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RESEARCH ARTICLE Open Access Antibody isotype analysis of malaria-nematode
 

Summary: RESEARCH ARTICLE Open Access
Antibody isotype analysis of malaria-nematode
co-infection: problems and solutions associated
with cross-reactivity
Karen J Fairlie-Clarke1*
, Tracey J Lamb2
, Jean Langhorne3
, Andrea L Graham1,4
, Judith E Allen1
Abstract
Background: Antibody isotype responses can be useful as indicators of immune bias during infection. In studies of
parasite co-infection however, interpretation of immune bias is complicated by the occurrence of cross-reactive
antibodies. To confidently attribute shifts in immune bias to the presence of a co-infecting parasite, we suggest
practical approaches to account for antibody cross-reactivity. The potential for cross-reactive antibodies to influence
disease outcome is also discussed.
Results: Utilising two murine models of malaria-helminth co-infection we analysed antibody responses of mice
singly- or co-infected with Plasmodium chabaudi chabaudi and Nippostrongylus brasiliensis or Litomosoides
sigmodontis. We observed cross-reactive antibody responses that recognised antigens from both pathogens
irrespective of whether crude parasite antigen preparations or purified recombinant proteins were used in ELISA.
These responses were not apparent in control mice. The relative strength of cross-reactive versus antigen-specific

  

Source: Allen, Judith - School of Biological Sciences, University of Edinburgh
Graham, Andrea L. - School of Biological Sciences, University of Edinburgh

 

Collections: Biology and Medicine