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Interferon q suppresses glucocorticoid augmentation of macrophage clearance of

Summary: Interferon q suppresses glucocorticoid
augmentation of macrophage clearance of
apoptotic cells
Sarah J. Heasman, Katherine M. Giles, Adriano G. Rossi, Judith E. Allen, Christopher
Haslett and Ian Dransfield
MRC Centre for Inflammation Research, University of Edinburgh Medical School, Edinburgh, GB
One of beneficial effects of glucocorticoids (GC) in inflammation may be the augmentation of
macrophages' capacity for phagocytosis of apoptotic cells, a process that has a central role
in resolution of inflammation. Here we define the phenotype of GC-treated monocyte-
derived macrophages, comparing to IFN- + -treated and IL-4-treated monocyte-derived mac-
rophages and combinatorial treatment. Our data indicate that the cytokine microenviron-
ment at an inflammatory site will critically determine monocyte functional capacity following
treatment with GC. In particular, whilst GC exert dominant regulatory effects over IFN- + in
terms of cell surface receptor repertoire and morphology, the acquisition of a macrophage
capacity for clearance of apoptotic cells is prevented by combined treatment. In terms of
mechanism, GC augmentation of phagocytosis was reversed even when monocytes were
pre-incubated with GC for the first 24 h of culture, a period that is critical for induction of a
highly phagocytic macrophage phenotype. These findings have important implications for
the effectiveness of GC in promoting acquisition of a pro-phagocytic macrophage pheno-
type in inflammatory diseases associated with high levels of IFN- +


Source: Allen, Judith - School of Biological Sciences, University of Edinburgh


Collections: Biology and Medicine