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Vol. 155, No. 2JOURNAL OF BACTERIOLOGY, Aug. 1983, p. 704-713 0021-9193/83/080704-10$02.00/0
 

Summary: Vol. 155, No. 2JOURNAL OF BACTERIOLOGY, Aug. 1983, p. 704-713
0021-9193/83/080704-10$02.00/0
Copyright 0 1983, American Society for Microbiology
Conditionally Replicating Plasmid Vectors That Can Integrate
into the Klebsiella pneumoniae Chromosome via
Bacteriophage P4 Site-Specific Recombination
DAVID W. OWt* AND FREDERICK M. AUSUBELt
Department ofCellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138
Received 4 April 1983/Accepted 18 May 1983
P4 is a satellite phage of P2 and is dependent on phage P2 gene products for
virion assembly and cell lysis. Previously, we showed that a virulent mutant of
phage P4 (P4 virl) could be used as a multicopy, autonomously replicating plasmid
vector in Escherichia coli and Klebsiella pneumoniae in the absence of the P2
helper. In addition to establishing lysogeny as a self-replicating plasmid, it has
been shown that P4 can also lysogenize E. coli via site-specific integration into the
host chromosome. In this study, we show that P4 also integrates into the K.
pneumoniae chromosome at a specific site. In contrast to that in E. coli, however,
site-specific integration in K. pneumoniae does not require the int gene of P4. We
utilized the alternative modes of P4 lysogenization (plasmid replication or
integration ) to construct cloning vectors derived from P4 virl that could exist in

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine