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Original Contribution NUCLEAR FACTOR-B MEDIATES OVER-EXPRESSION OF
 

Summary: Original Contribution
NUCLEAR FACTOR- B MEDIATES OVER-EXPRESSION OF
CYCLOOXYGENASE-2 DURING ACTIVATION OF RAW 264.7
MACROPHAGES IN SELENIUM DEFICIENCY
FAITH ZAMAMIRI-DAVIS, YING LU, JERRY T. THOMPSON, K. SANDEEP PRABHU, PADALA V. REDDY,
LORRAINE M. SORDILLO, and C. CHANNA REDDY
Department of Veterinary Science and Center of Molecular Toxicology and Carcinogenesis, The Pennsylvania State University,
University Park, PA, USA
(Received 12 December 2001; Accepted 29 January 2002)
Abstract--Selenium (Se) is an essential micronutrient for all mammalian species and is associated with a variety of
physiological functions, notably immune system, in the form of selenoproteins. Inadequate Se nutrition has been linked
to various diseases, including rheumatoid arthritis, cardiomyopathy, and cancer. Important to this discussion is that
cyclooxygenase-2 (COX-2) is over-expressed in all the aforesaid pathologies; however, a casual relationship between
Se status and COX-2 expression remains to be established. The present study is based on the hypothesis that oxidant
stress, a consequence of Se deficiency, lowers the activation potential of the redox-sensitive transcription factor, NF- B,
and that the activated NF- B is required for the altered expression of COX-2. To test this hypothesis, we have
investigated the relationship between Se status and COX-2 expression in response to LPS stimulation in RAW 264.7,
a macrophage-like cell line. In Se-deficient cells, the Se-dependent glutathione peroxidase activity (Se-GPx), a measure
of Se status, was markedly reduced and the overall oxidative stress was significantly higher than Se-supplemented cells.
Upon lipopolysaccharide (LPS) stimulation, we found 2-3-folds higher COX-2 protein expression as well as higher

  

Source: Andrews, Anne M. - Huck Institutes of the Life Sciences, Pennsylvania State University

 

Collections: Biology and Medicine