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Charles L. Asbury1 Alan H. Diercks2

Summary: Charles L. Asbury1
Alan H. Diercks2
Ger van den Engh2
Biological Sciences,
Stanford University,
Stanford, CA, USA
Institute for Systems Biology,
Seattle, WA, USA
Trapping of DNA by dielectrophoresis
Under suitable conditions, a DNA molecule in solution will develop a strong electric
dipole moment. This induced dipole allows the molecule to be manipulated with field
gradients, in a phenomenon known as dielectrophoresis (DEP). Pure dielectrophoretic
motion of DNA requires alternate current (AC) electric fields to suppress the electro-
phoretic effect of the molecules net charge. In this paper, we present two methods for
measuring the efficiency of DEP for trapping DNA molecules as well as a set of quanti-
tative measurements of the effects of strand length, buffer composition, and frequency
of the applied electric field. A simple configuration of electrodes in combination with a
microfluidic flow chamber is shown to increase the concentration of DNA in solution by


Source: Asbury, Chip - Department of Physiology and Biophysics, University of Washington at Seattle


Collections: Biology and Medicine