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Supplementary Figure 1. High fat diet induced obesity increased islet Cck expression. BTBR mice1 were placed on 60% saturated fat (HFD) or semi-purified (control) diet for 33 weeks at the time of2
 

Summary:  1
Supplementary Figure 1. High fat diet induced obesity increased islet Cck expression. BTBR mice1
were placed on 60% saturated fat (HFD) or semi-purified (control) diet for 33 weeks at the time of2
weaning. Body weight (A) and fasting plasma insulin (B) levels increased with diet-induced obesity3
(N=5). (C) Cck mRNA abundance was measured by quantitative RT-PCR with Taqman probes and4
normalized to -actin levels (N=3). Plasma insulin levels were log10 transformed. All comparisons were5
made by Student's unpaired t-tests.6
Supplementary Figure 2. CCK is expressed in - and -cells of obese mice. Immunofluorescence7
imaging of insulin (red) and glucagon (green) in a representative islet from two CcklacZ/+
-ob/ob mice (A,8
C). X-gal staining (blue) was subsequently performed and imaged on identical sections and overlayed9
with immunofluorescence images (B, D). White arrows indicate X-gal positive -cells and yellow arrows10
indicated X-gal positive -cells.11
Supplementary Figure 3. Loss of CCK does not affect glucose tolerance, insulin secretion, or insulin12
sensitivity. Glucose (A) and insulin (B) curves on overnight fasted 10 week old male CckWT
-ob/ob (N=9)13
and CcklacZ
-ob/ob mice (N=10) after IP-GTT. (C) Glucose-stimulated insulin secretion of statically14
incubated 14 week old female CckWT
-ob/ob (N=4) and CcklacZ

  

Source: Attie, Alan D. - Department of Biochemistry, University of Wisconsin at Madison

 

Collections: Biology and Medicine