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APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2009, p. 49134918 Vol. 75, No. 14 0099-2240/09/$08.00 0 doi:10.1128/AEM.00246-09
 

Summary: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 2009, p. 49134918 Vol. 75, No. 14
0099-2240/09/$08.00 0 doi:10.1128/AEM.00246-09
Copyright 2009, American Society for Microbiology. All Rights Reserved.
Quantitative Real-Time PCR Assay for QPX (Thraustochytriidae), a
Parasite of the Hard Clam (Mercenaria mercenaria)
Qianqian Liu, Bassem Allam, and Jackie L. Collier*
School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, New York 11794-5000
Received 1 February 2009/Accepted 15 May 2009
We developed a real-time quantitative PCR (qPCR) assay targeting the rRNA internal transcribed spacer
region of the hard clam pathogen QPX. The qPCR assay was more sensitive than was histology in detecting
clams with light QPX infections. QPX was detected in 4 of 43 sediment samples but in none of 40 seawater
samples.
The thraustochytrid called QPX (for quahog parasite un-
known) has caused high mortalities in hatchery-reared and wild
hard clams (Mercenaria mercenaria, also known as quahogs) from
Prince Edward Island (Canada) to Virginia (United States) since
the late 1950s (17, 22, 25, 29). In the summer of 2002, QPX
infections appeared in the previously healthy Raritan Bay (off
the coast of Staten Island in New York) M. mercenaria popu-
lation, causing significant clam mortality and closure of the

  

Source: Allam, Bassem - School of Marine and Atmospheric Sciences, SUNY at Stony Brook

 

Collections: Biology and Medicine; Environmental Sciences and Ecology