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Association of hydrophobically-modified poly(ethylene glycol) with fusogenic liposomes

Summary: Association of hydrophobically-modified poly(ethylene glycol) with
fusogenic liposomes
Debra T. Augustea
, Robert K. Prud'hommea,*, Patrick L. Ahlb,1
, Paul Meersb,2
, Joachim Kohnc
Department of Chemical Engineering, Princeton University, Engineering Quadrangle, Princeton, NJ 08544, USA
Drug Delivery, Elan Pharmaceuticals, One Research Way, Princeton, NJ 08540, USA
Department of Chemistry, Rutgers University, 610 Taylor Road, Piscataway, NJ 08854, USA
Received 20 March 2003; received in revised form 12 August 2003; accepted 28 August 2003
We present results on using cooperative interactions to shield liposomes by incorporating multiple hydrophobic anchoring sites on
polyethylene glycol (PEG) polymers. The hydrophobically-modified PEGs (HMPEGs) are comb-graft polymers with strictly alternating
monodisperse PEG blocks (Mw=6, 12, or 35 kDa) bonded to C18 stearylamide hydrophobes. Cooperativity is varied by changing the degree
of oligomerization at a constant ratio of PEG to stearylamide. Fusogenic liposomes prepared from N-C12-DOPE:DOPC 7:3 (mol:mol) were
equilibrated with HMPEGs. Affinity for polymer association to liposomes increases with the degree of oligomerization; equilibrium constants
(given as surface coverage per equilibrium concentration of free polymer) for 6 kDa PEG increased from 6.1F0.8 (mg/m2


Source: Auguste, Debra T. - School of Engineering and Applied Sciences, Harvard University


Collections: Materials Science; Biology and Medicine