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Molecular Biology of the Cell Vol. 15, 30153030, July 2004

Summary: Molecular Biology of the Cell
Vol. 15, 3015­3030, July 2004
Cell Cycle-dependent Nuclear Localization of Yeast
RNase III Is Required for Efficient Cell DivisionV
Mathieu Catala, Bruno Lamontagne, Ste´phanie Larose, Ghada Ghazal, and
Sherif Abou Elela*
RNA Group/Groupe ARN, De´partement de Microbiologie et d'Infectiologie, Faculte´ de Me´decine,
Universite´ de Sherbrooke, Sherbrooke, Que´bec, Canada J1H 5N4
Submitted March 5, 2004; Revised April 1, 2004; Accepted April 6, 2004
Monitoring Editor: Marvin P. Wickens
Members of the double-stranded RNA-specific ribonuclease III (RNase III) family were shown to affect cell division and
chromosome segregation, presumably through an RNA interference-dependent mechanism. Here, we show that in
Saccharomyces cerevisiae, where the RNA interference machinery is not conserved, an orthologue of RNase III (Rnt1p) is
required for progression of the cell cycle and nuclear division. The deletion of Rnt1p delayed cells in both G1 and G2/M
phases of the cell cycle. Nuclear division and positioning at the bud neck were also impaired in rnt1 cells. The cell cycle
defects were restored by the expression of catalytically inactive Rnt1p, indicating that RNA cleavage is not essential for
cell cycle progression. Rnt1p was found to exit from the nucleolus to the nucleoplasm in the G2/M phase, and perturbation
of its localization pattern delayed the progression of cell division. A single mutation in the Rnt1p N-terminal domain
prevented its accumulation in the nucleoplasm and slowed exit from mitosis without any detectable effects on RNA
processing. Together, the data reveal a new role for a class II RNase III in the cell cycle and suggest that at least some


Source: Abou Elela, Sherif - Département de Microbiologie et d'Infectiologie, Université de Sherbrooke


Collections: Biology and Medicine