| | |
Summary: Transcription factor network reconstruction using the living cell array
Eric Yang, Martin L. Yarmush, Ioannis P. Androulakis Ã
Department of Biomedical Engineering, Rutgers University, 599 Taylor Road, Piscataway, NJ 08854, USA
a r t i c l e i n f o
Article history:
Received 10 May 2008
Received in revised form
28 August 2008
Accepted 26 September 2008
Available online 22 October 2008
Keywords:
Systems biology
Network reconstruction
a b s t r a c t
The objective of identifying transcriptional regulatory networks is to provide insights as to what governs
an organism's long term response to external stimuli. We explore the coupling of the living cell array
(LCA), a novel microfluidics device which utilizes fluorescence levels as a surrogate for transcription
factor activity with reverse Euler deconvolution (RED) a computational technique proposed in this work
to decipher the dynamics of the interactions. It is hypothesized that these two methods will allow us to
first assess the underlying network architecture associated with the transcription factor network as well
|
