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Vol. 170, No. 12 Interference between Rhizobium meliloti and Rhizobium trifolii
 

Summary: Vol. 170, No. 12
Interference between Rhizobium meliloti and Rhizobium trifolii
Nodulation Genes: Genetic Basis of R. meliloti Dominance
FREDERIC DEBELLEt,2 FABIENNE MAILLET,2 JACQUES VASSE,2 CHARLES ROSENBERG,2 FRANCOISE
DE BILLY,2 GEORGES TRUCHET,2 JEAN DENARIE,2 AND FREDERICK M. AUSUBEL1*
Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General
Hospital, Boston, Massachusetts 02114,' and Laboratoire de Biologie Mole'culaire des Relations Plantes-
Microorganismes, Centre National de la Recherche Scientifique-Institut National de la Recherche Agronomique, 31326
Castanet Tolosan Cedex, France2
Received 16 May 1988/Accepted 9 September 1988
Transfer of an IncP plasmid carrying the Rhizobium meliloti nodFE, nodG, and nodH genes to Rhizobium
trifolii enabled R. trifolii to nodulate alfalfa (Medicago sativa), the normal host ofR. meliloti. Using transposon
Tn5-linked mutations and in vitro-constructed deletions of the R. meliloti nodFE, nodG, and nodH genes, we
showed that R. meliloti nodH was required for R. trifolii to elicit both root hair curling and nodule initiation
on alfalfa and that nodH, nodFE, and nodG were required for R. trifolii to elicit infection threads in alfalfa root
hairs. Interestingly, the transfer of the R. meliloti nodFE, nodG, and nodH genes to R. trifolii prevented R.
trifolii from infecting and nodulating its normal host, white clover (Trifolium repens). Experiments with the
mutated R. meliloti nodH, nodF, nodE, and nodG genes demonstrated that nodH, nodF, nodE, and possibly
nodG have an additive effect in blocking infection and nodulation of clover.
Bacteria of the genus Rhizobium elicit nitrogen-fixing

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine