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Short title: Endophytes of grasses Culturing and direct PCR suggest prevalent host generalism among diverse fungal endophytes of
 

Summary: Short title: Endophytes of grasses
Culturing and direct PCR suggest prevalent host generalism among diverse fungal endophytes of
tropical forest grasses
K. Lindsay Higgins
Phyllis D. Coley
Thomas A. Kursar
Department of Biology, University of Utah, Salt Lake City, Utah 84112
A. Elizabeth Arnold1
Division of Plant Pathology and Microbiology, School of Plant Sciences, University of Arizona,
Tucson, Arizona 85721
Abstract: Most studies examining endophytic fungi associated with grasses (Poaceae) have
focused on agronomically important species in managed ecosystems or on wild grasses in
subtropical, temperate and boreal grasslands. However grasses first arose in tropical forests,
where they remain a significant and diverse component of understory and forest-edge
communities. To provide a broader context for understanding grass-endophyte associations we
characterized fungal endophyte communities inhabiting foliage of 11 species of phylogenetically
diverse C3 grasses in the understory of a lowland tropical forest at Barro Colorado Island,
Panama. Our sample included members of early-arising subfamilies of Poaceae that are endemic
to forests, as well as more recently arising subfamilies that transitioned to open environments.
Isolation on culture media and direct PCR and cloning revealed that these grasses harbor species-

  

Source: Arnold, A. Elizabeth - School of Plant Sciences, University of Arizona

 

Collections: Biology and Medicine