hinderaker, Bradley L. Nilsson, Bayard R. Huck,
Phil. Trans. R. Soc. Lond. A 317, 371~380 (1986) Printed in Great Britain
Sulfur Shuffle: Modulating Enzymatic Activity by Thiol-Disulfide Interchange
Pentavalent Organo-Vanadates as Transition State Analogues for Phosphoryl Transfer Reactions
Eur. J. Biochem. 224,109-114 (1994) FEBS 1994
ProteinScience(1998).7:1620-1625.CambridgeUniversityPress.Printed in the USA. Copyright 0 1998TheProteinSociety
Protein Prosthesis: A Nonnatural Residue Accelerates Folding and Increases Ulrich Arnold, Matthew P. Hinderaker, Jens Koditz, Ralph Golbik, Renate Ulbrich-Hofmann, and
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Mechanism of Ribonuclease Cytotoxicity* (Received for publication, August 3, 1995, and in revised form, September 27, 1995)
Binding of non-natural 3-nucleotides to ribonuclease A Cara L. Jenkins1
486 Kaminer et al. 25. K. Rupp, U. Birnbach, 1. Lundstrom P. N. Van, and H. D. Soling. Effects of
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Supplemental Material Oligomers of a 5-Carboxy-methanopyrrolidine -Amino Acid. A Search for Order.
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Supporting Information Amino acids, amino acid derivatives, and resins were from Novabiochem. Gel filtration
Stabilization of the Collagen Triple Helix by O-Methylation of Hydroxyproline Frank W. Kotch,, Ilia A. Guzei, and Ronald T. Raines*,,
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Tea* Qatar. Vol. 34. No. 19.pi. 3055-30% 1993 Printedin Grca~Britain
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Contribution of disulfide bonds to the conformational stability and catalytic activity of ribonuclease A
Catalysis of Protein Folding by an Immobilized Small-Molecule Kenneth J. Woycechowsky,, Brad A. Hook, and Ronald T. Raines*,,
Excavating an Active Site: The Nucleobase Specificity of Ribonuclease A Bradley R. Kelemen,, L. Wayne Schultz,,| Rozamond Y. Sweeney, and Ronald T. Raines*,,
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Interstrand Dipole-Dipole Interactions Can Stabilize the Collagen Triple Helix*S
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Supplemental Data Interstrand DipoleDipole Interactions Can Stabilize
Rutkoski, Kink, Strong, and Raines Supporting Information Materials. mPEGmaleimide1
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Mechanism of Ribonuclease A Endocytosis: Analogies to Cell-Penetrating Peptides
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Supporting Information A Potent, Versatile Disulfide-Reducing Agent from Aspartic Acid
Supporting Information Boronate-Mediated Biologic Delivery
A Potent, Versatile Disulfide-Reducing Agent from Aspartic Acid John C. Lukesh, III,
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