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SNAP-23 Requirement for Transferrin Recycling in Streptolysin-O-permeabilized Madin-Darby Canine Kidney Cells*

Summary: SNAP-23 Requirement for Transferrin Recycling in Streptolysin-
O-permeabilized Madin-Darby Canine Kidney Cells*
(Received for publication, February 2, 1998, and in revised form, April 27, 1998)
Som-Ming Leung, Dong Chen§, Bibhuti R. DasGupta¶, Sidney W. Whiteheart§,
and Gerard Apodaca
From the Laboratory of Epithelial Cell Biology, the Renal-Electrolyte Division of the Department of Medicine and
Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, the §Department
of Biochemistry, University of Kentucky, Lexington, Kentucky 40536, and the ¶Department of Food Microbiology and
Toxicology, University of Wisconsin, Madison, Wisconsin 53706
Fusion of recycling and transcytotic vesicles with the
apical and basolateral plasma membrane domains of
Madin-Darby canine kidney (MDCK) cells requires the
N-ethylmaleimide-sensitive factor and is sensitive to
botulinum neurotoxin serotype E (BoNT/E). BoNT/E is
thought to selectively proteolyze the 25,000-dalton syn-
aptosomal associated protein (SNAP-25), a protein
found in neurons or cells of neuroendocrine origin.
However, SNAP-25 is not found in MDCK cells. One pos-
sible target for BoNT/E in MDCK cells is SNAP-23, a
newly described SNAP-25 homolog that is found in sev-


Source: Apodaca, Gerard - Departments of Medicine & Cell Biology and Physiology, University of Pittsburgh


Collections: Biology and Medicine