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MOLECULAR AND CELLULAR BIOLOGY, Dec. 1981, p. 1125-1137 Vol. 1, No. 12 0270-7306/81/121 125-13$02.00/0
 

Summary: MOLECULAR AND CELLULAR BIOLOGY, Dec. 1981, p. 1125-1137 Vol. 1, No. 12
0270-7306/81/121 125-13$02.00/0
Sequence Heterogeneity, Multiplicity, and Genomic
Organization of a- and f3-Tubulin Genes in Sea Urchins
DESPINA ALEXANDRAKI* AND JOAN V. RUDERMAN
Department ofAnatomy and Department of Cell and Developmental Biology, Harvard Medical School,
Boston, Massachusetts 02115
Received 8 May 1981/Accepted 12 August 1981
We analyzed the multiplicity, heterogeneity, and organization of the genes
encoding the a and ,B tubulins in the sea urchin Lytechinuspictus by using cloned
complementary deoxyribonucleic acid (cDNA) and genomic tubulin sequences.
cDNA clones were constructed by using immature spermatogenic testis polyad-
enylic acid-containing ribonucleic acid as a template. a- and ,8-tubulin clones were
identified by hybrid selection and in vitro translation of the corresponding
messenger ribonucleic acids, followed by immunoprecipitation and two-dimen-
sional gel electrophoresis of the translation products. The a cDNA clone contains
a sequence that encodes the 48 C-terminal amino acids of a tubulin and 104 base
pairs of the 3' nontranslated portion of the messenger ribonucleic acid. The ,B
cDNA insertion contains the coding sequence for the 100 C-terminal amino acids
of f8 tubulin and 83 base pairs of the 3' noncoding sequence. Hybrid selections

  

Source: Alexandraki, Despina - Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas

 

Collections: Biology and Medicine; Biotechnology