Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
Available online at www.sciencedirect.com Directed enzyme evolution: climbing fitness peaks one amino acid
 

Summary: Available online at www.sciencedirect.com
Directed enzyme evolution: climbing fitness peaks one amino acid
at a time
Cara A Tracewell1
and Frances H Arnold1,2
Directed evolution can generate a remarkable range of new
enzyme properties. Alternate substrate specificities and
reaction selectivities are readily accessible in enzymes from
families that are naturally functionally diverse. Activities on new
substrates can be obtained by improving variants with
broadened specificities or by step-wise evolution through a
sequence of more and more challenging substrates. Evolution
of highly specific enzymes has been demonstrated, even with
positive selection alone. It is apparent that many solutions exist
for any given problem, and there are often many paths that lead
uphill, one step at a time.
Addresses
1
Division of Chemistry & Chemical Engineering, California Institute of
Technology, Pasadena, CA 91125, USA

  

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

 

Collections: Chemistry; Biology and Medicine