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0014-2980/00/0404-1127$17.50+.50/0 WILEY-VCH Verlag GmbH, D-69451 Weinheim, 2000 Antigen-presenting cells recruited by Brugia malayi
 

Summary: 0014-2980/00/0404-1127$17.50+.50/0© WILEY-VCH Verlag GmbH, D-69451 Weinheim, 2000
Antigen-presenting cells recruited by Brugia malayi
induce Th2 differentiation of našive CD4+
T cells
P'ng Loke, Andrew S. MacDonald and Judith E. Allen
Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh, GB
A key feature of nematode infection is a bias towards a type 2 immune response. To investi-
gate the role that antigen-presenting cells (APC) may play in promoting this bias, we used
adherent peritoneal exudate cells (PEC) recruited in response to the filarial nematode Brugia
malayi, to stimulate našive T cells from pigeon cytochrome c (PCC)-specific TCR transgenic
(PCC-tg) mice. Although the proliferation of PCC-tg T cells was inhibited by parasite-
induced PEC during primary stimulation, they proliferated normally upon secondary stimula-
tion and were not rendered anergic. However, PCC-tg T cells primed by suppressive APC
differentiated into IL-4-producing Th2 cells upon secondary stimulation instead of IFN- + -
producing Th1 cells, as has been previously described. Studies with carboxyfluorescein dia-
cetate succinimidyl ester (CFSE)-labeled cells indicated that Th2 differentiation was associ-
ated with the inhibition of (or failure to stimulate) IFN- + production during primary stimula-
tion. Interestingly, blocking antibodies against TGF- g (but not IL-10) restored the differentia-
tion of IFN- + -producing Th1 cells. Identical results with CFSE-labeled cells were obtained
using purified IL-4-dependent F4/80+

  

Source: Allen, Judith - School of Biological Sciences, University of Edinburgh

 

Collections: Biology and Medicine