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Intronic Sequences Flanking Alternatively Spliced Exons Are Conserved Between Human and Mouse

Summary: Intronic Sequences Flanking Alternatively Spliced
Exons Are Conserved Between Human and Mouse
Rotem Sorek1,2
and Gil Ast1,3
Department of Human Genetics, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel; 2
Ltd., Tel Aviv 69512, Israel
Comparison of the sequences of mouse and human genomes revealed a surprising number of nonexonic,
nonexpressed conserved sequences, for which no function could be assigned. To study the possible correlation
between these conserved intronic sequences and alternative splicing regulation, we developed a method to
identify exons that are alternatively spliced in both human and mouse. We compiled two exon sets: one of
alternatively spliced conserved exons and another of constitutively spliced conserved exons. We found that 77%
of the conserved alternatively spliced exons were flanked on both sides by long conserved intronic sequences. In
comparison, only 17% of the conserved constitutively spliced exons were flanked by such conserved intronic
sequences. The average length of the conserved intronic sequences was 103 bases in the upstream intron and 94
bases in the downstream intron. The average identity levels in the immediately flanking intronic sequences were
88% and 80% for the upstream and downstream introns, respectively, higher than the conservation levels of
77% that were measured in promoter regions. Our results suggest that the function of many of the intronic
sequence blocks that are conserved between human and mouse is the regulation of alternative splicing.


Source: Ast, Gil - Department of Molecular Genetics and Biochemistry, Tel Aviv University
Sorek, Rotem - Department of Molecular Genetics, Weizmann Institute of Science


Collections: Biology and Medicine