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The Dam1 kinetochore complex harnesses microtubule dynamics to produce force

Summary: The Dam1 kinetochore complex harnesses
microtubule dynamics to produce force
and movement
Charles L. Asbury*
, Daniel R. Gestaut
, Andrew F. Powers*, Andrew D. Franck*, and Trisha N. Davis
Departments of *Physiology and Biophysics and Biochemistry, University of Washington, Seattle, WA 98195
Edited by James A. Spudich, Stanford University School of Medicine, Stanford, CA, and approved May 8, 2006 (received for review March 20, 2006)
Kinetochores remain attached to microtubule (MT) tips during
mitosis even as the tips assemble and disassemble under their grip,
allowing filament dynamics to produce force and move chromo-
somes. The specific proteins that mediate tip attachment are
uncertain, and the mechanism of MT-dependent force production
is unknown. Recent work suggests that the Dam1 complex, an
essential component of kinetochores in yeast, may contribute
directly to kinetochore­MT attachment and force production, per-
haps by forming a sliding ring encircling the MT. To test these
hypotheses, we developed an in vitro motility assay where beads
coated with pure recombinant Dam1 complex were bound to the
tips of individual dynamic MTs. The Dam1-coated beads remained


Source: Asbury, Chip - Department of Physiology and Biophysics, University of Washington at Seattle


Collections: Biology and Medicine