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Immunofluorescence method by using paraformaldehyde and glutaraldehyde
 

Summary: 1
Immunofluorescence method by using paraformaldehyde and
glutaraldehyde
Aim : To observe, in situ, yeast's proteins with tagged antibodies.
Material :
- PPB dibasic, 1 M, pH 9.3
- Paraformaldehyde buffer 10% (PFB 10%)
- H3PO4 conc.
- 50% Glutaraldehyde
- 2 x big centrifugation tubes (500 ml)
- PPB 6.5
- PBS 1X
- Buffer A
- Buffer B
- Multiwell slide
- Polylysine stock solution
- -mercaptoéthanol
- Zymolase solution (20 mg/ml)
Solutions :
1. Potassium phosphate buffer dibasic (PPB) 1 M, pH 9.3

  

Source: Abou Elela, Sherif - Département de Microbiologie et d'Infectiologie, Université de Sherbrooke

 

Collections: Biology and Medicine