Summary: © 2004The Royal Microscopical Society
Journal of Microscopy,Vol. 216, Pt 1 October 2004, pp. 3248
Received 25 January 2004; accepted 26 May 2004
Blackwell Publishing, Ltd.
Phase-retrieved pupil functions in wide-field fluorescence
B. M. HANSER*, M. G. L. GUSTAFSSON§, D. A. AGARD &
J. W. SEDAT
*Graduate Group in Biophysics, and Department of Biochemistry and Biophysics, University of
California San Francisco, San Francisco, CA 94143-2240, U.S.A.
Key words. Deconvolution, fluorescence microscopy, phase retrieval, PSF,
three-dimensional (3D) imaging properties of wide-field opti-
cal systems.The pupil function of a microscope can be compu-
tationally estimated from the measured point spread function
(PSF) using phase retrieval algorithms. The compaction of a
3D PSF into a 2D pupil function suppresses artefacts and