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Charles L. Asbury1 Alan H. Diercks2
 

Summary: Charles L. Asbury1
Alan H. Diercks2
Ger van den Engh2
1
Biological Sciences,
Stanford University,
Stanford, CA, USA
2
Institute for Systems Biology,
Seattle, WA, USA
Trapping of DNA by dielectrophoresis
Under suitable conditions, a DNA molecule in solution will develop a strong electric
dipole moment. This induced dipole allows the molecule to be manipulated with field
gradients, in a phenomenon known as dielectrophoresis (DEP). Pure dielectrophoretic
motion of DNA requires alternate current (AC) electric fields to suppress the electro-
phoretic effect of the molecules net charge. In this paper, we present two methods for
measuring the efficiency of DEP for trapping DNA molecules as well as a set of quanti-
tative measurements of the effects of strand length, buffer composition, and frequency
of the applied electric field. A simple configuration of electrodes in combination with a
microfluidic flow chamber is shown to increase the concentration of DNA in solution by

  

Source: Asbury, Chip - Department of Physiology and Biophysics, University of Washington at Seattle

 

Collections: Biology and Medicine