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Genome-Wide RNAi of C. elegans Using the Hypersensitive rrf-3 Strain

Summary: Genome-Wide RNAi of C. elegans
Using the Hypersensitive rrf-3 Strain
Reveals Novel Gene Functions
Femke Simmer1[
, Celine Moorman1[
, Alexander M. van der Linden1[
, Ewart Kuijk1
, Peter V. E. van den Berghe1
Ravi S. Kamath2
, Andrew G. Fraser2
, Julie Ahringer2
, Ronald H. A. Plasterk1*
1 Hubrecht Laboratory, Centre for Biomedical Genetics, Utrecht, The Netherlands, 2 University of Cambridge, Wellcome Trust/Cancer Research Institute and Department of
Genetics, Cambridge, United Kingdom
RNA-mediated interference (RNAi) is a method to inhibit gene function by introduction of double-stranded RNA
(dsRNA). Recently, an RNAi library was constructed that consists of bacterial clones expressing dsRNA, corresponding
to nearly 90% of the 19,427 predicted genes of C. elegans. Feeding of this RNAi library to the standard wild-type
laboratory strain Bristol N2 detected phenotypes for approximately 10% of the corresponding genes. To increase the
number of genes for which a loss-of-function phenotype can be detected, we undertook a genome-wide RNAi screen


Source: Ahringe, Julie - Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge


Collections: Biology and Medicine