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Rab11a-dependent exocytosis of discoidal/fusiform vesicles in bladder umbrella cells

Summary: Rab11a-dependent exocytosis of discoidal/fusiform
vesicles in bladder umbrella cells
Puneet Khandelwal*, Wily G. Ruiz*, Elena Balestreire-Hawryluk*
, Ora A. Weisz*
, James R. Goldenring§
and Gerard Apodaca*¶
*Laboratory of Epithelial Cell Biology and Renal Electrolyte Division of the Department of Medicine, and Department of Cell Biology and Physiology,
University of Pittsburgh, Pittsburgh, PA 15261; Departments of Surgery and Cell and Developmental Biology, Vanderbilt University School of Medicine,
and §Nashville Veterans Administration Medical Center, Nashville, TN 37232
Edited by David D. Sabatini, New York University School of Medicine, New York, NY, and approved August 29, 2008 (received for review June 10, 2008)
The discoidal/fusiform vesicles (DFV) of bladder umbrella cells
undergo regulated exocytosis in response to stretch, but little is
known about their biogenesis or the molecular machinery that
modulates this process. We observed that Rab11a was expressed
in umbrella cells (but not Rab11b or Rab25) and was associated
with DFV. Using adenovirus-mediated delivery we transduced
umbrella cells in situ with either dominant active (DA) or dominant
negative (DN) mutants of Rab11a. DA-Rab11a stimulated an in-
crease in apical surface area in the absence of stretch, whereas


Source: Apodaca, Gerard - Departments of Medicine & Cell Biology and Physiology, University of Pittsburgh
Weisz, Ora A. - Laboratory of Epithelian Cell Biology, University of Pittsburgh


Collections: Biology and Medicine