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Theor Appl Genet (2003) 107:12011207 DOI 10.1007/s00122-003-1370-5

Summary: Theor Appl Genet (2003) 107:12011207
DOI 10.1007/s00122-003-1370-5
M. V. Ashley J. A. Wilk S. M. N. Styan K. J. Craft
K. L. Jones K. A. Feldheim K. S. Lewers
T. L. Ashman
High variability and disomic segregation of microsatellites
in the octoploid Fragaria virginiana Mill. (Rosaceae)
Received: 10 August 2002 / Accepted: 11 June 2003 / Published online: 8 August 2003
Springer-Verlag 2003
Abstract The objectives of the present study were to
develop microsatellite markers for the wild strawberry,
Fragaria virginiana, to evaluate segregation patterns of
microsatellite alleles in this octoploid species, and assess
genetic variability at microsatellite loci in a wild popu-
lation. A genomic library was screened for microsatellite
repeats and several PCR primers were designed and
tested. We also tested the use of heterologous primers and
found that F. virginiana primers amplified products in
cultivated strawberry, Fragaria ananassa Duch. and
Fragaria chiloensis. Similarly, microsatellite loci devel-


Source: Ashley, Mary V. - Department of Biological Sciences, University of Illinois at Chicago


Collections: Biology and Medicine; Environmental Sciences and Ecology