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Design of Solenoidal Microcoils for High-Resolution C NMR Spectroscopy

Summary: Design of Solenoidal Microcoils for High-Resolution
C NMR Spectroscopy
Raju Subramanian and Andrew G. Webb*
Department of Electrical and Computer Engineering & Beckman Institute for Advanced Science and Technology,
University of Illinois at UrbanasChampaign, 405 North Mathews Avenue, Urbana, Illinois 61801
Typical high-resolution natural-abundance 13C NMR ex-
periments require milligrams of material. In this study,
solenoidal microcoil direct and inverse detection probes
have been designed to explore improvements in the limits
of detection (LOD) for 13C NMR at 11.7 T. A direct-
detection radio frequency probe with an observe volume
(Vobs) of 1 L has been developed with carbon observe,
deuterium lock, and proton decouple channels. The
nonspinning line width of a decoupled [3-13C]-L-alanine
(99%) peak achieved is 1.3 Hz. The natural-abundance
13C 3 LOD for sucrose for a 90-min acquisition time is
18 g (52 nmol). The 3 LOD for [3-13C]-L-alanine (99%)
is 375 ng (4.2 nmol) for an acquisition time less than 30
s. The inverse detection probe has proton observe and


Source: Andrews, Anne M. - Huck Institutes of the Life Sciences, Pennsylvania State University


Collections: Biology and Medicine