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Accelerated Articles An Integrated Microfabricated Cell Sorter
 

Summary: Accelerated Articles
An Integrated Microfabricated Cell Sorter
Anne Y. Fu,, Hou-Pu Chou,, Charles Spence, Frances H. Arnold, and Stephen R. Quake*,
Department of Applied Physics, California Institute of Technology, MC 128-95, Pasadena, California 91125, and Division of
Chemistry and Chemical Engineering, California Institute of Technology, MC 210-41, Pasadena, California 91125
We have developed an integrated microfabricated cell
sorter using multilayer soft lithography. This integrated
cell sorter is incorporated with various microfluidic func-
tionalities, including peristaltic pumps, dampers, switch
valves, and input and output wells, to perform cell sorting
in a coordinated and automated fashion. The active
volume of an actuated valve on this integrated cell sorter
can be as small as 1 pL, and the volume of optical
interrogation is 100 fL. Different algorithms of cell
manipulation, including cell trapping, were implemented
in these devices. We have also demonstrated sorting and
recovery of Escherichia coli cells on the chip.
Since the introduction of the first microfabricated electrokinetic
analytical device in 1991,1 much work has been focused on using
electrokinetic forces to separate ionic species such as peptides

  

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology
Quake, Stephen R. - Department of Bioengineering, Stanford University

 

Collections: Biology and Medicine; Biotechnology; Chemistry; Engineering