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Journal of Neuroscience Methods 144 (2005) 7989 A reusable microfluidic plate with alternate-choice architecture for

Summary: Journal of Neuroscience Methods 144 (2005) 7989
A reusable microfluidic plate with alternate-choice architecture for
assessing growth preference in tissue culture
John H. Wittig Jr.a,, Allen F. Ryanb, Peter M. Asbecka
a Department of Electrical and Computer Engineering, 9500 Gilman Drive, Mail Code 0407, University of California,
San Diego, La Jolla, CA 92093-0407, USA
b Departments of Surgery/Otolaryngology and Neurosciences, UCSD School of Medicine,
9500 Gilman Ave 0666, La Jolla, CA 92093, USA
Received 23 June 2004; received in revised form 15 October 2004; accepted 15 October 2004
We present the design of a chamber to evaluate in vitro how species and concentrations of soluble molecules control features of cell
growth--potentially including cell proliferation, cell motility, process extension, and process termination. We have created a reusable cell
culture plate that integrates a microfluidic media delivery network with standard cell culture environment. The microfluidic network delivers
a stream of cell culture media with a step-like concentration gradient down a 50100 m wide microchannel called the presentation region.
Migrating cells or growing cell processes freely choose between the two distinct chemical environments in the presentation region, but they
are forced to exclusively choose either one environment or the other when they grow past a physical barrier acting as a decision point. Our
fabrication technique requires little specialized equipment, and can be carried out in approximately 4 days per plate. We demonstrate the
effectiveness of our plates as neurites from spiral ganglion explants preferentially grow in media containing neurotrophin-3 (NT-3) as opposed
to media without NT-3. Our design could be used without modification to study dissociated cell responses to soluble growth cues, and for
behavioral screening of small motile organisms.


Source: Asbeck, Peter M. - Department of Electrical and Computer Engineering, University of California at San Diego


Collections: Engineering