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Summary: SOP FOR ASBURY LAB (G212, HSB) University of Washington
Standard Operating Procedures for Chemicals or Processes
#1 Process
Coverslip Cleaning for TIRF and TRAP Assays
Used in coverslip cleaning.
#2 Chemicals Involved
Ethanol, Potassium
Hydroxide (KOH),
Hydrogen Peroxide
and Sulfuric Acid
Flammable solvents &
Acids/Bases
Flammable solvent vapors can travel and can produce fire and explosion if an ignition
source is contacted. Some flammable solvents are more hazardous than others. Many
solvents also have an effect on the central nervous system and at high concentrations
cause sedation, coma and death. Contact with solvents can de-lipify skin and cause
irritation of skin and mucous membranes.
The Hydrogen Peroxide + Sulfuric Acid mixture (Piranha Solution) should be handled
with extreme caution. Follow all procedures involving personal protection outlined
below. *NEVER* handle this solution outside of the fume hood (see location below).
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