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Complex expression of natural killer receptor genes in single natural killer cells ZAHEED HUSAIN,*{{ CHESTER A. ALPER,{1 EDMOND J. YUNIS*{ &
 

Summary: Complex expression of natural killer receptor genes in single natural killer cells
ZAHEED HUSAIN,*{{ CHESTER A. ALPER,{1 EDMOND J. YUNIS*{ &
DEVENDRA P. DUBEY{ *Dana-Farber Cancer Institute, {The Center for Blood Research, and the
Departments of {Pathology and 1Pediatrics, Harvard Medical School, Boston, MA, USA
SUMMARY
Human natural killer (NK) cells express several inhibitory and non-inhibitory NK receptors
per cell. Understanding the expression patterns of these receptor genes in individual cells
is important to understanding their function. Using a single-cell reverse transcription­
polymerase chain reaction (RT-PCR) method, we analysed the expression of nine NK
receptor genes in 38 resting CD56+
NK cells from peripheral blood of normal donors. We
observed highly diverse patterns of receptor expression in these cells. No NK receptor is
expressed universally in every CD56+
NK cell. The expressed receptor types per cell varied
from two to eight. We specifically analysed the distribution of inhibitory (DL) and non-
inhibitory (DS) killer immunoglobulin-like receptors (KIR). The frequency of individual
receptor expression varied from 26% for 2DS2 to 68% for both 2DL1 and 2DL4. A
comparison of the coexpression of DL and DS receptors showed a significant association in
the expression of 2DL2 and 2DS2 (x2
=16.6; P<0.001) genes but no association between

  

Source: Alper, Chester A. - Immune Disease Institute, Harvard University

 

Collections: Biology and Medicine