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Association of hydrophobically-modified poly(ethylene glycol) with fusogenic liposomes
 

Summary: Association of hydrophobically-modified poly(ethylene glycol) with
fusogenic liposomes
Debra T. Augustea
, Robert K. Prud'hommea,*, Patrick L. Ahlb,1
, Paul Meersb,2
, Joachim Kohnc
a
Department of Chemical Engineering, Princeton University, Engineering Quadrangle, Princeton, NJ 08544, USA
b
Drug Delivery, Elan Pharmaceuticals, One Research Way, Princeton, NJ 08540, USA
c
Department of Chemistry, Rutgers University, 610 Taylor Road, Piscataway, NJ 08854, USA
Received 20 March 2003; received in revised form 12 August 2003; accepted 28 August 2003
Abstract
We present results on using cooperative interactions to shield liposomes by incorporating multiple hydrophobic anchoring sites on
polyethylene glycol (PEG) polymers. The hydrophobically-modified PEGs (HMPEGs) are comb-graft polymers with strictly alternating
monodisperse PEG blocks (Mw=6, 12, or 35 kDa) bonded to C18 stearylamide hydrophobes. Cooperativity is varied by changing the degree
of oligomerization at a constant ratio of PEG to stearylamide. Fusogenic liposomes prepared from N-C12-DOPE:DOPC 7:3 (mol:mol) were
equilibrated with HMPEGs. Affinity for polymer association to liposomes increases with the degree of oligomerization; equilibrium constants
(given as surface coverage per equilibrium concentration of free polymer) for 6 kDa PEG increased from 6.1F0.8 (mg/m2

  

Source: Auguste, Debra T. - School of Engineering and Applied Sciences, Harvard University

 

Collections: Materials Science; Biology and Medicine