Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network

  Advanced Search  

TheJournalofExperimentalMedicine J. Exp. Med. The Rockefeller University Press 0022-1007/2003/11/1439/12 $8.00

Summary: TheJournalofExperimentalMedicine
J. Exp. Med. The Rockefeller University Press 0022-1007/2003/11/1439/12 $8.00
Volume 198, Number 9, November 3, 2003 14391450
Impaired V(D)J Recombination and Lymphocyte
Development in Core RAG1-expressing Mice
Darryll D. Dudley,1 JoAnn Sekiguchi,1 Chengming Zhu,1 Moshe J. Sadofsky,2
Scott Whitlow,1 Jeffrey DeVido,1 Robert J. Monroe,1 Craig H. Bassing,1
and Frederick W.Alt1
1Howard Hughes Medical Institute,The Children's Hospital,The Center for Blood Research,
Harvard Medical School, Boston, MA 02115
2Department of Pathology,Albert Einstein College of Medicine, Bronx, NY 10461
RAG1 and RAG2 are the lymphocyte-specific components of the V(D)J recombinase. In vitro
analyses of RAG function have relied on soluble, highly truncated "core" RAG proteins. To
identify potential functions for noncore regions and assess functionality of core RAG1 in vivo,
we generated core RAG1 knockin (RAG1c/c) mice. Significant B and T cell numbers are
generated in RAG1c/c mice, showing that core RAG1, despite missing 40% of the RAG1
sequence, retains significant in vivo function. However, lymphocyte development and the


Source: Alt,, Frederick - Immune Disease Institute, Harvard University


Collections: Biology and Medicine